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| import gradio as gr | |
| import joblib | |
| import numpy as np | |
| import pandas as pd | |
| from propy import AAComposition | |
| from sklearn.preprocessing import MinMaxScaler | |
| from fastapi import FastAPI | |
| from gradio.routes import mount_gradio_app | |
| # Load trained SVM model and scaler | |
| model = joblib.load("SVM.joblib") | |
| scaler = joblib.load("norm.joblib") | |
| # FastAPI instance | |
| app = FastAPI() | |
| # Selected features used in the model | |
| selected_features = [ | |
| "A", "R", "N", "D", "C", "E", "Q", "G", "H", "I", "L", "K", "M", "F", "P", "S", "T", "W", "Y", "V", | |
| "AA", "AR", "AN", "AD", "AC", "AE", "AQ", "AG", "AI", "AL", "AK", "AF", "AP", "AS", "AT", "AY", "AV", | |
| "RA", "RR", "RN", "RD", "RC", "RE", "RQ", "RG", "RH", "RI", "RL", "RK", "RM", "RF", "RS", "RT", "RY", "RV", | |
| "NA", "NR", "ND", "NC", "NE", "NG", "NI", "NL", "NK", "NP", | |
| "DA", "DR", "DN", "DD", "DC", "DE", "DQ", "DG", "DI", "DL", "DK", "DP", "DS", "DT", "DV", | |
| "CA", "CR", "CN", "CD", "CC", "CE", "CG", "CH", "CI", "CL", "CK", "CF", "CP", "CS", "CT", "CY", "CV", | |
| "EA", "ER", "EN", "ED", "EC", "EE", "EQ", "EG", "EI", "EL", "EK", "EP", "ES", "ET", "EV", | |
| "QA", "QR", "QC", "QG", "QL", "QK", "QP", "QT", "QV", | |
| "GA", "GR", "GD", "GC", "GE", "GQ", "GG", "GI", "GL", "GK", "GF", "GP", "GS", "GW", "GY", "GV", | |
| "HC", "HG", "HL", "HK", "HP", | |
| "IA", "IR", "ID", "IC", "IE", "II", "IL", "IK", "IF", "IP", "IS", "IT", "IV", | |
| "LA", "LR", "LN", "LD", "LC", "LE", "LQ", "LG", "LI", "LL", "LK", "LM", "LF", "LP", "LS", "LT", "LV", | |
| "KA", "KR", "KN", "KD", "KC", "KE", "KQ", "KG", "KH", "KI", "KL", "KK", "KM", "KF", "KP", "KS", "KT", "KV", | |
| "MA", "ME", "MI", "ML", "MK", "MF", "MP", "MS", "MT", "MV", | |
| "FR", "FC", "FQ", "FG", "FI", "FL", "FF", "FS", "FT", "FY", "FV", | |
| "PA", "PR", "PD", "PC", "PE", "PG", "PL", "PK", "PS", "PV", | |
| "SA", "SR", "SD", "SC", "SE", "SG", "SH", "SI", "SL", "SK", "SF", "SP", "SS", "ST", "SY", "SV", | |
| "TA", "TR", "TN", "TC", "TE", "TG", "TI", "TL", "TK", "TF", "TP", "TS", "TT", "TV", | |
| "WC", | |
| "YR", "YD", "YC", "YG", "YL", "YS", "YV", | |
| "VA", "VR", "VD", "VC", "VE", "VQ", "VG", "VI", "VL", "VK", "VP", "VS", "VT", "VY", "VV" | |
| ] | |
| def extract_features(sequence): | |
| """Extract only the required features and normalize them.""" | |
| all_features = AAComposition.CalculateAADipeptideComposition(sequence) | |
| feature_values = list(all_features.values()) | |
| feature_array = np.array(feature_values).reshape(-1, 1)[:420] # Ensure correct shape | |
| normalized_features = scaler.transform(feature_array.T).flatten() | |
| selected_feature_dict = { | |
| feature: normalized_features[i] | |
| for i, feature in enumerate(selected_features) | |
| if feature in all_features | |
| } | |
| selected_feature_df = pd.DataFrame([selected_feature_dict]) | |
| return selected_feature_df.to_numpy() | |
| def predict(sequence): | |
| """Predict AMP vs Non-AMP""" | |
| features = extract_features(sequence) | |
| prediction = model.predict(features.T)[0] | |
| return {"prediction": "AMP" if prediction == 0 else "Non-AMP"} | |
| # FastAPI endpoint | |
| async def predict_api(request: dict): | |
| sequence = request.get("sequence", "") | |
| if not sequence or len(sequence) < 10 or len(sequence) > 100: | |
| return {"error": "Sequence length must be between 10 and 100."} | |
| return predict(sequence) | |
| # Gradio Interface (optional if you want UI access) | |
| iface = gr.Interface( | |
| fn=predict, | |
| inputs=gr.Textbox(label="Enter Protein Sequence"), | |
| outputs=gr.Label(label="Prediction"), | |
| title="AMP Classifier", | |
| description="Enter an amino acid sequence to predict AMP or Non-AMP." | |
| ) | |
| app = mount_gradio_app(app, iface, path="/gradio") | |
| # Run the server with: `uvicorn filename:app --host 0.0.0.0 --port 7860` | |