id
stringlengths 7
10
| title
stringlengths 8
354
| content
stringlengths 91
4.1k
| contents
stringlengths 164
4.24k
| embedding
sequencelengths 384
384
|
|---|---|---|---|---|
PMID:27094 | Granulomatous ileocolitis preceded by three years of pauciarticular arthritis. | The case of a 12-year old male is presented in whom pauciarticular arthritis preceded the clinical onset of granulomatous ileocolitis by three years. Before the onset of gastrointestinal symptoms, the patients' clinical diagnosis was juvenile rheumatoid arthritis. This case stresses the importance of including inflammatory bowel disease in the differential diagnosis of pauciarticular arthritis in children. | Granulomatous ileocolitis preceded by three years of pauciarticular arthritis. The case of a 12-year old male is presented in whom pauciarticular arthritis preceded the clinical onset of granulomatous ileocolitis by three years. Before the onset of gastrointestinal symptoms, the patients' clinical diagnosis was juvenile rheumatoid arthritis. This case stresses the importance of including inflammatory bowel disease in the differential diagnosis of pauciarticular arthritis in children. | [
-0.03978261724114418,
-0.01095479167997837,
0.05124426260590553,
0.061476510018110275,
-0.008401341736316681,
-0.020979950204491615,
0.012804764322936535,
0.07271848618984222,
0.011130817234516144,
-0.014220742508769035,
0.03154781460762024,
-0.021508945152163506,
0.052453648298978806,
-0.015667248517274857,
-0.017530445009469986,
-0.010883507318794727,
0.03644276782870293,
-0.003972634673118591,
-0.056573428213596344,
-0.008837363682687283,
-0.010977585799992085,
0.011074235662817955,
0.04317629709839821,
-0.053593166172504425,
0.054121341556310654,
0.05284278094768524,
0.014255978167057037,
-0.00042017869418486953,
-0.03454645350575447,
-0.12663698196411133,
0.05839375779032707,
-0.0022539785131812096,
-0.002540350891649723,
-0.005191721022129059,
-0.010879170149564743,
-0.013041837140917778,
-0.06368625909090042,
0.024787910282611847,
0.0089560616761446,
0.005987552460283041,
0.004800262860953808,
0.016346167773008347,
-0.03711462393403053,
-0.023548293858766556,
-0.07720955461263657,
-0.019043460488319397,
-0.05360306054353714,
0.02341357246041298,
0.11972952634096146,
0.06718739867210388,
0.007377253379672766,
0.016362695023417473,
0.029160739853978157,
0.11529172956943512,
0.010608794167637825,
0.04614265263080597,
-0.004674308001995087,
-0.0009243960375897586,
-0.01043421495705843,
-0.010867641307413578,
0.04229048639535904,
0.0419570691883564,
-0.1264975666999817,
0.09755309671163559,
0.04076455160975456,
0.04007938131690025,
0.013350713066756725,
-0.046243567019701004,
0.023627830669283867,
-0.005584764759987593,
0.024441177025437355,
0.04434613510966301,
-0.061868663877248764,
0.06016479432582855,
-0.024603622034192085,
0.003923209849745035,
-0.04086785390973091,
-0.017824655398726463,
0.05944020673632622,
0.05981709808111191,
0.03928125649690628,
0.10536142438650131,
-0.02140103653073311,
-0.0004433242429513484,
-0.030813058838248253,
-0.05871262028813362,
-0.05078328028321266,
-0.07833296805620193,
-0.04602111503481865,
0.009312731213867664,
0.004226041492074728,
-0.06989838927984238,
-0.05100991949439049,
0.020178113132715225,
-0.04413323104381561,
0.033716410398483276,
-0.02341695874929428,
-0.046173904091119766,
-0.03653990849852562,
0.342495858669281,
-0.03351862356066704,
-0.028555380180478096,
0.03453037887811661,
0.009269542060792446,
0.02340662106871605,
0.05020947381854057,
0.044153518974781036,
-0.023087704554200172,
-0.04590192809700966,
-0.01807595230638981,
0.008267409168183804,
0.009546415880322456,
0.006906094029545784,
-0.029871463775634766,
0.04086918756365776,
-0.011544848792254925,
0.05627482756972313,
-0.03605140373110771,
-0.04674897715449333,
0.039385005831718445,
0.05008545145392418,
-0.008195201866328716,
-0.013313171453773975,
-0.02548503316938877,
0.021364765241742134,
0.03774852678179741,
0.05115750804543495,
0.06954512000083923,
-0.005855071824043989,
-0.018792010843753815,
0.04345489665865898,
-0.021783968433737755,
-0.019053258001804352,
-0.013225415721535683,
-0.0015619697514921427,
0.009418874979019165,
0.028669346123933792,
-0.011705627664923668,
0.023380553349852562,
0.045081425458192825,
-0.027994588017463684,
-0.09793069958686829,
-0.024067142978310585,
-0.03697916492819786,
-0.06395048648118973,
0.07287611812353134,
-0.03564682975411415,
-0.009545094333589077,
0.009193707257509232,
0.006857029162347317,
0.026415139436721802,
0.025293558835983276,
0.013364652171730995,
0.07943448424339294,
-0.010399623773992062,
0.04165161773562431,
0.010946957394480705,
0.044538382440805435,
0.002083483152091503,
0.014185229316353798,
-0.07710077613592148,
-0.03959628567099571,
-0.01519285049289465,
0.029357293620705605,
0.026557132601737976,
-0.03916950896382332,
-0.006845714058727026,
-0.009721050970256329,
0.011493961326777935,
0.07718899101018906,
0.0036438468378037214,
0.09120651334524155,
0.038776278495788574,
-0.004798482172191143,
0.019047042354941368,
0.07272122800350189,
0.01880294270813465,
0.0680447369813919,
0.0003045781922992319,
0.0029086570721119642,
0.004357663448899984,
-0.017692318186163902,
-0.01809166558086872,
0.06063375249505043,
-0.02382761426270008,
-0.011967288330197334,
-0.04437731206417084,
-0.004062122665345669,
0.07036395370960236,
-0.029363008216023445,
-0.01575613021850586,
-0.002717045834288001,
-0.07297706604003906,
-0.021009672433137894,
-0.027166323736310005,
-0.006882636807858944,
-0.03372148796916008,
-0.12135377526283264,
-0.0021141956094652414,
0.010991068556904793,
0.03931283950805664,
-0.02531491406261921,
0.08215511590242386,
-0.005943640135228634,
-0.02885350026190281,
0.013699735514819622,
0.05041620135307312,
-0.000650433823466301,
0.0055509149096906185,
0.014322508126497269,
-0.0287706907838583,
0.050565093755722046,
0.013449379242956638,
-0.012943381443619728,
-0.06151971220970154,
0.05204865708947182,
-0.05878419056534767,
-0.02106371708214283,
0.032176434993743896,
0.023031296208500862,
0.03498674929141998,
0.050279319286346436,
-0.023418588563799858,
-0.28235936164855957,
-0.01078384555876255,
-0.018724588677287102,
-0.022640950977802277,
0.031965289264917374,
-0.010447551496326923,
-0.04861372709274292,
-0.06020183116197586,
0.006789823528379202,
-0.0597822405397892,
0.02576366625726223,
0.1372818797826767,
-0.024232318624854088,
-0.045235659927129745,
-0.04740655794739723,
0.04028714820742607,
0.06953257322311401,
0.0320366732776165,
0.028917577117681503,
0.03116755001246929,
-0.027640478685498238,
-0.060866158455610275,
-0.010880939662456512,
0.0585312694311142,
0.009400959126651287,
0.01083517074584961,
0.14048358798027039,
0.046436239033937454,
-0.057841863483190536,
-0.12326663732528687,
0.03624889254570007,
-0.014826374128460884,
-0.008173948153853416,
0.009318667463958263,
0.0066643995232880116,
-0.051854439079761505,
-0.06643206626176834,
-0.08007676899433136,
-0.06356989592313766,
-0.04997649043798447,
-0.02283259853720665,
-0.023017626255750656,
0.0457962304353714,
-0.02788587659597397,
-0.07639322429895401,
-0.01109458226710558,
0.009791110642254353,
0.007023117505013943,
-0.008345844224095345,
-0.009744102135300636,
0.05519299954175949,
-0.03191019967198372,
-0.007221349515020847,
0.055139850825071335,
-0.04274536296725273,
-0.03779382258653641,
-0.00973836425691843,
0.00984033104032278,
-0.05510598421096802,
-0.002912836614996195,
-0.026950296014547348,
-0.01469467580318451,
0.05309073626995087,
-0.042582277208566666,
-0.013510049320757389,
0.006490401923656464,
0.00023928639711812139,
-0.05652899667620659,
-0.03225553408265114,
0.03262091800570488,
0.008615450002253056,
0.10229223221540451,
-0.07081867754459381,
-0.02724773995578289,
0.0058486685156822205,
0.039648402482271194,
-0.02430122159421444,
-0.056494828313589096,
-0.04992202669382095,
0.008641269989311695,
-0.0070969657972455025,
-0.06453380733728409,
0.07919930666685104,
0.000056932731240522116,
0.017255796119570732,
-0.03612528741359711,
-0.016480961814522743,
-0.006566562224179506,
-0.06338435411453247,
-0.025494569912552834,
0.020291157066822052,
0.03996703401207924,
-0.029820244759321213,
0.02482914738357067,
0.006792418193072081,
0.02901121973991394,
-0.30824318528175354,
0.08349362760782242,
-0.005277250427752733,
-0.04969092458486557,
-0.008510977029800415,
0.02578813210129738,
0.015840135514736176,
-0.006112321745604277,
-0.041760750114917755,
0.07316607981920242,
0.06776309013366699,
0.004949245136231184,
0.08962061256170273,
-0.051529839634895325,
-0.0109907491132617,
-0.030611347407102585,
0.06469562649726868,
-0.009689087979495525,
-0.042657505720853806,
-0.051882922649383545,
0.037710510194301605,
-0.03867388144135475,
0.11565793305635452,
0.04327143728733063,
-0.012501602061092854,
0.05312111973762512,
0.0014819195494055748,
0.03932086005806923,
-0.047610990703105927,
-0.012138159945607185,
0.012283947318792343,
-0.04374786093831062,
0.023502755910158157,
0.05903372913599014,
0.059869106858968735,
0.05593353882431984,
-0.0549018569290638,
0.06818784028291702,
-0.03808756172657013,
-0.01839563250541687,
-0.04269472137093544,
-0.01650635525584221,
0.011437704786658287,
-0.00016389103257097304,
0.02398078329861164,
0.0032328444067388773,
0.04708408564329147,
-0.026702886447310448,
-0.027888748794794083,
-0.011484972201287746,
-0.01532526034861803,
-0.024307316169142723,
0.053920358419418335,
0.046878132969141006,
0.06042522192001343,
0.003843186888843775,
-0.006177838426083326,
-0.010834265500307083,
-0.011818872764706612,
-0.08267060667276382,
0.05421745777130127,
-0.029642483219504356,
-0.05282163619995117,
0.036377985030412674,
-0.012136035598814487
] |
PMID:27097 | The influence of maternal psychological stress on the fetus. | The effects of maternal agitation, induced by exposure to bright light, upon fetal well-being were studied in pregnant rhesus monkeys at 139 to 148 days of gestation. Fetuses were classified as "healthy" or "asphyxiated" according to their initial acid-base state. Following variable periods of maternal excitement, a decrease in heart rate and arterial oxygenation was seen in all fetuses. Recovery occurred more rapidly in the healthy group, after maternal sedation was achieved, either by removing the stimulus or by additional administration of pentobarbital, 5 to 20 mg. intravenously. The beneficial effects of meternal sedation on the fetus have thus been demonstrated. | The influence of maternal psychological stress on the fetus. The effects of maternal agitation, induced by exposure to bright light, upon fetal well-being were studied in pregnant rhesus monkeys at 139 to 148 days of gestation. Fetuses were classified as "healthy" or "asphyxiated" according to their initial acid-base state. Following variable periods of maternal excitement, a decrease in heart rate and arterial oxygenation was seen in all fetuses. Recovery occurred more rapidly in the healthy group, after maternal sedation was achieved, either by removing the stimulus or by additional administration of pentobarbital, 5 to 20 mg. intravenously. The beneficial effects of meternal sedation on the fetus have thus been demonstrated. | [
0.009049282409250736,
0.061128728091716766,
0.02011924423277378,
0.08793645352125168,
-0.002009718446061015,
0.03963172435760498,
0.0855117216706276,
0.035039689391851425,
-0.007857762277126312,
0.018605422228574753,
-0.02913922630250454,
0.008584512397646904,
-0.03498319536447525,
0.02553069032728672,
-0.05463096871972084,
0.012254024855792522,
-0.011933594010770321,
-0.023911381140351295,
-0.09509401768445969,
0.03820529207587242,
-0.022701149806380272,
0.029262766242027283,
0.04654450714588165,
-0.005763719789683819,
0.03154701739549637,
0.005640579853206873,
-0.05623667314648628,
0.03549942001700401,
-0.05313757061958313,
-0.11737573891878128,
0.021049534901976585,
-0.007232513278722763,
0.07696443051099777,
0.0035839241463690996,
-0.012802519835531712,
0.03550279140472412,
-0.03329252079129219,
0.04668857529759407,
-0.08431679010391235,
0.060231007635593414,
0.08187679201364517,
0.026882195845246315,
-0.011895767413079739,
-0.004768974147737026,
-0.020475836470723152,
-0.016095153987407684,
0.04127012565732002,
-0.039556652307510376,
0.053770340979099274,
-0.01647133193910122,
0.025754695758223534,
-0.04566353186964989,
-0.005768992938101292,
0.08685504645109177,
-0.03957250714302063,
-0.04289704188704491,
0.04024045169353485,
-0.0091085871681571,
-0.002221350558102131,
0.027653072029352188,
-0.00971266906708479,
0.06285802274942398,
-0.14466650784015656,
0.046242307871580124,
0.03463263809680939,
-0.05123164504766464,
-0.038134243339300156,
-0.06389512121677399,
0.047187402844429016,
0.07501131296157837,
-0.04813796281814575,
0.021507376804947853,
0.06298375129699707,
0.004958217963576317,
0.022926680743694305,
-0.02934165671467781,
0.0776560828089714,
-0.05619784817099571,
0.024617129936814308,
0.015793778002262115,
-0.054491862654685974,
-0.05079961195588112,
-0.015521924942731857,
-0.03422796353697777,
0.04623052105307579,
-0.0316309854388237,
0.014034108258783817,
-0.04219941049814224,
-0.02811567299067974,
0.006003953516483307,
-0.04276086762547493,
-0.06845718622207642,
-0.013020873069763184,
-0.011872673407196999,
-0.024049943313002586,
-0.023588139563798904,
-0.0065235053189098835,
0.009794981218874454,
-0.038963060826063156,
0.38707220554351807,
-0.0326971597969532,
0.057863976806402206,
-0.060661520808935165,
0.005822452250868082,
0.017090830951929092,
-0.015571887604892254,
0.04458756744861603,
0.007631524465978146,
-0.008160236291587353,
0.018664192408323288,
0.018539799377322197,
-0.0009646675898693502,
0.059675753116607666,
-0.008152121677994728,
-0.012279664166271687,
-0.0139789879322052,
0.061252743005752563,
-0.02341127209365368,
0.07949695736169815,
0.03592195734381676,
0.0030541829764842987,
-0.015990519896149635,
0.06959041208028793,
-0.05839518457651138,
0.09195280820131302,
-0.019056076183915138,
-0.003534526564180851,
0.07860063016414642,
-0.027413954958319664,
-0.0691855251789093,
0.06013992801308632,
0.007187327835708857,
-0.025454113259911537,
0.03139793872833252,
0.01824779249727726,
-0.013814090751111507,
0.007562214974313974,
-0.0838572159409523,
-0.01652032509446144,
-0.03412274271249771,
0.005369374994188547,
-0.08190427720546722,
-0.017228666692972183,
-0.13197444379329681,
-0.028076142072677612,
0.0820363312959671,
0.04245967045426369,
0.012897545471787453,
-0.03379569575190544,
-0.007293898146599531,
-0.01489521935582161,
0.038230303674936295,
-0.001248211250640452,
0.01618178002536297,
-0.009766051545739174,
0.04965469241142273,
-0.03596847876906395,
0.0618169866502285,
0.01076978724449873,
-0.037301186472177505,
0.03132513910531998,
-0.050530970096588135,
-0.012015854008495808,
0.010638893581926823,
0.005935900844633579,
-0.0004755818226840347,
-0.02431372180581093,
-0.016243182122707367,
0.006450153421610594,
-0.03661734238266945,
0.024167567491531372,
-0.03010498732328415,
0.01643453910946846,
-0.010785920545458794,
0.004964526277035475,
-0.02828051522374153,
0.035866014659404755,
0.023793034255504608,
0.0056038484908640385,
0.006409008521586657,
0.07478112727403641,
-0.04106433317065239,
0.026349127292633057,
-0.032988041639328,
-0.0028118041809648275,
-0.03674469515681267,
-0.015130524523556232,
0.04132053256034851,
0.010232892818748951,
-0.0037503940984606743,
0.017827969044446945,
-0.09829223155975342,
-0.0017880531959235668,
-0.01995592936873436,
-0.021838990971446037,
-0.05458851158618927,
0.009363653138279915,
-0.041079260408878326,
-0.009673883207142353,
-0.0006913625984452665,
0.022823814302682877,
-0.029704837128520012,
-0.012739412486553192,
0.07126177102327347,
0.06207021698355675,
0.011285251006484032,
0.03693031892180443,
0.01734800636768341,
0.044095396995544434,
-0.05073128268122673,
-0.0021818061359226704,
0.03561200201511383,
0.04223674163222313,
-0.008463556878268719,
-0.05895661190152168,
0.05177060887217522,
-0.0014217982534319162,
0.011760705150663853,
0.027772407978773117,
0.03487208113074303,
0.025384020060300827,
-0.06934584677219391,
0.026127109304070473,
-0.3105677366256714,
-0.02044699341058731,
0.02699970453977585,
-0.03550785034894943,
0.03960514813661575,
-0.07977746427059174,
0.0391048863530159,
-0.08242784440517426,
0.024635445326566696,
-0.055565278977155685,
0.013396049849689007,
0.07256587594747543,
-0.05960126221179962,
0.03409489989280701,
-0.016615545377135277,
-0.02779330499470234,
0.02609291672706604,
-0.07465263456106186,
0.011095374822616577,
0.040146131068468094,
-0.027324944734573364,
0.05478375777602196,
-0.011905169114470482,
0.028248004615306854,
0.04735267162322998,
0.018585195764899254,
0.09019869565963745,
0.023109793663024902,
-0.0011322953505441546,
-0.014710736460983753,
-0.013354269787669182,
0.011546708643436432,
0.03750617802143097,
-0.1176336482167244,
0.031002527102828026,
0.03396446630358696,
0.027919543907046318,
0.044163696467876434,
-0.011085258796811104,
-0.016841614618897438,
-0.08067454397678375,
0.05877700075507164,
-0.012380016036331654,
-0.008178051561117172,
-0.0655343160033226,
-0.008949280716478825,
0.053167056292295456,
-0.022075148299336433,
-0.024947473779320717,
-0.028598206117749214,
0.015065048821270466,
-0.005863592028617859,
-0.030072877183556557,
-0.02992059290409088,
0.020682429894804955,
-0.059824779629707336,
-0.07436462491750717,
-0.0014293778222054243,
-0.09597085416316986,
-0.0354640856385231,
0.0025221058167517185,
-0.034924495965242386,
0.05317157506942749,
-0.07542261481285095,
-0.062121596187353134,
-0.003767416113987565,
0.025214750319719315,
-0.03290233016014099,
0.027444155886769295,
-0.015102778561413288,
-0.0388665646314621,
0.15882545709609985,
-0.06934014707803726,
-0.06100315973162651,
0.05164818838238716,
-0.02365560457110405,
-0.00809443835169077,
-0.02822338417172432,
-0.11813586205244064,
-0.0002335682074772194,
-0.026802491396665573,
0.0021318059880286455,
-0.0007045422098599374,
0.06014968827366829,
-0.012098715640604496,
-0.021013528108596802,
-0.009125685319304466,
-0.03020700253546238,
-0.0035535702481865883,
-0.017781857401132584,
-0.008292030543088913,
0.017019575461745262,
-0.04859153926372528,
-0.010788677260279655,
-0.0007942932425066829,
0.05655141547322273,
-0.2637435793876648,
-0.05786500498652458,
0.002955005271360278,
0.008399518206715584,
0.013153456151485443,
0.0011649341322481632,
-0.028348425403237343,
0.012227174825966358,
-0.003111487254500389,
0.025763632729649544,
-0.012565096840262413,
0.053909797221422195,
0.04238506779074669,
0.06533018499612808,
0.027430996298789978,
0.02147831954061985,
0.014479810371994972,
-0.005600426346063614,
-0.03646571561694145,
-0.022329557687044144,
0.00020887027494609356,
0.02021329291164875,
0.09864809364080429,
0.00958654098212719,
-0.0620817132294178,
0.0349477082490921,
-0.005543198436498642,
0.04381677135825157,
0.01471907738596201,
0.02886207215487957,
0.04229078069329262,
0.0175489354878664,
0.04980027675628662,
0.0035214668605476618,
0.051094066351652145,
0.004251573234796524,
-0.08057951927185059,
-0.037584107369184494,
0.04533451795578003,
0.01875160075724125,
0.011600345373153687,
0.02994893491268158,
0.024631686508655548,
-0.043390702456235886,
0.09987343102693558,
-0.01356465183198452,
-0.016963334754109383,
-0.061141058802604675,
0.013043439947068691,
0.06695155799388885,
-0.00854684878140688,
0.013230840675532818,
0.04457656666636467,
0.02328351140022278,
0.014610967598855495,
-0.01681499183177948,
0.028764141723513603,
0.028684822842478752,
0.036947738379240036,
0.026325246319174767,
-0.038559939712285995,
0.011710460297763348,
0.032940588891506195,
0.04879568889737129,
0.03291702643036842
] |
PMID:27099 | Gastric HCO3--secretion in the guinea pig. | Measurement of gastric intraluminal PCO2 and pH in the anesthetized guinea pig enabled simultaneous determination of total H+ and HCO3- gastric secretions. There was quantitative agreement between the release of CO2 and decrease in HCO3- after intragastric instillation of HCl. The basal rate of HCO3- secretion (approximately 40 mueq-h-1) was, in most cases, smaller than spontaneous H+ secretion, but gastric net secretory output was alkaline (HCO3- greater than H+) after inhibition of acid secretion with histamine H2-receptor antagonists (cimetidine 20 mg-kg-1 or metiamide 35 mg-kg-1). Carbachol (1-2 microgram-kg-1) stimulated secretion of both HCO3- and H+; only the latter response was sensitive to the histamine antagonists. Atropin (100 microgram-kg-1) blocked stimulation of HCO3- secretion but did not affect the basal output of HCO3-. An increase in HCO3- secretion was associated with an equivalent increase in net Na+ influx and an increase in the net influx of Cl- with H+ plus K+. Intragastric neutralization of H+ by HCO3- is likely to occur at the mucosal surface and may protect the mucosa from the damaging effects of intraluminal acid. | Gastric HCO3--secretion in the guinea pig. Measurement of gastric intraluminal PCO2 and pH in the anesthetized guinea pig enabled simultaneous determination of total H+ and HCO3- gastric secretions. There was quantitative agreement between the release of CO2 and decrease in HCO3- after intragastric instillation of HCl. The basal rate of HCO3- secretion (approximately 40 mueq-h-1) was, in most cases, smaller than spontaneous H+ secretion, but gastric net secretory output was alkaline (HCO3- greater than H+) after inhibition of acid secretion with histamine H2-receptor antagonists (cimetidine 20 mg-kg-1 or metiamide 35 mg-kg-1). Carbachol (1-2 microgram-kg-1) stimulated secretion of both HCO3- and H+; only the latter response was sensitive to the histamine antagonists. Atropin (100 microgram-kg-1) blocked stimulation of HCO3- secretion but did not affect the basal output of HCO3-. An increase in HCO3- secretion was associated with an equivalent increase in net Na+ influx and an increase in the net influx of Cl- with H+ plus K+. Intragastric neutralization of H+ by HCO3- is likely to occur at the mucosal surface and may protect the mucosa from the damaging effects of intraluminal acid. | [
-0.00994824804365635,
-0.010994981043040752,
-0.01914546824991703,
0.07960058003664017,
-0.006089258939027786,
0.006295816972851753,
0.057128939777612686,
-0.013398596085608006,
-0.03840288519859314,
-0.006064159795641899,
-0.004629232455044985,
-0.08775602281093597,
-0.03579280525445938,
0.0349283292889595,
0.04713965579867363,
-0.03202587366104126,
-0.023719346150755882,
-0.007106056436896324,
-0.08095595985651016,
-0.05282361060380936,
-0.0598934032022953,
-0.008854599669575691,
0.011925598606467247,
0.009966079145669937,
0.011701843701303005,
0.0012757419608533382,
-0.01686565950512886,
-0.007074097171425819,
-0.07225995510816574,
-0.20061743259429932,
0.04225602746009827,
-0.03455395624041557,
0.015304581262171268,
-0.03994600102305412,
-0.036152053624391556,
0.01880546659231186,
0.0009838702389970422,
0.05459318310022354,
-0.023860855028033257,
0.06554514169692993,
0.058335959911346436,
-0.010667849332094193,
0.024030480533838272,
-0.00747255701571703,
0.0106114586815238,
-0.03138536959886551,
-0.0017458547372370958,
0.00924842618405819,
0.08056478947401047,
0.00697496859356761,
0.03638260439038277,
0.055780790746212006,
0.008817884139716625,
0.05465373396873474,
0.0399295948445797,
-0.040188223123550415,
-0.0037998564075678587,
0.016804873943328857,
-0.02772519737482071,
-0.031014375388622284,
0.02123681828379631,
0.06609592586755753,
-0.1464664340019226,
0.014965257607400417,
0.11070896685123444,
-0.0030319250654429197,
-0.04550957307219505,
-0.047833845019340515,
0.03323796018958092,
0.09240096062421799,
-0.03718367591500282,
-0.013612347654998302,
-0.016790764406323433,
0.02396547608077526,
0.020752262324094772,
-0.04201211780309677,
0.012808709405362606,
-0.01304531004279852,
-0.0062162610702216625,
-0.027727030217647552,
0.005881913006305695,
0.00936012715101242,
-0.028538310900330544,
-0.0029595629312098026,
0.013275873847305775,
-0.05625113472342491,
0.04279109835624695,
-0.002220327965915203,
-0.043825022876262665,
-0.01005860697478056,
-0.02085346356034279,
-0.08747315406799316,
-0.0304748322814703,
-0.01854380965232849,
-0.059830985963344574,
0.0065585956908762455,
0.041580505669116974,
-0.057199690490961075,
-0.03423021361231804,
0.3480842709541321,
-0.005091798026114702,
-0.03798901289701462,
-0.06312349438667297,
-0.03764016181230545,
0.06459701061248779,
-0.017502209171652794,
0.02937752567231655,
0.054933253675699234,
-0.007039329502731562,
0.043681591749191284,
0.08128435164690018,
0.056718915700912476,
0.038068998605012894,
-0.06644457578659058,
-0.015520940534770489,
0.019571363925933838,
0.054622747004032135,
0.031216612085700035,
0.06007172912359238,
0.0419471450150013,
0.00301166414283216,
0.00525660952553153,
0.05118981748819351,
0.013073081150650978,
0.033342793583869934,
-0.004832242149859667,
-0.021865610033273697,
0.0726131722331047,
-0.008042181842029095,
0.01924493908882141,
0.05358682572841644,
0.019500739872455597,
-0.021209264174103737,
0.01254366897046566,
-0.02943616360425949,
-0.032470040023326874,
0.02439207397401333,
0.026052024215459824,
0.03091786615550518,
-0.0161247868090868,
-0.06607178598642349,
-0.05062813311815262,
-0.02588791213929653,
-0.11736463010311127,
-0.05074092000722885,
0.12255984544754028,
0.005009832791984081,
0.010495425201952457,
-0.02614849992096424,
-0.02568674273788929,
0.01989821530878544,
0.03928685560822487,
0.010058555752038956,
0.05979568883776665,
-0.014081552624702454,
-0.019566545262932777,
-0.026338806375861168,
0.02063986472785473,
-0.008633260615170002,
-0.00658073415979743,
-0.06874735653400421,
-0.041358545422554016,
-0.02251707762479782,
0.057382624596357346,
0.030161436647176743,
-0.008629970252513885,
0.011314638890326023,
-0.011943352408707142,
0.03263075277209282,
0.05118556693196297,
-0.003932453691959381,
0.05994896963238716,
-0.06828755140304565,
-0.06317340582609177,
0.02646789327263832,
0.009371104650199413,
0.03192733973264694,
0.029602639377117157,
0.07113617658615112,
-0.011198476888239384,
0.005135951563715935,
0.030807824805378914,
-0.04083219915628433,
0.00957509782165289,
0.011427524499595165,
0.02493261732161045,
-0.08404531329870224,
0.06448397785425186,
-0.008476370014250278,
0.08680783957242966,
-0.03744303062558174,
0.010482029058039188,
-0.09952008724212646,
-0.014077980071306229,
-0.03382237255573273,
-0.0421462245285511,
-0.008825116790831089,
-0.0336538702249527,
-0.037397824227809906,
0.003312094835564494,
0.07188557088375092,
0.023198649287223816,
0.02469586580991745,
0.036100517958402634,
-0.025440676137804985,
0.0016201038379222155,
-0.00955505296587944,
-0.022363414987921715,
0.05760856345295906,
-0.03672752529382706,
-0.0018480601720511913,
0.07226908206939697,
-0.02584121935069561,
0.014602871611714363,
-0.022281654179096222,
0.030890876427292824,
0.020275628194212914,
-0.016762053593993187,
0.016075946390628815,
0.0352451466023922,
0.029162587597966194,
-0.038734324276447296,
-0.04313971474766731,
-0.30105283856391907,
-0.030784988775849342,
0.043227240443229675,
-0.035225652158260345,
0.11176007241010666,
-0.026877032592892647,
0.05122128129005432,
-0.012344280257821083,
-0.004058098886162043,
-0.06914559006690979,
0.04279045760631561,
0.05357613414525986,
-0.06668199598789215,
0.0038377654273062944,
0.004218911752104759,
0.041444890201091766,
0.03352377563714981,
-0.01790999062359333,
0.05417086184024811,
0.0468655563890934,
0.07710769027471542,
-0.010261275805532932,
-0.015176123939454556,
-0.009695228189229965,
-0.008862501010298729,
0.008208814077079296,
0.09842583537101746,
-0.004167400766164064,
0.04218466952443123,
-0.06412091106176376,
0.010025032795965672,
0.03363252431154251,
-0.005444352049380541,
-0.06109750643372536,
-0.016145700588822365,
-0.025469772517681122,
-0.03258484601974487,
-0.017182517796754837,
-0.024401208385825157,
-0.04009386897087097,
-0.03401380777359009,
-0.018725605681538582,
-0.018699783831834793,
-0.03492153063416481,
0.01875457540154457,
-0.06293541193008423,
-0.026459604501724243,
0.0826730877161026,
-0.004383410792797804,
-0.01047596987336874,
0.10101529955863953,
0.03256658837199211,
-0.03526365011930466,
-0.012196410447359085,
-0.0045464360155165195,
-0.04241296276450157,
0.001743977190926671,
0.028695015236735344,
-0.10661787539720535,
-0.0018780379323288798,
-0.014515833929181099,
0.009116810746490955,
-0.012809534557163715,
0.03862036392092705,
-0.05790949985384941,
0.02789633721113205,
0.06304451823234558,
-0.0034839995205402374,
0.06103353947401047,
0.018703125417232513,
0.05893731862306595,
0.11975456029176712,
-0.038351453840732574,
-0.002287206705659628,
0.08314602822065353,
0.014374329708516598,
0.013769621029496193,
-0.08707959204912186,
-0.07076971232891083,
-0.014023914001882076,
0.01159044448286295,
-0.04586337134242058,
0.05037756636738777,
0.0475291982293129,
-0.03900640830397606,
0.04828431457281113,
0.015638787299394608,
-0.025117890909314156,
0.036237794905900955,
0.04036767780780792,
-0.0019168335711583495,
0.03357139229774475,
-0.030209103599190712,
-0.09678030759096146,
0.01271287351846695,
0.02915959246456623,
-0.2575235366821289,
-0.026962844654917717,
-0.011103020049631596,
-0.006322775036096573,
0.016670556738972664,
0.010189346969127655,
0.04632210358977318,
-0.04603928327560425,
-0.04971944913268089,
-0.010358482599258423,
0.007556689437478781,
0.012381821870803833,
0.013132155872881413,
0.0664147436618805,
-0.01993750035762787,
0.006373864132910967,
-0.007148388773202896,
-0.030680213123559952,
-0.020042408257722855,
0.033366329967975616,
0.016138484701514244,
-0.055728886276483536,
0.11767135560512543,
0.024827200919389725,
-0.016994992271065712,
0.026988361030817032,
0.017045754939317703,
0.013144543394446373,
0.03507188707590103,
0.018264977261424065,
0.0019781894516199827,
0.00972825475037098,
0.03847995400428772,
0.06305646896362305,
0.05437343940138817,
0.006224706768989563,
-0.032311949878931046,
0.018422285094857216,
-0.05097503215074539,
0.04981140047311783,
-0.05699737370014191,
0.010518569499254227,
-0.07837950438261032,
-0.07576218992471695,
0.05744057521224022,
-0.056359462440013885,
0.012316991575062275,
-0.07059351354837418,
0.023443814367055893,
-0.023316264152526855,
-0.01315605640411377,
-0.0849502757191658,
0.06204020604491234,
0.03669985383749008,
0.04287867620587349,
-0.00813437532633543,
0.009661182761192322,
-0.06774075329303741,
-0.025175422430038452,
0.03190256655216217,
-0.0013457377208396792,
-0.041674405336380005,
-0.0005192541284486651,
0.037459149956703186,
0.023773588240146637
] |
PMID:27100 | Acetylsalicylic acid hydrolase of gastric mucosa. | Acetylsalicylic acid hydrolase activity of rabbit fundic gastric mucosa has been isolated from the soluble 100,000 X g supernate. The enzymatic activity was partially purified by ammonium sulfate precipitation. The Km for acetylsalicylate was 2 mM and pH optimum was 8.6. The activity was insensitive to ionic strength, slightly inhibited by inclusion of 100 mM Cl-, and demonstrated no requirement for Ca2+ or Mg2+. Acetylsalicylic acid esterase was markedly inhibited by sodium cholate and sodium dodecyl sulfate. The enzyme was insensitive to sulfhydryl reagents with the exception of p-chloromercuribenzenesulfonic acid, which markedly inhibited the enzyme. Diisopropyl fluorophosphate (DFP) inhibited enzymatic activity with a Ki of 9 X 10(-9)M. Eserine was also inhibitory with a Ki of 0.25 mM. Inhibition by DFP at low concentration and by eserine at millimolar concentrations suggests that this enzyme is related to the group of aliphatic esterases. Identification of potent inhibitors will enable studies to define the role of this enzyme with the use of experimental preparations in which systemic toxicity can be avoided. | Acetylsalicylic acid hydrolase of gastric mucosa. Acetylsalicylic acid hydrolase activity of rabbit fundic gastric mucosa has been isolated from the soluble 100,000 X g supernate. The enzymatic activity was partially purified by ammonium sulfate precipitation. The Km for acetylsalicylate was 2 mM and pH optimum was 8.6. The activity was insensitive to ionic strength, slightly inhibited by inclusion of 100 mM Cl-, and demonstrated no requirement for Ca2+ or Mg2+. Acetylsalicylic acid esterase was markedly inhibited by sodium cholate and sodium dodecyl sulfate. The enzyme was insensitive to sulfhydryl reagents with the exception of p-chloromercuribenzenesulfonic acid, which markedly inhibited the enzyme. Diisopropyl fluorophosphate (DFP) inhibited enzymatic activity with a Ki of 9 X 10(-9)M. Eserine was also inhibitory with a Ki of 0.25 mM. Inhibition by DFP at low concentration and by eserine at millimolar concentrations suggests that this enzyme is related to the group of aliphatic esterases. Identification of potent inhibitors will enable studies to define the role of this enzyme with the use of experimental preparations in which systemic toxicity can be avoided. | [
-0.07901328802108765,
-0.04490593075752258,
-0.07844173908233643,
0.06712937355041504,
0.013993914239108562,
-0.02383071556687355,
0.003582051722332835,
0.07419104874134064,
-0.05874071642756462,
-0.038397204130887985,
0.03085933066904545,
-0.10858578234910965,
-0.004256458953022957,
-0.007225347682833672,
-0.03548435866832733,
-0.022593315690755844,
0.0216620285063982,
-0.02834637276828289,
-0.0626540407538414,
-0.0043035545386374,
0.026793815195560455,
0.030584197491407394,
-0.00555336382240057,
-0.02800818532705307,
0.030239004641771317,
0.07026571035385132,
0.04377717524766922,
-0.056568022817373276,
-0.06095869839191437,
-0.17535987496376038,
0.0013870070688426495,
-0.012142372317612171,
0.010159502737224102,
-0.025306617841124535,
-0.015576448291540146,
-0.0023889783769845963,
-0.041012953966856,
0.03396770730614662,
-0.0045834071934223175,
0.041361212730407715,
0.0013775626430287957,
0.014520322903990746,
-0.0023452360183000565,
0.03425024077296257,
-0.015074864961206913,
0.00379465171135962,
-0.03789816424250603,
0.0019006270449608564,
0.049278125166893005,
0.006702808663249016,
-0.008289137855172157,
0.036406032741069794,
-0.053425248712301254,
0.018732968717813492,
0.043563492596149445,
-0.006574292201548815,
0.030711248517036438,
-0.016485847532749176,
0.03978414088487625,
-0.03439778462052345,
-0.011057304218411446,
-0.000992755638435483,
-0.12403044104576111,
0.05168621614575386,
0.009895654395222664,
0.028892159461975098,
-0.01062571257352829,
-0.014048879966139793,
0.11241506785154343,
0.07418696582317352,
-0.07567522674798965,
0.04350681230425835,
-0.021638885140419006,
0.0130835035815835,
-0.035769134759902954,
0.0032759206369519234,
0.06931546330451965,
0.042012374848127365,
0.033457811921834946,
0.05900990962982178,
0.01303086057305336,
0.01446820329874754,
0.02114769257605076,
-0.011398369446396828,
0.013608541339635849,
0.03905360400676727,
0.0369572788476944,
0.01076969038695097,
0.011355136521160603,
-0.01195245236158371,
0.0001994726189877838,
-0.07379821687936783,
-0.02030552737414837,
-0.06895412504673004,
-0.05581796169281006,
0.06606446951627731,
0.02088434249162674,
0.0020147014874964952,
0.010880902409553528,
0.3327731192111969,
-0.053630124777555466,
-0.02711184322834015,
-0.07054812461137772,
-0.02029278129339218,
0.010408719070255756,
-0.0005971476202830672,
-0.013240300118923187,
0.03617529943585396,
-0.015909764915704727,
-0.004072048235684633,
0.11354663223028183,
0.03057195246219635,
-0.00882916059345007,
-0.046662747859954834,
-0.016146911308169365,
0.013348822481930256,
0.04646860435605049,
-0.007355866488069296,
-0.00277676060795784,
0.06359860301017761,
-0.006679008714854717,
-0.035723332315683365,
0.027186404913663864,
-0.010189411230385303,
0.009235563687980175,
0.023864900693297386,
-0.047855377197265625,
0.08215819299221039,
0.0818459540605545,
-0.012948861345648766,
-0.009033669717609882,
0.009601123631000519,
-0.00019822971080429852,
0.021823428571224213,
0.001049477607011795,
-0.041839975863695145,
0.05057254433631897,
0.0462406761944294,
0.0626349002122879,
-0.05811113491654396,
-0.03523261472582817,
-0.03300301358103752,
-0.040445975959300995,
-0.029349567368626595,
-0.016394982114434242,
0.07763808220624924,
0.008485575206577778,
-0.013174551539123058,
-0.07716605067253113,
0.021652238443493843,
-0.0088298125192523,
-0.022991638630628586,
0.03143215924501419,
0.1270180195569992,
0.013662769459187984,
0.07483969628810883,
0.008409995585680008,
0.02745344489812851,
0.014784842729568481,
0.011303196661174297,
-0.02748996764421463,
-0.040754783898591995,
-0.006699592340737581,
0.05136704072356224,
0.01196811068803072,
-0.008335811085999012,
-0.019259091466665268,
0.03138277307152748,
-0.03361809626221657,
0.016642941161990166,
0.0008950866758823395,
-0.008740723133087158,
-0.0351906456053257,
-0.026808617636561394,
-0.06165073812007904,
0.043733373284339905,
0.015391719527542591,
0.016964511945843697,
-0.004473322536796331,
0.011837397702038288,
0.029691973701119423,
0.006484758574515581,
0.01842484064400196,
0.011121812276542187,
-0.008813285268843174,
-0.03562155365943909,
-0.019173910841345787,
-0.05717843770980835,
0.05788959190249443,
0.018432525917887688,
-0.04998243600130081,
0.010827195830643177,
-0.1158510074019432,
-0.030320890247821808,
-0.0651734322309494,
-0.022950507700443268,
-0.032193876802921295,
0.011628827080130577,
0.00444759102538228,
0.0371265783905983,
0.10984829068183899,
0.07523537427186966,
-0.000593693635892123,
0.09352660924196243,
0.05961969867348671,
0.06721990555524826,
-0.041212525218725204,
0.02057444117963314,
-0.03976469486951828,
-0.028083812445402145,
-0.015339224599301815,
-0.007059866562485695,
-0.05958333611488342,
0.02267269790172577,
-0.06743473559617996,
0.08640487492084503,
0.02532452717423439,
0.003974840510636568,
0.07183140516281128,
-0.011177396401762962,
0.042012833058834076,
-0.0010184877319261432,
-0.07181958854198456,
-0.3018015921115875,
-0.042253002524375916,
-0.034105245023965836,
-0.009902182035148144,
0.07185625284910202,
-0.05868488550186157,
0.023865850642323494,
-0.05107440799474716,
-0.0014517306117340922,
-0.05971241369843483,
0.046939004212617874,
0.05136619508266449,
0.020854858681559563,
-0.026859980076551437,
-0.004335605539381504,
0.05751657485961914,
-0.010984791442751884,
-0.06067908927798271,
0.08211079984903336,
-0.02174151875078678,
0.04892776533961296,
-0.05511000379920006,
0.02844352275133133,
-0.012417423538863659,
0.07529841363430023,
0.009189315140247345,
0.09659881144762039,
-0.008325639180839062,
-0.001801368547603488,
-0.06398473680019379,
0.05300825834274292,
0.08265681564807892,
-0.017317041754722595,
-0.08968736231327057,
-0.038869939744472504,
-0.050261642783880234,
-0.009152760729193687,
-0.09401930123567581,
0.0331740640103817,
0.00004225327938911505,
0.0026279627345502377,
-0.02698904648423195,
0.00611438974738121,
-0.03239286318421364,
-0.01749110221862793,
0.005047036334872246,
-0.02887072041630745,
0.0268311258405447,
-0.04231419786810875,
-0.0340828113257885,
0.029200002551078796,
-0.016043752431869507,
0.02242220565676689,
0.004148100968450308,
-0.01846219412982464,
0.039496853947639465,
-0.0064182342030107975,
0.030192332342267036,
-0.051484718918800354,
-0.0482107438147068,
-0.03136580064892769,
0.03810245916247368,
-0.007178569212555885,
0.03891056403517723,
0.038475751876831055,
0.031513188034296036,
0.014383205212652683,
0.028480838984251022,
-0.01919722929596901,
0.019157135859131813,
-0.0015824923757463694,
0.09289964288473129,
-0.06905712187290192,
-0.04430500790476799,
0.05783281847834587,
0.03693307936191559,
0.0017003749962896109,
-0.05115219205617905,
-0.08183566480875015,
0.002773649524897337,
-0.015545402653515339,
-0.040705785155296326,
0.06330839544534683,
-0.05410631373524666,
-0.03452953323721886,
0.0035239721182733774,
0.017216423526406288,
-0.02966298907995224,
0.002072792500257492,
0.01197338942438364,
0.03546905517578125,
0.00840554479509592,
-0.04031232371926308,
0.0061933668330311775,
0.07635965198278427,
-0.04324154555797577,
-0.25329479575157166,
0.014907661825418472,
0.009737628512084484,
-0.02841372787952423,
0.014231673441827297,
0.026813620701432228,
0.06961684674024582,
-0.03264531120657921,
0.006088857538998127,
0.004957976285368204,
0.03134382888674736,
0.040831904858350754,
0.04607709124684334,
0.03675523400306702,
-0.007651750463992357,
-0.02834474854171276,
0.049257565289735794,
0.00518758175894618,
0.06507109850645065,
0.005523416213691235,
0.025605639442801476,
-0.053294986486434937,
0.15660126507282257,
0.03998594358563423,
-0.010982546955347061,
0.04096686467528343,
-0.0122569240629673,
-0.03787604346871376,
-0.02748534269630909,
-0.0016167559660971165,
-0.016681881621479988,
-0.0022071816492825747,
0.045412417501211166,
-0.03667435422539711,
0.04897477477788925,
0.0922706350684166,
-0.01673257350921631,
-0.02925526537001133,
0.001214421819895506,
0.013052569702267647,
-0.005678919609636068,
-0.00022566114785149693,
-0.08473331481218338,
-0.034815821796655655,
0.033817108720541,
-0.09012974798679352,
0.003993266262114048,
-0.07704931497573853,
0.02324661798775196,
-0.008802787400782108,
0.009291172958910465,
-0.13021647930145264,
0.04637177661061287,
0.03598174825310707,
0.010213589295744896,
-0.07181510329246521,
-0.006664856802672148,
0.01056276448071003,
-0.03867349401116371,
-0.01512689795345068,
-0.0072964876890182495,
-0.03492942452430725,
0.0019524492090567946,
0.07165203988552094,
0.052325110882520676
] |
PMID:27101 | Alterations of glucose metabolism during perfusion of rat lung with paraquat. | Isolated perfused rat lungs were used to investigate effects of paraquat on lung glucose metabolism. Lungs were ventilated with 5% CO2 in air and perfused with Krebs-Ringer bicarbonate buffer, pH 7.4, containing albumin and 5.5 mM radiolabeled D-glucose. Control lung glucose utilization, estimated from rate of 3H2O production from [5-3H]glucose, was 44 mumol/h-g dry wt. Pentose cycle activity, based on 14CO2 specific yields at the end of perfusions with [1-14C]- and [6-14C]glucose, was 14% of glucose utilization. During perfusion with 1.5 mM paraquat, glucose utilization increased 28%, 14CO2 production via the pentose cycle increased 182% (P less than 0.005), CO2 production via mitochondrial metabolism increased 39% (P less than 0.02), and the rate of lactate production increased 28% (P less than 0.05). Pyruvate production and the lactate-to-pyruvate ratio were not significantly altered. The data indicate that interaction of paraquat with the lung results in increased turnover of cytoplasmic NADPH and increased mitochondrial metabolism, but no significant change in cytoplasmic redox state. The findings are compatible with intracellular enzymatic reduction of paraquat by an NADPH-requiring reductase. | Alterations of glucose metabolism during perfusion of rat lung with paraquat. Isolated perfused rat lungs were used to investigate effects of paraquat on lung glucose metabolism. Lungs were ventilated with 5% CO2 in air and perfused with Krebs-Ringer bicarbonate buffer, pH 7.4, containing albumin and 5.5 mM radiolabeled D-glucose. Control lung glucose utilization, estimated from rate of 3H2O production from [5-3H]glucose, was 44 mumol/h-g dry wt. Pentose cycle activity, based on 14CO2 specific yields at the end of perfusions with [1-14C]- and [6-14C]glucose, was 14% of glucose utilization. During perfusion with 1.5 mM paraquat, glucose utilization increased 28%, 14CO2 production via the pentose cycle increased 182% (P less than 0.005), CO2 production via mitochondrial metabolism increased 39% (P less than 0.02), and the rate of lactate production increased 28% (P less than 0.05). Pyruvate production and the lactate-to-pyruvate ratio were not significantly altered. The data indicate that interaction of paraquat with the lung results in increased turnover of cytoplasmic NADPH and increased mitochondrial metabolism, but no significant change in cytoplasmic redox state. The findings are compatible with intracellular enzymatic reduction of paraquat by an NADPH-requiring reductase. | [
-0.04324570298194885,
-0.023583916947245598,
-0.058135636150836945,
0.04224611073732376,
0.0632343590259552,
-0.003180338069796562,
0.036977484822273254,
0.033658962696790695,
-0.04878787696361542,
0.0037750080227851868,
-0.02819756045937538,
-0.053150277584791183,
0.002988122869282961,
0.06932857632637024,
-0.006675835698843002,
-0.014547951519489288,
0.02487965114414692,
-0.01617453619837761,
-0.11854138970375061,
-0.015709513798356056,
-0.02095898985862732,
-0.01123211719095707,
0.009533005766570568,
-0.012207590974867344,
0.024752063676714897,
0.02360355108976364,
-0.02143760211765766,
0.01836458407342434,
-0.039655283093452454,
-0.17808730900287628,
0.009884326718747616,
0.03705575689673424,
0.012257305905222893,
-0.018807006999850273,
-0.03382403776049614,
0.005748271010816097,
0.020514048635959625,
0.029315682128071785,
-0.032197944819927216,
0.056623782962560654,
0.06897273659706116,
0.02220732718706131,
0.014734407886862755,
0.041832346469163895,
-0.007250285241752863,
0.006310042459517717,
-0.0647573471069336,
0.012483414262533188,
0.1211782842874527,
-0.05480087921023369,
0.08216580748558044,
-0.03275135159492493,
0.0258163008838892,
0.037010010331869125,
-0.0014861851232126355,
-0.02472115121781826,
0.03723251819610596,
0.03530169650912285,
-0.009786424227058887,
0.006902883294969797,
0.02342088893055916,
0.06942322105169296,
-0.13243889808654785,
0.027246301993727684,
0.03919374197721481,
-0.05182095989584923,
-0.0724513903260231,
-0.028917668387293816,
0.006772878114134073,
0.051929932087659836,
-0.06739649921655655,
0.009159383364021778,
-0.014878138899803162,
-0.017488857731223106,
0.022262098267674446,
-0.05262031406164169,
-0.007786967325955629,
-0.04908554255962372,
-0.01892312616109848,
0.06317564100027084,
0.033579133450984955,
-0.0005215966375544667,
-0.024908507242798805,
-0.00022993632592260838,
-0.025681043043732643,
-0.039496809244155884,
0.006827979814261198,
-0.035294558852910995,
-0.0085268784314394,
0.017778746783733368,
-0.034460436552762985,
-0.06548214703798294,
-0.06465496122837067,
0.011443262919783592,
-0.07694186270236969,
0.004697354976087809,
0.049907222390174866,
-0.002830064157024026,
-0.033220324665308,
0.37780165672302246,
0.025621717795729637,
0.03957315906882286,
-0.0223256666213274,
-0.010068688541650772,
-0.03825991228222847,
-0.06662793457508087,
-0.007724734954535961,
-0.015334934927523136,
0.049649011343717575,
0.025593306869268417,
0.04692359268665314,
0.013284497894346714,
-0.003642226103693247,
-0.0017593129305168986,
-0.008917837403714657,
0.025055978447198868,
0.027836007997393608,
0.03018200956285,
-0.0462036095559597,
0.04850371181964874,
0.007812419906258583,
-0.014689914882183075,
-0.005504827946424484,
0.04516832157969475,
0.03907235339283943,
0.01616431027650833,
0.008521096780896187,
0.09612812101840973,
0.0434722937643528,
0.05194193124771118,
0.09699418395757675,
0.0959208682179451,
0.009643458761274815,
-0.034342505037784576,
-0.035611432045698166,
-0.01572808250784874,
0.0495310053229332,
-0.014521320350468159,
0.05016530305147171,
0.011767299845814705,
-0.015965819358825684,
-0.046330757439136505,
-0.02226160652935505,
-0.055103495717048645,
-0.038750436156988144,
0.06248098984360695,
0.07492050528526306,
0.01995095983147621,
0.0008691238472238183,
-0.07027259469032288,
0.031240468844771385,
0.022152921184897423,
-0.020007343962788582,
0.009581845253705978,
0.006629963871091604,
0.026191353797912598,
-0.012847180478274822,
0.0006108250818215311,
0.10561098903417587,
-0.052498750388622284,
-0.05250122398138046,
-0.03364477679133415,
-0.0372421070933342,
0.04995150864124298,
0.0635809600353241,
0.03591432422399521,
-0.028556056320667267,
0.012723399326205254,
0.04567129164934158,
0.004344743210822344,
0.038303256034851074,
-0.04820401594042778,
-0.01825523190200329,
-0.09254907816648483,
0.02367570623755455,
0.04052508622407913,
0.03860090300440788,
0.04701602831482887,
0.0054678963497281075,
0.024667754769325256,
0.027353597804903984,
-0.04066530242562294,
0.04535691440105438,
0.007063578348606825,
0.003929374273866415,
-0.03439686447381973,
-0.03574303537607193,
-0.00017509367899037898,
0.027543507516384125,
0.02356347255408764,
-0.0689888522028923,
-0.0042891534976661205,
-0.17224323749542236,
-0.007917415350675583,
-0.008838252164423466,
-0.0355156734585762,
-0.032607149332761765,
0.02794489450752735,
0.015864809975028038,
-0.028709160163998604,
0.09880219399929047,
0.0007638541865162551,
0.07072405517101288,
0.019601700827479362,
0.03831041231751442,
0.029803801327943802,
-0.01170324720442295,
0.03736889362335205,
0.008325190283358097,
0.0278848335146904,
-0.03194386884570122,
0.020915968343615532,
-0.04201635718345642,
0.057814884930849075,
-0.052524566650390625,
0.053988266736269,
0.00850575789809227,
0.014924000948667526,
0.022815745323896408,
0.0546567365527153,
0.008582452312111855,
-0.0845760777592659,
-0.05658739432692528,
-0.31084486842155457,
-0.06578933447599411,
0.041088998317718506,
0.0029849912971258163,
0.06754475831985474,
-0.001256139948964119,
-0.02016288973391056,
-0.00025102743529714644,
-0.02465105801820755,
0.004734911490231752,
0.04999246075749397,
0.0464155338704586,
-0.008755579590797424,
0.00011879516387125477,
0.020427333191037178,
-0.013185315765440464,
0.041027382016181946,
-0.04903208464384079,
-0.008857324719429016,
-0.024437878280878067,
-0.007959485985338688,
-0.035333942621946335,
0.007579140365123749,
-0.06454409658908844,
-0.004235843196511269,
-0.02386242151260376,
0.0986725315451622,
0.002079179510474205,
0.0048177228309214115,
0.008078834973275661,
0.06063572317361832,
0.0362626276910305,
-0.021593676880002022,
-0.07139866054058075,
-0.04034341126680374,
-0.03826630488038063,
0.004327251110225916,
0.02503284253180027,
-0.01832042820751667,
-0.0319625623524189,
-0.07923398166894913,
-0.010362432338297367,
-0.02150929719209671,
-0.05809769034385681,
-0.07010886818170547,
-0.022460855543613434,
-0.04289297014474869,
0.015523593872785568,
0.002046337816864252,
-0.0124734528362751,
0.010015192441642284,
0.06507963687181473,
0.008423186838626862,
-0.0909888818860054,
0.07207011431455612,
-0.04942357540130615,
0.0011317009339109063,
0.0032669552601873875,
-0.085306815803051,
-0.012172636575996876,
-0.028049862012267113,
-0.018270952627062798,
-0.01928696408867836,
0.00012184123625047505,
0.010346053168177605,
-0.002085780957713723,
-0.04276786744594574,
-0.012375174090266228,
0.03680995851755142,
0.04911208152770996,
0.05478959530591965,
0.08950838446617126,
0.015421751886606216,
0.005982597358524799,
0.06433659046888351,
0.05457964539527893,
-0.05061452463269234,
0.0029817898757755756,
-0.05884501710534096,
-0.006936254911124706,
-0.010627439245581627,
0.004336229525506496,
0.07348193228244781,
0.008223411627113819,
-0.052974503487348557,
-0.009745988994836807,
0.006264394614845514,
-0.0008577800472266972,
0.07015996426343918,
-0.012227569706737995,
-0.011071629822254181,
0.01870768889784813,
-0.020447172224521637,
-0.08137541264295578,
0.027860894799232483,
0.053212009370326996,
-0.26531413197517395,
0.02900911122560501,
0.015565353445708752,
-0.07059813290834427,
0.015335405245423317,
0.0004007946408819407,
0.0024493185337632895,
0.03955649212002754,
0.000026492325559956953,
0.05500499904155731,
0.03656167536973953,
0.020097482949495316,
0.05190976709127426,
0.015741489827632904,
-0.008740568533539772,
0.02438972517848015,
-0.013306595385074615,
-0.024215947836637497,
-0.018298625946044922,
0.006075725425034761,
0.004046206828206778,
-0.06881635636091232,
0.12809434533119202,
0.007248620968312025,
-0.0386672168970108,
0.050722215324640274,
0.008855942636728287,
0.05937882140278816,
-0.025301627814769745,
0.02430827170610428,
-0.005143404472619295,
-0.014922399073839188,
0.1119605153799057,
-0.004037394188344479,
0.01261946652084589,
0.014852097257971764,
-0.06062127649784088,
0.014288236387073994,
-0.04426658898591995,
0.01631157472729683,
-0.02915460430085659,
-0.06317555159330368,
0.0009730268502607942,
-0.04873501509428024,
0.07398845255374908,
-0.03593536466360092,
-0.03667544201016426,
-0.03170757368206978,
0.007072823122143745,
-0.02946498431265354,
-0.02696470357477665,
-0.008382800035178661,
0.005238020326942205,
0.0676698163151741,
0.007732707541435957,
-0.029894942417740822,
-0.021340105682611465,
-0.017611056566238403,
0.008919226936995983,
0.041021883487701416,
0.005021966528147459,
-0.00716219749301672,
-0.020270857959985733,
0.040681056678295135,
0.031285595148801804
] |
PMID:27104 | Human platelet MAO in drug-free and medicated schizophrenic patients. | The authors compared platelet monoamine oxidase activity in drug-free chronic and acute schizophrenic patients, medicated chronic schizophrenic patients, and normal controls. A significant decrement in MAO activity was found only in medicated chronic schizophrenic patients. The possible mechanism for this finding is discussed. | Human platelet MAO in drug-free and medicated schizophrenic patients. The authors compared platelet monoamine oxidase activity in drug-free chronic and acute schizophrenic patients, medicated chronic schizophrenic patients, and normal controls. A significant decrement in MAO activity was found only in medicated chronic schizophrenic patients. The possible mechanism for this finding is discussed. | [
-0.09894672781229019,
0.03789789229631424,
-0.03473755344748497,
0.020539339631795883,
-0.026296360418200493,
0.015726637095212936,
0.10760648548603058,
0.025997407734394073,
-0.031430114060640335,
-0.06661180406808853,
0.0025177497882395983,
0.023145858198404312,
0.006543072871863842,
0.06236208230257034,
0.01023133099079132,
0.008345083333551884,
0.008240099996328354,
-0.008356702513992786,
-0.056901246309280396,
-0.006832696497440338,
-0.053094640374183655,
-0.06995462626218796,
0.04045248031616211,
0.034955766052007675,
-0.004279900807887316,
0.034389059990644455,
-0.02778014726936817,
-0.04891766980290413,
0.013588367961347103,
-0.10946502536535263,
0.06282367557287216,
-0.007962935604155064,
0.013138004578649998,
-0.022211233153939247,
0.016901561990380287,
0.0058513786643743515,
-0.015705928206443787,
0.06207979470491409,
0.00947052426636219,
0.019798455759882927,
0.052382465451955795,
0.005294555798172951,
0.00654564006254077,
0.06363752484321594,
0.01766163483262062,
0.009411181323230267,
0.004953810945153236,
-0.02066355012357235,
0.05968629568815231,
0.006027074996381998,
0.08026057481765747,
-0.00822286494076252,
0.026170827448368073,
0.07226019352674484,
-0.0013976457994431257,
-0.025934046134352684,
-0.027833934873342514,
0.0042670853435993195,
-0.003331501968204975,
0.029791876673698425,
-0.034898195415735245,
0.05848519504070282,
-0.13682016730308533,
0.04809527471661568,
0.04862457141280174,
-0.015274153091013432,
-0.015573780052363873,
-0.016926990821957588,
0.02602316252887249,
0.01493540033698082,
-0.0628076121211052,
0.053326383233070374,
0.004691220819950104,
0.01210186816751957,
0.05083636939525604,
-0.017553620040416718,
0.012933384627103806,
-0.044734079390764236,
-0.0303561482578516,
-0.03742566332221031,
-0.011402864940464497,
-0.0510476678609848,
-0.0021499760914593935,
-0.06479280441999435,
0.03484661877155304,
0.03624723479151726,
0.02835654281079769,
0.009320241399109364,
-0.03975571691989899,
0.0022288160398602486,
-0.027226965874433517,
-0.0128836864605546,
-0.0032714137341827154,
0.051778241991996765,
-0.062345292419195175,
0.05356806889176369,
0.02112867869436741,
0.014082971028983593,
-0.06626427173614502,
0.38531234860420227,
-0.03871781378984451,
0.030277248471975327,
-0.04407567158341408,
0.003948494326323271,
-0.017081307247281075,
0.00009159164619632065,
-0.014814816415309906,
-0.032901812344789505,
-0.009692434221506119,
0.03813765197992325,
0.013688337057828903,
0.06530752778053284,
0.08385514467954636,
-0.079015351831913,
-0.006568464450538158,
-0.04091855511069298,
0.03412572667002678,
0.021527502685785294,
0.019643545150756836,
0.08882588893175125,
0.008749355562031269,
0.02627009153366089,
0.0025913589634001255,
-0.046518102288246155,
0.007020739372819662,
0.025972461327910423,
0.005407287739217281,
0.11121939867734909,
0.012723761610686779,
-0.050736065953969955,
0.05121046304702759,
-0.0002562071313150227,
0.006616577040404081,
0.021828582510352135,
0.02099272422492504,
0.025285037234425545,
0.03351970762014389,
-0.029589224606752396,
0.09505052119493484,
-0.033932045102119446,
0.006461759563535452,
-0.0029268660582602024,
-0.008811191655695438,
-0.05909361690282822,
-0.06276397407054901,
0.07987596094608307,
0.039439085870981216,
0.001976174768060446,
0.006982876919209957,
0.04012611135840416,
-0.008279681205749512,
0.07143206894397736,
-0.03126572445034981,
0.05512751638889313,
-0.011705676093697548,
0.019440973177552223,
-0.01913473568856716,
-0.018572593107819557,
-0.008585577830672264,
-0.016148963943123817,
-0.05150297284126282,
-0.04586666822433472,
-0.025029446929693222,
0.1636001020669937,
0.04326820746064186,
-0.03731673210859299,
-0.011974851600825787,
-0.06406434625387192,
0.009703991934657097,
0.01165066845715046,
0.005717641673982143,
0.028350133448839188,
-0.07159371674060822,
0.016394883394241333,
-0.04595962539315224,
-0.07989199459552765,
0.019644733518362045,
0.06467397511005402,
0.011417686939239502,
-0.008679740130901337,
0.014342878945171833,
0.0023782921489328146,
-0.058745138347148895,
-0.014904643408954144,
-0.009469290263950825,
-0.012581820599734783,
-0.03213324770331383,
-0.00408140616491437,
0.04144171625375748,
-0.013963581062853336,
-0.045989878475666046,
-0.0007976093329489231,
-0.02908368594944477,
0.029276596382260323,
0.0019016353180631995,
-0.013697445392608643,
-0.06324146687984467,
-0.04737929254770279,
-0.07538486272096634,
-0.003232864197343588,
0.09367965161800385,
0.0011134619126096368,
-0.014367238618433475,
0.004591844510287046,
0.040317412465810776,
0.0011160288704559207,
0.03666076064109802,
-0.01840183697640896,
0.028164464980363846,
0.05133626610040665,
-0.023086072877049446,
-0.00693849939852953,
0.003348134458065033,
0.030676020309329033,
-0.06059896573424339,
0.07633684575557709,
-0.018868830054998398,
-0.03126521781086922,
0.06102515757083893,
0.01728701964020729,
-0.012555379420518875,
-0.021613607183098793,
-0.052212342619895935,
-0.28821778297424316,
-0.03790606930851936,
0.04967905580997467,
-0.034188639372587204,
-0.015248041599988937,
-0.03960360959172249,
-0.022632939741015434,
-0.12558719515800476,
0.009512861259281635,
0.024542393162846565,
0.021225636824965477,
0.024354303255677223,
-0.01954803802073002,
-0.014565887860953808,
0.0043175783939659595,
0.017264148220419884,
0.020501213148236275,
-0.07041070610284805,
-0.03204357624053955,
0.013301526196300983,
0.03730693459510803,
-0.009129893034696579,
0.0033961497247219086,
-0.05702558532357216,
0.036731138825416565,
-0.03483516350388527,
0.14754247665405273,
0.014101983979344368,
-0.011623689904808998,
0.013735810294747353,
-0.0369933620095253,
0.0179948341101408,
0.0031333828810602427,
-0.12080644816160202,
-0.009617218747735023,
-0.06021960452198982,
-0.04189787060022354,
-0.10422632098197937,
-0.012410517781972885,
0.0366993173956871,
-0.05528990924358368,
-0.05415019392967224,
0.06803932040929794,
-0.04653278738260269,
-0.0990443006157875,
-0.0070355613715946674,
0.014969795010983944,
0.04082072526216507,
-0.007124326191842556,
-0.021894142031669617,
0.019954463467001915,
0.0033551256638020277,
0.030843639746308327,
-0.012304309755563736,
-0.024950429797172546,
0.04986882582306862,
-0.07066920399665833,
-0.04230612516403198,
-0.08977380394935608,
-0.012399033643305302,
-0.037089694291353226,
-0.002053217263892293,
0.029074639081954956,
0.00954317208379507,
0.015207871794700623,
0.05063438042998314,
0.04761099815368652,
-0.03192339837551117,
0.08010094612836838,
0.006387299858033657,
0.012521260417997837,
0.12314189225435257,
0.00615862850099802,
0.025811713188886642,
0.04349156469106674,
-0.016153251752257347,
-0.03133552148938179,
-0.025702105835080147,
-0.0009213859448209405,
0.08759325742721558,
0.0018711804877966642,
0.00417140731588006,
0.04596337303519249,
0.07088831812143326,
0.0005269821849651635,
0.003733535995706916,
-0.002800865564495325,
-0.03824922814965248,
-0.014448744244873524,
0.01654062606394291,
0.06267974525690079,
0.04654384031891823,
-0.033589333295822144,
-0.07139115780591965,
0.05423051118850708,
-0.021711044013500214,
-0.2756144404411316,
0.00552329421043396,
0.053753603249788284,
0.021290645003318787,
-0.025969315320253372,
0.02167784981429577,
-0.015200333669781685,
0.013017511926591396,
-0.016953781247138977,
0.01986459270119667,
0.027264734730124474,
0.004337124060839415,
0.0389668233692646,
-0.007568706292659044,
-0.026195786893367767,
-0.008832105435431004,
0.04515605792403221,
0.018621014431118965,
0.01242655236274004,
0.03811689466238022,
0.022416479885578156,
-0.02035440132021904,
0.13343439996242523,
0.015308063477277756,
0.0295886043459177,
0.021209552884101868,
0.008304215036332607,
0.017177196219563484,
-0.06609667092561722,
-0.005412203725427389,
-0.02865668013691902,
0.0194148737937212,
0.02492925152182579,
0.03223970904946327,
0.0640253946185112,
0.004055495839565992,
-0.08029631525278091,
-0.03509055823087692,
0.008817637339234352,
0.0007108550053089857,
-0.06781288981437683,
0.03457268327474594,
-0.060953982174396515,
0.02512398548424244,
0.05784149840474129,
-0.060526296496391296,
-0.07238578051328659,
-0.03912787884473801,
-0.0077367741614580154,
0.02243334800004959,
-0.029980512335896492,
-0.04309345409274101,
-0.027689890936017036,
0.026836764067411423,
0.003237471915781498,
-0.00910774152725935,
-0.02026645839214325,
-0.017230389639735222,
0.05298836529254913,
-0.007462697569280863,
-0.028966592624783516,
0.04725214093923569,
-0.013857515528798103,
0.054742392152547836,
-0.042299795895814896
] |
PMID:27107 | [The importance of age dependent changes of the organism for therapy (author's transl)]. | Molecular and supramolecular mechanisms of ageing involve biochemical, physiological and morphological alterations of organs. Furthermore physiological disturbances are superimposed by pathological mechanisms of ageing. As a result the number of diseases in higher age increases as well as there are a lot of therapeutical problems. The following paper tries to reveal that physiological and pathological changes of different organs (heart, kidney, lung, brain) influence the therapy of older man. The results demonstrate that it is necessary to stimulate the research of pharmacodynamics and kinetics in the higher age. | [The importance of age dependent changes of the organism for therapy (author's transl)]. Molecular and supramolecular mechanisms of ageing involve biochemical, physiological and morphological alterations of organs. Furthermore physiological disturbances are superimposed by pathological mechanisms of ageing. As a result the number of diseases in higher age increases as well as there are a lot of therapeutical problems. The following paper tries to reveal that physiological and pathological changes of different organs (heart, kidney, lung, brain) influence the therapy of older man. The results demonstrate that it is necessary to stimulate the research of pharmacodynamics and kinetics in the higher age. | [
-0.03021841309964657,
-0.01457593496888876,
0.02316218614578247,
0.01772359013557434,
0.0323537178337574,
-0.007920004427433014,
0.07752635329961777,
-0.017120860517024994,
-0.058773718774318695,
-0.012316540814936161,
0.039611779153347015,
-0.010320213623344898,
-0.003008643863722682,
0.0636168122291565,
0.025814708322286606,
0.032253097742795944,
0.030244896188378334,
0.03419271856546402,
-0.04693162441253662,
-0.0013808577787131071,
-0.014345669187605381,
0.027584446594119072,
-0.002873673802241683,
-0.026249287649989128,
-0.007907523773610592,
0.015226977877318859,
0.003876212751492858,
0.016395971179008484,
-0.030179550871253014,
-0.12149564921855927,
0.04998775199055672,
0.03171183541417122,
0.0007286494364961982,
-0.03190486878156662,
0.010964552871882915,
0.021047117188572884,
-0.01493224035948515,
-0.028824636712670326,
-0.0131198326125741,
0.0031784744933247566,
0.03851063549518585,
0.029488058760762215,
-0.02454341948032379,
-0.0029019052162766457,
0.0387723445892334,
0.012220604345202446,
-0.042257051914930344,
-0.0036548497155308723,
0.05544812232255936,
0.008084823377430439,
-0.026421688497066498,
-0.0010535274632275105,
-0.04098524898290634,
0.09792003035545349,
0.028187941759824753,
-0.03516380488872528,
0.030423058196902275,
0.07844135910272598,
0.03925474360585213,
0.027174098417162895,
-0.0053745475597679615,
0.04777219146490097,
-0.11656574159860611,
0.06201156601309776,
0.07189875096082687,
-0.0019009452080354095,
-0.012137637473642826,
-0.0063041802495718,
0.09161532670259476,
0.02023942582309246,
-0.09375191479921341,
0.028606027364730835,
0.03127622976899147,
0.005345284938812256,
0.04218969866633415,
-0.03940143063664436,
-0.04836353287100792,
-0.05599347874522209,
0.03191805258393288,
0.016887065023183823,
0.08674120903015137,
0.09702413529157639,
-0.024524850770831108,
-0.02354530431330204,
-0.04243018478155136,
-0.051067054271698,
0.018668804317712784,
-0.08286674320697784,
-0.09100425988435745,
-0.03899557515978813,
-0.05070744454860687,
-0.012418165802955627,
-0.01398018840700388,
0.030625855550169945,
-0.021350713446736336,
-0.01591503992676735,
-0.0005015377537347376,
-0.016330314800143242,
-0.022024070844054222,
0.3766612112522125,
-0.019242800772190094,
0.03316338360309601,
-0.02822861634194851,
0.061636533588171005,
-0.014714226126670837,
-0.022908810526132584,
-0.0011014112969860435,
-0.03857339546084404,
0.01866612397134304,
0.11320141702890396,
-0.021929750218987465,
0.04392460361123085,
0.01805747114121914,
0.006699221674352884,
0.0003249556466471404,
0.0317726694047451,
0.01803595758974552,
0.07759235054254532,
-0.017980793491005898,
-0.008470672182738781,
-0.018229175359010696,
0.014533587731420994,
0.04854295030236244,
0.03424682095646858,
0.04066389426589012,
-0.006500796880573034,
-0.01753235049545765,
0.08394898474216461,
0.014446987770497799,
-0.03293728828430176,
0.06843270361423492,
0.01786019094288349,
-0.04014620929956436,
0.008850494399666786,
-0.016220081597566605,
-0.024743614718317986,
-0.03240012750029564,
-0.07046351581811905,
0.06106330826878548,
-0.017542174085974693,
-0.05993166193366051,
-0.04918161779642105,
-0.06966731697320938,
-0.05559384077787399,
-0.028560305014252663,
0.13127891719341278,
0.06171906739473343,
0.005430887453258038,
-0.013625194318592548,
0.009302507154643536,
-0.016084518283605576,
0.003598306095227599,
0.004563469905406237,
0.03198123723268509,
-0.058247048407793045,
0.028769582509994507,
-0.01783565990626812,
-0.0449783056974411,
-0.028218859806656837,
-0.06850656121969223,
-0.05355820432305336,
-0.016101164743304253,
-0.018117837607860565,
0.08237211406230927,
0.04450280964374542,
0.008566095493733883,
-0.04452775418758392,
-0.04911042004823685,
0.06986378878355026,
0.0265426617115736,
0.02033919095993042,
-0.01889440231025219,
0.011731667444109917,
0.01248189341276884,
-0.0017099132528528571,
0.007483129855245352,
0.06531783193349838,
0.04122384265065193,
-0.008919207379221916,
0.00928760040551424,
0.07029782235622406,
0.03329620137810707,
-0.026639848947525024,
0.03396010398864746,
0.03565651923418045,
-0.06141236424446106,
-0.044002559036016464,
0.036690760403871536,
0.01966644823551178,
0.024029765278100967,
-0.005567394196987152,
0.035984933376312256,
-0.02860492840409279,
-0.03575274720788002,
-0.02508898451924324,
-0.02292359247803688,
-0.07791297882795334,
0.023257488384842873,
-0.07418673485517502,
0.01439497247338295,
0.04049665480852127,
-0.013125353492796421,
0.007531517185270786,
0.04310647398233414,
-0.03716488182544708,
0.027148369699716568,
0.034014180302619934,
-0.017818083986639977,
0.04321267828345299,
0.01506852824240923,
-0.029081691056489944,
-0.045143693685531616,
0.007351640146225691,
0.038533248007297516,
-0.00823813583701849,
0.07505692541599274,
-0.056272272020578384,
-0.0000463574324385263,
0.04207448288798332,
-0.005384826101362705,
0.051987241953611374,
-0.013033779338002205,
-0.00809339340776205,
-0.30806005001068115,
-0.06145523115992546,
-0.016440993174910545,
-0.027056314051151276,
0.05697927623987198,
-0.04079389572143555,
-0.02636929415166378,
-0.006930395495146513,
-0.02924478054046631,
0.034364379942417145,
0.047533757984638214,
0.045168500393629074,
-0.003129249671474099,
-0.08575503528118134,
-0.018972722813487053,
0.021333923563361168,
0.008743400685489178,
-0.034870948642492294,
-0.04457319527864456,
0.0037288928870111704,
0.04916262626647949,
0.005222267005592585,
0.04835990071296692,
-0.017217202112078667,
0.04183074086904526,
0.0022020158357918262,
0.08365576714277267,
-0.05290897563099861,
-0.10666476935148239,
0.024404827505350113,
0.024831093847751617,
-0.021073905751109123,
-0.03437956050038338,
-0.1007537916302681,
0.04323554039001465,
-0.04711697995662689,
0.031923770904541016,
-0.05170104652643204,
-0.03332867473363876,
-0.06653782725334167,
-0.09407025575637817,
0.0350012332201004,
0.009830359369516373,
0.012049617245793343,
-0.10295288264751434,
-0.023464009165763855,
0.04437775909900665,
-0.01879173330962658,
-0.028362911194562912,
-0.05007860064506531,
0.001301792566664517,
0.06690380722284317,
-0.02788262441754341,
0.014836900867521763,
0.005370771512389183,
-0.04161130264401436,
-0.05782254412770271,
0.03790607303380966,
-0.06310027092695236,
0.004839272703975439,
-0.043215155601501465,
0.054316408932209015,
0.0509067140519619,
-0.03826257586479187,
0.008768454194068909,
-0.05780939385294914,
-0.03255543112754822,
-0.006360917817801237,
0.024335894733667374,
-0.04636015743017197,
0.032418567687273026,
0.11279518902301788,
-0.03990762680768967,
0.02072477713227272,
0.05971796065568924,
0.02491273544728756,
-0.02884860895574093,
0.031185464933514595,
-0.0233981404453516,
0.05458443611860275,
-0.01597471907734871,
-0.0649448037147522,
0.024036046117544174,
0.01081239152699709,
0.016104042530059814,
-0.07799454033374786,
0.0014804223319515586,
-0.005792174953967333,
0.01199620496481657,
0.009876691736280918,
-0.02097558043897152,
-0.03147290647029877,
-0.09515400975942612,
-0.01663980819284916,
0.009742623195052147,
0.022551169618964195,
-0.25443974137306213,
0.034736063331365585,
0.02701261080801487,
0.05007464438676834,
-0.039314720779657364,
-0.014758650213479996,
-0.029555905610322952,
0.015415763482451439,
-0.002675924450159073,
0.0438394695520401,
0.02127874828875065,
0.007131029851734638,
0.0856495350599289,
0.010814188048243523,
-0.006024582777172327,
-0.02033533900976181,
0.07636699080467224,
-0.04295114055275917,
0.0009373799548484385,
-0.03524475172162056,
0.026387859135866165,
0.027949869632720947,
0.143310546875,
-0.031016230583190918,
0.009908054023981094,
0.05569726228713989,
0.016250403597950935,
0.029650907963514328,
-0.027430370450019836,
-0.057883016765117645,
0.0004746649065054953,
0.025461450219154358,
0.038077495992183685,
-0.013432186096906662,
0.02540736459195614,
0.07114560157060623,
-0.023178821429610252,
-0.03031982108950615,
-0.040170542895793915,
0.02248784899711609,
-0.009064893238246441,
0.013525646179914474,
-0.06448549032211304,
-0.04601433128118515,
0.10447364300489426,
-0.02279595099389553,
-0.014671158976852894,
0.01863185130059719,
-0.007712253835052252,
0.0020661226008087397,
-0.05345400795340538,
-0.03499997407197952,
0.05535776540637016,
0.03899320587515831,
-0.00835646316409111,
-0.020989414304494858,
0.013499858789145947,
-0.06834542751312256,
0.03855931758880615,
0.009932477958500385,
-0.009772476740181446,
0.039604220539331436,
-0.076099693775177,
0.10480913519859314,
-0.007864048704504967
] |
PMID:27108 | [Contribution to wet and dry weights of mesenchymal and parenchymatous organs of humans and rats during the course of life (author's transl)]. | The wet and dry weights of parenchymatous (lung, heart, liver, kidney) and of mesenchymal organs (aorta, skin, xiphoid cartilage) of humans and rats (both sexes) were investigated during the course of life. Furthermore, these investigations were done on the following rat organs: brain (divided in cerebrum as well as in cerebellum plus pons), spleen, rib cartilage, testis and ovar. The water content of these tissues and organs of both species was calculated by means of their wet and dry weights. The dry weights of the investigated parenchymal and mesenchymal organs of both species remain constant during the whole course of life (pastly until the high senile age)--using freeze-drying. The same is true for the water content: constancy without significant decrease during the investigated life periods and without sex dependent differences. | [Contribution to wet and dry weights of mesenchymal and parenchymatous organs of humans and rats during the course of life (author's transl)]. The wet and dry weights of parenchymatous (lung, heart, liver, kidney) and of mesenchymal organs (aorta, skin, xiphoid cartilage) of humans and rats (both sexes) were investigated during the course of life. Furthermore, these investigations were done on the following rat organs: brain (divided in cerebrum as well as in cerebellum plus pons), spleen, rib cartilage, testis and ovar. The water content of these tissues and organs of both species was calculated by means of their wet and dry weights. The dry weights of the investigated parenchymal and mesenchymal organs of both species remain constant during the whole course of life (pastly until the high senile age)--using freeze-drying. The same is true for the water content: constancy without significant decrease during the investigated life periods and without sex dependent differences. | [
0.009706830605864525,
-0.032239802181720734,
0.01418273150920868,
-0.02000429853796959,
-0.015405396930873394,
-0.0005272694979794323,
0.048612743616104126,
0.025827068835496902,
-0.027536116540431976,
-0.006336255930364132,
-0.004698496311903,
-0.09235865622758865,
0.03981596231460571,
0.002078395336866379,
-0.0014782218495383859,
0.0010704221203923225,
0.012835606932640076,
0.05430857837200165,
-0.08960357308387756,
0.01965615712106228,
0.025029852986335754,
-0.010717760771512985,
0.026253802701830864,
-0.003362033748999238,
-0.020022017881274223,
-0.029378538951277733,
-0.008040113374590874,
0.0005470675532706082,
-0.00907783955335617,
-0.192729651927948,
0.01140614040195942,
-0.012105446308851242,
0.0346083864569664,
-0.003177911974489689,
0.02480618841946125,
-0.0010709677590057254,
0.003947319928556681,
-0.003675254061818123,
0.010975716635584831,
0.032199449837207794,
0.025250118225812912,
0.057357411831617355,
0.008269645273685455,
0.04273480176925659,
0.03370627760887146,
0.046323493123054504,
-0.025066563859581947,
-0.00463520921766758,
0.0492238849401474,
0.002076301723718643,
-0.005027760285884142,
0.03469540551304817,
0.04537033289670944,
0.11349710822105408,
-0.01312656793743372,
0.012397841550409794,
0.056448813527822495,
0.02337508462369442,
0.03167691081762314,
0.03875284641981125,
-0.032522380352020264,
-0.004298351239413023,
-0.14061515033245087,
0.08321846276521683,
0.06323513388633728,
0.008517776615917683,
-0.057712115347385406,
-0.04307837411761284,
0.012275916524231434,
-0.011113233864307404,
-0.013836697675287724,
0.08065586537122726,
0.10346189141273499,
0.023815874010324478,
0.018762990832328796,
-0.02000592276453972,
-0.028751764446496964,
-0.026934774592518806,
-0.007784643676131964,
0.04014108330011368,
0.01584519073367119,
0.07244440168142319,
0.06132413446903229,
0.011581776663661003,
-0.04525047913193703,
-0.010432759299874306,
-0.041081760078668594,
-0.06363299489021301,
-0.053372424095869064,
-0.021780267357826233,
-0.04071437194943428,
-0.04097575694322586,
-0.05355256050825119,
0.08100965619087219,
-0.05134650692343712,
0.007059948053210974,
-0.0017935282085090876,
-0.0015554634155705571,
0.0037781966384500265,
0.43314164876937866,
-0.0347452238202095,
0.04132075980305672,
-0.009025640785694122,
-0.02468893490731716,
-0.0443367138504982,
0.027599303051829338,
-0.038494646549224854,
-0.044590119272470474,
0.019569868221879005,
0.06495778262615204,
0.0298154279589653,
0.013328331522643566,
-0.029275944456458092,
0.0025705653242766857,
-0.006387736182659864,
-0.07137637585401535,
0.022143827751278877,
0.006337013561278582,
-0.009105297736823559,
0.018732450902462006,
-0.0052664922550320625,
-0.02377748116850853,
0.06848523020744324,
-0.029063493013381958,
0.07153898477554321,
-0.0244122464209795,
0.005312151275575161,
0.07109690457582474,
0.013593249022960663,
0.008905683644115925,
0.061265215277671814,
0.00424965750426054,
-0.011098050512373447,
-0.014768089167773724,
0.012101355008780956,
0.00013806522474624217,
0.02172772027552128,
-0.05064583197236061,
-0.07146335393190384,
-0.025924205780029297,
-0.07437636703252792,
-0.09009768813848495,
0.010259999893605709,
-0.09088747948408127,
-0.09498027712106705,
0.1365225911140442,
0.052644964307546616,
-0.005491955205798149,
-0.001592889428138733,
0.024499917402863503,
-0.05236643925309181,
0.01763804629445076,
0.03960691764950752,
0.01236752886325121,
-0.029235584661364555,
-0.013461214490234852,
-0.036875560879707336,
-0.035510558634996414,
-0.014418012462556362,
-0.05634273961186409,
0.004626621026545763,
-0.02912573516368866,
-0.05355367064476013,
0.05826455354690552,
0.06120378151535988,
0.0037086154334247112,
-0.04927937686443329,
-0.013665239326655865,
0.03811095654964447,
0.002898711245507002,
0.008367519825696945,
-0.060215502977371216,
0.019194345921278,
-0.01938856951892376,
0.024116791784763336,
-0.032988451421260834,
0.023094769567251205,
-0.0023375432938337326,
0.022189799696207047,
0.024742254987359047,
0.041355863213539124,
0.003886366030201316,
-0.04619348421692848,
0.029025493189692497,
0.02261020801961422,
-0.02459663152694702,
-0.053522299975156784,
-0.012694234028458595,
0.029839573428034782,
0.03361177444458008,
0.03333749249577522,
-0.030927790328860283,
-0.051233962178230286,
0.012257520109415054,
-0.048846252262592316,
-0.03372834622859955,
-0.00022369447106029838,
0.023975787684321404,
-0.01808570697903633,
0.006031628232449293,
0.059302736073732376,
-0.054786570370197296,
0.027458975091576576,
0.011962810531258583,
-0.017723437398672104,
0.06303410977125168,
0.056356750428676605,
-0.006473445799201727,
0.04693157225847244,
0.024248238652944565,
-0.05846932530403137,
0.012746640481054783,
-0.04500540718436241,
-0.004356435965746641,
-0.02509644255042076,
0.010154787451028824,
0.0590127632021904,
-0.04392965883016586,
0.019675537943840027,
-0.013699622824788094,
0.06213570013642311,
-0.08831369131803513,
-0.021279193460941315,
-0.27241992950439453,
-0.02802344411611557,
0.011884917505085468,
-0.04987623915076256,
0.1196073591709137,
-0.05131567269563675,
0.025291530415415764,
-0.0448279045522213,
0.018635405227541924,
-0.020247584208846092,
0.03261721134185791,
0.030840877443552017,
-0.024236757308244705,
-0.00011671518586808816,
0.03492892161011696,
0.036191146820783615,
0.07568171620368958,
-0.009604311548173428,
0.01911909691989422,
0.043709807097911835,
0.008664989843964577,
0.004591098055243492,
-0.009947570040822029,
0.0033646232914179564,
0.0835694819688797,
-0.031716279685497284,
0.1397559642791748,
-0.002942829392850399,
-0.05250680446624756,
0.020314890891313553,
-0.0024531895760446787,
-0.0033908970654010773,
-0.005970792844891548,
-0.09143636375665665,
0.014130549505352974,
-0.006361191160976887,
-0.03873750567436218,
-0.005891538690775633,
-0.005436263512820005,
-0.009656297042965889,
-0.06866627931594849,
0.022229179739952087,
0.016969256103038788,
-0.024824585765600204,
-0.04262276366353035,
-0.025396741926670074,
0.015498854219913483,
0.0037779074627906084,
-0.05550040677189827,
-0.04831317067146301,
0.037712328135967255,
-0.02082701586186886,
-0.04102400690317154,
-0.03594975546002388,
-0.02421199157834053,
-0.04178610071539879,
-0.032970208674669266,
-0.027919281274080276,
-0.07705886662006378,
-0.005645437631756067,
0.030119191855192184,
-0.05391590669751167,
0.021436939015984535,
-0.057448554784059525,
-0.012500517070293427,
-0.013810415752232075,
0.00904561672359705,
-0.02787136100232601,
0.0016670426120981574,
-0.037131428718566895,
-0.0024933957029134035,
0.09000112116336823,
-0.010471031069755554,
0.028670500963926315,
0.07042409479618073,
0.016741985455155373,
-0.0408799834549427,
0.025401102378964424,
0.02890770696103573,
0.03502567484974861,
0.02313758246600628,
-0.06335320323705673,
0.0352732315659523,
0.028269901871681213,
0.05013540759682655,
0.02291637286543846,
-0.0019351609516888857,
-0.0242726169526577,
0.03154817223548889,
0.013223896734416485,
0.018253330141305923,
0.0385676734149456,
-0.0652460902929306,
-0.04451371729373932,
0.0015115351416170597,
0.006430808920413256,
-0.29712221026420593,
0.04961065575480461,
0.013806809671223164,
-0.018271105363965034,
0.019583500921726227,
-0.014933357946574688,
-0.01205660030245781,
-0.017094776034355164,
-0.0046286252327263355,
0.0274824108928442,
0.041244469583034515,
0.006925311405211687,
0.03260563313961029,
0.03322077542543411,
-0.0005756689351983368,
0.010793846100568771,
0.03322844207286835,
0.002550112782046199,
0.022110577672719955,
-0.014646484516561031,
-0.020748833194375038,
-0.04423586279153824,
0.12975142896175385,
-0.028531832620501518,
-0.03848106041550636,
0.009576436132192612,
-0.03465374931693077,
0.019109226763248444,
0.02775152400135994,
0.010936112143099308,
0.030682779848575592,
0.04040089249610901,
0.05038633570075035,
-0.0436769463121891,
0.002741198753938079,
0.04772261902689934,
-0.046746257692575455,
0.06120435148477554,
-0.004626603331416845,
-0.006437256466597319,
0.020283609628677368,
-0.029926534742116928,
-0.07290155440568924,
0.01695035584270954,
0.03543936088681221,
-0.008569571189582348,
0.026694560423493385,
-0.014308246783912182,
0.05051913857460022,
0.0012682982487604022,
-0.03527695685625076,
0.009586465544998646,
0.03643029183149338,
-0.00823830347508192,
0.0019097498152405024,
-0.04203984513878822,
0.019912108778953552,
-0.06937029212713242,
0.004886476788669825,
-0.05882997438311577,
0.0054601808078587055,
0.0027376003563404083,
-0.0630873367190361,
0.10552474111318588,
0.019728180021047592
] |
PMID:27109 | [Correlations between age, risk factors for arteriosclerosis and endocrine functions (author's transl)]. | In 377 males and 528 female factory workers basal serum levels of glucose, neutralfat, cholesterol, Na, K, Ca and LH, FSH, TSH, Prolactin, GH, Insulin, total corticoids and testosteron were determined. Mean values and standard deviations as well as correlations between mean basal hormone levels and other serum parameters, blood pressure and relative body weight (Broca Index) and partial correlations excluding age and relative body weight were calculated. Basal serum levels of LH, FSH, GH and prolactin show age and sex dependent secretion patterns. In men serum insulin-, GH- and total corticoid levels correlate positive significant with glucose and cholesterol levels, relative body weight and blood pressure, which possibly shows, that these hormones may play a role in the development of degenerative heart and blood vessel diseases. Testosteron levels and parameters of fat metabolism, relative body weight and diastolic blood pressure correlate negative significant. Obviously testosteron has a protective effect against arterioslcerotic lesions. In women insulin and GH do not have the same importance as in men. Prolactin serum and cholesterol level correlate negative significant. In postmenopausal women LH and FSH serum levels correlate positive significant with glucose and cholesterol levels, blood pressure and relative body weight, the so called risk factors for arteriosclerosis. | [Correlations between age, risk factors for arteriosclerosis and endocrine functions (author's transl)]. In 377 males and 528 female factory workers basal serum levels of glucose, neutralfat, cholesterol, Na, K, Ca and LH, FSH, TSH, Prolactin, GH, Insulin, total corticoids and testosteron were determined. Mean values and standard deviations as well as correlations between mean basal hormone levels and other serum parameters, blood pressure and relative body weight (Broca Index) and partial correlations excluding age and relative body weight were calculated. Basal serum levels of LH, FSH, GH and prolactin show age and sex dependent secretion patterns. In men serum insulin-, GH- and total corticoid levels correlate positive significant with glucose and cholesterol levels, relative body weight and blood pressure, which possibly shows, that these hormones may play a role in the development of degenerative heart and blood vessel diseases. Testosteron levels and parameters of fat metabolism, relative body weight and diastolic blood pressure correlate negative significant. Obviously testosteron has a protective effect against arterioslcerotic lesions. In women insulin and GH do not have the same importance as in men. Prolactin serum and cholesterol level correlate negative significant. In postmenopausal women LH and FSH serum levels correlate positive significant with glucose and cholesterol levels, blood pressure and relative body weight, the so called risk factors for arteriosclerosis. | [
0.014505955390632153,
0.047777436673641205,
-0.012127398513257504,
0.03930355980992317,
0.004496430512517691,
0.0064313896000385284,
0.06050897762179375,
0.02316221222281456,
-0.03413160890340805,
-0.07666991651058197,
-0.005221421364694834,
-0.07735437154769897,
0.03186546638607979,
0.06590237468481064,
-0.06363531947135925,
0.022314749658107758,
0.03420088812708855,
-0.008374480530619621,
-0.0629795715212822,
0.04628372564911842,
-0.015287093818187714,
-0.006860480643808842,
0.036518387496471405,
-0.00946732610464096,
0.008249756880104542,
0.022988347336649895,
-0.021864524111151695,
-0.02212180756032467,
-0.012075603939592838,
-0.13666751980781555,
0.020158477127552032,
-0.03205055370926857,
0.012809457257390022,
-0.035131603479385376,
0.0011523667490109801,
0.011558249592781067,
-0.0032079163938760757,
0.002240580739453435,
0.03480346128344536,
-0.018221093341708183,
-0.0010068558622151613,
-0.03616907820105553,
-0.010029174387454987,
0.027288014069199562,
-0.008562536910176277,
0.040331482887268066,
-0.04213101789355278,
0.027549056336283684,
0.02154042012989521,
0.07489382475614548,
-0.04333561658859253,
-0.013248451985418797,
0.0026865575928241014,
0.0923973023891449,
0.004669010639190674,
-0.020529214292764664,
-0.002581172389909625,
0.009647460654377937,
-0.03203364834189415,
0.023868173360824585,
-0.019467858597636223,
0.03673262894153595,
-0.15051712095737457,
0.05742537975311279,
0.02865000255405903,
0.0012087724171578884,
-0.015460248105227947,
0.0026463547255843878,
0.003325180383399129,
-0.0041177645325660706,
-0.015871884301304817,
0.05932635813951492,
0.04160541296005249,
-0.014138329774141312,
-0.011088123545050621,
0.016996515914797783,
-0.054896280169487,
-0.050595320761203766,
0.014851478859782219,
0.008734420873224735,
0.006048757117241621,
0.0950528085231781,
0.020190291106700897,
0.02644028328359127,
-0.021292686462402344,
-0.029509592801332474,
-0.022095711901783943,
-0.045778725296258926,
0.01972341723740101,
-0.02074326016008854,
-0.061453014612197876,
-0.07054480910301208,
-0.053566329181194305,
0.04477236419916153,
-0.049636706709861755,
0.013776170089840889,
0.0002049433533102274,
-0.058745793998241425,
-0.04796949774026871,
0.3905452787876129,
-0.05199218913912773,
-0.03687474504113197,
-0.028751397505402565,
0.006203943397849798,
0.012475344352424145,
-0.019607147201895714,
-0.003786359680816531,
-0.022346429526805878,
-0.03357545658946037,
0.0610247440636158,
0.01443813368678093,
0.009324182756245136,
0.03771011531352997,
-0.06294971704483032,
-0.026530759409070015,
-0.04791123420000076,
0.053611673414707184,
0.04045591875910759,
-0.04249492660164833,
0.0018616473535075784,
0.008791966363787651,
0.024952538311481476,
0.052453041076660156,
-0.020754998549818993,
0.04336901381611824,
0.010643155314028263,
0.01640774868428707,
0.1052757278084755,
0.05665067583322525,
-0.035820115357637405,
0.020667368546128273,
0.07270948588848114,
-0.05347161740064621,
0.04247758165001869,
0.021266020834445953,
0.007110200822353363,
0.05396127700805664,
0.007858354598283768,
0.017758697271347046,
0.045215047895908356,
-0.01978740654885769,
-0.041076429188251495,
-0.02098347619175911,
-0.03439019247889519,
-0.02011021412909031,
0.13187700510025024,
0.0217436570674181,
0.0057733622379601,
-0.006561564281582832,
0.028207927942276,
0.048086367547512054,
0.04872434586286545,
-0.03406183421611786,
0.03238556161522865,
-0.02501608431339264,
0.03259904310107231,
-0.053389113396406174,
-0.01551700197160244,
-0.012787410989403725,
-0.0030628314707428217,
-0.07495171576738358,
-0.007106679491698742,
-0.041570648550987244,
0.00035976432263851166,
0.067197285592556,
-0.011834181845188141,
-0.018997320905327797,
0.009323002770543098,
-0.02397679165005684,
0.00547243794426322,
0.03517511859536171,
-0.024410590529441833,
-0.04903390631079674,
-0.008344997651875019,
0.02774307318031788,
0.009299129247665405,
0.031613439321517944,
0.02043328993022442,
-0.003723897272720933,
-0.000935650896281004,
0.013716070912778378,
0.0007683318690396845,
-0.016319723799824715,
0.06758897751569748,
0.00559861259534955,
-0.003990445286035538,
-0.07445777952671051,
-0.044013746082782745,
-0.019258618354797363,
0.03488349914550781,
-0.02756410650908947,
-0.01877710409462452,
-0.07764186710119247,
0.00231451028957963,
-0.040101490914821625,
-0.03915543481707573,
-0.03577480465173721,
0.03438888490200043,
-0.017824839800596237,
0.0012610232224687934,
0.05700017511844635,
0.02853364497423172,
0.02620602399110794,
0.02629520744085312,
-0.006346131209284067,
0.030706647783517838,
-0.031509991735219955,
0.004506230354309082,
0.08471522480249405,
0.014285451732575893,
0.013317217119038105,
-0.014286745339632034,
0.040391333401203156,
0.03263508901000023,
-0.04950440675020218,
0.04828348755836487,
0.03020310029387474,
-0.014879705384373665,
0.03532153740525246,
0.018096549436450005,
0.05653661489486694,
-0.02702300436794758,
0.020808588713407516,
-0.323824405670166,
-0.0374026820063591,
0.0021279295906424522,
0.009142253547906876,
-0.01790107972919941,
-0.045374371111392975,
-0.01707950420677662,
0.006489359308034182,
-0.004698683507740498,
0.03201794624328613,
0.030309073626995087,
0.06466173380613327,
-0.07386007905006409,
-0.06616982817649841,
-0.036117780953645706,
0.061692975461483,
0.0144968181848526,
-0.07432737201452255,
-0.01583111844956875,
-0.0034370985813438892,
-0.036897893995046616,
-0.011358569376170635,
0.015073047019541264,
0.028925910592079163,
0.11166985332965851,
-0.015210368670523167,
0.12714289128780365,
-0.04243660718202591,
-0.03909836336970329,
-0.04407725855708122,
0.01114337146282196,
-0.006574959959834814,
-0.001921568182297051,
-0.04772424325346947,
0.04104729741811752,
-0.04479501396417618,
0.006658982019871473,
0.0037425807677209377,
-0.027567459270358086,
0.015900207683444023,
-0.07785125076770782,
-0.00837731547653675,
0.0142895532771945,
0.01275049988180399,
-0.114987812936306,
-0.01798352785408497,
0.014851618558168411,
0.0527149997651577,
-0.019935252144932747,
0.01964002288877964,
0.041983723640441895,
-0.0018300020601600409,
0.04214321821928024,
0.007362435106188059,
0.050136443227529526,
-0.049154188483953476,
-0.1040106862783432,
-0.0016433423152193427,
-0.10124216228723526,
-0.006608352065086365,
0.031241081655025482,
0.019629547372460365,
-0.0016448884271085262,
-0.028898010030388832,
0.0012468015775084496,
-0.00661938451230526,
0.004948788322508335,
0.040293727070093155,
0.017242278903722763,
0.028784077614545822,
0.004584270995110273,
0.0651455968618393,
-0.01826697215437889,
0.022298701107501984,
0.04794953390955925,
-0.007274634204804897,
-0.043735355138778687,
0.03623488172888756,
-0.04970351979136467,
0.023194875568151474,
0.027001038193702698,
-0.06907295435667038,
0.042765554040670395,
0.04158591106534004,
-0.0048334188759326935,
-0.02561982162296772,
0.0058331298641860485,
0.05320402979850769,
0.05406849458813667,
0.02071879804134369,
0.0006989645189605653,
-0.056195568293333054,
-0.029589900746941566,
-0.06770174950361252,
-0.01790843904018402,
0.030615029856562614,
-0.29370322823524475,
-0.045875754207372665,
0.0015626732492819428,
-0.010981031693518162,
0.02657950483262539,
-0.035471197217702866,
-0.021850934252142906,
-0.044550176709890366,
-0.004143708851188421,
0.04589209333062172,
0.03411830961704254,
0.0533420629799366,
0.08447086066007614,
0.006782739423215389,
-0.0665808916091919,
0.04149375110864639,
-0.01277888473123312,
-0.007703961338847876,
0.015540780499577522,
-0.0712498277425766,
0.04346548020839691,
-0.0013562998501583934,
0.13936485350131989,
-0.027905602008104324,
-0.03143029659986496,
0.003670796286314726,
0.04377021640539169,
0.026789803057909012,
0.016143400222063065,
0.043498460203409195,
0.009881136938929558,
0.008180533535778522,
0.05147755518555641,
0.03337825462222099,
0.00679682707414031,
0.1258731633424759,
-0.006759281270205975,
-0.015345052815973759,
0.036866478621959686,
-0.01054509449750185,
0.02370520494878292,
0.007945122197270393,
-0.06560338288545609,
-0.0018245269311591983,
0.0732472762465477,
-0.048690371215343475,
-0.006166013889014721,
-0.0021424852311611176,
0.04337245598435402,
-0.02494524046778679,
-0.02262265793979168,
-0.010711830109357834,
0.026000553742051125,
0.04244041442871094,
-0.022942926734685898,
-0.05386185273528099,
0.00038176163798198104,
-0.08487173914909363,
-0.028682362288236618,
0.01067256461828947,
-0.028675753623247147,
0.04221497103571892,
-0.12648935616016388,
0.10085977613925934,
-0.000988977961242199
] |
PMID:27110 | [Linguistics of ageing, linguistic Gerontology--Why? (author's transl)]. | Intercourse between the young and the old is accompanied by difficulties in finding the right word. Authentic observations will be presented. These every-day experiences have prompted the search for the felicity conditions of verbal interaction between members of different age groups. This concern is called linguistics of ageing (or, etatolinguistics). It is called linguistic gerontology (or, gerontolinguistics) if older people are involved in the verbal interactions to be investigated. Papers from the fields of intelligence psychology, language psychology, and language sociology have indeed reported differences in verbal scores between groups of different ages. What has not been answered as yet, however, is the question whether these differences are, or may be, responsible for the difficulties arising in verbal interaction between different age groups. A review of hypotheses as to why verbal scores differ between different age groups in the way they do will reveal that this problem has not as yet been solved either. In conclusion, a programme for further research will be outlined. | [Linguistics of ageing, linguistic Gerontology--Why? (author's transl)]. Intercourse between the young and the old is accompanied by difficulties in finding the right word. Authentic observations will be presented. These every-day experiences have prompted the search for the felicity conditions of verbal interaction between members of different age groups. This concern is called linguistics of ageing (or, etatolinguistics). It is called linguistic gerontology (or, gerontolinguistics) if older people are involved in the verbal interactions to be investigated. Papers from the fields of intelligence psychology, language psychology, and language sociology have indeed reported differences in verbal scores between groups of different ages. What has not been answered as yet, however, is the question whether these differences are, or may be, responsible for the difficulties arising in verbal interaction between different age groups. A review of hypotheses as to why verbal scores differ between different age groups in the way they do will reveal that this problem has not as yet been solved either. In conclusion, a programme for further research will be outlined. | [
0.015459438785910606,
0.04411015659570694,
0.0018961639143526554,
0.0077646272256970406,
0.0021675287280231714,
-0.0006535025895573199,
0.016280997544527054,
-0.04633582383394241,
-0.041465986520051956,
-0.04751148819923401,
0.00120141776278615,
0.004013754893094301,
0.0004154086927883327,
0.034718550741672516,
-0.004246654454618692,
0.04624016582965851,
-0.03915123641490936,
-0.019039025530219078,
-0.008855839259922504,
0.01662684604525566,
0.03859691321849823,
0.020200807601213455,
0.0054000141099095345,
0.03590269386768341,
0.01985437236726284,
0.0607738122344017,
-0.018963931128382683,
-0.04836580157279968,
0.010310184210538864,
-0.13116727769374847,
0.013089138083159924,
0.052574388682842255,
0.012759492732584476,
-0.0003898009308613837,
-0.06628071516752243,
0.04414837807416916,
-0.0008050242904573679,
0.013132872991263866,
-0.019506128504872322,
0.012667076662182808,
-0.008745158091187477,
-0.03009687177836895,
-0.010901953093707561,
-0.029812725260853767,
-0.014792731031775475,
-0.0314798541367054,
-0.08425475656986237,
0.004385005682706833,
-0.079725481569767,
0.03078204207122326,
-0.039086680859327316,
-0.04457276314496994,
0.025143597275018692,
0.03618299588561058,
-0.02241801656782627,
0.058988023549318314,
0.06831685453653336,
0.04039264842867851,
-0.007151989731937647,
-0.014392604120075703,
0.02519126981496811,
-0.017714429646730423,
-0.14688682556152344,
0.09108240902423859,
-0.0005045123980380595,
-0.009013046510517597,
0.015336030162870884,
-0.006062865257263184,
-0.031012674793601036,
0.008297376334667206,
-0.000475023640319705,
0.007751510478556156,
0.027792299166321754,
0.05361263081431389,
0.04949917644262314,
-0.0034905429929494858,
-0.009446623735129833,
-0.06418550759553909,
0.032407939434051514,
0.018125295639038086,
0.07597837597131729,
0.057943861931562424,
-0.023009464144706726,
-0.024828914552927017,
-0.03371499851346016,
-0.04948572441935539,
-0.041207682341337204,
-0.060148272663354874,
-0.04112720862030983,
-0.013648423366248608,
-0.03491969779133797,
0.024620307609438896,
-0.032571226358413696,
0.033564116805791855,
-0.004219885915517807,
-0.046866729855537415,
0.013854661025106907,
0.04857971519231796,
-0.024069081991910934,
0.4524763822555542,
-0.036344241350889206,
0.03586972877383232,
0.015455804765224457,
-0.018149657174944878,
0.010973069816827774,
-0.012758261524140835,
-0.03976253420114517,
-0.053565483540296555,
0.011419931426644325,
0.05833187326788902,
-0.05978952348232269,
0.023904506117105484,
0.025641877204179764,
0.025582894682884216,
0.008922599256038666,
-0.006432312540709972,
0.07066791504621506,
0.024608982726931572,
0.024196943268179893,
0.004233129322528839,
0.00013292291259858757,
0.02165018953382969,
-0.0013855625875294209,
-0.03264685347676277,
0.038090623915195465,
-0.044889841228723526,
-0.011841155588626862,
0.06230688467621803,
0.0050129154697060585,
-0.08880166709423065,
0.02948145382106304,
-0.010965552181005478,
0.010994596406817436,
0.01919259876012802,
0.012980074621737003,
0.017286643385887146,
-0.02295495942234993,
0.02630302496254444,
0.04592351242899895,
-0.042248502373695374,
-0.023397384211421013,
-0.10714533925056458,
0.01983514241874218,
-0.006880948785692453,
-0.043035510927438736,
0.11194552481174469,
-0.030242426320910454,
-0.0066023897379636765,
0.025068430230021477,
0.09113554656505585,
0.0006381566054187715,
0.01346613373607397,
0.009964585304260254,
-0.018072523176670074,
-0.020270003005862236,
-0.0059966836124658585,
0.03270558640360832,
-0.01638711616396904,
-0.001127040944993496,
-0.051913850009441376,
0.041556067764759064,
-0.04975557699799538,
-0.045359138399362564,
0.03747536242008209,
0.04150054231286049,
-0.054325804114341736,
-0.021345335990190506,
0.021672021597623825,
-0.002323743188753724,
-0.05942351371049881,
0.07483762502670288,
0.011293871328234673,
-0.004673412069678307,
0.043447624891996384,
-0.01708878017961979,
0.024367105215787888,
0.007228800095617771,
0.0013484286610037088,
-0.009998629800975323,
0.04488413408398628,
0.043116793036460876,
0.010133297182619572,
-0.025027049705386162,
0.015875888988375664,
-0.007197821978479624,
-0.06284058839082718,
-0.019270477816462517,
-0.038570817559957504,
0.10613550245761871,
-0.0028848822694271803,
0.005573264788836241,
0.0027731729205697775,
0.022635553032159805,
0.0041101532988250256,
-0.024281226098537445,
0.005880225915461779,
-0.000835307699162513,
-0.015400809235870838,
-0.04666166380047798,
0.013518134132027626,
-0.023761408403515816,
0.026233162730932236,
-0.0022219219245016575,
-0.0038761848118156195,
0.020712973549962044,
0.019773896783590317,
0.061937566846609116,
-0.05565307289361954,
0.08212821185588837,
-0.004960198421031237,
-0.0301500391215086,
-0.08284901082515717,
0.04348813742399216,
0.049936145544052124,
-0.010419799946248531,
0.04432661086320877,
-0.009625007398426533,
0.019754139706492424,
-0.03323885425925255,
0.0381929837167263,
-0.03073406219482422,
-0.012919495813548565,
-0.01635907217860222,
-0.30610910058021545,
-0.04600498080253601,
0.0596446730196476,
-0.044484712183475494,
0.07316739112138748,
-0.03884328156709671,
0.041366200894117355,
-0.001853878959082067,
0.008927571587264538,
0.03303078934550285,
-0.013022080063819885,
0.01672980561852455,
-0.021224867552518845,
-0.013508462347090244,
-0.014287388883531094,
0.008697411976754665,
-0.006067715585231781,
-0.012860814109444618,
-0.005007793195545673,
-0.0031021253671497107,
0.03140926733613014,
-0.018243899568915367,
-0.024061381816864014,
-0.04145068675279617,
0.03382686898112297,
-0.010299562476575375,
0.11968367546796799,
-0.007958932779729366,
-0.037136051803827286,
0.0000060840848163934425,
0.0024532291572541,
-0.003747394774109125,
-0.011417672969400883,
-0.047311585396528244,
0.08124218136072159,
-0.014963600784540176,
-0.021119432523846626,
-0.04511793702840805,
-0.017166193574666977,
-0.06157594919204712,
-0.03414861857891083,
-0.005381238181143999,
0.05277123302221298,
0.004353569354861975,
-0.056337229907512665,
0.03199588879942894,
0.047690510749816895,
-0.06189549341797829,
0.005585638806223869,
-0.009613526985049248,
-0.0066138035617768764,
0.04684915021061897,
0.03424883633852005,
0.043811995536088943,
-0.05730801448225975,
-0.02614763379096985,
-0.09048368781805038,
-0.007967204786837101,
-0.048178840428590775,
-0.0042741973884403706,
0.024460379034280777,
0.06020307168364525,
0.05686699599027634,
-0.03383631631731987,
0.022068176418542862,
-0.038426510989665985,
-0.019613871350884438,
-0.01379208080470562,
-0.01306093204766512,
0.051265086978673935,
0.013206702657043934,
0.11584446579217911,
-0.11391600966453552,
-0.03601713478565216,
-0.038599953055381775,
0.052256014198064804,
-0.03133125603199005,
-0.03137936815619469,
0.02763654850423336,
-0.013319456949830055,
0.05530989170074463,
-0.06704707443714142,
0.05249074846506119,
0.053580597043037415,
0.0016380000161007047,
-0.0875680074095726,
-0.009166543371975422,
-0.00649598240852356,
0.03786611184477806,
0.027911094948649406,
-0.012714876793324947,
0.015046797692775726,
-0.022837193682789803,
-0.058879829943180084,
0.007088175043463707,
0.013029231689870358,
-0.2868386209011078,
0.030567867681384087,
0.004581485874950886,
-0.0044205146841704845,
0.0003612819709815085,
0.0345311239361763,
-0.00918743945658207,
-0.0013592824107035995,
-0.026387890800833702,
0.04245259612798691,
0.04497109353542328,
0.04102073609828949,
0.012458602897822857,
0.016996610909700394,
-0.047814592719078064,
0.014364444650709629,
0.09855828434228897,
-0.005011591594666243,
-0.022826755419373512,
-0.009157813154160976,
0.04906199872493744,
0.018909137696027756,
0.14940187335014343,
0.0052969916723668575,
0.0396212637424469,
-0.0160681139677763,
0.07008817046880722,
0.010980939492583275,
0.018805596977472305,
-0.07841215282678604,
-0.05826129391789436,
0.017649391666054726,
0.10229890048503876,
-0.009321892634034157,
0.024047181010246277,
0.026759572327136993,
-0.021068671718239784,
-0.07916808128356934,
0.04590461030602455,
-0.00245351018384099,
-0.03544711694121361,
0.05314936116337776,
-0.04091239348053932,
-0.0462268590927124,
0.06731533259153366,
0.03778994455933571,
0.0068463971838355064,
-0.02361154742538929,
-0.0004262903821654618,
-0.03145598620176315,
-0.03455811366438866,
-0.040554482489824295,
-0.001351060695014894,
0.020375799387693405,
0.04990418627858162,
-0.024715952575206757,
0.011312447488307953,
-0.05280023068189621,
0.01805577613413334,
-0.008239904418587685,
-0.07347964495420456,
0.013996362686157227,
0.021634576842188835,
0.030846161767840385,
-0.0402987003326416
] |
PMID:27111 | [On the social situation of sick old women (author's transl)]. | By explaining the social situation of the patients in a Berlin hospital for chronic and geriatric diseases, it is demonstrated that financial security and social status of old woman patients are much worse than those of old men. Examples are mentioned to demonstrate which were--and still are--the main causes--that in their earlier years women were paid less for their work and were discriminated because of their sex. If for the future the situation of old women is to be improved, the emancipation of women must advance today. | [On the social situation of sick old women (author's transl)]. By explaining the social situation of the patients in a Berlin hospital for chronic and geriatric diseases, it is demonstrated that financial security and social status of old woman patients are much worse than those of old men. Examples are mentioned to demonstrate which were--and still are--the main causes--that in their earlier years women were paid less for their work and were discriminated because of their sex. If for the future the situation of old women is to be improved, the emancipation of women must advance today. | [
0.012585875578224659,
0.05847417563199997,
-0.05004854127764702,
0.013938607648015022,
0.0615360289812088,
0.03745555877685547,
0.037987980991601944,
0.019360190257430077,
-0.028093881905078888,
-0.022529086098074913,
-0.02067572809755802,
0.004315382335335016,
-0.024965861812233925,
-0.002628159476444125,
-0.04945390298962593,
0.011469684541225433,
-0.049710117280483246,
0.013778017833828926,
-0.080265112221241,
0.12129586189985275,
-0.04825045168399811,
-0.032430484890937805,
-0.014534085057675838,
0.0038899220526218414,
0.04880010709166527,
-0.02529068849980831,
-0.027163086459040642,
-0.04830847680568695,
-0.04289699345827103,
-0.08519025146961212,
-0.08889664709568024,
0.0115131177008152,
0.036456186324357986,
-0.02287738211452961,
0.06660547107458115,
0.005336189176887274,
0.054257094860076904,
0.04822397977113724,
-0.05331554263830185,
-0.023870760574936867,
-0.02513982728123665,
-0.009621367789804935,
-0.006495676934719086,
-0.05445689335465431,
-0.008013490587472916,
0.0172418225556612,
0.002925628563389182,
0.0458691380918026,
-0.07789616286754608,
-0.011246736161410809,
-0.024244919419288635,
0.00012117035657865927,
-0.026309551671147346,
0.07956861704587936,
0.06348396092653275,
-0.03716488927602768,
0.03235878795385361,
0.0447680801153183,
-0.03937830030918121,
-0.0012053478276357055,
0.013498586602509022,
-0.04345244541764259,
-0.15087033808231354,
0.012952483259141445,
0.04900757223367691,
0.0058909752406179905,
0.021187080070376396,
-0.005568403750658035,
0.039571814239025116,
-0.024910908192396164,
-0.03871515765786171,
0.015381141565740108,
0.010296789929270744,
0.031054813414812088,
0.06409059464931488,
0.01358821988105774,
-0.0024874540977180004,
-0.044616542756557465,
-0.010663949884474277,
0.011434316635131836,
0.049855172634124756,
0.06785064935684204,
0.04652455449104309,
0.02045237459242344,
-0.044920094311237335,
-0.06314209848642349,
-0.014315123669803143,
-0.06692387163639069,
0.0006940740859135985,
-0.010048816911876202,
-0.02363211289048195,
0.04021843895316124,
0.025136549025774002,
0.030636167153716087,
0.030684959143400192,
-0.030478715896606445,
-0.012931153178215027,
0.04276184365153313,
-0.026303734630346298,
0.4369155466556549,
0.010917452163994312,
0.05503362789750099,
0.030951399356126785,
-0.04600924253463745,
0.047210149466991425,
-0.006287567783147097,
0.006519082933664322,
-0.03345264121890068,
0.009167364798486233,
0.010504227131605148,
-0.05638980492949486,
0.00463748537003994,
0.028583481907844543,
0.02877444587647915,
-0.01698676310479641,
-0.021777275949716568,
0.07395770400762558,
0.0670267716050148,
0.017816780135035515,
0.009324734099209309,
0.01282561756670475,
-0.027866676449775696,
-0.00336580746807158,
-0.006454829592257738,
0.02952400967478752,
-0.045465320348739624,
-0.01554644014686346,
0.08913560956716537,
-0.02844449132680893,
-0.03897148743271828,
0.02790798433125019,
-0.040231723338365555,
-0.0015861812280490994,
0.0682729035615921,
-0.017311668023467064,
-0.03346885368227959,
-0.05529089644551277,
0.05081114172935486,
-0.019257232546806335,
-0.08530306816101074,
0.027122406288981438,
-0.1349644511938095,
-0.06056445091962814,
-0.027940766885876656,
-0.03730456531047821,
0.0827576071023941,
0.023672975599765778,
-0.013059512712061405,
0.05948210135102272,
0.03066170960664749,
0.029889825731515884,
0.03476519137620926,
-0.0016206441214308143,
-0.017191627994179726,
-0.002191019244492054,
0.025385675951838493,
0.014583496376872063,
-0.0044213635846972466,
-0.052671048790216446,
-0.051247864961624146,
0.06177065148949623,
-0.06220177561044693,
-0.05834092199802399,
0.05935617536306381,
0.03491009771823883,
-0.01159300934523344,
-0.032494354993104935,
-0.025546452030539513,
0.02421155758202076,
0.011779610998928547,
0.060289692133665085,
-0.022635044530034065,
0.01804237626492977,
0.04318099468946457,
0.07240381091833115,
0.02409321628510952,
0.04783077910542488,
0.007630049251019955,
-0.04937442019581795,
0.007726933341473341,
0.006593734957277775,
0.018633147701621056,
-0.05174000561237335,
0.038119226694107056,
0.011289192363619804,
-0.03879614546895027,
-0.010786202736198902,
0.042025160044431686,
0.03279096260666847,
0.0009198392508551478,
0.02145722135901451,
0.03796475753188133,
-0.017500679939985275,
-0.022326303645968437,
0.016862593591213226,
-0.031978074461221695,
-0.03974940627813339,
-0.05216708779335022,
-0.0061402395367622375,
0.030068248510360718,
-0.04096858203411102,
0.005317578092217445,
0.015952758491039276,
-0.011145255528390408,
-0.006077971309423447,
-0.012025563977658749,
-0.0015091183595359325,
-0.11347652971744537,
0.07953960448503494,
0.0047134882770478725,
-0.021063970401883125,
-0.012880397029221058,
0.09682535380125046,
0.03455263376235962,
0.05922224000096321,
0.044230539351701736,
0.05794854834675789,
-0.062012992799282074,
0.07236660271883011,
0.01665348932147026,
0.08035854995250702,
0.02008799836039543,
-0.022927602753043175,
-0.32274672389030457,
0.003459663363173604,
-0.025367125868797302,
-0.07551437616348267,
-0.011681207455694675,
-0.0009530509705655277,
0.018641086295247078,
-0.028139395639300346,
-0.0313318707048893,
0.02518782950937748,
0.07423989474773407,
0.016138525679707527,
-0.018845709040760994,
-0.03349921107292175,
0.012979620136320591,
-0.008563016541302204,
0.004959608893841505,
-0.011522527784109116,
-0.04447983205318451,
-0.027384653687477112,
-0.0043188463896512985,
-0.02875465713441372,
0.05765831470489502,
0.007843850180506706,
0.0752616673707962,
-0.04003065824508667,
0.1047908365726471,
-0.041623372584581375,
-0.020979901775717735,
-0.01998589187860489,
-0.003122706199064851,
-0.0048933932557702065,
-0.023479420691728592,
-0.06286608427762985,
0.058175068348646164,
-0.014833198860287666,
0.002809489145874977,
-0.04025634005665779,
-0.045011524111032486,
0.02353779785335064,
-0.047364309430122375,
-0.019176574423909187,
-0.015567251481115818,
0.04786435142159462,
-0.10910104960203171,
0.03911839425563812,
0.031373973935842514,
0.012810774147510529,
-0.007465395610779524,
0.04025961831212044,
-0.032568059861660004,
0.010356727987527847,
-0.02040114440023899,
0.06483432650566101,
0.011511041782796383,
-0.005905363708734512,
-0.056994132697582245,
0.03572830557823181,
-0.07843662053346634,
0.046320125460624695,
-0.009199533611536026,
0.024016544222831726,
0.03188838064670563,
-0.036579787731170654,
0.024330299347639084,
-0.04577532783150673,
-0.048606596887111664,
-0.016108442097902298,
-0.020130572840571404,
0.002233956241980195,
0.04309024289250374,
0.10335484147071838,
-0.023170553147792816,
-0.04611628130078316,
0.02733289822936058,
0.029256746172904968,
0.007466123905032873,
0.007489941082894802,
-0.01940940134227276,
0.01047484576702118,
0.03151649236679077,
-0.03973352164030075,
0.033525701612234116,
0.07497141510248184,
0.010628437623381615,
-0.04056880250573158,
0.025506457313895226,
0.014411103911697865,
0.02421233244240284,
0.03669627010822296,
-0.09705186635255814,
-0.011012344621121883,
-0.06977477669715881,
-0.01926962099969387,
-0.0029807081446051598,
-0.03589044511318207,
-0.2634198069572449,
0.021784743294119835,
-0.019865261390805244,
-0.0004768493236042559,
-0.06809744238853455,
0.01510548871010542,
-0.08362375199794769,
-0.004024541936814785,
-0.00683483574539423,
0.014163091778755188,
0.0562509261071682,
0.0400986410677433,
0.04905489832162857,
-0.02855573780834675,
0.022665975615382195,
-0.007308400236070156,
-0.04974156618118286,
-0.015015953220427036,
-0.018184112384915352,
-0.04950015991926193,
0.030227266252040863,
-0.009583516977727413,
0.08561352640390396,
-0.021281061694025993,
-0.01833496056497097,
0.009050576947629452,
0.019647827371954918,
0.015085143968462944,
0.03722217306494713,
0.03805381804704666,
-0.026172177866101265,
0.016821911558508873,
0.05691985413432121,
0.03342869505286217,
0.0005420076777227223,
-0.008786173537373543,
-0.0094031672924757,
0.013967215083539486,
0.026349879801273346,
0.03411075100302696,
-0.05463777855038643,
0.026637237519025803,
-0.023077702149748802,
0.015591996721923351,
0.09884140640497208,
-0.030316315591335297,
0.01304903719574213,
-0.011019961908459663,
0.02716583013534546,
0.03151353448629379,
0.002413962734863162,
-0.021094834432005882,
0.03348242864012718,
0.04936005175113678,
0.0276369359344244,
-0.014181968756020069,
-0.05577808618545532,
0.0042726388201117516,
0.026694485917687416,
-0.026015527546405792,
-0.05027215555310249,
-0.00346735049970448,
-0.05699313059449196,
0.027206821367144585,
-0.011206177994608879
] |
PMID:27112 | [Analyses of space available in homes and day centers for the aged, as well as nursing homes for elderly people, in the FRG. Booking and occupation rate in homes and nursing homes for the aged (author's transl)]. | Further intention of the survey having been conducted to show the consequences of the "Heimmindestverordnung" (draft dated Nov. 1974) was to obtain a review of the density of occupancy related to housing for the elderly. In homes for the old the rate of one-bed-rooms significantly increased during the last 15 years, whereas the respective quota for rooms with two, three, or more beds showed a decreasing tendency. In nursing homes density also decreased: the rate of one- and two-bed-rooms increased and the respective frequency of rooms equipped with four and more beds decreased from 55% to 13%. Regarding the density of occupancy also the other data collected such as related to variables as association the investigation-unit belongs to, commencement of operation, federal state, size of community led to some striking findings. | [Analyses of space available in homes and day centers for the aged, as well as nursing homes for elderly people, in the FRG. Booking and occupation rate in homes and nursing homes for the aged (author's transl)]. Further intention of the survey having been conducted to show the consequences of the "Heimmindestverordnung" (draft dated Nov. 1974) was to obtain a review of the density of occupancy related to housing for the elderly. In homes for the old the rate of one-bed-rooms significantly increased during the last 15 years, whereas the respective quota for rooms with two, three, or more beds showed a decreasing tendency. In nursing homes density also decreased: the rate of one- and two-bed-rooms increased and the respective frequency of rooms equipped with four and more beds decreased from 55% to 13%. Regarding the density of occupancy also the other data collected such as related to variables as association the investigation-unit belongs to, commencement of operation, federal state, size of community led to some striking findings. | [
0.007655396591871977,
-0.024964239448308945,
0.015042349696159363,
-0.02146381326019764,
0.05986219644546509,
0.06286579370498657,
-0.0007872076821513474,
-0.02654011733829975,
-0.008809041231870651,
-0.0022769575007259846,
0.002359835896641016,
0.02253866381943226,
-0.02539972960948944,
0.07572630792856216,
0.017626386135816574,
0.01601637899875641,
-0.027222132310271263,
-0.0031237881630659103,
-0.0041812327690422535,
0.02276236191391945,
-0.027788162231445312,
-0.02325543388724327,
0.018351752310991287,
-0.0041965763084590435,
0.019095003604888916,
-0.016153447329998016,
-0.03431200608611107,
-0.07735589146614075,
-0.0508517287671566,
-0.13243353366851807,
-0.015431066043674946,
0.013156234286725521,
0.07301153987646103,
-0.032963190227746964,
0.01756284385919571,
0.042081914842128754,
0.05622153729200363,
0.019243739545345306,
-0.005181607790291309,
-0.013171175494790077,
-0.0025607929565012455,
0.034927595406770706,
0.010490883141756058,
-0.028599413111805916,
-0.03299493342638016,
0.041753947734832764,
-0.013967905193567276,
-0.027313729748129845,
-0.018564434722065926,
0.037857670336961746,
-0.009590708650648594,
-0.030149629339575768,
0.0009508557850494981,
0.08665260672569275,
0.049971699714660645,
-0.03308543935418129,
-0.01846030354499817,
0.01500731986016035,
-0.015594948083162308,
0.03665217012166977,
0.045817818492650986,
-0.0013560970546677709,
-0.1960870921611786,
-0.01972123421728611,
0.052051763981580734,
-0.02324172481894493,
-0.03540720418095589,
-0.03732672706246376,
0.05406828597187996,
0.02701045386493206,
-0.014273584820330143,
0.03180570527911186,
-0.018000129610300064,
0.010390397161245346,
0.0924099013209343,
-0.03705894574522972,
-0.005201768130064011,
0.013193516060709953,
0.04922768101096153,
-0.012589769437909126,
0.04718192294239998,
0.0665668472647667,
-0.021268028765916824,
0.012899539433419704,
-0.04473290964961052,
-0.05044149234890938,
-0.030790649354457855,
-0.009549859911203384,
-0.0037695164792239666,
-0.03687033802270889,
0.013664285652339458,
0.024421216920018196,
-0.017844395712018013,
0.03035076893866062,
0.012007104232907295,
-0.04828151687979698,
0.010453990660607815,
0.05518277734518051,
0.020526008680462837,
0.429180771112442,
0.01901220716536045,
0.02409440465271473,
0.038239482790231705,
-0.005309228785336018,
-0.0138193778693676,
-0.058129992336034775,
0.06523143500089645,
-0.0007816379657015204,
0.002051027724519372,
0.0601530447602272,
-0.041320424526929855,
0.0018525728955864906,
0.011335820890963078,
0.003691237885504961,
0.03140630945563316,
0.042040761560201645,
0.044033363461494446,
0.07066527754068375,
-0.0014548145700246096,
0.010855565778911114,
-0.029594236984848976,
-0.034350305795669556,
-0.00258060940541327,
-0.00903897825628519,
0.043767254799604416,
-0.022351810708642006,
-0.01988063007593155,
0.10055892169475555,
0.02373412810266018,
-0.03125254064798355,
-0.029045386239886284,
-0.05589143931865692,
-0.012978429906070232,
-0.017431296408176422,
-0.023040656000375748,
0.047252435237169266,
0.0700034499168396,
0.002838605083525181,
0.07233787328004837,
0.002277732128277421,
0.024410095065832138,
-0.0505976676940918,
-0.016936451196670532,
-0.08116486668586731,
-0.03751596063375473,
0.1348315328359604,
0.03947920352220535,
0.005765197332948446,
0.007968450896441936,
0.019800443202257156,
0.0428791381418705,
0.0714568942785263,
0.013060400262475014,
-0.051937926560640335,
-0.018398821353912354,
0.02228354662656784,
0.0169153343886137,
0.039011452347040176,
-0.00019360527221579105,
-0.01586274430155754,
0.012660113163292408,
-0.0008559281704947352,
-0.02143157087266445,
0.07515762746334076,
-0.01787145808339119,
-0.037533409893512726,
-0.09707193821668625,
0.015396458096802235,
0.006176266819238663,
0.011435671709477901,
0.04914003610610962,
0.028665484860539436,
-0.034062910825014114,
0.04550546035170555,
0.08602645993232727,
0.01447274163365364,
0.022051362320780754,
0.002215153072029352,
-0.024118740111589432,
0.005994797684252262,
-0.04828643426299095,
0.03259431570768356,
-0.045887768268585205,
0.0001416044688085094,
-0.022450145334005356,
-0.0164142195135355,
-0.026320267468690872,
0.027473771944642067,
0.04675900936126709,
-0.00975958351045847,
-0.06055261939764023,
-0.03149697184562683,
-0.022166026756167412,
0.01728326641023159,
-0.0161720123142004,
-0.004746792837977409,
-0.027614090591669083,
0.046297214925289154,
0.03725016862154007,
-0.031210483983159065,
0.050718896090984344,
-0.004823845811188221,
-0.016309430822730064,
0.00797219667583704,
0.025416484102606773,
0.032636694610118866,
0.050777655094861984,
-0.021614717319607735,
0.03749280795454979,
-0.011848237365484238,
0.011622294783592224,
-0.04312560707330704,
0.042688556015491486,
-0.021745769307017326,
0.040059249848127365,
0.05368758738040924,
-0.05259052291512489,
-0.06661783903837204,
0.05115469917654991,
-0.021952012553811073,
0.05460326001048088,
-0.004215619992464781,
-0.032556816935539246,
-0.3434945046901703,
-0.044993262737989426,
0.010885116644203663,
-0.08202149718999863,
-0.010852999053895473,
-0.020673125982284546,
0.0037545652594417334,
0.025460459291934967,
-0.06605047732591629,
-0.0017791046993806958,
0.022116877138614655,
-0.0034885257482528687,
-0.038216378539800644,
0.048822131007909775,
0.03183698281645775,
-0.04792694374918938,
0.02923930436372757,
0.016853872686624527,
-0.08278185874223709,
-0.09454303234815598,
0.027434442192316055,
0.06661316752433777,
-0.028042566031217575,
0.028086507692933083,
0.04768594354391098,
-0.03599518910050392,
0.10026349872350693,
-0.1346004158258438,
-0.012577483430504799,
-0.016419673338532448,
0.033763717859983444,
-0.020663460716605186,
-0.00625945720821619,
-0.050118476152420044,
0.061170633882284164,
0.04073715955018997,
0.011019703932106495,
0.005996339954435825,
-0.017738107591867447,
-0.03969800844788551,
-0.10485128313302994,
0.043831635266542435,
0.0019323209999129176,
-0.016052354127168655,
-0.07882030308246613,
-0.0011791570577770472,
0.01347872894257307,
-0.001864447258412838,
-0.039202433079481125,
-0.01778506115078926,
-0.03583807125687599,
-0.05773761868476868,
0.026690488681197166,
0.029424170032143593,
0.007172078359872103,
-0.03877267241477966,
-0.05181445553898811,
-0.02649654634296894,
-0.051579494029283524,
0.05738300085067749,
-0.004539187066257,
-0.01093444973230362,
0.091775082051754,
-0.039370227605104446,
0.013380439020693302,
-0.06790819019079208,
-0.024839552119374275,
0.030326802283525467,
-0.04122424125671387,
0.008940870873630047,
0.002471173880621791,
0.06464290618896484,
-0.05437704548239708,
-0.039898864924907684,
0.022483179345726967,
0.02238621935248375,
-0.0326426699757576,
0.0451086200773716,
-0.023746877908706665,
-0.018281659111380577,
0.007432455196976662,
-0.07478810101747513,
0.005017989780753851,
0.016253231093287468,
0.017040623351931572,
-0.008398393169045448,
-0.04800952970981598,
0.009341555647552013,
0.011612302623689175,
-0.007977691479027271,
-0.014157888479530811,
0.02782629430294037,
-0.06360271573066711,
-0.009831576608121395,
0.01898297108709812,
0.04475104808807373,
-0.287365585565567,
0.038096025586128235,
-0.01951240375638008,
-0.004379064776003361,
0.024336939677596092,
0.01678948476910591,
-0.026611462235450745,
0.03389488905668259,
0.029791582375764847,
-0.009212059900164604,
0.02835787832736969,
0.07861514389514923,
0.013898443430662155,
-0.010135050863027573,
0.035361334681510925,
0.0032768086530268192,
-0.01017862930893898,
-0.0046907146461308,
0.005754481069743633,
-0.062120236456394196,
0.01158896554261446,
0.04591486603021622,
0.11521542072296143,
-0.005328117869794369,
-0.03568292036652565,
-0.0005299575859680772,
0.043016720563173294,
0.0554337315261364,
0.00198762072250247,
-0.048568204045295715,
-0.045825500041246414,
-0.0037306766025722027,
0.07973834872245789,
-0.032871048897504807,
-0.017106918618083,
-0.01286308839917183,
-0.01462184451520443,
-0.0021692831069231033,
0.012028632685542107,
0.01087829191237688,
-0.03468283265829086,
-0.021380864083766937,
-0.00568972434848547,
0.011686095036566257,
0.09670352190732956,
-0.013383302837610245,
-0.0018667951226234436,
0.0036588613875210285,
0.0031947598326951265,
0.013423322699964046,
-0.03844873979687691,
-0.04221447929739952,
-0.0018133443081751466,
0.009815881960093975,
0.06062348559498787,
-0.006015022750943899,
-0.018118031322956085,
0.028979595750570297,
0.01094771921634674,
-0.05380851402878761,
-0.04172038659453392,
-0.007758093532174826,
0.018377821892499924,
-0.0322817824780941,
0.011668411083519459
] |
PMID:27113 | [Consumer information activities in old age (author's transl)]. | The following feature occupies with consumer information activities in old age. These informations are partial results of a study that was carried out in the Institut für Sozialforschung und Gesellschaftspolitik in Cologne by order of the Arbeitsgemeinschaft der Verbraucher and which deals with chances of consumer policy for people in old age (Naegele 1977). The paper's major elements are, first, essential sources of information of old people (source of supply itself, communication with relatives and friends before buying, written informations), and second, favourite sources of consumer informations to protect and to acquaint old consumers. | [Consumer information activities in old age (author's transl)]. The following feature occupies with consumer information activities in old age. These informations are partial results of a study that was carried out in the Institut für Sozialforschung und Gesellschaftspolitik in Cologne by order of the Arbeitsgemeinschaft der Verbraucher and which deals with chances of consumer policy for people in old age (Naegele 1977). The paper's major elements are, first, essential sources of information of old people (source of supply itself, communication with relatives and friends before buying, written informations), and second, favourite sources of consumer informations to protect and to acquaint old consumers. | [
0.011744201183319092,
0.039223454892635345,
-0.023398734629154205,
-0.027593309059739113,
0.06749579310417175,
0.050377726554870605,
0.03949832543730736,
0.03581241890788078,
-0.04633305221796036,
-0.03215201571583748,
-0.05369846150279045,
0.025672124698758125,
-0.02085610292851925,
0.007012225687503815,
-0.018370117992162704,
0.028098229318857193,
0.047021061182022095,
-0.05290576070547104,
0.009433493949472904,
0.05256377533078194,
0.028104782104492188,
-0.04829704761505127,
0.0038872752338647842,
0.0038182002026587725,
0.051078613847494125,
0.030257485806941986,
-0.00909768883138895,
-0.014565309509634972,
-0.05432622879743576,
-0.07135656476020813,
-0.01123179029673338,
0.013172383420169353,
0.03532613068819046,
0.03367168828845024,
-0.011872846633195877,
-0.030893094837665558,
0.03714313730597496,
-0.012073442339897156,
-0.04930498078465462,
-0.037597816437482834,
-0.018695786595344543,
0.00022609232109971344,
-0.013740606606006622,
-0.05564326047897339,
0.012763864360749722,
-0.009543751366436481,
-0.010895522311329842,
0.0029671473894268274,
-0.02860872633755207,
0.006547951605170965,
-0.056415215134620667,
0.0542609728872776,
0.01873910427093506,
0.011027692817151546,
0.015349134802818298,
0.012850589118897915,
0.008869514800608158,
0.017088914290070534,
-0.005184286739677191,
0.026448996737599373,
0.021759670227766037,
-0.033234160393476486,
-0.17522887885570526,
0.06482837349176407,
-0.04399803653359413,
0.03742215409874916,
-0.03561532124876976,
0.043885525315999985,
-0.009050616063177586,
-0.02498992159962654,
-0.03087281994521618,
0.0030740718357264996,
-0.0033170832321047783,
0.062006350606679916,
0.05211253836750984,
-0.032301682978868484,
0.005118867848068476,
-0.024192780256271362,
-0.03773266449570656,
-0.014044379815459251,
0.04687889665365219,
0.08988694101572037,
-0.011275088414549828,
0.007963157258927822,
-0.04626474902033806,
-0.047001466155052185,
0.029893681406974792,
-0.03458074480295181,
0.03614211827516556,
-0.03339817374944687,
-0.01305354293435812,
0.04801013693213463,
-0.0347398966550827,
0.026118898764252663,
-0.0024941745214164257,
-0.05859433487057686,
0.02925220876932144,
0.055753614753484726,
0.00023372878786176443,
0.44417762756347656,
-0.018380645662546158,
0.04826553538441658,
0.08299867808818817,
-0.032497115433216095,
-0.00010590302554192021,
-0.03407194837927818,
-0.0257260762155056,
0.06083616241812706,
0.013569055125117302,
0.04108298569917679,
-0.0694253221154213,
0.0013201324036344886,
0.035698313266038895,
-0.0011947460006922483,
0.044476017355918884,
0.05778054520487785,
0.032369304448366165,
0.02937278337776661,
0.04629071429371834,
-0.034321028739213943,
-0.028948670253157616,
0.016495123505592346,
0.03224373981356621,
0.045297589153051376,
0.0073417616076767445,
-0.05067722871899605,
-0.006162272300571203,
0.060721591114997864,
-0.03361736983060837,
0.041412077844142914,
0.0065817199647426605,
-0.03608579933643341,
-0.057933561503887177,
0.027812212705612183,
0.01234710868448019,
0.001958372537046671,
-0.048513323068618774,
0.06908125430345535,
0.0691247284412384,
-0.004663395695388317,
-0.021372877061367035,
-0.05165760964155197,
-0.019439836964011192,
-0.020101306959986687,
-0.04005908966064453,
0.08035413920879364,
0.11421540379524231,
0.03350347280502319,
0.010015517473220825,
0.008494623005390167,
0.08851826936006546,
0.06851860880851746,
0.03998218849301338,
-0.04153239354491234,
-0.018100781366229057,
-0.02587456814944744,
0.0547049455344677,
-0.004827800206840038,
-0.028017330914735794,
-0.035020049661397934,
0.02579472027719021,
-0.060724738985300064,
-0.03464478626847267,
0.09397581219673157,
-0.02475081756711006,
-0.07448956370353699,
-0.03329864889383316,
-0.0013559141661971807,
0.038760095834732056,
0.001048880978487432,
0.06596482545137405,
0.0039480277337133884,
-0.017817532643675804,
0.017445966601371765,
0.04977700486779213,
0.03688858449459076,
-0.024946918711066246,
0.009482061490416527,
-0.05060429871082306,
0.004638951271772385,
-0.02836158312857151,
-0.0047265333123505116,
-0.039459917694330215,
-0.020642632618546486,
-0.024005940183997154,
-0.003619502065703273,
-0.027639927342534065,
0.03488793224096298,
0.06364569067955017,
0.028275957331061363,
-0.021049458533525467,
-0.020747110247612,
0.0029600125271826982,
-0.000540588516741991,
-0.013350876979529858,
-0.0046994686126708984,
-0.09671059250831604,
0.05903268977999687,
0.0016419780440628529,
-0.020173171535134315,
-0.02366434410214424,
-0.0718742087483406,
-0.07726384699344635,
-0.0110736433416605,
-0.0038293986581265926,
0.041042760014534,
0.005005501210689545,
-0.06388606876134872,
0.12794367969036102,
-0.0789036676287651,
0.01791233755648136,
0.0011489092139527202,
0.09445660561323166,
0.05875304713845253,
0.02902817726135254,
-0.002853709738701582,
0.0002660898899193853,
-0.015722423791885376,
0.01321590505540371,
0.004751759115606546,
0.03243326023221016,
0.0090998699888587,
-0.03420524299144745,
-0.31437772512435913,
-0.0009265603148378432,
-0.02856222540140152,
-0.03281242400407791,
-0.00011936155351577327,
-0.017111539840698242,
0.05048802122473717,
0.008240158669650555,
0.01507770735770464,
0.03308447077870369,
0.019452085718512535,
-0.03237752616405487,
-0.02905171923339367,
0.02803007699549198,
-0.004948488902300596,
0.01682588830590248,
0.018974296748638153,
-0.0030814344063401222,
-0.055806729942560196,
0.03008299507200718,
-0.058138132095336914,
-0.01494241040199995,
0.04204348474740982,
-0.0117113646119833,
0.05810806527733803,
-0.01233687810599804,
0.11644507199525833,
-0.020903022959828377,
-0.12212173640727997,
0.012782390229403973,
-0.020674891769886017,
0.008377422578632832,
-0.07498960196971893,
-0.04999781399965286,
0.01215052418410778,
0.0011146296747028828,
-0.00949124712496996,
0.0017993946094065905,
-0.054587140679359436,
-0.027244504541158676,
-0.05118459090590477,
-0.0010974243050441146,
0.01769365556538105,
0.015737736597657204,
-0.07916854321956635,
0.0034472583793103695,
0.019045038148760796,
0.012778589501976967,
-0.03715435788035393,
0.006455915980041027,
0.004757932387292385,
-0.003897538874298334,
0.04816612973809242,
0.05161609873175621,
-0.027077343314886093,
-0.059647805988788605,
0.006317486986517906,
-0.0030138599686324596,
-0.04766885191202164,
0.04218732938170433,
-0.06767427921295166,
0.034166961908340454,
0.04333440586924553,
-0.03732752799987793,
0.020691122859716415,
-0.03629138693213463,
-0.004501141142100096,
-0.005938530433923006,
0.00656341714784503,
0.038553327322006226,
-0.026668664067983627,
0.10275540500879288,
-0.08024166524410248,
-0.08960967510938644,
-0.011263513937592506,
0.05375160649418831,
-0.01326680090278387,
0.004460926167666912,
0.053850285708904266,
0.014519468881189823,
0.0037860104348510504,
-0.05880492553114891,
0.04686793312430382,
0.07956300675868988,
-0.028042711317539215,
-0.05817997083067894,
-0.022128179669380188,
-0.06921438872814178,
-0.023242861032485962,
-0.009433622471988201,
-0.037687331438064575,
-0.01592782512307167,
0.002554425736889243,
-0.02803337201476097,
0.03456589952111244,
-0.02920716628432274,
-0.2728951871395111,
-0.018399490043520927,
-0.0156578216701746,
0.011111638508737087,
0.04031851142644882,
-0.018909810110926628,
0.004073860589414835,
0.015037817880511284,
0.017763368785381317,
0.01820129156112671,
0.040226951241493225,
0.040249764919281006,
0.002708418294787407,
0.001017242670059204,
0.004973738919943571,
0.05539528280496597,
0.007643918506801128,
0.013391821645200253,
0.005399690009653568,
-0.007786717265844345,
0.04341142997145653,
0.01814691163599491,
0.14035598933696747,
0.053194474428892136,
-0.03757138550281525,
0.013186872005462646,
-0.0027717119082808495,
0.021482620388269424,
-0.027549071237444878,
0.0009557484881952405,
-0.017615709453821182,
-0.02077563852071762,
0.09955298155546188,
-0.003795480355620384,
0.01860070414841175,
0.020070109516382217,
-0.07176345586776733,
-0.038568221032619476,
-0.01753978617489338,
-0.0011123266303911805,
-0.00889542791992426,
-0.019689083099365234,
-0.03936004638671875,
-0.04668630659580231,
0.07063406705856323,
0.00691297696903348,
-0.01249085832387209,
-0.030470525845885277,
-0.021519936621189117,
0.014089587144553661,
-0.02519744634628296,
-0.057739678770303726,
-0.00893035251647234,
0.01486556138843298,
0.018005916848778725,
-0.0168384350836277,
-0.058907799422740936,
-0.023099906742572784,
0.045435450971126556,
0.0008790266001597047,
-0.025330593809485435,
0.057976823300123215,
0.006962515413761139,
0.06719193607568741,
0.01753746159374714
] |
PMID:27114 | [Working conditions and job-requirement of older industrial blue collar workers and the consequences in employment problems (author's transl)]. | In the following passage the reduced vocational capacity of older workers will be discussed as a consequence of the intended high-capacity of workers as well as the non--human working--conditions in industry. The reduced personal sanity and working capacity of older workers point out the high rate of handicaps and the incapacity of vocational engagement. Problems of employment--and this could be pointed out by the high rates of unemployment amongst older workers--does not signify prejudice but indicates the low profitableness of older workers in the capitalistic production system. Finally discussed will be the contribution of a monetary--conpensatoric against a more prophylactic social policy for a better solution of the vocational problems of older blue-collar workers. | [Working conditions and job-requirement of older industrial blue collar workers and the consequences in employment problems (author's transl)]. In the following passage the reduced vocational capacity of older workers will be discussed as a consequence of the intended high-capacity of workers as well as the non--human working--conditions in industry. The reduced personal sanity and working capacity of older workers point out the high rate of handicaps and the incapacity of vocational engagement. Problems of employment--and this could be pointed out by the high rates of unemployment amongst older workers--does not signify prejudice but indicates the low profitableness of older workers in the capitalistic production system. Finally discussed will be the contribution of a monetary--conpensatoric against a more prophylactic social policy for a better solution of the vocational problems of older blue-collar workers. | [
-0.03572553023695946,
0.021689893677830696,
-0.0539364330470562,
0.003625185927376151,
-0.0026453936006873846,
0.005719590932130814,
0.031152667477726936,
-0.0035914562176913023,
-0.05118725076317787,
-0.05571538582444191,
0.028619488701224327,
0.012511917389929295,
0.01178615540266037,
0.01952710747718811,
-0.01713849976658821,
0.043244268745183945,
-0.015778515487909317,
0.01754021644592285,
-0.045580122619867325,
0.04954314976930618,
0.002360263140872121,
-0.014568092301487923,
-0.048324551433324814,
0.0030861718114465475,
0.02866838313639164,
-0.0274699404835701,
-0.004428120329976082,
0.001946100383065641,
-0.029174350202083588,
-0.09891343116760254,
-0.015350705944001675,
0.018333835527300835,
0.04179512336850166,
0.03868180140852928,
0.07890629768371582,
0.04249931871891022,
0.02678844891488552,
0.004798285197466612,
-0.042394377291202545,
-0.016264796257019043,
0.02912941388785839,
0.02963157184422016,
-0.01027893926948309,
-0.03739234060049057,
-0.03046565130352974,
0.003923807758837938,
-0.009025219827890396,
0.006736738607287407,
-0.05382668972015381,
0.021509654819965363,
-0.07763627171516418,
-0.013723170384764671,
0.001373373088426888,
0.0488913394510746,
0.04714103788137436,
-0.007142196409404278,
0.028145577758550644,
0.05172576382756233,
-0.04450291022658348,
0.022739531472325325,
0.030525175854563713,
-0.061472974717617035,
-0.15287069976329803,
0.005837007891386747,
0.0666368156671524,
0.02956363372504711,
-0.08241168409585953,
-0.03132021427154541,
-0.02379058673977852,
-0.01793081872165203,
-0.0038246733602136374,
0.01733708381652832,
-0.006867825519293547,
0.029026523232460022,
0.04981839656829834,
0.0077707297168672085,
-0.04595883935689926,
-0.020077871158719063,
0.02700185216963291,
-0.006385120563209057,
0.09774156659841537,
0.05210322141647339,
0.0015547219663858414,
0.006991655565798283,
-0.02550169825553894,
-0.04256916046142578,
-0.038126397877931595,
-0.020952757447957993,
0.05802231654524803,
0.003968710545450449,
-0.022102752700448036,
0.05947922170162201,
0.025522245094180107,
0.00045684774522669613,
0.006439749151468277,
0.021913835778832436,
0.04370934143662453,
0.04082562029361725,
-0.08771439641714096,
0.45555412769317627,
0.013036413118243217,
0.014029613696038723,
0.03919648751616478,
-0.047587256878614426,
0.005146190524101257,
0.016958381980657578,
-0.04887782037258148,
0.01771591417491436,
0.028334539383649826,
0.0007013522554188967,
-0.028476126492023468,
0.009657956659793854,
0.031955745071172714,
0.05805186182260513,
-0.0011239381274208426,
-0.021285125985741615,
0.0038808456156402826,
0.03978230059146881,
0.0030517815612256527,
0.03325539082288742,
0.0477614551782608,
0.028924046084284782,
0.014147430658340454,
0.022384123876690865,
-0.0018443301087245345,
-0.02569032460451126,
-0.00940735638141632,
0.06675641983747482,
-0.01344488188624382,
-0.0448206327855587,
-0.06789188086986542,
-0.060060981661081314,
0.01587284356355667,
-0.00245562382042408,
-0.029205501079559326,
-0.003800762351602316,
-0.020024094730615616,
0.058491919189691544,
0.026211563497781754,
0.03717314824461937,
0.03500460833311081,
-0.05221438780426979,
-0.020224597305059433,
-0.06505066901445389,
-0.00892836507409811,
0.06793225556612015,
0.06814907491207123,
-0.029991203919053078,
-0.010281767696142197,
0.008328741416335106,
-0.03222215175628662,
0.020703349262475967,
0.02452063001692295,
0.0037994333542883396,
-0.016601216048002243,
-0.009753580205142498,
-0.02368973009288311,
0.04497288912534714,
-0.04444517567753792,
-0.026270508766174316,
0.03093365766108036,
-0.04921421408653259,
-0.05927017331123352,
0.05929281562566757,
0.00919069442898035,
0.005761680193245411,
-0.0664285197854042,
-0.00825646985322237,
0.02808186411857605,
-0.010003216564655304,
0.02686227858066559,
0.00205605779774487,
-0.014826062135398388,
0.02170274406671524,
0.04693400859832764,
0.043077077716588974,
0.023592164739966393,
0.061775125563144684,
-0.07289087027311325,
0.08316957950592041,
0.011976189911365509,
-0.02837228588759899,
-0.07124397158622742,
-0.01099342666566372,
-0.04672040045261383,
0.009377429261803627,
-0.0055774543434381485,
0.010587443597614765,
0.0212912205606699,
0.0020934336353093386,
-0.002377355471253395,
0.05456637963652611,
-0.07423628866672516,
-0.012690613977611065,
0.007927214726805687,
-0.004787154030054808,
-0.030598685145378113,
0.0033955471590161324,
-0.015134786255657673,
-0.012551302090287209,
-0.045703623443841934,
-0.011492518708109856,
-0.02558153308928013,
0.04929454252123833,
-0.032045502215623856,
0.03304268792271614,
-0.018735378980636597,
-0.04582299664616585,
0.0554712750017643,
-0.03337571397423744,
0.022696666419506073,
-0.10109684616327286,
0.09216108173131943,
0.06402986496686935,
0.03183109685778618,
0.04925070330500603,
0.04350324720144272,
0.02496867999434471,
0.044899411499500275,
-0.006434231530874968,
0.07242410629987717,
0.006204746197909117,
-0.04695495590567589,
-0.3308241367340088,
-0.0323035903275013,
-0.03382882848381996,
-0.026339344680309296,
0.006929270923137665,
-0.0032395997550338507,
0.0016368976794183254,
0.014039074070751667,
-0.033114608377218246,
0.01372536551207304,
0.04216353967785835,
0.009242476895451546,
-0.047247327864170074,
-0.021765949204564095,
0.053192850202322006,
-0.032678015530109406,
-0.03637722134590149,
-0.021352937445044518,
-0.036084167659282684,
-0.01910588890314102,
0.007254280149936676,
-0.0026628209743648767,
-0.010061093606054783,
-0.005051950458437204,
0.055052921175956726,
-0.06756791472434998,
0.1299794614315033,
-0.11094580590724945,
-0.05579065531492233,
-0.04761829972267151,
-0.029055921360850334,
0.029159294441342354,
-0.051042407751083374,
-0.05276495963335037,
0.023426640778779984,
0.0010160293895751238,
-0.014078168198466301,
0.010500608943402767,
-0.01692216284573078,
0.03565848246216774,
-0.024162812158465385,
-0.00968491192907095,
-0.07682076096534729,
0.04997948929667473,
-0.10704679042100906,
-0.001508055254817009,
0.01789862848818302,
0.012382512912154198,
-0.05208701640367508,
0.0004923747619614005,
0.006876959465444088,
0.07237190008163452,
-0.02340262196958065,
0.047112882137298584,
0.009137357585132122,
-0.07576650381088257,
-0.0909338891506195,
0.032401420176029205,
-0.030167773365974426,
-0.01567857153713703,
-0.004326278809458017,
-0.004905622452497482,
0.07899712771177292,
-0.009798646904528141,
0.05390552803874016,
-0.0055695585906505585,
0.006549299228936434,
0.028557319194078445,
-0.009445440955460072,
-0.021828817203640938,
0.047824326902627945,
0.09836186468601227,
0.03656524792313576,
-0.06303228437900543,
0.02458301931619644,
0.0053514777682721615,
-0.012868057005107403,
0.026318881660699844,
0.06246932968497276,
0.024405546486377716,
0.05756095051765442,
-0.08264857530593872,
0.00417899526655674,
0.09426689893007278,
0.05239886790513992,
-0.02769196219742298,
-0.011162061244249344,
0.00664245430380106,
0.05620955675840378,
0.035584598779678345,
-0.03627755492925644,
0.03382164612412453,
-0.07144881039857864,
-0.009721370413899422,
0.04511624202132225,
0.01625114306807518,
-0.26863646507263184,
0.0023993595968931913,
-0.008442078717052937,
-0.026808716356754303,
0.0012866150354966521,
-0.0034752944484353065,
-0.006071566604077816,
-0.034635014832019806,
-0.03675620257854462,
0.032687485218048096,
0.04819092899560928,
0.04694190248847008,
0.027604226022958755,
0.009972501546144485,
-0.004286576062440872,
-0.014943714253604412,
-0.004275192506611347,
-0.02820996195077896,
0.026036148890852928,
-0.03502083197236061,
-0.002139193704351783,
0.057568226009607315,
0.08877038955688477,
-0.011242435313761234,
-0.008273578248918056,
0.001010079518891871,
0.02790917456150055,
-0.014977563172578812,
0.004476754926145077,
-0.07643495500087738,
0.007144199218600988,
-0.04153722897171974,
0.10002022981643677,
-0.020861050114035606,
-0.011555581353604794,
-0.042657870799303055,
-0.0712108239531517,
0.013167244382202625,
0.004475415218621492,
0.01611369475722313,
-0.03592653572559357,
0.00048813235480338335,
-0.026735957711935043,
-0.0008632163517177105,
0.09475991129875183,
0.008079961873590946,
-0.030975038185715675,
-0.012299402616918087,
0.05469425395131111,
0.01977064087986946,
-0.016590291634202003,
0.0010654443176463246,
0.011135418899357319,
0.06905297189950943,
-0.013819608837366104,
0.0069155204109847546,
0.023723753169178963,
-0.02887624315917492,
0.02084162086248398,
-0.04629728943109512,
-0.0438888780772686,
0.028765840455889702,
-0.05897342413663864,
0.046877067536115646,
-0.0022430249955505133
] |
PMID:27115 | [The effects of the social security system on the life situation of old people (author's transl)]. | The situation of old people is determined by the system of Social Security in a various way. A point of most importance are effects of pensions etc. on the distribution of income. It is shown, that distribution of old-age pensions follows the same scheme, which is dominating in the distribution of primary income. This is due to the basic principles of the Social Security System, which intensify inequality. | [The effects of the social security system on the life situation of old people (author's transl)]. The situation of old people is determined by the system of Social Security in a various way. A point of most importance are effects of pensions etc. on the distribution of income. It is shown, that distribution of old-age pensions follows the same scheme, which is dominating in the distribution of primary income. This is due to the basic principles of the Social Security System, which intensify inequality. | [
-0.012954751029610634,
0.01090281829237938,
-0.0326831117272377,
-0.052532296627759933,
0.05251750349998474,
0.03163471072912216,
0.06800427287817001,
-0.03508356213569641,
-0.036186594516038895,
0.04555824398994446,
0.016608022153377533,
0.07831449806690216,
0.010216454043984413,
0.018151819705963135,
0.011393837630748749,
-0.01888621784746647,
-0.059698935598134995,
0.03716801851987839,
-0.05621373653411865,
0.08003048598766327,
0.051757585257291794,
-0.04006166756153107,
-0.06720968335866928,
-0.037837885320186615,
0.07196290791034698,
0.036204393953084946,
-0.028547698631882668,
0.00020686349307652563,
-0.06955426186323166,
-0.1249348446726799,
-0.0036993701942265034,
-0.011472474783658981,
0.016404127702116966,
-0.00831522885710001,
-0.008628776296973228,
0.0005015747155994177,
0.03127019479870796,
0.026032142341136932,
-0.01206072885543108,
-0.01582898199558258,
-0.033358801156282425,
0.04494988173246384,
-0.0034038035664707422,
-0.061177756637334824,
-0.0038971290923655033,
0.02919309213757515,
-0.04467892646789551,
-0.010942388325929642,
-0.044783566147089005,
-0.03441387414932251,
-0.039547041058540344,
0.02145383134484291,
0.02753318101167679,
0.09705788642168045,
0.011001763865351677,
-0.04043806716799736,
0.03216706961393356,
0.05528177693486214,
-0.011334179900586605,
-0.0028682195115834475,
0.01427590474486351,
-0.03243986517190933,
-0.1508605182170868,
0.005359928123652935,
0.05507289990782738,
0.049404390156269073,
0.012483450584113598,
0.01797173172235489,
0.027847489342093468,
-0.03862830996513367,
-0.06534339487552643,
0.058981020003557205,
0.05007008835673332,
0.024759767577052116,
0.018152497708797455,
-0.057655829936265945,
-0.0353243388235569,
-0.010995570570230484,
-0.029974380508065224,
0.014231450855731964,
0.05428525060415268,
0.047566790133714676,
0.023333124816417694,
0.028890931978821754,
-0.00016053994477260858,
0.004737874027341604,
0.012523955665528774,
-0.0766117200255394,
0.017108239233493805,
-0.04294876009225845,
0.012467107735574245,
0.05641654133796692,
0.014531646855175495,
0.03718959912657738,
0.06313900649547577,
-0.03479599952697754,
-0.002498459070920944,
-0.0031915842555463314,
0.008022553287446499,
0.3757266700267792,
0.04240645468235016,
0.0880422294139862,
0.0436171256005764,
-0.0009388698381371796,
0.03577268123626709,
0.006751718930900097,
-0.047039762139320374,
-0.014848465099930763,
0.006647165399044752,
0.024311741814017296,
-0.0033415830694139004,
-0.021438349038362503,
-0.042056530714035034,
0.018353603780269623,
0.0060332054272294044,
-0.06663776934146881,
0.04847792536020279,
0.052027516067028046,
0.03633779659867287,
-0.05210140347480774,
-0.008375770412385464,
0.05553147569298744,
-0.00742748100310564,
0.013756842352449894,
0.03446359559893608,
0.011918112635612488,
0.03237846493721008,
0.09330698847770691,
0.018146473914384842,
-0.021882928907871246,
0.020562103018164635,
-0.05538857728242874,
-0.02410051040351391,
-0.0005397499189712107,
-0.00982749741524458,
0.039756059646606445,
-0.06373699754476547,
0.04180873557925224,
0.04455316439270973,
-0.010582348331809044,
0.05576613172888756,
-0.04098295792937279,
-0.057112764567136765,
-0.0398448184132576,
-0.03586025908589363,
0.12110532075166702,
0.08222517371177673,
0.008206062950193882,
0.019495610147714615,
0.0139703918248415,
-0.009662666358053684,
-0.008277489803731441,
0.0312168151140213,
-0.00220022932626307,
-0.05779007822275162,
0.018203506246209145,
-0.04208633303642273,
-0.016545874997973442,
-0.019499242305755615,
-0.0673028975725174,
-0.01280639972537756,
-0.025194508954882622,
-0.022874612361192703,
0.08174631744623184,
0.031268034130334854,
-0.00233525806106627,
-0.04166024178266525,
0.027611086145043373,
0.06271737068891525,
0.021127887070178986,
0.06070776656270027,
-0.02749069593846798,
-0.013445544987916946,
0.03655925393104553,
0.0731004998087883,
0.05616804584860802,
0.06599847972393036,
-0.01498564425855875,
-0.0612996369600296,
-0.002345788525417447,
0.013033192604780197,
0.005083164200186729,
-0.030662834644317627,
0.0025656032375991344,
-0.051314983516931534,
-0.011400805786252022,
-0.07326264679431915,
0.030856311321258545,
0.014587501995265484,
-0.028188176453113556,
0.022538289427757263,
0.05414099246263504,
-0.08841549605131149,
-0.05544930696487427,
0.010126383975148201,
-0.024325452744960785,
-0.05558426305651665,
0.009299998171627522,
-0.029816679656505585,
0.005400649271905422,
-0.010816123336553574,
-0.01464629452675581,
0.01381621789187193,
-0.028278673067688942,
-0.0216271560639143,
0.04752900078892708,
-0.016755595803260803,
-0.09170044213533401,
0.0188435185700655,
0.017066605389118195,
-0.01740310527384281,
-0.02882484346628189,
0.08794119954109192,
0.022144323214888573,
0.04924033209681511,
0.08191033452749252,
0.027015559375286102,
0.005526619032025337,
0.054884858429431915,
0.01323686446994543,
0.07473138719797134,
0.010076094418764114,
-0.006229131016880274,
-0.3119859993457794,
-0.0438670814037323,
-0.0220956951379776,
-0.08001037687063217,
-0.011461375281214714,
0.012624111026525497,
0.0022543740924447775,
0.021291323006153107,
-0.02021496742963791,
0.011089159175753593,
0.11956963688135147,
0.0032383298967033625,
0.05521632730960846,
0.015400571748614311,
0.0466441884636879,
-0.022831328213214874,
-0.05667169392108917,
-0.02534804493188858,
-0.029184972867369652,
0.012168226763606071,
-0.021704938262701035,
-0.03034784272313118,
0.030190227553248405,
0.0009597540483810008,
0.10203605145215988,
-0.027667202055454254,
0.06756336241960526,
-0.09056951850652695,
-0.07415459305047989,
-0.0038823906797915697,
-0.001492985524237156,
0.032342568039894104,
-0.0030944482423365116,
-0.04923304542899132,
0.06797301024198532,
0.01460236869752407,
0.011899568140506744,
-0.021532289683818817,
-0.051786575466394424,
-0.023601699620485306,
-0.03966398164629936,
-0.03200786933302879,
-0.014243638142943382,
0.06448697298765182,
-0.09893950074911118,
-0.010431528091430664,
0.012723393738269806,
0.004939142614603043,
0.0002661167527548969,
0.025108367204666138,
0.015070303343236446,
-0.002541515277698636,
0.00614063860848546,
0.04817476496100426,
0.09075520187616348,
-0.04368441924452782,
-0.05346352234482765,
-0.0030040666460990906,
-0.03906606137752533,
0.02571207657456398,
-0.04012959077954292,
0.005299031268805265,
0.08701475709676743,
-0.04192746803164482,
0.029565250501036644,
-0.057442836463451385,
-0.03886944428086281,
-0.014000341296195984,
-0.0584401935338974,
-0.0058397152461111546,
0.007096577901393175,
0.10088823735713959,
-0.05669074505567551,
-0.02059856243431568,
0.006957242265343666,
0.06621582061052322,
0.05612950772047043,
0.002324197441339493,
0.03238702937960625,
-0.012134579010307789,
0.021165408194065094,
-0.07267103344202042,
0.011244314722716808,
0.0732956975698471,
0.018670769408345222,
-0.07605137676000595,
-0.02485397458076477,
0.002026887843385339,
-0.018499676138162613,
0.010289594531059265,
-0.08006705343723297,
0.0057806153781712055,
-0.06565622985363007,
0.0021725029218941927,
-0.009515379555523396,
-0.00568554038181901,
-0.2525242269039154,
0.07836037129163742,
-0.045675963163375854,
-0.006298100110143423,
0.0208381786942482,
-0.004232593346387148,
-0.059443626552820206,
0.016444889828562737,
-0.0049482062458992004,
-0.0019583257380872965,
0.059208087623119354,
0.09392251074314117,
0.06132145971059799,
-0.041151050478219986,
-0.022412549704313278,
-0.018270332366228104,
-0.01815386489033699,
-0.049358952790498734,
-0.006943064276129007,
-0.036177266389131546,
-0.001453498611226678,
0.05386495217680931,
0.12586845457553864,
0.0049221813678741455,
0.013075275346636772,
-0.04526257514953613,
-0.02109348773956299,
0.004074934404343367,
-0.039413075894117355,
-0.03549676761031151,
0.0024677044712007046,
0.00533553771674633,
-0.008754358626902103,
-0.01767665520310402,
-0.03629791736602783,
0.04868185892701149,
-0.054096441715955734,
-0.0005386730190366507,
-0.0020105959847569466,
0.03978560492396355,
-0.04342520982027054,
-0.014387776143848896,
0.013851689174771309,
-0.009526328183710575,
0.10560780763626099,
-0.03800731152296066,
0.00044487821287475526,
-0.043715547770261765,
0.059134840965270996,
0.02081041969358921,
-0.06038451939821243,
-0.049377650022506714,
-0.005278868600726128,
0.04032254219055176,
0.015341983176767826,
-0.03317650035023689,
-0.0550159253180027,
0.004148892126977444,
0.07786965370178223,
-0.03415219858288765,
-0.020431600511074066,
-0.008631756529211998,
-0.020949920639395714,
-0.015700822696089745,
-0.0033554150722920895
] |
PMID:27116 | [Maintenance payments to the elderly (author's transl)]. | The question of the extent older people sustain their livelihood through financial contribution from their children is important sociopolitically inasmuch as claims for payments under the Federal Social Welfare Scheme (Bundessozialhilfegesetz) can only be made where no such private financial support can be given by those next of kin responsible in law. It has been established that in approximately every 14th household in the Federal Republic such maintenance payments are made to at least one member of the older generation (either parents and/or parents-in-law). Seen together with the findings of other inquiries it appears justifiable to assume that these payments are more often remunerations recompensed either materially or otherwise by the older generation rather than a genuine alternative to (necessary) public welfare support. It must therefore be assumed that onesided private financial support carries the odium of "private relief" for the recipients, in which case it would run contrary to the intentions of social legislation. | [Maintenance payments to the elderly (author's transl)]. The question of the extent older people sustain their livelihood through financial contribution from their children is important sociopolitically inasmuch as claims for payments under the Federal Social Welfare Scheme (Bundessozialhilfegesetz) can only be made where no such private financial support can be given by those next of kin responsible in law. It has been established that in approximately every 14th household in the Federal Republic such maintenance payments are made to at least one member of the older generation (either parents and/or parents-in-law). Seen together with the findings of other inquiries it appears justifiable to assume that these payments are more often remunerations recompensed either materially or otherwise by the older generation rather than a genuine alternative to (necessary) public welfare support. It must therefore be assumed that onesided private financial support carries the odium of "private relief" for the recipients, in which case it would run contrary to the intentions of social legislation. | [
-0.040990136563777924,
0.013622653670608997,
-0.040039483457803726,
-0.0486893393099308,
0.07367405295372009,
0.03103688359260559,
0.013959919102489948,
-0.03034723363816738,
-0.06064579263329506,
-0.030813617631793022,
-0.0025503148790448904,
-0.012316577136516571,
-0.028710180893540382,
-0.008038093335926533,
-0.02229088358581066,
0.0002760492207016796,
-0.006083828397095203,
0.05379386991262436,
0.021610256284475327,
0.04253065213561058,
0.004866630304604769,
-0.07304355502128601,
0.010045486502349377,
0.015457133762538433,
0.05450531840324402,
0.022829262539744377,
0.024782508611679077,
-0.027652015909552574,
-0.010133108124136925,
-0.12707224488258362,
0.017191436141729355,
-0.04803195595741272,
-0.023160848766565323,
0.015216981992125511,
0.049674466252326965,
0.0006688198773190379,
0.049230996519327164,
-0.04014471918344498,
-0.010919851250946522,
0.020732678472995758,
-0.03887266293168068,
0.0653049647808075,
-0.03492762893438339,
-0.02410968206822872,
-0.010051138699054718,
0.049633655697107315,
0.026602914556860924,
-0.015731289982795715,
-0.05348922684788704,
0.019466092810034752,
-0.02689732424914837,
0.04523627832531929,
0.02461187168955803,
0.05139469727873802,
-0.0256495401263237,
0.018168428912758827,
0.02517932653427124,
0.02247662842273712,
0.00702642509713769,
0.008826361037790775,
0.02854372188448906,
0.0021841833367943764,
-0.19284546375274658,
0.0061572240665555,
0.0021833213977515697,
0.07127591222524643,
0.040990982204675674,
-0.016071774065494537,
-0.04069698229432106,
-0.003420829074457288,
-0.01167111936956644,
-0.008180768229067326,
-0.042501743882894516,
0.002344997599720955,
0.029244832694530487,
-0.01775936409831047,
0.03533179312944412,
-0.005044583231210709,
-0.05865810438990593,
0.002695610746741295,
0.000032045358238974586,
0.04458167031407356,
0.006181247066706419,
0.049717146903276443,
-0.05176188051700592,
-0.03514285013079643,
0.00008837066707201302,
-0.05111441761255264,
0.04591643437743187,
0.018993239849805832,
-0.0580877847969532,
0.07175996899604797,
0.05411157384514809,
-0.006995284464210272,
0.022452620789408684,
-0.03729451447725296,
0.00703327264636755,
0.07852263748645782,
-0.058464132249355316,
0.48473525047302246,
0.027403613552451134,
0.057110585272312164,
0.03646176680922508,
-0.00967311579734087,
0.010166773572564125,
0.022123636677861214,
-0.011596174910664558,
0.03118763491511345,
0.04305851459503174,
-0.014548176899552345,
-0.08302900940179825,
-0.03588562458753586,
0.025987522676587105,
0.009673064574599266,
0.027302643284201622,
-0.060081105679273605,
0.025566786527633667,
0.04778845235705376,
-0.0010308584896847606,
-0.014723595231771469,
0.000710661115590483,
0.034936293959617615,
-0.03820788487792015,
-0.03609646111726761,
0.006339056417346001,
-0.04172633960843086,
-0.011054915376007557,
0.05847647413611412,
0.015782291069626808,
0.035244449973106384,
0.023875102400779724,
-0.04446884244680405,
-0.02038402296602726,
-0.02582087740302086,
0.005082994699478149,
-0.004509820137172937,
-0.027820179238915443,
0.015126426704227924,
-0.003333382774144411,
0.009849325753748417,
0.056211236864328384,
-0.0945427268743515,
-0.063511922955513,
-0.03799660503864288,
-0.059384785592556,
0.06613533943891525,
0.06993529200553894,
0.03807805851101875,
0.0117922592908144,
0.024868354201316833,
-0.01825174316763878,
0.0780394896864891,
0.04075543209910393,
-0.054094068706035614,
0.026974288746714592,
0.015383248217403889,
0.01901632361114025,
-0.01581653021275997,
-0.04446830227971077,
-0.044016581028699875,
0.08024237304925919,
-0.04099944606423378,
-0.06194280833005905,
0.07633385807275772,
0.03262244910001755,
-0.01605658419430256,
-0.01804209128022194,
-0.0024374686181545258,
0.039935093373060226,
-0.02137603610754013,
0.016417698934674263,
0.007959984242916107,
0.000006097210643929429,
-0.009807956404983997,
0.084996797144413,
0.003285470185801387,
0.008515842258930206,
-0.0024068793281912804,
-0.028841540217399597,
0.06474822759628296,
-0.028192322701215744,
-0.05821182206273079,
-0.050905242562294006,
-0.0016656725201755762,
-0.013368485495448112,
0.007564940955489874,
-0.05619104206562042,
-0.012038362212479115,
0.01754262112081051,
-0.03252618759870529,
-0.015170321799814701,
0.02949913591146469,
-0.03500976040959358,
0.012106677517294884,
0.02431536465883255,
0.008192194625735283,
-0.0791543498635292,
-0.008414175361394882,
0.007687848061323166,
-0.0011042100377380848,
-0.0013776208506897092,
0.013094072230160236,
-0.0028515984304249287,
-0.016322828829288483,
-0.0102416155859828,
0.02233569696545601,
0.03999859094619751,
-0.04142334312200546,
0.07754138857126236,
-0.013053059577941895,
0.02088804356753826,
0.04829324409365654,
0.05273934081196785,
0.02268207259476185,
-0.01272672414779663,
0.07055928558111191,
0.027578577399253845,
-0.005547680426388979,
0.020598558709025383,
0.0009032116504386067,
0.03661184012889862,
-0.022367913275957108,
0.022914880886673927,
-0.29240384697914124,
-0.05581222474575043,
-0.05148890241980553,
-0.021418312564492226,
-0.004830022342503071,
-0.012268374674022198,
0.0179483350366354,
-0.03626805171370506,
-0.08313027769327164,
0.0024263409432023764,
0.05776147171854973,
0.0017446177080273628,
-0.0168845783919096,
0.048193883150815964,
0.048011116683483124,
-0.017608512192964554,
-0.01984631083905697,
0.001098459935747087,
-0.04437216743826866,
-0.0010554157197475433,
-0.028735043480992317,
-0.02369043417274952,
0.05835035443305969,
-0.015369748696684837,
0.11987477540969849,
0.008034720085561275,
0.145352303981781,
-0.01001491118222475,
-0.01823246479034424,
-0.05013718828558922,
-0.002039968501776457,
0.038099389523267746,
-0.013985419645905495,
-0.09204819053411484,
0.0019062228966504335,
0.002641942584887147,
0.00443124258890748,
-0.08519437164068222,
-0.0006831061909906566,
-0.006017740350216627,
0.008364442735910416,
0.038457177579402924,
-0.03845590725541115,
0.016625795513391495,
-0.08254099637269974,
-0.011484905146062374,
-0.0036017526872456074,
-0.03808952122926712,
0.014952221885323524,
0.04049769788980484,
-0.020474566146731377,
-0.040841635316610336,
-0.026401568204164505,
0.06505431979894638,
0.021630963310599327,
-0.034323785454034805,
-0.014562198892235756,
0.009706969372928143,
-0.0889289379119873,
0.004575655795633793,
-0.036473412066698074,
0.018559545278549194,
0.09354620426893234,
-0.06732342392206192,
0.09330721944570541,
-0.0800694152712822,
-0.05623811483383179,
0.0059734731912612915,
-0.047732654958963394,
0.004044625908136368,
0.026833241805434227,
0.0825517550110817,
-0.09184954315423965,
-0.060232024639844894,
-0.008013881742954254,
0.04868031665682793,
0.045358192175626755,
-0.006839608307927847,
0.020133858546614647,
0.0006406115717254579,
0.049790821969509125,
-0.05692356452345848,
0.006849739700555801,
0.06395099312067032,
-0.013049636967480183,
-0.02351573295891285,
-0.03534943610429764,
0.016736365854740143,
0.012812109664082527,
-0.015540783293545246,
-0.0011028936132788658,
0.07963704317808151,
-0.06009727343916893,
-0.01300598680973053,
0.012584452517330647,
0.023332878947257996,
-0.22408710420131683,
-0.008496224880218506,
-0.03210708871483803,
-0.050210610032081604,
-0.012595984153449535,
0.015228557400405407,
-0.018559666350483894,
0.0021078852005302906,
-0.04183458536863327,
0.014059331268072128,
0.06514924019575119,
0.10203520208597183,
0.023470906540751457,
-0.0016275199595838785,
0.04666493833065033,
-0.008599000982940197,
-0.013347088359296322,
-0.03957337141036987,
-0.025403983891010284,
-0.034434668719768524,
0.05942239612340927,
0.000011663873010547832,
0.0947541818022728,
0.001414154190570116,
-0.018107876181602478,
-0.0032564853318035603,
-0.042882148176431656,
0.006615108344703913,
0.05834188312292099,
-0.004202547483146191,
-0.03523990884423256,
0.01415493804961443,
0.041713353246450424,
-0.009360931813716888,
0.04899681732058525,
-0.02659960463643074,
-0.03300275653600693,
0.019035516306757927,
-0.006589988246560097,
0.016658451408147812,
-0.03337249904870987,
-0.001668678130954504,
-0.010081242769956589,
-0.02927134744822979,
0.06565333902835846,
-0.016213465481996536,
-0.010895822197198868,
-0.028971347957849503,
0.014899324625730515,
0.01706693321466446,
-0.028674587607383728,
-0.008614834398031235,
-0.001526141189970076,
0.021640794351696968,
0.029884198680520058,
-0.0456407256424427,
-0.004064786247909069,
0.008915101177990437,
0.06776322424411774,
-0.02308577485382557,
-0.03327897563576698,
0.043114375323057175,
-0.028791502118110657,
0.074917733669281,
-0.03017830289900303
] |
PMID:27117 | [Measuring nursing needs of older people related to actual nursing time in institutions for the aged (author's transl)]. | A great many of the elderly people are in need of care. In offering services accordingly it is necessary to acquire relevant data. The following article gives results of a study which determined 1) the real amount of given nursing care and 2) the measured amount of nursing needs of these patients. The research was done at 10 nursing stations with 210 persons. The results make clear 1) that need of care must be seen in a multidemensional perspective and 2) that much of the psychological and social needs of the patients are not yet satisfied. | [Measuring nursing needs of older people related to actual nursing time in institutions for the aged (author's transl)]. A great many of the elderly people are in need of care. In offering services accordingly it is necessary to acquire relevant data. The following article gives results of a study which determined 1) the real amount of given nursing care and 2) the measured amount of nursing needs of these patients. The research was done at 10 nursing stations with 210 persons. The results make clear 1) that need of care must be seen in a multidemensional perspective and 2) that much of the psychological and social needs of the patients are not yet satisfied. | [
-0.0040091872215271,
-0.031996991485357285,
-0.015114154666662216,
-0.05680608004331589,
0.03959667682647705,
0.0025408328510820866,
0.014535642229020596,
0.002498307963833213,
-0.01534495409578085,
-0.010434705764055252,
0.0212967861443758,
-0.03083721548318863,
-0.034838154911994934,
0.05664656311273575,
0.022935902699828148,
0.029880957677960396,
-0.016900651156902313,
-0.012488949112594128,
-0.00914683286100626,
0.1083129346370697,
-0.00137627637013793,
0.00885673239827156,
0.003954537678509951,
0.024538515135645866,
0.018213769420981407,
-0.010388068854808807,
0.022953102365136147,
-0.07729966193437576,
-0.03062978759407997,
-0.08184853196144104,
-0.04147816076874733,
0.04470877721905708,
0.11088941246271133,
-0.00408575776964426,
0.002763795666396618,
0.005012104753404856,
0.0381661131978035,
0.009107008576393127,
0.02312895841896534,
-0.005689426325261593,
0.003878298681229353,
0.013174443505704403,
0.027993516996502876,
-0.040754400193691254,
-0.024770155549049377,
-0.014400588348507881,
-0.06422385573387146,
-0.02302376739680767,
0.025673476979136467,
0.04578135162591934,
0.002790268510580063,
0.03197722136974335,
0.009423197247087955,
0.08336188644170761,
0.06558911502361298,
-0.016807829961180687,
0.04785972833633423,
0.001010756939649582,
0.013913871720433235,
-0.00045604933984577656,
0.06646274775266647,
-0.06072369962930679,
-0.14066649973392487,
0.005697943270206451,
-0.047379590570926666,
0.0010498996125534177,
-0.019189195707440376,
-0.03226306661963463,
0.030635667964816093,
0.03753452003002167,
-0.07131298631429672,
-0.01771101914346218,
-0.012775871902704239,
0.05183839052915573,
0.02013585716485977,
-0.05551888421177864,
-0.008347278460860252,
-0.031454868614673615,
0.015850884839892387,
-0.010595675557851791,
0.06193089485168457,
0.059420015662908554,
0.00862826406955719,
-0.0060296738520264626,
-0.05386122688651085,
-0.021422352641820908,
0.008088832721114159,
-0.03289586305618286,
-0.002083108527585864,
-0.01398715004324913,
-0.005797588266432285,
0.0000921332830330357,
-0.06861273944377899,
0.07229182869195938,
0.04394267126917839,
-0.06805459409952164,
-0.00848634634166956,
0.07209566235542297,
-0.043763335794210434,
0.41048911213874817,
-0.0029400670900940895,
0.009735971689224243,
0.06346017122268677,
-0.0020075226202607155,
-0.0016132979653775692,
-0.03535197675228119,
0.023330749943852425,
-0.03542601317167282,
-0.0024330990854650736,
0.057463448494672775,
-0.07649585604667664,
-0.01754739321768284,
0.021927520632743835,
-0.03209884092211723,
0.013938563875854015,
0.03884665668010712,
-0.006281929090619087,
-0.0015290320152416825,
0.03666074946522713,
-0.017954396083950996,
0.016722148284316063,
0.004050191026180983,
0.025980282574892044,
0.006570322439074516,
0.0450064092874527,
-0.05294947326183319,
0.015652794390916824,
0.0512206107378006,
-0.008209696970880032,
-0.013886593282222748,
-0.004107894841581583,
-0.04028688371181488,
-0.04044289141893387,
-0.04873042553663254,
-0.036587927490472794,
-0.009930172935128212,
0.038049787282943726,
-0.015149868093430996,
0.027260031551122665,
-0.013158749788999557,
0.030850160866975784,
-0.11450528353452682,
0.0028319789562374353,
-0.06906998157501221,
-0.08629117161035538,
0.13980449736118317,
0.0318758487701416,
0.06525310128927231,
-0.045163124799728394,
-0.022162001579999924,
0.009790491312742233,
0.10639457404613495,
-0.01587442308664322,
-0.055297382175922394,
0.026619872078299522,
0.015583401545882225,
-0.0028679384849965572,
-0.01477535255253315,
-0.03897472470998764,
-0.04953223466873169,
0.04837734252214432,
-0.06931697577238083,
-0.06458991765975952,
0.03591378033161163,
-0.0221860334277153,
-0.0309310182929039,
-0.005600344855338335,
0.02682781033217907,
-0.04614216834306717,
0.026051640510559082,
0.0363304503262043,
0.0022511128336191177,
-0.05032457411289215,
0.011652585119009018,
0.07574541121721268,
0.02277597039937973,
0.03970623016357422,
-0.00019677207455970347,
-0.007444389164447784,
-0.0003494291740935296,
-0.016018109396100044,
0.05045957863330841,
0.0037374249659478664,
0.028718601912260056,
-0.010368741117417812,
-0.016533439978957176,
0.03422708436846733,
0.01540591660887003,
0.018504677340388298,
-0.04284600913524628,
-0.018487799912691116,
0.0059894295409321785,
-0.010181008838117123,
0.006266965065151453,
0.018156927078962326,
-0.003872118191793561,
-0.017794568091630936,
0.03058347851037979,
-0.032519105821847916,
0.02529970556497574,
0.0030909283086657524,
-0.013216199353337288,
0.020947618409991264,
0.03792408108711243,
-0.004088056273758411,
0.0544181764125824,
0.031014317646622658,
0.023289553821086884,
0.06081138551235199,
0.036646027117967606,
-0.023371005430817604,
0.030681436881422997,
0.06619082391262054,
0.014519330114126205,
-0.06292255967855453,
0.03907684609293938,
0.011846177279949188,
-0.051593516021966934,
0.032423846423625946,
0.07008716464042664,
0.05391435697674751,
0.061512745916843414,
-0.037906259298324585,
-0.3207942843437195,
-0.013300729915499687,
-0.0018679886125028133,
0.013830936513841152,
0.023766493424773216,
0.03418160602450371,
-0.04348068684339523,
0.013425050303339958,
-0.06722643226385117,
0.026578372344374657,
0.055685289204120636,
0.00655574444681406,
-0.06629664450883865,
0.015295120887458324,
-0.01438811793923378,
-0.0050657568499445915,
0.011730515398085117,
-0.02968651056289673,
-0.10798408836126328,
-0.07402589917182922,
0.026562051847577095,
0.03850572928786278,
0.061704859137535095,
0.05268968641757965,
0.06403767317533493,
-0.01608743518590927,
0.1350419968366623,
-0.11838771402835846,
-0.06219538301229477,
-0.049451496452093124,
-0.014592458494007587,
-0.03181516006588936,
-0.03645404055714607,
-0.07371287047863007,
0.03271514177322388,
-0.07853220403194427,
-0.08711123466491699,
0.042539142072200775,
0.00015135592548176646,
-0.009699595160782337,
-0.05686529353260994,
0.04581056907773018,
-0.03286048024892807,
0.07045313715934753,
-0.04632465913891792,
0.04192370921373367,
0.02161201275885105,
0.015192165970802307,
-0.07563690096139908,
-0.014226710423827171,
-0.04333459958434105,
0.004807190038263798,
-0.02822578139603138,
0.030093833804130554,
-0.06588216125965118,
0.041854772716760635,
-0.06586248427629471,
-0.017920657992362976,
-0.04381943866610527,
0.027122680097818375,
-0.025917187333106995,
0.07579560577869415,
0.028153235092759132,
-0.02518068253993988,
-0.003354637185111642,
-0.04283079504966736,
-0.041195012629032135,
0.036855850368738174,
-0.008058732375502586,
0.002567701041698456,
0.02621849812567234,
0.0700189620256424,
-0.08525190502405167,
-0.02578265778720379,
0.034118033945560455,
0.0111033720895648,
0.006834295112639666,
-0.007110988721251488,
0.04852088540792465,
0.02551085129380226,
0.0200028233230114,
-0.08193201571702957,
-0.05017242580652237,
0.0707327127456665,
0.032902009785175323,
0.0214094165712595,
-0.005405523348599672,
0.02267715148627758,
0.012064089067280293,
0.005861225537955761,
-0.015602046623826027,
-0.01500885933637619,
0.007121617905795574,
-0.06913845986127853,
0.02403065748512745,
-0.01265942957252264,
-0.24735599756240845,
0.04335582256317139,
0.024973466992378235,
0.01332154031842947,
-0.009823654778301716,
-0.021389644593000412,
-0.006359482649713755,
0.05655485764145851,
-0.02713196538388729,
-0.018617169931530952,
0.023842372000217438,
0.0507725365459919,
0.051290400326251984,
-0.042627137154340744,
0.014030806720256805,
0.009035191498696804,
0.03368300199508667,
-0.008588273078203201,
-0.03334127739071846,
-0.06572231650352478,
0.047426093369722366,
-0.032923031598329544,
0.14230144023895264,
-0.04473614692687988,
-0.0512232705950737,
0.012194166891276836,
0.0059348889626562595,
-0.04631175100803375,
0.051422350108623505,
-0.05069658160209656,
0.0367717444896698,
-0.00038458494236692786,
0.05515525862574577,
0.0037016505375504494,
0.039548441767692566,
0.0036496359389275312,
-0.03254682943224907,
0.018390653654932976,
-0.012497156858444214,
0.00632025022059679,
0.013622370548546314,
-0.04637998342514038,
-0.0038854533340781927,
-0.00717263575643301,
0.10083414614200592,
0.029041308909654617,
-0.0008284923969767988,
-0.0776342824101448,
0.0014590801438316703,
-0.0023163380101323128,
-0.043356653302907944,
0.004292772617191076,
0.01623285748064518,
0.010882096365094185,
0.06067176163196564,
0.009057264775037766,
0.026314975693821907,
-0.009236752055585384,
0.02506920136511326,
-0.04612109437584877,
-0.05812251940369606,
-0.01536310650408268,
0.02022060938179493,
0.03334028646349907,
-0.0031761506106704473
] |
PMID:27118 | [Environment and selfsufficiency of elder man (author's transl)]. | The problems of environment of elderly people are concentrated in social area and in the intimate living space, which can not be maintained sufficiently because of increasing age and decreasing performance. The consequences of mobility in industrial revolution scattered families and also deprived old men of their helpers. Inadequate living rooms and absence of technical comfort especially in the houses of the very aged men produce intense dependence of the own individual performance. Medical data of the spontaneous elected performance informe about the amount of the requirements of daily working capacity. They show low requirements of stress. Spontaneous working load was below of the level of medical well tolerated effort. In healthy men no signs of overloading have been registrated. Seldom removal in old age pensions before the age of eighty is indicated by illness but frequently social motivation predominates. On the contrary considerable insufficiency in social and medical providing exist after acut illness, what is to improve by special hospitals for rehabilitation of older people and by a grid of social stations. By this method it might be possible to save a great amount of money by governments that now has to be payed for exorbitant expansive assistance which is medical not indicated. | [Environment and selfsufficiency of elder man (author's transl)]. The problems of environment of elderly people are concentrated in social area and in the intimate living space, which can not be maintained sufficiently because of increasing age and decreasing performance. The consequences of mobility in industrial revolution scattered families and also deprived old men of their helpers. Inadequate living rooms and absence of technical comfort especially in the houses of the very aged men produce intense dependence of the own individual performance. Medical data of the spontaneous elected performance informe about the amount of the requirements of daily working capacity. They show low requirements of stress. Spontaneous working load was below of the level of medical well tolerated effort. In healthy men no signs of overloading have been registrated. Seldom removal in old age pensions before the age of eighty is indicated by illness but frequently social motivation predominates. On the contrary considerable insufficiency in social and medical providing exist after acut illness, what is to improve by special hospitals for rehabilitation of older people and by a grid of social stations. By this method it might be possible to save a great amount of money by governments that now has to be payed for exorbitant expansive assistance which is medical not indicated. | [
-0.024936223402619362,
0.03545438125729561,
0.032975006848573685,
0.0018071380909532309,
0.03579530119895935,
0.013102060183882713,
0.03025045432150364,
-0.02253459393978119,
-0.06156446412205696,
-0.041007451713085175,
-0.02810131385922432,
0.0037503179628401995,
-0.01877027004957199,
0.03317175805568695,
-0.01306889671832323,
0.04493483528494835,
-0.016353754326701164,
0.03804059699177742,
-0.006019668187946081,
0.07503418624401093,
-0.00712729012593627,
-0.006484449841082096,
-0.0208862517029047,
-0.000557173159904778,
0.010361332446336746,
-0.013493791222572327,
-0.029931794852018356,
-0.036713581532239914,
-0.015088796615600586,
-0.12175408005714417,
0.005841176491230726,
-0.05089334398508072,
0.025120412930846214,
0.03615877404808998,
0.062230631709098816,
0.011688562110066414,
-0.002578340470790863,
0.010358904488384724,
-0.034892451018095016,
-0.007238453719764948,
0.03553371503949165,
0.02873365208506584,
-0.004904655274003744,
-0.020659232512116432,
-0.0030340556986629963,
0.030225269496440887,
-0.048509154468774796,
-0.021627895534038544,
0.002723508281633258,
0.029088057577610016,
-0.026029251515865326,
0.05150839686393738,
0.012067386880517006,
0.07064146548509598,
0.07212188839912415,
-0.02337728627026081,
0.03593113645911217,
0.0482228621840477,
-0.06457871198654175,
-0.037853971123695374,
-0.01983817107975483,
0.0031654618214815855,
-0.15966710448265076,
-0.015545297414064407,
0.06029248610138893,
0.023365560919046402,
-0.06597837805747986,
-0.02784692496061325,
0.007665879093110561,
0.005005628801882267,
-0.019128339365124702,
0.02412702888250351,
0.006075392942875624,
0.016110587865114212,
0.06757339835166931,
-0.005985446274280548,
-0.07408870756626129,
-0.03614259138703346,
-0.0014816044131293893,
-0.01681014522910118,
0.07779288291931152,
0.045707426965236664,
-0.029781972989439964,
-0.0014966385206207633,
-0.0450860969722271,
-0.020355893298983574,
-0.0004977950011380017,
-0.062257587909698486,
0.023362161591649055,
0.020916754379868507,
-0.034489937126636505,
0.0686693862080574,
0.031064147129654884,
0.08726590871810913,
0.04699775204062462,
-0.010517183691263199,
0.02448701113462448,
0.031026363372802734,
-0.06421896815299988,
0.48491400480270386,
0.018478358164429665,
0.013995209708809853,
0.03244176134467125,
0.024795040488243103,
-0.0031470453832298517,
-0.005420576315373182,
-0.016008786857128143,
-0.007479134481400251,
0.03986251726746559,
0.014874393120408058,
0.0036093206144869328,
-0.006958292797207832,
0.0316598117351532,
0.009301499463617802,
0.040598295629024506,
-0.01385018415749073,
0.012293156236410141,
0.04303858429193497,
-0.011219847947359085,
-0.0022851908579468727,
0.006166071631014347,
0.009288690984249115,
0.02166074328124523,
0.012017171829938889,
0.024282414466142654,
-0.02765864133834839,
0.040336139500141144,
0.07986993342638016,
0.018915727734565735,
-0.0654473677277565,
0.015387746505439281,
-0.024849683046340942,
-0.028463253751397133,
0.011725340969860554,
-0.02933184802532196,
-0.039542343467473984,
-0.009308099746704102,
0.026916075497865677,
0.030304118990898132,
0.02446541003882885,
0.05601413547992706,
-0.06682796031236649,
-0.026768352836370468,
-0.08144102245569229,
-0.03515178710222244,
0.08642032742500305,
0.08263471722602844,
-0.01368764042854309,
0.011237064376473427,
-0.0026563212741166353,
-0.0015705213882029057,
0.01502544991672039,
0.016938064247369766,
-0.003034960012882948,
-0.019223876297473907,
-0.011215722188353539,
0.013473700731992722,
0.004401776473969221,
-0.03233523294329643,
-0.041915107518434525,
0.012884825468063354,
-0.02016962319612503,
-0.034713856875896454,
0.06745822727680206,
0.009154417552053928,
0.015858203172683716,
-0.051958855241537094,
0.005432456731796265,
0.013956653885543346,
0.013076163828372955,
0.06175469979643822,
-0.02750963717699051,
-0.031201614066958427,
0.07056420296430588,
0.04816494137048721,
0.0022334735840559006,
0.014878667891025543,
0.008914594538509846,
-0.028222734108567238,
0.026816409081220627,
0.002095866948366165,
-0.027204692363739014,
-0.059817440807819366,
-0.008720646612346172,
-0.03281540051102638,
0.011264915578067303,
-0.030504241585731506,
0.023203840479254723,
0.05988570675253868,
0.012305931188166142,
0.012436461634933949,
0.014398868195712566,
-0.0629177913069725,
-0.007172006648033857,
0.01317509263753891,
-0.018133312463760376,
-0.0316312275826931,
-0.003391037695109844,
0.03929954022169113,
-0.015485910698771477,
-0.041680630296468735,
-0.04529323801398277,
0.027735553681850433,
-0.003933611325919628,
-0.0017261201282963157,
0.004376530647277832,
-0.015158571302890778,
-0.0270308256149292,
0.030324330553412437,
0.019464854151010513,
0.006059726700186729,
-0.026868481189012527,
0.06580446660518646,
0.0799427255988121,
0.04306746646761894,
0.04646850377321243,
0.004872703924775124,
-0.029274920001626015,
0.057529669255018234,
0.004770309664309025,
0.07067938894033432,
-0.010696113109588623,
-0.030102796852588654,
-0.3292678892612457,
-0.05889450013637543,
-0.015141881071031094,
-0.053585927933454514,
-0.0324275940656662,
-0.02883775904774666,
0.00787146296352148,
-0.022831497713923454,
-0.052598439157009125,
-0.02200794592499733,
0.06634623557329178,
-0.023271672427654266,
-0.026907585561275482,
0.043774813413619995,
0.007424511946737766,
0.011122729629278183,
0.0034052676055580378,
-0.024577641859650612,
-0.07772128283977509,
-0.01635243371129036,
0.022970981895923615,
0.04962700605392456,
0.037307385355234146,
0.01409071683883667,
0.07599199563264847,
-0.07039070129394531,
0.13333457708358765,
-0.12534990906715393,
-0.052904196083545685,
-0.019895099103450775,
-0.015389583073556423,
0.03383494168519974,
-0.04535473510622978,
-0.09249501675367355,
0.021799085661768913,
-0.036722347140312195,
0.017185639590024948,
-0.01911613717675209,
-0.05190839618444443,
0.01967080868780613,
-0.05715493857860565,
0.016425209119915962,
-0.00402255542576313,
0.06257608532905579,
-0.12142626196146011,
-0.025248749181628227,
0.0020753571297973394,
-0.02430407516658306,
-0.07378458231687546,
0.004477333277463913,
0.009572340175509453,
-0.0017178056295961142,
0.012026440352201462,
0.011607220396399498,
0.006674963980913162,
-0.02294223941862583,
-0.08728553354740143,
0.04924469068646431,
-0.04496096074581146,
0.005288904067128897,
-0.027072718366980553,
0.0006044759647920728,
0.07924913614988327,
0.0034602119121700525,
0.04611539468169212,
-0.04775521531701088,
0.004025049041956663,
-0.003690693760290742,
-0.0151666309684515,
0.0068681687116622925,
0.027365030720829964,
0.08302853256464005,
-0.04413440078496933,
-0.013116960413753986,
0.011456172913312912,
-0.018622027710080147,
-0.020495891571044922,
0.007082674186676741,
0.04501840099692345,
0.011015117168426514,
-0.006844390649348497,
-0.04232705384492874,
0.03958494961261749,
0.09089505672454834,
-0.006003394722938538,
-0.04916379228234291,
-0.03004823811352253,
-0.00997839029878378,
0.007171559613198042,
0.009646951220929623,
0.0007316984701901674,
0.03147802874445915,
-0.0839962288737297,
-0.03600100427865982,
0.0809645801782608,
-0.0017478553345426917,
-0.26822352409362793,
-0.006038231775164604,
-0.022445878013968468,
0.02235340140759945,
0.01633152924478054,
-0.018169449642300606,
-0.03142765536904335,
0.03856164216995239,
-0.016930745914578438,
0.032624706625938416,
0.043122828006744385,
0.009324316866695881,
0.0008113553049042821,
-0.026588555425405502,
0.03901682794094086,
-0.02677552029490471,
0.0016980774234980345,
0.0053416891023516655,
-0.003159954445436597,
-0.07515642046928406,
0.03385355323553085,
0.015469033271074295,
0.1067628562450409,
-0.04146857187151909,
-0.0029380377382040024,
0.08750969171524048,
-0.017040982842445374,
-0.008604013361036777,
-0.0179918073117733,
-0.051388196647167206,
0.004905040841549635,
-0.0021229952108114958,
0.07700957357883453,
-0.027749547734856606,
0.03542457893490791,
-0.016283662989735603,
-0.04313769191503525,
0.01052983570843935,
-0.0016588135622441769,
0.007529157213866711,
-0.022722192108631134,
0.017273591831326485,
0.013753412291407585,
0.01084510050714016,
0.08167147636413574,
-0.037856269627809525,
0.010917620733380318,
-0.022850655019283295,
0.015739422291517258,
0.030298421159386635,
-0.04257378354668617,
-0.0204524714499712,
0.035911671817302704,
0.03530184552073479,
0.013897786848247051,
0.011679803021252155,
-0.031707514077425,
-0.0015508707147091627,
0.06002681329846382,
-0.07742388546466827,
-0.019773900508880615,
0.005900310352444649,
-0.007773428224027157,
0.0285533145070076,
-0.015048534609377384
] |
PMID:27119 | [On postoperative azotemia in geriatric surgery: significance, risk groups, possibilities of prophylaxis (author's transl)]. | 1032 Interventions on geriatric patients beeing more than 70 years old between 1966 and 1975 demonstrate the significance of azotemia in the postoperativ stage of the disease appears as well as its dependence upon prehospital factors, type and localisation also on the extension and duration on the intervention in each single case. Since it is hardly possible to exert an influence on the preliminary stress due to the old age and on the dysfunction determined by involution of the parenchymatose organs important for the course of the postoperative stage, a particular emphasis should be laid upon the prophylactic aspekt to the purpose of an appropriate screening against vegetativ-visceral, renally effective shock reflexes (hepatorenal syndrome) and on the other, usually extra-renal factors (PH 203R, splanchnic block). Further possibilities: considerate elimination of pain and general anesthesia, purpuseful and quick operative technique, improvement of the apparatus and instruments, after-treatment specific for the given case so as one consisting of a prophylaxis against pneumonia through a rapid reactivation of the operated person. However, regardless to all the professional advance made in this field, the primary human approach to the aged patient and to his life expectancy should be made due allowance for. | [On postoperative azotemia in geriatric surgery: significance, risk groups, possibilities of prophylaxis (author's transl)]. 1032 Interventions on geriatric patients beeing more than 70 years old between 1966 and 1975 demonstrate the significance of azotemia in the postoperativ stage of the disease appears as well as its dependence upon prehospital factors, type and localisation also on the extension and duration on the intervention in each single case. Since it is hardly possible to exert an influence on the preliminary stress due to the old age and on the dysfunction determined by involution of the parenchymatose organs important for the course of the postoperative stage, a particular emphasis should be laid upon the prophylactic aspekt to the purpose of an appropriate screening against vegetativ-visceral, renally effective shock reflexes (hepatorenal syndrome) and on the other, usually extra-renal factors (PH 203R, splanchnic block). Further possibilities: considerate elimination of pain and general anesthesia, purpuseful and quick operative technique, improvement of the apparatus and instruments, after-treatment specific for the given case so as one consisting of a prophylaxis against pneumonia through a rapid reactivation of the operated person. However, regardless to all the professional advance made in this field, the primary human approach to the aged patient and to his life expectancy should be made due allowance for. | [
-0.04436513036489487,
0.05278254672884941,
-0.00944613665342331,
0.008139061741530895,
0.00870478618890047,
0.015914151445031166,
0.08731948584318161,
0.07906084507703781,
-0.07740233838558197,
-0.01890300028026104,
-0.0057442388497292995,
0.003830462461337447,
-0.03302987664937973,
0.02541620098054409,
-0.07745091617107391,
0.029183924198150635,
0.01860990561544895,
0.016612133011221886,
-0.05533067509531975,
0.02078830823302269,
-0.0301774013787508,
0.044194381684064865,
0.03833044692873955,
-0.0009194955346174538,
0.009493314661085606,
0.05922817438840866,
-0.052648529410362244,
-0.030153585597872734,
-0.03362493962049484,
-0.14092496037483215,
-0.01373253669589758,
-0.07743457704782486,
0.021410899236798286,
-0.048437826335430145,
-0.037116069346666336,
0.034484751522541046,
-0.026702841743826866,
0.05120237171649933,
-0.07110227644443512,
0.006382344756275415,
0.0444650873541832,
-0.011788959614932537,
-0.025388425216078758,
-0.01234382949769497,
-0.02283363603055477,
-0.0025772186927497387,
-0.019249556586146355,
-0.013120949268341064,
0.07601458579301834,
0.06309161335229874,
-0.014709481038153172,
-0.009249700233340263,
0.011556597426533699,
0.1191297397017479,
0.03170256316661835,
-0.07857207953929901,
-0.0023298391606658697,
0.025605961680412292,
-0.07314319163560867,
0.03280119225382805,
-0.00726913521066308,
-0.024459367617964745,
-0.13343419134616852,
0.014523405581712723,
-0.011364550329744816,
-0.013370922766625881,
-0.00732706394046545,
-0.040535952895879745,
0.08184781670570374,
-0.04016229510307312,
-0.0296283308416605,
0.044217299669981,
-0.051608577370643616,
0.0636095330119133,
-0.021329035982489586,
-0.012483388185501099,
0.007950958795845509,
-0.0012665166286751628,
-0.0018067150376737118,
0.01959744654595852,
0.03164617344737053,
0.016032764688134193,
-0.057293690741062164,
-0.00013600935926660895,
0.0007900684140622616,
-0.049266085028648376,
0.013465387746691704,
-0.0371212437748909,
-0.041406355798244476,
-0.04295497015118599,
-0.002787418430671096,
0.038361839950084686,
-0.015065066516399384,
0.02377326413989067,
-0.035969894379377365,
-0.024348191916942596,
-0.007348611485213041,
0.025631988421082497,
-0.00820099376142025,
0.3845129609107971,
0.00035992800258100033,
0.03504474088549614,
-0.019924968481063843,
0.012433844618499279,
0.00904894433915615,
-0.049392879009246826,
-0.004532543011009693,
0.022965973243117332,
0.0146505581215024,
0.058271415531635284,
0.04411368444561958,
0.016870109364390373,
0.05485226586461067,
0.0023921015672385693,
-0.024952411651611328,
0.0990828275680542,
0.07768573611974716,
0.10947097837924957,
-0.023197723552584648,
-0.006494747940450907,
0.015728112310171127,
-0.025043966248631477,
0.011946337297558784,
-0.03393949940800667,
0.049524035304784775,
0.018738407641649246,
-0.015911122784018517,
0.06285107880830765,
-0.013930964283645153,
0.010169066488742828,
0.046065982431173325,
0.016684697940945625,
0.007629673462361097,
0.016232218593358994,
0.0008665036875754595,
0.005865062586963177,
0.06706659495830536,
-0.0238723773509264,
0.026996804401278496,
-0.027463378384709358,
-0.002389059867709875,
-0.096107617020607,
-0.03134860098361969,
-0.020675765350461006,
-0.019044075161218643,
0.08659172803163528,
0.01105955708771944,
0.015461137518286705,
0.023673206567764282,
0.0019812644459307194,
-0.013046291656792164,
0.06347393244504929,
-0.004501190036535263,
0.04646332189440727,
-0.0191368255764246,
-0.005981455557048321,
-0.02420244924724102,
0.023435965180397034,
-0.045141905546188354,
-0.023967528715729713,
-0.0980868935585022,
-0.06743519753217697,
-0.030573787167668343,
0.06426797062158585,
0.0162560623139143,
-0.013760463334619999,
-0.04933137074112892,
-0.0106666199862957,
0.01670956052839756,
0.06447292864322662,
0.05615716800093651,
-0.03364396467804909,
-0.00958253163844347,
0.02336808666586876,
0.03808726370334625,
0.04844493418931961,
0.017252173274755478,
0.007603569887578487,
-0.017492959275841713,
0.029310323297977448,
0.04418646916747093,
0.0263934638351202,
-0.013602552935481071,
0.01203666441142559,
-0.030935000628232956,
0.005943008698523045,
-0.02818731777369976,
0.035982947796583176,
0.038627658039331436,
0.006456953007727861,
0.013469498604536057,
-0.02543295919895172,
-0.07608732581138611,
0.007949556224048138,
0.06120280921459198,
-0.04539407417178154,
-0.0667499452829361,
0.040112849324941635,
-0.011904792860150337,
-0.030498337000608444,
0.03340206295251846,
-0.04535377398133278,
-0.04479571804404259,
0.037616852670907974,
-0.0532841719686985,
0.000017171536455862224,
0.018159212544560432,
-0.016724485903978348,
0.01519523374736309,
-0.02476324327290058,
-0.016674797981977463,
0.039786990731954575,
0.044911619275808334,
0.07702730596065521,
-0.0213377196341753,
0.09352218359708786,
-0.04383483901619911,
0.011921623721718788,
-0.0016096130711957812,
0.008985702879726887,
0.08264066278934479,
0.01150398887693882,
-0.000891411560587585,
-0.3545628488063812,
0.00912450347095728,
0.010709431953728199,
-0.05455220490694046,
0.025901097804307938,
-0.022971687838435173,
-0.016912709921598434,
-0.026675019413232803,
-0.026464777067303658,
-0.06639230251312256,
0.06550796329975128,
0.04280218854546547,
-0.03282665088772774,
-0.003944853786379099,
0.023928243666887283,
0.006980241741985083,
0.027825510129332542,
-0.044982586055994034,
-0.034110043197870255,
0.0025575205218046904,
0.0016026217490434647,
0.0006360844126902521,
-0.007576105184853077,
0.03332281857728958,
0.04366698116064072,
-0.06748958677053452,
0.13104800879955292,
-0.06191015616059303,
-0.07803095132112503,
-0.06116916239261627,
0.008426561951637268,
-0.0376889742910862,
-0.06374363601207733,
-0.08447428047657013,
0.021983975544571877,
-0.05162661522626877,
0.020405394956469536,
0.01543703768402338,
-0.017864765599370003,
0.004751671105623245,
-0.018690738826990128,
0.005133525934070349,
-0.009151020087301731,
0.015079902485013008,
-0.013656869530677795,
-0.04731638357043266,
0.015819977968931198,
0.029287924990057945,
-0.0009459291468374431,
0.01778625324368477,
0.030259650200605392,
0.010719670914113522,
-0.010369292460381985,
0.0016536688199266791,
0.040955305099487305,
-0.030098287388682365,
-0.025522669777274132,
-0.0012329209130257368,
-0.05579204857349396,
-0.034560732543468475,
-0.024313809350132942,
0.06839706003665924,
0.0787888914346695,
0.009855411015450954,
-0.01718328334391117,
-0.012647254392504692,
-0.0008808007114566863,
0.012087110430002213,
0.08731962740421295,
-0.033241987228393555,
-0.02516663447022438,
0.13671709597110748,
-0.09633737057447433,
-0.0520954504609108,
0.0686582550406456,
0.021799124777317047,
0.026687337085604668,
0.0060814968310296535,
0.012825045734643936,
0.008495742455124855,
-0.007057171314954758,
-0.026739362627267838,
0.004862772300839424,
0.04150887206196785,
0.0044081201776862144,
-0.013267725706100464,
-0.04248599335551262,
0.015825163573026657,
-0.013734537176787853,
0.005850631278008223,
-0.019979311153292656,
-0.008346345275640488,
-0.04128936305642128,
-0.0021789968013763428,
0.05861574411392212,
-0.005902040749788284,
-0.275321364402771,
0.04641617462038994,
-0.030365008860826492,
0.006975173484534025,
0.01443355344235897,
-0.012217313051223755,
-0.01160047110170126,
-0.09464617818593979,
-0.023341482505202293,
0.004471292719244957,
0.011524464935064316,
0.02766590565443039,
0.07871511578559875,
0.024080809205770493,
-0.005989168770611286,
0.007347511127591133,
0.04138559475541115,
0.02123970165848732,
0.005313873291015625,
-0.03830535337328911,
0.051504895091056824,
-0.025145595893263817,
0.10503645986318588,
-0.027012018486857414,
-0.007615774404257536,
0.08697192370891571,
0.06368967890739441,
0.021181009709835052,
0.012544694356620312,
0.002785217482596636,
-0.004286380019038916,
0.0052049471996724606,
0.04136097803711891,
-0.01957261748611927,
0.0748637244105339,
0.010756743140518665,
-0.07127388566732407,
0.06965125352144241,
-0.0002121276775142178,
0.017954478040337563,
-0.038492992520332336,
-0.013490774668753147,
0.008203815668821335,
-0.020897675305604935,
0.0932365283370018,
-0.05904815346002579,
-0.012961078435182571,
-0.07568196207284927,
-0.04548012092709541,
-0.007973024621605873,
-0.013657855801284313,
0.0036008639726787806,
0.0177975594997406,
0.016902338713407516,
0.010178927332162857,
-0.042614758014678955,
-0.007527385838329792,
-0.04358631744980812,
0.02387280948460102,
-0.0057658851146698,
-0.02220032550394535,
-0.006007719784975052,
-0.03408726304769516,
0.08016800880432129,
-0.017650261521339417
] |
PMID:27120 | Ketamine sequelae. Evaluation of the ability of various premedicants to attenuate its psychic actions. | The ability of a number of drugs to abolish the emergence delirium and unpleasant dreams which follow anaesthesia induced with 2 mg/kg ketamine was studied. These included three benzodiazepines, droperidol and 'neurolept' combinations and four commonly-used premedicants. When given intravenously 10 min before induction of anaesthesia flunitrazepam and lorazepam gave best results. In a subsequent study, these two benzodiazepines and diazepam were given intravenously 30-40 min before induction of anaesthesia. There was no doubt that 4 mg lorazepam gave the greatest protection and is worthy of further study in this respect. | Ketamine sequelae. Evaluation of the ability of various premedicants to attenuate its psychic actions. The ability of a number of drugs to abolish the emergence delirium and unpleasant dreams which follow anaesthesia induced with 2 mg/kg ketamine was studied. These included three benzodiazepines, droperidol and 'neurolept' combinations and four commonly-used premedicants. When given intravenously 10 min before induction of anaesthesia flunitrazepam and lorazepam gave best results. In a subsequent study, these two benzodiazepines and diazepam were given intravenously 30-40 min before induction of anaesthesia. There was no doubt that 4 mg lorazepam gave the greatest protection and is worthy of further study in this respect. | [
-0.04841389134526253,
0.002255102153867483,
0.031690772622823715,
0.022450340911746025,
0.0055771335028111935,
0.04856984317302704,
0.06007450073957443,
-0.02021275833249092,
0.024500373750925064,
-0.07888123393058777,
-0.03376728296279907,
-0.003990548197180033,
-0.009317837655544281,
0.020441655069589615,
-0.02606985531747341,
-0.015879902988672256,
0.027936287224292755,
-0.10114240646362305,
-0.061930522322654724,
0.0047187176533043385,
-0.003281249199062586,
0.013589844107627869,
0.032912932336330414,
0.014670230448246002,
0.013719502836465836,
-0.027048828080296516,
0.06636103242635727,
0.0008835464250296354,
-0.0248482096940279,
-0.1597299426794052,
0.037093017250299454,
-0.005459710024297237,
0.060251615941524506,
-0.06615771353244781,
-0.05406560003757477,
0.021491628140211105,
-0.06583131104707718,
0.10619346797466278,
-0.04519805684685707,
-0.04805462807416916,
0.08784633874893188,
0.03280750289559364,
0.0026277960278093815,
-0.056515034288167953,
0.026364272460341454,
-0.014156865887343884,
0.011562523432075977,
-0.07094091176986694,
0.13205446302890778,
-0.03171812370419502,
0.05865105614066124,
-0.03047371655702591,
0.04372309520840645,
0.06826261430978775,
0.016047658398747444,
-0.04904341325163841,
-0.06779985874891281,
-0.031772203743457794,
-0.011617856100201607,
0.04021923989057541,
-0.027882592752575874,
0.04441455751657486,
-0.1156008243560791,
0.013531098142266273,
-0.03656569495797157,
-0.0010331888915970922,
0.023786429315805435,
-0.06265908479690552,
0.0010324274189770222,
0.10552345216274261,
-0.009341428987681866,
0.00006974426651140675,
-0.01210014522075653,
0.010721704922616482,
0.043171703815460205,
-0.0001345566415693611,
0.022603608667850494,
0.005974121391773224,
-0.023308776319026947,
-0.07535543292760849,
0.023776570335030556,
-0.01473538763821125,
-0.004647711757570505,
-0.058019597083330154,
0.01638787053525448,
-0.0033960151486098766,
0.09164232760667801,
-0.028158016502857208,
-0.014660371467471123,
0.013476607389748096,
0.03154706582427025,
0.011661761440336704,
0.011325333267450333,
-0.0356871671974659,
-0.004824709612876177,
-0.03728218376636505,
-0.021206414327025414,
0.007922453805804253,
0.006873968988656998,
0.35177257657051086,
0.02139268070459366,
0.010534768924117088,
-0.05477719381451607,
-0.04619527608156204,
-0.007702965755015612,
-0.029531722888350487,
0.046419233083724976,
-0.014394566416740417,
0.0345890074968338,
0.0013174497289583087,
0.04398326203227043,
0.006690836511552334,
0.07858322560787201,
0.0268852561712265,
-0.06678583472967148,
0.022485265508294106,
0.09038519859313965,
-0.01592090167105198,
0.027800092473626137,
0.019170917570590973,
-0.02531149983406067,
-0.003054971108213067,
0.06446734815835953,
-0.020944025367498398,
0.05056134983897209,
-0.05152621120214462,
-0.054389916360378265,
0.0911756306886673,
-0.006664813030511141,
-0.01566285453736782,
-0.017187239602208138,
-0.0004165960708633065,
0.07162725925445557,
0.03948942944407463,
0.032453905791044235,
-0.0020306177902966738,
0.05010552331805229,
-0.06333020329475403,
0.043034352362155914,
-0.03800022602081299,
0.038492485880851746,
-0.08556588739156723,
0.04954835772514343,
-0.0641586184501648,
-0.062032852321863174,
0.07622281461954117,
0.000957405602093786,
-0.025287779048085213,
-0.004534824285656214,
0.010600965470075607,
-0.04513998702168465,
-0.015135390684008598,
-0.01740085333585739,
0.03489212319254875,
0.03859680891036987,
0.029657607898116112,
0.030529258772730827,
0.029414119198918343,
-0.04756242036819458,
0.05570372939109802,
-0.04929108917713165,
-0.016529491171240807,
0.007707624230533838,
0.06691595166921616,
-0.05561399459838867,
0.04337967187166214,
-0.028766268864274025,
0.011053552851080894,
-0.025278614833950996,
0.02379058673977852,
-0.0014765601372346282,
0.01442018710076809,
-0.0029351538978517056,
-0.00018734262266661972,
0.01698245480656624,
-0.022127525880932808,
0.03792129084467888,
-0.002835238119587302,
0.052080973982810974,
-0.0123794786632061,
0.03824102506041527,
-0.014038517139852047,
-0.0375988706946373,
0.04387734457850456,
-0.006954128388315439,
-0.006880511064082384,
-0.04244215041399002,
0.04809800907969475,
0.02561059221625328,
0.0050109499134123325,
-0.03770434856414795,
0.027989869937300682,
-0.07422786951065063,
-0.01971127837896347,
-0.02890811860561371,
-0.03889404237270355,
-0.05254047363996506,
-0.002891280222684145,
0.01669972576200962,
-0.04116363823413849,
0.07261164486408234,
0.03369351103901863,
0.010941839776933193,
0.0480271652340889,
0.025920681655406952,
0.045972708612680435,
0.007782111410051584,
0.026515347883105278,
0.08569567650556564,
-0.020425522699952126,
-0.014335183426737785,
0.04894289746880531,
0.04254218935966492,
0.009192317724227905,
-0.0359838604927063,
0.051024358719587326,
-0.09420847147703171,
0.02729179337620735,
0.005224204156547785,
0.0292955469340086,
0.0833817794919014,
-0.04614861309528351,
0.04771919175982475,
-0.30777570605278015,
0.022895310074090958,
0.03077314794063568,
-0.04024036228656769,
0.08407814800739288,
-0.06510628759860992,
0.052198175340890884,
-0.05258682370185852,
-0.08333805203437805,
-0.05061497539281845,
-0.03673402592539787,
-0.031034158542752266,
-0.027854643762111664,
0.047747902572155,
0.024132097139954567,
0.04132827743887901,
0.028122354298830032,
-0.06123849377036095,
0.012791304849088192,
0.014764823950827122,
0.01706346869468689,
0.032372429966926575,
-0.015775298699736595,
-0.013250867836177349,
0.019977007061243057,
0.02136194333434105,
0.056840680539608,
-0.012191363610327244,
-0.0017967851599678397,
-0.07845605164766312,
-0.02523929625749588,
0.10631310939788818,
-0.0028759469278156757,
-0.08786336332559586,
0.011367250233888626,
-0.0390227809548378,
0.02136220410466194,
-0.004346796777099371,
0.04487328603863716,
0.0012331075267866254,
-0.03753446042537689,
0.049625128507614136,
-0.009910507127642632,
-0.06198102608323097,
-0.10110162943601608,
-0.02580156736075878,
-0.06129659712314606,
0.0044103660620749,
-0.006528215948492289,
-0.009206848219037056,
0.004696908872574568,
0.022474123165011406,
-0.03448858484625816,
-0.03926035016775131,
0.03301134705543518,
-0.012635333463549614,
-0.1443575769662857,
0.012708688154816628,
-0.026989014819264412,
-0.01596626080572605,
0.010403798893094063,
0.013317640870809555,
-0.006928471848368645,
-0.044563889503479004,
-0.03455471992492676,
0.005693948827683926,
0.02628488466143608,
0.014984168112277985,
0.04733247682452202,
0.01572144776582718,
-0.03223603218793869,
0.12163782119750977,
-0.04517927020788193,
-0.015618741512298584,
0.11738165467977524,
0.016223052516579628,
-0.03973554074764252,
0.01841537281870842,
-0.04776537045836449,
0.031238151714205742,
-0.044598523527383804,
-0.009956961497664452,
-0.001575722941197455,
0.05128289759159088,
-0.04413395747542381,
-0.025886883959174156,
0.026799077168107033,
-0.0034774476662278175,
0.03587573766708374,
0.018639087677001953,
0.002623771782964468,
0.03767668455839157,
0.0052687665447592735,
0.0045077502727508545,
0.02378031425178051,
0.0012142494088038802,
-0.2209402322769165,
-0.01156076230108738,
0.026781991124153137,
0.07721516489982605,
0.025046806782484055,
0.006903990171849728,
0.03202211484313011,
-0.053721487522125244,
-0.02120569534599781,
-0.040068790316581726,
-0.03983040526509285,
-0.035797446966171265,
0.0037817140109837055,
0.01604936458170414,
-0.010067195631563663,
0.03010302223265171,
0.04408309981226921,
-0.03994736075401306,
-0.0036752396263182163,
-0.057385269552469254,
0.0091558787971735,
0.012596710585057735,
0.1267036348581314,
0.05786088854074478,
-0.02326582744717598,
0.07244479656219482,
0.05316626653075218,
0.020387716591358185,
-0.015827778726816177,
0.061107032001018524,
0.0013750094221904874,
0.005356152541935444,
0.08324132114648819,
-0.01988493837416172,
0.05228709802031517,
-0.04354593902826309,
-0.11705264449119568,
0.00733473664149642,
0.02651035040616989,
-0.01192929595708847,
-0.03357318416237831,
0.002824336988851428,
0.027276907116174698,
-0.048514846712350845,
0.09505058825016022,
-0.04446505382657051,
-0.03562433645129204,
-0.09363330900669098,
-0.057223159819841385,
-0.01907932199537754,
-0.028742820024490356,
-0.04159357771277428,
0.027333278208971024,
0.02027636021375656,
0.025742290541529655,
-0.010051549412310123,
0.001730077201500535,
0.024593988433480263,
0.02650585025548935,
-0.0007083850796334445,
-0.0500042550265789,
-0.03651167079806328,
-0.006243916694074869,
0.03302853927016258,
0.002324239816516638
] |
PMID:27121 | Ketamine-iorazepam. Attenuation of psychic sequelae of ketamine by lorazepam. | The intravenous, intramuscular and oral administration of 4 mg lorazepam given to adults will effectively reduce the psychic sequelae of ketamine to acceptable limits. | Ketamine-iorazepam. Attenuation of psychic sequelae of ketamine by lorazepam. The intravenous, intramuscular and oral administration of 4 mg lorazepam given to adults will effectively reduce the psychic sequelae of ketamine to acceptable limits. | [
0.007974210195243359,
-0.010773601941764355,
0.0058430773206055164,
0.02215634100139141,
-0.0060108499601483345,
-0.01789473369717598,
0.05514043569564819,
-0.01694941706955433,
-0.019119558855891228,
-0.0800011083483696,
0.0075837974436581135,
-0.029645374044775963,
-0.004800653085112572,
-0.01977212354540825,
0.009855973534286022,
-0.004971684422343969,
0.028537912294268608,
-0.039036497473716736,
-0.07461559027433395,
0.03634587675333023,
0.0010326245101168752,
0.02783898450434208,
0.03782206401228905,
0.023010175675153732,
-0.02993549406528473,
0.006635214202105999,
-0.014430704526603222,
-0.02107054367661476,
-0.057246651500463486,
-0.12223811447620392,
0.05906665325164795,
-0.0032025687396526337,
-0.009155556559562683,
-0.0300659891217947,
-0.07985483855009079,
0.013274649158120155,
-0.05105997249484062,
0.04081866145133972,
-0.01088989432901144,
0.015532447956502438,
0.13251811265945435,
0.04585681110620499,
0.010463648475706577,
-0.03855690732598305,
0.04177681729197502,
-0.017045026645064354,
-0.017424216493964195,
-0.03037095256149769,
0.11566563695669174,
-0.02851351909339428,
0.01090318989008665,
-0.039820604026317596,
0.043039269745349884,
0.09369957447052002,
0.01795417070388794,
0.005190156400203705,
-0.01192354504019022,
0.0031487462110817432,
0.025421515107154846,
0.07002764195203781,
-0.05704667046666145,
0.038063015788793564,
-0.10979320108890533,
0.03290748968720436,
-0.039226800203323364,
0.006296705920249224,
-0.005973479710519314,
-0.056658949702978134,
0.004817702807486057,
0.09669049084186554,
-0.02648717351257801,
-0.03183559700846672,
-0.0067177582532167435,
0.06106621026992798,
0.037517208606004715,
-0.022421447560191154,
0.06296442449092865,
0.01196023914963007,
-0.028614118695259094,
-0.050786517560482025,
-0.022322170436382294,
-0.009890045039355755,
-0.0035089252050966024,
-0.07723068445920944,
-0.010293022729456425,
0.002762444084510207,
0.04635201394557953,
-0.05649178847670555,
0.01794968545436859,
0.0075385780073702335,
-0.029039008542895317,
-0.017618736252188683,
-0.006817850284278393,
-0.037337470799684525,
0.02324383147060871,
-0.05282123386859894,
0.007474928162992001,
-0.015773680061101913,
0.0022568602580577135,
0.32236573100090027,
0.013655968010425568,
0.001193651114590466,
-0.036647845059633255,
-0.017344098538160324,
0.000397802417865023,
-0.07001373916864395,
0.07247288525104523,
-0.012362995184957981,
0.0340731255710125,
-0.005010044202208519,
0.020192759111523628,
0.025929786264896393,
0.07211184501647949,
0.015168121084570885,
-0.049669452011585236,
0.07116663455963135,
0.06898293644189835,
-0.010421850718557835,
0.0679355189204216,
0.07400333136320114,
-0.029476003721356392,
-0.006002574693411589,
0.05043937638401985,
0.026374520733952522,
0.07212407141923904,
-0.005680139642208815,
-0.03459114581346512,
0.08252892643213272,
0.024082068353891373,
-0.008485693484544754,
0.00649282755330205,
-0.05988624691963196,
0.013729947619140148,
0.01179641205817461,
0.04160477593541145,
0.012819105759263039,
0.03794323280453682,
-0.0426190160214901,
0.051675379276275635,
0.028660789132118225,
0.036953963339328766,
-0.07596170902252197,
0.011098199523985386,
-0.08531797677278519,
-0.05070441588759422,
0.12207924574613571,
-0.009580258280038834,
-0.05590116232633591,
-0.03221927210688591,
0.011170151643455029,
-0.023336337879300117,
-0.005707019008696079,
-0.005638699512928724,
0.0732884481549263,
0.012299200519919395,
0.017474528402090073,
0.026739109307527542,
0.02821328304708004,
-0.05541874095797539,
0.056388743221759796,
-0.04440601170063019,
-0.060894519090652466,
-0.01284522470086813,
0.07189324498176575,
-0.08418811857700348,
0.04418456181883812,
-0.041104383766651154,
0.009481911547482014,
-0.031557347625494,
0.015345715917646885,
0.048375558108091354,
-0.0027226919773966074,
0.016005350276827812,
-0.015064291656017303,
0.04873529449105263,
-0.02401631698012352,
0.017590204253792763,
-0.0011209138901904225,
0.05635920166969299,
0.013696982525289059,
0.015719912946224213,
0.007649403531104326,
-0.04123091697692871,
0.009600765071809292,
-0.009588534012436867,
-0.043568436056375504,
-0.009591921232640743,
0.018706930801272392,
0.009300710633397102,
-0.025899169966578484,
-0.036693960428237915,
0.0190373994410038,
-0.040794696658849716,
0.015786420553922653,
-0.010343149304389954,
-0.0436672642827034,
-0.11894876509904861,
0.038552481681108475,
0.000014864860531815793,
-0.026666076853871346,
0.04007424786686897,
0.07022497802972794,
-0.05803939700126648,
0.09117234498262405,
0.024123268201947212,
0.05052296444773674,
-0.008178159594535828,
-0.008501166477799416,
0.01835438422858715,
-0.004322783090174198,
0.008862023241817951,
0.01930650882422924,
0.04920228198170662,
0.001189085771329701,
-0.021542146801948547,
0.02696589007973671,
-0.010642822831869125,
0.0003441681619733572,
0.04511341452598572,
0.013444498181343079,
0.04112287238240242,
-0.042616430670022964,
0.0038055195473134518,
-0.3215220868587494,
-0.011000806465744972,
0.06396893411874771,
-0.045564934611320496,
0.034372176975011826,
-0.12669971585273743,
0.055389661341905594,
-0.04842754825949669,
-0.04732323810458183,
-0.06096353381872177,
-0.043794386088848114,
0.023113949224352837,
-0.021941740065813065,
0.00919802114367485,
0.029359905049204826,
0.019701899960637093,
0.059529609978199005,
-0.028226139023900032,
-0.0037930866237729788,
-0.002988368971273303,
0.012896143831312656,
0.0667378380894661,
-0.026404980570077896,
0.015040963888168335,
0.0034252707846462727,
0.013701636344194412,
0.06889311969280243,
0.013123651035130024,
-0.0068609872832894325,
-0.05061447247862816,
-0.03112095408141613,
0.08470278233289719,
-0.04430466890335083,
-0.11399856209754944,
0.00229038973338902,
-0.018657812848687172,
-0.0013452621642500162,
-0.0092256935313344,
0.0074895769357681274,
-0.008699582889676094,
-0.04473569244146347,
0.07303280383348465,
-0.03623972088098526,
-0.0683719590306282,
-0.08645985275506973,
-0.030183622613549232,
-0.022407617419958115,
0.015411220490932465,
0.044722940772771835,
-0.06829672306776047,
0.035525523126125336,
-0.00490656029433012,
-0.01355902198702097,
-0.011563802137970924,
0.04347087815403938,
-0.002814327133819461,
-0.10404713451862335,
0.004132112953811884,
-0.03805706277489662,
-0.03659845516085625,
-0.05244410037994385,
0.0026076561771333218,
-0.009045947343111038,
-0.014969630166888237,
0.01034615095704794,
-0.02243904210627079,
0.05627390742301941,
0.05433941259980202,
0.05763683095574379,
0.0066049909219145775,
-0.0013665418373420835,
0.11023353040218353,
-0.061176519840955734,
-0.02565392665565014,
0.06325691193342209,
0.03264831379055977,
-0.05264119803905487,
-0.004408338572829962,
-0.10031932592391968,
0.055874258279800415,
0.01037217304110527,
0.012506665661931038,
0.07879603654146194,
0.05948193371295929,
-0.014750296249985695,
0.023696135729551315,
-0.003163732821121812,
-0.06219865381717682,
0.003965276293456554,
-0.03678091987967491,
0.011740881949663162,
0.07128873467445374,
-0.06195692718029022,
-0.027249479666352272,
0.0019187794532626867,
0.017414696514606476,
-0.23351410031318665,
0.043976206332445145,
0.0022891818080097437,
0.11575169861316681,
0.0022838234435766935,
0.035568416118621826,
0.007059995085000992,
-0.028724728152155876,
-0.030936917290091515,
-0.05861572176218033,
-0.011848315596580505,
-0.019314050674438477,
0.03306383267045021,
0.004000567831099033,
-0.015294281765818596,
0.0054584587924182415,
0.053025227040052414,
-0.06271951645612717,
0.003235308453440666,
0.0036769353318959475,
0.003415248589590192,
0.01658233441412449,
0.11388663947582245,
0.03397291153669357,
0.005571050103753805,
0.06865537166595459,
0.013752896338701248,
0.028901133686304092,
0.04605076089501381,
-0.019052591174840927,
-0.05482809245586395,
0.019038928672671318,
0.05772840976715088,
0.010019088163971901,
0.02783154509961605,
-0.05345407500863075,
-0.13456222414970398,
0.011594767682254314,
0.03910907730460167,
-0.010200247168540955,
-0.051335014402866364,
-0.02969847060739994,
0.003546070773154497,
-0.013886182568967342,
0.09444776177406311,
-0.059727028012275696,
-0.04731634259223938,
-0.11233440041542053,
-0.046810444444417953,
-0.03578747436404228,
-0.018293611705303192,
0.02944720722734928,
0.01641879417002201,
0.021039914339780807,
0.06686235964298248,
0.03529083728790283,
0.00666016386821866,
0.017724748700857162,
0.03944927081465721,
0.0393013171851635,
-0.06517910957336426,
-0.005631707143038511,
0.04286080226302147,
0.03670913726091385,
-0.009656907059252262
] |
PMID:27123 | [The solutibility of local anaesthetics in cerebrospinal fluid and the dependency of the process on the hydrogene ion concentration (author's transl)]. | Reports on in vitro precipitation of local anesthetics suggested the possibility of a damaging effect on nervous tissue with spinal anesthesia. The present assays showed that the solubility of local anesthetics in aqueous media decreases with rising pH-levels. The partial pressure of CO2 determines the pH-level of the cerebrospinal fluid, the level ranging at about 7.35 + 0.011 (95% range: 7.327-7.371). The solubilities calculated for a pH of 7.371 (at 37 degrees C) are as follows: Bupivacaine.HCl 0.83+/-0.10 mh/ml Carticaine.HCl 27 +/-2.8 mg/ml Lidocaine.HCl 24 +/-1.3 mg/ml Mepivacaine.HCl 14.8 +/- 0.2 mg/ml Tetracaine.HCl 1.4 +/- 0.12 mg/ml. | [The solutibility of local anaesthetics in cerebrospinal fluid and the dependency of the process on the hydrogene ion concentration (author's transl)]. Reports on in vitro precipitation of local anesthetics suggested the possibility of a damaging effect on nervous tissue with spinal anesthesia. The present assays showed that the solubility of local anesthetics in aqueous media decreases with rising pH-levels. The partial pressure of CO2 determines the pH-level of the cerebrospinal fluid, the level ranging at about 7.35 + 0.011 (95% range: 7.327-7.371). The solubilities calculated for a pH of 7.371 (at 37 degrees C) are as follows: Bupivacaine.HCl 0.83+/-0.10 mh/ml Carticaine.HCl 27 +/-2.8 mg/ml Lidocaine.HCl 24 +/-1.3 mg/ml Mepivacaine.HCl 14.8 +/- 0.2 mg/ml Tetracaine.HCl 1.4 +/- 0.12 mg/ml. | [
-0.01524355448782444,
-0.020211944356560707,
-0.004482115153223276,
0.015054243616759777,
-0.046573564410209656,
0.008236464112997055,
0.03438049554824829,
0.052229803055524826,
-0.04462115094065666,
-0.03763986751437187,
-0.04897753894329071,
-0.03483489900827408,
-0.036733973771333694,
0.020443571731448174,
-0.04486226290464401,
-0.03140175715088844,
0.01123836264014244,
-0.011608751490712166,
-0.05788334086537361,
-0.016110416501760483,
0.00018635307787917554,
0.019068853929638863,
-0.02426389418542385,
-0.037142205983400345,
-0.02092760242521763,
-0.013560878112912178,
0.04279983788728714,
-0.015402386896312237,
-0.013438437134027481,
-0.2381105124950409,
-0.0020489543676376343,
-0.027281932532787323,
0.06325740367174149,
-0.03601781651377678,
-0.001831082976423204,
0.0595843642950058,
-0.009967075660824776,
-0.017617862671613693,
0.004594828933477402,
0.04853235185146332,
0.019797684624791145,
0.013371244072914124,
-0.012602315284311771,
0.011324170045554638,
-0.02614649571478367,
-0.018709857016801834,
-0.007351688574999571,
0.03474899381399155,
0.15490157902240753,
0.08012206852436066,
0.046217720955610275,
0.031100120395421982,
-0.009895107708871365,
0.027566198259592056,
-0.029658319428563118,
-0.03869080916047096,
0.0054242550395429134,
0.004962476436048746,
-0.015258599072694778,
0.03999478742480278,
0.012818106450140476,
0.0883137509226799,
-0.14232851564884186,
-0.0021037827245891094,
0.014661170542240143,
-0.005054870154708624,
0.011252157390117645,
-0.004287282004952431,
0.02819220721721649,
0.05775199085474014,
-0.04236704856157303,
0.032907988876104355,
0.02251664362847805,
0.019793912768363953,
0.05256618186831474,
-0.035432782024145126,
0.005683343391865492,
0.016220198944211006,
0.018586913123726845,
-0.07383435219526291,
0.017550451681017876,
-0.04576155170798302,
-0.039221830666065216,
-0.036020997911691666,
-0.004972917027771473,
-0.007424979005008936,
0.11643687635660172,
-0.003487352980300784,
-0.028003906831145287,
0.011188039556145668,
0.004222256131470203,
-0.0417829267680645,
-0.01854461058974266,
-0.0013814449775964022,
-0.06980434060096741,
-0.018717030063271523,
0.015212626196444035,
0.018287280574440956,
-0.0029515924397855997,
0.3601089417934418,
-0.019763892516493797,
-0.009028241038322449,
-0.04511626809835434,
-0.06627347320318222,
-0.016739804297685623,
-0.011043181642889977,
0.08206585049629211,
0.0031383803579956293,
0.06793083995580673,
0.025947164744138718,
0.0009869084460660815,
0.04422219842672348,
0.015145757235586643,
-0.018642034381628036,
-0.05661309137940407,
0.07092390954494476,
0.03185170888900757,
0.033686552196741104,
0.024459386244416237,
-0.04650329053401947,
-0.056614283472299576,
-0.03528594225645065,
0.04924362152814865,
-0.006851088255643845,
0.03503048047423363,
-0.013603823259472847,
0.004141094163060188,
0.08643912523984909,
0.018649764358997345,
0.04927406087517738,
0.01294579729437828,
-0.012698743492364883,
0.0005132281221449375,
0.018745604902505875,
0.026186976581811905,
-0.02214674837887287,
0.012912900187075138,
-0.022726386785507202,
0.014401606284081936,
-0.023136870935559273,
-0.01839005947113037,
-0.060016680508852005,
-0.04930717870593071,
-0.042652279138565063,
-0.07718425244092941,
0.05806395784020424,
-0.008537841029465199,
-0.033757470548152924,
-0.04947856068611145,
-0.014682045206427574,
-0.07825454324483871,
0.05721285194158554,
-0.0421033538877964,
0.04832479730248451,
0.012673486955463886,
-0.05597260966897011,
0.0022292924113571644,
-0.01653127186000347,
-0.058589305728673935,
-0.010551665909588337,
0.01694853976368904,
-0.06863148510456085,
-0.01192555297166109,
0.12852004170417786,
-0.005339480005204678,
0.015640854835510254,
-0.031963836401700974,
-0.012597676366567612,
0.04904758930206299,
0.03261388838291168,
0.03212105110287666,
-0.019843077287077904,
0.011131996288895607,
-0.003349865321069956,
0.007960258051753044,
0.005285816267132759,
-0.0038537741638720036,
-0.0036129066720604897,
0.040710076689720154,
-0.03219711035490036,
0.043985169380903244,
0.05518638342618942,
-0.06779202073812485,
0.05659475177526474,
0.033549707382917404,
0.001369823468849063,
-0.03564518690109253,
-0.0055993846617639065,
-0.014263314194977283,
0.018148386850953102,
-0.03763844072818756,
-0.018275657668709755,
-0.10175665467977524,
0.003184201195836067,
-0.006669685710221529,
-0.01587340608239174,
-0.005967491772025824,
-0.049687616527080536,
0.006513232830911875,
0.00865279883146286,
0.08416102081537247,
0.009119488298892975,
-0.08847547322511673,
0.03897532820701599,
0.06351710110902786,
0.06751085072755814,
-0.011858307756483555,
0.0557483471930027,
0.006934885401278734,
-0.004700298421084881,
-0.010074738413095474,
0.04684078320860863,
-0.027331167832016945,
-0.0508715957403183,
-0.05647861585021019,
0.012408921495079994,
0.009912816807627678,
-0.0051590995863080025,
0.004346356261521578,
-0.01127239502966404,
0.13511307537555695,
-0.059770870953798294,
-0.003010798478499055,
-0.2790307104587555,
0.033983904868364334,
0.004429341293871403,
-0.06475178897380829,
0.09806250035762787,
-0.021667664870619774,
0.06839130818843842,
0.012816083617508411,
-0.0011606741463765502,
-0.0535300113260746,
0.05377274379134178,
0.0001843724021455273,
-0.06995859742164612,
0.05331958457827568,
0.03387190029025078,
-0.07716084271669388,
0.07547517120838165,
-0.016532551497220993,
0.05467641353607178,
-0.013148737139999866,
0.026650715619325638,
-0.032590415328741074,
-0.051067933440208435,
0.07612241804599762,
0.0440194196999073,
-0.017635073512792587,
0.08252772688865662,
-0.05515168979763985,
0.030962098389863968,
-0.058587998151779175,
0.04523805156350136,
0.03164408355951309,
-0.014548058621585369,
-0.07188066840171814,
-0.027573302388191223,
-0.01871182955801487,
0.030744386836886406,
0.04061412811279297,
-0.011699708178639412,
0.018332216888666153,
-0.05302950367331505,
0.026823215186595917,
0.014546787366271019,
-0.00993834063410759,
-0.05355093255639076,
-0.016210291534662247,
0.01644505374133587,
0.035439442843198776,
-0.05729491263628006,
-0.042147181928157806,
0.03928929194808006,
-0.005175887607038021,
-0.04826056584715843,
-0.06378050148487091,
0.038645029067993164,
-0.05429599806666374,
-0.03556527569890022,
-0.04445790499448776,
-0.059947967529296875,
-0.0317344032227993,
0.02528458461165428,
0.021478701382875443,
0.05874120444059372,
-0.05888368934392929,
-0.024551428854465485,
0.004397298209369183,
0.05333865433931351,
-0.06698572635650635,
0.08914339542388916,
0.01501809898763895,
0.03079567290842533,
0.13434971868991852,
-0.002139571588486433,
0.013423940166831017,
0.02184266597032547,
0.016914382576942444,
0.033739496022462845,
-0.00475835707038641,
-0.0220447089523077,
0.027229130268096924,
0.001359537593089044,
0.012783435173332691,
0.0644361600279808,
0.0030315739568322897,
0.041802383959293365,
-0.03528783842921257,
0.03276064619421959,
-0.03751268610358238,
0.013582774437963963,
-0.018622348085045815,
0.04167957231402397,
0.002327554626390338,
-0.01731732301414013,
-0.026608388870954514,
0.05419539287686348,
0.030826395377516747,
-0.2858239710330963,
-0.0024018618278205395,
0.030865207314491272,
-0.00411451468244195,
0.061578042805194855,
-0.05839969217777252,
0.009767410345375538,
-0.029614029452204704,
-0.022638071328401566,
0.005258696619421244,
-0.02844366803765297,
0.04684680327773094,
0.02601240761578083,
0.04713045433163643,
-0.005580510478466749,
-0.0032467509154230356,
0.0248515997081995,
-0.052746132016181946,
0.038850944489240646,
-0.05585074424743652,
0.018558746203780174,
-0.02613862045109272,
0.11258428543806076,
0.024216312915086746,
-0.03213757649064064,
0.0493004210293293,
0.011997067369520664,
0.03846457228064537,
0.013950425200164318,
0.0198790542781353,
-0.024647114798426628,
0.013112129643559456,
0.0448424257338047,
-0.02317759208381176,
0.05081624910235405,
0.008054153062403202,
-0.04694569110870361,
-0.0026225196197628975,
0.029906686395406723,
-0.02241669036448002,
0.029987921938300133,
0.011825278401374817,
-0.044720761477947235,
0.00782446376979351,
0.05627667158842087,
-0.05471740663051605,
0.013191482052206993,
-0.09367614984512329,
-0.0006487587234005332,
-0.012104296125471592,
0.012759599834680557,
-0.034706998616456985,
0.014609195291996002,
-0.003675880841910839,
-0.02038283832371235,
0.055836234241724014,
-0.01273774728178978,
-0.04736640676856041,
0.01314665749669075,
-0.030124953016638756,
-0.025712929666042328,
-0.04651474580168724,
0.02927893027663231,
0.06388893723487854,
0.05496305972337723
] |
PMID:27124 | [pH of the cerebro spinal fluid during subdural block (author's transl)]. | This study was initiated by other authors who found that bupivacaine precipitated together with spinal fluid in vitro and who assumed that during spinal anaesthesia complications might be possible. Under the influence of several local anaesthetic agents the pH of the spinal fluid and in 160 cases the precipitation was examined. There was no precipitation. The experimental conditions were nearly in vivo conditions. The results differed clearly from those in vitro. While in vitro bupivacaine precipitated from a pH higher than 7.37 in concentrations higher than 0.8 mg/ml [2], the pH of the spinal fluid in vivo was lower than 7.35 continually and the concentration of bupivacaine not higher than 0.3 mg/ml. This means that the border of bupivacaine precipitation is by far not reached in vivo and that complications therefore are not possible. | [pH of the cerebro spinal fluid during subdural block (author's transl)]. This study was initiated by other authors who found that bupivacaine precipitated together with spinal fluid in vitro and who assumed that during spinal anaesthesia complications might be possible. Under the influence of several local anaesthetic agents the pH of the spinal fluid and in 160 cases the precipitation was examined. There was no precipitation. The experimental conditions were nearly in vivo conditions. The results differed clearly from those in vitro. While in vitro bupivacaine precipitated from a pH higher than 7.37 in concentrations higher than 0.8 mg/ml [2], the pH of the spinal fluid in vivo was lower than 7.35 continually and the concentration of bupivacaine not higher than 0.3 mg/ml. This means that the border of bupivacaine precipitation is by far not reached in vivo and that complications therefore are not possible. | [
-0.035411469638347626,
-0.01511839497834444,
-0.01265623141080141,
0.009480549953877926,
0.01811789721250534,
-0.005779425147920847,
0.03223296254873276,
0.04896038770675659,
-0.010316058062016964,
-0.031666163355112076,
-0.05241694673895836,
-0.059630412608385086,
-0.01276213489472866,
0.036387793719768524,
-0.06463222950696945,
-0.036063097417354584,
-0.0062335883267223835,
-0.025373272597789764,
-0.04209616035223007,
0.017148932442069054,
-0.020215293392539024,
0.005949900485575199,
-0.03462427854537964,
-0.03022933565080166,
-0.05307021364569664,
-0.02687319926917553,
0.04239466413855553,
-0.003389188786968589,
0.01102953590452671,
-0.21501405537128448,
-0.015902884304523468,
-0.030958469957113266,
0.026175422593951225,
-0.034493107348680496,
0.0008934653596952558,
0.059365320950746536,
0.0007942033116705716,
0.017163533717393875,
0.013951933942735195,
0.031287964433431625,
0.03993070870637894,
0.03965536132454872,
-0.02550349198281765,
0.05256406590342522,
-0.01321845967322588,
0.0015147945377975702,
0.02834475226700306,
0.05083557218313217,
0.16678017377853394,
0.03338858112692833,
0.050170280039310455,
0.008066408336162567,
0.005972577724605799,
0.04729171469807625,
-0.04952489584684372,
-0.05310983210802078,
-0.0177028588950634,
-0.002635370707139373,
-0.05152145400643349,
0.06342754513025284,
-0.0008385425317101181,
0.06194484606385231,
-0.14153124392032623,
0.018090127035975456,
0.06110810115933418,
-0.028859183192253113,
-0.03490862250328064,
-0.026581643149256706,
0.07181864976882935,
0.05230925977230072,
-0.016046909615397453,
0.05049314722418785,
0.031083203852176666,
0.039286043494939804,
0.04366862028837204,
-0.024144122377038002,
0.028877632692456245,
0.04155188053846359,
-0.007287744432687759,
-0.008814522065222263,
-0.0061738635413348675,
-0.03742007911205292,
-0.04969106242060661,
0.023264510557055473,
0.003258740995079279,
-0.0510469451546669,
0.08890653401613235,
-0.05015646293759346,
-0.023732364177703857,
0.01428184099495411,
0.041799336671829224,
-0.04239686205983162,
-0.007413022685796022,
0.048428259789943695,
-0.06469479203224182,
0.013577422127127647,
0.05358521640300751,
0.028412042185664177,
0.021701203659176826,
0.3643324375152588,
-0.04749008268117905,
-0.010679654777050018,
-0.018908359110355377,
-0.02656686305999756,
-0.006856588181108236,
-0.021865585818886757,
0.040684521198272705,
0.008295389823615551,
0.020698530599474907,
0.03393782302737236,
-0.00598940858617425,
0.021541276946663857,
0.0488705150783062,
-0.045626942068338394,
-0.06782165169715881,
0.0496966727077961,
0.06235160306096077,
0.01813333109021187,
0.027584996074438095,
-0.10298968851566315,
-0.06641020625829697,
-0.03622603416442871,
0.049931857734918594,
0.002071075839921832,
0.01180636789649725,
-0.012723658233880997,
0.01001175306737423,
0.09417395293712616,
-0.00440223328769207,
0.05637911707162857,
0.007260295562446117,
-0.04643549397587776,
-0.007959638722240925,
0.02775401808321476,
0.023399123921990395,
-0.04286680743098259,
0.020614393055438995,
-0.03214523568749428,
0.025914886966347694,
-0.05927596986293793,
-0.0015702032251283526,
-0.04461739584803581,
-0.03425055369734764,
-0.057601068168878555,
-0.06090021878480911,
0.07546353340148926,
-0.022763734683394432,
-0.00869559682905674,
0.0065538021735847,
-0.020198971033096313,
-0.04515158757567406,
0.04950985684990883,
-0.03332043066620827,
0.06354481726884842,
0.036230068653821945,
-0.0716795101761818,
-0.00656103203073144,
0.03471599519252777,
-0.012721379287540913,
-0.027133392170071602,
0.012277213856577873,
-0.06540236622095108,
-0.006617202423512936,
0.13816535472869873,
-0.03160971403121948,
0.049389101564884186,
-0.0022989376448094845,
0.008766060695052147,
0.02172408066689968,
-0.009103118441998959,
-0.0027963449247181416,
-0.00402357941493392,
0.014796510338783264,
-0.04877778887748718,
0.01819777488708496,
0.0030074501410126686,
-0.019427938386797905,
-0.004470255691558123,
0.017545677721500397,
-0.03775234892964363,
0.0489942841231823,
0.02844800427556038,
-0.03928111493587494,
0.03196180239319801,
0.02125641331076622,
-0.05099670588970184,
-0.031346023082733154,
0.03142334893345833,
0.0007237098179757595,
-0.014372867532074451,
-0.012983739376068115,
-0.04682333767414093,
-0.11669740825891495,
0.03197986260056496,
0.018916061148047447,
-0.019186420366168022,
-0.004099150188267231,
-0.0710495114326477,
-0.027840981259942055,
-0.010898901149630547,
0.02225891686975956,
-0.00036335704498924315,
-0.08448528498411179,
0.02254454232752323,
0.021570991724729538,
0.05733765661716461,
0.012856985442340374,
0.037154920399188995,
0.022864146158099174,
0.016899684444069862,
-0.026750808581709862,
0.017075950279831886,
-0.025444243103265762,
-0.02202567085623741,
-0.06907878071069717,
0.014420521445572376,
-0.004935025703161955,
0.02138589508831501,
-0.028477147221565247,
-0.0360405370593071,
0.11333323270082474,
-0.03557134419679642,
0.06099787726998329,
-0.25819897651672363,
0.0423184297978878,
0.030889110639691353,
-0.021898983046412468,
0.110181525349617,
-0.017795482650399208,
0.08942840248346329,
0.016059203073382378,
-0.019645392894744873,
-0.11528041958808899,
0.032473959028720856,
-0.007267188746482134,
-0.07148155570030212,
0.031963951885700226,
0.03059334121644497,
-0.027721824124455452,
0.0780145600438118,
-0.020643524825572968,
0.050826966762542725,
-0.01848963089287281,
-0.020693643018603325,
-0.013621979393064976,
-0.028737913817167282,
0.07113202661275864,
0.08769826591014862,
-0.05255607143044472,
0.1108006164431572,
-0.028833743184804916,
-0.007167867850512266,
-0.0773468017578125,
0.03575641289353371,
0.01793849654495716,
0.034665729850530624,
-0.11903984844684601,
-0.01579306088387966,
-0.0066757830791175365,
0.0684821829199791,
0.05056861415505409,
-0.015625450760126114,
0.0019297716207802296,
-0.05673278495669365,
0.006813771091401577,
-0.0013667203020304441,
0.0010740699945017695,
-0.025884682312607765,
-0.021571679040789604,
-0.052632804960012436,
0.014720681123435497,
-0.08233248442411423,
-0.025628432631492615,
0.04884590208530426,
-0.04196765273809433,
-0.0409734845161438,
-0.036502618342638016,
0.021236013621091843,
-0.022053789347410202,
-0.031691037118434906,
-0.04841729626059532,
-0.031115697696805,
-0.10094467550516129,
0.02177446521818638,
0.027649329975247383,
0.061474088579416275,
-0.04965059831738472,
-0.008578114211559296,
0.03671496361494064,
0.04962272197008133,
-0.02782312221825123,
0.09456648677587509,
0.03003196232020855,
-0.025667790323495865,
0.14765411615371704,
-0.0016330110374838114,
0.03604436665773392,
0.04704757034778595,
0.01920139044523239,
0.031143793836236,
-0.01155775971710682,
-0.02159900590777397,
0.01732700504362583,
0.03882918134331703,
0.00019799615256488323,
0.03453385457396507,
-0.022706156596541405,
0.0613349974155426,
-0.02334355004131794,
-0.02524368092417717,
-0.005297701805830002,
-0.019709177315235138,
-0.015520052053034306,
-0.02183450572192669,
0.0013336354168131948,
-0.03613002598285675,
-0.02400054782629013,
0.04751015082001686,
0.02198401652276516,
-0.2585466504096985,
0.014440491795539856,
0.08361679315567017,
-0.006397560704499483,
0.037521690130233765,
-0.003814519615843892,
-0.0018986131763085723,
-0.0398038774728775,
-0.028297465294599533,
0.02782476879656315,
-0.026709752157330513,
0.027978038415312767,
0.047127615660429,
0.05307360365986824,
-0.012254019267857075,
0.050891511142253876,
0.022277960553765297,
-0.025278795510530472,
0.034389376640319824,
-0.07396069914102554,
0.01648026891052723,
-0.08471187204122543,
0.11805525422096252,
0.012228722684085369,
-0.007888446561992168,
0.05334421992301941,
-0.006443072576075792,
0.03348930552601814,
0.005698122549802065,
0.03624187782406807,
-0.048522986471652985,
0.0015283857937902212,
0.04247252270579338,
-0.0409492626786232,
0.008665474131703377,
0.01603018119931221,
-0.058110058307647705,
0.008785070851445198,
0.01914168708026409,
0.012995484285056591,
-0.009656771086156368,
0.006334085948765278,
-0.00245963828638196,
-0.017295796424150467,
0.046745866537094116,
-0.03605891764163971,
0.007364052813500166,
-0.04155195131897926,
0.0253075510263443,
-0.013348092325031757,
-0.009073141030967236,
-0.03710505738854408,
0.0064155650325119495,
-0.000580213381908834,
0.007595623843371868,
0.0023012859746813774,
0.012736408971250057,
-0.03740967437624931,
0.011807437986135483,
-0.01606893353164196,
0.018856097012758255,
-0.05285339057445526,
0.0002268389071105048,
0.04607309773564339,
-0.004568649921566248
] |
PMID:27127 | [Changes of circulation, blood gases, acid-base-and metabolism during neurolept-analgesia with and without beta-receptor blockers in electro coagulation of Gasser's ganglion (author's transl)]. | The cardiovascular, blood gas, acid-base and metabolic changes occurring during operative stress were studied in 10 elderly, metabolically healthy patients, suffering from idiopathic trigeminal neuralgia, who underwent electrocoagulation of the gasserian ganglion under neuroleptanalgesia. In a group of 12 patients undergoing the same operative procedure and anesthesthetic management, we investigated the effects of administration of a beta blocking agent on these parameters. Intraoperative rises of blood pressure and tachycardia were prevented by intravenous administration of 0.4 mg Pindolol (Visken), whereas increases in the blood glucose levels at the end of the operation were the same. The increase of glycerol, free fatty acids and ketone bodies was markedly reduced after pretreatment with the beta blocker compared with the controlgroup. In the patients without beta blocker treatment pH and standard bicarbonate at the end of the operation were lower as the result of the stimulation of ketogenesis. | [Changes of circulation, blood gases, acid-base-and metabolism during neurolept-analgesia with and without beta-receptor blockers in electro coagulation of Gasser's ganglion (author's transl)]. The cardiovascular, blood gas, acid-base and metabolic changes occurring during operative stress were studied in 10 elderly, metabolically healthy patients, suffering from idiopathic trigeminal neuralgia, who underwent electrocoagulation of the gasserian ganglion under neuroleptanalgesia. In a group of 12 patients undergoing the same operative procedure and anesthesthetic management, we investigated the effects of administration of a beta blocking agent on these parameters. Intraoperative rises of blood pressure and tachycardia were prevented by intravenous administration of 0.4 mg Pindolol (Visken), whereas increases in the blood glucose levels at the end of the operation were the same. The increase of glycerol, free fatty acids and ketone bodies was markedly reduced after pretreatment with the beta blocker compared with the controlgroup. In the patients without beta blocker treatment pH and standard bicarbonate at the end of the operation were lower as the result of the stimulation of ketogenesis. | [
-0.023993007838726044,
0.04463046416640282,
0.038296960294246674,
0.02089400589466095,
0.002519693924114108,
0.011828187853097916,
0.050330135971307755,
0.007483764551579952,
-0.05626892298460007,
-0.033522892743349075,
-0.009508797898888588,
-0.022062169387936592,
-0.028358634561300278,
0.07344022393226624,
-0.026628149673342705,
-0.0501035675406456,
0.02182072028517723,
-0.014439709484577179,
-0.03853912279009819,
-0.017982646822929382,
-0.03969092294573784,
-0.013097429648041725,
0.03751363977789879,
-0.01859661005437374,
0.009109266102313995,
0.03569827601313591,
-0.0008749229018576443,
0.012245520018041134,
-0.06499994546175003,
-0.1924804449081421,
0.04202575981616974,
-0.014185364358127117,
0.049157120287418365,
-0.018314452841877937,
-0.015329001471400261,
0.05457229167222977,
-0.056836143136024475,
0.07121696323156357,
-0.022612877190113068,
0.012177256867289543,
0.06763843446969986,
0.0034140769857913256,
-0.034002892673015594,
0.013353901915252209,
-0.012406103312969208,
-0.03320017457008362,
-0.003840409917756915,
-0.030124953016638756,
0.10698308795690536,
0.011714828200638294,
0.019205676391720772,
-0.004913861397653818,
0.0243189949542284,
0.04786507412791252,
0.011336098425090313,
-0.06089925765991211,
-0.06218451261520386,
0.017757199704647064,
0.031381335109472275,
0.016743706539273262,
-0.008306905627250671,
0.043925873935222626,
-0.16802319884300232,
0.06809192895889282,
0.04769489914178848,
-0.02588595449924469,
-0.007213464938104153,
-0.02925490215420723,
0.05304887145757675,
0.07934076339006424,
0.001457871519960463,
0.03292084485292435,
0.01800495572388172,
-0.01806747540831566,
-0.02239217236638069,
0.020164811983704567,
0.02052384801208973,
0.03485831618309021,
-0.027735009789466858,
-0.006171589717268944,
0.01647254265844822,
0.014882170595228672,
-0.05462455376982689,
-0.032193463295698166,
-0.010774185881018639,
-0.08023232966661453,
0.06639094650745392,
-0.04478222876787186,
-0.010991141200065613,
0.009322424419224262,
-0.03553999960422516,
-0.029520517215132713,
0.020888689905405045,
-0.006211694795638323,
-0.017642902210354805,
-0.039232656359672546,
-0.04251614585518837,
0.024688541889190674,
-0.04365655034780502,
0.3861362934112549,
0.005441958084702492,
0.003932065796107054,
-0.017615407705307007,
0.01516730897128582,
0.007450448349118233,
-0.0524405911564827,
0.03043426387012005,
0.016931336373090744,
-0.03685861825942993,
0.04552370309829712,
0.06242402642965317,
0.03150566294789314,
0.05967647582292557,
-0.01556259673088789,
-0.015183732844889164,
0.01636430434882641,
0.03970584273338318,
-0.009025875478982925,
0.02830391190946102,
0.025690613314509392,
-0.05031922459602356,
-0.008213196881115437,
-0.025021200999617577,
0.033017996698617935,
0.058638136833906174,
0.016284199431538582,
-0.01850667968392372,
0.0658198744058609,
0.06087478995323181,
0.0008036276558414102,
0.0431520976126194,
-0.020132120698690414,
-0.026850540190935135,
0.0482315868139267,
-0.016600901260972023,
-0.04130810871720314,
0.06883974373340607,
0.022491702809929848,
0.009867043234407902,
-0.047446150332689285,
-0.00525360694155097,
-0.030053146183490753,
0.030495038256049156,
-0.0694655030965805,
-0.029713954776525497,
0.100266233086586,
0.022482775151729584,
-0.05068562179803848,
-0.07635428011417389,
0.01703772507607937,
-0.04915685951709747,
0.05537004396319389,
-0.022355953231453896,
0.08887437731027603,
-0.04420265927910805,
0.03183848410844803,
0.002909440081566572,
-0.030404621735215187,
-0.01214285846799612,
-0.013933511450886726,
-0.011175272054970264,
-0.0027821743860840797,
-0.012337207794189453,
0.0495435930788517,
0.06039620190858841,
-0.004002112429589033,
-0.0020920459646731615,
-0.007271486800163984,
0.01704495958983898,
0.020730067044496536,
-0.0012256031623110175,
0.0025714084040373564,
0.004197399131953716,
-0.07050302624702454,
0.010052734054625034,
0.03443221375346184,
0.04766117408871651,
-0.012219593860208988,
0.0027790043968707323,
-0.028313852846622467,
0.02635054849088192,
0.056325312703847885,
0.008530164137482643,
-0.008036778308451176,
0.010720917955040932,
-0.007859932258725166,
-0.061114583164453506,
-0.021704157814383507,
0.0007092946325428784,
-0.00010156755888601765,
-0.04571815952658653,
-0.011564969085156918,
-0.10503464937210083,
0.023518770933151245,
-0.02101357840001583,
-0.03044154681265354,
-0.022167637944221497,
-0.011750787496566772,
-0.057244058698415756,
0.022718368098139763,
0.05385369807481766,
0.026013849303126335,
-0.000027127050998387858,
0.06560882180929184,
0.06053874269127846,
0.017742495983839035,
-0.03804544359445572,
-0.0046258349902927876,
0.03480549529194832,
-0.013220162130892277,
0.05264700576663017,
0.03928472101688385,
0.03900277614593506,
0.028606904670596123,
-0.06751957535743713,
0.036280374974012375,
-0.013593810610473156,
0.0015738566871732473,
0.027310414239764214,
-0.004146299324929714,
0.0568925216794014,
-0.017138605937361717,
-0.012400676496326923,
-0.3311358690261841,
-0.03188231214880943,
0.025256721302866936,
-0.022175045683979988,
0.03079872578382492,
-0.09510712325572968,
0.057661090046167374,
-0.05721115320920944,
-0.053008221089839935,
-0.06944374740123749,
0.02770453691482544,
0.07108085602521896,
-0.04558328911662102,
-0.009977786801755428,
0.015469668433070183,
0.03593100607395172,
0.07711365073919296,
-0.025004632771015167,
-0.007844805717468262,
0.011096702888607979,
0.03642268478870392,
-0.04072124883532524,
0.022286057472229004,
0.027901802211999893,
0.045922476798295975,
-0.011422687210142612,
0.07184012979269028,
-0.019205689430236816,
0.04369833320379257,
-0.027756182476878166,
-0.015411426313221455,
0.06861020624637604,
-0.012217315845191479,
-0.08988821506500244,
0.028465205803513527,
-0.08905155211687088,
0.06948742270469666,
0.024764828383922577,
-0.0027581537142395973,
-0.03993179276585579,
-0.06145639345049858,
-0.015919385477900505,
-0.041781987994909286,
-0.030669044703245163,
-0.0729043036699295,
-0.01528090052306652,
0.01624106429517269,
-0.014976601116359234,
-0.040231797844171524,
-0.015026992186903954,
0.07255654782056808,
0.006022545509040356,
-0.02096993662416935,
-0.021320197731256485,
0.05248232185840607,
-0.03146904706954956,
-0.0649883821606636,
-0.026102788746356964,
-0.04413782060146332,
-0.025426778942346573,
-0.004415914416313171,
0.0066217887215316296,
0.030138816684484482,
-0.01118555199354887,
0.000998802832327783,
0.05240434408187866,
0.07659203559160233,
0.03741370141506195,
0.030421769246459007,
0.018210696056485176,
0.005352070089429617,
0.1201021820306778,
-0.0655919685959816,
-0.016542816534638405,
0.06105981767177582,
0.011202815920114517,
-0.021525200456380844,
-0.019517771899700165,
-0.04485635459423065,
0.005065346602350473,
-0.036403995007276535,
-0.03775057569146156,
0.035223063081502914,
0.011949620209634304,
-0.062340375036001205,
-0.003238879842683673,
0.005655426066368818,
0.006563542876392603,
0.03904658555984497,
-0.016826990991830826,
-0.0411096028983593,
0.01740863174200058,
-0.03059079311788082,
-0.03548375889658928,
0.07233114540576935,
0.0219496488571167,
-0.2723004221916199,
0.0062278746627271175,
0.03280506283044815,
-0.058215610682964325,
0.021071460098028183,
-0.012838110327720642,
0.002039262093603611,
-0.037294480949640274,
-0.05399312078952789,
0.03216055408120155,
-0.04824288189411163,
0.034250400960445404,
0.04809720441699028,
0.013796715997159481,
-0.017145300284028053,
0.020756546407938004,
0.053194042295217514,
-0.028643788769841194,
0.03312203660607338,
-0.0408075675368309,
-0.02276783064007759,
0.0015209157718345523,
0.13107222318649292,
0.07521641254425049,
-0.030459033325314522,
0.04599147289991379,
-0.014208606444299221,
0.022877009585499763,
0.014875543303787708,
0.04603332281112671,
-0.018667038530111313,
-0.005655090324580669,
0.07629218697547913,
-0.0179294440895319,
0.07559271156787872,
0.03422828018665314,
-0.06279361993074417,
-0.038219574838876724,
0.04455798491835594,
0.02258151024580002,
0.0018622797215357423,
0.02371567115187645,
0.018099891021847725,
-0.0249463003128767,
0.08463133126497269,
-0.08115996420383453,
-0.05370844155550003,
-0.04998831823468208,
0.0009049013024196029,
-0.025674989446997643,
-0.011654139496386051,
-0.03464864566922188,
0.032460909336805344,
0.059561021625995636,
0.015980646014213562,
-0.01199804525822401,
-0.04610578343272209,
-0.0051259854808449745,
-0.013756869360804558,
-0.03782281652092934,
-0.051892176270484924,
0.008371075615286827,
-0.06616203486919403,
0.07535183429718018,
0.016092199832201004
] |
PMID:27129 | Acid aspiration during laparoscopy. | The prophylactic efficacy of pre-operative antacid administration was shown by comparing the pH and volumes of gastric contents in 50 female patients treated with 10 ml of Mist. Magnesium Trisilicate B.P.C. with that of 50 controls. Routine antacid prophylaxis was advocated for all patients undergoing pelvic laparoscopy as intra-operative conditions increase the risk of pulmonary acid aspiration. | Acid aspiration during laparoscopy. The prophylactic efficacy of pre-operative antacid administration was shown by comparing the pH and volumes of gastric contents in 50 female patients treated with 10 ml of Mist. Magnesium Trisilicate B.P.C. with that of 50 controls. Routine antacid prophylaxis was advocated for all patients undergoing pelvic laparoscopy as intra-operative conditions increase the risk of pulmonary acid aspiration. | [
0.01759546808898449,
0.0020785382948815823,
-0.020450402051210403,
0.06958755105733871,
-0.03875670209527016,
0.0015781013062223792,
0.07027385383844376,
0.08590097725391388,
-0.05631265416741371,
-0.026065653190016747,
-0.003894141409546137,
-0.05372308939695358,
0.003849808592349291,
0.030059698969125748,
-0.04553767293691635,
-0.01952235959470272,
0.03358815982937813,
-0.03042670153081417,
-0.053070683032274246,
0.001977091422304511,
0.001103960326872766,
0.029630502685904503,
0.025988692417740822,
-0.025710701942443848,
0.012725340202450752,
0.06462381780147552,
0.010921148583292961,
-0.02177896909415722,
-0.06462728977203369,
-0.17333795130252838,
-0.00932607427239418,
-0.023777281865477562,
-0.03030778467655182,
-0.0204743854701519,
-0.009247037582099438,
0.012064984999597073,
0.0213992390781641,
0.10188847780227661,
-0.0164957158267498,
0.039935916662216187,
0.05485939979553223,
-0.028222311288118362,
-0.01678325980901718,
0.00566501822322607,
-0.03641555830836296,
-0.023213785141706467,
0.029384205117821693,
-0.03895273059606552,
0.11603597551584244,
0.07553257048130035,
0.020153889432549477,
0.0011267808731645346,
0.00007991917664185166,
0.05515921115875244,
-0.05709444358944893,
-0.092722587287426,
-0.05081315338611603,
-0.019731344655156136,
-0.04260091111063957,
0.03904150053858757,
-0.0021281372755765915,
0.013586458750069141,
-0.14726124703884125,
0.06746608763933182,
0.0036751837469637394,
-0.0074370927177369595,
-0.021177301183342934,
-0.07904528081417084,
0.0730484202504158,
0.08177393674850464,
-0.05959891155362129,
0.022904103621840477,
0.014329723082482815,
0.062172647565603256,
-0.023110918700695038,
0.010935114696621895,
0.058665402233600616,
-0.024006439372897148,
-0.04748758301138878,
0.02485419623553753,
-0.05585801228880882,
-0.0005700172041542828,
-0.04645936191082001,
-0.050438567996025085,
0.015886442735791206,
-0.08158369362354279,
0.024418773129582405,
0.038028065115213394,
-0.03210366889834404,
-0.017567848786711693,
0.016216516494750977,
-0.016306309029459953,
-0.04580173268914223,
0.013106206431984901,
0.004090696573257446,
-0.0026919401716440916,
0.005320628173649311,
0.022682467475533485,
0.00787313375622034,
0.3296026885509491,
0.00711268512532115,
0.017060711979866028,
-0.009127023629844189,
-0.03880811482667923,
-0.01750967465341091,
-0.034122467041015625,
0.035765472799539566,
-0.0010488685220479965,
0.01939220353960991,
0.06234379857778549,
0.010839760303497314,
0.05105931684374809,
0.026452962309122086,
-0.0329284742474556,
-0.024161238223314285,
0.10770484805107117,
0.10111678391695023,
-0.009418092668056488,
-0.011344410479068756,
-0.04508911818265915,
-0.042279377579689026,
-0.0228293277323246,
0.029466474428772926,
0.014887703582644463,
0.03816172108054161,
-0.005455576814711094,
-0.0032380789052695036,
0.08384972810745239,
-0.07440275698900223,
0.0348423533141613,
0.07208376377820969,
-0.024384193122386932,
0.029994135722517967,
0.02165200375020504,
0.04304150491952896,
-0.04954754561185837,
0.04997704178094864,
-0.014185531996190548,
0.003030485939234495,
0.006609527859836817,
-0.08307277411222458,
-0.05460376664996147,
0.01842324249446392,
-0.06012149900197983,
-0.0036024367436766624,
0.10816383361816406,
-0.05816002935171127,
-0.038695380091667175,
-0.01795308105647564,
-0.025782164186239243,
-0.0057421098463237286,
0.041413813829422,
0.009364406578242779,
0.09983418881893158,
-0.003538132645189762,
-0.017315112054347992,
0.0000047708931560919154,
0.06116507574915886,
-0.015919016674160957,
-0.020455703139305115,
-0.05034758895635605,
-0.06278274953365326,
-0.0511770099401474,
0.08657723665237427,
0.014055160805583,
-0.03188367187976837,
-0.04965728521347046,
0.006985837128013372,
-0.046885959804058075,
0.016028407961130142,
-0.01049108151346445,
-0.016354933381080627,
-0.04060859605669975,
-0.05754031985998154,
0.006830902304500341,
0.033281005918979645,
-0.017641887068748474,
0.0014813161687925458,
0.010522620752453804,
-0.005979183595627546,
0.04458889365196228,
0.0416383370757103,
-0.005500477738678455,
-0.035281065851449966,
-0.022188881412148476,
-0.018400870263576508,
-0.07597322762012482,
0.03545127809047699,
0.06432133913040161,
0.009648926556110382,
-0.007298900745809078,
0.0017922952538356185,
-0.03811352327466011,
0.009084724821150303,
0.04321986436843872,
-0.012742538936436176,
0.013500165194272995,
-0.04117671400308609,
-0.06376996636390686,
-0.0029009529389441013,
0.010409269481897354,
-0.000499873363878578,
-0.005962125491350889,
0.04978612810373306,
0.08194075524806976,
0.02281840518116951,
0.015569823794066906,
0.028443416580557823,
0.04268275946378708,
0.0029083378612995148,
-0.03194836899638176,
0.07607347518205643,
-0.030708832666277885,
0.006768052466213703,
-0.1077018454670906,
0.0725192055106163,
0.0062676165252923965,
-0.014230689965188503,
0.03406854346394539,
0.02208997868001461,
0.1067051962018013,
0.011087685823440552,
-0.011611245572566986,
-0.30459362268447876,
0.018910542130470276,
-0.014583762735128403,
-0.016947060823440552,
0.09535467624664307,
-0.05839529260993004,
0.010457287542521954,
0.04364556819200516,
-0.036166369915008545,
-0.08388885855674744,
0.01222500205039978,
0.058536823838949203,
-0.024949215352535248,
0.07365715503692627,
-0.03030138835310936,
0.08616624772548676,
-0.011299782432615757,
-0.02991100400686264,
0.06971560418605804,
0.027224387973546982,
0.004320908337831497,
-0.04851464554667473,
-0.01422776561230421,
0.012182009406387806,
0.017813527956604958,
-0.006763617508113384,
0.09646422415971756,
-0.06287520378828049,
-0.008217226713895798,
-0.05369849503040314,
0.0377017967402935,
-0.01848778687417507,
-0.015657322481274605,
-0.030862892046570778,
-0.029931511729955673,
-0.026618128642439842,
-0.011584804393351078,
0.00793187040835619,
-0.009470085613429546,
-0.001738348393701017,
-0.025557205080986023,
-0.004652806092053652,
-0.07782067358493805,
-0.0541822649538517,
-0.04869646579027176,
-0.04537931829690933,
-0.02020719274878502,
0.06715843826532364,
-0.03290129825472832,
-0.012976611964404583,
0.01953883469104767,
0.09173288941383362,
-0.027903353795409203,
-0.0066665224730968475,
-0.009295940399169922,
-0.044210296124219894,
0.017004791647195816,
0.007409817073494196,
-0.056030113250017166,
-0.02157466672360897,
0.0020398760680109262,
-0.02233610674738884,
0.054840460419654846,
0.00446655647829175,
-0.015551138669252396,
-0.020039072260260582,
0.019521154463291168,
-0.00974773708730936,
0.04982339218258858,
0.01393493264913559,
0.007426475640386343,
0.12235646694898605,
-0.1044435203075409,
-0.03984403610229492,
0.053691502660512924,
0.024715634062886238,
0.007402161601930857,
0.04017627239227295,
-0.07179953157901764,
-0.01575588621199131,
0.020884791389107704,
-0.0030321995727717876,
0.07217925786972046,
-0.02164417877793312,
-0.0166518185287714,
0.09512457996606827,
-0.05405198782682419,
0.027195289731025696,
-0.029664911329746246,
-0.027237646281719208,
-0.03373078629374504,
0.014880917966365814,
-0.064414843916893,
0.03882594406604767,
0.03621303290128708,
0.00751483766362071,
-0.25507813692092896,
-0.00472484203055501,
0.010625630617141724,
0.05032522976398468,
-0.027357233688235283,
-0.014324691146612167,
0.018717903643846512,
-0.03264521434903145,
-0.041207410395145416,
0.036302339285612106,
-0.017184628173708916,
-0.0020109026227146387,
0.042994219809770584,
0.002048567868769169,
-0.03633229061961174,
0.0218232162296772,
0.03392601013183594,
-0.016061291098594666,
0.01187028270214796,
-0.060700923204422,
-0.011007648892700672,
-0.08371168375015259,
0.11824339628219604,
0.006294405087828636,
-0.03427776321768761,
0.04823734238743782,
0.016766713932156563,
0.039974797517061234,
0.019997676834464073,
-0.012561315670609474,
0.0300866961479187,
-0.007104361895471811,
0.06527207046747208,
-0.016032934188842773,
0.038106102496385574,
-0.015155775472521782,
-0.06769304722547531,
0.04067225009202957,
0.04023197665810585,
0.05709113925695419,
-0.0356154665350914,
-0.022236118093132973,
0.008251510560512543,
-0.02154187485575676,
0.03335133194923401,
-0.06673946976661682,
0.03305749222636223,
-0.04828950762748718,
-0.026211455464363098,
0.004344988614320755,
0.002193253953009844,
-0.04312228783965111,
0.07138781249523163,
0.011417067609727383,
-0.014904587529599667,
0.08303871750831604,
0.035581447184085846,
0.004284390714019537,
-0.050259869545698166,
0.020085718482732773,
0.04507060721516609,
0.004199571907520294,
-0.02355918101966381,
0.054010916501283646,
0.05965811759233475
] |
PMID:27131 | Placental transfer of lidocaine: effects of fetal acidosis. | To investigate whether fetal acidosis increases the placental transfer of lidocaine, resulting in higher fetal blood levels of the drug, lidocaine was infused intravenously into ten pregnant ewes to maintain plasma levels of 2-4 microgram/ml. After maternal-fetal equilibrium was reached, the fetus was made acidotic by infusing lactic acid intravenously. Fetal blood pH decreased from 7.35 to 7.10. With fetal acidemia, fetal blood lidocaine levels increased significantly from 1.60 +/- 0.11 microgram/ml to 2.72 +/- 0.26 microgram/ml. The fetal-maternal lidocaine ratio increased from 0.76 to 1.21. Correction of the acidosis by bicarbonate infusion returned the fetal-maternal ratios to control values. It is concluded that acidosis in the fetus may result in trapping of ionized lidocaine in the fetal circulation and increase the transfer of lidocaine across the placenta. | Placental transfer of lidocaine: effects of fetal acidosis. To investigate whether fetal acidosis increases the placental transfer of lidocaine, resulting in higher fetal blood levels of the drug, lidocaine was infused intravenously into ten pregnant ewes to maintain plasma levels of 2-4 microgram/ml. After maternal-fetal equilibrium was reached, the fetus was made acidotic by infusing lactic acid intravenously. Fetal blood pH decreased from 7.35 to 7.10. With fetal acidemia, fetal blood lidocaine levels increased significantly from 1.60 +/- 0.11 microgram/ml to 2.72 +/- 0.26 microgram/ml. The fetal-maternal lidocaine ratio increased from 0.76 to 1.21. Correction of the acidosis by bicarbonate infusion returned the fetal-maternal ratios to control values. It is concluded that acidosis in the fetus may result in trapping of ionized lidocaine in the fetal circulation and increase the transfer of lidocaine across the placenta. | [
-0.014744891785085201,
-0.019028713926672935,
-0.018096627667546272,
0.05098744481801987,
-0.03269238397479057,
0.012561597861349583,
0.09930241107940674,
0.03217058628797531,
-0.04864402487874031,
0.009924395941197872,
-0.011069857515394688,
-0.006471866276115179,
-0.05078050121665001,
0.03081231191754341,
-0.0580238401889801,
-0.0023307641968131065,
-0.012541230767965317,
-0.00837769079953432,
-0.17384186387062073,
0.0067208921536803246,
0.021824365481734276,
0.03519648313522339,
0.00829372089356184,
-0.02281070686876774,
0.056772034615278244,
0.005959253292530775,
-0.005278473719954491,
-0.013473359867930412,
-0.09097931534051895,
-0.17301228642463684,
0.014064089395105839,
-0.03896124288439751,
-0.006089990027248859,
0.006312916520982981,
-0.05613059550523758,
0.040615107864141464,
-0.01829415000975132,
0.047899600118398666,
-0.031930599361658096,
0.06178456172347069,
0.08201854676008224,
0.03068423829972744,
-0.01868189126253128,
0.019513413310050964,
0.005043515469878912,
0.027844274416565895,
-0.004441805649548769,
0.00828635785728693,
0.06757862865924835,
0.015364286489784718,
0.015663785859942436,
-0.021060049533843994,
0.01986124739050865,
0.05458610877394676,
-0.06946147978305817,
-0.02613009512424469,
0.029504651203751564,
0.019702745601534843,
0.04152648523449898,
0.054756391793489456,
0.02306714467704296,
0.10136768966913223,
-0.13343928754329681,
0.06212267279624939,
0.03974233567714691,
-0.03458350896835327,
0.0016939538763836026,
-0.05221386253833771,
0.024280764162540436,
0.09336905181407928,
-0.07724887877702713,
0.03485463187098503,
0.054272860288619995,
0.024538997560739517,
0.023556509986519814,
-0.03985113650560379,
0.036236703395843506,
0.01833347976207733,
0.013974430039525032,
-0.0744256004691124,
-0.07185601443052292,
-0.0672873854637146,
-0.02423930913209915,
-0.015058167278766632,
-0.005907534155994654,
-0.04253075271844864,
0.038767799735069275,
-0.003508856752887368,
-0.0400397963821888,
0.02147090807557106,
-0.012948007322847843,
-0.11309734731912613,
-0.04747745394706726,
-0.023785626515746117,
-0.07219240069389343,
0.008772177621722221,
0.006508165504783392,
-0.02714179828763008,
-0.043552152812480927,
0.34907266497612,
-0.03717994689941406,
0.0060415505431592464,
-0.040127214044332504,
0.022447722032666206,
-0.015140242874622345,
0.0285708699375391,
0.017217911779880524,
0.05049096792936325,
-0.031516317278146744,
-0.005172298289835453,
0.029354961588978767,
0.05472183972597122,
0.023028148338198662,
0.009057373739778996,
-0.09429749101400375,
0.028467953205108643,
0.06091926246881485,
0.032011087983846664,
0.05138350650668144,
0.04925401881337166,
-0.020100390538573265,
-0.02349970117211342,
0.02494306117296219,
-0.0714343935251236,
0.05660071596503258,
0.04186193272471428,
-0.0035914022009819746,
0.10184895247220993,
-0.006011671386659145,
-0.019214574247598648,
0.0360775962471962,
0.010889620520174503,
-0.04152491316199303,
0.0515616275370121,
-0.00807584822177887,
-0.03758764639496803,
-0.011622068472206593,
-0.032699521631002426,
0.015055880881845951,
0.014048540033400059,
-0.013880196027457714,
-0.04674693942070007,
-0.05310415104031563,
-0.07082010805606842,
-0.05753007531166077,
0.11187029629945755,
0.013032987713813782,
-0.013311302289366722,
-0.003989384975284338,
0.01754571497440338,
-0.0421573705971241,
0.05400862917304039,
-0.027295075356960297,
0.01379362028092146,
0.009705101139843464,
0.007116074673831463,
-0.036203451454639435,
0.07432664930820465,
0.015726473182439804,
-0.04006347805261612,
0.034631453454494476,
-0.0697302520275116,
-0.05530756339430809,
0.06024956703186035,
-0.015765387564897537,
0.017538901418447495,
-0.0687185749411583,
-0.025699863210320473,
-0.00910442415624857,
0.02551424317061901,
0.02752317115664482,
-0.026178115978837013,
0.011797895655035973,
-0.06265034526586533,
0.0038197541143745184,
-0.021373525261878967,
-0.02315865270793438,
0.009395907633006573,
0.04838012158870697,
-0.051669348031282425,
0.06354942172765732,
-0.007101239170879126,
0.03664007782936096,
0.029128799214959145,
0.050814758986234665,
-0.04591463506221771,
-0.051186736673116684,
-0.011662948876619339,
0.02854694239795208,
0.02177240140736103,
0.0019703239668160677,
0.008297638967633247,
-0.041252899914979935,
0.017284736037254333,
0.036221131682395935,
-0.013453363440930843,
-0.012814605608582497,
0.01552529726177454,
0.002759138122200966,
-0.015643425285816193,
0.09211109578609467,
-0.006367430556565523,
-0.06555966287851334,
0.09923368692398071,
0.08644650131464005,
0.04012591391801834,
-0.0051828669384121895,
-0.005343248136341572,
0.03514081612229347,
0.00047229594201780856,
-0.011812493205070496,
0.08435937017202377,
-0.00011406242992961779,
0.0013663432328030467,
-0.0777045413851738,
0.05822776257991791,
0.013744432479143143,
0.020588921383023262,
0.022100968286395073,
0.0010400504106655717,
0.06050831824541092,
-0.09361044317483902,
0.02342144586145878,
-0.3093215823173523,
0.026306524872779846,
0.031869497150182724,
-0.04940017685294151,
0.011057181283831596,
-0.040467239916324615,
0.04001978039741516,
-0.018575672060251236,
-0.011633733287453651,
-0.027890147641301155,
0.03294443339109421,
0.055721916258335114,
-0.052913881838321686,
0.01976141892373562,
0.012218687683343887,
-0.0345265232026577,
0.018074046820402145,
-0.057724788784980774,
0.0671839490532875,
0.063798688352108,
0.006512180436402559,
0.008965672925114632,
-0.0064369793981313705,
0.07765895873308182,
0.08757150173187256,
-0.002571357414126396,
0.08139356970787048,
-0.04855450987815857,
0.061432138085365295,
-0.04954862222075462,
-0.021706217899918556,
0.028210023418068886,
-0.03733494132757187,
-0.04388868808746338,
-0.006525783333927393,
0.04868984594941139,
0.030525770038366318,
0.014310787431895733,
-0.006682067643851042,
-0.0006913651595823467,
-0.053933512419462204,
0.006645143032073975,
-0.009945825673639774,
-0.04754467308521271,
-0.03220931440591812,
0.041667334735393524,
-0.02993890456855297,
-0.05313042551279068,
-0.003614524146541953,
-0.04344848915934563,
0.05061621963977814,
0.0037508816458284855,
-0.008401047438383102,
-0.04567798227071762,
0.04301806911826134,
-0.05389383062720299,
-0.023168811574578285,
0.028359564021229744,
-0.07766401022672653,
-0.015095662325620651,
0.006481972057372332,
0.0012304579140618443,
-0.0005425852723419666,
-0.0500580258667469,
-0.01244356483221054,
0.041542068123817444,
0.007087841629981995,
0.014038421213626862,
0.041404854506254196,
0.009936464950442314,
0.02251475676894188,
0.12667150795459747,
-0.052096255123615265,
0.008119898848235607,
0.07029344141483307,
-0.043401088565588,
-0.06070992723107338,
-0.009396097622811794,
-0.082570381462574,
0.007554980926215649,
-0.01190088503062725,
-0.05138476565480232,
0.005874659400433302,
-0.0006505181081593037,
-0.0014572974760085344,
0.03854162618517876,
0.042102184146642685,
0.017795827239751816,
-0.04224621504545212,
-0.011888156644999981,
0.018436061218380928,
0.008309023454785347,
-0.07625085115432739,
-0.008156257681548595,
0.01875855028629303,
0.03776787966489792,
-0.24587687849998474,
-0.040280360728502274,
0.019399119541049004,
-0.020978493615984917,
-0.006937385071069002,
-0.04898853972554207,
0.02367217279970646,
-0.013988927006721497,
0.014794954098761082,
-0.0028830990195274353,
-0.025344833731651306,
0.0003227127599529922,
0.03228592500090599,
0.08888436108827591,
0.024425240233540535,
0.023824555799365044,
-0.0020757548045367002,
-0.011515526100993156,
-0.07229439914226532,
-0.0017716495785862207,
0.005177121609449387,
-0.039045147597789764,
0.15725742280483246,
0.036774393171072006,
-0.04621312394738197,
0.004558675456792116,
0.003635452827438712,
0.04621491953730583,
0.0028028632514178753,
-0.004538706038147211,
-0.022271161898970604,
0.06781948357820511,
-0.017962738871574402,
0.013418853282928467,
0.007049018982797861,
0.0014848816208541393,
-0.047825440764427185,
-0.018095165491104126,
0.022949889302253723,
0.014761069789528847,
0.032476186752319336,
0.0014413257595151663,
-0.006310626864433289,
-0.061895307153463364,
0.07689571380615234,
-0.033328548073768616,
-0.027861665934324265,
-0.04750295355916023,
0.022260578349232674,
0.038091085851192474,
0.0030492525547742844,
-0.014911261387169361,
0.04555138573050499,
-0.024863572791218758,
0.017338760197162628,
0.00125663704238832,
0.007017574273049831,
-0.03511829301714897,
-0.019037969410419464,
0.03708374500274658,
-0.007573586888611317,
-0.0451115258038044,
0.015088967978954315,
0.02734449878334999,
0.0759674459695816
] |
PMID:27132 | Hemoglobin Creteil: oxygen transport by erythrocytes. In-vitro and in-vivo studies in a high oxygen-affinity mutant hemoglobin. | Hemoglobin Hb) Creteil alpha2beta(2)89 (F5) Ser leads to Asn is a high oxygen-affinity variant that has a low cooperativity, a decreased Bohr effect, and does not interact with diphosphoglycerate (DPG) (1). This hemoglobin variant was silent by routine electrophoresis. Careful analyses of the oxygen dissociation curves of erythrocytes, determined at varying pH and Pco2 in fresh and DPG-depleted cells, gave extensive information on the abnormal function of the mutant hemoglobin. From the O2 dissociation curves of the erythrocytes of the heterozygous subject it appeared that Hb Creteil is inappropriate for O2 transport to the tissues because it remained completely saturated with O2 at normal physiologic levels of Po2. In-vivo measurements showed that only one half of the total hemoglobin present actually participated in oxygen transport. Polycythemia should therefore be maintained within clinically tolerable limits because it helps to keep arterial Po2 and Pvo2 close to their normal values and thus protects the individual from a permanent increase in blood flow. | Hemoglobin Creteil: oxygen transport by erythrocytes. In-vitro and in-vivo studies in a high oxygen-affinity mutant hemoglobin. Hemoglobin Hb) Creteil alpha2beta(2)89 (F5) Ser leads to Asn is a high oxygen-affinity variant that has a low cooperativity, a decreased Bohr effect, and does not interact with diphosphoglycerate (DPG) (1). This hemoglobin variant was silent by routine electrophoresis. Careful analyses of the oxygen dissociation curves of erythrocytes, determined at varying pH and Pco2 in fresh and DPG-depleted cells, gave extensive information on the abnormal function of the mutant hemoglobin. From the O2 dissociation curves of the erythrocytes of the heterozygous subject it appeared that Hb Creteil is inappropriate for O2 transport to the tissues because it remained completely saturated with O2 at normal physiologic levels of Po2. In-vivo measurements showed that only one half of the total hemoglobin present actually participated in oxygen transport. Polycythemia should therefore be maintained within clinically tolerable limits because it helps to keep arterial Po2 and Pvo2 close to their normal values and thus protects the individual from a permanent increase in blood flow. | [
-0.05814338102936745,
0.055338211357593536,
-0.039026882499456406,
0.0018719324143603444,
-0.04620395600795746,
-0.021815072745084763,
0.08826448023319244,
0.035578928887844086,
-0.036569371819496155,
-0.046686094254255295,
0.02082710713148117,
-0.1258038431406021,
0.008899991400539875,
0.020499637350440025,
-0.039140988141298294,
0.0015475591644644737,
0.012321311980485916,
-0.04110555350780487,
-0.06272796541452408,
0.046866510063409805,
-0.016426455229520798,
-0.04881281778216362,
0.03877314180135727,
-0.052831705659627914,
0.067392997443676,
0.07380975782871246,
-0.01783384196460247,
0.037119731307029724,
-0.0661928802728653,
-0.18847547471523285,
-0.006961184553802013,
-0.03278947249054909,
-0.046509236097335815,
0.01746284030377865,
-0.07661446928977966,
-0.016460467129945755,
-0.05122785270214081,
0.0007700073183514178,
-0.07490695267915726,
0.06894635409116745,
0.04439784586429596,
-0.006931780371814966,
-0.020445115864276886,
0.03318144381046295,
0.02694736048579216,
0.02941306307911873,
-0.01376904733479023,
0.0032223109155893326,
0.05254665017127991,
0.02041972242295742,
0.016031455248594284,
0.03341924771666527,
-0.0006833303486928344,
0.010545538738369942,
0.0010784482583403587,
-0.01639116182923317,
-0.010109388269484043,
-0.021468395367264748,
0.007449412252753973,
0.026409318670630455,
0.02623761259019375,
0.03768497332930565,
-0.12615966796875,
0.03778655827045441,
0.11980558186769485,
-0.002595815807580948,
-0.01370417419821024,
-0.08545592427253723,
0.016220826655626297,
-0.02338254265487194,
-0.0235338993370533,
0.0186212956905365,
0.004806805402040482,
0.029976477846503258,
-0.0284019336104393,
0.016915416345000267,
-0.00011702883784892038,
-0.04533667862415314,
0.020357606932520866,
0.006241572089493275,
-0.029149813577532768,
-0.041807979345321655,
-0.04002450779080391,
-0.02873077429831028,
0.039644379168748856,
0.013193882070481777,
-0.01045721210539341,
-0.12433475255966187,
-0.06898960471153259,
0.0018373706843703985,
0.0004715416580438614,
-0.054341692477464676,
0.001861973782069981,
0.04000825434923172,
-0.07172289490699768,
0.030642841011285782,
0.009102690033614635,
-0.027225522324442863,
-0.0019007805967703462,
0.37654173374176025,
0.01003331784158945,
0.0402701199054718,
0.00017851214215625077,
0.04313857480883598,
0.028855856508016586,
-0.08018111437559128,
0.007779350038617849,
0.03343244269490242,
0.03004354052245617,
0.026019548997282982,
-0.004994352348148823,
-0.015615670941770077,
0.06832657009363174,
-0.022990262135863304,
-0.020020872354507446,
0.027689119800925255,
0.02079295553267002,
0.010621761903166771,
-0.06654881685972214,
0.05652979388833046,
-0.022228842601180077,
-0.027044180780649185,
0.055794719606637955,
0.054210059344768524,
0.0527152344584465,
0.04448607191443443,
-0.01657724939286709,
0.07606896013021469,
0.045144110918045044,
0.01773499697446823,
0.06787677854299545,
0.04771586135029793,
-0.06541649252176285,
-0.055222924798727036,
-0.004848557524383068,
0.024735145270824432,
0.017261354252696037,
-0.0200028233230114,
-0.021381964907050133,
-0.025767093524336815,
-0.008217078633606434,
-0.01358486246317625,
0.004248428624123335,
-0.03885433077812195,
-0.048975493758916855,
0.0645504742860794,
0.0549517422914505,
0.018949169665575027,
-0.0146471643820405,
-0.030659269541502,
-0.03347652032971382,
0.028155818581581116,
-0.03281562775373459,
-0.0026365623343735933,
-0.07200243324041367,
0.07656121999025345,
-0.0307813361287117,
0.04050415754318237,
0.02442413568496704,
-0.07799241691827774,
-0.07149629294872284,
-0.018605077639222145,
-0.0539003387093544,
0.02464698813855648,
-0.016032757237553596,
-0.002496513072401285,
-0.013879095204174519,
0.0036327966954559088,
0.017911989241838455,
0.034405000507831573,
0.004579148720949888,
-0.034262798726558685,
0.0275314599275589,
-0.04352583736181259,
0.03912568837404251,
-0.021577486768364906,
-0.023745274171233177,
-0.02939329855144024,
-0.015596000477671623,
-0.01205958891659975,
0.0147505272179842,
0.0020357922185212374,
-0.004304214380681515,
-0.03368370607495308,
0.04630494490265846,
0.007507049012929201,
-0.005293646827340126,
0.0636308342218399,
0.01756715402007103,
0.08667317777872086,
-0.0566219799220562,
0.007224025204777718,
-0.030339784920215607,
0.032080624252557755,
0.03494017943739891,
-0.01539359986782074,
0.01152154803276062,
0.013863664120435715,
-0.030383804813027382,
-0.0010866184020414948,
0.13075947761535645,
0.08166549354791641,
0.016882814466953278,
0.005672363098710775,
0.028753366321325302,
0.035811860114336014,
0.014806697145104408,
-0.039605364203453064,
-0.030733095481991768,
-0.022630600258708,
-0.024971241131424904,
-0.0026434031315147877,
-0.056398551911115646,
0.057056766003370285,
-0.06997755914926529,
-0.030505193397402763,
0.004892186261713505,
-0.030208827927708626,
0.02482251077890396,
-0.0003310531028546393,
0.07814910262823105,
-0.079445481300354,
0.02200618013739586,
-0.33392801880836487,
-0.0026149521581828594,
0.03970138728618622,
-0.011195282451808453,
0.0030433896463364363,
0.035041674971580505,
0.02805694192647934,
-0.018633440136909485,
-0.003521628910675645,
-0.05005273595452309,
0.04673255607485771,
0.05957745760679245,
-0.05958553031086922,
-0.030624425038695335,
0.0036096503026783466,
0.010386462323367596,
0.006755611393600702,
-0.025679228827357292,
-0.004385238979011774,
0.04122502729296684,
0.06034906581044197,
0.03143346309661865,
0.025661248713731766,
0.034472085535526276,
0.019564630463719368,
-0.01423231977969408,
0.10931509733200073,
-0.026113690808415413,
0.0408865250647068,
-0.07984792441129684,
0.021370453760027885,
0.00946792121976614,
0.0242354366928339,
-0.042415421456098557,
-0.010460707359015942,
-0.034573283046483994,
0.00843734946101904,
-0.022597212344408035,
0.03528769314289093,
-0.008514207787811756,
-0.004698605742305517,
0.003365784417837858,
-0.054508015513420105,
-0.04229684919118881,
-0.02679750882089138,
0.02907627820968628,
-0.0048743560910224915,
-0.01368285808712244,
0.000610645511187613,
-0.019904669374227524,
0.0219256654381752,
-0.019891485571861267,
0.03216717764735222,
0.0069427345879375935,
0.06630618870258331,
0.02437850832939148,
-0.004934719298034906,
0.04508396238088608,
-0.07968176156282425,
-0.020536884665489197,
-0.005208979360759258,
0.033177364617586136,
0.057810261845588684,
0.015181905589997768,
0.01266795489937067,
0.05697116628289223,
0.04262637346982956,
-0.03993351757526398,
0.09907062351703644,
0.021210867911577225,
0.029250573366880417,
0.08217115700244904,
-0.023246731609106064,
0.07641663402318954,
-0.03274928405880928,
0.07802573591470718,
-0.040078822523355484,
0.010303996503353119,
-0.028123071417212486,
-0.02490353211760521,
0.062170058488845825,
-0.010560297407209873,
0.03457975760102272,
0.01741774193942547,
0.004270697943866253,
0.031245680525898933,
0.038897376507520676,
0.009306282736361027,
-0.007017855532467365,
-0.0041695996187627316,
-0.02884499356150627,
-0.05840688571333885,
0.026993170380592346,
-0.089842289686203,
0.005698719993233681,
0.004114956129342318,
-0.26105761528015137,
-0.0386807881295681,
0.010663679800927639,
0.05578222498297691,
-0.011752795428037643,
-0.005538571625947952,
0.01842338591814041,
-0.06092444434762001,
-0.05777358636260033,
0.002739977091550827,
0.04861250892281532,
-0.029577380046248436,
0.050292328000068665,
0.00898177083581686,
0.023124124854803085,
0.02234046906232834,
0.03940640389919281,
-0.015041213482618332,
0.010836625471711159,
-0.04544694349169731,
-0.027040457352995872,
-0.05759630724787712,
0.13084347546100616,
-0.039465922862291336,
-0.041977282613515854,
-0.021985745057463646,
0.009831775911152363,
0.02623867429792881,
-0.05704840272665024,
-0.021121103316545486,
0.05345067009329796,
0.008128179237246513,
0.05140727013349533,
-0.05195802450180054,
0.01940874196588993,
0.037899959832429886,
-0.020421897992491722,
-0.02516159974038601,
0.0014177848352119327,
0.0012428117915987968,
0.019467080011963844,
-0.016970882192254066,
-0.04911619424819946,
-0.013621394522488117,
0.0940314456820488,
-0.003224084386602044,
-0.050312068313360214,
0.019251389428973198,
0.04346589744091034,
-0.04263581708073616,
-0.009078140370547771,
-0.03181212767958641,
0.0795646458864212,
-0.005153917707502842,
0.06575868278741837,
0.023140767589211464,
0.01899747923016548,
-0.04403255134820938,
-0.03093385323882103,
-0.010217406786978245,
0.025601347908377647,
-0.011588226072490215,
-0.05878966674208641,
0.06363200396299362,
0.0039405617862939835
] |
PMID:27134 | Cold injuries in Kashmir, December 1971. | A total of 847 cases of cold injury occurred within the short space of 2 weeks during the Indo-Pakistan conflict in Kashmir in December 1971. The management of these cases and their end results are described. A combination of drugs consisting of low-molecular-weight dextran, an anti-inflammatory agent, and a vasodilator was tried with encouraging results. A conservative attitude towards ablation of necrosed tissues paid good dividends. | Cold injuries in Kashmir, December 1971. A total of 847 cases of cold injury occurred within the short space of 2 weeks during the Indo-Pakistan conflict in Kashmir in December 1971. The management of these cases and their end results are described. A combination of drugs consisting of low-molecular-weight dextran, an anti-inflammatory agent, and a vasodilator was tried with encouraging results. A conservative attitude towards ablation of necrosed tissues paid good dividends. | [
-0.05364018678665161,
-0.0031366776674985886,
0.036565713584423065,
0.007406354416161776,
0.03171892464160919,
0.003056021872907877,
0.07443508505821228,
0.042026061564683914,
-0.08001940697431564,
-0.007479067891836166,
0.0374760702252388,
-0.008403124287724495,
0.059769876301288605,
0.02348974719643593,
-0.03434375301003456,
-0.009228317067027092,
0.009768879972398281,
-0.019747653976082802,
-0.03668395057320595,
0.021465763449668884,
-0.02684832736849785,
0.007493667304515839,
0.038204021751880646,
-0.026158219203352928,
0.010393979959189892,
0.08605309575796127,
0.021083882078528404,
-0.0022962293587625027,
-0.061665959656238556,
-0.1482895463705063,
-0.022556722164154053,
-0.05521806329488754,
0.02268923446536064,
-0.0334538072347641,
-0.05542460083961487,
0.032463423907756805,
-0.03367020934820175,
0.056968387216329575,
-0.019638828933238983,
0.007691633887588978,
0.04120206460356712,
0.08203226327896118,
0.013438484631478786,
-0.0049020214937627316,
-0.009227965027093887,
-0.021353377029299736,
-0.03288860246539116,
0.009590862318873405,
0.11192243546247482,
-0.022296832874417305,
0.013623043894767761,
0.0020414942409843206,
0.04715082421898842,
0.035149868577718735,
0.03189299628138542,
-0.06347091495990753,
-0.008648674935102463,
0.025904374197125435,
0.05092558637261391,
0.07162639498710632,
-0.020236453041434288,
0.07842037826776505,
-0.16990916430950165,
-0.004400530830025673,
-0.005666722543537617,
-0.019390812143683434,
-0.014570659026503563,
-0.03329889848828316,
0.03177937865257263,
0.033819012343883514,
0.039296772330999374,
0.09905080497264862,
0.04683544114232063,
-0.00175360101275146,
-0.06461793929338455,
-0.03429504483938217,
0.019809452816843987,
0.015546422451734543,
0.01463249884545803,
-0.00826106034219265,
-0.029563894495368004,
0.00833560898900032,
0.06066369265317917,
-0.050121504813432693,
-0.0034612782765179873,
-0.06435184925794601,
0.024730080738663673,
-0.0631655603647232,
-0.053192440420389175,
0.002907238667830825,
-0.0024465674068778753,
0.08835642784833908,
-0.01182533334940672,
0.015891961753368378,
-0.031814444810152054,
-0.013862032443284988,
-0.030842069536447525,
0.019017888233065605,
-0.02017846703529358,
0.36492234468460083,
0.021830106154084206,
-0.03656869754195213,
-0.03330360725522041,
0.029064128175377846,
-0.03715104982256889,
0.004911201074719429,
-0.01985536888241768,
-0.08955646306276321,
-0.013002418912947178,
0.020089982077479362,
0.06145434081554413,
0.004061987157911062,
0.034409698098897934,
0.005119894631206989,
0.012325460091233253,
0.00046796398237347603,
0.06267179548740387,
0.06167155131697655,
-0.0427737794816494,
0.038969092071056366,
-0.023709919303655624,
-0.05500048026442528,
0.022619852796196938,
-0.007422059308737516,
0.027417665347456932,
0.015072870999574661,
0.009321358054876328,
0.11346026510000229,
0.03225981071591377,
-0.03322945907711983,
0.07006166875362396,
-0.020528623834252357,
-0.014627121388912201,
-0.07022226601839066,
0.012950067408382893,
-0.011877058073878288,
0.00662159314379096,
-0.05490436777472496,
0.024467265233397484,
-0.0557706318795681,
0.001675873645581305,
-0.10221368074417114,
-0.04475414752960205,
-0.07089689373970032,
-0.07770223915576935,
0.06232646480202675,
-0.05963815376162529,
0.04122528061270714,
-0.07960564643144608,
-0.012027070857584476,
-0.02256922423839569,
-0.0011993495281785727,
-0.028222009539604187,
0.01280217245221138,
0.07914984226226807,
-0.028443582355976105,
0.040154095739126205,
-0.04038434103131294,
0.006426477804780006,
0.023454632610082626,
0.01525228563696146,
-0.07586313784122467,
0.0059417481534183025,
0.0720733180642128,
-0.015474723652005196,
-0.04573305696249008,
-0.00024327974824700505,
0.0039228154346346855,
0.003458224004134536,
0.04055136442184448,
-0.0056347777135670185,
-0.007098192349076271,
0.047258492559194565,
-0.00778049835935235,
0.00041803173371590674,
0.01661878637969494,
0.044557660818099976,
-0.06118603050708771,
0.022116346284747124,
0.02046499028801918,
0.014903426170349121,
-0.0151963010430336,
0.04931143671274185,
0.04954060912132263,
0.022286411374807358,
0.012854008004069328,
-0.018563421443104744,
0.01462558750063181,
0.04000415652990341,
0.012227880768477917,
-0.0458909310400486,
-0.021471725776791573,
-0.019225748255848885,
0.016596801578998566,
0.04261099174618721,
0.0018440964631736279,
-0.04916469380259514,
-0.005251594353467226,
0.04342231899499893,
0.04661600664258003,
0.02919151820242405,
-0.04111289232969284,
0.01171564869582653,
-0.020281707867980003,
-0.02843059040606022,
0.02029850333929062,
0.0017487247241660953,
-0.0394248440861702,
0.002071490278467536,
0.018165266141295433,
-0.019152430817484856,
0.08598363399505615,
0.016091186553239822,
-0.04276721924543381,
-0.02206893265247345,
-0.005937614012509584,
-0.025152722373604774,
0.052222199738025665,
0.0064621870405972,
-0.008221874013543129,
-0.005370791535824537,
-0.06203225627541542,
0.00030637599411420524,
-0.3122507333755493,
-0.013366874307394028,
0.02584855817258358,
-0.035354338586330414,
0.07305961102247238,
0.011227895505726337,
0.06152318790555,
-0.08955735713243484,
-0.02394499070942402,
0.01811082474887371,
0.09487272799015045,
0.06618326157331467,
-0.08100420236587524,
-0.016205092892050743,
-0.028138242661952972,
-0.010259168222546577,
-0.03478728234767914,
0.001963840564712882,
-0.0205449890345335,
0.04131046682596207,
0.07485045492649078,
-0.0058507598005235195,
-0.00616195285692811,
0.03453512489795685,
0.013153388164937496,
0.02186466194689274,
0.14944866299629211,
-0.02945552207529545,
-0.05876176059246063,
-0.061230458319187164,
-0.002967942738905549,
0.008703703060746193,
-0.017385665327310562,
-0.1094820648431778,
0.0005980855203233659,
-0.02847069315612316,
-0.037864185869693756,
-0.022694319486618042,
-0.02243383787572384,
0.04913460463285446,
-0.022853195667266846,
0.0077101727947592735,
-0.0036121553275734186,
0.03334810584783554,
0.012848930433392525,
-0.012338556349277496,
-0.02523123472929001,
-0.03579355403780937,
0.03673072159290314,
0.022072920575737953,
-0.013098631054162979,
-0.009242444299161434,
-0.03069513663649559,
0.015962833538651466,
-0.02952205017209053,
-0.023589013144373894,
-0.04247081279754639,
0.020340727642178535,
-0.04896518215537071,
0.004785091150552034,
0.01181322056800127,
-0.02385309711098671,
0.04215579852461815,
-0.00011110046034445986,
0.019492613151669502,
0.01314769871532917,
0.047920115292072296,
0.022669624537229538,
-0.01984836347401142,
0.029163068160414696,
-0.048947758972644806,
0.10243630409240723,
-0.03252062201499939,
-0.05476761609315872,
0.05075700581073761,
0.02738427370786667,
-0.0008425289415754378,
-0.010170630179345608,
-0.05381683632731438,
0.036815058439970016,
0.09085756540298462,
-0.037076935172080994,
0.002314083045348525,
0.02491082064807415,
0.00504948990419507,
-0.00199171993881464,
0.001137198880314827,
-0.016538895666599274,
0.01710466481745243,
-0.06534428894519806,
0.011916288174688816,
-0.00935306865721941,
-0.02080244943499565,
0.0254123006016016,
0.02833440527319908,
-0.006733683869242668,
-0.27961260080337524,
0.0689317137002945,
-0.021026188507676125,
-0.049453072249889374,
0.008215543814003468,
-0.027386851608753204,
-0.03597292676568031,
0.028623348101973534,
-0.0035586808808147907,
0.03047623299062252,
0.029626892879605293,
-0.023966416716575623,
0.06368955224752426,
-0.0036327310372143984,
0.005858194548636675,
-0.06298387795686722,
0.03195875138044357,
0.023242896422743797,
-0.007236603647470474,
0.0030375909991562366,
-0.0195830836892128,
-0.04519226402044296,
0.12193401902914047,
-0.02410951815545559,
-0.049245938658714294,
0.04543067514896393,
-0.012541279196739197,
0.046440862119197845,
-0.04058903083205223,
0.006204470992088318,
0.0385444350540638,
-0.0020642380695790052,
0.022058427333831787,
0.0031347551848739386,
-0.005009083542972803,
0.02101861871778965,
0.03737398609519005,
0.05951197072863579,
-0.0003291721222922206,
0.07362603396177292,
-0.04433795437216759,
-0.0025633592158555984,
0.04503791406750679,
0.04839958995580673,
0.08544538170099258,
0.008810321800410748,
0.0006656308542005718,
-0.16276448965072632,
-0.025766104459762573,
0.003348040394484997,
-0.04606819897890091,
0.015090616419911385,
0.04484026879072189,
0.030587762594223022,
0.04860682040452957,
0.015414661727845669,
0.02127874828875065,
-0.02014773339033127,
-0.05152410268783569,
-0.08863772451877594,
-0.046180400997400284,
0.01346171647310257,
-0.06549869477748871,
0.013704081997275352,
0.05318831279873848
] |
PMID:27135 | A disorder of biogenic amines in dihydropteridine reductase deficiency. | A severe deficiency of dihydropteridine reductase (DHPR) in liver, brain, and cultured skin fibroblasts was demonstrated in a child with hyperphenylalaninemia and an atypical form of phenylketonuria. DHPR is required for regeneration of the cofactor, tetrahydrobiopterin. The cofactor is essential in hydroxylation of aromatic amino acid precursors in the biosynthesis of neurotransmitters, serotonin, dopamine, and norepinephrine. In gray tissue at brain biopsy, dopamine was low at 3 ng per gram of tissue, serotonin was barely detected, and norepinephrine appeared high at 1600 ng per gram. In cerebrospinal fluid, homovanillic acid (HVA) was low normal at 33 ng/ml, 5-hydroxyindoleacetic acid (5-HIAA) was low at 4.2 ng/ml, and after a high dose of oral probenecid there was impaired accumulation of HVA to 128 ng/ml and 5-HIAA to 22.4 ng/ml. When the patient was 22 months of age, treatment with hydroxylated aromatic amino acid precursors was initiated, and after three months HVA and 5-HIAA levels were increased in CSF. The apparent restoration of biogenic amines in brain appears to have delayed the rate of neurological deterioration. DHPR activity in cultured skin fibroblasts of children with persistent hyperphenylalaninemia should permit early diagnosis and early treatment of this disorder. | A disorder of biogenic amines in dihydropteridine reductase deficiency. A severe deficiency of dihydropteridine reductase (DHPR) in liver, brain, and cultured skin fibroblasts was demonstrated in a child with hyperphenylalaninemia and an atypical form of phenylketonuria. DHPR is required for regeneration of the cofactor, tetrahydrobiopterin. The cofactor is essential in hydroxylation of aromatic amino acid precursors in the biosynthesis of neurotransmitters, serotonin, dopamine, and norepinephrine. In gray tissue at brain biopsy, dopamine was low at 3 ng per gram of tissue, serotonin was barely detected, and norepinephrine appeared high at 1600 ng per gram. In cerebrospinal fluid, homovanillic acid (HVA) was low normal at 33 ng/ml, 5-hydroxyindoleacetic acid (5-HIAA) was low at 4.2 ng/ml, and after a high dose of oral probenecid there was impaired accumulation of HVA to 128 ng/ml and 5-HIAA to 22.4 ng/ml. When the patient was 22 months of age, treatment with hydroxylated aromatic amino acid precursors was initiated, and after three months HVA and 5-HIAA levels were increased in CSF. The apparent restoration of biogenic amines in brain appears to have delayed the rate of neurological deterioration. DHPR activity in cultured skin fibroblasts of children with persistent hyperphenylalaninemia should permit early diagnosis and early treatment of this disorder. | [
-0.05330763757228851,
0.03316042199730873,
-0.04625362530350685,
0.028781183063983917,
-0.031717900186777115,
-0.01866786554455757,
0.02299393340945244,
0.05053084343671799,
-0.06776008754968643,
-0.04066568613052368,
0.051223814487457275,
-0.07918154448270798,
0.006798355374485254,
0.020968711003661156,
-0.02068677544593811,
0.027889061719179153,
0.01830933429300785,
-0.0029718291480094194,
-0.03898942470550537,
-0.004705772269517183,
-0.01922249048948288,
0.010216482914984226,
0.04706219583749771,
-0.01897752471268177,
0.02125198394060135,
-0.03383578732609749,
-0.017090706154704094,
-0.005148701369762421,
-0.03040541522204876,
-0.16294637322425842,
0.07922784984111786,
-0.009999837726354599,
0.03881081938743591,
-0.035005033016204834,
0.022158155217766762,
0.04067869856953621,
-0.03406624495983124,
0.025460422039031982,
-0.022341882809996605,
0.019878707826137543,
-0.019531110301613808,
0.012479068711400032,
0.021151769906282425,
0.0012046132469549775,
0.008840618655085564,
0.0328194834291935,
-0.0418907105922699,
0.011468899436295033,
0.08056894689798355,
-0.04640569910407066,
-0.008759279735386372,
-0.030419891700148582,
0.05042416602373123,
0.07275231182575226,
0.07885939627885818,
-0.006813790183514357,
0.041277650743722916,
-0.016458498314023018,
-0.01233243104070425,
0.06970010697841644,
-0.016562482342123985,
0.07401950657367706,
-0.12935328483581543,
0.016059547662734985,
0.04815686494112015,
-0.00908938143402338,
-0.05380038917064667,
-0.07282755523920059,
0.04511766508221626,
0.016698289662599564,
-0.06020362675189972,
0.04165571928024292,
0.022978587076067924,
-0.0012808219762519002,
0.007172210142016411,
-0.006541648413985968,
-0.02066541463136673,
-0.011058904230594635,
0.03359179571270943,
0.0017064010025933385,
0.05121779069304466,
0.017588574439287186,
-0.024668099358677864,
-0.035794056951999664,
0.023711809888482094,
-0.031924668699502945,
-0.011279265396296978,
-0.029221558943390846,
-0.04540286585688591,
0.01987561769783497,
-0.060230839997529984,
-0.06017805635929108,
0.02057541161775589,
0.012081030756235123,
-0.06841831654310226,
0.05221300572156906,
0.015203511342406273,
-0.011325424537062645,
-0.03518170863389969,
0.40061360597610474,
-0.04260661453008652,
-0.005884029436856508,
-0.011148436926305294,
-0.0254612248390913,
-0.057477306574583054,
-0.01075785979628563,
-0.021418852731585503,
-0.010296174325048923,
0.014049351215362549,
-0.005137147847563028,
0.07643283903598785,
0.05101153254508972,
0.07951559126377106,
-0.05429348349571228,
-0.06048530340194702,
-0.01338964607566595,
0.019654525443911552,
0.04522934928536415,
-0.017872361466288567,
0.06717267632484436,
0.009534443728625774,
0.060197778046131134,
0.07150005549192429,
0.034268077462911606,
-0.008372846059501171,
-0.02064402587711811,
0.007185375317931175,
0.08521328866481781,
0.05060451850295067,
-0.008394562639296055,
-0.014708326198160648,
0.047923848032951355,
-0.011517154984176159,
0.017679626122117043,
-0.007266712374985218,
-0.029101477935910225,
0.050562359392642975,
-0.03780495002865791,
0.0035325351636856794,
0.0029624763410538435,
-0.02177274040877819,
-0.007563890889286995,
-0.08875881135463715,
-0.015500162728130817,
-0.08326690644025803,
0.028188392519950867,
0.019122622907161713,
0.015797197818756104,
-0.014601098373532295,
0.0157843716442585,
-0.013728202320635319,
0.09452315419912338,
0.01188829354941845,
0.013925940729677677,
-0.025098873302340508,
0.014214384369552135,
-0.00007973916945047677,
-0.04612431675195694,
-0.009542528539896011,
0.024180196225643158,
-0.012161968275904655,
-0.0616409108042717,
-0.0026771225966513157,
0.06417986005544662,
0.010077087208628654,
-0.002956307725980878,
-0.06617636233568192,
-0.004285425413399935,
0.0249371025711298,
0.0385863333940506,
0.0005908094462938607,
0.04100704565644264,
-0.027364732697606087,
0.022980399429798126,
-0.04080783575773239,
-0.049004003405570984,
0.016761431470513344,
0.0461980402469635,
0.021148517727851868,
0.011431220918893814,
0.05300919711589813,
-0.02972712554037571,
-0.026726236566901207,
-0.025391217321157455,
0.04796111211180687,
-0.014533867128193378,
-0.06650347262620926,
0.019550349563360214,
-0.03647202253341675,
0.02033453807234764,
-0.06305409967899323,
-0.007444449700415134,
-0.051925383508205414,
-0.041422393172979355,
0.036186233162879944,
-0.006569043267518282,
-0.03233325108885765,
0.046375468373298645,
0.015864074230194092,
0.017559748142957687,
0.10105190426111221,
0.051584672182798386,
-0.007897459901869297,
0.047144778072834015,
-0.008208958432078362,
0.0004054640594404191,
0.03676118329167366,
0.04485036060214043,
0.020667629316449165,
0.01753419078886509,
-0.023351849988102913,
0.022721124812960625,
-0.0018058530986309052,
0.015965433791279793,
-0.058903150260448456,
0.09521260112524033,
-0.03536059334874153,
0.009588810615241528,
0.0663885548710823,
-0.0025291061028838158,
0.07009941339492798,
-0.014855396002531052,
-0.014978332445025444,
-0.3210231363773346,
-0.01942385733127594,
0.016581987962126732,
-0.026873193681240082,
0.004231040831655264,
-0.02452675625681877,
-0.048756059259176254,
-0.0664578378200531,
0.013179960660636425,
0.020910900086164474,
0.03316602110862732,
0.054746683686971664,
0.00416496442630887,
-0.005099726375192404,
-0.025565950199961662,
0.04479868337512016,
0.01719176024198532,
-0.0411238893866539,
-0.004654815420508385,
0.0037089299876242876,
0.025609223172068596,
-0.021951666101813316,
0.033925481140613556,
0.038605403155088425,
0.05620565637946129,
-0.04163379967212677,
0.1104462668299675,
0.018349550664424896,
0.03730850666761398,
-0.04553331807255745,
-0.02724568359553814,
0.10074494034051895,
-0.07610508054494858,
-0.08602769672870636,
-0.010608484968543053,
-0.0727185308933258,
-0.04866795986890793,
-0.057468775659799576,
-0.0385652631521225,
-0.07483765482902527,
0.011501388624310493,
0.05011002719402313,
0.005884196143597364,
-0.07476210594177246,
-0.04221543297171593,
-0.0025126938708126545,
-0.022578934207558632,
-0.007140648551285267,
0.055605143308639526,
-0.008621063083410263,
0.06330272555351257,
0.010351886972784996,
-0.043951380997896194,
0.005916276015341282,
-0.02721187099814415,
-0.00031682729604654014,
-0.05947522073984146,
-0.0033798515796661377,
-0.06595068424940109,
-0.044679444283246994,
-0.013294858857989311,
0.002246769843623042,
-0.02252401039004326,
-0.008031442761421204,
-0.04607817903161049,
0.012230896390974522,
0.04222077876329422,
-0.014512825757265091,
0.07533196359872818,
0.03987831249833107,
0.03863566741347313,
0.11170388013124466,
-0.020522231236100197,
0.11162075400352478,
0.05014878138899803,
-0.0017763401847332716,
-0.015472549945116043,
-0.08705595135688782,
-0.030899034813046455,
0.013451430015265942,
-0.041100189089775085,
-0.04623173549771309,
0.008150807581841946,
0.016747163608670235,
-0.0171032827347517,
0.012447023764252663,
0.053625207394361496,
-0.029731810092926025,
0.05373276770114899,
0.03229065239429474,
0.050958674401044846,
0.054891929030418396,
-0.004702375736087561,
-0.046378131955862045,
0.031169284135103226,
0.004365852102637291,
-0.2610122561454773,
0.0007285710307769477,
-0.023493574932217598,
-0.03468507528305054,
-0.03367293253540993,
0.05386252701282501,
0.028980620205402374,
-0.01590448059141636,
-0.00821248721331358,
0.0037763079162687063,
0.01886652037501335,
-0.020773041993379593,
0.05331709235906601,
0.022518722340464592,
-0.02282518707215786,
-0.05055738240480423,
0.01667880080640316,
-0.013536082580685616,
-0.011678008362650871,
-0.027780326083302498,
-0.0035914017353206873,
0.011493454687297344,
0.09516510367393494,
-0.008672955445945263,
-0.052858561277389526,
0.024079784750938416,
-0.007365452125668526,
0.04119138792157173,
-0.008677596226334572,
-0.014738999307155609,
0.0022329429630190134,
0.08105064183473587,
0.04919765144586563,
-0.00988299585878849,
0.07018955796957016,
-0.004667461849749088,
-0.03426884859800339,
-0.060177672654390335,
-0.005595909431576729,
0.017368897795677185,
-0.021842507645487785,
-0.005290605127811432,
-0.07622510939836502,
0.009034622460603714,
0.0523599348962307,
-0.09214387089014053,
-0.048912521451711655,
0.007661440409719944,
-0.012416360899806023,
0.02231729030609131,
-0.011519028805196285,
-0.0399014912545681,
0.07462295144796371,
0.02629528008401394,
-0.012941616587340832,
-0.014540434814989567,
0.030062753707170486,
-0.051662031561136246,
0.06023821234703064,
-0.03167015314102173,
-0.041603900492191315,
-0.01670217141509056,
-0.06044885516166687,
0.11954133957624435,
0.04419603571295738
] |
PMID:27137 | Stability of antibiotics and amino acids in two synthetic L-amino acid solutions commonly used for total parenteral nutrition in children. | The stability and interaction at 29 degrees C of ampicillin, carbenicillin, gentamicin, and polymyxin B were examined in a common electrolyte solution, invertose darrow, and in two synthetic l-amino acid solutions, one commercial (vamin with fructose; Vitrum) and the other a neonatal preparation modified for use in newborn infants. The concentration of amino acids was measured before and after the addition of these antibiotics. The concentration of antibiotics was measured over a 24-h period with a microbiological method. The concentration of ampicillin in invertose darrow fell 52%, and in vamin with fructose it fell 69%, whereas in the neonatal preparation the fall was only 22%. The concentration of carbenicillin in vamin with fructose fell 37%, and in the neonatal preparation it fell 31%. The combination of ampicillin or carbenicillin with gentamicin or polymyxin B did not influence the activity of the penicillins. The concentration of gentamicin and polymyxin B was unchanged in all solutions over a 24-h period. With the exception of cystine, the concentration of all amino acids remained constant after 24 h in the neonatal preparation with and without the different combinations of antibiotics. For cystine there was a fall of 20 to 30%. | Stability of antibiotics and amino acids in two synthetic L-amino acid solutions commonly used for total parenteral nutrition in children. The stability and interaction at 29 degrees C of ampicillin, carbenicillin, gentamicin, and polymyxin B were examined in a common electrolyte solution, invertose darrow, and in two synthetic l-amino acid solutions, one commercial (vamin with fructose; Vitrum) and the other a neonatal preparation modified for use in newborn infants. The concentration of amino acids was measured before and after the addition of these antibiotics. The concentration of antibiotics was measured over a 24-h period with a microbiological method. The concentration of ampicillin in invertose darrow fell 52%, and in vamin with fructose it fell 69%, whereas in the neonatal preparation the fall was only 22%. The concentration of carbenicillin in vamin with fructose fell 37%, and in the neonatal preparation it fell 31%. The combination of ampicillin or carbenicillin with gentamicin or polymyxin B did not influence the activity of the penicillins. The concentration of gentamicin and polymyxin B was unchanged in all solutions over a 24-h period. With the exception of cystine, the concentration of all amino acids remained constant after 24 h in the neonatal preparation with and without the different combinations of antibiotics. For cystine there was a fall of 20 to 30%. | [
-0.029516860842704773,
-0.03809517249464989,
0.01789553463459015,
-0.014178873039782047,
0.019720612093806267,
0.02181357890367508,
0.00012488587526604533,
0.018275368958711624,
-0.09658637642860413,
-0.03168092668056488,
0.016009487211704254,
-0.04411671310663223,
0.06395634263753891,
0.039828456938266754,
0.004560127854347229,
-0.0016997321508824825,
0.011141669936478138,
-0.027667410671710968,
-0.03551092743873596,
-0.024670574814081192,
0.003551796544343233,
0.009985243901610374,
0.049927689135074615,
-0.06112075597047806,
0.039200033992528915,
0.028428519144654274,
0.020499199628829956,
0.013112526386976242,
-0.025833219289779663,
-0.2467813938856125,
0.038617558777332306,
-0.026044681668281555,
0.02150568924844265,
-0.05347258225083351,
-0.0020981701090931892,
0.03840414807200432,
0.022458279505372047,
-0.026916615664958954,
-0.017848040908575058,
0.014719311147928238,
0.057896144688129425,
0.03121829964220524,
0.0016578895738348365,
0.04416252300143242,
-0.012082049623131752,
0.006881829351186752,
-0.048760853707790375,
0.0726858451962471,
0.1342410296201706,
0.013740339316427708,
-0.0008214995614252985,
-0.00949727464467287,
0.024372082203626633,
0.07999579608440399,
-0.02384093590080738,
0.002172448905184865,
0.007842342369258404,
-0.027870845049619675,
0.006704912520945072,
0.05765991285443306,
0.00046450088848359883,
0.016365740448236465,
-0.14723071455955505,
0.060731083154678345,
0.04104883596301079,
0.009218930266797543,
-0.03396739065647125,
-0.02314637042582035,
0.007323386613279581,
0.019123977050185204,
-0.03851935639977455,
0.018236758187413216,
0.051300570368766785,
0.07055467367172241,
0.0014246711507439613,
-0.014896164648234844,
0.010136505588889122,
-0.021999454125761986,
0.04431353136897087,
0.0035980793181806803,
-0.038297057151794434,
-0.004121450241655111,
-0.002543983282521367,
-0.06491288542747498,
-0.016376569867134094,
-0.009612075984477997,
0.00997441727668047,
0.006144994404166937,
0.06381730735301971,
0.04368772357702255,
-0.024273565039038658,
-0.0007361139869317412,
0.00888997782021761,
-0.008564339950680733,
-0.05676758289337158,
-0.005744479596614838,
-0.0466313436627388,
-0.018635226413607597,
0.004507811274379492,
0.3840963840484619,
-0.027319908142089844,
-0.0534939244389534,
-0.03776567429304123,
-0.03785740211606026,
-0.01163345854729414,
-0.021286308765411377,
0.01963888294994831,
-0.0016006685327738523,
0.04757096245884895,
0.011279692873358727,
0.014943721704185009,
0.030894601717591286,
0.05076207220554352,
-0.027628468349575996,
-0.015392983332276344,
0.04727939888834953,
0.06618142873048782,
-0.04350664094090462,
-0.020606517791748047,
-0.008747396990656853,
-0.014150151051580906,
-0.0064167785458266735,
0.0303025059401989,
-0.0030699875205755234,
0.0237977746874094,
-0.025693034753203392,
-0.020645014941692352,
0.04919919744133949,
0.01171540841460228,
0.03789467364549637,
0.024608556181192398,
0.016752539202570915,
-0.041649576276540756,
-0.043976664543151855,
-0.01819436252117157,
-0.01075847540050745,
-0.010091908276081085,
-0.03544892370700836,
0.04819478467106819,
-0.021306799724698067,
-0.056166984140872955,
-0.06415408849716187,
-0.0727768987417221,
-0.07511640340089798,
-0.038529232144355774,
0.053338997066020966,
0.012745638377964497,
0.05812995508313179,
0.021114420145750046,
-0.032289307564496994,
-0.03099556639790535,
0.06402889639139175,
0.016164349392056465,
0.007234979886561632,
0.03761227801442146,
0.04575401172041893,
-0.02602602168917656,
-0.0343666672706604,
-0.019632689654827118,
-0.006204765290021896,
-0.09698192775249481,
-0.06533648073673248,
-0.028593959286808968,
0.11152287572622299,
-0.08304201811552048,
0.016688155010342598,
0.010054687969386578,
0.005072479136288166,
0.01483088731765747,
-0.013204426504671574,
0.027606645599007607,
0.03664238005876541,
-0.02244904823601246,
-0.009961466304957867,
-0.04167310148477554,
0.0246956255286932,
0.001704342314042151,
-0.007072941400110722,
0.0550815612077713,
-0.017625316977500916,
0.00881548598408699,
0.024612559005618095,
-0.009834234602749348,
0.05522580444812775,
0.05916723608970642,
-0.012327617034316063,
-0.0669165849685669,
0.020037295296788216,
0.024513473734259605,
0.018189281225204468,
0.010076317004859447,
-0.014883319847285748,
-0.05429328978061676,
0.050454072654247284,
-0.02174476534128189,
0.006814797408878803,
-0.04943396523594856,
0.0013326826738193631,
0.07223017513751984,
0.019062276929616928,
0.03885357454419136,
0.04013660550117493,
0.018407296389341354,
0.063360795378685,
0.03611719608306885,
-0.011796485632658005,
0.0009567527449689806,
-0.03139277175068855,
0.029257647693157196,
0.011527931317687035,
-0.05388946458697319,
0.04383318871259689,
-0.04581039398908615,
-0.02277669869363308,
-0.0774250328540802,
0.03605268895626068,
-0.0045237671583890915,
-0.00022505721426568925,
-0.008877508342266083,
0.07014209032058716,
0.02280522510409355,
-0.0013287632027640939,
-0.017963171005249023,
-0.3007414937019348,
0.03521490842103958,
0.019327601417899132,
-0.01932411827147007,
0.11376781016588211,
-0.038528505712747574,
-0.021965395659208298,
-0.03733283653855324,
-0.05895987153053284,
-0.031161408871412277,
0.04896876588463783,
0.08716129511594772,
-0.05124319717288017,
-0.026713212952017784,
-0.0317697711288929,
-0.038931362330913544,
-0.011014281772077084,
-0.018515607342123985,
0.029567494988441467,
0.024754082784056664,
-0.03015327826142311,
-0.050911109894514084,
0.028663281351327896,
0.03941218554973602,
0.061540618538856506,
-0.016204169020056725,
0.10844588279724121,
-0.0336710549890995,
-0.007437175139784813,
-0.029550358653068542,
-0.006497542839497328,
0.08736193180084229,
-0.07615766674280167,
-0.06773235648870468,
-0.02437722682952881,
0.015517950057983398,
-0.016917703673243523,
-0.037470780313014984,
-0.0215490460395813,
0.00046210165601223707,
-0.04123412072658539,
0.08989034593105316,
0.00834907591342926,
-0.10904358327388763,
-0.05417656898498535,
-0.007478413637727499,
-0.0319809690117836,
-0.011842886917293072,
0.009873385541141033,
-0.0003410951467230916,
0.007802322972565889,
-0.005104680545628071,
0.02372198924422264,
0.01096496544778347,
-0.007855978794395924,
-0.02778690867125988,
0.00413604686036706,
-0.021532868966460228,
-0.06158789247274399,
-0.03123018704354763,
-0.039671726524829865,
0.01365214865654707,
-0.01893041841685772,
0.01033604983240366,
-0.025245098397135735,
0.027234390377998352,
0.03928256779909134,
-0.031720053404569626,
0.04041188582777977,
0.024305392056703568,
0.02778683789074421,
0.11266865581274033,
-0.06698549538850784,
0.019970301538705826,
0.018752461299300194,
0.08043509721755981,
-0.0010446335654705763,
-0.039599232375621796,
-0.10097984224557877,
0.003575083566829562,
-0.03797706589102745,
0.014081812463700771,
0.0474022775888443,
-0.005126995500177145,
0.023430394008755684,
0.013952972367405891,
-0.03821107745170593,
-0.001267181127332151,
0.08200729638338089,
-0.017283933237195015,
0.03405370935797691,
0.039784468710422516,
-0.006252394989132881,
-0.043540630489587784,
0.012661837972700596,
0.05348353460431099,
-0.2525619864463806,
0.05836246535181999,
-0.022868916392326355,
0.02088395319879055,
0.00812043808400631,
0.020775072276592255,
-0.016591941937804222,
-0.07042711973190308,
-0.01778622530400753,
0.009915540926158428,
0.00748325465247035,
-0.006150060798972845,
0.0911182388663292,
-0.009875686839222908,
0.012746096588671207,
0.005808185786008835,
0.054873742163181305,
-0.028090812265872955,
0.03685107082128525,
-0.01784425415098667,
0.034709230065345764,
-0.004041034262627363,
0.1506241261959076,
0.029808159917593002,
-0.01673787459731102,
0.013766007497906685,
-0.022775061428546906,
0.06047040596604347,
-0.03832494840025902,
0.026446402072906494,
-0.03644241765141487,
0.07132186740636826,
0.07166820764541626,
-0.002434628549963236,
0.10103245079517365,
-0.027817893773317337,
-0.09577088057994843,
-0.035384491086006165,
0.007065279874950647,
0.028932541608810425,
-0.007163602393120527,
-0.014701157808303833,
-0.08513665199279785,
-0.01114340778440237,
0.05821353569626808,
-0.03332015872001648,
0.03348767012357712,
-0.06808791309595108,
0.055070433765649796,
-0.018598143011331558,
-0.021556731313467026,
-0.01507113128900528,
0.03133202716708183,
-0.0022191700991243124,
0.046073783189058304,
-0.05414756014943123,
0.009029638953506947,
-0.0011501145781949162,
-0.041283901780843735,
-0.004333624616265297,
0.02505921572446823,
-0.006492431741207838,
-0.04611316695809364,
0.03645816072821617,
0.002356430049985647
] |
PMID:27138 | Chloramphenicol resistance in Streptococcus pneumoniae: enzymatic acetylation and possible plasmid linkage. | Clinical isolates of Streptococcus pneumoniae resistant to chloramphenicol were observed in France for the first time in 1973. During a 4-year survey, these strains were found to represent 6% of a total of 564 isolates of S. pneumoniae in a general hospital and to belong to 13 different serotypes. One such strain, referred to as BM 6001, was shown to inactivate chloramphenicol, and the process was found to be inducible. The inactivated products were demonstrated to be O-acetoxy esters of chloramphenicol. The synthesis of an inducible chloramphenicol acetyltransferase was shown to be responsible for the inactivation of the drug. The resistant strain was able to transfer the chloramphenicol marker by transformation to competent strains of pneumococci at a frequency of 1% of that observed for control chromosomal markers. The loss of resistance was enhanced by ethidium bromide treatment, but no chloramphenicol-resistant mutant was isolated by mutagenesis of a "cured" clone or naturally susceptible isolates. All attempts to isolate plasmid deoxyribonucleic acid as covalently closed circular molecules from strain BM 6001 have been unsuccessful, but epidemiological evidence and the fact that the genes specifying chloramphenicol acetyltransferase synthesis are usually located on plasmids suggest that this marker may be plasmid-borne in S. pneumoniae. | Chloramphenicol resistance in Streptococcus pneumoniae: enzymatic acetylation and possible plasmid linkage. Clinical isolates of Streptococcus pneumoniae resistant to chloramphenicol were observed in France for the first time in 1973. During a 4-year survey, these strains were found to represent 6% of a total of 564 isolates of S. pneumoniae in a general hospital and to belong to 13 different serotypes. One such strain, referred to as BM 6001, was shown to inactivate chloramphenicol, and the process was found to be inducible. The inactivated products were demonstrated to be O-acetoxy esters of chloramphenicol. The synthesis of an inducible chloramphenicol acetyltransferase was shown to be responsible for the inactivation of the drug. The resistant strain was able to transfer the chloramphenicol marker by transformation to competent strains of pneumococci at a frequency of 1% of that observed for control chromosomal markers. The loss of resistance was enhanced by ethidium bromide treatment, but no chloramphenicol-resistant mutant was isolated by mutagenesis of a "cured" clone or naturally susceptible isolates. All attempts to isolate plasmid deoxyribonucleic acid as covalently closed circular molecules from strain BM 6001 have been unsuccessful, but epidemiological evidence and the fact that the genes specifying chloramphenicol acetyltransferase synthesis are usually located on plasmids suggest that this marker may be plasmid-borne in S. pneumoniae. | [
-0.048581965267658234,
-0.03643945977091789,
-0.09414714574813843,
0.020345862954854965,
0.03110879473388195,
0.016901154071092606,
-0.021830877289175987,
0.038738496601581573,
-0.005773001350462437,
0.03619205206632614,
0.013880304992198944,
-0.042097438126802444,
-0.01327035017311573,
-0.06155918538570404,
-0.061122775077819824,
-0.023956753313541412,
-0.03378068655729294,
0.005402721464633942,
0.006168312393128872,
0.0044242157600820065,
0.009858747944235802,
0.014721814543008804,
0.04172506183385849,
0.0017120267730206251,
0.06064212694764137,
0.040514759719371796,
0.07601386308670044,
-0.02214537374675274,
-0.03979310393333435,
-0.20960791409015656,
0.04020843654870987,
-0.019129494205117226,
0.00040611979784443974,
-0.014400827698409557,
-0.01658083125948906,
0.018039453774690628,
0.0327109657227993,
0.020672881975769997,
0.02230815216898918,
0.04866943135857582,
0.05046360194683075,
0.026273222640156746,
0.020572980865836143,
0.018408935517072678,
-0.02397131361067295,
-0.011445418000221252,
-0.06293246150016785,
0.02758144401013851,
0.12138766050338745,
-0.0002748752012848854,
0.027445781975984573,
-0.03147686645388603,
-0.019874034449458122,
0.04917364940047264,
-0.023269204422831535,
-0.008906419388949871,
-0.002451210282742977,
0.027391521260142326,
0.02186424657702446,
-0.030715029686689377,
-0.016440903767943382,
0.06249106302857399,
-0.1585237681865692,
0.04051193222403526,
-0.022540537640452385,
0.06946641951799393,
0.013072888366878033,
-0.058087848126888275,
-0.0039906976744532585,
0.01902606151998043,
-0.06956732273101807,
-0.025855394080281258,
-0.04486573114991188,
0.012739616446197033,
0.047906674444675446,
0.023566070944070816,
-0.02775527723133564,
-0.0005067216698080301,
0.02997160702943802,
-0.03403259068727493,
0.005821782164275646,
-0.05808284878730774,
-0.0027166290674358606,
-0.0012470628134906292,
-0.06255767494440079,
0.02618975192308426,
0.024887315928936005,
-0.06010529026389122,
0.017844876274466515,
0.030408745631575584,
-0.016803056001663208,
-0.019578706473112106,
0.012813951820135117,
0.02122769132256508,
-0.07953395694494247,
0.0011723014758899808,
0.03756875917315483,
0.027346894145011902,
0.007105876225978136,
0.35507625341415405,
-0.07629694789648056,
-0.008147165179252625,
-0.07172485440969467,
-0.020140109583735466,
0.00250150915235281,
-0.026073861867189407,
0.006192868109792471,
0.023532874882221222,
0.02505563199520111,
0.024882275611162186,
-0.010134441778063774,
-0.007227215450257063,
0.015415291301906109,
-0.041680168360471725,
-0.007078235503286123,
-0.044872213155031204,
0.047488849610090256,
0.024384254589676857,
-0.050679005682468414,
0.06590195000171661,
0.019729984924197197,
-0.027528513222932816,
0.042023200541734695,
-0.015260253101587296,
0.019288571551442146,
0.07347192615270615,
-0.028231831267476082,
0.07886480540037155,
0.0802895650267601,
-0.04061103239655495,
0.012938846834003925,
-0.008145764470100403,
0.009226980619132519,
-0.02525996044278145,
-0.009136123582720757,
-0.02752654068171978,
0.005543098319321871,
-0.08484657108783722,
0.0752050131559372,
-0.008871888741850853,
0.0414627343416214,
-0.06891130656003952,
0.019167039543390274,
-0.04991251602768898,
-0.049834687262773514,
0.0767410472035408,
-0.05790766701102257,
0.014609175734221935,
-0.03301341086626053,
-0.01516422163695097,
0.03929620236158371,
0.06662775576114655,
-0.022842520847916603,
0.003947904799133539,
0.029766691848635674,
0.08800792694091797,
0.017865892499685287,
0.02747071348130703,
0.01844916306436062,
0.06427685171365738,
-0.056876856833696365,
-0.010850110091269016,
-0.03198841214179993,
0.07813437283039093,
-0.018531296402215958,
-0.06322341412305832,
-0.01568400301039219,
0.07645154744386673,
0.04049518704414368,
0.023333141580224037,
0.008755707181990147,
-0.003388327546417713,
-0.011376997455954552,
-0.009886114858090878,
-0.06081727147102356,
0.050761159509420395,
-0.0038948592264205217,
-0.045210208743810654,
0.007420077454298735,
0.057149115949869156,
-0.007260041311383247,
-0.0012733906041830778,
0.03653604909777641,
-0.002498748479411006,
-0.003965636715292931,
-0.024666212499141693,
-0.01743987388908863,
0.005825186148285866,
0.004665956366807222,
0.05319349467754364,
-0.0077529363334178925,
0.04960581287741661,
-0.06722327321767807,
0.029447566717863083,
0.04361603409051895,
-0.014371662400662899,
-0.053520869463682175,
-0.006215066649019718,
0.04737929254770279,
-0.027454940602183342,
0.06728055328130722,
0.07066939026117325,
0.026898080483078957,
0.012488803826272488,
0.007727273274213076,
-0.011513372883200645,
0.03512071073055267,
-0.02966913767158985,
0.010192383080720901,
0.016027171164751053,
-0.031014442443847656,
0.005906866397708654,
-0.03990313410758972,
0.060981474816799164,
-0.060355424880981445,
0.003912128508090973,
-0.029358074069023132,
-0.046594925224781036,
0.014018838293850422,
-0.016554279252886772,
0.03239017724990845,
0.004439329728484154,
0.008166613057255745,
-0.3081379532814026,
-0.03577347844839096,
0.0013344890903681517,
0.042170360684394836,
0.012770900502800941,
-0.003945354837924242,
0.02486444264650345,
0.0005335224559530616,
-0.009158879518508911,
-0.07984653115272522,
0.04933566972613335,
0.07607901841402054,
-0.058481454849243164,
-0.03878297284245491,
0.01819271594285965,
0.03938990458846092,
0.018975403159856796,
-0.037271562963724136,
0.005330780986696482,
-0.054864078760147095,
0.06468226760625839,
-0.02921496517956257,
-0.0357208251953125,
0.024998864158988,
0.0646386370062828,
0.00016307791520375758,
0.1257801204919815,
-0.03094569221138954,
0.026185905560851097,
0.03704110532999039,
0.01783236488699913,
0.023032667115330696,
-0.0013901229249313474,
-0.04457530751824379,
-0.04593911021947861,
-0.022609738633036613,
-0.05336272716522217,
-0.025624055415391922,
0.07609903067350388,
0.011577405966818333,
0.024863993749022484,
-0.01245594397187233,
-0.014796331524848938,
-0.045096591114997864,
-0.04368177428841591,
-0.017190193757414818,
-0.011323194019496441,
0.034962400794029236,
-0.04170476272702217,
0.04248073697090149,
-0.04348890110850334,
0.012511362321674824,
0.03711988031864166,
0.0515323169529438,
-0.025277098640799522,
0.003711399622261524,
-0.025623759254813194,
0.00610328046604991,
0.0058115823194384575,
-0.03150060027837753,
-0.028661679476499557,
0.025733191519975662,
0.04751968011260033,
-0.04491937905550003,
0.005397528875619173,
-0.037887897342443466,
0.006814911030232906,
-0.06006162241101265,
0.041922107338905334,
0.05093882977962494,
-0.03979334980249405,
0.12589283287525177,
-0.03692322596907616,
-0.04849441349506378,
0.053641222417354584,
0.07576239109039307,
-0.053802240639925,
-0.0529433935880661,
-0.11943797022104263,
0.030642803758382797,
-0.035659726709127426,
-0.0788949579000473,
-0.02191012166440487,
0.00742368446663022,
0.01058858074247837,
0.015581933781504631,
0.012100586667656898,
0.01586705632507801,
0.006231300067156553,
0.0069715166464447975,
-0.016884827986359596,
-0.03163256496191025,
0.002964253770187497,
-0.07059449702501297,
-0.014382977969944477,
-0.003460219595581293,
-0.24435919523239136,
-0.001893295207992196,
0.051274944096803665,
0.08500125259160995,
0.008231792598962784,
0.06344809383153915,
0.04561854153871536,
-0.07788802683353424,
0.014933251775801182,
-0.01306653767824173,
0.06898380070924759,
0.006777401082217693,
0.06647191196680069,
0.03608204424381256,
-0.010857454501092434,
0.016084564849734306,
0.04132387042045593,
-0.03306858241558075,
0.04915482550859451,
0.017798371613025665,
-0.012824885547161102,
-0.057378850877285004,
0.11796417087316513,
0.10264008492231369,
-0.02584521472454071,
0.035706643015146255,
0.015204018913209438,
0.05692237243056297,
0.01390918530523777,
0.018359940499067307,
0.018824441358447075,
-0.021389558911323547,
0.0703955888748169,
-0.05420641228556633,
0.03646162897348404,
-0.005782060790807009,
0.0032674844842404127,
-0.029722614213824272,
-0.03588898479938507,
-0.027039997279644012,
-0.05220084264874458,
-0.04014509916305542,
-0.06693454831838608,
-0.07363644987344742,
0.0846036821603775,
-0.07254905998706818,
-0.03578919544816017,
-0.09837289899587631,
0.027652205899357796,
0.024534404277801514,
-0.036946289241313934,
0.0007591555477119982,
0.025287970900535583,
-0.013833451084792614,
-0.046067241579294205,
-0.020778756588697433,
-0.024677185341715813,
-0.0453970804810524,
0.04200204461812973,
0.019459450617432594,
0.006763008423149586,
-0.027198512107133865,
-0.020139791071414948,
0.09451103210449219,
0.04935101419687271
] |
PMID:27139 | Irreversible effects of serum proteins on beta-lactam antibiotics. | The chromogenic cephalosporin nitrocefin (87/312) demonstrates rapid and visible instability to serum from many species. This phenomenon was distinct from serum binding, being significantly slower. Destruction of another cephalosporin, 10485, by serum appeared to account for some anomalous results during investigation into its human pharmacokinetics. Many cephalosporins of very different structures also showed serum instability, unrelated to their degrees of serum binding as measured by plate assay. Extrapolation could not be made from one species to another with regard to either binding or instability. Small changes in the chemical structures of the 3- and 7-substituents of the cephalosporins made profound changes in their susceptibility to serum attack. The decomposition is pH dependent, occurring more slowly at acid pH, and is due to a high-molecular-weight component of serum that resists boiling for several minutes. Isoelectric focusing of serum from several animal species gave various species-specific bands that decomposed nitrocefin. The inactivation of nitrocefin was not entirely parallel with that of 10485 and was inhibited by it. All other beta-lactam compounds tested also inhibited the reaction, much greater concentrations usually being necessary when the inhibitor was stable to serum. The complex that causes breakdown of the beta-lactam compounds is not necessarily the same as the one causing serum binding. It is postulated that serum may affect most other beta-lactam antibiotics in a similar way, although in most cases, this only occurs to a very slight extent. | Irreversible effects of serum proteins on beta-lactam antibiotics. The chromogenic cephalosporin nitrocefin (87/312) demonstrates rapid and visible instability to serum from many species. This phenomenon was distinct from serum binding, being significantly slower. Destruction of another cephalosporin, 10485, by serum appeared to account for some anomalous results during investigation into its human pharmacokinetics. Many cephalosporins of very different structures also showed serum instability, unrelated to their degrees of serum binding as measured by plate assay. Extrapolation could not be made from one species to another with regard to either binding or instability. Small changes in the chemical structures of the 3- and 7-substituents of the cephalosporins made profound changes in their susceptibility to serum attack. The decomposition is pH dependent, occurring more slowly at acid pH, and is due to a high-molecular-weight component of serum that resists boiling for several minutes. Isoelectric focusing of serum from several animal species gave various species-specific bands that decomposed nitrocefin. The inactivation of nitrocefin was not entirely parallel with that of 10485 and was inhibited by it. All other beta-lactam compounds tested also inhibited the reaction, much greater concentrations usually being necessary when the inhibitor was stable to serum. The complex that causes breakdown of the beta-lactam compounds is not necessarily the same as the one causing serum binding. It is postulated that serum may affect most other beta-lactam antibiotics in a similar way, although in most cases, this only occurs to a very slight extent. | [
-0.07030641287565231,
0.011428559198975563,
0.021615728735923767,
0.026474781334400177,
0.009202150627970695,
-0.017937438562512398,
0.08381908386945724,
0.0529610812664032,
-0.05802648141980171,
-0.04853411018848419,
-0.010569814592599869,
-0.017192423343658447,
0.026879621669650078,
0.017476869747042656,
-0.04243179038167,
-0.021250568330287933,
0.008474025875329971,
-0.03304493799805641,
-0.050694793462753296,
0.06233075261116028,
-0.04113287106156349,
0.02092021517455578,
0.03400707244873047,
0.03455952927470207,
0.04281245544552803,
0.04658205062150955,
0.008122791536152363,
0.017600620165467262,
-0.07702811062335968,
-0.24023129045963287,
0.03242826089262962,
-0.02935784123837948,
0.005555210169404745,
-0.03550897166132927,
0.0011836718767881393,
0.049057070165872574,
-0.03956005722284317,
0.025697708129882812,
0.043156567960977554,
-0.008497553877532482,
0.0697493776679039,
0.03663952648639679,
-0.00904910359531641,
0.038198813796043396,
0.06000465899705887,
-0.017166268080472946,
-0.01742316037416458,
0.053430452942848206,
0.11345445364713669,
0.03724377974867821,
0.007034710608422756,
-0.03836081549525261,
0.04182521253824234,
0.09031371027231216,
0.010418260470032692,
0.016286473721265793,
-0.022738398984074593,
0.020402632653713226,
0.039895325899124146,
0.020417345687747,
-0.0025910057593137026,
0.003797488287091255,
-0.11971660703420639,
0.011964539997279644,
0.007188949268311262,
0.037856366485357285,
0.0036783546674996614,
-0.07593874633312225,
0.04996895045042038,
0.06145903095602989,
-0.012501844204962254,
-0.0007469366537407041,
-0.016704484820365906,
-0.01569652557373047,
0.014511596411466599,
-0.019346680492162704,
-0.0066293710842728615,
0.017239559441804886,
0.00966414250433445,
0.016817666590213776,
0.005968857556581497,
-0.02915322221815586,
0.008932448923587799,
-0.04345506429672241,
0.041880954056978226,
-0.018562305718660355,
0.06845460832118988,
-0.0024864920414984226,
0.011093120090663433,
0.01896190084517002,
-0.046045687049627304,
-0.0645126923918724,
0.04431457817554474,
-0.026488587260246277,
-0.04456998035311699,
-0.022613724693655968,
0.007379933726042509,
0.003220814513042569,
0.013001126237213612,
0.33576130867004395,
-0.014961767010390759,
-0.03095591813325882,
-0.07854017615318298,
0.031022755429148674,
0.020840702578425407,
0.029733313247561455,
0.05961693078279495,
0.0016702499706298113,
0.013624714687466621,
0.019060537219047546,
0.014728195033967495,
0.013699017465114594,
0.0464421771466732,
-0.07945450395345688,
0.013276978395879269,
0.028139697387814522,
0.03590429946780205,
0.006508865859359503,
-0.08081232756376266,
0.019861215725541115,
0.023391464725136757,
0.0428847037255764,
0.04236753657460213,
-0.02824275568127632,
0.07025231420993805,
0.02737555280327797,
-0.016185559332370758,
0.0844925045967102,
0.06169898062944412,
-0.056443918496370316,
0.011933650821447372,
-0.03694811463356018,
0.0006802549469284713,
0.005704319104552269,
0.0007467888062819839,
0.0033358801156282425,
0.01744016818702221,
-0.028088785707950592,
0.05933932587504387,
0.03300492465496063,
-0.012022369541227818,
-0.0371071919798851,
-0.05836864188313484,
-0.05664118379354477,
-0.0441475585103035,
0.12364932894706726,
0.0017692127730697393,
0.023981206119060516,
-0.018138667568564415,
-0.012472761794924736,
-0.054810333997011185,
0.019702518358826637,
-0.021568741649389267,
0.0358702577650547,
-0.041048791259527206,
0.016022857278585434,
0.018544647842645645,
-0.0009307293221354485,
-0.06379914283752441,
0.0031933262944221497,
-0.10837024450302124,
-0.014776661060750484,
-0.011467978358268738,
0.05293066427111626,
-0.03614842891693115,
0.01813478395342827,
0.015848912298679352,
-0.014735388569533825,
-0.048923734575510025,
-0.03806138038635254,
-0.057975731790065765,
-0.023998983204364777,
0.004742668010294437,
-0.02236953377723694,
-0.02379390224814415,
0.022521821781992912,
-0.0018081343732774258,
-0.005250618327409029,
0.04850771278142929,
-0.012010501697659492,
0.02862917259335518,
0.06875117868185043,
-0.013882394880056381,
0.08055315166711807,
0.0006681103259325027,
-0.016609592363238335,
-0.05272199586033821,
0.03296531364321709,
0.034909553825855255,
0.05382022634148598,
-0.04672243818640709,
-0.007736198138445616,
-0.06657111644744873,
0.054639559239149094,
-0.07131985574960709,
-0.009881868958473206,
-0.023504404351115227,
-0.009206317365169525,
-0.02496470883488655,
-0.035136628895998,
0.08612813800573349,
0.018483586609363556,
0.04211561009287834,
0.045778971165418625,
0.02448110282421112,
0.048934489488601685,
-0.018808182328939438,
-0.01944836787879467,
0.022172050550580025,
-0.024674635380506516,
0.003805811982601881,
0.03029940277338028,
-0.08621349930763245,
-0.018734049052000046,
-0.07812085002660751,
0.031170684844255447,
-0.04351372644305229,
0.01488989032804966,
0.011549261398613453,
0.00835203193128109,
0.05865800380706787,
-0.03666731342673302,
-0.044744301587343216,
-0.3068481385707855,
0.020534001290798187,
0.01840262860059738,
-0.04595024883747101,
0.042875707149505615,
-0.04782342165708542,
-0.030988218262791634,
-0.015539475716650486,
-0.027777304872870445,
-0.06346997618675232,
0.03934617340564728,
0.07526922225952148,
-0.04052693769335747,
-0.03776618465781212,
0.03401993587613106,
0.010315146297216415,
0.006190132815390825,
-0.034159105271101,
0.03982153162360191,
-0.015615791082382202,
-0.032566603273153305,
-0.010175981558859348,
0.011941245757043362,
0.044350169599056244,
0.038650091737508774,
-0.028486063703894615,
0.06968814134597778,
-0.030930455774068832,
-0.019700556993484497,
-0.02031909115612507,
-0.040096089243888855,
0.07755210995674133,
-0.06113841384649277,
-0.04726415500044823,
-0.009753362275660038,
0.03279140591621399,
0.040887679904699326,
-0.047422248870134354,
-0.01802750676870346,
0.007415481377393007,
-0.015635788440704346,
-0.006411741487681866,
0.004321317188441753,
0.0030872393399477005,
-0.04340380057692528,
-0.007459373213350773,
-0.01851627044379711,
0.06377998739480972,
0.02281840890645981,
0.045179881155490875,
0.02639203518629074,
-0.02242979407310486,
-0.0116864163428545,
0.04047500714659691,
0.04875989258289337,
0.027452483773231506,
-0.05073273926973343,
0.025888977572321892,
-0.024943433701992035,
-0.010048376396298409,
0.049261365085840225,
-0.0026879101060330868,
0.04385802149772644,
0.01945524290204048,
0.01795542798936367,
-0.050387512892484665,
-0.0040512485429644585,
-0.046622972935438156,
0.044568248093128204,
0.009840116836130619,
-0.0047225248999893665,
0.15074320137500763,
-0.047918595373630524,
-0.007384158205240965,
0.08765419572591782,
0.06457484513521194,
-0.06738956272602081,
-0.06707420200109482,
-0.04940391331911087,
0.0285827424377203,
0.026715071871876717,
-0.039318013936281204,
0.01000741496682167,
-0.0033274581655859947,
0.04562648758292198,
-0.0028012809343636036,
0.0198049359023571,
0.01900027133524418,
0.03364026919007301,
0.011950809508562088,
-0.011720082722604275,
0.04054306820034981,
-0.04503441974520683,
-0.04993220046162605,
-0.015118489973247051,
0.06462465226650238,
-0.2815565764904022,
-0.009922176599502563,
0.09618968516588211,
0.019459454342722893,
0.0304490365087986,
0.06280617415904999,
0.022185076028108597,
-0.07423150539398193,
-0.0580892339348793,
-0.013691449537873268,
-0.008315474726259708,
0.029736926779150963,
0.051465924829244614,
0.03070186823606491,
-0.01551112625747919,
-0.010788965970277786,
0.05706492438912392,
-0.061151742935180664,
0.021239908412098885,
-0.03894399479031563,
0.0038015171885490417,
0.02054072730243206,
0.16112208366394043,
0.019418854266405106,
-0.006371581461280584,
0.01624530367553234,
0.014146193861961365,
0.07099225372076035,
-0.0352725125849247,
0.0248058270663023,
0.007752635981887579,
0.0027652792632579803,
0.03649795800447464,
-0.05853383615612984,
-0.017155203968286514,
-0.0013273671502247453,
-0.020482558757066727,
-0.061456747353076935,
-0.029789967462420464,
-0.021224986761808395,
0.006531685125082731,
0.04124816879630089,
-0.03235728293657303,
-0.03316983953118324,
0.045147329568862915,
-0.08236736804246902,
-0.06375540792942047,
-0.005919665098190308,
0.026725616306066513,
-0.008115855045616627,
-0.062027547508478165,
-0.04561542347073555,
-0.0013711110223084688,
-0.012837029062211514,
0.02179827354848385,
-0.040973011404275894,
-0.056053098291158676,
-0.00982348620891571,
-0.02555375173687935,
-0.0387398824095726,
-0.0024097005371004343,
0.023738160729408264,
-0.1345061957836151,
0.04509530961513519,
0.031560663133859634
] |
PMID:27140 | Antimicrobial susceptibility patterns of Streptococcus pneumoniae. | Fifty clinical isolates of Streptococcus pneumoniae received by the Streptococcus Laboratory of the Center for Disease Control from August 1976 through March 1977 and 50 pneumococcal strains retrieved from 13- to 16-year storage (originally isolated October 1961 through December 1964) were tested for susceptibility to 10 antimicrobial agents by disk-agar diffusion and agar dilution tests. No appreciable differences in susceptibility patterns were apparent between the two groups, and, except for one isolate, all were highly susceptible to every drug tested except gentamicin. This single isolate required higher drug concentrations to inhibit macroscopic growth and had corresponding decrements in zones of disk inhibition with penicillin, ampicillin, and cephalothin. An additional 43 pneumococci recently received from various areas of the United States and Canada were screened by a disk agar diffusion method for penicillin resistance. Four of these isolates had penicillin zone diameters <30 mm, and subsequent agar dilution test results showed that the penicillin minimum inhibitory concentrations were elevated with these organisms. Antimicrobial susceptibility patterns of pneumococci to antimicrobials other than penicillin and its analogs have not changed substantially in over a decade. However, due to the emergence of strains with decreased susceptibility to penicillin, the screening test for penicillin resistance in pneumococci, especially in isolates from spinal fluid and blood, could be clinically useful as an aid in selecting optimal therapy. | Antimicrobial susceptibility patterns of Streptococcus pneumoniae. Fifty clinical isolates of Streptococcus pneumoniae received by the Streptococcus Laboratory of the Center for Disease Control from August 1976 through March 1977 and 50 pneumococcal strains retrieved from 13- to 16-year storage (originally isolated October 1961 through December 1964) were tested for susceptibility to 10 antimicrobial agents by disk-agar diffusion and agar dilution tests. No appreciable differences in susceptibility patterns were apparent between the two groups, and, except for one isolate, all were highly susceptible to every drug tested except gentamicin. This single isolate required higher drug concentrations to inhibit macroscopic growth and had corresponding decrements in zones of disk inhibition with penicillin, ampicillin, and cephalothin. An additional 43 pneumococci recently received from various areas of the United States and Canada were screened by a disk agar diffusion method for penicillin resistance. Four of these isolates had penicillin zone diameters <30 mm, and subsequent agar dilution test results showed that the penicillin minimum inhibitory concentrations were elevated with these organisms. Antimicrobial susceptibility patterns of pneumococci to antimicrobials other than penicillin and its analogs have not changed substantially in over a decade. However, due to the emergence of strains with decreased susceptibility to penicillin, the screening test for penicillin resistance in pneumococci, especially in isolates from spinal fluid and blood, could be clinically useful as an aid in selecting optimal therapy. | [
-0.018839513882994652,
-0.01871596835553646,
-0.05102647840976715,
-0.002420586533844471,
0.06268655508756638,
0.04843343794345856,
-0.020603947341442108,
0.044711556285619736,
-0.054110221564769745,
0.026408212259411812,
-0.011990819126367569,
-0.02504293993115425,
0.039477936923503876,
-0.020164700224995613,
-0.04873961582779884,
-0.02175198681652546,
-0.03665382042527199,
-0.03505716845393181,
-0.04450073838233948,
0.004250787198543549,
-0.035475362092256546,
0.0491812601685524,
-0.013333864510059357,
-0.08513879030942917,
0.05922245234251022,
0.02895403653383255,
0.05748198181390762,
-0.011239312589168549,
-0.048421453684568405,
-0.2211780697107315,
0.03373827412724495,
0.002951225498691201,
-0.006940761115401983,
-0.06158754974603653,
-0.011964705772697926,
-0.0018057990819215775,
0.01787254959344864,
-0.005752902943640947,
-0.000663303304463625,
0.017114784568548203,
0.041547082364559174,
0.059027332812547684,
0.05887211114168167,
0.05155741795897484,
-0.051154494285583496,
-0.03675801306962967,
-0.09149220585823059,
0.0459248311817646,
0.15068049728870392,
0.007357309572398663,
-0.0022244511637836695,
-0.038772013038396835,
-0.012982126325368881,
0.062117498368024826,
-0.03879429027438164,
-0.06744195520877838,
-0.02965780906379223,
-0.012385333888232708,
-0.01108634378761053,
0.0021792834158986807,
-0.018445009365677834,
0.03216215968132019,
-0.13332393765449524,
0.033427055925130844,
0.011430135928094387,
0.026023009791970253,
0.007790172938257456,
-0.0413520447909832,
0.008083698339760303,
0.00156541564501822,
-0.02011299692094326,
0.03527573123574257,
-0.06512010097503662,
0.0851886123418808,
0.024456866085529327,
0.01946871168911457,
-0.051994793117046356,
-0.043532323092222214,
0.044741787016391754,
0.06225435435771942,
-0.03860306367278099,
0.021043768152594566,
0.011062055826187134,
0.016232220456004143,
-0.05625559762120247,
-0.03356187045574188,
0.023236632347106934,
-0.03945264220237732,
0.03350581228733063,
0.024940624833106995,
-0.017148097977042198,
0.01169615425169468,
0.020992452278733253,
-0.007374785374850035,
-0.0993008241057396,
-0.06539572775363922,
0.01926281675696373,
-0.0021316607017070055,
-0.005933019332587719,
0.3812333941459656,
-0.03786991536617279,
-0.052237704396247864,
-0.03656651824712753,
0.04321027919650078,
-0.002289807889610529,
-0.005774406250566244,
0.07973744720220566,
-0.06752832978963852,
0.026635589078068733,
0.032051052898168564,
0.019786659628152847,
0.0006305832066573203,
0.030256280675530434,
-0.03600800037384033,
-0.027185335755348206,
-0.006091488059610128,
0.04314364120364189,
-0.009762587957084179,
-0.0385490357875824,
-0.020027615129947662,
-0.028017165139317513,
-0.038105159997940063,
0.005303439684212208,
-0.045315295457839966,
0.007658146787434816,
0.017953209578990936,
-0.034477319568395615,
0.059512075036764145,
0.0404835045337677,
-0.0435870997607708,
0.04677630960941315,
-0.01708901673555374,
-0.01711256429553032,
-0.017699364572763443,
0.016772354021668434,
-0.01577984355390072,
-0.013304454274475574,
-0.010960506275296211,
0.07309609651565552,
0.04842142015695572,
-0.01888725906610489,
-0.05802612751722336,
0.020597774535417557,
-0.02118776924908161,
-0.03545044735074043,
0.07387280464172363,
-0.03821346536278725,
0.03755894675850868,
-0.014130947180092335,
-0.06008472666144371,
0.05217238515615463,
0.08912365883588791,
-0.017688201740384102,
-0.032636404037475586,
0.024608945474028587,
0.016188492998480797,
0.008271932601928711,
-0.015311029739677906,
-0.004717565607279539,
0.01967405341565609,
-0.061126742511987686,
0.009654426015913486,
-0.012158999219536781,
0.1382492035627365,
-0.008960186503827572,
-0.039812617003917694,
0.03528041020035744,
-0.009356621652841568,
0.044996265321969986,
0.01107639167457819,
0.04113265499472618,
0.03655492141842842,
-0.006716524716466665,
-0.02333672158420086,
-0.08265268802642822,
0.03627866879105568,
-0.014277918264269829,
-0.058984383940696716,
0.03760414198040962,
0.01995617337524891,
0.004684878513216972,
0.07208660244941711,
-0.04969070106744766,
0.03434104099869728,
0.05229692906141281,
0.033402346074581146,
-0.06276031583547592,
0.026895392686128616,
0.0549052469432354,
0.013023565523326397,
-0.0008028021547943354,
-0.036704763770103455,
-0.08276766538619995,
0.0068811713717877865,
-0.019132252782583237,
0.0059450538828969,
-0.047246791422367096,
0.03475544601678848,
0.029661312699317932,
-0.04055866599082947,
0.03354073688387871,
0.028150467202067375,
0.02925272472202778,
0.018501725047826767,
0.02745727449655533,
0.02010846510529518,
0.009920336306095123,
-0.016140742227435112,
0.004258628934621811,
0.024184970185160637,
-0.06964536011219025,
0.01739474944770336,
-0.05482729524374008,
0.030210793018341064,
-0.06221882998943329,
0.038959700614213943,
-0.014502402395009995,
-0.02396412193775177,
0.025247113779187202,
0.005799867212772369,
0.01630130596458912,
0.03566589578986168,
-0.0005510315531864762,
-0.3162934482097626,
0.01695909909904003,
0.019657421857118607,
0.019411807879805565,
0.07631344348192215,
0.005423463881015778,
0.0038299751468002796,
-0.005868838168680668,
-0.001603781129233539,
-0.03740162402391434,
0.015872983261942863,
0.07416808605194092,
-0.0826193168759346,
-0.018886879086494446,
0.0009969439124688506,
0.004603056237101555,
-0.00019382692698854953,
-0.019322004169225693,
-0.006051021162420511,
-0.05248754471540451,
0.026845397427678108,
-0.010158412158489227,
0.006447444204241037,
0.06447198241949081,
0.04267466440796852,
0.007861979305744171,
0.09326427429914474,
-0.06684519350528717,
0.0006130745750851929,
0.016872797161340714,
0.025460844859480858,
0.03618936985731125,
-0.012742744758725166,
-0.05346032232046127,
-0.02072296477854252,
-0.000338948069838807,
0.023140864446759224,
-0.028528140857815742,
0.012625981122255325,
-0.036572135984897614,
-0.04193499684333801,
0.08344022184610367,
-0.03597685694694519,
-0.04341176897287369,
-0.07415733486413956,
-0.029922205954790115,
0.02156110107898712,
0.0688362717628479,
-0.024227293208241463,
0.019897766411304474,
-0.03758196160197258,
0.018019570037722588,
0.008266875520348549,
0.055835288017988205,
0.008042199537158012,
-0.008995185606181622,
0.003900270676240325,
-0.03820859268307686,
0.006581661757081747,
-0.058392275124788284,
-0.026572223752737045,
0.01651838980615139,
0.05078812316060066,
-0.020317474380135536,
-0.0382470078766346,
-0.005829493049532175,
-0.01311079878360033,
-0.04140624776482582,
0.05738413706421852,
0.08532097935676575,
0.04246630519628525,
0.08275042474269867,
-0.061949167400598526,
-0.037785157561302185,
0.06438182294368744,
0.0609414279460907,
-0.008719565346837044,
-0.00979969184845686,
-0.09507384151220322,
0.015201043337583542,
0.0065363491885364056,
-0.03348517790436745,
0.009842971339821815,
0.008141611702740192,
0.04273533448576927,
0.01274967286735773,
-0.003985688090324402,
-0.005123256240040064,
0.06428895145654678,
-0.04491649940609932,
0.0004253116494510323,
-0.03424781560897827,
-0.022820966318249702,
-0.06761755794286728,
0.050734393298625946,
0.04093283414840698,
-0.26103073358535767,
0.004687949549406767,
0.05978825315833092,
0.060855936259031296,
0.02205233834683895,
0.02390463463962078,
0.021672358736395836,
-0.026571080088615417,
0.025248292833566666,
-0.027068059891462326,
0.03721235692501068,
0.0651320144534111,
0.06344374269247055,
0.012560243718326092,
-0.012338116765022278,
-0.004914252087473869,
0.05848744884133339,
-0.03391341492533684,
0.07287390530109406,
0.0009379140101373196,
0.039968036115169525,
-0.013266167603433132,
0.1346488744020462,
-0.026896869763731956,
-0.014342869631946087,
0.0005258655291981995,
0.029077326878905296,
0.021901540458202362,
-0.026838228106498718,
0.034046292304992676,
0.03098909556865692,
-0.020449649542570114,
0.0672258585691452,
-0.007830487564206123,
0.078071229159832,
0.033914025872945786,
0.004932814743369818,
-0.02399394102394581,
0.009687786921858788,
-0.04545372724533081,
-0.012113271281123161,
-0.018780210986733437,
-0.0650940090417862,
-0.0028545577079057693,
0.06320901215076447,
-0.07615724951028824,
0.021577375009655952,
-0.0641876682639122,
0.009699549525976181,
0.016171174123883247,
-0.03374960273504257,
-0.05282049626111984,
0.023179683834314346,
-0.017861738801002502,
0.02085038274526596,
-0.026897862553596497,
-0.003897497896105051,
-0.05096995458006859,
-0.007630940061062574,
-0.041158974170684814,
0.027355091646313667,
-0.05742555856704712,
-0.028665069490671158,
0.01979636587202549,
0.025385215878486633
] |
PMID:27141 | Susceptibility of skin and throat strains of group A streptococci to rosamicin and erythromycin. | The minimal inhibitory concentrations of rosamicin and erythromycin were compared for 210 strains of group A streptococcus isolated from a diverse spectrum of streptococcal diseases. Of these strains, 97.6% were inhibited by 0.1 to 0.2 mug of rosamicin per ml, whereas 90.9% were inhibited by 0.025 to 0.05 mug of erythromycin per ml. The minimal inhibitory concentration of rosamicin for 154 strains exceeded that of erythromycin by at least fourfold. Five group A strains of streptococcus that were highly resistant to erythromycin were even more resistant to rosamicin. | Susceptibility of skin and throat strains of group A streptococci to rosamicin and erythromycin. The minimal inhibitory concentrations of rosamicin and erythromycin were compared for 210 strains of group A streptococcus isolated from a diverse spectrum of streptococcal diseases. Of these strains, 97.6% were inhibited by 0.1 to 0.2 mug of rosamicin per ml, whereas 90.9% were inhibited by 0.025 to 0.05 mug of erythromycin per ml. The minimal inhibitory concentration of rosamicin for 154 strains exceeded that of erythromycin by at least fourfold. Five group A strains of streptococcus that were highly resistant to erythromycin were even more resistant to rosamicin. | [
-0.03718447685241699,
-0.019057437777519226,
-0.03157871961593628,
0.0364999920129776,
0.041423458606004715,
-0.029637623578310013,
-0.008659077808260918,
0.03975091129541397,
-0.06418497115373611,
0.0024250629357993603,
-0.06625046581029892,
-0.028897834941744804,
0.014547201804816723,
0.009627960622310638,
-0.05104390159249306,
-0.02231507934629917,
0.059190090745687485,
-0.006639729253947735,
-0.09359921514987946,
0.03788015618920326,
-0.04657725989818573,
0.022821804508566856,
0.047884635627269745,
-0.07338313013315201,
0.08520154654979706,
-0.01492391899228096,
0.020766275003552437,
-0.00010407197987660766,
-0.06612236052751541,
-0.20527738332748413,
0.0018247197149321437,
-0.03682972863316536,
0.024143293499946594,
0.0018753157928586006,
-0.03211349993944168,
-0.007501694373786449,
-0.003407654818147421,
-0.06067556515336037,
0.0002042665728367865,
0.009526797570288181,
-0.011027641594409943,
0.084208182990551,
0.029636600986123085,
0.0009326498839072883,
-0.013288662768900394,
0.03516503795981407,
-0.06556564569473267,
0.06911111623048782,
0.12232188135385513,
0.021147487685084343,
0.002751831663772464,
0.0039609866216778755,
-0.010766102001070976,
0.08422478288412094,
-0.02078171633183956,
-0.03993042930960655,
0.0056831748224794865,
-0.02667737752199173,
-0.01868571527302265,
0.026125794276595116,
0.013816053047776222,
0.023771921172738075,
-0.12306105345487595,
-0.012607653625309467,
0.006285109557211399,
0.03096982277929783,
0.05394427850842476,
-0.0012478206772357225,
0.03525981679558754,
-0.011574515141546726,
-0.026042601093649864,
0.049897607415914536,
-0.0546133928000927,
0.06485732644796371,
0.015595506876707077,
-0.02439766936004162,
-0.038193751126527786,
-0.027600528672337532,
0.04546677693724632,
0.011716834269464016,
-0.07094109803438187,
0.0033539182040840387,
-0.018943937495350838,
-0.04224802181124687,
0.061835430562496185,
-0.040692877024412155,
0.08596798777580261,
-0.019559305161237717,
0.019886013120412827,
0.0011833857279270887,
0.026400582864880562,
0.011882087215781212,
0.016430404037237167,
-0.03958633914589882,
-0.0797448456287384,
-0.05345359072089195,
0.04260328412055969,
-0.05409780889749527,
0.0070521351881325245,
0.3867318630218506,
-0.024555742740631104,
-0.07395174354314804,
-0.03545020520687103,
0.0002802792878355831,
0.004063745494931936,
-0.003667752258479595,
0.06679011136293411,
0.004591986536979675,
-0.010795575566589832,
0.04339675232768059,
0.020062236115336418,
-0.015193553641438484,
0.05846843868494034,
-0.033431440591812134,
0.017469355836510658,
-0.046946972608566284,
0.03854620084166527,
-0.0478241890668869,
-0.048202723264694214,
0.018957670778036118,
-0.038610927760601044,
-0.05906746909022331,
0.06832902878522873,
-0.021446842700242996,
0.024909479543566704,
0.036045439541339874,
-0.01127577479928732,
0.06942179054021835,
0.07351526618003845,
-0.009926202706992626,
0.014171748422086239,
-0.0156111354008317,
-0.06227997690439224,
-0.01625756546854973,
-0.012007019482553005,
-0.01784576289355755,
0.004301449749618769,
0.014654379338026047,
0.03424132615327835,
-0.03790493682026863,
-0.016967875882983208,
-0.09284225851297379,
0.008100497536361217,
-0.05269130691885948,
-0.026980089023709297,
0.08579778671264648,
0.004898285958915949,
0.014204174280166626,
-0.01909770630300045,
-0.011394685134291649,
0.0066089024767279625,
0.06002919748425484,
-0.009576701559126377,
-0.04525033384561539,
0.005798888858407736,
0.03909039497375488,
-0.017157699912786484,
-0.010638723149895668,
-0.021359065547585487,
0.013404233381152153,
-0.027391616255044937,
-0.013351128436625004,
-0.04595708101987839,
0.0806192010641098,
-0.014365272596478462,
0.005916370544582605,
0.03241089731454849,
-0.006254774518311024,
-0.001088215853087604,
0.014962054789066315,
0.007111165672540665,
0.000515838444698602,
-0.025658002123236656,
-0.011810383759438992,
-0.057160116732120514,
0.04385553300380707,
-0.0007944343378767371,
-0.09546156227588654,
0.057472798973321915,
0.04165421053767204,
0.04409954696893692,
0.05414561182260513,
-0.02382749877870083,
-0.001110442797653377,
0.03346211463212967,
-0.01952073536813259,
-0.05240914598107338,
0.029403477907180786,
0.006254641339182854,
0.04313991963863373,
-0.02058129385113716,
-0.012258823961019516,
-0.050213657319545746,
-0.010942655615508556,
-0.07821262627840042,
-0.004611133132129908,
-0.018614761531352997,
0.010603170841932297,
0.00255981064401567,
0.0010199530515819788,
0.08546395599842072,
0.012901187874376774,
-0.00198957952670753,
0.03532184660434723,
0.055304188281297684,
0.013876005075871944,
0.033460166305303574,
-0.0021023948211222887,
0.0183561984449625,
-0.01346329040825367,
-0.04725072532892227,
0.06461327522993088,
-0.016579164192080498,
0.01784524880349636,
-0.029738137498497963,
0.033953215926885605,
-0.012665366753935814,
0.005007142201066017,
0.018597522750496864,
-0.016599096357822418,
0.08043418824672699,
0.018076220527291298,
-0.08673668652772903,
-0.3178515136241913,
0.045284561812877655,
-0.002394702984020114,
-0.022090844810009003,
0.10338221490383148,
-0.015037581324577332,
0.06547635048627853,
-0.01343570277094841,
-0.025423238053917885,
-0.029935989528894424,
0.0395197719335556,
0.07911084592342377,
-0.023823143914341927,
-0.02831280417740345,
0.030421525239944458,
0.006430263631045818,
0.001350010046735406,
-0.04660376161336899,
0.01925290934741497,
-0.01358635164797306,
0.034180525690317154,
-0.011631792411208153,
0.04699843004345894,
0.014653156511485577,
0.05047185719013214,
-0.0014828921994194388,
0.09343897551298141,
-0.021584995090961456,
-0.014948610216379166,
-0.003955408465117216,
-0.006977972108870745,
0.023191699758172035,
-0.013450000435113907,
-0.019181087613105774,
-0.005819616839289665,
0.01571005955338478,
0.056971896439790726,
-0.014640296809375286,
0.025857267901301384,
-0.013176546432077885,
0.022036796435713768,
0.09531987458467484,
-0.02074812911450863,
-0.035120829939842224,
-0.0508093498647213,
-0.019718782976269722,
0.011210817843675613,
0.07558158785104752,
-0.026489894837141037,
-0.0063568525947630405,
-0.014836184680461884,
0.019473770633339882,
0.0919967070221901,
0.05485610291361809,
-0.005599843338131905,
-0.002940711099654436,
-0.08647258579730988,
0.013981398195028305,
-0.03348769247531891,
-0.013174140825867653,
0.03582589328289032,
-0.01120249554514885,
-0.0067827217280864716,
-0.022021524608135223,
-0.03662532567977905,
-0.0026743884664028883,
-0.018965331837534904,
-0.0431986078619957,
0.10806901752948761,
0.08634474873542786,
-0.01998002827167511,
0.07359755039215088,
-0.02221175655722618,
-0.010875225998461246,
0.03893902525305748,
0.061214178800582886,
-0.012506929226219654,
-0.03836023807525635,
-0.09545519202947617,
0.08766857534646988,
-0.02037203125655651,
-0.04761644825339317,
0.006051770877093077,
-0.0011645021149888635,
-0.013342848978936672,
-0.029183318838477135,
0.006906489375978708,
0.05368218198418617,
0.03353336825966835,
0.01362717617303133,
0.022659577429294586,
-0.05089790001511574,
-0.030311239883303642,
-0.020159060135483742,
0.04602000489830971,
0.026604101061820984,
-0.23111140727996826,
0.009371541440486908,
0.02034221962094307,
0.057589393109083176,
0.005866379011422396,
0.013368535786867142,
0.014728770591318607,
-0.07717001438140869,
-0.04344189912080765,
-0.06201253458857536,
0.06980353593826294,
0.03503314405679703,
0.07452309876680374,
0.060778673738241196,
-0.02215513400733471,
-0.024361666291952133,
0.07021736353635788,
-0.027697807177901268,
0.024572409689426422,
-0.04172920808196068,
0.015724869444966316,
-0.0057191369123756886,
0.09529752284288406,
-0.03347580134868622,
-0.033107247203588486,
0.007632974069565535,
0.007528094109147787,
0.012242292053997517,
-0.06485279649496078,
0.026548346504569054,
-0.04963306710124016,
-0.011442986316978931,
0.03991547226905823,
-0.03406273201107979,
0.08457300066947937,
0.054766662418842316,
-0.021853476762771606,
-0.013377015478909016,
0.02735433168709278,
-0.026092935353517532,
-0.03415948897600174,
-0.017968613654375076,
-0.1022297814488411,
-0.005484242923557758,
0.09501326084136963,
-0.11276142299175262,
-0.01445670984685421,
-0.052210818976163864,
-0.00021331067546270788,
0.0032654560636729,
0.008123630657792091,
0.013581856153905392,
-0.011233309283852577,
-0.03195921704173088,
0.029343679547309875,
-0.02935365028679371,
-0.0016370335360988975,
0.015047847293317318,
-0.02602284401655197,
-0.026655666530132294,
0.019957318902015686,
-0.025649862363934517,
-0.05730244517326355,
0.05386171489953995,
0.0485684759914875
] |
PMID:27146 | The action of lecithin monolayers on mosquitoes I. General observations. | Monolayers of pure egg-lecithin at maximum equilibrium pressure were tested against eight species of mosquito in the laboratory, Anopheline larvae were prevented from surfacing and died in water of low dissolved oxygen content, but culicine larvae survived. The pupae of all species except Culex p. fatigans were killed. Effects on floating eggs, pupal-adult eclosion and adult behaviour are also reported. | The action of lecithin monolayers on mosquitoes I. General observations. Monolayers of pure egg-lecithin at maximum equilibrium pressure were tested against eight species of mosquito in the laboratory, Anopheline larvae were prevented from surfacing and died in water of low dissolved oxygen content, but culicine larvae survived. The pupae of all species except Culex p. fatigans were killed. Effects on floating eggs, pupal-adult eclosion and adult behaviour are also reported. | [
-0.00788867101073265,
-0.040881987661123276,
-0.0016919057816267014,
0.03014563024044037,
-0.009024417027831078,
0.022417983040213585,
0.08928655087947845,
0.09645465016365051,
-0.02228649891912937,
-0.05326265096664429,
-0.004895163234323263,
0.01637144945561886,
0.039780694991350174,
0.05336477607488632,
-0.02355828508734703,
0.0017021296080201864,
-0.027949683368206024,
0.007518426049500704,
-0.07905036956071854,
0.03021637722849846,
0.007186099421232939,
0.0002683017519302666,
0.040842361748218536,
-0.019989097490906715,
-0.005184459500014782,
0.0017855335026979446,
-0.011083338409662247,
-0.013589630834758282,
0.0014153943629935384,
-0.18640942871570587,
-0.0433560386300087,
-0.02581390179693699,
-0.05249112844467163,
-0.06692109256982803,
0.008051549084484577,
0.05081327632069588,
0.032011810690164566,
-0.04730541631579399,
-0.009475324302911758,
-0.00697812344878912,
0.04008304700255394,
0.017543883994221687,
-0.026413798332214355,
-0.006939627230167389,
0.013662694953382015,
0.015878476202487946,
-0.04088443145155907,
0.017256908118724823,
0.09566648304462433,
-0.058321524411439896,
0.007526990491896868,
-0.03674524277448654,
0.007547739893198013,
-0.008929219096899033,
-0.029779478907585144,
-0.06459587067365646,
-0.07716169953346252,
0.010399382561445236,
0.01996064931154251,
0.023937415331602097,
-0.010534042492508888,
-0.01368476077914238,
-0.13517965376377106,
0.11151298135519028,
-0.0035298753064125776,
0.03498508036136627,
-0.01951669342815876,
0.018331874161958694,
0.03731590881943703,
0.03928685933351517,
-0.024764468893408775,
0.04172850772738457,
0.001669716089963913,
-0.07112128287553787,
0.001999632688239217,
0.05667966231703758,
0.008863475173711777,
-0.06326273083686829,
-0.026592401787638664,
-0.002403452293947339,
-0.0029145602602511644,
-0.04922523349523544,
0.04383767768740654,
-0.005406698677688837,
0.05611881613731384,
0.03435077890753746,
0.04333195090293884,
-0.009722253307700157,
0.05975984409451485,
-0.037939492613077164,
0.02177579142153263,
-0.02373182773590088,
0.006771707907319069,
-0.005162591114640236,
-0.03861343488097191,
0.04405795410275459,
-0.032492198050022125,
-0.05679894611239433,
-0.033713627606630325,
0.4105854332447052,
-0.05675482749938965,
-0.018268391489982605,
-0.04635739326477051,
-0.012420103885233402,
0.011895221658051014,
0.017032857984304428,
-0.022313686087727547,
-0.04985347390174866,
0.012220500037074089,
0.09224396198987961,
0.04051629826426506,
0.029502246528863907,
0.0162362027913332,
0.012670934200286865,
-0.0226726233959198,
-0.015257722698152065,
0.03364458307623863,
-0.04825098440051079,
-0.04119400307536125,
-0.02087518572807312,
-0.026204261928796768,
-0.024495907127857208,
0.0492134764790535,
0.04401068389415741,
0.04878809675574303,
0.04602743685245514,
0.02018705941736698,
0.06877429783344269,
0.02706831321120262,
0.008360560983419418,
0.07445051521062851,
-0.0023618959821760654,
-0.012788940221071243,
-0.025702692568302155,
-0.01358261238783598,
-0.0073091150261461735,
0.010989817790687084,
-0.004190086852759123,
-0.01866995356976986,
-0.02119126170873642,
-0.05353529378771782,
-0.042666468769311905,
-0.028662700206041336,
-0.12365442514419556,
-0.0001840716286096722,
0.09226933121681213,
0.07193714380264282,
0.03544095158576965,
-0.0373138003051281,
0.03904939442873001,
-0.02353728376328945,
0.026669075712561607,
-0.02290099300444126,
0.02748965285718441,
0.006828670389950275,
0.023941390216350555,
-0.026860473677515984,
0.029276732355356216,
-0.04551253095269203,
-0.023972071707248688,
-0.058272287249565125,
0.05318684130907059,
-0.026113225147128105,
0.09503538906574249,
-0.026296615600585938,
-0.04350799694657326,
-0.008253355510532856,
0.05372529476881027,
0.03287820890545845,
0.012726419605314732,
0.00972306914627552,
-0.036779481917619705,
0.027146009728312492,
-0.01878589764237404,
-0.05350126698613167,
0.044225458055734634,
-0.040768589824438095,
-0.03001621551811695,
0.03418675810098648,
-0.0192412156611681,
0.012618647888302803,
-0.03810424357652664,
-0.008470027707517147,
0.056474748998880386,
-0.008268904872238636,
0.0003645053948275745,
-0.05909880995750427,
0.009351617656648159,
0.022653033956885338,
-0.011514588259160519,
-0.087738536298275,
-0.08757343143224716,
-0.020256588235497475,
0.013612714596092701,
-0.009692137129604816,
-0.008200062438845634,
-0.023554252460598946,
0.016714952886104584,
0.05576138198375702,
-0.05810598284006119,
0.06336560100317001,
-0.020918281748890877,
-0.005716987885534763,
0.04240725189447403,
0.026486070826649666,
0.08958907425403595,
0.020347297191619873,
0.007361029274761677,
0.0591682493686676,
-0.005275586619973183,
-0.02934102900326252,
-0.02389710023999214,
-0.0008183274767361581,
-0.05663781985640526,
0.00795643962919712,
0.07564543187618256,
0.010998726822435856,
0.02020997554063797,
0.07677777856588364,
-0.041422173380851746,
0.05519409850239754,
-0.0670812800526619,
-0.029768189415335655,
-0.3045385479927063,
-0.06962805986404419,
-0.022463146597146988,
-0.0677100419998169,
0.10308706760406494,
-0.003192574018612504,
0.04125991463661194,
-0.036904383450746536,
-0.0012007256736978889,
-0.08571340143680573,
0.02726169303059578,
0.032620869576931,
-0.05938059464097023,
-0.009169270284473896,
0.03599764406681061,
0.06529360264539719,
0.028427239507436752,
-0.005618080031126738,
0.024285512045025826,
0.021801887080073357,
0.04872030392289162,
-0.029106080532073975,
0.0015376583905890584,
0.00037214887561276555,
0.06817294657230377,
-0.023132065311074257,
0.1479375660419464,
0.015253446064889431,
0.03666534274816513,
-0.011945714242756367,
-0.0034737035166472197,
-0.0027102783787995577,
-0.016898175701498985,
-0.07448586821556091,
-0.021884415298700333,
0.01986503042280674,
-0.006882834248244762,
-0.07729633897542953,
-0.01664349064230919,
-0.0511411614716053,
-0.02880292758345604,
0.014706214889883995,
0.043477825820446014,
-0.04841399937868118,
-0.03060438297688961,
-0.06313600391149521,
0.041888121515512466,
0.030140772461891174,
-0.038741398602724075,
0.03442172706127167,
0.0021449413616210222,
0.03749392554163933,
0.06506512314081192,
0.05438101291656494,
-0.0016098706983029842,
0.014725477434694767,
-0.032837722450494766,
-0.013970904983580112,
-0.07527677714824677,
0.04785732179880142,
-0.06412067264318466,
0.019942492246627808,
0.02435980923473835,
-0.02082008868455887,
0.004732947796583176,
-0.020906459540128708,
0.07871823012828827,
-0.027732256799936295,
0.06822297722101212,
0.0018878561677411199,
0.013777149841189384,
0.1044594943523407,
-0.05010037496685982,
-0.008410472422838211,
0.02591262012720108,
0.019829807803034782,
0.013428976759314537,
-0.05146612972021103,
-0.0240203607827425,
0.027364885434508324,
-0.021228669211268425,
0.0008986237226054072,
0.0019393701804801822,
-0.011896347627043724,
0.010527553968131542,
0.045465558767318726,
-0.025803910568356514,
-0.011542931199073792,
0.041049178689718246,
-0.009781579487025738,
-0.01735694520175457,
-0.018163178116083145,
-0.02900066040456295,
-0.0020145236048847437,
0.0009770721662789583,
0.06203712895512581,
-0.24602210521697998,
0.011617007665336132,
0.017841409891843796,
0.015271988697350025,
-0.020380815491080284,
0.057813093066215515,
0.034217268228530884,
-0.033596839755773544,
-0.03736429661512375,
0.061430107802152634,
0.017816010862588882,
0.0022919203620404005,
0.05542192608118057,
0.061374831944704056,
0.013100435957312584,
0.02917315810918808,
-0.009732650592923164,
-0.06085921451449394,
0.0018661310896277428,
0.016076382249593735,
-0.061146803200244904,
-0.011624819599092007,
0.1361137181520462,
0.01321958564221859,
-0.059748608618974686,
0.03887234628200531,
-0.014712156727910042,
0.08815352618694305,
-0.060403283685445786,
0.011907229200005531,
-0.022487863898277283,
-0.011938132345676422,
0.03586235269904137,
-0.07686468958854675,
0.021980436518788338,
0.04787425696849823,
-0.03710851073265076,
-0.03702880069613457,
0.008329996839165688,
0.004123862367123365,
0.00865614041686058,
-0.02628049999475479,
-0.004842774011194706,
0.028379905968904495,
0.025318335741758347,
0.013898948207497597,
0.01408228650689125,
-0.00333554414100945,
-0.05314775928854942,
-0.017007486894726753,
-0.04429095610976219,
-0.01901635341346264,
0.02592804655432701,
-0.006910880096256733,
0.01565050706267357,
-0.010351939126849174,
-0.018022505566477776,
0.003708259202539921,
-0.031221460551023483,
0.03584987670183182,
0.04086337983608246,
-0.023433202877640724,
-0.04561369866132736,
0.043023139238357544,
0.05301867425441742
] |
PMID:27147 | The action of lecithin monolayers on mosquitoes. II. Action on the respiratory structures. | Experiments on the interaction between the water surface and the respiratory structures of larvae and pupae of five species of mosquitoes showed that in the presence of a lecithin monolayer, a stable, soap-bubble type film is formed which prevents the insect from surfacing. Subsequently, wetting of the hydrophobic areas takes place. In pupae, buoyancy is lost through flooding of the ventral air space. | The action of lecithin monolayers on mosquitoes. II. Action on the respiratory structures. Experiments on the interaction between the water surface and the respiratory structures of larvae and pupae of five species of mosquitoes showed that in the presence of a lecithin monolayer, a stable, soap-bubble type film is formed which prevents the insect from surfacing. Subsequently, wetting of the hydrophobic areas takes place. In pupae, buoyancy is lost through flooding of the ventral air space. | [
-0.053750816732645035,
-0.025670869275927544,
-0.0034458006266504526,
0.03556771203875542,
0.0030789426527917385,
-0.015171962790191174,
0.09373199194669724,
0.04224064201116562,
-0.020625632256269455,
-0.008948453702032566,
-0.049411334097385406,
0.016490675508975983,
0.017665736377239227,
0.054624855518341064,
0.020427348092198372,
-0.0108767906203866,
-0.022582625970244408,
0.05036471039056778,
-0.06923046708106995,
0.04598931223154068,
0.022172685712575912,
-0.005275933537632227,
0.0606192983686924,
-0.009146696887910366,
-0.014848250895738602,
-0.019743207842111588,
-0.004792825784534216,
0.011856593191623688,
0.0015531398821622133,
-0.19737575948238373,
-0.02711428329348564,
-0.022794578224420547,
-0.033701129257678986,
-0.03700678050518036,
0.024600530043244362,
0.03592131286859512,
0.014250420033931732,
-0.05703681707382202,
-0.04508141800761223,
0.021050438284873962,
0.013502249494194984,
0.022469647228717804,
-0.020320825278759003,
-0.02827472984790802,
0.008387807756662369,
-0.021582644432783127,
-0.038749899715185165,
0.02263900451362133,
0.06908466666936874,
-0.07863226532936096,
0.041865862905979156,
-0.055397871881723404,
0.024224283173680305,
-0.009842602536082268,
-0.05093858018517494,
-0.05251913145184517,
-0.04544684663414955,
0.015088468790054321,
0.010563479736447334,
0.015846997499465942,
0.006063493434339762,
0.009443972259759903,
-0.1260456144809723,
0.14659357070922852,
0.015484911389648914,
0.02768787182867527,
-0.019690465182065964,
0.015249271877110004,
0.039272502064704895,
0.018413925543427467,
-0.02772405557334423,
0.05706039443612099,
0.004359373822808266,
-0.04407322034239769,
0.03612954914569855,
0.03750978410243988,
-0.03504065424203873,
-0.06357668340206146,
-0.0230153426527977,
0.03553733229637146,
0.009748117998242378,
-0.04150542616844177,
0.02527494728565216,
-0.014284833334386349,
0.048376962542533875,
0.04066355526447296,
0.06030240282416344,
-0.059636618942022324,
0.025187961757183075,
-0.024141361936926842,
-0.002568298950791359,
-0.04828338325023651,
-0.047688763588666916,
0.04964997619390488,
-0.059261828660964966,
0.03839791938662529,
-0.022298840805888176,
-0.035256411880254745,
-0.007802242878824472,
0.39596685767173767,
-0.07530348747968674,
0.025240443646907806,
0.0135668208822608,
-0.006395178381353617,
-0.014367440715432167,
0.04128054156899452,
-0.059238776564598083,
-0.046977683901786804,
-0.018689675256609917,
0.05929683893918991,
0.03926030546426773,
0.040919993072748184,
0.0008170848013833165,
-0.010992267169058323,
-0.026668578386306763,
-0.03213810920715332,
0.0332983061671257,
-0.00967155210673809,
-0.0455467514693737,
-0.061949722468853,
0.01984071359038353,
-0.02069326676428318,
0.03878549486398697,
0.013716221787035465,
0.012156220152974129,
0.02678748406469822,
-0.015950817614793777,
0.08448659628629684,
0.055145230144262314,
-0.008375228382647038,
0.07873466610908508,
-0.007768752984702587,
0.004272563848644495,
-0.050194814801216125,
-0.018045151606202126,
-0.028504647314548492,
0.014386787079274654,
-0.033607419580221176,
-0.03704706206917763,
-0.006851118989288807,
-0.04957994073629379,
-0.05071374028921127,
0.02057359553873539,
-0.09325180947780609,
-0.017274826765060425,
0.078013576567173,
0.08560416102409363,
0.02020442858338356,
-0.02799404039978981,
0.04058259725570679,
-0.039987582713365555,
0.0231039896607399,
-0.021428842097520828,
0.030572857707738876,
0.010528294369578362,
-0.01224465761333704,
-0.04921017959713936,
0.040246520191431046,
-0.024062978103756905,
-0.07124434411525726,
-0.06786688417196274,
0.06577659398317337,
-0.022326979786157608,
0.10830150544643402,
-0.010849989950656891,
-0.034792136400938034,
0.005829190835356712,
0.039100807160139084,
0.03800229728221893,
0.052649181336164474,
-0.0006824178854003549,
-0.0022879757452756166,
0.02730080671608448,
-0.04455755278468132,
-0.04416492581367493,
0.06053096801042557,
-0.03841886669397354,
0.0007165997521951795,
0.06515587866306305,
0.009761426597833633,
0.02362966537475586,
-0.050854552537202835,
-0.0470249205827713,
0.03097609616816044,
0.012971659190952778,
-0.01881726272404194,
-0.06382165104150772,
0.01502871885895729,
0.046216931194067,
-0.012768796645104885,
-0.07079972326755524,
-0.07076374441385269,
0.005556827876716852,
-0.014214614406228065,
0.01316917035728693,
-0.034534960985183716,
-0.00975294504314661,
-0.009190003387629986,
0.03421564772725105,
-0.01348904985934496,
0.0513952262699604,
0.04485437273979187,
0.004945122171193361,
0.039416659623384476,
0.004872634541243315,
0.08805100619792938,
0.044887345284223557,
0.010117878206074238,
0.04274381324648857,
-0.00586030213162303,
-0.04277205467224121,
-0.016193779185414314,
-0.02253771387040615,
-0.05936376377940178,
0.014383956789970398,
0.06795994192361832,
0.012023085728287697,
-0.02806301787495613,
0.06431963294744492,
-0.036333054304122925,
0.04212261363863945,
-0.07572910934686661,
-0.020790057256817818,
-0.2940765917301178,
-0.05993208661675453,
-0.028513608500361443,
-0.05860154330730438,
0.10086872428655624,
-0.016246570274233818,
0.0606679767370224,
-0.02704211324453354,
0.016157116740942,
-0.10178463906049728,
0.04994433745741844,
-0.008667528629302979,
-0.04739627242088318,
-0.019204994663596153,
0.0001850418047979474,
0.043177101761102676,
0.030575750395655632,
-0.0146649070084095,
0.04218262434005737,
0.03187844157218933,
0.06058402359485626,
-0.03142080083489418,
0.03808584064245224,
0.015431266278028488,
0.08671185374259949,
-0.018448369577527046,
0.1538345068693161,
0.012882458977401257,
0.024593845009803772,
0.025372857227921486,
0.048736464232206345,
0.005920193158090115,
-0.020174579694867134,
-0.05645016208291054,
-0.019808953627943993,
0.026195993646979332,
0.02670889161527157,
-0.07326951622962952,
-0.002254143822938204,
-0.057351499795913696,
-0.039171215146780014,
0.059651147574186325,
0.01261679083108902,
-0.018567046150565147,
-0.03591807559132576,
-0.06686106324195862,
0.007520354352891445,
0.018390441313385963,
-0.04416068643331528,
-0.02802899479866028,
0.028842497617006302,
0.009634740650653839,
0.03526851907372475,
0.04272409528493881,
0.008880049921572208,
0.0069711776450276375,
0.018573641777038574,
-0.050198208540678024,
-0.0714799016714096,
0.04083842784166336,
-0.054560378193855286,
0.042598892003297806,
0.04697132110595703,
-0.005849096458405256,
0.007358085364103317,
0.005137926898896694,
0.04924418777227402,
-0.02094636671245098,
0.06303757429122925,
-0.006743141449987888,
0.010661525651812553,
0.09924043715000153,
-0.038727469742298126,
0.006048331502825022,
0.011653480120003223,
-0.006443493068218231,
0.020204953849315643,
-0.056406278163194656,
-0.046677421778440475,
0.0016730849165469408,
-0.012390224263072014,
-0.015704967081546783,
0.02329452894628048,
-0.02557172067463398,
0.006067190319299698,
0.037950966507196426,
0.002077449345961213,
-0.042730771005153656,
-0.004948331508785486,
-0.038438111543655396,
-0.02177143469452858,
0.0086713507771492,
-0.022777223959565163,
-0.03433317691087723,
-0.0021756107453256845,
0.027851244434714317,
-0.2644488513469696,
0.018203135579824448,
0.020474573597311974,
0.04641791433095932,
-0.027084125205874443,
0.03931507468223572,
0.04017999395728111,
-0.04777112975716591,
0.014077731408178806,
0.06941839307546616,
-0.02456265315413475,
0.010252431966364384,
0.023884469643235207,
0.06804383546113968,
0.009299998171627522,
0.034758999943733215,
0.03717407211661339,
-0.06813599169254303,
0.00171720574144274,
-0.009793396107852459,
-0.04965654015541077,
-0.04661821573972702,
0.14271065592765808,
-0.01597530208528042,
-0.049773868173360825,
0.003518239129334688,
-0.0103524224832654,
0.06287874281406403,
-0.07417948544025421,
0.011253098957240582,
-0.005627165548503399,
0.004241736140102148,
0.0691266655921936,
-0.03023379109799862,
-0.02185259759426117,
0.04701460897922516,
-0.009457915090024471,
-0.0004052800650242716,
0.011814156547188759,
-0.007712542079389095,
0.01708744280040264,
-0.02617470547556877,
-0.002497125184163451,
-0.0017391768051311374,
0.02352238819003105,
-0.005637406837195158,
0.03703758120536804,
0.030259696766734123,
-0.007051653694361448,
0.03250380605459213,
-0.04467989504337311,
-0.022971700876951218,
0.015501828864216805,
-0.01590595208108425,
0.028772953897714615,
0.009636503644287586,
-0.03438077121973038,
-0.015482082962989807,
-0.019091781228780746,
0.053070034831762314,
0.04067084193229675,
-0.054614655673503876,
-0.06508372724056244,
0.05375897139310837,
0.05137067660689354
] |
PMID:27151 | Hypotensive action of beta-blocking drugs injected into the cerebral ventricle of the rat. | Pindolol, propranolol, practolol, N-isopropyl-p-nitrophenyl-ethanolamine (INPEA), d and l-alprenolol were injected into the lateral cerebral ventricle of the anaesthetized rat. Except for INPEA, which has no action at the doses used, these various beta-blocking agents produce a dose-related fall in blood pressure. D-alprenolol and practolol seem to be the least active of the hypotensive agents. Our results show some relationship between the beta-blocking potency of the drugs studied and the hypotension induced when they were injected in the lateral ventricle. | Hypotensive action of beta-blocking drugs injected into the cerebral ventricle of the rat. Pindolol, propranolol, practolol, N-isopropyl-p-nitrophenyl-ethanolamine (INPEA), d and l-alprenolol were injected into the lateral cerebral ventricle of the anaesthetized rat. Except for INPEA, which has no action at the doses used, these various beta-blocking agents produce a dose-related fall in blood pressure. D-alprenolol and practolol seem to be the least active of the hypotensive agents. Our results show some relationship between the beta-blocking potency of the drugs studied and the hypotension induced when they were injected in the lateral ventricle. | [
-0.04971393942832947,
0.021158350631594658,
0.004588908515870571,
-0.04802679643034935,
-0.007203518878668547,
0.048276592046022415,
0.028790026903152466,
0.008601906709372997,
0.007577683310955763,
-0.044983766973018646,
-0.02077568508684635,
-0.013379617594182491,
-0.015472077764570713,
0.03616049885749817,
-0.07092985510826111,
-0.014530020765960217,
0.0013290147762745619,
-0.015051501803100109,
-0.032587967813014984,
0.009751325473189354,
0.0075297425501048565,
-0.0016683368012309074,
0.04113991931080818,
0.02141379565000534,
-0.026453368365764618,
-0.007308232132345438,
-0.006013816688209772,
0.01914621889591217,
-0.056471534073352814,
-0.18670053780078888,
0.03407533839344978,
0.0031290450133383274,
-0.0008161796140484512,
-0.053640928119421005,
-0.028029251843690872,
0.006635323166847229,
-0.012351425364613533,
0.034985192120075226,
-0.010531118139624596,
0.038207046687603,
0.04149772599339485,
-0.03445250168442726,
-0.007587791886180639,
-0.009711428545415401,
-0.0039744810201227665,
-0.031075498089194298,
-0.003700641915202141,
-0.03701037913560867,
0.1060618907213211,
-0.005251643713563681,
0.024896224960684776,
0.00845058262348175,
-0.012523186393082142,
0.02040795423090458,
-0.02039155922830105,
-0.06620925664901733,
-0.03780793398618698,
0.0021293896716088057,
0.0227497685700655,
0.09419094771146774,
-0.012587379664182663,
0.01656166836619377,
-0.15476979315280914,
0.04028443619608879,
0.02416493371129036,
0.07868262380361557,
-0.04846826195716858,
0.052069637924432755,
0.05805867165327072,
0.03796401247382164,
0.045221131294965744,
0.008513275533914566,
0.031329426914453506,
-0.013868376612663269,
-0.00729734729975462,
-0.02183466963469982,
0.019111240282654762,
-0.0032625896856188774,
-0.0021016818936914206,
-0.029395397752523422,
0.058949973434209824,
-0.02622140385210514,
-0.03498586267232895,
-0.00739095127210021,
0.017530769109725952,
-0.05028838291764259,
0.07530062645673752,
-0.05851302668452263,
-0.006051318719983101,
-0.014110562391579151,
-0.06905937939882278,
-0.003668898018077016,
-0.03450193256139755,
-0.001303700846619904,
-0.03479284420609474,
0.007189773488789797,
-0.029269792139530182,
-0.02139289304614067,
-0.029070895165205002,
0.38572773337364197,
0.019495178014039993,
0.05506129562854767,
-0.05142846331000328,
-0.007805326487869024,
0.018550019711256027,
-0.053269997239112854,
0.024092333391308784,
-0.018132122233510017,
-0.02469080127775669,
0.04995573312044144,
0.07320082932710648,
0.005015457980334759,
0.08216962218284607,
-0.037689901888370514,
-0.014383591711521149,
-0.10110624879598618,
0.06183670833706856,
-0.02695831097662449,
-0.021699419245123863,
0.025733720511198044,
-0.0681963860988617,
-0.015658069401979446,
0.011984813958406448,
0.04711536690592766,
0.0384543240070343,
-0.03629658743739128,
-0.012254364788532257,
0.08782973885536194,
0.07079631090164185,
0.030145268887281418,
0.03973972797393799,
0.03847971558570862,
0.012673113495111465,
0.03434586152434349,
0.013502135872840881,
-0.01846281997859478,
0.021816682070493698,
-0.011540785431861877,
0.032148443162441254,
-0.06421703100204468,
0.0023678597062826157,
-0.011932055465877056,
0.05041977018117905,
-0.06359197199344635,
-0.037431951612234116,
0.07459951192140579,
0.02584216184914112,
-0.007235608994960785,
-0.05208355188369751,
0.06153353303670883,
-0.09043262153863907,
0.0328739732503891,
0.005996222607791424,
0.0966184139251709,
-0.04592083767056465,
-0.021824361756443977,
0.014418147504329681,
0.03755804896354675,
-0.03126577287912369,
-0.03305698558688164,
-0.06594245880842209,
-0.05241473391652107,
-0.029732024297118187,
0.02486994117498398,
-0.008947738446295261,
0.06403840333223343,
-0.04477016255259514,
-0.010804026387631893,
0.0034020410384982824,
0.024833036586642265,
-0.010117008350789547,
0.013525730930268764,
0.020248061046004295,
-0.027353700250387192,
-0.04997815191745758,
0.06281419098377228,
0.04368362948298454,
-0.039411064237356186,
0.05694204196333885,
-0.06929367780685425,
0.01732041873037815,
-0.00964215025305748,
-0.020000195130705833,
-0.008846334181725979,
-0.0013653719797730446,
0.06807830929756165,
-0.03704777732491493,
-0.0010829921811819077,
-0.00554347550496459,
0.05810751020908356,
-0.0383346863090992,
-0.0338134728372097,
-0.09562259912490845,
0.002699536271393299,
-0.032857902348041534,
-0.02815779112279415,
-0.05071093887090683,
0.024599697440862656,
-0.040825821459293365,
-0.019229965284466743,
0.06246272474527359,
-0.0018523142207413912,
-0.00803485419601202,
0.0437430776655674,
0.030576182529330254,
0.0704481303691864,
-0.010040147230029106,
-0.00040599619387649,
0.06975620239973068,
-0.00006752044282620773,
-0.01794213429093361,
0.030363628640770912,
0.04736689478158951,
0.027218379080295563,
-0.04079163447022438,
0.04824661836028099,
-0.023785391822457314,
-0.0015313895419239998,
0.010076386854052544,
0.0227095577865839,
0.03564672917127609,
-0.056680262088775635,
0.02303992584347725,
-0.3166717290878296,
-0.062153160572052,
-0.031223079189658165,
-0.05595165491104126,
0.029086463153362274,
-0.056063245981931686,
0.044609248638153076,
-0.050187088549137115,
-0.053101737052202225,
-0.002786035183817148,
0.03574993461370468,
0.029000641778111458,
-0.01093589048832655,
0.004663045983761549,
-0.02493632584810257,
0.03677961230278015,
0.04582974314689636,
-0.037624046206474304,
0.015588200651109219,
0.014833486638963223,
0.011164402589201927,
-0.04982982203364372,
-0.044197045266628265,
0.025230616331100464,
0.05066373571753502,
0.012961449101567268,
0.08647739887237549,
-0.00806356780230999,
-0.010753070004284382,
-0.032049648463726044,
-0.01042220089584589,
0.048443615436553955,
-0.004226937424391508,
-0.10130123049020767,
0.034761153161525726,
-0.047502074390649796,
0.07243415713310242,
0.04144168272614479,
0.02449127472937107,
0.012273445725440979,
-0.03946053981781006,
0.034448571503162384,
0.023565512150526047,
-0.003039092291146517,
-0.045883066952228546,
0.01818200759589672,
-0.0049844891764223576,
0.055761683732271194,
-0.03696160018444061,
0.0038916170597076416,
-0.016123585402965546,
-0.01610695943236351,
0.0069496347568929195,
0.03576882928609848,
0.06879661232233047,
-0.009583798237144947,
-0.06449630856513977,
-0.04315656051039696,
-0.025273684412240982,
-0.020537182688713074,
0.023129696026444435,
-0.005564976017922163,
-0.022576263174414635,
-0.041969817131757736,
-0.020202040672302246,
0.014818004332482815,
0.028865303844213486,
0.027747616171836853,
0.04231296479701996,
0.05274394527077675,
-0.0012503775069490075,
0.13403308391571045,
-0.026660898700356483,
0.0032289139926433563,
0.04736890271306038,
-0.010710230097174644,
-0.04009605199098587,
-0.04477475583553314,
-0.03206333890557289,
0.025187749415636063,
-0.007041736971586943,
-0.04894918575882912,
0.01649279147386551,
0.02664163149893284,
-0.053259313106536865,
-0.03593834117054939,
0.016268771141767502,
0.006507087964564562,
0.09284660220146179,
-0.02082536183297634,
-0.041713640093803406,
0.026675229892134666,
-0.0017966256709769368,
-0.026328543201088905,
0.04402250051498413,
-0.013499517925083637,
-0.2705087959766388,
0.0017329002730548382,
0.06994353979825974,
-0.0019351558294147253,
0.012924046255648136,
0.008222437463700771,
0.052441228181123734,
-0.04541539028286934,
-0.030446723103523254,
0.018174538388848305,
-0.05998895689845085,
0.0703093484044075,
0.06217305362224579,
0.056736432015895844,
-0.03734453022480011,
0.005454905331134796,
0.021199624985456467,
-0.015030110254883766,
0.07299470156431198,
-0.06158727779984474,
-0.004045723006129265,
0.0002157304115826264,
0.13261678814888,
0.03159743547439575,
-0.017767805606126785,
0.04268914461135864,
0.026400644332170486,
0.03322127088904381,
-0.031954310834407806,
0.06646715104579926,
-0.024225354194641113,
0.011020979844033718,
0.07907480746507645,
-0.060071900486946106,
0.050492845475673676,
0.03270627558231354,
-0.10054608434438705,
0.01128254272043705,
0.03682940453290939,
-0.0013838181039318442,
-0.035166412591934204,
0.04392538592219353,
-0.07139917463064194,
0.03860839456319809,
0.0479448139667511,
-0.07726326584815979,
-0.02649269811809063,
-0.017681317403912544,
-0.01540335826575756,
0.04176637902855873,
-0.035004470497369766,
-0.019737068563699722,
0.034184530377388,
0.0047534070909023285,
-0.0213421992957592,
-0.04421846568584442,
-0.004242900293320417,
-0.007447359152138233,
0.0030735363252460957,
-0.032824188470840454,
0.015065541490912437,
-0.041046321392059326,
-0.010567385703325272,
0.0894954651594162,
0.052882008254528046
] |
PMID:27152 | Ganglion blocking effects of streptomycin. | The effects of streptomycin on the postganglionic compound action potential, presynaptic nerve terminal spike and preganglionic nerve action potnetial were studied in rabbit and rat superior cervical gnaglia. Streptomycin had a dose-dependent, blocking effect on the postganglionic action potential. The drug was found to have a preferential blocking effect on the first spike, S1, of the compound postganglionic action potential, but the second, S2, major spike was depressed at higher concentrations. At 1 mg/ml, streptomycin reduced the amplitude of the presynaptic nerve terminal action potential, but had no significant effect on the action potential recorded from the cervical sympathetic preganglionic nerve. Increasing the concentrations of Ca++ in the bathing solution antagonized the blocking effect of the antibiotic on the postganglionic action potential. The results are interpreted as indicating that ganglionic blockade by streptomycin is due, mainly, to a presynaptic effect which results in a decreased release of acetylcholine. | Ganglion blocking effects of streptomycin. The effects of streptomycin on the postganglionic compound action potential, presynaptic nerve terminal spike and preganglionic nerve action potnetial were studied in rabbit and rat superior cervical gnaglia. Streptomycin had a dose-dependent, blocking effect on the postganglionic action potential. The drug was found to have a preferential blocking effect on the first spike, S1, of the compound postganglionic action potential, but the second, S2, major spike was depressed at higher concentrations. At 1 mg/ml, streptomycin reduced the amplitude of the presynaptic nerve terminal action potential, but had no significant effect on the action potential recorded from the cervical sympathetic preganglionic nerve. Increasing the concentrations of Ca++ in the bathing solution antagonized the blocking effect of the antibiotic on the postganglionic action potential. The results are interpreted as indicating that ganglionic blockade by streptomycin is due, mainly, to a presynaptic effect which results in a decreased release of acetylcholine. | [
-0.013334335759282112,
-0.023634962737560272,
0.02465052343904972,
-0.008731946349143982,
-0.0077689108438789845,
-0.0042185536585748196,
0.001937133027240634,
0.009393899701535702,
0.00930944737046957,
-0.037194907665252686,
0.0015738217625766993,
0.04311042279005051,
0.03522522374987602,
0.04948481544852257,
-0.06276638060808182,
-0.018668022006750107,
0.012230864726006985,
-0.03602781891822815,
-0.042908500880002975,
-0.03960135579109192,
-0.07570500671863556,
0.04116512089967728,
0.020080748945474625,
-0.035690464079380035,
0.042913030833005905,
-0.04311283305287361,
-0.014915363863110542,
0.018561262637376785,
-0.03976608067750931,
-0.201556995511055,
0.05308261513710022,
-0.0028016280848532915,
-0.047108057886362076,
-0.08078932017087936,
-0.018513578921556473,
0.003117574844509363,
-0.056258782744407654,
0.0252375490963459,
-0.05376357585191727,
-0.0030862062703818083,
0.03678024932742119,
0.04100518673658371,
0.025110675022006035,
0.02589232474565506,
-0.009394329972565174,
-0.017742853611707687,
-0.0008885641000233591,
-0.019977256655693054,
0.09721411764621735,
0.011961951851844788,
0.01520076859742403,
-0.02094770036637783,
-0.013658707961440086,
0.039075516164302826,
0.006582432426512241,
-0.04146838188171387,
-0.039420709013938904,
0.07368528097867966,
-0.013434551656246185,
0.026597818359732628,
0.015591048635542393,
0.026446957141160965,
-0.14255326986312866,
0.012676673009991646,
0.039968594908714294,
0.035895414650440216,
0.04675203189253807,
0.013279136270284653,
0.04633428156375885,
0.08295776695013046,
-0.062350064516067505,
0.08123639225959778,
-0.0050159902311861515,
0.01353922113776207,
0.00569164240732789,
-0.0010050704004243016,
-0.02666994370520115,
0.024433905258774757,
0.04417192190885544,
0.03898479789495468,
-0.02097230777144432,
0.0046105580404400826,
-0.027576083317399025,
-0.0655185729265213,
0.006577251013368368,
-0.015592600218951702,
0.05499522015452385,
-0.018702290952205658,
-0.012919962406158447,
0.02574566751718521,
-0.045709289610385895,
0.00023393724404741079,
-0.035733308643102646,
-0.059985432773828506,
-0.06436675041913986,
0.03462338447570801,
-0.012015052139759064,
-0.02797788567841053,
-0.05065838247537613,
0.3190418779850006,
0.014524799771606922,
-0.03290336951613426,
-0.05057026445865631,
0.028409508988261223,
-0.027276255190372467,
-0.002904733410105109,
0.021604925394058228,
0.02491958998143673,
0.013111953623592854,
0.04802998527884483,
0.11398095637559891,
-0.021338462829589844,
0.06668607890605927,
0.034292250871658325,
-0.019582631066441536,
0.014384732581675053,
0.06276588886976242,
0.03754064813256264,
0.019272686913609505,
-0.04621720686554909,
-0.03806600347161293,
0.00958526972681284,
0.02965889684855938,
-0.007412455044686794,
0.0403779037296772,
0.02756151556968689,
-0.056519538164138794,
0.07132319360971451,
0.03636980801820755,
0.056400116533041,
0.06264019012451172,
-0.026483291760087013,
0.018279574811458588,
0.03537736088037491,
0.04897436127066612,
-0.006887374445796013,
0.02607223391532898,
-0.044446881860494614,
0.012853818945586681,
-0.08691342920064926,
-0.019843393936753273,
-0.055896464735269547,
-0.018269529566168785,
-0.06428791582584381,
-0.051851559430360794,
0.11303301900625229,
0.02696860209107399,
-0.029391400516033173,
-0.05353306606411934,
0.05964333564043045,
0.027998967096209526,
0.037949834018945694,
-0.00015552838158328086,
0.006253386382013559,
-0.019057856872677803,
0.0953303724527359,
-0.02760232426226139,
0.03306497633457184,
-0.037043552845716476,
-0.00681109307333827,
-0.1038520559668541,
-0.037872038781642914,
-0.026321494951844215,
0.038703612983226776,
0.029880886897444725,
0.033748988062143326,
0.00015964292106218636,
-0.02606300078332424,
-0.036002639681100845,
0.04401378706097603,
-0.026057235896587372,
0.037981484085321426,
-0.039132051169872284,
-0.08008335530757904,
-0.12054144591093063,
0.046926114708185196,
-0.009265783242881298,
-0.06427143514156342,
0.03192761167883873,
0.005728671792894602,
0.020736834034323692,
0.03805939853191376,
0.026230303570628166,
-0.024217205122113228,
0.0020276932045817375,
0.03850199282169342,
-0.07135284692049026,
0.059427663683891296,
0.0073021589778363705,
-0.00873746257275343,
-0.013328910805284977,
-0.021557552739977837,
-0.08502866327762604,
-0.000415297516155988,
-0.0807962715625763,
-0.03548874706029892,
-0.034134507179260254,
-0.008953198790550232,
-0.01980755478143692,
-0.04351269453763962,
0.0735556110739708,
0.03240007907152176,
-0.06484828144311905,
0.0573006346821785,
0.030560104176402092,
0.017333175987005234,
-0.040774866938591,
-0.03400500491261482,
-0.013217536732554436,
0.007938914000988007,
-0.01162880938500166,
0.018936756998300552,
0.07455019652843475,
-0.007552004419267178,
-0.04706656187772751,
0.037118714302778244,
-0.042357780039310455,
-0.008675025776028633,
0.032024551182985306,
0.03633562847971916,
0.03517644479870796,
-0.006142369471490383,
-0.01799877919256687,
-0.3335324227809906,
-0.015375371091067791,
0.023311929777264595,
-0.003126921132206917,
0.04289845749735832,
-0.03673464059829712,
0.020507311448454857,
-0.009209226816892624,
-0.02643931470811367,
-0.08559165894985199,
0.047515869140625,
0.06562855839729309,
-0.05937091261148453,
-0.006726075895130634,
0.032472625374794006,
0.006402450613677502,
0.0528288371860981,
-0.03227723017334938,
-0.011792700737714767,
-0.03613993152976036,
0.03234825283288956,
0.0008393615134991705,
-0.030552715063095093,
0.032900191843509674,
0.030697599053382874,
-0.03829939663410187,
0.08122202008962631,
0.03389468044042587,
0.023175369948148727,
-0.01489071175456047,
0.019166886806488037,
0.03609362244606018,
-0.0017037856159731746,
-0.11944852024316788,
0.009850895963609219,
0.03390388935804367,
0.056357014924287796,
0.06589770317077637,
0.0017358909826725721,
-0.038140177726745605,
-0.032720793038606644,
0.07801316678524017,
0.02097213827073574,
-0.044094517827034,
-0.07058695703744888,
0.029713919386267662,
0.048150867223739624,
0.001362498034723103,
0.00018009777704719454,
-0.039257925003767014,
0.031168712303042412,
0.016175558790564537,
-0.016062507405877113,
0.025890236720442772,
0.0440693236887455,
-0.009055632166564465,
-0.04665182903409004,
-0.006092028226703405,
-0.039498861879110336,
0.0006442440208047628,
0.035723891109228134,
0.01282810140401125,
-0.0002851696335710585,
-0.07232261449098587,
0.0110940495505929,
0.09613215178251266,
-0.04015450179576874,
-0.01048236433416605,
0.026895156130194664,
0.06898810714483261,
-0.03272189199924469,
0.12571638822555542,
0.013115660287439823,
0.017283571884036064,
0.06586629897356033,
0.007603781297802925,
0.0070108347572386265,
-0.02718830667436123,
-0.07103952765464783,
0.05813656374812126,
-0.05867625027894974,
-0.040058039128780365,
0.010886098258197308,
-0.01662672683596611,
-0.005271021276712418,
-0.05554506927728653,
-0.009544992819428444,
-0.0278158038854599,
0.09082495421171188,
0.030422953888773918,
-0.02460409514605999,
-0.00810183584690094,
-0.022967956960201263,
-0.02303081750869751,
0.07469385117292404,
0.04975007474422455,
-0.21648980677127838,
0.05405353382229805,
0.07064377516508102,
-0.010771669447422028,
0.010253540240228176,
0.0449436791241169,
0.012619618326425552,
-0.09496201574802399,
-0.05172974243760109,
-0.0035991575568914413,
0.0029631315264850855,
0.01670403778553009,
0.06273675709962845,
0.03811522200703621,
-0.07071225345134735,
-0.007294902577996254,
0.0833590030670166,
0.0006652395823039114,
0.06609886884689331,
-0.015533148311078548,
-0.008589171804487705,
0.008321529254317284,
0.13077621161937714,
0.004148384090512991,
0.006452130153775215,
-0.049424171447753906,
-0.0010507750557735562,
0.04722252115607262,
0.024858688935637474,
0.04369393736124039,
0.020336324349045753,
0.006122077349573374,
0.02864227257668972,
-0.03675873205065727,
0.06037943810224533,
0.02833622694015503,
-0.11288463324308395,
-0.01732686161994934,
0.00717173470184207,
-0.013690647669136524,
-0.017451277002692223,
0.030286218971014023,
-0.04178215563297272,
0.005754068493843079,
0.09272576123476028,
-0.06251048296689987,
-0.02937985770404339,
-0.011631929315626621,
0.03733335807919502,
0.02959788590669632,
-0.04090612381696701,
-0.013206838630139828,
-0.009343096986413002,
-0.017355594784021378,
0.012017910368740559,
-0.061126869171857834,
-0.04942188411951065,
0.002213867148384452,
0.005903775803744793,
0.022990146651864052,
-0.01686178334057331,
-0.06445156037807465,
-0.04506857693195343,
0.012766084633767605,
0.05765347555279732
] |
PMID:27153 | An experimental non-invasive animal technique for measuring nasal airway resistance: I. Adrenergic and antihistaminic agents. | A non-invasive technically simple and sensitive method is described for the determination of nasal airway resistance (NAR). Aerosolized saline elicited a +/- 7% change in baseline NAR, duration 2 minutes, whereas aerosolized histamine increased NAR, duration is greater than 30 minutes. Aerosolized phenylephrine, norepinephrine, and oxymetrazoline elicited dose related decongestive response in a naris subjected to prior congestion (aerosolized histamine). Both H1 and H2 receptors are involved in the congestive response to intranasal histamine. | An experimental non-invasive animal technique for measuring nasal airway resistance: I. Adrenergic and antihistaminic agents. A non-invasive technically simple and sensitive method is described for the determination of nasal airway resistance (NAR). Aerosolized saline elicited a +/- 7% change in baseline NAR, duration 2 minutes, whereas aerosolized histamine increased NAR, duration is greater than 30 minutes. Aerosolized phenylephrine, norepinephrine, and oxymetrazoline elicited dose related decongestive response in a naris subjected to prior congestion (aerosolized histamine). Both H1 and H2 receptors are involved in the congestive response to intranasal histamine. | [
-0.05101698637008667,
0.02433427982032299,
-0.0098195131868124,
0.07776215672492981,
-0.0030150136444717646,
0.03477231040596962,
0.1401582807302475,
-0.0015511780511587858,
-0.09865099936723709,
-0.02879343181848526,
-0.056739769876003265,
-0.039666082710027695,
-0.0017137426184490323,
0.008826584555208683,
-0.007153944578021765,
-0.0363340899348259,
-0.03813096508383751,
-0.021547770127654076,
-0.045372653752565384,
-0.024669045582413673,
-0.043095238506793976,
0.02551048807799816,
0.06837610900402069,
-0.018030306324362755,
0.01299337763339281,
0.03981039673089981,
-0.012010306119918823,
-0.021314939484000206,
-0.05257565155625343,
-0.18314988911151886,
0.039490338414907455,
-0.05024327337741852,
0.009820329025387764,
0.021345229819417,
-0.05524072423577309,
0.047901447862386703,
-0.018672214820981026,
0.021312542259693146,
0.021127959713339806,
0.04671361669898033,
0.02341427095234394,
0.007336182054132223,
0.04558505490422249,
0.020924650132656097,
0.003531632013618946,
-0.009874455630779266,
-0.06969060748815536,
-0.02680470235645771,
0.07199373096227646,
-0.021062497049570084,
0.04679066687822342,
-0.03598150238394737,
0.0379842184484005,
0.006970635149627924,
0.03626393899321556,
-0.017737796530127525,
-0.035984352231025696,
-0.0016488295514136553,
0.015323353931307793,
-0.004350087605416775,
-0.026858661323785782,
0.06591131538152695,
-0.1482112854719162,
0.03679453581571579,
0.03801501542329788,
-0.0051105208694934845,
-0.016989078372716904,
-0.012221220880746841,
0.047816310077905655,
0.0296632032841444,
-0.008723963983356953,
0.01378735713660717,
-0.05825984105467796,
0.02382385917007923,
0.057858388870954514,
0.036609943956136703,
-0.021772343665361404,
-0.01824481040239334,
0.06782171130180359,
-0.053524427115917206,
0.028434064239263535,
0.04514068365097046,
-0.01765802502632141,
-0.03553612902760506,
0.031734269112348557,
-0.0025955706369131804,
0.06087636202573776,
-0.03198522701859474,
-0.022028177976608276,
-0.046164993196725845,
-0.06957747042179108,
-0.001791754737496376,
-0.03417568281292915,
0.019716741517186165,
-0.023246921598911285,
-0.022225115448236465,
-0.0025719187688082457,
0.016717787832021713,
0.0026376540772616863,
0.3780882954597473,
0.009568532928824425,
0.006486264523118734,
-0.09619493037462234,
-0.01882469840347767,
-0.008443777449429035,
-0.017428744584321976,
-0.013872968964278698,
-0.04410911723971367,
-0.03992503508925438,
0.10495836287736893,
0.031905096024274826,
-0.0135145029053092,
0.05347888171672821,
-0.028009982779622078,
0.016212796792387962,
0.01678384840488434,
0.040040627121925354,
0.03592813387513161,
0.031550101935863495,
0.018618809059262276,
-0.0023597150575369596,
-0.04225936532020569,
-0.0053969803266227245,
-0.03693445026874542,
0.003269341541454196,
-0.03419115021824837,
-0.04077045992016792,
0.07379644364118576,
0.040118347853422165,
0.008007748983800411,
0.056459538638591766,
-0.01024538278579712,
-0.04854597896337509,
0.024978533387184143,
0.009747898206114769,
-0.03401673212647438,
0.007803646381944418,
0.014103695750236511,
0.0026709570083767176,
0.012755126692354679,
-0.05288754776120186,
-0.11490733921527863,
0.02699890546500683,
-0.09465619176626205,
-0.11333461850881577,
0.11007347702980042,
-0.03072172775864601,
0.006752440705895424,
-0.04772956296801567,
-0.027670569717884064,
0.01678759977221489,
0.015949567779898643,
-0.05364767834544182,
-0.014808843843638897,
0.016539854928851128,
-0.04889949411153793,
-0.03114292025566101,
-0.01612096279859543,
0.04686589539051056,
0.005130295641720295,
-0.018100669607520103,
-0.0548066720366478,
-0.0016549209831282496,
0.045848164707422256,
-0.03786122798919678,
-0.05779634416103363,
0.01052022073417902,
0.029023129492998123,
0.06606955826282501,
0.030668912455439568,
0.017119785770773888,
-0.0003780024708248675,
-0.03816986829042435,
-0.0387456938624382,
-0.018330330029129982,
-0.0022837044671177864,
0.02744264155626297,
-0.036187924444675446,
0.0368916317820549,
0.0230856966227293,
0.018719958141446114,
-0.01767265424132347,
0.0066359322518110275,
0.017405705526471138,
0.04031161963939667,
0.04240650683641434,
-0.05360431969165802,
-0.004526014439761639,
0.04873582348227501,
0.022473681718111038,
-0.021521136164665222,
-0.022594265639781952,
-0.1171136125922203,
0.02547275274991989,
-0.01116676814854145,
-0.02016817033290863,
-0.008190647698938847,
0.04796215146780014,
0.04613805189728737,
0.054147254675626755,
0.05949648842215538,
0.032850656658411026,
0.06079951673746109,
0.03473428636789322,
0.036009181290864944,
0.07003884017467499,
-0.001222411636263132,
-0.03762737289071083,
0.11900544911623001,
0.001584702404215932,
0.0004667673201765865,
0.030793825164437294,
-0.019293371587991714,
-0.020118828862905502,
-0.04097237437963486,
0.04715089499950409,
0.0382390096783638,
-0.0020754707511514425,
0.0419161431491375,
-0.014168741181492805,
0.01836703158915043,
-0.009118142537772655,
0.004168239887803793,
-0.28661665320396423,
0.012946396134793758,
0.040358442813158035,
-0.025953903794288635,
0.10280851274728775,
-0.03603389114141464,
0.06554988771677017,
-0.0861838161945343,
0.02866761013865471,
-0.03726253658533096,
0.03485911712050438,
0.024986756965517998,
-0.012651928700506687,
0.007145020179450512,
0.010814329609274864,
0.09120603650808334,
0.057715531438589096,
0.0018654519226402044,
0.04492159187793732,
0.01649230159819126,
0.06777787953615189,
-0.006290009245276451,
0.021407848224043846,
-0.013613338582217693,
-0.010342935100197792,
-0.03252008184790611,
0.09153256565332413,
-0.06546195596456528,
0.047849979251623154,
0.005491677206009626,
0.005525296553969383,
0.015793608501553535,
-0.02417687512934208,
-0.08428993821144104,
-0.04392535984516144,
-0.004176158457994461,
-0.0031120602507144213,
0.009992529638111591,
0.0097052538767457,
0.0001702664012555033,
-0.05107758194208145,
0.008800647221505642,
0.028257252648472786,
-0.06501854211091995,
-0.04064564406871796,
-0.02909093350172043,
-0.008346984162926674,
0.00843174010515213,
-0.06694146245718002,
-0.0678875595331192,
0.024284979328513145,
0.024075329303741455,
0.015165372751653194,
-0.02802574820816517,
0.010055742226541042,
-0.00880100205540657,
-0.017768722027540207,
0.00996349286288023,
-0.008929482661187649,
-0.00037604066892527044,
-0.003737138584256172,
0.059666555374860764,
0.05937986820936203,
-0.03114544041454792,
-0.026678061112761497,
0.009882605634629726,
0.022926324978470802,
0.014081339351832867,
0.0073829335160553455,
-0.022349907085299492,
0.030923770740628242,
0.1381351351737976,
-0.05427094176411629,
0.0266072079539299,
0.04133986681699753,
0.031248727813363075,
-0.0013953623129054904,
-0.05390956252813339,
-0.08078575134277344,
0.028000595048069954,
0.015509234741330147,
-0.018748782575130463,
0.027943134307861328,
0.013730219565331936,
-0.0326186828315258,
0.0030432194471359253,
0.001045640790835023,
-0.0259189885109663,
0.03896038234233856,
0.002405955456197262,
0.03276200219988823,
-0.006670714821666479,
0.012654734775424004,
-0.04891321063041687,
0.009909508749842644,
0.04307028651237488,
-0.26490330696105957,
-0.029544582590460777,
0.041467756032943726,
0.013906019739806652,
-0.056268710643053055,
0.006333004683256149,
0.0272359699010849,
-0.022545645013451576,
-0.039193812757730484,
-0.04292258992791176,
-0.0535106398165226,
0.016476480290293694,
0.031911350786685944,
0.061592064797878265,
0.025922929868102074,
0.03930381312966347,
0.036233529448509216,
-0.033302854746580124,
0.012297943234443665,
-0.005175106227397919,
0.015762552618980408,
-0.060160938650369644,
0.13363534212112427,
-0.0006378074758686125,
-0.061643850058317184,
0.026446694508194923,
0.028005443513393402,
-0.012092840857803822,
0.02787412330508232,
-0.03428969532251358,
0.04470838978886604,
-0.06755024194717407,
0.08598662167787552,
-0.040210895240306854,
0.0015719583025202155,
0.04409419745206833,
-0.020240267738699913,
0.04542675241827965,
-0.034570518881082535,
-0.021500080823898315,
-0.05042346566915512,
-0.0008059832034632564,
0.010615154169499874,
-0.05475509539246559,
0.09465627372264862,
-0.04772232472896576,
-0.01572643406689167,
-0.05446874722838402,
-0.045661959797143936,
0.01242906879633665,
-0.033233121037483215,
-0.03733506053686142,
0.0390654057264328,
0.06001470610499382,
-0.014429380185902119,
0.013104764744639397,
-0.023086385801434517,
-0.07476934045553207,
-0.012515672482550144,
0.007689961232244968,
-0.00008716627780813724,
0.02903079427778721,
-0.052604757249355316,
0.004510244820266962,
0.10752391070127487
] |
PMID:27149 | [Nonspecific aorto-arteritis. Anatomo-pathological study of 18 cases]. | The present study comprises a pathological review of 18 necropsy cases with nonspecific aorto-arteritis (Takayasu's disease), autopsied at the Instituto Nacional de Cardiología of Mexico up to 1976. There were 11 females and 7 males with an average age at the time of death of 21.5 years. A comparison according to the distribution and extention of arterial lesions disclosed that our cases are often less classical than those described from Japan. The principal arterial changes were stenosis, occlusion, dilatation and aneurysm formation in the aorta, its branches and main pulmonary artery. More often than not the aorta was extensively involved. The aortic arch syndrome and renovascular hypertension were almost equally present in this serie and an unusual high incidence of coronary artery involvement (33%) leading to myocardial infarction (50%) also was observed. The significance of the intrapulmonary vascular changes in Takayasu's arteritis is discussed. | [Nonspecific aorto-arteritis. Anatomo-pathological study of 18 cases]. The present study comprises a pathological review of 18 necropsy cases with nonspecific aorto-arteritis (Takayasu's disease), autopsied at the Instituto Nacional de Cardiología of Mexico up to 1976. There were 11 females and 7 males with an average age at the time of death of 21.5 years. A comparison according to the distribution and extention of arterial lesions disclosed that our cases are often less classical than those described from Japan. The principal arterial changes were stenosis, occlusion, dilatation and aneurysm formation in the aorta, its branches and main pulmonary artery. More often than not the aorta was extensively involved. The aortic arch syndrome and renovascular hypertension were almost equally present in this serie and an unusual high incidence of coronary artery involvement (33%) leading to myocardial infarction (50%) also was observed. The significance of the intrapulmonary vascular changes in Takayasu's arteritis is discussed. | [
-0.052184466272592545,
0.009546755813062191,
0.014820852316915989,
0.03320853039622307,
-0.031143173575401306,
-0.024707719683647156,
0.0539095439016819,
0.048210084438323975,
-0.013860574923455715,
-0.009092758409678936,
-0.006619960535317659,
-0.05830920487642288,
-0.025019973516464233,
0.014872102066874504,
-0.0343150869011879,
-0.019321167841553688,
-0.015449262224137783,
-0.013873614370822906,
-0.030271848663687706,
0.03597508370876312,
-0.033571384847164154,
0.03690871223807335,
0.029551787301898003,
-0.032848577946424484,
-0.0008426524582318962,
0.015416106209158897,
0.0487687848508358,
-0.02428840473294258,
-0.005063131917268038,
-0.1345868855714798,
-0.010697385296225548,
-0.08495571464300156,
-0.024583810940384865,
0.05259586498141289,
-0.019490042701363564,
0.021754521876573563,
-0.04594762250781059,
0.04605548456311226,
0.014028172008693218,
-0.02497268281877041,
-0.010474196635186672,
0.03262131288647652,
0.013232029974460602,
0.008926955983042717,
0.02326083555817604,
0.02181561477482319,
-0.047354914247989655,
0.0015924343606457114,
0.10473927855491638,
0.03694303333759308,
-0.047860849648714066,
0.0049024117179214954,
0.041848111897706985,
0.09676727652549744,
0.0018760139355435967,
-0.03996550291776657,
0.016985522583127022,
-0.012783986516296864,
0.04153923690319061,
-0.025997435674071312,
0.043265122920274734,
-0.003533009672537446,
-0.11197371780872345,
0.059994809329509735,
-0.02719658985733986,
0.013378337025642395,
0.014709193259477615,
-0.014028183184564114,
0.06470787525177002,
-0.046051573008298874,
-0.02663431689143181,
0.03543177247047424,
-0.027331221848726273,
0.06782864034175873,
0.03529052063822746,
-0.01070382073521614,
-0.046745527535676956,
-0.030722135677933693,
-0.001873323693871498,
0.001454503508284688,
0.03843815624713898,
0.043063994497060776,
-0.04471282660961151,
-0.002915759803727269,
-0.029074804857373238,
-0.006973680108785629,
-0.003487739944830537,
-0.09165351092815399,
-0.09739723801612854,
0.01989978365600109,
-0.05551689863204956,
-0.031212667003273964,
-0.015208653174340725,
0.017641589045524597,
-0.01620999164879322,
-0.03933687508106232,
0.0029059431981295347,
-0.022924447432160378,
0.004712809808552265,
0.40591999888420105,
0.009258807636797428,
-0.01668333262205124,
-0.012660439126193523,
0.019270632416009903,
0.03237899765372276,
0.034651149064302444,
-0.03358586132526398,
-0.03724055737257004,
-0.039500173181295395,
0.014464168809354305,
0.02582281082868576,
-0.020882517099380493,
0.05773378908634186,
0.02730594575405121,
0.011188036762177944,
0.00781367439776659,
0.019201653078198433,
0.01689114235341549,
-0.05515116825699806,
0.08113440126180649,
0.00980752520263195,
-0.03578510880470276,
0.005428718868643045,
-0.04522387310862541,
0.07666032761335373,
0.0008189476793631911,
0.02969735860824585,
0.05601881444454193,
0.06967394798994064,
-0.005684820935130119,
0.05679266154766083,
0.009278486482799053,
-0.06143411621451378,
-0.013773658312857151,
0.043961361050605774,
0.013123438693583012,
-0.02191251888871193,
0.0258782971650362,
0.001588928746059537,
0.010712146759033203,
-0.01088040042668581,
-0.05179032310843468,
-0.008069927804172039,
-0.06563369929790497,
-0.07117221504449844,
0.09237681329250336,
0.03151627257466316,
0.040093570947647095,
-0.04192180559039116,
0.0045576575212180614,
-0.017102649435400963,
-0.013537457212805748,
-0.02964889444410801,
0.025968516245484352,
-0.02677830308675766,
-0.04977530613541603,
-0.03741098940372467,
0.06971533596515656,
-0.01740351878106594,
0.006518984213471413,
-0.0319838747382164,
-0.029879342764616013,
-0.008373293094336987,
0.07362430542707443,
0.030821483582258224,
-0.022801144048571587,
-0.026671864092350006,
0.00928084459155798,
0.05169204622507095,
-0.00009129710088018328,
-0.029752640053629875,
0.005423597525805235,
-0.002864144742488861,
0.015779493376612663,
0.0892491340637207,
0.07953930646181107,
0.03513922542333603,
-0.041649624705314636,
-0.0077809737995266914,
-0.025085914880037308,
0.0468251071870327,
0.09212590754032135,
-0.01918764039874077,
0.062377456575632095,
0.012318767607212067,
-0.002656392753124237,
-0.046760715544223785,
-0.029144303873181343,
0.04623180627822876,
0.003927046898752451,
0.004367511719465256,
-0.05289381369948387,
-0.11638669669628143,
0.006024711299687624,
0.013617007061839104,
-0.05710760876536369,
0.008077563717961311,
0.0015187427634373307,
0.030621500685811043,
0.0003343088610563427,
0.07727169245481491,
0.0064176651649177074,
0.0152015071362257,
-0.025371985509991646,
0.013078493997454643,
0.041407085955142975,
0.0005745998350903392,
-0.014087979681789875,
-0.06748087704181671,
-0.007854733616113663,
-0.028955385088920593,
0.022776447236537933,
-0.04826395586133003,
0.08469416946172714,
0.013710579834878445,
0.023865511640906334,
0.012447326444089413,
-0.01009000837802887,
0.040361590683460236,
0.04677209630608559,
0.052806414663791656,
-0.050243329256772995,
0.005252786912024021,
-0.2928779721260071,
-0.04720837622880936,
-0.027104564011096954,
-0.038778167217969894,
0.029244940727949142,
-0.035239994525909424,
-0.021945741027593613,
-0.0013725021854043007,
0.036928512156009674,
0.04602552577853203,
-0.02886095829308033,
0.07193561643362045,
-0.035181451588869095,
-0.04245084151625633,
-0.010577507317066193,
0.12514005601406097,
0.05592579394578934,
-0.06824295222759247,
-0.026262719184160233,
-0.03781585767865181,
0.007337899878621101,
-0.020167917013168335,
-0.005064030177891254,
0.015891054645180702,
0.08818938583135605,
0.009173321537673473,
0.14682094752788544,
-0.003244982799515128,
-0.008582446724176407,
-0.042078785598278046,
0.00036337986239232123,
-0.04402187466621399,
-0.05337783694267273,
-0.03943825513124466,
0.05631551146507263,
-0.03749828785657883,
-0.0019915467128157616,
-0.052926551550626755,
-0.045640889555215836,
0.027454691007733345,
-0.037291351705789566,
-0.02223069965839386,
-0.00664050318300724,
-0.0012552886037155986,
-0.06812615692615509,
-0.05299298092722893,
-0.01740541122853756,
0.029603617265820503,
0.011500611901283264,
0.052632588893175125,
-0.010413628071546555,
0.0032211935613304377,
0.0024166302755475044,
0.020905839279294014,
0.021831557154655457,
-0.014146029017865658,
-0.0463314987719059,
0.03203688934445381,
-0.0011899538803845644,
-0.047955505549907684,
-0.040665335953235626,
0.03569023311138153,
0.06568214297294617,
-0.08518961071968079,
-0.009892705827951431,
0.010831756517291069,
0.037243980914354324,
0.012423807755112648,
0.05373059958219528,
-0.0015215836465358734,
-0.0517495758831501,
0.08931013196706772,
-0.013723865151405334,
-0.012184059247374535,
0.06998690217733383,
0.043770983815193176,
0.015090378932654858,
-0.04227583482861519,
-0.014259794726967812,
0.040081582963466644,
0.01627923920750618,
-0.05990593507885933,
0.03100101836025715,
0.041355688124895096,
-0.0638522133231163,
0.03716214373707771,
-0.012225097045302391,
-0.03571251407265663,
-0.04546644166111946,
-0.0037911362014710903,
0.038660190999507904,
-0.04618554562330246,
-0.046241264790296555,
0.028294414281845093,
-0.027105016633868217,
-0.0077606746926903725,
-0.27070748805999756,
-0.02430511638522148,
0.021614862605929375,
-0.04306330904364586,
-0.04496696963906288,
-0.0070202103815972805,
-0.06177033856511116,
-0.04340112954378128,
-0.016368340700864792,
0.06708606332540512,
0.006655641831457615,
-0.006307756993919611,
0.05290625989437103,
-0.0009701811359263957,
-0.011574063450098038,
0.0011783939553424716,
0.0018885029712691903,
-0.02564694918692112,
0.05300980806350708,
-0.06307812035083771,
-0.029252292588353157,
-0.007717649918049574,
0.1299145370721817,
0.03427356854081154,
-0.07945367693901062,
0.014007615856826305,
-0.007859566248953342,
0.04885964095592499,
-0.06395213305950165,
-0.012504647485911846,
0.03246481344103813,
-0.03745020925998688,
0.054058823734521866,
-0.00643163500353694,
0.0597129724919796,
0.06322220712900162,
-0.018428049981594086,
0.11305118352174759,
-0.051937349140644073,
-0.008658399805426598,
-0.03789220750331879,
-0.008290608413517475,
-0.05888744816184044,
-0.006605721544474363,
0.07390598207712173,
0.0409245602786541,
-0.02926657535135746,
0.03661675006151199,
0.05142921209335327,
0.04382581263780594,
-0.009552833624184132,
0.02817796729505062,
0.032038502395153046,
0.027736522257328033,
0.06220051273703575,
-0.011014100164175034,
-0.014936059713363647,
-0.04186652600765228,
0.008109938353300095,
-0.05532589554786682,
0.02377946488559246,
0.03167317435145378,
-0.09368501603603363,
0.08655676245689392,
0.030133910477161407
] |
PMID:27154 | Age-dependence of renal tubular reabsorption of nitrofurantoin. | Nitrofurantoin is rapidly excreted in adult rats. However, in 5 to 15 days old rats the renal excretion of nitrofurantoin is diminshed by a high rate of reabsorption. Because nitrofurantoin has a pKa of 7.2 its tubular reabsorption is influenced by the urine pH but is independent of urine flow. About 50% of an administered dose of nitrofurantoin is recovered as metabolites in the urine of both young and adult rats. Probenecid blocks the active tubular secretion of nitrofurantoin independently of age and reduces its rate of renal clearance. Induction of drug metabolizing enzymes by pretreatment with phenobarbital was without effect either on the metabolism or renal excretion of nitrofurantoin nor could the renal excretion of this drug be diminished by repeated administration. The age-dependent difference in the rate of renal excretion of nitrofurantion is due to the high rate of tubular reabsorption in the young animals. This study provides experimental support for the therapeutic use of nitrofurantoin in the neonatal period. | Age-dependence of renal tubular reabsorption of nitrofurantoin. Nitrofurantoin is rapidly excreted in adult rats. However, in 5 to 15 days old rats the renal excretion of nitrofurantoin is diminshed by a high rate of reabsorption. Because nitrofurantoin has a pKa of 7.2 its tubular reabsorption is influenced by the urine pH but is independent of urine flow. About 50% of an administered dose of nitrofurantoin is recovered as metabolites in the urine of both young and adult rats. Probenecid blocks the active tubular secretion of nitrofurantoin independently of age and reduces its rate of renal clearance. Induction of drug metabolizing enzymes by pretreatment with phenobarbital was without effect either on the metabolism or renal excretion of nitrofurantoin nor could the renal excretion of this drug be diminished by repeated administration. The age-dependent difference in the rate of renal excretion of nitrofurantion is due to the high rate of tubular reabsorption in the young animals. This study provides experimental support for the therapeutic use of nitrofurantoin in the neonatal period. | [
-0.04042259603738785,
-0.019218947738409042,
0.020150046795606613,
-0.02112579345703125,
0.014287677593529224,
-0.011913063004612923,
0.04857539013028145,
0.03301926329731941,
-0.06948033720254898,
-0.015058184042572975,
-0.000012227011211507488,
-0.037145353853702545,
0.016228094696998596,
0.07202378660440445,
-0.04372824355959892,
0.018556077033281326,
0.03292513266205788,
0.008868026547133923,
-0.05166720598936081,
-0.002217424800619483,
0.020413106307387352,
-0.020997213199734688,
0.0668555423617363,
0.028902161866426468,
0.032191142439842224,
-0.005468266550451517,
0.03340831771492958,
0.010630660690367222,
-0.03036886267364025,
-0.21057337522506714,
0.06742887943983078,
-0.021701978519558907,
-0.011446972377598286,
-0.04538535699248314,
-0.01243946049362421,
0.033954620361328125,
-0.02193329855799675,
0.024916326627135277,
-0.02153104543685913,
0.038229458034038544,
0.0006718674558214843,
0.0638139396905899,
-0.03535487875342369,
-0.008361883461475372,
0.029823532328009605,
-0.021670537069439888,
-0.04670363664627075,
0.03604607656598091,
0.13773523271083832,
0.017177732661366463,
-0.007802839856594801,
-0.008080340921878815,
0.06677169352769852,
0.0732373297214508,
0.0015311239985749125,
-0.014317771419882774,
0.03170732036232948,
-0.015012256801128387,
0.018058858811855316,
0.05223705247044563,
0.021869128569960594,
0.020733684301376343,
-0.14185073971748352,
0.05808093398809433,
-0.00028015143470838666,
-0.004558608867228031,
0.007325540762394667,
-0.024066302925348282,
0.012896579690277576,
0.02496631257236004,
-0.019499873742461205,
0.015906907618045807,
-0.06128879263997078,
0.010690578259527683,
0.019552651792764664,
-0.0097119165584445,
-0.020318925380706787,
-0.017202654853463173,
0.04555691033601761,
-0.021522173658013344,
0.052421167492866516,
0.03419310972094536,
0.006986059248447418,
-0.009661093354225159,
0.023573489859700203,
-0.010699061676859856,
0.035208895802497864,
-0.030583981424570084,
0.011978480964899063,
0.031880881637334824,
0.03040051832795143,
0.02476768009364605,
-0.02828238531947136,
-0.0667584240436554,
-0.03363383561372757,
-0.003608915489166975,
0.027946315705776215,
-0.006286012940108776,
-0.026264911517500877,
0.32817813754081726,
-0.007633613888174295,
0.04100370407104492,
-0.08064305782318115,
0.02097220905125141,
0.008642507717013359,
-0.02445806749165058,
0.03218170255422592,
0.03317039832472801,
0.03472495451569557,
0.06591544300317764,
0.035840075463056564,
0.021444397047162056,
0.08019068837165833,
-0.05176519602537155,
-0.05666554719209671,
0.006721474230289459,
0.0381556861102581,
0.04231950640678406,
0.005853570997714996,
0.06127724051475525,
-0.0017637646524235606,
0.011297113262116909,
0.03476881608366966,
-0.037835847586393356,
0.03753925859928131,
0.02435481548309326,
-0.016037626191973686,
0.05899324268102646,
-0.030498411506414413,
-0.011531713418662548,
0.021117758005857468,
-0.017652960494160652,
0.010410291142761707,
-0.004737059585750103,
0.00278850132599473,
-0.029680605977773666,
0.02163059078156948,
-0.0635596513748169,
-0.017267467454075813,
0.020213887095451355,
-0.08121233433485031,
-0.08436998724937439,
-0.04988808184862137,
-0.05063806101679802,
-0.08748353272676468,
0.1281038522720337,
0.029773656278848648,
0.01528202835470438,
-0.009092832915484905,
-0.010982375591993332,
-0.015677236020565033,
0.08290393650531769,
-0.0074562979862093925,
-0.002019293839111924,
-0.048510875552892685,
0.043585531413555145,
-0.0436810739338398,
-0.012981008738279343,
-0.0309501551091671,
0.012707280926406384,
-0.04851997271180153,
-0.08150576055049896,
-0.07779677957296371,
0.0346989706158638,
0.0007164468988776207,
0.051736894994974136,
-0.010731888003647327,
0.019573906436562538,
0.02442595176398754,
0.07328788936138153,
-0.02365781180560589,
-0.015124162659049034,
-0.015120003372430801,
-0.007407317403703928,
-0.05597933009266853,
-0.02785147912800312,
0.053464654833078384,
0.00037910431274212897,
0.02286878228187561,
0.008058443665504456,
-0.018513323739171028,
-0.03260591998696327,
-0.014013521373271942,
0.020231714472174644,
0.035643428564071655,
-0.04329673573374748,
-0.008184983395040035,
-0.02460976131260395,
-0.014648332260549068,
0.03427280858159065,
0.0019560547079890966,
0.030342567712068558,
-0.0709749162197113,
0.028612850233912468,
-0.057445090264081955,
0.006312879733741283,
-0.06694011390209198,
0.006690943147987127,
-0.027042889967560768,
-0.060270849615335464,
0.11244428157806396,
0.0032963305711746216,
0.007098773494362831,
0.03779012709856033,
0.009869798086583614,
0.042563457041978836,
0.02959919534623623,
-0.012390525080263615,
0.04768858850002289,
-0.046253520995378494,
-0.021267354488372803,
0.009971756488084793,
-0.022873129695653915,
-0.03596700355410576,
0.0022294288501143456,
0.012960059568285942,
-0.04622845724225044,
0.0042673214338719845,
0.07203014940023422,
0.011456967331469059,
0.04817104712128639,
-0.03295714408159256,
0.026843588799238205,
-0.29495128989219666,
0.0027808924205601215,
0.06147918105125427,
0.013993744738399982,
0.10366258770227432,
-0.08793410658836365,
-0.02619808167219162,
-0.01740582473576069,
-0.029757561162114143,
-0.04239292070269585,
0.05735619366168976,
0.06391572952270508,
-0.028193650767207146,
0.01891251839697361,
-0.021614694967865944,
0.059659164398908615,
0.04806338623166084,
0.014284176751971245,
0.007097594905644655,
-0.026719726622104645,
0.0212781373411417,
-0.09067176282405853,
0.038500357419252396,
0.019427059218287468,
0.07358641177415848,
-0.04391708970069885,
0.08038930594921112,
-0.02331155352294445,
-0.048109445720911026,
-0.027138693258166313,
0.05175076425075531,
-0.039524540305137634,
-0.04698673635721207,
-0.11626327782869339,
-0.004348090384155512,
0.025917453691363335,
0.013469239696860313,
-0.03450162336230278,
0.044935617595911026,
-0.041978348046541214,
-0.06002163514494896,
0.05361232906579971,
0.04276301711797714,
-0.049841318279504776,
-0.051420360803604126,
-0.03858480229973793,
0.006386310327798128,
0.05340332165360451,
0.019774800166487694,
-0.06032705679535866,
0.01727628894150257,
0.0628417357802391,
-0.012369953095912933,
-0.00676962872967124,
0.030401630327105522,
-0.03250022977590561,
-0.039462611079216,
0.015241802670061588,
-0.009588377550244331,
-0.03815193474292755,
-0.054373204708099365,
0.07723085582256317,
0.00793367251753807,
-0.07497598230838776,
-0.006184997968375683,
0.00021317193750292063,
-0.015053534880280495,
0.006107913795858622,
0.061751555651426315,
0.05140848830342293,
0.039641376584768295,
0.1261177808046341,
-0.09170647710561752,
0.07227776944637299,
0.03582734614610672,
0.013450794853270054,
-0.014058488421142101,
-0.009516995400190353,
-0.038920141756534576,
0.011378683149814606,
-0.033057816326618195,
-0.07012529671192169,
0.047796715050935745,
0.006009818986058235,
0.021964553743600845,
-0.02434413507580757,
0.014001185074448586,
-0.039559029042720795,
0.04868598282337189,
0.05835985764861107,
-0.02011311985552311,
0.034957144409418106,
-0.0815289169549942,
-0.04690678045153618,
0.004333443474024534,
0.06642168760299683,
-0.29008981585502625,
0.003533006180077791,
-0.008280094712972641,
-0.013155607506632805,
0.05037372186779976,
0.04056699573993683,
0.017755132168531418,
-0.019116681069135666,
0.013193749822676182,
0.020744597539305687,
0.053873177617788315,
0.03516058996319771,
0.060538191348314285,
0.0417398177087307,
0.03195313736796379,
-0.02111050859093666,
0.057598717510700226,
-0.006804826203733683,
-0.027204710990190506,
-0.03992776572704315,
0.06327631324529648,
-0.0025441660545766354,
0.0964212566614151,
-0.021338090300559998,
-0.008908758871257305,
0.04064813256263733,
-0.09317075461149216,
0.05743425711989403,
0.023846518248319626,
-0.03507867082953453,
-0.04267504811286926,
0.03444458171725273,
0.05168915539979935,
-0.05407165735960007,
0.0424240380525589,
0.011207282543182373,
-0.04815862700343132,
-0.026157839223742485,
-0.06903952360153198,
-0.012903975322842598,
-0.05572718009352684,
0.012094013392925262,
-0.05725182965397835,
0.004664561711251736,
0.05221080407500267,
-0.06160895153880119,
-0.049955449998378754,
-0.04225046932697296,
0.03559885919094086,
0.011759708635509014,
-0.014065737836062908,
-0.0044566914439201355,
0.016327882185578346,
0.014538669027388096,
0.08335356414318085,
-0.014151116833090782,
-0.009464509785175323,
-0.06075607240200043,
-0.011171230114996433,
-0.05869850888848305,
-0.07512500882148743,
0.007527303881943226,
-0.032527901232242584,
0.022448929026722908,
0.00920340046286583
] |
PMID:27155 | Hypersensitivity to salicylazosulfapyridine: renal and hepatic toxic reactions. | Salicylazosulfapyridine has been used for a number of years as therapy for ulcerative colitis. Reported toxicities are usually minor. This case report represents an acute allergic reaction to the drug. Characterized by fever, rash, eosinophilia, nephritis, and hepatitis. Resolution occurred with discontinuation of salicylazosulfapyridine. Although similar reactions have been reported with the antimicrobial sulfonamides, none has been fully described with salicylazosulfapyridine, a combination of a sulfonamide and salicylate. | Hypersensitivity to salicylazosulfapyridine: renal and hepatic toxic reactions. Salicylazosulfapyridine has been used for a number of years as therapy for ulcerative colitis. Reported toxicities are usually minor. This case report represents an acute allergic reaction to the drug. Characterized by fever, rash, eosinophilia, nephritis, and hepatitis. Resolution occurred with discontinuation of salicylazosulfapyridine. Although similar reactions have been reported with the antimicrobial sulfonamides, none has been fully described with salicylazosulfapyridine, a combination of a sulfonamide and salicylate. | [
-0.019760819151997566,
0.023310460150241852,
0.015917908400297165,
0.03383302316069603,
0.010713842697441578,
-0.0005817045457661152,
0.06411110609769821,
0.06180626526474953,
-0.09535331279039383,
-0.07369086146354675,
0.039079129695892334,
0.025202734395861626,
0.01977895386517048,
0.018891075626015663,
0.00886689405888319,
0.02870282717049122,
0.03596622496843338,
-0.03500036895275116,
-0.07174324989318848,
-0.05188987776637077,
-0.01810162328183651,
0.02751767635345459,
0.013844732195138931,
-0.01923060603439808,
0.014283165335655212,
0.004317217972129583,
0.008758686482906342,
0.05261457711458206,
-0.07441676408052444,
-0.14447763562202454,
0.023934895172715187,
0.0057226140052080154,
-0.03200685977935791,
-0.036109086126089096,
-0.035780169069767,
-0.007566626183688641,
-0.033708471804857254,
-0.003740884130820632,
-0.02557593584060669,
0.012010179460048676,
-0.046625010669231415,
0.010123527608811855,
0.016352834179997444,
0.02763807773590088,
-0.05607951059937477,
-0.036448098719120026,
-0.013277281075716019,
-0.022786080837249756,
0.13086242973804474,
0.00836643110960722,
0.03147844597697258,
-0.03417389094829559,
0.03533438220620155,
0.06946523487567902,
0.024473657831549644,
0.0004985075793229043,
0.012843937613070011,
0.018495410680770874,
-0.0282469280064106,
0.0328650176525116,
0.010436004027724266,
0.03614838421344757,
-0.10055779665708542,
0.06460637599229813,
0.006212035194039345,
0.00971872266381979,
-0.020808065310120583,
-0.019102316349744797,
0.09280464798212051,
0.1013006716966629,
-0.012608385644853115,
0.028095871210098267,
-0.02467494085431099,
0.0761004164814949,
0.008923202753067017,
0.049078814685344696,
-0.014632733538746834,
-0.030757401138544083,
0.07415399700403214,
0.060362398624420166,
0.01856343261897564,
-0.0019389103399589658,
-0.029903888702392578,
-0.09684613347053528,
0.05444592237472534,
-0.05222909897565842,
0.030171234160661697,
0.021392975002527237,
0.011715732514858246,
-0.02649465575814247,
0.008682089857757092,
-0.015367943793535233,
-0.0005159382126294076,
-0.07170150429010391,
-0.031512483954429626,
-0.006102667190134525,
-0.02342790737748146,
-0.00878690555691719,
-0.02164635993540287,
0.32896170020103455,
-0.011092707514762878,
-0.025695865973830223,
-0.05972755327820778,
0.052626803517341614,
0.021082477644085884,
0.031943418085575104,
0.0033915454987436533,
-0.007638227194547653,
0.00045545276952907443,
0.001411594683304429,
0.0681699886918068,
-0.059718187898397446,
0.008210532367229462,
-0.012655626982450485,
-0.04026787728071213,
0.018597222864627838,
0.0670233964920044,
-0.00560558820143342,
-0.043922655284404755,
0.021490665152668953,
0.04007192328572273,
-0.02204207517206669,
0.025050874799489975,
-0.002159764291718602,
0.06084052845835686,
0.015533179976046085,
0.030511099845170975,
0.06973841041326523,
-0.01147338654845953,
0.05163915827870369,
0.05857345834374428,
-0.05451665446162224,
0.004173459019511938,
0.027992283925414085,
-0.007887239567935467,
0.007597557734698057,
0.03556906431913376,
-0.04889095947146416,
0.07870218902826309,
0.08370383083820343,
-0.01322361920028925,
-0.1104673221707344,
-0.057924214750528336,
-0.08512597531080246,
-0.04565306380391121,
0.0654161348938942,
0.015820086002349854,
-0.02109372802078724,
-0.062041040509939194,
0.049768391996622086,
-0.037995461374521255,
0.01756162941455841,
-0.011575317941606045,
0.04373379796743393,
-0.052932944148778915,
-0.05591585114598274,
-0.011637834832072258,
0.04511633142828941,
0.023840397596359253,
-0.01853371039032936,
-0.039117615669965744,
-0.08378244936466217,
-0.0090472511947155,
0.09740901738405228,
-0.05395647510886192,
0.015602216124534607,
-0.04211720824241638,
-0.01431330293416977,
-0.020386721938848495,
0.06747899204492569,
0.01932649314403534,
-0.03705433011054993,
0.049651313573122025,
-0.06974882632493973,
-0.026941683143377304,
0.05461905524134636,
0.029403096064925194,
0.009753799065947533,
0.012390370480716228,
-0.02375497668981552,
0.0037333357613533735,
0.027141492813825607,
-0.007548851426690817,
0.05488663539290428,
-0.007597141433507204,
-0.06558188050985336,
-0.02056228742003441,
0.03156072273850441,
0.05489468574523926,
0.030359070748090744,
-0.05374371260404587,
-0.007559423800557852,
-0.06954589486122131,
0.011438572779297829,
-0.0375828854739666,
0.08120225369930267,
-0.09321831911802292,
0.033693697303533554,
0.010945671238005161,
0.023142380639910698,
0.1276702880859375,
0.08271182328462601,
0.03392861783504486,
0.06736043840646744,
-0.00031146311084739864,
-0.020217295736074448,
-0.02648904360830784,
0.03413573279976845,
-0.004496165085583925,
-0.020591963082551956,
-0.0015551334945484996,
0.07483717799186707,
-0.010639729909598827,
0.010133524425327778,
-0.026174964383244514,
0.03286169469356537,
0.007820850238204002,
0.028170762583613396,
0.029316432774066925,
-0.03301355615258217,
0.015285501256585121,
0.02985309436917305,
-0.09052109718322754,
-0.29504483938217163,
0.00010964993271045387,
-0.012726649641990662,
-0.03601262345910072,
0.026550037786364555,
-0.053562842309474945,
-0.030572600662708282,
-0.055000267922878265,
0.00015144846111070365,
-0.002583725843578577,
0.05542149394750595,
0.06557881832122803,
-0.01112887728959322,
-0.09966322034597397,
-0.012759195640683174,
0.020350659266114235,
0.0009461402660235763,
0.013675701804459095,
-0.005125370807945728,
0.057699427008628845,
-0.015196563675999641,
-0.039322394877672195,
0.02544602006673813,
0.01576913893222809,
0.03679798170924187,
0.009588276967406273,
0.11488986015319824,
0.021134480834007263,
-0.04556681960821152,
-0.06900524348020554,
0.03842657804489136,
-0.016021789982914925,
0.0027288629207760096,
-0.07277573645114899,
-0.023177390918135643,
-0.0003445710754022002,
-0.03512300178408623,
-0.03074931725859642,
0.02289651334285736,
-0.005373404826968908,
-0.0490555576980114,
-0.04173550009727478,
0.007635516580194235,
-0.06906764954328537,
-0.0028293973300606012,
0.03152696043252945,
-0.02532638981938362,
0.002452642424032092,
0.02719404548406601,
0.017176685854792595,
0.05147861689329147,
0.1010812297463417,
-0.00965827889740467,
0.021302640438079834,
0.07773901522159576,
-0.037503890693187714,
-0.03662283346056938,
0.003106293734163046,
-0.05350146442651749,
-0.0630105584859848,
0.00891876220703125,
0.02065454237163067,
0.09372692555189133,
-0.05139683187007904,
-0.01923254318535328,
-0.017693623900413513,
-0.004824617877602577,
-0.042402587831020355,
0.016201665624976158,
0.051952917128801346,
-0.007486802525818348,
0.1890852153301239,
-0.017180994153022766,
-0.023216571658849716,
0.024709457531571388,
0.08634818345308304,
-0.05480249971151352,
-0.010993758216500282,
-0.07136042416095734,
0.037059202790260315,
-0.012841541320085526,
-0.03935873880982399,
0.0399935320019722,
0.009963670745491982,
-0.04071010276675224,
-0.006903441157191992,
-0.04963139072060585,
-0.017643168568611145,
0.030115719884634018,
-0.004452208057045937,
0.019782811403274536,
0.005987606942653656,
-0.0454467311501503,
0.004662686958909035,
0.02477237768471241,
0.013319014571607113,
-0.2425999492406845,
-0.02794474922120571,
-0.016067350283265114,
-0.012238426133990288,
-0.017920035868883133,
-0.004311892669647932,
0.014340904541313648,
-0.03127337619662285,
-0.04789922758936882,
-0.00896002259105444,
0.05530603975057602,
0.06132131069898605,
0.03194058686494827,
-0.006251069717109203,
0.013186725787818432,
-0.005714136175811291,
0.06818877160549164,
0.00029614538652822375,
-0.0031276654917746782,
-0.006831645499914885,
0.008440163917839527,
-0.005294916220009327,
0.14736957848072052,
0.0034678862430155277,
-0.04000614956021309,
0.052505288273096085,
0.01705336384475231,
0.044627536088228226,
-0.06350095570087433,
0.014485971070826054,
-0.009244746528565884,
0.02774161845445633,
-0.01573103852570057,
-0.029116904363036156,
0.06051903963088989,
0.02604038082063198,
0.01675678789615631,
-0.055910155177116394,
-0.08165749907493591,
0.023412620648741722,
-0.056889329105615616,
0.0677383542060852,
-0.07206679880619049,
-0.019813556224107742,
0.02475936897099018,
0.016155457124114037,
-0.030241167172789574,
-0.07237011194229126,
-0.011298035271465778,
-0.005414008162915707,
-0.005063031334429979,
-0.04368773475289345,
0.017982343211770058,
0.04428106173872948,
0.016923490911722183,
-0.07313253730535507,
0.0041685630567371845,
-0.0548437274992466,
-0.005346992518752813,
-0.06200282275676727,
0.006769390776753426,
0.041165050119161606,
-0.06648772209882736,
0.024247601628303528,
0.027780592441558838
] |
PMID:27156 | [The myocardiopathies of dermatomyositis and periarteritis nodosa]. | Myocardial involvement was indicated by isolated findings (especially on the ECG) without corresponding functional disorders in one third of cases of dermatomyositis. Rapidly fatal heart failure occurred rarely in this disease, and was noteworthy for a QS complex in the central precordial leads, and for the presence of intracardiac thromboses which could give rise to emboli. Terminal cardiac failure was the rule in cases of periarteritis nodosa, which is a much rarer disease; this occurrence was secondary to hypertension. The most characteristic lesions were nodular coronary arteritis and disseminated myocarditis due to the arteriolar lesions. | [The myocardiopathies of dermatomyositis and periarteritis nodosa]. Myocardial involvement was indicated by isolated findings (especially on the ECG) without corresponding functional disorders in one third of cases of dermatomyositis. Rapidly fatal heart failure occurred rarely in this disease, and was noteworthy for a QS complex in the central precordial leads, and for the presence of intracardiac thromboses which could give rise to emboli. Terminal cardiac failure was the rule in cases of periarteritis nodosa, which is a much rarer disease; this occurrence was secondary to hypertension. The most characteristic lesions were nodular coronary arteritis and disseminated myocarditis due to the arteriolar lesions. | [
-0.03181147575378418,
-0.05165056139230728,
0.018649877980351448,
0.05190558359026909,
-0.004136126954108477,
-0.07547741383314133,
0.055646173655986786,
0.05016404762864113,
-0.025234486907720566,
-0.01247034315019846,
0.0008607421768829226,
-0.05996748432517052,
0.0029659548308700323,
0.014115791767835617,
-0.05407899245619774,
-0.03692580759525299,
0.023233884945511818,
0.02079075574874878,
-0.022797850891947746,
0.026253055781126022,
-0.10546556860208511,
0.06177029386162758,
-0.023696864023804665,
-0.05156164616346359,
-0.008290871046483517,
0.014899584464728832,
0.038349803537130356,
0.009917651303112507,
-0.06587738543748856,
-0.11249413341283798,
-0.027249416336417198,
-0.021281590685248375,
-0.025004534050822258,
-0.0009838170371949673,
-0.015357821248471737,
-0.044117581099271774,
-0.044671252369880676,
-0.008060108870267868,
-0.03412939980626106,
-0.024393731728196144,
0.01895100437104702,
-0.006294985301792622,
0.0220472551882267,
0.013034052215516567,
0.031035151332616806,
-0.0065824855118989944,
-0.048474811017513275,
-0.007240742444992065,
0.16126973927021027,
0.010058854706585407,
-0.0579175129532814,
0.021285852417349815,
0.062367912381887436,
0.0909370705485344,
0.01793665438890457,
0.009927275590598583,
0.042584825307130814,
0.0026555454824119806,
0.026056593284010887,
0.0030218164902180433,
0.024851690977811813,
0.01659117452800274,
-0.10949534922838211,
0.0016085857059806585,
-0.0033649078104645014,
-0.040019575506448746,
0.02289671264588833,
-0.0025854555424302816,
0.027447277680039406,
0.03966718539595604,
-0.008798191323876381,
0.05728234723210335,
0.011192104779183865,
0.05596679076552391,
-0.01911049522459507,
-0.01272248849272728,
0.025833647698163986,
0.04043293744325638,
0.022808963432908058,
0.04978707805275917,
-0.05551132187247276,
-0.0006376787205226719,
-0.011936619877815247,
-0.07356486469507217,
-0.03514247015118599,
0.0016980867367237806,
0.03337553143501282,
-0.07615870982408524,
-0.00343696610070765,
0.02784009650349617,
-0.0009397321846336126,
-0.023701036348938942,
0.0026199829299002886,
0.0274207703769207,
-0.030848456546664238,
0.017209000885486603,
-0.01802665740251541,
-0.008806790225207806,
-0.02231857180595398,
0.38767144083976746,
0.04271876439452171,
-0.027042999863624573,
0.000385668856324628,
0.05371016263961792,
-0.014982258901000023,
0.01881548948585987,
0.025932347401976585,
-0.03302432969212532,
-0.042635127902030945,
-0.002549485769122839,
0.030765866860747337,
-0.029172642156481743,
0.02086007222533226,
0.04064079374074936,
0.05738939717411995,
0.019621774554252625,
0.03361104056239128,
0.022970473393797874,
-0.04671128839254379,
0.08591272681951523,
0.0058814710937440395,
-0.02806258574128151,
0.05461505800485611,
-0.012034584768116474,
0.004551011603325605,
-0.006440173368901014,
0.007584732491523027,
0.07909214496612549,
0.0886373370885849,
-0.012955328449606895,
0.04572371393442154,
0.06087931618094444,
-0.06938249617815018,
-0.01056494191288948,
0.0097779780626297,
-0.004380224738270044,
0.008615846745669842,
-0.018968109041452408,
0.02974572405219078,
0.027550095692276955,
-0.019503682851791382,
-0.07014442980289459,
-0.02654922753572464,
-0.024047749117016792,
-0.06472909450531006,
0.06479519605636597,
0.018769821152091026,
0.06109610199928284,
-0.008546680212020874,
0.029270946979522705,
0.03105643391609192,
0.011491583660244942,
-0.021754588931798935,
0.07166897505521774,
-0.02654188685119152,
-0.02447563409805298,
0.024877673014998436,
0.026198415085673332,
0.07610590010881424,
-0.02101733349263668,
-0.03160293400287628,
-0.06977437436580658,
-0.022197457030415535,
0.1061004102230072,
0.02973262406885624,
0.02510467916727066,
-0.016823820769786835,
-0.03894176334142685,
-0.03277502581477165,
0.003469520714133978,
0.02018827572464943,
0.03759443387389183,
0.03359444811940193,
0.008710263296961784,
-0.01863647624850273,
0.009108349680900574,
-0.0036961091682314873,
-0.03715189918875694,
-0.008228686638176441,
0.027222849428653717,
0.08004361391067505,
0.06098485738039017,
0.0003134133294224739,
0.041951168328523636,
-0.044299643486738205,
-0.009020242840051651,
-0.04211655631661415,
0.003713606158271432,
0.063290536403656,
-0.014697748236358166,
-0.006767777260392904,
-0.038570649921894073,
-0.0966937392950058,
0.032152991741895676,
-0.08264735341072083,
-0.034145575016736984,
-0.04280932620167732,
0.02070409618318081,
-0.0013740707654505968,
-0.03944464027881622,
0.047007687389850616,
-0.030956823378801346,
0.02023574709892273,
0.0456969290971756,
-0.015420769341289997,
-0.005694324616342783,
-0.07216551154851913,
-0.012091627344489098,
-0.04858715832233429,
-0.06044269725680351,
0.0032398183830082417,
0.02475171536207199,
0.08728303015232086,
0.03248501941561699,
-0.038444884121418,
-0.0069020697847008705,
-0.045276202261447906,
0.0130500178784132,
0.022008948028087616,
-0.01620090939104557,
0.03469472378492355,
0.024357086047530174,
-0.016906004399061203,
-0.2761695086956024,
-0.0749923586845398,
-0.04016536474227905,
-0.04417313262820244,
-0.03820742294192314,
-0.0012974540004506707,
-0.024419685825705528,
-0.008310365490615368,
0.0029753560665994883,
0.006908367853611708,
-0.005577781703323126,
0.08445730805397034,
-0.05258195474743843,
-0.053786054253578186,
0.0242641381919384,
0.06293665617704391,
-0.0019187384750694036,
-0.03221207484602928,
-0.05391331762075424,
-0.03183387964963913,
-0.025553999468684196,
0.004108167719095945,
0.0070790559984743595,
0.01744304783642292,
0.062260180711746216,
-0.027536140754818916,
0.15170498192310333,
0.0033665907103568316,
-0.018105145543813705,
-0.08369559049606323,
0.01762179285287857,
0.05463579297065735,
-0.08045949041843414,
-0.0293782539665699,
0.045494526624679565,
-0.02891681343317032,
0.010659432038664818,
-0.022291436791419983,
-0.016999168321490288,
-0.03885583579540253,
-0.10462065786123276,
-0.014258772134780884,
-0.03501882031559944,
0.035595521330833435,
-0.026396511122584343,
-0.03764090687036514,
-0.009406388737261295,
0.06044912338256836,
0.019481485709547997,
0.04821931943297386,
0.0183352492749691,
-0.03807532787322998,
0.0019525694660842419,
0.030219465494155884,
0.10528463125228882,
-0.03989613428711891,
-0.032611265778541565,
-0.02079113759100437,
-0.06022480130195618,
-0.011592116206884384,
-0.011170859448611736,
-0.014890342019498348,
0.0399884395301342,
-0.061885248869657516,
0.016133133322000504,
0.0011666450882330537,
0.0354185551404953,
0.015597723424434662,
0.08731743693351746,
-0.04335569590330124,
-0.018076004460453987,
0.0801534354686737,
0.03420728072524071,
-0.055972181260585785,
0.1326136738061905,
0.00017235337872989476,
0.04215747490525246,
-0.02719080075621605,
0.02719816006720066,
0.013209854252636433,
0.0392676442861557,
-0.0940488651394844,
0.029400553554296494,
0.014226008206605911,
-0.013470818288624287,
0.02180456556379795,
-0.01805826649069786,
0.024362770840525627,
-0.044468801468610764,
-0.03193676844239235,
0.012544343248009682,
0.023158226162195206,
-0.03344044089317322,
0.03511884808540344,
0.008479277603328228,
-0.029914097860455513,
-0.28836214542388916,
0.01033677626401186,
0.05307059735059738,
-0.06771599501371384,
-0.008343092165887356,
-0.008592040278017521,
-0.01952512189745903,
-0.03306744620203972,
-0.049887675791978836,
-0.0041194818913936615,
0.06482098996639252,
-0.022253241389989853,
0.03281611576676369,
0.0245856661349535,
-0.08473262190818787,
0.010016429238021374,
0.07017160952091217,
0.008455201052129269,
0.012806445360183716,
-0.066196970641613,
0.013719393871724606,
-0.025265242904424667,
0.12642598152160645,
-0.002926819957792759,
-0.04571625217795372,
0.03370668739080429,
0.017489179968833923,
0.05154380947351456,
-0.06329506635665894,
0.014946289360523224,
-0.0003513700794428587,
-0.030142564326524734,
0.012502390891313553,
0.03379584103822708,
0.026631159707903862,
0.002952161943539977,
0.01712849549949169,
0.08791923522949219,
0.02038336917757988,
0.03340177610516548,
0.027687251567840576,
-0.04118697717785835,
-0.04229745268821716,
-0.06081157177686691,
-0.006310363300144672,
-0.018487541005015373,
-0.04143983870744705,
0.025759117677807808,
0.03282520920038223,
0.043067120015621185,
0.013523576781153679,
-0.0555676594376564,
0.045446090400218964,
0.013643484562635422,
0.03518489748239517,
-0.0014971754280850291,
0.00863972119987011,
-0.0511089526116848,
0.07817640155553818,
-0.002651755465194583,
0.027158863842487335,
-0.012993994168937206,
-0.09182101488113403,
0.0584399588406086,
0.046202629804611206
] |
PMID:27158 | Experiments on the preparations and testing of associated vaccine against foot and mouth disease and vesicular disease in swine. | Tests for associated immunization of swine against Foot and Mouth Disease (FMD) and Vesicular Disease (SVD) of swine were carried out. As a result of this investigation, it was established that the prepared and tested inactivated oil vaccine is harmless and immunogenic in sensitive animals. In investigating the course of immunity, the presence of antibody against both antigens was demonstrated in vaccinated animals. All once-vaccinated animals were defended against the virus of SVD during challenge, and 75% of them were defended against FMD. After revaccination, all immunized swine were defended against infection with both viruses. The question of the quality of the associated vaccine and the possibilities of its massive use in industrial swine rearing was discussed. | Experiments on the preparations and testing of associated vaccine against foot and mouth disease and vesicular disease in swine. Tests for associated immunization of swine against Foot and Mouth Disease (FMD) and Vesicular Disease (SVD) of swine were carried out. As a result of this investigation, it was established that the prepared and tested inactivated oil vaccine is harmless and immunogenic in sensitive animals. In investigating the course of immunity, the presence of antibody against both antigens was demonstrated in vaccinated animals. All once-vaccinated animals were defended against the virus of SVD during challenge, and 75% of them were defended against FMD. After revaccination, all immunized swine were defended against infection with both viruses. The question of the quality of the associated vaccine and the possibilities of its massive use in industrial swine rearing was discussed. | [
-0.027108168229460716,
0.019203249365091324,
0.008911137469112873,
0.0147922458127141,
0.04450935870409012,
0.007808445952832699,
0.03574556112289429,
0.07500690221786499,
-0.047822196036577225,
-0.028900884091854095,
-0.013264091685414314,
-0.12093785405158997,
0.06928785145282745,
-0.06260789185762405,
-0.009588982909917831,
-0.03394431620836258,
0.01503053866326809,
-0.037965551018714905,
-0.05175275355577469,
0.036206502467393875,
0.005655200686305761,
-0.004987957887351513,
0.01530168391764164,
-0.10844316333532333,
0.02643638476729393,
0.07521077245473862,
0.027858655899763107,
0.039065901190042496,
-0.021149693056941032,
-0.1612362414598465,
0.009935792535543442,
-0.0804804265499115,
-0.042781513184309006,
-0.035346176475286484,
-0.06277091056108475,
-0.021534541621804237,
-0.007255985401570797,
0.001666148193180561,
-0.05506885424256325,
0.03865522891283035,
0.11482606083154678,
0.015743229538202286,
-0.024554558098316193,
-0.029971826821565628,
0.006664457730948925,
-0.010741176083683968,
-0.08109383285045624,
-0.015797629952430725,
0.05629678815603256,
0.01575653627514839,
0.013657196424901485,
0.0002364686515647918,
0.039879754185676575,
-0.0063415877521038055,
0.012030868791043758,
-0.0074487305246293545,
-0.040486883372068405,
-0.027242645621299744,
-0.011893785558640957,
0.06760599464178085,
-0.011553575284779072,
0.054289642721414566,
-0.13756386935710907,
0.07727769017219543,
0.017766492441296577,
-0.007679218892008066,
-0.029124639928340912,
-0.04204754903912544,
0.021035708487033844,
0.015835823491215706,
-0.021553775295615196,
0.024589085951447487,
0.004654475022107363,
0.029190711677074432,
-0.04497942328453064,
0.035613104701042175,
0.0014193517854437232,
0.018533730879426003,
-0.00006101192775531672,
-0.03507800027728081,
-0.018407531082630157,
0.025711286813020706,
0.020771794021129608,
-0.010209369473159313,
-0.045151278376579285,
-0.042665738612413406,
0.031379710882902145,
-0.06606128811836243,
0.057583037763834,
0.06205969303846359,
0.007526068016886711,
0.008241049014031887,
-0.003424789058044553,
-0.022373046725988388,
-0.02669016644358635,
0.008961059153079987,
0.045509181916713715,
-0.022266266867518425,
-0.013853861950337887,
0.3825955390930176,
-0.03003833070397377,
-0.0243983156979084,
-0.04411124810576439,
-0.07224199175834656,
-0.008994785137474537,
0.005788404028862715,
0.00801382027566433,
-0.013065665028989315,
0.014272701926529408,
0.04775096848607063,
0.031236441805958748,
0.04047638177871704,
-0.02529473416507244,
-0.003078003181144595,
-0.03859958425164223,
-0.005165002774447203,
0.07115545868873596,
0.0034625506959855556,
-0.01365400105714798,
0.0539688766002655,
0.0046882289461791515,
-0.03193823993206024,
0.022020423784852028,
-0.06283153593540192,
0.026860162615776062,
0.008515886962413788,
0.051014695316553116,
0.10009846091270447,
-0.028919169679284096,
-0.02655978687107563,
-0.014062874019145966,
-0.05361662060022354,
0.029100507497787476,
-0.024893373250961304,
0.012052168138325214,
0.005912136286497116,
-0.017479443922638893,
0.011020765639841557,
0.018323589116334915,
0.03902469947934151,
-0.04300867021083832,
-0.05751751363277435,
0.02407391369342804,
-0.06518876552581787,
-0.025310879573225975,
0.03926529362797737,
-0.0011041307589039207,
0.006692833732813597,
0.02263806015253067,
0.03054012358188629,
0.027312666177749634,
0.04578198492527008,
0.01218964159488678,
0.06581635773181915,
-0.032272785902023315,
0.0047595566138625145,
0.007158584892749786,
0.02062723971903324,
-0.04143838211894035,
-0.030214019119739532,
-0.05282865837216377,
-0.08527052402496338,
-0.02823948860168457,
0.058302007615566254,
0.023433011025190353,
-0.04132848605513573,
-0.02775503322482109,
-0.014054341241717339,
0.023683758452534676,
-0.029677782207727432,
0.03652016073465347,
-0.025511709973216057,
-0.005803186446428299,
-0.005443644244223833,
-0.04865849390625954,
-0.04309174418449402,
-0.08387459069490433,
0.002840536180883646,
-0.014628124423325062,
0.026086460798978806,
-0.004851670935750008,
-0.04160120338201523,
0.001635091844946146,
0.014450927264988422,
0.01608116365969181,
0.023802876472473145,
-0.07093218713998795,
0.06040295213460922,
-0.04435299336910248,
0.030499137938022614,
0.027070827782154083,
-0.024016644805669785,
-0.08818963170051575,
-0.028304092586040497,
0.004275478422641754,
0.0473901741206646,
-0.019353056326508522,
0.015298756770789623,
0.04613899439573288,
-0.0530163012444973,
0.028906039893627167,
-0.05068660527467728,
-0.008167827501893044,
0.006527176592499018,
0.01667095348238945,
0.01318600308150053,
0.02992328256368637,
-0.0480327233672142,
0.021164624020457268,
-0.03618822991847992,
-0.04619370773434639,
-0.010678931139409542,
-0.050553251057863235,
-0.03796683996915817,
-0.056966423988342285,
0.0371268056333065,
-0.008170101791620255,
-0.01391650177538395,
0.04478510096669197,
0.029840417206287384,
0.09036867320537567,
0.05624466389417648,
0.007791415322571993,
-0.30861973762512207,
-0.10568314045667648,
0.01160022895783186,
-0.04937664791941643,
0.030942706391215324,
-0.04151728376746178,
-0.004061466548591852,
-0.02536424808204174,
0.03908057510852814,
-0.024935681372880936,
0.05098073557019234,
0.032546110451221466,
-0.04451007768511772,
0.0373806469142437,
0.03057044930756092,
0.049873236566782,
0.06885487586259842,
-0.0443648099899292,
-0.03229084238409996,
0.019241783767938614,
-0.00792558304965496,
-0.02990390546619892,
0.08294674009084702,
0.03638772666454315,
0.08392132073640823,
-0.03849402070045471,
0.10743305087089539,
0.04651430994272232,
0.014651084318757057,
-0.05021537467837334,
-0.028292886912822723,
0.07651146501302719,
-0.025716781616210938,
-0.08226919174194336,
-0.017396768555045128,
0.0038400953635573387,
-0.06090464070439339,
0.0284513458609581,
0.005991844460368156,
-0.0257992222905159,
-0.018155306577682495,
0.0057966201566159725,
-0.007448158226907253,
-0.05215618014335632,
0.019266463816165924,
-0.0015019775601103902,
-0.03334099054336548,
0.03480171039700508,
-0.012432684190571308,
0.020766014233231544,
0.043828271329402924,
-0.03169844672083855,
0.04756865277886391,
0.10467948019504547,
0.00791870430111885,
0.011900398880243301,
-0.024158839136362076,
-0.004476786591112614,
-0.021455466747283936,
0.003747864393517375,
0.05491986125707626,
0.029602091759443283,
0.026475856080651283,
-0.041372690349817276,
-0.05322937294840813,
0.00570117449387908,
0.011375626549124718,
-0.020576227456331253,
0.032196640968322754,
0.028570886701345444,
-0.04222670942544937,
0.08347571641206741,
0.05996770039200783,
0.001277472823858261,
0.023131689056754112,
0.06344778090715408,
-0.002705958439037204,
-0.059425272047519684,
-0.03749953955411911,
0.06898988038301468,
0.06377463787794113,
-0.04935586825013161,
0.028408953920006752,
0.044531531631946564,
0.028909582644701004,
0.033752959221601486,
-0.011257557198405266,
0.04105532914400101,
0.05130958557128906,
0.0030928815249353647,
0.02952614426612854,
-0.05321650952100754,
-0.006918380036950111,
-0.026158303022384644,
-0.056457143276929855,
-0.013295751065015793,
-0.2682277262210846,
-0.006128658074885607,
0.02467484585940838,
0.05120031163096428,
-0.0470416359603405,
-0.010450080037117004,
-0.012553861364722252,
-0.042536452412605286,
-0.0034935385920107365,
-0.04030734300613403,
0.05079509690403938,
0.030319446697831154,
0.08841098845005035,
0.019544947892427444,
0.008326725102961063,
0.04681647568941116,
0.012751612812280655,
-0.03500079736113548,
0.040578048676252365,
-0.07904423773288727,
-0.014219474047422409,
-0.03239463269710541,
0.15628038346767426,
0.03826237842440605,
-0.0683339387178421,
-0.00521700968965888,
0.01921568438410759,
0.0150652090087533,
-0.038129039108753204,
-0.009288521483540535,
0.07002237439155579,
-0.04196720942854881,
0.06181515008211136,
0.04317302256822586,
0.0033712496515363455,
0.03439050167798996,
-0.014390925876796246,
0.013482079841196537,
-0.02295544743537903,
-0.01987263932824135,
0.005325876642018557,
0.030741402879357338,
-0.0002565515460446477,
-0.04879278317093849,
0.013352565467357635,
-0.04945937544107437,
0.017839903011918068,
-0.021374402567744255,
0.0068464805372059345,
-0.021290691569447517,
0.061437856405973434,
-0.05802561715245247,
0.02900221012532711,
0.010615102015435696,
0.00894192699342966,
-0.04297642782330513,
0.0768992006778717,
0.055224597454071045,
-0.019811123609542847,
0.061381466686725616,
0.03710462525486946,
-0.02950756810605526,
-0.05244404822587967,
0.09217244386672974,
0.053438350558280945
] |
PMID:27159 | [Properties and isozymes of acid and alkaline phosphatase in cattle testes and endometrium]. | The activity of acid phosphatase in the testicular parenchyma of cattle was found to be higher than that in the endometrium. The acid phosphatase of either tissue broke up p-nitrophenylphosphate at a rate twice as high as that reached for di-sodium phenylphosphate. Hydrolysis of phenolphthalein diphosphate was slower and that of beta-glycerophosphate very slow. Two optimum pH values and two isozymes were recordable from acid phosphatase in testicular supernatant and three optimum pH values as well as three isozymes from that in endometrial supernatant. Even concentrations pf 0.2 mM of copper and mercury ions were strongly inhibiting the acid phosphatase, whereas lead and cadmium ions proved less effective. The activity of acid phosphatase from testes dropped by 24 per cent and that from the endometrium by 16 per cent over eight weeks of storage at -20 degrees C. The average activity of alkaline phosphatase in the endometrium was much higher than that in testicular parenchyma. Alkaline phosphatase from testes exhibited the shortest break-up time for p-nitrophenylphosphate, whereas disodium-phenylphosphate was broken up at the highest rate by endometrial alkaline phosphatase. Two isozymes of alkaline phosphatase were recordable each from the testes and endometrium. | [Properties and isozymes of acid and alkaline phosphatase in cattle testes and endometrium]. The activity of acid phosphatase in the testicular parenchyma of cattle was found to be higher than that in the endometrium. The acid phosphatase of either tissue broke up p-nitrophenylphosphate at a rate twice as high as that reached for di-sodium phenylphosphate. Hydrolysis of phenolphthalein diphosphate was slower and that of beta-glycerophosphate very slow. Two optimum pH values and two isozymes were recordable from acid phosphatase in testicular supernatant and three optimum pH values as well as three isozymes from that in endometrial supernatant. Even concentrations pf 0.2 mM of copper and mercury ions were strongly inhibiting the acid phosphatase, whereas lead and cadmium ions proved less effective. The activity of acid phosphatase from testes dropped by 24 per cent and that from the endometrium by 16 per cent over eight weeks of storage at -20 degrees C. The average activity of alkaline phosphatase in the endometrium was much higher than that in testicular parenchyma. Alkaline phosphatase from testes exhibited the shortest break-up time for p-nitrophenylphosphate, whereas disodium-phenylphosphate was broken up at the highest rate by endometrial alkaline phosphatase. Two isozymes of alkaline phosphatase were recordable each from the testes and endometrium. | [
0.012806457467377186,
0.03933039307594299,
0.004220582079142332,
0.04455618932843208,
0.03523236885666847,
-0.04703262075781822,
0.043408673256635666,
0.0628315731883049,
-0.028765210881829262,
-0.032565370202064514,
0.013300073333084583,
-0.12071303278207779,
0.031185027211904526,
0.03621089085936546,
0.008615637198090553,
-0.022478366270661354,
-0.013189916498959064,
-0.011405082419514656,
-0.06939021497964859,
0.025574419647455215,
0.0496758334338665,
0.06639935821294785,
0.012215505354106426,
-0.030263513326644897,
0.0166120994836092,
0.0368800163269043,
0.026176298037171364,
0.028378987684845924,
-0.06492258608341217,
-0.15704916417598724,
-0.015973327681422234,
0.000780751695856452,
-0.003988976124674082,
-0.04666759818792343,
-0.015431155450642109,
-0.00492521608248353,
0.021635478362441063,
-0.011187651194632053,
-0.010547791607677937,
0.058974310755729675,
0.06934721022844315,
-0.007271216716617346,
-0.013128540478646755,
0.03710835427045822,
-0.0001874781446531415,
0.04531441628932953,
-0.018482113257050514,
0.00037332004285417497,
0.11758806556463242,
0.004996619187295437,
-0.031668275594711304,
0.03054180182516575,
-0.004204312339425087,
0.006724918261170387,
0.016969263553619385,
-0.04439697787165642,
0.034659791737794876,
0.05089655518531799,
-0.01689630001783371,
-0.005482582841068506,
0.009977703914046288,
-0.0001143818735727109,
-0.14723241329193115,
0.0398457907140255,
0.03801757097244263,
-0.010963852517306805,
-0.029231641441583633,
-0.04283827543258667,
0.050950437784194946,
0.07354649901390076,
-0.04234369844198227,
0.07655245065689087,
0.02643227018415928,
-0.043797314167022705,
0.014483930543065071,
-0.009307452477514744,
-0.012891160324215889,
-0.014271320775151253,
0.02896747551858425,
0.005518837831914425,
-0.07167461514472961,
0.003135665785521269,
0.01184014417231083,
0.04019749164581299,
-0.017878729850053787,
-0.03216199949383736,
-0.011375349946320057,
0.027951186522841454,
0.01784852147102356,
-0.0002823158574756235,
0.0022128685377538204,
-0.04749015346169472,
-0.04189378768205643,
-0.03303135558962822,
-0.01792212203145027,
0.09998176246881485,
0.013331674039363861,
-0.012716242112219334,
-0.010555208660662174,
0.3958396911621094,
-0.0934678390622139,
0.0014460908714681864,
-0.06249862164258957,
-0.10061697661876678,
-0.014318553730845451,
0.00630825012922287,
-0.02919759787619114,
-0.023254621773958206,
-0.034414321184158325,
0.08476586639881134,
0.06727038323879242,
0.008289625868201256,
0.036146145313978195,
-0.05935246869921684,
-0.018665825948119164,
-0.060640253126621246,
0.024891676381230354,
0.028647195547819138,
-0.029180945828557014,
0.02802208997309208,
-0.008687210269272327,
0.012662391178309917,
0.017833445221185684,
-0.055231861770153046,
0.0638212338089943,
0.006923881359398365,
0.033915333449840546,
0.06420467048883438,
0.03150787204504013,
0.005861999932676554,
0.001782837207429111,
0.021346965804696083,
-0.02975648082792759,
0.0224628746509552,
0.0029704661574214697,
-0.049515750259160995,
0.05423760414123535,
-0.005564212799072266,
0.029549289494752884,
-0.026350224390625954,
-0.03838565573096275,
-0.11109235137701035,
-0.05112598463892937,
-0.05830211564898491,
0.0034533061552792788,
0.11764239519834518,
0.008507386781275272,
0.01717747189104557,
-0.009378056041896343,
-0.026996007189154625,
0.046022962778806686,
0.06568197160959244,
0.014768504537642002,
0.0534512922167778,
-0.03604511171579361,
-0.04570003226399422,
-0.0060163880698382854,
-0.0179857537150383,
0.020468566566705704,
-0.043597716838121414,
-0.04261668026447296,
-0.0001858151808846742,
0.017582491040229797,
0.015345371328294277,
0.0717417374253273,
0.010121047496795654,
-0.02368219569325447,
-0.029120856896042824,
-0.05732545256614685,
-0.057107243686914444,
-0.000824534974526614,
-0.010341373272240162,
-0.011700894683599472,
-0.07298657298088074,
-0.032182320952415466,
-0.021116215735673904,
0.03685746714472771,
0.00710566621273756,
0.03385646268725395,
-0.012387885712087154,
0.03738723695278168,
-0.03705591708421707,
-0.06053467094898224,
0.05401686206459999,
0.019221050664782524,
-0.052305251359939575,
-0.03803863003849983,
-0.004884323105216026,
0.01587766408920288,
0.04134990647435188,
0.029969386756420135,
0.00016331917140632868,
-0.024346711114048958,
-0.02063046768307686,
-0.047003332525491714,
-0.0746259018778801,
-0.012437784112989902,
0.010164786130189896,
0.025095079094171524,
-0.046902719885110855,
0.07806901633739471,
0.02426774986088276,
0.004711433779448271,
0.06191885098814964,
-0.03176257014274597,
0.08265509456396103,
-0.05506386235356331,
0.04229194298386574,
0.059051018208265305,
0.021754741668701172,
-0.018674802035093307,
-0.03323943167924881,
-0.056757375597953796,
0.006804642733186483,
-0.079995296895504,
0.027948293834924698,
0.012202241457998753,
-0.061609238386154175,
0.045937057584524155,
-0.00004356791760073975,
0.038862764835357666,
-0.03318050503730774,
-0.018751297146081924,
-0.2893785536289215,
-0.05996140465140343,
0.000020575605958583765,
0.02944285422563553,
0.06518196314573288,
-0.032821256667375565,
0.011395721696317196,
-0.022913102060556412,
0.029033876955509186,
-0.05146583914756775,
0.04085240885615349,
0.03351327031850815,
-0.008843163028359413,
0.04284285753965378,
0.02776380255818367,
0.03959880769252777,
0.004191751126199961,
-0.058747585862874985,
0.03024163842201233,
0.014099775813519955,
0.01939643919467926,
-0.031306639313697815,
0.028523307293653488,
0.02916235476732254,
0.08510739356279373,
-0.024881606921553612,
0.09549164026975632,
-0.008267570286989212,
0.017802275717258453,
-0.045174114406108856,
0.05207144841551781,
0.05191909894347191,
0.006537618115544319,
-0.06171751394867897,
-0.024143505841493607,
-0.026051511988043785,
0.006369763519614935,
-0.03238259255886078,
-0.019914690405130386,
-0.0022011995315551758,
-0.04148716479539871,
-0.031043754890561104,
-0.04176899418234825,
-0.053773485124111176,
0.0028587121050804853,
0.03347969800233841,
0.01988700032234192,
0.011054032482206821,
0.035750944167375565,
-0.018919751048088074,
0.02719864994287491,
-0.0009098305599763989,
0.03828420862555504,
-0.06737120449542999,
-0.027722002938389778,
-0.009317770600318909,
-0.020325861871242523,
0.02982369065284729,
-0.0697622150182724,
-0.012027503922581673,
-0.015541983768343925,
-0.0355089046061039,
-0.00560194393619895,
0.02601649984717369,
-0.01439651194959879,
-0.06374252587556839,
0.0030498765408992767,
-0.001132094650529325,
0.016222910955548286,
0.016775663942098618,
0.02735484205186367,
0.08482850342988968,
-0.061723239719867706,
-0.009733723476529121,
0.041287630796432495,
-0.0025000469759106636,
-0.06066226586699486,
0.02571546845138073,
-0.04646195098757744,
-0.0100944172590971,
0.03087957389652729,
-0.034474506974220276,
0.0605127289891243,
-0.0464443638920784,
-0.0032288390211760998,
0.07761841267347336,
0.031302474439144135,
-0.028125746175646782,
0.07362206280231476,
0.0298644807189703,
0.03465668857097626,
-0.025167694315314293,
-0.0667307898402214,
-0.004210157319903374,
0.0533088780939579,
0.014974942430853844,
-0.28024813532829285,
-0.010990467853844166,
-0.000017180476788780652,
-0.018675146624445915,
-0.06137625873088837,
0.06010999158024788,
0.03760220482945442,
-0.020615622401237488,
0.008895591832697392,
0.06140795722603798,
0.03937589377164841,
0.04494941979646683,
0.04685066640377045,
-0.0223802849650383,
-0.03438907116651535,
0.02229556441307068,
-0.008376965299248695,
0.01915021985769272,
0.031674519181251526,
-0.02302890457212925,
0.00889905821532011,
-0.06843283772468567,
0.11677823215723038,
-0.006835373118519783,
0.055034056305885315,
0.06916886568069458,
-0.00617016339674592,
0.0066272118128836155,
-0.009455992840230465,
-0.0132663669064641,
0.01329509075731039,
0.04032379761338234,
0.046483203768730164,
-0.042319152504205704,
0.0029282865580171347,
0.08429878205060959,
-0.004382181912660599,
-0.00506775826215744,
-0.02019120566546917,
-0.03716418892145157,
0.022243473678827286,
-0.0246293768286705,
-0.09146934747695923,
-0.011362147517502308,
0.025938114151358604,
-0.10747988522052765,
-0.04224860295653343,
-0.034523677080869675,
0.011879651807248592,
-0.018398407846689224,
-0.006506679579615593,
-0.05082996189594269,
0.03242874518036842,
0.08934391289949417,
-0.005187434609979391,
-0.007425894029438496,
-0.02166029065847397,
-0.017298204824328423,
-0.005200296174734831,
0.001633679959923029,
0.055914491415023804,
-0.008237000554800034,
-0.028354566544294357,
0.11295906454324722,
0.06997306644916534
] |
PMID:27160 | [Relationship between the limbic system and gonadal function. 3. Quantifiable prepuberal morphokinesis of the basolateral amygdaloid body and the ventral hippocampus formation]. | In juvenile female rats the neurons of the limbic system which are attached to the basolateral amygdaloid nucleus and to a circumscribed area of ventral hippocampal formation are characterised by markedly quantifiable morphokinesis between the 21st day of age and onset of the first oestrus. This morphokinesis will take the form of consecutively occurring highly significant volume alterations of the cell nuclei which, in turn, reflect variation in neuronal activity. Some of such variation represents a expression of the general maturation and differentiation of central nervous neurons. The time of occurrence of such variation as well as its peculiar nature also appear to suggest the existence of some special relationship to sexual maturation. This seems to support the conclusion that the basolateral amygdaloid nucleus and the ventral hippocampal formation are attached to the extrahypothalamic central nervous structures of the cerebro-hypophyseal-gonadal axis, in addition to the medial and cortical amygdaloid nuclei. However, the very nature of relationships that may exist between the two investigated components of the limbic system, on the one hand, and sexual maturation as well as gonadal function, on the other, cannot be derived from the results reported in this paper. | [Relationship between the limbic system and gonadal function. 3. Quantifiable prepuberal morphokinesis of the basolateral amygdaloid body and the ventral hippocampus formation]. In juvenile female rats the neurons of the limbic system which are attached to the basolateral amygdaloid nucleus and to a circumscribed area of ventral hippocampal formation are characterised by markedly quantifiable morphokinesis between the 21st day of age and onset of the first oestrus. This morphokinesis will take the form of consecutively occurring highly significant volume alterations of the cell nuclei which, in turn, reflect variation in neuronal activity. Some of such variation represents a expression of the general maturation and differentiation of central nervous neurons. The time of occurrence of such variation as well as its peculiar nature also appear to suggest the existence of some special relationship to sexual maturation. This seems to support the conclusion that the basolateral amygdaloid nucleus and the ventral hippocampal formation are attached to the extrahypothalamic central nervous structures of the cerebro-hypophyseal-gonadal axis, in addition to the medial and cortical amygdaloid nuclei. However, the very nature of relationships that may exist between the two investigated components of the limbic system, on the one hand, and sexual maturation as well as gonadal function, on the other, cannot be derived from the results reported in this paper. | [
0.044386059045791626,
-0.004364998545497656,
0.02108059450984001,
-0.0033346442505717278,
-0.0005091818165965378,
0.08212904632091522,
0.018673015758395195,
0.010945647023618221,
0.026943793520331383,
-0.02687421441078186,
0.0012092894176021218,
-0.06999234855175018,
0.07402019202709198,
0.041737351566553116,
-0.014656279236078262,
0.020473118871450424,
-0.027201974764466286,
-0.0034286181908100843,
-0.010958805680274963,
0.028845202177762985,
0.04487038031220436,
-0.05352696403861046,
0.016202930361032486,
0.0032824166119098663,
-0.0005050002946518362,
-0.04332961514592171,
-0.030963683500885963,
0.006907068658620119,
-0.03551138564944267,
-0.17591935396194458,
0.03053566813468933,
0.03565523773431778,
-0.024826278910040855,
-0.06974979490041733,
-0.004255964420735836,
-0.006411357782781124,
0.03286507725715637,
0.014785652048885822,
-0.024236734956502914,
0.0020614666864275932,
0.0398264043033123,
0.020320238545536995,
-0.018195195123553276,
-0.027721399441361427,
0.04548315331339836,
0.005652341526001692,
-0.05582602322101593,
-0.01005600392818451,
0.03333815187215805,
-0.00831134058535099,
-0.03358186408877373,
-0.028097936883568764,
0.030060026794672012,
0.09491585940122604,
0.010087361559271812,
0.0425666980445385,
0.040385205298662186,
0.08001063019037247,
-0.003067265497520566,
0.05144071578979492,
-0.02710723504424095,
0.025562753900885582,
-0.12496175616979599,
0.0439685583114624,
0.04124538600444794,
-0.02590467408299446,
-0.043211039155721664,
-0.06948891282081604,
0.03047119453549385,
0.0027624983340501785,
-0.020641162991523743,
0.07709740847349167,
0.007227869238704443,
-0.0214023869484663,
0.03630948066711426,
-0.01809716783463955,
-0.05322980880737305,
-0.00609917426481843,
0.05628807842731476,
-0.028351327404379845,
0.02601608820259571,
0.07437822967767715,
0.024131735786795616,
-0.020286794751882553,
-0.012784956954419613,
-0.05864770710468292,
-0.0029345895163714886,
-0.08197133988142014,
-0.057056985795497894,
0.01372493989765644,
-0.08540212363004684,
-0.011146360076963902,
-0.012922786176204681,
-0.027489326894283295,
-0.013920459896326065,
0.061351701617240906,
0.022958386689424515,
-0.03326274827122688,
0.006837446708232164,
0.4190904498100281,
-0.02204044908285141,
0.012581218034029007,
0.028177300468087196,
-0.004729528445750475,
-0.019834080711007118,
0.02019619010388851,
0.009507497772574425,
-0.02687024511396885,
-0.07047833502292633,
0.07346899807453156,
-0.025337567552924156,
-0.024476712569594383,
0.039873622357845306,
-0.04350665584206581,
0.012451828457415104,
-0.09222693741321564,
0.0708971694111824,
-0.01618270017206669,
0.014013157226145267,
-0.04185254126787186,
-0.0397869274020195,
-0.01824132166802883,
0.03832291066646576,
-0.0012537919683381915,
0.09683623909950256,
-0.04399124160408974,
-0.01955675147473812,
0.08338963985443115,
0.06060316041111946,
-0.049142271280288696,
0.10513316839933395,
0.09056147933006287,
0.029272856190800667,
0.02430730313062668,
0.04974396899342537,
-0.020262539386749268,
0.015147262252867222,
-0.024128636345267296,
-0.02276359684765339,
-0.020986435934901237,
-0.006166915874928236,
-0.06257378309965134,
0.07428260147571564,
-0.0627792701125145,
-0.04478463903069496,
0.08988136053085327,
0.0055259158834815025,
-0.03492842987179756,
-0.02508634328842163,
0.1157648116350174,
-0.016310274600982666,
0.00721821840852499,
-0.02762666344642639,
0.0135022709146142,
0.018755881115794182,
0.006371883675456047,
-0.026052789762616158,
0.021644605323672295,
-0.03667626902461052,
-0.006021835841238499,
-0.02477789856493473,
0.01181179378181696,
-0.023713339120149612,
0.038339752703905106,
0.07065322995185852,
0.008948740549385548,
-0.004847962409257889,
0.0050162626430392265,
-0.01846454106271267,
-0.03515837341547012,
0.01748528704047203,
0.01779962331056595,
-0.015307528898119926,
-0.03856296464800835,
-0.03336691856384277,
-0.01522112637758255,
0.02985675446689129,
0.0009195455932058394,
-0.029375925660133362,
0.033668357878923416,
0.019197702407836914,
-0.08386394381523132,
-0.06324657052755356,
0.011004496365785599,
-0.022729916498064995,
-0.038267530500888824,
-0.03456065431237221,
-0.03187961503863335,
-0.010124695487320423,
0.005796497222036123,
-0.006366846151649952,
0.011567529290914536,
-0.007847958244383335,
-0.01284837257117033,
-0.009480303153395653,
-0.03719036653637886,
-0.001955449813976884,
0.008713062852621078,
-0.0320914164185524,
-0.0069172135554254055,
0.07084394991397858,
-0.015457128174602985,
-0.00483448663726449,
0.03830195963382721,
-0.0033533121459186077,
-0.0019038708414882421,
0.038894880563020706,
0.02203071303665638,
0.06572369486093521,
0.048177581280469894,
-0.013440997339785099,
-0.05480530485510826,
0.0017660226440057158,
-0.009019827470183372,
-0.02856658585369587,
0.12500600516796112,
-0.002402564510703087,
-0.036868490278720856,
0.01845797896385193,
-0.0013532559387385845,
0.014959093183279037,
-0.023389555513858795,
0.006040340289473534,
-0.2943473160266876,
-0.03358069434762001,
-0.015030167065560818,
-0.04748879745602608,
0.06124609708786011,
-0.08475162833929062,
0.013390657491981983,
-0.023760493844747543,
0.023694947361946106,
-0.05567356199026108,
0.042583685368299484,
0.06495579332113266,
-0.03319323807954788,
0.006867303512990475,
-0.011808176524937153,
0.051613301038742065,
0.06648223847150803,
-0.041765425354242325,
0.011022857390344143,
0.04446634650230408,
-0.029281480237841606,
-0.021086247637867928,
-0.01777227781713009,
-0.003870701417326927,
0.03939320519566536,
-0.03431393951177597,
0.12836776673793793,
0.02861163765192032,
0.03231601417064667,
-0.021059347316622734,
-0.0203699953854084,
0.033766988664865494,
0.04078267142176628,
-0.09171446412801743,
0.04627186059951782,
-0.02225644327700138,
0.013030105270445347,
0.00849149003624916,
-0.021314671263098717,
-0.0042637428268790245,
-0.06401317566633224,
0.010842476971447468,
0.006332280579954386,
-0.04797700047492981,
-0.08841677755117416,
-0.0028346446342766285,
0.006282865069806576,
-0.052428100258111954,
0.0666925236582756,
0.008614320307970047,
0.01801232434809208,
-0.04037974029779434,
-0.03470028191804886,
-0.015130151063203812,
-0.02554381638765335,
-0.02139071747660637,
-0.02495438978075981,
0.004688167478889227,
-0.05736587941646576,
0.008028107695281506,
0.030472980812191963,
-0.027474980801343918,
-0.02013024128973484,
-0.06720002740621567,
-0.002566505689173937,
0.01933530904352665,
-0.018984487280249596,
-0.041009679436683655,
0.024764562025666237,
-0.01322528813034296,
0.034055475145578384,
0.1214713603258133,
-0.04279812052845955,
0.010038708336651325,
0.04079645127058029,
-0.03139689192175865,
-0.033968277275562286,
-0.020051661878824234,
-0.016838539391756058,
0.009390839375555515,
-0.018322709947824478,
-0.08693042397499084,
0.0321614071726799,
0.016191227361559868,
-0.007416512817144394,
-0.01586076244711876,
-0.010355616919696331,
-0.06611645221710205,
0.08019515872001648,
-0.02088053710758686,
-0.004270946141332388,
0.025345537811517715,
-0.02041924186050892,
-0.05104441940784454,
0.03525232523679733,
0.05650918185710907,
-0.30570122599601746,
0.014313575811684132,
0.025988265872001648,
0.005543896462768316,
0.0262905340641737,
0.007887598127126694,
0.009065004996955395,
-0.026557905599474907,
0.05536229908466339,
0.04493662342429161,
0.04635876789689064,
0.015711147338151932,
0.03698154538869858,
0.04136442765593529,
-0.0574897825717926,
0.03758395463228226,
0.039644766598939896,
0.0009666658588685095,
0.04675959423184395,
-0.06574563682079315,
-0.0001951125159393996,
-0.013549519702792168,
0.13219605386257172,
0.006715784780681133,
0.012092947959899902,
-0.002186724217608571,
-0.017371531575918198,
0.029434578493237495,
0.0031489538960158825,
-0.02458217553794384,
0.006582336965948343,
0.05492870509624481,
0.03709929436445236,
-0.014953942969441414,
0.02511824294924736,
0.06099609658122063,
-0.03669896349310875,
0.007200764957815409,
0.07442936301231384,
-0.030334096401929855,
-0.006724735256284475,
0.024909205734729767,
-0.04614138975739479,
0.0020574494265019894,
0.08370810002088547,
-0.01813548058271408,
-0.023169180378317833,
0.04538455605506897,
-0.01305181160569191,
0.006788311991840601,
-0.014553657732903957,
-0.027504965662956238,
-0.004960446618497372,
0.020332379266619682,
0.0013737898552790284,
-0.006742959376424551,
0.02844633348286152,
-0.06352908909320831,
-0.02347574383020401,
-0.02604348212480545,
0.006021378096193075,
-0.011944650672376156,
-0.011319544166326523,
0.051123302429914474,
0.004855683073401451
] |
PMID:27161 | [Blood acid-base status in fattening pigs in exhausting physical stress of high intensity]. | Reported in this paper are changes in the acid-base state and lactic concentration of blood and muscle samples taken from 28 pigs prior to, during, and after high-intensity exhausting stress applied at ambient temperatures between 17 degrees C and 21 degrees C and at 35 degrees C. Close correlations were found to exist between acid-base states, on the one hand, and lactic acid concentrations, on the other. The lactic acid concentrations reached in blood plasma were between 30 and 40 mHol/1, pH being below 7.0 and BE values being smaller than -22 mHol/1. Close relationships were established between the lactic acid concentration of venous blood plasma, blood pH, and the pH of the musculus longissimus dorsi. Respiratory compensation was of minor importance with ambient temperatures between 17 degrees C and 21 degrees C, but it was of considerable effectiveness at 35 degrees C. | [Blood acid-base status in fattening pigs in exhausting physical stress of high intensity]. Reported in this paper are changes in the acid-base state and lactic concentration of blood and muscle samples taken from 28 pigs prior to, during, and after high-intensity exhausting stress applied at ambient temperatures between 17 degrees C and 21 degrees C and at 35 degrees C. Close correlations were found to exist between acid-base states, on the one hand, and lactic acid concentrations, on the other. The lactic acid concentrations reached in blood plasma were between 30 and 40 mHol/1, pH being below 7.0 and BE values being smaller than -22 mHol/1. Close relationships were established between the lactic acid concentration of venous blood plasma, blood pH, and the pH of the musculus longissimus dorsi. Respiratory compensation was of minor importance with ambient temperatures between 17 degrees C and 21 degrees C, but it was of considerable effectiveness at 35 degrees C. | [
-0.035809047520160675,
0.0667358785867691,
0.045840777456760406,
0.07910683006048203,
0.058793433010578156,
0.003917957656085491,
0.07346978783607483,
0.017089081928133965,
-0.022728970274329185,
-0.02011171169579029,
0.023575622588396072,
-0.12524454295635223,
0.019004937261343002,
0.07050754129886627,
-0.03368813171982765,
-0.013927836902439594,
0.04318176209926605,
0.015711365267634392,
-0.03943878039717674,
-0.003996438346803188,
0.005808381829410791,
0.0025307254400104284,
0.03637329488992691,
-0.010159013792872429,
0.054405566304922104,
0.029023071750998497,
-0.014317926950752735,
0.054280105978250504,
-0.07496257871389389,
-0.18993809819221497,
0.022180916741490364,
-0.023755667731165886,
0.02901829034090042,
-0.03378172218799591,
-0.0423583909869194,
0.027147842571139336,
0.00949117448180914,
-0.018807219341397285,
-0.03264636918902397,
0.06723535060882568,
0.10501822829246521,
0.03699636459350586,
-0.020181255415081978,
0.01797061413526535,
-0.016297167167067528,
0.03816930204629898,
-0.00894047413021326,
0.02785453200340271,
0.09027912467718124,
0.0034804081078618765,
-0.009967588819563389,
-0.02136130817234516,
0.014949335716664791,
0.04499667510390282,
-0.014315680600702763,
-0.015391005203127861,
0.0677281990647316,
0.033544789999723434,
0.011762947775423527,
0.006392013281583786,
-0.03719037026166916,
0.03996112197637558,
-0.1474979668855667,
0.035068169236183167,
0.05018438771367073,
0.02191089652478695,
-0.061354249715805054,
-0.03785908222198486,
0.04613865539431572,
0.03862330690026283,
-0.08600130677223206,
0.03190479055047035,
0.02647412195801735,
-0.007271880283951759,
0.07356052100658417,
-0.010289737023413181,
-0.003797353245317936,
0.012079624459147453,
0.02302660420536995,
-0.03216977417469025,
-0.03270973265171051,
0.023197371512651443,
0.006665128283202648,
-0.021386541426181793,
-0.020823385566473007,
-0.03596143797039986,
0.0056594498455524445,
-0.009783283807337284,
-0.0014782112557440996,
0.025699961930513382,
-0.03171289712190628,
-0.06831861287355423,
-0.03220159560441971,
0.008006499148905277,
-0.026254981756210327,
0.005009934771806002,
0.02088029868900776,
0.018662938848137856,
-0.006180465221405029,
0.4242204427719116,
-0.012156106531620026,
0.02785235270857811,
-0.05040857195854187,
0.0034061206970363855,
0.02044617012143135,
0.033805154263973236,
0.006568897049874067,
0.027893200516700745,
-0.05692516639828682,
0.000609923095908016,
0.03620975464582443,
0.0533565916121006,
0.004158494062721729,
-0.03115997649729252,
0.03231600672006607,
0.00026082340627908707,
0.08815470337867737,
-0.011050637811422348,
0.020472869277000427,
0.03822843357920647,
-0.005191556178033352,
0.01325236912816763,
0.01377787347882986,
-0.015767337754368782,
0.07074686139822006,
-0.012004484422504902,
0.02396450564265251,
0.08319395780563354,
0.03277882561087608,
-0.029097728431224823,
0.0498490184545517,
-0.019963905215263367,
0.024053269997239113,
-0.02977420948445797,
-0.051235005259513855,
-0.05427657067775726,
0.032447271049022675,
-0.007333140354603529,
0.0461076982319355,
0.026753755286335945,
-0.004595947917550802,
-0.03236636891961098,
-0.002048692898824811,
-0.0959034115076065,
-0.06509213149547577,
0.07147587090730667,
0.019358022138476372,
0.005548158660531044,
-0.04162438586354256,
-0.019315378740429878,
-0.0020809939596801996,
0.010783040896058083,
0.022330429404973984,
0.043870240449905396,
0.0019398271106183529,
0.03102031908929348,
-0.054018229246139526,
0.01417509000748396,
0.00034671620232984424,
-0.05884237959980965,
-0.033336296677589417,
-0.07451947778463364,
-0.004785026889294386,
0.03512496501207352,
0.03680133819580078,
0.030080150812864304,
-0.009642702527344227,
-0.04235894978046417,
0.03147343546152115,
-0.007546635810285807,
0.032144106924533844,
-0.03567977249622345,
-0.019346844404935837,
-0.04059193283319473,
-0.0001574199995957315,
0.003187402617186308,
0.005510819144546986,
0.002731608459725976,
0.012477820739150047,
0.023026248440146446,
0.08398373425006866,
0.01073481235653162,
-0.044916749000549316,
0.005120423156768084,
0.014436375349760056,
-0.03141136094927788,
-0.10485241562128067,
-0.01424944493919611,
-0.03855236619710922,
0.05379337817430496,
-0.0443735346198082,
-0.038893524557352066,
-0.06275960803031921,
-0.021145358681678772,
0.0019104054663330317,
0.04294133186340332,
-0.010092628188431263,
0.06099050119519234,
0.0031713764183223248,
0.038830000907182693,
0.0733698233962059,
-0.045351363718509674,
0.006428914610296488,
0.027341656386852264,
0.06760289520025253,
0.018480367958545685,
-0.005473529454320669,
-0.01996648497879505,
0.013981550931930542,
-0.039832379668951035,
0.024715781211853027,
0.009742324240505695,
-0.039051178842782974,
-0.03761587291955948,
-0.09973306208848953,
0.014384262263774872,
0.036520346999168396,
-0.035612836480140686,
0.03824923560023308,
-0.0011173674138262868,
0.05612421780824661,
-0.07076145708560944,
-0.0033466725144535303,
-0.29536932706832886,
-0.037474483251571655,
0.0478595606982708,
-0.01934465765953064,
0.08115535229444504,
-0.013028214685618877,
0.029955055564641953,
-0.046743880957365036,
0.013921498320996761,
-0.038304660469293594,
0.06292346864938736,
0.0029137716628611088,
-0.05080924928188324,
-0.06684726476669312,
0.029353484511375427,
0.07638763636350632,
0.03629160299897194,
-0.07385073602199554,
0.04227979853749275,
0.013018692843616009,
0.07164229452610016,
-0.014115607365965843,
-0.024663053452968597,
0.006186656653881073,
0.07182104140520096,
0.003929327242076397,
0.10547162592411041,
-0.08322026580572128,
-0.0060424902476370335,
-0.009975451044738293,
-0.026066336780786514,
-0.007923797704279423,
-0.018514029681682587,
-0.06640827655792236,
-0.02614830620586872,
-0.03154135122895241,
-0.024594493210315704,
0.00722344359382987,
0.030679523944854736,
-0.02094023860991001,
-0.038090433925390244,
0.0008034233469516039,
-0.08326426148414612,
-0.03960369899868965,
-0.04163907468318939,
-0.00883850920945406,
0.04007343202829361,
0.017210302874445915,
-0.03983180224895477,
-0.051594335585832596,
0.041175492107868195,
0.03179514780640602,
-0.048615120351314545,
-0.05877572298049927,
0.015393253415822983,
-0.03665059059858322,
-0.04965422675013542,
-0.0016542490338906646,
-0.06729350984096527,
-0.010587082244455814,
0.02707197517156601,
-0.016266804188489914,
0.047687143087387085,
0.0016468703979626298,
-0.0071288105100393295,
0.018018802627921104,
0.048561275005340576,
0.014178416691720486,
0.022715045139193535,
0.012359512969851494,
0.01748848892748356,
0.08231444656848907,
-0.025137700140476227,
-0.023082800209522247,
0.06871550530195236,
-0.027838561683893204,
-0.031757161021232605,
-0.02372969314455986,
-0.06169508770108223,
-0.006660636980086565,
0.05454658344388008,
-0.05309472605586052,
0.03514980897307396,
-0.004640049301087856,
0.05262375995516777,
0.01590072177350521,
-0.006358663085848093,
0.007657707668840885,
0.054106853902339935,
0.010534432716667652,
-0.007889465428888798,
-0.042497411370277405,
-0.05695375055074692,
-0.01836921088397503,
0.03385920822620392,
0.017673514783382416,
-0.2896316349506378,
0.0034044277854263783,
0.03438512980937958,
0.014505035243928432,
-0.023054005578160286,
-0.02883836440742016,
-0.04007517173886299,
0.020002782344818115,
-0.025157524272799492,
0.024804728105664253,
0.007351808715611696,
0.06951840966939926,
0.02884763479232788,
-0.009020275436341763,
-0.029412485659122467,
0.012884082272648811,
-0.01665029488503933,
-0.002383199520409107,
0.013595557771623135,
0.013075594790279865,
-0.02706265263259411,
-0.08159999549388885,
0.12609632313251495,
0.02848902903497219,
-0.003255727468058467,
0.03997127339243889,
-0.0013878301251679659,
0.022053321823477745,
-0.06236439570784569,
-0.018290992826223373,
0.03526919335126877,
0.005027986131608486,
0.05329188331961632,
-0.030127305537462234,
-0.009066026657819748,
0.015498126856982708,
-0.024855414405465126,
-0.013520671986043453,
-0.005094605032354593,
0.034090179949998856,
-0.006506283767521381,
0.0024938853457570076,
0.015760665759444237,
-0.05840492993593216,
0.057470355182886124,
-0.001522715319879353,
-0.04444026201963425,
-0.05009045824408531,
-0.03051644191145897,
-0.0382206104695797,
-0.04620394855737686,
-0.030312970280647278,
-0.004916958510875702,
0.05878403037786484,
-0.01385822705924511,
0.0020493795163929462,
0.016629667952656746,
-0.010448336601257324,
-0.027968738228082657,
-0.06729738414287567,
0.06750316172838211,
0.04080533608794212,
-0.01950995624065399,
0.05628994107246399,
0.012250451371073723
] |
PMID:27162 | [Studies on diarrhea of young calves. 1. Quantitative analysis of the gastrointestinal flora in clinically healthy calves and in calves with diarrhea]. | Quantitative composition of gastro-intestinal-flora was determined in 23 clinically healthy calves and the course of germ spreading was followed up. In comparison with these healthy animals in 25 diarrhoea affected calves at the age of 4 to 17 days there could be observed signigicant differences only in few parts of the gastro-intestinal-tract concerning the quantitative composition of germ flora. Only in 3 animals enterotoxin producing E coli strains could be detected. | [Studies on diarrhea of young calves. 1. Quantitative analysis of the gastrointestinal flora in clinically healthy calves and in calves with diarrhea]. Quantitative composition of gastro-intestinal-flora was determined in 23 clinically healthy calves and the course of germ spreading was followed up. In comparison with these healthy animals in 25 diarrhoea affected calves at the age of 4 to 17 days there could be observed signigicant differences only in few parts of the gastro-intestinal-tract concerning the quantitative composition of germ flora. Only in 3 animals enterotoxin producing E coli strains could be detected. | [
0.02251581661403179,
0.006388798821717501,
0.06971222907304764,
0.03196560591459274,
0.08921001106500626,
-0.06882651150226593,
-0.015314972959458828,
0.07502877712249756,
-0.026768887415528297,
-0.01343853771686554,
-0.0793994814157486,
-0.11164164543151855,
0.001089901546947658,
-0.029000818729400635,
-0.033957768231630325,
-0.01953498274087906,
-0.0014833691529929638,
-0.05047837644815445,
-0.0712292492389679,
0.014833181165158749,
-0.0006801885319873691,
0.03296705335378647,
0.03136778250336647,
-0.06401125341653824,
0.03806371986865997,
0.015890328213572502,
-0.018026208505034447,
0.001554735004901886,
-0.08329498022794724,
-0.13518962264060974,
-0.006206771824508905,
-0.0073679368942976,
0.06250395625829697,
-0.020823350176215172,
-0.06569253653287888,
0.013677475042641163,
0.027800623327493668,
0.00844571739435196,
-0.021363602951169014,
0.01955365017056465,
-0.0020409803837537766,
0.004217649344354868,
-0.0370958149433136,
0.009483498521149158,
-0.0368921160697937,
-0.04740210995078087,
-0.06231703609228134,
0.004411987494677305,
0.061432432383298874,
-0.026329221203923225,
-0.08390051871538162,
-0.0010237861424684525,
0.02066805772483349,
0.093769870698452,
0.02565017342567444,
0.0002499003894627094,
0.0740879699587822,
-0.015537726692855358,
0.004566684365272522,
0.05892108008265495,
0.0539059080183506,
-0.001275158952921629,
-0.12062002718448639,
0.02364256978034973,
0.01850731670856476,
0.0083857960999012,
-0.02316073514521122,
-0.042133331298828125,
0.013309606350958347,
-0.012098999693989754,
-0.09340912103652954,
0.04362272843718529,
-0.02282501384615898,
0.024394378066062927,
-0.026929719373583794,
-0.03335966542363167,
0.01420697383582592,
-0.032686542719602585,
0.020838266238570213,
0.04530344530940056,
-0.01470771711319685,
0.07477863132953644,
0.0313442088663578,
-0.012241814285516739,
-0.05791156738996506,
0.01567067578434944,
-0.036629531532526016,
0.028006738051772118,
-0.0004259795823600143,
0.05955784022808075,
0.03928019478917122,
-0.08425363153219223,
-0.04702147841453552,
0.01952032372355461,
-0.007930725812911987,
-0.028361234813928604,
0.02006780542433262,
-0.10850059986114502,
0.030651280656456947,
0.3762270510196686,
-0.07101061940193176,
-0.044181402772665024,
0.007475106976926327,
-0.026849595829844475,
0.007172619458287954,
0.05296981334686279,
0.04477457329630852,
0.003548643086105585,
-0.013511431403458118,
0.03742843121290207,
0.005024540703743696,
0.0002415070339338854,
0.03669798746705055,
-0.025616221129894257,
-0.06689496338367462,
-0.045348357409238815,
0.03051675856113434,
0.04311748221516609,
-0.02047525718808174,
0.06589297205209732,
0.041797004640102386,
-0.010726493783295155,
0.006548536941409111,
-0.05483214557170868,
0.09298982471227646,
-0.01758899725973606,
-0.01521499827504158,
0.03696950152516365,
-0.045761652290821075,
-0.039192233234643936,
-0.04397856071591377,
-0.007210637908428907,
-0.013561052270233631,
-0.014459749683737755,
0.002436467446386814,
-0.024468135088682175,
0.04845641180872917,
0.029136372730135918,
0.03213592246174812,
-0.02900121361017227,
-0.014790719375014305,
-0.08285371214151382,
-0.05619494989514351,
-0.10946996510028839,
-0.03902915120124817,
0.0858929306268692,
-0.03501869738101959,
0.02245112881064415,
-0.0706225261092186,
-0.05278085544705391,
0.02853214740753174,
0.05166124179959297,
0.05990541726350784,
-0.00039754441240802407,
0.022808674722909927,
0.028894567862153053,
0.032267145812511444,
-0.03637983649969101,
-0.029698213562369347,
-0.02347785234451294,
-0.06747178733348846,
-0.01943783462047577,
0.07019020617008209,
0.009045292623341084,
0.03375277668237686,
0.001890271552838385,
0.03208664059638977,
0.03472777456045151,
0.01608174666762352,
0.0015879751881584525,
0.02849477156996727,
0.015227098949253559,
0.015456657856702805,
0.000869538402184844,
-0.052019085735082626,
0.010447254404425621,
0.04270542785525322,
0.08131950348615646,
0.012650229968130589,
0.05486360192298889,
0.03885301202535629,
-0.02864832431077957,
-0.0304917823523283,
0.07176674902439117,
0.0516076385974884,
0.023061977699398994,
-0.09213033318519592,
0.019009016454219818,
0.0510329008102417,
0.02822243422269821,
0.030698413029313087,
-0.0625951737165451,
-0.04565035551786423,
-0.019661394879221916,
0.008854793384671211,
0.04970481991767883,
0.021955808624625206,
0.015275523066520691,
0.011094988323748112,
0.024896450340747833,
0.014194468967616558,
-0.040312401950359344,
0.02943326160311699,
-0.022009635344147682,
-0.029727134853601456,
0.017911603674292564,
0.04037296399474144,
-0.01967659406363964,
0.0006754855276085436,
-0.06668759882450104,
-0.031473997980356216,
0.024098338559269905,
-0.07032811641693115,
0.0162642989307642,
-0.026973318308591843,
0.09008323401212692,
-0.03006380796432495,
-0.025773821398615837,
0.02867533266544342,
0.024117616936564445,
0.05050866678357124,
-0.04846189543604851,
0.020759744569659233,
-0.32649219036102295,
0.003397524356842041,
0.05120197683572769,
-0.03243135288357735,
0.07565639913082123,
-0.01862749457359314,
-0.04498421028256416,
-0.051591385155916214,
0.019170066341757774,
0.004037067759782076,
0.06378068774938583,
0.034185778349637985,
-0.03673906996846199,
0.003945067059248686,
-0.05535091832280159,
0.03740530461072922,
0.030359063297510147,
-0.02408505603671074,
-0.0024205935187637806,
0.0013350251829251647,
0.003964597824960947,
-0.000708515930455178,
0.05076923966407776,
0.028242457658052444,
0.03260987251996994,
-0.044215232133865356,
0.10947740077972412,
0.0074119120836257935,
0.04886573925614357,
-0.0435149185359478,
-0.011374128051102161,
0.002174997702240944,
0.003676803084090352,
-0.028034739196300507,
0.03550379350781441,
0.007271820213645697,
-0.05873209238052368,
0.01460269559174776,
-0.005264658946543932,
-0.01948373019695282,
-0.010583262890577316,
0.030796363949775696,
0.012163075618445873,
-0.07458411902189255,
-0.008296875283122063,
0.03074299730360508,
0.010906213894486427,
-0.04602830484509468,
-0.01601036638021469,
0.0032643398735672235,
-0.007982103154063225,
-0.012052466161549091,
0.010946805588901043,
0.08317534625530243,
-0.015006639063358307,
-0.008192168548703194,
-0.017723912373185158,
0.006301506422460079,
-0.04730304330587387,
0.017642274498939514,
0.002322418848052621,
0.020454736426472664,
0.037471525371074677,
-0.009579155594110489,
0.0194572564214468,
0.0009752532350830734,
0.003909021615982056,
-0.025630943477153778,
0.04846027493476868,
0.01841934211552143,
0.04191471263766289,
0.1046406701207161,
-0.0575849786400795,
-0.020578721538186073,
0.05241282656788826,
0.051265839487314224,
0.013696709647774696,
-0.06788701564073563,
-0.04221637174487114,
0.006537558976560831,
0.0345926433801651,
-0.08271447569131851,
0.045995309948921204,
-0.013536582700908184,
0.021708833053708076,
0.018963484093546867,
0.012475921772420406,
-0.025242818519473076,
0.058814097195863724,
0.06155753880739212,
0.010188601911067963,
-0.012563283555209637,
-0.012978234328329563,
0.010193804278969765,
-0.006509071681648493,
0.07480897009372711,
-0.278311163187027,
0.04074883833527565,
-0.03770413622260094,
-0.024140501394867897,
0.004848790820688009,
0.03920300677418709,
0.005964603740721941,
-0.04505974426865578,
0.0025816913694143295,
0.015919899567961693,
0.03130149841308594,
0.0064232973381876945,
0.0812210887670517,
-0.05453158915042877,
-0.030403902754187584,
0.01697731763124466,
0.007383081596344709,
-0.0034959400072693825,
0.013538730330765247,
-0.01004762016236782,
0.06919050216674805,
-0.028877535834908485,
0.1244816780090332,
-0.003490221919491887,
-0.023669971153140068,
0.04336077719926834,
0.001740280189551413,
0.01591331511735916,
0.009196796454489231,
0.005516337230801582,
0.051830001175403595,
0.00907809380441904,
0.09068434685468674,
0.009348162449896336,
0.037084974348545074,
0.018932200968265533,
0.05630484223365784,
-0.04682239145040512,
-0.01783616654574871,
-0.007689900696277618,
-0.06264085322618484,
0.005061356350779533,
-0.08847035467624664,
-0.05462634190917015,
0.02783411741256714,
-0.0912773609161377,
0.02369190938770771,
-0.05414653196930885,
-0.011009374633431435,
0.017806418240070343,
0.002787521341815591,
-0.02558423951268196,
0.006290791090577841,
0.03163304179906845,
0.04309229180216789,
-0.0222273301333189,
0.013614168390631676,
-0.00652712769806385,
-0.028902584686875343,
-0.030853012576699257,
0.02635779045522213,
-0.015250361524522305,
-0.0832139104604721,
0.0711994469165802,
-0.02838090993463993
] |
PMID:27163 | Stabilization of human fibroblast interferon purified on concanavalin A-agarose. | Human fibroblast interferon, obtained by chromatography on concanavalin A-agarose, was stable for at least a month in 30--50 per cent ethylene glycol at 4 degrees, --20 degrees, and --70 degrees C. The succinct point of the present finding is that human fibroblast interferon may be stabilized by ethylene glycol alone without the addition of bovine serum albumin and 'back-contamination' of the interferon preparation. | Stabilization of human fibroblast interferon purified on concanavalin A-agarose. Human fibroblast interferon, obtained by chromatography on concanavalin A-agarose, was stable for at least a month in 30--50 per cent ethylene glycol at 4 degrees, --20 degrees, and --70 degrees C. The succinct point of the present finding is that human fibroblast interferon may be stabilized by ethylene glycol alone without the addition of bovine serum albumin and 'back-contamination' of the interferon preparation. | [
-0.03272799029946327,
-0.015465965494513512,
-0.00865078903734684,
-0.0137095358222723,
-0.01134863868355751,
-0.021086975932121277,
0.010908291675150394,
0.04570438712835312,
-0.08802242577075958,
-0.006844934541732073,
0.02283587120473385,
-0.07689327001571655,
-0.008515797555446625,
0.0008957427344284952,
-0.05463032051920891,
0.008362101390957832,
0.040817659348249435,
0.027085212990641594,
-0.11769072711467743,
0.04994562640786171,
-0.018460463732481003,
-0.09496201574802399,
0.0009064763435162604,
-0.0399763397872448,
0.005370904225856066,
0.020506596192717552,
0.00047579052625223994,
-0.03841054439544678,
-0.018357349559664726,
-0.21484865248203278,
0.003378569148480892,
-0.06284736841917038,
0.00981063861399889,
-0.0342869758605957,
-0.031071176752448082,
-0.01340872049331665,
-0.019835276529192924,
-0.04581849277019501,
-0.0010654389625415206,
0.07551848888397217,
0.0880495235323906,
0.02083565481007099,
-0.04109322652220726,
0.023184742778539658,
0.03973807394504547,
0.013330732472240925,
-0.08486679941415787,
-0.008396752178668976,
0.0954684242606163,
0.04755581542849541,
-0.0039497907273471355,
0.004607636481523514,
-0.020275576040148735,
0.0529368631541729,
-0.06559720635414124,
0.05035717040300369,
0.011607603169977665,
-0.0076898327097296715,
-0.00021145306527614594,
0.036966487765312195,
0.010735655203461647,
0.006556901149451733,
-0.14063741266727448,
0.1267002522945404,
0.029247518628835678,
0.023759042844176292,
0.03588985651731491,
-0.07090815901756287,
0.01230124942958355,
0.02058262936770916,
-0.09333154559135437,
0.06852256506681442,
0.04242691770195961,
0.03981117904186249,
0.010885674506425858,
-0.008069134317338467,
0.012265580706298351,
-0.04876145347952843,
0.06507887691259384,
0.07876405119895935,
0.012422624975442886,
0.02784031443297863,
0.012831563130021095,
0.008905821479856968,
-0.02566736936569214,
-0.01052570529282093,
0.011880957521498203,
-0.026070332154631615,
0.0007444140501320362,
0.08676216006278992,
0.010017956607043743,
0.01737610436975956,
0.004205011297017336,
0.0587114542722702,
-0.08739808201789856,
0.04458111524581909,
0.00558491563424468,
0.029568996280431747,
0.04133263975381851,
0.3476223051548004,
-0.007903513498604298,
-0.0366903580725193,
-0.07667310535907745,
-0.007966924458742142,
0.021455736830830574,
-0.022279147058725357,
0.004620385356247425,
0.014912405051290989,
0.023783516138792038,
0.03362274169921875,
-0.0005268510431051254,
0.03858417645096779,
0.024514218792319298,
-0.014891629107296467,
0.016667073592543602,
0.030024971812963486,
0.04043402522802353,
-0.05456622689962387,
-0.01031368039548397,
0.05855385586619377,
0.038290079683065414,
0.019264595583081245,
0.058658942580223083,
-0.035621289163827896,
0.05842394754290581,
0.05437444895505905,
0.04120651260018349,
0.0631067231297493,
0.029976408928632736,
0.04391912370920181,
0.041869040578603745,
0.02474265918135643,
0.025228146463632584,
-0.015323910862207413,
-0.012205155566334724,
0.03267194330692291,
-0.020937098190188408,
-0.02563001960515976,
0.04158639907836914,
-0.041852790862321854,
0.013873937539756298,
-0.05779155716300011,
-0.029606331139802933,
-0.06111782789230347,
0.020311223343014717,
0.008086915127933025,
0.0033511952497065067,
0.0641021877527237,
-0.0004704694147221744,
-0.022059708833694458,
0.0076522259041666985,
0.07814803719520569,
-0.011073277331888676,
-0.020096084102988243,
0.033936429768800735,
0.02604256197810173,
-0.007016825024038553,
-0.053819626569747925,
-0.011133797466754913,
-0.056030016392469406,
-0.055962495505809784,
0.032359227538108826,
-0.07499881088733673,
0.0782802551984787,
-0.014442145824432373,
-0.05378834903240204,
-0.03966786339879036,
0.014440258964896202,
0.060827068984508514,
0.012929072603583336,
0.06695560365915298,
-0.03340527042746544,
-0.00130464939866215,
-0.009473602287471294,
-0.008528772741556168,
-0.0038167242892086506,
-0.039014529436826706,
0.006845799274742603,
0.04550478979945183,
0.047911208122968674,
-0.0170489102602005,
-0.0038568314630538225,
-0.007969528436660767,
0.04342753067612648,
0.03787847235798836,
-0.04646933078765869,
-0.030503172427415848,
0.02938149869441986,
0.022043069824576378,
0.02983628772199154,
-0.019419753924012184,
0.04747076332569122,
-0.06657301634550095,
0.07974039763212204,
-0.04914893954992294,
0.007974562235176563,
-0.02737240679562092,
-0.007462730165570974,
0.009296340867877007,
-0.025118814781308174,
0.09061639755964279,
-0.052339352667331696,
0.0041504898108541965,
0.040964700281620026,
0.017312834039330482,
0.058080967515707016,
0.0029303126502782106,
0.008778007701039314,
0.04119425266981125,
0.007035398855805397,
-0.05581606552004814,
0.06974903494119644,
-0.04614914581179619,
-0.05659835785627365,
-0.10415812581777573,
0.04027253016829491,
-0.013296819292008877,
-0.052011605352163315,
-0.003518919460475445,
0.014664262533187866,
0.05283002927899361,
0.046278320252895355,
-0.021841447800397873,
-0.30748432874679565,
-0.020025061443448067,
-0.007977153174579144,
-0.02422616444528103,
0.056624241173267365,
-0.08164007216691971,
0.014966277405619621,
-0.017375875264406204,
-0.003932339139282703,
-0.018015287816524506,
0.05576130002737045,
0.08644530177116394,
-0.03547689691185951,
-0.032622095197439194,
-0.010562452487647533,
0.006138224620372057,
0.03612396866083145,
-0.05586552247405052,
0.057880330830812454,
-0.008111643604934216,
-0.038941506296396255,
-0.006346145179122686,
-0.025380967184901237,
0.04078686982393265,
0.04680563136935234,
-0.019611874595284462,
0.12714539468288422,
-0.035405464470386505,
-0.010585892014205456,
-0.009071907959878445,
0.023312972858548164,
0.035950593650341034,
-0.06524241715669632,
-0.04825505614280701,
-0.05152556300163269,
-0.009496733546257019,
-0.05727233365178108,
-0.020308982580900192,
0.0064819990657269955,
0.014985769055783749,
0.029704388231039047,
0.004418603610247374,
0.02453223243355751,
-0.053773749619722366,
-0.01731315441429615,
-0.0009001180878840387,
-0.01571642979979515,
-0.0003902389435097575,
-0.03543199226260185,
0.005607922561466694,
0.05123092979192734,
-0.04551912844181061,
0.014634951949119568,
0.024265164509415627,
-0.03449249640107155,
0.04175461828708649,
-0.018658742308616638,
0.01939946785569191,
-0.03806167468428612,
0.03156408667564392,
-0.011285512708127499,
0.011016136035323143,
-0.001168032526038587,
0.020107077434659004,
-0.019396241754293442,
-0.010415462777018547,
0.054872650653123856,
-0.01994236931204796,
0.06961990892887115,
0.04014682397246361,
-0.018096446990966797,
0.051371827721595764,
-0.035966657102108,
0.0432552807033062,
0.07500142604112625,
0.0447571687400341,
0.0049684555269777775,
-0.004432424437254667,
-0.13471317291259766,
0.027427712455391884,
0.044377949088811874,
-0.041159309446811676,
0.037201009690761566,
-0.024270493537187576,
0.00701876962557435,
0.02182633802294731,
-0.008359690196812153,
0.0038697689305990934,
0.07472766935825348,
0.037677355110645294,
0.04941432178020477,
0.01862027309834957,
-0.012493489310145378,
-0.04739151522517204,
-0.004190166015177965,
-0.016514241695404053,
-0.2459041327238083,
0.03458366543054581,
-0.003300267504528165,
0.018231919035315514,
-0.025455696508288383,
-0.015757931396365166,
0.017154544591903687,
-0.021973399445414543,
-0.050633516162633896,
0.030857834964990616,
-0.021013842895627022,
0.028753522783517838,
0.06885544955730438,
-0.025580113753676414,
0.04688902944326401,
0.011268491856753826,
0.05146880820393562,
-0.08518129587173462,
0.01904228702187538,
-0.06971003860235214,
0.027335669845342636,
-0.08670669049024582,
0.1113152876496315,
0.007652775384485722,
-0.047417350113391876,
0.06902657449245453,
-0.01722036302089691,
0.011582973413169384,
-0.06852441281080246,
-0.0012057520216330886,
0.03773080185055733,
-0.02875674143433571,
-0.015824558213353157,
0.007826314307749271,
0.020661884918808937,
-0.007207097485661507,
-0.035841647535562515,
0.03700302913784981,
0.007539630867540836,
-0.012885686941444874,
-0.05729982256889343,
-0.011552996933460236,
-0.042121753096580505,
-0.08124719560146332,
0.04426194727420807,
-0.09877291321754456,
-0.01304937805980444,
-0.05211358144879341,
0.006065669935196638,
-0.023715265095233917,
-0.021345434710383415,
0.00916560459882021,
0.04150984808802605,
-0.011388891376554966,
-0.03542089834809303,
-0.02396901324391365,
0.014578983187675476,
-0.0029699867591261864,
-0.04637598618865013,
0.036908429116010666,
-0.04905334860086441,
0.02019757404923439,
-0.07234172523021698,
0.07920491695404053,
0.0020972061902284622
] |
PMID:27164 | Inhibition of thermogenic drinking by beta-adrenergic antagonists. | The thirst induced by transfer of rats from air at 5 degrees C to air at 25 degrees C (thermogenic drink) was inhibited by d,1-, but not d-propranolol (6 mg/kg body weight) administered 0.5 h prior to removal from cold. The thermogenic drink was not inhibited by a similar administration of the beta1-adrenergic antagonist, practolol (50 and 150 mg/kg body weight), but was inhibited by the beta2-adrenergic antagonist, butoxamine (35 mg/kg body weight). The results are consistent with the suggestion that beta2-adrenergic receptors play a role in mediation of the thermogenic drink in rats. | Inhibition of thermogenic drinking by beta-adrenergic antagonists. The thirst induced by transfer of rats from air at 5 degrees C to air at 25 degrees C (thermogenic drink) was inhibited by d,1-, but not d-propranolol (6 mg/kg body weight) administered 0.5 h prior to removal from cold. The thermogenic drink was not inhibited by a similar administration of the beta1-adrenergic antagonist, practolol (50 and 150 mg/kg body weight), but was inhibited by the beta2-adrenergic antagonist, butoxamine (35 mg/kg body weight). The results are consistent with the suggestion that beta2-adrenergic receptors play a role in mediation of the thermogenic drink in rats. | [
-0.005932507570832968,
0.022648122161626816,
0.04620308801531792,
0.01737833023071289,
0.00905496347695589,
-0.04196980595588684,
0.07165411114692688,
0.012662461027503014,
-0.07189761102199554,
-0.07129578292369843,
-0.02402513474225998,
-0.0455203503370285,
0.014419712126255035,
0.0504656657576561,
-0.040932487696409225,
0.004790521692484617,
0.07173333317041397,
0.005743896588683128,
-0.0261643398553133,
0.007167678792029619,
-0.001104458817280829,
-0.02647520788013935,
0.027346177026629448,
0.021112604066729546,
-0.00279321544803679,
0.006453521549701691,
-0.009234887547791004,
0.024666408076882362,
-0.047960955649614334,
-0.16151578724384308,
0.008502200245857239,
-0.04504882171750069,
0.05788498371839523,
-0.05163014680147171,
-0.05284397304058075,
0.008058944717049599,
-0.029070770367980003,
0.0222301222383976,
-0.023234540596604347,
0.0342375673353672,
0.019492756575345993,
0.008799395523965359,
-0.03794649615883827,
0.018619541078805923,
0.010050391778349876,
0.03711193427443504,
-0.019508516415953636,
0.00633784057572484,
0.08283576369285583,
0.019161418080329895,
0.02864057570695877,
-0.013863436877727509,
-0.019970837980508804,
0.09226828068494797,
0.026605138555169106,
0.03200455382466316,
-0.015595341101288795,
0.015304319560527802,
0.017332101240754128,
0.03728894144296646,
-0.06125267595052719,
0.034471310675144196,
-0.1648372858762741,
0.0670463964343071,
0.03078525885939598,
0.012173235416412354,
-0.038280364125967026,
0.03688286244869232,
0.021857308223843575,
0.0679694339632988,
0.010358769446611404,
0.0745059996843338,
0.008256959728896618,
-0.007718062028288841,
-0.019425328820943832,
-0.0009249142021872103,
-0.03530414029955864,
0.015977898612618446,
0.021646002307534218,
0.04507676512002945,
0.03499269485473633,
-0.02594577521085739,
0.014509239234030247,
-0.05417381599545479,
-0.013536830432713032,
-0.04866672307252884,
0.09043069183826447,
-0.009014427661895752,
-0.02465965785086155,
-0.024372495710849762,
-0.08787967264652252,
-0.006644856184720993,
0.0007048217230476439,
0.00026700994931161404,
-0.04173818975687027,
0.03976338729262352,
-0.016119342297315598,
-0.03798491507768631,
0.011994360014796257,
0.3304985761642456,
0.039382416754961014,
0.09580640494823456,
-0.046152740716934204,
0.02274247258901596,
0.018063977360725403,
0.011587465181946754,
-0.008393113501369953,
-0.029202718287706375,
-0.00945705734193325,
0.03355599194765091,
0.02350713312625885,
0.032675161957740784,
0.038943126797676086,
-0.02184608392417431,
0.014020929113030434,
-0.00585121801123023,
0.03882678970694542,
0.02495492435991764,
-0.04145073890686035,
0.01710195653140545,
-0.04710856080055237,
-0.01693691872060299,
0.08177657425403595,
0.02903248555958271,
0.0014255413552746177,
-0.03525596112012863,
-0.036466084420681,
0.11767788231372833,
-0.028274210169911385,
-0.011376450769603252,
0.06117671728134155,
-0.007403686176985502,
-0.010788146406412125,
-0.004151712637394667,
0.03852647542953491,
-0.048469800502061844,
0.030674714595079422,
-0.035379841923713684,
0.03607221692800522,
-0.021526111289858818,
-0.021316979080438614,
-0.008356591686606407,
0.0758197009563446,
-0.11908143013715744,
-0.02122923918068409,
0.050264108926057816,
0.031129702925682068,
-0.047992512583732605,
-0.08522140234708786,
0.03981197625398636,
-0.0031471510883420706,
0.0065244995057582855,
0.0007889210246503353,
0.03241627663373947,
-0.028395261615514755,
0.02194938436150551,
0.0203090813010931,
-0.00792208407074213,
-0.03329186886548996,
0.013860751874744892,
-0.022369319573044777,
-0.038328055292367935,
0.019416937604546547,
-0.0014213917311280966,
0.01317682210355997,
0.012086878530681133,
0.0001009901097859256,
-0.02041306160390377,
0.012895180843770504,
0.04839333891868591,
0.02497558481991291,
0.007568745408207178,
-0.061009731143713,
-0.05006995424628258,
-0.0042566400952637196,
0.026535943150520325,
0.06040278077125549,
-0.0050443620420992374,
0.03345445916056633,
-0.021665887907147408,
0.03761747106909752,
-0.026156723499298096,
0.025437498465180397,
-0.03321431949734688,
0.05352991819381714,
0.030962100252509117,
-0.06426964700222015,
-0.0820092186331749,
-0.0007716842228546739,
0.09275767207145691,
-0.0403900109231472,
-0.030705329030752182,
-0.06397439539432526,
-0.014009435661137104,
-0.003914846107363701,
-0.0077121686190366745,
-0.024275118485093117,
0.050305627286434174,
-0.01950339414179325,
0.04530857875943184,
0.05070843547582626,
0.01630432903766632,
-0.012646586634218693,
0.025971129536628723,
0.03869345039129257,
0.07601628452539444,
0.017866656184196472,
-0.043692491948604584,
0.017873581498861313,
-0.030540667474269867,
-0.014600875787436962,
0.047638650983572006,
0.02088477648794651,
-0.019726315513253212,
-0.017893312498927116,
0.0694916769862175,
0.015854893252253532,
-0.02764386124908924,
-0.009987699799239635,
-0.0024256876204162836,
0.03837597742676735,
-0.005539942532777786,
0.02252076379954815,
-0.3307478129863739,
-0.020381374284625053,
-0.01108166016638279,
-0.0395343080163002,
0.014185978099703789,
-0.07688401639461517,
0.09088363498449326,
-0.09268690645694733,
-0.050646256655454636,
-0.01838112249970436,
0.02818734385073185,
0.02481040358543396,
-0.01596064120531082,
-0.02015913650393486,
-0.037969596683979034,
0.038363199681043625,
0.06417127698659897,
-0.04192757233977318,
0.037965524941682816,
0.0174095556139946,
0.015999235212802887,
-0.014416959136724472,
0.0029971515759825706,
0.007455627433955669,
-0.003294782480224967,
0.022907869890332222,
0.08507601916790009,
0.044807206839323044,
-0.022738877683877945,
-0.002488543512299657,
0.04750768467783928,
0.06898745149374008,
0.015424731187522411,
-0.13225091993808746,
-0.00399975897744298,
-0.05713190138339996,
0.0339391715824604,
-0.009763744659721851,
0.021248415112495422,
0.002657592063769698,
-0.021147234365344048,
0.0430404432117939,
-0.024344926699995995,
-0.0393204391002655,
-0.05690611153841019,
-0.03174697607755661,
-0.0007660790579393506,
0.0099859107285738,
-0.05350875109434128,
-0.032492104917764664,
0.013946705497801304,
0.04848276451230049,
0.014070373959839344,
-0.05168398097157478,
0.03203516826033592,
-0.02896042726933956,
-0.09807734936475754,
0.011969768442213535,
-0.00824091024696827,
-0.029025806114077568,
0.03265032917261124,
-0.010489142499864101,
0.04509349167346954,
-0.001149055315181613,
-0.043760254979133606,
0.05164594575762749,
0.002329718554392457,
-0.031047562137246132,
0.061144132167100906,
0.08216387033462524,
0.011740578338503838,
0.11781314760446548,
-0.03097390942275524,
0.018125830218195915,
0.052991852164268494,
-0.002875477774068713,
-0.012349081225693226,
-0.08097463846206665,
-0.018145795911550522,
0.054895851761102676,
-0.011729702353477478,
-0.029719330370426178,
0.022600598633289337,
0.0008283078204840422,
-0.007505892775952816,
-0.023530473932623863,
-0.0028355871327221394,
-0.015964582562446594,
0.06867051124572754,
0.013406157493591309,
-0.03237247094511986,
0.04214181378483772,
-0.06729388236999512,
-0.016662174835801125,
0.09112850576639175,
0.06065865606069565,
-0.2725025713443756,
-0.026266343891620636,
0.04775730147957802,
-0.01559470221400261,
-0.013973494060337543,
-0.007594380062073469,
0.014897827990353107,
-0.05324506014585495,
-0.09827134758234024,
-0.02382928505539894,
-0.04890519753098488,
0.01051386259496212,
0.0802498385310173,
0.07980077713727951,
-0.08838861435651779,
-0.06369120627641678,
0.011508029885590076,
-0.017459305003285408,
0.002095363335683942,
-0.08089899271726608,
0.003415307030081749,
-0.0032016660552471876,
0.13307543098926544,
0.0019257394596934319,
-0.042643774300813675,
0.08305990695953369,
-0.007553002331405878,
-0.000902702973689884,
-0.05278919264674187,
0.031191568821668625,
-0.04626217484474182,
-0.002141674282029271,
0.0655854269862175,
-0.036752283573150635,
0.031238608062267303,
0.047324288636446,
-0.022976627573370934,
0.005959851201623678,
0.04132862016558647,
-0.030605865642428398,
-0.024919185787439346,
0.030078645795583725,
-0.08169496804475784,
-0.0537734292447567,
0.058182332664728165,
-0.05933843553066254,
-0.0375860258936882,
-0.011288447305560112,
-0.03767389431595802,
0.033601559698581696,
-0.002842315938323736,
-0.0684475526213646,
0.020522478967905045,
0.029957063496112823,
-0.006956786382943392,
-0.04396490380167961,
-0.03968050330877304,
-0.03246073052287102,
0.04348321631550789,
-0.010959410108625889,
-0.008985472843050957,
0.022821253165602684,
-0.017338186502456665,
0.0728435218334198,
0.0911390483379364
] |
PMID:27167 | The effects of acridine orange on deoxyribonucleic acid in Escherichia coli. | 1. Acridine Orange inhibits growth of Escherichia coli K12 when incubated at pH 7.9, but not at pH 7.4.2. At a non-permissive temperature for DNA polymerase I, Acridine Orange inhibits growth of a temperature-sensitive strain and also increases the rate of elimination of the F'-Lac plasmid. 3. DNA isolated from cells treated with Acridine Orange under conditions that inhibit growth contains material of low molecular weight, which is absent from DNA isolated from cells treated under conditions in which growth is not impaired. 4. Cells incubated with Acridine Orange at both pH 7.4 and 7.9 suffer degradation of DNA, as shown by loss of labelled DNA from the acid-insoluble fraction, which is not observed with untreated cells at either pH. 5. The results suggest that elimination of the F'-Lac plasmid by Acridine Orange requires inactivation of repair processes. | The effects of acridine orange on deoxyribonucleic acid in Escherichia coli. 1. Acridine Orange inhibits growth of Escherichia coli K12 when incubated at pH 7.9, but not at pH 7.4.2. At a non-permissive temperature for DNA polymerase I, Acridine Orange inhibits growth of a temperature-sensitive strain and also increases the rate of elimination of the F'-Lac plasmid. 3. DNA isolated from cells treated with Acridine Orange under conditions that inhibit growth contains material of low molecular weight, which is absent from DNA isolated from cells treated under conditions in which growth is not impaired. 4. Cells incubated with Acridine Orange at both pH 7.4 and 7.9 suffer degradation of DNA, as shown by loss of labelled DNA from the acid-insoluble fraction, which is not observed with untreated cells at either pH. 5. The results suggest that elimination of the F'-Lac plasmid by Acridine Orange requires inactivation of repair processes. | [
-0.021196868270635605,
0.018167758360505104,
-0.013513757847249508,
0.053077615797519684,
0.08393152058124542,
-0.03626207634806633,
0.030110493302345276,
0.055792901664972305,
-0.03044128604233265,
-0.004291492979973555,
0.06391297280788422,
-0.04175621271133423,
-0.0034293229691684246,
0.011761760339140892,
-0.029909050092101097,
-0.029689643532037735,
-0.06021355837583542,
-0.03562963381409645,
-0.0704970583319664,
-0.025049909949302673,
-0.008565692231059074,
-0.002625323599204421,
-0.03715783730149269,
0.0047152237966656685,
0.01683240383863449,
0.01616896316409111,
-0.020028000697493553,
0.0047466265968978405,
-0.08613725751638412,
-0.2091202586889267,
-0.07231282442808151,
-0.004059135913848877,
0.010898987762629986,
-0.07013135403394699,
-0.024882731959223747,
0.01640426553785801,
-0.043905045837163925,
0.014635427854955196,
-0.03279559314250946,
-0.00038550925091840327,
0.040284764021635056,
0.04207165166735649,
0.00478643923997879,
-0.003935995977371931,
0.008796807378530502,
0.04435252398252487,
0.0019356776028871536,
0.022477760910987854,
0.04257133975625038,
0.003264903323724866,
0.020744064822793007,
-0.022661615163087845,
-0.056322526186704636,
0.06431485712528229,
0.0004604283894877881,
0.019226428121328354,
0.02146226540207863,
-0.0004350955132395029,
0.005629491060972214,
0.038016825914382935,
0.02493351325392723,
0.01575377769768238,
-0.12968195974826813,
0.04779195412993431,
0.04483732581138611,
-0.039842478930950165,
0.0035247700288891792,
-0.032597046345472336,
0.08912476897239685,
0.08257273584604263,
-0.08790697157382965,
-0.01654786616563797,
0.02176719531416893,
0.03669589012861252,
0.04038011282682419,
0.002587821101769805,
-0.0026737279258668423,
-0.03463916480541229,
0.03201325610280037,
-0.026109907776117325,
-0.04074736684560776,
-0.06085989624261856,
0.02787051722407341,
-0.07989208400249481,
-0.015560680069029331,
-0.022421177476644516,
0.016049761325120926,
-0.019206974655389786,
0.003270666114985943,
0.02104923501610756,
0.031089508906006813,
-0.04742898792028427,
0.038989435881376266,
-0.05022492632269859,
-0.026216039434075356,
-0.01709674298763275,
-0.006627105176448822,
-0.05815263092517853,
0.0359821580350399,
0.3368264138698578,
-0.06734070926904678,
0.0052439491264522076,
-0.05656971409916878,
-0.05567871034145355,
-0.005970972590148449,
-0.010558527894318104,
0.01674620248377323,
-0.01083726342767477,
0.03566427528858185,
0.051518846303224564,
0.025456812232732773,
0.04421747848391533,
0.008512682281434536,
-0.016171112656593323,
-0.0007907080580480397,
0.060209911316633224,
0.0310497023165226,
0.01830640807747841,
-0.07577062398195267,
-0.00028301740530878305,
-0.046516451984643936,
-0.05443880707025528,
0.01469636894762516,
0.0033860718831419945,
0.04037943854928017,
0.021036764606833458,
-0.010095304809510708,
0.0929308831691742,
0.019547544419765472,
-0.014188144356012344,
0.03169265389442444,
-0.05628656968474388,
0.07271412014961243,
-0.010084793902933598,
-0.018705934286117554,
0.015780137851834297,
0.04101059213280678,
0.002112510846927762,
0.03744330629706383,
-0.0041407314129173756,
-0.015053042210638523,
-0.04707775264978409,
-0.06627336889505386,
-0.06496789306402206,
-0.04562284052371979,
0.02490433305501938,
0.0023874924518167973,
0.0760553628206253,
-0.019857320934534073,
0.04019962623715401,
-0.03847566246986389,
0.10296443849802017,
0.008232193998992443,
0.06110982224345207,
-0.036465708166360855,
0.03725362569093704,
-0.03899329900741577,
0.001361603382974863,
0.02730969712138176,
0.021520236507058144,
-0.1034175381064415,
0.00508999265730381,
0.0161113403737545,
0.05584830045700073,
-0.023975683376193047,
-0.02203276939690113,
-0.03289265185594559,
0.09419555962085724,
0.026898814365267754,
0.016732821241021156,
0.0032900720834732056,
-0.0435778871178627,
0.030937815085053444,
-0.04664253443479538,
-0.02762456238269806,
-0.0208890363574028,
0.05111922323703766,
0.02217129059135914,
0.038317497819662094,
0.02007591910660267,
0.003618204267695546,
-0.02117151953279972,
0.036246657371520996,
0.02239351160824299,
0.02454136684536934,
-0.03210587427020073,
-0.052710842341184616,
0.03007294237613678,
0.003009204287081957,
0.055269502103328705,
-0.009196567349135876,
-0.022948594763875008,
-0.07041529566049576,
0.07117661833763123,
-0.009101341478526592,
-0.018723072484135628,
0.006699968595057726,
0.057751331478357315,
-0.0316772498190403,
-0.03295096009969711,
0.1106095016002655,
0.026797467842698097,
-0.02645423822104931,
0.015151247382164001,
0.00807928666472435,
0.01707913726568222,
0.05882622301578522,
0.022865887731313705,
-0.018936773762106895,
-0.0347864031791687,
-0.07173950225114822,
0.003099981928244233,
-0.020777953788638115,
-0.00012938065628986806,
-0.055167943239212036,
0.06098009645938873,
0.02699577808380127,
0.008359909057617188,
0.08112389594316483,
-0.009558659046888351,
0.06278935819864273,
-0.06445594877004623,
0.005356979090720415,
-0.32060447335243225,
0.005006060935556889,
-0.017689673230051994,
0.027986275032162666,
0.039955876767635345,
-0.07530400156974792,
0.018279802054166794,
-0.024590080603957176,
0.07401660829782486,
-0.0984194278717041,
0.02406591922044754,
0.03128104656934738,
0.04465029016137123,
-0.040511831641197205,
-0.024178367108106613,
0.04620688408613205,
0.03791603446006775,
-0.029820501804351807,
0.023386508226394653,
0.055351950228214264,
0.020037362352013588,
0.024038702249526978,
0.046986036002635956,
0.006519696209579706,
0.032171256840229034,
0.013630829751491547,
0.08067621290683746,
-0.032278675585985184,
0.007405140437185764,
-0.037789516150951385,
-0.05238708481192589,
0.05745089799165726,
-0.07227563112974167,
-0.06030401587486267,
-0.04044270142912865,
-0.011044645681977272,
-0.0481112115085125,
-0.03500455990433693,
0.02153119444847107,
0.02404598519206047,
0.02481110766530037,
0.021789900958538055,
-0.042544953525066376,
-0.0750870332121849,
-0.02652791328728199,
-0.07650208473205566,
0.0503457635641098,
0.05631951242685318,
-0.020911308005452156,
-0.021850697696208954,
-0.01898181065917015,
0.053475867956876755,
0.03942307457327843,
0.07281170785427094,
0.022693132981657982,
0.008357680402696133,
-0.08343285322189331,
0.06167116388678551,
-0.002588690258562565,
-0.012204539962112904,
0.046326663345098495,
-0.019056271761655807,
0.006605964154005051,
0.021528271958231926,
0.031193885952234268,
0.004790695384144783,
-0.004779020324349403,
-0.020223934203386307,
0.029796995222568512,
0.01653621904551983,
-0.0036156065762043,
0.12560884654521942,
-0.04636291787028313,
0.010120914317667484,
0.010289809666574001,
0.08337846398353577,
-0.04077063873410225,
-0.08728089183568954,
-0.052388887852430344,
0.006575106643140316,
0.009284795261919498,
-0.025498563423752785,
0.039755579084157944,
-0.059822361916303635,
-0.0533689484000206,
0.00023554179642815143,
0.029713964089751244,
-0.022577723488211632,
0.11303826421499252,
0.03712688386440277,
0.05886397138237953,
0.01163493562489748,
-0.020349649712443352,
0.016709402203559875,
0.012431652285158634,
0.0070078447461128235,
-0.24994830787181854,
-0.05294154956936836,
-0.0053354366682469845,
-0.027053922414779663,
-0.012977654114365578,
0.055779825896024704,
0.021934380754828453,
-0.047879546880722046,
-0.005962927360087633,
0.10599362850189209,
-0.023398829624056816,
-0.04172190651297569,
0.03740978240966797,
-0.030756568536162376,
-0.00354540953412652,
0.012790631502866745,
0.071465402841568,
-0.016956735402345657,
0.010898437350988388,
0.013368264771997929,
-0.015272719785571098,
-0.04257511720061302,
0.1164521798491478,
-0.005128868389874697,
0.0024334422778338194,
0.02884829044342041,
-0.032656479626894,
0.013216349296271801,
-0.04599464312195778,
-0.0008493695640936494,
-0.023157823830842972,
0.028725216165184975,
0.0239215437322855,
-0.0471181683242321,
0.08269774913787842,
-0.013697042129933834,
0.009794320911169052,
0.06565655767917633,
-0.013836519792675972,
-0.02724299021065235,
-0.06060608848929405,
0.010030454955995083,
-0.06995012611150742,
-0.04679343104362488,
0.015445533208549023,
-0.022213958203792572,
-0.030934741720557213,
-0.04463552311062813,
-0.022182023152709007,
-0.01658608391880989,
-0.04175354167819023,
-0.03404770791530609,
0.04566655680537224,
0.05096015706658363,
-0.02409159205853939,
-0.02519977279007435,
-0.017310690134763718,
0.029947686940431595,
0.05913582071661949,
-0.04538582265377045,
0.05436102673411369,
-0.014184201136231422,
-0.047359418123960495,
0.10341818630695343,
0.04190655052661896
] |
PMID:27168 | Electron-paramagnetic-resonance studies on nitrate reductase from Escherichia coli K12. | Nitrate reductase was purified from anaerobically grown Escherichia coli K12 by a method based on the Triton X-100 extraction procedure of Clegg[(1976) Biochem. J.153, 533-541], but hydrophobic interaction chromatography was used in the final stage. E.p.r. spectra obtained from the enzyme under a variety of conditions are well resolved and were interpreted with the help of the computer-simulation procedures of Lowe [(1978) Biochem. J.171, 649-651]. Parameters for five molybdenum(V) species from the enzyme are given. The low-pH species (g(av.) 1.9827) is in pH-dependent equilibrium with the high-pH species (g(av.) 1.9762), the pK for interconversion of the species being 8.26. Of a variety of anions tested, only nitrate and nitrite formed complexes with the enzyme (in the low-pH form), giving modified molybdenum(V) e.p.r. spectra. These complexes, as well as the low-pH form of the free enzyme, showed interaction of molybdenum with a single exchangeable proton. The fifth molybdenum(V) species, sometimes detected in small amounts, appears not to be due to functional nitrate reductase. After full reduction of the enzyme with dithionite, addition of nitrate caused reoxidation of molybdenum to the quinquivalent state, in a time less than the enzyme turnover. Activity of the enzyme in the pH range 6-10 is controlled by a pK of 8.2. It is suggested that the low-pH signal-giving species is the form of the enzyme involved in the catalytic cycle. Iron-sulphur and other e.p.r. signals from the enzyme are briefly described and the enzymic reaction mechanism is discussed. | Electron-paramagnetic-resonance studies on nitrate reductase from Escherichia coli K12. Nitrate reductase was purified from anaerobically grown Escherichia coli K12 by a method based on the Triton X-100 extraction procedure of Clegg[(1976) Biochem. J.153, 533-541], but hydrophobic interaction chromatography was used in the final stage. E.p.r. spectra obtained from the enzyme under a variety of conditions are well resolved and were interpreted with the help of the computer-simulation procedures of Lowe [(1978) Biochem. J.171, 649-651]. Parameters for five molybdenum(V) species from the enzyme are given. The low-pH species (g(av.) 1.9827) is in pH-dependent equilibrium with the high-pH species (g(av.) 1.9762), the pK for interconversion of the species being 8.26. Of a variety of anions tested, only nitrate and nitrite formed complexes with the enzyme (in the low-pH form), giving modified molybdenum(V) e.p.r. spectra. These complexes, as well as the low-pH form of the free enzyme, showed interaction of molybdenum with a single exchangeable proton. The fifth molybdenum(V) species, sometimes detected in small amounts, appears not to be due to functional nitrate reductase. After full reduction of the enzyme with dithionite, addition of nitrate caused reoxidation of molybdenum to the quinquivalent state, in a time less than the enzyme turnover. Activity of the enzyme in the pH range 6-10 is controlled by a pK of 8.2. It is suggested that the low-pH signal-giving species is the form of the enzyme involved in the catalytic cycle. Iron-sulphur and other e.p.r. signals from the enzyme are briefly described and the enzymic reaction mechanism is discussed. | [
-0.11487336456775665,
0.03477516397833824,
-0.10356246680021286,
0.06635957956314087,
0.06643795222043991,
-0.0961567610502243,
0.021536212414503098,
0.06302700191736221,
-0.03533192723989487,
-0.013563919812440872,
0.003799736499786377,
-0.09327865391969681,
0.009756551124155521,
-0.02539578266441822,
-0.02101566642522812,
-0.02838796190917492,
-0.022595426067709923,
-0.0008687253575772047,
-0.018476106226444244,
0.002181548858061433,
0.013248222880065441,
-0.007053895387798548,
-0.0013537495397031307,
0.04070296883583069,
0.08018089085817337,
0.028289107605814934,
0.02415572665631771,
-0.0032035894691944122,
-0.08219970762729645,
-0.2240782529115677,
-0.001173275406472385,
-0.01344498060643673,
0.06496592611074448,
-0.015145763754844666,
0.006694464478641748,
0.029575929045677185,
-0.018679367378354073,
-0.009884197264909744,
-0.07222341746091843,
0.0016392137622460723,
0.033460136502981186,
-0.01727243699133396,
0.06621097773313522,
0.04634704440832138,
-0.004605545662343502,
-0.00690868217498064,
0.007994130253791809,
-0.033984508365392685,
0.059680305421352386,
-0.03436132147908211,
0.003804843407124281,
-0.0022249792236834764,
-0.04144677147269249,
0.059902820736169815,
0.031933896243572235,
0.009484575130045414,
0.04030441492795944,
0.0031928936950862408,
-0.0040593077428638935,
0.020167693495750427,
0.06818384677171707,
0.010969545692205429,
-0.1355283111333847,
-0.0038421419449150562,
0.03852684795856476,
0.002304952358826995,
-0.022329149767756462,
-0.04859733209013939,
0.006269970443099737,
0.04285508766770363,
-0.04655160382390022,
0.04118414595723152,
-0.028424711897969246,
-0.03801469877362251,
0.030453715473413467,
-0.0355890654027462,
-0.009620284661650658,
0.012993616051971912,
0.0381014458835125,
-0.047266095876693726,
0.012251368723809719,
0.028723929077386856,
0.016537005081772804,
-0.03274044767022133,
0.014934808015823364,
0.01300274208188057,
0.006295129656791687,
-0.014623389579355717,
-0.008169010281562805,
0.07876039296388626,
-0.008081059902906418,
-0.031848423182964325,
0.024021275341510773,
0.004051664844155312,
-0.049406468868255615,
0.05137024074792862,
0.026370560750365257,
-0.038606319576501846,
-0.008347991853952408,
0.4237027168273926,
-0.011197663843631744,
0.004994980059564114,
-0.012415178120136261,
-0.0010416950099170208,
0.00378247769549489,
-0.07074413448572159,
-0.0009335264912806451,
-0.005627518519759178,
-0.000537452579010278,
0.05327596887946129,
0.0634271427989006,
-0.014074568636715412,
0.017653966322541237,
-0.08994650095701218,
-0.03710309788584709,
0.006174977868795395,
0.025304187089204788,
-0.0314631424844265,
-0.03597770631313324,
-0.01969781704246998,
0.007601501420140266,
0.008670812472701073,
-0.01909191720187664,
-0.004902816377580166,
0.037279464304447174,
-0.04667862877249718,
-0.05481836572289467,
0.05815500766038895,
0.01528218388557434,
0.035917479544878006,
-0.021622465923428535,
0.04298065975308418,
-0.005350494757294655,
-0.043712370097637177,
-0.0337606742978096,
-0.0019527707481756806,
0.061876244843006134,
0.009021751582622528,
0.003872355679050088,
-0.021762767806649208,
-0.04168151319026947,
-0.004790449980646372,
-0.08365592360496521,
-0.047548048198223114,
-0.056725043803453445,
-0.00932922214269638,
0.0032954011112451553,
0.02996584214270115,
-0.0741986483335495,
0.0014963042922317982,
-0.005226151552051306,
0.09426019340753555,
-0.012274157255887985,
0.022668777033686638,
0.028518550097942352,
0.04452115669846535,
-0.006166231818497181,
-0.050810858607292175,
0.017723966389894485,
0.013568157330155373,
-0.08847057819366455,
-0.03174714371562004,
0.03248085454106331,
0.0678635910153389,
0.015626724809408188,
0.007902853190898895,
-0.0308225080370903,
-0.009385534562170506,
0.061922136694192886,
0.0145317567512393,
0.047567542642354965,
0.045846667140722275,
0.02927223965525627,
-0.0475725382566452,
0.004566668067127466,
0.027614304795861244,
0.009243414737284184,
0.025510095059871674,
0.02066967450082302,
-0.0005085559678263962,
0.0017151501961052418,
0.013605996035039425,
0.037088558077812195,
0.072348952293396,
0.04194476082921028,
0.027299964800477028,
0.005150315817445517,
-0.01581655628979206,
-0.04497819393873215,
0.04603558033704758,
-0.006992136128246784,
-0.016765709966421127,
-0.05895348638296127,
0.04454467073082924,
-0.03737080097198486,
-0.00926471408456564,
-0.02913387306034565,
0.01918902061879635,
0.03491204231977463,
-0.01547607034444809,
0.11174844205379486,
0.021438604220747948,
0.015450972132384777,
0.02284892648458481,
-0.014495566487312317,
-0.010105688124895096,
0.04925169423222542,
0.07849807292222977,
0.019792381674051285,
0.04204155504703522,
-0.05488564819097519,
0.00995810329914093,
-0.0962178185582161,
-0.01487462967634201,
-0.07069417089223862,
-0.02022106572985649,
0.058470532298088074,
0.009496169164776802,
0.07140500843524933,
0.028969012200832367,
0.05953264608979225,
-0.07622240483760834,
-0.05478958785533905,
-0.30013057589530945,
-0.015405707061290741,
0.03339040279388428,
0.041017141193151474,
0.05564047396183014,
-0.09526236355304718,
-0.005774227436631918,
-0.010364902205765247,
-0.005301345139741898,
-0.034557096660137177,
0.10245590656995773,
0.04261805862188339,
-0.057067010551691055,
-0.032912880182266235,
0.03758374601602554,
0.023422498255968094,
0.03557738661766052,
-0.09229906648397446,
-0.011972898617386818,
0.08457078784704208,
0.02919205278158188,
-0.0009129608515650034,
0.01779790408909321,
-0.002130143577232957,
0.0425666868686676,
-0.01418666634708643,
0.03281976655125618,
-0.010313950479030609,
0.01689792610704899,
-0.02010231837630272,
0.006294761784374714,
0.03142276778817177,
-0.02294844016432762,
-0.029680121690034866,
-0.059796977788209915,
0.01903395541012287,
0.005040459800511599,
-0.05494164675474167,
0.01007368415594101,
-0.01753224991261959,
-0.006356626283377409,
-0.018855437636375427,
-0.04007819667458534,
-0.057478681206703186,
0.026429755613207817,
-0.01875932328402996,
0.00019022078777197748,
-0.01574747823178768,
0.03152995929121971,
0.0050465925596654415,
0.009539302438497543,
0.02909752167761326,
0.042866840958595276,
0.016559617593884468,
-0.016522327437996864,
-0.023078812286257744,
0.015309777110815048,
0.03696979954838753,
-0.036718450486660004,
-0.08509311079978943,
-0.02462003193795681,
-0.055833857506513596,
-0.04003911837935448,
0.02700227126479149,
0.007955968379974365,
-0.008004499599337578,
0.00952855497598648,
-0.014124403707683086,
0.039116259664297104,
-0.04948926717042923,
-0.00930067989975214,
0.09349016100168228,
-0.009127234108746052,
0.02036947011947632,
0.028250856325030327,
0.054792482405900955,
-0.014861324802041054,
-0.017339134588837624,
-0.0646379292011261,
-0.021420873701572418,
0.01184656098484993,
-0.02433554083108902,
0.07512214034795761,
-0.006634047254920006,
0.001600477728061378,
0.045524757355451584,
0.027127712965011597,
-0.015350248664617538,
0.029833855107426643,
0.036301158368587494,
-0.017293833196163177,
-0.0002648402296472341,
-0.0010077145416289568,
-0.05680375546216965,
0.07559991627931595,
0.008701657876372337,
-0.2433910071849823,
-0.04667054861783981,
0.004499079193919897,
0.01976221613585949,
-0.04203702136874199,
0.06759867072105408,
0.04834141209721565,
-0.03991318866610527,
0.0014406612608581781,
0.07388218492269516,
0.014647000469267368,
0.003032572567462921,
-0.002507055178284645,
0.03884928673505783,
0.04730288311839104,
0.051154445856809616,
0.015464890748262405,
-0.06629657745361328,
0.001900830422528088,
0.022212425246834755,
0.0292259082198143,
-0.015506820753216743,
0.12375873327255249,
-0.02097211591899395,
-0.01487282756716013,
0.06624048203229904,
0.002788991667330265,
0.036357391625642776,
-0.007096122018992901,
-0.016178790479898453,
-0.00393119640648365,
-0.018984604626893997,
0.051531802862882614,
-0.044839147478342056,
-0.004472346510738134,
0.06586013734340668,
-0.0157773494720459,
-0.07120665162801743,
-0.027524059638381004,
-0.01686965860426426,
-0.016615595668554306,
0.014733418822288513,
-0.04971398413181305,
-0.07732011377811432,
0.0717119500041008,
-0.04908670485019684,
-0.060456059873104095,
-0.056854359805583954,
0.023283567279577255,
-0.0011758888140320778,
-0.07909312844276428,
0.003674627747386694,
0.037183016538619995,
0.052977632731199265,
-0.01455357950180769,
0.025884350761771202,
-0.0052957674488425255,
-0.029146838933229446,
0.026613082736730576,
-0.026708533987402916,
0.05967798829078674,
-0.012610022909939289,
-0.018373990431427956,
0.008255183696746826,
0.0043298229575157166
] |
PMID:27169 | Oxygen-binding characteristics of three extracellular haemoglobins of Artemia salina. | The oxygen-binding characteristics of the three extracellular haemoglobins of brine shrimp (Artemia salina) were studied in vitro by using highly purified preparations. Haemoglobin I is induced last in the development of brine shrimps when functional gills are formed. It has the lowest oxygen affinity (p(50) 5.34mmHg), an intermediate Bohr effect (ø -0.09 at 20 degrees C) above pH8 and a temperature-sensitivity (DeltaH -44.8 to -45.6kJ/mol at pH8-9) comparable with those observed with other invertebrate haemoglobins [Weber & Heidemann (1977) Comp. Biochem. Physiol. A57, 151-155]. Haemoglobin II, which is the first to be induced, soon after hatching of nauplius larvae, persists generally throughout the whole adult life. It has an intermediate oxygen affinity (p(50) 3.7mmHg), the highest Bohr effect (ø -0.21 at 20 degrees C) above pH8 and a similar temperature-sensitivity (DeltaH -46.0 to -54.8kJ/mol at pH8-9) as haemoglobin I. However, haemoglobin III, which is induced second several hours after the induction of haemoglobin II but disappearing from the haemolymph in the middle of adult life, has the highest oxygen affinity (p(50) 1.8mmHg), the lowest Bohr effect (ø -0.03 at 20 degrees C) above pH8.5 and a high resistance against temperature variation between 10 and 25 degrees C at pH8.5-9 (DeltaH -22.6 to -23.0kJ/mol). At pH7.5-8, haemoglobin III exhibits a similar temperature-sensitivity under 30 degrees C as do other haemoglobins. All three haemoglobins have a rather low co-operativity, with Hill coefficients (h 1.6-1.9 at pH8.5), which are dependent on both pH and temperature. The highest co-operativity was observed at 20 degrees C and pH9 for haemoglobins I and II, whereas it was at 27 degrees C and pH8.5 for haemoglobin III. Thus the oxygen-binding behaviour of haemoglobin III in vitro is significantly different from those of haemoglobins I and II and indicates possibly its specific physiological role in vivo in the adaptive process in the natural environment. | Oxygen-binding characteristics of three extracellular haemoglobins of Artemia salina. The oxygen-binding characteristics of the three extracellular haemoglobins of brine shrimp (Artemia salina) were studied in vitro by using highly purified preparations. Haemoglobin I is induced last in the development of brine shrimps when functional gills are formed. It has the lowest oxygen affinity (p(50) 5.34mmHg), an intermediate Bohr effect (ø -0.09 at 20 degrees C) above pH8 and a temperature-sensitivity (DeltaH -44.8 to -45.6kJ/mol at pH8-9) comparable with those observed with other invertebrate haemoglobins [Weber & Heidemann (1977) Comp. Biochem. Physiol. A57, 151-155]. Haemoglobin II, which is the first to be induced, soon after hatching of nauplius larvae, persists generally throughout the whole adult life. It has an intermediate oxygen affinity (p(50) 3.7mmHg), the highest Bohr effect (ø -0.21 at 20 degrees C) above pH8 and a similar temperature-sensitivity (DeltaH -46.0 to -54.8kJ/mol at pH8-9) as haemoglobin I. However, haemoglobin III, which is induced second several hours after the induction of haemoglobin II but disappearing from the haemolymph in the middle of adult life, has the highest oxygen affinity (p(50) 1.8mmHg), the lowest Bohr effect (ø -0.03 at 20 degrees C) above pH8.5 and a high resistance against temperature variation between 10 and 25 degrees C at pH8.5-9 (DeltaH -22.6 to -23.0kJ/mol). At pH7.5-8, haemoglobin III exhibits a similar temperature-sensitivity under 30 degrees C as do other haemoglobins. All three haemoglobins have a rather low co-operativity, with Hill coefficients (h 1.6-1.9 at pH8.5), which are dependent on both pH and temperature. The highest co-operativity was observed at 20 degrees C and pH9 for haemoglobins I and II, whereas it was at 27 degrees C and pH8.5 for haemoglobin III. Thus the oxygen-binding behaviour of haemoglobin III in vitro is significantly different from those of haemoglobins I and II and indicates possibly its specific physiological role in vivo in the adaptive process in the natural environment. | [
-0.07383759319782257,
0.062273502349853516,
-0.02902972511947155,
0.011575091630220413,
0.06275913864374161,
-0.026943666860461235,
0.11019904166460037,
0.04947652667760849,
-0.03393889591097832,
-0.0019836602732539177,
0.041586145758628845,
-0.08017773926258087,
-0.01972338929772377,
0.014926725067198277,
-0.050765641033649445,
-0.010138032026588917,
0.003990636207163334,
-0.013478485867381096,
-0.0716119185090065,
0.023103099316358566,
0.017596932128071785,
-0.029232587665319443,
0.03801441565155983,
-0.02441452443599701,
0.026142889633774757,
0.06011752784252167,
-0.020339393988251686,
0.016273008659482002,
-0.02690780535340309,
-0.1845141053199768,
-0.03915652632713318,
-0.020140420645475388,
0.010802113451063633,
-0.041256457567214966,
-0.03449585288763046,
0.03707569092512131,
-0.021851520985364914,
-0.04323996976017952,
-0.08572772145271301,
0.008458483032882214,
0.031750574707984924,
0.04835129156708717,
0.010552261024713516,
0.0528552420437336,
-0.05242253094911575,
0.007951822131872177,
0.0017161709256470203,
0.03410005196928978,
0.0676468014717102,
-0.008333404548466206,
-0.05381197854876518,
-0.019505752250552177,
-0.009306542575359344,
0.02852182649075985,
0.03414732962846756,
0.016481900587677956,
-0.000942312995903194,
0.04453880712389946,
-0.0012762232217937708,
0.00509951775893569,
0.03449166566133499,
0.047531116753816605,
-0.07565286010503769,
0.09079811722040176,
0.062428221106529236,
0.006230743136256933,
-0.010715584270656109,
-0.04042528569698334,
0.05667571723461151,
-0.01056851726025343,
-0.04208970069885254,
0.017311081290245056,
0.00642112223431468,
-0.015878504142165184,
0.04089000076055527,
0.02065068669617176,
-0.0431855171918869,
0.0011706665391102433,
0.0599595308303833,
-0.004545701667666435,
-0.056141242384910583,
-0.02667238377034664,
0.0037831803783774376,
0.007896102964878082,
0.031548403203487396,
-0.015548682771623135,
0.042305681854486465,
-0.0625019371509552,
-0.055631428956985474,
-0.013988505117595196,
-0.034020181745290756,
-0.0795404464006424,
-0.04488067328929901,
0.0018533088732510805,
-0.04898219555616379,
0.05232542008161545,
0.005276147276163101,
-0.05394405126571655,
-0.0009213538723997772,
0.38662445545196533,
-0.015938540920615196,
0.024517223238945007,
-0.0354580320417881,
-0.017163198441267014,
0.0856437161564827,
-0.020391305908560753,
-0.011916827410459518,
-0.021119102835655212,
-0.013501429930329323,
0.042756449431180954,
0.005974089726805687,
-0.05130746215581894,
-0.014453223906457424,
-0.01697106473147869,
-0.06277406960725784,
0.04168169945478439,
0.023768115788698196,
-0.021918922662734985,
-0.036699820309877396,
0.0235915407538414,
-0.04172492027282715,
-0.019377635791897774,
0.0713261142373085,
-0.024233220145106316,
0.06574533134698868,
0.01712588220834732,
0.01708713360130787,
0.09733238816261292,
0.06933590024709702,
-0.011944378726184368,
0.0849941074848175,
0.014072990044951439,
-0.027663039043545723,
-0.009726331569254398,
-0.04622885212302208,
-0.04869415983557701,
0.04265456274151802,
-0.052979566156864166,
-0.013296831399202347,
0.003760306164622307,
-0.017245491966605186,
-0.05156036093831062,
0.007721786852926016,
-0.07513343542814255,
-0.11002131551504135,
0.05986504629254341,
0.036118339747190475,
-0.023382587358355522,
-0.0216513779014349,
-0.02558515965938568,
-0.06929634511470795,
0.03560179844498634,
0.0009103271295316517,
0.035749610513448715,
-0.0067469896748661995,
0.0518295019865036,
-0.04219309240579605,
0.06212951987981796,
0.030310839414596558,
-0.04517444595694542,
-0.08260238915681839,
-0.01647479273378849,
-0.01787203922867775,
0.00609939219430089,
0.01635231450200081,
-0.002001193119212985,
-0.009101524949073792,
0.029276574030518532,
0.036998771131038666,
0.024074390530586243,
0.01153725478798151,
-0.02813516929745674,
0.0019174517365172505,
-0.018492640927433968,
0.026159998029470444,
0.018493620678782463,
0.04608877748250961,
0.05719127878546715,
0.00949989352375269,
0.03216763585805893,
0.007610636297613382,
0.03691205009818077,
-0.042280543595552444,
0.04216597229242325,
0.012903210707008839,
0.004413343966007233,
-0.08094503730535507,
0.02001226879656315,
-0.038060009479522705,
0.06780081242322922,
-0.03735528141260147,
0.008979027159512043,
-0.0248103067278862,
0.09880886226892471,
-0.006144343409687281,
-0.010834324173629284,
-0.02371797151863575,
0.021414224058389664,
-0.012930738739669323,
-0.042162392288446426,
0.12503713369369507,
-0.010724950581789017,
0.0013497892068699002,
0.06739504635334015,
0.05355297029018402,
0.020564187318086624,
0.04725915938615799,
0.003696269588544965,
0.047761086374521255,
-0.024042511358857155,
-0.05253051593899727,
0.00699163181707263,
-0.05603034049272537,
-0.027559584006667137,
-0.03766250237822533,
0.027843080461025238,
0.015905052423477173,
0.01104722823947668,
0.03386567160487175,
0.03252503275871277,
0.03107556514441967,
-0.12129086256027222,
-0.003330443287268281,
-0.3345872163772583,
0.006373187992721796,
-0.037742968648672104,
-0.03736894577741623,
0.034658681601285934,
-0.00288434699177742,
0.05006932467222214,
0.025771914049983025,
0.01420033909380436,
-0.053332336246967316,
-0.0060994974337518215,
0.01990526355803013,
0.0009478048887103796,
-0.06903138011693954,
0.02848205156624317,
0.04314248263835907,
0.09510596096515656,
-0.03940476104617119,
0.02661209926009178,
0.05819108337163925,
0.02391001209616661,
-0.007548036519438028,
-0.023221297189593315,
0.06726498156785965,
0.08649611473083496,
-0.012570081278681755,
0.09958849847316742,
-0.02929583378136158,
0.009232189506292343,
0.005149999633431435,
0.026249907910823822,
0.014199438504874706,
0.004748419858515263,
-0.05002177879214287,
-0.00869715679436922,
-0.03479951247572899,
-0.042236484587192535,
-0.032630883157253265,
0.05144573375582695,
-0.001353499130345881,
-0.011716716922819614,
0.05597080662846565,
-0.03183172643184662,
-0.029521869495511055,
-0.015046736225485802,
-0.03786665201187134,
0.011589664034545422,
-0.023920664563775063,
-0.008586500771343708,
-0.06634803116321564,
0.039191942662000656,
0.00027004798175767064,
-0.02304467372596264,
-0.0330316424369812,
-0.008827999234199524,
0.0032212925143539906,
-0.026097280904650688,
0.05186323821544647,
-0.0692039281129837,
-0.014480060897767544,
0.027679014950990677,
-0.022168230265378952,
0.011333566159009933,
0.0037030992098152637,
-0.024693751707673073,
0.011499616317451,
0.05353040248155594,
-0.03054223209619522,
0.05764229968190193,
-0.019353678449988365,
0.017086466774344444,
0.07163380831480026,
-0.009732252918183804,
0.023840347304940224,
0.043363023549318314,
-0.006663288455456495,
-0.03098556213080883,
-0.00650477921590209,
-0.0012418347178027034,
-0.005696004256606102,
0.015171433798968792,
-0.026127461344003677,
0.02511381171643734,
-0.015541132539510727,
-0.0023991926573216915,
0.053697794675827026,
0.0009100320166908205,
-0.013865228742361069,
0.04175914078950882,
-0.003945001866668463,
0.0002823298273142427,
-0.04694700613617897,
-0.022360023111104965,
-0.05598287656903267,
0.025974301621317863,
0.05326312407851219,
-0.2739880084991455,
-0.0034394990652799606,
0.0020725794602185488,
0.04332025721669197,
-0.013634132221341133,
0.009916430339217186,
-0.02800188772380352,
-0.11072113364934921,
-0.0003913872060365975,
0.06575420498847961,
0.012429828755557537,
0.029516246169805527,
0.0445893295109272,
0.04861275479197502,
0.020523682236671448,
0.03543327376246452,
-0.01568022184073925,
-0.058482035994529724,
-0.015049976296722889,
-0.04025023803114891,
-0.012842287309467793,
-0.029033225029706955,
0.1394948810338974,
-0.008291056379675865,
-0.018673300743103027,
0.014758473262190819,
-0.001213345560245216,
0.011805586516857147,
-0.11768204718828201,
-0.002513313665986061,
0.03038487210869789,
0.03398144245147705,
0.06785150617361069,
-0.022405430674552917,
0.02660433202981949,
0.07628875225782394,
-0.052747391164302826,
-0.04290233179926872,
0.01456865482032299,
0.009367703460156918,
-0.0032632702495902777,
-0.004725588019937277,
-0.0415344275534153,
0.015444100834429264,
0.06512995809316635,
0.02307392843067646,
-0.055298879742622375,
-0.005828334949910641,
-0.006567640695720911,
0.019352080300450325,
-0.05664806813001633,
-0.03218027576804161,
0.02182980440557003,
0.014890162274241447,
0.007469194009900093,
-0.0266791433095932,
-0.007382896263152361,
-0.014198346063494682,
-0.04308190941810608,
0.0048284586519002914,
0.03929026797413826,
0.03839334100484848,
-0.06838632375001907,
0.044816285371780396,
0.05374564975500107
] |
PMID:27170 | Equilibrium binding of coenzymes and substrates to nicotinamide-adenine dinucleotide phosphate-linked isocitrate dehydrogenase from bovine heart mitochondria. | 1. The stoicheiometries and affinities of ligand binding to isocitrate dehydrogenase were studied at pH 7.0, mainly by measuring changes in NADPH and protein fluorescence. 2. The affinity of the enzyme for NADPH is about 100-fold greater than it is for NADP+ in various buffer/salt solutions, and the affinities for both coenzymes are decreased by Mg2+, phosphate and increase in ionic strength. 3. The maximum binding capacity of the dimeric enzyme for NADPH, from coenzyme fluorescence and protein-fluorescence measurements, and also for NADP+, by ultrafiltration, is 2 mol/mol of enzyme. Protein-fluorescence titrations of the enzyme with NADP+ are apparently inconsistent with this conclusion, indicating that the increase in protein fluorescence caused by NADP+ binding is not proportional to fractional saturation of the binding sites. 4. Changes in protein fluorescence caused by changes in ionic strength and by the binding of substrates, Mg2+ or NADP+ (but not NADPH) are relatively slow, suggesting conformation changes. 5. In the presence of Mg2+, the enzyme binds isocitrate very strongly, and 2-oxoglutarate rather weakly. 6. Evidence is presented for the formation of an abortive complex of enzyme-Mg2+-isocitrate-NADPH in which isocitrate and NADPH are bound much more weakly than in their complexes with enzyme and Mg2+ alone. 7. The results are discussed in relation to the interpretation of the kinetic properties of the enzyme and its behaviour in the mitochondrion. | Equilibrium binding of coenzymes and substrates to nicotinamide-adenine dinucleotide phosphate-linked isocitrate dehydrogenase from bovine heart mitochondria. 1. The stoicheiometries and affinities of ligand binding to isocitrate dehydrogenase were studied at pH 7.0, mainly by measuring changes in NADPH and protein fluorescence. 2. The affinity of the enzyme for NADPH is about 100-fold greater than it is for NADP+ in various buffer/salt solutions, and the affinities for both coenzymes are decreased by Mg2+, phosphate and increase in ionic strength. 3. The maximum binding capacity of the dimeric enzyme for NADPH, from coenzyme fluorescence and protein-fluorescence measurements, and also for NADP+, by ultrafiltration, is 2 mol/mol of enzyme. Protein-fluorescence titrations of the enzyme with NADP+ are apparently inconsistent with this conclusion, indicating that the increase in protein fluorescence caused by NADP+ binding is not proportional to fractional saturation of the binding sites. 4. Changes in protein fluorescence caused by changes in ionic strength and by the binding of substrates, Mg2+ or NADP+ (but not NADPH) are relatively slow, suggesting conformation changes. 5. In the presence of Mg2+, the enzyme binds isocitrate very strongly, and 2-oxoglutarate rather weakly. 6. Evidence is presented for the formation of an abortive complex of enzyme-Mg2+-isocitrate-NADPH in which isocitrate and NADPH are bound much more weakly than in their complexes with enzyme and Mg2+ alone. 7. The results are discussed in relation to the interpretation of the kinetic properties of the enzyme and its behaviour in the mitochondrion. | [
-0.0911787673830986,
0.004126132000237703,
-0.06343162059783936,
0.027808278799057007,
0.043503597378730774,
-0.004997483920305967,
0.0746794044971466,
0.02094980888068676,
-0.018479419872164726,
-0.031000448390841484,
0.04881997033953667,
-0.07439026981592178,
-0.006784580182284117,
0.03225135803222656,
-0.009515064768493176,
-0.010881509631872177,
0.015077225863933563,
-0.012522946111857891,
-0.04816751182079315,
-0.06181083619594574,
-0.021312439814209938,
0.02044779807329178,
0.0038486982230097055,
-0.033406853675842285,
0.04776705428957939,
0.075969398021698,
0.00452439533546567,
0.0012250447180122137,
-0.07116938382387161,
-0.2234019637107849,
0.007042745593935251,
0.014134346507489681,
0.003748448332771659,
-0.024481801316142082,
-0.021205667406320572,
0.008976309560239315,
0.009033932350575924,
-0.013265598565340042,
-0.01965632289648056,
-0.006339013576507568,
0.0575450137257576,
0.0313815176486969,
0.009237063117325306,
-0.001510379952378571,
-0.02302786521613598,
-0.031316276639699936,
-0.055051472038030624,
0.008739763870835304,
0.06281660497188568,
0.002050024224445224,
-0.04480023682117462,
0.006561659276485443,
-0.015550188720226288,
-0.014280775561928749,
0.029606042429804802,
0.03035075217485428,
0.05292751267552376,
0.08907341957092285,
-0.01453457958996296,
-0.01884487457573414,
0.018572458997368813,
0.013820131309330463,
-0.13052834570407867,
0.03533688560128212,
0.09086765348911285,
-0.014989199116826057,
-0.0240787286311388,
-0.04042821377515793,
0.04500043764710426,
0.00906637217849493,
-0.06100013479590416,
0.044111113995313644,
0.0005650692619383335,
-0.07356421649456024,
0.035707924515008926,
0.0037794229574501514,
0.01647118851542473,
0.0008623889298178256,
0.035018131136894226,
-0.013201053254306316,
-0.04275619983673096,
-0.016527801752090454,
-0.034252531826496124,
-0.03278849273920059,
-0.007600994314998388,
0.03646990656852722,
0.04331457242369652,
-0.053786877542734146,
-0.009212658740580082,
0.015318240039050579,
0.01864483393728733,
-0.08007961511611938,
0.01444630604237318,
-0.01568380557000637,
-0.09673434495925903,
0.09579857438802719,
0.023270847275853157,
0.028384068980813026,
0.004442926961928606,
0.3531993329524994,
-0.032558295875787735,
0.07702001929283142,
-0.060416702181100845,
-0.05041740462183952,
-0.0193277969956398,
-0.06714468449354172,
-0.03124154359102249,
0.035396233201026917,
0.001182231237180531,
0.04417039453983307,
0.048321180045604706,
-0.0022676477674394846,
-0.03143366798758507,
-0.060025475919246674,
-0.04302315413951874,
-0.016679130494594574,
-0.036779388785362244,
-0.006862358655780554,
-0.039237502962350845,
0.005382748320698738,
0.024291565641760826,
0.02948865108191967,
0.0187849048525095,
0.016569295898079872,
0.05000445619225502,
0.009355837479233742,
-0.024827541783452034,
0.0960521325469017,
0.1078011691570282,
0.02221342734992504,
0.044846512377262115,
0.04716099798679352,
-0.04462510347366333,
-0.007590043358504772,
-0.0028766992036253214,
-0.007255156058818102,
0.005709791090339422,
-0.058511555194854736,
0.072658009827137,
-0.0016609716694802046,
-0.023045804351568222,
-0.06155351921916008,
-0.04588596522808075,
-0.03706846386194229,
-0.05880442634224892,
0.08758334815502167,
-0.00037549802800640464,
0.05023766681551933,
-0.0032315899152308702,
-0.011806401424109936,
0.02274114266037941,
0.051147155463695526,
-0.031857941299676895,
0.04836254194378853,
-0.04495524615049362,
0.01610955223441124,
-0.0009248050046153367,
-0.01406982820481062,
0.02136879973113537,
-0.029313653707504272,
-0.1564834862947464,
-0.040126241743564606,
0.013017106801271439,
0.036944933235645294,
0.01889180950820446,
-0.019672872498631477,
-0.03409884124994278,
-0.01756514236330986,
-0.025932133197784424,
-0.030254174023866653,
0.010177345015108585,
-0.01578332483768463,
0.02900666557252407,
-0.01962566003203392,
-0.013176143169403076,
0.04287181794643402,
-0.005581294652074575,
0.03740287944674492,
0.002405770355835557,
-0.03952517360448837,
-0.013710295781493187,
-0.007614701986312866,
-0.014050744473934174,
0.06315650790929794,
0.04576893150806427,
-0.0010465426603332162,
-0.05377748981118202,
0.053556665778160095,
-0.013726286590099335,
0.05850585922598839,
-0.005075407214462757,
0.0064183869399130344,
-0.07409624010324478,
0.04613532871007919,
-0.08195355534553528,
-0.024639135226607323,
-0.012486864812672138,
0.016266200691461563,
-0.012473399750888348,
-0.00909994263201952,
0.13888874650001526,
0.019863102585077286,
-0.00952695682644844,
0.05742852762341499,
-0.007090348284691572,
0.002662390936166048,
-0.03324896842241287,
0.07258238643407822,
0.04194212704896927,
0.06750219315290451,
-0.04693175107240677,
-0.009628050960600376,
-0.0797785222530365,
0.008016284555196762,
-0.06861357390880585,
0.011162113398313522,
-0.013188655488193035,
0.0435105562210083,
0.04805250093340874,
0.03736002743244171,
0.03577064350247383,
-0.06227576732635498,
-0.04228339344263077,
-0.31062862277030945,
-0.018766844645142555,
0.004763823002576828,
-0.01621546596288681,
0.06957492232322693,
0.009989586658775806,
0.0662006139755249,
-0.016381731256842613,
-0.032008010894060135,
-0.038196761161088943,
-0.011880035512149334,
0.02905857376754284,
-0.02075175568461418,
-0.0673295184969902,
-0.0432625412940979,
-0.006208987440913916,
-0.004120059311389923,
-0.0731443390250206,
-0.020823638886213303,
-0.010684303008019924,
0.03626258671283722,
-0.015364879742264748,
-0.02604612149298191,
-0.005209887865930796,
0.032884303480386734,
-0.0402604341506958,
0.09088318794965744,
-0.02483540028333664,
-0.01543671078979969,
0.014318278059363365,
-0.015222115442156792,
0.02912299335002899,
-0.09352009743452072,
-0.06975868344306946,
-0.05539337545633316,
-0.014062393456697464,
-0.03901113569736481,
-0.07138289511203766,
0.05236300826072693,
0.0012705224798992276,
0.014291434548795223,
0.06790448725223541,
0.00006710188608849421,
-0.03518863767385483,
-0.01756717637181282,
-0.005521700717508793,
0.05382680892944336,
-0.007304925937205553,
-0.002656680764630437,
0.00322988023981452,
0.061465002596378326,
-0.0029738927260041237,
-0.00015404426085297018,
-0.062300555408000946,
0.014556857757270336,
0.0017430993029847741,
-0.014236540533602238,
-0.014665820635855198,
-0.046329014003276825,
0.021194539964199066,
-0.004663756582885981,
0.024121534079313278,
0.0009921644814312458,
0.034801047295331955,
0.0109859062358737,
-0.0037264088168740273,
0.01480893138796091,
-0.023664535954594612,
0.021906128153204918,
-0.003185579553246498,
0.04936470836400986,
0.0713592916727066,
-0.012488416396081448,
0.02579348348081112,
0.04226392135024071,
0.03898300230503082,
-0.03778127580881119,
-0.004122740589082241,
0.0007570629823021591,
0.019145697355270386,
0.0036066018510609865,
-0.019253818318247795,
0.10058799386024475,
-0.007794717792421579,
0.01594087854027748,
0.020350875332951546,
0.04905034974217415,
-0.053511157631874084,
-0.01001762505620718,
0.011787885800004005,
0.007213884964585304,
0.0007502792286686599,
0.0007749825017526746,
-0.023474637418985367,
-0.001058298395946622,
0.07195309549570084,
-0.25579026341438293,
0.008417983539402485,
-0.018058661371469498,
0.03083937056362629,
-0.026005052030086517,
0.06617122143507004,
0.07662006467580795,
-0.023376472294330597,
-0.04290831461548805,
0.05000936612486839,
0.02018287591636181,
0.02851877547800541,
0.03812045231461525,
0.07685143500566483,
-0.0011010454036295414,
0.05012333020567894,
0.016921671107411385,
-0.06701025366783142,
-0.0014603501185774803,
-0.03541533648967743,
0.016401784494519234,
-0.0196052398532629,
0.1686866283416748,
-0.034576330333948135,
0.020211992785334587,
0.10293343663215637,
0.021409381181001663,
0.05230940878391266,
-0.005569851957261562,
-0.014118647202849388,
-0.024366039782762527,
-0.0048927003517746925,
0.06265802681446075,
-0.04844793304800987,
0.007035724353045225,
0.06609053164720535,
-0.028727293014526367,
-0.01761632226407528,
-0.018108194693922997,
-0.006405983120203018,
0.019953101873397827,
0.0006767826271243393,
-0.03913893550634384,
-0.0019858924206346273,
0.06005441024899483,
-0.058446478098630905,
-0.06149250641465187,
-0.005203471519052982,
0.003692285856232047,
0.04953918606042862,
-0.021843677386641502,
0.0026094538625329733,
0.03878605365753174,
0.04196041822433472,
0.02425450272858143,
-0.027291152626276016,
-0.022694088518619537,
-0.02894517593085766,
-0.030954744666814804,
-0.016432512551546097,
0.034721504896879196,
0.06171230226755142,
-0.03067978098988533,
0.08706250041723251,
0.023792095482349396
] |
PMID:27171 | Transient kinetics of nicotinamide-adenine dinucleotide phosphate-linked isocitrate dehydrogenase from bovine heart mitochondria. | Pre-steady-state studies of the isocitrate dehydrogenase reaction show that the rate constant for the hydride-transfer step is above 990s-1, and that both subunits of the enzyme are simulataneously active. After the fast formation of NADPH in amounts equivalent to the enzyme subunit concentration, the rate of NADPH formation is equal to the steady-state rate if the enzyme has been preincubated with isocitrate and Mg2+. If the enzyme has been preincubated with NADP+ and Mg2+, in 0.05 M-triethanolamine chloride buffer, pH 7.0, with the addition of 0.1 M-NaCl, the amount of NADPH formed in the fast phase is only 60% of the enzyme subunit concentration, and the turnover rate is at first lower than the steady-state rate. In 0.05 M-triethanolamine chloride buffer, pH 7.0, if the enzyme is preincubated with NADP+ or NADPH, the turnover rate increases 3-fold to reach the steady-state rate after about 5 s. Preincubation of the enzyme with isocitrate and Mg2+ abolishes this lag phase, the steady-state rate being reached at once. It is suggested that the enzyme exists in at least two conformational forms with different activities, and that the lag phase represents the transition (k = 0.4s-1) from a form with low activity to the fully active enzyme, induced by the binding of isocitrate and Mg2+. | Transient kinetics of nicotinamide-adenine dinucleotide phosphate-linked isocitrate dehydrogenase from bovine heart mitochondria. Pre-steady-state studies of the isocitrate dehydrogenase reaction show that the rate constant for the hydride-transfer step is above 990s-1, and that both subunits of the enzyme are simulataneously active. After the fast formation of NADPH in amounts equivalent to the enzyme subunit concentration, the rate of NADPH formation is equal to the steady-state rate if the enzyme has been preincubated with isocitrate and Mg2+. If the enzyme has been preincubated with NADP+ and Mg2+, in 0.05 M-triethanolamine chloride buffer, pH 7.0, with the addition of 0.1 M-NaCl, the amount of NADPH formed in the fast phase is only 60% of the enzyme subunit concentration, and the turnover rate is at first lower than the steady-state rate. In 0.05 M-triethanolamine chloride buffer, pH 7.0, if the enzyme is preincubated with NADP+ or NADPH, the turnover rate increases 3-fold to reach the steady-state rate after about 5 s. Preincubation of the enzyme with isocitrate and Mg2+ abolishes this lag phase, the steady-state rate being reached at once. It is suggested that the enzyme exists in at least two conformational forms with different activities, and that the lag phase represents the transition (k = 0.4s-1) from a form with low activity to the fully active enzyme, induced by the binding of isocitrate and Mg2+. | [
-0.08308351039886475,
0.03158944845199585,
-0.05009281262755394,
0.0646059662103653,
0.03936855122447014,
0.03739554435014725,
0.030436484143137932,
-0.009722815826535225,
0.011873404495418072,
-0.015243381261825562,
0.03905513137578964,
-0.06785321980714798,
0.01955295167863369,
0.029443284496665,
-0.04040509834885597,
0.008198858238756657,
0.004910309799015522,
-0.019139235839247704,
0.00501367449760437,
-0.04859840124845505,
-0.0009433361701667309,
-0.012195480987429619,
0.04228350520133972,
-0.0022154860198497772,
0.057347338646650314,
0.04065471142530441,
0.018338801339268684,
0.015548137947916985,
-0.07956873625516891,
-0.23358510434627533,
0.02822566032409668,
-0.011487136594951153,
-0.02003101259469986,
-0.04810235649347305,
-0.01335532683879137,
0.03268817812204361,
-0.003045756369829178,
0.04343237727880478,
-0.009753555059432983,
0.044595520943403244,
0.021340807899832726,
0.025876794010400772,
0.017049992457032204,
0.005812902003526688,
-0.011565431952476501,
-0.025753851979970932,
-0.036366697400808334,
0.002052030060440302,
0.08478657156229019,
-0.020195288583636284,
-0.02615884318947792,
0.020110098645091057,
-0.01105501689016819,
-0.035661421716213226,
0.038099631667137146,
0.0008241422474384308,
0.040569573640823364,
0.06059395521879196,
-0.001887587714008987,
-0.02496739663183689,
0.01268258411437273,
0.004843637812882662,
-0.11644505709409714,
0.031336333602666855,
0.05401611328125,
0.013160794042050838,
-0.0010498936753720045,
-0.04506371170282364,
0.021912531927227974,
-0.004786356817930937,
-0.10699399560689926,
0.04016009718179703,
0.017205722630023956,
-0.06348700821399689,
0.013790289871394634,
-0.03336915001273155,
0.024943318217992783,
0.02250419743359089,
0.049156978726387024,
-0.00728299655020237,
-0.011312399059534073,
-0.009841184131801128,
-0.020429175347089767,
-0.035615917295217514,
-0.005999408196657896,
0.0351228341460228,
0.07255849242210388,
-0.0358724519610405,
0.005793644580990076,
0.030160458758473396,
0.009675225242972374,
-0.03777957335114479,
0.026216376572847366,
-0.01004739385098219,
-0.07647272944450378,
0.0794534757733345,
0.043363362550735474,
0.04076458513736725,
0.0018419111147522926,
0.3421157896518707,
-0.05803542584180832,
0.07335413992404938,
-0.013911568559706211,
-0.05877945199608803,
-0.008593403734266758,
-0.04491223767399788,
-0.023073822259902954,
0.030483810231089592,
0.019435031339526176,
0.028746457770466805,
0.025011848658323288,
0.010946858674287796,
0.007996765896677971,
-0.09684625267982483,
0.02246246114373207,
0.013426006771624088,
-0.03185678645968437,
0.0028635640628635883,
-0.03801046684384346,
0.009939271956682205,
0.029685698449611664,
0.08562823385000229,
0.0017303579952567816,
-0.004522179253399372,
0.042074836790561676,
0.015132145956158638,
-0.024026107043027878,
0.09119179844856262,
0.08930995315313339,
-0.004138789139688015,
0.0774940624833107,
0.04439038783311844,
-0.03407468646764755,
-0.01638220250606537,
-0.03294441103935242,
-0.003454238874837756,
0.024159839376807213,
-0.024041414260864258,
0.055301375687122345,
-0.013840417377650738,
-0.012151489034295082,
-0.0362866036593914,
-0.04400601610541344,
-0.0608738474547863,
-0.05309576168656349,
0.07295464724302292,
0.008219733834266663,
0.06560276448726654,
-0.0457381047308445,
0.0020514396019279957,
0.021209988743066788,
0.06148936226963997,
-0.025840096175670624,
0.000017042335457517765,
-0.04052887111902237,
-0.001497650402598083,
-0.009822065010666847,
-0.010640311986207962,
0.03263799846172333,
-0.03254216909408569,
-0.16684690117835999,
-0.02537400647997856,
0.021062495186924934,
0.07056215405464172,
-0.014691608026623726,
0.017729049548506737,
-0.014404923655092716,
-0.025874339044094086,
0.0031023253686726093,
-0.03950226679444313,
-0.014511260204017162,
0.013478321023285389,
0.009496322833001614,
-0.02812412939965725,
0.009485029615461826,
0.017210273072123528,
-0.00028527906397357583,
0.007428554352372885,
0.017601164057850838,
-0.02249445766210556,
0.03359612450003624,
-0.0033613049890846014,
-0.018117062747478485,
0.039971135556697845,
0.04432974383234978,
0.010998135432600975,
-0.059573832899332047,
0.021447064355015755,
-0.0038633905351161957,
0.07876751571893692,
-0.03608505800366402,
-0.018567901104688644,
-0.1041092723608017,
0.04603080824017525,
-0.07584276050329208,
-0.0628863126039505,
-0.036946725100278854,
-0.020295795053243637,
0.025898221880197525,
-0.0393964946269989,
0.11395087838172913,
-0.0120848398655653,
0.011970279738307,
0.06696628034114838,
-0.026571866124868393,
0.023327240720391273,
-0.03729919344186783,
0.07072973996400833,
0.02743900753557682,
0.07664163410663605,
-0.0649363100528717,
-0.0422203354537487,
-0.06651236861944199,
-0.005102531518787146,
-0.08564594388008118,
0.04620477929711342,
-0.02919062227010727,
0.04771851375699043,
0.051826704293489456,
0.03969782218337059,
-0.013104623183608055,
-0.05636488273739815,
-0.016937144100666046,
-0.28985604643821716,
-0.010643825866281986,
0.05026930943131447,
-0.027798358350992203,
0.08727164566516876,
-0.0256429985165596,
0.04909181967377663,
-0.03253040090203285,
0.0025352295488119125,
-0.039536040276288986,
0.030619213357567787,
0.02711225301027298,
0.0037018219009041786,
-0.07304331660270691,
-0.050150398164987564,
0.060583386570215225,
0.02045668289065361,
-0.018797609955072403,
0.050503622740507126,
-0.018630769103765488,
0.04196774587035179,
-0.03059292770922184,
-0.022079631686210632,
0.009774374775588512,
0.040122609585523605,
-0.023919235914945602,
0.07336467504501343,
-0.055125437676906586,
0.0008822539821267128,
-0.009067234583199024,
-0.005890189670026302,
0.01816241256892681,
-0.06126382201910019,
-0.05229267105460167,
-0.07440585643053055,
-0.035402171313762665,
0.019345905631780624,
-0.04726991802453995,
0.04626569524407387,
-0.00710555724799633,
-0.0030168609227985144,
0.0434548519551754,
-0.0026615974493324757,
-0.03644432872533798,
-0.009482745081186295,
0.004305100068449974,
0.00960925780236721,
-0.016217734664678574,
0.01232913602143526,
-0.02789483591914177,
0.054755765944719315,
-0.0273731779307127,
0.03747669979929924,
-0.09712592512369156,
0.012011614628136158,
-0.012373735196888447,
-0.05423766002058983,
-0.035897646099328995,
-0.05456085875630379,
0.020217860117554665,
-0.027373531833291054,
0.014987179078161716,
-0.02737409621477127,
0.03116910718381405,
-0.0026271650567650795,
-0.028626365587115288,
-0.022268913686275482,
0.009680677205324173,
0.023889150470495224,
-0.03381256386637688,
0.05326429381966591,
0.08888494223356247,
-0.03551304712891579,
-0.032352641224861145,
0.031668126583099365,
-0.00878711137920618,
-0.0968502089381218,
-0.03699308633804321,
-0.04627670720219612,
0.04619510844349861,
-0.019819164648652077,
-0.048049669712781906,
0.1048869788646698,
-0.013130375184118748,
0.014694717712700367,
0.014345918782055378,
0.06457984447479248,
-0.041527993977069855,
-0.012917427346110344,
0.0002696029550861567,
0.024127939715981483,
-0.0006843024748377502,
-0.0032969871535897255,
-0.009675330482423306,
-0.0313262939453125,
0.06471150368452072,
-0.27128615975379944,
-0.044592805206775665,
0.012137165293097496,
0.010066788643598557,
0.01654326543211937,
0.056093841791152954,
0.07497907429933548,
-0.028585882857441902,
-0.026672733947634697,
0.05075109377503395,
-0.02298007905483246,
0.04535286873579025,
0.06977637857198715,
0.09054483473300934,
-0.0016484586521983147,
0.03347678855061531,
0.021682819351553917,
-0.050848186016082764,
-0.014381809160113335,
-0.05806824192404747,
-0.005719001870602369,
-0.0501379519701004,
0.15400496125221252,
-0.0028410088270902634,
0.01687333919107914,
0.08052565157413483,
0.030205175280570984,
0.041270662099123,
0.016905415803194046,
-0.007395257707685232,
-0.04417746886610985,
0.030061041936278343,
0.06710883975028992,
-0.02054585888981819,
0.0024440521374344826,
0.03537856787443161,
-0.01545135211199522,
0.004104298073798418,
-0.022661395370960236,
0.005795437842607498,
0.01006079651415348,
0.01152832992374897,
-0.006666917819529772,
-0.016383463516831398,
0.0475304089486599,
-0.0647224634885788,
-0.05813794955611229,
-0.05191681161522865,
-0.04941026493906975,
0.08140189945697784,
0.007550837937742472,
0.003917217720299959,
0.04121396690607071,
0.034922268241643906,
0.02735719457268715,
-0.00346971582621336,
-0.013110858388245106,
-0.02346011996269226,
-0.02475663274526596,
-0.021819448098540306,
0.022305089980363846,
0.03186418488621712,
-0.01303589716553688,
0.07831241190433502,
0.03436201438307762
] |
PMID:27172 | Purification and some properties of an alkaline proteinase from rat skeletal muscle. | 1. Rat skeletal muscle was homogenized in 0.05M-Tris/HCl, pH 8.5, containing 1M-KCl. Myofibrillar proteins were precipitated by addition of (NH4)2SO4 (33% saturation). 2. The alkaline proteolytic activity that was precipitated with the myofibrillar proteins was solubilized with trypsin (conjugated to Sepharose) and further purified by affinity chromatography, ion-exchange chromatography and gel filtration. 3. The purified enzyme migrates as a single band in polyacrylamide-disc electrophoresis, and has optimum hydrolytic activity with azocasein and [14C]haemoglobin as substrates at pH 9.4 and 9.6 respectively. Its apparent molecular weight, as determined by gel filtration on Sephadex G-75, is 30800. 4. The purified alkaline proteinase is strongly inhibited by equimolar amounts of soya-bean trypsin inhibitor and ovomucoid, whereas di-isopropyl phosphorofluoidate and alpha-toluenesulphonyl fluoride have no effect. On the other hand N-ethylmaleimide and p-chloromercuribenzoate have inhibitory effects on the enzyme activity. 5. Bivalent metal ions (Fe2+, Co2+, Zn2+, Mg2+, Mn2+) diminish the proteolytic activity, at 1mM concentrations. Ca2+ ions and the metal-ion-chelating agent EDTA are without effect on enzyme activity. 6. The enzyme is part of the alkaline proteolytic activity that appears to be associated with myofibrillar proteins. | Purification and some properties of an alkaline proteinase from rat skeletal muscle. 1. Rat skeletal muscle was homogenized in 0.05M-Tris/HCl, pH 8.5, containing 1M-KCl. Myofibrillar proteins were precipitated by addition of (NH4)2SO4 (33% saturation). 2. The alkaline proteolytic activity that was precipitated with the myofibrillar proteins was solubilized with trypsin (conjugated to Sepharose) and further purified by affinity chromatography, ion-exchange chromatography and gel filtration. 3. The purified enzyme migrates as a single band in polyacrylamide-disc electrophoresis, and has optimum hydrolytic activity with azocasein and [14C]haemoglobin as substrates at pH 9.4 and 9.6 respectively. Its apparent molecular weight, as determined by gel filtration on Sephadex G-75, is 30800. 4. The purified alkaline proteinase is strongly inhibited by equimolar amounts of soya-bean trypsin inhibitor and ovomucoid, whereas di-isopropyl phosphorofluoidate and alpha-toluenesulphonyl fluoride have no effect. On the other hand N-ethylmaleimide and p-chloromercuribenzoate have inhibitory effects on the enzyme activity. 5. Bivalent metal ions (Fe2+, Co2+, Zn2+, Mg2+, Mn2+) diminish the proteolytic activity, at 1mM concentrations. Ca2+ ions and the metal-ion-chelating agent EDTA are without effect on enzyme activity. 6. The enzyme is part of the alkaline proteolytic activity that appears to be associated with myofibrillar proteins. | [
-0.08227051794528961,
-0.050364892929792404,
-0.04303556680679321,
-0.034848652780056,
0.002905613277107477,
-0.08879998326301575,
0.0734693631529808,
0.020993847399950027,
-0.05337400734424591,
-0.015354465693235397,
0.009768718853592873,
-0.07602152228355408,
-0.028286701068282127,
0.04015950486063957,
-0.002202461939305067,
-0.041361428797245026,
0.02105512097477913,
0.019238246604800224,
-0.06789744645357132,
-0.03306044638156891,
-0.022530829533934593,
0.02578670345246792,
0.06794309616088867,
-0.0010449521942064166,
0.07241266965866089,
0.0356779620051384,
-0.013365589082241058,
0.019926417618989944,
-0.016455359756946564,
-0.18267568945884705,
-0.01113884150981903,
-0.06700567901134491,
0.05992446839809418,
-0.056150685995817184,
-0.01772337220609188,
0.04572807624936104,
0.018055886030197144,
-0.0015843287110328674,
-0.049661848694086075,
0.05745714157819748,
0.0580703429877758,
0.005627314560115337,
0.0004731538356281817,
0.0440070703625679,
0.05313066020607948,
0.041020285338163376,
-0.002950694179162383,
-0.0010128754656761885,
0.09728486090898514,
0.05804707482457161,
-0.02143670804798603,
0.021161867305636406,
0.06511620432138443,
0.02878568135201931,
0.04200683906674385,
0.018568428233265877,
0.035311415791511536,
0.06909091770648956,
-0.05962550267577171,
0.017513718456029892,
0.024782812222838402,
0.03811362758278847,
-0.11386039853096008,
0.04592946544289589,
0.01098675187677145,
0.06607282161712646,
0.022819608449935913,
0.0010389545932412148,
0.03221666440367699,
0.06148277223110199,
-0.030695538967847824,
0.11168160289525986,
0.04977746680378914,
0.03758970648050308,
0.025698047131299973,
-0.03881875053048134,
-0.0037156620528548956,
0.04517779499292374,
0.04904720187187195,
0.03623922914266586,
-0.0318300798535347,
-0.007846331223845482,
0.013610095717012882,
-0.0014989750925451517,
-0.03901449963450432,
0.06372782588005066,
0.035297371447086334,
0.005246741697192192,
0.0012613877188414335,
0.020229678601026535,
-0.02909170091152191,
-0.018247274681925774,
0.043784406036138535,
-0.06481462717056274,
-0.08815141767263412,
0.09146172553300858,
0.0014599654823541641,
0.03029572404921055,
-0.0028318949043750763,
0.3405624032020569,
-0.0165358055382967,
0.028455521911382675,
-0.01818145252764225,
-0.002497732639312744,
-0.023467538878321648,
-0.017849460244178772,
-0.032428838312625885,
0.050741735845804214,
-0.02161312848329544,
0.050042640417814255,
0.03399733826518059,
0.023979106917977333,
-0.03355610370635986,
-0.023021096363663673,
0.014527956955134869,
-0.016251785680651665,
0.03671439364552498,
0.010809100233018398,
0.007209592964500189,
0.05788048356771469,
-0.09870929270982742,
-0.00832004938274622,
0.027925241738557816,
0.017200764268636703,
0.02127409167587757,
0.006396481767296791,
-0.020009823143482208,
0.10676895081996918,
0.09260019659996033,
-0.019894709810614586,
0.00513008888810873,
0.03419681638479233,
-0.03405133634805679,
0.004037453792989254,
0.032646894454956055,
-0.017208850011229515,
-0.0008170881774276495,
0.003966616932302713,
-0.013818331062793732,
-0.007660457398742437,
-0.03156440332531929,
-0.037142422050237656,
-0.09768213331699371,
-0.06593810021877289,
-0.04373389482498169,
0.03731817752122879,
-0.002309308387339115,
0.04478008672595024,
-0.02162022702395916,
-0.0453934408724308,
0.022429952397942543,
0.008897922933101654,
-0.0173373743891716,
0.05281888321042061,
-0.01955432817339897,
0.021258950233459473,
0.009470019489526749,
-0.04575755447149277,
0.028010420501232147,
-0.013618537224829197,
-0.10307835787534714,
-0.03576211258769035,
0.0210020262748003,
0.004687228240072727,
0.02387043833732605,
0.004891097545623779,
-0.06502389907836914,
0.02261301316320896,
-0.006799007300287485,
-0.03628845140337944,
-0.006138589233160019,
0.022264843806624413,
-0.08423708379268646,
-0.015429126098752022,
-0.037461522966623306,
0.029271665960550308,
-0.020904500037431717,
-0.02206188626587391,
0.015827974304556847,
-0.04362177476286888,
0.031071364879608154,
-0.03142593428492546,
0.0036437101662158966,
0.03452224284410477,
0.031284231692552567,
-0.020767053589224815,
-0.04655522480607033,
0.04100996255874634,
0.0157148614525795,
0.06278251111507416,
0.004698931705206633,
-0.006589946802705526,
-0.09575459361076355,
0.027839941903948784,
-0.06261488050222397,
-0.05143681541085243,
-0.056944821029901505,
0.038160230964422226,
0.006558865308761597,
-0.06929033249616623,
0.1311815083026886,
0.0006924450281076133,
0.0012555260909721255,
0.02939499542117119,
-0.02573910355567932,
0.06376738101243973,
-0.04426603764295578,
0.055304333567619324,
0.026095891371369362,
0.030898775905370712,
-0.02713177353143692,
0.03171003237366676,
-0.08429963141679764,
-0.014744717627763748,
-0.020043086260557175,
0.04218496382236481,
-0.012002267874777317,
-0.010000381618738174,
0.03881102800369263,
-0.01724301464855671,
-0.004181194584816694,
-0.06664575636386871,
-0.0663556307554245,
-0.2639356553554535,
0.014285312965512276,
0.008732416667044163,
-0.0013644270366057754,
0.06856926530599594,
-0.05580301210284233,
0.013000736944377422,
-0.03219858184456825,
0.013543405570089817,
-0.07630865275859833,
0.0374440960586071,
0.02699202485382557,
-0.04001355543732643,
-0.021155504509806633,
0.011885497719049454,
0.04101882874965668,
0.006025972776114941,
-0.02221536636352539,
-0.029377607628703117,
-0.014155278913676739,
0.03635720908641815,
-0.01813252456486225,
0.006505606230348349,
0.061589423567056656,
0.07826460152864456,
-0.01685904711484909,
0.06821728497743607,
0.023564854636788368,
0.007073166314512491,
-0.04982473701238632,
0.007138645276427269,
0.08052561432123184,
-0.04061121866106987,
-0.10376632213592529,
-0.021439485251903534,
-0.05488311126828194,
-0.025205275043845177,
-0.030714528635144234,
0.01534939929842949,
-0.01835666410624981,
-0.06957250833511353,
0.022803885862231255,
0.020155606791377068,
-0.048100534826517105,
-0.03730306029319763,
-0.020411090925335884,
0.04479089006781578,
0.020053086802363396,
-0.0014965777518227696,
0.01567179709672928,
0.03480377793312073,
-0.01933804526925087,
0.02513294853270054,
-0.035815510898828506,
0.004099423065781593,
-0.038735050708055496,
-0.023011885583400726,
0.04118770733475685,
-0.06272347271442413,
-0.023305969312787056,
0.005612694192677736,
-0.030791737139225006,
-0.019036514684557915,
0.05562194809317589,
0.02067556045949459,
-0.013929001986980438,
0.028697405010461807,
0.012035931460559368,
0.015252122655510902,
0.004014979116618633,
0.007732733152806759,
0.09398991614580154,
-0.0140632139518857,
0.005031682085245848,
0.11023972183465958,
0.047503575682640076,
-0.0257156603038311,
-0.02750331722199917,
-0.01276428159326315,
-0.01041324995458126,
-0.023601531982421875,
-0.03253979608416557,
0.056041426956653595,
-0.046879541128873825,
-0.0017608451889827847,
0.05254361778497696,
0.04153960943222046,
-0.003801195416599512,
0.03492414578795433,
0.032333459705114365,
0.058372702449560165,
-0.009481754153966904,
-0.05023116618394852,
0.005476596299558878,
0.03928697854280472,
-0.029872221872210503,
-0.2826839089393616,
-0.038580942898988724,
0.06817466765642166,
-0.009997098706662655,
-0.019050968810915947,
0.002949685789644718,
0.039640069007873535,
-0.06740805506706238,
-0.0006825616001151502,
0.07967961579561234,
-0.03130444884300232,
0.031062349677085876,
0.026614943519234657,
0.015159988775849342,
-0.036121759563684464,
0.04910392314195633,
0.04327014461159706,
-0.022369230166077614,
0.0787402093410492,
-0.08083893358707428,
0.0022706124000251293,
-0.09714274108409882,
0.13683132827281952,
-0.009094182401895523,
0.020793702453374863,
0.07981773465871811,
0.03373713791370392,
-0.018158219754695892,
-0.03459750860929489,
-0.02625603787600994,
0.019810836762189865,
0.028038160875439644,
0.10045472532510757,
-0.026127932593226433,
-0.023020511493086815,
0.0015361544210463762,
0.005596664268523455,
-0.026201674714684486,
-0.02556908316910267,
-0.009065675549209118,
-0.004320772364735603,
-0.0002759987546596676,
-0.09930473566055298,
-0.052889060229063034,
0.028304072096943855,
-0.10749683529138565,
-0.07297728955745697,
-0.031092505902051926,
-0.012096324004232883,
0.013589486479759216,
0.005284412764012814,
-0.03376079350709915,
0.037148553878068924,
0.0035203942097723484,
-0.0070713115856051445,
-0.03288843110203743,
-0.0024649419356137514,
0.00010373550321673974,
-0.05462910234928131,
-0.03208918869495392,
0.040417056530714035,
0.01760655641555786,
-0.05454103648662567,
0.06650687754154205,
0.03913029283285141
] |
PMID:27198 | Association of defective feedback suppressor T cell activity with autoimmunity in NZB mice. | A "feedback suppressor T cell" highly dependent on signals from Ly 1 T helper cells for activation is described. The signal from the Ly 1 cell binds to Fc-like receptors on macrophage membranes. The feedback suppressor cell expresses all three Ly antigens as well as the Qa 1 antigen on its surface, is very sensitive to low doses of cyclophosphamide, disappears relatively rapidly after adult thymectomy, and cannot be demonstrated in NZB mice 6 weeks or older. | Association of defective feedback suppressor T cell activity with autoimmunity in NZB mice. A "feedback suppressor T cell" highly dependent on signals from Ly 1 T helper cells for activation is described. The signal from the Ly 1 cell binds to Fc-like receptors on macrophage membranes. The feedback suppressor cell expresses all three Ly antigens as well as the Qa 1 antigen on its surface, is very sensitive to low doses of cyclophosphamide, disappears relatively rapidly after adult thymectomy, and cannot be demonstrated in NZB mice 6 weeks or older. | [
-0.041267767548561096,
0.007172626443207264,
-0.019672047346830368,
0.04947911202907562,
-0.051097892224788666,
-0.018845820799469948,
0.09122079610824585,
0.0934562087059021,
-0.0482250340282917,
-0.026843126863241196,
0.025417717173695564,
-0.06225347891449928,
0.04325440153479576,
0.03310848027467728,
-0.0050543067045509815,
-0.01095796562731266,
0.0012493530521169305,
-0.03551097959280014,
-0.04931606352329254,
0.002785530872642994,
-0.051967378705739975,
-0.05519849434494972,
0.04717501625418663,
-0.00223609060049057,
0.028456082567572594,
-0.011077305302023888,
0.0004060477949678898,
-0.014371762983500957,
-0.040385741740465164,
-0.1773613840341568,
-0.004267812240868807,
-0.003934770356863737,
-0.007622327655553818,
0.03491919860243797,
-0.0033640179317444563,
0.017747776582837105,
-0.04018473997712135,
-0.06224567070603371,
-0.029657918959856033,
0.023985635489225388,
0.04888592287898064,
0.04169395565986633,
0.036897577345371246,
0.012999053113162518,
0.07838720828294754,
-0.02026483230292797,
-0.02685130573809147,
-0.02807597629725933,
0.013297230936586857,
0.02006121352314949,
0.041075199842453,
0.006985207553952932,
0.029108194634318352,
0.040440380573272705,
0.015786930918693542,
0.0014226712519302964,
-0.019744599238038063,
0.07096287608146667,
0.04722237586975098,
0.07564229518175125,
0.01449570618569851,
-0.007189028896391392,
-0.11316974461078644,
0.06244967132806778,
0.10097452998161316,
-0.021755196154117584,
0.005605567712336779,
-0.017434172332286835,
0.016193058341741562,
0.026261508464813232,
-0.049716442823410034,
-0.0001689711061771959,
-0.005127024371176958,
0.009032152593135834,
-0.005606818478554487,
-0.009845945984125137,
-0.048729509115219116,
0.008460446260869503,
0.06284039467573166,
0.02822374552488327,
0.006993455346673727,
-0.011881831102073193,
-0.02086527831852436,
0.00035883134114556015,
-0.051386624574661255,
-0.05168157070875168,
-0.0036197283770889044,
-0.10617509484291077,
0.015952130779623985,
0.03194568306207657,
-0.02604474127292633,
-0.060483112931251526,
0.056596122682094574,
0.005616870243102312,
-0.07272856682538986,
-0.020376071333885193,
0.012931772507727146,
-0.03319067880511284,
0.005636994261294603,
0.4138719141483307,
-0.023051057010889053,
-0.01635260507464409,
-0.05348505079746246,
0.03971632570028305,
0.0014573851367458701,
-0.02731882408261299,
0.04470745101571083,
0.0058134919963777065,
-0.008946580812335014,
0.06082908436655998,
0.04839714989066124,
-0.029729748144745827,
0.02580331079661846,
-0.026757800951600075,
0.007758188061416149,
-0.01504337415099144,
0.043777819722890854,
0.047114066779613495,
0.008390980772674084,
0.00212052371352911,
0.03687957301735878,
-0.020863179117441177,
0.05473830923438072,
-0.05047840252518654,
0.07479093223810196,
0.08254928141832352,
0.018829934298992157,
0.06423887610435486,
0.0428730733692646,
-0.014782707206904888,
0.041270069777965546,
-0.031695254147052765,
-0.02092784456908703,
0.009150560013949871,
0.00762662198394537,
-0.011172128841280937,
-0.009090130217373371,
-0.060412563383579254,
-0.027487164363265038,
-0.01774721033871174,
0.04555472731590271,
-0.07393224537372589,
0.019060205668210983,
-0.06517684459686279,
-0.019749773666262627,
0.019928257912397385,
-0.03953848034143448,
-0.02226090431213379,
-0.005410598125308752,
0.031734250485897064,
-0.025989338755607605,
0.06860162317752838,
-0.032586369663476944,
0.03550777956843376,
-0.014727597124874592,
0.06606948375701904,
-0.043773550540208817,
-0.014234679751098156,
0.049390703439712524,
-0.015164518728852272,
-0.07523290067911148,
-0.07524483650922775,
0.01677212491631508,
-0.0062063755467534065,
-0.030020978301763535,
-0.00798831507563591,
-0.023214122280478477,
-0.020217392593622208,
0.021688491106033325,
-0.008710624650120735,
-0.0027859604451805353,
0.041000764816999435,
-0.06192885711789131,
-0.04281771183013916,
-0.04286450147628784,
0.005932325031608343,
-0.062155354768037796,
-0.0005810248549096286,
0.020845623686909676,
0.011833456344902515,
-0.05025001987814903,
-0.05785743147134781,
0.02934711053967476,
-0.03405008465051651,
-0.02950705774128437,
0.029395874589681625,
-0.015165094286203384,
-0.005039514973759651,
-0.00493711419403553,
-0.00398726761341095,
0.004036696627736092,
-0.07736718654632568,
-0.06827032566070557,
-0.05961833894252777,
-0.040041495114564896,
-0.03009386546909809,
-0.0157989002764225,
-0.037596214562654495,
0.012818751856684685,
-0.0537879653275013,
0.08150647580623627,
-0.013397997245192528,
0.02210162580013275,
0.07444719225168228,
0.03508947417140007,
0.04081170633435249,
0.03308575227856636,
-0.017118703573942184,
0.10688512772321701,
-0.038122303783893585,
-0.01993597112596035,
-0.010100194253027439,
0.004176991526037455,
0.01678461581468582,
0.003472876502200961,
0.027565931901335716,
0.00533211138099432,
-0.05229949578642845,
0.04390953481197357,
-0.00411989726126194,
0.09976384043693542,
0.048201918601989746,
-0.07959441840648651,
-0.28308483958244324,
-0.0709877535700798,
-0.0036352472379803658,
0.0024391745682805777,
-0.039504241198301315,
-0.05565530061721802,
-0.01989690400660038,
-0.0018155627185478806,
0.015625542029738426,
-0.0031413740944117308,
0.05328768864274025,
0.13025884330272675,
-0.02565024234354496,
-0.038969121873378754,
0.0026335723232477903,
0.08868728578090668,
-0.012334478087723255,
0.036521609872579575,
0.014972994104027748,
0.020557254552841187,
-0.07320316135883331,
0.0222222488373518,
0.007115315180271864,
0.024934004992246628,
0.02777879498898983,
-0.003594106761738658,
0.14338092505931854,
0.025649281218647957,
0.008668766357004642,
0.033613137900829315,
0.04448864609003067,
0.01712530106306076,
-0.019308025017380714,
0.0028827216010540724,
-0.01799219846725464,
-0.00994863361120224,
-0.03335067629814148,
-0.024178212508559227,
-0.0030571192037314177,
-0.004413365386426449,
-0.016500212252140045,
0.0013306336477398872,
-0.04800791293382645,
-0.0612330324947834,
-0.048398781567811966,
0.037355687469244,
-0.0010999233927577734,
0.11891602724790573,
-0.046431589871644974,
-0.021008003503084183,
0.03740459680557251,
-0.05708623304963112,
0.006426802836358547,
0.04496736079454422,
0.012077332474291325,
0.04242141917347908,
-0.026981530711054802,
0.0030408119782805443,
-0.05456585809588432,
0.026090439409017563,
-0.012247741222381592,
0.040018126368522644,
0.01937534660100937,
-0.018739286810159683,
-0.021206920966506004,
0.009093266911804676,
0.029694639146327972,
0.005868602078408003,
0.03772920370101929,
0.013703134842216969,
-0.0029727681539952755,
0.0742795541882515,
0.0165298730134964,
0.042865216732025146,
0.0405673049390316,
0.013419284485280514,
-0.04184452444314957,
-0.03786112368106842,
-0.08187355101108551,
0.029600149020552635,
0.045562494546175,
-0.03605524078011513,
0.06055944785475731,
0.03257792815566063,
0.020774370059370995,
-0.00004755164627567865,
0.015914995223283768,
0.029909230768680573,
0.031259551644325256,
0.027605954557657242,
-0.03710521385073662,
0.00607838062569499,
-0.017000721767544746,
-0.0771152600646019,
0.009599060751497746,
-0.04545119032263756,
-0.2686457633972168,
-0.03353922814130783,
0.03647594153881073,
0.0350433811545372,
-0.04897882789373398,
0.015819430351257324,
-0.005397313740104437,
-0.05293925851583481,
-0.05960853025317192,
0.04841494560241699,
0.01087202224880457,
0.023196179419755936,
0.02105359174311161,
0.03450595587491989,
-0.01233107689768076,
-0.01628323830664158,
0.03380787745118141,
-0.0726478323340416,
0.004643974360078573,
-0.0023911925964057446,
0.009772151708602905,
-0.051404550671577454,
0.14374208450317383,
0.08139960467815399,
0.015916433185338974,
0.04544856771826744,
-0.019162282347679138,
0.0029851545114070177,
-0.03825649246573448,
-0.05529630184173584,
-0.02187546156346798,
-0.01447376236319542,
0.07407499849796295,
0.024567417800426483,
-0.01841798610985279,
0.03129350394010544,
-0.061379704624414444,
0.04411321505904198,
-0.022748706862330437,
0.03430354967713356,
-0.03071865253150463,
0.05695118010044098,
-0.03695042431354523,
-0.04552382975816727,
0.11401065438985825,
-0.0363524928689003,
-0.0410267636179924,
-0.03468119725584984,
-0.018886320292949677,
0.010301323607563972,
-0.02794034406542778,
0.009435744024813175,
-0.005678542423993349,
-0.02960391528904438,
-0.0192806888371706,
-0.048016469925642014,
-0.0003132788697257638,
-0.015333040617406368,
0.02604612708091736,
0.0234496109187603,
0.030656861141324043,
-0.00439473707228899,
0.010396935045719147,
0.06587917357683182,
0.05171864479780197
] |
PMID:27203 | Some clinical pharmacological studies with terfenadine, a new antihistamine drug. | 1 A double-blind cross-over trial between placebo, chlorpheniramine, and terfenadine, a new antihistamine drug, was performed in healthy male volunteers to determine and compare their CNS and autonomic effects. 2 Terfenadine and chlorpheniramine were administered orally in therapeutic doses. 3 In objective tests of critical flicker frequency, pursuit rotor, reaction time, salivary volume and pupillary diameter, no statistically significant difference was observed between the treatments. 4 On analogue rating scales, chlorpheniramine produced a statistically significant (P less than 0.05) degree of sedation and impaired concentration as compared to placebo and terfenadine. 5 The results obtained in analogue rating scales were not normally distributed and, therefore, use of non-parametric statistical methods for analysis of such data is strongly advocated. | Some clinical pharmacological studies with terfenadine, a new antihistamine drug. 1 A double-blind cross-over trial between placebo, chlorpheniramine, and terfenadine, a new antihistamine drug, was performed in healthy male volunteers to determine and compare their CNS and autonomic effects. 2 Terfenadine and chlorpheniramine were administered orally in therapeutic doses. 3 In objective tests of critical flicker frequency, pursuit rotor, reaction time, salivary volume and pupillary diameter, no statistically significant difference was observed between the treatments. 4 On analogue rating scales, chlorpheniramine produced a statistically significant (P less than 0.05) degree of sedation and impaired concentration as compared to placebo and terfenadine. 5 The results obtained in analogue rating scales were not normally distributed and, therefore, use of non-parametric statistical methods for analysis of such data is strongly advocated. | [
-0.023276738822460175,
-0.013793633319437504,
-0.020834721624851227,
0.0450432188808918,
0.003930424805730581,
0.010722270235419273,
0.09897500276565552,
0.049741148948669434,
-0.061857618391513824,
-0.054892875254154205,
0.03078816644847393,
0.008717989549040794,
0.022266337648034096,
0.040080517530441284,
0.014162763953208923,
-0.012136658653616905,
0.015985162928700447,
-0.03313026949763298,
0.009106224402785301,
-0.035358745604753494,
-0.06475173681974411,
0.03465675562620163,
0.08454454690217972,
-0.0048467135056853294,
-0.014658452942967415,
0.01998240314424038,
0.006621686741709709,
-0.007115065585821867,
-0.025595271959900856,
-0.14406336843967438,
0.059341128915548325,
-0.05415617302060127,
0.02071075141429901,
-0.025673609226942062,
-0.03685189411044121,
-0.006687797140330076,
-0.024436241015791893,
0.04789716750383377,
0.012289738282561302,
-0.009464472532272339,
0.008874811232089996,
0.05439990013837814,
-0.017936402931809425,
0.015280311927199364,
0.019241321831941605,
0.019839845597743988,
-0.044581878930330276,
-0.00019122267258353531,
0.05710487812757492,
0.027124857529997826,
0.03286335989832878,
-0.03130904585123062,
0.030943499878048897,
0.044783297926187515,
-0.011470586061477661,
-0.02545638382434845,
-0.04851384460926056,
0.0374169684946537,
0.04311911389231682,
0.06186910718679428,
-0.007353961933404207,
0.04275231808423996,
-0.13535268604755402,
0.051532186567783356,
-0.0008787823026068509,
-0.013711938634514809,
-0.00963157881051302,
-0.05890839919447899,
-0.017307408154010773,
0.04525986686348915,
-0.05720233544707298,
0.014164116233587265,
-0.014212997630238533,
0.02781442180275917,
0.028117408975958824,
-0.030877774581313133,
-0.029513007029891014,
-0.04348914697766304,
-0.0023832593578845263,
-0.03131307661533356,
0.019721146672964096,
-0.03271373361349106,
-0.009591090492904186,
-0.02561146952211857,
0.03706233948469162,
-0.03820475935935974,
-0.02197130024433136,
0.06930754333734512,
0.0016996626509353518,
-0.02019146829843521,
0.02706538885831833,
-0.04186217114329338,
-0.011110583320260048,
-0.04420747980475426,
0.005779831670224667,
-0.0031908582895994186,
-0.01106533408164978,
0.0021898511331528425,
-0.061915501952171326,
0.36486107110977173,
0.02379745803773403,
-0.014334114268422127,
-0.10697902739048004,
-0.00672915019094944,
0.017174318432807922,
-0.03547753393650055,
-0.03425552695989609,
-0.07977989315986633,
-0.013417339883744717,
0.049696072936058044,
0.08452598750591278,
0.006001685280352831,
0.03887702897191048,
-0.05760378763079643,
-0.06360433995723724,
0.0018019960261881351,
0.03750760108232498,
-0.062107693403959274,
0.02396223321557045,
0.05404868349432945,
0.006083529442548752,
-0.021369706839323044,
0.028154900297522545,
-0.035313256084918976,
0.007669752463698387,
0.012345697730779648,
-0.028294293209910393,
0.07919534295797348,
0.03711969032883644,
-0.0059145488776266575,
0.059165988117456436,
-0.008629925549030304,
0.019830435514450073,
0.014452059753239155,
0.026252634823322296,
0.018986571580171585,
0.0500473789870739,
-0.00420206505805254,
0.06949815154075623,
0.02310183458030224,
-0.010984593071043491,
-0.0932149738073349,
0.002145858481526375,
-0.07226674258708954,
-0.09133562445640564,
0.12037825584411621,
0.03488070145249367,
-0.019075557589530945,
-0.0316641628742218,
0.025215981528162956,
0.024775445461273193,
0.04314354062080383,
-0.032355621457099915,
0.0198528915643692,
-0.02658434398472309,
0.05201643332839012,
-0.014923560433089733,
-0.014668277464807034,
-0.016024110838770866,
0.013716484420001507,
-0.08799697458744049,
0.022426869720220566,
-0.009626477025449276,
0.09660591930150986,
-0.021300561726093292,
-0.044487114995718,
0.016996432095766068,
0.03359347954392433,
0.013304057531058788,
0.07800822705030441,
0.006944891065359116,
-0.004945346619933844,
-0.05033111199736595,
0.02610575594007969,
-0.05167292430996895,
-0.03289158642292023,
0.0776744931936264,
-0.04914672672748566,
0.03185432031750679,
-0.007518535014241934,
0.05411246418952942,
0.06845714896917343,
-0.023407917469739914,
0.03571011871099472,
-0.0458809919655323,
0.014122110791504383,
0.00423531886190176,
0.03315233439207077,
0.009305421262979507,
-0.03714720904827118,
-0.01753867417573929,
0.011189483106136322,
-0.018542492762207985,
0.05962905287742615,
-0.08193671703338623,
-0.021736837923526764,
-0.04258403182029724,
0.021886376664042473,
-0.008782654069364071,
-0.011753150261938572,
0.08472330868244171,
0.041855357587337494,
0.002776537789031863,
0.041315943002700806,
0.07093818485736847,
0.08527335524559021,
-0.0008807986741885543,
-0.06306684762239456,
0.08339414745569229,
-0.042124323546886444,
-0.03398706391453743,
0.03836391121149063,
0.04599844664335251,
0.029053760692477226,
-0.05833232402801514,
0.05094488337635994,
0.01912022940814495,
-0.010215681977570057,
0.01786571554839611,
0.022066565230488777,
-0.04415921866893768,
0.011513312347233295,
0.0008795391186140478,
-0.3298252522945404,
-0.027384743094444275,
-0.013962769880890846,
-0.00925280712544918,
0.011228740215301514,
-0.0365125834941864,
0.028997719287872314,
-0.0005705010262317955,
-0.02805257774889469,
-0.012482876889407635,
0.019981255754828453,
0.09257366508245468,
-0.06907357275485992,
-0.06073439493775368,
0.034669484943151474,
0.02067163959145546,
0.06237587332725525,
0.03878159821033478,
0.028988704085350037,
0.0033881415147334337,
0.0414237454533577,
-0.019846105948090553,
-0.007420545909553766,
-0.03852342814207077,
0.04316362366080284,
-0.020995941013097763,
0.11153396964073181,
0.0098455511033535,
-0.012899099849164486,
0.012442725710570812,
-0.014208468608558178,
-0.0028977543115615845,
-0.029432695358991623,
-0.07914594560861588,
0.0371415838599205,
-0.007101075723767281,
0.05521168187260628,
-0.02026115544140339,
-0.040295377373695374,
-0.06468480825424194,
-0.08065488189458847,
0.00939533207565546,
0.02610481157898903,
-0.03118588775396347,
-0.07970193028450012,
-0.009445288218557835,
-0.02992747165262699,
-0.00861735362559557,
-0.046303555369377136,
0.00043012655805796385,
-0.02803691290318966,
0.03816216066479683,
-0.002619381994009018,
-0.021262304857373238,
-0.02586541138589382,
0.01061983685940504,
-0.09456813335418701,
0.007505123037844896,
-0.044008444994688034,
-0.03182459995150566,
0.04442368820309639,
0.027946926653385162,
0.0527968630194664,
-0.028957244008779526,
0.002336792415007949,
0.015204226598143578,
0.026626117527484894,
-0.01935010403394699,
0.04134231060743332,
0.017675014212727547,
-0.011271106079220772,
0.12707048654556274,
-0.11285753548145294,
0.04620211943984032,
0.054955389350652695,
-0.010323851369321346,
0.028928430750966072,
0.023272337391972542,
-0.05451557785272598,
0.022939642891287804,
-0.046720076352357864,
0.027105072513222694,
0.015591009519994259,
0.0007590267923660576,
-0.01756562851369381,
-0.05408209562301636,
0.02224883995950222,
-0.0498899482190609,
0.024156350642442703,
-0.009718735702335835,
0.008569128811359406,
0.035335198044776917,
-0.03679665923118591,
-0.08335030823945999,
-0.0022123807575553656,
0.06489405035972595,
-0.24540796875953674,
-0.0684068500995636,
0.07051894068717957,
-0.009469719603657722,
-0.028604252263903618,
-0.04008720442652702,
0.04220491275191307,
-0.0027736835181713104,
-0.0633881613612175,
-0.03442656248807907,
-0.04419008642435074,
0.03297748416662216,
0.053946636617183685,
0.017807217314839363,
-0.053527992218732834,
-0.02405509725213051,
0.08098474144935608,
0.011968836188316345,
-0.0007603206322528422,
-0.0001708500349195674,
-0.007417811546474695,
-0.056299060583114624,
0.1453978270292282,
0.03257717564702034,
0.0015509178629145026,
0.05832648277282715,
0.057947348803281784,
0.038300663232803345,
-0.05995640158653259,
0.03273699805140495,
0.020620234310626984,
-0.002061081351712346,
0.08712688088417053,
-0.014709554612636566,
-0.012756559997797012,
0.029576167464256287,
-0.06289421021938324,
-0.02131374180316925,
0.0036562748719006777,
0.0336427316069603,
-0.020358331501483917,
-0.00520337326452136,
-0.018868722021579742,
-0.0197067279368639,
0.0972212627530098,
-0.03203701972961426,
0.01400658581405878,
-0.04847543686628342,
-0.02281809039413929,
0.03705107048153877,
-0.025804677978157997,
-0.02423899807035923,
-0.003444890258833766,
0.006947638466954231,
0.01550942100584507,
-0.00959827285259962,
-0.027834894135594368,
-0.029471158981323242,
0.026401229202747345,
-0.06187841296195984,
-0.042514752596616745,
0.029395107179880142,
-0.0014921297552064061,
0.037216030061244965,
0.0695040374994278
] |
PMID:27204 | Performance studies with antihistamines. | 1 Effect of four antihistamines, chlorpheniramine (4 mg), clemastine (1 mg), promethazine (10 mg) and terfenadine (60 mg), on visuo-motor coordination and on subjective assessments of performance and well-being were compared with placebo in six healthy females from 0.5--7.0 h after morning ingestion of each drug. The study was double-blind, and the doses used were believed to be equally potent in their antihistaminic activity. 2 There was impaired performance 1.5 h (P less than 0.01) after chlorpheniramine, 3.0 h (P less than 0.05) and 5.0 h (P less than 0.01) after clemastine, and 3.0 h (P less than 0.01) and 5.0 h (P less than 0.001) after promethazine. It was not possible to establish effects on performance after ingestion of terfenadine. Subjective assessments of performance were not altered. 3 The subjects as a group reported improved alertness (P less than 0.05) and improved wakefulness (P less than 0.05) 0.5 h and 3.5 h respectively after ingestion of terfenadine, and were less energetic (P less than 0.05) 7.0 h after ingestion of chlorpheniramine. There were not other consistent changes in assessments of well-being. | Performance studies with antihistamines. 1 Effect of four antihistamines, chlorpheniramine (4 mg), clemastine (1 mg), promethazine (10 mg) and terfenadine (60 mg), on visuo-motor coordination and on subjective assessments of performance and well-being were compared with placebo in six healthy females from 0.5--7.0 h after morning ingestion of each drug. The study was double-blind, and the doses used were believed to be equally potent in their antihistaminic activity. 2 There was impaired performance 1.5 h (P less than 0.01) after chlorpheniramine, 3.0 h (P less than 0.05) and 5.0 h (P less than 0.01) after clemastine, and 3.0 h (P less than 0.01) and 5.0 h (P less than 0.001) after promethazine. It was not possible to establish effects on performance after ingestion of terfenadine. Subjective assessments of performance were not altered. 3 The subjects as a group reported improved alertness (P less than 0.05) and improved wakefulness (P less than 0.05) 0.5 h and 3.5 h respectively after ingestion of terfenadine, and were less energetic (P less than 0.05) 7.0 h after ingestion of chlorpheniramine. There were not other consistent changes in assessments of well-being. | [
-0.030907269567251205,
0.011518033221364021,
-0.016503890976309776,
0.05693833902478218,
-0.023102983832359314,
0.028980884701013565,
0.0646686851978302,
0.0502508208155632,
-0.046450283378362656,
-0.03883547708392143,
0.03244279697537422,
0.008106947876513004,
0.029639868065714836,
0.023636644706130028,
-0.0185876302421093,
-0.011115512810647488,
0.06739453226327896,
-0.022997332736849785,
-0.0669865757226944,
-0.05657130852341652,
-0.0863860696554184,
0.02316882088780403,
0.044666972011327744,
0.0028383315075188875,
-0.013996130786836147,
0.0024110444355756044,
-0.0075089712627232075,
-0.04159269109368324,
-0.04461761564016342,
-0.18081986904144287,
0.03262457251548767,
-0.04117894917726517,
-0.002015315927565098,
-0.0312805213034153,
-0.05367594584822655,
0.028550133109092712,
-0.05897793173789978,
0.0629928931593895,
0.026189684867858887,
-0.021967731416225433,
0.04033094272017479,
0.053342439234256744,
-0.02329898066818714,
0.0035784407518804073,
-0.01399129256606102,
0.03381836414337158,
-0.04123137891292572,
-0.026690252125263214,
0.08512922376394272,
-0.0038973211776465178,
-0.00447002612054348,
-0.009969686158001423,
0.038860175758600235,
0.056656818836927414,
0.03523716330528259,
0.018775198608636856,
-0.041918568313121796,
0.04389464855194092,
-0.011091857217252254,
0.03307639807462692,
-0.01677047647535801,
0.034428685903549194,
-0.1550983190536499,
0.03718985989689827,
0.014556958340108395,
0.003973154351115227,
-0.03446101397275925,
-0.03399205207824707,
-0.024526266381144524,
0.0737062394618988,
-0.03882064297795296,
0.024342697113752365,
0.0109684644266963,
0.022670509293675423,
0.04704585298895836,
-0.00410877913236618,
-0.042319104075431824,
-0.0522778257727623,
0.017595794051885605,
-0.005721397697925568,
0.014017222449183464,
0.011526033282279968,
-0.010144808329641819,
-0.05433552712202072,
0.042895831167697906,
-0.017376435920596123,
0.008188262581825256,
0.033127784729003906,
0.006155774928629398,
-0.033865828067064285,
-0.0031418788712471724,
-0.02001313492655754,
-0.039441049098968506,
-0.02880413457751274,
0.022783644497394562,
0.00902630016207695,
-0.021766142919659615,
-0.019641639664769173,
0.0010765476617962122,
0.3765314519405365,
0.0016853202832862735,
0.011852260679006577,
-0.08932317048311234,
-0.01810580864548683,
0.0344407819211483,
-0.01616981253027916,
0.020592961460351944,
-0.03606245666742325,
-0.0016593564068898559,
0.05885334685444832,
0.12318520247936249,
0.05206409841775894,
0.04210646450519562,
-0.010143048129975796,
-0.044749658554792404,
0.022377807646989822,
0.03860507532954216,
-0.029874134808778763,
0.0024628015235066414,
0.0386660173535347,
0.0011768318945541978,
-0.00916144996881485,
0.03458176925778389,
-0.028084315359592438,
0.005480686202645302,
0.021089550107717514,
-0.007965054363012314,
0.09533824026584625,
-0.0032024041283875704,
0.024324841797351837,
0.07295781373977661,
-0.03955148532986641,
0.00838632695376873,
0.029878530651330948,
0.03836384043097496,
0.00860833004117012,
0.05058680847287178,
0.000558743835426867,
0.030744431540369987,
0.013388607650995255,
-0.026790998876094818,
-0.10956043004989624,
0.027312112972140312,
-0.08926458656787872,
-0.06949101388454437,
0.1303824484348297,
0.059134356677532196,
-0.010878337547183037,
-0.02292787842452526,
0.039530858397483826,
0.006607582792639732,
0.03449999913573265,
-0.03838455304503441,
0.03775757923722267,
-0.023042969405651093,
0.01166618149727583,
-0.006445690058171749,
0.011470463126897812,
-0.017866352573037148,
0.005743278190493584,
-0.09145636111497879,
-0.0001307733473367989,
-0.030093137174844742,
0.08743434399366379,
-0.031516183167696,
0.022910993546247482,
-0.03502403199672699,
0.029944879934191704,
-0.033743053674697876,
0.05120893940329552,
0.023871690034866333,
0.008689843118190765,
-0.05365883558988571,
-0.012321198359131813,
-0.009606036357581615,
-0.036417119204998016,
0.08659045398235321,
-0.04026741907000542,
0.01646355353295803,
-0.008984009735286236,
0.03434770926833153,
0.05858810618519783,
-0.030579857528209686,
0.021575847640633583,
-0.010225539095699787,
0.026795169338583946,
0.0022042954806238413,
-0.00967031717300415,
0.05272701382637024,
0.01065146829932928,
-0.04358550161123276,
-0.016400178894400597,
-0.03486466780304909,
0.030151084065437317,
-0.0662476047873497,
-0.024054860696196556,
-0.08369161933660507,
0.050302036106586456,
0.00864268746227026,
-0.03936658054590225,
0.08009583503007889,
0.06322385370731354,
-0.007813068106770515,
0.06884943693876266,
0.04902879521250725,
0.06422106921672821,
-0.017218012362718582,
-0.07666551321744919,
0.07628611475229263,
-0.04721993952989578,
-0.03067813627421856,
0.014829970896244049,
0.07021060585975647,
0.010872382670640945,
-0.035465337336063385,
0.04183703660964966,
0.0181486327201128,
-0.001065943157300353,
0.019390752539038658,
0.023917345330119133,
-0.006981584709137678,
0.009832662530243397,
-0.045220647007226944,
-0.28509122133255005,
-0.009671710431575775,
0.008917168714106083,
-0.011578811332583427,
0.034026533365249634,
-0.012344613671302795,
-0.02430698089301586,
0.006645628716796637,
-0.010818098671734333,
-0.03966086730360985,
0.05922267213463783,
0.06997484713792801,
-0.0726112648844719,
-0.04004022851586342,
0.049200624227523804,
0.036758169531822205,
0.042196545749902725,
0.015119725838303566,
0.011204278096556664,
0.024779649451375008,
0.03787795826792717,
-0.04095715284347534,
-0.020862426608800888,
-0.028328360989689827,
0.023383038118481636,
-0.04232385754585266,
0.1204613745212555,
0.013235463760793209,
-0.0152266975492239,
-0.043453894555568695,
-0.03593425452709198,
0.04632853716611862,
-0.027986042201519012,
-0.11131685227155685,
0.014740883372724056,
0.0054266564548015594,
0.025099309161305428,
-0.031161870807409286,
-0.03897060453891754,
-0.05208360403776169,
-0.09074650704860687,
0.00006357082020258531,
0.0368192233145237,
-0.07510984688997269,
-0.06008508428931236,
-0.008560644462704659,
-0.05086387321352959,
-0.0007981447270140052,
-0.048755351454019547,
0.009703674353659153,
0.030852047726511955,
0.01961410790681839,
0.005254634190350771,
-0.010955176316201687,
-0.02317000739276409,
-0.003112007398158312,
-0.10597171634435654,
-0.004876383114606142,
-0.06958514451980591,
-0.004017602652311325,
0.014307655394077301,
0.015706343576312065,
0.04534340277314186,
-0.01979370228946209,
0.00344693660736084,
-0.003788443049415946,
0.026357661932706833,
-0.05085308849811554,
0.003637046553194523,
0.01591196469962597,
-0.017229072749614716,
0.10206792503595352,
-0.08346544206142426,
-0.0016726590692996979,
0.05586075782775879,
-0.017993194982409477,
-0.028416436165571213,
-0.014413369819521904,
-0.07559385895729065,
0.04113885760307312,
-0.018675174564123154,
0.07952368259429932,
0.0110127879306674,
0.00863739661872387,
-0.024588925763964653,
-0.05339885130524635,
0.025051556527614594,
-0.058469709008932114,
0.031486015766859055,
0.017761940136551857,
0.03410845994949341,
0.03692193329334259,
-0.013043793849647045,
-0.02047419361770153,
0.03306220471858978,
0.08171816170215607,
-0.25689592957496643,
-0.006791071500629187,
0.08247924596071243,
-0.02443935163319111,
0.03473174199461937,
-0.03515031561255455,
0.04792121425271034,
-0.04034348204731941,
-0.02664181776344776,
-0.022866329178214073,
-0.013369311578571796,
0.06407902389764786,
0.04178542643785477,
0.001998156076297164,
-0.03099273145198822,
-0.05424140393733978,
0.06016583368182182,
0.0003142838831990957,
-0.0030280412174761295,
0.003996555227786303,
0.0027802346739917994,
-0.03987612947821617,
0.15323416888713837,
0.055186741054058075,
0.009612033143639565,
0.0424187108874321,
0.04288487136363983,
0.04396819695830345,
-0.03284434601664543,
0.02520493045449257,
-0.018424879759550095,
0.008413552306592464,
0.08005067706108093,
-0.02018173411488533,
0.00002264824797748588,
-0.034265562891960144,
-0.050638552755117416,
-0.028232242912054062,
0.000007436641226377105,
0.04534474387764931,
0.004595885518938303,
0.02102423645555973,
-0.010614839382469654,
-0.016526132822036743,
0.10086548328399658,
-0.04800623282790184,
-0.020164182409644127,
-0.07075141370296478,
-0.02666991949081421,
-0.002763271564617753,
-0.043796006590127945,
-0.04402193799614906,
0.037234727293252945,
-0.024272028356790543,
-0.018096283078193665,
0.004237356595695019,
-0.014232928864657879,
-0.009283922612667084,
0.02708078920841217,
-0.06241264566779137,
-0.031238188967108727,
0.005922624375671148,
-0.0031001348979771137,
0.03696422651410103,
0.07091323286294937
] |
PMID:27205 | The acute effects of the administration of rimiterol aerosol in asthmatic children. | 1 Ten asthmatic children received on two separate occasions rimiterol (500 microgram) and orciprenaline (1.5 mg) as aerosols in a double-blind, crossover design trial. Respiratory parameters (FEV1, FVC and PEFR) and pulse rate were recorded for 60 min after each administration. 2 Rimiterol produced effective bronchodilation in children with negligible cardiac stimulation. It differed from orciprenaline in that the peak bronchodilator effect was not maintained during the 60 min observation period. 3 Rimiterol is a selective, short-acting, sympathomimetic bronchodilator in children. | The acute effects of the administration of rimiterol aerosol in asthmatic children. 1 Ten asthmatic children received on two separate occasions rimiterol (500 microgram) and orciprenaline (1.5 mg) as aerosols in a double-blind, crossover design trial. Respiratory parameters (FEV1, FVC and PEFR) and pulse rate were recorded for 60 min after each administration. 2 Rimiterol produced effective bronchodilation in children with negligible cardiac stimulation. It differed from orciprenaline in that the peak bronchodilator effect was not maintained during the 60 min observation period. 3 Rimiterol is a selective, short-acting, sympathomimetic bronchodilator in children. | [
-0.025262081995606422,
-0.06293962150812149,
0.02028784528374672,
0.015242806635797024,
-0.00991267804056406,
0.01953837089240551,
0.05638573318719864,
0.02472364343702793,
-0.05370429530739784,
-0.039983201771974564,
-0.012143774889409542,
-0.03191943094134331,
-0.015389897860586643,
-0.049296166747808456,
0.019532423466444016,
0.038127996027469635,
0.1185775175690651,
-0.049093082547187805,
-0.07997002452611923,
-0.003850409993901849,
-0.05640454217791557,
0.015388020314276218,
0.0569218285381794,
-0.009609686210751534,
0.03137996792793274,
0.015498287975788116,
-0.011131038889288902,
-0.03408888727426529,
-0.026184406131505966,
-0.14759418368339539,
0.08574478328227997,
-0.034694794565439224,
0.015617262572050095,
-0.02581973373889923,
-0.05268631875514984,
0.05172206088900566,
-0.026341622695326805,
0.057357266545295715,
-0.006231141742318869,
0.004555892199277878,
0.010101454332470894,
0.010334819555282593,
0.016188576817512512,
0.012855728156864643,
-0.05255432426929474,
0.0215061753988266,
-0.018554847687482834,
-0.05029817298054695,
0.06961669027805328,
-0.011890496127307415,
0.05823502317070961,
-0.032344549894332886,
0.023489488288760185,
0.02887531742453575,
-0.029476040974259377,
-0.0009198904153890908,
-0.03147819638252258,
0.015099787153303623,
0.012606474570930004,
-0.011320273391902447,
-0.02442462183535099,
0.08707249164581299,
-0.1401611715555191,
0.02241847664117813,
-0.00124593754298985,
0.047500431537628174,
-0.04411943256855011,
-0.0017754361033439636,
0.017295703291893005,
0.07572740316390991,
-0.004168523941189051,
0.018292009830474854,
-0.021233832463622093,
0.07874307781457901,
-0.013488108292222023,
0.0365191325545311,
-0.04078926518559456,
-0.03254750743508339,
0.010485186241567135,
-0.02328347973525524,
0.0425022728741169,
0.03374361991882324,
-0.047882385551929474,
0.017260491847991943,
0.0536828376352787,
-0.01715974509716034,
0.06091828644275665,
-0.02137141302227974,
0.005585032980889082,
-0.053488947451114655,
0.0042025321163237095,
-0.006536430679261684,
-0.036393988877534866,
0.023758558556437492,
-0.04905959591269493,
-0.012354600243270397,
-0.016461798921227455,
-0.037557464092969894,
-0.02291666343808174,
0.3733638525009155,
0.024191586300730705,
0.02477392740547657,
-0.07140295207500458,
-0.014658499509096146,
0.024879157543182373,
-0.026155779138207436,
0.0011843941174447536,
-0.039735645055770874,
-0.021557927131652832,
0.019092688336968422,
0.06618612259626389,
-0.05492531508207321,
0.06629037857055664,
-0.018169362097978592,
-0.0379406213760376,
-0.006252430845052004,
0.03475464507937431,
0.008870761841535568,
-0.0028220154345035553,
0.05685897171497345,
-0.01672988198697567,
0.014921877533197403,
0.030359771102666855,
-0.004201563075184822,
0.038937732577323914,
0.018546022474765778,
0.02891215682029724,
0.0947977676987648,
0.0034373754169791937,
0.060139793902635574,
0.11408336460590363,
-0.002553967060521245,
-0.005533229559659958,
-0.003446269780397415,
0.03740697354078293,
-0.030506998300552368,
0.03830994665622711,
-0.0006609847769141197,
0.02034824900329113,
-0.03352545574307442,
-0.012826094403862953,
-0.07423891872167587,
-0.023053085431456566,
-0.08950096368789673,
-0.08218236267566681,
0.13967081904411316,
0.05321212112903595,
0.02769957110285759,
-0.0068090204149484634,
0.007573452312499285,
-0.029320405796170235,
0.029075462371110916,
-0.02784373238682747,
0.05525793880224228,
-0.022858193144202232,
0.04115857183933258,
-0.024245643988251686,
0.08348572254180908,
0.04660199582576752,
-0.01663968898355961,
-0.08336122334003448,
-0.029242688789963722,
0.02018580213189125,
0.04867029935121536,
-0.0489175021648407,
-0.03429845720529556,
-0.014154981821775436,
0.016192246228456497,
-0.029374167323112488,
0.033538300544023514,
0.05744333937764168,
-0.006803018040955067,
-0.008811639621853828,
-0.02785441093146801,
-0.038283638656139374,
-0.021217908710241318,
0.016044870018959045,
-0.006938281003385782,
0.08711312711238861,
-0.03200094774365425,
0.035339873284101486,
0.027876855805516243,
0.05210970342159271,
0.014203582890331745,
0.010671386495232582,
-0.0225856751203537,
-0.041704051196575165,
-0.017472365871071815,
0.06610690802335739,
0.00386101845651865,
-0.03943260759115219,
-0.029223959892988205,
-0.10634161531925201,
0.008338830433785915,
-0.05095897614955902,
-0.045840051025152206,
-0.040719904005527496,
-0.005838307086378336,
0.00843719020485878,
0.035639286041259766,
0.07466591894626617,
0.03606702387332916,
0.048818670213222504,
0.06232383847236633,
-0.001777343568392098,
0.06255052238702774,
-0.017672600224614143,
-0.033910419791936874,
0.03852345421910286,
-0.0908447653055191,
0.0031986439134925604,
0.01588757149875164,
-0.010183270089328289,
-0.018392162397503853,
-0.07209113985300064,
0.10074963420629501,
-0.053688667714595795,
0.009287865832448006,
0.042718857526779175,
-0.01619006134569645,
0.01725378818809986,
-0.03248650208115578,
0.04014519602060318,
-0.3190873861312866,
0.008041358552873135,
0.008856702595949173,
-0.01595880463719368,
0.1083235964179039,
-0.019980045035481453,
0.014693894423544407,
-0.008933160454034805,
-0.006776381283998489,
-0.0657840296626091,
0.03958631679415703,
0.00020257342839613557,
-0.033198099583387375,
0.0259653739631176,
-0.011694865301251411,
0.07969722896814346,
0.03339435160160065,
0.012941122986376286,
0.026676364243030548,
-0.006530396640300751,
-0.0027983752079308033,
0.0026282204780727625,
-0.07991447299718857,
-0.025228338316082954,
0.016404403373599052,
-0.0621233694255352,
0.08300022780895233,
-0.03657229617238045,
-0.0620860792696476,
0.03526798263192177,
0.011005147360265255,
0.003547268221154809,
0.00013515808677766472,
-0.0034749277401715517,
0.0014906589640304446,
-0.015026847831904888,
-0.005610525608062744,
0.027331918478012085,
-0.018425675109028816,
-0.0136724216863513,
-0.03098268434405327,
0.06936889886856079,
0.03769518435001373,
-0.07956430315971375,
-0.06844525784254074,
-0.01265227422118187,
-0.025886785238981247,
0.049778543412685394,
-0.08447887748479843,
-0.038434263318777084,
-0.006414689589291811,
0.014454953372478485,
-0.026323992758989334,
-0.007580439560115337,
0.04650301858782768,
-0.07480235397815704,
-0.060462586581707,
0.03454066812992096,
-0.04998230189085007,
-0.02591405063867569,
0.03295336663722992,
0.015677768737077713,
0.06225161999464035,
-0.03309115394949913,
-0.06107360124588013,
-0.01708603836596012,
0.04984535649418831,
-0.03424565866589546,
0.00505664199590683,
0.052493587136268616,
0.0325639545917511,
0.1170714870095253,
-0.10062610357999802,
0.0036885379813611507,
0.045983269810676575,
-0.0017216354608535767,
-0.020300088450312614,
-0.03812411427497864,
-0.01991421729326248,
0.012102165259420872,
-0.04846245422959328,
-0.009068910963833332,
0.011998853646218777,
0.04486003518104553,
-0.024426976218819618,
-0.05455417186021805,
0.002140153432264924,
-0.012370958924293518,
0.008993741124868393,
-0.013386434875428677,
-0.010419430211186409,
0.012766335159540176,
0.005520020145922899,
0.02217782661318779,
0.03249676898121834,
0.06648413091897964,
-0.22965534031391144,
-0.003359366673976183,
0.029268885031342506,
0.00542053859680891,
-0.019663356244564056,
-0.04767449200153351,
0.012029954232275486,
0.008799666538834572,
0.0087662972509861,
-0.020713429898023605,
-0.019136831164360046,
0.004314736928790808,
0.07791560888290405,
0.04408764839172363,
-0.030541876330971718,
0.026406142860651016,
0.04250507801771164,
-0.02673271857202053,
0.009960999712347984,
-0.021465927362442017,
0.020889950916171074,
-0.044073235243558884,
0.10881856828927994,
-0.007685468066483736,
-0.06782019883394241,
0.023389829322695732,
-0.013421799056231976,
0.03190471604466438,
-0.03332510590553284,
0.0224145594984293,
-0.010010071098804474,
-0.04876158758997917,
0.11522023379802704,
-0.034887269139289856,
-0.021199000999331474,
0.0038716618437319994,
-0.09397169202566147,
0.015492723323404789,
-0.03254450112581253,
0.058889783918857574,
-0.044587522745132446,
0.020735101774334908,
0.021743614226579666,
-0.01265083160251379,
0.05487453192472458,
-0.0626913383603096,
0.006786528043448925,
-0.046446964144706726,
-0.0012965357163920999,
-0.033650219440460205,
0.0007691010250709951,
-0.018315043300390244,
0.05222512409090996,
0.09531568735837936,
0.037724193185567856,
0.0277934018522501,
-0.030214417725801468,
-0.0333469919860363,
-0.00105272326618433,
0.027225760743021965,
-0.046814460307359695,
0.034211818128824234,
-0.01861656829714775,
0.08907674252986908,
0.0327092669904232
] |
PMID:27206 | Periarteritis nodosa and thrombotic thrombocytopenic purpura with serous retinal detachment in siblings. | Periarteritis nodosa and thrombotic thrombocytopenic purpura in siblings is reported. In both patients a localised serous retinal detachment and lesions of the retinal pigment epithelium had developed owing to choroidal vascular obstruction. These cases support the suggested possible relationship between the two conditions. | Periarteritis nodosa and thrombotic thrombocytopenic purpura with serous retinal detachment in siblings. Periarteritis nodosa and thrombotic thrombocytopenic purpura in siblings is reported. In both patients a localised serous retinal detachment and lesions of the retinal pigment epithelium had developed owing to choroidal vascular obstruction. These cases support the suggested possible relationship between the two conditions. | [
-0.0022524907253682613,
-0.010564856231212616,
-0.03992612287402153,
-0.013614281080663204,
0.03213449567556381,
-0.017818905413150787,
0.08267679065465927,
0.030542200431227684,
-0.03582553565502167,
-0.011449160985648632,
0.012657037936151028,
-0.10694253444671631,
0.03535793721675873,
0.06290078908205032,
-0.03831031918525696,
-0.010465829633176327,
-0.03708627447485924,
0.030205700546503067,
-0.02520833909511566,
0.004103551618754864,
-0.05986284837126732,
0.0022658277302980423,
0.014730525203049183,
-0.01741519384086132,
0.002707400359213352,
0.04424498230218887,
-0.024672510102391243,
-0.004782662261277437,
-0.025876671075820923,
-0.14361658692359924,
-0.006768753752112389,
-0.04181266203522682,
-0.05179852619767189,
0.007174397353082895,
0.03353186696767807,
0.01006853673607111,
-0.05523745343089104,
0.04874122142791748,
-0.004099277313798666,
0.00046579408808611333,
-0.00023937353398650885,
-0.04785122349858284,
-0.02380436845123768,
-0.010593938641250134,
0.03848767280578613,
0.0010076864855363965,
-0.0004469698469620198,
0.023686334490776062,
0.11044761538505554,
0.022507578134536743,
-0.017995642498135567,
-0.01314893364906311,
0.07726548612117767,
0.04917675256729126,
-0.05744226649403572,
0.018409861251711845,
0.00020202355517540127,
-0.02728470228612423,
0.03585060313344002,
0.03221189230680466,
0.006672844290733337,
0.016976751387119293,
-0.11408907175064087,
0.05447731912136078,
0.031434137374162674,
-0.0031858286820352077,
0.012235365808010101,
-0.016855547204613686,
-0.019186485558748245,
0.009846372529864311,
-0.09412434697151184,
0.02129610814154148,
-0.011542420834302902,
0.05377041921019554,
-0.03329364210367203,
-0.03991064429283142,
0.01127557922154665,
0.011523157358169556,
0.02335991896688938,
0.03999553248286247,
-0.038975607603788376,
0.0777292549610138,
-0.036876920610666275,
-0.04711780697107315,
-0.013062921352684498,
0.0586387999355793,
0.017091521993279457,
-0.0936853364109993,
-0.025104323402047157,
0.010847102850675583,
-0.022547375410795212,
-0.039837319403886795,
-0.03923654183745384,
-0.020653214305639267,
-0.08030448853969574,
-0.0029490587767213583,
0.02733822539448738,
-0.046216025948524475,
-0.02291332371532917,
0.3968597948551178,
-0.02718263678252697,
-0.02103782258927822,
0.013518866151571274,
-0.0027824079152196646,
0.022067103534936905,
0.004233678802847862,
0.03865555301308632,
-0.045608047395944595,
-0.038040366023778915,
0.035061683505773544,
0.025383582338690758,
-0.0071820104494690895,
0.05425305292010307,
-0.0007181116379797459,
-0.02749587967991829,
-0.029398465529084206,
0.00865689106285572,
0.02383274957537651,
-0.03428341820836067,
0.05264019966125488,
0.009868715889751911,
0.027463451027870178,
-0.0025649170856922865,
-0.0014747751411050558,
-0.003968842327594757,
0.023831594735383987,
0.06893007457256317,
0.09544804692268372,
0.05083474889397621,
-0.04923398792743683,
0.09034344553947449,
0.014207329601049423,
-0.022324299439787865,
-0.00894632376730442,
0.020374702289700508,
0.015227964147925377,
0.02592560462653637,
-0.07216542959213257,
0.03975798562169075,
-0.010319402441382408,
0.012711815536022186,
-0.06424416601657867,
-0.02715487778186798,
-0.0422959104180336,
-0.051072001457214355,
0.06141389533877373,
-0.058515675365924835,
0.04995828494429588,
-0.05848403647542,
0.0006906528724357486,
0.00886855460703373,
0.05819273740053177,
-0.02284163236618042,
0.03481991961598396,
0.0013188933953642845,
0.002615113975480199,
-0.06319092214107513,
0.047070905566215515,
0.041643496602773666,
0.040245234966278076,
0.05297501012682915,
-0.010995207354426384,
-0.0511968731880188,
0.043839965015649796,
0.06276401877403259,
-0.056886881589889526,
-0.009301476180553436,
-0.07619141787290573,
-0.02851155400276184,
0.044202063232660294,
-0.08798369020223618,
0.050527311861515045,
-0.021781055256724358,
-0.005828839261084795,
-0.0283109899610281,
0.06720205396413803,
-0.01760745421051979,
0.006794714834541082,
-0.026499222964048386,
0.05748346447944641,
0.052476633340120316,
-0.007464786525815725,
-0.01279760804027319,
0.08357241749763489,
0.016632748767733574,
-0.010995828546583652,
-0.03561048209667206,
-0.017744267359375954,
0.07080540806055069,
0.016203586012125015,
-0.05256931483745575,
-0.019970502704381943,
-0.06667182594537735,
0.0038451687432825565,
0.044513482600450516,
0.02057586796581745,
-0.06830775737762451,
-0.04114146530628204,
-0.0449000708758831,
-0.002684920560568571,
0.06320439279079437,
-0.04065430909395218,
-0.04290061071515083,
-0.005347740836441517,
0.024253901094198227,
0.005659920163452625,
-0.026221131905913353,
-0.00591932563111186,
-0.05911252647638321,
-0.007609305903315544,
0.00014425501285586506,
0.006769758649170399,
-0.0119772432371974,
0.032802317291498184,
-0.05048440769314766,
0.01417490653693676,
-0.029810044914484024,
0.00013715788372792304,
0.0294752586632967,
0.03175938129425049,
0.033111318945884705,
-0.02366659976541996,
0.023083467036485672,
-0.27123069763183594,
-0.03268783539533615,
-0.003456501057371497,
-0.06308428198099136,
-0.047938164323568344,
-0.06794733554124832,
-0.043225985020399094,
-0.028016790747642517,
0.0024898836854845285,
0.019275622442364693,
0.046052485704422,
0.08820222318172455,
0.0038783946074545383,
-0.025879578664898872,
0.004515967797487974,
0.07592581957578659,
0.022001421079039574,
-0.019441286101937294,
0.022719426080584526,
-0.05026460438966751,
-0.0004115623305551708,
-0.0024220929481089115,
-0.059075623750686646,
0.017109744250774384,
0.06584864109754562,
0.0633416399359703,
0.1483186036348343,
0.00868574995547533,
-0.030907437205314636,
-0.02279602736234665,
0.05436220020055771,
0.04134276509284973,
-0.0069572534412145615,
-0.039686646312475204,
-0.013828696683049202,
-0.008590810932219028,
-0.044338028877973557,
-0.03907965496182442,
-0.012536264024674892,
0.012925942428410053,
-0.030638497322797775,
-0.04368019849061966,
-0.05629103258252144,
0.014990746043622494,
-0.029234599322080612,
0.036034490913152695,
0.03645238280296326,
0.09742875397205353,
0.08219274133443832,
0.0898907333612442,
-0.01104422565549612,
-0.09102508425712585,
-0.013388327322900295,
0.01013165432959795,
0.0511079877614975,
0.020141396671533585,
-0.03562547639012337,
-0.011924867518246174,
-0.052271995693445206,
-0.03971873223781586,
0.06266061216592789,
0.03863147273659706,
0.06549496948719025,
-0.07878383249044418,
0.017474086955189705,
0.016520589590072632,
0.050053469836711884,
0.003008341183885932,
0.12831053137779236,
0.0713476911187172,
-0.05672517791390419,
0.07935522496700287,
0.004778587259352207,
-0.020223567262291908,
0.024335702881217003,
0.038772549480199814,
0.052835509181022644,
-0.03537112474441528,
-0.04184380918741226,
-0.010200864635407925,
0.044464852660894394,
-0.09511030465364456,
-0.005430296529084444,
0.03677640110254288,
0.010529451072216034,
-0.01590527407824993,
-0.004668455105274916,
-0.05278240889310837,
-0.06839098036289215,
0.005076776258647442,
0.024779437109827995,
0.01639692485332489,
-0.07254459708929062,
0.009506701491773129,
0.002310978714376688,
0.002893679076805711,
-0.28144559264183044,
0.023358842357993126,
0.023777058348059654,
-0.03996055945754051,
0.0015399339608848095,
0.014259959571063519,
0.08439505845308304,
-0.03505237028002739,
-0.0016918701585382223,
0.019351795315742493,
0.0492706336081028,
0.03295508399605751,
0.0031020098831504583,
0.03555484861135483,
-0.04479152709245682,
0.03813677653670311,
0.037810176610946655,
0.03337765857577324,
-0.00581022584810853,
-0.023612257093191147,
-0.005320258438587189,
-0.039838265627622604,
0.15606556832790375,
-0.03170841932296753,
-0.010121363215148449,
-0.007759429980069399,
-0.03808795288205147,
0.04851766675710678,
-0.04184241592884064,
0.0131723927333951,
-0.03294845297932625,
0.03236130252480507,
0.034410398453474045,
0.007031119428575039,
0.034088704735040665,
0.034206610172986984,
-0.010489057749509811,
0.00742765236645937,
-0.001174022676423192,
-0.003034476190805435,
-0.020030051469802856,
-0.013194587081670761,
-0.08039873093366623,
-0.02118951827287674,
0.026474257931113243,
-0.027026453986763954,
-0.05249753221869469,
0.025709839537739754,
0.03471527248620987,
0.02857726812362671,
0.014616349712014198,
-0.012975280173122883,
0.0029761060141026974,
0.00812247022986412,
0.07313349097967148,
-0.05709453299641609,
-0.011339222081005573,
0.0004534048493951559,
-0.002165586920455098,
-0.061057448387145996,
0.03470950201153755,
0.021747278049588203,
-0.0336565263569355,
0.07555790990591049,
-0.01866757497191429
] |
PMID:27207 | Gastric secretion in suckling pigs and early-weaned pigs given a dry cow's-mild formula ad lib. | 1. Twelve gastric-cannulated litter-mate pigs were used to study secretion and proteolytic activity in the stomach of suckling and early-weaned pigs in relation to age and food intake. 2. Results demonstrate that from the first observation at day 8, piglets were able to secrete acid. pH and acid concentration did not change during the first 4 weeks of life. 3. Proteolytic activity was low during the first 2--3 weeks of life and rapidly increased thereafter. 4. Two phenomena differentiated suckling pigs from pigs given dry cow's milk: (1) a low buffering capacity the gastric contents, beginning 1 hr after feeding the dry cow's-milk formula, results in a low total acid concentration in the weaned pigs and (2) the increase in proteolytic activity in relation to the age is much more pronounced in the artificially-reared pigs. 5. These two phenomena are discussed and related to the formation of a hard casein clot in the stomach of the cow's-milk-fed pigs, which has a long retention time and stimulates gastrin release. | Gastric secretion in suckling pigs and early-weaned pigs given a dry cow's-mild formula ad lib. 1. Twelve gastric-cannulated litter-mate pigs were used to study secretion and proteolytic activity in the stomach of suckling and early-weaned pigs in relation to age and food intake. 2. Results demonstrate that from the first observation at day 8, piglets were able to secrete acid. pH and acid concentration did not change during the first 4 weeks of life. 3. Proteolytic activity was low during the first 2--3 weeks of life and rapidly increased thereafter. 4. Two phenomena differentiated suckling pigs from pigs given dry cow's milk: (1) a low buffering capacity the gastric contents, beginning 1 hr after feeding the dry cow's-milk formula, results in a low total acid concentration in the weaned pigs and (2) the increase in proteolytic activity in relation to the age is much more pronounced in the artificially-reared pigs. 5. These two phenomena are discussed and related to the formation of a hard casein clot in the stomach of the cow's-milk-fed pigs, which has a long retention time and stimulates gastrin release. | [
0.014780701138079166,
-0.0013522893423214555,
-0.0036134780384600163,
0.020042480900883675,
0.015558804385364056,
-0.005179800558835268,
0.044234227389097214,
0.00887652300298214,
-0.004811156541109085,
-0.04068891331553459,
-0.03547210618853569,
-0.10473986715078354,
-0.003172752447426319,
0.03711505979299545,
0.02925916761159897,
-0.009342076256871223,
-0.024162715300917625,
-0.029044372960925102,
-0.08240672945976257,
-0.04966743290424347,
-0.011199203319847584,
0.029201233759522438,
0.007235608994960785,
-0.03435336798429489,
0.03256690129637718,
0.0227887574583292,
0.007597208023071289,
0.03347344323992729,
-0.0492340624332428,
-0.19356143474578857,
0.04142763465642929,
-0.06668578088283539,
-0.015053424052894115,
-0.012759849429130554,
-0.010676071047782898,
0.011409983970224857,
0.03297487273812294,
0.010281439870595932,
-0.02168411947786808,
0.08190552890300751,
0.12558455765247345,
-0.000322992738801986,
-0.02429869771003723,
0.0027142763137817383,
-0.0050932434387505054,
0.05365622043609619,
-0.04533415660262108,
0.02467753179371357,
0.061718620359897614,
0.008641013875603676,
0.017435263842344284,
0.02831425704061985,
0.009619193151593208,
0.027318431064486504,
0.031140534207224846,
-0.042621124535799026,
0.04769543930888176,
0.02796313539147377,
0.006809284910559654,
-0.012664448469877243,
-0.014539450407028198,
0.02375412918627262,
-0.1375470757484436,
0.033398646861314774,
0.07373753190040588,
0.02242589369416237,
-0.05233258754014969,
-0.05352330207824707,
0.04332466423511505,
0.05346985533833504,
-0.0591130256652832,
0.039406780153512955,
0.031248746439814568,
0.003011261811479926,
-0.0026710035745054483,
0.0038693395908921957,
0.047756608575582504,
0.03851882740855217,
0.028673730790615082,
-0.023408807814121246,
-0.04659762233495712,
0.05681236460804939,
0.034652162343263626,
-0.0011452871840447187,
-0.04034131020307541,
0.01124755758792162,
-0.02005820721387863,
-0.0006870438228361309,
0.017563199624419212,
-0.005882374010980129,
-0.04205809161067009,
-0.05413475260138512,
-0.042774904519319534,
0.004360639024525881,
-0.035066332668066025,
-0.006173551082611084,
0.03263256326317787,
-0.06738820672035217,
-0.021814825013279915,
0.3843104839324951,
-0.05522586405277252,
0.008727984502911568,
-0.005354601889848709,
-0.026280026882886887,
0.007779726758599281,
-0.000716018897946924,
-0.007244485896080732,
0.036497849971055984,
-0.009959838353097439,
0.04191714525222778,
0.03953086957335472,
0.07978425174951553,
0.025870472192764282,
-0.03637422248721123,
0.0027216747403144836,
0.014553454704582691,
0.020054928958415985,
0.025373538956046104,
0.01897960714995861,
0.07419579476118088,
-0.010974337346851826,
0.024522120133042336,
-0.0022788827773183584,
-0.0143998758867383,
0.04337470978498459,
-0.00039086007745936513,
0.006209053564816713,
0.05819560959935188,
-0.020562827587127686,
-0.012787904590368271,
0.01533885020762682,
0.002312783384695649,
-0.010935347527265549,
0.021220244467258453,
-0.014207364991307259,
-0.08329539000988007,
0.015106156468391418,
0.05187127739191055,
0.049564655870199203,
-0.06740336120128632,
-0.051362812519073486,
-0.04795515537261963,
0.022290771827101707,
-0.1276065856218338,
-0.01804582215845585,
0.0768795907497406,
0.05040635168552399,
0.035235412418842316,
-0.0018376654479652643,
0.008259756490588188,
0.028527485206723213,
0.03128395974636078,
0.05359282344579697,
0.03405517712235451,
0.014186285436153412,
0.006034088786691427,
-0.03207826241850853,
0.0047319987788796425,
-0.005784051027148962,
-0.03328428044915199,
-0.0636226087808609,
-0.042313553392887115,
0.0169783066958189,
-0.02718687430024147,
0.08880943059921265,
-0.02463846281170845,
0.027837703004479408,
-0.056838151067495346,
0.03844377025961876,
-0.03927601873874664,
-0.0005418254295364022,
0.034832924604415894,
-0.08194179832935333,
-0.03694041445851326,
-0.026604723185300827,
-0.007243701722472906,
0.012536250986158848,
-0.004182856529951096,
0.028797293081879616,
0.019303971901535988,
-0.04419270530343056,
-0.010003381408751011,
0.002085978165268898,
0.005993704777210951,
0.014004794880747795,
0.0029442310333251953,
-0.0784572884440422,
0.034239575266838074,
-0.004618138074874878,
0.09141600877046585,
-0.028548570349812508,
-0.02567480318248272,
-0.12344547361135483,
-0.03555934503674507,
-0.046759191900491714,
-0.029024068266153336,
-0.005747364368289709,
-0.019014928489923477,
-0.04216744378209114,
0.022772284224629402,
0.029876362532377243,
-0.039775505661964417,
-0.008821282535791397,
0.02828778512775898,
0.006025101989507675,
0.03280649334192276,
0.020924894139170647,
-0.039179619401693344,
0.06220109388232231,
-0.045155711472034454,
0.0014590793289244175,
0.04674713686108589,
-0.07560400664806366,
0.012789185158908367,
-0.06690268218517303,
0.09796006977558136,
-0.021852221339941025,
-0.02793913148343563,
0.010917854495346546,
-0.015430714935064316,
0.03257351741194725,
-0.0304748322814703,
0.007199233863502741,
-0.3008032739162445,
-0.03840533271431923,
0.06935548037290573,
-0.05873612314462662,
0.13709130883216858,
-0.044035837054252625,
-0.012239319272339344,
-0.030782684683799744,
0.024141527712345123,
-0.03984057158231735,
0.06693333387374878,
0.03993982821702957,
-0.05348242074251175,
0.04844636842608452,
0.0638885349035263,
0.059351664036512375,
0.040424033999443054,
-0.03762790933251381,
0.04865916073322296,
0.02878279611468315,
0.022693756967782974,
-0.03162035718560219,
0.02522711083292961,
0.015472392551600933,
0.03565187007188797,
0.027492765337228775,
0.11636638641357422,
-0.006204501260071993,
0.016410749405622482,
-0.01171957515180111,
-0.0189164187759161,
0.03195192664861679,
-0.008425218053162098,
-0.04463869333267212,
-0.043438639491796494,
-0.007080041337758303,
-0.015271982178092003,
-0.0023730292450636625,
-0.001148777431808412,
-0.011231906712055206,
-0.04892375320196152,
-0.00897214189171791,
-0.00843552965670824,
-0.08051741123199463,
-0.048937879502773285,
-0.007904238067567348,
0.035294726490974426,
0.03441208228468895,
-0.008292612619698048,
-0.017379412427544594,
0.0336652435362339,
0.04374438151717186,
0.005823247600346804,
0.017062373459339142,
-0.04980197176337242,
-0.033148620277643204,
0.021838486194610596,
0.003521630074828863,
-0.08232792466878891,
0.018905002623796463,
-0.015244312584400177,
0.0015005678869783878,
-0.006126313470304012,
-0.017384564504027367,
-0.02342497929930687,
-0.015418916009366512,
0.0035527264699339867,
-0.03843020275235176,
-0.01416639145463705,
0.030195465311408043,
0.014946279115974903,
0.10873741656541824,
-0.038245830684900284,
-0.024584678933024406,
0.03769506514072418,
-0.014618619345128536,
-0.01976454071700573,
-0.06877638399600983,
-0.061478305608034134,
-0.014463664963841438,
0.01639876514673233,
-0.09282850474119186,
0.06892871111631393,
-0.0058451928198337555,
0.025595571845769882,
0.05978305637836456,
0.03672058507800102,
-0.04014137014746666,
0.02036569081246853,
-0.008008701726794243,
0.013750557787716389,
-0.03504585474729538,
-0.08665456622838974,
-0.013180136680603027,
-0.013993468135595322,
0.03041151724755764,
-0.27632251381874084,
0.03402191773056984,
0.01408128160983324,
-0.01238388940691948,
-0.00853276439011097,
0.016253195703029633,
0.04255203157663345,
-0.025585828348994255,
-0.009915659204125404,
0.014865895733237267,
0.018738407641649246,
-0.004498266614973545,
0.07809246331453323,
0.017669789493083954,
-0.004853899125009775,
0.027039039880037308,
-0.020095601677894592,
-0.042275503277778625,
-0.002416100585833192,
-0.024078920483589172,
0.03374790400266647,
-0.0671522319316864,
0.14550654590129852,
0.027441857382655144,
0.010365338996052742,
0.03501245006918907,
-0.0069475844502449036,
0.013361713849008083,
0.01925298571586609,
-0.007921356707811356,
0.05338147655129433,
0.032534509897232056,
0.06004907935857773,
0.038896989077329636,
0.00332224415615201,
0.03455159813165665,
-0.0009881164878606796,
-0.006625352893024683,
-0.048637524247169495,
0.0341586172580719,
-0.003208010923117399,
0.011321703903377056,
-0.05119117721915245,
-0.031653061509132385,
0.04510674625635147,
-0.12327422201633453,
0.02498949132859707,
-0.04934374988079071,
0.028071343898773193,
0.012803999707102776,
-0.03216477856040001,
-0.08934340626001358,
0.03413963317871094,
0.05455486476421356,
0.03368786722421646,
-0.00567639572545886,
0.034143198281526566,
-0.026027781888842583,
-0.04834900051355362,
0.049055203795433044,
0.050595737993717194,
-0.019354157149791718,
0.015541092492640018,
0.08850808441638947,
-0.01892266422510147
] |
PMID:27210 | Studies on the microsomal mixed function oxidase system: redox properties of detergent-solubilized NADPH-cytochrome P-450 reductase. | Hepatic microsomal NADPH-cytochrome P-450 reductase was solubilized from rabbit liver microsomes in the presence of detergents and purified to homogeneity by column chromatography. The purified reductase had a molecular weight of 78 000 and contained 1 mol each of FAD and FMN per mol of enzyme. On reduction with NADPH in the presence of molecular oxygen, an 02-stable semiquinone containing one flavin free radical per two flavins was formed, in agreement with previous work on purified trypsin-solubilized reductase. The reduction of oxidized enzyme by NADPH, and autoxidation of NADPH-reduced enzyme by air, proceeded by both one-electron equivalent and two-electron equivalent mechanisms. The reductase reduced cytochrome P-450 (from phenobarbital-treated rabbits) and cytochrome P-448 (from 3-methylcholanthrene-treated rabbits). The rate of reduction of cytochrome P-450 increased in the presence of a substrate, benzphetamine, but that of cytochrome P-448 did not. | Studies on the microsomal mixed function oxidase system: redox properties of detergent-solubilized NADPH-cytochrome P-450 reductase. Hepatic microsomal NADPH-cytochrome P-450 reductase was solubilized from rabbit liver microsomes in the presence of detergents and purified to homogeneity by column chromatography. The purified reductase had a molecular weight of 78 000 and contained 1 mol each of FAD and FMN per mol of enzyme. On reduction with NADPH in the presence of molecular oxygen, an 02-stable semiquinone containing one flavin free radical per two flavins was formed, in agreement with previous work on purified trypsin-solubilized reductase. The reduction of oxidized enzyme by NADPH, and autoxidation of NADPH-reduced enzyme by air, proceeded by both one-electron equivalent and two-electron equivalent mechanisms. The reductase reduced cytochrome P-450 (from phenobarbital-treated rabbits) and cytochrome P-448 (from 3-methylcholanthrene-treated rabbits). The rate of reduction of cytochrome P-450 increased in the presence of a substrate, benzphetamine, but that of cytochrome P-448 did not. | [
-0.07442571222782135,
0.029194554314017296,
-0.06120153144001961,
0.07654847949743271,
0.023026999086141586,
-0.014001977629959583,
0.006917003076523542,
0.017390863969922066,
-0.03834347799420357,
-0.03266780823469162,
-0.04476383328437805,
-0.04993056505918503,
-0.001552143949083984,
-0.0014612161321565509,
-0.028343865647912025,
-0.03515700250864029,
0.02254198119044304,
-0.01698981411755085,
-0.057560112327337265,
0.014694321900606155,
-0.05487250164151192,
0.0068880291655659676,
0.02719212882220745,
0.018086565658450127,
0.04119080677628517,
0.033331166952848434,
0.02423844113945961,
-0.04180629551410675,
-0.05059501528739929,
-0.18202881515026093,
0.0494670607149601,
0.005749044474214315,
0.025637654587626457,
-0.032918933779001236,
0.04518699273467064,
-0.0005829925066791475,
-0.025054268538951874,
-0.01702122576534748,
-0.0026678896974772215,
0.040626347064971924,
0.08951623737812042,
0.008250252343714237,
0.039865825325250626,
0.04423025995492935,
0.01771136187016964,
0.019556522369384766,
-0.05652998015284538,
0.029311422258615494,
0.09893938899040222,
-0.04462366923689842,
0.02781600132584572,
0.007697646971791983,
-0.013108870014548302,
0.003503846237435937,
0.05665592849254608,
-0.022882794961333275,
0.041539885103702545,
0.06626704335212708,
-0.010421322658658028,
0.02605816163122654,
-0.03747563436627388,
0.04768560081720352,
-0.1299581229686737,
0.060427743941545486,
0.05478113889694214,
-0.02473589777946472,
-0.005779204424470663,
-0.03479966148734093,
0.0649564191699028,
0.040438566356897354,
-0.07607127726078033,
0.034348513931035995,
-0.04568445682525635,
-0.014247060753405094,
0.010367775335907936,
-0.042727258056402206,
-0.0010686840396374464,
-0.05343405529856682,
0.03911086544394493,
0.020472818985581398,
0.04256877675652504,
0.021091829985380173,
-0.007049332372844219,
-0.0036495146341621876,
0.02548855170607567,
0.057913921773433685,
-0.0005820101359859109,
-0.01683012954890728,
0.015386003069579601,
-0.010652616620063782,
-0.015177622437477112,
-0.052613068372011185,
0.005407165270298719,
-0.042109109461307526,
-0.03872855752706528,
0.04991350322961807,
0.05481334030628204,
0.0281034205108881,
0.0029705737251788378,
0.3585616946220398,
0.009865987114608288,
0.03271568566560745,
-0.05421466752886772,
-0.037439845502376556,
-0.01887587271630764,
-0.049416907131671906,
0.003889811458066106,
0.007760567124933004,
0.022526469081640244,
0.04371044784784317,
0.029552407562732697,
-0.03666500374674797,
0.024906083941459656,
-0.05241130664944649,
-0.051631100475788116,
0.026986055076122284,
-0.03144870325922966,
-0.025656424462795258,
0.018709665164351463,
0.0593104362487793,
0.017450030893087387,
-0.02610325627028942,
0.03438301384449005,
0.017489323392510414,
0.029988808557391167,
0.008742456324398518,
0.02230369858443737,
0.11268743127584457,
0.06376802921295166,
0.0866665467619896,
-0.02202715538442135,
0.022395730018615723,
0.03287480026483536,
-0.031289879232645035,
-0.05687706917524338,
0.01593446359038353,
0.029875971376895905,
-0.04218219220638275,
0.013723611831665039,
-0.024835119023919106,
-0.06703635305166245,
-0.0876540020108223,
-0.054548993706703186,
-0.051771171391010284,
-0.10594023764133453,
0.0684809684753418,
0.026461398229002953,
0.017625659704208374,
0.011300959624350071,
-0.0010216257069259882,
0.027600446715950966,
0.054242413491010666,
-0.0528402179479599,
0.03075147233903408,
-0.01944691501557827,
0.061567652970552444,
-0.007874754257500172,
0.0008735823212191463,
0.03809821233153343,
0.0026453265454620123,
-0.11884516477584839,
-0.04046228155493736,
-0.005757753737270832,
0.09611861407756805,
0.0013007480883970857,
-0.034009698778390884,
-0.016638679429888725,
0.04022444412112236,
0.04041234776377678,
-0.033298492431640625,
0.02591952122747898,
-0.06880432367324829,
-0.025573207065463066,
-0.03541653975844383,
-0.04080527648329735,
0.03180674463510513,
0.06561081856489182,
0.056382838636636734,
0.07885029911994934,
0.004940565209835768,
0.01877664588391781,
0.009656046517193317,
-0.04046507552266121,
0.054335810244083405,
0.05045018345117569,
0.008906803093850613,
-0.010404864326119423,
0.035327959805727005,
0.0137964291498065,
0.05139726772904396,
-0.06138332188129425,
0.02291126549243927,
-0.0519639290869236,
-0.019620241597294807,
-0.036003243178129196,
-0.02833622507750988,
-0.01851155236363411,
0.009084969758987427,
0.0011475514620542526,
-0.006015519145876169,
0.1398533284664154,
0.025583097711205482,
0.031584788113832474,
0.026428833603858948,
-0.045376069843769073,
0.04511412978172302,
0.03606599569320679,
0.07672341167926788,
0.035935793071985245,
0.03475916385650635,
-0.04463142156600952,
0.012966032139956951,
-0.024530373513698578,
0.01919618807733059,
-0.04604645445942879,
0.014303741045296192,
-0.012159902602434158,
-0.05619974434375763,
0.06199335679411888,
0.04038457199931145,
0.014044566079974174,
-0.05985509231686592,
-0.06311162561178207,
-0.30213335156440735,
-0.04061651602387428,
0.01090039312839508,
-0.017821399495005608,
0.021779460832476616,
-0.015961669385433197,
0.024267245084047318,
-0.013589996844530106,
0.012294805608689785,
0.005137358792126179,
0.04352450370788574,
0.019935090094804764,
-0.06308943033218384,
-0.019200479611754417,
-0.014337916858494282,
0.030378203839063644,
0.013344325125217438,
-0.06252182275056839,
-0.025144249200820923,
0.018030770123004913,
0.027455748990178108,
-0.011467771604657173,
0.04503358155488968,
0.017755987122654915,
0.09448469430208206,
0.0328020304441452,
0.10802772641181946,
-0.03822219744324684,
-0.03570770472288132,
0.007964549586176872,
0.036836471408605576,
0.01862497068941593,
-0.054322611540555954,
-0.09217328578233719,
-0.05005551874637604,
-0.03766581043601036,
-0.04285493493080139,
-0.0828932374715805,
0.042032450437545776,
0.01775079034268856,
-0.025886157527565956,
0.03223535791039467,
-0.003401922294870019,
-0.08802143484354019,
-0.06233347952365875,
-0.028293034061789513,
-0.02644505724310875,
-0.04381242021918297,
0.0029782182537019253,
0.014825952239334583,
-0.007731913588941097,
0.02348809875547886,
0.02690020017325878,
-0.040298983454704285,
0.05000835284590721,
-0.01079972367733717,
-0.04307745397090912,
0.008283641189336777,
-0.06762672960758209,
-0.014632412232458591,
-0.016366681084036827,
-0.026777368038892746,
-0.04878455027937889,
-0.003243879182264209,
0.01624482125043869,
0.00034384854370728135,
-0.03417795151472092,
-0.010462594218552113,
0.04036818817257881,
0.004281069152057171,
0.022994665428996086,
0.10631480813026428,
-0.00011223209730815142,
0.0045887199230492115,
0.024948518723249435,
0.024812716990709305,
-0.01540460530668497,
0.043002478778362274,
-0.0829060971736908,
0.05540831387042999,
-0.027150413021445274,
-0.008681066334247589,
0.06648658961057663,
0.0470685251057148,
-0.036850761622190475,
-0.022470418363809586,
0.0031754656229168177,
-0.03637845069169998,
-0.0019272990757599473,
0.012038429267704487,
0.022570166736841202,
-0.022967670112848282,
-0.006443631369620562,
-0.04507461562752724,
0.02664773538708687,
0.049831945449113846,
-0.2508460581302643,
-0.02021235227584839,
0.005238763056695461,
-0.01621556095778942,
-0.02693391963839531,
0.02609720639884472,
0.06900329142808914,
-0.029581237584352493,
0.03155248612165451,
0.03348596394062042,
0.02565700188279152,
0.032225415110588074,
0.09590340405702591,
0.05345214903354645,
0.009319500997662544,
0.022617263719439507,
0.0075791445560753345,
-0.002571506192907691,
-0.021861286833882332,
-0.010446658357977867,
0.025525499135255814,
-0.037902552634477615,
0.08396731317043304,
-0.045553285628557205,
-0.0027130215894430876,
0.05462165176868439,
0.005319327116012573,
0.017734073102474213,
-0.06382196396589279,
0.03667492792010307,
-0.017810435965657234,
0.021262627094984055,
0.08648335933685303,
-0.06572031229734421,
0.017897874116897583,
0.009940015152096748,
-0.047372061759233475,
0.006701801437884569,
0.0035996013320982456,
0.01823047362267971,
-0.03247405216097832,
-0.0422549732029438,
-0.10089945048093796,
-0.07850075513124466,
0.05919982120394707,
-0.07673493772745132,
-0.08485499769449234,
-0.04651897773146629,
0.016730889678001404,
0.009253302589058876,
-0.06819208711385727,
-0.03708845376968384,
0.03306448832154274,
0.03092624805867672,
0.000875521800480783,
-0.0097510302439332,
-0.03539750725030899,
0.034453898668289185,
0.01290995255112648,
-0.019366728141903877,
0.002485073870047927,
0.03876311704516411,
-0.05002572014927864,
0.11371166259050369,
0.004406449384987354
] |
PMID:27211 | Reactions of reduced nicotinamide adenine dinucleotide in acid: studies by reversed-phase high-pressure liquid chromatography. | Reversed-phase high-pressure liquid chromatography was used to isolate acid breakdown products of reduced nicotinamide adenine dinucleotide (NADH) and products produced when NADH breakdown is catalyzed by glyceraldehyde-3-phosphate dehydrogenase (G-3-PD). Chromatographic and UV spectral data on these and related products support a mechanism for NADH acid degradation involving hydroxy addition at the nicotinamide C-6 followed by cyclization of the ring and the adjacent ribose moiety. G-3-PD is shown to catalyze a reaction in which two products are formed which are also intermediates in the acid degradation of NADH (alpha- and beta-6-hydroxynicotinamide products). Formation of the major acid products fits a three-step, first-order mechanism curve, making it possible to calculate the rate constants k2 and k3 as well as the previously determined k1. | Reactions of reduced nicotinamide adenine dinucleotide in acid: studies by reversed-phase high-pressure liquid chromatography. Reversed-phase high-pressure liquid chromatography was used to isolate acid breakdown products of reduced nicotinamide adenine dinucleotide (NADH) and products produced when NADH breakdown is catalyzed by glyceraldehyde-3-phosphate dehydrogenase (G-3-PD). Chromatographic and UV spectral data on these and related products support a mechanism for NADH acid degradation involving hydroxy addition at the nicotinamide C-6 followed by cyclization of the ring and the adjacent ribose moiety. G-3-PD is shown to catalyze a reaction in which two products are formed which are also intermediates in the acid degradation of NADH (alpha- and beta-6-hydroxynicotinamide products). Formation of the major acid products fits a three-step, first-order mechanism curve, making it possible to calculate the rate constants k2 and k3 as well as the previously determined k1. | [
-0.057033970952034,
0.02343308925628662,
-0.01010334212332964,
0.014854754321277142,
0.054660603404045105,
-0.004186272621154785,
0.010726936161518097,
-0.0037416834384202957,
-0.06279391795396805,
-0.005010376684367657,
0.033397506922483444,
-0.045362479984760284,
0.05508148670196533,
0.016909876838326454,
-0.042521439492702484,
-0.06783126294612885,
0.01682344265282154,
0.0063505591824650764,
-0.024425813928246498,
-0.02470504306256771,
-0.02577524445950985,
-0.014518813230097294,
0.02014346420764923,
0.02519143745303154,
0.043966956436634064,
0.05652837082743645,
0.019543278962373734,
-0.008636394515633583,
-0.08702952414751053,
-0.21577560901641846,
0.037360530346632004,
0.02965187467634678,
-0.006931365001946688,
-0.0974922925233841,
0.020317278802394867,
0.0525570809841156,
-0.016566811129450798,
0.0334356464445591,
-0.012327756732702255,
0.018941808491945267,
0.04456149414181709,
-0.016840403899550438,
-0.03299015760421753,
0.039044566452503204,
0.004445354919880629,
-0.01201818697154522,
-0.05964905023574829,
-0.020128361880779266,
0.07625798881053925,
-0.036587636917829514,
-0.03640034422278404,
0.0380118153989315,
0.008412259630858898,
-0.04410938546061516,
0.012988320551812649,
-0.01100220251828432,
0.07744215428829193,
0.050837401300668716,
-0.030097270384430885,
-0.013180495239794254,
0.0205976702272892,
0.01620529592037201,
-0.16954092681407928,
0.031435634940862656,
0.08532417565584183,
0.01703617535531521,
0.03143707662820816,
-0.037755824625492096,
0.02786317467689514,
0.024932200089097023,
-0.12042701989412308,
-0.004174238536506891,
0.02086537517607212,
-0.015872498974204063,
0.009243828244507313,
-0.020906243473291397,
0.015930090099573135,
-0.017299270257353783,
0.027502218261361122,
-0.036046091467142105,
0.03215514123439789,
0.017736468464136124,
0.0010397287551313639,
0.01072408352047205,
-0.01699376478791237,
-0.0076342676766216755,
0.019613273441791534,
-0.019928690046072006,
0.0062518855556845665,
0.013000857084989548,
-0.01891777478158474,
-0.06897607445716858,
-0.0042907423339784145,
-0.0004930006107315421,
-0.09606023132801056,
0.028018182143568993,
-0.003358637448400259,
0.006862632930278778,
0.018546871840953827,
0.41877344250679016,
0.016245299950242043,
0.004445185419172049,
-0.0476112961769104,
-0.08378540724515915,
-0.04133332893252373,
-0.0920131653547287,
-0.03805091232061386,
0.024475742131471634,
0.05975896492600441,
0.032195109874010086,
0.0581636056303978,
0.005369479767978191,
0.027910130098462105,
-0.10416948050260544,
0.0265127532184124,
0.007600358687341213,
0.016021069139242172,
-0.043086979538202286,
-0.026515381410717964,
0.027492646127939224,
0.03874741494655609,
0.013434251770377159,
-0.00011284193169558421,
0.003636222565546632,
0.020792338997125626,
-0.012696818448603153,
-0.023995768278837204,
0.09006186574697495,
0.06479834020137787,
-0.012362427078187466,
0.020651625469326973,
-0.007638504263013601,
-0.062090154737234116,
0.00243317405693233,
-0.018222318962216377,
0.03891454637050629,
0.05221210792660713,
0.013033528812229633,
0.01602216809988022,
-0.012481193989515305,
-0.0241811815649271,
-0.010708284564316273,
-0.044006966054439545,
-0.019404055550694466,
0.007246603257954121,
0.04771348088979721,
-0.03185407444834709,
0.06365755945444107,
-0.023787589743733406,
0.028314698487520218,
0.05009787157177925,
0.07392095029354095,
-0.00434815464541316,
0.01592303067445755,
0.0033673124853521585,
0.0235829409211874,
-0.026907315477728844,
-0.05255765840411186,
-0.01590072736144066,
0.03153062239289284,
-0.04982323199510574,
-0.026230789721012115,
0.03247871622443199,
0.0713476911187172,
-0.01623079925775528,
0.0001753552642185241,
0.0050139883533120155,
0.00021150211978238076,
0.030484849587082863,
0.01028520055115223,
-0.00893518328666687,
0.004600939340889454,
-0.004378197249025106,
-0.03147480636835098,
-0.04813824221491814,
-0.0034921003971248865,
0.054605044424533844,
0.04513019695878029,
0.07127659767866135,
-0.00012754127965308726,
-0.00008188439096556976,
-0.008114581927657127,
-0.042828381061553955,
0.028027912601828575,
0.058220844715833664,
-0.007512444630265236,
-0.03567121922969818,
0.09658490866422653,
-0.015949353575706482,
0.06212034821510315,
-0.04894745349884033,
-0.007481872104108334,
-0.103507861495018,
0.04656822979450226,
-0.017919227480888367,
-0.015186982229351997,
-0.025294870138168335,
-0.005277219228446484,
-0.006979597732424736,
-0.0019027907401323318,
0.10775846987962723,
0.008785421960055828,
-0.003920047543942928,
0.05103428289294243,
-0.0012431982904672623,
0.0031486109364777803,
-0.004643049091100693,
0.03693209961056709,
0.006554967723786831,
0.000410718988860026,
0.021591082215309143,
0.014601899310946465,
-0.010053408332169056,
0.03746309131383896,
-0.08245519548654556,
-0.05515746772289276,
-0.03786632418632507,
0.02701135352253914,
0.004682272206991911,
0.01830684393644333,
0.020220570266246796,
-0.06064971536397934,
-0.0487038679420948,
-0.29867327213287354,
-0.02260085567831993,
0.06775397807359695,
-0.043289750814437866,
0.06071304902434349,
-0.021016357466578484,
0.026932330802083015,
-0.023938845843076706,
0.0051302071660757065,
-0.04119792953133583,
0.07312500476837158,
0.055099230259656906,
-0.045462466776371,
-0.05188286304473877,
-0.037930961698293686,
0.047277338802814484,
0.00504420418292284,
-0.06209561973810196,
0.011229547671973705,
0.02745530940592289,
0.0275130458176136,
0.010042096488177776,
0.012239670380949974,
0.003636577632278204,
0.030887961387634277,
-0.012775890529155731,
0.06985477358102798,
0.004658358171582222,
0.019302017986774445,
0.017123229801654816,
0.021463461220264435,
0.03844475373625755,
-0.0845293253660202,
-0.04959037899971008,
-0.05335794389247894,
-0.001045434852130711,
-0.04565122351050377,
-0.051252346485853195,
0.004835663828998804,
-0.019788920879364014,
0.007717627566307783,
-0.01868763007223606,
0.007803252898156643,
-0.06197836995124817,
-0.0026105265133082867,
-0.014299334026873112,
-0.026165472343564034,
0.038100168108940125,
0.03485814854502678,
0.02325344644486904,
0.08406676352024078,
0.02992910146713257,
0.015690365806221962,
-0.0560867078602314,
0.01894812099635601,
0.0020256424322724342,
-0.06914380937814713,
0.03809191286563873,
-0.08828563988208771,
-0.02576592192053795,
0.003987899515777826,
0.00632854038849473,
-0.04778732731938362,
0.029153907671570778,
0.019800301641225815,
0.0005983307491987944,
0.013574915006756783,
-0.003087021876126528,
0.008497444912791252,
-0.0009427640470676124,
0.034294117242097855,
0.10928460210561752,
-0.06285879015922546,
-0.004561574198305607,
0.021212656050920486,
0.02429373376071453,
-0.0362933985888958,
-0.050669051706790924,
-0.05611984804272652,
-0.00270057306624949,
0.008439667522907257,
-0.03825504705309868,
0.09579532593488693,
-0.037671349942684174,
-0.010663595981895924,
0.01618562452495098,
0.032079342752695084,
-0.026550907641649246,
0.03178063780069351,
-0.0015816101804375648,
0.03598799929022789,
-0.000016784306353656575,
0.014541269280016422,
-0.04576295241713524,
0.061243489384651184,
0.02959143929183483,
-0.26085004210472107,
-0.018348563462495804,
0.037055518478155136,
0.0019881564658135176,
-0.0035575523506850004,
0.07858157902956009,
0.017440151423215866,
-0.011475126259028912,
-0.029845697805285454,
0.05034750699996948,
-0.01513102650642395,
0.046923376619815826,
-0.018971623852849007,
0.0333043709397316,
0.03274707868695259,
-0.030485624447464943,
0.01787990890443325,
-0.03501056507229805,
0.02242041938006878,
-0.01845049485564232,
0.03899245709180832,
-0.03577660396695137,
0.12674833834171295,
-0.0002737057802733034,
-0.04395978897809982,
0.010946928523480892,
-0.003172490280121565,
0.03194763883948326,
-0.05247298255562782,
0.0006633229786530137,
-0.06275999546051025,
0.022188900038599968,
0.06990513205528259,
-0.005406044889241457,
0.040195535868406296,
0.039424676448106766,
-0.04662400856614113,
0.012281837873160839,
-0.0014117227401584387,
0.025000330060720444,
-0.022088591009378433,
-0.008983556181192398,
-0.025822965428233147,
-0.014745617285370827,
0.08521895855665207,
-0.0771653801202774,
-0.09377719461917877,
-0.06782808899879456,
0.031621020287275314,
0.029589561745524406,
-0.004150256048887968,
0.0045952280052006245,
0.04603797197341919,
0.03792669251561165,
-0.06543925404548645,
-0.027027815580368042,
-0.01291096955537796,
-0.028108840808272362,
0.0260675810277462,
0.0070562115870416164,
0.03151542320847511,
0.006509541533887386,
-0.02560535818338394,
0.08019484579563141,
0.02544223517179489
] |
PMID:27212 | Affinity chromatography of H+-translocating adenosine triphosphatase isolated by chloroform extraction of Rhodospirillum rubrum chromatophores. Modification of binding affinity by divalent cations and activating anions. | 1. ATPase isolated from Rhodospirillum rubrum by chloroform extraction and purified by gel filtration or affinity chromatography shows three bands (alpha, beta and gamma) upon electrophoresis in sodium dodecyl sulphate. 2. Ca2+-ATPase activity of the preparation is inhibited by aurovertin and efrapeptin but not by oligomycin. Activity may be inhibited by treatment with 4-chloro-7-nitrobenzofurazan and subsequently restored by dithiothreitol. 3. The enzyme fails to reconstitute photophosphorylation in chromatophores depleted of ATPase by sonic irradiation. 4. Most of the active protein from the crude chloroform extract binds to an affinity chromatography column bearing an immobilised ADP analogue but not to a column bearing immobilised pyrophosphate. 5. In the absence of divalent cations, a component with a very high specific activity for Ca2+-ATPase is eluted from the column by 1.6 mM ATP. This protein migrates asa single band on 5% polyacrylamide gel electrophoresis and only possesses three subunits. At 12 mM ATP an inactive protein is eluted which does not run on acid or alkali polyacrylamide gels and shows a complex subunit structure. 6. ATPase preparations prepared by acetone extraction or by sonic irradiation of chromatophores may also be purified 10-fold by affinity chromatography. 7. The inclusion of 5 mM MgCl2 or CaCl2 during affinity chromatography of chloroform ATPase increases the capacity of the column for the enzyme and demands a higher eluting concentration of ATP. 8. When the enzyme is more than 90% inhibited by efrapeptin or 4-chloro-7-nitrobenzofurazan, the binding characteristics of the enzyme are not affected. 9. 10 mM Na2SO3, which greatly stimulates the Ca2+- and Mg2+-dependent ATPase activity of the enzyme and increases Ki (ADP) for Ca2+-ATPase from 50 to 850 micron, prevents binding to the affinity column. Binding may be restored by the addition of divalent cations. 10. Na2SO3 increases the rate of ATP hydrolysis, ATP-driven H+ translocation and ATP-driven transhydrogenase in chromatophores. 11. It is proposed that anions such as sulphite convert the chromatophore ATPase into a form which is a more efficient energy transducer. | Affinity chromatography of H+-translocating adenosine triphosphatase isolated by chloroform extraction of Rhodospirillum rubrum chromatophores. Modification of binding affinity by divalent cations and activating anions. 1. ATPase isolated from Rhodospirillum rubrum by chloroform extraction and purified by gel filtration or affinity chromatography shows three bands (alpha, beta and gamma) upon electrophoresis in sodium dodecyl sulphate. 2. Ca2+-ATPase activity of the preparation is inhibited by aurovertin and efrapeptin but not by oligomycin. Activity may be inhibited by treatment with 4-chloro-7-nitrobenzofurazan and subsequently restored by dithiothreitol. 3. The enzyme fails to reconstitute photophosphorylation in chromatophores depleted of ATPase by sonic irradiation. 4. Most of the active protein from the crude chloroform extract binds to an affinity chromatography column bearing an immobilised ADP analogue but not to a column bearing immobilised pyrophosphate. 5. In the absence of divalent cations, a component with a very high specific activity for Ca2+-ATPase is eluted from the column by 1.6 mM ATP. This protein migrates asa single band on 5% polyacrylamide gel electrophoresis and only possesses three subunits. At 12 mM ATP an inactive protein is eluted which does not run on acid or alkali polyacrylamide gels and shows a complex subunit structure. 6. ATPase preparations prepared by acetone extraction or by sonic irradiation of chromatophores may also be purified 10-fold by affinity chromatography. 7. The inclusion of 5 mM MgCl2 or CaCl2 during affinity chromatography of chloroform ATPase increases the capacity of the column for the enzyme and demands a higher eluting concentration of ATP. 8. When the enzyme is more than 90% inhibited by efrapeptin or 4-chloro-7-nitrobenzofurazan, the binding characteristics of the enzyme are not affected. 9. 10 mM Na2SO3, which greatly stimulates the Ca2+- and Mg2+-dependent ATPase activity of the enzyme and increases Ki (ADP) for Ca2+-ATPase from 50 to 850 micron, prevents binding to the affinity column. Binding may be restored by the addition of divalent cations. 10. Na2SO3 increases the rate of ATP hydrolysis, ATP-driven H+ translocation and ATP-driven transhydrogenase in chromatophores. 11. It is proposed that anions such as sulphite convert the chromatophore ATPase into a form which is a more efficient energy transducer. | [
-0.09680914878845215,
-0.027523552998900414,
-0.02053874544799328,
0.03271370753645897,
0.02138226293027401,
-0.05971849709749222,
0.05092329531908035,
0.046189773827791214,
-0.07122243940830231,
-0.02165396325290203,
0.07308743894100189,
-0.05560605600476265,
0.0013359473086893559,
0.023450899869203568,
-0.018330415710806847,
-0.05027204751968384,
0.020282447338104248,
0.03795313090085983,
0.02057708613574505,
0.00688980333507061,
-0.025070620700716972,
-0.007243872154504061,
-0.009980197995901108,
-0.006393139250576496,
0.039137739688158035,
0.09784136712551117,
0.007067441940307617,
-0.031174499541521072,
-0.054230015724897385,
-0.19764821231365204,
-0.012187059037387371,
-0.032568760216236115,
0.005712977144867182,
-0.047963760793209076,
0.030770212411880493,
0.05167386308312416,
-0.020944932475686073,
-0.03133535757660866,
-0.030916856601834297,
0.05702412873506546,
0.0006198271876201034,
0.04485543444752693,
-0.02049998566508293,
0.04413822665810585,
-0.010284935124218464,
0.016150563955307007,
-0.006146842148154974,
0.02370571345090866,
0.09633879363536835,
0.02287820726633072,
-0.028028711676597595,
0.016804935410618782,
0.03919833526015282,
0.021270478144288063,
-0.021902522072196007,
0.03237997367978096,
0.02447870373725891,
0.05277375504374504,
-0.005888747982680798,
-0.027806075289845467,
0.038662780076265335,
0.059129610657691956,
-0.1450425386428833,
0.02302694134414196,
0.04045477509498596,
-0.038681257516145706,
0.01906653866171837,
-0.008369553834199905,
0.07121505588293076,
0.07930588722229004,
-0.02483479492366314,
0.019482379779219627,
-0.005688552279025316,
0.02432812564074993,
-0.02580960839986801,
-0.030664680525660515,
-0.02190597541630268,
-0.038102950900793076,
0.04344422370195389,
0.0025590199511498213,
-0.04818570986390114,
0.01285246480256319,
-0.013312370516359806,
-0.0010804560733959079,
-0.02622232586145401,
0.05846719443798065,
0.020083559677004814,
-0.051850516349077225,
0.01424732431769371,
0.05229330062866211,
0.005065014120191336,
-0.07674883306026459,
0.0061654276214540005,
-0.025635529309511185,
-0.08215959370136261,
0.006022926885634661,
0.010250098071992397,
0.0329204685986042,
0.04745253920555115,
0.3378651440143585,
0.011345683597028255,
0.023131830617785454,
-0.052365195006132126,
0.015966182574629784,
0.010114891454577446,
-0.05725674703717232,
-0.04390610009431839,
0.008215244859457016,
-0.005583364516496658,
0.042374398559331894,
0.04511181637644768,
-0.024135436862707138,
0.020218444988131523,
-0.03754343092441559,
0.008079384453594685,
0.020872153341770172,
-0.013231395743787289,
0.04338319972157478,
-0.06039978936314583,
0.056164633482694626,
-0.06611286848783493,
-0.05006753280758858,
0.06429439783096313,
-0.0276806503534317,
0.08725466579198837,
0.028784455731511116,
-0.038723934441804886,
0.0808168426156044,
0.09001856297254562,
-0.017393313348293304,
0.04027578607201576,
-0.008624597452580929,
-0.053408436477184296,
0.03518010303378105,
0.028206070885062218,
-0.01398665551096201,
0.05870898813009262,
-0.047453224658966064,
0.024050896987318993,
-0.009128500707447529,
-0.01849159225821495,
-0.03036544658243656,
-0.06726141273975372,
-0.0342341847717762,
-0.04695280268788338,
0.09219591319561005,
-0.009330859407782555,
0.02703056111931801,
-0.005514516495168209,
-0.04066498205065727,
-0.010743053629994392,
0.08191756904125214,
-0.010597463697195053,
0.0378199964761734,
-0.07128297537565231,
0.055986445397138596,
0.00903154443949461,
0.03503170982003212,
-0.011731411330401897,
0.011291710659861565,
-0.11931515485048294,
-0.030818641185760498,
0.05622924119234085,
0.05246425420045853,
-0.03438611701130867,
-0.0015116978902369738,
-0.02620108611881733,
0.00500877620652318,
-0.04964273422956467,
0.01588275469839573,
0.010100655257701874,
0.017609253525733948,
0.02911866456270218,
0.004646469838917255,
-0.05327008664608002,
0.026569191366434097,
0.037439607083797455,
0.012506833299994469,
0.03373049944639206,
-0.03632005304098129,
-0.04660147428512573,
0.014791584573686123,
-0.025681795552372932,
-0.014693974517285824,
0.01345076784491539,
-0.0036856532096862793,
-0.03986464813351631,
0.05944400653243065,
-0.02370867133140564,
0.07406698167324066,
-0.014635736122727394,
-0.0007458373438566923,
-0.06766682863235474,
0.055512167513370514,
-0.04597155377268791,
-0.045118071138858795,
-0.011571163311600685,
0.03607568144798279,
0.014227377250790596,
-0.03404277190566063,
0.08095034211874008,
0.028085095807909966,
-0.02144400030374527,
0.027373358607292175,
-0.01831255666911602,
0.05307070165872574,
-0.040295906364917755,
0.048095591366291046,
0.0054384940303862095,
0.018933234736323357,
-0.016633175313472748,
0.03369017690420151,
-0.07425252348184586,
-0.006592745892703533,
-0.08693525195121765,
0.043668098747730255,
0.005196748301386833,
-0.024583397433161736,
0.05088622123003006,
-0.012882210314273834,
-0.0011183465830981731,
-0.03967541456222534,
-0.05320480465888977,
-0.3254695534706116,
-0.02291652001440525,
-0.0033750187139958143,
-0.007585264276713133,
0.044439490884542465,
-0.029451990500092506,
0.08354900032281876,
0.02599659189581871,
-0.007502193097025156,
-0.09462052583694458,
-0.003212074050679803,
0.059686269611120224,
-0.05007149651646614,
-0.04811964929103851,
0.014076564460992813,
0.004370785318315029,
0.0023023325484246016,
0.001326005905866623,
0.024663515388965607,
-0.029443329200148582,
0.039314981549978256,
0.01805591769516468,
0.01775042526423931,
0.07860839366912842,
0.09714151173830032,
-0.009648695588111877,
0.04433329030871391,
0.017441416159272194,
-0.0746534913778305,
-0.0008304978837259114,
-0.007351484149694443,
0.054977234452962875,
-0.015922123566269875,
-0.07937943935394287,
-0.08324998617172241,
-0.007668197620660067,
-0.02523641847074032,
-0.0468604601919651,
0.03312523290514946,
-0.03212722763419151,
0.021373100578784943,
0.04753466323018074,
0.000957822019699961,
-0.06958874315023422,
-0.016745097935199738,
-0.015909360721707344,
-0.0311996228992939,
0.03040957637131214,
0.004888572730123997,
-0.022421300411224365,
0.04437980428338051,
-0.015021327883005142,
-0.012485546991229057,
0.0037392505910247564,
-0.015811488032341003,
-0.00820655096322298,
-0.04135989770293236,
0.0010249308543279767,
-0.03531305491924286,
-0.008875162340700626,
-0.01935979537665844,
-0.00888578500598669,
0.002021823078393936,
0.01168111152946949,
-0.02510988526046276,
0.003063915064558387,
0.009723333641886711,
-0.03675436973571777,
0.03678521513938904,
-0.019651690497994423,
0.0016327836783602834,
0.04181557521224022,
0.001430045929737389,
0.034396130591630936,
0.01749861054122448,
0.0210429597645998,
-0.06880290061235428,
-0.028563110157847404,
-0.06233016401529312,
0.0034061952028423548,
-0.0015238261548802257,
-0.00533914752304554,
0.07341236621141434,
-0.007844067178666592,
-0.04665935039520264,
0.034539539366960526,
0.026430189609527588,
0.0036015119403600693,
0.00726646464318037,
0.03530677780508995,
0.004763786215335131,
-0.028706643730401993,
-0.020072074607014656,
0.005058801732957363,
0.053310878574848175,
0.043709736317396164,
-0.25841861963272095,
-0.024967007339000702,
0.04313317686319351,
0.02419438026845455,
-0.012318218126893044,
0.04712916165590286,
0.06387991458177567,
-0.039621010422706604,
-0.0218448955565691,
-0.020873045548796654,
0.023515017703175545,
0.04741957038640976,
0.036190588027238846,
0.02924627996981144,
-0.04801268130540848,
0.06925566494464874,
0.07385972887277603,
-0.0670974925160408,
0.07083585113286972,
-0.014854197390377522,
0.023845188319683075,
-0.03263315185904503,
0.1403646022081375,
-0.00041409500408917665,
0.01635557785630226,
0.04475335404276848,
0.038565799593925476,
-0.02142913080751896,
-0.05373556166887283,
-0.02439779043197632,
-0.005483011715114117,
0.019777605310082436,
0.11005284637212753,
-0.023889821022748947,
0.013312396593391895,
0.04284904897212982,
0.023155083879828453,
0.023843349888920784,
-0.024112509563565254,
-0.03795991837978363,
-0.017420116811990738,
0.000981667428277433,
-0.09644963592290878,
-0.06872238963842392,
0.1068221852183342,
-0.11749953031539917,
-0.043236952275037766,
-0.06791901588439941,
0.002573437290266156,
0.017019866034388542,
-0.016962070018053055,
-0.0497061125934124,
0.003521100152283907,
0.07074849307537079,
0.030524563044309616,
-0.00014539014955516905,
-0.08348309993743896,
-0.013816745951771736,
-0.03349853679537773,
0.027828941121697426,
0.018057778477668762,
0.034776851534843445,
-0.003812842769548297,
0.07456213235855103,
0.014769918285310268
] |
PMID:27213 | Synergism of triggered luminescence by simultaneous treatment of pH transition and potassium addition in chloroplasts. | KCL-induced luminescence in relation to slow delayed light emission (greater than 3 s) and pH shift-triggered luminescence was studied in preilluminated chloroplasts. An activation pathway for KCl-induced luminescence similar to that for acidbase-triggered luminescence but different from that for delayed light emission is suggested. When the chloroplasts were subjected to a small amount of pH transition together with a simultaneous addition of KCl, a synergistic enhancement of triggered luminescence was observed. The synergism was not observed when the pH transition was increased. The results are interpreted according to the protonation model for stimulated luminescence. | Synergism of triggered luminescence by simultaneous treatment of pH transition and potassium addition in chloroplasts. KCL-induced luminescence in relation to slow delayed light emission (greater than 3 s) and pH shift-triggered luminescence was studied in preilluminated chloroplasts. An activation pathway for KCl-induced luminescence similar to that for acidbase-triggered luminescence but different from that for delayed light emission is suggested. When the chloroplasts were subjected to a small amount of pH transition together with a simultaneous addition of KCl, a synergistic enhancement of triggered luminescence was observed. The synergism was not observed when the pH transition was increased. The results are interpreted according to the protonation model for stimulated luminescence. | [
-0.07969736307859421,
-0.010871010832488537,
0.038938745856285095,
0.053615111857652664,
0.06507671624422073,
-0.006275924853980541,
0.0226626954972744,
-0.016978025436401367,
0.006344750057905912,
-0.00998462364077568,
0.04425758495926857,
-0.06858672946691513,
0.011443548835814,
0.06102892383933067,
0.020697476342320442,
-0.02440461702644825,
-0.0540139339864254,
-0.014065131545066833,
-0.07558724284172058,
-0.03724664822220802,
0.0552571676671505,
-0.0028171385638415813,
0.008545071817934513,
0.00526877585798502,
0.045857686549425125,
0.014446757733821869,
-0.023210741579532623,
-0.01818324625492096,
-0.04567085951566696,
-0.18697701394557953,
-0.03607587888836861,
0.03350000083446503,
-0.006414565723389387,
-0.03356879949569702,
-0.05486154556274414,
-0.028169238939881325,
-0.06051456183195114,
-0.006599367130547762,
-0.05988774821162224,
-0.026046454906463623,
0.0591791570186615,
-0.0020715247374027967,
-0.01498895138502121,
-0.02540217526257038,
-0.019596947357058525,
-0.06373768299818039,
0.02305654250085354,
0.025997595861554146,
0.025336360558867455,
-0.009740852750837803,
0.023500075563788414,
-0.004390725400298834,
-0.0038152034394443035,
-0.018796874210238457,
-0.027328358963131905,
0.04407509043812752,
0.015808703377842903,
0.09624581038951874,
0.05905952304601669,
-0.030749404802918434,
0.04192744195461273,
0.08524566143751144,
-0.16089697182178497,
0.03715161234140396,
0.05115910619497299,
-0.012750314548611641,
-0.026303067803382874,
-0.037309665232896805,
0.07376674562692642,
0.0722312331199646,
-0.026324816048145294,
0.013658522628247738,
-0.0021982472389936447,
0.001348193734884262,
0.03771832212805748,
-0.049368105828762054,
-0.01151721179485321,
0.009820619598031044,
-0.01913689263164997,
0.012821195647120476,
-0.016140548512339592,
-0.00006683451647404581,
-0.042799822986125946,
-0.004568152595311403,
-0.040051743388175964,
0.017928149551153183,
-0.03056376613676548,
-0.029526498168706894,
-0.004503863397985697,
0.04055798053741455,
0.04228509962558746,
-0.061781540513038635,
-0.00796420406550169,
-0.024273058399558067,
-0.02534618228673935,
0.055351629853248596,
0.006509656552225351,
-0.04301100969314575,
-0.0021048581693321466,
0.38578781485557556,
-0.05631435289978981,
0.06940118223428726,
-0.025152424350380898,
0.010348748415708542,
0.02149139903485775,
-0.06716576963663101,
-0.02829565666615963,
0.0230034738779068,
-0.03433613106608391,
-0.020890574902296066,
0.04056108370423317,
-0.03222941979765892,
-0.022970028221607208,
-0.05492670089006424,
-0.011425474658608437,
-0.02227489836513996,
0.07691025733947754,
-0.010594247840344906,
0.02953569032251835,
-0.016419731080532074,
-0.005505034234374762,
0.018897518515586853,
0.04902750253677368,
-0.012254356406629086,
0.05591311305761337,
-0.032476674765348434,
0.011586642824113369,
0.09106293320655823,
0.034316033124923706,
0.021586941555142403,
0.02520899660885334,
0.039787258952856064,
0.015457212924957275,
-0.02158200554549694,
-0.044699527323246,
-0.013982921838760376,
0.04221666231751442,
-0.08184904605150223,
0.03950091078877449,
-0.03286115080118179,
-0.004190657753497362,
-0.033473581075668335,
0.03610967472195625,
-0.07573428750038147,
-0.03211810067296028,
0.07359322160482407,
0.010490363463759422,
0.02922789752483368,
-0.05036332830786705,
0.0420340895652771,
-0.017157629132270813,
-0.017608726397156715,
-0.016610894352197647,
0.03324645385146141,
0.0034621090162545443,
0.003081842325627804,
0.008567203767597675,
0.036032784730196,
0.04617729410529137,
0.015189043246209621,
-0.0763290748000145,
-0.018666300922632217,
0.0002589157084003091,
0.0689663216471672,
0.014859843999147415,
0.0005522704450413585,
0.052788808941841125,
-0.031146224588155746,
-0.018428556621074677,
-0.01723579503595829,
-0.041569869965314865,
0.09861112385988235,
0.03977924585342407,
-0.09738622605800629,
-0.00046016889973543584,
0.031903959810733795,
0.00773299764841795,
0.03289629891514778,
-0.013397674076259136,
-0.019972804933786392,
0.03470011055469513,
-0.014705929905176163,
0.019106421619653702,
0.03622731193900108,
0.06751661747694016,
-0.04538974165916443,
-0.05057145282626152,
0.05853511393070221,
-0.03452294319868088,
0.06526418030261993,
-0.026659101247787476,
-0.0007116480264812708,
-0.04411634802818298,
0.0269168671220541,
-0.02217918075621128,
-0.08244279026985168,
-0.03851357474923134,
-0.030839307233691216,
-0.0059165870770812035,
-0.04394275322556496,
0.06364940851926804,
-0.0032921184320002794,
-0.04438331350684166,
0.017114615067839622,
0.022001588717103004,
-0.01093384250998497,
0.006325308233499527,
0.0770464614033699,
0.06705360114574432,
-0.013132967986166477,
-0.029380347579717636,
-0.05323762446641922,
0.0030548202339559793,
-0.018740270286798477,
-0.06737170368432999,
0.016266148537397385,
-0.007311120163649321,
0.032427188009023666,
0.039961934089660645,
-0.06190218776464462,
0.028941072523593903,
-0.06953177601099014,
-0.0820453017950058,
-0.27745941281318665,
-0.012045818381011486,
0.0022290104534476995,
0.008719583973288536,
0.08943402022123337,
-0.028697015717625618,
0.0038607819005846977,
-0.015068059787154198,
0.016007114201784134,
-0.11864027380943298,
0.03285697102546692,
0.044562239199876785,
-0.003995297942310572,
-0.059245914220809937,
0.05163751542568207,
0.02506437338888645,
-0.011723433621227741,
-0.007439758628606796,
0.020935693755745888,
0.032905980944633484,
0.03846080228686333,
0.0031811033841222525,
-0.0013246353482827544,
-0.012757913209497929,
0.04500296339392662,
0.030919132754206657,
0.10383409261703491,
0.01921367645263672,
-0.02533205971121788,
-0.02672453597187996,
-0.04780516400933266,
0.030984682962298393,
0.002707188716158271,
-0.05681191384792328,
-0.03168046846985817,
0.024229831993579865,
0.049316585063934326,
-0.014010995626449585,
-0.0035676441621035337,
0.013188772834837437,
0.013524872250854969,
-0.012139084748923779,
-0.11993147432804108,
-0.05748193711042404,
0.0016932121943682432,
0.018129443749785423,
0.05295410379767418,
0.07123182713985443,
0.0199142936617136,
-0.016781765967607498,
0.04252522811293602,
-0.023594487458467484,
0.020888345316052437,
-0.015503446571528912,
0.012511090375483036,
-0.04151755943894386,
-0.008743710815906525,
0.047177981585264206,
-0.048821739852428436,
-0.05331701785326004,
0.001689715078100562,
-0.01948176510632038,
-0.016585879027843475,
0.035856761038303375,
0.02529893070459366,
-0.04953175410628319,
-0.03020945005118847,
-0.0023793368600308895,
0.03166048601269722,
-0.02373337186872959,
0.02132364735007286,
0.10478206723928452,
-0.06482241302728653,
0.042416051030159,
0.0486895926296711,
0.07502417266368866,
-0.09256424754858017,
-0.0458332821726799,
-0.0817459374666214,
-0.000606558402068913,
0.013637484051287174,
-0.029306989163160324,
0.02983677387237549,
-0.004122614860534668,
-0.016856804490089417,
0.015363122336566448,
-0.020605674013495445,
-0.004994094837456942,
0.0037943439092487097,
-0.01312935445457697,
-0.031706586480140686,
0.023005561903119087,
0.011753168888390064,
-0.03970382735133171,
0.06870569288730621,
0.014051076956093311,
-0.26323410868644714,
-0.006540008820593357,
0.03821219876408577,
0.023627307265996933,
-0.029069004580378532,
0.08682408183813095,
0.05321948602795601,
-0.029180029407143593,
-0.02397415228188038,
0.07845176011323929,
-0.04574504494667053,
0.0071901013143360615,
-0.002365697640925646,
0.09136667102575302,
0.022920968011021614,
0.044613972306251526,
0.01011373195797205,
-0.09125626087188721,
-0.029230335727334023,
-0.02004750445485115,
-0.023405320942401886,
-0.019869543612003326,
0.15000370144844055,
0.010858684778213501,
0.0026775384321808815,
0.019725533202290535,
0.06536762416362762,
0.030154259875416756,
0.02767856977880001,
-0.013188437558710575,
-0.0059529319405555725,
0.09374630451202393,
-0.03959370404481888,
-0.02479604445397854,
0.03256978839635849,
0.04136468842625618,
-0.011259319260716438,
-0.016327641904354095,
-0.037951476871967316,
0.0018823425052687526,
0.008717581629753113,
0.04913413152098656,
-0.0290977880358696,
-0.017945928499102592,
0.06929749995470047,
-0.034992851316928864,
-0.0016147926216945052,
-0.035450708121061325,
-0.028276171535253525,
-0.025373049080371857,
-0.024677397683262825,
-0.01904558576643467,
0.0527164451777935,
0.09851466864347458,
-0.022327760234475136,
0.02771742269396782,
-0.01660143956542015,
0.006042788736522198,
-0.027358444407582283,
0.035393595695495605,
0.06796886771917343,
-0.019915802404284477,
-0.00032397598261013627,
0.0350247286260128,
0.044994935393333435
] |
PMID:27214 | Interaction of fluorinated ether anesthetics with artificial membranes. | Fluorine-19 nuclear magnetic resonance spectroscopy is applied to the study of the environment of dipalmitoyl phosphatidylcholine-bound fluorinated ether anesthetics (enflurane, fluoroxene and methoxyflurane) both below and above the lipid gel to liquid crystal phase transition temperature. Line widths and spin-lattice relaxation time (T1) measurements are consistent with substantial immobilization of the lipid-bound anesethetic molecules. Heating anesthetic/lipid mixtures above the lipid transition temperature leads to narrowing of the lipid-bound anesthetic fluorine resonances accompanied by little or no change in anesthetic fluorine-19 chemical shifts, suggesting that although the mobility of the bound anesthetic increases at the higher temperature, the nature of the anesthetic-lipid interaction changes little as a result of this phase change. Differential scanning calorimetric studies of the effects of these anesthetics on the phase transition behavior of the phospholipid indicate that the regions of the bilayer in which volatile anesthetics partition at lower concentrations are different from the regions in which they partition at higher concentrations. | Interaction of fluorinated ether anesthetics with artificial membranes. Fluorine-19 nuclear magnetic resonance spectroscopy is applied to the study of the environment of dipalmitoyl phosphatidylcholine-bound fluorinated ether anesthetics (enflurane, fluoroxene and methoxyflurane) both below and above the lipid gel to liquid crystal phase transition temperature. Line widths and spin-lattice relaxation time (T1) measurements are consistent with substantial immobilization of the lipid-bound anesethetic molecules. Heating anesthetic/lipid mixtures above the lipid transition temperature leads to narrowing of the lipid-bound anesthetic fluorine resonances accompanied by little or no change in anesthetic fluorine-19 chemical shifts, suggesting that although the mobility of the bound anesthetic increases at the higher temperature, the nature of the anesthetic-lipid interaction changes little as a result of this phase change. Differential scanning calorimetric studies of the effects of these anesthetics on the phase transition behavior of the phospholipid indicate that the regions of the bilayer in which volatile anesthetics partition at lower concentrations are different from the regions in which they partition at higher concentrations. | [
-0.049774929881095886,
0.029888642951846123,
0.011420397087931633,
-0.01870216429233551,
-0.009267608635127544,
0.01096836756914854,
0.029446713626384735,
0.015929419547319412,
-0.006925906054675579,
-0.11148879677057266,
-0.03846777603030205,
-0.042234160006046295,
0.042666658759117126,
0.008348741568624973,
-0.02321663312613964,
-0.039372630417346954,
-0.010723048821091652,
-0.05009334906935692,
-0.04663984477519989,
-0.005373341031372547,
0.006281108129769564,
-0.0022738289553672075,
-0.00006553350249305367,
-0.030156651511788368,
0.012682175263762474,
0.061114270240068436,
0.015625668689608574,
-0.0071171959862113,
-0.06495977938175201,
-0.2231368124485016,
-0.0050895726308226585,
0.0075685991905629635,
0.05711066722869873,
-0.0687984749674797,
0.012278684414923191,
0.027274811640381813,
0.004029640927910805,
-0.03006279096007347,
-0.01034141331911087,
0.030600594356656075,
0.0519709512591362,
0.02702806331217289,
-0.019097747281193733,
-0.019331758841872215,
0.004053155891597271,
0.009960455819964409,
-0.05560997128486633,
0.0052218190394341946,
0.10636549443006516,
0.033258941024541855,
0.0334751233458519,
-0.02586328610777855,
0.016483569517731667,
0.04641951248049736,
0.008847802877426147,
-0.0055076791904866695,
-0.02963382750749588,
-0.017698585987091064,
-0.013597539626061916,
0.0045150276273489,
0.026644282042980194,
0.02176165208220482,
-0.15612322092056274,
0.13568156957626343,
-0.0013264169683679938,
-0.06297201663255692,
0.058500781655311584,
-0.05862930789589882,
0.07690877467393875,
0.02376375161111355,
-0.04565858095884323,
-0.004961180500686169,
-0.04060196131467819,
-0.03806721791625023,
0.02227635309100151,
-0.024500291794538498,
0.016409877687692642,
0.004676072858273983,
0.03040284663438797,
-0.04763493686914444,
-0.01378600299358368,
-0.044311895966529846,
-0.05157775804400444,
-0.04196016490459442,
-0.024683963507413864,
0.04148498922586441,
0.08079052716493607,
-0.046946533024311066,
0.023336848244071007,
0.050164978951215744,
-0.02551351860165596,
-0.015356791205704212,
0.001279982621781528,
-0.0026527438312768936,
-0.023531660437583923,
0.05981339141726494,
0.012511474080383778,
0.03965415060520172,
0.04838721826672554,
0.345561683177948,
-0.02768026664853096,
0.022711312398314476,
-0.03895535320043564,
-0.025389326736330986,
-0.03030400164425373,
-0.02744215354323387,
0.049811508506536484,
-0.028440425172448158,
0.02446880005300045,
0.03829837962985039,
0.016904499381780624,
-0.0479423850774765,
0.053161486983299255,
-0.04745436832308769,
-0.03000236675143242,
-0.01038777269423008,
0.08526933193206787,
0.005768964998424053,
0.0015394395450130105,
0.00943194329738617,
-0.017209477722644806,
-0.02449200488626957,
-0.0013672473141923547,
-0.008159740827977657,
0.050667375326156616,
-0.0009685317636467516,
-0.03206867352128029,
0.09582363814115524,
-0.006840127054601908,
-0.010257977060973644,
0.02829810418188572,
0.042220260947942734,
0.009326581843197346,
-0.030175326392054558,
-0.03209151327610016,
0.052947964519262314,
0.008663217537105083,
-0.026847735047340393,
0.03548024222254753,
0.0018014188390225172,
0.006111927330493927,
-0.017318202182650566,
-0.015380299650132656,
-0.096468985080719,
-0.0387091226875782,
0.07075435668230057,
-0.03603657707571983,
-0.02127409353852272,
-0.058562152087688446,
0.04866177588701248,
0.0020180700812488794,
0.03172483295202255,
-0.09411786496639252,
0.03844396397471428,
-0.0019346748013049364,
0.01475354190915823,
0.022359775379300117,
-0.007171059027314186,
-0.033299077302217484,
-0.032302126288414,
-0.04841269552707672,
0.0118418512865901,
0.02388489618897438,
0.0986478254199028,
-0.041875019669532776,
-0.030511725693941116,
-0.025874581187963486,
0.014104900881648064,
-0.02883332408964634,
0.023057831451296806,
0.016514116898179054,
-0.06788840889930725,
-0.012678113766014576,
-0.04811222478747368,
-0.015068906359374523,
-0.032810408622026443,
0.034211415797472,
0.018619028851389885,
-0.013307440094649792,
-0.01465647667646408,
-0.024079134687781334,
-0.0067925648763775826,
-0.006919361185282469,
0.025598617270588875,
0.03409923240542412,
-0.0001831576955737546,
-0.0578107014298439,
0.013147360645234585,
-0.019475331529974937,
0.016912760213017464,
-0.02858302928507328,
0.010958194732666016,
-0.06014164537191391,
0.05473616346716881,
-0.05781467631459236,
-0.03967198729515076,
-0.04171663895249367,
0.023778526112437248,
-0.027767404913902283,
0.009423677809536457,
0.12190716713666916,
-0.023624304682016373,
-0.015038975514471531,
0.01871359534561634,
0.054889220744371414,
0.01598929800093174,
0.01710432395339012,
0.052571430802345276,
0.054178107529878616,
0.010185424238443375,
-0.02634294331073761,
0.004211851395666599,
-0.018513359129428864,
-0.051133207976818085,
-0.04204533249139786,
-0.010316460393369198,
0.01943950355052948,
-0.012762926518917084,
0.0010608409065753222,
-0.03855520486831665,
0.08812536299228668,
-0.03911646455526352,
-0.04122842848300934,
-0.34321776032447815,
0.005488663446158171,
-0.02850523591041565,
-0.003358915913850069,
0.06978859752416611,
-0.0004937972989864647,
0.047497447580099106,
-0.025213388726115227,
0.004166492260992527,
-0.049855850636959076,
-0.022826090455055237,
0.027877142652869225,
-0.09157703071832657,
-0.017304543405771255,
-0.0032797472085803747,
-0.0643211230635643,
0.06546024233102798,
-0.041786231100559235,
0.041336141526699066,
0.014720224775373936,
-0.029111003503203392,
0.016772737726569176,
-0.08184107393026352,
-0.02372954785823822,
0.05203407257795334,
-0.006472981069236994,
0.0997542068362236,
-0.04189271107316017,
-0.01536430325359106,
-0.009819841012358665,
0.02476835809648037,
0.027757717296481133,
0.0032586692832410336,
-0.06011064350605011,
-0.0007306344341486692,
-0.02590775676071644,
0.0597541406750679,
-0.0046021947637200356,
0.05031931400299072,
-0.030281592160463333,
-0.01912512257695198,
0.009907852858304977,
-0.023829923942685127,
-0.0026197240222245455,
-0.05279923230409622,
-0.042642027139663696,
-0.03587203100323677,
-0.05610653758049011,
-0.033829689025878906,
0.008757485076785088,
0.04608343541622162,
0.051918480545282364,
-0.01189310010522604,
-0.03888803720474243,
0.005391745828092098,
-0.03671564161777496,
0.01303769275546074,
-0.00850615557283163,
-0.014286535792052746,
0.0362873449921608,
0.002651773625984788,
0.0595206692814827,
0.06283381581306458,
-0.01206235121935606,
-0.005050932988524437,
0.0019030955154448748,
0.0444193072617054,
-0.013687473721802235,
0.05905401334166527,
0.018702087923884392,
0.00995582528412342,
0.140525221824646,
-0.03640179708600044,
-0.009782437235116959,
0.10751259326934814,
0.01980467699468136,
-0.02444148249924183,
-0.0027705999091267586,
-0.04605090245604515,
0.0770404040813446,
0.06512617319822311,
0.0379728227853775,
0.048088669776916504,
-0.05691544711589813,
-0.040146421641111374,
0.03235744312405586,
0.04094405472278595,
-0.03457830846309662,
0.04545234888792038,
0.0011632213136181235,
0.04113200679421425,
0.01627263054251671,
-0.012869100086390972,
-0.06434501707553864,
0.052966922521591187,
0.03339933976531029,
-0.2618798017501831,
0.0013046172680333257,
0.04716821759939194,
0.06676971167325974,
0.003129820805042982,
-0.021991200745105743,
0.00047696116962470114,
0.001042580814100802,
-0.10377447307109833,
-0.01685955934226513,
-0.035333164036273956,
0.06627851724624634,
0.02271399088203907,
0.06905077397823334,
0.00463611725717783,
0.033176641911268234,
0.07485593110322952,
-0.021472610533237457,
-0.00464990921318531,
-0.06647704541683197,
-0.0001942391972988844,
-0.03735314682126045,
0.14075185358524323,
-0.003981238231062889,
-0.02053765021264553,
0.07791461795568466,
0.002002607099711895,
0.027766525745391846,
-0.0004476489848457277,
0.02254013903439045,
0.03020416758954525,
-0.008252418600022793,
0.022728407755494118,
-0.034895919263362885,
0.023965410888195038,
0.06506189703941345,
-0.0325293205678463,
0.02700527012348175,
0.0013864213833585382,
0.008629546500742435,
0.050355464220047,
0.03850507736206055,
-0.04757881909608841,
-0.01416871789842844,
0.033150311559438705,
-0.06615035980939865,
0.017942573875188828,
0.035588834434747696,
-0.009315057657659054,
0.019832182675600052,
0.012759626843035221,
0.03376033529639244,
-0.0164522435516119,
0.023071618750691414,
0.07720872759819031,
0.008741892874240875,
0.022345812991261482,
-0.055165957659482956,
0.014179878868162632,
0.03921332582831383,
-0.026743492111563683,
-0.02596740424633026,
-0.03383807837963104,
0.02301476150751114,
0.033235009759664536
] |
PMID:27216 | Characterization of two poly(A) polymerases from cultured hamster fibroblasts. | The enzymatic and physiochemical properties of poly(A) polymerases IIA and IIB from cultured hamster fibroblasts were investigated. The enzymes show an absolute requirement for Mn2+ as the divalent ion. Although Mg2+ alone is inactive, maximum activity is observed in the presence of both Mn2+ and Mg2+. An optimal pH of approx. 8 is found for polymerases IIA and IIB. The enzymes, however, differ somewhat in the pH curves as well as in the Mn2+ and Mg2+ concentration curves. Poly(A) polymerases IIA and IIB have an isoelectric point of about 6 and a sedimentation coefficient of 3.5--4 S. The molecular weights, obtained by gel filtration chromatography, are 145 000 and 155 000 for enzymes IIA and IIB, respectively. Poly(A) polymerases IIA and IIB can utilize a variety of natural and synthetic RNAs as well as DNA as primers. Poly(A) polymerase IIA is saturated at much lower concentrations of primer than enzyme IIB. On the other hand, the chain length of the product synthesized by polymerase IIA is independent of the primer concentration, whereas, with polymerase IIB, the length of the product decreases when the concentration of RNA is increased. | Characterization of two poly(A) polymerases from cultured hamster fibroblasts. The enzymatic and physiochemical properties of poly(A) polymerases IIA and IIB from cultured hamster fibroblasts were investigated. The enzymes show an absolute requirement for Mn2+ as the divalent ion. Although Mg2+ alone is inactive, maximum activity is observed in the presence of both Mn2+ and Mg2+. An optimal pH of approx. 8 is found for polymerases IIA and IIB. The enzymes, however, differ somewhat in the pH curves as well as in the Mn2+ and Mg2+ concentration curves. Poly(A) polymerases IIA and IIB have an isoelectric point of about 6 and a sedimentation coefficient of 3.5--4 S. The molecular weights, obtained by gel filtration chromatography, are 145 000 and 155 000 for enzymes IIA and IIB, respectively. Poly(A) polymerases IIA and IIB can utilize a variety of natural and synthetic RNAs as well as DNA as primers. Poly(A) polymerase IIA is saturated at much lower concentrations of primer than enzyme IIB. On the other hand, the chain length of the product synthesized by polymerase IIA is independent of the primer concentration, whereas, with polymerase IIB, the length of the product decreases when the concentration of RNA is increased. | [
-0.10488671064376831,
-0.018913228064775467,
-0.039878759533166885,
-0.011075619608163834,
0.019067227840423584,
0.037541020661592484,
0.017360681667923927,
0.05841531231999397,
-0.0312928669154644,
0.001634444110095501,
0.05835568904876709,
-0.050650738179683685,
-0.0006133596180006862,
-0.011562827974557877,
-0.00853418093174696,
0.025357704609632492,
-0.010085339657962322,
-0.056482020765542984,
-0.02740514650940895,
0.04939468577504158,
0.028093744069337845,
-0.018710123375058174,
0.056742433458566666,
-0.026969868689775467,
0.007808141876012087,
0.09390110522508621,
-0.01503290981054306,
0.01685560680925846,
-0.06088508665561676,
-0.22670575976371765,
0.01704353280365467,
-0.04806030914187431,
0.0538782961666584,
-0.003991670440882444,
-0.009530607610940933,
-0.008198337629437447,
-0.03624153882265091,
-0.028510911390185356,
-0.03673531860113144,
0.027047961950302124,
0.03973430022597313,
0.005621297750622034,
0.011680024676024914,
0.0061561595648527145,
0.011651511304080486,
-0.012300928123295307,
-0.04142829403281212,
-0.0019005554495379329,
0.03882420063018799,
0.043025851249694824,
0.005690532736480236,
0.0006328815943561494,
0.0038992143236100674,
0.03951401636004448,
-0.017869481816887856,
-0.0086330845952034,
-0.010253244079649448,
0.07415534555912018,
0.0007478906190954149,
0.013770190998911858,
-0.010498102754354477,
-0.005615882575511932,
-0.12166443467140198,
0.07222213596105576,
0.06528090685606003,
0.036112356930971146,
-0.06471563130617142,
-0.06790724396705627,
-0.033594075590372086,
0.02622302435338497,
-0.08207353949546814,
0.057042766362428665,
0.013139458373188972,
0.025800419971346855,
-0.030301198363304138,
0.008133267983794212,
0.006396397016942501,
-0.007768732961267233,
0.07919862121343613,
0.008706728927791119,
-0.06226392462849617,
-0.05029810220003128,
-0.004546539857983589,
-0.025851130485534668,
-0.033607229590415955,
0.07360462844371796,
0.009649849496781826,
-0.007791122887283564,
0.03196808323264122,
0.01772766374051571,
-0.00978303886950016,
-0.02199234813451767,
0.02706787921488285,
-0.015092882327735424,
-0.0710378885269165,
0.0876815915107727,
0.04832139238715172,
0.0013351828092709184,
0.06102718785405159,
0.38423123955726624,
-0.019973943009972572,
0.026673834770917892,
-0.05631084367632866,
-0.04243714362382889,
-0.05096987262368202,
-0.023787906393408775,
-0.025604186579585075,
-0.006217672489583492,
0.045347005128860474,
0.03350241109728813,
0.05619547888636589,
0.03178216144442558,
-0.01451327744871378,
-0.03868342936038971,
-0.00004294026439310983,
-0.009760246612131596,
0.014316760003566742,
0.020992474630475044,
-0.039854977279901505,
-0.015459329821169376,
-0.04736550152301788,
-0.06811469793319702,
0.020646777004003525,
0.011664321646094322,
0.02959945797920227,
-0.026937242597341537,
-0.012950442731380463,
0.012335485778748989,
0.027614764869213104,
0.031719107180833817,
0.029357444494962692,
0.03606940060853958,
-0.028089873492717743,
0.02062402479350567,
0.038568541407585144,
-0.07923702895641327,
-0.00743046635761857,
0.019274115562438965,
0.01718246378004551,
-0.025504248216748238,
-0.010476217605173588,
-0.04958873242139816,
-0.042257096618413925,
-0.008541072718799114,
-0.03996800258755684,
0.038147639483213425,
-0.018220683559775352,
0.07453186064958572,
0.002299971180036664,
-0.022056683897972107,
0.027056602761149406,
0.06354673951864243,
-0.0060468814335763454,
0.04551585018634796,
0.04273444041609764,
0.0021864373702555895,
0.01528142113238573,
-0.06636738032102585,
-0.0021321577951312065,
-0.012883741408586502,
-0.07253465056419373,
-0.017573634162545204,
-0.027873829007148743,
0.10367567837238312,
0.008267289027571678,
-0.05654729902744293,
-0.034223273396492004,
0.014023790135979652,
0.05943971499800682,
-0.013877549208700657,
0.055526211857795715,
-0.02154051698744297,
-0.00019603667897172272,
-0.0008220477029681206,
-0.033892594277858734,
-0.05394750088453293,
-0.02895319275557995,
0.019229313358664513,
0.02962072193622589,
0.02466052584350109,
0.012195615097880363,
-0.01954728364944458,
-0.00682900520041585,
0.009821692481637001,
0.02964661456644535,
0.011999377980828285,
-0.052835915237665176,
0.0470326691865921,
0.000933108851313591,
0.03713102638721466,
0.016967521980404854,
0.024321777746081352,
-0.0818224772810936,
0.05065177008509636,
-0.014077415689826012,
-0.01695212535560131,
-0.0023142921272665262,
0.08499012142419815,
-0.030530132353305817,
-0.03741557151079178,
0.11027103662490845,
0.011899631470441818,
0.0313682034611702,
0.08636748045682907,
-0.013794302940368652,
-0.010227018967270851,
0.03056342341005802,
0.054867468774318695,
0.018168911337852478,
0.008759654127061367,
-0.07289183884859085,
-0.0023414739407598972,
-0.08801908046007156,
-0.05153905972838402,
-0.0764414593577385,
0.017958015203475952,
0.025331227108836174,
0.05569460242986679,
0.04819025844335556,
0.04283514618873596,
0.016283197328448296,
-0.06297938525676727,
-0.06792529672384262,
-0.28524142503738403,
0.003633422078564763,
-0.020591603592038155,
-0.0122173260897398,
0.06327935308218002,
-0.013399278745055199,
0.006344955414533615,
-0.001069902442395687,
-0.005331093445420265,
-0.05635182186961174,
-0.01190735213458538,
0.035175614058971405,
-0.03248074650764465,
-0.03137017786502838,
-0.0475158616900444,
0.08367367088794708,
0.03765074908733368,
0.0055761756375432014,
-0.014560133218765259,
0.01618872955441475,
-0.008272169157862663,
0.008273109793663025,
0.005800791550427675,
0.06748856604099274,
0.05706014484167099,
0.038069505244493484,
0.11580926924943924,
0.00418610405176878,
-0.00673084519803524,
0.016154780983924866,
0.035636868327856064,
0.10124485194683075,
-0.05288976803421974,
-0.07959813624620438,
-0.02570008486509323,
-0.022048255428671837,
-0.04057619348168373,
-0.00723009230569005,
-0.0005333349690772593,
0.012812945060431957,
0.03574515879154205,
0.061684977263212204,
-0.020433086901903152,
-0.03482363745570183,
-0.007008655462414026,
-0.0012406168971210718,
0.004220456350594759,
0.036300595849752426,
-0.0007162308320403099,
-0.031879544258117676,
0.023839721456170082,
-0.03131997212767601,
0.01802908070385456,
-0.06366512924432755,
0.02534164860844612,
0.018600501120090485,
-0.012369356118142605,
0.03343285620212555,
-0.08873870223760605,
0.0033607908990234137,
-0.005408229772001505,
-0.02515277825295925,
0.005280839744955301,
0.08888254314661026,
-0.008439767174422741,
0.00619453564286232,
0.0313052274286747,
-0.026417016983032227,
0.052007757127285004,
-0.020557953044772148,
-0.0010101511143147945,
0.0911841094493866,
0.002373794559389353,
-0.00881166197359562,
0.054798997938632965,
0.03888756036758423,
-0.05363202840089798,
-0.05674049258232117,
-0.07302369177341461,
-0.041363831609487534,
0.031279489398002625,
-0.020747819915413857,
0.06735765188932419,
-0.018423693254590034,
-0.006786058656871319,
0.02041664905846119,
0.05117812752723694,
0.009981234557926655,
-0.009414810687303543,
0.001787984394468367,
-0.007534671574831009,
-0.005994442384690046,
-0.012474794872105122,
-0.0018244244856759906,
-0.030859556049108505,
0.01335304044187069,
-0.27502167224884033,
-0.0716126337647438,
0.023956719785928726,
0.04744853079319,
-0.1075497567653656,
0.03897436335682869,
0.01652894727885723,
-0.032242532819509506,
0.0025893428828567266,
0.04770118370652199,
0.02336660958826542,
0.06509432196617126,
0.024149268865585327,
0.050520382821559906,
-0.025006499141454697,
0.034821610897779465,
0.046597763895988464,
-0.0060854763723909855,
0.02473534271121025,
-0.08795593678951263,
-0.03334422409534454,
-0.05291637033224106,
0.1344653218984604,
-0.049002986401319504,
0.012264641933143139,
0.007924673147499561,
-0.008432249538600445,
0.0197413582354784,
-0.044909827411174774,
-0.03337767720222473,
-0.004562562331557274,
0.03127088397741318,
0.09430377185344696,
-0.03274432197213173,
0.01393088884651661,
0.04301809147000313,
-0.03336412087082863,
0.005259732715785503,
-0.03167424350976944,
-0.019006144255399704,
0.03141868859529495,
-0.008317610248923302,
-0.11246532201766968,
-0.021640868857502937,
0.022749679163098335,
-0.03720280900597572,
-0.05932580307126045,
-0.036788035184144974,
-0.021963275969028473,
0.011268817819654942,
-0.05684144049882889,
-0.008075674064457417,
0.04056769609451294,
0.009213621728122234,
-0.02996513806283474,
-0.0321212075650692,
-0.0151319345459342,
-0.016402043402194977,
0.03791681304574013,
-0.0040833246894180775,
0.05239551514387131,
0.008152744732797146,
-0.008492938242852688,
0.10122577100992203,
0.003662707516923547
] |
PMID:27217 | The phosphorylation of ribosomal proteins L14 and S3 in Krebs II ascites cells. | Krebs II ascites cells incubated in Earle's saline (lacking glucose and amino acids) contain ribosomes with proteins S6 and Lgamma phosphorylated, as do ascites cells grown in the peritonea of mice or hamster fibroblasts grown in Eagle's medium. When ascites cells were incubated in Eagle's medium (containing glucose and amino acids) there was extensive glycolysis, producing very acidic conditions, and ribosomal proteins S3 and L14 became phosphorylated whereas Lgamma became dephosphorylated. This altered pattern of phosphorylation could not be produced merely by incubating ascites cells in Earle's saline at a decreased pH, but a rather similar pattern was produced when Earle's saline was supplemented with amino acids (but with glucose still omitted). These results suggest that depriving ascites cells of glucose may induce the synthesis of a protein (or proteins), necessary for alteration of the pattern of phosphorylation of the ribosomal proteins. | The phosphorylation of ribosomal proteins L14 and S3 in Krebs II ascites cells. Krebs II ascites cells incubated in Earle's saline (lacking glucose and amino acids) contain ribosomes with proteins S6 and Lgamma phosphorylated, as do ascites cells grown in the peritonea of mice or hamster fibroblasts grown in Eagle's medium. When ascites cells were incubated in Eagle's medium (containing glucose and amino acids) there was extensive glycolysis, producing very acidic conditions, and ribosomal proteins S3 and L14 became phosphorylated whereas Lgamma became dephosphorylated. This altered pattern of phosphorylation could not be produced merely by incubating ascites cells in Earle's saline at a decreased pH, but a rather similar pattern was produced when Earle's saline was supplemented with amino acids (but with glucose still omitted). These results suggest that depriving ascites cells of glucose may induce the synthesis of a protein (or proteins), necessary for alteration of the pattern of phosphorylation of the ribosomal proteins. | [
-0.05696595087647438,
-0.06004934012889862,
-0.04076283052563667,
-0.0025067729875445366,
0.0026598447002470493,
-0.030085032805800438,
0.029397523030638695,
-0.00555312167853117,
-0.06320032477378845,
0.0266988854855299,
0.07712852954864502,
-0.08169759064912796,
-0.021115435287356377,
0.02074883133172989,
-0.02059757336974144,
-0.007142696063965559,
-0.03912968933582306,
0.009857632219791412,
-0.09833760559558868,
-0.0018000483978539705,
-0.016647860407829285,
0.004618034698069096,
-0.002376761520281434,
-0.005021632183343172,
0.02777702733874321,
0.04228103905916214,
0.0033708016853779554,
0.02227725274860859,
-0.03398486599326134,
-0.18681544065475464,
0.03668728843331337,
-0.04746202751994133,
0.01843828521668911,
-0.07142738252878189,
-0.01765614189207554,
0.007843385450541973,
-0.016801703721284866,
0.03315938264131546,
-0.05723714455962181,
0.06928647309541702,
-0.0012189665576443076,
0.014725558459758759,
0.010345043614506721,
0.008804400451481342,
0.049218010157346725,
-0.059544794261455536,
-0.03897834196686745,
-0.05262379348278046,
0.07149438560009003,
0.0343906544148922,
-0.05015294626355171,
-0.02638627029955387,
0.025038203224539757,
0.04445718601346016,
0.006123031955212355,
0.005744210444390774,
0.0035695673432201147,
0.04494612663984299,
0.03781631216406822,
-0.0268431156873703,
-0.006336034741252661,
0.0051923030987381935,
-0.12756000459194183,
0.029936423525214195,
0.06288297474384308,
-0.011510637588799,
-0.019325923174619675,
-0.0558273121714592,
0.04930877685546875,
0.029446398839354515,
-0.043182142078876495,
0.0358298197388649,
0.04251506179571152,
0.048917192965745926,
0.030945109203457832,
-0.038592610508203506,
-0.060252778232097626,
0.03918856382369995,
0.043911196291446686,
0.02395596355199814,
-0.034623581916093826,
-0.056859757751226425,
-0.005949084181338549,
0.03432945907115936,
-0.055821117013692856,
-0.011430148035287857,
0.0000652013550279662,
-0.04444323480129242,
0.016582457348704338,
0.017081517726182938,
-0.04135478287935257,
-0.08358120918273926,
-0.006564764305949211,
0.021480169147253036,
-0.09593929350376129,
0.014496526680886745,
-0.017833499237895012,
-0.012536919675767422,
0.06683409214019775,
0.36100733280181885,
-0.04449674114584923,
0.056063827127218246,
-0.05664651468396187,
0.018800359219312668,
0.005595813505351543,
-0.08118453621864319,
-0.012238620780408382,
0.01323770359158516,
0.02399338223040104,
0.1058376282453537,
0.06573538482189178,
-0.019953584298491478,
-0.004635956138372421,
-0.009504707530140877,
0.019495774060487747,
0.023914501070976257,
0.0017214007675647736,
0.01844620890915394,
-0.08043506741523743,
0.06701678782701492,
-0.011154010891914368,
0.057508524507284164,
0.016661496832966805,
-0.03609466925263405,
0.07063426077365875,
0.049437738955020905,
-0.003971508704125881,
0.06577591598033905,
0.0689452514052391,
0.025624558329582214,
0.04864059388637543,
0.0806378722190857,
-0.00015085643099155277,
0.04450713098049164,
0.028499724343419075,
-0.011416075751185417,
0.026034977287054062,
-0.0868704691529274,
0.0034781016875058413,
-0.031092427670955658,
-0.029900329187512398,
-0.0798017680644989,
-0.03947403281927109,
-0.025296205654740334,
0.007853356190025806,
0.11183889955282211,
-0.002988215768709779,
0.05790816619992256,
-0.035580702126026154,
-0.02467411756515503,
-0.02152133174240589,
0.03809023275971413,
0.006824802607297897,
0.016982272267341614,
0.0005760702188126743,
0.03136588633060455,
-0.034449923783540726,
0.014454210177063942,
0.030297577381134033,
-0.06479553878307343,
-0.03837186098098755,
-0.037451453506946564,
0.03732188418507576,
0.0396682433784008,
0.029369456693530083,
0.013118245638906956,
-0.004255206789821386,
0.03719739615917206,
0.0005584437749348581,
-0.02253519929945469,
0.011793173849582672,
-0.0029901983216404915,
0.004987056832760572,
-0.05171516537666321,
0.029212214052677155,
0.0014024805277585983,
0.021670691668987274,
-0.008228312246501446,
0.038929712027311325,
-0.00238220626488328,
-0.006482601631432772,
-0.03267403319478035,
-0.011815530247986317,
0.027924906462430954,
-0.015484504401683807,
-0.07857676595449448,
-0.07022236287593842,
0.01937180943787098,
-0.04621032997965813,
0.06392025202512741,
-0.01874341256916523,
0.018875421956181526,
-0.07478192448616028,
-0.033491965383291245,
0.004000807646661997,
0.008129559457302094,
0.027094626799225807,
-0.019237512722611427,
-0.014742671512067318,
-0.0016046161763370037,
0.08920174837112427,
0.03033679537475109,
-0.038749027997255325,
0.028672337532043457,
-0.044896725565195084,
0.0015890231588855386,
0.01550057902932167,
0.03348217532038689,
-0.0027051595970988274,
-0.0023068615701049566,
-0.03522690758109093,
-0.03837606683373451,
-0.0995342954993248,
0.024669267237186432,
-0.019954998046159744,
0.05708405375480652,
-0.013029245659708977,
-0.01630925014615059,
0.0135498670861125,
-0.025075141340494156,
0.06907868385314941,
-0.04327835515141487,
-0.07396240532398224,
-0.31531253457069397,
0.0313982330262661,
0.07510709017515182,
-0.03934966027736664,
0.04095480218529701,
-0.028682535514235497,
0.006101767998188734,
0.05517195537686348,
-0.012638458982110023,
-0.0474819578230381,
0.027304399758577347,
0.04559769108891487,
-0.014529156498610973,
-0.03663710504770279,
0.004389370325952768,
0.002005462534725666,
0.011952506378293037,
-0.028990374878048897,
0.03444724529981613,
0.012577710673213005,
0.0027811117470264435,
0.020945383235812187,
0.002519159112125635,
0.0018373391358181834,
0.02850227989256382,
-0.035265982151031494,
0.11867783218622208,
0.06221979856491089,
0.04079655557870865,
-0.027194324880838394,
0.05967790260910988,
0.027004089206457138,
0.052136827260255814,
-0.08652763068675995,
0.0024301044177263975,
0.016261884942650795,
0.009223819710314274,
0.0030863347928971052,
0.012997865676879883,
0.0035447950940579176,
-0.02204899489879608,
-0.026622796431183815,
-0.06338170170783997,
-0.0008134828531183302,
-0.019282901659607887,
0.007153717800974846,
0.03289764001965523,
0.040150824934244156,
0.055911049246788025,
-0.030606672167778015,
0.007807356305420399,
0.004490130580961704,
0.002158957999199629,
-0.03728216141462326,
0.002270996104925871,
0.013508153147995472,
-0.01868937537074089,
-0.036257658153772354,
-0.07057396322488785,
-0.0010567267891019583,
-0.01811346597969532,
0.011318465694785118,
-0.07834178954362869,
0.03545517474412918,
0.008349618874490261,
0.01507964264601469,
-0.045285388827323914,
0.0023720397148281336,
0.043045077472925186,
0.0028220433741807938,
0.0320221446454525,
0.06720656901597977,
-0.04232832044363022,
0.05345255509018898,
0.06299198418855667,
0.01917649619281292,
-0.022051380947232246,
-0.03583623096346855,
-0.07515230774879456,
0.0065163192339241505,
0.03712846338748932,
-0.04822120815515518,
0.03896216303110123,
0.005894452799111605,
-0.022633463144302368,
0.05484740436077118,
-0.024917954578995705,
-0.01768103428184986,
-0.0022619995288550854,
-0.008567086420953274,
-0.039437439292669296,
-0.03742474690079689,
-0.042111411690711975,
-0.06607943028211594,
0.07257523387670517,
0.0012225807877257466,
-0.2645685076713562,
0.011529944837093353,
0.0161292664706707,
0.038926128298044205,
-0.01747710630297661,
0.02741236984729767,
0.018269186839461327,
-0.03969293460249901,
0.012707668356597424,
0.04718123748898506,
0.06675280630588531,
0.013789585791528225,
0.040660202503204346,
0.005370618775486946,
-0.010636466555297375,
0.028754886239767075,
0.05975060909986496,
-0.06853467226028442,
0.053225498646497726,
-0.05104893818497658,
0.019323065876960754,
-0.03278622776269913,
0.16674451529979706,
0.022184059023857117,
-0.009151349775493145,
0.05480140075087547,
-0.019891172647476196,
0.0673225075006485,
0.004981911741197109,
0.03514178469777107,
-0.001280323718674481,
0.017684873193502426,
0.05149132385849953,
0.05736623331904411,
0.0040697320364415646,
0.009926497004926205,
-0.01626736670732498,
0.04588865861296654,
-0.013994989916682243,
0.04014003649353981,
-0.02041548304259777,
0.018326731398701668,
-0.08884784579277039,
-0.048192836344242096,
0.0835033729672432,
-0.06944069266319275,
-0.0343380942940712,
-0.12127124518156052,
0.03337870538234711,
0.002614170080050826,
-0.02933020330965519,
-0.01654703915119171,
-0.012741370126605034,
0.035005323588848114,
-0.02105606161057949,
0.005242680665105581,
-0.011335965245962143,
0.006987227126955986,
-0.02686266414821148,
0.036612171679735184,
0.055198002606630325,
-0.05553076043725014,
0.012386631220579147,
0.10740474611520767,
-0.028161196038126945
] |
PMID:27218 | Protein methylation in animal cells. I. Purification and properties of S-adenosyl-L-methionine:protein (arginine) N-methyltransferase from Krebs II ascites cells. | 1. A protein methylase which specifically transfers methyl groups from S-adenosyl-L-methionine to arginine residues of histones has been substantially purified from Krebs II ascites cells. The purified enzyme was obtained free of contamination by other protein methyl transferases specific for carboxyl and lysine residues. This latter activity copurified with the present enzyme until advanced stages of purification. 2. The purified enzyme does not require any divalent cation for maximum activity. It is inhibited by ionic strength, N-ethylmaleimide and S-adenosyl-L-homocysteine. It has an apparent molecular weight on gel filtration of approx. 5 . 10(5). A Km value for S-adenosyl-L-methionine of 2.5 . 10(-6) M was determined, while the dissociation constant Ki for S-adenosyl-L-homocysteine, which acts as a competitor, was 1.4 . 10(-6) M. | Protein methylation in animal cells. I. Purification and properties of S-adenosyl-L-methionine:protein (arginine) N-methyltransferase from Krebs II ascites cells. 1. A protein methylase which specifically transfers methyl groups from S-adenosyl-L-methionine to arginine residues of histones has been substantially purified from Krebs II ascites cells. The purified enzyme was obtained free of contamination by other protein methyl transferases specific for carboxyl and lysine residues. This latter activity copurified with the present enzyme until advanced stages of purification. 2. The purified enzyme does not require any divalent cation for maximum activity. It is inhibited by ionic strength, N-ethylmaleimide and S-adenosyl-L-homocysteine. It has an apparent molecular weight on gel filtration of approx. 5 . 10(5). A Km value for S-adenosyl-L-methionine of 2.5 . 10(-6) M was determined, while the dissociation constant Ki for S-adenosyl-L-homocysteine, which acts as a competitor, was 1.4 . 10(-6) M. | [
-0.05903490260243416,
-0.00618410250172019,
-0.09865172207355499,
-0.021266339346766472,
-0.0439595952630043,
0.007233573589473963,
0.07059194892644882,
-0.016718801110982895,
-0.014869450591504574,
0.04837624356150627,
0.0020984529983252287,
-0.10469137132167816,
0.033099427819252014,
0.006896939128637314,
0.010565578937530518,
0.009129399433732033,
0.0017294896533712745,
0.04699837043881416,
-0.026179777458310127,
-0.022838380187749863,
0.005282319616526365,
0.02043374627828598,
0.010066733695566654,
-0.017367098480463028,
0.045075416564941406,
0.018983397632837296,
-0.02166738174855709,
0.011242433451116085,
-0.05955133214592934,
-0.2200508564710617,
0.011542157270014286,
-0.027267392724752426,
-0.0015772144543007016,
-0.03409557417035103,
-0.0013170314487069845,
0.04179690033197403,
-0.017754295840859413,
-0.0007936590700410306,
-0.038866717368364334,
0.05427084118127823,
0.025408606976270676,
-0.013119245879352093,
0.03906121850013733,
-0.03299802169203758,
0.042673759162425995,
0.01201271079480648,
-0.01905975304543972,
-0.03973718360066414,
0.040353357791900635,
0.006570646073669195,
-0.0012526101199910045,
0.0348564013838768,
0.010103272274136543,
0.06164021044969559,
0.02587895654141903,
0.06926801800727844,
0.008050673641264439,
0.06522826850414276,
0.0015478994464501739,
0.016867229714989662,
-0.0011112812208011746,
-0.015149193815886974,
-0.1077936440706253,
0.09645161032676697,
0.033263061195611954,
0.029916884377598763,
-0.00001374417024635477,
-0.0350910909473896,
0.06526519358158112,
-0.003207489848136902,
-0.02183239720761776,
0.026305582374334335,
0.015484395436942577,
0.03822316601872444,
0.04116937145590782,
0.008033707737922668,
-0.00813507754355669,
-0.01622038148343563,
0.06406985223293304,
0.006756401155143976,
-0.03105759434401989,
-0.019414838403463364,
-0.000649230380076915,
-0.04417460039258003,
-0.005757863633334637,
-0.020206572487950325,
0.01400433387607336,
-0.012226929888129234,
0.03931061550974846,
0.04406605288386345,
-0.030887579545378685,
-0.07075793296098709,
0.0200971607118845,
-0.02419031597673893,
-0.09378455579280853,
0.030151676386594772,
0.03847122564911842,
-0.026679379865527153,
0.04782364144921303,
0.3443032503128052,
-0.012266378849744797,
0.035144444555044174,
-0.10294672846794128,
0.02033337391912937,
-0.023207729682326317,
-0.019182909280061722,
-0.027221156284213066,
0.026869311928749084,
-0.0031949898693710566,
0.07215150445699692,
0.05694645643234253,
0.03747756406664848,
0.003972340375185013,
-0.04193836450576782,
0.0049268510192632675,
0.06193334236741066,
0.0016112640732899308,
-0.03949444741010666,
-0.017137138172984123,
0.08460066467523575,
-0.052413564175367355,
0.0032537993974983692,
0.046290282160043716,
0.015483500435948372,
0.03650534898042679,
0.06866218149662018,
-0.004865878261625767,
0.07842109352350235,
0.047990359365940094,
0.040937479585409164,
0.005993950646370649,
0.02610357105731964,
-0.03517173230648041,
0.019959600642323494,
0.00624639680609107,
-0.025278598070144653,
0.01863148808479309,
-0.010304175317287445,
-0.05709409713745117,
-0.03101455792784691,
-0.019153231754899025,
-0.07504250854253769,
-0.05929560586810112,
-0.05535319820046425,
-0.004017950035631657,
0.05978131294250488,
0.010641447268426418,
0.021047472953796387,
-0.008767449297010899,
-0.0068111480213701725,
0.023655276745557785,
0.06102711707353592,
-0.05605405941605568,
0.04173275828361511,
-0.005244162864983082,
0.022990968078374863,
-0.05939195677638054,
-0.01818127930164337,
0.031362708657979965,
-0.03802592307329178,
-0.04249251261353493,
0.004593775607645512,
-0.006044758018106222,
0.07098187506198883,
0.02939598262310028,
-0.033225614577531815,
-0.08754105120897293,
0.005298069212585688,
-0.06392788887023926,
0.029832957312464714,
0.025652961805462837,
0.018765948712825775,
-0.015676019713282585,
0.03922708332538605,
-0.012154348194599152,
-0.002403838559985161,
0.02451675571501255,
0.016915369778871536,
0.0001008554536383599,
0.02894003316760063,
0.050370633602142334,
-0.03192600980401039,
-0.07169275730848312,
0.05967811867594719,
0.017842652276158333,
-0.034345440566539764,
-0.07035589963197708,
0.010176201350986958,
-0.0033058850094676018,
0.06083707511425018,
0.02957984432578087,
0.03648830205202103,
-0.11791206896305084,
-0.017053311690688133,
-0.026765180751681328,
0.003205209970474243,
-0.020119445398449898,
0.04379928112030029,
-0.0028872720431536436,
-0.04375189542770386,
0.09972485154867172,
0.011940959841012955,
-0.011847148649394512,
0.006636489648371935,
-0.012445871718227863,
0.06246355548501015,
-0.005484496243298054,
0.026269035413861275,
-0.012437498196959496,
0.019079718738794327,
-0.035746946930885315,
-0.004516199696809053,
-0.11146266013383865,
-0.004833127837628126,
-0.014420650899410248,
0.02521587908267975,
0.035487398505210876,
-0.040826935321092606,
0.0652579665184021,
-0.05006289482116699,
-0.0043055773712694645,
-0.0663396418094635,
-0.09003124386072159,
-0.3090205788612366,
-0.012584568001329899,
0.05405107140541077,
-0.043818455189466476,
0.018864765763282776,
-0.06071554496884346,
0.00986192561686039,
0.026961063966155052,
0.02540871873497963,
-0.0235242061316967,
0.05639931559562683,
0.05821976438164711,
-0.034329816699028015,
-0.031502097845077515,
0.012367975898087025,
0.000009358370334666688,
0.030188148841261864,
-0.027272487059235573,
0.0067413984797894955,
0.025603877380490303,
-0.015614948235452175,
0.01717253401875496,
0.002391715534031391,
-0.02105557732284069,
0.04990154877305031,
-0.012595192529261112,
0.08528269827365875,
0.0006409783381968737,
0.01003396138548851,
-0.021572742611169815,
0.016906237229704857,
0.04087219759821892,
-0.029481956735253334,
-0.11479773372411728,
-0.03133544325828552,
-0.08527380228042603,
-0.04272942245006561,
0.02535000443458557,
0.025311458855867386,
0.02696739323437214,
-0.015757780522108078,
0.012722145766019821,
-0.009827563539147377,
-0.073508121073246,
-0.0400717556476593,
-0.006490112282335758,
0.0014963890425860882,
-0.007530075032263994,
0.026573246344923973,
-0.03382401540875435,
0.04003462940454483,
0.021160244941711426,
0.01995098777115345,
-0.0269088726490736,
0.005835539661347866,
-0.03130339831113815,
-0.06000920757651329,
-0.02566339448094368,
-0.03650176152586937,
0.024146609008312225,
-0.0007177719380706549,
-0.023098206147551537,
-0.04233487695455551,
0.037753187119960785,
0.02114223875105381,
0.046962909400463104,
0.0133042698726058,
0.0205570999532938,
0.0649266168475151,
-0.0010700681013986468,
0.03776168450713158,
0.04112926870584488,
-0.059665169566869736,
0.055165719240903854,
0.0301076490432024,
0.01697717234492302,
-0.038604237139225006,
-0.05333690345287323,
-0.04921122267842293,
0.05967244505882263,
0.031342945992946625,
-0.014213524758815765,
0.029669340699911118,
0.006252053193747997,
-0.0047728572972118855,
0.04822934418916702,
-0.01726330630481243,
0.025166373699903488,
-0.01027710735797882,
0.03847111761569977,
0.030971672385931015,
-0.07110531628131866,
0.016119031235575676,
-0.04190237820148468,
0.062488481402397156,
0.013206585310399532,
-0.2398039847612381,
-0.0008171428344212472,
-0.039539869874715805,
0.047848477959632874,
-0.03318282589316368,
0.005444536451250315,
0.03775940090417862,
-0.07530859857797623,
-0.02704564854502678,
0.07493766397237778,
0.03155310079455376,
-0.002382108010351658,
0.1315527707338333,
0.07776372134685516,
-0.020351635292172432,
0.022872669622302055,
0.04476003721356392,
0.02956988662481308,
0.04117162898182869,
-0.010488320142030716,
0.0012165153166279197,
-0.0823763832449913,
0.19141191244125366,
-0.03891390934586525,
-0.041913118213415146,
0.05394824966788292,
-0.04835300147533417,
0.015874916687607765,
0.0008613549289293587,
0.048785168677568436,
0.0025729441549628973,
0.022748932242393494,
0.07755729556083679,
0.014603865332901478,
0.002971093636006117,
0.0369502417743206,
-0.061387769877910614,
-0.008081771433353424,
-0.035768892616033554,
-0.00445186672732234,
-0.04003990814089775,
0.028290847316384315,
-0.06109479442238808,
-0.036241594702005386,
0.06559067964553833,
-0.0985712856054306,
-0.07551949471235275,
-0.0868474543094635,
0.02853206917643547,
-0.008774645626544952,
0.014857887290418148,
0.018148379400372505,
0.002836835104972124,
-0.007300929632037878,
-0.03204844519495964,
-0.02528485096991062,
-0.03227150812745094,
0.03407641872763634,
-0.018458018079400063,
-0.03476501628756523,
-0.05366739630699158,
0.012714443728327751,
-0.0018230171408504248,
0.12736868858337402,
-0.011314192786812782
] |
PMID:27219 | Poly(A) polymerases of rat liver nuclei. Purification and specificity. | Two poly(A) polymerases were isolated from rat liver nuclei and purified more than one thousand times by ion exchange chromatography on DEAE-Sephadex and phosphocellulose columns as well as affinity chromatography on a chromosomal RNA-Sepharose column. One of the two enzymes is bound to chromatin and uses as primer chromosomal RNA, while the second one is localized in the nucleoplasm and uses as primer poly(A) and hnRNA isolated from chromatin. The two enzymes seem to participate in the polyadenylation of chromosomal RNA in vitro, by a coupled mechanism. According to this mechanism, the chromatin bound enzyme adds 120-130 adenosine nucleotides to chromosomal RNA and consequently the nucleoplasmic enzyme completes the poly-adenylation by adding 80-90 more AMP units to the polyadenylated end of chromosomal RNA. | Poly(A) polymerases of rat liver nuclei. Purification and specificity. Two poly(A) polymerases were isolated from rat liver nuclei and purified more than one thousand times by ion exchange chromatography on DEAE-Sephadex and phosphocellulose columns as well as affinity chromatography on a chromosomal RNA-Sepharose column. One of the two enzymes is bound to chromatin and uses as primer chromosomal RNA, while the second one is localized in the nucleoplasm and uses as primer poly(A) and hnRNA isolated from chromatin. The two enzymes seem to participate in the polyadenylation of chromosomal RNA in vitro, by a coupled mechanism. According to this mechanism, the chromatin bound enzyme adds 120-130 adenosine nucleotides to chromosomal RNA and consequently the nucleoplasmic enzyme completes the poly-adenylation by adding 80-90 more AMP units to the polyadenylated end of chromosomal RNA. | [
-0.04941753298044205,
-0.028372453525662422,
0.004031652584671974,
-0.03550146147608757,
-0.01609691046178341,
0.0051433974876999855,
0.045328401029109955,
0.01849130541086197,
0.0022859456948935986,
0.03624723479151726,
0.01660887897014618,
-0.025454983115196228,
-0.04891938716173172,
-0.032596610486507416,
-0.046714916825294495,
0.008370422758162022,
-0.03940782696008682,
-0.03260922431945801,
-0.05458593741059303,
0.00938910897821188,
0.009117533452808857,
-0.03504147008061409,
0.0055084796622395515,
-0.01176363229751587,
-0.024322735145688057,
0.03514159470796585,
-0.0175301656126976,
-0.059722352772951126,
-0.047579530626535416,
-0.20954494178295135,
-0.017760103568434715,
-0.01654081977903843,
0.06190593168139458,
-0.054105013608932495,
0.016212502494454384,
-0.01806299202144146,
-0.02861350029706955,
-0.00547734135761857,
-0.015698062255978584,
0.06321043521165848,
0.026184149086475372,
0.015246872790157795,
-0.022970598191022873,
-0.008866251446306705,
0.04294523224234581,
0.0371711403131485,
-0.02283073402941227,
-0.025019221007823944,
0.050097111612558365,
-0.010811807587742805,
-0.012455230578780174,
0.053726475685834885,
-0.020225316286087036,
-0.0203072689473629,
-0.01087274495512247,
0.021898260340094566,
0.04404430091381073,
0.11655422300100327,
-0.015504597686231136,
0.03335198760032654,
-0.029411837458610535,
0.011807959526777267,
-0.12037976831197739,
0.08559321612119675,
0.05401588976383209,
-0.0007781941094435751,
0.00571477459743619,
-0.026803260669112206,
-0.0023578088730573654,
0.04449092596769333,
-0.06955043971538544,
0.04928755760192871,
0.028996586799621582,
0.048094164580106735,
-0.013876580633223057,
-0.012637630105018616,
-0.008201715536415577,
-0.06662110984325409,
0.06464192271232605,
0.0035499127116054296,
-0.0595400407910347,
-0.00914191734045744,
-0.005682656075805426,
-0.02488662302494049,
-0.057312704622745514,
0.08297321945428848,
-0.06231977418065071,
-0.014684086665511131,
-0.008621659129858017,
-0.03081461973488331,
0.0005319360643625259,
-0.009448136202991009,
0.020419640466570854,
-0.0072520519606769085,
-0.04338690638542175,
0.04296021908521652,
0.08940592408180237,
-0.01820441335439682,
0.08627379685640335,
0.3363579511642456,
-0.010482837446033955,
0.009646092541515827,
-0.05564085766673088,
-0.013712944462895393,
-0.0678042322397232,
0.013430103659629822,
-0.023686235770583153,
-0.008756794966757298,
-0.007463798858225346,
0.03823613375425339,
0.051881831139326096,
0.034950483590364456,
0.005729557480663061,
-0.012133997865021229,
0.026164516806602478,
-0.028568565845489502,
0.012334054335951805,
-0.0031421866733580828,
-0.030187485739588737,
0.04627055674791336,
-0.0544455461204052,
-0.05346481874585152,
0.022540288046002388,
-0.0028026183135807514,
0.04514914005994797,
0.032115112990140915,
-0.0010233672801405191,
0.07748964428901672,
0.07501328736543655,
0.07144296169281006,
0.1060071811079979,
0.038964949548244476,
-0.026906004175543785,
0.012800747528672218,
0.03665946424007416,
-0.04474601149559021,
0.029285622760653496,
-0.009757927618920803,
0.011521706357598305,
-0.022782349959015846,
0.026582472026348114,
-0.06864073127508163,
-0.05400341376662254,
-0.017406288534402847,
-0.03408156335353851,
0.06548382341861725,
-0.027520351111888885,
0.05291051045060158,
0.023752650246024132,
-0.03166988864541054,
-0.009903719648718834,
0.08611799031496048,
-0.06642120331525803,
0.023924170061945915,
0.007068960927426815,
0.02023988403379917,
-0.000765135744586587,
-0.07480292022228241,
0.006757472641766071,
0.025786271318793297,
-0.07368550449609756,
-0.024095719680190086,
0.004432475194334984,
0.04639596492052078,
0.03088466078042984,
-0.04026355221867561,
-0.019468586891889572,
0.011374644935131073,
0.021321002393960953,
-0.012034463696181774,
0.016292467713356018,
-0.029841523617506027,
0.0022210751194506884,
0.01960415206849575,
-0.08114349842071533,
-0.06123897433280945,
0.03734063357114792,
0.06400392204523087,
0.04415320232510567,
0.008190811611711979,
-0.03657389432191849,
-0.09183858335018158,
-0.04311316832900047,
0.08014565706253052,
0.013713669963181019,
-0.00812707282602787,
-0.06299428641796112,
0.019910896196961403,
0.033122241497039795,
0.022473320364952087,
0.0072458223439753056,
0.06405117362737656,
-0.08536278456449509,
0.03999059647321701,
-0.08721128851175308,
-0.03509847819805145,
0.007653239648789167,
0.05467301234602928,
-0.0169646255671978,
-0.025613335892558098,
0.11302373558282852,
0.033473167568445206,
0.04328484460711479,
0.09166496992111206,
-0.054598964750766754,
0.04100571200251579,
0.031085558235645294,
0.003128855722025037,
0.006083474960178137,
0.043188076466321945,
-0.041979193687438965,
-0.00818441342562437,
-0.05771151930093765,
-0.004854746628552675,
-0.08564978837966919,
0.004478884395211935,
0.05629558116197586,
-0.007142768707126379,
0.03927091881632805,
0.022227732464671135,
-0.01674242876470089,
-0.08246074616909027,
-0.01924835704267025,
-0.27625370025634766,
-0.0010806876234710217,
-0.029405999928712845,
-0.021838651970028877,
0.029374614357948303,
-0.014151628129184246,
-0.00016360316658392549,
0.017064599320292473,
-0.028653983026742935,
-0.044395506381988525,
-0.019801517948508263,
0.026990965008735657,
-0.010147999972105026,
-0.018218712881207466,
-0.044847272336483,
0.07956098765134811,
0.006266330368816853,
0.04891763627529144,
0.013921232894062996,
0.006315247621387243,
0.017682738602161407,
-0.021170469000935555,
-0.003876275848597288,
0.04166775941848755,
0.031390927731990814,
0.009977203793823719,
0.10425817221403122,
0.028289444744586945,
-0.016861341893672943,
0.0379248671233654,
0.047210484743118286,
0.050923902541399,
-0.05937759205698967,
-0.09822142869234085,
-0.019829049706459045,
-0.046664152294397354,
-0.07601145654916763,
-0.022478479892015457,
0.024033257737755775,
0.013341248035430908,
0.025301199406385422,
0.031635936349630356,
-0.02925330586731434,
-0.051802292466163635,
0.013749045319855213,
0.005201810505241156,
-0.0472557358443737,
0.01319247204810381,
0.032389573752880096,
-0.010755626484751701,
0.058825310319662094,
-0.04063492268323898,
-0.01720387302339077,
-0.03035803884267807,
-0.009740769863128662,
0.016392534598708153,
-0.031531061977148056,
0.03842650726437569,
-0.056268658488988876,
0.07778769731521606,
0.0023391295690089464,
-0.011821452528238297,
-0.018996937200427055,
0.03099636174738407,
0.020252885296940804,
0.02029305137693882,
-0.011232171207666397,
0.015928590670228004,
0.03395409137010574,
-0.006432028952986002,
0.0342937596142292,
0.10240103304386139,
-0.007710024248808622,
0.012577446177601814,
0.039225440472364426,
0.037469252943992615,
-0.080078125,
-0.06728275120258331,
-0.06610853970050812,
0.0003647879930213094,
0.059159956872463226,
-0.06620688736438751,
0.04984673112630844,
0.007565031759440899,
-0.04484156519174576,
0.03951866924762726,
0.07738158106803894,
0.02434150129556656,
0.020669419318437576,
0.00013725366443395615,
0.011474031955003738,
0.04398481175303459,
0.013312181457877159,
-0.0306293535977602,
0.03037351928651333,
0.006392354611307383,
-0.27153345942497253,
-0.030068933963775635,
-0.003271731548011303,
-0.002249595243483782,
-0.04188247397542,
0.059156838804483414,
0.03235140070319176,
-0.028358368203043938,
-0.051396630704402924,
-0.007101390045136213,
0.043670762330293655,
0.0880943089723587,
0.06333406269550323,
0.018651055172085762,
-0.07258743047714233,
0.05920121818780899,
0.0814487636089325,
0.010645056143403053,
0.008337407372891903,
-0.045601025223731995,
-0.031058087944984436,
-0.07540615648031235,
0.1132887676358223,
-0.03462532162666321,
-0.0018027658807113767,
-0.036355361342430115,
-0.004563901573419571,
0.016074305400252342,
-0.01970568671822548,
0.03136991336941719,
0.0013823548797518015,
0.04264155775308609,
0.06242970749735832,
-0.07909028977155685,
0.01140706054866314,
0.04685075581073761,
-0.05039992928504944,
0.06963872164487839,
-0.012312515638768673,
0.004305003210902214,
-0.03302141651511192,
-0.012110728770494461,
-0.10010579228401184,
-0.06348218768835068,
0.0016678161919116974,
-0.021416600793600082,
-0.06993652880191803,
-0.0414506196975708,
0.01743234135210514,
0.003698426764458418,
-0.020364588126540184,
-0.011318283155560493,
0.04264681413769722,
0.024588938802480698,
-0.022354090586304665,
-0.024449773132801056,
-0.050666626542806625,
-0.0017350259004160762,
0.025570400059223175,
-0.021448882296681404,
0.022023964673280716,
0.027201084420084953,
-0.00462291669100523,
0.14996309578418732,
0.011232349090278149
] |
PMID:27220 | Inhibition of ribonuclease. Efficacy of sodium dodecyl sulfate, diethyl pyrocarbonate, protein ase K and heparin using a sensitive ribonuclease assay. | The effectiveness of several commonly used inhibitors of ribonuclease (RNAase) has been studied using the removal of radio-labelled leucine from leucyl-tRNA as a sensitive assay for RNAase activity. The inhibitors were tested under a variety of conditions, varying the temperature, the pH, and the source of RNAase. When each inhibitor is udes separately in the presence of pancreatic RNAase, sodium dodecyl sulfate (SDS) is the most effective; but during long exposures to temperatures above 0 degrees C considerable amounts of RNA are still degraded. Combination of inhibitors are more effective in preserving RNA; with this assay, a combination of SDS with diethyl pyrocarbonate is the most effective. Proteinase K acts as an inhibitor when used in combination with SDS; however, it has RNAase activity when used by itself. Diethyl pyrocarbonate, when used at the high range of concentrations employed by others for RNAase inhibition, reacts with RNA changing its charge. However, when diethyl pyrocarbonate is used in smaller amounts the effects on RNA are minimal, and when used in combination with SDS it effectively inhibits RNAase. | Inhibition of ribonuclease. Efficacy of sodium dodecyl sulfate, diethyl pyrocarbonate, protein ase K and heparin using a sensitive ribonuclease assay. The effectiveness of several commonly used inhibitors of ribonuclease (RNAase) has been studied using the removal of radio-labelled leucine from leucyl-tRNA as a sensitive assay for RNAase activity. The inhibitors were tested under a variety of conditions, varying the temperature, the pH, and the source of RNAase. When each inhibitor is udes separately in the presence of pancreatic RNAase, sodium dodecyl sulfate (SDS) is the most effective; but during long exposures to temperatures above 0 degrees C considerable amounts of RNA are still degraded. Combination of inhibitors are more effective in preserving RNA; with this assay, a combination of SDS with diethyl pyrocarbonate is the most effective. Proteinase K acts as an inhibitor when used in combination with SDS; however, it has RNAase activity when used by itself. Diethyl pyrocarbonate, when used at the high range of concentrations employed by others for RNAase inhibition, reacts with RNA changing its charge. However, when diethyl pyrocarbonate is used in smaller amounts the effects on RNA are minimal, and when used in combination with SDS it effectively inhibits RNAase. | [
-0.08464674651622772,
0.013561772182583809,
-0.04508993774652481,
0.018284238874912262,
0.038925014436244965,
-0.014271113090217113,
0.05158212408423424,
0.03953930363059044,
-0.0554361566901207,
-0.03362845256924629,
0.007724682800471783,
-0.05667576566338539,
0.0212259404361248,
0.012797502800822258,
-0.017438841983675957,
0.008466020226478577,
0.007125144824385643,
-0.009913057088851929,
-0.007626123260706663,
0.01845410279929638,
-0.009085264056921005,
0.0830528661608696,
0.024161260575056076,
-0.01662350445985794,
0.013897269032895565,
0.01650339923799038,
-0.03880126029253006,
-0.059830743819475174,
-0.08153983950614929,
-0.20676922798156738,
0.010737438686192036,
-0.06421951949596405,
0.007132438942790031,
-0.08190164715051651,
-0.06887909770011902,
0.07130176573991776,
-0.017933808267116547,
-0.029327277094125748,
-0.038122378289699554,
0.005759940017014742,
0.024604471400380135,
0.023602329194545746,
0.021661004051566124,
-0.020567797124385834,
-0.00865720771253109,
0.042748864740133286,
-0.002563623245805502,
-0.021760225296020508,
0.07805904000997543,
0.04314001649618149,
-0.04520396515727043,
-0.012106533162295818,
0.02528025396168232,
-0.009236093610525131,
0.01535838469862938,
-0.0035471885930746794,
0.012411707080900669,
0.09580004215240479,
-0.05596119537949562,
-0.005031071603298187,
0.013946399092674255,
0.009596429765224457,
-0.13329344987869263,
0.08371265232563019,
0.04361958056688309,
0.002752428874373436,
0.04655284434556961,
-0.043891094624996185,
0.06744544208049774,
0.06587628275156021,
0.002786479890346527,
0.02954915538430214,
-0.013187732547521591,
0.09058724343776703,
0.004250327590852976,
0.01735573075711727,
0.0013446337543427944,
-0.013509906828403473,
0.02793615125119686,
-0.0008669963572174311,
-0.03462668135762215,
-0.0087269376963377,
0.020104344934225082,
0.024906037375330925,
0.0024397915694862604,
0.014074192382395267,
0.04116692394018173,
-0.041944123804569244,
0.023631930351257324,
-0.02260614186525345,
0.003127183299511671,
0.016843294724822044,
-0.010092957876622677,
-0.06463894248008728,
-0.05356018617749214,
0.04893287271261215,
0.05930863320827484,
0.0025955424644052982,
0.05563124269247055,
0.3366810083389282,
-0.014368142001330853,
-0.034839749336242676,
-0.15409886837005615,
-0.050858400762081146,
-0.018106648698449135,
-0.062478382140398026,
-0.007658046204596758,
-0.009906002320349216,
0.004758275579661131,
0.0464056059718132,
0.08229908347129822,
0.03939878195524216,
-0.031789544969797134,
-0.020356029272079468,
0.030540181323885918,
0.008960452862083912,
0.04706905037164688,
0.0122446957975626,
-0.10039515048265457,
0.05416834354400635,
-0.017882265150547028,
-0.023601174354553223,
0.02252688817679882,
-0.019787456840276718,
0.029954252764582634,
0.028181839734315872,
0.018990574404597282,
0.10182176530361176,
0.022488698363304138,
0.015294167213141918,
0.07072342932224274,
-0.004997192416340113,
0.008330306969583035,
-0.04699873551726341,
0.02472262643277645,
-0.05418295040726662,
0.03911823779344559,
0.03160328045487404,
0.025475118309259415,
0.027499020099639893,
0.003856747644022107,
-0.04857612028717995,
-0.02684123069047928,
-0.0394585020840168,
0.015430024825036526,
0.03343832492828369,
-0.0642039030790329,
0.030322439968585968,
-0.0275737214833498,
-0.01269255205988884,
0.04215346649289131,
0.015603678300976753,
-0.014460009522736073,
0.012036976404488087,
0.025068623945116997,
-0.00022087209799792618,
-0.00014095714141149074,
-0.04846084490418434,
0.03522871434688568,
-0.011681142263114452,
-0.11466346681118011,
-0.012523039244115353,
0.02647440880537033,
0.04174592345952988,
-0.053328048437833786,
0.002412137808278203,
0.02487054467201233,
0.0391690768301487,
0.017397211864590645,
-0.027863701805472374,
-0.013057857751846313,
-0.053875625133514404,
0.02142309583723545,
-0.09033310413360596,
-0.040902700275182724,
0.0027966899797320366,
0.06259802728891373,
0.03582628443837166,
0.04063505306839943,
-0.023997437208890915,
-0.03686583787202835,
0.004260696470737457,
-0.0411175899207592,
-0.00036458042450249195,
0.054151833057403564,
-0.06491547077894211,
-0.010862291790544987,
-0.0031346946489065886,
0.1052747368812561,
0.029288748279213905,
-0.03407812863588333,
-0.01872122287750244,
-0.07103107124567032,
0.044935937970876694,
-0.032753653824329376,
-0.0033683031797409058,
-0.017324097454547882,
0.03424084931612015,
-0.011482147499918938,
0.010646882466971874,
0.08138366043567657,
0.014242731966078281,
-0.0007427529781125486,
0.028236713260412216,
0.07510244846343994,
0.07214713096618652,
-0.015097071416676044,
0.011966218240559101,
0.010566233657300472,
-0.0262829028069973,
-0.030936839058995247,
0.05298362299799919,
-0.046276625245809555,
0.01093975268304348,
-0.0799856036901474,
0.04941560700535774,
-0.007760367356240749,
0.026156015694141388,
0.003816931741312146,
0.0019492718856781721,
0.0062804571352899075,
-0.06480292230844498,
-0.01693401299417019,
-0.31790095567703247,
-0.03664059564471245,
0.01962602697312832,
0.0011375157628208399,
0.07843349128961563,
-0.061480212956666946,
-0.022345148026943207,
0.012226163409650326,
-0.03659993037581444,
-0.037805356085300446,
0.0016043027862906456,
0.0323883518576622,
0.017623502761125565,
-0.01091166865080595,
0.032469213008880615,
0.024474190548062325,
0.005394747946411371,
-0.036841560155153275,
-0.012163766659796238,
0.01495795976370573,
0.042056430131196976,
-0.04893777519464493,
0.002761652460321784,
-0.025429129600524902,
0.09947255998849869,
0.018117522820830345,
0.08665870875120163,
-0.014599512331187725,
0.02522602491080761,
0.008960075676441193,
0.017050309106707573,
0.07070008665323257,
0.01621464639902115,
-0.1146748811006546,
-0.018449019640684128,
-0.0064738295041024685,
-0.009001027792692184,
-0.04564773663878441,
0.024657804518938065,
0.0056773764081299305,
0.026924414560198784,
0.04841066524386406,
-0.04393874481320381,
-0.08967717736959457,
-0.021097591146826744,
-0.020770691335201263,
0.0015192449791356921,
0.01367818284779787,
0.02088380977511406,
0.004027097020298243,
-0.00448162155225873,
0.049457475543022156,
0.05738646537065506,
0.011967968195676804,
0.010541866533458233,
-0.0047420659102499485,
-0.03731454908847809,
-0.020169289782643318,
0.008665471337735653,
-0.02516431175172329,
-0.013624634593725204,
-0.020602433010935783,
-0.019562222063541412,
0.03812214359641075,
-0.011509289033710957,
-0.018203290179371834,
-0.003735878271982074,
-0.04386071860790253,
0.06436784565448761,
0.05419916659593582,
0.0046226163394749165,
0.1028677448630333,
-0.041002050042152405,
-0.02923712320625782,
0.008302144706249237,
0.06040685251355171,
-0.03266244754195213,
-0.06346340477466583,
-0.057511430233716965,
-0.02179053984582424,
0.0025407159700989723,
-0.0074479058384895325,
0.01349756121635437,
-0.05324789509177208,
-0.04151381924748421,
0.014406003057956696,
0.05990946292877197,
0.004941064398735762,
0.02909744158387184,
-0.030270293354988098,
-0.014717873185873032,
-0.07797534018754959,
0.0008221340249292552,
-0.03405316174030304,
0.038345202803611755,
0.005555268842726946,
-0.25976598262786865,
-0.009899072349071503,
0.001212593517266214,
-0.011485427618026733,
-0.04322443902492523,
0.04415414482355118,
0.030423574149608612,
-0.08709278702735901,
-0.026145843788981438,
0.009220121428370476,
0.02588527463376522,
0.04881894588470459,
0.0009100001188926399,
0.05014266446232796,
-0.007719371933490038,
0.012775254435837269,
0.08530252426862717,
-0.021303405985236168,
-0.022629965096712112,
-0.06768554449081421,
0.01684725284576416,
-0.09297387301921844,
0.16541273891925812,
-0.022416507825255394,
0.012921089306473732,
0.044815342873334885,
-0.010350500233471394,
0.024467241019010544,
-0.06267081201076508,
0.018965642899274826,
0.015555464662611485,
0.01568465866148472,
0.05119788274168968,
-0.0013322430895641446,
0.024065347388386726,
0.11043389141559601,
-0.0029106177389621735,
0.021932581439614296,
-0.0018643102375790477,
0.0012295893393456936,
0.025602594017982483,
0.027116825804114342,
-0.08021728694438934,
-0.005513399839401245,
0.04894126206636429,
-0.05944813787937164,
-0.018779311329126358,
-0.05599072948098183,
-0.003702713642269373,
-0.00642807874828577,
0.02879839390516281,
-0.023472756147384644,
0.040026288479566574,
-0.03618060424923897,
0.02716783992946148,
-0.001766100525856018,
-0.014057238586246967,
0.005915478803217411,
-0.037923414260149,
-0.002021751832216978,
0.010431141592562199,
-0.033723607659339905,
-0.0005732837016694248,
0.11867847293615341,
0.05519683659076691
] |
PMID:27221 | Intramembraneous localization of rat liver microsomal hexose-6-phosphate dehydrogenase and membrane permeability to its substrates. | A method for purifying hexose-6-phosphate dehydrogenase (beta-D-glucose: NAD(P) -oxidoreductase, EC 1.1.1.47) from rat liver microsomes is described. The purified enzyme was shown to be homogeneous by sodium dodecyl sulfate (SDS)-polyacrylamide electrophoresis. It is shown that the enzyme is bound to the inner surface of microsomal membranes, and that glucose 6-phosphate, but not NADP, penetrates almost freely into the membranes at 37 degrees C. | Intramembraneous localization of rat liver microsomal hexose-6-phosphate dehydrogenase and membrane permeability to its substrates. A method for purifying hexose-6-phosphate dehydrogenase (beta-D-glucose: NAD(P) -oxidoreductase, EC 1.1.1.47) from rat liver microsomes is described. The purified enzyme was shown to be homogeneous by sodium dodecyl sulfate (SDS)-polyacrylamide electrophoresis. It is shown that the enzyme is bound to the inner surface of microsomal membranes, and that glucose 6-phosphate, but not NADP, penetrates almost freely into the membranes at 37 degrees C. | [
-0.019758736714720726,
0.018710240721702576,
0.011390919797122478,
-0.03712189570069313,
0.013013004325330257,
0.003993677441030741,
0.025325924158096313,
-0.007360711693763733,
-0.036162883043289185,
-0.014777084812521935,
-0.014102069661021233,
-0.08921357244253159,
-0.011685006320476532,
-0.005701692309230566,
-0.019869372248649597,
0.009181690402328968,
0.015818851068615913,
0.029700489714741707,
-0.04644707217812538,
-0.01579916663467884,
0.018826525658369064,
-0.06658175587654114,
-0.009529242292046547,
-0.012399339117109776,
0.019046498462557793,
0.03717975690960884,
0.04754853993654251,
0.021216673776507378,
-0.06315987557172775,
-0.19489744305610657,
-0.006696626543998718,
-0.002864339854568243,
0.0789501890540123,
-0.06842196732759476,
0.026823516935110092,
0.005558646749705076,
0.022366730496287346,
-0.0018742659594863653,
-0.004952821880578995,
0.03958120569586754,
0.08143383264541626,
-0.005501396022737026,
0.022251872345805168,
0.07599834352731705,
-0.01834532618522644,
-0.033338744193315506,
-0.055984482169151306,
-0.025025954470038414,
0.06736519187688828,
-0.02086721733212471,
0.03745783865451813,
0.01794147863984108,
0.030455421656370163,
0.01262007374316454,
0.07041468471288681,
-0.016997940838336945,
0.06768368184566498,
0.02348155900835991,
-0.01445379015058279,
-0.002217449713498354,
0.030717317014932632,
0.016948161646723747,
-0.14677797257900238,
0.06477191299200058,
0.07935387641191483,
-0.041277844458818436,
-0.030626192688941956,
-0.07179926335811615,
0.02402392588555813,
-0.0022550884168595076,
-0.03433447331190109,
0.02620600536465645,
-0.010474732145667076,
0.006513499189168215,
0.019064871594309807,
-0.03087623417377472,
0.04843848943710327,
-0.03439081460237503,
0.044937312602996826,
0.05394193157553673,
0.03182442858815193,
-0.030961424112319946,
0.015638025477528572,
0.01877455785870552,
-0.03864024952054024,
0.012988056056201458,
0.04294935613870621,
-0.05419335141777992,
0.027545161545276642,
0.031124316155910492,
-0.012370645999908447,
-0.03350622206926346,
-0.06530459970235825,
0.019813772290945053,
-0.11851217597723007,
0.011705872602760792,
0.03457532823085785,
0.035415805876255035,
0.03660249710083008,
0.34136155247688293,
-0.025221742689609528,
0.04100764915347099,
-0.045820217579603195,
-0.04881647229194641,
-0.050967197865247726,
-0.07377449423074722,
-0.021337997168302536,
0.05074278265237808,
-0.00945393554866314,
0.05272281542420387,
0.0857619047164917,
-0.020067112520337105,
0.01977570541203022,
-0.09372805058956146,
0.033264726400375366,
0.02293708361685276,
-0.006438649725168943,
-0.003515516174957156,
-0.04190271720290184,
0.08326499909162521,
-0.016158225014805794,
0.002033000346273184,
-0.00379630783572793,
0.009900839067995548,
0.10688581317663193,
0.01746213249862194,
-0.03703094273805618,
0.11196417361497879,
0.11842484772205353,
0.045100778341293335,
0.02352827414870262,
0.03429511934518814,
-0.02098899707198143,
-0.029050277546048164,
-0.0378158874809742,
0.03124873712658882,
0.060820601880550385,
-0.07514046132564545,
0.024521103128790855,
-0.07558826357126236,
-0.043109145015478134,
-0.01748555339872837,
-0.0329272523522377,
-0.048772621899843216,
-0.028498535975813866,
0.0365709625184536,
-0.006027170456945896,
0.026439838111400604,
-0.012194905430078506,
-0.05034750699996948,
0.0567108690738678,
0.0022176187485456467,
-0.015971330925822258,
0.026520192623138428,
0.0108357397839427,
0.04345454275608063,
-0.027708852663636208,
-0.08287595957517624,
0.04236645624041557,
-0.007867305539548397,
-0.0690791979432106,
-0.021163349971175194,
0.035148605704307556,
0.09698478877544403,
0.05186505615711212,
0.033292289823293686,
-0.057545747607946396,
0.006709099747240543,
0.02936144731938839,
-0.03609133139252663,
-0.013683454133570194,
-0.03433232754468918,
-0.03307408466935158,
-0.059627581387758255,
-0.029933467507362366,
0.015404453501105309,
0.06404080241918564,
0.06637246906757355,
0.04263104125857353,
-0.05484761297702789,
-0.004286950454115868,
-0.05363855138421059,
-0.05306728184223175,
0.029175808653235435,
0.07188184559345245,
0.011309227906167507,
0.0149975111708045,
0.05622711405158043,
0.022901184856891632,
0.04264945164322853,
-0.07129673659801483,
0.032082587480545044,
-0.09128043800592422,
-0.030459893867373466,
0.012794875539839268,
-0.019790925085544586,
0.0050085498951375484,
-0.007297709118574858,
-0.015809526666998863,
-0.013478660956025124,
0.1458769589662552,
0.01799619011580944,
0.005293270573019981,
0.035610828548669815,
-0.03222820162773132,
0.0013470664853230119,
0.027404336258769035,
0.017422165721654892,
0.03709882125258446,
0.06457512080669403,
-0.05875109136104584,
0.010765780694782734,
-0.03698461875319481,
0.016258109360933304,
-0.09167075157165527,
0.005238028708845377,
0.041616953909397125,
-0.026383422315120697,
0.03661210462450981,
0.009251715615391731,
0.0393318273127079,
-0.06413306295871735,
-0.01932440884411335,
-0.2906903922557831,
-0.054696086794137955,
-0.0014122269349172711,
-0.03713059052824974,
0.022119659930467606,
-0.060711730271577835,
0.018308939412236214,
-0.026620788499712944,
-0.025670401751995087,
-0.028110850602388382,
0.06022373214364052,
0.002750602550804615,
-0.046506524085998535,
-0.03177836164832115,
-0.034311793744564056,
0.026592353358864784,
0.02810347080230713,
-0.04697881639003754,
0.03633381053805351,
0.023110590875148773,
0.016778508201241493,
0.0033509423956274986,
0.016042206436395645,
0.03774571791291237,
0.08401676267385483,
-0.056684304028749466,
0.09289298951625824,
0.022326597943902016,
0.02780020795762539,
-0.0201774463057518,
0.08838222920894623,
0.026254070922732353,
-0.027757924050092697,
-0.09458471834659576,
-0.043709564954042435,
-0.06545088440179825,
-0.023740511387586594,
-0.08252836763858795,
0.04497072473168373,
-0.013395520858466625,
-0.03430706262588501,
0.008866977877914906,
-0.02437637932598591,
-0.03176315873861313,
-0.027651377022266388,
-0.04694955796003342,
-0.02997787483036518,
-0.03185762092471123,
0.022861627861857414,
0.000592157244682312,
0.03177662193775177,
-0.015115381218492985,
0.032446976751089096,
-0.07401437312364578,
0.016812419518828392,
-0.029191913083195686,
-0.0059286123141646385,
-0.014088232070207596,
-0.06486760079860687,
0.03318467363715172,
-0.07797147333621979,
-0.03074313886463642,
-0.06322157382965088,
0.006016218103468418,
0.004043399356305599,
-0.0008335296879522502,
-0.029726646840572357,
-0.024344289675354958,
0.02734951116144657,
-0.0037744720466434956,
0.008687819354236126,
0.08512454479932785,
-0.03527770936489105,
-0.004585986025631428,
0.015525740571320057,
0.0486021414399147,
0.05191737413406372,
0.011942136101424694,
-0.05011977627873421,
0.008124570362269878,
0.011960133910179138,
-0.04412928223609924,
0.10152360796928406,
-0.003649916499853134,
0.004316587932407856,
0.06583335995674133,
0.006560883019119501,
-0.004806926939636469,
0.04854344576597214,
-0.00967733096331358,
-0.012183437123894691,
-0.016531599685549736,
-0.05225071683526039,
-0.0458267480134964,
0.020191414281725883,
0.011898220516741276,
-0.26247355341911316,
0.040821876376867294,
-0.03398331254720688,
-0.010681889951229095,
-0.035863641649484634,
0.03466639667749405,
0.07489194720983505,
0.009420163929462433,
-0.03205015882849693,
0.03468037024140358,
0.019203802570700645,
0.03260476514697075,
0.07832642644643784,
0.05776689946651459,
0.0007271197391673923,
0.019476501271128654,
0.017268721014261246,
0.023368820548057556,
-0.007097275927662849,
-0.04080541059374809,
0.006646172143518925,
-0.013751654885709286,
0.11009012907743454,
-0.04105318337678909,
-0.03758526220917702,
0.034146007150411606,
0.025358613580465317,
0.03015151433646679,
0.0029466943815350533,
0.042705945670604706,
-0.040677934885025024,
0.012542061507701874,
0.06020573154091835,
-0.05457775294780731,
0.004263840150088072,
-0.006209603510797024,
-0.026659412309527397,
-0.005773579701781273,
0.0031469841487705708,
0.02747987024486065,
-0.008192147128283978,
-0.009913403540849686,
-0.05467700585722923,
-0.009910535998642445,
0.07802166044712067,
-0.03205655887722969,
-0.06473667174577713,
0.004999010358005762,
0.04434886574745178,
0.019276877865195274,
0.0388016551733017,
0.0058886767365038395,
0.028327737003564835,
0.022503795102238655,
0.0013143954565748572,
-0.05431567132472992,
0.003472700947895646,
-0.019157087430357933,
-0.03998974710702896,
-0.008856567554175854,
0.01996847614645958,
-0.00010754334653029218,
-0.011746605858206749,
0.09940441697835922,
0.012801330536603928
] |
PMID:27223 | Binding of m-nitrobenzeneboronic acid to the active site of subtilisin BPN. | m-Nitrobenzeneboronic acid as a possible transition-state analog for serine proteases was found to cause absorption spectral change from 250 nm 350 nm upon binding with subtilisin BPN' (EC 3.4.21.14) at pH 6.5. Similar difference spectral changes of m-nitrobenzeneboronic acid were also observed at alkaline pH or upon addition of N-methylimidazole at pH 6.5. A characteristic circular dichroism spectrum of m-nitrobenezeneboronic acid was induced upon binding with subtilisin BPN' not only at pH 6.5, but also at alkaline pH. Circular dichroism spectral titration confirmed the stoichiometry of 1 : 1 for the m-nitrobenzeneboronic acid - subtilisin complex. m-Nitrobenzeneboronic acid was shown to be useful as a reversible chromophoric probe for the catalytic site of serine proteases. | Binding of m-nitrobenzeneboronic acid to the active site of subtilisin BPN. m-Nitrobenzeneboronic acid as a possible transition-state analog for serine proteases was found to cause absorption spectral change from 250 nm 350 nm upon binding with subtilisin BPN' (EC 3.4.21.14) at pH 6.5. Similar difference spectral changes of m-nitrobenzeneboronic acid were also observed at alkaline pH or upon addition of N-methylimidazole at pH 6.5. A characteristic circular dichroism spectrum of m-nitrobenezeneboronic acid was induced upon binding with subtilisin BPN' not only at pH 6.5, but also at alkaline pH. Circular dichroism spectral titration confirmed the stoichiometry of 1 : 1 for the m-nitrobenzeneboronic acid - subtilisin complex. m-Nitrobenzeneboronic acid was shown to be useful as a reversible chromophoric probe for the catalytic site of serine proteases. | [
-0.08307375758886337,
0.025993967428803444,
-0.053919676691293716,
-0.02407783642411232,
0.018648412078619003,
-0.021007830277085304,
0.06897085905075073,
0.059875261038541794,
-0.007962132804095745,
0.03228346258401871,
0.022036047652363777,
-0.08305839449167252,
0.03363186493515968,
0.03843717277050018,
-0.018177658319473267,
-0.06133203208446503,
-0.004234307445585728,
-0.01047774963080883,
-0.04697620868682861,
-0.0006493484252132475,
-0.030897008255124092,
0.002512171631678939,
0.06376397609710693,
0.010734016075730324,
0.04206551983952522,
0.06681714951992035,
-0.012813162989914417,
0.05580122396349907,
-0.04861318692564964,
-0.22921866178512573,
0.03166492283344269,
-0.002760709496214986,
0.060545243322849274,
-0.008384468965232372,
0.009725718759000301,
-0.027994146570563316,
-0.03739546239376068,
-0.011539367027580738,
0.010416198521852493,
0.033278100192546844,
0.1112222671508789,
0.055619653314352036,
0.0009048428037203848,
-0.006978263612836599,
0.0011906326981261373,
0.015127196907997131,
0.013675564900040627,
0.03073161654174328,
0.05347464233636856,
0.007796107325702906,
-0.032641246914863586,
-0.014338228851556778,
0.02148071490228176,
0.023469896987080574,
0.04839668795466423,
0.026361685246229172,
-0.012359460815787315,
0.0014479293022304773,
-0.014584488235414028,
0.04885031655430794,
0.03473123908042908,
0.010017713531851768,
-0.14071746170520782,
0.08725792914628983,
0.07004790753126144,
0.0016007019439712167,
0.017248433083295822,
-0.07473309338092804,
0.017057010903954506,
0.06209246441721916,
-0.03620067983865738,
0.022791175171732903,
0.0009248823043890297,
-0.014400550164282322,
0.008186081424355507,
-0.008283499628305435,
-0.0020029544830322266,
-0.041495051234960556,
0.014542264863848686,
-0.01731277070939541,
-0.0058038546703755856,
0.007355011999607086,
0.04805780574679375,
-0.03653694689273834,
0.0176081545650959,
0.03780900314450264,
-0.033269014209508896,
-0.05030900612473488,
0.038058798760175705,
0.038004226982593536,
0.008544126525521278,
-0.0443865992128849,
0.042418137192726135,
-0.024097520858049393,
-0.048673249781131744,
0.07612255215644836,
0.015181492082774639,
-0.0195582527667284,
0.0628698468208313,
0.37018468976020813,
-0.01523968018591404,
0.014788033440709114,
-0.0953073501586914,
-0.05572448670864105,
-0.027798201888799667,
-0.03564220294356346,
-0.04555622488260269,
0.018911212682724,
0.02825949341058731,
0.039029091596603394,
0.037182215601205826,
-0.005069628823548555,
0.008547745645046234,
-0.10698115080595016,
0.01458398811519146,
-0.014185838401317596,
0.022317323833703995,
-0.014034857042133808,
-0.04056674614548683,
-0.04158901423215866,
-0.013730112463235855,
0.012070141732692719,
0.01812558062374592,
-0.00462231133133173,
0.03169342130422592,
0.04189614579081535,
-0.049802426248788834,
0.06859339773654938,
0.07474640756845474,
-0.044484641402959824,
-0.016687840223312378,
0.034480225294828415,
-0.0003003836318384856,
-0.01173598412424326,
-0.003259661141782999,
0.012214845046401024,
0.04499135911464691,
-0.04193943366408348,
0.025212468579411507,
0.0568716824054718,
-0.0649607852101326,
-0.03892474249005318,
-0.06317971646785736,
-0.022764692083001137,
-0.05085940659046173,
0.044601380825042725,
-0.0044038682244718075,
0.012663551606237888,
-0.005003760103136301,
0.03585323318839073,
-0.00032987864688038826,
0.05859319493174553,
-0.013956817798316479,
0.04459593445062637,
-0.0486447773873806,
0.05757958069443703,
0.021974751725792885,
-0.03777257353067398,
-0.00908611249178648,
0.012884722091257572,
-0.08711198717355728,
-0.027267491444945335,
-0.013379457406699657,
0.07936754822731018,
0.011381090618669987,
-0.04690641537308693,
-0.0038085647393018007,
-0.01246620062738657,
0.01206785999238491,
-0.04553644359111786,
-0.051580946892499924,
0.022021425887942314,
-0.013779262080788612,
-0.0013294917298480868,
0.024234483018517494,
0.03536144271492958,
0.0019062887877225876,
-0.0031699477694928646,
-0.03157790005207062,
0.012282284907996655,
0.04017151519656181,
-0.012835127301514149,
-0.004043562337756157,
0.019502103328704834,
0.015127801336348057,
-0.009573121555149555,
-0.07636117935180664,
0.03554205223917961,
0.011104854755103588,
0.034727346152067184,
-0.028465021401643753,
-0.0168145839124918,
-0.09677543491125107,
0.06796247512102127,
-0.04403681308031082,
-0.011555575765669346,
-0.019602764397859573,
-0.023053988814353943,
-0.00865623727440834,
-0.0333535261452198,
0.14772248268127441,
0.008281623013317585,
-0.020424166694283485,
0.040331918746232986,
0.030941924080252647,
0.011933553032577038,
0.029592569917440414,
0.040309369564056396,
0.030962718650698662,
-0.029249319806694984,
0.0035498279612511396,
0.040855132043361664,
-0.05900103971362114,
0.010518766939640045,
-0.058114971965551376,
-0.07386356592178345,
0.005035700276494026,
-0.0026327273808419704,
0.05862220376729965,
0.031077232211828232,
0.026979487389326096,
-0.019422460347414017,
-0.08679471909999847,
-0.26456189155578613,
0.011209884658455849,
0.00944097712635994,
-0.0026171139907091856,
0.03128529340028763,
-0.08280004560947418,
0.00581085029989481,
-0.02419576793909073,
0.010499495081603527,
-0.07792206853628159,
0.038062263280153275,
0.06490501761436462,
-0.02838459610939026,
-0.07314065098762512,
0.04093078896403313,
-0.0032253514509648085,
0.05707304924726486,
-0.05681310594081879,
0.04880454018712044,
-0.0044687241315841675,
-0.019462978467345238,
-0.04291217774152756,
-0.010120349004864693,
0.021102311089634895,
0.07122821360826492,
-0.024916313588619232,
0.09834993630647659,
-0.006660623010247946,
-0.013195597566664219,
-0.0109484251588583,
-0.036224354058504105,
0.0085569117218256,
-0.055795878171920776,
-0.09183866530656815,
-0.040572572499513626,
-0.04040440171957016,
0.0153604531660676,
-0.045823246240615845,
0.025454064831137657,
-0.04978899657726288,
0.034315478056669235,
0.0076920269057154655,
0.05856979265809059,
-0.049014557152986526,
-0.03288975730538368,
-0.028638089075684547,
0.020460177212953568,
0.024576153606176376,
0.016297487542033195,
-0.013612705282866955,
0.02622680552303791,
-0.03285562992095947,
0.008640251122415066,
-0.0016275966772809625,
-0.02360185608267784,
-0.01558751706033945,
0.005500438157469034,
0.04448327422142029,
0.004815555177628994,
-0.020205941051244736,
-0.03487051650881767,
0.010796657763421535,
-0.001557611278258264,
0.06904195994138718,
-0.010289061814546585,
-0.039104048162698746,
0.07383962720632553,
-0.05138082802295685,
0.09993233531713486,
0.021139388903975487,
0.01834106631577015,
0.09078358858823776,
-0.039948880672454834,
0.03687477111816406,
0.08139565587043762,
0.0611054003238678,
0.031866393983364105,
-0.021409733220934868,
-0.06892203539609909,
0.02073683962225914,
0.0047494410537183285,
-0.02479705587029457,
0.023969529196619987,
-0.027652719989418983,
-0.00854844693094492,
0.023830369114875793,
0.029414277523756027,
-0.02114267833530903,
-0.01652524434030056,
0.020939478650689125,
0.030082423239946365,
0.02449774742126465,
-0.0516483411192894,
-0.042225006967782974,
-0.012137225829064846,
0.01986764557659626,
-0.28034844994544983,
0.03929976746439934,
0.026369381695985794,
0.0331319160759449,
-0.019200513139367104,
0.027593480423092842,
0.0461789034307003,
-0.062169622629880905,
-0.07281218469142914,
0.029691698029637337,
-0.007948505692183971,
-0.00913237128406763,
0.05386251583695412,
0.03473420813679695,
0.027933338657021523,
0.0050310418009757996,
0.0235214252024889,
-0.012999200262129307,
0.015627562999725342,
-0.054530058056116104,
-0.02621522545814514,
-0.046719323843717575,
0.1816021353006363,
0.008178922347724438,
0.04732707515358925,
0.06326105445623398,
0.0062760720029473305,
0.030025415122509003,
0.009797061793506145,
0.05213204398751259,
-0.012112895026803017,
0.0020161306019872427,
0.08365834504365921,
-0.06617061793804169,
-0.00009773034253157675,
0.04155592992901802,
-0.03773494064807892,
-0.0250372476875782,
-0.03691542521119118,
-0.008083267137408257,
-0.00046449751243926585,
-0.041579779237508774,
-0.06029365584254265,
-0.07615197449922562,
0.05908029153943062,
-0.0407271534204483,
-0.09207592904567719,
0.021942127496004105,
-0.030406443402171135,
-0.020906595513224602,
-0.03443601727485657,
0.006337751168757677,
0.029243478551506996,
0.05178298056125641,
0.006397170480340719,
0.005025819409638643,
-0.0427836999297142,
-0.05601887032389641,
0.000703202560544014,
0.010877511464059353,
0.03627603128552437,
-0.010245968587696552,
-0.02992645837366581,
0.014441314153373241,
0.02427872084081173
] |
PMID:27224 | Excitation of indole-3-acetic acid (an auxin) in a linoleate-lipoxygenase system. | The weak luminescence that accompanies the linoleate-lipoxygenase reaction was greatly enhanced by the addition of indole analogues, and especially indole acetic acid. The main emitting species in the indole acetic acid-linoleate-lipoxygenase system was analysed spectrophotometrically in the visible region and ascribed to the transition of excited indole acetate in triplet state to its ground state. Such an excited indole acetate could be generated by transfer of energy from the excited CO2 and excited carbonyl (generated by the linoleate-lipoxygenase reaction) to indole acetate in the ground state, but not by cleavage of the dioxetane analog (positions 2 and 3 on the indole ring). | Excitation of indole-3-acetic acid (an auxin) in a linoleate-lipoxygenase system. The weak luminescence that accompanies the linoleate-lipoxygenase reaction was greatly enhanced by the addition of indole analogues, and especially indole acetic acid. The main emitting species in the indole acetic acid-linoleate-lipoxygenase system was analysed spectrophotometrically in the visible region and ascribed to the transition of excited indole acetate in triplet state to its ground state. Such an excited indole acetate could be generated by transfer of energy from the excited CO2 and excited carbonyl (generated by the linoleate-lipoxygenase reaction) to indole acetate in the ground state, but not by cleavage of the dioxetane analog (positions 2 and 3 on the indole ring). | [
-0.06923287361860275,
0.03215401619672775,
-0.022157274186611176,
0.07092445343732834,
0.010981966741383076,
0.020357506349682808,
0.03671479970216751,
0.0222818311303854,
-0.026384778320789337,
-0.025566143915057182,
0.06595983356237411,
-0.020031150430440903,
0.047366444021463394,
-0.043539535254240036,
0.0033870521001517773,
-0.05817017704248428,
0.036015115678310394,
-0.026484597474336624,
-0.08434069901704788,
-0.07251067459583282,
0.058517295867204666,
0.010251848958432674,
-0.001437433180399239,
0.005401731468737125,
0.01071444246917963,
0.028864627704024315,
0.02476196549832821,
0.027844656258821487,
-0.04385387524962425,
-0.1804295778274536,
0.01554773561656475,
0.04081273823976517,
0.03547694906592369,
-0.02260434255003929,
-0.0027038678526878357,
-0.0028873556293547153,
-0.04514765739440918,
-0.040526799857616425,
-0.05734345316886902,
0.027309440076351166,
0.0935625210404396,
-0.02517409808933735,
-0.03409776836633682,
0.001026532961986959,
-0.026815194636583328,
-0.07794564217329025,
-0.019648030400276184,
0.03389792516827583,
0.019611185416579247,
-0.05527060478925705,
0.02723345346748829,
0.01673540100455284,
0.034262146800756454,
0.03490365296602249,
0.05548996105790138,
-0.014225730672478676,
-0.006401404272764921,
0.01614212989807129,
0.04022711515426636,
-0.019833894446492195,
-0.00022577190247830003,
0.07849960774183273,
-0.1295423060655594,
0.0943002700805664,
0.004118761979043484,
-0.08187778294086456,
0.0045335725881159306,
-0.04294755682349205,
0.043239008635282516,
0.04790015518665314,
-0.05872223898768425,
0.05057911202311516,
-0.03380320593714714,
-0.03642585501074791,
0.001482184510678053,
0.00827974732965231,
0.0042124842293560505,
-0.01738603413105011,
-0.013230593875050545,
-0.03288339078426361,
0.0005876952782273293,
-0.039333276450634,
-0.008685454726219177,
0.0009603669750504196,
-0.028222765773534775,
-0.009906795807182789,
0.0014611982041969895,
-0.06201210618019104,
-0.0010482158977538347,
0.11282260715961456,
-0.019138218834996223,
-0.05914447829127312,
-0.02814463898539543,
-0.03327826410531998,
0.0020377645269036293,
0.04361175745725632,
-0.0028066139202564955,
-0.0261840783059597,
-0.014295036904513836,
0.3662807047367096,
-0.048988938331604004,
0.03491583094000816,
0.003779797116294503,
-0.06079935282468796,
-0.02941213548183441,
-0.06254678219556808,
-0.07784242928028107,
0.006742406636476517,
0.025854267179965973,
-0.013502685353159904,
0.08238184452056885,
-0.04711795225739479,
0.035003285855054855,
-0.03435062989592552,
-0.0240417942404747,
-0.02961507998406887,
0.06857538968324661,
-0.03175262361764908,
0.007473562378436327,
-0.014048057608306408,
0.018704019486904144,
0.037968266755342484,
0.004842143040150404,
0.02445111982524395,
-0.001588134211488068,
-0.015607685782015324,
-0.0034450641833245754,
0.05918493866920471,
0.05077384412288666,
0.006306254304945469,
0.028636137023568153,
0.050417136400938034,
-0.02567848190665245,
-0.0238929595798254,
-0.026808248832821846,
-0.02552824094891548,
0.0005673919804394245,
-0.008539068512618542,
0.02275797910988331,
-0.010023515671491623,
0.057468660175800323,
-0.014883843250572681,
0.028610294684767723,
-0.048912957310676575,
-0.03173678740859032,
0.07783415168523788,
0.023802345618605614,
0.01117008738219738,
-0.027515321969985962,
0.020291678607463837,
0.0008642906905151904,
-0.013130728155374527,
-0.05497751757502556,
0.06522109359502792,
0.031251322478055954,
0.07056781649589539,
0.03476160764694214,
0.0234617181122303,
0.01284705474972725,
0.029706040397286415,
-0.10023146122694016,
0.02447165548801422,
-0.06852897256612778,
0.027093522250652313,
-0.017883922904729843,
-0.057174425572156906,
-0.036754410713911057,
0.0327218696475029,
0.020954707637429237,
0.026351217180490494,
-0.008155258372426033,
0.006744725164026022,
0.0162337739020586,
-0.04218573868274689,
-0.04394471272826195,
0.02975570037961006,
0.05806576460599899,
0.016958219930529594,
0.004619743209332228,
0.04345683380961418,
0.05457211658358574,
-0.06393125653266907,
0.0027218484319746494,
0.023276612162590027,
0.00450855540111661,
-0.00977570004761219,
-0.07337843626737595,
0.032383643090724945,
-0.032190952450037,
0.02422475628554821,
-0.030523007735610008,
0.04761488735675812,
-0.08776551485061646,
0.0318489633500576,
-0.032138802111148834,
-0.07744418829679489,
-0.04728413000702858,
-0.016079014167189598,
0.025023289024829865,
0.0017693059053272009,
0.1417461484670639,
-0.06175636500120163,
0.023501630872488022,
0.045625951141119,
0.03562840446829796,
-0.0208570659160614,
0.04305075854063034,
0.028915708884596825,
0.020792605355381966,
-0.02339126355946064,
-0.0038831070996820927,
0.010288873687386513,
0.0018146525835618377,
-0.026955557987093925,
-0.12688040733337402,
0.0031294128857553005,
0.012261882424354553,
0.052187517285346985,
0.03132135421037674,
-0.023357490077614784,
0.025787094607949257,
-0.023882225155830383,
-0.043216168880462646,
-0.2946169972419739,
0.0004331106028985232,
-0.0045558977872133255,
-0.04821592941880226,
0.06733980774879456,
0.007204615976661444,
-0.00917903333902359,
-0.04651263728737831,
0.043910183012485504,
-0.05023353919386864,
-0.004018165171146393,
0.0805865228176117,
0.017601072788238525,
-0.04766753688454628,
0.031818363815546036,
0.010303772054612637,
-0.0027895085513591766,
-0.046969830989837646,
0.0480254590511322,
0.05619363114237785,
0.024049298837780952,
-0.052837952971458435,
-0.054340992122888565,
-0.022669196128845215,
0.1002923771739006,
-0.03370067477226257,
0.09998010098934174,
-0.0012242915108799934,
0.04146018996834755,
-0.01828063279390335,
-0.04006383568048477,
0.04981742054224014,
-0.02050359919667244,
-0.09105614572763443,
-0.008712779730558395,
0.019063472747802734,
0.0697229877114296,
-0.05642694979906082,
0.008645919151604176,
-0.05016858130693436,
0.022100122645497322,
-0.009450471960008144,
-0.035983338952064514,
-0.02783629298210144,
0.013520142994821072,
-0.02925308234989643,
0.034265030175447464,
0.010034946724772453,
-0.014298534020781517,
0.01688295602798462,
0.08314691483974457,
0.030852675437927246,
0.046120621263980865,
0.028902266174554825,
-0.018737435340881348,
-0.01753062754869461,
-0.02407694235444069,
0.03003714606165886,
-0.0063589951023459435,
-0.007579161785542965,
-0.032421208918094635,
-0.025896258652210236,
0.01248401589691639,
0.03534507378935814,
-0.008794848807156086,
0.03058706410229206,
0.006502931471914053,
-0.0043882690370082855,
0.0295961182564497,
-0.003278490388765931,
0.009447390213608742,
0.11461242288351059,
-0.07500099390745163,
-0.023582886904478073,
0.07123208045959473,
0.058383021503686905,
-0.01019522175192833,
-0.05454465001821518,
-0.08976209163665771,
0.04647541046142578,
-0.020315198227763176,
-0.038238901644945145,
0.018615862354636192,
-0.02488700859248638,
-0.05668304115533829,
-0.010726912878453732,
0.0036432472988963127,
-0.028441336005926132,
0.021360095590353012,
0.008402328938245773,
0.027818426489830017,
0.039326928555965424,
-0.0016226823208853602,
-0.019551295787096024,
0.03951156139373779,
0.00453556701540947,
-0.2611273229122162,
-0.02770915813744068,
0.027843622490763664,
0.032935719937086105,
-0.007373927626758814,
0.02732224017381668,
0.029858777299523354,
-0.009060205891728401,
-0.05404702201485634,
0.019073816016316414,
-0.025334496051073074,
-0.004527355078607798,
0.0016547066625207663,
0.05549411103129387,
0.016807328909635544,
-0.010203145444393158,
0.05383779853582382,
-0.04442868381738663,
0.012332943268120289,
-0.02389499358832836,
-0.05540668964385986,
-0.0007117738714441657,
0.15931198000907898,
0.05718943476676941,
-0.08355157822370529,
0.07444333285093307,
0.028322996571660042,
0.05475543439388275,
-0.10456962883472443,
0.01633203774690628,
0.007474286947399378,
0.010550646111369133,
0.025742754340171814,
-0.07916943728923798,
0.03417447581887245,
0.07798206806182861,
0.03318072110414505,
-0.057673074305057526,
-0.012481443583965302,
0.007490403018891811,
0.009641867130994797,
-0.03263645991683006,
-0.07389868050813675,
0.008227325044572353,
0.02158786915242672,
0.013971053063869476,
-0.02575347200036049,
-0.003459871280938387,
-0.019442012533545494,
0.011962605640292168,
-0.007067406550049782,
0.02427280694246292,
-0.027288366109132767,
0.0018065090989694,
0.02491677738726139,
-0.008813925087451935,
-0.03690677136182785,
0.007698637433350086,
0.002757182577624917,
0.0205528661608696,
0.02678133174777031,
-0.01490315143018961,
0.001890996703878045,
0.1027752235531807,
0.09339997172355652
] |
PMID:27225 | Lipid metabolism by The gall-bladder. II. The in vitro conversion of lysophosphatidylcholine to phosphatidylcholine. | Lysophosphatidylcholine acyltransferase, which catalyzes the acylation of lysophosphatidylcholine with fatty acid coenzyme A to form phosphatidylcholine, was assayed in gall-bladder mucosa. In guinea pig gall-bladder the activity parallels that of the microsomal enzyme, glucose-6-phosphatase with 3--4-fold enrichment of the activity in the microsomes. Studies with saturated and unsaturated substrates demonstrated highest activity when oleoyl coenzyme A and palmitoyl lysophosphatidylcholine were used and the lowest activity when palmitoyl coenzyme A and palmitoyl lysophosphatidylcholine were used. This activity was demonstrated in the dog, rabbit, cat, calf and human gall-bladder mucosa; however, a wide variation in the amount was observed. Lysophospholipase, which catalyzes the hydrolysis of lysophosphatidylcholine to glycerophosphorylcholine and fatty acid, was also demonstrated in gall-bladder mucosa. | Lipid metabolism by The gall-bladder. II. The in vitro conversion of lysophosphatidylcholine to phosphatidylcholine. Lysophosphatidylcholine acyltransferase, which catalyzes the acylation of lysophosphatidylcholine with fatty acid coenzyme A to form phosphatidylcholine, was assayed in gall-bladder mucosa. In guinea pig gall-bladder the activity parallels that of the microsomal enzyme, glucose-6-phosphatase with 3--4-fold enrichment of the activity in the microsomes. Studies with saturated and unsaturated substrates demonstrated highest activity when oleoyl coenzyme A and palmitoyl lysophosphatidylcholine were used and the lowest activity when palmitoyl coenzyme A and palmitoyl lysophosphatidylcholine were used. This activity was demonstrated in the dog, rabbit, cat, calf and human gall-bladder mucosa; however, a wide variation in the amount was observed. Lysophospholipase, which catalyzes the hydrolysis of lysophosphatidylcholine to glycerophosphorylcholine and fatty acid, was also demonstrated in gall-bladder mucosa. | [
-0.0071034859865903854,
-0.03637576475739479,
-0.018057409673929214,
0.06449709832668304,
-0.05673444643616676,
-0.06360101699829102,
0.040569793432950974,
0.034554217010736465,
-0.008448855951428413,
-0.04697617143392563,
0.016352619975805283,
-0.11597524583339691,
-0.03128431364893913,
-0.014775410294532776,
-0.060325704514980316,
-0.06453149765729904,
0.00839016493409872,
-0.019443299621343613,
-0.04891221970319748,
0.009506285190582275,
0.019299039617180824,
-0.006077773869037628,
0.004515873733907938,
-0.01860426552593708,
0.007952464744448662,
-0.006683923304080963,
-0.0018018886912614107,
-0.015893451869487762,
-0.0756039172410965,
-0.1667362004518509,
-0.020282745361328125,
-0.04647185653448105,
-0.00015745061682537198,
-0.032176606357097626,
0.04084901884198189,
-0.007772570941597223,
-0.01591838337481022,
-0.0010500304633751512,
-0.040143657475709915,
0.07192764431238174,
0.049469832330942154,
0.03783617168664932,
0.002174610970541835,
-0.0024114330299198627,
0.03345412760972977,
-0.02105037309229374,
-0.05480275675654411,
0.011032544076442719,
0.08294402807950974,
0.031358491629362106,
0.015754295513033867,
0.03149106353521347,
0.05160144343972206,
-0.005661144386976957,
0.000921879371162504,
-0.047621313482522964,
0.01693260483443737,
0.03893274813890457,
0.015253296121954918,
0.013299372978508472,
-0.026210669428110123,
0.033828213810920715,
-0.18442176282405853,
0.08679075539112091,
0.009091329760849476,
-0.04808637499809265,
0.007405573967844248,
-0.028230095282197,
0.032780200242996216,
0.05084211379289627,
-0.09145327657461166,
0.0009006086038425565,
-0.03346700221300125,
0.01832045614719391,
-0.01464602630585432,
0.02123310975730419,
0.04690299183130264,
0.032824039459228516,
0.02601769007742405,
0.0007984805852174759,
-0.005891859997063875,
-0.0037569140549749136,
-0.028298793360590935,
0.06425116956233978,
-0.023619484156370163,
0.04245048016309738,
0.012195978313684464,
-0.028960993513464928,
0.06296420842409134,
0.023173974826931953,
-0.02500922419130802,
-0.09093374758958817,
0.008429870940744877,
0.011775724589824677,
-0.0679357647895813,
0.0409838892519474,
0.03372686356306076,
0.0049461969174444675,
0.0004191117768641561,
0.3581170439720154,
-0.049769796431064606,
0.014018739573657513,
-0.03881704434752464,
-0.038823965936899185,
-0.028262753039598465,
-0.005059134215116501,
-0.01203582901507616,
0.0017445865087211132,
0.016435204073786736,
0.04160741716623306,
0.06222366541624069,
0.006247511133551598,
0.0042518991976976395,
-0.04296472296118736,
-0.04395148158073425,
-0.013586980290710926,
0.05010639503598213,
0.013245032168924809,
0.003372941166162491,
0.10341620445251465,
-0.006587367504835129,
-0.04760987311601639,
0.0059086913242936134,
-0.024053610861301422,
0.06355703622102737,
0.0560995452105999,
0.028666825965046883,
0.09603964537382126,
0.07028511166572571,
0.0432363860309124,
0.03433684632182121,
0.017118284478783607,
0.016094444319605827,
0.0343460887670517,
-0.035776287317276,
-0.006613611243665218,
0.06660256534814835,
0.03299320116639137,
-0.013432750478386879,
-0.04063013568520546,
-0.03466854616999626,
-0.03031027875840664,
-0.02531561255455017,
-0.04845653846859932,
-0.02060268074274063,
0.11859267950057983,
0.03314414620399475,
0.015674371272325516,
-0.05435849726200104,
0.024386102333664894,
0.06570747494697571,
0.022214017808437347,
-0.012068508192896843,
0.04787145182490349,
-0.06492216140031815,
0.005259309429675341,
0.01892753504216671,
0.052962254732847214,
0.029412614181637764,
-0.013423377647995949,
-0.07050064951181412,
-0.016209417954087257,
-0.05605632811784744,
0.06188416853547096,
0.013758093118667603,
-0.04197376221418381,
-0.009378896094858646,
0.013247022405266762,
-0.02059866674244404,
0.020259277895092964,
-0.012516357935965061,
-0.02053077705204487,
-0.018167437985539436,
-0.03504554182291031,
-0.046972569078207016,
0.029065150767564774,
0.01651744730770588,
0.018741225823760033,
-0.007594775408506393,
0.02122628316283226,
0.06719901412725449,
-0.0604286715388298,
-0.02587384544312954,
-0.008902707137167454,
0.006701361387968063,
0.001214245450682938,
-0.028342097997665405,
-0.01309868786484003,
-0.030226219445466995,
0.02969457395374775,
-0.0815635398030281,
-0.01196204498410225,
-0.09844716638326645,
-0.020365595817565918,
-0.03820953518152237,
-0.05805372819304466,
-0.04803628474473953,
0.020077208057045937,
-0.001916530542075634,
0.003588933264836669,
0.09970119595527649,
0.018226638436317444,
0.014007235877215862,
0.01800408773124218,
-0.037283092737197876,
0.004351681098341942,
-0.005479833576828241,
0.00493268109858036,
0.03232564777135849,
0.053094230592250824,
-0.0052263918332755566,
0.005094652064144611,
-0.029081549495458603,
-0.007397239096462727,
-0.08068627864122391,
0.048866499215364456,
-0.029333632439374924,
-0.031239952892065048,
0.04585827514529228,
-0.03427210822701454,
0.06148432940244675,
-0.04966626688838005,
-0.01394852064549923,
-0.3157855272293091,
-0.11734658479690552,
0.05583552271127701,
-0.007809446193277836,
0.08007670193910599,
-0.014432019554078579,
0.028594903647899628,
0.02686179056763649,
-0.017860928550362587,
-0.051646824926137924,
0.027230555191636086,
0.044088900089263916,
-0.04387911036610603,
0.043405402451753616,
-0.006098840851336718,
0.05796732380986214,
0.0011896637734025717,
-0.03222400322556496,
0.055126119405031204,
-0.014720141887664795,
0.02922510728240013,
-0.018916405737400055,
0.012909794226288795,
-0.02679905667901039,
0.08358526974916458,
0.01659689098596573,
0.10034282505512238,
0.023285040631890297,
-0.008064839988946915,
-0.020259229466319084,
0.04236332327127457,
0.030012601986527443,
0.019335389137268066,
-0.10559625923633575,
-0.01916690170764923,
0.004751844797283411,
-0.0073680332861840725,
-0.04365299642086029,
0.020272770896553993,
-0.010294473730027676,
-0.08001866191625595,
-0.012982137501239777,
-0.047281354665756226,
-0.04673289507627487,
-0.07593271136283875,
0.014725327491760254,
-0.036520376801490784,
-0.008470265194773674,
-0.020837202668190002,
-0.02002860978245735,
0.06658101826906204,
0.04127015545964241,
0.011039738543331623,
-0.04014046490192413,
-0.05077684670686722,
-0.0015207250835373998,
-0.012339143082499504,
0.02245897240936756,
0.02277427911758423,
0.0029033375903964043,
-0.05971064791083336,
0.03434676676988602,
0.010342872701585293,
0.021556811407208443,
0.0067590754479169846,
-0.013465171679854393,
-0.0020692674443125725,
0.012714019045233727,
0.020815296098589897,
0.017708711326122284,
0.03412714600563049,
0.06055159121751785,
-0.03800879046320915,
-0.025085357949137688,
0.028541235253214836,
0.05031771957874298,
-0.01667783595621586,
-0.022172557190060616,
-0.10560619831085205,
0.021191991865634918,
0.04476107284426689,
-0.03447742015123367,
0.08582144975662231,
-0.004062418360263109,
0.004458004143089056,
0.016797343268990517,
0.01836322247982025,
-0.012152264825999737,
0.030931532382965088,
0.013421404175460339,
-0.0019439572934061289,
0.03250357508659363,
-0.047661881893873215,
-0.06850651651620865,
-0.020599642768502235,
0.029947098344564438,
-0.2572176456451416,
0.032185908406972885,
-0.007250964641571045,
0.0196281298995018,
-0.0283905491232872,
0.010860081762075424,
0.05850818753242493,
0.0508219413459301,
-0.033838871866464615,
0.07836241275072098,
0.025265565142035484,
-0.00849730335175991,
0.07127301394939423,
0.07196591049432755,
0.0005956611130386591,
-0.0022315685637295246,
0.076210156083107,
0.05740411579608917,
0.045565828680992126,
0.009574429132044315,
0.003936926368623972,
-0.06377999484539032,
0.1342323273420334,
0.023610759526491165,
-0.0020996478851884604,
0.038777075707912445,
-0.04186398535966873,
-0.02751145511865616,
-0.017453473061323166,
0.002575887832790613,
-0.038590580224990845,
-0.008330908603966236,
0.060562554746866226,
-0.04554571583867073,
0.012328427284955978,
0.03634725883603096,
-0.006076021119952202,
0.009445101954042912,
-0.07420922815799713,
0.043872375041246414,
0.050708357244729996,
0.00934587325900793,
-0.06504683196544647,
-0.01929883100092411,
0.04739971086382866,
-0.067716583609581,
0.02568170614540577,
-0.06267037242650986,
-0.011027892120182514,
-0.06330149620771408,
0.02115686982870102,
-0.03980550542473793,
-0.0019304821034893394,
0.056829776614904404,
0.013184786774218082,
0.03064744547009468,
-0.01443864218890667,
0.032999612390995026,
-0.00395959010347724,
0.00610340153798461,
0.0018997055012732744,
-0.02662956528365612,
-0.035022955387830734,
0.11657754331827164,
0.05556216835975647
] |
PMID:27226 | Sites of direct and indirect halogenation of albumin. | The sites of radiohalogenation in proteins vary with the labeling method and the pH of the labeling reaciton. We have directly halogenated albumin with carrier-free radioiodide by three methods (pH range 2.2--9.3), and with carrier-free radiobromide by the chloroperoxidase method (pH range 2.2--4.6). Albumin was also indirectly halogenated by attaching a radioiodinated acylating agent, N-succinimidyl-3-(4-hydroxyphenyl) propionate (SHPP). The labeled proteins were proteolyzed enzymatically at neutral pH and the labeled amino acids produced were analyzed by liquid chromatography. Iodination at pH 7 yielded predominantly monoiodotyrosine, but at lower pH, fewer tyrosyl residues are labeled and a greater number of unstable sulfur-iodine bonds are formed at cysteinyl residues. Bromination with chloroperoxidase resulted in a high degree of labeling of cysteinyl residues at pH 2.8, the condition for optimum activity of this halogenating enzyme. Indirect halogenation with SHPP resulted in labeling of mid-chain lysyl, histidyl and tyrosyl residues. | Sites of direct and indirect halogenation of albumin. The sites of radiohalogenation in proteins vary with the labeling method and the pH of the labeling reaciton. We have directly halogenated albumin with carrier-free radioiodide by three methods (pH range 2.2--9.3), and with carrier-free radiobromide by the chloroperoxidase method (pH range 2.2--4.6). Albumin was also indirectly halogenated by attaching a radioiodinated acylating agent, N-succinimidyl-3-(4-hydroxyphenyl) propionate (SHPP). The labeled proteins were proteolyzed enzymatically at neutral pH and the labeled amino acids produced were analyzed by liquid chromatography. Iodination at pH 7 yielded predominantly monoiodotyrosine, but at lower pH, fewer tyrosyl residues are labeled and a greater number of unstable sulfur-iodine bonds are formed at cysteinyl residues. Bromination with chloroperoxidase resulted in a high degree of labeling of cysteinyl residues at pH 2.8, the condition for optimum activity of this halogenating enzyme. Indirect halogenation with SHPP resulted in labeling of mid-chain lysyl, histidyl and tyrosyl residues. | [
-0.06745875626802444,
-0.01202331017702818,
-0.06623617559671402,
-0.015634967014193535,
0.032359372824430466,
-0.010626842267811298,
0.03713282570242882,
0.01317255012691021,
-0.07441068440675735,
-0.0023278759326785803,
0.032482050359249115,
-0.07075062394142151,
-0.013320124708116055,
0.059063561260700226,
-0.008309844881296158,
-0.0385926179587841,
0.010320518165826797,
0.021273989230394363,
-0.038962870836257935,
-0.06679444760084152,
0.0296920258551836,
-0.026928137987852097,
0.04124097898602486,
-0.06061392277479172,
0.09879817068576813,
0.07743068039417267,
-0.018346410244703293,
0.038037944585084915,
-0.0474376417696476,
-0.1786089390516281,
-0.00656516058370471,
-0.047457437962293625,
0.0047298939898610115,
-0.045778948813676834,
-0.02876214124262333,
0.011619966477155685,
0.019963132217526436,
-0.037875693291425705,
-0.028042471036314964,
0.0007486456306651235,
0.10922017693519592,
0.033504944294691086,
-0.03083333559334278,
-0.05939680337905884,
0.05299472436308861,
0.035363100469112396,
-0.02390195243060589,
-0.004129698500037193,
0.03803940862417221,
0.05012589693069458,
0.025447027757763863,
0.021663224324584007,
-0.03343041241168976,
0.035510361194610596,
0.039980415254831314,
0.008456961251795292,
0.03208388388156891,
0.04556356370449066,
0.03289801627397537,
0.025990115478634834,
0.0053916503675282,
0.07431813329458237,
-0.14356258511543274,
0.04323770850896835,
0.05205981433391571,
0.03226389363408089,
-0.0031810628715902567,
-0.010687155649065971,
0.029683571308851242,
0.05393868684768677,
-0.052713777869939804,
-0.0024628108367323875,
-0.013309408910572529,
0.016269411891698837,
0.038627106696367264,
-0.0506126694381237,
-0.03069927915930748,
-0.0029269203078001738,
0.04851634427905083,
-0.03909516707062721,
-0.01252449955791235,
0.028065845370292664,
0.019678134471178055,
0.014902702532708645,
0.011412392370402813,
0.036493100225925446,
-0.009844438172876835,
-0.0036219428293406963,
0.013944386504590511,
0.05033036693930626,
-0.028746463358402252,
-0.07504305243492126,
0.014508198015391827,
-0.00885859876871109,
-0.03545066714286804,
0.06358376145362854,
-0.0022177628707140684,
-0.017444202676415443,
-0.028049085289239883,
0.37856513261795044,
-0.014130165800452232,
0.05457191541790962,
-0.014009982347488403,
0.039098650217056274,
-0.04486686736345291,
-0.06404238939285278,
-0.037127695977687836,
-0.0310138501226902,
-0.05472354590892792,
0.0011702013434842229,
0.019291983917355537,
0.016284873709082603,
0.01914508081972599,
-0.05311167240142822,
-0.007516576908528805,
0.05003423988819122,
0.018387043848633766,
0.009062656201422215,
-0.009030226618051529,
0.015498143620789051,
-0.009949053637683392,
-0.016027502715587616,
0.052665431052446365,
0.017068982124328613,
0.05662187933921814,
0.017105478793382645,
0.04429076611995697,
0.05776902288198471,
0.05944138392806053,
0.057658061385154724,
0.021626003086566925,
0.025363298133015633,
-0.05393305420875549,
0.05732261389493942,
0.03915421664714813,
-0.006298702675849199,
0.005422147456556559,
-0.055499982088804245,
0.014511903747916222,
0.01367896143347025,
-0.02144698053598404,
-0.04985692352056503,
-0.06367193907499313,
-0.04721967503428459,
-0.05637068673968315,
0.08585087209939957,
-0.012960625812411308,
0.04398686811327934,
-0.03414882346987724,
-0.004646113142371178,
-0.004786195233464241,
0.02037947252392769,
-0.026493653655052185,
0.04936843737959862,
-0.03580458462238312,
0.004612472839653492,
0.004248959477990866,
-0.030005477368831635,
0.024968570098280907,
0.005032251123338938,
-0.07654479891061783,
-0.003639973234385252,
0.02683505229651928,
0.09363232553005219,
0.02068108506500721,
-0.008813412860035896,
-0.004632207099348307,
0.026290608569979668,
0.06102728843688965,
0.04681508615612984,
-0.0319734662771225,
0.0626162737607956,
0.025232894346117973,
-0.05929052084684372,
-0.051281288266181946,
0.07712092250585556,
0.017927274107933044,
0.02358846925199032,
-0.001927644363604486,
0.00371933844871819,
-0.044445306062698364,
-0.03136221319437027,
-0.01365637592971325,
0.01567307859659195,
0.045366737991571426,
-0.06570083647966385,
-0.024919528514146805,
0.022073786705732346,
-0.008109780959784985,
0.07789835333824158,
-0.02694830484688282,
0.031055301427841187,
-0.047418758273124695,
-0.007810397073626518,
-0.02992103062570095,
-0.04855653643608093,
-0.030776914209127426,
0.037988774478435516,
0.03225813806056976,
0.0023005923721939325,
0.11592607945203781,
0.004477011971175671,
-0.03877982869744301,
0.04668146371841431,
0.042547013610601425,
0.013964572921395302,
0.014760657213628292,
-0.013941563665866852,
0.048140883445739746,
-0.0026155137456953526,
-0.0330653116106987,
-0.004576665349304676,
-0.053081586956977844,
0.014737201854586601,
-0.04978432506322861,
0.003175070509314537,
0.031174184754490852,
0.010389626026153564,
0.041487596929073334,
-0.03860257938504219,
-0.013701584190130234,
-0.08380410075187683,
-0.07533712685108185,
-0.3100570738315582,
-0.0008457903168164194,
-0.017112957313656807,
-0.053373441100120544,
0.036083001643419266,
-0.0960911363363266,
-0.01833464577794075,
0.013441363349556923,
-0.01357896439731121,
-0.0507890023291111,
0.029919514432549477,
-0.0004831928526982665,
-0.03311125189065933,
-0.0978112518787384,
0.0025219195522367954,
0.010290617123246193,
0.06435354799032211,
-0.032607562839984894,
0.06917036324739456,
-0.015266835689544678,
0.04366655275225639,
0.000882276683114469,
-0.017592258751392365,
0.022120444104075432,
0.06210150197148323,
-0.002914685755968094,
0.13238893449306488,
0.021511217579245567,
-0.005446642637252808,
0.048470139503479004,
-0.005482145119458437,
0.0013672729255631566,
-0.045832108706235886,
-0.08016149699687958,
-0.080095574259758,
-0.03272607550024986,
-0.0035468090791255236,
-0.05627507343888283,
-0.0047077699564397335,
-0.01956561580300331,
-0.050288911908864975,
0.03454292193055153,
-0.014289777725934982,
-0.05659962072968483,
0.004291679710149765,
-0.00647061737254262,
0.029900331050157547,
0.018827753141522408,
0.025818347930908203,
-0.001253154594451189,
0.06079290062189102,
0.0021840245462954044,
0.034043069928884506,
-0.025926686823368073,
-0.03157686069607735,
-0.019943730905652046,
-0.003859866876155138,
-0.012728817760944366,
-0.05037209764122963,
0.003314513247460127,
-0.009554220363497734,
-0.04914945363998413,
-0.01734817586839199,
0.04679479822516441,
0.008195163682103157,
-0.010529543273150921,
-0.013185533694922924,
-0.00124684430193156,
0.01971813477575779,
0.00852657575160265,
-0.0074129849672317505,
0.1193632110953331,
-0.06396979093551636,
0.012542269192636013,
0.03445277363061905,
0.053912848234176636,
-0.08209379762411118,
-0.0447588786482811,
-0.07503996044397354,
0.023581091314554214,
0.004383486229926348,
-0.015881473198533058,
0.07592784613370895,
0.013326834887266159,
0.004771272651851177,
0.04067579284310341,
0.013725795783102512,
0.0006532739498652518,
-0.02314942702651024,
0.051539622247219086,
0.010028649121522903,
0.006440087221562862,
-0.06152873858809471,
-0.04433220252394676,
0.05870587006211281,
0.014822280034422874,
-0.26819345355033875,
0.008322942070662975,
0.03365574777126312,
0.0341319739818573,
-0.046627722680568695,
0.014634225517511368,
0.04126705601811409,
-0.010999016463756561,
-0.026170486584305763,
0.052238546311855316,
-0.03303347900509834,
-0.016170794144272804,
0.036802276968955994,
0.005483096465468407,
-0.004719527903944254,
-0.0076590655371546745,
0.00009884274913929403,
-0.05264465883374214,
0.019368216395378113,
-0.010025963187217712,
0.004205936565995216,
-0.05606790632009506,
0.1436343640089035,
0.044658560305833817,
-0.042263999581336975,
0.052822090685367584,
0.0032077375799417496,
0.03554524481296539,
-0.02088778279721737,
0.05331650376319885,
0.020165910944342613,
0.0017606135224923491,
0.04981269687414169,
-0.01851050741970539,
0.06231314688920975,
0.02908743917942047,
0.027438165619969368,
-0.0171686839312315,
-0.09108148515224457,
0.022281508892774582,
-0.040095631033182144,
0.013692582957446575,
-0.08998148143291473,
-0.05096575245261192,
0.07265709340572357,
-0.0730084478855133,
0.011320381425321102,
-0.03711680695414543,
0.031136242672801018,
-0.023767046630382538,
-0.027353473007678986,
0.021624501794576645,
0.0024535178672522306,
0.010687111876904964,
0.004267069976776838,
-0.02991744875907898,
-0.012124850414693356,
-0.01623406819999218,
-0.07545351982116699,
0.016587767750024796,
0.060309458523988724,
-0.023242682218551636,
-0.01868147775530815,
0.08280427008867264,
0.036549318581819534
] |
PMID:27227 | Characteristics and conversion of high molecular weight forms of renin in plasma and their incomplete activation by the current acid treatment. | Two distinctly different high molecular weight forms of renin are present in mouse plasma in addition to the well-recognized active 40 000 dalton form. The biggest form has a molecular weight of about 800 000, and is stable in 4 M urea, but can be converted to the active 40 000 dalton form, by storage, exposure to acid and limited proteolysis. The 70 000 dalton form can be activated by acid and limited proteolysis. However, the 70 000 dalton form does not change molecular weight with activation. By measuring renin, not only by its enzymatic activity, but also by the direct radioimmunoassay for the renin molecule, which measures enzymatically active as well as inactive renin, it was found that both forms were activated but neither of them completely. The validity of the currently used term "total" renin as the enzymatic renin activity after acid activation, is, therefore, questionable. The quantitative significance of this must await methods which can ensure complete conversion or activation of the high molecular weight forms of renin in plasma. | Characteristics and conversion of high molecular weight forms of renin in plasma and their incomplete activation by the current acid treatment. Two distinctly different high molecular weight forms of renin are present in mouse plasma in addition to the well-recognized active 40 000 dalton form. The biggest form has a molecular weight of about 800 000, and is stable in 4 M urea, but can be converted to the active 40 000 dalton form, by storage, exposure to acid and limited proteolysis. The 70 000 dalton form can be activated by acid and limited proteolysis. However, the 70 000 dalton form does not change molecular weight with activation. By measuring renin, not only by its enzymatic activity, but also by the direct radioimmunoassay for the renin molecule, which measures enzymatically active as well as inactive renin, it was found that both forms were activated but neither of them completely. The validity of the currently used term "total" renin as the enzymatic renin activity after acid activation, is, therefore, questionable. The quantitative significance of this must await methods which can ensure complete conversion or activation of the high molecular weight forms of renin in plasma. | [
-0.1036095917224884,
-0.03208186477422714,
0.011831832118332386,
0.035518892109394073,
-0.05751384049654007,
-0.013805455528199673,
0.05188102275133133,
0.06058456003665924,
-0.026689579710364342,
-0.00012694379256572574,
-0.0032129697501659393,
-0.05449199303984642,
-0.00826253741979599,
0.03393055498600006,
-0.013556656427681446,
-0.04363878071308136,
0.02373773232102394,
-0.008637819439172745,
-0.08703538775444031,
-0.021483290940523148,
0.04247654229402542,
-0.011848495341837406,
0.09955258667469025,
0.03343242406845093,
0.05927102267742157,
0.009365055710077286,
-0.01605592854321003,
0.03943859040737152,
-0.05007264390587807,
-0.21907472610473633,
0.04865270480513573,
-0.005909724161028862,
0.026247819885611534,
-0.018136780709028244,
0.029642172157764435,
-0.01999102160334587,
-0.035925257951021194,
-0.01558942161500454,
-0.02751210145652294,
0.007878967560827732,
0.07942795008420944,
-0.014924006536602974,
0.007706834468990564,
0.028221122920513153,
0.03830956295132637,
0.02459949441254139,
-0.052400633692741394,
0.021145211532711983,
0.11707143485546112,
0.034109849482774734,
0.0205910075455904,
0.03342857584357262,
0.018125411123037338,
0.05803706496953964,
0.05059967190027237,
0.009676647372543812,
0.0035429392009973526,
-0.00020852334273513407,
-0.009236244484782219,
-0.0030607024673372507,
0.017677364870905876,
0.025027573108673096,
-0.1275639683008194,
0.05821622535586357,
0.040511354804039,
-0.001704299938865006,
-0.037687912583351135,
-0.07518191635608673,
0.01607891544699669,
0.030539410188794136,
-0.04293736070394516,
0.005694180261343718,
-0.04389165714383125,
0.011719140224158764,
-0.022683314979076385,
-0.0301953237503767,
-0.00837205071002245,
0.06441887468099594,
0.04795432090759277,
0.007156379520893097,
0.02393554523587227,
-0.023301443085074425,
0.04938841238617897,
-0.009331539273262024,
-0.033722296357154846,
-0.014762156642973423,
0.03630048409104347,
0.007944179698824883,
0.020723598077893257,
-0.025308582931756973,
-0.06362583488225937,
-0.009432844817638397,
0.003981713205575943,
-0.00525473291054368,
-0.0038686238694936037,
0.013097668066620827,
0.06218424811959267,
-0.02083057351410389,
-0.010406101122498512,
0.35367143154144287,
-0.0040963334031403065,
0.006907707545906305,
-0.08612274378538132,
0.0015027057379484177,
0.01717429608106613,
-0.01065259799361229,
-0.012073378078639507,
0.03239581733942032,
-0.0016999375075101852,
0.03869369998574257,
-0.018345924094319344,
0.016147658228874207,
0.007418283727020025,
-0.05780654773116112,
-0.030777808278799057,
0.000285944901406765,
0.024749355390667915,
0.0008114429074339569,
-0.013335902243852615,
0.08523715287446976,
0.022640718147158623,
0.0008592940866947174,
0.028788698837161064,
-0.005492638796567917,
0.006199072115123272,
0.024990711361169815,
0.012266088277101517,
0.040916942059993744,
0.05280783399939537,
0.006627749186009169,
0.062110401690006256,
0.009748307056725025,
-0.07996340841054916,
-0.020467620342969894,
0.02284887619316578,
-0.03941282629966736,
0.08749911189079285,
-0.02787203900516033,
0.05992596223950386,
0.009388824924826622,
-0.018875233829021454,
-0.006522688549011946,
-0.03316066041588783,
-0.07121437788009644,
-0.058222830295562744,
0.12523162364959717,
0.013390962034463882,
0.014478329569101334,
-0.02798202633857727,
0.022146573290228844,
0.018968375399708748,
0.08764482289552689,
-0.018518265336751938,
0.01335079688578844,
-0.006014036945998669,
0.06576525419950485,
0.005991853773593903,
0.012222378514707088,
0.015350308269262314,
-0.006696561351418495,
-0.032414887100458145,
-0.00396742345765233,
-0.03431623429059982,
0.028124094009399414,
0.09382293373346329,
0.012523012235760689,
-0.035758186131715775,
0.017457595095038414,
0.05665074661374092,
-0.001663523493334651,
-0.04035744443535805,
-0.009889766573905945,
-0.04077749326825142,
-0.0024592329282313585,
0.02103026770055294,
-0.005196459591388702,
-0.008995421230793,
-0.004734396934509277,
-0.00878906436264515,
-0.045403026044368744,
0.01509262714534998,
0.010578148998320103,
0.02096228115260601,
0.02303045429289341,
-0.004515829961746931,
-0.036910105496644974,
-0.04860200732946396,
-0.0005997241823934019,
0.01749519445002079,
0.11246200650930405,
0.0012511303648352623,
0.01692592166364193,
-0.047551609575748444,
-0.003960829693824053,
-0.023690881207585335,
-0.028077565133571625,
-0.06049235537648201,
-0.03306097909808159,
-0.04979401081800461,
0.02771979756653309,
0.05552447587251663,
-0.014883819967508316,
0.004818748217076063,
0.024538537487387657,
0.0298265740275383,
0.044303204864263535,
0.010811376385390759,
0.03376798704266548,
0.037009380757808685,
0.02536575309932232,
-0.03090151958167553,
0.030210884287953377,
-0.023682735860347748,
-0.021554023027420044,
-0.11528672277927399,
-0.038427118211984634,
-0.02748434618115425,
0.017836855724453926,
0.04300495982170105,
-0.010520746000111103,
0.028233874589204788,
-0.07289307564496994,
-0.02733570896089077,
-0.33514341711997986,
-0.013981986790895462,
0.027906622737646103,
-0.020600123330950737,
0.12799513339996338,
-0.02946590445935726,
0.014618774875998497,
-0.05428369343280792,
-0.020650355145335197,
-0.028633059933781624,
0.05096995830535889,
0.04050306975841522,
0.006883225869387388,
-0.013500281609594822,
0.0027694215532392263,
0.047987326979637146,
-0.010522873140871525,
-0.03309660404920578,
0.028170911595225334,
0.00655680987983942,
-0.0018287263810634613,
-0.029269801452755928,
0.00979253277182579,
0.06992019712924957,
0.04126330465078354,
0.02128041535615921,
0.10833205282688141,
-0.03826429322361946,
-0.018489539623260498,
0.024246662855148315,
-0.008083649910986423,
-0.014094923622906208,
-0.057025786489248276,
-0.05724668130278587,
-0.007815252989530563,
-0.06434213370084763,
-0.03611237183213234,
-0.012533102184534073,
-0.02706058882176876,
-0.010295852087438107,
-0.009849904105067253,
-0.029943078756332397,
0.004479168448597193,
-0.06757590919733047,
0.0049713547341525555,
0.05627390742301941,
0.04679335281252861,
0.07192430645227432,
-0.00477084144949913,
0.008038902655243874,
0.027382902801036835,
0.023258371278643608,
-0.027561187744140625,
-0.03177119418978691,
0.010090875439345837,
-0.012806694954633713,
-0.09336325526237488,
0.003267928957939148,
-0.044103071093559265,
-0.02149355225265026,
-0.020306257531046867,
0.009943057782948017,
-0.030675077810883522,
0.005150973796844482,
-0.04741736501455307,
0.010562095791101456,
-0.057961151003837585,
0.009201103821396828,
0.05133623629808426,
-0.010491841472685337,
-0.0007339082076214254,
0.08540870249271393,
-0.0287688709795475,
0.041757937520742416,
0.07922174036502838,
0.037379227578639984,
-0.032385069876909256,
-0.03376920521259308,
-0.02210403047502041,
0.030555669218301773,
-0.030836038291454315,
-0.0886809229850769,
0.03613943234086037,
-0.006600134074687958,
0.06654320657253265,
0.049688227474689484,
0.04776382818818092,
-0.009145233780145645,
0.043964486569166183,
0.04767247289419174,
0.019726522266864777,
0.012090475298464298,
-0.031436871737241745,
-0.032867737114429474,
-0.03476094827055931,
0.014475842006504536,
-0.29601964354515076,
-0.00040192148298956454,
0.03929230198264122,
0.04996873438358307,
-0.015041209757328033,
-0.031324174255132675,
0.02104591205716133,
-0.031830284744501114,
-0.07237900048494339,
0.029534051194787025,
-0.0136721758171916,
0.03852571174502373,
0.056378286331892014,
0.04425225034356117,
-0.029059167951345444,
-0.05506889522075653,
0.015710050240159035,
-0.018037892878055573,
0.0030599043238908052,
-0.027469972148537636,
-0.05194568634033203,
-0.04383343458175659,
0.13559061288833618,
-0.0012591410195454955,
-0.010264488868415356,
0.03680465370416641,
-0.055979494005441666,
0.026007268577814102,
-0.0940859317779541,
-0.022661644965410233,
-0.020424213260412216,
0.0218578539788723,
-0.012780961580574512,
-0.06158975511789322,
0.02887887880206108,
0.059958506375551224,
-0.01943107880651951,
0.0085822818800807,
0.00982301402837038,
0.019806623458862305,
-0.014032205566763878,
-0.022978398948907852,
-0.11318657547235489,
-0.02996184304356575,
0.07186222821474075,
-0.10489920526742935,
-0.01851063221693039,
-0.012782699428498745,
0.0062606181018054485,
-0.020824121311306953,
-0.04760326072573662,
0.00359756825491786,
0.036226291209459305,
-0.011806771159172058,
0.02585195191204548,
0.018016785383224487,
0.035754360258579254,
-0.02370179444551468,
0.003387466771528125,
0.007828685455024242,
0.01123883668333292,
0.07868845015764236,
-0.03522496297955513,
0.06428566575050354,
0.01900465413928032
] |
PMID:27228 | Effect of pH on bovine liver catalase as determined by electron paramagnetic resonance. | Two major rhombic high-spin ferric heme signals are observed during the pH titration of bovine liver catalase. The less rhombic signal if dominant above pH 6.0 and the more rhombic signal below pH 6.0. Ethanol in high concentration enhances the relative intensity of the less rhombic signal. These data demonstrate the sensitivitiy of the ligand field to changes in catalase solvent and, furthermore, suggest that both rhombic configuration posses identical spectral and catalytic properties. | Effect of pH on bovine liver catalase as determined by electron paramagnetic resonance. Two major rhombic high-spin ferric heme signals are observed during the pH titration of bovine liver catalase. The less rhombic signal if dominant above pH 6.0 and the more rhombic signal below pH 6.0. Ethanol in high concentration enhances the relative intensity of the less rhombic signal. These data demonstrate the sensitivitiy of the ligand field to changes in catalase solvent and, furthermore, suggest that both rhombic configuration posses identical spectral and catalytic properties. | [
-0.047610461711883545,
0.05182946100831032,
-0.0627494752407074,
0.040853582322597504,
0.03403385728597641,
-0.06637988984584808,
0.07690346240997314,
0.056040529161691666,
-0.00665638642385602,
-0.011843093670904636,
0.02259320765733719,
-0.04800393432378769,
-0.006112704519182444,
0.051220133900642395,
0.000469760678242892,
-0.037197504192590714,
-0.013057832606136799,
0.040904875844717026,
-0.07394733279943466,
0.002730022883042693,
0.011054211296141148,
0.03838434815406799,
0.014499290846288204,
0.04228551685810089,
0.05988229066133499,
0.03934406861662865,
0.022967154160141945,
0.025345822796225548,
-0.07902350276708603,
-0.20636004209518433,
0.06074133887887001,
-0.022636504843831062,
0.036420322954654694,
-0.008224532939493656,
0.022111469879746437,
0.018860304728150368,
-0.04501131549477577,
-0.03531968221068382,
-0.055339861661195755,
0.008696744218468666,
0.002467215061187744,
-0.0002940082922577858,
-0.007320919539779425,
0.06987113505601883,
-0.04439897462725639,
0.017692653462290764,
0.009602732956409454,
0.02130013331770897,
0.014967313967645168,
-0.003994381986558437,
-0.004293536767363548,
0.017808234319090843,
-0.023475296795368195,
-0.0005546355969272554,
0.036487776786088943,
0.0029820536728948355,
0.059069328010082245,
0.04856989532709122,
-0.013590492308139801,
0.005952872801572084,
0.01363124419003725,
0.024493610486388206,
-0.15650302171707153,
0.053992994129657745,
0.07065216451883316,
-0.022230448201298714,
-0.023964304476976395,
-0.039394330233335495,
-0.0007112697931006551,
0.07684040069580078,
-0.07644011825323105,
0.00823261309415102,
-0.04801987111568451,
-0.03420637175440788,
0.058570489287376404,
-0.07604299485683441,
-0.018185192719101906,
-0.05162982642650604,
-0.00578811950981617,
0.015682807192206383,
-0.02357008494436741,
0.013185073621571064,
0.0014758900506421924,
-0.037161532789468765,
0.012805402278900146,
0.011103980243206024,
-0.009643788449466228,
-0.012472393922507763,
-0.008393900468945503,
0.02136826515197754,
-0.011462576687335968,
-0.07964035123586655,
-0.01764865778386593,
-0.022293832153081894,
-0.09768307954072952,
0.096701480448246,
0.02339463122189045,
-0.0005679781315848231,
-0.00263594719581306,
0.42001616954803467,
0.0008328296826221049,
-0.007493917364627123,
-0.05938956141471863,
-0.012956171296536922,
-0.009804774075746536,
-0.030912157148122787,
-0.027705596759915352,
0.029485546052455902,
-0.0412447527050972,
0.04949220269918442,
0.014946608804166317,
-0.028574490919709206,
0.017374953255057335,
-0.05259697884321213,
-0.04192252829670906,
0.03371479734778404,
0.03359181433916092,
0.00921468436717987,
0.002467197133228183,
0.017027126625180244,
-0.019282525405287743,
-0.01914793811738491,
0.06949096918106079,
0.0013902782229706645,
0.058864012360572815,
-0.025937847793102264,
0.02433277666568756,
0.09262111037969589,
0.034716758877038956,
0.05895739048719406,
0.001987162744626403,
0.015816297382116318,
0.020384181290864944,
-0.01983419805765152,
-0.03035230003297329,
0.0027990457601845264,
0.05033193901181221,
-0.013996722176671028,
0.02940080314874649,
0.07631729543209076,
-0.0859176367521286,
-0.05083088204264641,
-0.06028196960687637,
-0.05524788796901703,
-0.027633069083094597,
0.002458123257383704,
0.022643137723207474,
0.0000020526915704977,
-0.05731874704360962,
-0.00711834616959095,
0.024306178092956543,
0.0587046779692173,
-0.058612290769815445,
0.0567517951130867,
-0.0021421911660581827,
-0.007517618127167225,
-0.0578194297850132,
-0.031171176582574844,
0.03290007263422012,
-0.03705977648496628,
-0.08698111772537231,
-0.043352045118808746,
-0.002531809266656637,
0.06292200833559036,
-0.010148844681680202,
-0.02111777476966381,
-0.01513352245092392,
-0.03658479079604149,
0.038572557270526886,
0.00799934659153223,
0.007194719277322292,
0.010965021327137947,
0.013849794864654541,
-0.08020135015249252,
-0.047389913350343704,
0.036495815962553024,
0.04850802943110466,
0.05376749113202095,
0.01305088959634304,
-0.02561618760228157,
0.09488800913095474,
0.044232845306396484,
-0.016902726143598557,
0.03897009789943695,
0.009264979511499405,
-0.023752102628350258,
-0.04842127487063408,
-0.012487201020121574,
-0.025297213345766068,
-0.004228721372783184,
-0.030191779136657715,
0.015160303562879562,
-0.05219583585858345,
0.01963851973414421,
0.0036215458530932665,
-0.06477165967226028,
0.004899015184491873,
0.007718220818787813,
0.019799301400780678,
-0.015131698921322823,
0.0871448963880539,
-0.008472220040857792,
-0.0396454781293869,
0.03103731758892536,
0.027364997193217278,
-0.01335892640054226,
0.04123736545443535,
0.07008448988199234,
0.0059142387472093105,
0.0065689715556800365,
0.005894607864320278,
0.02001768909394741,
-0.06338749080896378,
-0.02964952029287815,
-0.07505406439304352,
-0.016823863610625267,
0.03280647099018097,
0.03578830882906914,
0.05811313912272453,
0.024573087692260742,
0.031338922679424286,
-0.10045313090085983,
-0.004974752198904753,
-0.2779824435710907,
-0.015001321211457253,
0.016506638377904892,
-0.03192335367202759,
0.035535864531993866,
-0.04858201742172241,
0.005162219516932964,
-0.0031595537438988686,
0.0618661530315876,
0.0068840873427689075,
0.06292039901018143,
0.08429227024316788,
-0.058620352298021317,
-0.05096752941608429,
0.03358783945441246,
0.0423782616853714,
0.039067186415195465,
-0.051239438354969025,
0.029872337356209755,
0.060673341155052185,
0.030494138598442078,
-0.001082805683836341,
0.01428463589400053,
0.01179424300789833,
0.0369948148727417,
0.01136078406125307,
0.09941480308771133,
-0.0011750084813684225,
-0.010635901242494583,
0.0035978732630610466,
-0.025162819772958755,
0.03600893169641495,
0.001213615294545889,
-0.05928614363074303,
-0.016501102596521378,
0.019868554547429085,
0.02527736872434616,
-0.0046972185373306274,
-0.0019778700079768896,
-0.014114657416939735,
-0.025278981775045395,
0.0067643518559634686,
-0.048253946006298065,
-0.036747440695762634,
-0.05621195584535599,
0.012932956218719482,
0.015938064083456993,
0.004550481680780649,
0.0015308891888707876,
0.002390429610386491,
0.07624128460884094,
-0.03696756437420845,
0.02888779528439045,
-0.013239496387541294,
-0.011643383651971817,
-0.06111347675323486,
0.006047096103429794,
0.049100883305072784,
-0.03910871595144272,
-0.055396903306245804,
-0.02076217532157898,
-0.045922063291072845,
-0.02262965962290764,
0.058579277247190475,
0.007897025905549526,
-0.004192524589598179,
0.013387463986873627,
-0.0011270809918642044,
0.0620376318693161,
0.003029149491339922,
0.020106380805373192,
0.08091108500957489,
-0.06494738161563873,
0.033553458750247955,
0.029323361814022064,
-0.013330741785466671,
-0.0013220298569649458,
-0.004910876043140888,
-0.029521964490413666,
0.022678697481751442,
-0.031599871814250946,
0.025501523166894913,
0.05492297559976578,
-0.004122828599065542,
-0.008608429692685604,
0.039719969034194946,
-0.0008997506811283529,
-0.06913930177688599,
0.008830749429762363,
0.02710307016968727,
0.02754802815616131,
-0.018709663301706314,
-0.0661218911409378,
-0.052946537733078,
0.033738963305950165,
0.03360689431428909,
-0.23424068093299866,
-0.004084225744009018,
0.049224212765693665,
0.023401709273457527,
-0.05529407784342766,
0.06373710185289383,
0.04351603239774704,
-0.04464404284954071,
-0.051517922431230545,
0.023484600707888603,
-0.017400605604052544,
0.04075833782553673,
0.07150278240442276,
0.003339794697239995,
0.018874801695346832,
-0.0027620424516499043,
0.009292342700064182,
-0.06857243925333023,
-0.0294467993080616,
-0.0004701701982412487,
0.009721783921122551,
-0.019647888839244843,
0.173765629529953,
-0.03879809379577637,
-0.06097619980573654,
0.014430763199925423,
0.014869864098727703,
0.006678882520645857,
-0.04132635146379471,
-0.02772773802280426,
0.00331378192640841,
0.01635243557393551,
0.045414116233587265,
-0.029238803312182426,
0.04260456934571266,
0.08424969762563705,
-0.007605347782373428,
-0.038458362221717834,
-0.009449267759919167,
0.03407015651464462,
0.027733514085412025,
0.032827671617269516,
-0.11197376251220703,
-0.07226745039224625,
0.00443223724141717,
-0.04214395582675934,
-0.07304410636425018,
0.0029457404743880033,
0.05170968174934387,
-0.0288095586001873,
-0.03898806497454643,
0.01961144432425499,
-0.01668582111597061,
0.06973262876272202,
-0.04693099111318588,
0.0447685606777668,
-0.05464882031083107,
-0.03540289029479027,
-0.012536303140223026,
-0.00898722279816866,
0.01893097162246704,
0.07660166919231415,
-0.00559734832495451,
0.05018731579184532,
0.02484872192144394
] |
PMID:27229 | Studies on erythrocruorin. VII. Reconstitution of earthworm erythrocruorin from the apoprotein. | Apoerythrocruorin prepared from the giant respiratory hemoprotein of the earthworm (60 S, Mr = 3 X 10(-6)) is an electrophoretically homogeneous molecule which sediments as a single peak of low molecular weight (3.5 S) and has a lower alpha-helical content (approx. 30%) than the native protein. Titration of globin with ferric heme indicates the presence of different binding sites; however, after purification by ion exchange chromatography, the reconstitution product contains 1 haem/23 000 g of protein as the native molecule. Reconstituted ferric erythrocruorin is a low molecular weight hemichrome with the same optical and physicochemical properties of the hemichrome formed by natural ferric erythrocruorin. Reconstituted ferrous erythrocruorin reacquires the alpha-helical content and the quaternary structure of the native molecule. Reassociation into 10-S speices (1/12 of the whole molecules) is fast and easy, while that into whole molecules is slow and somewhat erratic. The functional properties of reconstituted ferrous erythrocruorin (oxygen affinity, cooperativity in oxygen binding, magnitude of Bohr effect) are very similar to those of the "stable" low cooperativity form of the undissociated protein. | Studies on erythrocruorin. VII. Reconstitution of earthworm erythrocruorin from the apoprotein. Apoerythrocruorin prepared from the giant respiratory hemoprotein of the earthworm (60 S, Mr = 3 X 10(-6)) is an electrophoretically homogeneous molecule which sediments as a single peak of low molecular weight (3.5 S) and has a lower alpha-helical content (approx. 30%) than the native protein. Titration of globin with ferric heme indicates the presence of different binding sites; however, after purification by ion exchange chromatography, the reconstitution product contains 1 haem/23 000 g of protein as the native molecule. Reconstituted ferric erythrocruorin is a low molecular weight hemichrome with the same optical and physicochemical properties of the hemichrome formed by natural ferric erythrocruorin. Reconstituted ferrous erythrocruorin reacquires the alpha-helical content and the quaternary structure of the native molecule. Reassociation into 10-S speices (1/12 of the whole molecules) is fast and easy, while that into whole molecules is slow and somewhat erratic. The functional properties of reconstituted ferrous erythrocruorin (oxygen affinity, cooperativity in oxygen binding, magnitude of Bohr effect) are very similar to those of the "stable" low cooperativity form of the undissociated protein. | [
-0.09222135692834854,
-0.03838532045483589,
-0.04197436943650246,
-0.02042403444647789,
0.0035213062074035406,
-0.05822934955358505,
0.05742773413658142,
0.024611741304397583,
-0.09946252405643463,
0.015962136909365654,
0.03834351897239685,
-0.0751463994383812,
-0.0028016106225550175,
0.03164471313357353,
-0.06314971297979355,
-0.01707981899380684,
-0.0302297193557024,
0.05602511391043663,
-0.07215433567762375,
0.062167923897504807,
-0.0019696310628205538,
-0.06232205405831337,
0.031067049130797386,
-0.001743989298120141,
0.03679708018898964,
0.11038072407245636,
-0.07182218134403229,
-0.011046482250094414,
-0.008705971762537956,
-0.20282761752605438,
0.017670661211013794,
-0.01077597588300705,
0.011641834862530231,
0.030352961272001266,
0.008824838325381279,
0.0025621491950005293,
-0.049255289137363434,
-0.05854490026831627,
-0.07852756232023239,
0.04421013593673706,
0.018737630918622017,
0.0773463100194931,
-0.02160174399614334,
0.006030766759067774,
0.03483623266220093,
0.008366282097995281,
-0.004335592966526747,
-0.008084844797849655,
0.039791256189346313,
0.07336725294589996,
0.04067523032426834,
0.031514108180999756,
-0.011030268855392933,
0.030860422179102898,
0.03639421984553337,
0.054003071039915085,
0.014378751628100872,
0.07570592314004898,
-0.015486031770706177,
0.024531060829758644,
0.0010237552924081683,
0.06776811927556992,
-0.10881384462118149,
0.05762837454676628,
0.09453684836626053,
0.015978654846549034,
-0.012977270409464836,
-0.09168180078268051,
0.04346098378300667,
0.04188629984855652,
-0.039965346455574036,
0.06533876061439514,
0.009870423935353756,
0.018728893250226974,
-0.028128761798143387,
0.011015035212039948,
-0.006961663719266653,
-0.010153055191040039,
0.03877236321568489,
0.0037741216365247965,
-0.008941926062107086,
0.0706646516919136,
0.007974616251885891,
-0.010690031573176384,
0.003644300391897559,
0.026179855689406395,
-0.019694795832037926,
0.0015420446870848536,
0.012676904909312725,
0.04047118127346039,
-0.004614174831658602,
-0.03522523492574692,
-0.0030553576070815325,
-0.03374570980668068,
-0.09543686360120773,
-0.01070709340274334,
-0.004194187931716442,
0.021889273077249527,
0.03972037136554718,
0.3513535261154175,
-0.062171075493097305,
0.05802358314394951,
-0.05755515396595001,
0.0021053196396678686,
0.038887426257133484,
-0.014158988371491432,
0.01727505587041378,
0.018243880942463875,
0.004737074952572584,
0.049496300518512726,
-0.0020625111646950245,
-0.003486820263788104,
0.036081165075302124,
-0.028473224490880966,
-0.009189200587570667,
-0.008679402060806751,
-0.017746595665812492,
-0.0009464371250942349,
-0.06523033231496811,
0.057898420840501785,
-0.03055000677704811,
-0.01123056747019291,
0.010509239509701729,
0.00728190504014492,
0.07081326842308044,
0.009975039400160313,
-0.0014202742604538798,
0.04486515000462532,
0.04595493897795677,
0.020767033100128174,
0.02214088663458824,
0.05357047915458679,
0.0012766380095854402,
-0.028505176305770874,
-0.003421199042350054,
0.03256434574723244,
0.032863933593034744,
-0.053868021816015244,
-0.003836090676486492,
0.046699777245521545,
-0.030889548361301422,
-0.004726502578705549,
-0.06645829975605011,
-0.04000621661543846,
-0.07807446271181107,
0.055091727524995804,
0.0005921878619119525,
0.02729138359427452,
-0.035184383392333984,
-0.006591262295842171,
0.01905839703977108,
0.04908866062760353,
0.005417747888714075,
0.020527129992842674,
0.0025221966207027435,
0.03331362456083298,
-0.00959501788020134,
-0.016283376142382622,
0.0012748322915285826,
-0.02056955359876156,
-0.07463368028402328,
0.04999448359012604,
-0.014718745835125446,
0.10223610699176788,
0.0011311514535918832,
-0.05525154992938042,
-0.014056460931897163,
0.0038432441651821136,
0.016535257920622826,
-0.02734624221920967,
-0.01254511158913374,
-0.010586638003587723,
-0.0006848174380138516,
-0.06268090754747391,
0.008752859197556973,
0.052354928106069565,
0.028245599940419197,
-0.05675463005900383,
0.07604166120290756,
-0.015465191565454006,
0.010815136134624481,
-0.008887691423296928,
-0.053088873624801636,
0.00840391218662262,
0.0211225263774395,
-0.03314874321222305,
-0.09002479165792465,
0.022798188030719757,
-0.050535883754491806,
0.06850890815258026,
-0.058076176792383194,
0.03359367325901985,
0.018794137984514236,
0.05712099000811577,
-0.020929496735334396,
-0.05686897784471512,
-0.022853195667266846,
-0.02900162898004055,
-0.00012391201744321734,
0.0003560871700756252,
0.1413264125585556,
-0.00023134841467253864,
0.017237605527043343,
0.03776976093649864,
0.022014383226633072,
0.0026391588617116213,
0.044986363500356674,
0.027268262580037117,
0.021893266588449478,
-0.00550907664000988,
0.025020688772201538,
-0.005085153970867395,
-0.07253801822662354,
-0.0005958564579486847,
-0.05425558239221573,
0.004492098465561867,
0.038131196051836014,
-0.003714134218171239,
0.08030272275209427,
0.003921411000192165,
-0.013921057805418968,
-0.10295446962118149,
-0.01869046315550804,
-0.3008788228034973,
-0.010216129012405872,
-0.024981187656521797,
-0.08672481775283813,
0.06074937433004379,
-0.03234727680683136,
0.011476411484181881,
0.017221670597791672,
0.06407976895570755,
-0.0403754860162735,
0.06199634447693825,
0.06433640420436859,
-0.007364911027252674,
-0.06691460311412811,
-0.002620645333081484,
0.039498280733823776,
0.07233388721942902,
-0.045243941247463226,
0.025583093985915184,
0.05300002917647362,
0.008455631323158741,
-0.008869213052093983,
-0.02227795124053955,
0.02604844979941845,
0.026501687243580818,
-0.04378523677587509,
0.1417221873998642,
0.004632934927940369,
-0.02471124567091465,
-0.03196124732494354,
-0.02163604088127613,
0.01562534086406231,
0.01564725674688816,
-0.040183331817388535,
0.013499340042471886,
-0.007533201947808266,
-0.0033369907177984715,
-0.024476638063788414,
0.014952462166547775,
-0.03294559568166733,
-0.005697098560631275,
-0.016242390498518944,
-0.0012255680048838258,
-0.07351789623498917,
-0.024536821991205215,
-0.0246605034917593,
-0.06425444781780243,
-0.008149683475494385,
0.05195595324039459,
0.023319954052567482,
0.06951455771923065,
-0.01523943804204464,
0.02966742217540741,
0.03570299595594406,
-0.031870365142822266,
0.013483880087733269,
-0.023588014766573906,
0.013959542848169804,
-0.05599771812558174,
-0.02107318677008152,
0.06137235462665558,
0.011647467501461506,
-0.010211446322500706,
0.025437666103243828,
0.03179548308253288,
0.007094037719070911,
0.048706889152526855,
-0.06440255790948868,
0.09360522776842117,
0.021255990490317345,
-0.009027618914842606,
0.10213053971529007,
-0.007584467995911837,
0.01431558933109045,
0.005743773654103279,
0.01516055129468441,
-0.01794462651014328,
-0.0031029530800879,
-0.02521771937608719,
0.03901982307434082,
0.02626502327620983,
0.009944354183971882,
0.02329830639064312,
0.007618731819093227,
-0.0014109259936958551,
-0.016744785010814667,
-0.024872614070773125,
0.013421368785202503,
-0.041884396225214005,
0.04339485242962837,
-0.0028116777539253235,
0.0030064894817769527,
-0.053785983473062515,
-0.025577407330274582,
-0.02307434193789959,
0.07480436563491821,
-0.30548202991485596,
0.02745780535042286,
0.015686379745602608,
0.05108308047056198,
-0.01767050102353096,
-0.004523835610598326,
0.04941058158874512,
-0.04765890911221504,
-0.0134371193125844,
-0.009359742514789104,
-0.010432166047394276,
-0.0030543531756848097,
0.03657044097781181,
0.01978149078786373,
-0.027283187955617905,
0.036305006593465805,
0.023749297484755516,
-0.01733926124870777,
-0.02120230160653591,
-0.01635638251900673,
-0.022493524476885796,
0.011759081855416298,
0.1396520435810089,
-0.010946938768029213,
-0.05541021376848221,
-0.000665738363750279,
-0.01502532884478569,
0.025391878560185432,
-0.02927420847117901,
0.009393327869474888,
0.03723563253879547,
0.0015085266204550862,
0.0634109303355217,
-0.07618369907140732,
-0.011348757892847061,
0.024283060804009438,
-0.02481590025126934,
-0.0433548167347908,
0.01742413640022278,
0.018239308148622513,
-0.04169217124581337,
0.02899417094886303,
-0.020060764625668526,
-0.041508421301841736,
0.08155331760644913,
-0.04417800158262253,
-0.06186912953853607,
-0.0332062765955925,
-0.021504059433937073,
-0.02800806611776352,
-0.06618553400039673,
-0.02769082970917225,
0.03875203803181648,
0.008974180556833744,
-0.03084511309862137,
0.0011367062106728554,
-0.03974516689777374,
0.02439386025071144,
-0.06960273534059525,
-0.01903308555483818,
0.013125989586114883,
-0.05808226764202118,
-0.08296465873718262,
0.07002489268779755,
0.014433219097554684
] |
PMID:27230 | Oxygen equilibrium analyses of isolated hemoglobins A2, Lepore-Washington and P-nilotic. | Oxygen equilibrium studies have been carried out on hemoglobins A2 (alpha2delta2), Lepore-Washington (alpha2(deltabeta)2) and P-Nilotic (alpha2(beta2delta)2) using the beta chain containing hemoglobins A and S as controls. This investigation was initiated mainly because of controversial data that have been published on the oxygen affinity of hemoglobin (Hb) A2 and because samples containing the rare Hb P-Nilotic became available. Each hemoglobin was isolated in pure form by anion exchange chromatography; the samples used in the equilibrium analyses contained 100 mg Hb/dl with less than 5% ferrihemoglobin and no 2,3--diphosphoglycerate. Oxygen equilibrium analyses were made at 37 degrees C with the method of Benesch et al. (1965) Anal. Biochem. 11, 81--87; Anal. Biochem. 55, 245--248 (1973). A slight, but definite increase in oxygen affinity was observed for Hb A2 as well as for Hb P-Nilotic while the increase for the Hb Lepore-Washington was somewhat greater. The values for n, the Hill coefficient, and the Bohr effects were the same for all hemoglobin types. The differences in oxygen affinity of these hemoglobins apparently result from the differences in primary structure that are characteristic for those proteins. | Oxygen equilibrium analyses of isolated hemoglobins A2, Lepore-Washington and P-nilotic. Oxygen equilibrium studies have been carried out on hemoglobins A2 (alpha2delta2), Lepore-Washington (alpha2(deltabeta)2) and P-Nilotic (alpha2(beta2delta)2) using the beta chain containing hemoglobins A and S as controls. This investigation was initiated mainly because of controversial data that have been published on the oxygen affinity of hemoglobin (Hb) A2 and because samples containing the rare Hb P-Nilotic became available. Each hemoglobin was isolated in pure form by anion exchange chromatography; the samples used in the equilibrium analyses contained 100 mg Hb/dl with less than 5% ferrihemoglobin and no 2,3--diphosphoglycerate. Oxygen equilibrium analyses were made at 37 degrees C with the method of Benesch et al. (1965) Anal. Biochem. 11, 81--87; Anal. Biochem. 55, 245--248 (1973). A slight, but definite increase in oxygen affinity was observed for Hb A2 as well as for Hb P-Nilotic while the increase for the Hb Lepore-Washington was somewhat greater. The values for n, the Hill coefficient, and the Bohr effects were the same for all hemoglobin types. The differences in oxygen affinity of these hemoglobins apparently result from the differences in primary structure that are characteristic for those proteins. | [
-0.09170167148113251,
0.052579931914806366,
-0.04598013684153557,
-0.011448044329881668,
0.026155686005949974,
-0.03794713690876961,
0.06382980197668076,
0.014039103873074055,
-0.04049736633896828,
0.02919480763375759,
0.04577445238828659,
-0.09024868905544281,
-0.03114371933043003,
0.020883653312921524,
-0.048091545701026917,
-0.04243497923016548,
0.0009348223102279007,
-0.041708700358867645,
-0.05189678445458412,
0.05365460366010666,
-0.05021267011761665,
-0.03991101309657097,
0.03685132414102554,
0.021851560100913048,
0.053220685571432114,
0.07600293308496475,
-0.029467053711414337,
-0.004669447895139456,
-0.01940632425248623,
-0.206861212849617,
-0.019336741417646408,
-0.010018475353717804,
-0.003647739300504327,
-0.041304972022771835,
-0.014419804327189922,
0.044609054923057556,
-0.039791520684957504,
0.018611706793308258,
-0.03643934801220894,
0.0247407965362072,
0.04813311994075775,
-0.007970505394041538,
0.008918714709579945,
0.06423400342464447,
0.024339977651834488,
-0.017778879031538963,
-0.03561033308506012,
0.013632562942802906,
0.05250265449285507,
0.013866419903934002,
0.007624354213476181,
0.011670558713376522,
-0.0026717903092503548,
-0.010803435929119587,
-0.02294357307255268,
-0.019994698464870453,
0.0170828178524971,
0.03352032229304314,
0.029164237901568413,
0.04416796937584877,
0.024079618975520134,
0.015336267650127411,
-0.14639857411384583,
0.05593765899538994,
0.09393735229969025,
0.024959087371826172,
-0.00897752121090889,
-0.051630254834890366,
0.0553743876516819,
-0.00039047549944370985,
0.020488528534770012,
0.060078516602516174,
0.023675765842199326,
-0.014212138950824738,
-0.016218021512031555,
0.00925877783447504,
0.019148534163832664,
-0.0446225181221962,
0.04031513258814812,
0.007489924319088459,
-0.01246671937406063,
0.022376324981451035,
0.026867756620049477,
-0.009236383251845837,
0.02153935469686985,
-0.015159553848206997,
0.007254108786582947,
0.0040949988178908825,
-0.06250006705522537,
-0.030147474259138107,
-0.011995705775916576,
-0.057084932923316956,
-0.041253332048654556,
0.03507605567574501,
-0.05396908521652222,
-0.013140838593244553,
-0.005265471525490284,
-0.000710070482455194,
0.014915163628757,
0.4199184775352478,
-0.019358495250344276,
0.023842884227633476,
0.0034108133986592293,
0.015518463216722012,
0.019021116197109222,
-0.053686294704675674,
0.0002631576207932085,
-0.022863244637846947,
-0.013757583685219288,
0.017709901556372643,
0.0801922008395195,
-0.012328017503023148,
0.05380465090274811,
-0.03458881378173828,
-0.028408830985426903,
0.009645734913647175,
0.02016099914908409,
-0.005276795942336321,
-0.05439867079257965,
0.060155775398015976,
-0.07999767363071442,
-0.003988435957580805,
0.04195713624358177,
0.001571119762957096,
0.03527260199189186,
-0.0057754856534302235,
0.002843121998012066,
0.07908215373754501,
0.1152525320649147,
-0.0022806022316217422,
0.06808289885520935,
0.0273891594260931,
-0.05456138402223587,
-0.0336601696908474,
-0.09972625970840454,
-0.006916510406881571,
0.03359164297580719,
-0.01578621007502079,
0.035776954144239426,
0.005498610902577639,
-0.024249151349067688,
-0.048431918025016785,
-0.01328082475811243,
-0.06058376282453537,
-0.09217002242803574,
0.0860433354973793,
0.025351401418447495,
0.0059486255049705505,
-0.03513467684388161,
-0.015202064998447895,
-0.04081689193844795,
0.03682955354452133,
-0.006821244489401579,
0.014668489806354046,
-0.0037564190570265055,
0.04473837465047836,
-0.01005355454981327,
0.004912762437015772,
0.03204973787069321,
0.003834022209048271,
-0.07523626834154129,
0.007030540611594915,
0.017833227291703224,
0.014427486807107925,
-0.003184674307703972,
-0.024891147390007973,
-0.015256810002028942,
0.0009126302902586758,
0.018791312351822853,
-0.02922786772251129,
0.05316583067178726,
-0.020447179675102234,
0.010902256704866886,
-0.024329211562871933,
-0.018588431179523468,
0.02615853026509285,
-0.0026332559064030647,
0.014002509415149689,
0.05711614340543747,
0.028198905289173126,
0.03870547562837601,
0.032530855387449265,
-0.03185562044382095,
-0.004724438767880201,
0.03617032244801521,
-0.022674119099974632,
-0.11242668330669403,
0.036183912307024,
-0.002599539002403617,
0.1034330427646637,
-0.07121731340885162,
-0.008035000413656235,
-0.04417155683040619,
0.057011593133211136,
-0.006060560699552298,
-0.007698697037994862,
-0.029246972873806953,
0.04012727364897728,
-0.021305182948708534,
-0.05117425695061684,
0.11343059688806534,
-0.006224983371794224,
-0.031128961592912674,
0.03930635377764702,
0.06884235888719559,
-0.010168553330004215,
0.022373266518115997,
-0.015031138435006142,
0.030315963551402092,
-0.002819822868332267,
-0.000683255901094526,
0.03202693164348602,
-0.09053938835859299,
-0.008582400158047676,
-0.027670320123434067,
-0.02183770202100277,
0.003799956990405917,
-0.024990063160657883,
0.0493040569126606,
0.012570380233228207,
0.036193087697029114,
-0.09984078258275986,
-0.018363090232014656,
-0.31262296438217163,
-0.005486223381012678,
0.020275048911571503,
-0.019162015989422798,
0.03691575303673744,
0.017011474817991257,
0.038530223071575165,
-0.002009298885241151,
-0.04281165450811386,
0.011787804774940014,
0.01227717474102974,
0.061373598873615265,
-0.08745905011892319,
-0.03493097424507141,
0.0018126382492482662,
0.05581037327647209,
0.08375979959964752,
-0.03963103145360947,
-0.005832626949995756,
0.05192277580499649,
0.05790108069777489,
-0.033669620752334595,
-0.03330885246396065,
0.034654147922992706,
0.028628526255488396,
0.016866443678736687,
0.07641538232564926,
-0.0860222801566124,
0.009568874724209309,
0.006718697026371956,
0.03100416623055935,
0.03199296444654465,
0.014857103116810322,
-0.01648871786892414,
-0.022582415491342545,
-0.06046377494931221,
-0.03139477223157883,
-0.009686393663287163,
0.04322481155395508,
0.006063203327357769,
0.0013649227330461144,
0.029651198536157608,
0.023511547595262527,
-0.028646888211369514,
-0.008083103224635124,
0.014423263259232044,
0.017043450847268105,
-0.020098231732845306,
-0.04748724028468132,
-0.03996353968977928,
0.03159923851490021,
0.0043800873681902885,
-0.003322361269965768,
0.013291922397911549,
0.0293799489736557,
0.011378057301044464,
-0.060732919722795486,
0.018707938492298126,
-0.04847420006990433,
-0.012188485823571682,
-0.025208599865436554,
-0.04055699706077576,
-0.020890433341264725,
0.009509121999144554,
-0.00925753079354763,
0.0071084932424128056,
0.06699758768081665,
-0.023164570331573486,
0.05076456069946289,
0.0007895023445598781,
0.026150811463594437,
0.02139248698949814,
-0.048758622258901596,
0.0009953597327694297,
0.01006235834211111,
0.0672740638256073,
-0.013310873880982399,
-0.017877545207738876,
-0.04569806531071663,
-0.023634396493434906,
0.0075509981252253056,
0.010207143612205982,
0.05072178691625595,
0.0407693050801754,
-0.03289831802248955,
0.0730445384979248,
0.05718735605478287,
-0.03470248728990555,
-0.0038540558889508247,
0.011232702061533928,
-0.02166052535176277,
-0.04086408019065857,
-0.014485534280538559,
-0.03291601315140724,
0.04332007095217705,
0.05067751184105873,
-0.268504798412323,
-0.035204630345106125,
0.01993587613105774,
0.035107582807540894,
-0.020231790840625763,
0.008575869724154472,
0.05038220062851906,
-0.05224195122718811,
-0.01892145723104477,
0.01756945252418518,
0.010456128977239132,
0.01327994093298912,
0.03268120065331459,
0.00155824760440737,
0.0000761529736337252,
0.0200213473290205,
0.07929321378469467,
-0.032215435057878494,
0.04993557929992676,
-0.012177144177258015,
-0.03805903345346451,
-0.027076905593276024,
0.14075236022472382,
-0.04199088737368584,
-0.049107421189546585,
0.0006571469712071121,
-0.03810841590166092,
0.05344738811254501,
-0.040233440697193146,
0.03622068464756012,
0.04462016001343727,
0.005218569654971361,
0.07316558063030243,
-0.06479103118181229,
-0.00614137714728713,
0.052858974784612656,
-0.06366807222366333,
-0.016756204888224602,
-0.01173988077789545,
-0.019904661923646927,
0.019152754917740822,
0.02323783189058304,
-0.09507136791944504,
0.0028218068182468414,
0.0794762372970581,
-0.018770352005958557,
-0.0808335542678833,
-0.004867202136665583,
0.005679921247065067,
-0.006345182657241821,
-0.03221432864665985,
-0.026555746793746948,
0.03631485253572464,
0.04616273567080498,
0.017075026407837868,
-0.03887323662638664,
-0.039865925908088684,
-0.013511676341295242,
-0.004268409684300423,
-0.011515608057379723,
0.03256910666823387,
0.03134124353528023,
-0.07417776435613632,
0.028366364538669586,
0.03272944688796997
] |
PMID:27231 | The cleavage and adsorption of parathyroid hormone at high dilution: implications for receptor binding studies. | Like other polypeptide hormones, purified intact parathyroid hormone (1-84)parathyroid hormone is notoriously unstable and is subject to large adsorptive losses in routine laboratory manipulation. The present studies were undertaken with 125I-labeled hormone to quantitate the problem and to develop preventative measures, particularly with concentrations of physiological interest, 1 . 10(-10) M. It was found that spontaneous cleavage of the hormone takes place upon its incubation in air or oxygen. This can be prevented by the presence of mercaptoethanol or by plasma levels of cysteine and ascorbate. Under non-cleavage conditions, adsorption was found to be extensive on all materials tested. This adsorption increased with time up to 2 h, was independent of ionic strength, increased with increasing temperature and was presumed to involve hydrophobic interactions. Under given conditions, adsorption was proportional to concentration (constant percentage). However, at very high concentrations, 1 . 10(-6) M, adsorption was markedly reduced. Adsorption was minimized at low pH (2). Bovine serum albumin reduced adsorption under all conditions when present at concentrations of 2 mg/ml or more. Coating laboratory ware with cetyl alcohol also was helpful. Using optimal conditions, cleavage is prevented and losses are less than 5% at neutral pH, and under 2% at pH 2. | The cleavage and adsorption of parathyroid hormone at high dilution: implications for receptor binding studies. Like other polypeptide hormones, purified intact parathyroid hormone (1-84)parathyroid hormone is notoriously unstable and is subject to large adsorptive losses in routine laboratory manipulation. The present studies were undertaken with 125I-labeled hormone to quantitate the problem and to develop preventative measures, particularly with concentrations of physiological interest, 1 . 10(-10) M. It was found that spontaneous cleavage of the hormone takes place upon its incubation in air or oxygen. This can be prevented by the presence of mercaptoethanol or by plasma levels of cysteine and ascorbate. Under non-cleavage conditions, adsorption was found to be extensive on all materials tested. This adsorption increased with time up to 2 h, was independent of ionic strength, increased with increasing temperature and was presumed to involve hydrophobic interactions. Under given conditions, adsorption was proportional to concentration (constant percentage). However, at very high concentrations, 1 . 10(-6) M, adsorption was markedly reduced. Adsorption was minimized at low pH (2). Bovine serum albumin reduced adsorption under all conditions when present at concentrations of 2 mg/ml or more. Coating laboratory ware with cetyl alcohol also was helpful. Using optimal conditions, cleavage is prevented and losses are less than 5% at neutral pH, and under 2% at pH 2. | [
-0.02745986357331276,
0.012111085467040539,
-0.02526973932981491,
0.05723122879862785,
0.013971124775707722,
-0.03825487941503525,
0.06690669804811478,
0.06039538234472275,
-0.06142181158065796,
-0.0246810931712389,
0.019919078797101974,
-0.06631916761398315,
0.07359045743942261,
0.06754712760448456,
0.02125140093266964,
0.0010100624058395624,
0.05571671202778816,
0.005060942843556404,
-0.026608802378177643,
0.005878983065485954,
0.028573976829648018,
-0.01819535344839096,
0.02485145628452301,
0.020406309515237808,
0.014471076428890228,
0.04537020996212959,
-0.02926717698574066,
-0.004544323310256004,
-0.06217297911643982,
-0.1909642368555069,
0.020752059295773506,
-0.03241204097867012,
0.05462398752570152,
-0.037468813359737396,
0.008043061941862106,
0.002742731012403965,
-0.014018634334206581,
-0.007672580424696207,
0.0034933181013911963,
0.042126789689064026,
0.08729007840156555,
-0.0024777548387646675,
-0.09089578688144684,
0.0313766784965992,
0.022080348804593086,
0.06266295909881592,
-0.07219265401363373,
0.0064146192744374275,
0.07931847870349884,
0.04898834601044655,
-0.04924093931913376,
0.04267159104347229,
-0.015463905408978462,
0.04631010815501213,
0.0006914832629263401,
-0.04532798379659653,
0.017908679321408272,
0.04992213472723961,
0.008190355263650417,
0.058153100311756134,
0.01382916234433651,
0.028904495760798454,
-0.09394563734531403,
0.06009066477417946,
0.07865208387374878,
0.04967659339308739,
-0.0004756488197017461,
-0.01125503983348608,
0.026121599599719048,
0.04920293763279915,
-0.0010700839338824153,
0.034517861902713776,
0.008901514112949371,
0.023166226223111153,
-0.009652331471443176,
-0.0372273325920105,
-0.002593555487692356,
-0.043520327657461166,
0.03620584309101105,
0.01206929236650467,
-0.013822736218571663,
0.03471599891781807,
0.050137344747781754,
-0.0004214900836814195,
-0.0479336753487587,
-0.012117920443415642,
-0.028611676767468452,
-0.006676682736724615,
0.022629987448453903,
0.021672822535037994,
-0.023764513432979584,
-0.01823228970170021,
0.003402658272534609,
0.021233905106782913,
-0.027534695342183113,
0.04654661938548088,
0.013102807104587555,
0.004798507783561945,
-0.0049026599153876305,
0.4036754071712494,
-0.01797337830066681,
0.03458995372056961,
-0.1224825456738472,
-0.037526458501815796,
0.013361304998397827,
-0.008851089514791965,
-0.013056064955890179,
0.008230140432715416,
-0.01769270747900009,
0.005784481763839722,
0.010672247968614101,
-0.00892656296491623,
0.012412458658218384,
-0.06829363852739334,
0.02380784973502159,
0.0015842467546463013,
0.010798982344567776,
-0.025385158136487007,
-0.040268104523420334,
-0.0342751145362854,
-0.02161097712814808,
0.013738594017922878,
0.010037482716143131,
-0.015282254666090012,
0.017234019935131073,
-0.01993812993168831,
0.02093198150396347,
0.06866536289453506,
0.03737756982445717,
0.013616999611258507,
0.021981149911880493,
-0.029516682028770447,
-0.066570945084095,
0.005202804692089558,
0.05201254412531853,
-0.017622046172618866,
0.015002270229160786,
-0.05958886817097664,
0.028533518314361572,
0.009516707621514797,
-0.0480816513299942,
-0.04440987482666969,
-0.00607434194535017,
-0.08543570339679718,
0.02540232613682747,
0.07415041327476501,
0.013084427453577518,
0.01964414119720459,
-0.007924889214336872,
-0.011950445361435413,
-0.030447080731391907,
0.05547570809721947,
-0.07101524621248245,
0.050598327070474625,
-0.012070880271494389,
-0.017222750931978226,
-0.03215211629867554,
-0.04466649517416954,
0.004951165523380041,
-0.02182987704873085,
-0.04124952107667923,
0.012459754943847656,
-0.02679440751671791,
-0.005522613413631916,
-0.0004508272686507553,
0.0072271921671926975,
-0.030995847657322884,
0.0009608250693418086,
0.05055144429206848,
0.0334145687520504,
-0.004613720811903477,
0.003151258686557412,
-0.005496539641171694,
-0.027870237827301025,
0.00387829658575356,
0.011011766269803047,
-0.027170879766345024,
-0.007201234810054302,
0.007626229431480169,
-0.029026130214333534,
-0.02168385684490204,
-0.0160402562469244,
-0.02269045077264309,
0.015078968368470669,
0.0487811379134655,
-0.06557539105415344,
-0.04075772315263748,
0.015417908318340778,
-0.018723176792263985,
0.04657455533742905,
-0.014465113170444965,
-0.009026152081787586,
-0.10472050309181213,
0.0011216066777706146,
-0.048916369676589966,
-0.03723864257335663,
-0.0313413068652153,
0.026731831952929497,
-0.013358033262193203,
-0.01556738093495369,
0.09615977853536606,
-0.0023439822252839804,
-0.015952950343489647,
0.05992845818400383,
0.052580326795578,
0.05943206697702408,
-0.0019064414082095027,
0.012921822257339954,
0.04932672902941704,
-0.011232866905629635,
-0.013975870795547962,
0.001796057098545134,
-0.08293543010950089,
-0.006953861098736525,
-0.014227278530597687,
-0.007389944978058338,
0.05005935952067375,
0.0006865122704766691,
0.04281703755259514,
-0.007826159708201885,
0.0526113361120224,
-0.03650446608662605,
-0.017782874405384064,
-0.3506697416305542,
-0.00662850821390748,
0.010711953043937683,
-0.0075265755876898766,
0.09172449260950089,
-0.06158360466361046,
-0.030596332624554634,
-0.03323162719607353,
-0.025436220690608025,
-0.03685633838176727,
0.053894657641649246,
0.059478212147951126,
-0.03836166486144066,
-0.059342388063669205,
0.014038272202014923,
0.04763470217585564,
0.060974206775426865,
0.024046702310442924,
0.0237367395311594,
-0.02603473886847496,
0.004563148133456707,
0.03146229311823845,
-0.016658902168273926,
0.0807855948805809,
0.019341332837939262,
-0.014967099763453007,
0.10917086154222488,
0.011946681886911392,
-0.07709090411663055,
0.03617175295948982,
0.046194255352020264,
-0.006776210851967335,
-0.029786137863993645,
-0.09811581671237946,
-0.060134608298540115,
-0.031276095658540726,
-0.022071892395615578,
-0.029238272458314896,
-0.01052927877753973,
0.005727795884013176,
-0.06284572184085846,
0.02043735422194004,
0.0028514349833130836,
-0.06904182583093643,
-0.08328784257173538,
0.02213747426867485,
-0.04840770363807678,
0.08385276794433594,
0.0004391114634927362,
0.0552370548248291,
0.08016151934862137,
0.0039744083769619465,
0.05235433951020241,
-0.01020970568060875,
0.008156509138643742,
-0.01597793586552143,
0.004639860242605209,
0.0037712177727371454,
-0.07846098393201828,
-0.012255688197910786,
-0.027494410052895546,
-0.00004407769665704109,
-0.026079628616571426,
0.014608041383326054,
-0.01095697470009327,
0.013881199061870575,
-0.0018007791368290782,
0.00410826038569212,
0.045539434999227524,
0.0076866429299116135,
-0.029452405869960785,
0.08628368377685547,
-0.022979583591222763,
0.0788312703371048,
0.027935728430747986,
0.054479628801345825,
-0.09304895251989365,
-0.03027338720858097,
-0.024196265265345573,
0.00926673412322998,
0.04703245311975479,
-0.003916206769645214,
0.04705675691366196,
0.015809431672096252,
-0.010788277722895145,
0.0065771667286753654,
0.012208955362439156,
-0.0012170913396403193,
0.05231665074825287,
0.015044782310724258,
-0.05303328111767769,
0.04441332444548607,
-0.07220887392759323,
0.0005847808206453919,
0.05890548974275589,
0.043403059244155884,
-0.28206369280815125,
-0.040613334625959396,
0.06114644929766655,
-0.0021404814906418324,
-0.015356376767158508,
0.042810797691345215,
0.01613903045654297,
-0.016001299023628235,
-0.02124810591340065,
0.07176394015550613,
-0.0399930365383625,
0.011148505844175816,
0.06221012398600578,
0.018658636137843132,
-0.04467777535319328,
-0.01958138309419155,
0.03593671694397926,
-0.07527937740087509,
-0.0024631686974316835,
-0.002043914282694459,
-0.02974858321249485,
-0.06124775856733322,
0.12322990596294403,
-0.04206501320004463,
-0.04710983484983444,
0.008273836225271225,
0.010864865966141224,
0.06882494688034058,
-0.006143141072243452,
-0.06773242354393005,
0.00742643466219306,
-0.03275204449892044,
0.03297341242432594,
-0.035714149475097656,
0.0022331613581627607,
0.05471663922071457,
0.028286514803767204,
-0.00568169541656971,
0.020979948341846466,
0.001593001652508974,
0.0061638373881578445,
-0.008439736440777779,
-0.06120310723781586,
-0.03333631530404091,
0.10333794355392456,
-0.07315809279680252,
-0.009558101184666157,
-0.031661033630371094,
0.0411202572286129,
-0.004750153515487909,
0.0037636025808751583,
0.011896278709173203,
0.010305933654308319,
-0.004848566837608814,
-0.0026653411332517862,
0.000990789383649826,
-0.016395825892686844,
0.0064011807553470135,
-0.03745373338460922,
0.005470277741551399,
0.008125999942421913,
0.003805888583883643,
-0.06247497349977493,
0.04043164476752281,
0.02621772699058056
] |
PMID:27232 | An evaluation of respiration chain-associated functions during initiation of germination of Bacillus megaterium spores. | In Bacillus megaterium QM B1551, spore germination could be initiated by glucose in the absence of detectable oxygen consumption, ATP synthesis or a pH decrease in the external media, suggesting that none of those reactions were mandatory. In addition, initiation of germination was insensitive to a variety of inhibitors of energy production or protonmotive force uncouplers. Therefore the respiratory chain-associated functions are not prerequisites for initiation of germination but these functions may be necessary to drive energy-dependent transport systems and other biosynthetic reactions during outgrowth. | An evaluation of respiration chain-associated functions during initiation of germination of Bacillus megaterium spores. In Bacillus megaterium QM B1551, spore germination could be initiated by glucose in the absence of detectable oxygen consumption, ATP synthesis or a pH decrease in the external media, suggesting that none of those reactions were mandatory. In addition, initiation of germination was insensitive to a variety of inhibitors of energy production or protonmotive force uncouplers. Therefore the respiratory chain-associated functions are not prerequisites for initiation of germination but these functions may be necessary to drive energy-dependent transport systems and other biosynthetic reactions during outgrowth. | [
-0.05630164220929146,
-0.018767638131976128,
0.010344085283577442,
-0.021946139633655548,
0.05457556992769241,
-0.04180213436484337,
0.018875110894441605,
-0.029308924451470375,
-0.01187090203166008,
-0.022896425798535347,
0.02528393641114235,
-0.08048402518033981,
0.02278168313205242,
0.05031950771808624,
-0.0031589011196047068,
-0.05807067081332207,
-0.04105701670050621,
0.05150977522134781,
-0.024462757632136345,
-0.0039047617465257645,
0.04946969449520111,
0.004340974148362875,
0.021361736580729485,
-0.07544400542974472,
0.026978880167007446,
0.020510762929916382,
0.03389045596122742,
0.04502288997173309,
-0.02918202616274357,
-0.1822698414325714,
0.040385302156209946,
-0.011065589264035225,
0.012346436269581318,
-0.03455819562077522,
-0.02802683599293232,
0.05011387914419174,
0.021042652428150177,
0.010573085397481918,
-0.05465658754110336,
0.035126399248838425,
-0.007952066138386726,
0.06295957416296005,
0.012393897399306297,
0.027281109243631363,
0.0159049890935421,
-0.05397321283817291,
-0.0018614395521581173,
0.0026970284525305033,
0.03849736601114273,
-0.0044829812832176685,
-0.047486647963523865,
0.008757367730140686,
-0.0016100778011605144,
0.014980994164943695,
-0.04921917989850044,
-0.04262910783290863,
0.07875660061836243,
0.034084104001522064,
0.042148489505052567,
-0.014836816117167473,
0.03970654681324959,
0.05397656187415123,
-0.1254015415906906,
0.03927300125360489,
0.11033526062965393,
-0.05040987581014633,
-0.032260674983263016,
-0.0517936609685421,
0.057128772139549255,
0.05601830407977104,
-0.03919852524995804,
0.07482616603374481,
-0.010704915970563889,
0.015534582547843456,
0.013835427351295948,
-0.03661021217703819,
-0.025416379794478416,
0.014060905203223228,
0.04909318685531616,
0.006184191908687353,
-0.013472630642354488,
-0.004093129653483629,
0.0025342933367937803,
0.006720485631376505,
-0.0539172999560833,
0.002045378088951111,
0.03723348304629326,
-0.061061445623636246,
-0.03615669533610344,
0.0534900538623333,
0.000016571888409089297,
-0.10656532645225525,
-0.03548234701156616,
0.02100377157330513,
-0.05667205899953842,
0.02010977827012539,
0.012726157903671265,
-0.01682455651462078,
-0.017496971413493156,
0.36526545882225037,
-0.03872263804078102,
0.03426375985145569,
-0.016530940309166908,
0.019542519003152847,
0.0002455410431139171,
-0.02711089327931404,
-0.004034731071442366,
-0.006232084706425667,
-0.019251428544521332,
0.03353403881192207,
0.037577446550130844,
-0.00029894057661294937,
0.02794000133872032,
-0.0321139395236969,
0.04260996729135513,
0.02218889258801937,
0.01822914369404316,
-0.0014460361562669277,
-0.06059522181749344,
0.008327154442667961,
0.035588640719652176,
0.0014813585439696908,
0.04276649281382561,
-0.010284680873155594,
-0.006825433112680912,
0.002816845430061221,
-0.06465750932693481,
0.11111082136631012,
0.014885333366692066,
-0.004896178841590881,
0.0711832344532013,
0.04822744056582451,
-0.004547335673123598,
0.01348143070936203,
-0.03818393871188164,
-0.024126771837472916,
0.019058534875512123,
-0.06950647383928299,
0.0034541133791208267,
-0.023958491161465645,
-0.009303964674472809,
0.0035966974683105946,
-0.011005392298102379,
-0.11636589467525482,
-0.07591323554515839,
0.04902719333767891,
-0.017480751499533653,
0.06013527140021324,
-0.0074165877886116505,
0.024511659517884254,
0.00902029313147068,
0.007760117296129465,
0.038964301347732544,
-0.05227222293615341,
0.011913880705833435,
0.04039842262864113,
-0.030211249366402626,
-0.0005548400804400444,
0.023192718625068665,
-0.04071338102221489,
-0.13006874918937683,
0.026904921978712082,
0.028464708477258682,
0.042320117354393005,
0.03038015216588974,
-0.007256262470036745,
0.012539970688521862,
-0.013906130567193031,
-0.03951448202133179,
-0.01630554348230362,
0.013234744779765606,
0.0010278781410306692,
-0.014907032251358032,
-0.06957917660474777,
0.007330847904086113,
0.000058548583183437586,
0.042528338730335236,
0.016291111707687378,
-0.02132512629032135,
0.04146038740873337,
0.04135901480913162,
-0.036090604960918427,
-0.019433651119470596,
-0.018647121265530586,
0.011443029157817364,
-0.03047657385468483,
-0.07579059153795242,
-0.014750032685697079,
-0.017354045063257217,
0.050430793315172195,
-0.06132075935602188,
-0.008984343148767948,
-0.07412241399288177,
-0.00761721795424819,
-0.020857518538832664,
-0.09127182513475418,
-0.015117112547159195,
0.015924833714962006,
-0.043249715119600296,
-0.022341890260577202,
0.07697715610265732,
-0.011203557252883911,
-0.0361693799495697,
0.02492530830204487,
-0.008116954937577248,
-0.015164078213274479,
0.03845824673771858,
0.05265423282980919,
0.007094583008438349,
-0.04000331088900566,
-0.06031622365117073,
-0.04522893577814102,
-0.03830072283744812,
-0.002514722291380167,
-0.05738906189799309,
0.009283346123993397,
0.003040832234546542,
0.007642367389053106,
0.07644892483949661,
0.016076546162366867,
0.07883494347333908,
-0.009125003591179848,
-0.0569659061729908,
-0.3307994306087494,
0.01604953035712242,
0.013156458735466003,
-0.010366530157625675,
0.06297411024570465,
-0.021673696115612984,
0.06672754138708115,
0.028022227808833122,
-0.038762882351875305,
-0.09690532833337784,
0.014731032773852348,
0.00026844479725696146,
0.010213297791779041,
0.02667764015495777,
0.002259115455672145,
0.04159841313958168,
0.00483021279796958,
-0.06734354048967361,
0.045890163630247116,
0.03922519460320473,
0.056851599365472794,
-0.035539496690034866,
0.041772861033678055,
-0.010245445184409618,
0.0044181798584759235,
-0.03819572553038597,
0.05866760388016701,
0.027458297088742256,
0.04908767342567444,
0.011814177967607975,
0.0548449382185936,
0.052651725709438324,
0.018596043810248375,
-0.060839369893074036,
-0.037736792117357254,
-0.01700560562312603,
0.04405687004327774,
-0.00795804150402546,
0.06521948426961899,
-0.031438082456588745,
-0.054636143147945404,
0.05570061877369881,
-0.06191454082727432,
0.011639979667961597,
-0.006703097373247147,
-0.02479488216340542,
0.010566996410489082,
0.03260181471705437,
-0.02588665671646595,
-0.001045970362611115,
0.04897774010896683,
0.027122454717755318,
0.014403598383069038,
-0.033043790608644485,
0.05914479121565819,
-0.045978717505931854,
0.05899650231003761,
-0.04951929301023483,
-0.03503213822841644,
0.011262375861406326,
-0.004335238132625818,
0.01812170445919037,
-0.03068406879901886,
0.05595819652080536,
-0.02590166963636875,
-0.016746632754802704,
0.006035636644810438,
-0.03307553380727768,
0.0540919154882431,
-0.0553821437060833,
-0.016595900058746338,
0.07417574524879456,
-0.025577256456017494,
-0.0529843345284462,
0.044937267899513245,
0.0162808895111084,
0.000756049354095012,
-0.08317514508962631,
-0.05987648665904999,
-0.05800962448120117,
0.004042459651827812,
-0.017223253846168518,
0.06168186292052269,
-0.034986916929483414,
0.006732918322086334,
0.03799646720290184,
-0.046086836606264114,
-0.03178166225552559,
-0.005733031779527664,
0.03610691428184509,
-0.023202352225780487,
0.0006669031572528183,
0.005702111404389143,
-0.002182729309424758,
0.09750253707170486,
0.008101264014840126,
-0.2735694646835327,
-0.0236202422529459,
0.054015759378671646,
0.04376579821109772,
-0.04448644816875458,
0.037915509194135666,
0.05869666486978531,
-0.021259766072034836,
0.025389403104782104,
-0.022651955485343933,
0.034011248499155045,
-0.05744519457221031,
0.05033804848790169,
0.06426194310188293,
-0.005031841341406107,
0.05523274466395378,
0.03650500252842903,
-0.0522502176463604,
0.005545015912503004,
-0.030124850571155548,
0.006642650347203016,
-0.0022127351257950068,
0.16813445091247559,
0.01009830366820097,
0.02021603286266327,
0.031203579157590866,
0.017845522612333298,
0.039164453744888306,
-0.008201654069125652,
0.018808897584676743,
0.07804397493600845,
-0.011921039782464504,
0.0889708548784256,
-0.0063090757466852665,
0.052187416702508926,
0.0041672661900520325,
0.03893771767616272,
-0.01047342736274004,
0.029631968587636948,
0.007389856968075037,
-0.03163720667362213,
-0.06711556017398834,
0.00810365378856659,
-0.017144130542874336,
0.05382644012570381,
-0.05857296288013458,
-0.007617237512022257,
-0.039675451815128326,
-0.024021008983254433,
0.014240802265703678,
-0.07070016115903854,
-0.025108490139245987,
0.055368032306432724,
0.07129309326410294,
-0.042001914232969284,
0.015386352315545082,
0.017198285087943077,
-0.015010952949523926,
-0.04981376230716705,
0.03761409968137741,
0.04397299140691757,
0.016690753400325775,
-0.033592477440834045,
0.09038103371858597,
-0.018333906307816505
] |
PMID:27233 | Mediated transport and metabolism of lactate in rat aorta. | 1. Under appropriate conditions L- and D-lactate enter the cells of rat aorta and are metabolized. Oxidation of lactate to CO2 occurs under aerobic conditions. 2. L- and D-lactate are taken up into the cells when oxygen, glucose, or both oxygen and glucose are present in the incubation medium. Both L- and D-lactate are excluded from the cells when neither oxygen nor glucose is present. 3. D,L-Glyceraldehyde prevents the uptake of L-lactate. The effect is apparently not due to the inhibition of glucose metabolism by L-glyceraldehyde. 4. L-lactate (20 mM) markedly inhibits the uptake of 5 mM D-lactate, but 20 mM D-lactate fails to inhibit the uptake of 5 mM L-lactate. 5. Raising the pH of the incubation medium markedly depresses the uptake of L-lactate. 6. The results provide evidence that L- and D-lactate enter the cells of rat aorta by a mediated transport system. | Mediated transport and metabolism of lactate in rat aorta. 1. Under appropriate conditions L- and D-lactate enter the cells of rat aorta and are metabolized. Oxidation of lactate to CO2 occurs under aerobic conditions. 2. L- and D-lactate are taken up into the cells when oxygen, glucose, or both oxygen and glucose are present in the incubation medium. Both L- and D-lactate are excluded from the cells when neither oxygen nor glucose is present. 3. D,L-Glyceraldehyde prevents the uptake of L-lactate. The effect is apparently not due to the inhibition of glucose metabolism by L-glyceraldehyde. 4. L-lactate (20 mM) markedly inhibits the uptake of 5 mM D-lactate, but 20 mM D-lactate fails to inhibit the uptake of 5 mM L-lactate. 5. Raising the pH of the incubation medium markedly depresses the uptake of L-lactate. 6. The results provide evidence that L- and D-lactate enter the cells of rat aorta by a mediated transport system. | [
-0.005487833172082901,
-0.0610123872756958,
-0.0015904995379969478,
0.06047957390546799,
0.03274783864617348,
-0.06936822086572647,
0.022852087393403053,
-0.017589254304766655,
-0.056104112416505814,
-0.05600239336490631,
0.051808904856443405,
-0.08808963745832443,
0.044858742505311966,
0.061031196266412735,
-0.02836689166724682,
-0.02867243066430092,
-0.008581442758440971,
-0.025559131056070328,
-0.10270372778177261,
0.005213974043726921,
-0.01982872560620308,
-0.009115288034081459,
0.02515556290745735,
0.01899576745927334,
0.077090784907341,
0.036977581679821014,
0.047164224088191986,
0.007946350611746311,
-0.07462121546268463,
-0.15650060772895813,
-0.022837026044726372,
-0.04172762855887413,
-0.006086064036935568,
-0.018899032846093178,
-0.05582819879055023,
0.0446753054857254,
0.007029360625892878,
0.015091746114194393,
0.01791362464427948,
0.08522038906812668,
0.02388775162398815,
-0.03519822284579277,
0.019342968240380287,
0.05323389172554016,
-0.01529144961386919,
-0.021396871656179428,
-0.05204453319311142,
0.052585504949092865,
0.07018062472343445,
-0.01117877196520567,
-0.03663294389843941,
0.09834782779216766,
0.02895861119031906,
0.004496108740568161,
-0.06480258703231812,
0.004614599980413914,
0.017951976507902145,
-0.029362108558416367,
0.018372826278209686,
0.010452069342136383,
0.03178936615586281,
0.012974150478839874,
-0.13155066967010498,
0.08951792865991592,
0.025741104036569595,
0.005846119485795498,
-0.003800514154136181,
-0.008697761222720146,
0.0763135775923729,
0.07077755033969879,
-0.056698985397815704,
-0.04735453426837921,
-0.005030520260334015,
-0.04749676212668419,
0.05162728205323219,
-0.0448838509619236,
0.03053658828139305,
0.02144370600581169,
0.07459524273872375,
0.03248213976621628,
0.04632486402988434,
-0.008546093478798866,
0.00888312328606844,
-0.011362780816853046,
-0.05003733187913895,
0.0009324630373157561,
0.04557221010327339,
-0.03364704176783562,
-0.05530848354101181,
0.0069071464240550995,
-0.06996115297079086,
-0.0767717957496643,
-0.03995801880955696,
-0.006607087794691324,
-0.05149092897772789,
-0.01255051139742136,
0.03116542100906372,
0.020888684317469597,
0.013243756256997585,
0.35331007838249207,
-0.017711227759718895,
0.034561652690172195,
-0.04081479832530022,
0.0038728166837245226,
-0.04321378096938133,
-0.05583438649773598,
-0.0015153618296608329,
0.049545131623744965,
-0.028801346197724342,
0.02656327374279499,
0.09163085371255875,
0.01662342995405197,
0.011110188439488411,
-0.019863169640302658,
-0.009177782572805882,
0.03627309203147888,
0.03593780845403671,
-0.008543560281395912,
-0.02964433655142784,
0.07399605959653854,
-0.03982127830386162,
0.011385445483028889,
-0.008628923445940018,
-0.022220944985747337,
0.07509905099868774,
-0.02460417151451111,
0.03703983873128891,
0.07737530767917633,
0.08438500761985779,
0.005461864173412323,
0.047791868448257446,
0.0015375674702227116,
-0.022460661828517914,
-0.0222451314330101,
-0.03184026852250099,
-0.028687192127108574,
0.015066390857100487,
-0.07140450179576874,
0.010497611947357655,
-0.028197383508086205,
-0.009414387866854668,
-0.053737085312604904,
-0.01796240545809269,
-0.09378321468830109,
-0.011868742294609547,
0.09700188785791397,
0.03093842975795269,
0.004306009504944086,
-0.04051262140274048,
-0.01353126298636198,
0.025455553084611893,
0.024228239431977272,
0.024001065641641617,
-0.018605010583996773,
-0.019061921164393425,
0.011283659376204014,
-0.05979854241013527,
0.006008285563439131,
0.014414572156965733,
-0.05019237846136093,
-0.032741665840148926,
-0.04823677986860275,
-0.02428331971168518,
0.057094357907772064,
0.006751363165676594,
0.04992486163973808,
-0.02108084224164486,
0.041429050266742706,
0.025759072974324226,
0.0457645058631897,
-0.012510650791227818,
-0.037323854863643646,
-0.024415548890829086,
-0.05586970970034599,
0.004984971601516008,
0.04688481613993645,
0.03663427010178566,
0.03933683782815933,
0.01312015950679779,
-0.013924063183367252,
0.014690175652503967,
-0.04551628977060318,
-0.021545037627220154,
0.015905234962701797,
0.07221189141273499,
0.01679445244371891,
-0.09758716076612473,
-0.020018236711621284,
-0.025675514712929726,
0.07730308920145035,
-0.006892614532262087,
-0.005219377111643553,
-0.11662270873785019,
-0.013840547762811184,
-0.03405885398387909,
-0.017689837142825127,
-0.04144759476184845,
0.016800520941615105,
-0.06065712869167328,
-0.010281435213983059,
0.07420960068702698,
0.015745732933282852,
-0.008210274390876293,
0.014901554211974144,
0.03987717255949974,
0.0006866272306069732,
-0.008952020667493343,
0.02621908113360405,
-0.023102782666683197,
-0.029749944806098938,
-0.03714131563901901,
-0.05093884840607643,
-0.03214867040514946,
0.0031808502972126007,
-0.044275663793087006,
0.02210952341556549,
0.013168072327971458,
-0.04613339528441429,
0.05226224288344383,
0.016618959605693817,
0.043550338596105576,
-0.038471776992082596,
0.027771299704909325,
-0.3082136809825897,
-0.06417588889598846,
0.050051499158144,
0.0667402595281601,
0.041921067982912064,
-0.04954621195793152,
0.042599163949489594,
-0.032179094851017,
-0.027559669688344002,
0.0031243038829416037,
0.037402864545583725,
0.01998159848153591,
-0.0906609296798706,
-0.07131993025541306,
0.02927895076572895,
0.05048762634396553,
-0.018177011981606483,
-0.040373098105192184,
0.04203886538743973,
-0.04042917117476463,
0.04465743899345398,
0.002309463219717145,
0.005565322004258633,
0.0157536081969738,
0.0683780163526535,
-0.005080291535705328,
0.10699519515037537,
-0.019931934773921967,
0.017226843163371086,
-0.04998084530234337,
0.04228263348340988,
0.06854210793972015,
0.030818112194538116,
-0.023097191005945206,
-0.018356312066316605,
-0.07364020496606827,
0.05039088428020477,
-0.017970668151974678,
0.06822934001684189,
-0.04347466304898262,
-0.05823611840605736,
0.010759690776467323,
-0.06472247838973999,
-0.002269760239869356,
-0.021049825474619865,
-0.04161485657095909,
-0.008829493075609207,
0.024370115250349045,
-0.020484820008277893,
-0.06112293526530266,
0.03334977105259895,
0.068531334400177,
0.014229725115001202,
-0.05837583169341087,
0.08070012927055359,
0.041613806039094925,
-0.02129494957625866,
0.04130078852176666,
-0.06158255785703659,
-0.0004869088006671518,
-0.043017227202653885,
0.018873078748583794,
-0.024571998044848442,
-0.011915663257241249,
-0.013329380191862583,
0.04451638087630272,
-0.033600252121686935,
-0.0013382708420976996,
0.0371294766664505,
0.05099738389253616,
0.06200409308075905,
0.09760823100805283,
-0.005686446093022823,
0.07621525973081589,
0.09125881642103195,
0.07933042198419571,
-0.029164185747504234,
-0.002645648317411542,
-0.08131169527769089,
-0.00576917314901948,
0.05584074184298515,
-0.0727316364645958,
0.04886002466082573,
0.029347609728574753,
-0.008716104552149773,
0.008201594464480877,
0.030022770166397095,
-0.03935062140226364,
0.001192794181406498,
-0.026793599128723145,
0.00564682437106967,
0.0011637243442237377,
-0.053626757115125656,
-0.04890020564198494,
0.07215093076229095,
0.017649050801992416,
-0.2236374467611313,
-0.015642382204532623,
0.08366598188877106,
0.0022460022009909153,
-0.002920248545706272,
0.005331309512257576,
-0.00009334080823464319,
-0.029400605708360672,
-0.0045592160895466805,
0.038270819932222366,
0.007741441018879414,
0.03401488810777664,
0.039148032665252686,
0.05934753268957138,
-0.036051686853170395,
0.03721622750163078,
0.03708716854453087,
-0.011637779884040356,
-0.03303951770067215,
-0.057794537395238876,
-0.005084709729999304,
-0.04136088863015175,
0.09392961114645004,
0.022499483078718185,
-0.011157087050378323,
0.03459008038043976,
-0.009452968835830688,
0.058638691902160645,
-0.025731069967150688,
0.05976206064224243,
-0.03766802325844765,
0.030078593641519547,
0.059108585119247437,
-0.11936073750257492,
0.02207775227725506,
0.027698783203959465,
-0.0023416937328875065,
0.024117032065987587,
-0.02208901196718216,
0.007432454265654087,
-0.016329148784279823,
-0.007903430610895157,
-0.0693761333823204,
0.024533623829483986,
0.053639546036720276,
-0.01469939574599266,
-0.01947476714849472,
-0.029588863253593445,
0.003646235214546323,
0.009799327701330185,
0.022356664761900902,
-0.02235783264040947,
0.031548913568258286,
0.02831038273870945,
0.02658439427614212,
-0.052481528371572495,
-0.01394124235957861,
-0.05732472613453865,
-0.048665162175893784,
-0.004551100544631481,
0.008921320550143719,
-0.0350111648440361,
-0.01221734844148159,
0.11120989173650742,
0.011795301921665668
] |
PMID:27234 | Changes in collagen cross-linking and lysyl oxidase by estrogen. | Dermal collagen solubility and lysyl oxidase activity of bones were measured in DDD mice of advancing age. Insoluble fractions of the dermal collagen increased more rapidly in females than in males after 5 weeks of age. Activity of the lysyl oxidase extracted from bones was higher in females than in males after 4 weeks of age. After sexual maturation, such sex differences were always observed in skin as well as in bone tissue. In other experimental animals, dermal collagen solubility was markedly decreased by estrogen treatment and lysyl oxidase was remarkably activated by estrogen in both skin and bone. Thus it is clear that estrogen stimulates the enzyme activity and accelerates the maturation of collagen and elastin in extracellular space. | Changes in collagen cross-linking and lysyl oxidase by estrogen. Dermal collagen solubility and lysyl oxidase activity of bones were measured in DDD mice of advancing age. Insoluble fractions of the dermal collagen increased more rapidly in females than in males after 5 weeks of age. Activity of the lysyl oxidase extracted from bones was higher in females than in males after 4 weeks of age. After sexual maturation, such sex differences were always observed in skin as well as in bone tissue. In other experimental animals, dermal collagen solubility was markedly decreased by estrogen treatment and lysyl oxidase was remarkably activated by estrogen in both skin and bone. Thus it is clear that estrogen stimulates the enzyme activity and accelerates the maturation of collagen and elastin in extracellular space. | [
-0.013198020868003368,
-0.06489496678113937,
0.012167343869805336,
0.0514141321182251,
0.04725930094718933,
-0.04260183125734329,
0.004750759806483984,
0.01467203814536333,
-0.00781960878521204,
-0.05708823725581169,
-0.001277413684874773,
-0.05295460298657417,
0.004586691968142986,
0.015680929645895958,
-0.015476342290639877,
-0.007086971774697304,
0.01725354976952076,
0.02345867082476616,
-0.09108579158782959,
0.020997200161218643,
0.008100327104330063,
0.01590118743479252,
0.04672848805785179,
-0.03992797061800957,
0.03119817189872265,
0.011269020847976208,
-0.03200529143214226,
-0.07369168102741241,
-0.08182688057422638,
-0.16347001492977142,
0.0395011305809021,
-0.030516384169459343,
0.03638644516468048,
-0.023596445098519325,
-0.00902869924902916,
0.04066578671336174,
-0.05050904303789139,
-0.006364042405039072,
0.02928551845252514,
0.008889018557965755,
0.06479455530643463,
0.0040022823959589005,
-0.04918030649423599,
0.027772434055805206,
0.04256346821784973,
0.021879198029637337,
-0.05331139639019966,
0.037334199994802475,
0.017383230850100517,
0.019434304907917976,
0.006150048691779375,
-0.005092986393719912,
0.04803754761815071,
0.0333993025124073,
0.026915831491351128,
0.04412154480814934,
0.02171958051621914,
0.05719783902168274,
-0.025556163862347603,
0.07422471791505814,
0.00009587569365976378,
-0.021216539666056633,
-0.1466342657804489,
0.06521736830472946,
0.0658220499753952,
0.004258894827216864,
0.030823592096567154,
-0.03210393339395523,
0.0815793052315712,
0.011838828213512897,
-0.03685309737920761,
0.08212127536535263,
-0.011914747767150402,
0.007286406122148037,
0.10214915126562119,
0.007593201939016581,
-0.004873845726251602,
-0.027570772916078568,
0.06393713504076004,
0.027174757793545723,
-0.030822578817605972,
0.024396385997533798,
0.024214763194322586,
-0.028228236362338066,
0.0006167074316181242,
-0.0233782809227705,
-0.014833628199994564,
-0.0756269097328186,
-0.018478790298104286,
0.04358752816915512,
-0.07054638862609863,
-0.02281782776117325,
-0.027448590844869614,
0.005056349094957113,
-0.034190330654382706,
-0.0020741880871355534,
0.07752019912004471,
0.04000319167971611,
0.008695488795638084,
0.37615111470222473,
-0.030844347551465034,
0.062163397669792175,
-0.04801330342888832,
-0.007737711071968079,
0.008943977765738964,
-0.04865269362926483,
-0.01761619746685028,
-0.020528588443994522,
-0.049762994050979614,
0.06491576880216599,
0.038166120648384094,
0.01239524595439434,
-0.03808224946260452,
-0.05345028266310692,
-0.03163183107972145,
-0.04494037106633186,
0.013601009733974934,
0.024316243827342987,
-0.011826987378299236,
0.02795746549963951,
-0.03318895399570465,
-0.029037432745099068,
0.009709438309073448,
-0.03484833240509033,
-0.0115674352273345,
0.014489051885902882,
0.00920760165899992,
0.03992908075451851,
0.05532648786902428,
0.020134204998612404,
-0.02209714613854885,
-0.038360677659511566,
-0.04445671662688255,
0.042784683406353,
-0.020558346062898636,
-0.02121679112315178,
-0.025694679468870163,
-0.003807635046541691,
-0.021087147295475006,
-0.015214246697723866,
-0.04443948715925217,
-0.11859837919473648,
0.00904889591038227,
-0.056881554424762726,
0.012074662372469902,
0.10891352593898773,
0.06046073138713837,
0.01224287785589695,
0.043819528073072433,
0.019439300522208214,
0.05638636648654938,
0.07832599431276321,
-0.0808793231844902,
0.05297220125794411,
-0.0351041704416275,
0.002973655005916953,
0.012045701034367085,
-0.06947683542966843,
0.041964657604694366,
-0.036577627062797546,
-0.0164395309984684,
-0.03810453042387962,
-0.05255111679434776,
-0.013697466813027859,
0.07293582707643509,
-0.01711800880730152,
-0.004750854801386595,
0.09351597726345062,
0.03021743893623352,
-0.011049936525523663,
0.04722799360752106,
-0.020276110619306564,
-0.061854105442762375,
-0.03582040220499039,
-0.013135367073118687,
0.025275999680161476,
0.03783416748046875,
-0.0011369285639375448,
0.0032965047284960747,
0.019544290378689766,
0.03907144442200661,
-0.023369520902633667,
-0.06725083291530609,
0.050810929387807846,
0.04410118609666824,
-0.058611731976270676,
-0.00437130406498909,
0.01213857065886259,
0.002488149330019951,
-0.007332715671509504,
0.006766635458916426,
-0.013752252794802189,
-0.05663897469639778,
0.007813370786607265,
-0.01834862492978573,
-0.0187002494931221,
-0.039489250630140305,
0.015884727239608765,
-0.023291882127523422,
0.007344863377511501,
0.0644296407699585,
0.009209650568664074,
0.019350433722138405,
0.016904519870877266,
-0.011539552360773087,
0.06676362454891205,
0.006199972238391638,
-0.00959959626197815,
0.06176544353365898,
-0.008399991318583488,
-0.04592550918459892,
0.027189522981643677,
0.028402043506503105,
-0.016019457951188087,
-0.05173655226826668,
0.057128436863422394,
0.039356354624032974,
-0.0628923550248146,
0.050607964396476746,
0.008951338939368725,
0.03665870055556297,
-0.04759489744901657,
-0.03192392364144325,
-0.3050760328769684,
-0.006535313092172146,
0.07006051391363144,
-0.03377577289938927,
0.023409046232700348,
-0.011922979727387428,
0.011284871026873589,
-0.08225757628679276,
0.02059355564415455,
0.009435039013624191,
0.08295480906963348,
0.1012946367263794,
-0.024776212871074677,
-0.05147641524672508,
0.047836314886808395,
0.034894052892923355,
0.06315898150205612,
-0.03140288591384888,
0.021536899730563164,
-0.019296273589134216,
0.025208821520209312,
-0.021362556144595146,
0.02668202854692936,
-0.004486431833356619,
0.0411248654127121,
-0.014869733713567257,
0.10116080939769745,
0.009170196950435638,
-0.06093919649720192,
0.00870607141405344,
-0.006242998410016298,
-0.006625025998800993,
-0.03797328099608421,
-0.12664645910263062,
-0.020618997514247894,
-0.03369992971420288,
0.012528127990663052,
-0.06888647377490997,
-0.01481787022203207,
0.01710798218846321,
-0.07013777643442154,
0.016623331233859062,
0.021285373717546463,
-0.015348292887210846,
-0.045259155333042145,
-0.032673634588718414,
0.03824083134531975,
0.004673117771744728,
0.00168123678304255,
-0.024899331852793694,
0.03374316915869713,
-0.0026850197464227676,
0.035006701946258545,
0.024927815422415733,
-0.0065516941249370575,
0.00958063080906868,
-0.035554416477680206,
0.010528765618801117,
-0.028826940804719925,
-0.04700301215052605,
-0.030639085918664932,
0.004826554097235203,
-0.014984944835305214,
-0.05295133963227272,
0.010043831542134285,
-0.013478566892445087,
0.00016170491289813071,
-0.09205232560634613,
0.016663778573274612,
-0.02728467620909214,
0.04086093604564667,
0.08329053968191147,
-0.029338452965021133,
-0.02739640511572361,
0.07400569319725037,
0.03811383247375488,
-0.0788174569606781,
0.0015262516681104898,
-0.0648701936006546,
0.02724086493253708,
0.021070316433906555,
-0.060744959861040115,
0.03334873914718628,
0.022859349846839905,
0.03754638880491257,
-0.04767317697405815,
0.002136454451829195,
0.004066685680299997,
0.0011961159761995077,
0.044174592941999435,
0.01634538546204567,
-0.000995276728644967,
-0.014914852567017078,
-0.028416553512215614,
-0.016967052593827248,
-0.06242544203996658,
-0.27003854513168335,
0.036203864961862564,
0.06084698811173439,
-0.014314590021967888,
-0.07310348004102707,
-0.007631619926542044,
0.00688796304166317,
-0.026254238560795784,
-0.001480126054957509,
0.0885576531291008,
0.0015685391845181584,
0.02340472675859928,
0.028972743079066277,
0.0077253892086446285,
-0.020462974905967712,
0.023316295817494392,
0.007559909950941801,
0.011526064947247505,
0.0254988856613636,
-0.0554356649518013,
0.020598161965608597,
-0.0783480629324913,
0.11426689475774765,
0.024499215185642242,
0.005600025411695242,
0.028537476435303688,
-0.020042354241013527,
-0.007174648344516754,
0.00666583189740777,
-0.012803545221686363,
0.0015127628576010466,
0.07635177671909332,
0.021753014996647835,
0.00263585546053946,
0.008333266712725163,
0.11398071050643921,
-0.011811306700110435,
0.03860267996788025,
0.0218322966247797,
0.00869224313646555,
0.03735698014497757,
-0.03216579556465149,
-0.0635865330696106,
0.03340727835893631,
0.07458141446113586,
-0.11388447135686874,
0.01729544624686241,
-0.012294426560401917,
0.03878958523273468,
-0.01313349511474371,
-0.0022996508050709963,
-0.045424941927194595,
0.04325328767299652,
0.02315208502113819,
-0.017144624143838882,
-0.0415823720395565,
-0.051598768681287766,
0.01669669896364212,
-0.008409108966588974,
0.0034891602117568254,
0.04004873335361481,
-0.009373465552926064,
-0.08757131546735764,
0.09252582490444183,
0.050999999046325684
] |
PMID:27235 | Effects of catecholamines and glucagon on glycogen metabolism in human polymorphonuclear leukocytes. | Addition of 10 micron of the alpha-adrenergic agonist phenylephrine to polymorphonuclear leukocytes suspended in glucose-free Krebs-Ringer bicarbonate buffer (pH 6.7) activated phosphorylase, inactivated glycogen synthase R maximally within 30 s, and resulted in glycogen breakdown. Phenylephrine increased 45Ca efflux relative to control of 45Ca prelabelled cells, but did not affect cyclic adenosine 3',5'-monophosphate (cAMP) concentration. The effects of phenylephrine were blocked by 20 micron phentolamine and were absent in cells incubated at pH 7.4. The same unexplained dependency of extracellular pH was observed with 2.5 nM--2.5 micron glucagon, which activated phosphorylase and inactivated synthase-R, but in addition caused a 30-s burst in cAMP formation. 25 nM glucagon also increased 45Ca efflux. The activation of phosphorylase by phenylephrine and possibly also by glucagon are thought mediated by an increased concentration of cytosolic Ca2+ activating phosphorylase kinase. The effects of 5 micron isoproterenol or 5 micron epinephrine were independent of extracellular pH 6.7 and 7.4 and resulted in a sustained increase in cAMP, an activation of phosphorylase and inactivation of synthase-R within 15 s, and in glycogenolysis. The effects of both compounds were blocked by 10 micron propranolol, whereas 10 micron phentolamine had no effect on the epinephrine action. The efflux of 45Ca was not affected by either isoproterenol or epinephrine. The beta-adrenergic activation of phosphorylase is consistent with the assumption of a covalent modification of phosphorylase kinase by the cAMP dependent protein kinase. Phosphorylation of synthase-R to synthase-D can thus occur independently of increase in cAMP, but the evidence is inconclusive with respect to the cAMP dependent protein kinase also being active in this phosphorylation. | Effects of catecholamines and glucagon on glycogen metabolism in human polymorphonuclear leukocytes. Addition of 10 micron of the alpha-adrenergic agonist phenylephrine to polymorphonuclear leukocytes suspended in glucose-free Krebs-Ringer bicarbonate buffer (pH 6.7) activated phosphorylase, inactivated glycogen synthase R maximally within 30 s, and resulted in glycogen breakdown. Phenylephrine increased 45Ca efflux relative to control of 45Ca prelabelled cells, but did not affect cyclic adenosine 3',5'-monophosphate (cAMP) concentration. The effects of phenylephrine were blocked by 20 micron phentolamine and were absent in cells incubated at pH 7.4. The same unexplained dependency of extracellular pH was observed with 2.5 nM--2.5 micron glucagon, which activated phosphorylase and inactivated synthase-R, but in addition caused a 30-s burst in cAMP formation. 25 nM glucagon also increased 45Ca efflux. The activation of phosphorylase by phenylephrine and possibly also by glucagon are thought mediated by an increased concentration of cytosolic Ca2+ activating phosphorylase kinase. The effects of 5 micron isoproterenol or 5 micron epinephrine were independent of extracellular pH 6.7 and 7.4 and resulted in a sustained increase in cAMP, an activation of phosphorylase and inactivation of synthase-R within 15 s, and in glycogenolysis. The effects of both compounds were blocked by 10 micron propranolol, whereas 10 micron phentolamine had no effect on the epinephrine action. The efflux of 45Ca was not affected by either isoproterenol or epinephrine. The beta-adrenergic activation of phosphorylase is consistent with the assumption of a covalent modification of phosphorylase kinase by the cAMP dependent protein kinase. Phosphorylation of synthase-R to synthase-D can thus occur independently of increase in cAMP, but the evidence is inconclusive with respect to the cAMP dependent protein kinase also being active in this phosphorylation. | [
-0.015684522688388824,
-0.05046888068318367,
0.00616411492228508,
0.043374087661504745,
-0.023080455139279366,
-0.048082318156957626,
0.04512792080640793,
0.015281466767191887,
-0.04513511434197426,
-0.04083869233727455,
-0.010069504380226135,
-0.041537873446941376,
-0.03416293486952782,
0.08698009699583054,
-0.011233395896852016,
-0.031284868717193604,
-0.011295739561319351,
-0.0030067148618400097,
-0.1089835986495018,
-0.02041647583246231,
-0.009743226692080498,
-0.002533091465011239,
0.04186831787228584,
0.00950322300195694,
0.028912924230098724,
-0.014106822200119495,
0.012149505317211151,
0.03347821161150932,
-0.06830242276191711,
-0.1730751097202301,
-0.015328834764659405,
-0.007406854536384344,
0.05382480099797249,
-0.03178229182958603,
-0.047624994069337845,
0.010588466189801693,
-0.029788481071591377,
0.02559634856879711,
0.008005689829587936,
0.10197222232818604,
-0.022280680015683174,
0.008558236993849277,
-0.021035322919487953,
0.051286667585372925,
0.04596955329179764,
0.002737589878961444,
-0.048856012523174286,
-0.039175231009721756,
0.09950075298547745,
0.02215828001499176,
0.011982419528067112,
-0.0016571172745898366,
0.020265480503439903,
0.0465371310710907,
0.0036190517712384462,
-0.004053759388625622,
0.009416176937520504,
0.03227363899350166,
0.027118660509586334,
0.012722243554890156,
0.0077643790282309055,
0.0628664642572403,
-0.1811935007572174,
0.041680365800857544,
0.05228037387132645,
-0.02481091581285,
-0.02902182750403881,
-0.01487181056290865,
0.03733181208372116,
0.07471449673175812,
-0.0765339583158493,
0.05366424471139908,
0.01716933585703373,
-0.0577409528195858,
-0.009683262556791306,
0.00441167876124382,
-0.0019978643395006657,
0.007389540784060955,
0.03414643928408623,
0.033862754702568054,
0.04737165942788124,
0.0006153623689897358,
0.009359080344438553,
-0.03926125168800354,
0.012080959975719452,
0.0024287269916385412,
-0.02166622132062912,
-0.010315150022506714,
-0.005540636368095875,
-0.010457843542098999,
-0.040055640041828156,
-0.05457977205514908,
0.003933596890419722,
0.005862127523869276,
-0.07075446844100952,
0.039067380130290985,
0.03429943323135376,
0.007240590639412403,
-0.015549981035292149,
0.4080818295478821,
0.0009309023153036833,
0.03357631340622902,
-0.07092662155628204,
0.022857004776597023,
0.032142553478479385,
-0.06680898368358612,
-0.017390072345733643,
-0.010834024287760258,
-0.0038019861094653606,
0.057152461260557175,
0.08677897602319717,
0.014369646087288857,
0.004828958306461573,
-0.008749186992645264,
-0.0033301652874797583,
-0.04490074887871742,
0.07601432502269745,
0.01811330020427704,
-0.021484116092324257,
0.07237009704113007,
-0.012437007389962673,
-0.023136446252465248,
-0.009091065265238285,
0.012390515767037868,
0.07981215417385101,
0.002512476174160838,
-0.03317420184612274,
0.09724704921245575,
0.05374791845679283,
-0.03182051703333855,
0.0729212611913681,
0.04875485226511955,
-0.014532692730426788,
0.060889843851327896,
-0.0328344888985157,
-0.018097830936312675,
0.06275877356529236,
-0.006719714961946011,
0.03361967206001282,
0.05364370718598366,
0.00872198399156332,
-0.0371243879199028,
-0.025153441354632378,
-0.08958736062049866,
-0.028921646997332573,
0.12924881279468536,
0.060346897691488266,
0.03716493025422096,
-0.05543980374932289,
0.0031928452663123608,
0.022326456382870674,
0.04585689678788185,
-0.010486969724297523,
-0.007966721430420876,
-0.04972939193248749,
0.08349427580833435,
-0.026507003232836723,
-0.008816564455628395,
0.03315221890807152,
-0.01639888808131218,
-0.042992670089006424,
0.01851755380630493,
0.01735677570104599,
0.006791241932660341,
0.05957987532019615,
0.010188937187194824,
-0.023911628872156143,
-0.012855333276093006,
0.0019340261351317167,
-0.0201365128159523,
0.003050520783290267,
0.009041201323270798,
-0.022458519786596298,
-0.01879418082535267,
-0.008353269658982754,
0.0015354665229097009,
0.019380902871489525,
0.026619803160429,
0.028158321976661682,
-0.02947199158370495,
-0.004243385512381792,
-0.015122656710445881,
0.0061107720248401165,
0.014190230518579483,
0.020001443102955818,
-0.03893827646970749,
-0.0012470221845433116,
-0.013270922005176544,
-0.0025307543110102415,
0.06256821751594543,
0.002449118997901678,
-0.0051695299334824085,
-0.08050508052110672,
-0.02652885764837265,
-0.04002394154667854,
-0.04711636155843735,
-0.04671700298786163,
-0.004327688831835985,
-0.04295152425765991,
-0.020501095801591873,
0.08748546242713928,
0.014012408442795277,
0.007249572779983282,
0.05278588831424713,
-0.018149634823203087,
0.03858723118901253,
-0.05656438320875168,
0.034217193722724915,
0.06149055063724518,
0.021188069134950638,
-0.015626240521669388,
0.015217931009829044,
-0.005517836194485426,
-0.0019931441638618708,
-0.04196576029062271,
0.03243144974112511,
-0.03641602024435997,
0.011907310225069523,
0.04751960560679436,
-0.05702963471412659,
0.03470885753631592,
-0.022023862227797508,
-0.020139727741479874,
-0.3095608055591583,
-0.02142411842942238,
0.04987113177776337,
0.0017014873446896672,
-0.001548525644466281,
-0.042052172124385834,
0.0804654210805893,
-0.03914472088217735,
0.0012461175210773945,
-0.06650476157665253,
0.037898339331150055,
0.06398947536945343,
-0.035269685089588165,
-0.007872379384934902,
0.02501610293984413,
0.06904575228691101,
0.016857683658599854,
-0.059765588492155075,
0.03834371641278267,
-0.00407429737970233,
0.030626023188233376,
0.01155420858412981,
0.024872062727808952,
0.0011025508865714073,
0.03850569576025009,
-0.056274931877851486,
0.0959480032324791,
0.0009685013792477548,
0.0019758502021431923,
-0.023235023021697998,
0.03520306944847107,
0.06622631102800369,
-0.0033318502828478813,
-0.10110638290643692,
0.007472774013876915,
-0.002940158126875758,
0.00700950575992465,
0.006046595051884651,
0.002122999634593725,
-0.02306828647851944,
-0.06160816177725792,
0.013474754057824612,
-0.04375448822975159,
-0.06282822787761688,
-0.011128455400466919,
-0.006427668035030365,
-0.0018700264627113938,
0.05606712028384209,
-0.04498668760061264,
-0.004426233936101198,
0.03352857008576393,
0.018048394471406937,
0.02116057649254799,
-0.05436936020851135,
0.039484456181526184,
-0.022144628688693047,
-0.051469240337610245,
0.06448701024055481,
-0.07487331330776215,
0.00595106789842248,
-0.030318213626742363,
-0.0006123082130216062,
-0.003759774612262845,
0.03548649698495865,
-0.015025508590042591,
0.01841171458363533,
-0.01587998867034912,
-0.024645932018756866,
0.03596806526184082,
0.0290520042181015,
0.05672669783234596,
0.09632430970668793,
-0.01652032509446144,
0.015042093582451344,
0.04110383987426758,
0.004546687472611666,
-0.04163690283894539,
-0.038286469876766205,
-0.10964291542768478,
-0.005477097351104021,
-0.0015256537590175867,
-0.06923206895589828,
0.08286449313163757,
-0.025458814576268196,
-0.04824947938323021,
0.01060433778911829,
0.01199196744710207,
0.018933679908514023,
0.05052108317613602,
-0.004291990771889687,
-0.0567387193441391,
-0.0025518329348415136,
-0.03819625452160835,
-0.06612736731767654,
0.06674382835626602,
0.020140670239925385,
-0.2616109848022461,
0.013479410670697689,
-0.015125895850360394,
-0.05354528874158859,
-0.0336332805454731,
0.03774069622159004,
0.005599423311650753,
-0.03489549830555916,
-0.057866696268320084,
0.026845350861549377,
0.042442500591278076,
0.023221947252750397,
0.08714505285024643,
0.029430963099002838,
-0.051178205758333206,
0.026965539902448654,
0.04952172562479973,
-0.04702340066432953,
-0.002540335990488529,
-0.00909673422574997,
-0.027492331340909004,
-0.05703423172235489,
0.13082252442836761,
-0.020753564313054085,
-0.02651858702301979,
0.020656103268265724,
-0.025787383317947388,
0.004275282844901085,
0.0012391249183565378,
-0.002463909098878503,
-0.059312254190444946,
0.0419083908200264,
0.05878157541155815,
-0.028851110488176346,
0.02518102340400219,
0.03574568033218384,
-0.0008545490563847125,
-0.0007516429759562016,
-0.03759868070483208,
-0.005773298442363739,
0.016534939408302307,
0.026818927377462387,
0.0033003012649714947,
-0.04589512571692467,
0.04678242281079292,
-0.042078569531440735,
0.025765154510736465,
-0.07508847862482071,
-0.01932474784553051,
0.0035998334642499685,
-0.049661699682474136,
-0.04225572571158409,
-0.013428687117993832,
0.07411802560091019,
-0.023979634046554565,
-0.018755106255412102,
-0.02467232011258602,
-0.028878867626190186,
0.01882736198604107,
-0.0175899937748909,
-0.006368314381688833,
-0.007781557738780975,
-0.045227374881505966,
0.07026254385709763,
0.022260604426264763
] |
PMID:27236 | Properties and partial purification of the detergent-solubilized insulin receptor: a demonstration of negative cooperativity in micellar solution. | Turkey erythrocytes possess insulin receptors with binding properties very similar to those of mammalian insulin receptors. In the present study, the insulin receptor of the avian erythrocyte has been solubilized in Triton X-100, extensively characterized and partially purified, and its properties compared to those of the membrane-bound receptor. The solubilized insulin receptor has a Stokes radius of 70 A and an apparent molecular weight of 300 000 in 0.05% Triton. The binding of insulin to the soluble receptor was very similar to the binding observed with the membrane-bound receptor. Thus, binding was markedly temperature dependent for both the soluble and membrane-bound forms, although the kinetics of binding were slower with the soluble receptor. Both forms of the receptor also showed a sharp pH optimum; however, solubilization produced a shift from maximal binding at pH 7.8 to pH 7.3. The soluble receptor also retained insulin analog specificity, ion sensitivity and negative cooperativity. The soluble receptor did not appear to degrade either bound or free insulin. On DEAE-cellulose chromatography the receptor eluted as a single peak. The specific activity of this partially purified preparation was 25--30 pmol/mg protein (about 500-fold enrichment over crude extract and 5-fold over highly purified membranes). Extensive attempts to purify further the receptor by gel filtration, carboxymethyl-cellulose chromatography and affinity chromatography resulted in either a very low yield or only modest enrichment. Purification was also complicated because the receptor was easily denatured; about 40% of the activity was lost after a 90-min exposure to 3 M urea or pH 4.5. These data suggest that the insulin receptor retains its properties in the absence of the lipid bilayer of the membrane. Complete purification will be difficult due to a lack of stability under a number of conditions. | Properties and partial purification of the detergent-solubilized insulin receptor: a demonstration of negative cooperativity in micellar solution. Turkey erythrocytes possess insulin receptors with binding properties very similar to those of mammalian insulin receptors. In the present study, the insulin receptor of the avian erythrocyte has been solubilized in Triton X-100, extensively characterized and partially purified, and its properties compared to those of the membrane-bound receptor. The solubilized insulin receptor has a Stokes radius of 70 A and an apparent molecular weight of 300 000 in 0.05% Triton. The binding of insulin to the soluble receptor was very similar to the binding observed with the membrane-bound receptor. Thus, binding was markedly temperature dependent for both the soluble and membrane-bound forms, although the kinetics of binding were slower with the soluble receptor. Both forms of the receptor also showed a sharp pH optimum; however, solubilization produced a shift from maximal binding at pH 7.8 to pH 7.3. The soluble receptor also retained insulin analog specificity, ion sensitivity and negative cooperativity. The soluble receptor did not appear to degrade either bound or free insulin. On DEAE-cellulose chromatography the receptor eluted as a single peak. The specific activity of this partially purified preparation was 25--30 pmol/mg protein (about 500-fold enrichment over crude extract and 5-fold over highly purified membranes). Extensive attempts to purify further the receptor by gel filtration, carboxymethyl-cellulose chromatography and affinity chromatography resulted in either a very low yield or only modest enrichment. Purification was also complicated because the receptor was easily denatured; about 40% of the activity was lost after a 90-min exposure to 3 M urea or pH 4.5. These data suggest that the insulin receptor retains its properties in the absence of the lipid bilayer of the membrane. Complete purification will be difficult due to a lack of stability under a number of conditions. | [
-0.06427870690822601,
-0.036622609943151474,
-0.036498669534921646,
0.021246571093797684,
-0.009011030197143555,
-0.0511663518846035,
0.06545395404100418,
0.031532108783721924,
-0.06741549074649811,
0.030185848474502563,
-0.03761390224099159,
-0.10626757144927979,
0.0026880200020968914,
0.02397054061293602,
0.0007556611089967191,
-0.030020512640476227,
-0.02009190060198307,
-0.014201756566762924,
-0.056281622499227524,
0.0056745936162769794,
-0.00940428301692009,
-0.1066306084394455,
0.008970578201115131,
-0.015079230070114136,
0.05525107681751251,
0.029781529679894447,
-0.06051221862435341,
-0.00867912545800209,
-0.0447356179356575,
-0.19768433272838593,
0.038290977478027344,
-0.02855428121984005,
0.016879750415682793,
-0.033735644072294235,
-0.04467818886041641,
0.001052957959473133,
-0.029728703200817108,
-0.002036839257925749,
-0.056051068007946014,
0.01481567695736885,
0.06106339022517204,
-0.005588199477642775,
-0.0034438648726791143,
0.03968403860926628,
0.03537121042609215,
-0.006250421050935984,
-0.04740411415696144,
-0.01943935640156269,
0.076047882437706,
-0.01078737247735262,
0.00773070752620697,
-0.014235671609640121,
0.03219076618552208,
0.0374460369348526,
0.0040844157338142395,
0.02244342677295208,
-0.02150164358317852,
0.05248737707734108,
0.028856046497821808,
-0.02520179934799671,
-0.023001858964562416,
0.027748020365834236,
-0.077826127409935,
0.0505693294107914,
0.05702236294746399,
0.01477737259119749,
-0.00608172407373786,
-0.017191018909215927,
0.04191282019019127,
0.044930070638656616,
-0.015456389635801315,
0.05857856199145317,
-0.011227387934923172,
0.009058447554707527,
-0.002892240881919861,
-0.028593873605132103,
0.025787197053432465,
0.04969247803092003,
0.04037797823548317,
0.04812884330749512,
-0.022612635046243668,
0.005168399307876825,
0.001667053671553731,
-0.030293231830000877,
-0.0369214229285717,
0.07168253511190414,
0.0037759935948997736,
-0.03855019062757492,
0.012926103547215462,
-0.005277703516185284,
-0.041173312813043594,
-0.011563040316104889,
0.004116728436201811,
-0.01102972961962223,
-0.1016642153263092,
0.03412219136953354,
-0.020670834928750992,
-0.02682318724691868,
-0.03043372370302677,
0.3910641670227051,
-0.012428568676114082,
0.03989025205373764,
-0.03178893029689789,
-0.015852469950914383,
0.00641778064891696,
-0.07127445191144943,
-0.005859905853867531,
-0.0032318569719791412,
-0.01953216642141342,
0.03787528723478317,
-0.029515406116843224,
-0.008927572518587112,
0.026628192514181137,
-0.016237007454037666,
0.033441781997680664,
-0.024149786680936813,
-0.006625530309975147,
-0.0000923800325836055,
0.04095907136797905,
0.030474986881017685,
-0.062014300376176834,
-0.028994692489504814,
0.0034494707360863686,
0.026694701984524727,
0.019390715286135674,
-0.010129282251000404,
-0.06913315504789352,
0.08628406375646591,
0.05685175210237503,
0.018439173698425293,
0.057860177010297775,
0.03963654115796089,
-0.05755721777677536,
0.04262293875217438,
0.01743965595960617,
-0.010054502636194229,
0.005043988116085529,
-0.029794832691550255,
0.011867162771522999,
-0.0455491840839386,
-0.03327713906764984,
-0.01752917654812336,
-0.015406562015414238,
-0.052365802228450775,
-0.02085208334028721,
0.08889856934547424,
0.02073211781680584,
0.00985942967236042,
-0.03538788855075836,
0.029199354350566864,
0.02855386584997177,
0.055338140577077866,
0.004769810475409031,
0.03250770643353462,
0.049058690667152405,
0.0519835501909256,
-0.01018856093287468,
-0.01821483112871647,
0.004482252523303032,
-0.006104929372668266,
-0.031015098094940186,
-0.023643217980861664,
0.013919624499976635,
0.040952038019895554,
-0.004306917078793049,
-0.0076162926852703094,
-0.021068984642624855,
0.003519853577017784,
0.0035420653875917196,
0.047276485711336136,
0.028589939698576927,
-0.05273331329226494,
0.00013554573524743319,
-0.023958859965205193,
-0.024505553767085075,
0.027534758672118187,
0.003338515292853117,
0.013541082851588726,
0.013218354433774948,
-0.04223131760954857,
-0.009449572302401066,
-0.002297573257237673,
0.0022988496348261833,
0.06953182071447372,
0.044334229081869125,
-0.04033869877457619,
-0.08641701191663742,
-0.014311700128018856,
-0.010195382870733738,
0.06673869490623474,
-0.08835820853710175,
0.034468770027160645,
-0.08494805544614792,
0.014271167106926441,
-0.022265076637268066,
-0.017220309004187584,
0.0073929522186517715,
-0.02447224035859108,
-0.07288699597120285,
-0.006067574489861727,
0.1290237009525299,
0.033543139696121216,
-0.0017061897087842226,
0.0969204530119896,
0.02648964896798134,
0.10127343237400055,
0.043574728071689606,
-0.05250714719295502,
0.038410820066928864,
0.039672818034887314,
0.03259051963686943,
0.0016495056916028261,
-0.07171124964952469,
0.00032059013028629124,
-0.05367288365960121,
0.03158828988671303,
0.06639761477708817,
0.0003007490886375308,
0.04980902001261711,
0.038902733474969864,
0.07469411194324493,
-0.03678485006093979,
-0.05345020443201065,
-0.304777055978775,
-0.007439205888658762,
-0.010109459049999714,
-0.01391199603676796,
0.063339002430439,
-0.03268454596400261,
0.00487247109413147,
-0.004381414968520403,
0.05066099762916565,
-0.06823095679283142,
0.04555239528417587,
0.05154886096715927,
0.005027053877711296,
-0.024678193032741547,
0.029923316091299057,
0.07476846128702164,
0.02436858043074608,
0.035322703421115875,
0.045657481998205185,
0.0004711092624347657,
-0.005916681606322527,
0.02825479581952095,
0.029092716053128242,
0.04945052042603493,
0.02856426127254963,
-0.04525231942534447,
0.10309237986803055,
0.013581721112132072,
-0.005359363276511431,
0.04445645958185196,
-0.0021654933225363493,
0.0236922986805439,
-0.06976181268692017,
-0.06762080639600754,
-0.03973694145679474,
-0.029381463304162025,
-0.030384227633476257,
-0.058534715324640274,
0.040545880794525146,
0.030496176332235336,
-0.05247645080089569,
0.041852448135614395,
0.0028102253563702106,
-0.06301800161600113,
-0.0764525830745697,
0.0036070391070097685,
-0.01977556198835373,
-0.00017553710495121777,
0.02720666490495205,
0.03499823808670044,
0.05492860823869705,
-0.05023739114403725,
0.03324321657419205,
-0.020117800682783127,
0.0456300713121891,
0.014805260114371777,
-0.014506982639431953,
0.04362485930323601,
-0.045796267688274384,
0.06859956681728363,
0.030066780745983124,
0.02167087234556675,
-0.02784549444913864,
0.03408932685852051,
-0.001557254116050899,
0.030558723956346512,
0.039187368005514145,
-0.02490176260471344,
0.04279091954231262,
0.010440859943628311,
0.02271318808197975,
0.09996244311332703,
-0.006370532792061567,
0.06415995955467224,
0.04976163059473038,
0.038732416927814484,
-0.033821817487478256,
-0.038674190640449524,
-0.0599697083234787,
0.002513337414711714,
-0.031205007806420326,
-0.018784506246447563,
0.04915561154484749,
-0.028697170317173004,
0.0018201358616352081,
-0.004371538758277893,
0.0030878966208547354,
0.032696306705474854,
-0.026509897783398628,
-0.02737279236316681,
0.010336216539144516,
0.008151290938258171,
-0.0693352147936821,
-0.029972035437822342,
0.03415338322520256,
0.05437477305531502,
-0.2933128774166107,
0.01735704205930233,
0.03898877277970314,
0.000034363005397608504,
-0.037041399627923965,
-0.010301178321242332,
0.07642806321382523,
-0.03588215261697769,
-0.07717720419168472,
-0.019742878153920174,
0.020282037556171417,
0.004756802227348089,
0.02894340083003044,
0.025036118924617767,
-0.035816945135593414,
0.03241584822535515,
0.03456388786435127,
-0.13227316737174988,
0.008897029794752598,
-0.030006738379597664,
0.00960384588688612,
-0.025250034406781197,
0.15413719415664673,
0.006891733501106501,
-0.03124578483402729,
0.017822006717324257,
0.03250471502542496,
0.028484007343649864,
-0.03426728397607803,
-0.011185787618160248,
-0.0038676525000482798,
-0.005980866961181164,
0.029327236115932465,
-0.06556981801986694,
0.01088006142526865,
0.07347778230905533,
-0.00611087353900075,
-0.016501326113939285,
0.016667421907186508,
0.002647912362590432,
-0.004586169496178627,
-0.026097750291228294,
-0.03386419266462326,
-0.06995315104722977,
0.0702776089310646,
-0.053979821503162384,
-0.039278045296669006,
0.008913526311516762,
-0.01566828414797783,
-0.02273484133183956,
-0.00808512419462204,
-0.08724725991487503,
0.002311428776010871,
0.020236646756529808,
0.007241342216730118,
-0.026284752413630486,
-0.04747260734438896,
0.010616432875394821,
-0.05898740887641907,
-0.001628259546123445,
-0.004957687109708786,
0.022240059450268745,
-0.025885263457894325,
0.05300053954124451,
0.04928024113178253
] |
PMID:27241 | Properties of partially purified bovine brain dopamine beta-hydroxylase. | Dopamine beta-hydroxylase has been partially purified from bovine brain. A 140-fold purification factor was achieved using solubilization with Triton X-100, ammonium sulphate fractionation between 20-50 per cent saturation, affinity chromatography on concanavalin A-Sepharose 4 B and then filtration through Sephadex G200. The specific activity at the end was 51 nmoles/h/mg protein. The majority of endogenous inhibitors were lost. Immunological studies, kinetic studies, studies on the interaction with lectins and the effect of carboxylic acids on enzyme activity were carried out. Our data are in favour of the close similarity between the bovine brain and adrenal enzymes. No major differences could be found, at least with the characterization experiments using in the present study. | Properties of partially purified bovine brain dopamine beta-hydroxylase. Dopamine beta-hydroxylase has been partially purified from bovine brain. A 140-fold purification factor was achieved using solubilization with Triton X-100, ammonium sulphate fractionation between 20-50 per cent saturation, affinity chromatography on concanavalin A-Sepharose 4 B and then filtration through Sephadex G200. The specific activity at the end was 51 nmoles/h/mg protein. The majority of endogenous inhibitors were lost. Immunological studies, kinetic studies, studies on the interaction with lectins and the effect of carboxylic acids on enzyme activity were carried out. Our data are in favour of the close similarity between the bovine brain and adrenal enzymes. No major differences could be found, at least with the characterization experiments using in the present study. | [
-0.04633104428648949,
0.0005237354780547321,
-0.064874067902565,
0.020206183195114136,
-0.02293968014419079,
-0.028483130037784576,
0.06968236714601517,
0.03483453765511513,
-0.014459656551480293,
-0.017850792035460472,
-0.01869313232600689,
-0.09344933927059174,
-0.03179921954870224,
0.0534500889480114,
-0.026741238310933113,
-0.027648290619254112,
0.025957534089684486,
-0.008370842784643173,
-0.05356482416391373,
0.011369778774678707,
0.003619399154558778,
-0.00817776657640934,
-0.0036581335589289665,
0.014845099300146103,
0.032402973622083664,
0.01545577310025692,
-0.03750503435730934,
0.05033208429813385,
-0.06547187268733978,
-0.1870346963405609,
0.02941475622355938,
-0.015704123303294182,
0.03773298114538193,
-0.10095556825399399,
0.02498428337275982,
0.04762190580368042,
0.02040221355855465,
-0.020353013649582863,
0.012554415501654148,
0.06537308543920517,
0.0733022466301918,
-0.0260826013982296,
-0.029872629791498184,
0.028287170454859734,
0.024692045524716377,
0.036023035645484924,
-0.08307605981826782,
-0.002219549613073468,
0.0880928486585617,
0.024615786969661713,
0.006735007278621197,
-0.01318354718387127,
-0.025919485837221146,
0.018533548340201378,
0.019435236230492592,
0.009103787131607533,
0.0010014871368184686,
0.07554589211940765,
-0.02487700618803501,
0.07255887240171432,
0.006363929249346256,
0.05579204484820366,
-0.12429159134626389,
0.03802689537405968,
0.0487050823867321,
0.010358333587646484,
-0.048882294446229935,
-0.06538312882184982,
0.06175689399242401,
0.07859475165605545,
-0.0551370270550251,
0.049909114837646484,
0.04783472791314125,
-0.029264073818922043,
-0.018477236852049828,
-0.022555042058229446,
-0.020607320591807365,
0.00020584215235430747,
0.02172049507498741,
0.03415974602103233,
0.03294152393937111,
0.04036247730255127,
0.011609063483774662,
-0.009117229841649532,
-0.009915297850966454,
0.013046784326434135,
0.019298812374472618,
0.005276485346257687,
-0.023583387956023216,
0.0018699232023209333,
-0.04015493765473366,
-0.0225883387029171,
-0.008337094448506832,
0.01051646564155817,
-0.040045350790023804,
0.054720062762498856,
0.014446048066020012,
-0.007669524755328894,
-0.01024002768099308,
0.3740125596523285,
-0.029096387326717377,
0.04579702764749527,
-0.04196571186184883,
-0.0554325133562088,
-0.03712502866983414,
0.06854315102100372,
-0.030907733365893364,
0.0010901925852522254,
-0.013482685200870037,
0.06506753712892532,
0.020391056314110756,
0.026960503309965134,
0.07351727783679962,
-0.03320953622460365,
0.03297010436654091,
-0.04469635710120201,
-0.017966678366065025,
0.00019936943135689944,
0.0023061258252710104,
0.023283841088414192,
-0.04936065524816513,
0.0212157741189003,
0.054815638810396194,
-0.02155962586402893,
-0.004905033390969038,
-0.015342873521149158,
0.010184369049966335,
0.10390771925449371,
0.062023136764764786,
0.002654156181961298,
0.00719206128269434,
0.028999721631407738,
0.002131957560777664,
0.014866024255752563,
0.03846307843923569,
-0.007879715412855148,
0.038300808519124985,
0.027304919436573982,
0.0018535279668867588,
0.011284508742392063,
0.019507762044668198,
0.009414399974048138,
-0.005747626535594463,
-0.075977623462677,
-0.021665386855602264,
0.04161679744720459,
0.07159747928380966,
-0.04400254413485527,
0.0018590303370729089,
0.012323101982474327,
-0.029986895620822906,
0.08690652251243591,
-0.035525307059288025,
0.07620177417993546,
0.004131543915718794,
-0.004505648743361235,
-0.01977529190480709,
-0.03964933007955551,
0.012884609401226044,
-0.015026971697807312,
-0.08517862856388092,
0.00228521297685802,
0.029639191925525665,
0.07204128056764603,
0.036173418164253235,
-0.013080348260700703,
-0.08681388944387436,
0.01154614333063364,
0.053637437522411346,
-0.03673367202281952,
0.023231765255331993,
-0.01037332322448492,
-0.054892588406801224,
-0.021211111918091774,
-0.07861561328172684,
-0.04051506519317627,
0.0006133922724984586,
0.07093190401792526,
0.017551399767398834,
-0.014387254603207111,
0.005157428327947855,
-0.06136852502822876,
-0.06356558203697205,
0.03428352251648903,
0.0014460424426943064,
-0.050809044390916824,
0.004235262982547283,
0.00037221392267383635,
0.0017311512492597103,
0.019229505211114883,
-0.05413292720913887,
0.014430519193410873,
-0.0686410441994667,
-0.02116675302386284,
-0.013384663499891758,
-0.038356713950634,
-0.017442679032683372,
-0.0066231912933290005,
0.005710321478545666,
0.018801143392920494,
0.05007103085517883,
-0.0035555455833673477,
-0.060372836887836456,
0.0741061270236969,
0.01870834082365036,
0.04587491601705551,
0.0022092624567449093,
0.014893940649926662,
0.04662497714161873,
0.036016110330820084,
-0.03532368317246437,
0.003773078788071871,
-0.08651064336299896,
-0.012826023623347282,
-0.06668516248464584,
0.0903393104672432,
0.024051032960414886,
-0.021793929859995842,
0.08952155709266663,
0.01074427179992199,
0.025723794475197792,
-0.05889735743403435,
-0.02321961335837841,
-0.288331001996994,
-0.04747183620929718,
-0.041243817657232285,
-0.014757716096937656,
0.04285668581724167,
-0.09955943375825882,
-0.019561221823096275,
-0.024054544046521187,
0.04562769830226898,
-0.016896085813641548,
0.024377457797527313,
0.03905963897705078,
0.0006643298547714949,
-0.016753576695919037,
-0.008113693445920944,
0.04130869358778,
0.06419629603624344,
-0.060424383729696274,
0.020190341398119926,
0.0034005341585725546,
-0.001394784776493907,
-0.017891738563776016,
0.06443250924348831,
0.006965514272451401,
0.009375340305268764,
-0.04017440974712372,
0.12421315163373947,
0.001998115563765168,
0.036395106464624405,
0.022253641858696938,
-0.03425464406609535,
0.08427421748638153,
-0.054136794060468674,
-0.08925674855709076,
-0.01338629238307476,
-0.09136524796485901,
-0.030050816014409065,
-0.03162684664130211,
0.014750633388757706,
0.010499933734536171,
-0.038384463638067245,
0.02972836047410965,
0.0319523848593235,
-0.05840417370200157,
-0.02359524928033352,
-0.012138274498283863,
0.020579436793923378,
-0.011415188200771809,
-0.05493832752108574,
0.022624481469392776,
0.08491884917020798,
-0.037185173481702805,
0.037684835493564606,
-0.014029155485332012,
-0.006143026519566774,
0.03747258707880974,
-0.08028554916381836,
-0.0012099840678274632,
-0.06556980311870575,
0.009047668427228928,
-0.011674802750349045,
-0.032616011798381805,
-0.047879017889499664,
0.01917835883796215,
-0.014549216255545616,
0.041031453758478165,
0.0018243156373500824,
-0.02975369431078434,
0.024243928492069244,
0.05397828668355942,
0.011855396442115307,
0.06737639755010605,
-0.025227395817637444,
0.04628421366214752,
0.017686938866972923,
0.024227209389209747,
-0.01554357260465622,
-0.03366498276591301,
-0.025547657161951065,
0.05976317822933197,
-0.03140883520245552,
-0.04058085009455681,
0.04577748104929924,
0.013756436295807362,
0.02461247704923153,
0.0031611768063157797,
0.086305633187294,
-0.06158466264605522,
0.02560870163142681,
0.014537785202264786,
-0.018070150166749954,
0.008543466217815876,
-0.030014626681804657,
-0.006831273436546326,
0.0369260348379612,
-0.006750269792973995,
-0.26007187366485596,
-0.011906292289495468,
0.05886426568031311,
0.00990977417677641,
-0.011001905426383018,
0.039619799703359604,
0.06020805612206459,
-0.029402805492281914,
-0.027242418378591537,
-0.007914893329143524,
0.025124138221144676,
0.05776131525635719,
0.0960022360086441,
-0.006718859076499939,
-0.01743795908987522,
0.012577390298247337,
0.0480700358748436,
-0.0024839998222887516,
-0.0007163427653722465,
-0.014188997447490692,
0.0073104905895888805,
0.005249503534287214,
0.16162559390068054,
0.0035990984179079533,
-0.02362743765115738,
0.036609750241041183,
0.009150827303528786,
0.008153872564435005,
-0.0337003730237484,
0.0015227817930281162,
0.0022970475256443024,
-0.02498682029545307,
0.06388944387435913,
-0.04891076311469078,
0.0030064480379223824,
0.03570803254842758,
-0.05264531448483467,
-0.0007872733403928578,
0.03067849390208721,
-0.016315465793013573,
-0.01276326086372137,
-0.017201928421854973,
-0.14977437257766724,
-0.029869912192225456,
0.019447246566414833,
-0.11060626059770584,
-0.06991367787122726,
-0.031686972826719284,
-0.01746438257396221,
-0.02540237456560135,
-0.0293645728379488,
-0.046938277781009674,
-0.010669104754924774,
0.014060547575354576,
-0.03013407438993454,
0.036004096269607544,
0.012866050936281681,
-0.021153012290596962,
-0.031057076528668404,
0.010833264328539371,
-0.0194283127784729,
0.04267935827374458,
0.008449248969554901,
0.11384186148643494,
0.03900117799639702
] |
PMID:27238 | [Effect of pH on the permeability and mechanical properties of intercellular contacts in the small intestinal epithelium]. | It is shown that acid site population controlling ion permeation through cell contacts is localized in the region of tight junction. The isoelectric point of cell surface in the region to tight junction changed from mean pH = 2.37 +/- 0.1 for duodenum to pH =2.80 +/- +/- 0.1 for ileum. The maximum of cell adhesion in the tissue reached at mean pH that corresponding the isoelectric point. It seems possible, that this acid site population take part in interaction of plasma membranes of neighbouring cells. | [Effect of pH on the permeability and mechanical properties of intercellular contacts in the small intestinal epithelium]. It is shown that acid site population controlling ion permeation through cell contacts is localized in the region of tight junction. The isoelectric point of cell surface in the region to tight junction changed from mean pH = 2.37 +/- 0.1 for duodenum to pH =2.80 +/- +/- 0.1 for ileum. The maximum of cell adhesion in the tissue reached at mean pH that corresponding the isoelectric point. It seems possible, that this acid site population take part in interaction of plasma membranes of neighbouring cells. | [
-0.039801884442567825,
-0.037829089909791946,
0.024342430755496025,
0.07246790081262589,
-0.0064039151184260845,
-0.030071428045630455,
0.09120265394449234,
0.060953620821237564,
-0.004484865348786116,
-0.043909281492233276,
0.06732295453548431,
-0.11493515223264694,
0.02982654608786106,
0.0324307419359684,
0.017822755500674248,
-0.011996375396847725,
-0.012448337860405445,
-0.0011416029883548617,
-0.07117325067520142,
-0.010926310904324055,
0.03407924249768257,
-0.032626524567604065,
0.05696658417582512,
-0.04784243181347847,
0.03233223780989647,
-0.013439240865409374,
0.011448310688138008,
0.011615852825343609,
-0.07272514700889587,
-0.17372086644172668,
-0.0032716691493988037,
0.03302214667201042,
0.020292211323976517,
0.0319998599588871,
-0.03071061708033085,
0.003864482045173645,
-0.021694248542189598,
-0.0018849453190341592,
0.020530015230178833,
-0.025010261684656143,
0.029866501688957214,
0.031150629743933678,
0.03200080245733261,
-0.0007984481635503471,
-0.00012653695011977106,
-0.013439410366117954,
-0.003258481854572892,
-0.0401034951210022,
0.059501975774765015,
0.04175514727830887,
0.04377593472599983,
0.03461788594722748,
0.03472293168306351,
0.02401479333639145,
0.00690121203660965,
0.005190015304833651,
0.03461756929755211,
0.02772361785173416,
0.027295248582959175,
-0.001887118094600737,
0.0537261888384819,
0.00869656540453434,
-0.15548720955848694,
0.09203800559043884,
0.06069106608629227,
-0.007890786044299603,
-0.04982786253094673,
0.0016938495682552457,
0.06128297373652458,
0.007943197153508663,
-0.05675339326262474,
0.048146553337574005,
-0.05411878600716591,
-0.020330078899860382,
0.017703372985124588,
-0.052767179906368256,
-0.002321683568879962,
0.019928941503167152,
-0.006053206976503134,
0.022645607590675354,
-0.05159641429781914,
0.018786098808050156,
0.014725308865308762,
-0.009774583391845226,
0.010256984271109104,
-0.015464255586266518,
0.04548706114292145,
-0.03069128654897213,
-0.03499870002269745,
0.006197124719619751,
-0.011209610849618912,
-0.055150263011455536,
-0.08395292609930038,
0.01682705618441105,
-0.026864970102906227,
0.04763343185186386,
0.02145211398601532,
-0.07716018706560135,
-0.029508545994758606,
0.3499678075313568,
-0.07236912101507187,
0.06597952544689178,
-0.054351359605789185,
0.017406754195690155,
0.014690661802887917,
-0.02332570217549801,
-0.03626374527812004,
-0.0010970939183607697,
-0.06574837863445282,
0.08798243850469589,
0.015157653018832207,
0.01903562620282173,
-0.05248192697763443,
-0.03145361691713333,
0.013674967922270298,
0.043234456330537796,
0.07625353336334229,
-0.016454270109534264,
0.04873519763350487,
-0.014894901774823666,
-0.06021849438548088,
0.02433672733604908,
-0.02644105814397335,
-0.017422785982489586,
0.10959548503160477,
0.01741057075560093,
-0.042358800768852234,
0.06954731792211533,
0.03311639651656151,
-0.0379396416246891,
0.026294898241758347,
0.02637321874499321,
0.002583808032795787,
0.00894744973629713,
0.0025338076520711184,
-0.029826289042830467,
-0.01161794550716877,
-0.00707069830968976,
0.049791119992733,
-0.03752078488469124,
-0.03820255026221275,
-0.09988453984260559,
-0.042625900357961655,
-0.09317179769277573,
-0.05247817188501358,
0.10451500117778778,
-0.04945647716522217,
0.01700470596551895,
-0.02232271432876587,
-0.06339191645383835,
0.009352479130029678,
-0.005352537147700787,
-0.025623466819524765,
0.03718636557459831,
0.024718303233385086,
0.030043212696909904,
0.007709227968007326,
-0.00790555588901043,
-0.010821478441357613,
-0.024908553808927536,
-0.03369037061929703,
-0.011743590235710144,
-0.002616805024445057,
0.10788470506668091,
0.042884789407253265,
-0.006933977827429771,
-0.026130467653274536,
-0.04501154646277428,
-0.01682714745402336,
-0.020193908363580704,
-0.031384360045194626,
-0.0016901982016861439,
0.019943803548812866,
-0.030242636799812317,
-0.016402345150709152,
0.05560017749667168,
0.010226939804852009,
-0.0054913717322051525,
0.010464735329151154,
-0.02004891075193882,
0.015437894500792027,
0.04051929712295532,
0.008344503119587898,
0.0033560488373041153,
-0.0061831120401620865,
-0.022154711186885834,
-0.061273083090782166,
-0.004806818440556526,
-0.017084725201129913,
0.06571981310844421,
-0.014019466936588287,
0.03322797268629074,
-0.06965091824531555,
0.0238744355738163,
-0.004222926218062639,
-0.04598226025700569,
-0.011371813714504242,
-0.04764045402407646,
-0.06925027817487717,
0.012112549506127834,
0.03905852511525154,
-0.007145219016820192,
-0.06144294515252113,
0.008132508024573326,
-0.00028284508152864873,
0.03308986499905586,
0.040434714406728745,
0.021228408440947533,
0.018125947564840317,
0.01708075776696205,
-0.018139922991394997,
0.010430035181343555,
-0.039486031979322433,
-0.03508025407791138,
-0.017290571704506874,
-0.012265214696526527,
0.04627026244997978,
0.004855778068304062,
0.10197098553180695,
0.019786426797509193,
0.08740870654582977,
-0.005632208194583654,
-0.02329786866903305,
-0.26630163192749023,
-0.003058249596506357,
-0.04117002710700035,
0.038626834750175476,
0.06671435385942459,
-0.019474349915981293,
0.05199776589870453,
-0.030590901151299477,
0.023827102035284042,
-0.08569464832544327,
0.07916159927845001,
0.05379977822303772,
-0.04768690466880798,
0.022576874122023582,
-0.006847368087619543,
0.02643025666475296,
0.021923959255218506,
0.001858462579548359,
0.05698910355567932,
0.0013227710733190179,
0.07571233808994293,
-0.031922850757837296,
-0.006386148743331432,
0.05953199043869972,
0.04454277828335762,
0.00864876713603735,
0.1297122687101364,
-0.007747449912130833,
-0.023706821724772453,
-0.08131664246320724,
0.017069386318325996,
0.004366485867649317,
0.016646798700094223,
-0.04385850206017494,
-0.01624322682619095,
0.00743341539055109,
0.009553423151373863,
-0.03991912305355072,
0.027703670784831047,
-0.04174956679344177,
-0.09507642686367035,
-0.0020077142398804426,
-0.05434146896004677,
-0.02355192042887211,
-0.013956412672996521,
-0.028210023418068886,
0.037033285945653915,
-0.010242502205073833,
-0.03729984164237976,
-0.02049521915614605,
0.05439557880163193,
-0.04810723662376404,
0.002295486396178603,
-0.04682451859116554,
0.03229399397969246,
-0.012091577053070068,
0.05956300348043442,
0.030716771259903908,
-0.09663986414670944,
0.008730856701731682,
-0.05781388282775879,
-0.028114397078752518,
0.06497535854578018,
0.003870725864544511,
-0.021421993151307106,
0.05631733685731888,
0.03508621081709862,
0.0433349609375,
0.02369675599038601,
0.006998964119702578,
0.045381732285022736,
0.10526561737060547,
-0.06596101820468903,
0.008979512378573418,
0.021024415269494057,
-0.020938914269208908,
-0.014780228957533836,
-0.00930624920874834,
-0.040479876101017,
-0.06219012662768364,
0.04418576881289482,
-0.04963088035583496,
0.05740371346473694,
-0.022238126024603844,
0.005135813262313604,
0.08524972945451736,
0.047806255519390106,
-0.06409909576177597,
-0.032691918313503265,
-0.009582284837961197,
0.016854098066687584,
0.02575703337788582,
-0.06657508760690689,
-0.0018772950861603022,
0.023429250344634056,
-0.02517235465347767,
-0.30067187547683716,
0.07840196788311005,
0.0020276238210499287,
-0.0097010163590312,
-0.06769142299890518,
0.010143100284039974,
0.032553330063819885,
-0.008225178346037865,
-0.0167932640761137,
0.0652787834405899,
0.014902433380484581,
-0.010564545169472694,
0.05057687312364578,
0.04527290165424347,
-0.00014739383186679333,
-0.033573273569345474,
0.017595332115888596,
-0.060267094522714615,
0.011888734996318817,
-0.02032262273132801,
0.021868189796805382,
-0.06511298567056656,
0.17911973595619202,
-0.015811122953891754,
0.004468776285648346,
0.017089372500777245,
-0.019930418580770493,
0.043648023158311844,
0.03471280261874199,
-0.02239117957651615,
0.006982034537941217,
0.053919717669487,
-0.027664240449666977,
0.005119102541357279,
0.04625869169831276,
0.0007962069939821959,
-0.0029695213306695223,
-0.017555901780724525,
-0.0303326565772295,
0.003255389630794525,
0.0054410407319664955,
-0.008203301578760147,
-0.05001712217926979,
-0.07360543310642242,
0.020965613424777985,
-0.014148433692753315,
-0.013337960466742516,
-0.03659864515066147,
0.026942573487758636,
-0.013414546847343445,
-0.047642193734645844,
-0.05544934421777725,
0.07543554902076721,
0.000599664868786931,
0.05150558799505234,
-0.010805336758494377,
-0.01365894265472889,
-0.010562019422650337,
0.004291991703212261,
-0.005394608713686466,
0.10596542805433273,
0.017139306291937828,
0.0009254573378711939,
0.025065287947654724,
0.04939013347029686
] |
PMID:27242 | New trends in cryoenzymology: I.-Supercooled aqueous solutions. | A water in oil emulsion technique is proposed to investigate enzyme catalyzed reactions at sub-zero temperatures in the supercooled liquid state to avoid some reversible effects of the usual cosolvents on kinetics. Some results are listed: potentialities and technical problems of the procedure are discussed. | New trends in cryoenzymology: I.-Supercooled aqueous solutions. A water in oil emulsion technique is proposed to investigate enzyme catalyzed reactions at sub-zero temperatures in the supercooled liquid state to avoid some reversible effects of the usual cosolvents on kinetics. Some results are listed: potentialities and technical problems of the procedure are discussed. | [
-0.06010263040661812,
0.02047523483633995,
-0.010548132471740246,
0.015904327854514122,
0.0003723761474248022,
-0.04218008741736412,
-0.0004591558245010674,
0.01814911887049675,
-0.02775525487959385,
-0.024700336158275604,
0.020914211869239807,
-0.009081104770302773,
-0.011113121174275875,
-0.014764352701604366,
-0.010390612296760082,
0.0016803082544356585,
0.04224582761526108,
0.03479687497019768,
-0.08011700212955475,
0.06431427597999573,
0.018269086256623268,
0.006124510429799557,
0.026672808453440666,
-0.03367837890982628,
-0.007722285110503435,
0.06571048498153687,
0.03846263512969017,
0.05335942655801773,
-0.05477370694279671,
-0.18622855842113495,
0.016259241849184036,
-0.0637725442647934,
0.049671486020088196,
0.012083732523024082,
0.06263528764247894,
-0.0036969915963709354,
-0.009173739701509476,
-0.0021815320942550898,
-0.0691368505358696,
0.020992528647184372,
0.024335971102118492,
0.06888586282730103,
-0.03960404917597771,
-0.011073371395468712,
0.0046170782297849655,
0.0312880277633667,
-0.02563430555164814,
0.024930214509367943,
0.024941040202975273,
-0.028541965410113335,
0.02321123518049717,
0.03495730087161064,
-0.05608667433261871,
-0.009729345329105854,
0.03467842936515808,
-0.010251732543110847,
-0.005802822764962912,
-0.025753062218427658,
0.00882614217698574,
0.009229028597474098,
-0.014597129076719284,
0.025857476517558098,
-0.11486759036779404,
0.06666804105043411,
0.10711194574832916,
-0.01766986772418022,
0.0001324015756836161,
-0.003617258043959737,
0.024480417370796204,
0.0053760455921292305,
-0.04794951155781746,
0.05515722930431366,
0.026151444762945175,
-0.014752638526260853,
-0.01810595393180847,
-0.06360575556755066,
0.0012401728890836239,
0.020203111693263054,
-0.01000914815813303,
-0.009333133697509766,
0.008633832447230816,
-0.06645337492227554,
0.04226398095488548,
-0.004553177393972874,
-0.06256286799907684,
-0.005592727102339268,
-0.03849609196186066,
-0.01151797641068697,
0.06669186055660248,
0.05654863640666008,
-0.014872818253934383,
-0.04037933796644211,
0.00617552362382412,
0.024666903540492058,
-0.04468723386526108,
0.09508189558982849,
0.02172483131289482,
0.02370096929371357,
0.060312557965517044,
0.4264010488986969,
-0.01645742729306221,
0.020812099799513817,
-0.03351498767733574,
-0.04290551319718361,
-0.0646325945854187,
-0.032175496220588684,
-0.059710387140512466,
-0.028664713725447655,
0.011685856617987156,
0.014775700867176056,
0.05277419835329056,
-0.03402185067534447,
0.03834652528166771,
-0.1033862978219986,
0.0020215166732668877,
0.023719478398561478,
0.009919311851263046,
-0.008232216350734234,
-0.048464421182870865,
0.014245647005736828,
-0.038088034838438034,
0.023230958729982376,
0.011877335608005524,
0.0032908387947827578,
0.05912086367607117,
-0.004583617206662893,
0.0015235829632729292,
0.0763590931892395,
-0.018330618739128113,
-0.018506735563278198,
0.014231228269636631,
-0.00483601214364171,
-0.041272323578596115,
-0.012891898863017559,
-0.03568601980805397,
0.028486212715506554,
0.03648031875491142,
-0.015538740903139114,
0.06202949583530426,
0.028079969808459282,
0.0229558814316988,
0.005913973320275545,
-0.017949815839529037,
-0.09359651058912277,
0.00904703140258789,
0.06171397119760513,
-0.010154289193451405,
0.06700403988361359,
-0.04848797991871834,
0.016961565241217613,
0.005839166231453419,
0.03453148156404495,
-0.06169795244932175,
0.04402230307459831,
-0.00888916663825512,
-0.03218354657292366,
0.03506201505661011,
-0.028692789375782013,
-0.03561914339661598,
0.028638578951358795,
-0.09751443564891815,
-0.006239443551748991,
-0.01155165582895279,
0.11687198281288147,
0.006446569226682186,
-0.042032577097415924,
0.025336863473057747,
-0.040061041712760925,
0.029491756111383438,
-0.034246597439050674,
-0.030216660350561142,
-0.00903206318616867,
0.020679516717791557,
0.01240235473960638,
-0.0044040498323738575,
0.06799986213445663,
0.04835347458720207,
-0.005450016353279352,
-0.012570846825838089,
0.027522996068000793,
0.02095990628004074,
-0.005290582310408354,
0.01756817102432251,
0.033235594630241394,
0.04076550155878067,
-0.03130873665213585,
-0.03630570322275162,
0.01886552758514881,
-0.06193093582987785,
0.05833866074681282,
-0.061066217720508575,
-0.008122734725475311,
-0.07603052258491516,
0.02984457276761532,
-0.013918820768594742,
-0.013461228460073471,
0.004664343781769276,
-0.009458144195377827,
0.0475291982293129,
-0.01808980666100979,
0.13651953637599945,
0.011489955708384514,
-0.023584576323628426,
0.03012584149837494,
0.05414370819926262,
0.0011157351545989513,
0.057457830756902695,
0.029516832903027534,
0.03788048028945923,
0.049289267510175705,
-0.040913280099630356,
-0.04516306519508362,
-0.043039776384830475,
-0.006111322436481714,
-0.051537904888391495,
0.01977546326816082,
0.008002052083611488,
-0.017051588743925095,
-0.011964380741119385,
-0.028251031413674355,
0.05758463963866234,
-0.05650080740451813,
-0.06576678901910782,
-0.3002024292945862,
-0.03931257128715515,
-0.03508841618895531,
-0.06167139485478401,
0.07380352169275284,
-0.06649903953075409,
0.020734522491693497,
-0.06472242623567581,
0.011142155155539513,
0.001828035106882453,
0.016677211970090866,
0.025462130084633827,
0.011518033221364021,
-0.02332068234682083,
0.013580858707427979,
-0.021427782252430916,
0.021326962858438492,
-0.0712122768163681,
0.022380216047167778,
0.012936742976307869,
-0.04067400470376015,
0.010553507134318352,
-0.02028425969183445,
0.014234557747840881,
0.06998278200626373,
-0.05751625820994377,
0.12166942656040192,
0.03977461904287338,
0.007948160171508789,
-0.005355559755116701,
-0.01297947857528925,
0.006651701871305704,
-0.0003019248542841524,
-0.11042764782905579,
-0.07316221296787262,
-0.04166354984045029,
0.0011908670421689749,
-0.04963996633887291,
0.021151751279830933,
-0.007153114769607782,
-0.0016849723178893328,
0.0489887110888958,
0.05927800387144089,
0.01010757964104414,
-0.03289872035384178,
-0.00018361839465796947,
0.014829111285507679,
0.01527431420981884,
-0.057964250445365906,
0.020460793748497963,
0.08428928256034851,
-0.009327614679932594,
0.03716498613357544,
-0.0105829406529665,
-0.045519307255744934,
0.016075368970632553,
0.018908798694610596,
-0.006965650245547295,
0.005728799384087324,
-0.024384867399930954,
0.04385499283671379,
0.018964366987347603,
-0.00981647614389658,
0.08416606485843658,
-0.028032749891281128,
0.008722543716430664,
0.010483279824256897,
-0.023392407223582268,
0.017026184126734734,
-0.05072828382253647,
0.0019604875706136227,
0.09204266965389252,
-0.060338981449604034,
0.014935119077563286,
0.029251940548419952,
0.07527076452970505,
0.025508852675557137,
-0.06055770441889763,
-0.03986842557787895,
0.012114031240344048,
0.02071079984307289,
-0.030649635940790176,
0.03280190750956535,
0.01358963456004858,
-0.006960668135434389,
0.04386918246746063,
-0.015883609652519226,
-0.06852730363607407,
-0.008281504735350609,
-0.0017863604007288814,
0.02000119909644127,
-0.01977996714413166,
-0.04794710874557495,
0.028191599994897842,
-0.007887763902544975,
0.03324166685342789,
-0.24869276583194733,
0.021534929051995277,
0.03454532101750374,
-0.01001623086631298,
0.0025223323609679937,
-0.014971564523875713,
0.01841200329363346,
0.011277534998953342,
-0.03707671910524368,
0.05993882939219475,
-0.03304038196802139,
0.021864548325538635,
0.08626119792461395,
0.07234221696853638,
-0.014133745804429054,
-0.0024661708157509565,
0.009416847489774227,
-0.0452604666352272,
0.012204966507852077,
0.009221005253493786,
0.010772822424769402,
0.010904052294790745,
0.19147764146327972,
0.0018783601699396968,
-0.02773882821202278,
0.01699337735772133,
-0.0012974797282367945,
0.048568449914455414,
-0.048025425523519516,
-0.008458114229142666,
0.006829860154539347,
0.01851791888475418,
-0.035949092358350754,
-0.012922571040689945,
0.005364805925637484,
0.043056223541498184,
-0.014147050678730011,
0.01421100553125143,
-0.008756563067436218,
0.03790269419550896,
0.02889622561633587,
-0.03215455263853073,
-0.018597515299916267,
-0.08190181851387024,
0.004767365287989378,
-0.07226435095071793,
-0.0051343259401619434,
-0.04173164814710617,
-0.05576734244823456,
-0.013312284834682941,
-0.01833830028772354,
-0.04587505757808685,
0.0559905506670475,
0.017204923555254936,
-0.005571329966187477,
0.054938867688179016,
-0.032687995582818985,
-0.04631924256682396,
-0.05689239129424095,
0.003405475290492177,
0.023739555850625038,
0.0023228321224451065,
-0.03958511725068092,
0.09431061148643494,
0.023798439651727676
] |
PMID:27239 | [Phosphorylation in acid shocked chloroplasts]. | ADP phosphorylation up to 2.5 nmol per mg of chlorphyll has been found in chloroplasts from Vicia faba plants when the suspension was sharply acidified from pH 8 to pH 1.6 with excess of HC1/KC1 solution. This fact appears to be due to confromation relaxation of the coupling factor under acid-induced denaturation. Heat pretreatment inhibits this effect. | [Phosphorylation in acid shocked chloroplasts]. ADP phosphorylation up to 2.5 nmol per mg of chlorphyll has been found in chloroplasts from Vicia faba plants when the suspension was sharply acidified from pH 8 to pH 1.6 with excess of HC1/KC1 solution. This fact appears to be due to confromation relaxation of the coupling factor under acid-induced denaturation. Heat pretreatment inhibits this effect. | [
-0.07117976993322372,
-0.059142064303159714,
0.012414447031915188,
0.01675734668970108,
0.05194719508290291,
-0.038917042315006256,
0.05515793338418007,
-0.01531151495873928,
-0.03228100761771202,
-0.028416475281119347,
0.07354756444692612,
-0.1138739362359047,
0.002663230523467064,
0.0368911549448967,
-0.017806006595492363,
-0.005419040098786354,
-0.02369699999690056,
0.0020431834273040295,
-0.06420154124498367,
-0.014809297397732735,
0.07316020876169205,
0.01324306707829237,
-0.016810817644000053,
-0.02848823554813862,
0.03742830827832222,
0.047374874353408813,
-0.023020241409540176,
0.010128870606422424,
-0.03764742240309715,
-0.18292714655399323,
0.006987473461776972,
0.024702604860067368,
0.016872772946953773,
-0.07806363701820374,
-0.012810797430574894,
-0.005709643475711346,
-0.010879366658627987,
-0.0045056031085550785,
-0.03358472138643265,
0.051234837621450424,
0.027795275673270226,
0.024753795936703682,
-0.04215163737535477,
-0.0007343706674873829,
-0.0012608637334778905,
-0.022917622700333595,
-0.017967859283089638,
-0.0007918057963252068,
0.07388796657323837,
0.012648262083530426,
-0.0067923907190561295,
0.023066909983754158,
0.0410638153553009,
0.011074437759816647,
-0.02596326544880867,
0.020212354138493538,
0.04259869083762169,
0.042809706181287766,
0.06732174009084702,
-0.04074558988213539,
0.006824456620961428,
0.07218031585216522,
-0.14648117125034332,
0.0009345314465463161,
0.04819803684949875,
-0.016455698758363724,
-0.0028268839232623577,
-0.017148366197943687,
0.036501649767160416,
0.02312820591032505,
-0.08934434503316879,
0.005408523138612509,
-0.00685868551954627,
-0.017434827983379364,
0.036866702139377594,
-0.024564072489738464,
0.004669437650591135,
-0.06458248943090439,
0.006331437733024359,
-0.03275484964251518,
-0.019795985892415047,
0.003997058141976595,
-0.029320530593395233,
0.0186009481549263,
-0.014605800621211529,
0.012467717751860619,
0.05415147915482521,
0.020234927535057068,
-0.00805511511862278,
-0.0035346539225429296,
0.001512714079581201,
-0.10194572061300278,
0.0033073488157242537,
0.017099522054195404,
-0.06958752125501633,
0.06668365746736526,
0.0090629318729043,
-0.012638351880013943,
-0.005474455654621124,
0.3808373808860779,
-0.08235414326190948,
0.09599904716014862,
-0.026726707816123962,
-0.006873087491840124,
-0.008237265981733799,
-0.034610964357852936,
-0.022407133132219315,
0.009347558952867985,
-0.018627461045980453,
0.06343646347522736,
0.05575534701347351,
0.004381864797323942,
0.006867366377264261,
-0.03738025203347206,
0.03207797184586525,
-0.024500517174601555,
-0.01840236410498619,
-0.03461102396249771,
-0.0494254007935524,
0.05853342264890671,
0.01686713844537735,
0.011988092213869095,
0.061473652720451355,
0.016178175806999207,
0.06769132614135742,
0.01831972226500511,
0.0017568914918228984,
0.09080380201339722,
0.0799509808421135,
0.007679459173232317,
0.03331606090068817,
0.019782915711402893,
0.02606092020869255,
-0.012297067791223526,
0.00021541633759625256,
-0.01939685270190239,
0.0797065794467926,
-0.07408838719129562,
0.0351264588534832,
0.021902862936258316,
-0.03091283328831196,
-0.06739763915538788,
-0.038150183856487274,
-0.048009105026721954,
-0.029694275930523872,
0.10235361754894257,
0.0074567231349647045,
0.04176401346921921,
-0.03948582336306572,
0.04944310709834099,
-0.002708808286115527,
0.026872139424085617,
-0.00848530512303114,
0.013440266251564026,
0.013869421556591988,
0.04559537023305893,
0.0002581173612270504,
0.028133118525147438,
0.006276705767959356,
-0.055893681943416595,
-0.06498592346906662,
-0.0037783312145620584,
0.050690505653619766,
0.06099342554807663,
0.019156169146299362,
-0.004344912711530924,
0.0448286198079586,
-0.02877913787961006,
-0.031469136476516724,
-0.00023106484150048345,
0.010160544887185097,
0.05795159190893173,
0.02308809384703636,
-0.0552884042263031,
-0.006590668577700853,
0.005846706219017506,
0.005360226146876812,
-0.014350962825119495,
0.007815984077751637,
-0.03774826228618622,
0.007257396355271339,
0.009942671284079552,
0.06209338828921318,
0.013043309561908245,
0.08768986165523529,
-0.04093029350042343,
-0.017788803204894066,
0.05062178149819374,
-0.04731239005923271,
0.06005021557211876,
-0.055763643234968185,
0.006507467944175005,
-0.04753515124320984,
0.026438482105731964,
-0.0833493173122406,
-0.06358988583087921,
-0.010539217852056026,
0.0294165201485157,
0.01409878395497799,
0.007561939302831888,
0.04150261729955673,
-0.004426105413585901,
-0.0695071667432785,
0.038386810570955276,
-0.013254757039248943,
0.0017573368968442082,
-0.022963648661971092,
0.037731148302555084,
0.024058399721980095,
0.039956387132406235,
-0.03117896057665348,
-0.03684002161026001,
-0.0367022268474102,
0.01494419202208519,
-0.08537046611309052,
0.01826031133532524,
-0.04908673092722893,
0.052661482244729996,
0.029184803366661072,
-0.047811392694711685,
0.0026355606969445944,
-0.0946347564458847,
-0.09384427219629288,
-0.2640872895717621,
-0.05440424755215645,
0.055974721908569336,
0.001953814411535859,
0.06254599988460541,
-0.005436046980321407,
0.0264411773532629,
-0.024812327697873116,
0.03492014855146408,
-0.08029498904943466,
0.012664159759879112,
0.008339906111359596,
-0.015718575567007065,
-0.013303191401064396,
0.027396082878112793,
0.04728945344686508,
0.017442798241972923,
-0.029403366148471832,
0.029226282611489296,
0.007107291836291552,
0.0558793842792511,
0.011201482266187668,
-0.06052130088210106,
0.03267437964677811,
0.021832473576068878,
0.013966245576739311,
0.05675310641527176,
0.0041224341839551926,
-0.03145107254385948,
-0.016710231080651283,
0.02448602393269539,
-0.01757923886179924,
0.015099111944437027,
-0.0336083322763443,
-0.10555016249418259,
0.030362527817487717,
0.015826057642698288,
-0.041011661291122437,
0.05547187477350235,
0.026250576600432396,
0.001239518984220922,
0.005602630786597729,
-0.1397470384836197,
-0.03942565619945526,
-0.026172973215579987,
0.004087383393198252,
0.0497976616024971,
0.07719741761684418,
0.018021216616034508,
0.006567585282027721,
0.03849168121814728,
0.04442112147808075,
0.001298902672715485,
-0.012424479238688946,
0.02104923501610756,
0.01164950616657734,
-0.020617550238966942,
0.06166515871882439,
-0.06571292877197266,
-0.03727522864937782,
-0.00033450667979195714,
-0.04030270501971245,
-0.03177200257778168,
0.08248601108789444,
0.030493376776576042,
-0.024378037080168724,
0.011496168561279774,
0.024118078872561455,
0.026058003306388855,
-0.01659085974097252,
0.016112525016069412,
0.047864798456430435,
-0.01448570005595684,
-0.006628379225730896,
0.053602803498506546,
0.04384978488087654,
-0.0739201158285141,
-0.06851019710302353,
-0.09979429841041565,
-0.02593405358493328,
-0.0005567996995523572,
0.023311752825975418,
0.07566593587398529,
-0.01712043210864067,
-0.07287470996379852,
0.04844103753566742,
0.011920034885406494,
-0.02340155839920044,
-0.033306557685136795,
-0.02063865214586258,
-0.02471737749874592,
-0.023663552477955818,
-0.03353900462388992,
-0.0055580707266926765,
0.05872386693954468,
0.0035961109679192305,
-0.2729361951351166,
-0.00007267432374646887,
0.05357075855135918,
0.02976076304912567,
-0.05260865017771721,
0.08272741734981537,
0.07613407075405121,
-0.0001346758654108271,
-0.0160041693598032,
0.059869591146707535,
-0.02647506445646286,
0.033182088285684586,
0.025368044152855873,
0.062206853181123734,
0.0036111825611442327,
0.03738382086157799,
-0.008325613103806973,
-0.09035634249448776,
0.003991967532783747,
0.015921076759696007,
-0.039133477956056595,
-0.0467909537255764,
0.12259680032730103,
0.014149726368486881,
-0.04377976059913635,
0.06530489772558212,
-0.013552166521549225,
0.045967359095811844,
0.04132761061191559,
-0.019900435581803322,
-0.010475791059434414,
0.06789799779653549,
-0.012583877891302109,
-0.03399353846907616,
0.040777966380119324,
0.022705700248479843,
0.01930297538638115,
-0.016912290826439857,
-0.016580894589424133,
0.03551806882023811,
0.008846971206367016,
-0.015206127427518368,
-0.029550649225711823,
-0.04893655329942703,
0.05936272442340851,
-0.07018003612756729,
-0.036790840327739716,
-0.08814027905464172,
-0.02408568747341633,
-0.012126450426876545,
-0.04332440346479416,
-0.017931118607521057,
0.07801027595996857,
0.04801943898200989,
-0.027626842260360718,
-0.00251812138594687,
-0.030067887157201767,
-0.010718186385929585,
-0.0044634584337472916,
-0.04817497730255127,
0.05030827224254608,
0.022700326517224312,
-0.010442092083394527,
0.028899334371089935,
0.06609714031219482
] |
PMID:27244 | Capacity for biosynthesis of prostaglandin-related compounds: distribution and properties of the rate-limiting enzyme in hydrocorals, gorgoniants, and other coelenterates of the Caribbean and Pacific. | A convenient and reliable assay is described for PGEP synthetase, the rate-limiting enzyme determining the total capacity for biosynthesis of prostaglandin-related compounds. Results of such assays, performed withe fresh specimens under both field and laboratory conditions, newly identify several marine coelenterate species as potentially important resources of PGRCs for research and possible development. Properties of the typical marine coelenterate PGEP synthetase, and the reaction which this enzyme catalyzes, have been further characterized. | Capacity for biosynthesis of prostaglandin-related compounds: distribution and properties of the rate-limiting enzyme in hydrocorals, gorgoniants, and other coelenterates of the Caribbean and Pacific. A convenient and reliable assay is described for PGEP synthetase, the rate-limiting enzyme determining the total capacity for biosynthesis of prostaglandin-related compounds. Results of such assays, performed withe fresh specimens under both field and laboratory conditions, newly identify several marine coelenterate species as potentially important resources of PGRCs for research and possible development. Properties of the typical marine coelenterate PGEP synthetase, and the reaction which this enzyme catalyzes, have been further characterized. | [
-0.04323924705386162,
-0.016294723376631737,
-0.01931893825531006,
-0.02247685194015503,
-0.005781005136668682,
-0.0298606064170599,
0.022826289758086205,
-0.0023992620408535004,
-0.052666593343019485,
-0.0007995431660674512,
0.05240222066640854,
-0.09344718605279922,
-0.02124933898448944,
-0.012214857153594494,
-0.06088031083345413,
-0.01876026578247547,
0.035824451595544815,
0.024560827761888504,
-0.04317942261695862,
0.0018918939167633653,
0.05408250540494919,
-0.00008762778452364728,
0.005992886144667864,
-0.07159674912691116,
0.027859756723046303,
0.0281989648938179,
-0.010114678181707859,
0.017821257933974266,
-0.04707811772823334,
-0.20335087180137634,
0.02604551613330841,
0.014427389949560165,
-0.0018725411500781775,
-0.020920483395457268,
-0.005708235315978527,
0.006122637540102005,
-0.03269719332456589,
-0.06725463271141052,
0.02134493738412857,
0.10935867577791214,
-0.006863887421786785,
0.04415307566523552,
0.009661185555160046,
0.04473540559411049,
0.05079028010368347,
0.0009038809803314507,
-0.028886253014206886,
0.038595180958509445,
0.052353888750076294,
-0.04129962995648384,
0.03876310959458351,
-0.024597231298685074,
-0.0230665635317564,
0.0071466341614723206,
0.022049490362405777,
-0.012380425818264484,
0.010957811959087849,
0.04638944938778877,
-0.02926299162209034,
-0.015438558533787727,
0.029396064579486847,
0.08010681718587875,
-0.1719467043876648,
-0.011451983824372292,
0.01739303022623062,
0.042499370872974396,
-0.02342930994927883,
-0.013091962784528732,
-0.007115327287465334,
0.018735209479928017,
-0.05602097138762474,
0.045444000512361526,
-0.04801647737622261,
-0.011047078296542168,
0.030721835792064667,
0.07975705713033676,
-0.02571815438568592,
0.02326967753469944,
0.024358123540878296,
0.006845218129456043,
0.04896099120378494,
0.01234468724578619,
-0.019150611013174057,
0.0010932068107649684,
-0.014049162156879902,
-0.04987515136599541,
0.018454384058713913,
0.013094351626932621,
0.027270589023828506,
0.05553067848086357,
-0.0166337713599205,
-0.0757194235920906,
0.004375454504042864,
-0.018210142850875854,
-0.07114226371049881,
0.0735391154885292,
0.01622091419994831,
0.02899559773504734,
-0.008317215368151665,
0.4177859425544739,
-0.0638229176402092,
0.03892546147108078,
-0.020374197512865067,
-0.009320133365690708,
0.040269896388053894,
-0.021064890548586845,
0.002309611300006509,
-0.020903196185827255,
0.01017887145280838,
-0.005546923261135817,
0.044362008571624756,
-0.006906344089657068,
0.03264360502362251,
-0.03084641508758068,
0.0017250333912670612,
-0.03493526950478554,
-0.004409210756421089,
-0.010950256139039993,
-0.04408187046647072,
0.04714616760611534,
-0.0365852415561676,
-0.03740769624710083,
0.026911940425634384,
0.05317739024758339,
0.03981850668787956,
-0.012218520045280457,
-0.0183847788721323,
0.06357526779174805,
0.05463392660021782,
-0.006420644000172615,
0.03558209910988808,
0.06164390221238136,
-0.022588716819882393,
0.02650044858455658,
-0.012669632211327553,
-0.041300468146800995,
0.050961583852767944,
-0.004742990247905254,
0.02953318879008293,
-0.00945733767002821,
-0.04406662657856941,
0.008696840144693851,
-0.0019201599061489105,
-0.028012266382575035,
-0.0003106180520262569,
0.10948091000318527,
0.050261881202459335,
0.07346782833337784,
-0.03636031225323677,
0.024248437955975533,
-0.000955852388869971,
0.030796583741903305,
-0.03159737214446068,
-0.005084979813545942,
-0.01294386014342308,
-0.03779573738574982,
-0.008708336390554905,
-0.04506659507751465,
-0.01636246033012867,
-0.011433116160333157,
-0.04812463000416756,
0.04026095196604729,
0.010198784992098808,
0.06455430388450623,
0.04039182513952255,
-0.028679251670837402,
-0.02818671613931656,
0.03081064671278,
-0.015574288554489613,
-0.001315622590482235,
0.017195094376802444,
0.026581158861517906,
0.011563291773200035,
-0.026065055280923843,
-0.020780490711331367,
0.043086566030979156,
0.03816022351384163,
0.03747863322496414,
0.04139553755521774,
0.006991419475525618,
0.03970463573932648,
0.02215551771223545,
-0.03921733424067497,
0.007164903450757265,
-0.02919684909284115,
-0.012201406992971897,
-0.0187249593436718,
-0.012899987399578094,
-0.021519707515835762,
0.023714294657111168,
-0.006397802382707596,
-0.0033454622607678175,
-0.03730021417140961,
0.056803811341524124,
-0.035072218626737595,
-0.0553661547601223,
-0.014892583712935448,
0.025181466713547707,
-0.010710708796977997,
-0.05657330900430679,
0.1049814373254776,
-0.03403107449412346,
0.0038897916674613953,
0.041703447699546814,
-0.012733976356685162,
0.04719715192914009,
0.006134664639830589,
0.05821169912815094,
-0.004525892436504364,
0.04795565828680992,
0.011334477923810482,
-0.053829167038202286,
-0.019497031345963478,
0.024224361404776573,
-0.08944324404001236,
0.05589769408106804,
-0.023033438250422478,
-0.0013056929456070065,
0.03861300274729729,
-0.004560534376651049,
0.0068440972827374935,
-0.04103151708841324,
-0.0007543905521743,
-0.309467077255249,
-0.04986900836229324,
-0.010890783742070198,
-0.035144560039043427,
0.07435774058103561,
0.00984321627765894,
0.002057835925370455,
-0.020466463640332222,
-0.007927028462290764,
-0.028158962726593018,
0.021032555028796196,
0.05514710023999214,
-0.0012886460172012448,
0.06430597603321075,
0.010171976871788502,
0.04628179967403412,
0.03165876865386963,
-0.05015648901462555,
0.028147529810667038,
0.0010158804943785071,
-0.019873730838298798,
-0.053786277770996094,
-0.023956017568707466,
-0.009576570242643356,
0.03856860473752022,
0.006884733214974403,
0.10596683621406555,
0.011595232412219048,
-0.012036263011395931,
-0.020670736208558083,
-0.014881867915391922,
0.04009338468313217,
-0.010131939314305782,
-0.11881770193576813,
0.015579774975776672,
-0.029846535995602608,
-0.0016820355085656047,
-0.05470404028892517,
0.03498952090740204,
-0.03268890827894211,
0.03719612956047058,
0.028420457616448402,
0.00337239820510149,
-0.06622392684221268,
-0.0633627325296402,
-0.0526903010904789,
0.00040685496060177684,
0.046632856130599976,
-0.010397092439234257,
-0.022394800558686256,
0.025146303698420525,
0.02790309675037861,
0.04427395388484001,
-0.045602548867464066,
-0.02320627123117447,
-0.021875599399209023,
-0.042814552783966064,
-0.007665460929274559,
-0.061196643859148026,
-0.012297543697059155,
0.04085617884993553,
-0.011519349180161953,
0.007283573504537344,
0.02560027502477169,
0.005714572034776211,
0.017604628577828407,
-0.004548703320324421,
-0.020777519792318344,
0.028358882293105125,
0.038713857531547546,
0.03930465131998062,
0.07034114003181458,
-0.04961461201310158,
-0.024943005293607712,
-0.003445093287155032,
-0.012132056057453156,
-0.010860406793653965,
-0.04960756003856659,
-0.04930780082941055,
-0.006670585833489895,
0.014475002884864807,
-0.061056990176439285,
0.036767661571502686,
-0.04480750858783722,
0.019635852426290512,
0.010171767324209213,
0.0275573693215847,
-0.03922375664114952,
0.061312343925237656,
0.038188762962818146,
0.005502318497747183,
0.009422115050256252,
-0.010488287545740604,
-0.028079673647880554,
-0.012932942248880863,
0.045643821358680725,
-0.2838701903820038,
-0.015607192181050777,
0.036757100373506546,
-0.0016022395575419068,
-0.021997258067131042,
0.03326200321316719,
0.04118271544575691,
-0.03225104138255119,
-0.020114583894610405,
0.014282810501754284,
0.028956733644008636,
0.035311054438352585,
0.029306121170520782,
0.1007295548915863,
0.0042797173373401165,
0.02661946415901184,
0.021437374874949455,
-0.055375538766384125,
0.07974431663751602,
-0.07685283571481705,
-0.009614075534045696,
-0.05502534285187721,
0.1382225751876831,
0.053529754281044006,
-0.00751465605571866,
0.0377400740981102,
0.04815036803483963,
0.022837365046143532,
-0.07365723699331284,
0.031118672341108322,
-0.02755638025701046,
0.03329329937696457,
0.059328045696020126,
-0.07513053715229034,
-0.013928967528045177,
0.1255311220884323,
-0.012408819980919361,
-0.001686096889898181,
-0.017484309151768684,
-0.031052116304636,
-0.005151345860213041,
-0.02819572575390339,
-0.001458511222153902,
0.02851364016532898,
0.05772382766008377,
-0.12069673836231232,
-0.04713796079158783,
-0.042155276983976364,
-0.027834296226501465,
0.018094226717948914,
-0.06139809638261795,
-0.027386534959077835,
0.029822681099176407,
-0.024994060397148132,
-0.00972158182412386,
0.014108424074947834,
-0.013014725409448147,
-0.04726795479655266,
0.0087277265265584,
-0.03267611190676689,
-0.009001074358820915,
-0.04729717597365379,
-0.01778041385114193,
0.12186423689126968,
0.038181472569704056
] |
PMID:27245 | [Kinetics and mechanism of nucleophilic effect on the oxidation of o-dianisidine catalyzed by horseradish peroxidase]. | The effects of benzimidazole and 4-nitroimidazole on the reaction of o-dianisidine peroxidase oxidation within the pH range of 3.7--9.0 were studied. Both substituted imidazoles activate the reaction at less than 0.6. In the presence of 4-nitroimidazole the activation is non-competitive, whereas in the presence of benzimidazole it is of a mixed type, which is close to the non-competitive one. The kinetic parameters (kAcat, alpha, KA) for the reaction activated by both imidazoles were determined. It was assumed that the activators interact with the protein group (pK approximately to 6.5), which limits the enzyme activity. This results in the increase of pKapp of the protein group in question, resulting in the appearance of the maximal peroxidase activity in the alkaline region of pH. It was shown that the intermolecular interactions involved in the peroxidase-induced oxidative catalysis are largely due to electrostatic rather than to hydrophobic factors. | [Kinetics and mechanism of nucleophilic effect on the oxidation of o-dianisidine catalyzed by horseradish peroxidase]. The effects of benzimidazole and 4-nitroimidazole on the reaction of o-dianisidine peroxidase oxidation within the pH range of 3.7--9.0 were studied. Both substituted imidazoles activate the reaction at less than 0.6. In the presence of 4-nitroimidazole the activation is non-competitive, whereas in the presence of benzimidazole it is of a mixed type, which is close to the non-competitive one. The kinetic parameters (kAcat, alpha, KA) for the reaction activated by both imidazoles were determined. It was assumed that the activators interact with the protein group (pK approximately to 6.5), which limits the enzyme activity. This results in the increase of pKapp of the protein group in question, resulting in the appearance of the maximal peroxidase activity in the alkaline region of pH. It was shown that the intermolecular interactions involved in the peroxidase-induced oxidative catalysis are largely due to electrostatic rather than to hydrophobic factors. | [
-0.10003378242254257,
-0.01966135762631893,
-0.04698300361633301,
-0.007960437797009945,
0.02248566970229149,
-0.07604606449604034,
0.02212892286479473,
0.023088589310646057,
-0.06059447303414345,
-0.03489932045340538,
-0.01216974575072527,
0.03936454653739929,
-0.050747133791446686,
0.02398102357983589,
-0.007758758962154388,
-0.06549856066703796,
-0.0066122324205935,
0.004299267660826445,
-0.10408008098602295,
-0.00846592616289854,
-0.06763181835412979,
-0.0035755846183747053,
0.0419597327709198,
0.0289683286100626,
0.06944458186626434,
0.053884752094745636,
0.002674784744158387,
0.05150411278009415,
-0.06098761409521103,
-0.207079216837883,
0.02463916316628456,
-0.03019372560083866,
-0.014870394952595234,
-0.06940151005983353,
-0.017705252394080162,
0.03715743497014046,
-0.014019750989973545,
-0.025749173015356064,
-0.016801422461867332,
0.01086339633911848,
0.056714531034231186,
0.008785390295088291,
-0.010334143415093422,
0.05577286705374718,
0.010770522058010101,
-0.000717085727956146,
-0.03452197089791298,
0.033140379935503006,
0.05031243711709976,
-0.020426500588655472,
0.007604536600410938,
0.0021292869932949543,
0.0045895702205598354,
0.006684069521725178,
0.025928443297743797,
-0.0018454000819474459,
-0.011835508048534393,
0.06449897587299347,
-0.026249898597598076,
0.04643572121858597,
0.06915450096130371,
0.05283051356673241,
-0.18300248682498932,
0.04496542736887932,
0.07008227705955505,
-0.003868798026815057,
0.0511021725833416,
-0.012801829725503922,
0.020531602203845978,
0.06732603162527084,
-0.05515844747424126,
0.043950650840997696,
-0.014962404035031796,
-0.03879215568304062,
-0.019295046105980873,
0.003031241474673152,
-0.035426340997219086,
-0.05276774615049362,
-0.03681595250964165,
-0.007486884482204914,
-0.01677262783050537,
0.005023328121751547,
-0.05009496584534645,
-0.03369620814919472,
0.005352680571377277,
0.03914463147521019,
0.007769471034407616,
-0.03964998573064804,
0.016588274389505386,
0.04318089038133621,
0.010531529784202576,
-0.027437377721071243,
-0.03312893956899643,
-0.07542344182729721,
-0.025362541899085045,
0.11298288404941559,
0.01952567882835865,
0.013620550744235516,
-0.028829043731093407,
0.3879135847091675,
-0.007548254914581776,
0.04064882546663284,
-0.058858249336481094,
0.005303279496729374,
-0.03530186414718628,
-0.0010205270955339074,
-0.017614051699638367,
-0.03712297976016998,
0.047751445323228836,
0.02109408937394619,
0.02143758162856102,
-0.055663205683231354,
0.008170085027813911,
-0.01259895320981741,
-0.0009266347042284906,
0.01938372291624546,
0.017328305169939995,
-0.004440873861312866,
-0.01440400816500187,
0.02322559431195259,
0.004519703332334757,
-0.05253792181611061,
0.004243810661137104,
0.0748986303806305,
0.0315832793712616,
0.010808846913278103,
-0.02403203211724758,
0.09359177947044373,
-0.005325716454535723,
0.03358533978462219,
0.06946678459644318,
0.012790475971996784,
-0.02550233155488968,
0.021523412317037582,
-0.027983911335468292,
-0.012786787003278732,
-0.005344322416931391,
-0.02740439772605896,
0.02549402415752411,
0.027137644588947296,
-0.028128309175372124,
-0.06394987553358078,
-0.10484477877616882,
-0.05098740756511688,
-0.032085441052913666,
0.05852296203374863,
0.014727958478033543,
0.04512990638613701,
-0.00949783157557249,
-0.02153455838561058,
-0.021141143515706062,
0.06646011769771576,
-0.04615873843431473,
0.09153057634830475,
-0.061176594346761703,
-0.017716294154524803,
-0.01807471364736557,
-0.0341457761824131,
-0.016574406996369362,
0.03950977325439453,
-0.14055855572223663,
-0.04682605713605881,
0.030403228476643562,
0.08499355614185333,
-0.03642205893993378,
-0.015299907885491848,
-0.013568258844316006,
0.03417154401540756,
-0.01181743759661913,
0.04473013058304787,
-0.009574192576110363,
0.0049518924206495285,
0.013486558571457863,
-0.02253541350364685,
-0.035827238112688065,
0.026406975463032722,
0.06693007051944733,
0.05219045281410217,
0.02519906312227249,
-0.00041156227234750986,
0.043413273990154266,
0.019350342452526093,
-0.000277769286185503,
0.0740547776222229,
0.020632827654480934,
-0.0008415587362833321,
-0.06194588541984558,
0.04091019555926323,
0.05120616406202316,
-0.0032602790743112564,
-0.05246089771389961,
-0.015464101918041706,
-0.09524521231651306,
0.0629940778017044,
-0.023177841678261757,
-0.024270348250865936,
-0.02470613457262516,
0.01147123146802187,
0.037979789078235626,
-0.07009760290384293,
0.12632204592227936,
0.033253371715545654,
0.02201186679303646,
0.046224888414144516,
0.01471008826047182,
-0.013436120934784412,
0.02195519022643566,
0.03716862201690674,
-0.015433638356626034,
-0.07054034620523453,
0.003128642914816737,
-0.02971985749900341,
-0.04548124596476555,
0.004586643539369106,
-0.04061377793550491,
0.05900660902261734,
-0.025669122114777565,
0.031246567144989967,
0.05044619366526604,
0.008089575916528702,
0.06353649497032166,
-0.05704094097018242,
-0.0007958097849041224,
-0.2704247236251831,
-0.03161413595080376,
-0.014789574779570103,
0.006994944531470537,
0.00022151977464091033,
-0.05172615498304367,
0.0001378407614538446,
-0.05222382768988609,
-0.007177256979048252,
-0.024702882394194603,
0.06071081385016441,
0.0007410766556859016,
-0.038760267198085785,
-0.034252654761075974,
-0.020104125142097473,
0.041444625705480576,
-0.0075641535222530365,
-0.06827938556671143,
0.03590849041938782,
-0.014666186645627022,
0.0479399710893631,
-0.05231333151459694,
0.04027503728866577,
-0.01217969972640276,
0.04797670990228653,
-0.009483441710472107,
0.06370235979557037,
0.04152622073888779,
0.03195919841527939,
0.02587798424065113,
-0.0032953645568341017,
0.029168423265218735,
-0.05033819377422333,
-0.0832945704460144,
-0.005923583637923002,
-0.07302677631378174,
0.05297349393367767,
-0.07532397657632828,
0.026324152946472168,
-0.0046781026758253574,
-0.024148177355527878,
-0.0021756128408014774,
-0.001177717698737979,
-0.0894755944609642,
-0.0017878323560580611,
0.012132636271417141,
-0.017322491854429245,
0.025441352277994156,
0.0057008652947843075,
0.06555373221635818,
0.044651683419942856,
0.046124301850795746,
0.04679790511727333,
0.020464804023504257,
-0.004237344488501549,
-0.03667552396655083,
-0.07360448688268661,
0.04192330315709114,
-0.025382669642567635,
-0.003103602211922407,
0.008142978884279728,
-0.07255115360021591,
-0.02980353683233261,
0.03874025493860245,
-0.010507411323487759,
0.010965638794004917,
0.0038646021857857704,
-0.01950247772037983,
0.05377734452486038,
0.044734880328178406,
0.00380818173289299,
0.12051039934158325,
-0.044618334621191025,
0.0009088986553251743,
0.09933885186910629,
0.03313073888421059,
-0.04066332057118416,
0.024522220715880394,
-0.029538234695792198,
0.020117931067943573,
0.010142308659851551,
0.004732306580990553,
0.017565662041306496,
-0.001228829612955451,
-0.04831574857234955,
-0.01536401268094778,
0.035588111728429794,
0.0032710081432014704,
0.006857501808553934,
0.020091388374567032,
0.06082440912723541,
0.03768979012966156,
-0.030458834022283554,
0.009967358782887459,
0.03299248218536377,
0.009027649648487568,
-0.25772297382354736,
-0.006374062970280647,
0.03062821738421917,
0.01891372539103031,
-0.028677498921751976,
0.04868581146001816,
0.04118559509515762,
-0.06848488003015518,
-0.03712792694568634,
0.008798090741038322,
-0.0030177272856235504,
0.019106019288301468,
0.026495764032006264,
0.04447876662015915,
0.0074699511751532555,
0.006747107487171888,
0.044807299971580505,
-0.029205068945884705,
0.030837245285511017,
-0.022548561915755272,
0.03671598806977272,
0.002936557400971651,
0.13188950717449188,
-0.00917833112180233,
0.0137689970433712,
0.06909634172916412,
0.007671329192817211,
0.05102699622511864,
-0.02197275497019291,
-0.012192529626190662,
0.027075270190835,
0.01987631991505623,
0.058491844683885574,
-0.026999643072485924,
-0.014151177369058132,
-0.01154419220983982,
-0.004762559197843075,
0.040921930223703384,
-0.044691815972328186,
-0.0036708414554595947,
-0.02244235947728157,
-0.044705964624881744,
-0.09769090265035629,
-0.040469519793987274,
0.050773195922374725,
-0.015484824776649475,
-0.06875991821289062,
-0.040583740919828415,
-0.006032342091202736,
-0.01652645878493786,
-0.0198533795773983,
-0.06364430487155914,
0.013927286490797997,
0.03417814522981644,
0.026099735870957375,
0.03320365026593208,
-0.019519833847880363,
-0.00004205104778520763,
0.044691722840070724,
0.01732301339507103,
0.02572386898100376,
0.047912079840898514,
-0.03345436975359917,
0.01613975130021572,
0.023185579106211662
] |
PMID:27246 | [Properties of cyclic nucleotide phosphodiesterase from lingual taste papillae]. | Phosphodiesterase activity is estimated in extracts and partially purified preparations from functionally different parts of bovine tongue. The enzyme activity varied from 4.0 to 10.4 nmole/mg of protein/min. Properties of phosphodiesterase from circumvallate papillae are studied, the pH optimum being 8.0--8.5, Km for cAMP--1.5.10(-4) M and for cGMP--6.5.10(-5) M. The enzyme activity did not change after the treatment with trypsin, protamine sulphate (0.01--1.0%), heparin (0.01--1.0) and taste agents: L-leucine (from 1.10(-2) M to 1.10(-5) M), quinine (from 4.10(-3) M to 4.10(-8) M) and D-glucose (from 1.10(-1) M to 1.10(-4) M). The protein inhibitor of the enzyme, isolated from retina external rod-cell segments considerably suppressed phosphodiesterase activity, and the protein activator from brain tissue stimulated it insignificantly. Thermostable protein modulators, which inhibit or activate (depending on experimental conditions) phosphodiesterase activity, are isolated from circumvallate papillae. | [Properties of cyclic nucleotide phosphodiesterase from lingual taste papillae]. Phosphodiesterase activity is estimated in extracts and partially purified preparations from functionally different parts of bovine tongue. The enzyme activity varied from 4.0 to 10.4 nmole/mg of protein/min. Properties of phosphodiesterase from circumvallate papillae are studied, the pH optimum being 8.0--8.5, Km for cAMP--1.5.10(-4) M and for cGMP--6.5.10(-5) M. The enzyme activity did not change after the treatment with trypsin, protamine sulphate (0.01--1.0%), heparin (0.01--1.0) and taste agents: L-leucine (from 1.10(-2) M to 1.10(-5) M), quinine (from 4.10(-3) M to 4.10(-8) M) and D-glucose (from 1.10(-1) M to 1.10(-4) M). The protein inhibitor of the enzyme, isolated from retina external rod-cell segments considerably suppressed phosphodiesterase activity, and the protein activator from brain tissue stimulated it insignificantly. Thermostable protein modulators, which inhibit or activate (depending on experimental conditions) phosphodiesterase activity, are isolated from circumvallate papillae. | [
-0.05504848435521126,
-0.0033078535925596952,
-0.046390075236558914,
0.019442565739154816,
-0.03511052206158638,
-0.016042152419686317,
0.07525820285081863,
0.04762734845280647,
-0.06425324082374573,
-0.015158229507505894,
0.021428830921649933,
-0.07074292749166489,
-0.03789936751127243,
-0.013636460527777672,
-0.022459574043750763,
-0.017479706555604935,
0.050735943019390106,
-0.027751630172133446,
-0.04261644184589386,
-0.01820210926234722,
0.03543040528893471,
0.02621258795261383,
0.036087438464164734,
-0.018045444041490555,
0.009851626120507717,
0.033155590295791626,
0.04573187232017517,
0.05825500562787056,
-0.02258686162531376,
-0.19281622767448425,
0.021406911313533783,
-0.02555260807275772,
0.028178924694657326,
-0.05975637584924698,
-0.024643709883093834,
0.03668588772416115,
-0.056958746165037155,
0.046766508370637894,
0.009228014387190342,
0.05980264022946358,
0.10015749931335449,
0.011520878411829472,
-0.013274827040731907,
0.02926340512931347,
0.042725030332803726,
-0.0532541498541832,
-0.08223158866167068,
-0.016293689608573914,
0.08774665743112564,
0.03698251396417618,
0.02795715257525444,
-0.043953005224466324,
0.04889671877026558,
0.0014548501931130886,
-0.024049561470746994,
-0.0031580128706991673,
0.0030241976492106915,
0.03663570061326027,
-0.002935680327937007,
-0.0017979855183511972,
0.009800327941775322,
0.036826252937316895,
-0.12702445685863495,
0.056725889444351196,
0.019112640991806984,
-0.020069975405931473,
0.0028216491919010878,
-0.029504986479878426,
-0.005934508983045816,
0.05041539669036865,
-0.07576826959848404,
0.05099513381719589,
0.0012221469078212976,
-0.05053425952792168,
0.0045877667143940926,
-0.0043291570618748665,
0.003943365067243576,
0.036947429180145264,
0.044907256960868835,
0.024477778002619743,
0.0026717206928879023,
0.0069692302495241165,
0.003094966057687998,
-0.02943616546690464,
0.020145300775766373,
0.031550634652376175,
-0.0008367736591026187,
0.018916234374046326,
-0.027187978848814964,
0.04339862987399101,
-0.04704824462532997,
-0.07887177169322968,
-0.05242455378174782,
-0.06573083996772766,
-0.035484157502651215,
0.12476892024278641,
0.036462120711803436,
-0.006707060616463423,
-0.026087304577231407,
0.3790600299835205,
-0.01690274477005005,
0.011581292375922203,
-0.09594820439815521,
-0.04484979063272476,
0.007715826388448477,
-0.018869532272219658,
-0.006636015605181456,
0.013350228779017925,
-0.041089434176683426,
0.08520819991827011,
0.03671221062541008,
0.03912048414349556,
-0.002596338978037238,
-0.05542656406760216,
-0.01865018717944622,
-0.028636816889047623,
0.011735930107533932,
0.018118876963853836,
0.047460928559303284,
0.07795639336109161,
-0.047458525747060776,
0.0054479907266795635,
0.04382374882698059,
-0.013757545500993729,
0.05872991681098938,
0.006464424543082714,
-0.0256313756108284,
0.10050708055496216,
0.040737759321928024,
0.028435543179512024,
0.01446398813277483,
0.02967623434960842,
0.04827820509672165,
0.007738508749753237,
0.03074292652308941,
-0.02441396564245224,
0.02171018160879612,
-0.022102411836385727,
0.058378394693136215,
0.016106491908431053,
0.005208409391343594,
-0.09193646162748337,
-0.0007248366600833833,
-0.0645996704697609,
-0.0004113516479264945,
0.06957988440990448,
-0.007138990331441164,
0.03321035951375961,
-0.017025014385581017,
0.045040279626846313,
0.014117035083472729,
0.043438754975795746,
-0.04346887767314911,
0.030207158997654915,
0.034816574305295944,
0.037162140011787415,
-0.05461099371314049,
-0.02065371535718441,
0.03983350843191147,
0.0007144391420297325,
-0.11339318752288818,
0.0007219793042168021,
0.05363752692937851,
0.04562375321984291,
-0.010556947439908981,
-0.017102075740695,
-0.06563027203083038,
-0.003094228683039546,
0.006466537248343229,
-0.02784218080341816,
-0.009764686226844788,
-0.006121625192463398,
-0.06981615722179413,
-0.05022916942834854,
-0.04366059601306915,
0.030034970492124557,
0.03456132486462593,
-0.029809951782226562,
0.022022971883416176,
-0.004977656994014978,
0.004727344959974289,
-0.029225917533040047,
-0.018892984837293625,
-0.009171671234071255,
0.027662649750709534,
-0.010606182739138603,
-0.012096856720745564,
-0.03627868741750717,
0.020176490768790245,
0.05732756108045578,
-0.009440915659070015,
-0.006810028571635485,
-0.041895542293787,
0.0511099174618721,
-0.06289009749889374,
-0.04893752187490463,
-0.039984870702028275,
0.03184190392494202,
-0.009028223343193531,
-0.02368212677538395,
0.09872163087129593,
0.017982425168156624,
0.0007748515927232802,
-0.009805107489228249,
0.04502691328525543,
0.032407306134700775,
-0.016334157437086105,
0.07470865547657013,
0.06924314051866531,
-0.006519571412354708,
-0.03449386730790138,
0.0026467745192348957,
0.012975413352251053,
-0.004698487464338541,
-0.04808032512664795,
0.0016529239946976304,
-0.00820494070649147,
0.03904605284333229,
0.03907809033989906,
-0.007482324261218309,
-0.028137633576989174,
-0.05840827897191048,
-0.060664184391498566,
-0.30125531554222107,
-0.05795616656541824,
0.04141147434711456,
0.013697553426027298,
0.07656488567590714,
-0.015841854736208916,
0.041424937546253204,
-0.03312758356332779,
0.044160857796669006,
-0.04713776707649231,
0.04056146740913391,
0.08353564143180847,
-0.05529570206999779,
0.0015856924001127481,
-0.011842161417007446,
0.061365433037281036,
0.06807541102170944,
-0.03438370302319527,
0.020083870738744736,
0.005964552517980337,
0.04553501307964325,
-0.04179277643561363,
-0.0013255377998575568,
-0.02735714241862297,
0.01532821450382471,
0.010617604479193687,
0.0671674832701683,
0.008896800689399242,
-0.02629615180194378,
-0.028463099151849747,
0.04199216514825821,
0.06410510838031769,
-0.011227928102016449,
-0.10268912464380264,
-0.02495042234659195,
0.013722531497478485,
-0.003607079153880477,
0.0039552911184728146,
0.0031286366283893585,
0.00936647318303585,
-0.0466291606426239,
0.047466911375522614,
0.002182689495384693,
-0.04571439325809479,
-0.06420352309942245,
-0.01198560744524002,
0.03439953178167343,
0.02357099950313568,
0.00574216153472662,
-0.023153919726610184,
0.02431461028754711,
-0.003133723046630621,
0.05623457580804825,
-0.013129029422998428,
-0.010023495182394981,
-0.03684819117188454,
-0.035646796226501465,
0.0017134486697614193,
-0.08376064896583557,
-0.021413195878267288,
0.02554529532790184,
0.009463715367019176,
-0.01919039525091648,
0.011440861970186234,
-0.013096886686980724,
0.029445156455039978,
0.043419428169727325,
-0.02015722543001175,
0.05589819699525833,
0.03336075693368912,
0.03168095275759697,
0.0679047480225563,
-0.05242215096950531,
0.0113443061709404,
0.011523783206939697,
-0.0148731404915452,
-0.01193356141448021,
-0.022400707006454468,
-0.07954639196395874,
0.014407905749976635,
-0.0085105886682868,
0.010908316820859909,
0.06351805478334427,
-0.05132234841585159,
-0.05684874579310417,
0.026492342352867126,
0.024117767810821533,
-0.06969250738620758,
0.049226049333810806,
-0.01034779567271471,
0.047689516097307205,
0.008458280935883522,
-0.03284870833158493,
-0.05326852947473526,
0.03900301828980446,
0.026720495894551277,
-0.2770490348339081,
-0.0260916780680418,
0.015178672969341278,
-0.001021791948005557,
-0.016195006668567657,
0.04068663716316223,
0.01682530902326107,
-0.09214194118976593,
-0.014960100874304771,
0.024027720093727112,
0.05274764820933342,
0.025971295312047005,
0.06254323571920395,
0.020630856975913048,
-0.019355574622750282,
-0.010227068327367306,
0.02460213378071785,
0.0035601542331278324,
0.029129670932888985,
-0.058657206594944,
0.037748176604509354,
-0.03482207655906677,
0.15550336241722107,
-0.0026711500249803066,
-0.023601191118359566,
0.0018890639767050743,
0.02955923043191433,
0.00006569419201696292,
-0.04370853304862976,
-0.020100265741348267,
-0.07231313735246658,
-0.004933907650411129,
0.11126207560300827,
0.010897326283156872,
0.00486243749037385,
0.06635583192110062,
-0.043568965047597885,
0.0037373662926256657,
-0.030447721481323242,
-0.027899956330657005,
-0.026320450007915497,
0.02464338205754757,
-0.06627680361270905,
-0.04637133330106735,
0.03603091090917587,
-0.10620223730802536,
0.005661528557538986,
-0.026435112580657005,
0.014736322686076164,
-0.015545135363936424,
-0.006014667451381683,
-0.07471945881843567,
0.03323844075202942,
-0.029607607051730156,
-0.021818023175001144,
-0.00004279131462681107,
-0.028701301664114,
-0.01882137730717659,
0.03610965609550476,
0.03027895838022232,
-0.00368476496078074,
-0.018201589584350586,
0.04897983372211456,
0.0886768028140068,
0.03834832087159157
] |
PMID:27247 | [Coenzyme-induced slow transitions of NADP-sorbitol dehydrogenase from Gluconobacter oxydans]. | The kinetic properties of NADP-dependent sorbitol dehydrogenase from G. oxydans cell extract were studied at pH 8.8 and 9.3 in the direction of D-sorbitol oxydation. It was shown that the shape of the kinetic curves of NADPH accumulation in time is characterised by initial burst whose magnitude depends on the concentration of the enzyme extract used. Preincubation of the enzyme with NADP or D-sorbitol eliminated the initial burst on these curves and transformed them into straight lines coming from the start of co-ordinates. The dependence of the stationary reaction rate on the enzyme extract concentration is not a linear one. The kinetic dependences of stationary rate of the reaction catalysed by the enzyme on the concentration of D-sorbitol and NADP at pH 8.8 and 9.3 were examined under all conditions studied; the shape of these kinetic curves altered to considerable extent with the alteration of the enzyme extract concentration in the reaction mixture and pH. At pH 9.3 several intermiediate plateaux were found on the curves of the D-sorbitol concentration dependent stationary rate of the reaction. The preincubation of the enzyme extract with NADP during 1.5 h removed the intermediate plateau on these curves and made them hyperbolic. Disk-electrophoresis of the enzyme extract in PAAG concentration gradient showed that at pH 8.8 the enzyme exists in one active form, while at pH 9.3 it exists in three major and three minor active forms of the enzyme differing in their molecular weights are found. It is assumed that the enzyme from G. oxydans cell extract can exist in a great number of molecular equilibrium forms, the rate of quilibrium being comparable or significantly less than that of the enzymatic reaction. NADP significantly influences on the equilibrium of the molecular forms of the enzyme. | [Coenzyme-induced slow transitions of NADP-sorbitol dehydrogenase from Gluconobacter oxydans]. The kinetic properties of NADP-dependent sorbitol dehydrogenase from G. oxydans cell extract were studied at pH 8.8 and 9.3 in the direction of D-sorbitol oxydation. It was shown that the shape of the kinetic curves of NADPH accumulation in time is characterised by initial burst whose magnitude depends on the concentration of the enzyme extract used. Preincubation of the enzyme with NADP or D-sorbitol eliminated the initial burst on these curves and transformed them into straight lines coming from the start of co-ordinates. The dependence of the stationary reaction rate on the enzyme extract concentration is not a linear one. The kinetic dependences of stationary rate of the reaction catalysed by the enzyme on the concentration of D-sorbitol and NADP at pH 8.8 and 9.3 were examined under all conditions studied; the shape of these kinetic curves altered to considerable extent with the alteration of the enzyme extract concentration in the reaction mixture and pH. At pH 9.3 several intermiediate plateaux were found on the curves of the D-sorbitol concentration dependent stationary rate of the reaction. The preincubation of the enzyme extract with NADP during 1.5 h removed the intermediate plateau on these curves and made them hyperbolic. Disk-electrophoresis of the enzyme extract in PAAG concentration gradient showed that at pH 8.8 the enzyme exists in one active form, while at pH 9.3 it exists in three major and three minor active forms of the enzyme differing in their molecular weights are found. It is assumed that the enzyme from G. oxydans cell extract can exist in a great number of molecular equilibrium forms, the rate of quilibrium being comparable or significantly less than that of the enzymatic reaction. NADP significantly influences on the equilibrium of the molecular forms of the enzyme. | [
-0.09411367774009705,
0.009092657826840878,
-0.01838199980556965,
-0.01400849036872387,
0.042251136153936386,
-0.00998146552592516,
0.019456498324871063,
-0.017877014353871346,
-0.03748135268688202,
-0.07295941561460495,
0.040998462587594986,
-0.07057248055934906,
0.006423850078135729,
-0.020202340558171272,
-0.04567781835794449,
-0.00311332568526268,
0.027142679318785667,
-0.006492272950708866,
-0.050100937485694885,
-0.00044639952830038965,
0.009411504492163658,
-0.015545002184808254,
0.030440544709563255,
0.03427799791097641,
0.05835533142089844,
0.038051947951316833,
0.03373430669307709,
0.03199689835309982,
-0.05941459536552429,
-0.21544212102890015,
0.02484229765832424,
-0.008997166529297829,
0.02346648834645748,
-0.054262734949588776,
-0.028394270688295364,
0.05206397920846939,
0.001069757156074047,
0.031276680529117584,
0.0023622734006494284,
-0.029864130541682243,
-0.024784594774246216,
0.028913235291838646,
-0.023294763639569283,
0.009792525321245193,
-0.011634464375674725,
-0.004478875547647476,
-0.03795301914215088,
0.06455416232347488,
0.03320049121975899,
0.0056977663189172745,
-0.04857863485813141,
-0.002894561504945159,
0.015371717512607574,
-0.009384425356984138,
0.0495079942047596,
-0.028461221605539322,
0.022430991753935814,
0.01809750869870186,
-0.010126358829438686,
-0.03424462676048279,
-0.003297569463029504,
-0.01010877825319767,
-0.13585567474365234,
0.017825905233621597,
0.06667709350585938,
-0.016602372750639915,
-0.004796871915459633,
0.006077203433960676,
0.08836155384778976,
0.017974505200982094,
-0.07167582213878632,
0.03300042450428009,
-0.0005682912305928767,
-0.009892587549984455,
0.006678326986730099,
-0.01781574822962284,
0.008730298839509487,
0.010177087970077991,
0.03374659642577171,
0.015059151686728,
-0.014715861529111862,
-0.002843176946043968,
-0.01778366230428219,
-0.005872718058526516,
-0.0458252876996994,
0.024432649835944176,
0.06092141568660736,
-0.050929974764585495,
0.011813512071967125,
0.040071140974760056,
0.005047441925853491,
-0.05279648303985596,
0.002630773698911071,
0.001451537711545825,
-0.12189151346683502,
0.05804150924086571,
0.03713013604283333,
0.015194177627563477,
0.07367392629384995,
0.40258464217185974,
-0.025599835440516472,
0.008927359245717525,
-0.04334769397974014,
-0.025991708040237427,
0.0066628665663301945,
-0.07866484671831131,
-0.027091724798083305,
0.03451421856880188,
0.07030031085014343,
0.06588194519281387,
0.048224207013845444,
0.020057078450918198,
0.004836392588913441,
-0.11186045408248901,
0.027479007840156555,
0.004420192912220955,
0.021383007988333702,
0.004354669246822596,
-0.0543065182864666,
0.05254241079092026,
0.009915472008287907,
0.02664036676287651,
-0.021273920312523842,
0.026583926752209663,
0.04458913952112198,
0.006712723057717085,
-0.03667353093624115,
0.09576011449098587,
0.049936555325984955,
-0.012230011634528637,
0.058258093893527985,
0.013628820888698101,
-0.0008591843652538955,
0.018421441316604614,
-0.0006914279074408114,
0.003273910842835903,
0.04031812772154808,
-0.006680663209408522,
-0.0006395815289579332,
-0.058351557701826096,
-0.011420500464737415,
-0.02421589381992817,
-0.02195998653769493,
-0.05570167303085327,
-0.061102934181690216,
0.06946805864572525,
0.01039255689829588,
0.01745445281267166,
-0.006716686300933361,
-0.00971280038356781,
-0.015681441873311996,
0.039428722113370895,
-0.013662523590028286,
0.008938920684158802,
-0.004748727660626173,
0.06687992811203003,
-0.03938750550150871,
-0.016682913526892662,
0.005652888678014278,
-0.04445631429553032,
-0.11949179321527481,
-0.005771372001618147,
-0.007037839852273464,
0.0965375006198883,
0.007621879689395428,
0.0027709617279469967,
-0.032821185886859894,
0.004025307018309832,
0.019386589527130127,
0.000058701658417703584,
-0.007111208979040384,
-0.003015008056536317,
-0.013712834566831589,
-0.062015634030103683,
-0.0019105024402961135,
0.03071170672774315,
0.020436067134141922,
0.05238514766097069,
0.04933419078588486,
-0.03623269870877266,
0.03184328228235245,
0.026907339692115784,
-0.012468168511986732,
-0.02532830461859703,
0.08024430274963379,
0.03778811916708946,
-0.05680248886346817,
0.0669374093413353,
0.006533857900649309,
0.018213417381048203,
-0.019154304638504982,
0.0018864594167098403,
-0.10777375102043152,
0.024212438613176346,
-0.018063506111502647,
-0.05058179050683975,
-0.011093606241047382,
-0.009673683904111385,
-0.012920083478093147,
-0.007200115825980902,
0.09294022619724274,
0.016535978764295578,
0.001010435400530696,
-0.004088391549885273,
0.004642934538424015,
-0.03765605390071869,
0.008963118307292461,
0.020128725096583366,
0.02120610885322094,
0.015152174048125744,
-0.0792885571718216,
-0.02635796368122101,
-0.09766913205385208,
-0.00008885525312507525,
-0.05643410235643387,
0.054522398859262466,
-0.01168101467192173,
0.011729279533028603,
0.01047531608492136,
0.015292169526219368,
0.00682136882096529,
-0.05951741710305214,
-0.031441159546375275,
-0.3278541564941406,
0.008318917825818062,
0.02184249274432659,
-0.030799971893429756,
0.06193188205361366,
-0.019754216074943542,
0.04334283992648125,
-0.0220956951379776,
0.011050793342292309,
-0.038550350815057755,
0.06149943545460701,
0.03221598640084267,
-0.04318144917488098,
-0.056993063539266586,
-0.0169991347938776,
-0.011936015449464321,
0.036742061376571655,
0.014174062758684158,
0.02941320464015007,
0.03371170908212662,
0.015572919510304928,
-0.03555363416671753,
0.027648383751511574,
-0.012649969197809696,
0.03248374164104462,
-0.05575168505311012,
0.10248170047998428,
-0.011113561689853668,
-0.002495399909093976,
-0.004998939577490091,
-0.0008728496031835675,
0.012748293578624725,
-0.01789434440433979,
-0.094398133456707,
-0.03494105115532875,
-0.008027064613997936,
0.032911159098148346,
-0.0801980271935463,
0.07848427444696426,
-0.0046351454220712185,
0.006114649120718241,
0.049429044127464294,
0.020134707912802696,
-0.03706620633602142,
-0.012850267812609673,
-0.003113206708803773,
0.024330709129571915,
0.013059130869805813,
-0.02196495607495308,
-0.02496129833161831,
0.013361260294914246,
-0.0027535471599549055,
0.057606615126132965,
-0.04246808588504791,
-0.020497355610132217,
0.028318269178271294,
-0.010847561992704868,
0.05098135396838188,
-0.06778490543365479,
0.02008959650993347,
-0.04236586391925812,
0.039551980793476105,
-0.019188260659575462,
0.04419749975204468,
0.055276818573474884,
0.036407891660928726,
-0.0534650981426239,
-0.029123421758413315,
0.015089789405465126,
-0.02050173096358776,
0.016581309959292412,
0.10867385566234589,
-0.04178326204419136,
0.03868747130036354,
0.019128846004605293,
0.03771563991904259,
-0.04710254445672035,
-0.01035492867231369,
-0.05535586550831795,
0.00823768600821495,
0.007838101126253605,
-0.05498775467276573,
0.09363830834627151,
0.008690192364156246,
-0.003958129324018955,
0.03247193247079849,
0.04773649200797081,
-0.06606919318437576,
-0.008870955556631088,
-0.02115681581199169,
0.015189516358077526,
0.0032149760518223047,
-0.006385634187608957,
-0.014894014224410057,
0.03422734513878822,
0.033751070499420166,
-0.2730603814125061,
0.00014833266322966665,
-0.018398741260170937,
0.022851871326565742,
0.005608230363577604,
0.046055495738983154,
0.06964435428380966,
-0.03126262128353119,
-0.018679173663258553,
0.03790920600295067,
-0.007991839200258255,
0.03037145361304283,
0.0123463599011302,
0.00790505763143301,
0.04849102348089218,
-0.00446627102792263,
0.007880263030529022,
-0.03954184800386429,
0.04575663432478905,
-0.039530735462903976,
0.00408846465870738,
-0.030761227011680603,
0.13774339854717255,
-0.01088402234017849,
0.02263406664133072,
0.02773328311741352,
-0.02746468223631382,
0.04155433550477028,
-0.017586825415492058,
-0.02307620272040367,
-0.0030064587481319904,
-0.0018489276990294456,
0.108026884496212,
-0.06035606190562248,
-0.007782332599163055,
0.06379122287034988,
-0.015158010646700859,
0.03639598563313484,
0.008457554504275322,
0.02352307364344597,
0.017875634133815765,
0.01189474668353796,
-0.06389977037906647,
0.011332837864756584,
0.05101827159523964,
-0.11380904167890549,
-0.05413176119327545,
-0.035583943128585815,
-0.011359800584614277,
0.08406206965446472,
-0.019667690619826317,
-0.024464484304189682,
0.035945843905210495,
0.00011025561252608895,
-0.008161397650837898,
0.0010280051501467824,
-0.006665379274636507,
0.012432632967829704,
-0.08180275559425354,
-0.02507001906633377,
0.022556589916348457,
0.006756252609193325,
-0.006230724975466728,
0.029814690351486206,
0.027273664250969887
] |
PMID:27248 | [Presence of SH-groups and histidine in the active site of Chlorella glutamine synthetase]. | The presence of two cysteine residues per each six monomers comprising the oligomer of Chlorella glutamine synthetase (E.C.6.3.1.2) is demonstrated using homogenous enzyme preparation. p-Chloromercuribenzoate (p-CMB) is found to inhibit glutamine synthetase activity, the degree of inhibition depending on the inhibitor concentration. The following enzyme reactivation by dithiotreitol (10(-2) M) was observed only when the enzyme was inactivated with 10(-5) M p-CMB under 15 min. preincubation. Preincubation of the enzyme with 10(-4) M p-CMB for 45 min. did not result in its reactivation. Gel filtration of glutamine synthetase treated with 10(-4) M p-CMB has revealed the dissociation of the enzyme into inactive monomers. Incubation of glutamine synthetase with p-CMB at various pH values, incubation after pre-treatment with urea and experiments with HgCl2 indicate the presence of free and masked inside the globula SH-groups in the enzyme molecule. Competitive character of the enzyme inhibition with p-CMB with respect to ATP indicates that SH-groups of the active site participate in the ATP binding, probably, as Mg-ATP or Mn-ATP complexes. Data on the estimation of ionization constant of glutamate-binding group and experiments on the effect of histidine photooxidation on the enzyme activity indicate the presence of histidine residue in the enzyme active site, which participates in glutamate binding. | [Presence of SH-groups and histidine in the active site of Chlorella glutamine synthetase]. The presence of two cysteine residues per each six monomers comprising the oligomer of Chlorella glutamine synthetase (E.C.6.3.1.2) is demonstrated using homogenous enzyme preparation. p-Chloromercuribenzoate (p-CMB) is found to inhibit glutamine synthetase activity, the degree of inhibition depending on the inhibitor concentration. The following enzyme reactivation by dithiotreitol (10(-2) M) was observed only when the enzyme was inactivated with 10(-5) M p-CMB under 15 min. preincubation. Preincubation of the enzyme with 10(-4) M p-CMB for 45 min. did not result in its reactivation. Gel filtration of glutamine synthetase treated with 10(-4) M p-CMB has revealed the dissociation of the enzyme into inactive monomers. Incubation of glutamine synthetase with p-CMB at various pH values, incubation after pre-treatment with urea and experiments with HgCl2 indicate the presence of free and masked inside the globula SH-groups in the enzyme molecule. Competitive character of the enzyme inhibition with p-CMB with respect to ATP indicates that SH-groups of the active site participate in the ATP binding, probably, as Mg-ATP or Mn-ATP complexes. Data on the estimation of ionization constant of glutamate-binding group and experiments on the effect of histidine photooxidation on the enzyme activity indicate the presence of histidine residue in the enzyme active site, which participates in glutamate binding. | [
-0.04533618688583374,
-0.021882900968194008,
-0.06433023512363434,
0.020046329125761986,
-0.0033790916204452515,
0.045046545565128326,
0.04243073612451553,
0.02261856198310852,
-0.08936876058578491,
0.016939884051680565,
0.016543827950954437,
-0.07769455760717392,
0.04749660938978195,
0.026031354442238808,
-0.045986421406269073,
-0.033674098551273346,
-0.04897649213671684,
0.06325208395719528,
-0.015860550105571747,
-0.05019542574882507,
-0.017206912860274315,
0.026062767952680588,
0.020968543365597725,
-0.005843302235007286,
0.08339086920022964,
0.02249029092490673,
0.017926203086972237,
0.07332803308963776,
-0.033712126314640045,
-0.2268488109111786,
0.00498198252171278,
0.004167153500020504,
0.021408874541521072,
-0.07205729186534882,
-0.0030833957716822624,
0.041207581758499146,
-0.020932389423251152,
-0.0320369116961956,
-0.046518441289663315,
0.02375400811433792,
0.01345046702772379,
0.0010057413019239902,
0.06734514981508255,
0.033654820173978806,
-0.009067317470908165,
-0.007217133417725563,
-0.03702026605606079,
-0.0015207917895168066,
0.0892450213432312,
-0.014828420244157314,
0.006621228065341711,
-0.031647101044654846,
0.06897847354412079,
0.025364231318235397,
0.0027919276617467403,
0.021707642823457718,
0.031941723078489304,
0.03712465986609459,
0.015879333019256592,
-0.005229828879237175,
0.022014271467924118,
0.04092441499233246,
-0.13693805038928986,
0.03712066635489464,
0.07102885097265244,
-0.00031453039264306426,
0.023175427690148354,
-0.07764827460050583,
0.033124882727861404,
0.016316616907715797,
-0.07584468275308609,
-0.0005431914469227195,
-0.03269917890429497,
-0.01892893761396408,
0.04404471814632416,
-0.0037998901680111885,
0.013499424792826176,
0.026235472410917282,
0.012589463964104652,
0.042868152260780334,
-0.014543281868100166,
-0.01027037762105465,
0.018431633710861206,
-0.02392965368926525,
0.00472066318616271,
-0.009615769609808922,
-0.009517433121800423,
0.02638423815369606,
-0.003944217227399349,
0.029862672090530396,
-0.021781112998723984,
-0.11491437256336212,
0.04042937979102135,
-0.06258372962474823,
-0.07720247656106949,
0.04521502926945686,
0.026526274159550667,
0.011395391076803207,
-0.009243967942893505,
0.4051605463027954,
-0.026060504838824272,
0.0032139811664819717,
-0.09573213756084442,
0.029029855504631996,
0.03740615025162697,
-0.008491779677569866,
-0.010336176492273808,
0.02315768040716648,
0.07786151021718979,
0.03551030904054642,
0.0761096253991127,
0.019889049232006073,
0.014452468603849411,
-0.08823884278535843,
-0.02533283270895481,
0.027981596067547798,
0.011171374469995499,
0.007816883735358715,
-0.10048682242631912,
0.0342722125351429,
-0.0019008268136531115,
-0.026176251471042633,
0.015088682994246483,
0.025498002767562866,
0.043414156883955,
0.012460085563361645,
-0.07456568628549576,
0.0688273161649704,
0.0773269310593605,
-0.009306454099714756,
0.03881426155567169,
0.06945434957742691,
0.018864963203668594,
-0.005892203189432621,
-0.02783832512795925,
-0.005312005989253521,
0.05031677335500717,
-0.03592146188020706,
0.05538303777575493,
-0.001571233500726521,
-0.016635211184620857,
-0.03780931979417801,
-0.03779870644211769,
-0.05705024302005768,
-0.056707289069890976,
0.07504242658615112,
0.010909338481724262,
0.006040029227733612,
-0.006699447054415941,
-0.0013896155869588256,
-0.02627316489815712,
0.041088663041591644,
-0.016771741211414337,
0.007547742687165737,
-0.03479853644967079,
0.03636087104678154,
0.006212664768099785,
-0.0096398601308465,
0.03619387373328209,
0.031481627374887466,
-0.12491799145936966,
0.011928240768611431,
-0.01880531758069992,
0.07090963423252106,
-0.003358015324920416,
-0.0178365521132946,
-0.023713966831564903,
0.02276558056473732,
-0.05136589705944061,
-0.006911660078912973,
-0.01207663957029581,
0.08939900994300842,
0.00926002487540245,
-0.0185292549431324,
-0.020937373861670494,
0.018043259158730507,
0.05372866243124008,
0.012254919856786728,
0.012141109444200993,
0.024298451840877533,
0.018052641302347183,
0.02799971029162407,
-0.039069630205631256,
0.002415542956441641,
-0.008352866396307945,
-0.0030232116114348173,
-0.029630059376358986,
-0.001650667516514659,
-0.0006307993899099529,
0.018193747848272324,
-0.022068366408348083,
0.0072548771277070045,
-0.07700719684362411,
0.04133223369717598,
-0.026571398600935936,
-0.07081251591444016,
-0.04108971729874611,
0.000877384387422353,
0.029377594590187073,
-0.016287775710225105,
0.11624473333358765,
0.04231862723827362,
0.015323039144277573,
0.02022743783891201,
0.019359540194272995,
-0.029111485928297043,
-0.0066430093720555305,
0.018748683854937553,
0.029811667278409004,
0.0021733585745096207,
0.006767733953893185,
0.013955743052065372,
-0.03549938648939133,
-0.009056217968463898,
-0.03270119056105614,
-0.01499427855014801,
0.006290793884545565,
0.026439720764756203,
0.041304197162389755,
0.022951176390051842,
0.005656530614942312,
-0.021926194429397583,
-0.09479871392250061,
-0.29510751366615295,
-0.041889965534210205,
0.0024252980947494507,
-0.001417746883817017,
0.06991751492023468,
-0.06336978077888489,
0.008150743320584297,
-0.00947087723761797,
-0.009906969033181667,
-0.054393377155065536,
0.06104406714439392,
0.05073191225528717,
-0.010381624102592468,
-0.07089613378047943,
-0.006197130307555199,
-0.005879271309822798,
0.03842170536518097,
-0.02546810172498226,
0.040319379419088364,
-0.002055370481684804,
0.062438592314720154,
-0.022959547117352486,
-0.011560340411961079,
0.01854131557047367,
0.07285679876804352,
-0.0181757602840662,
0.0967312678694725,
0.0029372835997492075,
0.008189084939658642,
-0.026955699548125267,
-0.00624989066272974,
0.05389576777815819,
-0.040256746113300323,
-0.0757068321108818,
-0.041575368493795395,
0.015282104723155499,
-0.018616756424307823,
-0.04178989306092262,
0.026438377797603607,
-0.04365038126707077,
0.03935268521308899,
-0.006086094304919243,
-0.012220996432006359,
-0.09303507208824158,
-0.02854534424841404,
-0.016844607889652252,
-0.0006437715492211282,
0.019240843132138252,
0.06666003912687302,
-0.04327479004859924,
-0.024941815063357353,
0.018722550943493843,
0.033865973353385925,
0.019912080839276314,
-0.022849244996905327,
-0.00952244270592928,
-0.015639062970876694,
0.06517428904771805,
-0.021436940878629684,
0.0378422848880291,
0.002419960917904973,
0.04281098023056984,
0.014446303248405457,
0.044191088527441025,
-0.030007561668753624,
-0.07031355053186417,
-0.013509969227015972,
-0.07297071069478989,
0.04627690836787224,
0.04042290523648262,
0.029484964907169342,
0.06345109641551971,
-0.03385196626186371,
0.026876524090766907,
0.02056194841861725,
0.031084246933460236,
-0.02395876683294773,
-0.07905979454517365,
-0.06821933388710022,
-0.009847481735050678,
-0.018569597974419594,
0.02606865018606186,
0.06154143810272217,
-0.03259361535310745,
0.025131769478321075,
0.053110867738723755,
-0.0056449491530656815,
-0.017942175269126892,
0.023278696462512016,
-0.006063144654035568,
0.020876923575997353,
0.009285254403948784,
-0.061985261738300323,
-0.03524177148938179,
0.046297311782836914,
-0.006827551871538162,
-0.2567223608493805,
0.04879562556743622,
-0.04480215162038803,
-0.0032186636235564947,
-0.05571744590997696,
0.04255761578679085,
0.012807175517082214,
-0.038897983729839325,
-0.01647069863975048,
0.015548239462077618,
0.03590868413448334,
0.010701391845941544,
0.03257569298148155,
0.06229709833860397,
0.008072884753346443,
0.017423832789063454,
0.023056695237755775,
-0.02052394673228264,
0.03470456972718239,
-0.0346495695412159,
-0.012546749785542488,
-0.057365093380212784,
0.1420593559741974,
0.021589631214737892,
0.019855236634612083,
0.05541769042611122,
0.014038263820111752,
-0.03845931589603424,
-0.010712621733546257,
0.05109289288520813,
-0.040458325296640396,
0.017486779019236565,
0.11483606696128845,
-0.0350426584482193,
0.019797775894403458,
0.014245947822928429,
-0.006445821840316057,
-0.012269028462469578,
-0.017255565151572227,
-0.030788946896791458,
-0.015687789767980576,
0.018531709909439087,
-0.05802424997091293,
-0.03327442705631256,
0.08473007380962372,
-0.02186182141304016,
-0.04111965000629425,
-0.07409679889678955,
-0.028364716097712517,
0.020874418318271637,
-0.043825484812259674,
-0.04140812158584595,
-0.021057771518826485,
-0.0041784620843827724,
-0.026669414713978767,
-0.04863303154706955,
-0.06570670753717422,
0.002775853965431452,
-0.008513862267136574,
0.024226313456892967,
0.029698079451918602,
0.014756479300558567,
0.025460058823227882,
0.04008286073803902,
0.04011579230427742
] |
PMID:27249 | [Isolation, purification and characterization of regulatory properties of adenylate cyclase from rabbit heart]. | Rabbit heart membranes possessing the adenylate cyclase activity were isolated and purified by extraction with high ionic strength solutions and centrifugation in the sucrose density gradient. It was shown that the membranes are characterized by a high percentage of cholesterol (molar ratio cholesterol/phospholipids is 0.24) and an increased activity of Na, K-ATPase, which suggests the localization of adenylate cyclase in the sarcolemma. During centrifugation in the sucrose density gradient the activities of andenylate cyclase and Na,K-ATPase are not separated. Treatment of heart sarcolemma with a 0.3% solution of lubrol WX results in 10--20% solubilization of adenylate cyclase. Purification of the enzyme in the membrane fraction is accompanied by a decrease in the activity of phosphodiesterase; however, about 2% of the heart diesterase total activity cannot be removed from the sarcolemma even after its treatment with 0.3% lubrol WX. Epinephrine and NaF activate adenylate cyclase without changing the pH dependence of the enzyme. The alpha-adrenergic antagonist phentolamine has no effect on the adenylate cyclase activation by catecholamines, glucagon and histamine; the beta-adrenergic antagonist alprenolol competitively inhibits the effects of isoproterenol, epinephrine and norepinephrine, having no effect on the enzyme activation by glucagon and histamine. There is no competition between epinephrine, glucagon and histamine for the binding site of the hormone; however, there may occur a competition between the hormone receptors for the binding to the enzyme. A combined action of several hormones on the membranes results in the averaging of their individual activating effects. When the hormones were added one after another, the extent of adenylate cyclase activation corresponded to that induced by the first hormone; the activation was insensitive to the effect of the second hormone added. It is assumed that the outer membrane of myocardium cells contains a adenylate cyclase and three types of receptors, each being capable to interact with the same form of enzyme. The activity of adenylate cyclase is determined by the type of the receptor, to which it is bound and by the amount of the enzyme-receptor complex. | [Isolation, purification and characterization of regulatory properties of adenylate cyclase from rabbit heart]. Rabbit heart membranes possessing the adenylate cyclase activity were isolated and purified by extraction with high ionic strength solutions and centrifugation in the sucrose density gradient. It was shown that the membranes are characterized by a high percentage of cholesterol (molar ratio cholesterol/phospholipids is 0.24) and an increased activity of Na, K-ATPase, which suggests the localization of adenylate cyclase in the sarcolemma. During centrifugation in the sucrose density gradient the activities of andenylate cyclase and Na,K-ATPase are not separated. Treatment of heart sarcolemma with a 0.3% solution of lubrol WX results in 10--20% solubilization of adenylate cyclase. Purification of the enzyme in the membrane fraction is accompanied by a decrease in the activity of phosphodiesterase; however, about 2% of the heart diesterase total activity cannot be removed from the sarcolemma even after its treatment with 0.3% lubrol WX. Epinephrine and NaF activate adenylate cyclase without changing the pH dependence of the enzyme. The alpha-adrenergic antagonist phentolamine has no effect on the adenylate cyclase activation by catecholamines, glucagon and histamine; the beta-adrenergic antagonist alprenolol competitively inhibits the effects of isoproterenol, epinephrine and norepinephrine, having no effect on the enzyme activation by glucagon and histamine. There is no competition between epinephrine, glucagon and histamine for the binding site of the hormone; however, there may occur a competition between the hormone receptors for the binding to the enzyme. A combined action of several hormones on the membranes results in the averaging of their individual activating effects. When the hormones were added one after another, the extent of adenylate cyclase activation corresponded to that induced by the first hormone; the activation was insensitive to the effect of the second hormone added. It is assumed that the outer membrane of myocardium cells contains a adenylate cyclase and three types of receptors, each being capable to interact with the same form of enzyme. The activity of adenylate cyclase is determined by the type of the receptor, to which it is bound and by the amount of the enzyme-receptor complex. | [
-0.06390316784381866,
-0.043323565274477005,
-0.03115001507103443,
0.04073401167988777,
0.03726132959127426,
-0.0026026784908026457,
0.034663669764995575,
0.02103532664477825,
-0.05661167576909065,
-0.041392982006073,
0.016044825315475464,
-0.07034126669168472,
-0.030464213341474533,
0.03519180044531822,
-0.008402793668210506,
-0.012000023387372494,
-0.0031487452797591686,
0.01542083453387022,
-0.053934600204229355,
-0.028790902346372604,
-0.0033791055902838707,
0.003119541797786951,
0.033841297030448914,
-0.018770430237054825,
0.007196751423180103,
0.04141463711857796,
-0.02499418705701828,
-0.05181538313627243,
-0.10215294361114502,
-0.17801788449287415,
0.015632962808012962,
0.011633890680968761,
0.0294375941157341,
-0.007962842471897602,
-0.00988586526364088,
0.02654322050511837,
-0.026677239686250687,
-0.010612658224999905,
-0.021790292114019394,
0.07336179167032242,
0.02385941706597805,
0.0031972425058484077,
0.023306673392653465,
0.08580832928419113,
0.04159527271986008,
-0.02458132430911064,
-0.028528008610010147,
-0.06909578293561935,
0.10972616821527481,
-0.021436519920825958,
0.04176871106028557,
0.03867528587579727,
-0.025466440245509148,
0.025005491450428963,
0.04727264493703842,
-0.02908642590045929,
-0.01122339814901352,
0.08624222874641418,
0.008912183344364166,
0.022099481895565987,
0.029521768912672997,
0.03971540555357933,
-0.1315533071756363,
0.07403282821178436,
0.016777122393250465,
-0.004641309380531311,
-0.008707013912498951,
-0.011690245009958744,
0.042054492980241776,
0.04567641019821167,
-0.0343351885676384,
0.04490312188863754,
-0.013818799518048763,
-0.02869662083685398,
-0.013369924388825893,
-0.003405967727303505,
0.0005113020888529718,
0.019504262134432793,
0.04690015688538551,
0.04372907057404518,
-0.015661466866731644,
0.008891582489013672,
0.0009615930030122399,
-0.016032174229621887,
-0.054056838154792786,
0.011999805457890034,
0.04285890609025955,
-0.07099843770265579,
-0.024027647450566292,
-0.017172375693917274,
-0.06810566782951355,
-0.07929209619760513,
-0.03385652229189873,
-0.05145830288529396,
-0.07264436781406403,
0.016706373542547226,
-0.0014131354400888085,
-0.017960982397198677,
0.01067856140434742,
0.3623969852924347,
0.02268526516854763,
0.017058156430721283,
-0.08042897284030914,
0.049291569739580154,
-0.009877405129373074,
0.014365349896252155,
-0.016001028940081596,
-0.01930380053818226,
-0.06325249373912811,
0.032545991241931915,
0.07945846021175385,
0.009891306050121784,
0.0030399495735764503,
-0.01798122748732567,
-0.0019419440068304539,
0.003757441183552146,
0.020419036969542503,
0.006489073392003775,
0.015453620813786983,
0.05366511270403862,
-0.0550580732524395,
-0.020166680216789246,
0.0061623393557965755,
-0.0024348630104213953,
0.036912113428115845,
0.013164793141186237,
-0.07727029919624329,
0.13527709245681763,
0.09443183243274689,
-0.014078493230044842,
0.07259184122085571,
0.06830853968858719,
-0.039378829300403595,
0.035967692732810974,
-0.010819901712238789,
-0.01080504059791565,
0.016475139185786247,
-0.05559879168868065,
0.048878107219934464,
-0.015127887018024921,
-0.013749788515269756,
-0.05752991512417793,
-0.011937876231968403,
-0.07228593528270721,
-0.0192245002835989,
0.08820648491382599,
0.05584332346916199,
0.05311072990298271,
-0.0567159540951252,
0.002335929311811924,
0.017244696617126465,
0.020277775824069977,
-0.053334470838308334,
0.09123486280441284,
-0.020794041454792023,
0.022227957844734192,
-0.0033723528031259775,
0.020572511479258537,
0.012613214552402496,
-0.02142288163304329,
-0.11509943753480911,
-0.04774153605103493,
0.008368550799787045,
0.08350397646427155,
0.021870112046599388,
0.010465818457305431,
-0.07030506432056427,
0.03975440561771393,
-0.02384064346551895,
0.015998274087905884,
0.009310144931077957,
0.031962066888809204,
-0.018299248069524765,
-0.0053792488761246204,
-0.04337325692176819,
0.00337108108215034,
0.03698873892426491,
0.01189423818141222,
0.07831798493862152,
-0.060247357934713364,
-0.0023863003589212894,
0.026873722672462463,
0.013011539354920387,
0.04162748157978058,
0.008709559217095375,
-0.019183533266186714,
-0.006802764721214771,
-0.02639508806169033,
0.03390297293663025,
0.015068217180669308,
0.009211676195263863,
0.004297164734452963,
-0.08365553617477417,
0.019770432263612747,
-0.06959336251020432,
-0.07789355516433716,
-0.03642965108156204,
0.0019071170827373862,
0.01794111542403698,
0.012837246060371399,
0.11716506630182266,
0.01984841749072075,
0.01335427537560463,
0.06019732356071472,
-0.011011612601578236,
0.0864124447107315,
-0.03332694619894028,
0.017852848395705223,
0.04810052365064621,
0.010881857946515083,
0.024219078943133354,
0.03463613614439964,
-0.04638976603746414,
-0.0006193998269736767,
-0.056664563715457916,
0.05208544060587883,
0.008171994239091873,
0.025607043877243996,
0.08187543600797653,
-0.049708180129528046,
0.024118145927786827,
-0.032228365540504456,
-0.015747912228107452,
-0.28646543622016907,
-0.014558209106326103,
-0.027351275086402893,
0.00445569958537817,
0.06398889422416687,
-0.0501413568854332,
0.024235833436250687,
-0.04724162071943283,
-0.03437173366546631,
-0.058912623673677444,
-0.009971635416150093,
0.05601901188492775,
-0.04068785160779953,
-0.01747947372496128,
0.004670716822147369,
0.04262612760066986,
-0.021347779780626297,
-0.00019459643226582557,
0.017974479123950005,
0.05859706178307533,
0.051254428923130035,
-0.009141495451331139,
0.004025166388601065,
0.02127302810549736,
0.10452966392040253,
-0.02645217254757881,
0.11980292946100235,
0.04012264311313629,
-0.004055359400808811,
0.0484982393682003,
-0.006905653513967991,
0.0211357269436121,
-0.03017958253622055,
-0.10182145982980728,
-0.01253876369446516,
-0.050376903265714645,
-0.007011630106717348,
-0.014826240949332714,
0.001471713767386973,
0.031433917582035065,
-0.015333001501858234,
0.04280148446559906,
-0.008108461275696754,
-0.030105914920568466,
-0.04642772302031517,
-0.034891095012426376,
-0.041654713451862335,
0.0242397952824831,
-0.02013041079044342,
-0.04067367687821388,
0.07563099265098572,
0.011733606457710266,
-0.011811608448624611,
-0.05179906636476517,
0.025657374411821365,
-0.011701526120305061,
-0.03480665385723114,
-0.002637250814586878,
-0.05116778984665871,
0.024550359696149826,
-0.009277288801968098,
0.010172766633331776,
-0.01548089925199747,
0.03543826565146446,
-0.03571860119700432,
0.034671422094106674,
-0.0006241751834750175,
-0.02177581936120987,
0.026103878393769264,
0.019711576402187347,
-0.014970962889492512,
0.06579847633838654,
0.0007457645260728896,
-0.050503361970186234,
0.08245276659727097,
0.0472441092133522,
-0.030489467084407806,
-0.01974959298968315,
-0.07397640496492386,
0.006467603147029877,
-0.0553886778652668,
-0.05122150480747223,
0.076575368642807,
-0.014829551801085472,
-0.03455863893032074,
-0.018243711441755295,
0.0341455414891243,
0.009661571122705936,
0.03892764076590538,
-0.010736631229519844,
-0.0008332197903655469,
0.01185718271881342,
-0.03279324993491173,
-0.0561906136572361,
0.026096858084201813,
0.0004160648095421493,
-0.27677837014198303,
0.02093597501516342,
0.0036191975232213736,
-0.05398445203900337,
-0.0715167447924614,
-0.011763912625610828,
0.054401613771915436,
-0.03804904595017433,
-0.024516014382243156,
0.050137732177972794,
0.025783400982618332,
0.029487088322639465,
0.07067330181598663,
0.0565607063472271,
-0.03869592398405075,
0.0437348373234272,
0.0360913947224617,
-0.032326728105545044,
0.039044465869665146,
-0.016915922984480858,
0.00839933194220066,
-0.02136896550655365,
0.12214529514312744,
0.02587873488664627,
-0.04227626696228981,
0.041313763707876205,
0.0049226791597902775,
-0.018682589754462242,
-0.02816241793334484,
0.034517981112003326,
-0.0224539153277874,
0.015263906680047512,
0.08701924234628677,
-0.024639112874865532,
0.03551496937870979,
0.027691101655364037,
-0.016973456367850304,
0.009017087519168854,
-0.0030173216946423054,
-0.04583807662129402,
0.01192594412714243,
0.013539833016693592,
-0.08301849663257599,
-0.06709764897823334,
0.055122293531894684,
-0.08019459992647171,
-0.0022301804274320602,
-0.03670074790716171,
-0.011164287105202675,
0.018025308847427368,
0.011804685927927494,
-0.018122799694538116,
0.03985488787293434,
0.009882793761789799,
-0.0028370425570756197,
-0.023920640349388123,
-0.07158844918012619,
0.0025373566895723343,
0.006953762378543615,
0.014640799723565578,
-0.0393877811729908,
-0.044936031103134155,
0.023319577798247337,
0.08145779371261597,
0.05989237502217293
] |
PMID:27250 | [Properties of DNA-methylase reaction in isolated nuclei of normal and regenerating rat liver]. | Evidence from comparative determination of DNA radioactivity methylation degree of acidic extraction and chlorophormic deproteination of the samples suggest that the former technique is a more efficient one. The properties of the DNA-methylase reaction in isolated rat liver nuclei were studied. The DNA-methylase activity is found to be considerably stable during incubation of the nuclei at 37 degrees C; a broad pH-optimum in the alkaline region is observed (pH 8.6--9.8); this activity is inhibited by Mn2+, nucleotides, actynomycin and S-adenosyl methionine analogs and is activated by Mg2+; the incorporation of methyl groups into DNA is reversible. The data suggest that the DNA-methylase activities of the nuclei isolated at different stages of regeneration do not show substantial variations. No differences in DNA methylation before and after DNA synthesis in the regenerating nuclei were observed. Inhibition of DNA synthesis in the course of regeneration does not decrease the level of DNA methylation. The interrelationship between methylation and replication of DNA is discussed. | [Properties of DNA-methylase reaction in isolated nuclei of normal and regenerating rat liver]. Evidence from comparative determination of DNA radioactivity methylation degree of acidic extraction and chlorophormic deproteination of the samples suggest that the former technique is a more efficient one. The properties of the DNA-methylase reaction in isolated rat liver nuclei were studied. The DNA-methylase activity is found to be considerably stable during incubation of the nuclei at 37 degrees C; a broad pH-optimum in the alkaline region is observed (pH 8.6--9.8); this activity is inhibited by Mn2+, nucleotides, actynomycin and S-adenosyl methionine analogs and is activated by Mg2+; the incorporation of methyl groups into DNA is reversible. The data suggest that the DNA-methylase activities of the nuclei isolated at different stages of regeneration do not show substantial variations. No differences in DNA methylation before and after DNA synthesis in the regenerating nuclei were observed. Inhibition of DNA synthesis in the course of regeneration does not decrease the level of DNA methylation. The interrelationship between methylation and replication of DNA is discussed. | [
-0.039079222828149796,
0.0017831966979429126,
-0.04934591427445412,
0.01714620366692543,
0.005181243643164635,
-0.01707977056503296,
0.036990560591220856,
0.03452514111995697,
-0.041438959538936615,
-0.009633834473788738,
0.03673388436436653,
-0.03400580212473869,
-0.013056972064077854,
-0.0060582710430026054,
-0.03088257648050785,
0.035204872488975525,
-0.004401281476020813,
-0.0022216003853827715,
-0.05887359380722046,
-0.05894545838236809,
-0.02479447051882744,
0.009586870670318604,
0.06678985059261322,
0.00858248956501484,
0.02097952924668789,
-0.00812484323978424,
-0.0011577645782381296,
-0.005643568933010101,
-0.03560766950249672,
-0.2016969472169876,
0.03323598578572273,
0.036225512623786926,
0.03940828889608383,
-0.04022695869207382,
0.019899237900972366,
-0.028582416474819183,
-0.014873136766254902,
0.004066867753863335,
-0.03745162859559059,
0.025316301733255386,
0.035342272371053696,
0.010247436352074146,
-0.0038211734499782324,
-0.0006409627385437489,
0.022612081840634346,
0.0900510922074318,
0.0004330982337705791,
-0.01747157610952854,
0.03832365944981575,
0.03807022050023079,
-0.036129169166088104,
0.022278359159827232,
-0.03731318563222885,
0.06799846887588501,
0.0014184871688485146,
0.028085093945264816,
0.053854554891586304,
0.07197029888629913,
0.01002860814332962,
0.01579153537750244,
0.017183270305395126,
0.013487945310771465,
-0.12057571113109589,
0.04650305211544037,
0.03426632657647133,
-0.010210475884377956,
-0.0024615281727164984,
-0.038125600665807724,
0.059798888862133026,
0.04283321276307106,
-0.0753244087100029,
0.054367709904909134,
0.010549106635153294,
0.04282338172197342,
0.03136487305164337,
-0.012407366186380386,
-0.01925886608660221,
-0.0005679114838130772,
0.042904358357191086,
0.03237682208418846,
-0.038205452263355255,
-0.024137789383530617,
0.01485214289277792,
-0.03136767819523811,
-0.02214975282549858,
0.021900756284594536,
-0.00933564268052578,
-0.0059783062897622585,
-0.018291311338543892,
0.04653050750494003,
-0.029727259650826454,
-0.00767629686743021,
-0.0030887031462043524,
-0.05209757760167122,
-0.07828971743583679,
0.010201194323599339,
0.03823385015130043,
0.009656863287091255,
0.053202010691165924,
0.3476755917072296,
0.0033803146798163652,
0.05308280885219574,
-0.07696579396724701,
-0.004994317889213562,
-0.07858525216579437,
-0.00972919724881649,
-0.0010622257832437754,
-0.0019586822018027306,
0.006727763917297125,
0.06274732202291489,
0.0984765887260437,
0.0907120555639267,
0.038963694125413895,
-0.026620829477906227,
0.026262981817126274,
0.0019643257837742567,
0.046007465571165085,
0.02824070304632187,
-0.04029854014515877,
0.001487401663325727,
-0.020837586373090744,
-0.03476539999246597,
0.04200219362974167,
-0.038447458297014236,
0.053713466972112656,
0.022605393081903458,
0.0530644915997982,
0.08495640754699707,
0.057456932961940765,
0.06604569405317307,
0.01179963257163763,
-0.04017648845911026,
0.017133567482233047,
0.004165859892964363,
-0.015558529645204544,
-0.008127818815410137,
0.006376489996910095,
-0.02395671047270298,
-0.011033175513148308,
-0.01308791246265173,
-0.0116804800927639,
-0.08476126939058304,
-0.056168440729379654,
-0.02732304111123085,
-0.0629596933722496,
0.03214527294039726,
-0.04252247512340546,
0.05023506283760071,
0.01454212050884962,
0.001548169064335525,
-0.024409975856542587,
0.06917885690927505,
-0.03339703008532524,
0.040133167058229446,
-0.03708207607269287,
-0.014476190321147442,
-0.04032055661082268,
-0.06662002205848694,
0.011531482450664043,
-0.009179693646728992,
-0.06472781300544739,
-0.010554250329732895,
-0.0378362201154232,
0.12820541858673096,
0.03412949666380882,
-0.01599607802927494,
-0.05823657289147377,
0.014753497205674648,
0.010298057459294796,
0.027258165180683136,
0.004023090470582247,
0.00482453778386116,
-0.017826158553361893,
0.0029759914614260197,
-0.04380272701382637,
-0.061517007648944855,
0.014341778121888638,
0.014252672903239727,
0.002092124428600073,
0.009083430282771587,
0.048049286007881165,
-0.06519608199596405,
-0.03799065202474594,
0.023772064596414566,
0.028041446581482887,
-0.01058891974389553,
-0.09291598945856094,
0.05352141335606575,
0.019219782203435898,
0.048299554735422134,
0.014797202311456203,
-0.003657621331512928,
-0.021584296599030495,
0.01300062332302332,
-0.03701702132821083,
-0.0716715008020401,
0.0019131357548758388,
0.06996339559555054,
-0.008446121588349342,
-0.03290156275033951,
0.07904721796512604,
-0.002081786748021841,
0.013137816451489925,
0.0045143235474824905,
-0.04689597338438034,
0.03723965212702751,
-0.027178537100553513,
0.008853585459291935,
0.06047923117876053,
0.01098496001213789,
-0.07110392302274704,
0.0046725147403776646,
-0.016322296112775803,
-0.010788564570248127,
-0.04200565069913864,
0.05042561888694763,
0.041589125990867615,
-0.01967945694923401,
0.042389821261167526,
0.02197725512087345,
-0.005198286846280098,
-0.10842934995889664,
-0.09664316475391388,
-0.27847999334335327,
0.002835487714037299,
-0.03400362655520439,
0.03879908099770546,
0.029972752556204796,
-0.04988790303468704,
0.03239782899618149,
-0.03200019523501396,
0.03721840679645538,
-0.04697609320282936,
0.018350202590227127,
0.027430765330791473,
-0.057049062103033066,
-0.06019821763038635,
-0.02016051858663559,
-0.012747452594339848,
0.029567290097475052,
0.010948636569082737,
0.022254392504692078,
0.01762869395315647,
0.040223486721515656,
-0.014510889537632465,
0.009393353015184402,
-0.051725808531045914,
0.062399040907621384,
-0.009519333019852638,
0.09341836720705032,
0.002106147352606058,
0.007582941558212042,
0.03286438435316086,
-0.015075994655489922,
0.03582143783569336,
0.013744859024882317,
-0.10545141249895096,
0.01294364407658577,
-0.08933892101049423,
-0.031354933977127075,
-0.006320044863969088,
0.008502142503857613,
0.042234040796756744,
0.002126086037606001,
-0.004065004643052816,
-0.040099382400512695,
-0.07694941014051437,
-0.032290879637002945,
-0.001086787204258144,
0.012427294626832008,
0.03286788985133171,
0.028387650847434998,
-0.06358388066291809,
0.018264655023813248,
0.03780240938067436,
-0.014527629129588604,
-0.06965137273073196,
-0.0018445701571181417,
-0.007451176177710295,
-0.05483603477478027,
0.02951440028846264,
-0.057689424604177475,
-0.04166937619447708,
0.00030999587033875287,
-0.005040209740400314,
-0.0006886663031764328,
0.05332614481449127,
-0.005992254242300987,
-0.011113550513982773,
0.021635351702570915,
0.02153676003217697,
0.018955666571855545,
-0.005200535524636507,
0.0640823096036911,
0.10651429742574692,
-0.026012809947133064,
-0.03317015618085861,
0.0656379759311676,
0.02974344789981842,
-0.10481998324394226,
-0.06867041438817978,
-0.00820603035390377,
0.037242792546749115,
0.01865108497440815,
-0.02131546474993229,
0.06956064701080322,
-0.015507202595472336,
-0.016434112563729286,
0.04249328747391701,
0.01194354984909296,
-0.010870254598557949,
0.009854047559201717,
0.026093250140547752,
0.07549598813056946,
-0.018031736835837364,
0.012574570253491402,
-0.03326154500246048,
0.06592809408903122,
-0.024999290704727173,
-0.2942391335964203,
-0.037613384425640106,
-0.04347798973321915,
0.017452554777264595,
-0.029662108048796654,
0.013538345694541931,
0.03829943388700485,
0.026591381058096886,
-0.07954414188861847,
0.06617654114961624,
0.019943445920944214,
0.015722129493951797,
0.07563531398773193,
0.08862718939781189,
-0.047001294791698456,
-0.00717192655429244,
0.02456199750304222,
0.01800992153584957,
-0.0028147425036877394,
-0.06875362247228622,
0.0037020756863057613,
-0.07710065692663193,
0.152448832988739,
-0.0370565764605999,
-0.031988419592380524,
0.037714023143053055,
0.0279755387455225,
0.05936757102608681,
0.0008599539869464934,
-0.008229857310652733,
0.01274228747934103,
0.053189702332019806,
0.07426659017801285,
0.0019765913020819426,
0.03284389153122902,
0.06300624459981918,
-0.050799913704395294,
-0.0006084563210606575,
-0.05045214295387268,
-0.0070368037559092045,
-0.04591752588748932,
0.019184397533535957,
-0.04488936811685562,
-0.002469766652211547,
0.02019411325454712,
-0.10508637130260468,
-0.024974895641207695,
-0.045864399522542953,
-0.0071303448639810085,
0.00513862818479538,
-0.03161226958036423,
0.015452629886567593,
0.011424587108194828,
-0.02450360171496868,
-0.014470655471086502,
-0.04891910403966904,
-0.03240005299448967,
-0.015758177265524864,
0.05181237682700157,
-0.02752230316400528,
-0.004365833476185799,
0.056614071130752563,
0.023325331509113312,
0.1606794148683548,
-0.011374639347195625
] |
PMID:27251 | [Effect of cortisol on the content of mRNA coding inducible tyrosine aminotransferase isoenzyme in rat liver]. | In vitro estimation of synthesis of inducible tyrosine aminotransferase isoenzyme, directed by poly-A-containing RNA from liver of intact and corticol treated rats, is carried out. Total poly-A-containing RNA from liver polyribosomes of intact and induced rats was translated in cell-free system from wheat germs. Two antibodies immunoprecipitation was used to identify the translocation product (tyrosine aminotransferase). It was found that a synthesis of a specific protein product, precipitated by antibodies to tyrosine aminotransferase, takes place in cell-free system under translation of polysomic poly-A-containing liver RNA. The amount of immunoprecipitated product indicates, that the content of individual poly-A-containing mRNA for inducible tyrosine aminotransferase isoenzyme in liver of cortisol-induced rats is considerably higher than in intact animals. | [Effect of cortisol on the content of mRNA coding inducible tyrosine aminotransferase isoenzyme in rat liver]. In vitro estimation of synthesis of inducible tyrosine aminotransferase isoenzyme, directed by poly-A-containing RNA from liver of intact and corticol treated rats, is carried out. Total poly-A-containing RNA from liver polyribosomes of intact and induced rats was translated in cell-free system from wheat germs. Two antibodies immunoprecipitation was used to identify the translocation product (tyrosine aminotransferase). It was found that a synthesis of a specific protein product, precipitated by antibodies to tyrosine aminotransferase, takes place in cell-free system under translation of polysomic poly-A-containing liver RNA. The amount of immunoprecipitated product indicates, that the content of individual poly-A-containing mRNA for inducible tyrosine aminotransferase isoenzyme in liver of cortisol-induced rats is considerably higher than in intact animals. | [
-0.030116168782114983,
0.02965199202299118,
-0.054430022835731506,
0.017272384837269783,
-0.015786316245794296,
-0.0004232134378980845,
0.10788191854953766,
-0.029636282473802567,
-0.0490594245493412,
0.018544476479291916,
0.02291042171418667,
-0.06526677310466766,
0.01999117061495781,
0.012862687930464745,
-0.03104400634765625,
0.052401114255189896,
0.03276517242193222,
-0.019013237208127975,
-0.05922167748212814,
-0.05103403329849243,
-0.005599800497293472,
-0.02750212326645851,
0.07777737081050873,
-0.014315428212285042,
0.04409230500459671,
0.019284935668110847,
0.006093394942581654,
-0.014238950796425343,
-0.03145286813378334,
-0.19773322343826294,
0.014092284254729748,
-0.03712047263979912,
0.015564667992293835,
-0.03333565220236778,
-0.0028744759038090706,
0.0015752512263134122,
-0.03140170872211456,
0.02338499389588833,
0.004362682346254587,
0.04153183475136757,
0.03251960128545761,
0.034674666821956635,
0.01785212568938732,
0.041467197239398956,
0.007924878969788551,
0.009083006531000137,
-0.03199578821659088,
-0.02754543535411358,
0.05419699102640152,
-0.02131558768451214,
-0.00849210750311613,
0.004352358635514975,
0.023650534451007843,
0.06625297665596008,
0.005227582063525915,
0.04411846771836281,
0.023367134854197502,
0.08846936374902725,
-0.0019408257212489843,
0.018862133845686913,
-0.021620843559503555,
0.01693801023066044,
-0.15620768070220947,
0.06404531002044678,
0.09032263606786728,
0.05049667879939079,
-0.02642171084880829,
-0.030118463560938835,
0.02912115678191185,
0.01938715949654579,
-0.06776158511638641,
0.04396089166402817,
0.005417999811470509,
0.02645017020404339,
0.01928100734949112,
0.00021821455447934568,
-0.039927251636981964,
-0.030511125922203064,
0.06579238921403885,
0.03153008595108986,
-0.005326752085238695,
0.04576703533530235,
0.023785514757037163,
0.002367849927395582,
-0.0372404046356678,
0.019254256039857864,
-0.039947934448719025,
-0.025189435109496117,
0.01779934950172901,
-0.0003183553635608405,
0.00454665208235383,
-0.027707546949386597,
0.02842997945845127,
0.03613300994038582,
-0.06928644329309464,
0.015696045011281967,
0.005504802335053682,
-0.05320396274328232,
0.022312264889478683,
0.3824247121810913,
-0.003161817556247115,
0.00205234088934958,
-0.07893231511116028,
-0.05828351154923439,
-0.0497782900929451,
-0.0005730260163545609,
0.0050694686360657215,
0.03117085061967373,
-0.03853920102119446,
0.06490879505872726,
0.01187924388796091,
0.02957397885620594,
0.03231433406472206,
-0.01371755450963974,
-0.043353378772735596,
-0.021803464740514755,
0.01853959634900093,
0.04494386538863182,
-0.04472354054450989,
0.043360814452171326,
-0.0011170505313202739,
0.014509513042867184,
-0.007357134949415922,
-0.040820248425006866,
0.04176045581698418,
-0.015933841466903687,
0.02275351621210575,
0.0932820588350296,
0.08477754890918732,
0.0036388130392879248,
0.041561711579561234,
0.06156750023365021,
-0.03134984150528908,
0.028509484604001045,
0.0430719256401062,
-0.006433641072362661,
0.006529031787067652,
-0.032454874366521835,
0.007816711440682411,
0.017425190657377243,
0.030770299956202507,
-0.0671013742685318,
-0.012628711760044098,
0.007974157109856606,
-0.04802214726805687,
0.06535936146974564,
-0.008726906962692738,
-0.02313579060137272,
0.03496298938989639,
-0.03259925916790962,
0.014945197850465775,
0.07303375750780106,
-0.038359854370355606,
0.05990780144929886,
-0.01503308117389679,
-0.019518427550792694,
-0.05391718074679375,
-0.05564601346850395,
-0.018267782405018806,
-0.044004831463098526,
-0.03613374009728432,
-0.04906671866774559,
-0.03557657450437546,
0.04595184698700905,
0.013488732278347015,
-0.02680763229727745,
-0.01780865527689457,
0.039184872061014175,
0.035064756870269775,
-0.05584704875946045,
0.03889276459813118,
-0.019713839516043663,
-0.032844532281160355,
0.02610187418758869,
-0.09013275057077408,
-0.07321489602327347,
0.0030239028856158257,
0.03494447469711304,
0.012143834494054317,
-0.05063501000404358,
0.030085692182183266,
-0.05436397343873978,
-0.01028776727616787,
0.061752524226903915,
-0.03552665561437607,
-0.011390086263418198,
-0.016172468662261963,
-0.020108940079808235,
0.0023445053957402706,
-0.001781612983904779,
0.017374800518155098,
0.059128060936927795,
-0.1313834935426712,
0.020848479121923447,
-0.06505488604307175,
-0.011704270727932453,
-0.023009348660707474,
0.11010130494832993,
0.030973998829722404,
-0.011062213219702244,
0.07265917211771011,
0.02752271108329296,
0.037459153681993484,
0.07779555767774582,
-0.023025695234537125,
-0.021783631294965744,
0.01992662064731121,
-0.0068145073018968105,
0.03069065511226654,
0.008013165555894375,
-0.028737427666783333,
-0.026534385979175568,
-0.023162968456745148,
0.041938379406929016,
-0.05657683685421944,
0.03799349069595337,
0.04794333130121231,
0.013351377099752426,
0.09425363689661026,
0.016781961545348167,
-0.014873120002448559,
-0.10142409056425095,
0.004358208738267422,
-0.31799596548080444,
0.003807659726589918,
0.034881897270679474,
-0.051897015422582626,
0.026530249044299126,
-0.04463610798120499,
0.048350028693675995,
-0.03951640427112579,
0.04368923231959343,
0.016139887273311615,
0.05361850932240486,
0.03193124756217003,
-0.01045636460185051,
-0.017088226974010468,
-0.017515134066343307,
0.07376967370510101,
0.026971986517310143,
-0.022589128464460373,
0.038614675402641296,
-0.0054408228024840355,
0.012813899666070938,
-0.051585208624601364,
0.025071939453482628,
-0.019182411953806877,
-0.005080488044768572,
-0.010124942287802696,
0.11928172409534454,
0.04363797605037689,
-0.02591541036963463,
0.034929804503917694,
-0.0176702793687582,
0.06154191866517067,
0.004450936336070299,
-0.1402072310447693,
0.0006604712107218802,
-0.04481116682291031,
-0.03388568386435509,
-0.005770915187895298,
0.01058768667280674,
0.023921241983771324,
-0.024825772270560265,
-0.021720711141824722,
-0.02893347293138504,
-0.05313664302229881,
-0.05587032437324524,
-0.030211985111236572,
0.017114857211709023,
-0.006629509851336479,
0.005126953590661287,
-0.0016056570457294583,
0.07316248118877411,
-0.02328033372759819,
0.03511420637369156,
-0.0032321454491466284,
0.019783971831202507,
-0.002915619406849146,
-0.0362265519797802,
-0.024061640724539757,
-0.06122851371765137,
0.01587965525686741,
0.01748722791671753,
0.004330668598413467,
-0.02417636290192604,
0.008819139562547207,
-0.028088996186852455,
0.01733354665338993,
0.007831144146621227,
0.010790635831654072,
0.01986118033528328,
0.05514048412442207,
0.011192054487764835,
0.0891387015581131,
0.004909414332360029,
0.0033025166485458612,
0.03751905634999275,
-0.010966184549033642,
-0.06831514835357666,
-0.039009612053632736,
-0.10646717250347137,
0.004139875527471304,
-0.018491031602025032,
-0.03417591750621796,
0.03889438509941101,
0.03576045483350754,
0.0048353769816458225,
0.01585353910923004,
0.049012862145900726,
-0.005812281277030706,
0.01671273447573185,
0.0017398251220583916,
0.024353407323360443,
-0.010173260234296322,
0.008826824836432934,
-0.027253292500972748,
0.05818518251180649,
-0.0005177870625630021,
-0.29486462473869324,
-0.022334638983011246,
0.00021855952218174934,
-0.01955227367579937,
0.008260786533355713,
0.021831771358847618,
-0.03586050868034363,
-0.04083870351314545,
-0.08547316491603851,
0.018519865348935127,
0.03552497178316116,
0.014472979120910168,
0.05266141518950462,
0.04579482972621918,
-0.03505339473485947,
0.03783848509192467,
0.04052632674574852,
-0.024966975674033165,
0.048726603388786316,
-0.04823722317814827,
-0.0004443683719728142,
-0.05074748769402504,
0.08839176595211029,
-0.026921292766928673,
-0.007167892064899206,
0.017748404294252396,
-0.011800194159150124,
0.03562956303358078,
0.008312692865729332,
0.05367344990372658,
-0.01408572681248188,
-0.0016081096837297082,
0.06158040463924408,
0.025700729340314865,
0.006133693270385265,
0.08177272230386734,
-0.06258121132850647,
0.03871532157063484,
0.027982447296380997,
-0.004442968405783176,
-0.06191951781511307,
0.013724290765821934,
-0.043016500771045685,
-0.050105731934309006,
0.03253266587853432,
-0.019864091649651527,
-0.08728480339050293,
-0.04100579395890236,
-0.01943146251142025,
0.004942450672388077,
-0.04457058012485504,
0.010093356482684612,
-0.01678544469177723,
0.02101803943514824,
0.005327045451849699,
0.015767043456435204,
-0.018638284876942635,
-0.05123866721987724,
-0.006407655775547028,
0.014049533754587173,
-0.015017880126833916,
-0.008469371125102043,
-0.046484965831041336,
0.10735931992530823,
0.004775735083967447
] |
PMID:27252 | Evaluating clustering methods for psychiatric diagnosis. | This report represents an empirical evaluation of the major clustering approaches on psychiatric diagnostic data. Experienced psychiatrists, using 17 psychopathological variables, developed 88 archetypal psychiatric patients to represent four diagnostic categories (manic-depressive depressed, manic-depressive manic, simple schizophrenic, and paranoid schizophrenic). Ten computerized methods representative of the major clustering approaches and using various measures of similarity between patients were applied to this data set to develop de novo patient groupings. Evaluative criteria included the concordance of clustering output to the structure of the original data, and clustering replicability. Considerable differences were obtained among clustering methods. The best-ranked procedures were nearest centroid sorting methods and complete and centroid linkage hierarchical methods. The overall poorest ranking were obtained for multivariate normal mixture analysis and facial representation of multidimensional points. Further evaluation of cluster analytic methods on real biological and psychosocial data sets yielded similar rankings. | Evaluating clustering methods for psychiatric diagnosis. This report represents an empirical evaluation of the major clustering approaches on psychiatric diagnostic data. Experienced psychiatrists, using 17 psychopathological variables, developed 88 archetypal psychiatric patients to represent four diagnostic categories (manic-depressive depressed, manic-depressive manic, simple schizophrenic, and paranoid schizophrenic). Ten computerized methods representative of the major clustering approaches and using various measures of similarity between patients were applied to this data set to develop de novo patient groupings. Evaluative criteria included the concordance of clustering output to the structure of the original data, and clustering replicability. Considerable differences were obtained among clustering methods. The best-ranked procedures were nearest centroid sorting methods and complete and centroid linkage hierarchical methods. The overall poorest ranking were obtained for multivariate normal mixture analysis and facial representation of multidimensional points. Further evaluation of cluster analytic methods on real biological and psychosocial data sets yielded similar rankings. | [
-0.017424549907445908,
-0.004596676677465439,
-0.00512488791719079,
0.01025701779872179,
0.039507947862148285,
0.026385800912976265,
0.038352832198143005,
0.024996263906359673,
0.027895145118236542,
-0.05926857888698578,
0.0774851068854332,
-0.045243941247463226,
0.02665848098695278,
-0.0006697926437482238,
-0.008330178447067738,
0.028764227405190468,
0.05207587778568268,
0.021068092435598373,
0.007024553604424,
-0.011348150670528412,
-0.04297369346022606,
-0.017275650054216385,
-0.006016888190060854,
-0.0024682190269231796,
0.010072285309433937,
0.06272220611572266,
-0.00350629398599267,
-0.0009896480478346348,
-0.0659431591629982,
-0.13582029938697815,
0.03576543927192688,
0.042508453130722046,
0.08640388399362564,
0.009046612307429314,
-0.008295739069581032,
0.006838625762611628,
0.021713774651288986,
0.08114530891180038,
-0.03465272858738899,
-0.027012508362531662,
0.022139351814985275,
0.012392308562994003,
-0.010899029672145844,
-0.010755536146461964,
-0.027704309672117233,
-0.003317458089441061,
-0.06527941673994064,
0.020259391516447067,
0.008794356137514114,
-0.024156002327799797,
-0.0352071076631546,
-0.030359474942088127,
-0.030141370370984077,
0.08756553381681442,
-0.021326610818505287,
0.0018680511275306344,
0.047143932431936264,
-0.017500320449471474,
-0.016474813222885132,
0.015162174589931965,
0.041555605828762054,
0.04135391116142273,
-0.10455510765314102,
-0.002291309181600809,
0.05536875128746033,
0.007847457192838192,
-0.025243476033210754,
-0.07764992117881775,
0.017233792692422867,
-0.003598390147089958,
-0.029824228957295418,
0.028483670204877853,
0.03285737708210945,
0.06107889115810394,
0.015784842893481255,
0.006779529619961977,
-0.014085693284869194,
-0.006297058891505003,
-0.01806464232504368,
-0.015510911121964455,
-0.028735341504216194,
0.010553560219705105,
0.03485943749547005,
-0.03908495604991913,
0.05170399695634842,
-0.009757373481988907,
-0.0018953123362734914,
-0.048307813704013824,
-0.016627000644803047,
-0.022250736132264137,
0.015669221058487892,
0.021622978150844574,
-0.023085109889507294,
-0.007021684665232897,
-0.05338282138109207,
-0.044452592730522156,
0.009276388213038445,
-0.025874024257063866,
0.007837483659386635,
0.3884744942188263,
-0.021471241489052773,
0.012312429957091808,
0.03990532457828522,
-0.04361994192004204,
-0.07055296003818512,
-0.05409802868962288,
-0.008087036199867725,
-0.10638375580310822,
0.014993659220635891,
0.01426736917346716,
0.012610248290002346,
-0.008831394836306572,
0.06553243845701218,
-0.08834122121334076,
-0.02190234884619713,
-0.02545062266290188,
0.035682667046785355,
0.01846870593726635,
0.06293244659900665,
-0.0025961031205952168,
-0.0025078444741666317,
-0.014416675083339214,
0.035726148635149,
-0.026430249214172363,
0.04481791332364082,
-0.02446606755256653,
-0.03335599973797798,
0.06392082571983337,
0.05399772524833679,
-0.04197282716631889,
0.03476067632436752,
0.07824680954217911,
-0.07463005185127258,
0.04757925495505333,
0.006876498926430941,
0.008346248418092728,
0.05359320342540741,
0.007635360583662987,
0.019709600135684013,
0.034048084169626236,
0.049243293702602386,
-0.051244914531707764,
0.03424610197544098,
-0.019482316449284554,
-0.06651873141527176,
0.1929301768541336,
-0.0746232345700264,
-0.016240041702985764,
-0.017149874940514565,
0.021873289719223976,
0.057766154408454895,
0.008658744394779205,
-0.08957390487194061,
-0.06118999794125557,
-0.02696850337088108,
0.020886540412902832,
0.035606276243925095,
-0.025032980367541313,
-0.029332835227251053,
0.03132927045226097,
-0.01425226591527462,
-0.0306691974401474,
-0.00958137959241867,
0.12692005932331085,
0.04953784868121147,
-0.04123111814260483,
-0.032821543514728546,
-0.02842576429247856,
0.013450408354401588,
-0.006808598525822163,
0.03772479668259621,
0.04627958685159683,
-0.005838459357619286,
0.052545368671417236,
-0.08018992841243744,
-0.057120852172374725,
-0.004895634483546019,
0.007672636304050684,
0.005336765665560961,
-0.015684697777032852,
0.03148726746439934,
0.020752176642417908,
-0.05750734731554985,
0.05002779886126518,
0.004679705016314983,
-0.006497033406049013,
-0.03274324908852577,
0.004432998597621918,
0.02461073361337185,
-0.008442840538918972,
-0.051261089742183685,
-0.054363034665584564,
-0.02874695509672165,
0.02224614843726158,
-0.039857205003499985,
-0.0722149908542633,
-0.015456663444638252,
-0.037049490958452225,
-0.0787140503525734,
0.034792523831129074,
0.044842321425676346,
0.04621826112270355,
0.00939467828720808,
0.019119082018733025,
0.009776989929378033,
0.030232131481170654,
0.013380789197981358,
-0.030662447214126587,
0.06292768567800522,
0.029707955196499825,
-0.02545076236128807,
-0.012530423700809479,
0.019221095368266106,
0.01400369219481945,
-0.022213157266378403,
0.023668436333537102,
0.05575944110751152,
0.05533196032047272,
0.03727224841713905,
0.0700487568974495,
-0.0020726339425891638,
0.0025524308439344168,
-0.07713733613491058,
-0.27451810240745544,
-0.05213417112827301,
0.00965311098843813,
0.0026965888682752848,
-0.033546332269907,
-0.028765877708792686,
0.014986887574195862,
-0.04655948653817177,
-0.0034353858791291714,
0.029209768399596214,
-0.026490436866879463,
0.019539762288331985,
-0.012219355441629887,
0.021603571251034737,
-0.031395744532346725,
-0.013743961229920387,
0.020408181473612785,
-0.01269435789436102,
-0.0932009220123291,
0.0032308115623891354,
0.005403703078627586,
0.01073985081166029,
0.056879643350839615,
-0.05062839016318321,
0.006876942701637745,
-0.009650174528360367,
0.12700486183166504,
-0.01982843317091465,
-0.03768082708120346,
0.04852266237139702,
-0.008196692913770676,
0.00851668044924736,
-0.024613482877612114,
-0.07107644528150558,
0.06745626032352448,
-0.006556999403983355,
0.0016316839028149843,
-0.06190876290202141,
-0.02075222320854664,
0.0342264324426651,
-0.06622619926929474,
-0.022922838106751442,
0.031724713742733,
-0.021876001730561256,
-0.09095391631126404,
0.002605868736281991,
0.036156341433525085,
0.0421125628054142,
-0.014783387072384357,
-0.016032524406909943,
-0.007861118763685226,
-0.02049528807401657,
0.039684705436229706,
-0.04143664985895157,
-0.024409307166934013,
-0.0295256357640028,
-0.10879415273666382,
-0.05223817750811577,
-0.044029515236616135,
-0.015066944062709808,
0.07734540104866028,
-0.057057932019233704,
0.02317636087536812,
-0.05223901942372322,
0.017704622820019722,
-0.03568238765001297,
-0.040792666375637054,
0.04714171588420868,
0.007813449949026108,
-0.018608523532748222,
-0.030830666422843933,
0.1343766450881958,
-0.03272518143057823,
0.008866050280630589,
0.0874725952744484,
0.01962827518582344,
-0.003226174507290125,
-0.0012085746275261045,
-0.01295093260705471,
-0.010811187326908112,
0.03970383107662201,
-0.03785603120923042,
0.0059920065104961395,
0.0656462088227272,
0.014002902433276176,
0.011420559138059616,
0.03223467245697975,
-0.00380678940564394,
0.01792917773127556,
-0.0068964650854468346,
0.023299269378185272,
-0.016932135447859764,
-0.04745569825172424,
-0.015865683555603027,
-0.011334091424942017,
0.05269203335046768,
-0.27894461154937744,
0.0325167179107666,
-0.03485428914427757,
0.05662450194358826,
0.0070896330289542675,
-0.005628359504044056,
-0.020132802426815033,
0.015102311968803406,
0.02938639000058174,
-0.055135179311037064,
0.05340742692351341,
0.03319951146841049,
0.1049359068274498,
-0.0506683811545372,
-0.04625749960541725,
0.029115019366145134,
0.022042442113161087,
-0.056718774139881134,
0.06085988134145737,
-0.035912323743104935,
0.010570294223725796,
0.012934223748743534,
0.17895767092704773,
-0.04697967320680618,
0.02628709189593792,
0.0298243947327137,
0.02581295743584633,
-0.025380779057741165,
-0.04369216412305832,
-0.019438108429312706,
0.0003267631691414863,
-0.0032884133979678154,
0.07678911089897156,
-0.0007259983103722334,
0.0298438910394907,
-0.0008942010463215411,
-0.007485582027584314,
-0.025483783334493637,
0.054475087672472,
-0.02716960199177265,
0.03145705536007881,
0.011385713703930378,
-0.006847793702036142,
-0.013589069247245789,
0.09333763271570206,
0.01717832125723362,
0.007128364406526089,
-0.07351743429899216,
-0.03315849229693413,
0.01530179288238287,
-0.031201088801026344,
0.026969898492097855,
-0.010640802793204784,
-0.02261229045689106,
0.03843093290925026,
0.05392780527472496,
0.0059586986899375916,
0.04045141115784645,
-0.020341232419013977,
-0.09679444879293442,
-0.05015125125646591,
0.04209357127547264,
0.002779829315841198,
0.06321968138217926,
0.012666678987443447
] |
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.