id
stringlengths 7
10
| title
stringlengths 8
354
| content
stringlengths 91
4.1k
| contents
stringlengths 164
4.24k
| embedding
listlengths 384
384
|
|---|---|---|---|---|
PMID:8201 | Critical opening pressure and reactivity of tail vessels in conscious hypertensive rats. | The possible role of increased vascular reactivity in the mechanism of experimental hypertension was studied by measurements of the critical opening pressure (COP) of tail vessels in conscious rats. In hypertension induced by administration of desoxycorticosterone acetate (DOCA) and replacement of the drinking water by 1% NaCl solution (DOCA-NaCl hypertension), and in one-kidney Goldblatt renovascular hypertension, the raised level of blood pressure was associated with an increased COP of the tail vessels when measured both before and after ganglionic blockade. In rats treated with either DOCA alone or 1% NaCl alone there was no significant increase in systolic blood pressure (SBP) or COP relative to the corresponding controls. In all four experimental series intravenous infusion of angiotensin or norepinephrine in conscious ganglion-blocked rats produced dose-dependent increases in SBP and COP. In DOCA-NaCl hypertensive rats but not in renovascular hypertensives, nor in rats treated with DOCA alone or 1% NaCl alone, the increase in COP for a given increment in dose of angiotensin or norepinephrine was significantly greater than in the control rats. It is concluded that in DOCA-NaCl hypertension there is a true increase in the reactivity of the smooth muscle of the resistance vessels to angiotensin and norepinephrine. In renovascular hypertension this is not the case and other factors must therefore be involved in causing the increased blood pressure and COP. | Critical opening pressure and reactivity of tail vessels in conscious hypertensive rats. The possible role of increased vascular reactivity in the mechanism of experimental hypertension was studied by measurements of the critical opening pressure (COP) of tail vessels in conscious rats. In hypertension induced by administration of desoxycorticosterone acetate (DOCA) and replacement of the drinking water by 1% NaCl solution (DOCA-NaCl hypertension), and in one-kidney Goldblatt renovascular hypertension, the raised level of blood pressure was associated with an increased COP of the tail vessels when measured both before and after ganglionic blockade. In rats treated with either DOCA alone or 1% NaCl alone there was no significant increase in systolic blood pressure (SBP) or COP relative to the corresponding controls. In all four experimental series intravenous infusion of angiotensin or norepinephrine in conscious ganglion-blocked rats produced dose-dependent increases in SBP and COP. In DOCA-NaCl hypertensive rats but not in renovascular hypertensives, nor in rats treated with DOCA alone or 1% NaCl alone, the increase in COP for a given increment in dose of angiotensin or norepinephrine was significantly greater than in the control rats. It is concluded that in DOCA-NaCl hypertension there is a true increase in the reactivity of the smooth muscle of the resistance vessels to angiotensin and norepinephrine. In renovascular hypertension this is not the case and other factors must therefore be involved in causing the increased blood pressure and COP. | [
-0.06232776120305061,
0.011742760427296162,
0.027388721704483032,
0.0006749346503056586,
0.000197737492271699,
-0.013443278148770332,
0.07621420174837112,
0.022821571677923203,
-0.04579978436231613,
-0.05126882344484329,
0.012940737418830395,
-0.06091638281941414,
-0.04103190451860428,
0.011873281560838223,
-0.03238414600491524,
-0.004230813588947058,
0.027441762387752533,
0.005483114160597324,
-0.08255045861005783,
0.021944977343082428,
-0.013198859058320522,
0.0002752765140030533,
0.06079547479748726,
-0.029097024351358414,
0.040131229907274246,
-0.004269990138709545,
0.0040086619555950165,
-0.031412187963724136,
-0.023702561855316162,
-0.2038288563489914,
-0.018163256347179413,
-0.012606421485543251,
-0.013995549641549587,
-0.03930968418717384,
0.01384970173239708,
0.0032421601936221123,
-0.008024662733078003,
0.038696106523275375,
-0.004708489403128624,
0.060260411351919174,
0.01879957504570484,
0.027125870808959007,
-0.003969563636928797,
0.03056950867176056,
-0.015777379274368286,
0.0303700752556324,
-0.014434447512030602,
0.03285583108663559,
0.08249200880527496,
0.015301701612770557,
0.03312596306204796,
0.012649727053940296,
-0.018353041261434555,
0.04864083603024483,
0.03044116497039795,
0.005562080070376396,
0.0404178649187088,
-0.008568456396460533,
0.005376338493078947,
-0.017181552946567535,
0.07775186002254486,
0.07542228698730469,
-0.16514088213443756,
0.0721651017665863,
0.061456747353076935,
-0.011061418801546097,
-0.07015619426965714,
0.05126511678099632,
0.006965407636016607,
0.03532642871141434,
0.004532492719590664,
0.06832059472799301,
0.004378720186650753,
-0.015569320879876614,
0.015712635591626167,
-0.05165761709213257,
-0.03589008376002312,
-0.025689508765935898,
0.05303916707634926,
0.015253637917339802,
0.02736600860953331,
0.018103063106536865,
-0.020372161641716957,
-0.03284718841314316,
0.00802409928292036,
-0.07836325466632843,
0.0014578906120732427,
-0.05320223420858383,
-0.044100433588027954,
0.01585323177278042,
-0.06913727521896362,
-0.022207830101251602,
-0.0189975555986166,
0.010392320342361927,
-0.03871234506368637,
0.03641749173402786,
-0.000341355218552053,
-0.03260641545057297,
-0.02782699652016163,
0.37997594475746155,
-0.016992880031466484,
0.006564801093190908,
-0.05203922092914581,
0.015476361848413944,
0.028770294040441513,
0.021224245429039,
-0.01280678529292345,
0.012827490456402302,
0.004329259041696787,
0.01882237382233143,
0.05785057321190834,
-0.00288801989518106,
0.036400631070137024,
-0.03697095811367035,
-0.052355557680130005,
-0.04753582179546356,
0.016450539231300354,
-0.013112715445458889,
0.0015078203286975622,
-0.00402634683996439,
0.013081591576337814,
-0.005336491856724024,
-0.01181822083890438,
-0.022794175893068314,
-0.00006064622357371263,
-0.00448278384283185,
0.04431883245706558,
0.027934718877077103,
0.06719912588596344,
-0.016191286966204643,
0.02836471050977707,
-0.0032982544507831335,
-0.07684788107872009,
0.0009670971194282174,
0.052731748670339584,
-0.05649234354496002,
0.0664985403418541,
-0.015835082158446312,
-0.056087676435709,
0.013786585070192814,
-0.06504788249731064,
0.005983402021229267,
-0.0019903434440493584,
-0.07171837240457535,
-0.031497806310653687,
0.09567475318908691,
0.05492477864027023,
-0.0020681999158114195,
-0.057743385434150696,
-0.022597376257181168,
-0.007507627364248037,
0.010806424543261528,
-0.03175272047519684,
0.032904744148254395,
-0.01712021231651306,
0.02454296499490738,
-0.06965769827365875,
0.03243858739733696,
0.03264003247022629,
-0.015484591014683247,
-0.04396308213472366,
-0.04256189987063408,
-0.019748076796531677,
0.012676870450377464,
0.061815906316041946,
0.014551818370819092,
0.0013148573925718665,
0.014775319956243038,
0.017384737730026245,
0.030057469382882118,
0.004672475624829531,
0.06018047034740448,
0.0031145811080932617,
-0.04813195392489433,
-0.009275502525269985,
-0.012313407845795155,
0.028777077794075012,
-0.040560532361269,
0.020424064248800278,
-0.003416509833186865,
0.019876541569828987,
0.011633996851742268,
-0.014932876452803612,
0.03844528645277023,
-0.005198182072490454,
0.00786333903670311,
-0.09035220742225647,
-0.009565325453877449,
-0.006972880102694035,
0.07398299127817154,
-0.011633646674454212,
-0.004745673853904009,
-0.06907512992620468,
-0.023487448692321777,
-0.010300803929567337,
-0.05884424224495888,
0.008262759074568748,
0.033242836594581604,
-0.031453922390937805,
0.023160342127084732,
0.03928180783987045,
0.03016630746424198,
-0.004013487137854099,
0.009955160319805145,
0.02159707248210907,
0.08160668611526489,
-0.018144305795431137,
0.01975853741168976,
0.052534643560647964,
-0.016506800428032875,
-0.010213492438197136,
0.0935370922088623,
0.02522849105298519,
-0.009885801933705807,
-0.04006485641002655,
-0.00021425796148832887,
0.022046012803912163,
0.0076840464025735855,
0.055651724338531494,
0.0704776793718338,
0.09051898121833801,
-0.06626646220684052,
0.024141842499375343,
-0.32842564582824707,
-0.04872078076004982,
-0.027548914775252342,
-0.02813752368092537,
0.05223402753472328,
-0.024856431409716606,
0.02493724226951599,
-0.049385059624910355,
-0.000011842756066471338,
-0.02769090235233307,
0.006282292306423187,
0.05280597507953644,
-0.03904340788722038,
-0.012533831410109997,
0.020558753982186317,
0.0790104866027832,
0.06349797546863556,
-0.0119441207498312,
0.020072733983397484,
0.01788567565381527,
0.02360561303794384,
0.019681774079799652,
-0.060516685247421265,
0.06125154718756676,
0.02556528151035309,
-0.00653955340385437,
0.10763072967529297,
-0.01715983636677265,
-0.02046106569468975,
-0.02662273310124874,
-0.0013812693068757653,
0.045837193727493286,
-0.03595181927084923,
-0.14114011824131012,
0.03532955422997475,
0.007937917485833168,
-0.026519203558564186,
0.004578698892146349,
-0.007659268565475941,
0.032891955226659775,
-0.00830420944839716,
0.04401969537138939,
-0.017795702442526817,
0.020875602960586548,
-0.05793385952711105,
-0.024121349677443504,
0.021559594199061394,
0.10220976918935776,
-0.037056826055049896,
-0.0021483944728970528,
0.015628008171916008,
-0.022018421441316605,
-0.057093385607004166,
-0.00008119456470012665,
0.050190865993499756,
-0.06160120666027069,
-0.054334551095962524,
0.024314094334840775,
-0.025675354525446892,
-0.05966433510184288,
0.00718146376311779,
0.03488263487815857,
0.026052914559841156,
-0.034853942692279816,
-0.02826748788356781,
0.06939137727022171,
0.035101018846035004,
0.005544672254472971,
0.05652569234371185,
0.0438467301428318,
-0.015404964797198772,
0.1192839965224266,
0.03612130507826805,
0.04932054877281189,
0.055928971618413925,
0.0023554149083793163,
-0.04476158320903778,
-0.03936178237199783,
-0.05645649880170822,
0.021708831191062927,
0.0011459244415163994,
-0.07288745045661926,
0.0798056572675705,
0.006539982743561268,
-0.053803373128175735,
-0.008259017951786518,
0.04167824983596802,
0.009624078869819641,
0.06967125833034515,
0.002803670708090067,
-0.008591325022280216,
-0.0013089198619127274,
-0.03728640452027321,
-0.028225703164935112,
0.07462651282548904,
0.03927437588572502,
-0.29928988218307495,
-0.03010476566851139,
-0.000318405480356887,
-0.029107557609677315,
-0.02791626565158367,
0.04656865447759628,
0.015591763891279697,
-0.01275542564690113,
-0.03536968305706978,
-0.020435074344277382,
-0.045618440955877304,
0.0475633479654789,
0.025854675099253654,
0.10027150809764862,
-0.05306842550635338,
-0.00985406432300806,
0.024973616003990173,
0.01701691560447216,
0.042655523866415024,
-0.04711966589093208,
-0.04234398528933525,
-0.0044641196727752686,
0.1253291517496109,
-0.0005679012974724174,
-0.0267641544342041,
0.0010750446235761046,
-0.02095162868499756,
-0.02456446923315525,
-0.02198978327214718,
-0.029730742797255516,
0.006984880194067955,
-0.0023654408287256956,
0.02143193408846855,
-0.013973139226436615,
0.04073334112763405,
0.0338948518037796,
-0.021030912175774574,
0.030241122469305992,
-0.004735996481031179,
-0.0326862707734108,
-0.008794385008513927,
0.015229444019496441,
-0.075289785861969,
-0.029884587973356247,
0.043031901121139526,
-0.040203437209129333,
-0.011920501478016376,
-0.020423218607902527,
0.03249972686171532,
-0.004541153088212013,
-0.02405892312526703,
-0.026051461696624756,
0.03146005794405937,
0.024093862622976303,
0.0066266730427742004,
-0.07625465840101242,
-0.016359146684408188,
-0.029943080618977547,
0.036912377923727036,
-0.060130152851343155,
-0.03182743117213249,
-0.008446941152215004,
-0.03436571732163429,
0.0958765372633934,
0.009675027802586555
] |
PMID:8202 | Effects of isoproterenol on cyclic-AMP metabolism in rat ventral prostate. | Beta-Adrenergic stimulation of the ventral prostate cyclic-AMP system was investigated by examining the influence of isoproterenol on endogenous cyclic-AMP levels as well as on the activities of adenylate cyclase CEC 4.6.1.1) and cyclic-AMP-dependent and independent protein kinases (EC 2.7.1.37). Administration of isoproterenol (1 mg/kg, ip) resulted in rapid elevation of adenylate cyclase activity (119%) and cyclic-AMP levels (593%). The observed isoproterenol-stimulated changes in cyclic-AMP metabolism of the ventral prostate were time-dependent and maximal stimulation was seen 5 min after treatment with this beta-adrenergic agonist. The increases in prostatic adenylate cyclase and cyclic-AMP also were related to the dose of isoproterenol administered and maximal enhancement of these parameters was seen with 1 mg/kg dose of the agonist. Whereas pretreatment of rats with propranolol (3mg/kg, ip) partially reversed these alterations, administration of an alpha-adrenergic antagonist, phentolamine, even at a dose of 5 mg/kg, failed to elicit any appreciable effect. Stimulation of prostatic soluble protein kinase by isoproterenol was associated with a decrease (33%) in the activity of the cyclic-AMP-dependent protein kinase with a concomitant increase (25%) in that of the independent enzyme. Whereas the ability of the enzyme to bind cyclic-(3H) AMP in vitro was decreased (54%) following isoproterenol treatment, the protein kinase activity ratio (-cyclic-AMP/+cyclic AMP) was significantly elevated from 0.51+/0.05 to 0.95+/0.08. Although propranolol alone had little or no effect on these parameters, it inhibited partially the isoproterenol-induced alterations in cyclic-AMP-dependent protein kinase and the cyclic-AMP binding capacity. Treatment with propranolol also blocked the increases in the kinase activity ratio and in the activity of cyclic-AMP-independent enzyme seen with isoproterenol. Data suggest that the concentration of ventral prostate cyclic-AMP as well as the activities of adenylate cyclase and cyclic-AMP-dependent and independent form of protein kinases are subject to modulation by beta-adrenergic stimulation. | Effects of isoproterenol on cyclic-AMP metabolism in rat ventral prostate. Beta-Adrenergic stimulation of the ventral prostate cyclic-AMP system was investigated by examining the influence of isoproterenol on endogenous cyclic-AMP levels as well as on the activities of adenylate cyclase CEC 4.6.1.1) and cyclic-AMP-dependent and independent protein kinases (EC 2.7.1.37). Administration of isoproterenol (1 mg/kg, ip) resulted in rapid elevation of adenylate cyclase activity (119%) and cyclic-AMP levels (593%). The observed isoproterenol-stimulated changes in cyclic-AMP metabolism of the ventral prostate were time-dependent and maximal stimulation was seen 5 min after treatment with this beta-adrenergic agonist. The increases in prostatic adenylate cyclase and cyclic-AMP also were related to the dose of isoproterenol administered and maximal enhancement of these parameters was seen with 1 mg/kg dose of the agonist. Whereas pretreatment of rats with propranolol (3mg/kg, ip) partially reversed these alterations, administration of an alpha-adrenergic antagonist, phentolamine, even at a dose of 5 mg/kg, failed to elicit any appreciable effect. Stimulation of prostatic soluble protein kinase by isoproterenol was associated with a decrease (33%) in the activity of the cyclic-AMP-dependent protein kinase with a concomitant increase (25%) in that of the independent enzyme. Whereas the ability of the enzyme to bind cyclic-(3H) AMP in vitro was decreased (54%) following isoproterenol treatment, the protein kinase activity ratio (-cyclic-AMP/+cyclic AMP) was significantly elevated from 0.51+/0.05 to 0.95+/0.08. Although propranolol alone had little or no effect on these parameters, it inhibited partially the isoproterenol-induced alterations in cyclic-AMP-dependent protein kinase and the cyclic-AMP binding capacity. Treatment with propranolol also blocked the increases in the kinase activity ratio and in the activity of cyclic-AMP-independent enzyme seen with isoproterenol. Data suggest that the concentration of ventral prostate cyclic-AMP as well as the activities of adenylate cyclase and cyclic-AMP-dependent and independent form of protein kinases are subject to modulation by beta-adrenergic stimulation. | [
-0.046410705894231796,
-0.02801712043583393,
-0.024553976953029633,
0.008760749362409115,
0.024446170777082443,
0.008543698117136955,
-0.007726862095296383,
0.03515114635229111,
-0.059125833213329315,
-0.02542959526181221,
0.08586699515581131,
-0.04656791687011719,
-0.004496575333178043,
0.04705917835235596,
-0.02892749384045601,
0.01952183246612549,
0.035676758736371994,
0.003443059278652072,
-0.011674754321575165,
-0.042030807584524155,
0.010613693855702877,
-0.03056802973151207,
-0.006319474428892136,
-0.004518270958214998,
-0.01027910877019167,
0.020766688510775566,
-0.012945335358381271,
0.01110132783651352,
-0.09836502373218536,
-0.20057140290737152,
0.027986301109194756,
0.026572072878479958,
-0.004618392791599035,
-0.019222192466259003,
-0.027517840266227722,
-0.006098915357142687,
-0.008749188855290413,
0.02675008773803711,
-0.041024867445230484,
0.07361839711666107,
0.026680415496230125,
0.014482840895652771,
0.003121795132756233,
0.010750781744718552,
0.06851974874734879,
0.009543688967823982,
-0.06437249481678009,
-0.052271418273448944,
0.12270458787679672,
0.0038834663573652506,
0.041056420654058456,
-0.007424569688737392,
0.02316834032535553,
0.0313386507332325,
0.004159632604569197,
-0.030687689781188965,
0.02812272682785988,
0.09208868443965912,
-0.025291036814451218,
0.03702959418296814,
-0.02877665124833584,
0.051381614059209824,
-0.16167910397052765,
0.059111643582582474,
0.03364628925919533,
0.041764892637729645,
-0.04458323493599892,
-0.017064949497580528,
0.04548108950257301,
0.06890473514795303,
-0.03605418652296066,
0.029059384018182755,
0.026011142879724503,
-0.020173659548163414,
0.0003431668446864933,
-0.019647149369120598,
-0.009876674041152,
0.018542924895882607,
0.009400651790201664,
0.002543803071603179,
0.021960346028208733,
0.02021707408130169,
-0.04649009555578232,
-0.011892814189195633,
-0.013546702452003956,
-0.04846539720892906,
0.013122660107910633,
-0.040844619274139404,
-0.0056800697930157185,
-0.008534618653357029,
-0.08721829950809479,
-0.037228506058454514,
0.01814788579940796,
-0.05437230318784714,
-0.03367386758327484,
0.04367862641811371,
-0.018950292840600014,
-0.038561537861824036,
0.023798801004886627,
0.34399446845054626,
0.014422324486076832,
0.02321004681289196,
-0.05352954566478729,
-0.000579945626668632,
0.023258566856384277,
-0.03898308426141739,
0.010098992846906185,
0.03894438222050667,
-0.05862674489617348,
0.0781857892870903,
0.0655732974410057,
0.020370032638311386,
0.030746888369321823,
-0.06084425747394562,
-0.02647477574646473,
-0.04198531433939934,
0.040183212608098984,
0.004304016008973122,
-0.03388456627726555,
-0.018950579687952995,
-0.024025065824389458,
0.0013539076317101717,
0.014796617440879345,
0.03558371961116791,
0.05750143527984619,
0.008060961030423641,
-0.0016839983873069286,
0.08435991406440735,
0.0767553374171257,
0.01749200001358986,
0.08187758177518845,
0.07279165089130402,
-0.015552595257759094,
0.06956404447555542,
-0.00832639541476965,
-0.05783778801560402,
0.02079004794359207,
-0.024307692423462868,
0.03810197487473488,
0.050451964139938354,
-0.003765238681808114,
-0.04780091717839241,
-0.011988663114607334,
-0.04403681308031082,
-0.012665304355323315,
0.09284509718418121,
0.03844863921403885,
-0.019618384540081024,
-0.036108266562223434,
0.01592576876282692,
-0.014854337088763714,
0.027160517871379852,
-0.031033214181661606,
0.05537714064121246,
-0.04765037074685097,
0.044457823038101196,
-0.00812392309308052,
-0.025627627968788147,
-0.01841423660516739,
-0.042376160621643066,
-0.12310802191495895,
-0.03406905755400658,
0.00959023181349039,
0.06357643008232117,
0.036108095198869705,
0.0039831772446632385,
-0.06961190700531006,
0.0029067720752209425,
-0.0453430712223053,
0.0008692715782672167,
-0.010434342548251152,
-0.011613643728196621,
-0.018989507108926773,
-0.028788158670067787,
-0.005372917279601097,
0.0076063512824475765,
0.058654237538576126,
0.036293357610702515,
0.005087382160127163,
-0.034365031868219376,
0.0005334827001206577,
-0.030050823464989662,
-0.024301789700984955,
0.04326755553483963,
0.001036130590364337,
0.03734076768159866,
-0.06955006718635559,
-0.017423175275325775,
0.010737423785030842,
0.05510362237691879,
0.008496837690472603,
0.024936186149716377,
-0.12565355002880096,
0.026386266574263573,
-0.06368205696344376,
-0.03710423782467842,
-0.05798567458987236,
0.013016506098210812,
-0.03888426721096039,
0.004881273023784161,
0.09630028158426285,
0.004942933097481728,
0.027241379022598267,
0.04669027402997017,
-0.02498652972280979,
0.10048885643482208,
-0.04957061633467674,
0.049568597227334976,
0.09241684526205063,
0.013986238278448582,
-0.027800528332591057,
0.00967650581151247,
0.04167298972606659,
0.00922403298318386,
-0.042062535881996155,
0.08339338004589081,
-0.027242697775363922,
0.04362492635846138,
0.07373975962400436,
0.016028016805648804,
0.0147541593760252,
0.01065314095467329,
0.027523349970579147,
-0.2968807518482208,
-0.028186438605189323,
-0.022027350962162018,
-0.001942533883266151,
0.0240467619150877,
-0.042270366102457047,
0.027511481195688248,
-0.052414678037166595,
-0.002524307928979397,
-0.06550362706184387,
0.04004031419754028,
0.07434864342212677,
-0.04574307054281235,
0.0066966162994503975,
-0.03992825746536255,
0.08021648228168488,
0.011305691674351692,
-0.06549270451068878,
-0.00640703784301877,
-0.020286137238144875,
0.005970429629087448,
-0.02128661796450615,
-0.016746068373322487,
0.01779773458838463,
0.032755300402641296,
-0.009145409800112247,
0.09184174239635468,
0.0033232434652745724,
-0.01858753152191639,
0.04251024127006531,
-0.005123204085975885,
0.014545532874763012,
-0.019770033657550812,
-0.1436413675546646,
0.0049803960137069225,
-0.0592057928442955,
0.04889068379998207,
-0.021238522604107857,
-0.0006286916905082762,
0.016841739416122437,
-0.058287929743528366,
0.043928131461143494,
-0.014461888000369072,
-0.02388552762567997,
-0.05052245035767555,
0.025227712467312813,
0.02100955881178379,
0.04725675284862518,
-0.014410434290766716,
0.012784863822162151,
0.0016619650414213538,
0.008194836787879467,
-0.01869533210992813,
-0.0606982596218586,
0.029504157602787018,
-0.02931569516658783,
-0.04776526242494583,
-0.010475577786564827,
-0.036803144961595535,
0.007725485134869814,
0.021783415228128433,
0.019400829449295998,
0.006054925266653299,
0.02063409425318241,
-0.003966234624385834,
0.003018347080796957,
-0.017432840541005135,
-0.025578148663043976,
0.02485973760485649,
0.062093865126371384,
0.016846193000674248,
0.08143570274114609,
-0.026407955214381218,
-0.014733166433870792,
0.051871176809072495,
-0.002562340348958969,
-0.053525160998106,
-0.05787850543856621,
-0.04446365684270859,
-0.0014678084990009665,
-0.0345655232667923,
-0.06802081316709518,
0.0732971578836441,
0.03430292010307312,
-0.05754807963967323,
-0.025549722835421562,
-0.0051983497105538845,
0.02251952514052391,
0.05997309461236,
0.00918714888393879,
0.04456218332052231,
0.0037020184099674225,
-0.018898338079452515,
-0.032824501395225525,
0.04830082505941391,
0.030271176248788834,
-0.28595396876335144,
-0.0204936433583498,
0.02142709121108055,
-0.026280414313077927,
0.008392130956053734,
0.015484854578971863,
0.018117986619472504,
-0.05406451225280762,
-0.031158268451690674,
0.030456343665719032,
0.027802297845482826,
0.06589585542678833,
0.06846731156110764,
0.0593680664896965,
-0.009322762489318848,
0.05145781487226486,
0.03016500361263752,
-0.0041559599339962006,
0.01587984338402748,
-0.019910665228962898,
-0.01000312902033329,
-0.022528670728206635,
0.14261573553085327,
-0.012707220390439034,
-0.010862932540476322,
0.04488205537199974,
0.0022457612212747335,
-0.0019296782556921244,
-0.011196581646800041,
0.04216432198882103,
-0.10883644223213196,
0.022085044533014297,
0.06410706788301468,
-0.021402115002274513,
0.04336041212081909,
0.03205333277583122,
-0.008645167574286461,
0.0003105968935415149,
-0.016006343066692352,
-0.03558699041604996,
-0.0021301403176039457,
0.012905173934996128,
-0.07293842732906342,
-0.01786399818956852,
0.048713333904743195,
-0.12361413240432739,
-0.04558217525482178,
-0.015151827596127987,
0.016739575192332268,
0.012201632373034954,
-0.03132065013051033,
-0.014581957831978798,
0.022263243794441223,
0.06779135763645172,
-0.025606516748666763,
-0.013748164288699627,
-0.05550532415509224,
0.0017064952990040183,
-0.0016330685466527939,
-0.00402600783854723,
-0.026369430124759674,
-0.010729550383985043,
-0.010284650139510632,
0.08347637951374054,
0.04267570748925209
] |
PMID:8203 | Epinephrine-induced hepatic potassium movements before and after adrenergic blockade. | The epinephrine-induced loss and subsequent uptake of K+ by the liver was studied by measuring hepatic arterio-venous K+ differences and splanchnic blood flows in anesthetized dogs with chronically implanted portal vein catheters and celiac and superior mesenteric artery flow probes. When epinephrine was administered intraportally, neither alpha- nor beta-adrenergic blockade, singly or in combination, had significant effects upon the hyperkalemic or the hypokalemic phases in either hepatic venous or systemic arterial blood. It was concluded that the movements of K+ into and out of the liver caused by epinephrine are not mediated by the classical adrenergic receptors as defined by inhibition by specific blocking agents. | Epinephrine-induced hepatic potassium movements before and after adrenergic blockade. The epinephrine-induced loss and subsequent uptake of K+ by the liver was studied by measuring hepatic arterio-venous K+ differences and splanchnic blood flows in anesthetized dogs with chronically implanted portal vein catheters and celiac and superior mesenteric artery flow probes. When epinephrine was administered intraportally, neither alpha- nor beta-adrenergic blockade, singly or in combination, had significant effects upon the hyperkalemic or the hypokalemic phases in either hepatic venous or systemic arterial blood. It was concluded that the movements of K+ into and out of the liver caused by epinephrine are not mediated by the classical adrenergic receptors as defined by inhibition by specific blocking agents. | [
-0.0464077927172184,
-0.05327162519097328,
0.06604420393705368,
0.06721890717744827,
-0.019411660730838776,
-0.05462180823087692,
0.08470303565263748,
0.021699296310544014,
-0.04859489947557449,
0.007823936641216278,
-0.0015590423718094826,
-0.050374433398246765,
-0.025125684216618538,
0.02968243509531021,
-0.06506209075450897,
0.0012198662152513862,
0.006355620920658112,
-0.014917084947228432,
-0.08976074308156967,
-0.028377389535307884,
-0.027806146070361137,
0.0025298402179032564,
0.03777752444148064,
-0.006323688197880983,
-0.0030988482758402824,
0.009960934519767761,
0.013952737674117088,
-0.059131164103746414,
-0.02739875204861164,
-0.18022677302360535,
-0.02214636094868183,
-0.025287572294473648,
0.022730281576514244,
-0.009067902341485023,
-0.025647668167948723,
0.008831172250211239,
-0.052293479442596436,
0.04635458067059517,
-0.012570568360388279,
0.05962561070919037,
0.010571031831204891,
0.011139837093651295,
-0.027883872389793396,
0.057173363864421844,
0.00782568845897913,
0.015634974464774132,
-0.007084444165229797,
-0.027337215840816498,
0.11152687668800354,
0.025255979970097542,
0.02557271160185337,
0.007921138778328896,
0.055219877511262894,
0.05031699314713478,
0.043244846165180206,
-0.012550518848001957,
-0.004842639435082674,
0.0750591978430748,
0.020170941948890686,
0.03916934132575989,
-0.028522813692688942,
0.048179153352975845,
-0.15293915569782257,
0.07591942697763443,
0.05395572632551193,
-0.01461032498627901,
-0.04515475407242775,
0.015597843565046787,
0.02588552236557007,
0.04515237361192703,
0.003165608737617731,
0.022803520783782005,
-0.044019561260938644,
-0.0016880948096513748,
-0.01598622463643551,
-0.03526487946510315,
0.018097782507538795,
-0.00014485286374110729,
0.02241354249417782,
0.026408890262246132,
0.029236776754260063,
0.00577168446034193,
0.007880414836108685,
-0.04526164010167122,
0.011891252361238003,
-0.0350647047162056,
-0.00584499491378665,
-0.02694856747984886,
-0.02816404588520527,
-0.03416208177804947,
-0.03166966885328293,
0.0140595193952322,
-0.01735577918589115,
-0.004451148211956024,
-0.035972222685813904,
-0.025578796863555908,
-0.0526818223297596,
-0.005178655497729778,
-0.0073784030973911285,
0.38214799761772156,
0.009120997972786427,
0.06489327549934387,
-0.07656783610582352,
0.05481046810746193,
0.011938005685806274,
-0.03127918764948845,
-0.01215002778917551,
-0.040449704974889755,
-0.01178756169974804,
0.024827565997838974,
0.06106533482670784,
-0.021524367853999138,
0.05575308948755264,
-0.03768862038850784,
-0.034955330193042755,
0.004703231621533632,
0.05340644344687462,
0.04803171753883362,
0.003927141893655062,
0.06113096699118614,
-0.019339805468916893,
-0.006974535528570414,
-0.02017018012702465,
-0.014685065485537052,
0.03346184641122818,
-0.013075185008347034,
-0.040968213230371475,
0.07251748442649841,
0.021758709102869034,
0.023237092420458794,
0.06987525522708893,
0.040930576622486115,
-0.006055872887372971,
-0.010692068375647068,
-0.030721668154001236,
0.01291328389197588,
0.048648446798324585,
-0.04058939963579178,
0.026630038395524025,
-0.014926721341907978,
-0.04227770119905472,
-0.07647411525249481,
-0.015092550776898861,
-0.0986783504486084,
-0.06174759194254875,
0.0766737088561058,
0.08186931163072586,
-0.03981699049472809,
-0.021524429321289062,
0.015560947358608246,
-0.029390407726168633,
-0.03224353864789009,
0.006722783204168081,
0.058161601424217224,
-0.07500752806663513,
0.01358390599489212,
-0.029128925874829292,
0.035958874970674515,
-0.003517999080941081,
-0.029094712808728218,
-0.05746287852525711,
-0.09259665012359619,
-0.047007184475660324,
0.04992865025997162,
0.01938807964324951,
0.04607745632529259,
-0.00981198251247406,
-0.027978727594017982,
-0.00015730029554106295,
0.014274021610617638,
0.00414405157789588,
0.02354525588452816,
-0.04068174585700035,
-0.031968362629413605,
-0.025118496268987656,
0.000039879276300780475,
0.04462888464331627,
0.014494750648736954,
0.054334092885255814,
-0.10288053005933762,
0.05972542241215706,
0.03479612618684769,
-0.0013218135572969913,
0.030803345143795013,
0.05863627791404724,
-0.027002226561307907,
-0.05639930069446564,
0.023658432066440582,
-0.003961621318012476,
0.06211842596530914,
-0.033446285873651505,
0.012677722610533237,
-0.04880852252244949,
-0.011712122708559036,
-0.03385431319475174,
-0.006142195779830217,
-0.015752341598272324,
0.04960531368851662,
-0.018865957856178284,
0.0024247656110674143,
0.031559333205223083,
0.016915814951062202,
-0.012026375159621239,
0.09253013879060745,
-0.01449049822986126,
0.023961948230862617,
-0.016576766967773438,
-0.0024857602547854185,
0.011956635862588882,
-0.00575375696644187,
-0.012222309596836567,
0.05383210629224777,
0.06996265798807144,
-0.029548896476626396,
-0.0480138398706913,
-0.005953154526650906,
-0.07524045556783676,
0.04303184524178505,
0.08256494998931885,
-0.004385823383927345,
0.034592561423778534,
-0.04257605969905853,
0.06263453513383865,
-0.30792543292045593,
-0.015768179669976234,
0.02375042624771595,
0.054147426038980484,
0.015207525342702866,
-0.06895013153553009,
0.026711180806159973,
-0.038815539330244064,
-0.0038193061482161283,
-0.024303458631038666,
0.0043227896094322205,
0.04750463366508484,
-0.035379715263843536,
-0.025005163624882698,
0.018493298441171646,
0.0472838394343853,
0.021858587861061096,
-0.006126703694462776,
0.01278184074908495,
0.058135416358709335,
0.03854278475046158,
-0.0012248923303559422,
-0.025115497410297394,
-0.020919131115078926,
0.0034803212620317936,
0.00741428742185235,
0.11157043278217316,
0.016352294012904167,
-0.012812563218176365,
-0.0539093092083931,
-0.007269361522048712,
0.06184839457273483,
0.011579426936805248,
-0.08031561970710754,
0.006831607781350613,
-0.07238826155662537,
0.014595204964280128,
-0.0020930261816829443,
0.02598855458199978,
0.0030821494292467833,
-0.12072782218456268,
-0.006481177173554897,
-0.022837184369564056,
0.0008308736141771078,
0.0028116493485867977,
-0.01267538033425808,
0.003963280003517866,
0.028816914185881615,
-0.034224968403577805,
-0.008229785598814487,
0.02951117232441902,
0.017826857045292854,
-0.004875804763287306,
-0.0372183583676815,
0.0445837527513504,
-0.0263743307441473,
-0.04068439081311226,
-0.01634223945438862,
-0.01625254563987255,
-0.005340995267033577,
0.02274608425796032,
0.054031070321798325,
0.019303737208247185,
-0.013421259820461273,
0.015750644728541374,
0.05352288857102394,
0.05866692215204239,
0.0390351265668869,
0.049699801951646805,
0.03693519905209541,
0.016480891034007072,
0.15485602617263794,
-0.029818639159202576,
0.02068575844168663,
0.08372502774000168,
0.043146565556526184,
0.002806638367474079,
0.03171992674469948,
-0.02923823520541191,
0.03174252808094025,
0.007213194388896227,
-0.06383030116558075,
0.03951207548379898,
0.0014337386237457395,
-0.021097607910633087,
0.04278944805264473,
-0.012182563543319702,
-0.02727118693292141,
0.009466425515711308,
-0.044320445507764816,
-0.0718589648604393,
0.04473573714494705,
-0.031302522867918015,
-0.03262162208557129,
0.03659267723560333,
0.06845458596944809,
-0.25316616892814636,
0.03179636597633362,
0.04583503678441048,
-0.06669054180383682,
-0.05410699546337128,
0.05022558569908142,
-0.018131066113710403,
-0.0720256045460701,
-0.09946724772453308,
-0.02423970028758049,
-0.03246985375881195,
-0.004786522593349218,
0.08728959411382675,
0.03713091090321541,
-0.07261452823877335,
0.021092621609568596,
0.04887561500072479,
0.006184295751154423,
-0.04008185863494873,
-0.03309326618909836,
0.006648254115134478,
-0.021984191611409187,
0.12924954295158386,
-0.024319477379322052,
-0.05091419816017151,
-0.009719123132526875,
0.026075197383761406,
0.059072770178318024,
-0.042438384145498276,
-0.004571940284222364,
-0.020086124539375305,
0.013034728355705738,
0.05784888565540314,
-0.015908420085906982,
0.04953297972679138,
0.0469278059899807,
-0.03292078152298927,
0.005403714254498482,
-0.052709292620420456,
0.0039741890504956245,
-0.018579721450805664,
0.05375603586435318,
-0.02100670337677002,
-0.0660271942615509,
0.0298040509223938,
0.019772745668888092,
-0.04622385650873184,
-0.06995319575071335,
0.04596490040421486,
-0.030238203704357147,
-0.008876614272594452,
-0.03181874752044678,
0.014762327075004578,
0.06637924164533615,
0.007903308607637882,
-0.0026879378128796816,
-0.0793452262878418,
-0.011398409493267536,
0.010449713096022606,
-0.030534127727150917,
0.002795759355649352,
0.007658896967768669,
-0.01469836663454771,
0.054570239037275314,
0.03736526891589165
] |
PMID:8204 | Blockade of histamine-induced contractions of guinea pig ielum by beta-haloalkylamines. | In the longitudinal muscle strip of guinea pig ileum phenoxybenzamine (POB) produces a maximum parallel shift of 0.7 log units in the dose-response curve to histamine. In the presence of sodium thiosulfate in the wash fluid the parallel shift whith retention of maximum response increases to about 2 log units, and a similar value is obtained for Nethyl-N-(2-bromoethyl)-1-naphthylamine. The The agent N-ethyl-N- (2-chloroethyl)benzylamine produces a significantly smaller shift of dose-response curve of 1.53 log units before the maximum response becomes depressed. The receptor-specific depression of maximum response produced by higher doses of POB is reversed by sodium thiosulfate and by bovine serum albumin, while the parallel shift in dose-response curve is unaffected by both treatments. These findings may be explained by a hypothesis involving interaction of 2-haloalkylamines at two sites. | Blockade of histamine-induced contractions of guinea pig ielum by beta-haloalkylamines. In the longitudinal muscle strip of guinea pig ileum phenoxybenzamine (POB) produces a maximum parallel shift of 0.7 log units in the dose-response curve to histamine. In the presence of sodium thiosulfate in the wash fluid the parallel shift whith retention of maximum response increases to about 2 log units, and a similar value is obtained for Nethyl-N-(2-bromoethyl)-1-naphthylamine. The The agent N-ethyl-N- (2-chloroethyl)benzylamine produces a significantly smaller shift of dose-response curve of 1.53 log units before the maximum response becomes depressed. The receptor-specific depression of maximum response produced by higher doses of POB is reversed by sodium thiosulfate and by bovine serum albumin, while the parallel shift in dose-response curve is unaffected by both treatments. These findings may be explained by a hypothesis involving interaction of 2-haloalkylamines at two sites. | [
-0.0895061269402504,
0.026138857007026672,
0.0008437128271907568,
0.06039528548717499,
-0.005399841815233231,
0.009587215259671211,
0.04764064773917198,
0.007052481174468994,
-0.03925558552145958,
0.0025371687952429056,
0.009747247211635113,
-0.08710706979036331,
-0.03707098960876465,
0.02851882204413414,
0.012636895291507244,
0.006213588174432516,
0.001062246155925095,
-0.031061410903930664,
-0.06657195091247559,
-0.003796412842348218,
-0.026334870606660843,
0.005397158209234476,
0.009816423989832401,
-0.015224012546241283,
0.02153545431792736,
-0.03059259057044983,
-0.029600676149129868,
0.024900466203689575,
-0.02046283148229122,
-0.251103013753891,
-0.013831069692969322,
0.01360322441905737,
0.024191919714212418,
-0.048216089606285095,
0.015492087230086327,
0.040926121175289154,
-0.041558120399713516,
-0.013246569782495499,
0.013572504743933678,
0.03337981924414635,
0.030388379469513893,
0.055444493889808655,
0.02412787824869156,
0.020932238548994064,
0.0005876587238162756,
0.012006924487650394,
-0.0320899523794651,
-0.018660709261894226,
0.07824445515871048,
-0.01666341722011566,
0.04088784381747246,
-0.042964302003383636,
-0.005922394338995218,
0.050904665142297745,
0.04136423021554947,
0.005863697733730078,
-0.039055708795785904,
0.09481368958950043,
-0.035667784512043,
0.057369496673345566,
0.015695897862315178,
0.02273055538535118,
-0.15681789815425873,
0.08187371492385864,
0.06754986196756363,
-0.003007967956364155,
-0.027894550934433937,
-0.03718884289264679,
0.014271194115281105,
0.06346601992845535,
-0.054881397634744644,
0.011009301990270615,
0.004749844782054424,
-0.014940261840820312,
0.010189392603933811,
0.0012175652664154768,
0.012496785260736942,
0.04079725593328476,
-0.009068735875189304,
-0.03254713863134384,
-0.01701633259654045,
0.02745707891881466,
-0.031491030007600784,
-0.08804687857627869,
0.0036464922595769167,
-0.01656479947268963,
0.028078604489564896,
-0.018160976469516754,
-0.012382582761347294,
-0.005522118415683508,
-0.010351298376917839,
-0.026295635849237442,
0.0015869323397055268,
-0.036276768893003464,
-0.025044769048690796,
0.02297152206301689,
-0.0022530187852680683,
-0.025580434128642082,
0.02520471066236496,
0.34504204988479614,
0.0024201953783631325,
0.006401582155376673,
-0.10247718542814255,
-0.0006238305359147489,
0.022148557007312775,
-0.002397012198343873,
0.025356341153383255,
-0.0022307238541543484,
-0.01852298527956009,
0.08366018533706665,
0.08127685636281967,
0.005023333244025707,
0.02557332068681717,
-0.04859711974859238,
-0.05115365982055664,
0.01927293837070465,
0.01848561316728592,
0.028507297858595848,
-0.02077811025083065,
-0.012113314121961594,
0.011498610489070415,
0.008917409926652908,
0.014848913997411728,
-0.011622354388237,
0.03569569066166878,
0.03246635943651199,
-0.044592488557100296,
0.06451931595802307,
0.03172514587640762,
-0.01817270927131176,
0.04434641823172569,
0.010304955765604973,
0.011889258399605751,
0.00659120362251997,
-0.012061226181685925,
0.0016326085897162557,
0.05149291083216667,
-0.014954055659472942,
0.015495073050260544,
0.05002467334270477,
-0.0035476761404424906,
-0.07948990166187286,
-0.047181759029626846,
-0.09170985221862793,
-0.09534217417240143,
0.12242435663938522,
0.02023826166987419,
-0.01481638103723526,
-0.040747448801994324,
-0.008013841696083546,
-0.022571314126253128,
0.036666326224803925,
-0.011832042597234249,
-0.00014439833466894925,
-0.03176199272274971,
0.033022452145814896,
-0.023711541667580605,
0.040377601981163025,
0.014727943576872349,
0.0255239550024271,
-0.09338191151618958,
0.0005850191810168326,
0.015835672616958618,
0.07351423799991608,
-0.01394058857113123,
0.007932948879897594,
-0.025211019441485405,
0.019216330721974373,
0.021561795845627785,
0.013332135044038296,
0.006259252782911062,
0.0668065994977951,
-0.006126349791884422,
-0.020450767129659653,
-0.05709012970328331,
-0.017459051683545113,
0.05232980102300644,
0.023173660039901733,
0.0557926706969738,
-0.017881663516163826,
0.0020987396128475666,
0.0013942255172878504,
0.030990900471806526,
-0.016575489193201065,
0.06355249881744385,
0.04710835963487625,
-0.03788682818412781,
0.0018137990264222026,
0.03729024901986122,
0.005369151942431927,
0.019907884299755096,
0.013923797756433487,
-0.05411997064948082,
0.00911505427211523,
-0.0324736163020134,
-0.0660962164402008,
-0.07497302442789078,
0.042933542281389236,
-0.0031044173520058393,
-0.04235343635082245,
0.1289188265800476,
0.050393812358379364,
-0.039100710302591324,
0.07715564966201782,
0.025702057406306267,
0.030788671225309372,
0.029960427433252335,
-0.04437235742807388,
0.08912573009729385,
-0.0006181165226735175,
-0.004339734558016062,
0.0198321845382452,
0.023020533844828606,
-0.015800081193447113,
-0.07464858144521713,
0.027999188750982285,
0.02636786364018917,
0.0596737265586853,
0.054521579295396805,
0.014806174673140049,
0.01088886521756649,
-0.023731594905257225,
-0.016034584492444992,
-0.3131277561187744,
-0.028506148606538773,
0.00023382149811368436,
-0.031103283166885376,
0.06048749387264252,
-0.0455373153090477,
-0.0058824121952056885,
-0.08230971544981003,
0.01353993359953165,
-0.054896868765354156,
0.06481516361236572,
0.09072673320770264,
-0.05023637041449547,
-0.1252908855676651,
0.028079399839043617,
0.06461990624666214,
-0.006731410510838032,
0.042270176112651825,
0.016578370705246925,
0.0409260094165802,
0.02988603338599205,
-0.033091187477111816,
-0.041372641921043396,
0.027285240590572357,
0.0381181538105011,
-0.005418930668383837,
0.12727323174476624,
-0.0152128329500556,
-0.023827247321605682,
-0.01708049885928631,
0.018260398879647255,
0.04569894075393677,
0.0008224392659030855,
-0.02498598024249077,
-0.03613653779029846,
-0.02553534135222435,
-0.03763403370976448,
-0.018808575347065926,
-0.004273544531315565,
-0.020130785182118416,
-0.01495679747313261,
-0.005000303499400616,
0.022940881550312042,
-0.1076611801981926,
-0.0312623493373394,
-0.02495766244828701,
0.002033329103142023,
-0.02189096435904503,
-0.016241930425167084,
-0.03892606124281883,
0.01884530857205391,
-0.015526766888797283,
-0.00845860131084919,
-0.0007863549399189651,
0.03471292182803154,
-0.05839311331510544,
-0.04116210713982582,
-0.0075060282833874226,
-0.03780076280236244,
-0.018160320818424225,
0.0028510899282991886,
0.024646788835525513,
0.053735893219709396,
0.01922426000237465,
-0.04068835452198982,
0.012241816148161888,
0.05186831206083298,
-0.08376782387495041,
-0.005329504609107971,
-0.02299063466489315,
0.0265000332146883,
0.1315150260925293,
-0.04374777525663376,
0.03917352855205536,
0.05221038684248924,
0.033648423850536346,
0.020047197118401527,
-0.021641483530402184,
-0.13443678617477417,
0.002592799486592412,
-0.009129379875957966,
-0.03699318692088127,
0.03845894709229469,
0.021233951672911644,
-0.018055737018585205,
-0.024662867188453674,
0.05880783498287201,
-0.02151426114141941,
0.04198302701115608,
0.011423214338719845,
0.011674193665385246,
0.05095387622714043,
0.004839581903070211,
-0.031898993998765945,
0.010621455498039722,
0.01975276693701744,
-0.2612507939338684,
0.005636152345687151,
-0.028339697048068047,
0.022159701213240623,
-0.03654532507061958,
0.023721560835838318,
0.052283015102148056,
-0.023994283750653267,
-0.05237451568245888,
-0.022710297256708145,
-0.004572849255055189,
0.023122278973460197,
0.02783377282321453,
0.06709142029285431,
-0.0098534245043993,
-0.024455640465021133,
0.039335157722234726,
-0.004372331313788891,
0.024006087332963943,
-0.046330776065588,
-0.016013989225029945,
-0.03456078842282295,
0.12900693714618683,
0.05662733316421509,
0.00409125117585063,
0.049372173845767975,
-0.01072249747812748,
0.02521834522485733,
-0.008260229602456093,
-0.005763877648860216,
-0.03463253006339073,
0.022834058851003647,
0.08310459554195404,
-0.029360434040427208,
0.023100726306438446,
0.028514482080936432,
-0.07422176748514175,
-0.04907909035682678,
-0.004618239589035511,
0.013784398324787617,
-0.027729423716664314,
0.04278728738427162,
-0.041938990354537964,
-0.04887307062745094,
0.11456811428070068,
-0.0205974280834198,
-0.02649853378534317,
-0.059519633650779724,
0.021410426124930382,
0.013575511053204536,
-0.027011269703507423,
-0.02743596024811268,
0.01915254257619381,
0.04847419261932373,
0.019629422575235367,
-0.011970693245530128,
-0.021967872977256775,
-0.04715385660529137,
0.009208514355123043,
0.017447425052523613,
-0.0002164567558793351,
-0.01075038406997919,
-0.03930377960205078,
0.009503071196377277,
0.04768424853682518
] |
PMID:8205 | Postsplenectomy sepsis due to influenzal viremia and pneumococcemia. | A 31-year-old man, who had undergone splenectomy 18 months previously because of hereditary spherocytosis, suddenly became ill, with fever, vomiting, epigastric pain and shock, and died 10 hours after the onset of his symptoms. Autopsy showed influenzal viremia, pneumococcemia and bilateral adrenal hemorrhage. The rapid course of the patient's illness emphasizes the serious risk of sepsis for individuals who have had a splenectomy. Anti-influenza immunization in such patients should be considered. | Postsplenectomy sepsis due to influenzal viremia and pneumococcemia. A 31-year-old man, who had undergone splenectomy 18 months previously because of hereditary spherocytosis, suddenly became ill, with fever, vomiting, epigastric pain and shock, and died 10 hours after the onset of his symptoms. Autopsy showed influenzal viremia, pneumococcemia and bilateral adrenal hemorrhage. The rapid course of the patient's illness emphasizes the serious risk of sepsis for individuals who have had a splenectomy. Anti-influenza immunization in such patients should be considered. | [
-0.03779223561286926,
0.039744846522808075,
-0.0399298332631588,
-0.01647477224469185,
0.04758782312273979,
-0.023088181391358376,
0.08080517500638962,
0.06309019029140472,
-0.02398058772087097,
0.01194789819419384,
-0.00047576200449839234,
-0.0032555079087615013,
-0.00878012366592884,
-0.005913695320487022,
-0.05297662317752838,
-0.006621121428906918,
0.007029031403362751,
0.038485269993543625,
-0.13981416821479797,
0.04781095311045647,
-0.06807593256235123,
0.05429626256227493,
0.009689351543784142,
-0.018579499796032906,
0.031265437602996826,
-0.015209121629595757,
-0.014176495373249054,
-0.0006937279831618071,
-0.02147161401808262,
-0.1549484133720398,
-0.028398986905813217,
-0.06387264281511307,
-0.03143813833594322,
-0.04576379805803299,
0.015127040445804596,
-0.035587262362241745,
-0.019670633599162102,
0.004020363558083773,
-0.003904649056494236,
0.04252544417977333,
0.004704609978944063,
0.02890927903354168,
-0.006130534689873457,
0.027628114446997643,
0.0217494647949934,
0.017559191212058067,
0.01642143912613392,
-0.021580055356025696,
0.1744629442691803,
0.032300692051649094,
-0.009738488122820854,
0.002307729097083211,
0.009503714740276337,
0.08238807320594788,
0.0142580671235919,
-0.054634589701890945,
-0.02596493624150753,
0.002637837314978242,
-0.017793167382478714,
-0.008247483521699905,
-0.01342291571199894,
-0.0361299104988575,
-0.13148042559623718,
0.020047739148139954,
0.0674225464463234,
-0.007540334481745958,
0.012185071595013142,
-0.0804855152964592,
0.04565581679344177,
0.010289473459124565,
-0.029711682349443436,
0.013608992099761963,
-0.027978578582406044,
0.059286754578351974,
-0.05314655601978302,
0.020287320017814636,
-0.0047136335633695126,
-0.0859178751707077,
0.0337807834148407,
0.05708353593945503,
0.05104382336139679,
0.017753779888153076,
-0.007885942235589027,
-0.012498309835791588,
-0.0497368648648262,
-0.06024492532014847,
0.02012541890144348,
-0.05554081127047539,
-0.05230578035116196,
-0.026073789224028587,
0.04838787391781807,
-0.0026908772997558117,
-0.01414257287979126,
0.040055420249700546,
-0.0917033776640892,
-0.030040791258215904,
-0.044265929609537125,
0.04058777540922165,
0.0011630157241597772,
0.39286717772483826,
0.006234306376427412,
-0.011742813512682915,
-0.016772059723734856,
0.0033716135658323765,
0.004193493165075779,
-0.005195567850023508,
0.028587378561496735,
-0.018014054745435715,
-0.004862932488322258,
0.00027569744270294905,
-0.003154589096084237,
0.02002660743892193,
0.04583432525396347,
0.009784289635717869,
0.0027574619743973017,
0.03918074816465378,
0.07872750610113144,
0.05718996003270149,
-0.028190962970256805,
0.034359753131866455,
0.033122844994068146,
-0.005875403992831707,
0.050486646592617035,
-0.04084441438317299,
0.025853630155324936,
0.06304974108934402,
0.06723938882350922,
0.11002647876739502,
-0.016996903344988823,
0.0021999701857566833,
0.12187910079956055,
0.04011651128530502,
0.011862330138683319,
0.006784216966480017,
-0.005626751575618982,
-0.03247842937707901,
0.044581614434719086,
-0.025356298312544823,
0.06348344683647156,
-0.07312201708555222,
-0.004663930274546146,
-0.08717767894268036,
-0.029889630153775215,
-0.01682877168059349,
-0.05749191343784332,
0.02942512370646,
-0.021660424768924713,
0.02891109138727188,
-0.0617363378405571,
0.013964435085654259,
-0.035964228212833405,
0.04964426904916763,
-0.0059790522791445255,
0.017940517514944077,
0.030607257038354874,
0.019879385828971863,
-0.019970029592514038,
-0.014552468433976173,
0.04149184003472328,
0.0242405254393816,
-0.07958376407623291,
-0.061909087002277374,
-0.03193357214331627,
0.053824376314878464,
0.012842479161918163,
0.026382965967059135,
-0.002838282147422433,
-0.03955772519111633,
0.0013128023128956556,
0.04494323953986168,
0.008116756565868855,
-0.006280363071709871,
-0.021992117166519165,
-0.07467775046825409,
-0.004273650702089071,
0.05298207700252533,
0.015436979942023754,
-0.03259378671646118,
0.021554598584771156,
0.020888110622763634,
-0.008462133817374706,
0.0051924618892371655,
0.055182140320539474,
0.043940041214227676,
-0.0022848034277558327,
-0.02248551696538925,
-0.01056819036602974,
0.06672821193933487,
0.06678242981433868,
0.00042686285451054573,
-0.06549281626939774,
-0.018722154200077057,
-0.038233235478401184,
-0.04424809291958809,
0.023046506568789482,
-0.009073820896446705,
-0.013082140125334263,
-0.05316352844238281,
0.050927817821502686,
-0.04016027972102165,
0.06244751811027527,
0.011038331314921379,
0.042725805193185806,
0.015682509168982506,
-0.027811994776129723,
-0.013134869746863842,
0.018717600032687187,
-0.009752742946147919,
0.04117019101977348,
0.026355745270848274,
0.01333290059119463,
-0.0029257512651383877,
0.02086586318910122,
0.02177312970161438,
0.016126049682497978,
0.03350940719246864,
-0.04671844094991684,
-0.013168430887162685,
0.017698949202895164,
0.0022987721022218466,
0.08604668825864792,
-0.053368255496025085,
0.010168309323489666,
-0.3152167499065399,
0.009104747325181961,
-0.033283282071352005,
-0.08903484046459198,
-0.024398641660809517,
-0.029498262330889702,
-0.04353668913245201,
-0.057895585894584656,
-0.02187051996588707,
-0.03153588995337486,
0.06717594712972641,
0.03959699720144272,
-0.037202052772045135,
-0.0475010983645916,
0.053632985800504684,
0.06327087432146072,
0.0494341142475605,
-0.05866032838821411,
-0.029848141595721245,
0.0261039137840271,
-0.015383641235530376,
0.01642696000635624,
-0.02175614982843399,
-0.013792491517961025,
0.01089995726943016,
-0.03959307447075844,
0.10366666316986084,
-0.01172635518014431,
0.02985858917236328,
-0.017223984003067017,
0.011348232626914978,
-0.032910607755184174,
0.04073464870452881,
-0.0514051653444767,
-0.009763034991919994,
-0.05548800900578499,
0.013287932612001896,
-0.04689383506774902,
-0.06327305734157562,
0.030659925192594528,
-0.07908475399017334,
0.0011600833386182785,
-0.04061628505587578,
-0.0028320124838501215,
0.024336185306310654,
-0.01860859990119934,
0.019473601132631302,
0.05234340950846672,
-0.024335108697414398,
0.08742465078830719,
0.014767215587198734,
-0.03220387548208237,
0.006233393680304289,
0.05131574720144272,
0.028919562697410583,
-0.023065390065312386,
-0.08197793364524841,
0.05128445476293564,
-0.06418238580226898,
0.015423272736370564,
0.008120636455714703,
0.03901902213692665,
0.02149631828069687,
-0.04838626831769943,
0.011936010792851448,
0.03874604031443596,
0.0022726417519152164,
-0.03033357672393322,
0.039430126547813416,
-0.0034406876657158136,
-0.06396553665399551,
0.1082577034831047,
-0.018255753442645073,
-0.050459470599889755,
0.00941437017172575,
0.05334646627306938,
-0.018106883391737938,
-0.02419436350464821,
-0.028432516381144524,
0.03082209825515747,
0.024693265557289124,
-0.08313759416341782,
-0.004946364555507898,
0.05622635409235954,
-0.01220195833593607,
0.030299538746476173,
-0.06018802896142006,
0.032490137964487076,
-0.05082434043288231,
-0.041736796498298645,
0.000899975944776088,
-0.027194559574127197,
-0.03862561285495758,
0.015176570974290371,
-0.001250754576176405,
-0.007238470949232578,
-0.24825602769851685,
0.05684668570756912,
0.013454466126859188,
-0.04859590530395508,
0.007980630733072758,
0.023178961127996445,
-0.0055419378913939,
-0.03856049105525017,
0.023183392360806465,
0.012431815266609192,
0.07691176980733871,
-0.023008693009614944,
0.06955531984567642,
-0.00169284176081419,
-0.010463690385222435,
0.013206693343818188,
0.010940434411168098,
0.01715567149221897,
0.06811073422431946,
0.03494516387581825,
0.029726332053542137,
-0.05211219936609268,
0.11763313412666321,
-0.07546500861644745,
0.007835549302399158,
-0.005566884763538837,
0.04131339117884636,
0.09319828450679779,
0.003656005021184683,
0.02211947739124298,
0.040485311299562454,
-0.025364995002746582,
-0.0013717070687562227,
0.004623784217983484,
0.05289534106850624,
0.008855978026986122,
-0.04425068199634552,
-0.012873472645878792,
-0.03322698175907135,
0.0034332789946347475,
-0.05138375237584114,
-0.022830769419670105,
0.049451086670160294,
-0.03605758026242256,
0.08113022893667221,
0.005298512522131205,
-0.04633915796875954,
-0.01948266476392746,
0.019555196166038513,
-0.014882571995258331,
0.006348825991153717,
-0.029689308255910873,
0.03262495622038841,
0.036213468760252,
0.0030862041749060154,
-0.02253640815615654,
0.046843789517879486,
-0.008547142148017883,
0.046069417148828506,
-0.010507899336516857,
0.012273848056793213,
-0.07792665809392929,
-0.07047352939844131,
0.05241047218441963,
0.006978411693125963
] |
PMID:8206 | Malignant germ cell tumor in situ in a cryptorchid testis. | A case of a malignant undifferentiated germ cell tumor in situ of an undescended testis diagnosed by needle biopsy is described. Four similar cases have been so far recorded in the literature, all of them in the infertile men with normally descended descended testes. Two of them developed embryonal carcinoma of testis 4 1/2 later and one had a concomitant seminoma. In a high-risk group of patients (atrophic testis, cryptorchidism), a needle biopsy of a testicle may discover malignancy in its very early phase of development (Stage 0), at which time an orchiectomy alone may be a curative treatment. | Malignant germ cell tumor in situ in a cryptorchid testis. A case of a malignant undifferentiated germ cell tumor in situ of an undescended testis diagnosed by needle biopsy is described. Four similar cases have been so far recorded in the literature, all of them in the infertile men with normally descended descended testes. Two of them developed embryonal carcinoma of testis 4 1/2 later and one had a concomitant seminoma. In a high-risk group of patients (atrophic testis, cryptorchidism), a needle biopsy of a testicle may discover malignancy in its very early phase of development (Stage 0), at which time an orchiectomy alone may be a curative treatment. | [
0.0404207706451416,
0.017190903425216675,
0.020457394421100616,
-0.012306718155741692,
0.023985769599676132,
0.023354578763246536,
0.04018930718302727,
0.0013356386916711926,
-0.02424268238246441,
0.003646379103884101,
0.06792131811380386,
-0.016812454909086227,
0.06653428077697754,
0.005007500294595957,
-0.09942465275526047,
0.01694035902619362,
0.007152528967708349,
-0.005602182820439339,
-0.00027585841598920524,
0.04021941125392914,
0.046066638082265854,
-0.018171902745962143,
0.014756090007722378,
-0.04675336554646492,
-0.013751626014709473,
-0.010126431472599506,
-0.00802744273096323,
0.04416065663099289,
-0.05791393667459488,
-0.1396539956331253,
0.006746526341885328,
-0.04639240726828575,
0.005033643916249275,
-0.019711332395672798,
0.03743031248450279,
-0.03902893885970116,
0.04969565570354462,
0.0191758144646883,
0.034943707287311554,
0.00424608401954174,
0.028006725013256073,
-0.010707871988415718,
-0.029086269438266754,
0.03764660283923149,
0.006204469595104456,
0.02499309368431568,
-0.037064697593450546,
-0.034768134355545044,
0.07890748977661133,
0.02594255469739437,
0.00004695482493843883,
-0.05657578259706497,
-0.0043983738869428635,
0.0436825230717659,
-0.02883385866880417,
-0.066504567861557,
0.01975386030972004,
0.0438438355922699,
0.01167504396289587,
0.030490776523947716,
-0.007232227362692356,
0.05730273947119713,
-0.08277756720781326,
0.0046462565660476685,
0.05422528460621834,
-0.02331550046801567,
-0.05051190033555031,
-0.06907512992620468,
0.03554577752947807,
-0.06035023555159569,
0.059397052973508835,
0.04039360582828522,
0.04712718352675438,
0.1308162808418274,
-0.024074483662843704,
-0.06260988861322403,
-0.019339781254529953,
-0.016010373830795288,
-0.03510435298085213,
0.03538522124290466,
-0.04150666296482086,
0.024541735649108887,
0.005034548230469227,
-0.03391853719949722,
-0.06510330736637115,
-0.047093626111745834,
-0.0685742050409317,
-0.11566676944494247,
0.01822269707918167,
0.025570522993803024,
-0.0030740760266780853,
-0.03863339126110077,
-0.04459528997540474,
0.01990359276533127,
-0.053125541657209396,
0.006625209469348192,
-0.036499787122011185,
0.0010100564686581492,
0.0254205409437418,
0.36883801221847534,
-0.060753677040338516,
0.009577471762895584,
-0.00478461803868413,
-0.007099279668182135,
-0.05575032904744148,
0.010531939566135406,
-0.06245890259742737,
-0.0022062126081436872,
-0.011650645174086094,
0.06559731066226959,
0.0061262669041752815,
-0.00211585802026093,
0.07945116609334946,
0.004743690136820078,
-0.06261483579874039,
0.03341225907206535,
0.07180612534284592,
0.017927486449480057,
-0.08783066272735596,
0.06745678186416626,
0.052417632192373276,
-0.012721095234155655,
0.013142782263457775,
0.001506325206719339,
0.017515165731310844,
0.0037703656125813723,
0.0067287893034517765,
0.05258316919207573,
-0.0021455788519233465,
-0.022723974660038948,
0.010627704672515392,
0.0323198139667511,
-0.03252962604165077,
0.009128579869866371,
0.01095906738191843,
0.00453600101172924,
-0.03270820528268814,
-0.03050326555967331,
-0.05143864452838898,
-0.02086006850004196,
0.007141275331377983,
-0.1077197939157486,
-0.015417917631566525,
-0.006655985955148935,
0.016411449760198593,
0.0070334323681890965,
-0.0607406310737133,
-0.017375523224473,
-0.012703699059784412,
-0.00277356687001884,
-0.016002070158720016,
0.03609716519713402,
-0.04311349242925644,
0.029339831322431564,
-0.013335567899048328,
-0.04363230615854263,
-0.04016747698187828,
0.039202459156513214,
-0.03130774572491646,
-0.004505877383053303,
-0.0030895154923200607,
-0.04125608876347542,
-0.07253209501504898,
0.09877482801675797,
0.06803642213344574,
-0.05388127639889717,
-0.14975883066654205,
-0.011995086446404457,
0.00945120770484209,
-0.019801609218120575,
0.05160166695713997,
0.0057118427939713,
0.02736816368997097,
-0.016018381342291832,
-0.05833955481648445,
-0.038384679704904556,
-0.02714379131793976,
0.007301855832338333,
0.0024614178109914064,
0.04454153776168823,
0.04719991236925125,
-0.06389781832695007,
-0.023664776235818863,
0.06719792634248734,
-0.0339951366186142,
-0.06248157098889351,
-0.08239873498678207,
0.041201330721378326,
0.04174784943461418,
0.04472494125366211,
-0.025456992909312248,
-0.01734613999724388,
-0.05509644374251366,
0.0008505336591042578,
0.037717536091804504,
-0.05075563117861748,
0.005997869186103344,
-0.03397360444068909,
-0.04607412964105606,
-0.054410554468631744,
0.10485785454511642,
-0.006481279153376818,
0.07836856693029404,
0.07589388638734818,
-0.028966810554265976,
0.09879036247730255,
0.02360411547124386,
0.009121646173298359,
0.04173991084098816,
-0.003271962283179164,
-0.0488615520298481,
0.03314613923430443,
-0.009099802002310753,
0.027455540373921394,
0.005397888366132975,
0.0342513807117939,
-0.012587716802954674,
-0.010345390066504478,
0.04805682599544525,
-0.00018500027363188565,
0.030652901157736778,
-0.0059198359958827496,
0.06498142331838608,
-0.28429552912712097,
-0.06848236173391342,
-0.006463601719588041,
-0.030084675177931786,
-0.0016976724145933986,
-0.012358198873698711,
-0.03659190610051155,
0.01912103034555912,
0.027734683826565742,
-0.05889005959033966,
-0.009911445900797844,
0.09416993707418442,
-0.018350759521126747,
0.03148858994245529,
-0.0626400038599968,
0.030672617256641388,
0.0302801001816988,
-0.06465994566679001,
0.008560774847865105,
0.03152300789952278,
-0.03372019901871681,
0.02112806774675846,
-0.00814263429492712,
-0.02172709070146084,
0.08093616366386414,
-0.050936874002218246,
0.11941814422607422,
0.08117464184761047,
0.021370187401771545,
-0.030727505683898926,
0.054092612117528915,
-0.03457490727305412,
-0.017999712377786636,
-0.02572077512741089,
0.03430590033531189,
-0.08289193361997604,
-0.05442531034350395,
-0.005491502117365599,
-0.06778417527675629,
0.0051617929711937904,
-0.02742171101272106,
-0.05799936503171921,
-0.06419248878955841,
-0.04302738234400749,
0.018264446407556534,
-0.027485372498631477,
0.028727317228913307,
-0.004586769733577967,
0.03679041564464569,
0.04119570553302765,
0.0558726005256176,
-0.054737672209739685,
0.013470282778143883,
0.015977364033460617,
0.005776580888777971,
-0.023191401734948158,
-0.054187942296266556,
-0.04588286578655243,
-0.014026607386767864,
0.02303393930196762,
0.12104436010122299,
0.04182466119527817,
0.0017914780182763934,
-0.01910366490483284,
-0.012682732194662094,
-0.026521796360611916,
-0.016166772693395615,
0.009503770619630814,
0.049943938851356506,
-0.07157830148935318,
0.01847926899790764,
0.11259753257036209,
-0.017283925786614418,
0.04899246245622635,
0.031079132109880447,
0.0015770726604387164,
0.055304154753685,
0.01212706696242094,
0.008976847864687443,
-0.034211013466119766,
0.01164282113313675,
-0.03453848138451576,
0.03269124776124954,
-0.05885235592722893,
-0.024745633825659752,
0.05012323707342148,
-0.019238969311118126,
-0.0034130895510315895,
-0.011462340131402016,
-0.009974436834454536,
-0.016667691990733147,
0.06700693815946579,
-0.022508742287755013,
0.054423145949840546,
-0.055105287581682205,
-0.0026416226755827665,
-0.2658816874027252,
-0.028386181220412254,
-0.02967739664018154,
0.020802415907382965,
-0.02362927235662937,
0.038912639021873474,
0.01474676001816988,
0.018265405669808388,
-0.031130215153098106,
0.05844423174858093,
0.05668766051530838,
-0.04567936807870865,
0.06279091536998749,
-0.003066436620429158,
-0.014932254329323769,
0.01786038652062416,
0.06122482568025589,
-0.03726780787110329,
0.0004523455281741917,
-0.0684819296002388,
0.030980652198195457,
0.04150870814919472,
0.13442020118236542,
0.0094498535618186,
-0.02265026979148388,
0.02218015491962433,
0.026332801207900047,
0.07084223628044128,
0.016945380717515945,
0.01779613271355629,
0.06700991839170456,
0.017902908846735954,
-0.025384768843650818,
-0.014068606309592724,
0.02327045425772667,
0.02841120772063732,
0.0669531375169754,
0.08086669445037842,
0.034335460513830185,
0.01718273013830185,
-0.024835452437400818,
0.0004848506359849125,
-0.014747066423296928,
-0.05055595561861992,
0.03060939908027649,
0.0006527967634610832,
-0.05014119669795036,
-0.013179889880120754,
0.03252521529793739,
0.019534563645720482,
0.008409674279391766,
-0.0021894683595746756,
0.05117552727460861,
0.033249907195568085,
0.021379873156547546,
0.01026835199445486,
0.04681900143623352,
-0.05157758668065071,
0.020662102848291397,
-0.021461069583892822,
0.0395512580871582,
-0.03305518254637718,
-0.028679510578513145,
0.05786266177892685,
-0.039620961993932724
] |
PMID:8207 | Understanding neurotransmitters and related drugs. | Chemical neurotransmitter substances are released at the axon terminals of the central, autonomic, and peripheral nervous systems of the human body. The most well-known of the neurotransmitters are acetylcholine, norepinephrine, dopamine, and serotonin. It is these substances that facilitate the conduction of nerve impulses throughout the body, allowing the coordination of body functions and enabling response to the environment. The efective action of neurotransmitters makes the difference between health and disease states. A nurse's understanding of neurotransmitters and of many common drugs influencing their function is essential to safe nursing practice. | Understanding neurotransmitters and related drugs. Chemical neurotransmitter substances are released at the axon terminals of the central, autonomic, and peripheral nervous systems of the human body. The most well-known of the neurotransmitters are acetylcholine, norepinephrine, dopamine, and serotonin. It is these substances that facilitate the conduction of nerve impulses throughout the body, allowing the coordination of body functions and enabling response to the environment. The efective action of neurotransmitters makes the difference between health and disease states. A nurse's understanding of neurotransmitters and of many common drugs influencing their function is essential to safe nursing practice. | [
0.0018150147516280413,
-0.0224992074072361,
0.019288349896669388,
-0.01209825836122036,
0.01594635844230652,
0.028597868978977203,
0.1746610701084137,
0.010346234776079655,
-0.005011045373976231,
-0.06367473304271698,
-0.019524235278367996,
-0.021070871502161026,
-0.03428846225142479,
0.06480825692415237,
-0.010558120906352997,
0.012738142162561417,
-0.04063247889280319,
0.021140700206160545,
-0.03907762095332146,
-0.009539068676531315,
0.020098958164453506,
0.02009626105427742,
0.0582854300737381,
0.023328550159931183,
-0.015951476991176605,
0.011574816890060902,
-0.030003927648067474,
-0.03838665038347244,
-0.014563505537807941,
-0.10555295646190643,
-0.004546082578599453,
0.049709536135196686,
-0.04120568931102753,
-0.032586030662059784,
-0.017082609236240387,
0.01426059938967228,
-0.010840903036296368,
0.014868647791445255,
-0.01279418170452118,
-0.016771042719483376,
-0.002351190196350217,
-0.015688523650169373,
-0.002706873696297407,
-0.022013310343027115,
0.01585506461560726,
0.004871008917689323,
-0.028141554445028305,
-0.05963645130395889,
0.08748449385166168,
-0.07709681242704391,
0.04465125873684883,
0.039129145443439484,
0.044654279947280884,
0.06231531500816345,
0.014279987663030624,
-0.05925421789288521,
-0.005163798574358225,
0.031234879046678543,
-0.03385798633098602,
0.036865293979644775,
0.033078692853450775,
0.061788227409124374,
-0.08772191405296326,
0.04244457185268402,
0.05844620615243912,
-0.012190048582851887,
0.0018200288759544492,
0.02311594970524311,
0.0615367591381073,
0.014390564523637295,
-0.047209665179252625,
-0.01363114733248949,
-0.00864544976502657,
-0.012254900299012661,
-0.01091910619288683,
-0.04038899391889572,
0.019592763856053352,
-0.019831368699669838,
0.014187676832079887,
-0.03773840144276619,
0.08672624826431274,
0.01055875513702631,
0.05521755665540695,
-0.053123295307159424,
0.03687151148915291,
0.017141049727797508,
0.014408353716135025,
-0.022718895226716995,
-0.01990906149148941,
0.025001609697937965,
-0.062159474939107895,
-0.016945336014032364,
-0.022799331694841385,
0.028745904564857483,
-0.011719104833900928,
-0.04125680401921272,
0.0018676024628803134,
-0.032860420644283295,
-0.01564093492925167,
0.3603689968585968,
-0.012835674919188023,
-0.002063813153654337,
-0.04720813408493996,
-0.029590779915452003,
-0.01200549304485321,
-0.053147997707128525,
0.026655098423361778,
0.022441281005740166,
-0.0015360084362328053,
0.05727279186248779,
-0.0009575537987984717,
0.06340862810611725,
0.01657659187912941,
-0.0279267355799675,
0.00810411386191845,
-0.0711495503783226,
0.06090116873383522,
-0.006052336189895868,
0.06378182023763657,
0.0024720937944948673,
0.004116617143154144,
0.006779178511351347,
0.06304625421762466,
0.08039658516645432,
0.03699860721826553,
-0.013257320038974285,
-0.07973232865333557,
0.09597895294427872,
0.027356158941984177,
0.027718031778931618,
0.105441614985466,
0.026280004531145096,
0.023934660479426384,
0.03351171314716339,
-0.005363968200981617,
-0.0021043713204562664,
0.027022864669561386,
-0.0024540487211197615,
-0.01024414412677288,
-0.027809225022792816,
-0.035817112773656845,
-0.03025314025580883,
0.07365850359201431,
-0.13709230720996857,
-0.12284328788518906,
0.040901459753513336,
0.0025215032510459423,
-0.009263718500733376,
-0.0453873835504055,
0.07122838497161865,
-0.026402786374092102,
0.08757273107767105,
-0.027488823980093002,
0.04999804124236107,
0.0027109081856906414,
0.08212544023990631,
0.01566286012530327,
-0.08105616271495819,
-0.025384046137332916,
-0.020561225712299347,
-0.017662597820162773,
0.017549270763993263,
-0.013416343368589878,
0.08336585015058517,
0.013984558172523975,
0.012921445071697235,
-0.04101242870092392,
-0.03839131444692612,
0.020179951563477516,
-0.019531119614839554,
0.002861651359125972,
0.01797727309167385,
-0.04356616362929344,
-0.01095610298216343,
-0.0010529961436986923,
-0.016678014770150185,
0.03391188755631447,
-0.01638726145029068,
-0.016065960749983788,
-0.01612982526421547,
0.11441110074520111,
-0.03064155951142311,
0.015314359217882156,
0.01798391528427601,
0.04132740572094917,
0.020946720615029335,
-0.06867422163486481,
-0.0013736846158280969,
0.03708856552839279,
0.030333997681736946,
-0.058059949427843094,
0.020757850259542465,
-0.021845240145921707,
-0.02202613279223442,
-0.05175356566905975,
-0.03006187453866005,
-0.07789232581853867,
0.0011013135081157088,
-0.03986091911792755,
0.06469888240098953,
0.05557088181376457,
0.04460103437304497,
0.0051773726008832455,
0.09233367443084717,
-0.0065041808411479,
0.036213699728250504,
-0.02191818878054619,
0.0023421517107635736,
0.03464679419994354,
0.00850884523242712,
-0.0207658801227808,
0.012903310358524323,
0.022236069664359093,
-0.05274652689695358,
-0.044946398586034775,
-0.06417714804410934,
-0.010145381093025208,
0.030656857416033745,
0.08318053185939789,
0.024110563099384308,
0.04139629378914833,
-0.038788750767707825,
-0.04057515785098076,
-0.25266703963279724,
-0.0225277878344059,
0.05292508751153946,
-0.02700449526309967,
0.0544864758849144,
0.0026612768415361643,
0.02351291850209236,
-0.03815295919775963,
-0.03303324058651924,
-0.009439049288630486,
0.02132638543844223,
0.03954818472266197,
-0.04413104057312012,
-0.04727879911661148,
0.0001721366570563987,
0.008931674994528294,
0.02477661892771721,
-0.022158442065119743,
-0.06105797737836838,
-0.00026354967849329114,
0.030825218185782433,
-0.0695883259177208,
0.0028633554466068745,
-0.006796564441174269,
-0.035823628306388855,
0.0006241921219043434,
0.0985671654343605,
-0.01499319076538086,
0.016164323315024376,
-0.0373738668859005,
-0.0053949481807649136,
0.04476698860526085,
-0.033058665692806244,
-0.06960924714803696,
-0.025357140228152275,
-0.0979241281747818,
-0.015466704964637756,
0.011663665063679218,
-0.03355766832828522,
0.005434894002974033,
-0.042959533631801605,
-0.01642705872654915,
-0.01692255027592182,
0.022950418293476105,
-0.06752225011587143,
-0.002023473381996155,
-0.003158077597618103,
-0.09841229021549225,
-0.009711955673992634,
-0.06867922097444534,
0.035106126219034195,
0.058319125324487686,
0.012221574783325195,
-0.015664387494325638,
-0.026945935562253,
0.024652402848005295,
-0.04429994150996208,
0.011171792633831501,
-0.08846738934516907,
0.06283792108297348,
0.00009409302583662793,
0.03635569289326668,
-0.032571837306022644,
-0.06357917189598083,
0.016105419024825096,
0.015223730355501175,
0.027480237185955048,
0.04129811376333237,
0.05949385091662407,
-0.010356836952269077,
-0.005792060401290655,
0.14798259735107422,
0.005772218108177185,
0.04566975682973862,
0.05752414092421532,
-0.0033712375443428755,
-0.014545833691954613,
0.024052027612924576,
-0.07822412252426147,
0.061852797865867615,
-0.06375564634799957,
-0.021608073264360428,
0.053781937807798386,
0.018095292150974274,
0.024522272869944572,
-0.04326564073562622,
0.10365075618028641,
-0.039861101657152176,
0.046969301998615265,
0.033456265926361084,
-0.020438959822058678,
0.06227327510714531,
-0.014347093179821968,
-0.11236833781003952,
-0.03388424590229988,
0.05093252658843994,
-0.2506212294101715,
0.01911861076951027,
0.045779354870319366,
0.029402462765574455,
-0.03706786781549454,
-0.0061964658088982105,
-0.02687860280275345,
0.003013801295310259,
-0.05365907773375511,
0.04833913967013359,
0.046861425042152405,
0.02792486362159252,
0.014832095243036747,
0.027974624186754227,
-0.023175783455371857,
0.05944553390145302,
0.10770099610090256,
-0.06824586540460587,
0.011546013876795769,
-0.015399139374494553,
0.01912020519375801,
0.02353426069021225,
0.15218567848205566,
0.004690526984632015,
0.0041130040772259235,
0.03504978492856026,
-0.02106887474656105,
-0.02425086684525013,
0.0165876355022192,
-0.02852943353354931,
0.0430157296359539,
0.020294692367315292,
0.03677064925432205,
-0.02147439308464527,
0.04664050415158272,
0.036156028509140015,
-0.022837582975625992,
-0.02858305163681507,
-0.022023765370249748,
-0.010672660544514656,
-0.0583871528506279,
-0.029721172526478767,
-0.05679066479206085,
-0.03484621271491051,
0.06648548692464828,
-0.055215984582901,
-0.09714095294475555,
-0.1026059091091156,
0.014212935231626034,
0.011503975838422775,
-0.005739783402532339,
-0.011475778184831142,
-0.00985750276595354,
0.00348855834454298,
0.031473204493522644,
-0.01412970945239067,
-0.01978442631661892,
-0.08769474923610687,
0.030623193830251694,
0.01413742732256651,
-0.0773756355047226,
0.002454114379361272,
-0.001864595920778811,
0.06285201013088226,
0.029573868960142136
] |
PMID:8208 | Muscular work and the release of prostaglandin-like substances. | Experimental evidence for the release of prostaglandin-like substances, mainly of the E type, into the venous outflow from working skeletal muscles in the dog is described. Muscular exercise of the hind-leg produced by sciatic nerve stimulation evoked the release of prostaglandin-like substances detected in femoral venous blood by the bioassay method. This release occurred during and after muscular work, was abolished by indomethacin, and was not present in gallamine-treated dogs. The results suggest that endogenous vasodilator prostaglandins released during and after muscular work may contribute to local hyperaemic response during and, mainly, after muscular activity. | Muscular work and the release of prostaglandin-like substances. Experimental evidence for the release of prostaglandin-like substances, mainly of the E type, into the venous outflow from working skeletal muscles in the dog is described. Muscular exercise of the hind-leg produced by sciatic nerve stimulation evoked the release of prostaglandin-like substances detected in femoral venous blood by the bioassay method. This release occurred during and after muscular work, was abolished by indomethacin, and was not present in gallamine-treated dogs. The results suggest that endogenous vasodilator prostaglandins released during and after muscular work may contribute to local hyperaemic response during and, mainly, after muscular activity. | [
-0.017305618152022362,
0.033328231424093246,
-0.008224357850849628,
-0.006112685427069664,
0.016438137739896774,
0.0022500704508274794,
0.02377307042479515,
-0.019865820184350014,
-0.0018244857201352715,
-0.026424990966916084,
0.007875676266849041,
-0.026322761550545692,
-0.04121973365545273,
0.05031101405620575,
0.0019102649530395865,
-0.015002595260739326,
0.061131540685892105,
0.027847789227962494,
-0.08376888930797577,
-0.014169413596391678,
-0.01718752086162567,
-0.01103199366480112,
0.007379639893770218,
-0.010868147946894169,
0.0356774665415287,
0.019788499921560287,
-0.026472417637705803,
0.012151422910392284,
0.005208705086261034,
-0.1376904845237732,
0.023819496855139732,
-0.02290530502796173,
-0.025830399245023727,
0.0277574323117733,
0.039758581668138504,
-0.007364385761320591,
-0.0699969008564949,
-0.029064277186989784,
-0.06229432672262192,
0.046500734984874725,
0.09698967635631561,
-0.008623961359262466,
-0.038313038647174835,
0.04652272164821625,
0.09990710765123367,
0.08501607924699783,
-0.00394001929089427,
-0.020468588918447495,
0.08380517363548279,
0.010870929807424545,
0.07713203132152557,
0.023373985663056374,
0.04777445271611214,
0.0487128384411335,
0.025178948417305946,
-0.05868636444211006,
0.01523478515446186,
0.0178986769169569,
-0.06242876127362251,
0.003953995648771524,
0.018479391932487488,
0.10847495496273041,
-0.1552324891090393,
0.07056816667318344,
0.03216512128710747,
-0.00442380690947175,
-0.013501853682100773,
-0.01904664933681488,
0.05723702535033226,
0.07771662622690201,
-0.04223543778061867,
0.08830045908689499,
-0.04608471319079399,
-0.01980193704366684,
0.022698339074850082,
-0.004208400845527649,
-0.016779949888586998,
0.003915185574442148,
0.00978769175708294,
0.005420658737421036,
0.011371510103344917,
0.018505407497286797,
0.03013475239276886,
-0.012670201249420643,
-0.05224921926856041,
-0.02446136064827442,
0.04204317927360535,
-0.002288163872435689,
0.07378271222114563,
0.03354873135685921,
-0.08243943005800247,
0.004118674900382757,
-0.05628880485892296,
0.012083918787539005,
-0.038042549043893814,
-0.001713956007733941,
-0.01603075861930847,
0.02830016054213047,
0.011992201209068298,
0.37873387336730957,
-0.07820334285497665,
0.01396007090806961,
-0.011136449873447418,
-0.015385624952614307,
0.03720973804593086,
-0.009687989018857479,
-0.02223809063434601,
0.02116541750729084,
-0.051872652024030685,
0.04406081885099411,
0.06906870007514954,
0.03904451057314873,
0.03161351755261421,
0.011354440823197365,
-0.0018634275766089559,
-0.003640140173956752,
-0.007083966862410307,
-0.017244217917323112,
-0.015716329216957092,
0.00005277225864119828,
-0.06202121451497078,
-0.029510747641324997,
0.006410118192434311,
0.01077333465218544,
0.014941563829779625,
0.003705295966938138,
0.016411691904067993,
0.05312192440032959,
0.04687429592013359,
-0.05413538217544556,
0.03948022052645683,
0.006487151142209768,
-0.019880667328834534,
0.0022569033317267895,
0.019404161721467972,
-0.03638197481632233,
0.10963718593120575,
0.047646183520555496,
-0.003760449355468154,
-0.01012697909027338,
-0.03157256171107292,
-0.04021211713552475,
0.01525039877742529,
-0.10519511997699738,
-0.02718932367861271,
0.09741484373807907,
0.07090884447097778,
-0.03122948855161667,
-0.012287803925573826,
-0.0015878697158768773,
-0.022914059460163116,
0.04640079289674759,
-0.009030632674694061,
0.03740549087524414,
0.008751912973821163,
0.030104834586381912,
0.03331852704286575,
-0.02119356393814087,
-0.011081964708864689,
0.012497130781412125,
-0.05193185433745384,
-0.05484233796596527,
-0.032940588891506195,
0.012903375551104546,
0.05949065089225769,
0.013630487024784088,
-0.054344773292541504,
-0.040435440838336945,
0.0649411678314209,
0.03519171103835106,
-0.016408350318670273,
-0.00478149252012372,
-0.08160736411809921,
-0.036674775183200836,
-0.0003986126685049385,
0.02955874800682068,
0.003749665804207325,
-0.02577076479792595,
0.031299248337745667,
0.01343710906803608,
0.10488218814134598,
-0.05222730338573456,
0.0033202467020601034,
0.02025984786450863,
0.013232460245490074,
0.035951387137174606,
-0.08625233173370361,
0.012528437189757824,
-0.00022232209448702633,
0.0632365271449089,
-0.0010851100087165833,
-0.040698278695344925,
-0.05588017404079437,
-0.09420794993638992,
-0.019656669348478317,
-0.07034537196159363,
-0.048698440194129944,
-0.010165886022150517,
-0.054298024624586105,
-0.02636089362204075,
0.0488138310611248,
-0.017616327852010727,
0.010543085634708405,
0.04821716621518135,
-0.00946599431335926,
0.07510624080896378,
-0.0317234992980957,
-0.018469911068677902,
0.007675322704017162,
0.0437793992459774,
0.013788203708827496,
0.007515592500567436,
0.03282807767391205,
-0.0027273071464151144,
-0.04444844275712967,
0.02247985452413559,
-0.022641120478510857,
-0.01000452134758234,
0.028296275064349174,
-0.009675644338130951,
-0.014173761941492558,
-0.05117308720946312,
0.023585395887494087,
-0.2905843257904053,
-0.03738857060670853,
0.004414469003677368,
-0.02871054783463478,
0.0424114428460598,
0.026500722393393517,
0.006963621359318495,
-0.061741847544908524,
0.050870027393102646,
-0.05167475342750549,
0.04448379948735237,
0.08713053911924362,
-0.08921536058187485,
-0.04244799539446831,
0.07395615428686142,
0.10843949764966965,
0.02973811700940132,
-0.0627451241016388,
-0.030994674190878868,
0.013344723731279373,
0.052518632262945175,
-0.039358291774988174,
-0.06493919342756271,
0.012487652711570263,
0.037286177277565,
-0.0269616786390543,
0.12660865485668182,
0.0008960781269706786,
-0.00006598713662242517,
-0.0478801354765892,
-0.013372649438679218,
0.03264932706952095,
-0.0187188982963562,
-0.10197467356920242,
-0.0036207560915499926,
-0.042535681277513504,
0.006592005956918001,
0.017742186784744263,
0.006251991726458073,
-0.007620608899742365,
-0.04881178215146065,
0.041334353387355804,
0.005821908824145794,
0.00002430483073112555,
-0.06988446414470673,
-0.03148210421204567,
0.005745286121964455,
-0.008038193918764591,
-0.07219453155994415,
0.003442242741584778,
0.05196480080485344,
0.00973543617874384,
0.03800446540117264,
-0.02695017121732235,
-0.011605991050601006,
-0.05977311730384827,
-0.03182140737771988,
-0.0026357450988143682,
-0.056178897619247437,
-0.02643086388707161,
0.014783202670514584,
0.000473797699669376,
0.04942045733332634,
-0.008512833155691624,
0.006036361679434776,
-0.007535772398114204,
0.007175877224653959,
0.024197516962885857,
0.06321118026971817,
0.01253446377813816,
0.025068333372473717,
0.12080682069063187,
-0.04384371265769005,
0.001735932077281177,
0.08600615710020065,
-0.017420180141925812,
-0.007781635504215956,
-0.01939813606441021,
-0.06481454521417618,
0.02495465613901615,
-0.0036722247023135424,
-0.0454195998609066,
0.02436370775103569,
0.0023192616645246744,
0.0057020592503249645,
-0.004645583685487509,
0.003260083496570587,
-0.011403641663491726,
0.04061076417565346,
0.02820412442088127,
0.01176253892481327,
0.009830709546804428,
-0.016895657405257225,
-0.011781387962400913,
0.011607665568590164,
0.03984866291284561,
-0.2726910412311554,
0.008980180136859417,
0.08657804876565933,
-0.045428767800331116,
-0.03594660758972168,
0.03419133275747299,
-0.0522066168487072,
-0.03354322537779808,
-0.038811810314655304,
0.02711517922580242,
0.01992191933095455,
0.02228434570133686,
-0.009727630764245987,
0.057341404259204865,
-0.05687107518315315,
0.021034911274909973,
0.05577770248055458,
-0.038081180304288864,
0.03452148661017418,
-0.05502309277653694,
-0.02127605304121971,
-0.06124362349510193,
0.13213354349136353,
0.04018368571996689,
0.013274864293634892,
0.004086831118911505,
0.02842964231967926,
-0.013094797730445862,
-0.023563668131828308,
-0.010912188328802586,
0.03469115495681763,
-0.013447406701743603,
0.06421387940645218,
-0.009026575833559036,
-0.007513514719903469,
-0.011527498252689838,
-0.01482833456248045,
0.04547852277755737,
-0.015112029388546944,
0.006634140852838755,
0.0013079295167699456,
-0.024228405207395554,
-0.045095086097717285,
-0.03809339180588722,
0.060638491064310074,
-0.0661032646894455,
-0.06122829392552376,
-0.06810438632965088,
0.061500150710344315,
-0.025987159460783005,
-0.0013148682191967964,
-0.06903810799121857,
0.0008865504059940577,
0.06169678270816803,
-0.006704035680741072,
-0.02198978327214718,
0.030727989971637726,
-0.009190280921757221,
-0.004683654755353928,
0.0176314078271389,
-0.002897776896134019,
-0.047795381397008896,
-0.06433910876512527,
0.09329786896705627,
0.01246891263872385
] |
PMID:8210 | Fine structure of complex ocelli of a cubomedusan, Tamoya bursaria Haeckel. | The retina of the distal and proximal lens-bearing complex ocelli are composed of pigmented sensory cells and long pigmented cells. A ciliary sheath from each sensory cell, together with the processes of long pigmented cells, extends through the vitreous layer as far as the capsule that envelops the lens. Each ciliary sheath has several ballon-like swellings and the ciliary microtubules, arranged in the 9+2 pattern in the proximal part, are markedly disorganized distally in the swollen parts, out of which extends most of the microvilli in the vitreous layer. It is suggested that some of the microvilli may originate in vesicles that are constricted off from the surface of the pigmented sensory cells. Closely packed microvilli run in parallel in short bundles. In addition to characteristic junctions between sensory cells, junctions that are presumably synaptic and, of a new type in coelenterates, are observed between sensory cells and nerve endings. | Fine structure of complex ocelli of a cubomedusan, Tamoya bursaria Haeckel. The retina of the distal and proximal lens-bearing complex ocelli are composed of pigmented sensory cells and long pigmented cells. A ciliary sheath from each sensory cell, together with the processes of long pigmented cells, extends through the vitreous layer as far as the capsule that envelops the lens. Each ciliary sheath has several ballon-like swellings and the ciliary microtubules, arranged in the 9+2 pattern in the proximal part, are markedly disorganized distally in the swollen parts, out of which extends most of the microvilli in the vitreous layer. It is suggested that some of the microvilli may originate in vesicles that are constricted off from the surface of the pigmented sensory cells. Closely packed microvilli run in parallel in short bundles. In addition to characteristic junctions between sensory cells, junctions that are presumably synaptic and, of a new type in coelenterates, are observed between sensory cells and nerve endings. | [
-0.02294795773923397,
-0.08013830333948135,
0.002121654339134693,
0.05914077162742615,
0.02503318339586258,
0.0010610023746266961,
0.051007844507694244,
-0.019605878740549088,
-0.009128854610025883,
-0.01686829701066017,
-0.002891161944717169,
-0.09199649840593338,
0.0663408413529396,
0.039281249046325684,
0.03061603009700775,
-0.019267335534095764,
0.006270027253776789,
0.002349704969674349,
-0.0701608881354332,
-0.0006524650380015373,
0.009694463573396206,
-0.047645170241594315,
-0.006513349711894989,
-0.010429184883832932,
-0.007049959152936935,
0.0067859431728720665,
0.006597244180738926,
-0.03604044392704964,
-0.0022952710278332233,
-0.2289075255393982,
-0.028260063380002975,
0.030334515497088432,
-0.008620991371572018,
-0.020078692585229874,
0.01948319934308529,
-0.06067531928420067,
-0.01213640533387661,
0.004210804123431444,
0.0025565545074641705,
0.05041223391890526,
0.05496912822127342,
-0.0034810209181159735,
-0.02970261313021183,
0.04676457867026329,
0.02613329328596592,
-0.08519057929515839,
-0.014273509383201599,
0.008460158482193947,
0.07836849987506866,
-0.018221326172351837,
-0.04135894030332565,
-0.06822656840085983,
-0.008936270140111446,
0.0861138105392456,
-0.026211682707071304,
0.05096840858459473,
-0.006595564540475607,
0.006298796273767948,
0.03667304292321205,
0.004852799233049154,
0.05426332354545593,
0.03781754523515701,
-0.07095037400722504,
0.08260799944400787,
0.02206720970571041,
-0.0007058045011945069,
-0.011169420555233955,
-0.02017120085656643,
0.06591357290744781,
-0.008089577779173851,
0.02141799032688141,
0.006909599062055349,
0.02113102190196514,
-0.05936112627387047,
0.009383094497025013,
-0.024656064808368683,
-0.010228442028164864,
-0.04148253798484802,
-0.03784889727830887,
0.08531133085489273,
-0.04507892206311226,
0.01016611885279417,
0.016837799921631813,
0.008912996388971806,
0.020585140213370323,
0.030240802094340324,
-0.05983101576566696,
-0.05069902539253235,
-0.022242333739995956,
0.008303297683596611,
-0.0315898060798645,
-0.07253390550613403,
-0.07064514607191086,
0.004447442013770342,
-0.0972866341471672,
-0.0330849252641201,
-0.03840281441807747,
-0.038870926946401596,
0.02545531652867794,
0.3382083773612976,
-0.025520434603095055,
-0.03742389753460884,
0.03742092475295067,
-0.03919859230518341,
0.009949916042387486,
0.04587806761264801,
-0.007946064695715904,
-0.035095904022455215,
-0.015248710289597511,
0.09202238917350769,
0.008958486840128899,
0.008184166625142097,
-0.04690712317824364,
0.017823953181505203,
-0.007290299516171217,
-0.012959858402609825,
0.056400496512651443,
-0.0291097704321146,
-0.002286643721163273,
0.028994984924793243,
-0.040495239198207855,
0.003121744841337204,
0.02358502894639969,
0.036396875977516174,
0.016977490857243538,
0.07252921164035797,
-0.05092129856348038,
0.039583656936883926,
0.0255857203155756,
-0.03026597760617733,
0.008241645060479641,
0.09100497514009476,
0.01768922246992588,
-0.0491812638938427,
0.0014130312483757734,
0.06484785676002502,
0.05111641064286232,
-0.07150890678167343,
0.005844578612595797,
-0.011012513190507889,
-0.04880606010556221,
-0.10595648735761642,
0.009217791259288788,
-0.06592920422554016,
-0.01825758069753647,
0.07082125544548035,
-0.02300878055393696,
0.004841713700443506,
-0.01177318673580885,
0.0619354210793972,
0.004231962841004133,
0.047247257083654404,
0.023176783695816994,
0.03889884799718857,
0.036970026791095734,
0.03287598490715027,
-0.049898408353328705,
0.030151350423693657,
0.017371058464050293,
-0.001584845595061779,
-0.0281910989433527,
-0.02360633760690689,
0.02803318202495575,
0.10576675087213516,
0.05341539531946182,
-0.07249633222818375,
-0.007117760833352804,
-0.015334803611040115,
0.006160821299999952,
-0.059674281626939774,
-0.027173392474651337,
-0.002076779492199421,
0.03774433955550194,
0.03991207107901573,
-0.010875417850911617,
0.03947727009654045,
-0.06478049606084824,
-0.03439848870038986,
-0.002687089378014207,
-0.015072962269186974,
0.045697323977947235,
-0.0070779151283204556,
-0.04770929738879204,
0.004534462932497263,
0.022098246961832047,
-0.009714680723845959,
-0.01240613404661417,
0.01836804486811161,
0.0016702540451660752,
-0.015329905785620213,
-0.03477112576365471,
0.012480654753744602,
-0.02153407223522663,
0.050476476550102234,
-0.04242749139666557,
-0.030443673953413963,
0.03724917769432068,
-0.007960227318108082,
-0.00021363921405281872,
-0.049538180232048035,
0.11792906373739243,
-0.00941823422908783,
-0.060500383377075195,
0.09284349530935287,
0.015799378976225853,
-0.014448513276875019,
0.05462656542658806,
0.031171180307865143,
0.02788659930229187,
0.022317958995699883,
-0.03096260502934456,
-0.02542804554104805,
0.014166465029120445,
-0.014757207594811916,
0.008219115436077118,
0.005687342025339603,
0.03112839348614216,
0.009031352587044239,
0.04297323152422905,
-0.02528649941086769,
0.045082345604896545,
-0.10441436618566513,
-0.09507609903812408,
-0.2858343720436096,
0.03740931302309036,
-0.0506892204284668,
-0.044163115322589874,
0.004713850095868111,
-0.06569435447454453,
0.058202218264341354,
-0.02881593257188797,
0.0065816789865493774,
-0.10844037681818008,
0.07294943928718567,
0.02323688380420208,
0.01796613447368145,
-0.02188253402709961,
-0.0013717478141188622,
0.048213716596364975,
0.028314877301454544,
-0.025470007210969925,
0.02806105837225914,
0.04120977967977524,
0.031990304589271545,
-0.0378754585981369,
-0.008919307962059975,
0.08120772242546082,
0.0615847148001194,
-0.06720510870218277,
0.1373032033443451,
-0.007490826770663261,
-0.004221828188747168,
-0.051666658371686935,
-0.02311656065285206,
0.0498049221932888,
0.021296966820955276,
-0.01607109233736992,
0.029019201174378395,
0.02836298570036888,
0.01543107908219099,
-0.05256396904587746,
0.007842128165066242,
0.011376217007637024,
0.023433320224285126,
0.015695227310061455,
-0.05613863840699196,
-0.048684652894735336,
0.02051880583167076,
0.030167831107974052,
0.033371299505233765,
0.0020977510139346123,
0.021636471152305603,
-0.024594079703092575,
0.030022840946912766,
-0.019411522895097733,
0.023576607927680016,
0.031212950125336647,
0.007030421867966652,
0.006251291371881962,
-0.05036073923110962,
-0.01756897196173668,
-0.08866126835346222,
0.022373033687472343,
-0.0039050609339028597,
0.007982341572642326,
-0.04402139037847519,
-0.0362410694360733,
0.02410557121038437,
0.03745390847325325,
0.015090222470462322,
-0.02478848211467266,
0.08063953369855881,
-0.06415939331054688,
0.020944153890013695,
0.12907621264457703,
-0.03205624222755432,
-0.053582772612571716,
0.044138647615909576,
0.029845038428902626,
0.04749979078769684,
-0.020879549905657768,
0.04210003465414047,
0.009631692431867123,
0.016063125804066658,
-0.02975565195083618,
0.016927745193243027,
0.008041072636842728,
-0.018025586381554604,
-0.014005529694259167,
-0.015799183398485184,
-0.06868614256381989,
0.015715209767222404,
-0.04685695096850395,
0.018618764355778694,
0.04518243297934532,
-0.027801504358649254,
-0.0005443045520223677,
0.002057313919067383,
0.028059212490916252,
-0.28649991750717163,
0.07154951989650726,
0.06851180642843246,
0.009932070970535278,
0.0003792895295191556,
0.04884244129061699,
0.020293161273002625,
-0.04232214391231537,
0.0572236143052578,
0.02962731570005417,
0.05744355916976929,
0.017035605385899544,
0.03439072519540787,
0.05980918928980827,
0.009415222331881523,
0.0397900752723217,
0.07770597189664841,
-0.023643063381314278,
0.05691233649849892,
0.02582392655313015,
-0.035182707011699677,
-0.02891380898654461,
0.13064511120319366,
-0.013539972715079784,
-0.006050759460777044,
-0.04621104896068573,
0.01405171025544405,
0.0021694633178412914,
-0.024981414899230003,
0.02640390396118164,
0.04221993684768677,
0.047810133546590805,
0.0023809405975043774,
0.0014268901431933045,
-0.01153344102203846,
0.012909773737192154,
0.028805062174797058,
0.024148577824234962,
-0.010659394785761833,
-0.000527107622474432,
0.05737083777785301,
-0.05798032134771347,
-0.10578155517578125,
-0.040587328374385834,
0.03966715559363365,
-0.018495557829737663,
-0.061701852828264236,
0.021301619708538055,
-0.018799960613250732,
0.007797555532306433,
-0.08428695797920227,
-0.06357178092002869,
-0.021733498200774193,
0.05207306519150734,
-0.05013249069452286,
-0.022905323654413223,
0.0005908513558097184,
-0.04424421861767769,
0.01571035012602806,
-0.0046938201412558556,
-0.0006254832842387259,
-0.09649638086557388,
0.0046256366185843945,
0.008479176089167595,
0.04588524252176285
] |
PMID:8211 | The mechanism of action of ppGpp on rRNA synthesis in vitro. | We have studied the mechanism of the specific inhibition of ribosomal RNA synthesis by ppGpp in a purified system using as templates E. coli DNA and DNA from lambdad5ilv, which carries a rRNA cistron from E. coli. Ribosomal RNA synthesis, as well as its inhibition by ppGpp, are critically salt-dependent. Of a number of guanosine phosphates tested, only pppGpp (MS II) mimicked the action of ppGpp, establishing the specificity of ppGpp. The two templates gave similar results for rRNA synthesis in all experiments. By using the initiation inhibitor rifampicin, we could show that the specific inhibition of rRNA synthesis by ppGpp is due to its effect on rRNA initiation. The somewhat variable inhibition of RNA synthesis in general by ppGpp is mainly or wholly a consequence of premature chain termination. We propose that ppGpp specifically inhibits rRNA synthesis by acting on the formation of the so-called "closed-promoter complex". | The mechanism of action of ppGpp on rRNA synthesis in vitro. We have studied the mechanism of the specific inhibition of ribosomal RNA synthesis by ppGpp in a purified system using as templates E. coli DNA and DNA from lambdad5ilv, which carries a rRNA cistron from E. coli. Ribosomal RNA synthesis, as well as its inhibition by ppGpp, are critically salt-dependent. Of a number of guanosine phosphates tested, only pppGpp (MS II) mimicked the action of ppGpp, establishing the specificity of ppGpp. The two templates gave similar results for rRNA synthesis in all experiments. By using the initiation inhibitor rifampicin, we could show that the specific inhibition of rRNA synthesis by ppGpp is due to its effect on rRNA initiation. The somewhat variable inhibition of RNA synthesis in general by ppGpp is mainly or wholly a consequence of premature chain termination. We propose that ppGpp specifically inhibits rRNA synthesis by acting on the formation of the so-called "closed-promoter complex". | [
-0.07008138298988342,
-0.01004945207387209,
-0.04959310591220856,
-0.034430015832185745,
0.0007591860485263169,
0.02886918932199478,
-0.01845603808760643,
0.0069533600471913815,
-0.06139511615037918,
0.010222239419817924,
0.045674461871385574,
-0.004760617855936289,
0.03877530246973038,
-0.008679517544806004,
-0.04606515169143677,
-0.0015166756929829717,
-0.06608868390321732,
-0.01116536371409893,
-0.006244503892958164,
0.03787516802549362,
0.01597520336508751,
0.04489036276936531,
-0.03201674297451973,
-0.06079154089093208,
-0.01827482506632805,
0.032107602804899216,
0.02794383466243744,
0.007438643369823694,
-0.02945231832563877,
-0.23631145060062408,
-0.008757690899074078,
0.006798336748033762,
0.01631547138094902,
-0.08467532694339752,
-0.029170673340559006,
-0.007263094652444124,
-0.06511348485946655,
-0.007846604101359844,
-0.06884375214576721,
0.08455947786569595,
-0.007762061432003975,
0.036720432341098785,
0.007733136415481567,
0.02124721184372902,
0.06872370839118958,
-0.02184303291141987,
0.01684585213661194,
-0.006774336099624634,
0.04404515027999878,
-0.03823483735322952,
-0.018204718828201294,
-0.017297061160206795,
-0.05076739564538002,
-0.01949775218963623,
-0.02787809446454048,
0.027691848576068878,
0.058059513568878174,
0.04182577505707741,
-0.00522759510204196,
-0.02023109793663025,
-0.03639836609363556,
0.03091508336365223,
-0.14933447539806366,
0.030918056145310402,
0.05229024216532707,
0.02422013320028782,
0.010695644654333591,
-0.005395852494984865,
0.02360440231859684,
0.036614056676626205,
-0.06992244720458984,
0.06046615540981293,
-0.03183169290423393,
0.048110730946063995,
0.00041692532249726355,
-0.011893406510353088,
-0.05051466450095177,
0.02562444657087326,
0.06202312931418419,
0.05926701799035072,
-0.00040575003367848694,
0.007381891366094351,
0.005943060386925936,
0.002621280262246728,
-0.04083488509058952,
0.03641452267765999,
0.010220853611826897,
-0.05025705322623253,
-0.01762494258582592,
-0.00901977252215147,
0.05005652457475662,
-0.04963957518339157,
0.014765070751309395,
-0.032037943601608276,
-0.05956263840198517,
0.07100602239370346,
0.027627045288681984,
-0.024455510079860687,
0.006993087474256754,
0.3472939431667328,
-0.07144530117511749,
0.018491366878151894,
-0.0639323741197586,
0.018293336033821106,
0.04058825597167015,
-0.018196694552898407,
0.030870363116264343,
0.027182364836335182,
-0.00463819969445467,
0.06035201996564865,
0.03485751524567604,
0.023351967334747314,
-0.021181199699640274,
0.004008768126368523,
0.03467067331075668,
-0.004591871984302998,
0.027416467666625977,
0.016670117154717445,
-0.10044338554143906,
0.031423669308423996,
-0.012877479195594788,
-0.04516740143299103,
0.014993780292570591,
0.026176877319812775,
0.006906460504978895,
-0.015220536850392818,
-0.05255225673317909,
0.09855152666568756,
-0.002870948053896427,
0.01943444460630417,
0.07990763336420059,
0.018483594059944153,
-0.016158461570739746,
0.036690302193164825,
0.02738458849489689,
-0.04027295112609863,
0.03481624647974968,
-0.023688307031989098,
0.04700654745101929,
-0.03920779377222061,
-0.01229835208505392,
0.003334029810503125,
-0.08088117837905884,
-0.036176640540361404,
-0.019006716087460518,
0.07399886101484299,
-0.024449767544865608,
0.06452396512031555,
0.014221027493476868,
0.01055117230862379,
-0.01838945411145687,
0.04391136020421982,
0.021511180326342583,
0.006880559958517551,
-0.03111458197236061,
0.007880734279751778,
-0.01018491666764021,
-0.001725505106151104,
0.011499513871967793,
-0.01792174205183983,
-0.09457188099622726,
-0.01673058420419693,
0.009863086976110935,
0.06815138459205627,
-0.036414578557014465,
-0.040999118238687515,
0.004271169193089008,
0.04370122402906418,
-0.009551838971674442,
-0.042722418904304504,
-0.0567544586956501,
-0.011493762023746967,
0.035134606063365936,
-0.08840454369783401,
-0.03619283810257912,
0.007882238365709782,
-0.012862497940659523,
-0.011053512804210186,
0.036552585661411285,
-0.015863481909036636,
-0.00999562256038189,
0.019690122455358505,
0.010686595924198627,
0.0002372485469095409,
-0.00691722147166729,
-0.07656769454479218,
-0.04385757818818092,
-0.01705228164792061,
0.03429969400167465,
-0.0006444217287935317,
-0.02012467570602894,
-0.000874458986800164,
-0.11366257816553116,
0.024273378774523735,
-0.03194427117705345,
-0.0700201690196991,
-0.01864635944366455,
0.05209939554333687,
-0.020194172859191895,
0.00559990294277668,
0.09791261702775955,
0.04511215537786484,
0.000008709673238627147,
0.02755611762404442,
-0.056912947446107864,
0.005046224687248468,
0.015242597088217735,
0.009580647572875023,
0.0031119072809815407,
0.006170556414872408,
-0.01949279196560383,
0.008932674303650856,
-0.05001559108495712,
0.04010864719748497,
-0.07092725485563278,
0.0282057523727417,
-0.009545045904815197,
0.07475991547107697,
0.03494285047054291,
0.05598439276218414,
0.012538055889308453,
-0.04013684391975403,
-0.028028221800923347,
-0.2916669249534607,
-0.021407654508948326,
-0.01943761110305786,
-0.007728449534624815,
0.09469810128211975,
-0.014696658588945866,
0.00035175285302102566,
0.031810641288757324,
-0.02741909772157669,
-0.029481135308742523,
0.07078006863594055,
0.0881023034453392,
-0.0035775129217654467,
-0.015810944139957428,
-0.031062105670571327,
-0.014937550760805607,
-0.008711211383342743,
0.02377147786319256,
0.015877576544880867,
-0.0373011976480484,
0.03720057010650635,
-0.03156578168272972,
-0.04524300992488861,
-0.03734496980905533,
0.021770557388663292,
0.01473455410450697,
0.0967562198638916,
0.054449412971735,
0.023243006318807602,
0.027777932584285736,
0.007528927642852068,
0.04860081523656845,
-0.02766588144004345,
-0.12311775982379913,
-0.019710445776581764,
0.057363636791706085,
0.0044860802590847015,
-0.0007803432526998222,
0.0682477131485939,
-0.01976388692855835,
0.03048701211810112,
0.07228171080350876,
-0.0433318093419075,
-0.07033109664916992,
-0.030161773785948753,
-0.00981426052749157,
-0.005126119591295719,
0.05019046366214752,
0.07248350232839584,
0.024173114448785782,
0.015194343402981758,
0.04545467346906662,
0.07669898122549057,
0.015236510895192623,
0.019833628088235855,
-0.03234283998608589,
-0.008237512782216072,
-0.013105368241667747,
-0.0462484285235405,
-0.002015839098021388,
0.005441057961434126,
-0.03632141649723053,
-0.000896965735591948,
0.04480747878551483,
0.02551242709159851,
-0.018888160586357117,
-0.049782101064920425,
0.025371897965669632,
0.10601908713579178,
0.060184504836797714,
0.04163808375597,
0.12497112900018692,
-0.0007808876107446849,
0.017536520957946777,
0.026107368990778923,
0.023278402164578438,
-0.057786695659160614,
-0.10426260530948639,
-0.05516605079174042,
-0.06207148730754852,
-0.01640407368540764,
0.018201783299446106,
0.022011389955878258,
-0.07929497212171555,
-0.008232234045863152,
-0.0288644190877676,
0.011221714317798615,
0.004916921257972717,
0.062358930706977844,
-0.006328541785478592,
0.01935456320643425,
-0.01940884441137314,
-0.04022489860653877,
-0.010986781679093838,
0.058987490832805634,
0.02744525857269764,
-0.279253214597702,
-0.021988099440932274,
-0.02216283418238163,
0.01343047246336937,
-0.017862839624285698,
0.07097973674535751,
0.019452029839158058,
-0.08138757199048996,
-0.040542516857385635,
0.030751027166843414,
0.03200811892747879,
0.05366143211722374,
0.04569478705525398,
0.07507287710905075,
0.02193588577210903,
0.037833429872989655,
0.0301031693816185,
-0.044017937034368515,
0.017206916585564613,
-0.043276235461235046,
-0.0006441515870392323,
-0.027988629415631294,
0.1477205455303192,
0.029124319553375244,
0.020222363993525505,
-0.022696668282151222,
0.03054380975663662,
0.0027307358104735613,
-0.02330975979566574,
0.0249372199177742,
-0.03014969266951084,
0.016129078343510628,
0.06645912677049637,
-0.016740137711167336,
0.045436959713697433,
0.04954823851585388,
0.012933187186717987,
0.043761830776929855,
-0.029760142788290977,
-0.004833498038351536,
-0.042354099452495575,
-0.005029530730098486,
-0.05180229991674423,
-0.007601303514093161,
0.06285127252340317,
-0.07000945508480072,
-0.027768295258283615,
-0.050352174788713455,
-0.01157991774380207,
0.007237258367240429,
-0.06936395168304443,
0.026111070066690445,
0.04002882167696953,
-0.031201662495732307,
0.021835103631019592,
-0.04452672228217125,
0.02031015418469906,
-0.035877443850040436,
0.029362529516220093,
0.04647074639797211,
0.013716250658035278,
-0.012695766985416412,
0.01393605675548315,
0.07671459019184113,
-0.008806365542113781
] |
PMID:8212 | Glutamine-stimulated modification and degradation of glutamine synthetase in hepatoma tissue culture cells. | Effects of glutamine on glutamine synthetase (GS) activity of hepatoma tissue culture (HTC) cells were studied with the aid of a specific goat anti-rat GS serum. Immunodiffusion and immunoelectrophoretic tests show that rat liver GS and HTC cell GS are immunologically similar but not identical. Immunotitrations of HTC cell extracts demonstrate that in cells incubated in high concentrations (5 mM) of glutamine, a cross-reacting form of GS with a decreased enzyme-specific activity accumulates. On prolonged incubation of cells in high glutamine, there is net degradation of GS to form immunologically inactive products. Radio-immunoprecipitation experiments show that glutamine acts by accelerating the degradation of preformed GS. | Glutamine-stimulated modification and degradation of glutamine synthetase in hepatoma tissue culture cells. Effects of glutamine on glutamine synthetase (GS) activity of hepatoma tissue culture (HTC) cells were studied with the aid of a specific goat anti-rat GS serum. Immunodiffusion and immunoelectrophoretic tests show that rat liver GS and HTC cell GS are immunologically similar but not identical. Immunotitrations of HTC cell extracts demonstrate that in cells incubated in high concentrations (5 mM) of glutamine, a cross-reacting form of GS with a decreased enzyme-specific activity accumulates. On prolonged incubation of cells in high glutamine, there is net degradation of GS to form immunologically inactive products. Radio-immunoprecipitation experiments show that glutamine acts by accelerating the degradation of preformed GS. | [
0.017078429460525513,
-0.00963938795030117,
0.016190940514206886,
-0.011017916724085808,
0.042471449822187424,
-0.007266175001859665,
0.052943699061870575,
0.014650518074631691,
-0.07639548927545547,
0.0019387250067666173,
0.006212672684341669,
-0.055862560868263245,
0.0430140383541584,
0.02575242891907692,
-0.07920802384614944,
-0.05935995280742645,
0.00393067067489028,
-0.00009323147969553247,
-0.07772129774093628,
-0.055209118872880936,
-0.050722409039735794,
-0.008122237399220467,
0.052397340536117554,
-0.009853075258433819,
0.07527250051498413,
-0.007904368452727795,
-0.006933153141289949,
0.01994311809539795,
-0.05991138145327568,
-0.17896997928619385,
0.03278304636478424,
0.038322824984788895,
0.009693176485598087,
0.012876670807600021,
-0.019770149141550064,
0.03891855478286743,
-0.012773354537785053,
0.03942788019776344,
-0.004039350897073746,
0.032529566437006,
-0.011843601241707802,
0.0021525921765714884,
-0.02314787544310093,
0.03344183415174484,
0.04984283819794655,
-0.009265134111046791,
-0.010088183917105198,
-0.004403545055538416,
0.05398838222026825,
0.01554135326296091,
0.0533154122531414,
-0.04171593487262726,
0.040926966816186905,
0.01083762850612402,
0.014467018656432629,
-0.00038081989623606205,
-0.014436333440244198,
0.11617662757635117,
-0.025065073743462563,
-0.0038008817937225103,
-0.041194263845682144,
0.009843334555625916,
-0.11677476763725281,
0.04925671964883804,
0.08666984736919403,
-0.02669679746031761,
-0.04053570330142975,
-0.043762944638729095,
0.04266968369483948,
0.058875735849142075,
-0.08031866699457169,
0.006365302484482527,
-0.02477397583425045,
-0.011638417840003967,
0.005722378380596638,
0.0224961768835783,
0.006394246127456427,
0.03455271199345589,
0.03848198428750038,
0.029387546703219414,
0.044620007276535034,
-0.030822724103927612,
0.02357860468327999,
0.022096386179327965,
-0.022921981289982796,
-0.09446879476308823,
-0.04534337669610977,
0.002907716203480959,
0.01047324389219284,
-0.005028280895203352,
-0.06211410462856293,
-0.0717649906873703,
0.02703969180583954,
0.008077774196863174,
-0.09649658203125,
0.0024732768069952726,
-0.0003252585884183645,
0.002705118153244257,
-0.0076750791631639,
0.39162716269493103,
0.007041516713798046,
-0.035594601184129715,
-0.09337742626667023,
0.017849909141659737,
-0.009822970256209373,
-0.007258299738168716,
-0.04909767210483551,
0.03632683306932449,
0.0035852668806910515,
0.04398731142282486,
0.09699714928865433,
0.051490668207407,
-0.0010310563957318664,
0.004965377971529961,
-0.057892411947250366,
-0.01284057553857565,
0.07368328422307968,
0.02932100184261799,
-0.04901080206036568,
0.04024159535765648,
0.008140532299876213,
-0.007265449035912752,
0.005568682216107845,
-0.05756527557969093,
0.06727416813373566,
0.015879694372415543,
-0.06112087517976761,
0.08086152374744415,
0.033781662583351135,
-0.02218678779900074,
0.05080932751297951,
0.046357717365026474,
-0.032911982387304306,
0.011432148516178131,
0.015566636808216572,
0.008981103077530861,
0.01887865923345089,
-0.0033877016976475716,
0.05139652267098427,
-0.0011808975832536817,
0.012174932286143303,
-0.05384407564997673,
-0.055225394666194916,
-0.0545070543885231,
-0.07612918317317963,
0.10737547278404236,
0.004418537952005863,
-0.017288217321038246,
0.013535629026591778,
0.008793123066425323,
-0.01602722518146038,
0.040422677993774414,
0.05856899544596672,
0.026148203760385513,
-0.01708085462450981,
0.006384721491485834,
-0.01853008195757866,
-0.003526551416143775,
-0.019503548741340637,
-0.03393658623099327,
-0.0392276868224144,
-0.014434270560741425,
-0.03894076868891716,
0.06718115508556366,
0.049409568309783936,
-0.008175057359039783,
-0.03235838934779167,
0.00683711189776659,
-0.0018264850368723273,
0.02407500520348549,
-0.013345235027372837,
0.09142088890075684,
-0.0022818371653556824,
-0.03655882552266121,
-0.009986411780118942,
-0.02955893613398075,
0.03405378386378288,
0.00233911769464612,
0.04717981442809105,
-0.005491197109222412,
0.04020310565829277,
-0.006927373819053173,
-0.05111636593937874,
0.048384662717580795,
0.03708105534315109,
-0.03145657852292061,
-0.00919545441865921,
0.03680403530597687,
0.05268550664186478,
0.03259941563010216,
-0.04414932802319527,
0.004326874855905771,
-0.10942233353853226,
-0.0000897482896107249,
0.023039909079670906,
-0.06913250684738159,
-0.04868270829319954,
0.010717838071286678,
0.01868932694196701,
-0.05470982566475868,
0.07371850311756134,
0.05563532933592796,
0.015237852931022644,
0.05238356441259384,
-0.007317840587347746,
0.029127994552254677,
-0.002013768767938018,
0.004834605846554041,
0.048264872282743454,
-0.014960688538849354,
-0.06893198937177658,
0.01717706024646759,
-0.015205892734229565,
0.010273889638483524,
-0.014505652710795403,
0.040041133761405945,
-0.0011726038064807653,
-0.0025261228438466787,
0.047919612377882004,
0.008144904859364033,
0.045284777879714966,
-0.05808427557349205,
-0.016553346067667007,
-0.2960794270038605,
-0.0728021040558815,
0.014455471187829971,
-0.04450810328125954,
-0.008006885647773743,
-0.026252541691064835,
0.012705318629741669,
-0.051058538258075714,
0.038602057844400406,
-0.03777425363659859,
0.03763587027788162,
0.08843599259853363,
-0.009424647316336632,
-0.07572819292545319,
0.0626216009259224,
0.04016665741801262,
-0.00004253250881447457,
0.018401334062218666,
0.09541033953428268,
-0.012647051364183426,
0.06505800783634186,
-0.01420456264168024,
0.018992692232131958,
-0.0038148495368659496,
0.0778551995754242,
-0.028330883011221886,
0.10876377671957016,
0.026570674031972885,
-0.0020461012609302998,
0.0021205279044806957,
0.007566608022898436,
0.022174013778567314,
-0.061646606773138046,
-0.05276728421449661,
0.024666817858815193,
0.019740046933293343,
-0.03378807753324509,
-0.014174846932291985,
0.0012051439844071865,
0.022502711042761803,
-0.03632006794214249,
-0.04754985496401787,
-0.013302416540682316,
-0.1214352622628212,
-0.01277046836912632,
0.011124208569526672,
0.014795096591114998,
-0.016188690438866615,
0.006407276727259159,
-0.06568273901939392,
0.046230435371398926,
-0.028173526749014854,
-0.022087274119257927,
0.037025731056928635,
0.009701581671833992,
0.035033177584409714,
-0.018001314252614975,
0.04787297919392586,
-0.10076387971639633,
0.0341898649930954,
-0.014393740333616734,
0.048636533319950104,
-0.028850330039858818,
0.016483591869473457,
-0.03977873548865318,
0.028415285050868988,
0.027041731402277946,
0.003865361912176013,
-0.02028016559779644,
0.01654234528541565,
0.06825871020555496,
0.08061818778514862,
-0.022680116817355156,
0.06081999093294144,
0.014343734830617905,
-0.025063298642635345,
-0.035013772547245026,
-0.0502132773399353,
-0.1065361350774765,
0.0189245343208313,
-0.04656057059764862,
0.012349833734333515,
0.0570359006524086,
0.0030366675928235054,
0.02589997835457325,
0.048885688185691833,
0.024611560627818108,
-0.038298193365335464,
0.023485474288463593,
-0.003769000293686986,
0.024989457800984383,
0.011089066974818707,
-0.05901024863123894,
-0.07511146366596222,
-0.011399959214031696,
-0.02116469293832779,
-0.21953949332237244,
0.004073165822774172,
0.004424151033163071,
-0.015593418851494789,
-0.08478643745183945,
-0.019665518775582314,
-0.011904733255505562,
-0.005080036353319883,
-0.04319056123495102,
0.059740304946899414,
0.05617684870958328,
0.004531274549663067,
0.08088856190443039,
0.0178145170211792,
-0.003645922988653183,
0.0023368902038782835,
0.02592175453901291,
-0.036036305129528046,
0.01114396471530199,
-0.049121756106615067,
0.03904486075043678,
-0.04758505895733833,
0.11937858909368515,
-0.015520469285547733,
0.015223200432956219,
0.04228353872895241,
-0.013023793697357178,
0.016957111656665802,
0.009702606126666069,
0.029995813965797424,
-0.03223458677530289,
0.027457116171717644,
0.11447005718946457,
-0.02595675177872181,
0.0584285669028759,
0.03294717147946358,
-0.037844836711883545,
0.00480658421292901,
-0.09701752662658691,
0.044422782957553864,
-0.010091491043567657,
0.022148393094539642,
-0.03826970234513283,
-0.038753289729356766,
0.07752618193626404,
-0.04112366586923599,
-0.04010739549994469,
-0.07258450239896774,
-0.010563686490058899,
-0.035734497010707855,
-0.026696277782320976,
-0.0417507030069828,
0.03299466520547867,
0.0016430048272013664,
-0.015773188322782516,
-0.03714375197887421,
-0.04356005787849426,
-0.004967620596289635,
0.010533519089221954,
0.01944156363606453,
-0.01897403784096241,
0.019243141636252403,
-0.035454921424388885,
0.11230532824993134,
-0.020321495831012726
] |
PMID:8213 | Membrane receptors for murine leukemia viruses: characterization using the purified viral envelope glycoprotein, gp71. | The 71,000 dalton glycoprotein (gp71) purified from Rauscher murine leukemia virus (R-MuLV) by affinity chromatography specifically binds to murine but not other mammalian cells in culture. Binding is prevented by specific antiserum raides to gp71 (anti-gp71). The binding assay as described in this report can detect receptors on as few as 300 murine cells, and with 1 X 10(5) cells gives significant binding with 30 sec. The results show that the purified glycoprotein retians biologic activity and can form a stable complex with specific receptors on mouse cell membranes. The assay can therefore be used to characterize the nature of the cellular receptors that are essential for leukemia virus infection. Purified gp71 binding to mouse cells is prevented if the cells are actively producing related ecotropic type C viruses, presumably because the receptors are occupied and are not available to bind exogenously applied gp71. The binding of gp71 to murine cells is enhanced by the presence of calcium ions and low pH. Binding studies performed using an excess of 125I-gp71 indicate the NIH/3T3 cells bind approximately 5.3 X 10(5) molecules of 125I-gp71 per cell. | Membrane receptors for murine leukemia viruses: characterization using the purified viral envelope glycoprotein, gp71. The 71,000 dalton glycoprotein (gp71) purified from Rauscher murine leukemia virus (R-MuLV) by affinity chromatography specifically binds to murine but not other mammalian cells in culture. Binding is prevented by specific antiserum raides to gp71 (anti-gp71). The binding assay as described in this report can detect receptors on as few as 300 murine cells, and with 1 X 10(5) cells gives significant binding with 30 sec. The results show that the purified glycoprotein retians biologic activity and can form a stable complex with specific receptors on mouse cell membranes. The assay can therefore be used to characterize the nature of the cellular receptors that are essential for leukemia virus infection. Purified gp71 binding to mouse cells is prevented if the cells are actively producing related ecotropic type C viruses, presumably because the receptors are occupied and are not available to bind exogenously applied gp71. The binding of gp71 to murine cells is enhanced by the presence of calcium ions and low pH. Binding studies performed using an excess of 125I-gp71 indicate the NIH/3T3 cells bind approximately 5.3 X 10(5) molecules of 125I-gp71 per cell. | [
-0.05675409361720085,
-0.012233010493218899,
-0.03992202877998352,
0.004465857055038214,
-0.038698166608810425,
-0.01296741608530283,
0.07545585930347443,
0.037463750690221786,
-0.043352000415325165,
-0.01589089259505272,
0.02728314884006977,
-0.09261421859264374,
0.006984441541135311,
0.009350825101137161,
-0.05682377144694328,
-0.05308198556303978,
0.024911394342780113,
-0.008129735477268696,
-0.0432470366358757,
0.025779759511351585,
-0.05618714168667793,
-0.11004073917865753,
-0.06451130658388138,
-0.07016520202159882,
0.04089217260479927,
-0.006284871604293585,
-0.053842730820178986,
-0.031984321773052216,
-0.04133042320609093,
-0.18767252564430237,
-0.02986432984471321,
-0.00270627299323678,
0.030069943517446518,
0.006738709285855293,
-0.05439196899533272,
0.009415692649781704,
0.01140647567808628,
-0.049162186682224274,
-0.01436962652951479,
0.01691371202468872,
0.05858022719621658,
0.019034385681152344,
-0.013035949319601059,
0.016883768141269684,
0.051332470029592514,
-0.02709915302693844,
-0.05342110991477966,
0.00471952510997653,
0.07183659076690674,
-0.020235082134604454,
0.011610550805926323,
0.009580254554748535,
0.010578678920865059,
0.03177569806575775,
-0.06382256001234055,
-0.009028099477291107,
-0.00852509681135416,
0.031235646456480026,
0.00964692048728466,
0.08104266226291656,
-0.003840348683297634,
-0.03062831610441208,
-0.13446462154388428,
0.06749604642391205,
0.027112912386655807,
-0.05854655057191849,
-0.029413588345050812,
0.007359806448221207,
0.06005437672138214,
0.06220680847764015,
-0.05000677704811096,
0.02940160594880581,
-0.06515693664550781,
0.038521770387887955,
-0.04880567640066147,
-0.03936504200100899,
-0.006194028537720442,
0.00848025269806385,
0.053330522030591965,
0.05648084357380867,
-0.020370643585920334,
-0.030427750200033188,
0.023181850090622902,
-0.014978842809796333,
-0.060632023960351944,
0.008351938799023628,
-0.025883961468935013,
0.004465365782380104,
0.007644831668585539,
0.0321483351290226,
-0.04510139673948288,
-0.022595705464482307,
0.014037257060408592,
0.014216868206858635,
-0.10541719943284988,
0.017682891339063644,
0.0446757972240448,
-0.010252697393298149,
0.0017084459541365504,
0.3635478913784027,
-0.035355258733034134,
0.011682091280817986,
-0.0697498470544815,
0.06730647385120392,
0.026717795059084892,
-0.015580432489514351,
-0.04547325149178505,
0.0073767416179180145,
-0.04056716337800026,
0.07004188001155853,
0.02523924969136715,
-0.025707485154271126,
0.017361311241984367,
-0.029517794027924538,
0.03300680220127106,
0.00606551906093955,
0.03310873731970787,
-0.0004075652686879039,
-0.008547043427824974,
0.01902172714471817,
-0.023627694696187973,
-0.03969521075487137,
0.013920694589614868,
0.0030582286417484283,
0.12342267483472824,
0.039136309176683426,
-0.042884740978479385,
0.06741451472043991,
0.08865191042423248,
0.05259965732693672,
0.01890011876821518,
0.04939161241054535,
-0.0625305101275444,
0.04686049371957779,
0.06886713951826096,
-0.025558695197105408,
-0.002556669292971492,
-0.00906549021601677,
0.004945054184645414,
-0.0003398430999368429,
0.017051244154572487,
0.043498553335666656,
-0.023986704647541046,
-0.03951064497232437,
-0.031225435435771942,
0.056558866053819656,
-0.03296360746026039,
0.010811240412294865,
-0.03134825453162193,
0.002419681055471301,
0.030891720205545425,
0.09383969753980637,
-0.026154624298214912,
0.018910298123955727,
-0.0020439084619283676,
0.06057911366224289,
0.0053929053246974945,
0.018718810752034187,
-0.02262962982058525,
0.03727763146162033,
-0.04234012961387634,
0.04153366759419441,
0.02089974470436573,
0.03797110170125961,
0.031155407428741455,
0.001628643716685474,
-0.03990346938371658,
-0.03041829727590084,
0.02645181678235531,
0.04861420765519142,
-0.024265950545668602,
0.0073265996761620045,
0.003408600576221943,
0.015216120518743992,
-0.02268660068511963,
-0.021646294742822647,
-0.07767574489116669,
0.015521763823926449,
0.015340291894972324,
0.00011574066593311727,
-0.021176869049668312,
-0.03435390815138817,
-0.028285663574934006,
0.009228532202541828,
0.02543834038078785,
-0.029385840520262718,
-0.043341755867004395,
0.006203111261129379,
0.01471165381371975,
0.06057612597942352,
-0.02169550210237503,
0.022596117109060287,
-0.03618913143873215,
-0.03891816362738609,
-0.016145728528499603,
-0.03351205959916115,
0.010278617963194847,
-0.06788108497858047,
-0.06077756732702255,
-0.06442248076200485,
0.13174037635326385,
-0.01826007477939129,
-0.011336658149957657,
0.08735702931880951,
0.007775303442031145,
0.02791871502995491,
0.012083331122994423,
-0.013690759427845478,
0.0451962910592556,
-0.006601010449230671,
0.0027311043813824654,
0.026769734919071198,
-0.08501017838716507,
-0.03490445390343666,
-0.03528714179992676,
0.033034566789865494,
0.023506464436650276,
-0.026603354141116142,
0.06649570912122726,
-0.028602609410881996,
0.029774649068713188,
0.02692331373691559,
-0.0009235286852344871,
-0.294434517621994,
-0.017045440152287483,
-0.00039099628338590264,
-0.0032125688157975674,
-0.012269726023077965,
-0.06725254654884338,
0.044669970870018005,
-0.019551102072000504,
0.0029316844884306192,
-0.06163100525736809,
0.04896248131990433,
0.05635400116443634,
-0.03695929795503616,
-0.038520339876413345,
-0.022553736343979836,
0.07373358309268951,
-0.028153806924819946,
0.0271005742251873,
0.024632634595036507,
-0.03430365398526192,
-0.010088187642395496,
-0.006269458681344986,
-0.027230581268668175,
0.07472724467515945,
0.0474763922393322,
-0.05110163241624832,
0.1351962685585022,
0.08776310086250305,
-0.012844857759773731,
-0.0017007521819323301,
0.013069014064967632,
0.012430255301296711,
-0.037792425602674484,
-0.046062372624874115,
-0.03555512800812721,
0.006180998403578997,
-0.05322457104921341,
-0.00574733829125762,
0.03780556842684746,
-0.010670024901628494,
-0.01574603095650673,
0.00594663992524147,
-0.01095716655254364,
-0.045006584376096725,
-0.02412515878677368,
-0.008146846666932106,
0.010724368505179882,
0.07102594524621964,
-0.00937806535512209,
0.009530159644782543,
0.042138002812862396,
-0.03598226234316826,
0.029901018366217613,
-0.0134321553632617,
0.005530196707695723,
0.039908092468976974,
0.008654176257550716,
0.034981582313776016,
-0.004916276317089796,
0.039898332208395004,
-0.013149397447705269,
-0.01346430554986,
-0.011988713406026363,
0.033553510904312134,
0.005044981837272644,
-0.015391824766993523,
0.05646905303001404,
0.004445512779057026,
0.04316085949540138,
-0.014901624992489815,
-0.045272719115018845,
0.0819714143872261,
0.016337983310222626,
0.08640479296445847,
0.036998942494392395,
0.059263404458761215,
-0.007630873471498489,
-0.05920973792672157,
-0.09197646379470825,
0.01304836105555296,
-0.014659442938864231,
-0.01864057406783104,
0.033579789102077484,
-0.026896782219409943,
0.014830056577920914,
-0.009669814258813858,
0.02978518232703209,
0.0959794744849205,
0.039773136377334595,
0.03149650990962982,
-0.0020300119649618864,
0.0013911715941503644,
-0.02994384430348873,
-0.06301623582839966,
0.025772251188755035,
0.0003204378590453416,
-0.2765560448169708,
-0.014650383032858372,
0.035773713141679764,
0.03215715289115906,
-0.04469895362854004,
0.0038679707795381546,
0.0739147737622261,
-0.026187516748905182,
-0.060961365699768066,
-0.009650910273194313,
0.07192011922597885,
0.05640728399157524,
0.04660284146666527,
0.05657249689102173,
-0.00773554015904665,
-0.001311437925323844,
0.08396808058023453,
-0.08212293684482574,
0.0076103112660348415,
0.06442365050315857,
0.01585421897470951,
-0.0652761161327362,
0.14187918603420258,
0.0074577853083610535,
0.009708232246339321,
0.03037223406136036,
-0.08120567351579666,
-0.03252986818552017,
-0.013405288569629192,
-0.02024192176759243,
-0.020396390929818153,
-0.030797870829701424,
0.050318699330091476,
-0.03856769949197769,
-0.015540061518549919,
0.06913591921329498,
-0.0021574979182332754,
0.03378113731741905,
-0.04495291784405708,
0.02139028161764145,
0.020855819806456566,
0.07417058944702148,
-0.05318243056535721,
-0.058260586112737656,
0.08945796638727188,
-0.04690976440906525,
-0.03253103420138359,
-0.024855773895978928,
-0.018914848566055298,
0.014171959832310677,
-0.05692354217171669,
-0.0017986921593546867,
-0.02587854117155075,
-0.007949386723339558,
0.01542588509619236,
-0.030752141028642654,
0.02711903676390648,
0.0033553794492036104,
-0.012694720178842545,
0.007303581107407808,
-0.006554931867867708,
0.006309308111667633,
0.0492633692920208,
0.05549324303865433,
0.038561757653951645
] |
PMID:8214 | Naturally occurring cross-links in yeast chromosomal DNA. | Chromosome-size yeast DNA molecules with a number average molecular weight (Mn) of 3-4 X 10(8) were isolated from sucrose gradients after sedimentation of lysed yeast spheroplasts. Resedimentation showed that the molecules were isolated without introducing appreciable single-strand or double-strand breaks. The presence of cross-links in these molecules was suggested by the observation that the apparent Mn in alkali was greater than expected for separated single strands. Since cross-linked molecules would have strands which fail to separate upon denaturation, this was tested more directly. Neutralization of alkaline denaturing conditions resulted in up to 70% of the intact molecules rapidly reforming duplex structures, as shown by equilibrium banding in CsCI. Experiments with larger E. coli DNA molecules (Mn = 5.2 X 10(8)) indicated that the conditions used were sufficient to denature completely molecules of this size. Results of enzyme treatments suggest that the cross-links are not RNA or protein. Experiments with density-labeled yeast DNA molecules showed that the rapid reformation of duplex DNA is not the consequence either of a bimolecular reaction between separated DNA strands or of intrastrand renaturation. The data indicate that when the yeast DNA molecules are completely denatured, the strands fail to separate. Hence they must be cross-linked. Experiments with sheared DNA show that there are small number of cross-links, one to four, permolecule. | Naturally occurring cross-links in yeast chromosomal DNA. Chromosome-size yeast DNA molecules with a number average molecular weight (Mn) of 3-4 X 10(8) were isolated from sucrose gradients after sedimentation of lysed yeast spheroplasts. Resedimentation showed that the molecules were isolated without introducing appreciable single-strand or double-strand breaks. The presence of cross-links in these molecules was suggested by the observation that the apparent Mn in alkali was greater than expected for separated single strands. Since cross-linked molecules would have strands which fail to separate upon denaturation, this was tested more directly. Neutralization of alkaline denaturing conditions resulted in up to 70% of the intact molecules rapidly reforming duplex structures, as shown by equilibrium banding in CsCI. Experiments with larger E. coli DNA molecules (Mn = 5.2 X 10(8)) indicated that the conditions used were sufficient to denature completely molecules of this size. Results of enzyme treatments suggest that the cross-links are not RNA or protein. Experiments with density-labeled yeast DNA molecules showed that the rapid reformation of duplex DNA is not the consequence either of a bimolecular reaction between separated DNA strands or of intrastrand renaturation. The data indicate that when the yeast DNA molecules are completely denatured, the strands fail to separate. Hence they must be cross-linked. Experiments with sheared DNA show that there are small number of cross-links, one to four, permolecule. | [
-0.07421224564313889,
-0.018983114510774612,
-0.007009176071733236,
-0.0015275974292308092,
0.02687232568860054,
-0.03437158092856407,
-0.0010534939356148243,
0.04470498487353325,
0.010081194341182709,
0.03509124368429184,
0.034811876714229584,
-0.04495101049542427,
0.03564799949526787,
0.010618320666253567,
-0.0377456396818161,
-0.021677536889910698,
-0.04856519028544426,
0.03165382519364357,
-0.034595418721437454,
0.037938833236694336,
0.021669460460543633,
-0.017216647043824196,
0.021252388134598732,
-0.036957040429115295,
0.007643659133464098,
0.0407928004860878,
-0.014711488038301468,
-0.029157735407352448,
-0.02652369625866413,
-0.20931720733642578,
0.04753594473004341,
-0.017365988343954086,
-0.020700965076684952,
-0.013849962502717972,
0.00790335237979889,
-0.016942007467150688,
-0.02093534730374813,
-0.04855030030012131,
-0.03894558176398277,
0.0072522591799497604,
0.1132073923945427,
0.020743144676089287,
0.027450939640402794,
0.029585953801870346,
-0.036235153675079346,
0.009617268107831478,
-0.029489383101463318,
0.004670554772019386,
0.025838268920779228,
-0.011189597658813,
-0.04050270840525627,
0.026337070390582085,
-0.00489445123821497,
0.013818426057696342,
-0.061984214931726456,
0.06798001378774643,
0.06589395552873611,
0.03061423823237419,
0.002759257098659873,
0.02107013389468193,
-0.016735315322875977,
0.020548783242702484,
-0.06383687257766724,
0.027381040155887604,
0.04759714752435684,
0.04780597612261772,
-0.007252648007124662,
-0.07566026598215103,
0.01284018624573946,
0.07195889949798584,
-0.02778293378651142,
0.01743030920624733,
0.028252331539988518,
0.1019202247262001,
0.060997579246759415,
-0.0054916925728321075,
0.0008027763688005507,
-0.0266608614474535,
0.04175325110554695,
-0.03345465660095215,
-0.01707906648516655,
-0.02797028422355652,
0.03992972895503044,
-0.035498473793268204,
-0.05899818241596222,
0.06906381249427795,
0.009860615245997906,
-0.028805315494537354,
-0.003581524593755603,
-0.043671853840351105,
0.008159399032592773,
-0.013746656477451324,
0.037074435502290726,
0.03406454995274544,
-0.07170455902814865,
-0.006421671248972416,
0.04304422810673714,
0.03048878163099289,
0.06460821628570557,
0.3598306179046631,
-0.05215480923652649,
0.014411012642085552,
0.00900089368224144,
-0.01440791692584753,
-0.055426545441150665,
-0.04856335371732712,
-0.00995652936398983,
-0.0038413447327911854,
0.06933294236660004,
0.019649608060717583,
0.04807485267519951,
-0.008799184113740921,
0.006253763101994991,
-0.046304501593112946,
0.03090793825685978,
0.013657469302415848,
0.017531977966427803,
-0.0006641880027018487,
-0.059863727539777756,
0.011644377373158932,
-0.07301436364650726,
0.020033806562423706,
-0.02110425941646099,
-0.0023834756575524807,
0.0034420806914567947,
0.03697957843542099,
-0.02838555909693241,
0.06727531552314758,
0.06082852557301521,
-0.005866562947630882,
0.018191343173384666,
-0.007728173863142729,
-0.021595925092697144,
-0.0025247999001294374,
-0.0804942175745964,
-0.04198906570672989,
-0.008328532800078392,
-0.05316585674881935,
0.03493385389447212,
-0.012475617229938507,
-0.00004526841803453863,
-0.07405643910169601,
-0.026755839586257935,
-0.05072898790240288,
-0.05370776727795601,
0.06489522755146027,
-0.022205475717782974,
0.09541361778974533,
-0.006297972518950701,
0.03523923084139824,
0.015845391899347305,
0.08833165466785431,
0.001397917978465557,
0.0009259600774385035,
-0.019278014078736305,
0.005031795706599951,
-0.03323247283697128,
-0.025260133668780327,
0.0025446543004363775,
0.05419211462140083,
-0.034358520060777664,
0.03540559485554695,
0.02089153788983822,
0.12614910304546356,
0.010845995508134365,
-0.08356491476297379,
0.03164604678750038,
0.01768799126148224,
0.018158938735723495,
-0.043952856212854385,
0.012698879465460777,
-0.045598264783620834,
-0.016827818006277084,
0.00353801972232759,
-0.03613097593188286,
-0.024458762258291245,
-0.07975997775793076,
-0.02302180975675583,
0.013960192911326885,
0.0009142514318227768,
0.06237077713012695,
-0.027991915121674538,
0.015158395282924175,
0.04510614275932312,
0.011761670000851154,
-0.01127347256988287,
-0.0998459905385971,
0.003718386637046933,
0.006635262630879879,
0.06597542762756348,
-0.021931275725364685,
0.010885459370911121,
-0.06710720807313919,
0.0535261332988739,
-0.018219009041786194,
0.021629249677062035,
-0.008816448971629143,
-0.012397881597280502,
-0.04393942654132843,
0.04632015526294708,
0.07406742125749588,
0.038988083600997925,
0.021154893562197685,
0.02004549652338028,
-0.08462454378604889,
-0.029198559001088142,
0.04257790371775627,
-0.03989275544881821,
0.01255832239985466,
-0.015626834705471992,
-0.021533260121941566,
-0.03949609026312828,
-0.08198515325784683,
-0.033652160316705704,
-0.03632882609963417,
-0.008795831352472305,
0.014406057074666023,
0.023113958537578583,
0.05361820012331009,
0.006444149184972048,
0.039210621267557144,
-0.08243317157030106,
-0.042198047041893005,
-0.31446853280067444,
0.01982986368238926,
-0.0518973246216774,
0.05604057386517525,
0.07524845749139786,
-0.039947327226400375,
0.0043576681055128574,
-0.012640750035643578,
0.006965679582208395,
-0.04616156220436096,
0.0701962560415268,
0.06472782790660858,
-0.06716080754995346,
-0.12615418434143066,
0.001089685596525669,
0.05103219300508499,
0.03820602595806122,
-0.023360101506114006,
0.0007424820796586573,
0.031170478090643883,
0.021448615938425064,
0.03593000769615173,
-0.014619487337768078,
0.01089521124958992,
0.04867657274007797,
-0.03647235035896301,
0.11816996335983276,
-0.030807433649897575,
0.021403880789875984,
0.020736992359161377,
-0.0005722037749364972,
0.03543875366449356,
-0.031563058495521545,
-0.03335496783256531,
-0.010708536952733994,
-0.017664089798927307,
-0.0058210003189742565,
-0.06084991246461868,
0.01134722400456667,
0.02987545356154442,
-0.04867621138691902,
-0.016951950266957283,
-0.03367944434285164,
-0.03485950082540512,
0.03699684143066406,
-0.005017475225031376,
0.0036835239734500647,
-0.03941088542342186,
0.07480183988809586,
-0.005740465596318245,
0.03691582381725311,
-0.02261831797659397,
0.036682359874248505,
0.01904449239373207,
0.024086175486445427,
0.02857021987438202,
-0.05709461495280266,
-0.022725403308868408,
-0.04909231886267662,
-0.04268971085548401,
0.050875674933195114,
0.0005505742155946791,
-0.03751904144883156,
0.03300796449184418,
0.0036758584901690483,
-0.021176589652895927,
-0.012655054219067097,
0.013022925704717636,
0.04837896302342415,
-0.0021218229085206985,
0.000338830694090575,
0.11387846618890762,
0.013985374942421913,
0.02383420802652836,
0.020843563601374626,
0.05281827598810196,
-0.0693913996219635,
-0.01940951496362686,
-0.06549643725156784,
0.02569172903895378,
0.04289906471967697,
-0.03297574818134308,
0.0561722107231617,
-0.013395560905337334,
-0.0055971527472138405,
0.032046277076005936,
0.02307918295264244,
-0.00887219700962305,
0.04304119944572449,
-0.005891226232051849,
0.0237010195851326,
-0.013173794373869896,
0.03884518891572952,
-0.023698585107922554,
0.036109454929828644,
-0.0010485945967957377,
-0.2877783477306366,
0.04650353640317917,
0.03114086389541626,
0.016496168449521065,
-0.004791324958205223,
0.0479012131690979,
0.10425196588039398,
-0.03202877938747406,
-0.016200393438339233,
-0.013822306878864765,
0.007127869408577681,
0.029271669685840607,
0.043574243783950806,
0.007944177836179733,
0.010387635789811611,
0.06737636029720306,
0.03426311910152435,
-0.038917023688554764,
0.0004497457994148135,
0.039663590490818024,
0.020058192312717438,
-0.07034125924110413,
0.1556088924407959,
0.019491713494062424,
-0.00547690037637949,
0.0370279997587204,
-0.03354427590966225,
0.04697104170918465,
-0.018089765682816505,
-0.011367231607437134,
-0.03596620634198189,
0.04132697731256485,
0.0342848114669323,
-0.05095771700143814,
0.0468478724360466,
0.02563467249274254,
0.013898990117013454,
-0.0600188784301281,
0.041015781462192535,
-0.04269438609480858,
-0.037815459072589874,
-0.027174165472388268,
-0.08358567953109741,
-0.049382343888282776,
0.03607366234064102,
-0.05637028068304062,
-0.05530533567070961,
-0.04386746138334274,
-0.009955122135579586,
-0.011483686976134777,
-0.052886705845594406,
-0.026644758880138397,
0.026117786765098572,
0.020632505416870117,
-0.014243412762880325,
-0.030062885954976082,
-0.04927720129489899,
-0.0027754774782806635,
0.042127009481191635,
0.022924188524484634,
0.04754551500082016,
-0.0109806964173913,
-0.06785441935062408,
0.10143757611513138,
-0.009846710599958897
] |
PMID:8216 | The effect of allyl compounds on hepatic microsomal mixed function oxidation and porphyrogenesis. | The activities of 5-aminolaevulinate (5-ALA) synthetase and of various microsomat drug-metabolising enzymes have been determined in the livers of rats pretreated with different drugs and chemicals containing the allyl group. Safrole, isosafrole and secobarbital gave rise to slight increases in 5-ALA synthetase, whereas alclophenac and triallyl cyanurate almost doubled the enzyme activity and the known porphyrogenic agents, allylisopropylacetamide (AIA) and allobarbital caused increases of 1.5- and 2.5-fold, respectively. Allobarbital induced the microsomal drug-metabolising enzymes while secobarbital had only a weak effect and alclophenac and triallyl cyanurate had no effect at all. From these results it is suggested that induction of the synthesis of cytochrome P-450 is not rate dependent on the synthesis haem and induction of porphyrin biosynthesis. | The effect of allyl compounds on hepatic microsomal mixed function oxidation and porphyrogenesis. The activities of 5-aminolaevulinate (5-ALA) synthetase and of various microsomat drug-metabolising enzymes have been determined in the livers of rats pretreated with different drugs and chemicals containing the allyl group. Safrole, isosafrole and secobarbital gave rise to slight increases in 5-ALA synthetase, whereas alclophenac and triallyl cyanurate almost doubled the enzyme activity and the known porphyrogenic agents, allylisopropylacetamide (AIA) and allobarbital caused increases of 1.5- and 2.5-fold, respectively. Allobarbital induced the microsomal drug-metabolising enzymes while secobarbital had only a weak effect and alclophenac and triallyl cyanurate had no effect at all. From these results it is suggested that induction of the synthesis of cytochrome P-450 is not rate dependent on the synthesis haem and induction of porphyrin biosynthesis. | [
-0.05014244467020035,
0.010807393118739128,
-0.012160440906882286,
0.029647167772054672,
-0.0029464031103998423,
-0.033543407917022705,
0.04024234414100647,
-0.024569744244217873,
-0.07560541480779648,
0.0034456064458936453,
0.022886348888278008,
-0.01725955866277218,
-0.007803380489349365,
0.0448264479637146,
0.021930797025561333,
-0.007328190375119448,
-0.003268032567575574,
-0.009770783595740795,
-0.039286572486162186,
-0.04517066478729248,
-0.07107235491275787,
0.007677966728806496,
0.10194873064756393,
0.005048662889748812,
0.031486667692661285,
0.0453253835439682,
0.023470256477594376,
-0.01222224161028862,
-0.07613618671894073,
-0.18866311013698578,
0.06292997300624847,
0.045852236449718475,
0.01497422344982624,
-0.05369044840335846,
0.004883372224867344,
0.009327047504484653,
-0.0701805129647255,
0.03495132178068161,
0.008714619092643261,
0.03270445391535759,
0.013140060938894749,
0.013307344168424606,
-0.0006388473557308316,
0.006305238697677851,
-0.00778175750747323,
0.009867798537015915,
0.04727580398321152,
0.03180961310863495,
0.055542122572660446,
0.04461618885397911,
0.031364258378744125,
-0.039314962923526764,
0.0037196052726358175,
0.0649934932589531,
-0.002051042625680566,
-0.004271895624697208,
0.02041376754641533,
0.06405112892389297,
-0.016174232587218285,
0.06024552509188652,
-0.030614428222179413,
0.05242284759879112,
-0.16927878558635712,
0.05464908853173256,
0.03561783209443092,
0.012155403383076191,
0.003197813406586647,
-0.051527488976716995,
0.08214458078145981,
0.05002795532345772,
-0.07437166571617126,
-0.014763535000383854,
-0.00046355914673767984,
0.02169111743569374,
0.012567108497023582,
0.027890125289559364,
0.007363708224147558,
-0.05996594950556755,
0.017105484381318092,
0.0035548945888876915,
0.021479563787579536,
-0.0031633502803742886,
-0.03158232197165489,
0.04196329042315483,
0.041503261774778366,
-0.045762352645397186,
-0.013477464206516743,
-0.022869328036904335,
0.008737320080399513,
0.029214011505246162,
-0.03553473949432373,
-0.06290276348590851,
0.013141180388629436,
-0.06401459872722626,
-0.021696243435144424,
-0.02383285202085972,
0.005844200029969215,
-0.012021609582006931,
-0.022732924669981003,
0.3801881968975067,
-0.0029129718896001577,
0.03398147225379944,
-0.06440186500549316,
0.010722072795033455,
0.03168540075421333,
-0.030790340155363083,
0.02279478870332241,
0.04443254694342613,
-0.009938155300915241,
0.006792398635298014,
0.10366662591695786,
-0.003810521448031068,
0.07141504436731339,
-0.017728455364704132,
-0.06170865148305893,
-0.01704876311123371,
0.07831820100545883,
-0.028097646310925484,
-0.05383811891078949,
0.0618831031024456,
-0.026406580582261086,
-0.029672035947442055,
0.0571369007229805,
0.04839353263378143,
0.03416862338781357,
0.014272227883338928,
-0.029096487909555435,
0.058479923754930496,
0.0879359245300293,
0.039294224232435226,
0.06449434161186218,
0.030715318396687508,
0.012730893678963184,
-0.024342332035303116,
-0.029949137941002846,
-0.054192300885915756,
0.02998073771595955,
-0.04931459575891495,
0.06855212152004242,
0.02224743925035,
-0.05766081064939499,
-0.04090731963515282,
0.008395053446292877,
-0.038038574159145355,
-0.042187418788671494,
0.11891621351242065,
0.07136989384889603,
0.040412768721580505,
-0.0010850614635273814,
0.027871696278452873,
-0.00475169625133276,
0.012660900130867958,
-0.05381118133664131,
0.0848214328289032,
-0.04123172536492348,
0.061408668756484985,
-0.041492704302072525,
-0.02528691105544567,
0.011855152435600758,
0.03227055445313454,
-0.12141269445419312,
-0.02793373540043831,
-0.0271372701972723,
0.07692687213420868,
-0.006932354532182217,
-0.035999201238155365,
-0.03794190287590027,
0.028161756694316864,
-0.047811586409807205,
-0.015428642742335796,
0.012566676363348961,
0.02546875923871994,
-0.014295370317995548,
-0.03189757466316223,
-0.025957822799682617,
0.023398708552122116,
0.06528323888778687,
-0.003081158734858036,
0.051856838166713715,
0.010459855198860168,
0.06149746850132942,
0.030889932066202164,
-0.043478481471538544,
0.0429236963391304,
-0.05067507177591324,
-0.04812334477901459,
-0.0664878860116005,
-0.031121376901865005,
0.026662567630410194,
0.03247735649347305,
-0.010267061181366444,
0.053931817412376404,
-0.039126403629779816,
0.019119620323181152,
-0.05231580138206482,
-0.03894961252808571,
-0.05144672095775604,
0.01972089149057865,
0.004253630060702562,
-0.06894471496343613,
0.0831979513168335,
0.014823482371866703,
0.05553280934691429,
0.0833912193775177,
0.028979819267988205,
0.036587439477443695,
-0.0151929697021842,
-0.00765480799600482,
0.07317843288183212,
-0.035383790731430054,
-0.025180634111166,
-0.006635064259171486,
-0.02968313731253147,
0.06675166636705399,
-0.04345996305346489,
0.03440030664205551,
-0.03960483893752098,
0.008774018846452236,
0.03585256263613701,
-0.004590010270476341,
0.06578683108091354,
-0.07072112709283829,
-0.03698474541306496,
-0.27456134557724,
-0.021137047559022903,
-0.03260274603962898,
-0.05424550548195839,
-0.008150528185069561,
-0.045397136360406876,
-0.005642779171466827,
-0.020143568515777588,
-0.03271573781967163,
0.008688339963555336,
0.035695817321538925,
0.050626713782548904,
0.011918166652321815,
-0.03041740134358406,
-0.019995497539639473,
0.02176652103662491,
0.017773684114217758,
0.004501553252339363,
0.044168777763843536,
-0.005712683312594891,
-0.0142572782933712,
-0.05364197492599487,
-0.005836451426148415,
-0.016563886776566505,
0.07241995632648468,
-0.00623099273070693,
0.08869114518165588,
0.03096424974501133,
-0.03135398030281067,
-0.053110044449567795,
0.02154035121202469,
0.06325384229421616,
-0.023420505225658417,
-0.09952963888645172,
-0.009136222302913666,
-0.03852942958474159,
0.0229734405875206,
-0.07298124581575394,
0.01845146343111992,
-0.01128246821463108,
-0.028979381546378136,
0.03229209780693054,
-0.04792182892560959,
-0.06489651650190353,
-0.0399775505065918,
0.005063365679234266,
-0.02970062382519245,
0.017124755308032036,
-0.00991396140307188,
-0.03502182289958,
-0.006448865868151188,
0.04942959547042847,
0.055703237652778625,
0.007885209284722805,
-0.0008993776282295585,
-0.014604086987674236,
-0.04968733340501785,
-0.03215499594807625,
-0.04835858941078186,
-0.015251165255904198,
0.004673651419579983,
-0.003169330535456538,
0.03246027231216431,
0.02692197449505329,
-0.031315043568611145,
-0.01132682804018259,
-0.05361005663871765,
-0.016777178272604942,
0.04726031795144081,
0.05997238680720329,
0.016370980069041252,
0.10160623490810394,
-0.0409599244594574,
0.005889305379241705,
0.04793355613946915,
0.0012167885433882475,
-0.023011377081274986,
-0.05731358751654625,
-0.06869542598724365,
0.036388132721185684,
-0.028252974152565002,
-0.014490091241896152,
0.0336884930729866,
0.01889192685484886,
-0.030942747369408607,
0.000017192260202136822,
-0.005433769430965185,
-0.007170711178332567,
0.028447354212403297,
0.023207364603877068,
0.035798441618680954,
-0.03311649709939957,
-0.028580784797668457,
-0.0171259343624115,
0.05541623756289482,
-0.012484295293688774,
-0.25294068455696106,
-0.03130444884300232,
0.013135290704667568,
-0.03662784397602081,
-0.05320983752608299,
-0.013419874012470245,
0.04955609515309334,
-0.03823123127222061,
-0.036151520907878876,
-0.03184397518634796,
0.019521474838256836,
0.018854543566703796,
0.07777872681617737,
0.06640326231718063,
-0.025496819987893105,
0.0012616657186299562,
0.05401705205440521,
-0.01470242626965046,
-0.005363528151065111,
-0.0061660632491111755,
-0.02902061492204666,
-0.021458465605974197,
0.1330609768629074,
-0.024013511836528778,
-0.05271091312170029,
0.05160261318087578,
0.014423617161810398,
-0.005458915140479803,
-0.03308386728167534,
0.06283075362443924,
-0.041259799152612686,
-0.009691057726740837,
0.0992872565984726,
-0.05533852428197861,
0.04977431148290634,
0.029850736260414124,
-0.03847669064998627,
-0.041994571685791016,
0.012414543889462948,
0.034673839807510376,
0.014360279776155949,
-0.00667140306904912,
-0.06749200075864792,
0.02369149774312973,
0.06417704373598099,
0.005614775698632002,
-0.11143483221530914,
-0.052961237728595734,
-0.011119313538074493,
0.02285546436905861,
-0.047640763223171234,
0.009406068362295628,
0.034325938671827316,
0.029507504776120186,
-0.008872770704329014,
0.01269740704447031,
-0.06338304281234741,
0.006305185612291098,
0.009260955266654491,
0.02709382399916649,
-0.014211275614798069,
-0.011064770631492138,
-0.032920319586992264,
0.083704873919487,
0.02825475111603737
] |
PMID:8217 | The action of a binary nonionic detergent on a kidney membrane fraction. | The disruption of a kidney cortex microsomal membrane preparation by a binary, nonionic detergent, was followed by using as markers, the changes in total protein content, and (Na+, K+)-ATPase in a supernatant fraction. Both markers responded similarly to changes in pH, microsome concentration and detergent concentration, but responded differently for time-dependent studies. The (Na+, K+)-ATPase activity was increased 2.2-fold (76.1 mumoles Pi/mg protein/h, 95% ouabain-sensitive) by a single detergent treatment and 3.5-fold (92% ouabain-sensitive) by a sequential detergent treatment. Changes in the critical micelle concentration (cmc) were observed for varying detergent and protein concentrations, which suggest interactions of monomeric detergent with the membrane. The peak of (Na+, K+)-ATPase activity occurred above the cmc which suggests the participation of micelles in releasing the enzyme from the membranes. Hill plots of the protein released as the detergent concentration was varied showed a change in the slope near the cmc indicating a four-fold increase in the binding of detergent to membranes as the detergent concentration is increased above the cmc. These results suggest that the disruption of membranes by detergent involves the binding of detergent monomers to the membrane followed by the formation of co-micelles of the detergent with segments of the membrane to complete the separation process. | The action of a binary nonionic detergent on a kidney membrane fraction. The disruption of a kidney cortex microsomal membrane preparation by a binary, nonionic detergent, was followed by using as markers, the changes in total protein content, and (Na+, K+)-ATPase in a supernatant fraction. Both markers responded similarly to changes in pH, microsome concentration and detergent concentration, but responded differently for time-dependent studies. The (Na+, K+)-ATPase activity was increased 2.2-fold (76.1 mumoles Pi/mg protein/h, 95% ouabain-sensitive) by a single detergent treatment and 3.5-fold (92% ouabain-sensitive) by a sequential detergent treatment. Changes in the critical micelle concentration (cmc) were observed for varying detergent and protein concentrations, which suggest interactions of monomeric detergent with the membrane. The peak of (Na+, K+)-ATPase activity occurred above the cmc which suggests the participation of micelles in releasing the enzyme from the membranes. Hill plots of the protein released as the detergent concentration was varied showed a change in the slope near the cmc indicating a four-fold increase in the binding of detergent to membranes as the detergent concentration is increased above the cmc. These results suggest that the disruption of membranes by detergent involves the binding of detergent monomers to the membrane followed by the formation of co-micelles of the detergent with segments of the membrane to complete the separation process. | [
-0.07472733408212662,
0.0038350510876625776,
-0.01925187185406685,
-0.03399656340479851,
-0.019363492727279663,
-0.048371534794569016,
0.062463440001010895,
0.021979959681630135,
-0.035606853663921356,
-0.02776394784450531,
-0.003624721895903349,
-0.06997505575418472,
-0.018656885251402855,
0.03704214096069336,
0.008174478076398373,
-0.022057414054870605,
0.041011448949575424,
-0.0004942217492498457,
-0.07812044769525528,
0.0031342327129095793,
-0.008313396945595741,
-0.03461626172065735,
0.04333445429801941,
-0.0009278831421397626,
0.014447607100009918,
-0.02991349995136261,
0.007309598848223686,
-0.008983936160802841,
-0.07774081081151962,
-0.24416835606098175,
0.021999360993504524,
-0.026691406965255737,
-0.01296564843505621,
-0.031182296574115753,
-0.004453857894986868,
0.00034764912561513484,
-0.008201127871870995,
-0.040324870496988297,
-0.007014944218099117,
0.044833626598119736,
0.06329269707202911,
0.0036128971260041,
-0.006438767537474632,
0.011718709021806717,
-0.01947801187634468,
-0.012541116215288639,
0.03781188651919365,
0.0022521959617733955,
0.11136455833911896,
-0.008986227214336395,
-0.008614100515842438,
0.0025654989294707775,
-0.005871186498552561,
0.04492008313536644,
0.005655786953866482,
0.0022295755334198475,
0.02327420935034752,
0.020856687799096107,
0.0374489463865757,
0.03246855363249779,
0.031726665794849396,
0.004561746958643198,
-0.1552780121564865,
0.08334432542324066,
0.09046626091003418,
0.014819156378507614,
0.018801001831889153,
-0.004188422579318285,
0.07152862846851349,
0.05986802652478218,
-0.035527292639017105,
0.049508415162563324,
-0.003647645004093647,
0.02395579405128956,
0.03624283894896507,
-0.0250450037419796,
0.0008478576201014221,
0.03278905898332596,
0.017075123265385628,
0.026535823941230774,
-0.044259924441576004,
-0.02817332185804844,
0.006622933316975832,
-0.02371961809694767,
0.0007408859673887491,
0.05572222173213959,
-0.036891378462314606,
-0.03420810028910637,
0.04497426003217697,
-0.019948240369558334,
-0.026971105486154556,
-0.026253776624798775,
-0.03814219683408737,
-0.05095893144607544,
-0.022628353908658028,
0.037248946726322174,
-0.007246849127113819,
-0.034828636795282364,
0.006468737963587046,
0.40415146946907043,
-0.016801388934254646,
0.02286534756422043,
-0.045224592089653015,
-0.051903679966926575,
-0.022743383422493935,
-0.030582524836063385,
0.017397116869688034,
-0.021361839026212692,
0.01980402320623398,
0.03101876936852932,
0.026254422962665558,
-0.02037433534860611,
0.03178126737475395,
-0.02355012111365795,
-0.024472102522850037,
0.011999590322375298,
0.02739962376654148,
-0.025245176628232002,
-0.00504125002771616,
0.09601530432701111,
-0.03346296027302742,
-0.03527390956878662,
0.0360611155629158,
-0.036536797881126404,
0.011736677028238773,
0.027280882000923157,
-0.004941868595778942,
0.10402952879667282,
0.04802070930600166,
0.011023211292922497,
0.0030100932344794273,
0.026484934613108635,
-0.03947024792432785,
0.013314543291926384,
0.04608817398548126,
-0.04229569435119629,
0.030942881479859352,
-0.07789143174886703,
0.017081525176763535,
-0.0004036355239804834,
-0.02492976002395153,
-0.012998485937714577,
-0.0775466337800026,
-0.07901559770107269,
-0.0019699090626090765,
0.09623289853334427,
0.04055450111627579,
0.02424628660082817,
-0.0030250318814069033,
0.014548148028552532,
0.0015100238379091024,
0.0428372286260128,
-0.03734767809510231,
0.03281962126493454,
-0.010653899051249027,
-0.017637411132454872,
0.05216487497091293,
0.01348266564309597,
-0.0032020348589867353,
-0.0007091019069775939,
-0.0625111386179924,
-0.04375690594315529,
0.04474867880344391,
0.07401509582996368,
0.014401218853890896,
0.008162248879671097,
-0.026286212727427483,
-0.0255875326693058,
-0.035511478781700134,
0.011666493490338326,
0.002564937574788928,
-0.051387492567300797,
0.023358220234513283,
-0.06584740430116653,
-0.018673287704586983,
0.006678259465843439,
0.02615159936249256,
-0.005579716060310602,
0.04435480013489723,
-0.04997655376791954,
-0.03777417913079262,
0.015359547920525074,
-0.027144385501742363,
0.02567276731133461,
0.039235420525074005,
-0.017437338829040527,
-0.030057258903980255,
0.0178693737834692,
0.02627425082027912,
0.09191165864467621,
-0.04041373357176781,
-0.007823615334928036,
-0.07929276674985886,
-0.011088539846241474,
-0.03136974200606346,
-0.07157965749502182,
-0.02787776105105877,
-0.018826870247721672,
-0.03275832533836365,
-0.020157340914011,
0.09855034202337265,
0.02836514450609684,
-0.0038601509295403957,
0.05870901048183441,
0.017293768003582954,
0.03986918553709984,
0.03297824785113335,
0.027924787253141403,
0.06723417341709137,
0.07993734627962112,
0.0037975714076310396,
-0.010785277932882309,
-0.08190212398767471,
0.011183169670403004,
-0.02720686048269272,
0.0669168159365654,
0.018738364800810814,
-0.001270551816560328,
0.10859600454568863,
0.003218411933630705,
0.027370290830731392,
-0.017692623659968376,
-0.06819827109575272,
-0.31055620312690735,
-0.03582308441400528,
0.014001184143126011,
0.010317238979041576,
0.08947298675775528,
-0.0051100426353514194,
0.0420035645365715,
0.03803738206624985,
-0.015956643968820572,
-0.09619113802909851,
0.04257862642407417,
0.020346514880657196,
-0.03658965602517128,
-0.006730389315634966,
-0.024991605430841446,
0.006729348562657833,
0.0011179641587659717,
0.003954342566430569,
0.04393409565091133,
0.04637191817164421,
0.002395041286945343,
-0.013841472566127777,
0.019469302147626877,
0.04259740933775902,
0.09322162717580795,
-0.03603646531701088,
0.08525100350379944,
0.016803672537207603,
-0.012071321718394756,
0.009209593757987022,
-0.025322699919342995,
0.024196960031986237,
-0.04548810422420502,
-0.1014435812830925,
0.0006900465814396739,
-0.0029170610941946507,
-0.015362994745373726,
-0.03720495104789734,
-0.013477092608809471,
-0.023499201983213425,
-0.07802723348140717,
0.010689955204725266,
-0.04002831131219864,
-0.05387624725699425,
-0.06399936228990555,
-0.040180109441280365,
0.011042039841413498,
0.05135055258870125,
-0.04398726671934128,
-0.02087981626391411,
0.04408097267150879,
-0.012334811501204967,
-0.0036514035891741514,
-0.05613528937101364,
0.024031339213252068,
-0.009368105791509151,
0.03379770368337631,
-0.044520340859889984,
-0.025617895647883415,
-0.0016179748345166445,
-0.015600417740643024,
-0.00005125708048581146,
-0.007182269357144833,
-0.0013390802778303623,
-0.011623351834714413,
0.02548953704535961,
0.01853605918586254,
-0.03757438436150551,
0.031127601861953735,
-0.03307417780160904,
-0.02267874963581562,
0.117282934486866,
0.04627920314669609,
0.008866997435688972,
0.018231339752674103,
0.045415252447128296,
-0.05627402663230896,
-0.043405719101428986,
-0.08626509457826614,
0.04031747207045555,
0.013771065510809422,
-0.007926317863166332,
0.06053490191698074,
-0.02941642887890339,
-0.04202555865049362,
0.03857418894767761,
-0.0021928066853433847,
0.0004778100992552936,
0.017490025609731674,
0.00802532583475113,
-0.011145029217004776,
-0.014208398759365082,
-0.050118185579776764,
-0.003574978793039918,
0.040474727749824524,
0.03694908320903778,
-0.28328195214271545,
0.02623978815972805,
0.028981314972043037,
0.03258645534515381,
0.04199545830488205,
-0.016704250127077103,
0.05457253009080887,
-0.03789610415697098,
-0.01139887049794197,
0.07222320139408112,
-0.01653141900897026,
0.011201098561286926,
0.015063242986798286,
0.04503234475851059,
0.0027640669140964746,
0.018681367859244347,
0.05047120526432991,
-0.04269785061478615,
0.008881719782948494,
-0.0037243764381855726,
0.03969275951385498,
-0.015435495413839817,
0.15120814740657806,
0.02239709347486496,
-0.0010419985046610236,
0.04201888293027878,
-0.01796499826014042,
0.0046366429887712,
-0.05890883505344391,
-0.012225673533976078,
-0.002673689741641283,
0.02394968457520008,
0.031254351139068604,
-0.030014673247933388,
0.022390397265553474,
-0.004351917188614607,
-0.042084790766239166,
0.00017040756938513368,
-0.01049348246306181,
0.01512354239821434,
0.04190843552350998,
-0.01215167623013258,
-0.06921786069869995,
-0.013934708200395107,
0.08652974665164948,
-0.02432517521083355,
-0.07538486272096634,
-0.006063580978661776,
0.0260184183716774,
0.012115941382944584,
-0.01870226301252842,
-0.018780533224344254,
-0.0012075394624844193,
0.04008905217051506,
0.023364735767245293,
0.0036646451335400343,
-0.06936972588300705,
-0.0020248116925358772,
-0.02996499091386795,
0.03615077584981918,
0.006582747213542461,
-0.011513879522681236,
0.007461709436029196,
0.031137118116021156,
0.032089654356241226
] |
PMID:8218 | Resistance of alkylated DNA to degradation by deoxyribonuclease II at neutral and acid pH. | Methylation of a calf thymus DNA substrate by dimethyl sulphate (DMS) leads to an inhibition of deoxyribonuclease II activity which is gradually lost with time. The extent of this initial inhibition is linearly related to the amount of methylated products in DNA and quantitatively similar effects were found when the enzyme was used under either acid or neutral conditions. Deoxyribonuclease II was shown to produce 3'-phosphate termini under both acid and neutral conditions and thus, irrespective of the ionic conditions for the action of this enzyme in vivo the effects demonstrated here are of potential significance. Local denaturation of the methylated DNA may be partly responsible for these inhibitory effects but it is likely that the methyl purines also play a more direct role. | Resistance of alkylated DNA to degradation by deoxyribonuclease II at neutral and acid pH. Methylation of a calf thymus DNA substrate by dimethyl sulphate (DMS) leads to an inhibition of deoxyribonuclease II activity which is gradually lost with time. The extent of this initial inhibition is linearly related to the amount of methylated products in DNA and quantitatively similar effects were found when the enzyme was used under either acid or neutral conditions. Deoxyribonuclease II was shown to produce 3'-phosphate termini under both acid and neutral conditions and thus, irrespective of the ionic conditions for the action of this enzyme in vivo the effects demonstrated here are of potential significance. Local denaturation of the methylated DNA may be partly responsible for these inhibitory effects but it is likely that the methyl purines also play a more direct role. | [
-0.07916934043169022,
0.016351774334907532,
-0.030142342671751976,
0.018706263974308968,
-0.02271547168493271,
0.00379408011212945,
0.029746370390057564,
0.046645767986774445,
-0.05122797191143036,
-0.046789754182100296,
0.01094565074890852,
-0.05705248937010765,
0.044026248157024384,
-0.028043724596500397,
0.007937295362353325,
-0.03988294303417206,
-0.02122754417359829,
0.03260711580514908,
-0.05932709947228432,
-0.023142574355006218,
-0.020117487758398056,
0.08076336979866028,
0.03374222666025162,
0.003718070685863495,
0.013281077146530151,
-0.012826300226151943,
0.0018407739698886871,
0.04430656135082245,
-0.0786195620894432,
-0.21238288283348083,
0.02399221807718277,
-0.010877607390284538,
-0.00949668325483799,
-0.01922275498509407,
-0.018660524860024452,
-0.01570884883403778,
-0.03177117928862572,
-0.012384388595819473,
-0.05776936188340187,
0.04150683805346489,
0.023079318925738335,
-0.017329366877675056,
0.061898354440927505,
0.009959311224520206,
-0.0504801906645298,
0.05058532580733299,
-0.04843607544898987,
-0.029286572709679604,
0.021137112751603127,
0.030988654121756554,
0.019373515620827675,
0.014416812919080257,
0.02672206051647663,
0.014487039297819138,
-0.010274642147123814,
0.012286130338907242,
0.0401327908039093,
0.06400293856859207,
-0.023150989785790443,
0.006966912187635899,
0.02959686703979969,
-0.0011443173279985785,
-0.11466596275568008,
0.036738600581884384,
0.08966627717018127,
0.043593425303697586,
0.04711795598268509,
-0.04928295686841011,
0.0491349920630455,
0.04405350610613823,
-0.07970912754535675,
0.03570447489619255,
0.0016201287508010864,
0.03710770234465599,
0.05064947158098221,
-0.0031614615581929684,
-0.005514547694474459,
0.01716947928071022,
0.07979835569858551,
-0.046877048909664154,
-0.030877744778990746,
-0.0403502956032753,
0.04406368359923363,
-0.07185696810483932,
-0.025943206623196602,
0.021130209788680077,
0.036083877086639404,
-0.030507218092679977,
-0.009548661299049854,
-0.006702964194118977,
0.04888072609901428,
-0.04316047206521034,
-0.01327340118587017,
-0.03922811895608902,
-0.07502593845129013,
0.06593496352434158,
0.07021338492631912,
-0.0038022161461412907,
0.01860100030899048,
0.38333195447921753,
-0.03225331753492355,
0.026410019025206566,
-0.07732771337032318,
-0.025238854810595512,
-0.031905997544527054,
-0.015024351887404919,
-0.04144059494137764,
-0.014668631367385387,
0.0015205265954136848,
0.019490540027618408,
0.08527851104736328,
0.06979000568389893,
0.01052656676620245,
-0.02599542774260044,
0.002827635733410716,
0.019693832844495773,
0.012004617601633072,
0.0034133759327232838,
-0.05049318075180054,
0.04777943715453148,
-0.04466843605041504,
0.010828888975083828,
-0.004197631496936083,
0.014459486119449139,
0.011153456754982471,
0.03355017304420471,
0.03285914659500122,
0.11892010271549225,
0.03800442814826965,
-0.0329536497592926,
0.0038570647593587637,
-0.053588759154081345,
-0.0225309357047081,
0.03247606381773949,
0.013638070784509182,
-0.014251473359763622,
0.014307506382465363,
0.002677636221051216,
0.05090143531560898,
-0.021837344393134117,
-0.06906387954950333,
-0.05073544383049011,
-0.07661812007427216,
-0.05027099698781967,
-0.0017234638798981905,
0.0200367234647274,
-0.024784650653600693,
0.03361062332987785,
0.019431285560131073,
0.024745874106884003,
0.008499621413648129,
0.051106881350278854,
-0.035386595875024796,
0.01686173677444458,
-0.003074819454923272,
0.06304722279310226,
-0.03951437398791313,
-0.04330797865986824,
0.049484990537166595,
-0.002277785912156105,
-0.03371919319033623,
0.015403619967401028,
0.01599028892815113,
0.10473879426717758,
-0.029959185048937798,
0.02974935807287693,
-0.03666946291923523,
0.02388838864862919,
-0.012121336534619331,
0.03459214046597481,
-0.0022943380754441023,
-0.04908687248826027,
-0.007288486231118441,
-0.08586444705724716,
-0.05564209818840027,
-0.016316834837198257,
0.021323267370462418,
0.028720617294311523,
0.006206319201737642,
0.005303679965436459,
0.042180921882390976,
-0.025834674015641212,
-0.03995409607887268,
-0.0010567228309810162,
0.08605021983385086,
-0.04141378402709961,
-0.07869499176740646,
0.047631073743104935,
0.03828312084078789,
0.06438949704170227,
-0.0010711309732869267,
-0.03399886190891266,
-0.0672624260187149,
-0.027953268960118294,
-0.041225139051675797,
-0.06953690201044083,
-0.01589149981737137,
0.037087101489305496,
-0.03507603704929352,
0.0052872453816235065,
0.08346395939588547,
0.07977215945720673,
0.0061624301597476006,
0.04315263405442238,
0.019270509481430054,
-0.005236463621258736,
0.010093148797750473,
0.004824941977858543,
0.023476852104067802,
-0.005463726352900267,
-0.049156755208969116,
-0.0011365284444764256,
-0.04722918942570686,
-0.01208451297134161,
-0.03926483914256096,
0.06781087815761566,
0.03618192300200462,
0.04345257952809334,
0.015009955503046513,
0.04911064729094505,
0.00438753142952919,
-0.0680517703294754,
-0.0780862420797348,
-0.3038460314273834,
-0.03938073664903641,
-0.022527392953634262,
0.005772160366177559,
-0.02207179181277752,
-0.030628308653831482,
-0.008238943293690681,
-0.02612929977476597,
0.012503390200436115,
-0.04437088593840599,
0.027019334957003593,
0.04594769701361656,
-0.008014239370822906,
-0.07554713636636734,
0.005741728004068136,
0.008332561701536179,
0.009853030554950237,
0.016300564631819725,
0.030811622738838196,
0.004323930945247412,
0.035682469606399536,
-0.008758879266679287,
0.06181322783231735,
-0.01529301330447197,
0.11137747764587402,
0.04665103927254677,
0.09259538352489471,
-0.005141488742083311,
0.017649861052632332,
-0.0008079301915131509,
0.051483526825904846,
0.07529397308826447,
-0.023409990593791008,
-0.07444069534540176,
-0.024728761985898018,
-0.07588070631027222,
-0.05197829380631447,
-0.01929011195898056,
0.04179953783750534,
0.0017955927178263664,
0.018193639814853668,
-0.02847740799188614,
-0.017868848517537117,
-0.05735114961862564,
-0.033026013523340225,
-0.03369000926613808,
0.015903959050774574,
0.029377669095993042,
0.03526126593351364,
-0.05896221473813057,
0.045886535197496414,
0.04250337928533554,
0.014617543667554855,
-0.01815551146864891,
-0.009318195283412933,
-0.009541922248899937,
-0.06528069078922272,
-0.007193691097199917,
-0.03631100803613663,
-0.04940718039870262,
-0.0012238317867740989,
-0.009604403749108315,
-0.014229780063033104,
0.05641830340027809,
-0.037200938910245895,
-0.0042077540419995785,
0.05284906178712845,
-0.013972588814795017,
0.023446664214134216,
-0.004932499025017023,
0.024827513843774796,
0.09799991548061371,
-0.023074952885508537,
-0.02130754478275776,
-0.011377023532986641,
0.05131944641470909,
-0.08198841661214828,
-0.027774207293987274,
-0.019944969564676285,
0.020590633153915405,
0.028465604409575462,
-0.007532805670052767,
0.0715540200471878,
-0.03117184154689312,
-0.035959262400865555,
0.06573538482189178,
0.032862741500139236,
-0.018376382067799568,
0.030292607843875885,
0.048015326261520386,
0.023098157718777657,
-0.026040857657790184,
-0.003527425229549408,
0.015672631561756134,
0.03070848062634468,
0.005825493950396776,
-0.25094130635261536,
-0.04718201234936714,
-0.004159394185990095,
0.022157812491059303,
-0.03894665464758873,
0.04285585135221481,
0.027778996154665947,
0.02032589353621006,
-0.044519539922475815,
0.03877405822277069,
0.007733795791864395,
0.03241047263145447,
0.050586868077516556,
0.058214399963617325,
-0.010431341826915741,
0.027922900393605232,
0.05248670279979706,
-0.015571375377476215,
-0.03296507149934769,
-0.04820365086197853,
-0.0100437356159091,
-0.05892229452729225,
0.1769171506166458,
-0.010838368907570839,
-0.015483126044273376,
0.0846371203660965,
-0.017997359856963158,
0.04218021035194397,
-0.0441448912024498,
0.0065820771269500256,
-0.030509771779179573,
-0.01107655931264162,
0.06366367638111115,
-0.04393250122666359,
0.008578712120652199,
0.03216737508773804,
-0.034457363188266754,
-0.03179335966706276,
0.0012448173947632313,
0.022831302136182785,
-0.040591850876808167,
0.0015969924861565232,
-0.0896848514676094,
-0.03583794832229614,
0.08984633535146713,
-0.09013224393129349,
-0.04266277700662613,
-0.03057386353611946,
0.050206832587718964,
-0.0080953910946846,
-0.018554741516709328,
0.01749589294195175,
0.01597500964999199,
-0.03502397611737251,
-0.004265944007784128,
-0.03778254985809326,
-0.02867806889116764,
-0.0004544277617242187,
0.0692887157201767,
-0.010111797600984573,
0.02227034606039524,
0.0280336681753397,
-0.0012368559837341309,
0.04432794824242592,
0.025528976693749428
] |
PMID:8219 | Enzymatic properties of native and N-ethylmaleimide-modified cardiac myosin from normal and thyrotoxic rabbits. | Cardiac myosin from thyrotoxic animals (myosin-T) exhibits elevated Ca2+ -ATPase activity which is resistant to further stimulation by sulfhydryl modification. In the present study, we have compared the enzymatic properties of myosin-T with those of myosin from euthyroid rabbits (myosin-N) and the derivatives of myosin-T and myosin-N formed by blocking the most rapidly reacting class of thiols (SH1) with N-ethylmaleimide (NEM). Vmax for Ca2+ -ATPase of myosin-T was about 250% greater than myosin-N and was nearly the same as NEM-modified myosin-N. Values for the apparent Km of myosin-T and NEM-modified myosin-N were 200% greater than the value for unmodified myosin-N. Vmax and Km for K+ (EDTA)-ATPase activity of NEM-modified myosin-T and myosin-N were identical. The Ca2+ saturation, pH, and salt-dependency curves for the ATPase activity of myosin-T were parallel to the curves for myosin-N and differed from those for the NEM-modified myosins. Myosin-T exhibited an increased rate of hydrolysis of ATP, CTP, and UTP in both low (0.05m) and high (0.5m) KCl medium. NEM-modified myosin-N showed increased hydrolysis of ATP and CTP in low KCl medium and increased hydrolysis of ATP, CTP, and UTP in high KCl medium. These results support the hypothesis that the enzymatic behavior of myosin-T may be caused by an alteration in the active site near the SH, thiols. The unique enzymatic properties of myosin-T did not seem to be the result of a major change in structure. The electrophoretic pattern of light chains from myosin-T and myosin-N was the same in polyacrylamide gels containing either 8 M urea at pH 8.6 or sodium dodecyl sulfate. Also, myosin-T had a normal amino acid composition and lacked 3-methyl-histidine and hot acid-stable phosphate. | Enzymatic properties of native and N-ethylmaleimide-modified cardiac myosin from normal and thyrotoxic rabbits. Cardiac myosin from thyrotoxic animals (myosin-T) exhibits elevated Ca2+ -ATPase activity which is resistant to further stimulation by sulfhydryl modification. In the present study, we have compared the enzymatic properties of myosin-T with those of myosin from euthyroid rabbits (myosin-N) and the derivatives of myosin-T and myosin-N formed by blocking the most rapidly reacting class of thiols (SH1) with N-ethylmaleimide (NEM). Vmax for Ca2+ -ATPase of myosin-T was about 250% greater than myosin-N and was nearly the same as NEM-modified myosin-N. Values for the apparent Km of myosin-T and NEM-modified myosin-N were 200% greater than the value for unmodified myosin-N. Vmax and Km for K+ (EDTA)-ATPase activity of NEM-modified myosin-T and myosin-N were identical. The Ca2+ saturation, pH, and salt-dependency curves for the ATPase activity of myosin-T were parallel to the curves for myosin-N and differed from those for the NEM-modified myosins. Myosin-T exhibited an increased rate of hydrolysis of ATP, CTP, and UTP in both low (0.05m) and high (0.5m) KCl medium. NEM-modified myosin-N showed increased hydrolysis of ATP and CTP in low KCl medium and increased hydrolysis of ATP, CTP, and UTP in high KCl medium. These results support the hypothesis that the enzymatic behavior of myosin-T may be caused by an alteration in the active site near the SH, thiols. The unique enzymatic properties of myosin-T did not seem to be the result of a major change in structure. The electrophoretic pattern of light chains from myosin-T and myosin-N was the same in polyacrylamide gels containing either 8 M urea at pH 8.6 or sodium dodecyl sulfate. Also, myosin-T had a normal amino acid composition and lacked 3-methyl-histidine and hot acid-stable phosphate. | [
-0.0727551281452179,
-0.04535997658967972,
0.018611589446663857,
0.048527687788009644,
0.022596536204218864,
-0.02119542844593525,
0.04746498912572861,
0.05021492391824722,
-0.03445997089147568,
-0.02104894444346428,
0.01597050204873085,
-0.07191140204668045,
-0.040036674588918686,
0.0015366047155112028,
-0.005370450206100941,
-0.06179818883538246,
0.03214668482542038,
0.0041937860660254955,
-0.06039571017026901,
-0.01967831701040268,
-0.03984283283352852,
-0.006713507231324911,
0.030141569674015045,
-0.032948996871709824,
0.047092314809560776,
0.04172152280807495,
-0.006808800157159567,
0.06993312388658524,
-0.04012181982398033,
-0.2034226804971695,
0.011509482748806477,
-0.06648583710193634,
-0.004239426925778389,
0.037154801189899445,
0.006953696254640818,
0.021673882380127907,
-0.046649206429719925,
-0.08558201789855957,
-0.028712091967463493,
0.0371403694152832,
0.06325648725032806,
0.004954033065587282,
-0.0010798199800774455,
0.0230344757437706,
0.07275369763374329,
0.03387385234236717,
-0.022274166345596313,
-0.026363886892795563,
0.06917526572942734,
0.060202352702617645,
-0.014860140159726143,
-0.016057707369327545,
0.04330449923872948,
0.04727622866630554,
0.0497654564678669,
-0.01732727326452732,
0.047556646168231964,
0.0708792433142662,
0.0058609116822481155,
-0.014652633108198643,
0.06927349418401718,
0.06315387785434723,
-0.1269766390323639,
0.08208136260509491,
0.05106271430850029,
0.00826710369437933,
0.014066587202250957,
0.006484017241746187,
0.018495889380574226,
0.03680543228983879,
-0.022447042167186737,
0.08181837201118469,
0.021902548149228096,
0.0431881844997406,
-0.038603369146585464,
0.03348567709326744,
0.02592281997203827,
-0.00764446659013629,
0.025230765342712402,
0.0405367836356163,
-0.03356916084885597,
-0.010243489407002926,
0.06471788138151169,
-0.059805724769830704,
0.01708214171230793,
0.056922756135463715,
0.0012547458754852414,
-0.047372207045555115,
0.0017252926481887698,
-0.0022718077525496483,
-0.022916311398148537,
-0.027786899358034134,
-0.010312449187040329,
-0.052962448447942734,
-0.049124862998723984,
0.06787635385990143,
0.011067640967667103,
-0.00241537531837821,
0.0033504932653158903,
0.3734123110771179,
0.028474701568484306,
0.029985032975673676,
-0.07118930667638779,
0.008303932845592499,
-0.005789851304143667,
-0.020391572266817093,
-0.028455009683966637,
-0.010604888200759888,
-0.0012035223189741373,
0.056100938469171524,
0.03822153061628342,
0.007928423583507538,
-0.019217312335968018,
-0.04178581386804581,
0.0008387163397856057,
0.009968544356524944,
0.02504650503396988,
-0.03924887999892235,
0.003140917746350169,
0.07810775935649872,
-0.06970293819904327,
0.020909391343593597,
0.029907027259469032,
-0.04725661128759384,
0.009600614197552204,
-0.011757773347198963,
-0.050066739320755005,
0.07957494258880615,
0.08995907753705978,
0.005589036736637354,
0.03603338077664375,
0.05320029333233833,
-0.040978409349918365,
-0.0173583272844553,
0.02843254990875721,
-0.03577748313546181,
0.025467580184340477,
-0.06909473240375519,
-0.012620423920452595,
-0.009021234698593616,
-0.05319126695394516,
-0.08422855287790298,
-0.03995436802506447,
-0.06720751523971558,
-0.04589301347732544,
0.08254945278167725,
-0.00019137187337037176,
0.012936641462147236,
-0.019876303151249886,
0.0188360046595335,
-0.005490320734679699,
0.044561564922332764,
-0.01908380351960659,
0.029355067759752274,
-0.06153963506221771,
-0.00476198922842741,
-0.004062292631715536,
0.02376525104045868,
-0.0013429307145997882,
-0.0717921257019043,
-0.08213331550359726,
-0.00844458770006895,
-0.0015738188521936536,
0.07323092967271805,
0.03904551640152931,
-0.04825596883893013,
-0.032792575657367706,
0.019027259200811386,
0.03534257039427757,
-0.030387919396162033,
0.003367120400071144,
0.0017318256432190537,
-0.029562050476670265,
0.0009610652341507375,
-0.04405912384390831,
-0.009877365082502365,
-0.00007840389298507944,
-0.018660081550478935,
0.03803619369864464,
0.034567736089229584,
0.029565710574388504,
-0.023639706894755363,
0.011144396848976612,
0.042711421847343445,
0.03859824314713478,
0.0035035470500588417,
-0.011849780566990376,
0.058654189109802246,
0.030483050271868706,
0.07855037599802017,
-0.0010510821593925357,
0.0015385602600872517,
-0.07718302309513092,
0.030484499409794807,
-0.07243691384792328,
-0.048010896891355515,
-0.024623850360512733,
0.06160761043429375,
0.039211101830005646,
0.0010950906435027719,
0.07276073843240738,
0.012129643931984901,
0.02579401060938835,
0.07848469913005829,
-0.03898656368255615,
0.03485206142067909,
-0.038298800587654114,
-0.010862225666642189,
0.037012167274951935,
-0.009352186694741249,
-0.051661960780620575,
-0.05967888608574867,
-0.05985964089632034,
-0.017993910238146782,
-0.06616123765707016,
0.03345056623220444,
0.005289154127240181,
0.02476663887500763,
0.06442034989595413,
-0.027808092534542084,
0.029533805325627327,
-0.04157010838389397,
-0.061314649879932404,
-0.30090004205703735,
-0.04195212945342064,
0.005161721259355545,
0.01051841489970684,
0.055130694061517715,
-0.0564129613339901,
0.003465525107458234,
-0.01269291527569294,
0.034995876252651215,
-0.08163858205080032,
-0.01281423307955265,
0.023815495893359184,
-0.0697222650051117,
-0.08126507699489594,
0.045871347188949585,
0.060374438762664795,
0.017062801867723465,
-0.01875203102827072,
0.07598395645618439,
0.009050020016729832,
0.0032201178837567568,
0.018517324700951576,
0.012604699470102787,
-0.0035673831589519978,
0.07004555314779282,
-0.008830031380057335,
0.0864107683300972,
-0.008021002635359764,
-0.0044420636259019375,
0.02765680104494095,
0.014909204095602036,
0.02321665547788143,
-0.042886629700660706,
-0.0830717533826828,
0.022132549434900284,
0.005819329991936684,
-0.020940730348229408,
-0.010191231966018677,
0.031161289662122726,
-0.05256417393684387,
-0.0679001733660698,
0.0007783905602991581,
-0.007070041261613369,
-0.0870489776134491,
-0.03248859569430351,
-0.04452184587717056,
0.016267703846096992,
0.03132300823926926,
0.019512495025992393,
-0.037992704659700394,
0.03646055608987808,
0.006504829972982407,
0.010555392131209373,
-0.02753720059990883,
0.01799209974706173,
-0.01029159501194954,
-0.04688994958996773,
0.014068801887333393,
0.0054770056158304214,
-0.017331840470433235,
-0.044702235609292984,
0.008120604790747166,
0.014019974507391453,
0.0188995860517025,
0.010738511569797993,
-0.036673661321401596,
0.02405688352882862,
-0.023510366678237915,
0.045299142599105835,
-0.04730742797255516,
0.0017577321268618107,
0.08408486843109131,
0.011552346870303154,
0.019935663789510727,
0.06862647831439972,
0.01250675693154335,
-0.035055238753557205,
0.015494137071073055,
0.00439685583114624,
0.07701610773801804,
0.005037958733737469,
-0.017990630120038986,
0.09673713147640228,
-0.04386824741959572,
-0.036999065428972244,
0.004382735583931208,
0.03612374886870384,
-0.005788115318864584,
-0.013363946229219437,
0.03401041775941849,
0.015738390386104584,
-0.004192984197288752,
-0.038986895233392715,
0.004030040465295315,
0.03144585341215134,
-0.013899209909141064,
-0.2643713057041168,
-0.016859441995620728,
0.05254822596907616,
-0.005095467437058687,
-0.020793631672859192,
-0.025798754766583443,
0.00295067741535604,
-0.02737916260957718,
-0.0405602753162384,
0.028678953647613525,
-0.006231346167623997,
-0.006735227070748806,
0.07526443153619766,
0.0637594684958458,
-0.025276893749833107,
-0.011093094944953918,
0.0835341215133667,
-0.03366103023290634,
0.029624443501234055,
-0.06000505015254021,
0.025651566684246063,
-0.037735652178525925,
0.15494772791862488,
0.028661342337727547,
-0.01640663668513298,
0.05782632529735565,
-0.020259901881217957,
0.03575422614812851,
-0.02877860888838768,
-0.010706416331231594,
-0.022252721711993217,
0.021206717938184738,
0.04830063134431839,
-0.049084391444921494,
-0.013902442529797554,
0.044594570994377136,
-0.011782913468778133,
0.011269261129200459,
-0.06411450356245041,
0.02769901603460312,
-0.010039430111646652,
-0.02415051870048046,
-0.11016666889190674,
-0.07266269624233246,
0.05102227255702019,
-0.04144630581140518,
-0.045622773468494415,
-0.03561500459909439,
0.014737960882484913,
0.01306523010134697,
-0.03774270415306091,
-0.040978796780109406,
0.07262870669364929,
0.016018355265259743,
-0.012291999533772469,
-0.01608046144247055,
-0.03431212529540062,
-0.046194061636924744,
-0.0024151792749762535,
0.005870594177395105,
0.007359427399933338,
0.013837741687893867,
-0.05394003912806511,
0.09878754615783691,
0.050844427198171616
] |
PMID:8220 | The association between serum triglycerides and gamma glutamyl transpeptidase activity in diabetes mellitus. | A study conducted on 228 diabetic patients has shown a significant positive association between serum gamma-glutamyl transpeptidase (GGT) and triglyceride levels. Both fall with treatment, the most marked reduction occurring in patients on insulin. We suggest that the association between serum GGT and triglyceride levels and also the higher incidence of raised GGT and triglyceride levels in new diabetics may reflect hepatic microsomal enzyme induction of the rate-limiting enzymes of triglyceride synthesis. Serum GGT does not seem to correlate with hepatomegaly in diabetes mellitus. | The association between serum triglycerides and gamma glutamyl transpeptidase activity in diabetes mellitus. A study conducted on 228 diabetic patients has shown a significant positive association between serum gamma-glutamyl transpeptidase (GGT) and triglyceride levels. Both fall with treatment, the most marked reduction occurring in patients on insulin. We suggest that the association between serum GGT and triglyceride levels and also the higher incidence of raised GGT and triglyceride levels in new diabetics may reflect hepatic microsomal enzyme induction of the rate-limiting enzymes of triglyceride synthesis. Serum GGT does not seem to correlate with hepatomegaly in diabetes mellitus. | [
0.029947444796562195,
0.014334596693515778,
0.000608852889854461,
0.028621139004826546,
0.04064884036779404,
-0.013284610584378242,
0.048814184963703156,
0.07443848252296448,
-0.04429776966571808,
-0.05407106503844261,
-0.008390639908611774,
-0.05629190430045128,
0.0018718967912718654,
0.04626201093196869,
-0.07115387916564941,
-0.06824562698602676,
0.023015178740024567,
-0.02655649185180664,
-0.042637523263692856,
-0.03848472982645035,
-0.029486484825611115,
0.01132145244628191,
0.1017456129193306,
-0.029134226962924004,
0.057212796062231064,
-0.00775407999753952,
-0.06124811992049217,
-0.05785706639289856,
-0.0661119595170021,
-0.1461232453584671,
-0.011548132635653019,
-0.003048895625397563,
-0.01553624588996172,
0.01227316539734602,
-0.023695258423686028,
0.006781651172786951,
-0.028642602264881134,
0.03320277854800224,
-0.008662281557917595,
-0.03454694151878357,
0.02652137726545334,
-0.006348843220621347,
-0.02213829755783081,
-0.03463784232735634,
0.0415579155087471,
0.006933845113962889,
-0.032378364354372025,
0.03903939574956894,
0.016215329989790916,
0.04475538805127144,
-0.007195652928203344,
0.04097066447138786,
0.06451290845870972,
0.07821425795555115,
-0.0010102692758664489,
-0.002484395168721676,
-0.023888472467660904,
0.05526216700673103,
-0.060885485261678696,
-0.00558900460600853,
-0.015282425098121166,
0.02408864162862301,
-0.13385844230651855,
0.024244284257292747,
0.06593398749828339,
-0.03144771605730057,
-0.02390676736831665,
-0.010159080848097801,
0.021241331472992897,
-0.006505078636109829,
-0.026447199285030365,
0.04599696025252342,
-0.048433590680360794,
-0.00539212953299284,
-0.010191850364208221,
0.004979785066097975,
0.030870618298649788,
0.019067194312810898,
0.014879496768116951,
0.07515047490596771,
0.002392144873738289,
-0.026372617110610008,
0.02729797549545765,
0.0019579457584768534,
-0.0359007865190506,
-0.06146370992064476,
0.005559145472943783,
-0.014756639488041401,
0.014416913501918316,
-0.0027119291480630636,
-0.027669968083500862,
-0.0873434841632843,
-0.043891020119190216,
0.007366965524852276,
-0.09338103979825974,
0.0029418396297842264,
0.057980459183454514,
-0.03943435102701187,
-0.031225712969899178,
0.38859593868255615,
-0.02100861445069313,
-0.02530350722372532,
-0.07183374464511871,
0.030621087178587914,
-0.00533226178959012,
-0.02778712846338749,
-0.010450007393956184,
-0.06037597358226776,
-0.013486314564943314,
0.020237764343619347,
0.04102062061429024,
0.023018082603812218,
0.012759994715452194,
0.0032040425576269627,
-0.04590991139411926,
0.029746849089860916,
0.059751223772764206,
0.03345037251710892,
-0.021279552951455116,
0.08743990957736969,
-0.017181910574436188,
0.01459010411053896,
-0.00034481732291169465,
-0.03622593358159065,
0.02688591741025448,
0.022374968975782394,
-0.0460495799779892,
0.08400291949510574,
0.04957329109311104,
-0.00678627286106348,
0.05969880893826485,
0.059687282890081406,
-0.03354465216398239,
0.020466554909944534,
-0.0002773805463220924,
-0.0003204916720278561,
0.021130265668034554,
-0.007361683063209057,
0.03271225467324257,
0.04377215728163719,
-0.01738743856549263,
-0.0413382425904274,
-0.06696134060621262,
-0.020960336551070213,
-0.013900653459131718,
0.11988064646720886,
-0.010009806603193283,
0.045748695731163025,
0.03017568774521351,
-0.016135890036821365,
0.034088537096977234,
0.06424545496702194,
0.015324471518397331,
0.030322259292006493,
-0.041892338544130325,
-0.005220769438892603,
0.06284257024526596,
-0.009005451574921608,
0.05434408783912659,
-0.033862724900245667,
-0.0569007433950901,
-0.011148684658110142,
-0.011936094611883163,
0.03633499518036842,
0.06727413088083267,
-0.028377052396535873,
-0.04205187037587166,
-0.025745470076799393,
0.011001462116837502,
-0.01774466037750244,
-0.005244363099336624,
0.040972188115119934,
-0.007576742209494114,
-0.040006719529628754,
0.0499044768512249,
-0.009318068623542786,
0.048468273133039474,
0.02476676180958748,
0.0010804226621985435,
0.03715765103697777,
0.06214706972241402,
-0.0051625738851726055,
-0.058864299207925797,
0.03985707461833954,
0.003667272627353668,
-0.0026119265239685774,
-0.02791033685207367,
0.006953081581741571,
0.010237815789878368,
0.01869230531156063,
-0.05108918249607086,
0.01153748668730259,
-0.09342360496520996,
-0.003905436024069786,
-0.003748571965843439,
-0.03929414227604866,
-0.03616473451256752,
0.0009686436387710273,
0.021446866914629936,
-0.03131026774644852,
0.07644513994455338,
0.009631693363189697,
0.038018401712179184,
0.041828520596027374,
-0.04822605103254318,
0.007204005494713783,
-0.02398095838725567,
-0.034502044320106506,
0.03437129780650139,
0.03761640563607216,
0.03494754433631897,
0.020289311185479164,
-0.013956870883703232,
0.022835824638605118,
-0.031228311359882355,
0.05187234655022621,
0.005018449388444424,
0.016655422747135162,
-0.022211993113160133,
0.06699918210506439,
0.026639871299266815,
-0.045226484537124634,
-0.033012568950653076,
-0.30549198389053345,
-0.039251405745744705,
0.04244312271475792,
-0.06003209948539734,
0.029925353825092316,
-0.05159449204802513,
-0.013277917169034481,
0.0025568651035428047,
-0.05997483804821968,
0.035486143082380295,
0.06389278173446655,
0.06904992461204529,
-0.0468025766313076,
-0.06759606301784515,
-0.024128949269652367,
0.019958525896072388,
-0.03528966009616852,
-0.020136458799242973,
0.06677445769309998,
-0.005217801313847303,
0.014382364228367805,
-0.056514251977205276,
0.051891129463911057,
0.0016716413665562868,
0.08741381764411926,
-0.010815390385687351,
0.1291244775056839,
0.020090624690055847,
-0.027838366106152534,
0.0004225968732498586,
-0.010060823522508144,
0.028954070061445236,
-0.06453387439250946,
-0.06958085298538208,
0.039975136518478394,
-0.016615668311715126,
-0.05788784846663475,
-0.01512105856090784,
0.013539633713662624,
-0.014096441678702831,
-0.07356779277324677,
-0.011937471106648445,
-0.05785743147134781,
-0.01823529414832592,
-0.05178866162896156,
-0.05168953165411949,
0.01733003929257393,
0.008189240470528603,
0.04527632147073746,
0.06786312162876129,
0.06655874848365784,
-0.002639176556840539,
0.034039199352264404,
0.018788926303386688,
0.04272669553756714,
-0.045315805822610855,
-0.06425319612026215,
0.04202960804104805,
-0.04176785796880722,
0.030384061858057976,
0.004747285507619381,
0.020619820803403854,
0.022744910791516304,
0.024526508525013924,
0.019907314330339432,
-0.007526783738285303,
-0.01577630266547203,
0.002516819629818201,
0.02861672453582287,
0.10876940190792084,
0.03842124342918396,
0.09978082031011581,
-0.03601515293121338,
-0.02264031581580639,
0.01534367073327303,
0.018339665606617928,
-0.02496325969696045,
-0.059903327375650406,
-0.05908562242984772,
-0.03703205659985542,
-0.0485796220600605,
-0.043528661131858826,
0.05730616673827171,
0.018678385764360428,
-0.03812028467655182,
-0.006175521295517683,
0.04181797802448273,
0.011481450870633125,
-0.019929246976971626,
0.00884231086820364,
0.036175116896629333,
0.006109768059104681,
0.00504989642649889,
-0.07492593675851822,
-0.0415433906018734,
0.020446529611945152,
-0.2887154519557953,
0.025888098403811455,
-0.012307783588767052,
-0.016140248626470566,
-0.0007076378678902984,
-0.010948396287858486,
0.029899191111326218,
0.00007198453386081383,
-0.07704422622919083,
-0.0008219818701036274,
0.010898970067501068,
0.02690437249839306,
0.08289888501167297,
0.03920142352581024,
-0.04817577078938484,
-0.02271273359656334,
0.027252689003944397,
-0.03617241233587265,
0.017850615084171295,
-0.06760120391845703,
0.010842139832675457,
-0.0012033546809107065,
0.1639506071805954,
0.040956199169158936,
0.017032543197274208,
0.032889224588871,
0.014392262324690819,
0.005648070015013218,
0.005556260235607624,
0.04494835063815117,
-0.029765313491225243,
-0.0019049193942919374,
0.090850330889225,
-0.02501956932246685,
0.01855282485485077,
0.10899586975574493,
0.021716997027397156,
-0.010913127101957798,
-0.029651137068867683,
0.017933439463377,
0.03932226076722145,
0.03366043418645859,
-0.04849306121468544,
-0.04254235699772835,
0.03288958594202995,
-0.065155990421772,
0.004450349602848291,
0.0010994344484061003,
0.0077485512010753155,
0.015643559396266937,
0.01943383365869522,
-0.046396248042583466,
-0.045134857296943665,
-0.01772499270737171,
0.029610924422740936,
-0.06161271408200264,
-0.023313384503126144,
0.023243077099323273,
-0.03437482938170433,
0.01538117416203022,
-0.04114503413438797,
0.03274236619472504,
-0.09395015239715576,
0.03935529664158821,
0.02067335695028305
] |
PMID:8221 | Reverse-phase chromatography of polar biological substances: separation of catechol compounds by high-performance liquid chromatography. | Catecholamines and their metabolites have been separated isocratically by reverse-phase chromatography with aqueous (no organic solvent admixed) eluents. Unlike ion-exchange or ion-pair chromatography, mixtures of both acidic and basic substances can be separated in a single chromatographic run, because the retention is governed by hydrophobic interactions between the nonpolar moiety of the solute molecules and the octadecyl-silica stationary phase. The relative retention values strongly depend on the pH of the eluent, which governs the degree of dissociation of ionogenic solutes. The reproducibility of the results and the stability and efficiency of the chromatographic systems make this approach particularly attractive for use in clinical analysis. | Reverse-phase chromatography of polar biological substances: separation of catechol compounds by high-performance liquid chromatography. Catecholamines and their metabolites have been separated isocratically by reverse-phase chromatography with aqueous (no organic solvent admixed) eluents. Unlike ion-exchange or ion-pair chromatography, mixtures of both acidic and basic substances can be separated in a single chromatographic run, because the retention is governed by hydrophobic interactions between the nonpolar moiety of the solute molecules and the octadecyl-silica stationary phase. The relative retention values strongly depend on the pH of the eluent, which governs the degree of dissociation of ionogenic solutes. The reproducibility of the results and the stability and efficiency of the chromatographic systems make this approach particularly attractive for use in clinical analysis. | [
-0.019117288291454315,
0.01524503156542778,
-0.026862511411309242,
0.0023771687410771847,
0.01996913179755211,
-0.02413860894739628,
0.04952896013855934,
0.014023730531334877,
-0.021611586213111877,
-0.028319325298070908,
-0.023839816451072693,
-0.04909419268369675,
-0.00583348423242569,
0.06381774693727493,
-0.02025091089308262,
-0.05896274000406265,
0.05165412649512291,
0.039022281765937805,
-0.04217037931084633,
0.005597176030278206,
0.004478695336729288,
-0.024107815697789192,
-0.027499698102474213,
0.0030639544129371643,
0.06329773366451263,
0.07152104377746582,
-0.051477644592523575,
-0.03406554460525513,
-0.06313860416412354,
-0.17339512705802917,
0.050081100314855576,
0.044925570487976074,
0.014593848027288914,
-0.04861312359571457,
0.024584701284766197,
0.02691608853638172,
0.009189784526824951,
-0.011824865825474262,
-0.016927311196923256,
0.07724114507436752,
0.04042008891701698,
0.015293438918888569,
-0.010476905852556229,
0.008965699933469296,
0.06611175835132599,
-0.000899408187251538,
-0.05326952040195465,
0.01360231451690197,
0.10504838824272156,
-0.016515005379915237,
0.03826737031340599,
0.08017075061798096,
0.006528067402541637,
0.004892098251730204,
0.009850997477769852,
0.026991793885827065,
0.049053091555833817,
0.006821144837886095,
-0.028867730870842934,
0.08733219653367996,
-0.04828585311770439,
0.0002851660829037428,
-0.15357668697834015,
0.035531990230083466,
0.027611488476395607,
0.0023327688686549664,
-0.03816725313663483,
0.0021050216164439917,
0.017473135143518448,
-0.008943631313741207,
-0.08645875006914139,
0.012109935283660889,
-0.0015335093485191464,
0.002691912464797497,
0.04788440093398094,
-0.048616454005241394,
0.007185073569417,
-0.013658329844474792,
-0.01838091015815735,
-0.012230424210429192,
0.04456021264195442,
0.011537659913301468,
-0.0014581073774024844,
-0.012928290292620659,
0.013603060506284237,
0.022442961111664772,
0.02192041650414467,
-0.015444638207554817,
0.02177548222243786,
-0.009381359443068504,
0.009241458959877491,
-0.07470415532588959,
0.00569586968049407,
0.018995752558112144,
-0.027197662740945816,
-0.0531696118414402,
-0.00804997980594635,
0.026067258790135384,
0.09493479877710342,
0.3816204369068146,
0.006717303767800331,
0.06421791017055511,
-0.027758240699768066,
-0.015167150646448135,
-0.010123798623681068,
-0.05262257158756256,
-0.06609710305929184,
-0.0640864223241806,
0.041480809450149536,
0.005677950102835894,
0.01523006521165371,
-0.0162301454693079,
0.07860201597213745,
-0.05564674735069275,
0.0048063104040920734,
0.01988033391535282,
0.005154914688318968,
-0.03131652623414993,
0.05088914558291435,
0.030874257907271385,
-0.040185485035181046,
0.008774885907769203,
0.023319542407989502,
0.013075933791697025,
0.026927059516310692,
-0.023301323875784874,
-0.019610058516263962,
0.08598658442497253,
0.030031902715563774,
0.016979340463876724,
0.013551637530326843,
0.043059781193733215,
0.005391623824834824,
-0.0030666578095406294,
-0.018806030973792076,
0.007465984672307968,
0.005359911825507879,
-0.0203582514077425,
0.029418716207146645,
0.0280938521027565,
-0.04209210351109505,
-0.024040205404162407,
0.03875768184661865,
-0.07800116389989853,
-0.060762036591768265,
0.07477526366710663,
0.059325676411390305,
0.013727250508964062,
-0.011109770275652409,
0.00005426636562333442,
-0.011369410902261734,
0.06567351520061493,
-0.016432952135801315,
0.048632364720106125,
-0.0015828973846510053,
-0.027018675580620766,
0.008782384917140007,
-0.005740433931350708,
-0.0037678354419767857,
0.03568214923143387,
-0.032655149698257446,
-0.00939719844609499,
-0.0160068329423666,
0.15314333140850067,
-0.03597680479288101,
-0.03674530237913132,
-0.005294687580317259,
-0.013490031473338604,
0.019632970914244652,
0.0124973738566041,
0.034864213317632675,
-0.026632172986865044,
-0.019095761701464653,
-0.016975784674286842,
-0.09699112921953201,
-0.048765454441308975,
0.01708620972931385,
-0.009185831993818283,
0.04106578230857849,
-0.03354618698358536,
0.045645371079444885,
0.02293703332543373,
-0.03578587621450424,
0.04827968031167984,
0.013361411169171333,
-0.03570488467812538,
-0.03401140123605728,
-0.029814092442393303,
-0.05471571907401085,
0.07809721678495407,
-0.03693106025457382,
0.00599770899862051,
-0.0020033209584653378,
0.02917996607720852,
-0.022601952776312828,
-0.006840722635388374,
-0.04549587890505791,
-0.032937925308942795,
-0.016629012301564217,
-0.01522974856197834,
0.08697705715894699,
-0.013557291589677334,
-0.02010565623641014,
0.027501661330461502,
0.07752294093370438,
0.07070144265890121,
0.004595480859279633,
-0.02320842444896698,
0.04259660840034485,
0.05110689252614975,
0.025751110166311264,
0.008282291702926159,
-0.05519815906882286,
0.009050550870597363,
-0.02307664230465889,
-0.008650305680930614,
0.019346946850419044,
0.007660091854631901,
0.07947370409965515,
0.025631513446569443,
0.019290806725621223,
-0.02566877193748951,
0.00003747119990293868,
-0.29775315523147583,
0.020200636237859726,
0.026429107412695885,
0.0017905877903103828,
0.1004040315747261,
-0.08402274549007416,
0.029087454080581665,
-0.030822552740573883,
0.00244922679848969,
-0.000755727116484195,
0.057487793266773224,
0.08747421950101852,
-0.05818264186382294,
-0.02786830998957157,
0.006375862285494804,
0.025706307962536812,
0.06130583956837654,
-0.012843490578234196,
-0.010323332622647285,
0.012799796648323536,
0.0014864846598356962,
0.009078143164515495,
-0.02297482267022133,
0.018005089834332466,
0.0851733386516571,
-0.017477866262197495,
0.10118867456912994,
-0.005423211958259344,
-0.028415394946932793,
0.026501907035708427,
-0.03914780169725418,
0.0012477831915020943,
-0.028175700455904007,
-0.09906523674726486,
-0.0496661476790905,
-0.08291765302419662,
0.0006059061270207167,
-0.06859584897756577,
-0.02045082487165928,
0.0005200803279876709,
-0.006992863956838846,
0.009796129539608955,
0.047888051718473434,
-0.02569747529923916,
-0.06946644932031631,
-0.03374404460191727,
-0.05949441343545914,
0.009494285099208355,
-0.01740987040102482,
0.03548276424407959,
0.028221456333994865,
-0.0054457117803394794,
0.025816095992922783,
-0.03675120323896408,
0.027080019935965538,
0.023038018494844437,
-0.09319108724594116,
-0.04403052106499672,
-0.0778648778796196,
-0.050094179809093475,
0.02344026230275631,
0.04395449161529541,
0.016835952177643776,
0.004427006933838129,
0.023677965626120567,
0.025699812918901443,
0.03233283385634422,
-0.04659663885831833,
0.10240063071250916,
0.02308117225766182,
-0.005736893974244595,
0.08896934986114502,
-0.01331549696624279,
0.026642432436347008,
-0.0053497785702347755,
0.031425654888153076,
-0.05810870602726936,
-0.02997763268649578,
-0.06328835338354111,
0.0009996566222980618,
-0.03897709399461746,
-0.05320330336689949,
0.07603597640991211,
0.008163134567439556,
0.028763169422745705,
0.020225586369633675,
0.03042892925441265,
-0.05559824779629707,
0.054748423397541046,
-0.0003833751252386719,
0.06039845198392868,
0.03917299211025238,
-0.032353706657886505,
-0.02790648117661476,
0.034705955535173416,
-0.0008895813953131437,
-0.26151803135871887,
0.006580177694559097,
0.0121912257745862,
0.03747474402189255,
-0.006730866618454456,
0.030652344226837158,
0.027403824031352997,
-0.030154507607221603,
-0.0534912645816803,
0.012889016419649124,
-0.02581908367574215,
0.023924589157104492,
0.036484695971012115,
-0.02231159806251526,
-0.03372207283973694,
-0.004952115472406149,
0.07685502618551254,
-0.03530418500304222,
0.014582257717847824,
0.0037732848431915045,
0.042189691215753555,
-0.03678350895643234,
0.130746990442276,
0.013628589920699596,
-0.08128827065229416,
-0.041183702647686005,
-0.024983461946249008,
-0.0011302403872832656,
-0.06608304381370544,
-0.04175909236073494,
-0.03743613883852959,
-0.0157088004052639,
0.043405719101428986,
-0.03617321699857712,
-0.006702465005218983,
0.08064938336610794,
-0.018209386616945267,
-0.01960858330130577,
0.0048509095795452595,
0.007699708454310894,
-0.028825124725699425,
-0.043934714049100876,
-0.08494845032691956,
-0.08283226191997528,
0.018166610971093178,
-0.09104178100824356,
-0.05449528992176056,
-0.029794521629810333,
0.01870473101735115,
0.007343790959566832,
-0.04712110385298729,
0.0032218422275036573,
-0.020415691658854485,
0.03160592541098595,
-0.023361744359135628,
0.08598300069570541,
-0.053610123693943024,
0.013466348871588707,
-0.0017822041409090161,
-0.023098018020391464,
0.00025392050156369805,
0.03711313381791115,
0.015874477103352547,
0.10851816087961197,
0.013555316254496574
] |
PMID:8222 | Diagnosis of infectious hepatitis by multicomponent analysis with use of field ionization mass spectrometry. | Field ionization mass spectrometry has been applied to multicomponent analysis of metabolites in human urine for the diagnosis of metabolic disorders, exemplified here by infectious hepatitis. The molecular weight profiles of carboxylic acids and of "neutral" metabolites in the urine of patients with infectious hepatitis were compared with those in urine of normals. The "neutral" metabolites showed 24 characteristic spectral differences in the range 68 to 215 atomic mass units, which provided correct diagnoses in 100% of the cases and a "diagnostic power" of unity. These results are even more encouraging than those obtained earlier with the acidic metabolites, where 11 mass numbers were found in the same mass range to be useful for correct diagnoses in 91% of the cases. The measurements were performed on urine samples preextracted on Amberlite XAD-2 columns. We used a quadrupole mass spectrometer interfaced with a multichannel analyzer for mass analyses. | Diagnosis of infectious hepatitis by multicomponent analysis with use of field ionization mass spectrometry. Field ionization mass spectrometry has been applied to multicomponent analysis of metabolites in human urine for the diagnosis of metabolic disorders, exemplified here by infectious hepatitis. The molecular weight profiles of carboxylic acids and of "neutral" metabolites in the urine of patients with infectious hepatitis were compared with those in urine of normals. The "neutral" metabolites showed 24 characteristic spectral differences in the range 68 to 215 atomic mass units, which provided correct diagnoses in 100% of the cases and a "diagnostic power" of unity. These results are even more encouraging than those obtained earlier with the acidic metabolites, where 11 mass numbers were found in the same mass range to be useful for correct diagnoses in 91% of the cases. The measurements were performed on urine samples preextracted on Amberlite XAD-2 columns. We used a quadrupole mass spectrometer interfaced with a multichannel analyzer for mass analyses. | [
-0.026932040229439735,
0.039059873670339584,
0.015262913890182972,
0.0003591101267375052,
0.054914627224206924,
-0.017307113856077194,
0.04288967698812485,
0.039338406175374985,
-0.0135140810161829,
-0.004210294224321842,
0.057863909751176834,
-0.1009933203458786,
0.012133383192121983,
0.030582280829548836,
-0.01434484776109457,
-0.036290038377046585,
0.032283492386341095,
0.010246623307466507,
0.007059331517666578,
0.0021355883218348026,
-0.008313087746500969,
0.00026200886350125074,
0.0094678346067667,
-0.047928109765052795,
0.07710981369018555,
0.06231021136045456,
0.027777716517448425,
-0.034078579396009445,
-0.07982709258794785,
-0.1871834695339203,
0.05619727075099945,
0.011366825550794601,
0.003621253650635481,
-0.05519593879580498,
0.017220033332705498,
-0.000577767554204911,
0.023987984284758568,
-0.0014889398589730263,
-0.004451911896467209,
0.0024480605497956276,
0.00025865959469228983,
0.0016022449126467109,
-0.016371220350265503,
-0.03195245563983917,
-0.04553088918328285,
-0.005508872214704752,
-0.08725613355636597,
0.03032856248319149,
0.11938271671533585,
0.06245357543230057,
-0.04395169019699097,
-0.007590057328343391,
0.024133358150720596,
0.0857233926653862,
0.011401746422052383,
-0.03903299942612648,
0.02892506681382656,
-0.015527025796473026,
-0.03696978837251663,
0.009610570967197418,
-0.006307502277195454,
0.02882986329495907,
-0.1233740746974945,
0.032920368015766144,
0.08895275741815567,
0.0017800178611651063,
-0.0407676063477993,
-0.03740163892507553,
0.04865087568759918,
0.0036208410747349262,
-0.06728137284517288,
0.008836885914206505,
-0.017187006771564484,
0.05634783208370209,
0.027240199968218803,
-0.010804886929690838,
-0.00020659182337112725,
-0.005382399540394545,
0.011187932454049587,
0.03135058656334877,
-0.043198395520448685,
0.03261753171682358,
0.012087664566934109,
0.008362358435988426,
0.02771274745464325,
-0.019811170175671577,
-0.0033440710976719856,
-0.03180428221821785,
0.006673671305179596,
0.009199903346598148,
-0.020177338272333145,
-0.012259868904948235,
-0.043031662702560425,
0.0031985421665012836,
-0.08735504001379013,
-0.0165853314101696,
-0.015961868688464165,
0.015857385471463203,
0.06079384684562683,
0.41134101152420044,
-0.008912493474781513,
-0.02993951551616192,
-0.01737000048160553,
-0.05757306143641472,
-0.010356380604207516,
-0.013232539407908916,
-0.007519732229411602,
0.00002942221544799395,
0.024548135697841644,
-0.004473565146327019,
0.056845858693122864,
0.026699692010879517,
0.029951024800539017,
-0.10543355345726013,
-0.045986153185367584,
0.013691427186131477,
0.02506321482360363,
0.03892640769481659,
-0.0033658251632004976,
0.019258083775639534,
0.03533835709095001,
-0.02735552377998829,
0.03505236282944679,
-0.027841508388519287,
0.05927528440952301,
-0.02523677796125412,
0.03954121097922325,
0.0938715860247612,
0.010533610358834267,
0.0758233517408371,
0.011417388916015625,
0.005620666313916445,
-0.033379677683115005,
-0.018159741535782814,
0.007352395448833704,
0.023860817775130272,
-0.00802522711455822,
-0.026635585352778435,
0.07809554785490036,
0.09184678643941879,
-0.07939723134040833,
-0.04086200147867203,
-0.09321997314691544,
-0.0746692568063736,
-0.08156946301460266,
0.10540247708559036,
-0.026448465883731842,
0.00000425606867793249,
-0.0032719725277274847,
-0.05846337229013443,
0.04541592299938202,
0.06813289225101471,
-0.030775276944041252,
0.003542146412655711,
0.023986676707863808,
-0.03219062089920044,
0.004413309041410685,
-0.02221008762717247,
-0.0277871061116457,
0.005618296097964048,
-0.03370243310928345,
-0.02695596031844616,
0.0024448782205581665,
0.15062808990478516,
-0.010924127884209156,
-0.015052328817546368,
-0.04647286981344223,
-0.05569888651371002,
0.005167394410818815,
-0.014146302826702595,
0.03925579413771629,
-0.04010026156902313,
-0.003750982927158475,
0.011845906265079975,
-0.004640284460037947,
-0.04699369892477989,
-0.03767675906419754,
0.05424956604838371,
0.0036774640902876854,
0.002756178844720125,
0.03132976219058037,
0.05504607409238815,
-0.030727682635188103,
0.058878958225250244,
0.06115954369306564,
-0.04276930168271065,
-0.05997460335493088,
0.004829145036637783,
0.014586524106562138,
0.03239172324538231,
0.012583085335791111,
0.024256328120827675,
-0.07199342548847198,
0.017273282632231712,
-0.031080907210707664,
0.046048153191804886,
-0.024871723726391792,
-0.00493907043710351,
-0.007847453467547894,
-0.032897211611270905,
0.07403218001127243,
0.007138575427234173,
0.07219506055116653,
0.049702320247888565,
-0.006829789839684963,
-0.011629668064415455,
-0.015215069986879826,
0.009608420543372631,
0.0043944669887423515,
0.023758651688694954,
-0.019060002639889717,
0.002271470846608281,
-0.07668241858482361,
-0.024690967053174973,
-0.008992504328489304,
-0.017842423170804977,
0.05646023899316788,
0.0025462673511356115,
0.0066646491177380085,
0.053979914635419846,
0.0042259409092366695,
-0.034692730754613876,
-0.05750991031527519,
-0.2774176299571991,
-0.020742859691381454,
0.0023074455093592405,
-0.027817081660032272,
0.04859774187207222,
-0.042652763426303864,
-0.015391521155834198,
0.0008798553026281297,
-0.008759621530771255,
0.023043107241392136,
0.05606893450021744,
0.07639668881893158,
0.002477292437106371,
-0.03838727995753288,
-0.05773898586630821,
0.056607261300086975,
0.01122054923325777,
-0.03660646826028824,
-0.0064524151384830475,
0.019565029069781303,
0.06762940436601639,
-0.038198526948690414,
0.003841062542051077,
0.0270705446600914,
0.07429692149162292,
-0.04573920741677284,
0.12218295782804489,
0.05981030687689781,
-0.03507715463638306,
-0.007117217872291803,
0.019827434793114662,
0.02590443193912506,
0.04666833579540253,
-0.0746564269065857,
-0.028790578246116638,
-0.07954683154821396,
-0.05119192972779274,
-0.02906668744981289,
-0.03024468384683132,
0.011156340129673481,
-0.045406848192214966,
-0.0308994073420763,
0.07093648612499237,
-0.09278266876935959,
-0.007032318040728569,
-0.017764655873179436,
-0.007553326431661844,
-0.018168721348047256,
0.03312614932656288,
-0.021617328748106956,
0.08783677220344543,
0.04472509026527405,
-0.03943551331758499,
-0.02349100634455681,
0.016839459538459778,
-0.017159683629870415,
-0.052059151232242584,
-0.03405222296714783,
-0.039426665753126144,
-0.032888807356357574,
-0.002317458391189575,
-0.031534966081380844,
0.03912334889173508,
0.027344083413481712,
0.0126251932233572,
0.0053572203032672405,
0.003813149407505989,
-0.012135216034948826,
0.07556241005659103,
0.022060813382267952,
0.011778457090258598,
0.10692862421274185,
-0.02008533477783203,
0.028550423681735992,
0.0657384917140007,
-0.0007926452672109008,
-0.02110818400979042,
-0.04821205884218216,
-0.06500846147537231,
0.044338203966617584,
0.05321750417351723,
-0.008273551240563393,
0.01827797293663025,
-0.039063069969415665,
0.008250025101006031,
0.03793879225850105,
0.019565945491194725,
-0.00026403481024317443,
-0.0037650049198418856,
-0.00004415902367327362,
0.021327713504433632,
0.0026815624441951513,
-0.05015290528535843,
-0.03247629478573799,
-0.037020325660705566,
0.04732907935976982,
-0.2496388852596283,
0.0007871261914260685,
-0.009664683602750301,
-0.0008051632321439683,
-0.0004621228144969791,
-0.0042851995676755905,
0.011822890490293503,
-0.0046480754390358925,
-0.022298742085695267,
-0.01798044517636299,
0.051218125969171524,
0.025980228558182716,
0.07333958894014359,
-0.023627014830708504,
-0.013268289156258106,
0.05077679455280304,
0.05295485630631447,
-0.002663607709109783,
-0.035085488110780716,
-0.00891197845339775,
-0.00203171675093472,
-0.007930304855108261,
0.18626222014427185,
-0.025520583614706993,
-0.033651307225227356,
0.019761621952056885,
-0.001173754339106381,
-0.023598380386829376,
-0.026006430387496948,
-0.01314911525696516,
0.007710711564868689,
-0.012456118129193783,
0.08183211088180542,
-0.023308884352445602,
0.026331782341003418,
0.034074559807777405,
0.019868334755301476,
0.025662560015916824,
-0.05266736447811127,
0.0404389351606369,
-0.046223483979701996,
-0.01609291136264801,
-0.039632681757211685,
-0.04338987171649933,
-0.012291986495256424,
-0.03826015070080757,
-0.029345594346523285,
-0.06046363338828087,
-0.003944110125303268,
0.0027704823296517134,
-0.031817834824323654,
-0.0017853095196187496,
0.0022597184870392084,
-0.020791184157133102,
0.013507254421710968,
0.036630090326070786,
-0.02391226217150688,
-0.018304822966456413,
0.024311307817697525,
-0.048900436609983444,
0.024812815710902214,
0.07935471087694168,
-0.05942164361476898,
0.05734752118587494,
-0.028545556589961052
] |
PMID:8223 | Reversed-phase liquid-chromatographic analysis of hemodialysate from uremic patients. | With reversed-phase high-performance liquid chromatography we effected rapid and efficient separation of metabolites present in hemodialysate fluid from uremic patients on the artificial kidney. With an aqueous sodium acetate/methanol gradient as mobile phase, more than 50 ultraviolet-absorbing constituents were resolved in a 70-min chromatogram of a 100-mul sample of dialysate. Many of the metabolites could be detected in concentrations below 0.1 mg/liter. From results of ultraviolet spectrophotometric analysis and the gas- and liquid-chromatographic properties of standards, half the observed chromatographic peaks have been characterized or identified. The method has been shown to be more than 10-fold faster than comparable separations by ion-exchange techniques. | Reversed-phase liquid-chromatographic analysis of hemodialysate from uremic patients. With reversed-phase high-performance liquid chromatography we effected rapid and efficient separation of metabolites present in hemodialysate fluid from uremic patients on the artificial kidney. With an aqueous sodium acetate/methanol gradient as mobile phase, more than 50 ultraviolet-absorbing constituents were resolved in a 70-min chromatogram of a 100-mul sample of dialysate. Many of the metabolites could be detected in concentrations below 0.1 mg/liter. From results of ultraviolet spectrophotometric analysis and the gas- and liquid-chromatographic properties of standards, half the observed chromatographic peaks have been characterized or identified. The method has been shown to be more than 10-fold faster than comparable separations by ion-exchange techniques. | [
-0.10149890184402466,
-0.00026997632812708616,
0.01212550699710846,
0.03326494246721268,
-0.009977155365049839,
-0.02448773756623268,
0.028330037370324135,
0.04102163389325142,
-0.04601410776376724,
-0.0068014031276106834,
0.013610726222395897,
-0.06914333254098892,
0.004662692546844482,
0.0025761842261999846,
-0.053334418684244156,
-0.031394973397254944,
0.06097918376326561,
0.013936528004705906,
-0.07419119030237198,
0.015455985441803932,
0.06742657721042633,
-0.025219423696398735,
-0.01220143772661686,
0.00218723202124238,
0.06907937675714493,
0.005490576382726431,
0.02763846516609192,
-0.0035739117302000523,
-0.06314630806446075,
-0.18920452892780304,
0.05986681953072548,
0.017702577635645866,
0.028867287561297417,
-0.05207408592104912,
0.009922909550368786,
0.018931999802589417,
0.001192486728541553,
0.006263189949095249,
-0.022863684222102165,
0.025280017405748367,
0.020445790141820908,
0.030817311257123947,
-0.04069190472364426,
0.018220042809844017,
0.004183460958302021,
0.0005654643755406141,
-0.03498777374625206,
0.056857798248529434,
0.10060206055641174,
0.009489916265010834,
-0.01398287434130907,
0.06806709617376328,
0.04225607216358185,
0.04576407000422478,
0.00338339083828032,
-0.018236352130770683,
0.048973970115184784,
-0.013495719991624355,
0.029499024152755737,
0.06370172649621964,
-0.0215668436139822,
0.023604674264788628,
-0.12810003757476807,
0.05675264447927475,
0.011198126710951328,
-0.0018666606629267335,
-0.005804033484309912,
-0.046508077532052994,
0.04105934500694275,
-0.05875284969806671,
-0.10803478211164474,
-0.000664678227622062,
-0.005651672836393118,
0.07537476718425751,
0.020476415753364563,
-0.029114793986082077,
-0.013781491667032242,
-0.03940834850072861,
0.0433587022125721,
0.015295063145458698,
-0.004199327435344458,
-0.01014156173914671,
0.006461555603891611,
-0.006640834733843803,
0.019929533824324608,
-0.018911033868789673,
0.02395397424697876,
-0.010359621606767178,
0.040899183601140976,
0.0040377043187618256,
0.007773414254188538,
-0.029177505522966385,
0.0006786073790863156,
0.029406366869807243,
-0.028636354953050613,
-0.023219088092446327,
-0.04384112358093262,
0.021216344088315964,
0.04964017868041992,
0.424555242061615,
-0.011395116336643696,
0.02830459550023079,
0.0062058293260633945,
-0.027762243524193764,
0.027376027777791023,
-0.022824913263320923,
-0.04153681546449661,
-0.006797243840992451,
0.019205259159207344,
0.0034363409504294395,
0.04064461216330528,
0.010423601604998112,
0.10331250727176666,
-0.009142713621258736,
-0.053449489176273346,
0.01940770633518696,
0.03162388503551483,
0.0016774667892605066,
-0.021792815998196602,
0.04954370856285095,
0.018685750663280487,
0.01449967734515667,
0.004202655050903559,
-0.017074909061193466,
0.022137422114610672,
-0.002059642691165209,
0.028150424361228943,
0.05985754728317261,
0.011961723677814007,
0.013074364513158798,
-0.007959644310176373,
0.025684917345643044,
-0.0018253797898069024,
0.0034921132028102875,
-0.030399497598409653,
0.018439671024680138,
0.0429525263607502,
-0.051559656858444214,
0.06519387662410736,
0.04453178495168686,
-0.015800217166543007,
-0.027006099000573158,
-0.021707192063331604,
-0.06800346076488495,
-0.047452740371227264,
0.09081291407346725,
0.05155379697680473,
0.017979027703404427,
-0.04023090749979019,
-0.08053464442491531,
0.03756082057952881,
0.053700532764196396,
-0.08619224280118942,
0.007954412139952183,
-0.002430844819173217,
-0.02703254297375679,
-0.00590143259614706,
0.03500894829630852,
0.010373376309871674,
0.02983838878571987,
-0.04878053814172745,
-0.04769933596253395,
-0.0344846248626709,
0.08258049935102463,
-0.02053077332675457,
-0.03803107142448425,
0.009941736236214638,
0.005679354537278414,
0.007404287811368704,
0.013546823523938656,
0.018598303198814392,
0.0133502297103405,
-0.027430284768342972,
-0.02031962014734745,
-0.03214650601148605,
0.0016230068868026137,
0.009319650940597057,
-0.06011853739619255,
-0.00960507895797491,
0.010410928167402744,
0.04038587585091591,
0.039412785321474075,
-0.07431042939424515,
0.04748360067605972,
0.04874114692211151,
-0.0528259351849556,
-0.05507112294435501,
-0.013926493003964424,
-0.034874312579631805,
0.06627313792705536,
0.013210096396505833,
0.006835191044956446,
-0.016708092764019966,
0.012764953076839447,
0.009546469897031784,
0.0034964634105563164,
-0.06735315918922424,
-0.06645990163087845,
-0.051767490804195404,
-0.03458044305443764,
0.07817641645669937,
-0.01004624180495739,
0.01781477965414524,
0.04425475746393204,
0.06379272043704987,
0.04570210725069046,
-0.009756801649928093,
0.012240415439009666,
0.041678354144096375,
-0.0015485914191231132,
-0.01134476251900196,
0.031288471072912216,
-0.034021440893411636,
0.033356960862874985,
-0.029534751549363136,
-0.034567151218652725,
0.03348921611905098,
0.027951594442129135,
0.05709848552942276,
0.02065294049680233,
0.029536083340644836,
-0.0032141630072146654,
-0.03746455907821655,
-0.2999046742916107,
-0.02356116659939289,
0.02467089146375656,
0.010883135721087456,
0.09376078099012375,
-0.04664633050560951,
-0.03412666544318199,
-0.008558617904782295,
0.007869581691920757,
-0.008059852756559849,
0.07533033192157745,
0.09102039039134979,
-0.01424714270979166,
-0.026086295023560524,
-0.008819007314741611,
0.009954359382390976,
0.05505360662937164,
-0.02066963165998459,
0.026348179206252098,
0.013611744157969952,
0.061988335102796555,
-0.0070984517224133015,
-0.014270023442804813,
0.03935282677412033,
0.04898730665445328,
0.01936044543981552,
0.10404796153306961,
-0.06031545251607895,
-0.015560965053737164,
-0.049462828785181046,
0.026690354570746422,
-0.03689586743712425,
-0.005079036112874746,
-0.0901321992278099,
-0.047969501465559006,
-0.0504005141556263,
0.01562798023223877,
-0.05418010801076889,
-0.02101084776222706,
-0.048162560909986496,
-0.059245191514492035,
0.008089450187981129,
0.055324140936136246,
-0.03278209641575813,
-0.05677548050880432,
0.013014528900384903,
-0.03363741189241409,
-0.0035940920934081078,
-0.03311549872159958,
-0.007791848387569189,
0.021956589072942734,
0.010694093070924282,
0.027691936120390892,
0.00727092893794179,
0.031516481190919876,
0.013213946484029293,
-0.0827675387263298,
-0.05107979476451874,
-0.058291953057050705,
-0.063649021089077,
0.0093462523072958,
0.04958874359726906,
0.030767397955060005,
0.009707416407763958,
0.004625733941793442,
0.026064053177833557,
0.04440698027610779,
-0.010501696728169918,
0.1022660881280899,
-0.016254736110568047,
-0.014406696893274784,
0.09349686652421951,
-0.06057154759764671,
0.07359005510807037,
0.018204856663942337,
0.08175937086343765,
-0.027975454926490784,
-0.06991812586784363,
-0.06157904118299484,
0.02458365447819233,
0.019596150144934654,
-0.027071965858340263,
0.02341344580054283,
-0.0312594510614872,
0.04045094549655914,
0.02776096574962139,
-0.010774502530694008,
-0.020216623321175575,
-0.012425435706973076,
0.04054166376590729,
0.03883875533938408,
0.014923682436347008,
-0.08561137318611145,
-0.044864214956760406,
-0.01201270055025816,
0.017353422939777374,
-0.28547203540802,
0.00029214410460554063,
-0.022991951555013657,
0.0006677587516605854,
0.0018944162875413895,
-0.003513023257255554,
0.021638715639710426,
-0.045553408563137054,
0.018588891252875328,
-0.0052780830301344395,
0.01570308580994606,
0.008949194103479385,
0.05984923616051674,
0.0214527640491724,
0.017514288425445557,
0.01736895740032196,
0.0686631128191948,
0.03154079616069794,
0.017833735793828964,
-0.010216765105724335,
0.020596731454133987,
-0.03387005627155304,
0.14305122196674347,
0.029804902151226997,
-0.06833577901124954,
0.021662794053554535,
-0.01599327102303505,
0.0018213383154943585,
-0.13154561817646027,
0.003337560687214136,
0.008054295554757118,
0.011322762817144394,
0.06840148568153381,
0.009881168603897095,
-0.02405511401593685,
0.02010900154709816,
-0.025656916201114655,
-0.002898036502301693,
-0.029427532106637955,
-0.003749302588403225,
-0.05092360079288483,
-0.02452472783625126,
-0.045752622187137604,
-0.06510941684246063,
0.043133463710546494,
-0.04303994029760361,
-0.012970809824764729,
-0.0778244361281395,
0.03813065588474274,
-0.008336152881383896,
-0.051919933408498764,
-0.01634264551103115,
-0.01100208144634962,
-0.002458582166582346,
-0.004829723387956619,
0.0050629680044949055,
-0.03629197180271149,
-0.015463807620108128,
0.030666610226035118,
-0.031440433114767075,
0.010288373567163944,
0.023720914497971535,
-0.0050615896470844746,
0.025650421157479286,
-0.016149291768670082
] |
PMID:8226 | Human leukocyte acid hydrolases: characterization of eleven lysosomal enzymes and study of reaction conditions for their automated analysis. | The optimal reaction conditions and kinetic properties of eleven leukocyte acid hydrolases determined with the use of fluorigenic derivatives of 4-methyl-umbelliferone are described. The enzymes studied were acid phosphatase, aryl sulfatase, alpha- and beta-glucosidase, alpha- and beta-galactosidase, alpha-mannosidase, N-acetyl-beta-glucosaminidase, N-acetyl-beta-galactosaminidase, beta-glucuronidase and alpha-fucosidase. More than 90% of the activity of each enzyme was released into a 27,000 X g supernatant by a double sonication procedure employing 0.9% sodium chloride and 0.1% Triton X-100. The Km values obtained were similar to those previously reported for chromogenic subtrates. A single Km value could not be derived for beta-galactosidase because its double reciprocal plot was not linear. All enzymes could be measured with less than 10 mug of protein within 15 min. Activators and inhibitors studied included the chloride salts of Na+, K+, Zn2+, Ca2+, Mg2+, Hg2+, and Fe2+ as well as p-chloromercuriphenysulfonate, glutathione, BAL, EDTA, EGTA, Triton X-100 and sodium taurocholate. The reaction conditions described in this report can be used for the diagnosis of various lysosomal storage diseases and should facilitate the development of automated procedures for the analysis of these eleven enzyme activities with small quantities of blood. | Human leukocyte acid hydrolases: characterization of eleven lysosomal enzymes and study of reaction conditions for their automated analysis. The optimal reaction conditions and kinetic properties of eleven leukocyte acid hydrolases determined with the use of fluorigenic derivatives of 4-methyl-umbelliferone are described. The enzymes studied were acid phosphatase, aryl sulfatase, alpha- and beta-glucosidase, alpha- and beta-galactosidase, alpha-mannosidase, N-acetyl-beta-glucosaminidase, N-acetyl-beta-galactosaminidase, beta-glucuronidase and alpha-fucosidase. More than 90% of the activity of each enzyme was released into a 27,000 X g supernatant by a double sonication procedure employing 0.9% sodium chloride and 0.1% Triton X-100. The Km values obtained were similar to those previously reported for chromogenic subtrates. A single Km value could not be derived for beta-galactosidase because its double reciprocal plot was not linear. All enzymes could be measured with less than 10 mug of protein within 15 min. Activators and inhibitors studied included the chloride salts of Na+, K+, Zn2+, Ca2+, Mg2+, Hg2+, and Fe2+ as well as p-chloromercuriphenysulfonate, glutathione, BAL, EDTA, EGTA, Triton X-100 and sodium taurocholate. The reaction conditions described in this report can be used for the diagnosis of various lysosomal storage diseases and should facilitate the development of automated procedures for the analysis of these eleven enzyme activities with small quantities of blood. | [
-0.08074706047773361,
-0.03131391108036041,
-0.04175068810582161,
0.04740574210882187,
-0.014709305949509144,
-0.03878038004040718,
0.04487036541104317,
0.08088363707065582,
-0.013555056415498257,
-0.05544459819793701,
0.031349409371614456,
-0.06059902533888817,
-0.07021374255418777,
-0.006057451944798231,
-0.014364016242325306,
-0.030773190781474113,
0.02427620440721512,
0.0027425368316471577,
-0.08502064645290375,
-0.004386899061501026,
0.03859433904290199,
0.023779671639204025,
0.0008671050891280174,
-0.025594552978873253,
0.03113974817097187,
0.030380859971046448,
0.009226536378264427,
-0.019961131736636162,
-0.06825575977563858,
-0.1624222993850708,
0.016658669337630272,
-0.06353604793548584,
0.02876715548336506,
-0.05353889241814613,
0.017840929329395294,
0.026980658993124962,
-0.00360222440212965,
-0.019279398024082184,
0.008708851411938667,
0.01959659345448017,
0.02402217499911785,
0.009179080836474895,
0.019606878980994225,
0.02025839500129223,
0.004309454932808876,
-0.01954883709549904,
-0.06842222809791565,
-0.009760049171745777,
0.018282314762473106,
0.05182427912950516,
-0.005246298853307962,
0.011297364719212055,
0.005191105883568525,
0.04013792425394058,
-0.029992250725626945,
-0.05054967850446701,
0.017441755160689354,
0.025273295119404793,
0.01053162943571806,
0.011538960970938206,
0.0377654954791069,
-0.024624181911349297,
-0.1397731453180313,
0.037358853965997696,
0.011594004929065704,
0.019759194925427437,
-0.02053934894502163,
-0.051680926233530045,
0.046441830694675446,
0.08600831776857376,
-0.07066286355257034,
0.05634578317403793,
0.03610868379473686,
0.03605198860168457,
-0.007890427485108376,
0.02065226249396801,
-0.015723710879683495,
-0.007638674695044756,
0.057595428079366684,
0.034927234053611755,
0.015849465504288673,
0.008984273299574852,
0.0365312360227108,
0.015156213194131851,
0.033649615943431854,
0.047882359474897385,
-0.004216243978589773,
0.006503727752715349,
0.04671141505241394,
0.029498519375920296,
-0.023626094684004784,
-0.02272774465382099,
-0.00012565999350044876,
0.039266087114810944,
-0.06994453817605972,
0.05922902002930641,
0.013846294954419136,
0.02070840820670128,
0.024078333750367165,
0.41659581661224365,
-0.0031435710843652487,
0.001273282803595066,
-0.021174084395170212,
-0.027954917401075363,
-0.015074402093887329,
-0.03450577333569527,
-0.018922235816717148,
-0.0510624498128891,
-0.015908844769001007,
-0.01757684536278248,
0.05323023721575737,
0.008417217060923576,
0.04304727166891098,
-0.05518258735537529,
-0.029680702835321426,
-0.010952345095574856,
0.055104050785303116,
-0.04797608405351639,
-0.04915350303053856,
0.10751162469387054,
-0.017384707927703857,
-0.07112100720405579,
0.02049098163843155,
-0.04386301338672638,
0.08523280918598175,
0.04503576457500458,
0.03743830695748329,
0.10764147341251373,
0.07183580845594406,
0.015509660355746746,
0.03771758824586868,
0.0705413892865181,
-0.034714095294475555,
0.014551675878465176,
-0.00762691767886281,
-0.04250120744109154,
0.04374956712126732,
-0.0025043480563908815,
0.024511417374014854,
0.018165213987231255,
-0.004245868418365717,
-0.035626377910375595,
-0.06180810555815697,
-0.0504840649664402,
-0.015364053659141064,
0.10356827080249786,
-0.021116264164447784,
-0.02482854761183262,
-0.042185623198747635,
0.004455004818737507,
0.05601673573255539,
0.042305514216423035,
-0.027291487902402878,
0.05476708337664604,
-0.018488578498363495,
0.0027425591833889484,
-0.0021788000594824553,
-0.03099215216934681,
0.006226795259863138,
-0.003601923817768693,
-0.05409699305891991,
-0.00997795071452856,
0.010467651300132275,
0.08066269010305405,
0.0005999504355713725,
-0.045005302876234055,
-0.0249314121901989,
-0.017701292410492897,
-0.013382615521550179,
-0.02554815262556076,
0.014891814440488815,
-0.018465114757418633,
-0.028157973662018776,
-0.04944504797458649,
-0.03724271059036255,
0.027831941843032837,
0.012272000312805176,
0.05973351001739502,
-0.01758638583123684,
0.0388590544462204,
0.015658698976039886,
0.00904217641800642,
-0.06401782482862473,
0.015152900479733944,
0.07720278203487396,
-0.051789794117212296,
0.021824359893798828,
0.014690952375531197,
0.0875222459435463,
0.02811308391392231,
-0.03884022682905197,
-0.015197457745671272,
-0.09010866284370422,
-0.040579307824373245,
0.0009064273908734322,
-0.024899356067180634,
-0.026312533766031265,
-0.03763118013739586,
0.02014876715838909,
0.008717483840882778,
0.0912390649318695,
0.050169315189123154,
0.03900067135691643,
0.06958270817995071,
0.053087301552295685,
0.0346478670835495,
-0.042871326208114624,
0.045956872403621674,
0.037565816193819046,
0.015411149710416794,
-0.020249981433153152,
0.015322285704314709,
-0.0992199182510376,
0.011716899462044239,
-0.06472163647413254,
0.022954626008868217,
-0.008973383344709873,
-0.01848483644425869,
0.03252256289124489,
-0.03260570392012596,
0.005166908260434866,
-0.027804356068372726,
-0.10949092358350754,
-0.28463906049728394,
-0.0651872456073761,
-0.0022725090384483337,
-0.008507802151143551,
0.03825930505990982,
-0.0228767991065979,
-0.00275624031201005,
-0.02334703505039215,
0.02505463920533657,
-0.05493442714214325,
0.04663857817649841,
0.04867957904934883,
-0.028518203645944595,
-0.07635769248008728,
-0.0025428342632949352,
0.05135791003704071,
0.007678491063416004,
-0.049881644546985626,
0.04396878182888031,
0.022847959771752357,
0.052800193428993225,
-0.06555663049221039,
0.05500391498208046,
0.00048463387065567076,
0.05620231106877327,
-0.005991525016725063,
0.0912259891629219,
-0.042938023805618286,
0.009367280639708042,
-0.019022228196263313,
0.04617088660597801,
0.043578363955020905,
0.0019953190349042416,
-0.05217853933572769,
-0.02698317915201187,
-0.019022969529032707,
-0.0036638735327869654,
-0.051491014659404755,
0.002120130928233266,
-0.010233206674456596,
-0.05048098415136337,
-0.0031154826283454895,
-0.0011843417305499315,
-0.06741724163293839,
-0.011904407292604446,
0.001592305488884449,
0.03634104132652283,
0.03529170900583267,
-0.009891417808830738,
-0.03511366248130798,
0.004232955630868673,
-0.033888667821884155,
0.03217007592320442,
-0.022580144926905632,
-0.06420593708753586,
0.015485207550227642,
-0.027122557163238525,
0.021305566653609276,
-0.06160588935017586,
-0.014773049391806126,
-0.029534893110394478,
-0.014963806606829166,
-0.013308336958289146,
0.060626666992902756,
0.030702605843544006,
-0.016355061903595924,
0.03174431622028351,
-0.042090386152267456,
0.023897377774119377,
-0.031177720054984093,
0.0204006340354681,
0.10127440840005875,
-0.039874471724033356,
0.02057141251862049,
0.024358296766877174,
0.00923831108957529,
-0.026064960286021233,
-0.04485156387090683,
-0.08760524541139603,
0.026466412469744682,
0.016189999878406525,
-0.034487102180719376,
0.047620780766010284,
-0.0448075532913208,
0.02900906465947628,
0.04492130130529404,
0.04549247398972511,
0.011258254759013653,
-0.051908403635025024,
-0.004872769117355347,
0.01685340702533722,
-0.0017571881180629134,
-0.020752524957060814,
-0.02092175930738449,
-0.005382590461522341,
0.01282186433672905,
-0.2675379812717438,
-0.004806347656995058,
0.02475447580218315,
0.010726051405072212,
-0.014420680701732635,
0.0033728587441146374,
0.04897288233041763,
-0.06991937756538391,
-0.010700876824557781,
0.04997672140598297,
0.06877057999372482,
0.04052690044045448,
0.10410954803228378,
-0.008091306313872337,
0.003471237374469638,
0.009851773269474506,
0.051496949046850204,
-0.0061280992813408375,
0.043175630271434784,
-0.03599241375923157,
0.0355500653386116,
-0.029540186747908592,
0.16855651140213013,
0.019091928377747536,
0.00925444345921278,
0.10018151253461838,
-0.00822178553789854,
0.0006895361584611237,
-0.056985072791576385,
0.012672819197177887,
0.022667890414595604,
0.03262905776500702,
0.03681057691574097,
-0.01779257506132126,
-0.04409075900912285,
0.05498366057872772,
-0.047275420278310776,
-0.005141222849488258,
-0.04104385897517204,
0.06399411708116531,
0.022427445277571678,
-0.02084267884492874,
-0.07206865400075912,
-0.01615051180124283,
0.0373375341296196,
-0.029861031100153923,
-0.009470744989812374,
-0.03746375814080238,
-0.014054562896490097,
-0.04913744330406189,
-0.037468332797288895,
-0.07066002488136292,
0.006483321078121662,
0.019825683906674385,
-0.008032698184251785,
0.002983466489240527,
-0.028887184336781502,
0.009072540327906609,
-0.028268037363886833,
-0.041445933282375336,
0.020261719822883606,
0.007356797810643911,
-0.0045957681722939014,
0.07921986281871796,
-0.0067748092114925385
] |
PMID:8227 | Oestrogen induced hypertriglyceridaemia: role of the adrenal cortex. | The role of the adrenal cortex in the pathogenesis of hypertriglyceridaemia associated with the intake of oral contraceptive agents containing oestrogen has been investigated in rats. Bilateral adrenalectomy reduced the activity of hepatic enzymes regulating lipogenesis (acetyl CoA carboxylase, fatty acid synthetase) and decreased plasma triglyceride concentrations. On the other hand, the administration of high dosage corticosterone induced the activity of hepatic enzymes with consequent elevation in serum triglyceride levels. In animals with intact adrenals the administration of oestradiol: (a) raised plasma triglyceride levels, (b) enhanced the activity of hepatic enzymes, and (c) increased the adrenal cortex:body weight ratio. The effects (a) and (b) were not observed when both adrenals were removed prior to oestrogen therapy. High dosage corticosterone replacement was found to be essential for the oestradiol to produce its effects on hepatic enzymes and plasma triglyceride levels. The results suggest a regulatory role for the adrenal cortex in the homeostasis of plasma triglyceride concentration and that the hypertriglyceridaemia induced by the oestrogen containing preparations might be secondary to alterations in adrenocortical function. | Oestrogen induced hypertriglyceridaemia: role of the adrenal cortex. The role of the adrenal cortex in the pathogenesis of hypertriglyceridaemia associated with the intake of oral contraceptive agents containing oestrogen has been investigated in rats. Bilateral adrenalectomy reduced the activity of hepatic enzymes regulating lipogenesis (acetyl CoA carboxylase, fatty acid synthetase) and decreased plasma triglyceride concentrations. On the other hand, the administration of high dosage corticosterone induced the activity of hepatic enzymes with consequent elevation in serum triglyceride levels. In animals with intact adrenals the administration of oestradiol: (a) raised plasma triglyceride levels, (b) enhanced the activity of hepatic enzymes, and (c) increased the adrenal cortex:body weight ratio. The effects (a) and (b) were not observed when both adrenals were removed prior to oestrogen therapy. High dosage corticosterone replacement was found to be essential for the oestradiol to produce its effects on hepatic enzymes and plasma triglyceride levels. The results suggest a regulatory role for the adrenal cortex in the homeostasis of plasma triglyceride concentration and that the hypertriglyceridaemia induced by the oestrogen containing preparations might be secondary to alterations in adrenocortical function. | [
0.03496238961815834,
-0.003930241335183382,
0.012488218024373055,
0.029887190088629723,
0.03801338002085686,
-0.06603830307722092,
0.05670025944709778,
0.03791174665093422,
-0.06238918378949165,
-0.05446233972907066,
0.018356842920184135,
-0.060440752655267715,
0.0378328412771225,
0.04190152883529663,
-0.04496930539608002,
-0.01676255837082863,
0.024828320369124413,
0.016854912042617798,
-0.0641072690486908,
0.017774930223822594,
0.004739780444651842,
0.008445053361356258,
0.0033304186072200537,
0.04394783824682236,
-0.01737034320831299,
-0.004082473926246166,
-0.024722594767808914,
-0.03782251104712486,
-0.11118719726800919,
-0.17404960095882416,
0.03171583637595177,
0.013694723136723042,
0.016643211245536804,
-0.04418685659766197,
0.01899164915084839,
-0.007010557223111391,
0.003215814707800746,
0.0420905239880085,
0.0515899732708931,
0.05018176883459091,
-0.003480629762634635,
0.024094494059681892,
0.003917076624929905,
0.03562714159488678,
0.05416101589798927,
0.020168690010905266,
-0.013575228862464428,
0.05001562833786011,
0.06714124232530594,
0.04025398939847946,
0.01916331984102726,
0.00780954584479332,
0.004605295602232218,
0.07855333387851715,
0.04261889308691025,
-0.04077602177858353,
-0.027533739805221558,
0.06535666435956955,
-0.03552059084177017,
0.06569387018680573,
0.0017391425790265203,
0.012178759090602398,
-0.16238288581371307,
0.054709140211343765,
0.03904452547430992,
-0.015189909376204014,
-0.007198118604719639,
-0.024101916700601578,
0.05153316259384155,
0.04829131066799164,
-0.07356180995702744,
0.05870148167014122,
-0.017598606646060944,
-0.014473015442490578,
0.0325184240937233,
-0.014651795849204063,
-0.007857903838157654,
-0.03445646911859512,
-0.003979250323027372,
0.0010385906789451838,
-0.017774134874343872,
0.017246365547180176,
0.012624431401491165,
0.015298370271921158,
-0.006850517354905605,
-0.0634889155626297,
-0.018758824095129967,
-0.04975336790084839,
0.002391914837062359,
0.0032444654498249292,
-0.03927652910351753,
-0.041555363684892654,
-0.035709451884031296,
0.009259737096726894,
-0.057677313685417175,
0.02865457907319069,
-0.031255386769771576,
-0.059996142983436584,
-0.03157799318432808,
0.3710767328739166,
0.0017535305814817548,
0.021607007831335068,
-0.04947500303387642,
0.00931825116276741,
-0.015199346467852592,
0.015048341825604439,
-0.0007365986821241677,
-0.06813812255859375,
-0.045040249824523926,
0.03798491507768631,
0.03871794417500496,
-0.005770151969045401,
-0.023593058809638023,
-0.04129504784941673,
-0.012776875868439674,
-0.03945998474955559,
0.04832734540104866,
0.03992822393774986,
-0.04948343336582184,
0.046768542379140854,
-0.05151567980647087,
-0.0026122210547327995,
0.025071358308196068,
0.01801048405468464,
0.027233198285102844,
0.022267641499638557,
0.05091692507266998,
0.10540612041950226,
0.03200060501694679,
-0.0024973666295409203,
0.016997873783111572,
0.03319146856665611,
-0.043198104947805405,
0.031143980100750923,
-0.010964143089950085,
-0.010614815168082714,
0.052634820342063904,
-0.04303910955786705,
0.02995515987277031,
0.0039106691256165504,
-0.03791538625955582,
-0.04206645116209984,
-0.04031181335449219,
-0.05870450288057327,
0.05292891710996628,
0.13530153036117554,
0.07053417712450027,
0.01980840228497982,
-0.007290883921086788,
0.027899153530597687,
0.05120714008808136,
0.03965875506401062,
-0.04038938879966736,
0.06454633176326752,
-0.08279567211866379,
0.011359240859746933,
-0.02054496295750141,
-0.024167407304048538,
0.02560674212872982,
-0.03849012032151222,
-0.05951370298862457,
-0.04086810350418091,
-0.017615817487239838,
0.014413335360586643,
0.07626712322235107,
0.0012533189728856087,
-0.008534339256584644,
0.018701480701565742,
-0.005333702079951763,
0.05916211009025574,
0.007251600734889507,
-0.017888132482767105,
-0.06229311227798462,
-0.013100266456604004,
-0.0353940948843956,
-0.003606254467740655,
0.04252326861023903,
0.05604388192296028,
0.0031972285360097885,
-0.04387325420975685,
0.03236221894621849,
-0.044577110558748245,
-0.016067763790488243,
0.001923966221511364,
-0.011867929250001907,
-0.02761502005159855,
-0.008338282816112041,
-0.048551496118307114,
-0.04511164501309395,
0.028354190289974213,
-0.054969675838947296,
0.06512946635484695,
-0.07769885659217834,
-0.02929362840950489,
-0.049695976078510284,
-0.06976097822189331,
0.0027066573966294527,
0.08070489019155502,
-0.019382838159799576,
-0.021472947672009468,
0.07372979819774628,
-0.0013696717796847224,
0.06634534895420074,
0.08398403227329254,
-0.009978673420846462,
0.012878896668553352,
0.024703428149223328,
-0.05364387109875679,
0.04328092560172081,
0.014630783349275589,
0.028987500816583633,
0.02005738951265812,
0.016009461134672165,
0.04082005098462105,
-0.028120659291744232,
0.08672637492418289,
-0.01728750765323639,
-0.006598899606615305,
0.06984695047140121,
0.03478538990020752,
0.07010152190923691,
-0.005689214915037155,
0.046579912304878235,
-0.33711472153663635,
-0.028749359771609306,
0.008905594237148762,
-0.022587725892663002,
-0.030293922871351242,
-0.0825079008936882,
-0.004694072529673576,
-0.0445253923535347,
-0.04398515820503235,
0.00014673714758828282,
0.03378387540578842,
0.02374928444623947,
-0.013450254686176777,
-0.03413521870970726,
-0.049649227410554886,
0.02130124531686306,
0.016510244458913803,
-0.0588826984167099,
0.033235229551792145,
0.006851665675640106,
-0.02121989242732525,
-0.03992241993546486,
-0.008645055815577507,
-0.02312612533569336,
0.12533341348171234,
-0.028232941403985023,
0.07651804387569427,
0.010235155001282692,
-0.03780023008584976,
-0.01919068768620491,
0.01621793769299984,
0.023456357419490814,
0.02170524001121521,
-0.10682100057601929,
-0.0021490128710865974,
-0.07599515467882156,
0.0354597233235836,
-0.04232298210263252,
0.0010941458167508245,
0.0035885011311620474,
-0.06384201347827911,
-0.02657722495496273,
-0.04601937532424927,
-0.014379709959030151,
-0.06928449869155884,
-0.026005178689956665,
0.006792476400732994,
0.061489537358284,
0.007489900570362806,
0.018081147223711014,
0.04128092899918556,
0.01170094683766365,
-0.013623866252601147,
0.006759574171155691,
0.019270818680524826,
-0.007058712188154459,
-0.07188025861978531,
-0.02853209152817726,
-0.07028837502002716,
0.007938556373119354,
-0.037181008607149124,
0.00395121518522501,
-0.007848941721022129,
-0.010785741731524467,
-0.00018029693455900997,
0.03380568325519562,
0.007780320942401886,
-0.010842565447092056,
0.020535573363304138,
0.07677111774682999,
-0.0013870418770238757,
0.09464401751756668,
-0.02323971688747406,
-0.02017991989850998,
0.10258373618125916,
0.019197890534996986,
-0.03617788478732109,
-0.03041856549680233,
-0.07404713332653046,
0.01330968365073204,
-0.016159208491444588,
-0.02988828346133232,
0.059629011899232864,
-0.014724383130669594,
0.04441709816455841,
-0.06392258405685425,
-0.009657506830990314,
-0.00680106645449996,
0.007959960028529167,
0.010022626258432865,
-0.020694227889180183,
0.02095802314579487,
-0.03149767592549324,
-0.03226160630583763,
0.040759824216365814,
0.028344664722681046,
-0.2651907503604889,
0.0005640409071929753,
0.01921972632408142,
-0.01394481398165226,
0.00969564076513052,
0.019324814900755882,
-0.04344075173139572,
0.029966751113533974,
-0.04237338528037071,
-0.00487373536452651,
-0.015294218435883522,
0.0381847582757473,
0.07584688812494278,
0.03751430660486221,
-0.06625112146139145,
-0.007223223801702261,
0.014144349843263626,
0.017102032899856567,
0.013995060697197914,
-0.020859381183981895,
-0.011080644093453884,
0.028700245544314384,
0.1072954460978508,
0.010809197090566158,
-0.05844490975141525,
0.008848113007843494,
0.006961124017834663,
0.05226249620318413,
0.008928097784519196,
0.011932904832065105,
0.008109353482723236,
-0.010314349085092545,
0.031706035137176514,
0.05264699459075928,
-0.015496999025344849,
0.13339565694332123,
-0.04815696179866791,
0.021069714799523354,
0.056019023060798645,
0.002249262062832713,
0.020595168694853783,
-0.008170787245035172,
-0.035839926451444626,
-0.045370664447546005,
0.025131933391094208,
-0.04694521427154541,
-0.04426297917962074,
0.0175301656126976,
0.01913554221391678,
0.02156449668109417,
-0.004325744230300188,
-0.026280993595719337,
-0.0181928388774395,
0.07766401022672653,
-0.028830643743276596,
-0.04383910074830055,
-0.025867119431495667,
0.0085016880184412,
0.00022459447791334242,
-0.0040662516839802265,
0.0014207207132130861,
0.004679492674767971,
-0.027858499437570572,
0.09183333814144135,
0.03653275594115257
] |
PMID:8228 | Increased survival times of New Zealand hybrid mice immunosuppressed by graft-versus-host reactions. | New Zealand mice develop autoimmune disease usually accompanied by glomerulonephritis. A graft-versus-host reaction was induced in New Zealand Black X New Zealand White F1 hybrid mice by administration of New Zealand White spleen cells. The mice so treated had diminished antibody responses to both an exogenous antigen (sheep red blood cells) and an endogenous antigen (native DNA). They had much less glomerulonephritis and increased survival times compared to unmanipulated controls, apparently due to immunosuppression. Similar hybrid mice treated with high doses of cyclophosphamide (70mg/kg/week) were more immunosuppressed than mice with graft-versus-host reactions and had even greater survival times. | Increased survival times of New Zealand hybrid mice immunosuppressed by graft-versus-host reactions. New Zealand mice develop autoimmune disease usually accompanied by glomerulonephritis. A graft-versus-host reaction was induced in New Zealand Black X New Zealand White F1 hybrid mice by administration of New Zealand White spleen cells. The mice so treated had diminished antibody responses to both an exogenous antigen (sheep red blood cells) and an endogenous antigen (native DNA). They had much less glomerulonephritis and increased survival times compared to unmanipulated controls, apparently due to immunosuppression. Similar hybrid mice treated with high doses of cyclophosphamide (70mg/kg/week) were more immunosuppressed than mice with graft-versus-host reactions and had even greater survival times. | [
-0.043154697865247726,
0.029315732419490814,
0.009685210883617401,
0.04040073975920677,
0.02293361723423004,
-0.018734894692897797,
0.038177240639925,
0.11053404211997986,
-0.052680399268865585,
0.00036856200313195586,
0.023746002465486526,
-0.020760947838425636,
0.016784140840172768,
0.0006912784301675856,
-0.0353550985455513,
0.025112297385931015,
-0.050048209726810455,
-0.012475747615098953,
-0.09456801414489746,
0.03203878179192543,
-0.0693279504776001,
-0.03509470820426941,
0.014171983115375042,
-0.005241680424660444,
0.05657380819320679,
0.010943698696792126,
0.023872967809438705,
0.05436237156391144,
-0.009001821279525757,
-0.1798425167798996,
-0.04082679748535156,
-0.023865515366196632,
-0.041965302079916,
-0.039450354874134064,
-0.01824495568871498,
-0.033058296889066696,
-0.003957461100071669,
-0.0021695292089134455,
0.009271955117583275,
0.05579310655593872,
0.050533540546894073,
0.022739920765161514,
-0.009099546819925308,
0.05667376518249512,
0.03793488070368767,
0.02178584784269333,
-0.060297511518001556,
-0.03913592919707298,
0.019034355878829956,
0.02500729449093342,
0.03659454733133316,
-0.025929998606443405,
0.038007527589797974,
0.018270650878548622,
-0.015588738024234772,
0.0169670507311821,
0.013470646925270557,
-0.004863678012043238,
0.025971129536628723,
-0.015196780674159527,
-0.00869979988783598,
0.018563710153102875,
-0.11109261214733124,
0.09391510486602783,
0.07763709872961044,
0.00640585832297802,
-0.04285852611064911,
-0.05342681333422661,
0.005666790995746851,
0.0019772611558437347,
-0.07580465823411942,
0.03507883846759796,
0.016263579949736595,
0.0009165166411548853,
-0.032851047813892365,
0.0055088503286242485,
-0.012648393400013447,
-0.026265351101756096,
0.08255160599946976,
0.006265316624194384,
0.050586771219968796,
-0.014772355556488037,
-0.02721940539777279,
0.011977467685937881,
-0.04478316009044647,
-0.08217740058898926,
-0.01617780141532421,
-0.05435249209403992,
0.05594515800476074,
0.04701656103134155,
-0.01850408874452114,
-0.028420444577932358,
0.010793156921863556,
-0.01428274717181921,
-0.03464016690850258,
0.057348910719156265,
-0.015439982526004314,
-0.04440338909626007,
0.018141621723771095,
0.43835175037384033,
-0.00043369887862354517,
-0.0044767167419195175,
-0.08949553221464157,
-0.005942900199443102,
0.018537545576691628,
-0.029883455485105515,
0.001426034839823842,
-0.02687288261950016,
-0.019304536283016205,
0.03952436149120331,
0.011246896348893642,
0.018041977658867836,
0.05000170320272446,
-0.022502917796373367,
-0.019157256931066513,
-0.04357415810227394,
0.014277910813689232,
-0.018248537555336952,
-0.006432818714529276,
0.03303205966949463,
0.0068274931982159615,
-0.0314582884311676,
0.00516762537881732,
-0.09094089269638062,
0.01959269307553768,
0.04576121270656586,
0.05486640706658363,
0.041820984333753586,
0.00797923281788826,
-0.022037770599126816,
0.05769150331616402,
-0.018858004361391068,
-0.007283445447683334,
-0.013775067403912544,
-0.004512816667556763,
0.02777242846786976,
0.02237304113805294,
-0.011745200492441654,
-0.006828964687883854,
-0.001819449127651751,
0.07627341151237488,
-0.09109307825565338,
-0.02450820989906788,
-0.06753455102443695,
-0.006768112536519766,
0.056138213723897934,
0.008213629014790058,
0.04085022211074829,
0.0006412660586647689,
0.02716296724975109,
-0.009304482489824295,
0.06979361176490784,
-0.04701573774218559,
-0.04286901280283928,
-0.0895187109708786,
0.023710452020168304,
-0.036297302693128586,
-0.006778830662369728,
0.04037350043654442,
-0.046690333634614944,
-0.05176945775747299,
-0.024217255413532257,
0.01776072569191456,
0.01779344491660595,
0.022412410005927086,
-0.016085967421531677,
0.01533630583435297,
0.004020515363663435,
0.046677716076374054,
0.005087129306048155,
-0.03823213651776314,
0.022675061598420143,
0.006114813033491373,
-0.07295215874910355,
-0.04719246178865433,
-0.06418845057487488,
-0.05540834367275238,
-0.019050998613238335,
0.017461074516177177,
0.0013001002371311188,
-0.011415760964155197,
0.014105956070125103,
-0.010983029380440712,
0.044627364724874496,
0.028284862637519836,
-0.023846272379159927,
-0.037411678582429886,
0.03542653098702431,
0.01199278049170971,
0.031933240592479706,
0.013044199906289577,
-0.018743444234132767,
-0.08529087901115417,
-0.014084403403103352,
0.01935970038175583,
-0.03429475799202919,
-0.010011364705860615,
-0.04716825857758522,
0.06609681993722916,
-0.04702534154057503,
0.06798373907804489,
0.014852995984256268,
0.018463049083948135,
0.0074900295585393906,
0.015001785941421986,
0.012641065753996372,
0.0683923289179802,
-0.025847258046269417,
0.07539444416761398,
-0.03679003193974495,
-0.051141660660505295,
0.003146893111988902,
-0.016974391415715218,
0.033262789249420166,
-0.004516143351793289,
0.0392114631831646,
-0.015702642500400543,
-0.016001729294657707,
0.08877568691968918,
0.027820253744721413,
0.015506035648286343,
0.014331151731312275,
0.01603834144771099,
-0.28531956672668457,
-0.06435445696115494,
-0.01933916099369526,
-0.0032847358379513025,
0.02684997022151947,
-0.04413492977619171,
-0.021952461451292038,
-0.03690534830093384,
0.003460579551756382,
0.03046857938170433,
0.06888559460639954,
0.06609990447759628,
0.009888719767332077,
-0.02079365961253643,
0.012386545538902283,
0.0560775026679039,
0.0807403102517128,
0.024196051061153412,
0.039508432149887085,
0.008596152998507023,
-0.00826347153633833,
-0.010651246644556522,
0.02657797560095787,
-0.0014849720755591989,
0.04419059678912163,
-0.002048490336164832,
0.15645720064640045,
0.05822553485631943,
-0.0521022230386734,
-0.04159826040267944,
0.0051667275838553905,
0.03806094080209732,
-0.02854708582162857,
-0.05921989679336548,
-0.009273474104702473,
-0.012649953365325928,
-0.028106609359383583,
-0.03544187173247337,
0.007383191492408514,
0.047626107931137085,
-0.010339953936636448,
-0.011613880284130573,
-0.015254526399075985,
-0.015261040069162846,
0.0051177009008824825,
0.00527866231277585,
0.012276798486709595,
0.14745289087295532,
-0.020393354818224907,
0.014807927422225475,
0.03598465397953987,
-0.017032116651535034,
0.00822536926716566,
-0.002617081394419074,
-0.027068840339779854,
-0.006210014224052429,
-0.06320853531360626,
-0.02311275526881218,
-0.049511685967445374,
-0.021037833765149117,
0.02027890272438526,
0.006668073125183582,
0.025943832471966743,
-0.03677767515182495,
-0.029170459136366844,
0.013725674711167812,
0.023808380588889122,
-0.01194552518427372,
0.01043462473899126,
0.027368349954485893,
-0.017245573922991753,
0.06231550872325897,
-0.012518498115241528,
0.08133533596992493,
0.036674682050943375,
0.06735759228467941,
-0.04380188509821892,
0.007258061785250902,
-0.04522352293133736,
0.0502786785364151,
0.006134301424026489,
-0.07447205483913422,
0.06858456879854202,
0.06572937965393066,
0.029179925099015236,
0.03732198849320412,
0.02096354402601719,
0.05468751862645149,
0.02910255454480648,
0.00998909305781126,
-0.0012397448299452662,
-0.014749232679605484,
-0.035711705684661865,
-0.017454540356993675,
-0.05714178830385208,
0.014650297351181507,
-0.30977341532707214,
0.022678349167108536,
0.03324926272034645,
-0.022563893347978592,
-0.036869700998067856,
-0.025629788637161255,
-0.013174391351640224,
-0.052200186997652054,
-0.01994387060403824,
0.005064701661467552,
0.04211695119738579,
0.0352567620575428,
0.03933199122548103,
0.044547248631715775,
0.007275847718119621,
0.016231488436460495,
0.020744429901242256,
0.013041561469435692,
-0.03776131942868233,
-0.02398419938981533,
0.010661163367331028,
-0.039302464574575424,
0.1641814261674881,
0.02049955539405346,
0.0060056354850530624,
0.022254379466176033,
-0.04633045941591263,
0.031548872590065,
-0.09201125055551529,
-0.03450417146086693,
-0.02414327673614025,
-0.024995220825076103,
0.00926386471837759,
-0.008709045127034187,
0.011179590597748756,
0.032072070986032486,
-0.03547480329871178,
0.05192605033516884,
-0.017434000968933105,
0.034852780401706696,
-0.053898878395557404,
0.0018466607434675097,
-0.007731501944363117,
-0.020102186128497124,
0.06513170897960663,
-0.055392321199178696,
-0.03917175531387329,
-0.03507056087255478,
-0.02913990430533886,
-0.002647985005751252,
-0.04363351687788963,
-0.014556589536368847,
0.03619750961661339,
-0.0639878660440445,
-0.009418955072760582,
-0.02782396785914898,
0.022623220458626747,
-0.023585546761751175,
0.021887430921196938,
0.011807173490524292,
0.06871887296438217,
0.027441849932074547,
-0.024417385458946228,
0.05049022659659386,
-0.010876866057515144
] |
PMID:8230 | Organic acidemias. | Inherited organic acidemias are a group of metabolic disorders currently being described and investigated as gas-liquid chromatography is applied to unexplained diseases of infancy and childhood. Common clinical presentations include attacks of ketoacidosis, unexplained metabolic acidosis, failure of normal development, seizures, and other neurologic abnormalities. Hyperglycinemia, hyperammonemia, and hypoglycemia are other laboratory findings frequently present. Diagnosis depends on examination of urine, and sometimes blood, by gas-liquid chromatography to measure concentrations of organic acids and organic acid derivatives. Prognosis in many cases is excellent if diagnosis is made promptly and the metabolic acidosis can be reversed. Recovery from neurologic deficits has frequently been seen. Long-term therapy is generally dependent on restricting precursors of the toxic organic acid which builds up as a consequence of the enzyme deficiency. If there is some enzyme activity retained or if alternate metabolic pathways exist, success with therapy is likely. | Organic acidemias. Inherited organic acidemias are a group of metabolic disorders currently being described and investigated as gas-liquid chromatography is applied to unexplained diseases of infancy and childhood. Common clinical presentations include attacks of ketoacidosis, unexplained metabolic acidosis, failure of normal development, seizures, and other neurologic abnormalities. Hyperglycinemia, hyperammonemia, and hypoglycemia are other laboratory findings frequently present. Diagnosis depends on examination of urine, and sometimes blood, by gas-liquid chromatography to measure concentrations of organic acids and organic acid derivatives. Prognosis in many cases is excellent if diagnosis is made promptly and the metabolic acidosis can be reversed. Recovery from neurologic deficits has frequently been seen. Long-term therapy is generally dependent on restricting precursors of the toxic organic acid which builds up as a consequence of the enzyme deficiency. If there is some enzyme activity retained or if alternate metabolic pathways exist, success with therapy is likely. | [
-0.008146869018673897,
0.025737592950463295,
0.00541294552385807,
0.06649093329906464,
-0.00151450140401721,
0.008974745869636536,
0.04568873718380928,
0.0332808718085289,
-0.06831134110689163,
-0.033582255244255066,
0.05211449787020683,
-0.053262531757354736,
0.006951252929866314,
0.0292937234044075,
-0.02273307926952839,
-0.0024421317502856255,
0.00861775130033493,
0.02267414703965187,
0.019661610946059227,
-0.03219550475478172,
-0.049458540976047516,
0.016094138845801353,
-0.020961539819836617,
-0.007323073223233223,
0.03508817031979561,
0.009089908562600613,
0.0030568945221602917,
-0.005498875863850117,
-0.08245284855365753,
-0.12219927459955215,
0.03805773705244064,
0.019527142867445946,
-0.003476385958492756,
-0.04840690642595291,
0.02204057201743126,
0.01182095892727375,
-0.018601369112730026,
0.036306384950876236,
-0.019167691469192505,
0.020305676385760307,
0.02539886347949505,
-0.02219434455037117,
0.03166719526052475,
-0.031842757016420364,
0.020040439441800117,
0.009442050941288471,
-0.02606986090540886,
0.022592490538954735,
0.11258308589458466,
-0.010000602342188358,
-0.06346093863248825,
0.005638957489281893,
0.04532267525792122,
0.03015623614192009,
-0.04160504415631294,
-0.013510375283658504,
0.06208516284823418,
-0.033915258944034576,
0.02281842939555645,
-0.0164495836943388,
0.018531912937760353,
0.026586005464196205,
-0.123997263610363,
0.02182723395526409,
0.04679042100906372,
0.058470264077186584,
-0.0008580092107877135,
-0.06909788399934769,
0.06113880127668381,
-0.027784941717982292,
-0.06450024247169495,
-0.00029483321122825146,
0.033825382590293884,
0.07943420857191086,
-0.017603090032935143,
-0.013387485407292843,
-0.005753257777541876,
-0.11391564458608627,
-0.02547621913254261,
-0.01685347408056259,
-0.029551677405834198,
-0.014914012514054775,
-0.0052778590470552444,
-0.0444365069270134,
0.0041309804655611515,
0.0015501028392463923,
-0.004461365286260843,
-0.01549449097365141,
0.05060591176152229,
0.023794909939169884,
-0.01364425290375948,
-0.05827083811163902,
0.0002783178351819515,
0.03447287529706955,
-0.06708498299121857,
0.005980429705232382,
0.025960782542824745,
-0.022201377898454666,
0.0024280245415866375,
0.4000988006591797,
-0.016146110370755196,
0.012581642717123032,
0.04790835455060005,
-0.025198550894856453,
-0.033090218901634216,
0.003733225865289569,
-0.002520470879971981,
-0.011540188454091549,
-0.007887390442192554,
-0.0396183617413044,
0.009293332695960999,
0.025139207020401955,
0.08448737859725952,
-0.06308676302433014,
-0.05189518257975578,
0.004063941538333893,
0.04825713112950325,
0.018337493762373924,
0.024656277149915695,
0.0788513571023941,
-0.02918051742017269,
-0.012120530009269714,
0.017188837751746178,
-0.00544159347191453,
0.0774296298623085,
0.05157601460814476,
-0.01609635166823864,
0.09014391899108887,
0.0008592333178967237,
0.04678354784846306,
0.0023634813260287046,
0.0072581279091537,
0.01654396764934063,
0.00888829492032528,
-0.0038780614268034697,
-0.021552035585045815,
0.06841222196817398,
-0.03208912909030914,
0.0438273660838604,
0.05885281041264534,
-0.04419182986021042,
0.03098120726644993,
-0.09105093032121658,
-0.02406335063278675,
-0.08550231903791428,
0.060565508902072906,
0.015152412466704845,
0.029237203299999237,
-0.017228523269295692,
0.0028391312807798386,
0.05237138643860817,
0.03729462996125221,
0.04107828065752983,
0.0676024928689003,
0.01101662777364254,
0.02792997844517231,
-0.031433671712875366,
-0.009042215533554554,
-0.022794388234615326,
0.03426552936434746,
-0.020884258672595024,
-0.03929014131426811,
-0.03583129495382309,
0.14032115042209625,
-0.004438319243490696,
0.021060984581708908,
-0.005071688909083605,
-0.059286292642354965,
-0.005382981151342392,
-0.01937193050980568,
0.005836318712681532,
-0.004366814158856869,
0.009904424659907818,
-0.03993901610374451,
-0.03133445605635643,
-0.03277326375246048,
-0.011084627360105515,
0.04445536062121391,
-0.037275128066539764,
-0.016640817746520042,
0.0703706294298172,
0.04529464244842529,
-0.05170290544629097,
-0.005355375818908215,
0.07071174681186676,
-0.027209250256419182,
-0.05995149910449982,
-0.011395626701414585,
0.055459070950746536,
0.05399659276008606,
-0.05875163897871971,
-0.012333198450505733,
-0.06468047201633453,
-0.059906598180532455,
0.06497135013341904,
0.015351209789514542,
-0.0013502435758709908,
-0.02046220190823078,
0.03923756629228592,
-0.016197344288229942,
0.09443295001983643,
0.06358406692743301,
0.05312382057309151,
0.05038510635495186,
0.018802374601364136,
0.03224688023328781,
0.011650670319795609,
-0.0242915116250515,
0.02191820554435253,
-0.014731106348335743,
-0.019067374989390373,
0.045016612857580185,
-0.058327317237854004,
0.03001442179083824,
-0.0853343978524208,
0.027639087289571762,
-0.04099061340093613,
0.0033808057196438313,
0.04551469162106514,
0.0029049424920231104,
0.07681208848953247,
-0.08384973555803299,
-0.014755470678210258,
-0.29510268568992615,
0.004644520580768585,
0.028618792071938515,
-0.022328775376081467,
0.004042887128889561,
0.016872582957148552,
0.014508893713355064,
-0.0072921402752399445,
-0.03798306733369827,
-0.018877938389778137,
0.017267871648073196,
0.0592547245323658,
-0.06900084763765335,
-0.06764460355043411,
-0.03032739646732807,
-0.018271012231707573,
0.015500664710998535,
-0.02716217003762722,
0.07451286166906357,
0.0250075850635767,
0.032475460320711136,
-0.019577085971832275,
0.044329747557640076,
-0.0035185350570827723,
0.0797278955578804,
-0.0001433024590369314,
0.08876634389162064,
-0.051389943808317184,
0.05639399215579033,
-0.06983143091201782,
0.02100486308336258,
0.04364680498838425,
-0.010876936838030815,
-0.07246057689189911,
-0.024674713611602783,
-0.07016861438751221,
-0.07208050042390823,
-0.09262808412313461,
-0.09418012946844101,
-0.05163537338376045,
-0.0985451191663742,
-0.07693133503198624,
-0.054848264902830124,
-0.02309957705438137,
-0.08700142055749893,
0.016933737322688103,
-0.01432472188025713,
0.00030806654831394553,
0.01082394365221262,
0.0050163911655545235,
0.03877953812479973,
-0.002973334165289998,
-0.03266562893986702,
-0.016305238008499146,
0.010688986629247665,
-0.01635177992284298,
-0.021760299801826477,
-0.022822346538305283,
-0.07936359941959381,
-0.06196758151054382,
-0.0000167374837474199,
0.04307018592953682,
0.0015819446416571736,
0.013258827850222588,
0.047330547124147415,
0.017641065642237663,
0.052854616194963455,
-0.010336529463529587,
0.06518050283193588,
0.027481259778141975,
-0.005560111720114946,
0.1283157616853714,
-0.0794934332370758,
-0.0008106275927275419,
0.023879041895270348,
0.03670619800686836,
-0.014259890653192997,
-0.02747480571269989,
-0.06076609715819359,
0.02551291696727276,
-0.028114942833781242,
-0.0685940608382225,
0.05139865726232529,
0.010927933268249035,
0.0022179819643497467,
0.06000683829188347,
0.006134155672043562,
0.008905836381018162,
-0.003243726212531328,
-0.028030190616846085,
0.018668390810489655,
0.025785140693187714,
-0.05648208409547806,
-0.029111018404364586,
0.007706806994974613,
0.04492761939764023,
-0.23737844824790955,
-0.0014404950197786093,
-0.08292553573846817,
0.005936849862337112,
0.04124799743294716,
0.06321587413549423,
0.006998302415013313,
0.03778264671564102,
-0.006993941031396389,
0.010028330609202385,
0.02526131458580494,
-0.03090842068195343,
0.06646766513586044,
-0.021090717986226082,
-0.0032967806328088045,
-0.0008135433308780193,
0.04437030106782913,
-0.044701267033815384,
0.013935000635683537,
-0.0012771583860740066,
0.0451652854681015,
-0.023080354556441307,
0.11840004473924637,
0.06695938110351562,
0.0003216183977201581,
0.02213498391211033,
-0.004866622854024172,
0.05232784152030945,
-0.040994856506586075,
-0.012250558473169804,
0.04157646372914314,
0.08168891072273254,
0.047064706683158875,
0.009512333199381828,
0.03622973710298538,
0.01316971704363823,
0.006829757709056139,
-0.05082622542977333,
0.01245487667620182,
0.008440791629254818,
-0.019650116562843323,
-0.02934878319501877,
-0.05749534070491791,
-0.054430071264505386,
0.025635598227381706,
0.0025861691683530807,
-0.05325654149055481,
-0.05860285088419914,
0.01607341319322586,
-0.006910028401762247,
0.005158844403922558,
-0.0802559033036232,
0.039010584354400635,
0.03401215001940727,
0.024622812867164612,
0.013135717250406742,
0.011783750727772713,
-0.0314529649913311,
0.0035044385585933924,
-0.03201998397707939,
0.03393229469656944,
-0.003811843693256378,
-0.01554839126765728,
0.08177784830331802,
-0.05687270313501358
] |
PMID:8231 | Antihypertensive action of propranolol in man: lack of evidence for a neural depressive effect. | The hypothesis that a neural depressive action is related to the antihypertensive effects of beta blockers has been evaluated in 14 essential hypertensive male patients through the circulatory response to noxious stimuli. The pressor reaction to mental arithmetic was primarily mediated by cardiac stimulation (beta receptors activation), that to cold by vasoconstriction (alpha receptors activation). Arithmetic and cold were tested to separate the effects of peripheral beta blackade from possible neural and other influences. After propanolol (320 mg per day for 3 wk): (1) The baseline pressure was reduced; (2) appearance, peak, and disappearance time of the circulatory reaction to either stimulus was not altered; (3) the pressor effect of arithmetic was decreased in an extent proportional to the reduced rise of cardiac output; and (4) pressure during cold reached the pretreatment levels through an augmented increase of vascular resistance. Our findings indicate that propranolol depresses only the circulatory reactions mediated through beta receptors activation and provide no evidence of effects other than beta blockade. | Antihypertensive action of propranolol in man: lack of evidence for a neural depressive effect. The hypothesis that a neural depressive action is related to the antihypertensive effects of beta blockers has been evaluated in 14 essential hypertensive male patients through the circulatory response to noxious stimuli. The pressor reaction to mental arithmetic was primarily mediated by cardiac stimulation (beta receptors activation), that to cold by vasoconstriction (alpha receptors activation). Arithmetic and cold were tested to separate the effects of peripheral beta blackade from possible neural and other influences. After propanolol (320 mg per day for 3 wk): (1) The baseline pressure was reduced; (2) appearance, peak, and disappearance time of the circulatory reaction to either stimulus was not altered; (3) the pressor effect of arithmetic was decreased in an extent proportional to the reduced rise of cardiac output; and (4) pressure during cold reached the pretreatment levels through an augmented increase of vascular resistance. Our findings indicate that propranolol depresses only the circulatory reactions mediated through beta receptors activation and provide no evidence of effects other than beta blockade. | [
-0.033534035086631775,
0.016144556924700737,
0.0019337532576173544,
0.02637568488717079,
-0.021252615377306938,
0.02789570577442646,
0.033062759786844254,
0.011445981450378895,
-0.0017339392798021436,
-0.10772506147623062,
-0.005558073054999113,
0.025182444602251053,
0.004668154753744602,
0.054641641676425934,
-0.06228560209274292,
-0.03313839063048363,
0.03653595596551895,
-0.010095825418829918,
-0.05831814184784889,
-0.02465030364692211,
-0.027987636625766754,
-0.0280078686773777,
0.02463107369840145,
-0.0056130955927073956,
-0.01976262964308262,
-0.0016110889846459031,
-0.0013465449446812272,
0.020570183172822,
-0.08836878836154938,
-0.17099560797214508,
0.03202551230788231,
0.002214089035987854,
0.078094482421875,
-0.0179066713899374,
-0.01529958937317133,
0.01694442704319954,
-0.03296458348631859,
0.05898328498005867,
-0.05055362731218338,
0.03886166214942932,
0.03183235973119736,
-0.035752616822719574,
-0.01780693791806698,
0.04353116452693939,
-0.00335091189481318,
-0.012935321778059006,
-0.029293477535247803,
-0.0028759303968399763,
0.09667186439037323,
-0.0066260057501494884,
0.021466655656695366,
-0.009751022793352604,
-0.017522303387522697,
0.06490189582109451,
0.017324546352028847,
-0.01614389941096306,
0.019597971811890602,
0.007677508518099785,
0.019145790487527847,
0.038406964391469955,
0.0025288083124905825,
0.03710605576634407,
-0.1826331913471222,
0.030759967863559723,
0.016717001795768738,
0.030792919918894768,
-0.037040892988443375,
0.00040834033279679716,
0.03798879310488701,
0.058965399861335754,
0.007969825528562069,
0.04051411151885986,
0.018359214067459106,
-0.01622146926820278,
-0.016158437356352806,
-0.01403097901493311,
-0.050636108964681625,
-0.006518854759633541,
0.006464396603405476,
-0.010251856409013271,
0.06703121960163116,
0.009458891116082668,
-0.00696425698697567,
-0.01984897442162037,
0.046661265194416046,
-0.07172247767448425,
0.029895640909671783,
-0.05706729739904404,
-0.028313608840107918,
-0.016671035438776016,
-0.07333872467279434,
-0.04054132103919983,
-0.02047007717192173,
0.002532425569370389,
0.0032224918249994516,
0.014167861081659794,
-0.03908322751522064,
-0.029787303879857063,
-0.057693205773830414,
0.3821895718574524,
0.031509626656770706,
0.03987066075205803,
-0.07180549204349518,
0.00882692914456129,
0.027287742123007774,
-0.024946408346295357,
0.006705404259264469,
-0.002866115653887391,
-0.027672329917550087,
0.047254983335733414,
0.0454883947968483,
-0.04749603196978569,
0.07871932536363602,
-0.02973400242626667,
0.006012676749378443,
-0.04380091652274132,
0.05920618399977684,
-0.05150216817855835,
0.016689326614141464,
-0.002814160892739892,
-0.0442507378757,
0.00760114099830389,
-0.02612091600894928,
0.02595384605228901,
0.02196936123073101,
0.000008505986443196889,
0.01868491992354393,
0.13040538132190704,
0.03330375999212265,
-0.021519768983125687,
0.08294260501861572,
0.0019607050344347954,
-0.036927565932273865,
0.030638571828603745,
0.02261338196694851,
-0.009107175283133984,
0.051953043788671494,
-0.026432815939188004,
0.0041612423956394196,
-0.015217073261737823,
0.030815117061138153,
0.0008674698183313012,
0.024634145200252533,
-0.04259223863482475,
-0.019919734448194504,
0.12197347730398178,
0.02633686736226082,
-0.03572063148021698,
-0.08174514025449753,
0.06263881921768188,
-0.07589750736951828,
0.008477195166051388,
-0.031215758994221687,
0.017995886504650116,
-0.022169068455696106,
-0.0038392762653529644,
0.010015469044446945,
0.006348098628222942,
-0.06368258595466614,
-0.013462194241583347,
-0.05677877366542816,
-0.037742625921964645,
-0.021783756092190742,
0.0037482487969100475,
-0.010353251360356808,
0.04355785250663757,
-0.022951828315854073,
-0.028347736224532127,
0.02337503433227539,
0.022816402837634087,
-0.0003574986185412854,
0.023754624649882317,
-0.0029935764614492655,
-0.05500185117125511,
-0.0011408500140532851,
0.032656099647283554,
0.07630583643913269,
-0.03290895000100136,
0.029369542375206947,
0.0012688706628978252,
0.01959518902003765,
-0.004784185905009508,
0.0470852367579937,
-0.023622162640094757,
-0.02450576238334179,
0.059306275099515915,
-0.0341048501431942,
-0.03877909854054451,
-0.01428203284740448,
0.04833726957440376,
-0.02642507664859295,
-0.031459491699934006,
-0.08496105670928955,
-0.02922554314136505,
-0.03727761283516884,
-0.0028891218826174736,
0.014514281414449215,
0.003341502044349909,
-0.0844016745686531,
0.016805438324809074,
0.02395462989807129,
-0.005940117407590151,
-0.014782488346099854,
0.01525653712451458,
0.054277729243040085,
0.05667421966791153,
-0.025089723989367485,
-0.0009482604800723493,
0.05404113605618477,
0.012154113501310349,
0.026360007002949715,
0.021718502044677734,
0.10256056487560272,
0.007774929516017437,
-0.04724288359284401,
0.015319433063268661,
0.002742303302511573,
0.009942103177309036,
0.04381279647350311,
0.04285367205739021,
0.02249273471534252,
-0.04389159381389618,
0.016313455998897552,
-0.3252338767051697,
-0.08191931992769241,
-0.005145180504769087,
-0.0271762702614069,
0.015062765218317509,
-0.0461554192006588,
0.04031503200531006,
-0.04892924800515175,
-0.029179876670241356,
-0.013206014409661293,
0.04725755751132965,
-0.002881064545363188,
-0.0033486871980130672,
0.01194312795996666,
0.011968319304287434,
0.055414922535419464,
0.028098568320274353,
-0.019557544961571693,
-0.016440127044916153,
0.02648758329451084,
-0.038535259664058685,
0.004286313895136118,
0.006155880633741617,
0.016187014058232307,
0.014529531821608543,
-0.003014894435182214,
0.06509547680616379,
0.017752911895513535,
-0.004361658822745085,
0.014362354762852192,
-0.013833172619342804,
0.06391264498233795,
0.01789047010242939,
-0.13368719816207886,
0.04336140304803848,
-0.04946703091263771,
0.055951718240976334,
0.00471292482689023,
0.009750944562256336,
0.041885294020175934,
-0.045264169573783875,
0.02339751087129116,
0.01468795444816351,
0.03628063201904297,
-0.06808705627918243,
0.019896473735570908,
0.0667065978050232,
0.04949362203478813,
-0.07957924157381058,
0.02903737872838974,
0.0029508545994758606,
0.005226743873208761,
0.016995489597320557,
0.0002683008788153529,
0.05386115983128548,
-0.022688983008265495,
-0.0950879380106926,
-0.01186236459761858,
0.010942530818283558,
0.003643226344138384,
0.0318232923746109,
0.014843614771962166,
0.05579470098018646,
-0.011535659432411194,
-0.04586002603173256,
0.0564412921667099,
0.04427305981516838,
0.021837284788489342,
0.054048314690589905,
0.02305753156542778,
0.03201925382018089,
0.10967724770307541,
-0.0089913634583354,
0.017545858398079872,
0.10261663794517517,
-0.0015338020166382194,
-0.006797941867262125,
-0.03430498018860817,
-0.03475057706236839,
0.010882141999900341,
-0.0021786498837172985,
-0.06746526062488556,
0.010829667560756207,
0.04457578435540199,
-0.016079582273960114,
-0.045755013823509216,
0.025316592305898666,
-0.007984180003404617,
0.07713937759399414,
-0.03792658448219299,
-0.032130442559719086,
0.02093529887497425,
-0.04855285957455635,
-0.06299615651369095,
0.07541406154632568,
0.030204812064766884,
-0.2629145085811615,
-0.03828379884362221,
0.05986364185810089,
-0.029199015349149704,
0.012710743583738804,
0.021172653883695602,
0.025809748098254204,
-0.03621980547904968,
-0.06603559106588364,
-0.012745434418320656,
-0.057331740856170654,
0.047099191695451736,
0.09162264317274094,
0.06510183215141296,
-0.09836293756961823,
-0.015872851014137268,
0.016900312155485153,
-0.05180501937866211,
0.03400754928588867,
-0.05614304915070534,
-0.014901135116815567,
0.02360636182129383,
0.14389075338840485,
-0.003535035066306591,
-0.029914002865552902,
0.05667106434702873,
0.03545496240258217,
0.005395142827183008,
-0.023987865075469017,
0.009332363493740559,
-0.04937349632382393,
0.030853860080242157,
0.044027380645275116,
-0.051862116903066635,
-0.001368158613331616,
0.024737078696489334,
-0.061743929982185364,
-0.025442611426115036,
0.02950425073504448,
0.009665066376328468,
0.009158709086477757,
0.008380971848964691,
-0.04457110911607742,
-0.0034629995934665203,
0.059533875435590744,
-0.05448514223098755,
-0.013607945293188095,
-0.017041310667991638,
-0.030504053458571434,
0.024963688105344772,
-0.023345358669757843,
-0.0076801530085504055,
0.05584684759378433,
0.02086755447089672,
-0.03656265139579773,
-0.0224933922290802,
-0.046517111361026764,
-0.03568487986922264,
0.02159862592816353,
-0.04186330735683441,
-0.02134832926094532,
-0.034067898988723755,
-0.020939763635396957,
0.057207297533750534,
0.06529252231121063
] |
PMID:8229 | Calcificaiton of growth plate cartilage with special reference to studies on micropuncture fluids. | No final comprehensive hypothesis of the chain of events involved in inhibition of calcification can be constructed at the present time. The flow diagram in Figure 1 is presented only as a working hypothesis currently used by the authors. Insofar as the Cfl is representative of native factors at calcifying sites, none of the major theories on the nature of mineral formation at present seem excluded. As in the fields of clotting and complement pathways, where backup systems seem to operate, one should not be surprised if more than one system operates in cartilaginous calcification. | Calcificaiton of growth plate cartilage with special reference to studies on micropuncture fluids. No final comprehensive hypothesis of the chain of events involved in inhibition of calcification can be constructed at the present time. The flow diagram in Figure 1 is presented only as a working hypothesis currently used by the authors. Insofar as the Cfl is representative of native factors at calcifying sites, none of the major theories on the nature of mineral formation at present seem excluded. As in the fields of clotting and complement pathways, where backup systems seem to operate, one should not be surprised if more than one system operates in cartilaginous calcification. | [
-0.040445782244205475,
-0.02548879384994507,
0.00245736469514668,
0.03625382110476494,
-0.04395776614546776,
-0.023981193080544472,
0.032435521483421326,
0.06553088128566742,
-0.03197602555155754,
-0.0030801682732999325,
0.0017347236862406135,
-0.11215126514434814,
0.0008834772743284702,
0.04327797889709473,
0.010400745086371899,
-0.0017047678120434284,
-0.012163407169282436,
0.03990059718489647,
-0.04524275287985802,
0.009315156377851963,
0.02931714989244938,
-0.024564700201153755,
-0.011344711296260357,
-0.031502220779657364,
0.017089713364839554,
0.02920413389801979,
-0.022362418472766876,
-0.08250141143798828,
0.018943898379802704,
-0.19784830510616302,
-0.024508915841579437,
-0.056040506809949875,
0.05655444785952568,
-0.06218530982732773,
0.0031352920923382044,
0.017699938267469406,
-0.03198402002453804,
0.035043612122535706,
-0.03136550635099411,
0.019085338339209557,
0.022317558526992798,
0.05655897408723831,
-0.01556933019310236,
0.06682969629764557,
0.052124496549367905,
-0.009432059712707996,
0.0015547553775832057,
0.011204374954104424,
0.03233027085661888,
0.04586784169077873,
0.016721202060580254,
-0.015946829691529274,
-0.0077216546051204205,
0.06022816523909569,
-0.01613135077059269,
0.01494776364415884,
0.04292863607406616,
0.0055399201810359955,
0.09096744656562805,
0.00698664179071784,
0.04317079856991768,
0.07404106110334396,
-0.13105815649032593,
0.06881478428840637,
0.04623354598879814,
0.07864204794168472,
0.03468748554587364,
-0.034914951771497726,
0.039081986993551254,
0.04877043515443802,
-0.006804687902331352,
0.04880380257964134,
-0.03420931100845337,
0.01460307464003563,
0.057025596499443054,
-0.054131343960762024,
0.017720652744174004,
-0.018778681755065918,
-0.04121292009949684,
0.05565841495990753,
-0.0073589151725173,
0.03568686544895172,
0.01107281818985939,
0.0320538692176342,
-0.0068313442170619965,
-0.03080783225595951,
0.011023600585758686,
-0.0394483283162117,
-0.07185810804367065,
0.017154676839709282,
-0.016052480787038803,
-0.04759129881858826,
0.004636276513338089,
-0.007086905650794506,
-0.09000730514526367,
-0.002835851861163974,
0.04869254678487778,
0.02536807209253311,
0.03918025642633438,
0.37048619985580444,
-0.006986791733652353,
0.00489969365298748,
-0.00865029077976942,
0.08898566663265228,
0.05598312243819237,
-0.008812610991299152,
-0.04607776552438736,
-0.035586610436439514,
0.009567742235958576,
0.04535704106092453,
0.055323291569948196,
0.026703422889113426,
-0.022395852953195572,
-0.04065863788127899,
-0.014797999523580074,
-0.02822187915444374,
0.036473605781793594,
0.0127719109877944,
-0.04088389128446579,
0.050104375928640366,
-0.023661648854613304,
0.005871123168617487,
-0.006781105417758226,
-0.028356540948152542,
-0.006280010100454092,
0.022570215165615082,
-0.017780790105462074,
0.05161646381020546,
0.041221003979444504,
-0.03676551207900047,
0.040879733860492706,
-0.02677510492503643,
-0.004125472158193588,
0.03715106472373009,
0.0013611719477921724,
-0.004385640844702721,
0.03151707351207733,
-0.026395512744784355,
-0.013442201539874077,
-0.026714105159044266,
-0.014555521309375763,
-0.019800199195742607,
-0.02720801904797554,
-0.060079194605350494,
-0.00908814836293459,
0.08056515455245972,
-0.024005742743611336,
0.040782175958156586,
-0.010722344741225243,
0.010702411644160748,
-0.05162322521209717,
0.0010987251298502088,
-0.02499738149344921,
-0.0005445434362627566,
-0.05664801597595215,
-0.00716074975207448,
-0.022295121103525162,
-0.0042549194768071175,
-0.017390282824635506,
-0.0125576825812459,
-0.03409986570477486,
-0.001027173944748938,
-0.016236895695328712,
0.10292836278676987,
0.02226872369647026,
-0.035810355097055435,
0.013324708677828312,
0.04148232936859131,
0.009396791458129883,
-0.11087716370820999,
-0.040022458881139755,
0.017215169966220856,
-0.023830600082874298,
-0.08689095079898834,
0.031696867197752,
0.05260061100125313,
-0.04778064414858818,
0.03470708057284355,
0.02848251350224018,
0.04130490496754646,
0.057357996702194214,
0.000875446479767561,
-0.012693139724433422,
0.046428319066762924,
0.023801209405064583,
-0.1165328249335289,
-0.036890916526317596,
0.015340900048613548,
0.010337200947105885,
0.05505024641752243,
-0.01972259022295475,
-0.05943211540579796,
-0.07058394700288773,
0.053765371441841125,
-0.024716706946492195,
-0.02990785241127014,
0.014884975738823414,
-0.05019635707139969,
-0.07431476563215256,
-0.016976861283183098,
0.030263589695096016,
0.01520484872162342,
-0.015937309712171555,
0.03691238909959793,
-0.014500888995826244,
0.019326716661453247,
0.02514028176665306,
0.028220538049936295,
-0.010345200076699257,
-0.0031867213547229767,
-0.046273503452539444,
0.019543400034308434,
-0.08193367719650269,
-0.06986760348081589,
0.0055040037259459496,
0.046888209879398346,
-0.01311253197491169,
-0.026313193142414093,
0.010630470700562,
-0.05884933844208717,
0.07258003950119019,
0.003073766129091382,
-0.05334944278001785,
-0.34406208992004395,
0.0005080195842310786,
0.055404145270586014,
0.015265002846717834,
0.09090927243232727,
-0.0784367024898529,
0.030866287648677826,
-0.002767863217741251,
-0.05667679384350777,
-0.023178083822131157,
0.0045440648682415485,
0.11414450407028198,
-0.020682958886027336,
-0.01571895368397236,
0.012829716317355633,
-0.01980738714337349,
0.04349943622946739,
-0.06276760995388031,
0.0009861598955467343,
-0.005135557148605585,
0.030767111107707024,
0.009323464706540108,
-0.007530077360570431,
0.019886799156665802,
0.023435162380337715,
0.02411283738911152,
0.11251688003540039,
0.0024482225999236107,
-0.021955661475658417,
-0.04877450689673424,
0.01676270365715027,
0.03285439312458038,
-0.03457225114107132,
-0.05731191113591194,
-0.015144014731049538,
-0.003418689826503396,
0.020916175097227097,
-0.03814536705613136,
0.013457230292260647,
0.007166164927184582,
-0.0339261032640934,
0.004962397739291191,
-0.07606486231088638,
-0.007850565947592258,
-0.014799998141825199,
-0.0025034802965819836,
-0.030532941222190857,
0.004837786313146353,
0.03291189670562744,
-0.005696485284715891,
0.04049051180481911,
-0.008654603734612465,
0.018980294466018677,
0.0007781824097037315,
0.006079634185880423,
0.018794158473610878,
-0.018071962520480156,
0.03687717765569687,
-0.044580187648534775,
-0.06768960505723953,
-0.03240717947483063,
0.0035112136974930763,
0.05489886924624443,
0.020095136016607285,
0.03568060323596001,
-0.04678166285157204,
-0.024979354813694954,
-0.01953718811273575,
0.05521729215979576,
0.0011489721946418285,
0.006376916542649269,
0.10797516256570816,
-0.0018941541202366352,
0.047326382249593735,
0.029792141169309616,
0.005074867978692055,
0.0102933244779706,
-0.01712513528764248,
-0.024014730006456375,
-0.0065611582249403,
0.045819565653800964,
-0.09149650484323502,
0.07319870591163635,
0.03073124587535858,
-0.014118550345301628,
0.011618716642260551,
0.015550323761999607,
-0.04672082141041756,
0.021299488842487335,
0.011403442360460758,
0.019948920235037804,
-0.029841134324669838,
-0.03966616839170456,
-0.054349131882190704,
0.09300601482391357,
0.02888878993690014,
-0.2542071044445038,
0.01622879132628441,
0.07732761651277542,
-0.02064366266131401,
0.012445911765098572,
-0.01419452391564846,
0.032415274530649185,
-0.004294826649129391,
0.07972108572721481,
0.054316192865371704,
-0.006906190887093544,
0.013178694993257523,
0.04091893881559372,
0.028595803305506706,
-0.029576536267995834,
0.0392087884247303,
0.09866701066493988,
-0.03773410618305206,
0.010694990865886211,
-0.03487592563033104,
-0.030456822365522385,
-0.06174454465508461,
0.1398579627275467,
0.020782863721251488,
0.01643708348274231,
0.027847277000546455,
0.023357434198260307,
-0.03973039984703064,
-0.04530392587184906,
-0.033169686794281006,
-0.0028613500762730837,
0.004130831453949213,
-0.008399824611842632,
0.01534485723823309,
-0.00732829375192523,
0.049803946167230606,
0.022922689095139503,
0.058713387697935104,
0.008803476579487324,
0.017145296558737755,
-0.009530328214168549,
-0.007751006633043289,
-0.08907154202461243,
-0.07835163921117783,
0.05207342654466629,
-0.10154073685407639,
0.01737982966005802,
-0.03503233939409256,
0.04007711261510849,
0.0019160869996994734,
-0.028499111533164978,
-0.02579936385154724,
0.04455767944455147,
-0.011212018318474293,
-0.07114899903535843,
0.019494660198688507,
-0.0058419592678546906,
-0.029174435883760452,
0.025844629853963852,
-0.005071192514151335,
0.014648891985416412,
-0.05019281059503555,
-0.027623774483799934,
0.07202521711587906,
-0.018025681376457214
] |
PMID:8232 | Clinical pharmacokinetics of lorazepam. I. Absorption and disposition of oral 14C-lorazepam. | Eight healthy male subjects received single 2-mg oral doses of lorazepam containing 24 muCi/mg of 2-14C-lorazepam. Multiple venous blood samples were drawn during the first 96 hr after the dose, and all urine and stool were collected for 120 hr after dosing. Concentrations of lorazepam and its metabolites in body fluids were determined by appropriate analytic techniques. Following a lag time, lorazepam was absorbed with an apparent first-order half-life of 15 min. The peak plasma concentration was 16.9 ng/ml, measured in the pooled sample drawn 2 hr after the dose, This corresponded to the time at which clinical effects appeared to be maximal. The apparent elimination half-life of lorazepam was about 12 hr. Biotransformation to a pharmacologically inactive glucuronide metabolite appeared to be the major mechanism of lorazepam clearance. A mean of 88% of administered radioactivity was recovered in urine, and 7% was recovered in stool. Lorazepam glucuronide comprised 86% of urinary reactivity; its renal clearance was 37 ml/min. Other identified metabolites included hydroxylorazepam, a quinazolinone derivative, and a quinazoline carboxylic acid; all of these were quantitatively minor. | Clinical pharmacokinetics of lorazepam. I. Absorption and disposition of oral 14C-lorazepam. Eight healthy male subjects received single 2-mg oral doses of lorazepam containing 24 muCi/mg of 2-14C-lorazepam. Multiple venous blood samples were drawn during the first 96 hr after the dose, and all urine and stool were collected for 120 hr after dosing. Concentrations of lorazepam and its metabolites in body fluids were determined by appropriate analytic techniques. Following a lag time, lorazepam was absorbed with an apparent first-order half-life of 15 min. The peak plasma concentration was 16.9 ng/ml, measured in the pooled sample drawn 2 hr after the dose, This corresponded to the time at which clinical effects appeared to be maximal. The apparent elimination half-life of lorazepam was about 12 hr. Biotransformation to a pharmacologically inactive glucuronide metabolite appeared to be the major mechanism of lorazepam clearance. A mean of 88% of administered radioactivity was recovered in urine, and 7% was recovered in stool. Lorazepam glucuronide comprised 86% of urinary reactivity; its renal clearance was 37 ml/min. Other identified metabolites included hydroxylorazepam, a quinazolinone derivative, and a quinazoline carboxylic acid; all of these were quantitatively minor. | [
0.0015740356175228953,
0.012197461910545826,
0.023623168468475342,
0.010751262307167053,
0.018865494057536125,
-0.03865942358970642,
0.055533863604068756,
0.020784329622983932,
-0.008962766267359257,
-0.038765259087085724,
0.014137041755020618,
-0.041129015386104584,
0.032701462507247925,
0.041228510439395905,
-0.003885551355779171,
-0.0029833142179995775,
0.07003168016672134,
-0.036472387611866,
-0.004595619160681963,
-0.00975391548126936,
0.003188175382092595,
-0.02974734641611576,
0.03552594408392906,
0.031381089240312576,
0.06948067247867584,
0.04076492413878441,
0.015975380316376686,
0.060640767216682434,
-0.06891573965549469,
-0.16664037108421326,
0.034409936517477036,
0.055809032171964645,
-0.008847593329846859,
-0.0055968500673770905,
-0.07163248211145401,
0.020776575431227684,
-0.020514609292149544,
0.052848558872938156,
0.02563641220331192,
0.057853471487760544,
0.07900463789701462,
0.04091622680425644,
-0.013986564241349697,
0.010598096996545792,
-0.001228068140335381,
-0.022306008264422417,
-0.10948728024959564,
0.04196681082248688,
0.14537760615348816,
0.03893505409359932,
-0.03673965483903885,
0.037053633481264114,
0.024251572787761688,
0.05840053781867027,
-0.02565605752170086,
-0.0380919948220253,
0.02518646791577339,
-0.0720687136054039,
-0.04647056758403778,
0.05699131265282631,
-0.037273190915584564,
0.01824638992547989,
-0.1327989548444748,
0.05134332925081253,
-0.012004337273538113,
-0.03688056394457817,
-0.007623431272804737,
-0.042573921382427216,
0.05214468389749527,
0.10811294615268707,
-0.07311218976974487,
0.0383935384452343,
-0.04219168424606323,
0.007534515578299761,
0.05548728629946709,
0.0014230167726054788,
0.07100529968738556,
0.009754465892910957,
0.012984637171030045,
-0.03416099399328232,
0.01955951191484928,
0.021267544478178024,
0.04444858431816101,
-0.052856165915727615,
0.027610469609498978,
-0.0052649457938969135,
0.022311165928840637,
-0.005490564741194248,
0.004406667314469814,
0.028063939884305,
0.06334350258111954,
-0.04971317574381828,
-0.03422064706683159,
-0.045942142605781555,
-0.015503237955272198,
-0.017731020227074623,
-0.007278337609022856,
-0.02104700542986393,
-0.007479181047528982,
0.34109896421432495,
-0.0027945376932621002,
-0.000003985617240687134,
-0.02997301146388054,
-0.003863649209961295,
0.01700475998222828,
0.026612384244799614,
0.01695840060710907,
-0.019697386771440506,
0.02398073859512806,
0.018939102068543434,
0.015088886022567749,
-0.010061410255730152,
0.09138555079698563,
-0.018607648089528084,
-0.015419714152812958,
0.049140654504299164,
0.03290446475148201,
-0.01298225112259388,
0.006627713795751333,
0.001105707255192101,
-0.043820325285196304,
-0.00958667416125536,
-0.009535186924040318,
-0.05314421281218529,
0.048849646002054214,
0.003495500423014164,
-0.004327444825321436,
0.1299692988395691,
0.029154915362596512,
0.04994521290063858,
0.0007209418690763414,
-0.03891999274492264,
0.025263691321015358,
0.025718269869685173,
0.011514963582158089,
-0.04011617600917816,
0.07177433371543884,
-0.008820904418826103,
-0.0014124993467703462,
0.01146655436605215,
0.027915436774492264,
-0.03724685311317444,
-0.019068291410803795,
-0.09483467787504196,
-0.06720130890607834,
0.16949984431266785,
-0.005266789346933365,
-0.048537760972976685,
-0.015189047902822495,
-0.02757834643125534,
-0.0029840264469385147,
0.040066011250019073,
0.0533563457429409,
0.0029967138543725014,
-0.04473371431231499,
0.03379278630018234,
0.01880384422838688,
0.03614770993590355,
-0.02224004827439785,
0.03288440406322479,
-0.051631007343530655,
-0.028298137709498405,
-0.028510024771094322,
0.08549409359693527,
-0.004243650007992983,
0.01967303268611431,
-0.010724266059696674,
0.005147737916558981,
0.007865906693041325,
0.049857355654239655,
0.035531651228666306,
-0.031557731330394745,
-0.01435183547437191,
-0.02006525732576847,
-0.029059460386633873,
-0.0007207603775896132,
0.029879871755838394,
-0.01329790335148573,
-0.01323307678103447,
-0.009320023469626904,
0.015872560441493988,
0.06299892067909241,
-0.030175523832440376,
0.004260169807821512,
0.05226319283246994,
-0.021557224914431572,
-0.002524169161915779,
0.005575125105679035,
-0.014315038919448853,
0.045067861676216125,
-0.04251346364617348,
-0.03272075578570366,
-0.08260549604892731,
-0.014600932598114014,
-0.07198215276002884,
-0.05391380563378334,
-0.10759637504816055,
0.028360214084386826,
-0.03143400698900223,
-0.05691870301961899,
0.09485140442848206,
0.07714039832353592,
-0.012428178451955318,
0.06425327807664871,
0.0031205820851027966,
0.06870397925376892,
-0.026812521740794182,
0.03550145775079727,
0.050472553819417953,
0.003981532994657755,
0.057073019444942474,
-0.012016333639621735,
-0.005284943152219057,
0.03936060145497322,
0.013384130783379078,
0.056286804378032684,
0.019528424367308617,
-0.04830930009484291,
0.036690354347229004,
-0.008924085646867752,
0.01312986295670271,
-0.06749554723501205,
0.0094198202714324,
-0.295747309923172,
-0.028608005493879318,
0.05010233446955681,
0.029459118843078613,
0.06140674650669098,
-0.060621198266744614,
-0.017259841784834862,
-0.031187912449240685,
-0.006237058434635401,
-0.02913277968764305,
0.0038648478221148252,
0.03694644942879677,
-0.08751977235078812,
-0.05271953344345093,
-0.03863690420985222,
-0.00001361115209874697,
0.04851025342941284,
0.01903819479048252,
0.029464127495884895,
-0.0742536261677742,
0.007140832953155041,
-0.02356451004743576,
-0.05660860240459442,
0.023581037297844887,
0.025125274434685707,
-0.012805654667317867,
0.10985850542783737,
-0.01903224177658558,
-0.07331513613462448,
-0.036447808146476746,
0.0685039535164833,
0.05907360091805458,
0.0029854625463485718,
-0.10365872085094452,
-0.015334632247686386,
-0.0239401962608099,
-0.029247518628835678,
-0.034247059375047684,
0.02063599042594433,
0.005746044684201479,
-0.08548478037118912,
0.022596202790737152,
0.04580272361636162,
-0.0912092924118042,
-0.06523669511079788,
0.009106775745749474,
0.007940109819173813,
-0.025427600368857384,
-0.001245258841663599,
-0.04208654910326004,
0.01409443374723196,
-0.0017931811744347215,
0.021419862285256386,
-0.03641042113304138,
0.024100136011838913,
-0.003841809695586562,
-0.04374651238322258,
0.012292384169995785,
-0.06203213334083557,
-0.02315405011177063,
-0.06344907730817795,
0.021074118092656136,
0.03948834538459778,
0.012271115556359291,
0.02024594508111477,
-0.024225959554314613,
0.002292569261044264,
0.03701149299740791,
0.037071533501148224,
-0.005045206286013126,
-0.0014201459707692266,
0.10574743151664734,
-0.06866472214460373,
-0.0006832042126916349,
0.03589004650712013,
0.009510205127298832,
-0.021756598725914955,
-0.01972135528922081,
-0.09303232282400131,
-0.014264076948165894,
0.004554744344204664,
0.02786237560212612,
0.038285668939352036,
0.02320793643593788,
0.06125747039914131,
-0.008967360481619835,
0.025879541411995888,
-0.060801394283771515,
-0.03333735838532448,
0.0018622149946168065,
0.02962823212146759,
0.008092292584478855,
-0.04047229140996933,
-0.07140558212995529,
-0.004696336574852467,
0.06485655158758163,
-0.22794845700263977,
-0.016263827681541443,
-0.0033080773428082466,
0.1082673892378807,
0.032963450998067856,
-0.051448073238134384,
0.07665430009365082,
0.020816536620259285,
-0.04628150165081024,
-0.01474019419401884,
0.015338982455432415,
0.03598463535308838,
0.06030299887061119,
-0.002398258075118065,
-0.004647452849894762,
0.025921160355210304,
0.07082188129425049,
-0.017684193328022957,
-0.06952571868896484,
0.015158334746956825,
0.0048267231322824955,
-0.01096623670309782,
0.04706726595759392,
0.0034656748175621033,
0.014666171744465828,
0.04331359267234802,
-0.04666193574666977,
0.012025428004562855,
-0.01159489993005991,
0.02300582267343998,
-0.06453541666269302,
0.04269023239612579,
0.10102210193872452,
-0.08147308230400085,
-0.004974306095391512,
0.005556826014071703,
-0.05156223475933075,
-0.014832642860710621,
-0.0015463674208149314,
0.00815572403371334,
-0.008176552131772041,
-0.0004883736255578697,
-0.011850648559629917,
-0.005000070668756962,
0.055269572883844376,
-0.1164059117436409,
-0.025831976905465126,
-0.0950586125254631,
-0.006781790871173143,
-0.00515403039753437,
-0.04112423583865166,
-0.012217959389090538,
-0.033308159559965134,
0.021246062591671944,
0.06456918269395828,
0.022288672626018524,
-0.010827897116541862,
-0.06973131746053696,
0.029719552025198936,
-0.021225105971097946,
-0.043080784380435944,
-0.009586079977452755,
0.01279644574970007,
0.02043219655752182,
-0.03689270839095116
] |
PMID:8233 | Effect of age on oxygen-binding in normal human subjects. | 1. Oxygen-binding, plasma and intra-erythrocytic pH, and haemoglobin, 2,3-diphosphoglycerate and inorganic phosphate concentrations were measured in sixty-two healthy non-smokers aged between 18 and 89 years. 2. P50 (oxygen tension at 50% oxygen saturation) expressed at plasma pH 7-40 and PCO2 5-33 kPa showed a positive correlation with age. 3. This correlation of P50 with age was closer when P50 was expressed at a constant intra-erythrocytic pH 7-20. On average P50 at intra-erythrocytic pH 7-20 increased from 3-59 kPa at 20 years to 3-96 kPa at 90 years of age. 4. 2,3-Diphosphoglycerate, inorganic phosphate, haemoglobin and mean corpuscular haemoglobin concentrations did not correlate with P50 or with age. | Effect of age on oxygen-binding in normal human subjects. 1. Oxygen-binding, plasma and intra-erythrocytic pH, and haemoglobin, 2,3-diphosphoglycerate and inorganic phosphate concentrations were measured in sixty-two healthy non-smokers aged between 18 and 89 years. 2. P50 (oxygen tension at 50% oxygen saturation) expressed at plasma pH 7-40 and PCO2 5-33 kPa showed a positive correlation with age. 3. This correlation of P50 with age was closer when P50 was expressed at a constant intra-erythrocytic pH 7-20. On average P50 at intra-erythrocytic pH 7-20 increased from 3-59 kPa at 20 years to 3-96 kPa at 90 years of age. 4. 2,3-Diphosphoglycerate, inorganic phosphate, haemoglobin and mean corpuscular haemoglobin concentrations did not correlate with P50 or with age. | [
-0.06606733053922653,
0.05779964476823807,
0.060673993080854416,
0.014032999984920025,
0.001824889681302011,
-0.03041258081793785,
0.08885151147842407,
-0.0014499605167657137,
-0.074225515127182,
0.022049997001886368,
0.04742342233657837,
-0.07900399714708328,
-0.01802211068570614,
0.040521834045648575,
-0.06544715911149979,
0.015193911269307137,
-0.010191375389695168,
-0.03905579075217247,
-0.09762965887784958,
0.06477168947458267,
-0.012487517669796944,
0.030489612370729446,
0.005491625517606735,
-0.01421584002673626,
0.06315548717975616,
0.058180294930934906,
-0.037963658571243286,
-0.008659166283905506,
-0.06775294989347458,
-0.15121686458587646,
0.002677979413419962,
0.021696779876947403,
0.025609930977225304,
-0.006685746368020773,
-0.059611428529024124,
0.05815500766038895,
-0.07443753629922867,
0.013974134810268879,
-0.030495475977659225,
0.058392636477947235,
0.045104607939720154,
0.03439950570464134,
-0.03484375402331352,
0.04643440619111061,
-0.03054216131567955,
0.015440373681485653,
-0.07140345871448517,
0.008650926873087883,
0.06631721556186676,
0.06668324023485184,
-0.026269588619470596,
0.054010841995477676,
-0.02530129812657833,
0.02544182911515236,
-0.031769197434186935,
-0.03639978542923927,
0.011785509064793587,
0.01646454446017742,
0.044588908553123474,
0.0031196116469800472,
-0.03093084879219532,
0.015475768595933914,
-0.15566612780094147,
0.09112018346786499,
0.11331821233034134,
-0.0023587453179061413,
0.028605135157704353,
-0.0008520159171894193,
0.02659909427165985,
-0.02206532470881939,
-0.06124040484428406,
0.07999685406684875,
0.0085340216755867,
-0.016902588307857513,
-0.004486862104386091,
-0.006908268202096224,
-0.02587239257991314,
-0.0575430728495121,
0.044117528945207596,
-0.0097332913428545,
-0.003064766526222229,
0.06019558385014534,
-0.017958689481019974,
-0.007015975657850504,
-0.015874354168772697,
0.008769206702709198,
-0.008904991671442986,
-0.0184843260794878,
-0.05452350899577141,
-0.021950362250208855,
-0.027967117726802826,
-0.04753553867340088,
-0.055438652634620667,
0.05450902134180069,
-0.022313358262181282,
0.0665339007973671,
0.05368863418698311,
0.00010902965732384473,
-0.051539383828639984,
0.3633640706539154,
-0.0574265792965889,
0.039832521229982376,
-0.00518643157556653,
0.029513945803046227,
0.02352026291191578,
-0.06792765110731125,
0.0260652843862772,
0.04289187863469124,
-0.025851640850305557,
0.05832086503505707,
-0.031175430864095688,
-0.016660811379551888,
0.026732150465250015,
-0.061220187693834305,
-0.026117265224456787,
0.0551108792424202,
0.02342979609966278,
0.06052554026246071,
0.007720372639596462,
0.021280363202095032,
-0.004119138699024916,
-0.04334810748696327,
-0.020784201100468636,
0.016424639150500298,
0.09882272779941559,
0.021342365071177483,
0.011690011247992516,
0.10359220206737518,
0.03782717511057854,
-0.011262282729148865,
0.02866286225616932,
-0.006130747497081757,
-0.0560687817633152,
0.0031556272879242897,
-0.02083008922636509,
-0.01781199313700199,
0.01940269023180008,
0.0007027774699963629,
0.023936359211802483,
0.0015289554139599204,
-0.001160882879048586,
-0.040081147104501724,
-0.03351028636097908,
-0.03607381880283356,
-0.04186931625008583,
0.11046461015939713,
0.01292096171528101,
0.03115573711693287,
-0.005824672989547253,
-0.052436232566833496,
0.008986886590719223,
0.07750260084867477,
0.003648586804047227,
0.06264042109251022,
-0.028066273778676987,
0.007341815158724785,
0.012171159498393536,
-0.011991836130619049,
0.022125137969851494,
-0.0784846618771553,
-0.05757474899291992,
-0.025847189128398895,
-0.05095614492893219,
-0.01330128125846386,
0.007794716861099005,
-0.007179108913987875,
-0.013009653426706791,
-0.0016880438197404146,
0.04033832252025604,
0.02416854165494442,
0.0634412169456482,
-0.038410484790802,
0.05807945132255554,
-0.017713336274027824,
0.06652029603719711,
0.039233364164829254,
0.04178512096405029,
0.054343268275260925,
0.002559301210567355,
0.023142006248235703,
0.01876436173915863,
0.020943567156791687,
-0.013290546834468842,
0.015240703709423542,
0.01870008558034897,
-0.04439222812652588,
-0.05054905265569687,
0.026684649288654327,
0.0068688164465129375,
0.07552392035722733,
-0.003249791916459799,
-0.02429630421102047,
-0.07485148310661316,
0.038774315267801285,
-0.03610477223992348,
-0.010534374974668026,
-0.04094572737812996,
0.02417944371700287,
-0.050042636692523956,
-0.04251936450600624,
0.08125070482492447,
-0.020765122026205063,
-0.0400061160326004,
0.005783812142908573,
0.03519745171070099,
0.015442677773535252,
0.010240830481052399,
0.010658088140189648,
0.09718926250934601,
-0.016235463321208954,
-0.01792641170322895,
-0.011017984710633755,
-0.004419829696416855,
0.02775454707443714,
-0.043608181178569794,
0.023047562688589096,
-0.038144055753946304,
-0.010156919248402119,
0.011406336911022663,
0.024807455018162727,
0.055709946900606155,
-0.08161155879497528,
0.0020149294286966324,
-0.30467888712882996,
0.005822444334626198,
0.06310965865850449,
0.02365422248840332,
0.06866363435983658,
-0.01255049742758274,
0.04216320440173149,
0.024348042905330658,
-0.0050493571907281876,
-0.04298732802271843,
0.0260655228048563,
0.04642109572887421,
-0.04793058708310127,
-0.026992052793502808,
-0.012933720834553242,
0.051204025745391846,
0.0757756158709526,
-0.041685137897729874,
0.025210637599229813,
0.03842839226126671,
-0.011964220553636551,
-0.009465881623327732,
-0.027330318465828896,
0.05874476581811905,
0.0651823878288269,
-0.03374313935637474,
0.09112421423196793,
-0.07621067017316818,
-0.08477964997291565,
-0.027972092851996422,
0.06996195763349533,
0.005909529514610767,
0.009794446639716625,
-0.0802161768078804,
-0.01831684075295925,
-0.0396595261991024,
-0.04381785914301872,
-0.02684023231267929,
-0.032653842121362686,
-0.0017950466135516763,
-0.07528940588235855,
0.03282683342695236,
0.0021152833942323923,
-0.0005604911129921675,
-0.07722833007574081,
-0.05215996131300926,
0.018098650500178337,
0.00866585411131382,
-0.01793774962425232,
-0.042293988168239594,
0.043503351509571075,
-0.022989245131611824,
0.010156485252082348,
-0.05150380730628967,
0.008088726550340652,
-0.03699708729982376,
-0.013074941001832485,
0.0399918258190155,
-0.09072746336460114,
0.00047177544911392033,
-0.05449976399540901,
-0.025377493351697922,
0.003468362847343087,
-0.05261702090501785,
-0.008900719694793224,
-0.03944651782512665,
0.1010606661438942,
-0.0752420499920845,
0.060334742069244385,
0.005868895445019007,
0.026169462129473686,
0.08967876434326172,
-0.0875248908996582,
0.004913072567433119,
0.04499756172299385,
0.05496196076273918,
-0.03675899654626846,
-0.002143455669283867,
0.04404639080166817,
0.013190258294343948,
0.07575593143701553,
-0.02896878682076931,
0.09417588263750076,
0.013821250759065151,
0.01094107236713171,
0.018304748460650444,
0.015408992767333984,
0.02339543029665947,
-0.01689547672867775,
-0.03019552491605282,
-0.05004136636853218,
-0.011282592080533504,
-0.062370628118515015,
-0.04840867966413498,
0.023860877379775047,
0.014892720617353916,
-0.2727500796318054,
0.004920192062854767,
0.006468499545007944,
0.029197340831160545,
0.0022762217558920383,
-0.0043997266329824924,
0.011447694152593613,
-0.025004342198371887,
-0.045630134642124176,
0.057990383356809616,
0.005203376058489084,
0.031667351722717285,
0.03972619026899338,
-0.004410357214510441,
-0.02937456965446472,
0.019198110327124596,
-0.011624926701188087,
-0.02677372470498085,
-0.0182980727404356,
-0.06208745017647743,
0.006085142958909273,
-0.02300523780286312,
0.11240088939666748,
-0.02025063894689083,
0.00713864341378212,
0.020622698590159416,
0.005438494961708784,
0.020412715151906013,
-0.037169452756643295,
-0.010386218316853046,
0.008196823298931122,
0.02049604244530201,
0.06674841791391373,
-0.02858099155128002,
0.018419558182358742,
0.032440681010484695,
-0.025877995416522026,
-0.05938832834362984,
-0.0013327572960406542,
0.0334748737514019,
-0.00175864074844867,
-0.030012622475624084,
-0.058456532657146454,
-0.038447532802820206,
0.07561802864074707,
-0.016880419105291367,
0.010569932870566845,
-0.002209536964073777,
0.018589423969388008,
-0.042626310139894485,
-0.04054682329297066,
-0.015955260023474693,
0.04688165709376335,
0.029878826811909676,
0.05036550387740135,
-0.001543475897051394,
-0.005408527795225382,
-0.05002773553133011,
-0.024098699912428856,
0.01516733318567276,
-0.0118585005402565,
0.031800348311662674,
-0.022212322801351547,
0.02961568906903267,
-0.0035738868173211813
] |
PMID:8234 | Intracellular acid-base heterogeneity in nucleated avian erythrocytes. | 1. Intracellular hydrogen ion activity, [H+]i, was extimated in human erythrocytes and in nucleated avian erythrocytes from measurements of the distribution of ammonia and 5,5'-dimethyloxazolidine-2,4'-dione (DMO) between intracellular and extracellular fluid. 2. In human erythrocytes there was no difference between values for [H+]i derived from measurements of either DMO or ammonia. 3. In avian erythrocytes, [H+]i(ammonia) was consistently greater than [H+]i(DMO), indicating significant acid-base heterogeneity of the intracellular water. The degree of heterogeneity was assessed by reference to a theoretical model of two compartments of equal size. 4. Experiments with nuclei isolated from avian erythrocytes suggested that DMO is not bound to nucleoproteins, and that the nucleus may be more acidic than the cytoplasm. | Intracellular acid-base heterogeneity in nucleated avian erythrocytes. 1. Intracellular hydrogen ion activity, [H+]i, was extimated in human erythrocytes and in nucleated avian erythrocytes from measurements of the distribution of ammonia and 5,5'-dimethyloxazolidine-2,4'-dione (DMO) between intracellular and extracellular fluid. 2. In human erythrocytes there was no difference between values for [H+]i derived from measurements of either DMO or ammonia. 3. In avian erythrocytes, [H+]i(ammonia) was consistently greater than [H+]i(DMO), indicating significant acid-base heterogeneity of the intracellular water. The degree of heterogeneity was assessed by reference to a theoretical model of two compartments of equal size. 4. Experiments with nuclei isolated from avian erythrocytes suggested that DMO is not bound to nucleoproteins, and that the nucleus may be more acidic than the cytoplasm. | [
-0.040991995483636856,
-0.04745383560657501,
-0.017883675172924995,
-0.01764625497162342,
0.04699249938130379,
0.030369916930794716,
0.06340058147907257,
0.018352258950471878,
0.024417733773589134,
0.0179162435233593,
0.010314074344933033,
-0.10103060305118561,
-0.013169088400900364,
-0.011747701093554497,
-0.0437529981136322,
-0.0194931048899889,
-0.05433136597275734,
-0.030812788754701614,
-0.09854491055011749,
0.025249144062399864,
-0.008432266302406788,
-0.04072393849492073,
0.03276398032903671,
-0.0010750510264188051,
0.04003070667386055,
0.023167969658970833,
-0.0053924801759421825,
0.02660641074180603,
-0.04088016226887703,
-0.21043062210083008,
0.033789411187171936,
-0.01852727308869362,
0.010248244740068913,
-0.010143928229808807,
-0.08037418872117996,
0.023611141368746758,
-0.0030000568367540836,
-0.025914045050740242,
-0.05093071982264519,
0.05376944690942764,
0.03963185101747513,
-0.03713507950305939,
-0.025332840159535408,
0.04620928317308426,
-0.0009621146600693464,
0.009265872649848461,
-0.038459595292806625,
0.03644615411758423,
0.037269849330186844,
0.02146102674305439,
0.0159946009516716,
0.013278904370963573,
-0.0016392209799960256,
0.04082070663571358,
0.0035759261809289455,
0.004518099129199982,
0.032426636666059494,
0.07422738522291183,
0.02122477814555168,
-0.04246753454208374,
-0.020134340971708298,
0.03664225712418556,
-0.13491395115852356,
0.07490267604589462,
0.10925603657960892,
-0.011573058553040028,
-0.008575956337153912,
-0.0417807474732399,
0.03046826831996441,
0.009609564207494259,
-0.04904903471469879,
0.01949779875576496,
0.010522888042032719,
0.024243352934718132,
0.015086204744875431,
-0.014125294052064419,
-0.002573892939835787,
-0.004979358054697514,
-0.002555797342211008,
-0.04919949173927307,
-0.04759711027145386,
-0.05603713542222977,
-0.019191017374396324,
0.02039192058146,
0.0005208306247368455,
0.026510531082749367,
-0.028280774131417274,
-0.023673953488469124,
-0.03004559874534607,
-0.003495255019515753,
0.012448770925402641,
-0.014851830899715424,
-0.04402695223689079,
0.04679492115974426,
-0.07192323356866837,
0.09360270947217941,
0.06184713542461395,
-0.044736407697200775,
0.01665511168539524,
0.3984227478504181,
-0.013225930742919445,
0.029365312308073044,
-0.022993315011262894,
-0.02459689974784851,
0.015840008854866028,
-0.0020267001818865538,
-0.02248971164226532,
-0.03619450330734253,
-0.007449704688042402,
0.060713108628988266,
0.029400555416941643,
0.039382204413414,
0.038300771266222,
-0.08546534925699234,
-0.0018168380483984947,
-0.016330743208527565,
0.029489144682884216,
-0.028476528823375702,
0.011911066249012947,
0.0414198599755764,
-0.032789990305900574,
-0.010432498529553413,
0.006494658067822456,
-0.0118044288828969,
0.09466808289289474,
0.02049928344786167,
-0.038331497460603714,
0.06078705936670303,
0.0058118426240980625,
0.016740921884775162,
0.07305411994457245,
0.05065575987100601,
-0.011685685254633427,
-0.021415241062641144,
0.039489734917879105,
0.025787396356463432,
0.025354038923978806,
-0.04868510365486145,
0.05096501484513283,
-0.020251208916306496,
-0.0484677217900753,
-0.04585615172982216,
-0.06403195112943649,
-0.06690497696399689,
-0.05661173164844513,
0.09122400730848312,
0.006909128278493881,
0.033859945833683014,
0.007937105372548103,
-0.0207536518573761,
0.014835993759334087,
0.07774435728788376,
0.008402046747505665,
0.046887509524822235,
-0.01552579365670681,
0.016888398677110672,
-0.04234486445784569,
0.01056712120771408,
-0.010362702421844006,
-0.03326476365327835,
-0.030419738963246346,
-0.0063901664689183235,
-0.003975146450102329,
0.13123328983783722,
0.009674805216491222,
0.019429918378591537,
-0.004212066996842623,
0.004266870208084583,
0.007465119939297438,
0.013850036077201366,
-0.0005808889982290566,
-0.005551605951040983,
0.029863230884075165,
-0.01223706640303135,
0.010543404147028923,
-0.006978579331189394,
-0.013931532390415668,
0.027930105105042458,
0.022183595225214958,
-0.03703616186976433,
0.010281404480338097,
-0.012953187339007854,
-0.035541828721761703,
0.03705583140254021,
0.05789743736386299,
-0.06426204741001129,
-0.07699040323495865,
0.021916085854172707,
0.02982715331017971,
0.04755493253469467,
-0.022259334102272987,
0.03339661285281181,
-0.0016773196402937174,
0.026963606476783752,
-0.006931425537914038,
-0.09340011328458786,
0.010347193107008934,
0.019750451669096947,
0.0063531724736094475,
0.01449738908559084,
0.0623616948723793,
0.01121032889932394,
0.007774276193231344,
0.03888647258281708,
-0.010609467513859272,
0.017832908779382706,
0.014756586402654648,
-0.010962920263409615,
0.03182210400700569,
-0.030534403398633003,
-0.037428803741931915,
-0.018467426300048828,
-0.08835985511541367,
0.034397322684526443,
-0.06291666626930237,
0.022089488804340363,
0.03462184593081474,
0.061179645359516144,
0.04864724725484848,
-0.013790852390229702,
0.03268064185976982,
-0.12744079530239105,
-0.06398849189281464,
-0.27817460894584656,
-0.04623546451330185,
-0.022399580106139183,
-0.025669114664196968,
0.021625179797410965,
0.013795197010040283,
0.06248997896909714,
-0.03367255628108978,
0.06949426978826523,
-0.032424163073301315,
0.007627792190760374,
0.037350453436374664,
-0.03213132545351982,
-0.08290380984544754,
0.012195127084851265,
0.028119096532464027,
0.002278874395415187,
-0.01824057847261429,
0.03553701937198639,
0.029928065836429596,
0.04467748478055,
0.017171598970890045,
-0.035187311470508575,
0.07331444323062897,
0.06191002205014229,
-0.07994623482227325,
0.09436949342489243,
0.012468577362596989,
0.036427855491638184,
0.012841582298278809,
0.005681932438164949,
0.06735065579414368,
0.010059104301035404,
-0.04647087678313255,
-0.04229874163866043,
-0.028042839840054512,
-0.04748329147696495,
-0.03635893762111664,
0.0019666559528559446,
-0.041715651750564575,
-0.03188087418675423,
0.017333708703517914,
-0.02671550028026104,
-0.07935713231563568,
-0.028865288943052292,
0.005759861785918474,
-0.022824818268418312,
0.024442030116915703,
-0.016811484470963478,
-0.07911737263202667,
0.08066251873970032,
-0.04440879449248314,
-0.008994657546281815,
-0.06020105257630348,
0.03548773378133774,
0.009591017849743366,
-0.03293741121888161,
-0.01851903647184372,
-0.0990218073129654,
-0.027036581188440323,
0.010301334783434868,
0.013929458335042,
-0.018724270164966583,
0.004005055874586105,
0.01372150331735611,
0.0034652322065085173,
0.0433899387717247,
0.030026931315660477,
0.1040259599685669,
-0.022790277376770973,
0.06862581521272659,
0.10010389983654022,
-0.04602755606174469,
0.07183697819709778,
0.05720997974276543,
0.011721659451723099,
-0.06830202043056488,
-0.007122341077774763,
-0.0008805837132968009,
0.013923576101660728,
0.03426500782370567,
-0.08144396543502808,
0.08850246667861938,
-0.014989553019404411,
-0.036496058106422424,
0.04546462744474411,
0.01304383110255003,
0.026284687221050262,
0.02681025303900242,
-0.0017041335813701153,
0.02615731582045555,
-0.00770998140797019,
-0.029541930183768272,
-0.058193717151880264,
0.009735138155519962,
0.019559914246201515,
-0.25595682859420776,
0.0007333845132961869,
-0.002936573000624776,
-0.00045610524830408394,
-0.09024149179458618,
0.010732614435255527,
0.00622138986364007,
-0.02245168201625347,
-0.02188197337090969,
0.014139538630843163,
0.024099299684166908,
-0.0004748983192257583,
0.029460271820425987,
0.022348687052726746,
-0.02954571135342121,
0.06647012382745743,
0.03470195457339287,
-0.03198399767279625,
-0.0016559894429519773,
-0.023651599884033203,
0.02081809565424919,
-0.010710477828979492,
0.17470458149909973,
-0.02259630337357521,
0.012856368906795979,
0.030511880293488503,
-0.025360051542520523,
0.028485074639320374,
-0.07319209724664688,
0.01054453756660223,
0.014691613614559174,
0.03934411332011223,
0.05104267597198486,
-0.009937597438693047,
0.03585835173726082,
-0.011282254941761494,
0.002019696170464158,
0.000638918369077146,
-0.01242025475949049,
0.001177697442471981,
-0.003102485090494156,
0.024405479431152344,
-0.08040549606084824,
0.0006148378597572446,
0.04158990830183029,
-0.027855591848492622,
0.016577458009123802,
-0.038035351783037186,
0.061523761600255966,
-0.07323292642831802,
-0.04695151373744011,
-0.0573580302298069,
0.03576174005866051,
0.04182909429073334,
0.040782682597637177,
-0.0197291262447834,
0.004355026874691248,
-0.02823430672287941,
-0.026797499507665634,
-0.006869731470942497,
0.05746454373002052,
0.0036077017430216074,
-0.0206803809851408,
0.019568225368857384,
0.013743888586759567
] |
PMID:8236 | Telephone management of poisonings using syrup of ipecac. | Seven hundred and seventy-six cases were studied during a six-month period to see if induction of emesis could be successfully managed at home by telephone. Emesis was successful in 98.8% of cases. In 6.7% of all cases, symptoms were found at 4-hour follow-up that were referrable to the ingestion, but all were considered to be of minor consequence. No complications of vomiting occurred. Twenty-four hour follow-up investigation indicated no significant complications of induction of emesis or complications from managing the patient by telephone. It is our conclusion that, with appropriate telephone supervision, home-induced emesis of ingestions expected to produce mild to moderate symptoms is as effective as emergency room or physician office management of cases. Furthermore, the absence of adverse affects of complications arising from the induction of emesis at home in our cases confirms that this form of management is quite safe. | Telephone management of poisonings using syrup of ipecac. Seven hundred and seventy-six cases were studied during a six-month period to see if induction of emesis could be successfully managed at home by telephone. Emesis was successful in 98.8% of cases. In 6.7% of all cases, symptoms were found at 4-hour follow-up that were referrable to the ingestion, but all were considered to be of minor consequence. No complications of vomiting occurred. Twenty-four hour follow-up investigation indicated no significant complications of induction of emesis or complications from managing the patient by telephone. It is our conclusion that, with appropriate telephone supervision, home-induced emesis of ingestions expected to produce mild to moderate symptoms is as effective as emergency room or physician office management of cases. Furthermore, the absence of adverse affects of complications arising from the induction of emesis at home in our cases confirms that this form of management is quite safe. | [
0.01578616537153721,
0.005057926755398512,
0.047985196113586426,
-0.02317732572555542,
-0.007837293669581413,
-0.0622066892683506,
0.0501018650829792,
0.08573928475379944,
-0.06990521401166916,
-0.011829201132059097,
-0.06367570161819458,
-0.003561994293704629,
-0.020759090781211853,
-0.04266864433884621,
-0.010984954424202442,
0.02625688910484314,
0.009891974739730358,
-0.04150775820016861,
-0.037620194256305695,
0.0642876997590065,
-0.027711715549230576,
0.002336124423891306,
-0.02645002491772175,
0.010188029147684574,
0.02632599137723446,
0.007926322519779205,
0.008697331883013248,
7.707984082117036e-7,
-0.07133698463439941,
-0.14223605394363403,
-0.008655468001961708,
-0.025868577882647514,
0.021813763305544853,
0.015202444046735764,
-0.03382352739572525,
-0.004857330583035946,
-0.032403554767370224,
0.01284997258335352,
-0.024956438690423965,
-0.008823366835713387,
-0.0073362006805837154,
0.05502878874540329,
0.03297371044754982,
-0.058613017201423645,
-0.020645078271627426,
-0.058983925729990005,
0.015883350744843483,
-0.02132158726453781,
0.08520052582025528,
0.02087816223502159,
0.040709126740694046,
-0.004838974680751562,
0.022810082882642746,
0.0505041629076004,
0.013897682540118694,
0.01717134192585945,
0.002905749948695302,
-0.046718623489141464,
-0.023844728246331215,
0.024281416088342667,
-0.006844583433121443,
0.03301212936639786,
-0.13702534139156342,
0.08870566636323929,
0.02258601225912571,
-0.0546589232981205,
-0.013845035806298256,
0.020656485110521317,
0.08961089700460434,
0.044534120708703995,
-0.039215948432683945,
0.04310479387640953,
-0.02595113404095173,
0.070598304271698,
-0.03515395149588585,
0.0054820841178298,
0.052817802876234055,
-0.01439310610294342,
-0.050641436129808426,
0.012938686646521091,
0.022456331178545952,
-0.028701817616820335,
0.026762589812278748,
-0.0827689841389656,
0.02163996361196041,
-0.04295602813363075,
0.01851006969809532,
0.042102307081222534,
0.00011484411515993997,
0.0380319207906723,
-0.001379256951622665,
-0.001316882437095046,
-0.053550299257040024,
0.013675399124622345,
-0.005777812097221613,
-0.01776755601167679,
-0.007765698712319136,
-0.017386797815561295,
-0.043276336044073105,
0.37585726380348206,
-0.03477417677640915,
0.02865540236234665,
-0.052643999457359314,
-0.04872913658618927,
0.019808102399110794,
-0.03775723651051521,
0.006116574630141258,
-0.007361084222793579,
-0.011758729815483093,
0.005293952766805887,
0.0158764086663723,
0.00409073056653142,
0.08013520389795303,
-0.012940024957060814,
-0.0035771243274211884,
0.1165546104311943,
0.06381500512361526,
-0.014187781140208244,
0.0008954593213275075,
0.0032274937257170677,
-0.017846418544650078,
0.03481670096516609,
0.027486108243465424,
-0.018373239785432816,
0.07523205876350403,
0.008441155776381493,
0.04426851123571396,
0.07782664895057678,
-0.01140452828258276,
0.008296863175928593,
0.0404447540640831,
-0.02471151202917099,
0.010118012316524982,
0.014130090363323689,
0.0170640479773283,
-0.00009981072071241215,
0.08345075696706772,
-0.008825321681797504,
0.032520633190870285,
0.008682706393301487,
-0.006237519904971123,
-0.1059260293841362,
-0.003173177596181631,
-0.08441637456417084,
-0.03165236487984657,
0.07097084820270538,
0.035291384905576706,
-0.024732504040002823,
-0.04774683341383934,
-0.07709294557571411,
-0.010416320525109768,
0.019773278385400772,
0.013750607147812843,
-0.007651520427316427,
-0.007857434451580048,
0.02935367450118065,
0.035271890461444855,
0.02329818345606327,
0.025738410651683807,
0.03044794872403145,
-0.02730308473110199,
-0.0074388994835317135,
-0.03146747872233391,
0.06747996807098389,
-0.054562509059906006,
-0.08412560075521469,
-0.05613042414188385,
-0.02160361036658287,
0.0063191675581038,
-0.025950171053409576,
0.018844788894057274,
0.00039381819078698754,
0.02573101967573166,
0.030621862038969994,
-0.01838632859289646,
-0.0030770362354815006,
0.02822072058916092,
0.00696410471573472,
0.06972875446081161,
0.03207765147089958,
0.04486430436372757,
0.0038913532625883818,
0.08128722757101059,
0.014291959814727306,
0.0013833416160196066,
-0.014325112104415894,
-0.006912951823323965,
0.018133023753762245,
0.10007210820913315,
0.019954422488808632,
-0.03910418227314949,
0.014391520991921425,
-0.08631572872400284,
0.018833322450518608,
-0.008103916421532631,
-0.03795161843299866,
-0.03812267631292343,
-0.04485716670751572,
0.022275786846876144,
0.008667905814945698,
0.021620478481054306,
-0.002320291008800268,
0.06812337785959244,
0.04462367296218872,
0.04564651846885681,
0.045350100845098495,
0.003876577829942107,
0.0013976235641166568,
0.06552119553089142,
0.022572215646505356,
-0.009094782173633575,
0.053120024502277374,
0.09712095558643341,
-0.005795062053948641,
-0.011714118532836437,
0.06698499619960785,
-0.011579262092709541,
0.02525627799332142,
0.07039141654968262,
0.017923545092344284,
0.02214137651026249,
0.04997734725475311,
-0.022718895226716995,
-0.3262633681297302,
-0.05195886641740799,
0.026690280064940453,
-0.031148646026849747,
-0.037815358489751816,
-0.041298482567071915,
0.04047563672065735,
-0.08677493035793304,
-0.02202926017343998,
-0.0802215039730072,
0.062369439750909805,
0.02005758322775364,
0.023504894226789474,
-0.015643848106265068,
0.037204891443252563,
0.003261842764914036,
0.04141146317124367,
-0.03654031082987785,
0.012962628155946732,
0.029386086389422417,
-0.06384104490280151,
-0.020895536988973618,
0.0361747220158577,
0.011806379072368145,
0.015169559977948666,
-0.010212907567620277,
0.11437387019395828,
-0.03296495974063873,
-0.013434374704957008,
-0.07386850565671921,
0.03225509077310562,
0.013696377165615559,
0.01724371314048767,
-0.08209376037120819,
0.02838451974093914,
-0.03702770918607712,
-0.030955374240875244,
-0.012353113852441311,
-0.034387052059173584,
-0.0259106382727623,
-0.054622944444417953,
0.008051849901676178,
0.018585234880447388,
-0.042075712233781815,
-0.08759605139493942,
0.002861055778339505,
-0.03737182915210724,
0.004617250524461269,
-0.04270284250378609,
0.0009364166762679815,
0.03856102004647255,
0.006163895595818758,
0.0038696229457855225,
0.04663338139653206,
-0.017187412828207016,
-0.04173486679792404,
-0.0687219500541687,
0.03858373314142227,
-0.10707715898752213,
-0.015801062807440758,
-0.010561949573457241,
0.00851710420101881,
0.03166983649134636,
-0.006714974995702505,
-0.009223700501024723,
-0.002665979554876685,
0.04496390372514725,
0.007621892262250185,
0.06712227314710617,
0.022740382701158524,
-0.04202686622738838,
0.14347636699676514,
-0.09427551925182343,
-0.015369949862360954,
0.013971803709864616,
0.04031083732843399,
0.02808910235762596,
-0.03015930950641632,
-0.09574538469314575,
0.014790147542953491,
-0.02227131277322769,
0.010674337856471539,
0.003510626731440425,
0.06966704875230789,
-0.05580219253897667,
-0.010180879384279251,
-0.023555712774395943,
0.03835047408938408,
-0.009871658869087696,
0.004676236771047115,
0.02138338051736355,
0.0009723415714688599,
-0.04990391433238983,
0.031190628185868263,
0.036132074892520905,
0.03095567412674427,
-0.25966212153434753,
0.025758685544133186,
-0.02956203557550907,
0.02851119078695774,
-0.013712570071220398,
-0.01702277548611164,
0.0018383129499852657,
-0.007559013087302446,
-0.01218370534479618,
-0.013288462534546852,
0.00394299766048789,
-0.02001086063683033,
0.1280461698770523,
0.028190551325678825,
0.03731266409158707,
-0.014349015429615974,
0.009448359720408916,
-0.017269734293222427,
-0.01649019867181778,
-0.06671230494976044,
0.03396870195865631,
-0.017744559794664383,
0.11499146372079849,
0.024395093321800232,
-0.056999459862709045,
0.04680480808019638,
0.05261313542723656,
0.045392051339149475,
0.007054523099213839,
0.0018212911672890186,
0.007482886780053377,
-0.004667591769248247,
0.041525546461343765,
-0.012522838078439236,
0.06509461998939514,
-0.11160620301961899,
-0.04724454507231712,
0.025383947417140007,
-0.022773422300815582,
-0.028206193819642067,
-0.10206130146980286,
-0.006002127192914486,
-0.01769162341952324,
-0.04300627112388611,
0.003626651829108596,
-0.0016823741607367992,
-0.044468287378549576,
-0.05466756969690323,
-0.024393368512392044,
0.003689353819936514,
-0.01587214507162571,
-0.09756282716989517,
0.0662728026509285,
0.017041532322764397,
-0.003689196426421404,
0.005158454645425081,
-0.03298904001712799,
0.008119171485304832,
-0.033567316830158234,
0.018322549760341644,
-0.007039949763566256,
0.025988860055804253,
0.006418978795409203,
0.07169555127620697,
0.019384589046239853
] |
PMID:8278 | Cyclic AMP and cyclic GMP in epidermal physiology and pathophysiology. | The second messengers cyclic AMP and cyclic GMP in several organs appear to coordinate those molecular events which are responsible for specialized organ function. As a result of balanced cell proliferation and specialization, epidermis functions by terminal specialization which provides a barrier between man and environment. Since the epidermal component of psoriasis is a classic example of deranged epidermal homeostasis, which has a low level of cyclic AMP and a high level of cyclic GMP, it seems reasonable that rebalancing these cyclic nucleotides might ultimately be a safe and effective therapy for psoriasis and other proliferative skin diseases. | Cyclic AMP and cyclic GMP in epidermal physiology and pathophysiology. The second messengers cyclic AMP and cyclic GMP in several organs appear to coordinate those molecular events which are responsible for specialized organ function. As a result of balanced cell proliferation and specialization, epidermis functions by terminal specialization which provides a barrier between man and environment. Since the epidermal component of psoriasis is a classic example of deranged epidermal homeostasis, which has a low level of cyclic AMP and a high level of cyclic GMP, it seems reasonable that rebalancing these cyclic nucleotides might ultimately be a safe and effective therapy for psoriasis and other proliferative skin diseases. | [
-0.01391295064240694,
-0.008423171006143093,
0.023562584072351456,
-0.004379903431981802,
0.010140242986381054,
-0.05242311209440231,
0.06583186239004135,
0.023203877732157707,
-0.06292664259672165,
-0.018303891643881798,
0.01795634813606739,
-0.09113254398107529,
-0.03317123278975487,
0.04873023182153702,
0.002722339238971472,
0.01137632131576538,
-0.007826127111911774,
0.0484461672604084,
0.01804986596107483,
0.019404541701078415,
0.019615503028035164,
0.003755078883841634,
0.01441810093820095,
0.010310210287570953,
0.023909326642751694,
-0.0237042885273695,
-0.0022976212203502655,
-0.0018821326084434986,
-0.05012044310569763,
-0.16783156991004944,
0.007001272402703762,
0.08153349161148071,
-0.05860293656587601,
-0.016073543578386307,
-0.01016184315085411,
-0.07847725600004196,
-0.05501815676689148,
0.036118730902671814,
-0.01840406283736229,
0.09115466475486755,
-0.008622675202786922,
0.012617317959666252,
-0.027185646817088127,
0.015351617708802223,
0.0798613503575325,
-0.03088849037885666,
-0.06946004927158356,
-0.010630102828145027,
0.07190299779176712,
-0.05201353877782822,
0.06450628489255905,
0.005367312114685774,
0.001674169092439115,
0.03814316540956497,
-0.01644982397556305,
0.014806821942329407,
0.008129699155688286,
0.05803348124027252,
-0.014382172375917435,
0.027457479387521744,
-0.0003442357701715082,
0.04827316850423813,
-0.10689108818769455,
0.0637042298913002,
0.052254535257816315,
0.003315129317343235,
0.00769437151029706,
-0.01704925298690796,
-0.010296785272657871,
0.05051644146442413,
-0.06361589580774307,
0.02481263130903244,
0.005115118343383074,
-0.029664844274520874,
-0.012717225588858128,
-0.007597132585942745,
-0.048615992069244385,
-0.019812529906630516,
0.010730680078268051,
0.0489485040307045,
0.016862617805600166,
0.016297664493322372,
0.021090541034936905,
-0.0888482928276062,
0.02066415175795555,
-0.037883710116147995,
-0.009194408543407917,
-0.07176049798727036,
-0.03618452697992325,
0.03423989564180374,
-0.02093341015279293,
-0.01990935020148754,
-0.005070717539638281,
-0.0467555858194828,
-0.05256304889917374,
0.007181069813668728,
0.0015853028744459152,
-0.007293948903679848,
-0.003544857259839773,
0.35222575068473816,
-0.026295995339751244,
-0.046988826245069504,
-0.07740283757448196,
0.031187590211629868,
0.02792150154709816,
-0.01442223135381937,
-0.006531394086778164,
-0.00653829937800765,
-0.08577270805835724,
0.048854317516088486,
0.0354384109377861,
0.0081770159304142,
0.005750168580561876,
-0.01917308010160923,
0.013107097707688808,
-0.06117530167102814,
0.01837744750082493,
0.034509144723415375,
-0.023284820839762688,
0.018188010901212692,
-0.007489305920898914,
-0.04285193234682083,
0.007846425287425518,
-0.013657952658832073,
0.07878176867961884,
0.03335696831345558,
0.031709473580121994,
0.0768471509218216,
0.0904305949807167,
-0.02142886258661747,
0.02742219716310501,
0.029334818944334984,
-0.06579173356294632,
0.038863405585289,
0.015646278858184814,
0.005613797809928656,
0.011659598909318447,
-0.07200293987989426,
0.0015876202378422022,
0.022030284628272057,
-0.022860638797283173,
0.02153860591351986,
-0.037417542189359665,
-0.039883121848106384,
0.024094536900520325,
0.1385190784931183,
-0.0030873140785843134,
0.033038344234228134,
-0.011458523571491241,
0.07757554203271866,
-0.047203030437231064,
0.021266043186187744,
0.029597388580441475,
0.019538363441824913,
0.005314347334206104,
0.051166847348213196,
-0.019137080758810043,
-0.05135659873485565,
0.043744053691625595,
-0.05088791251182556,
-0.06274965405464172,
0.0006672079907730222,
0.02324947901070118,
0.11261825263500214,
0.04928049445152283,
-0.02160903811454773,
-0.04335763677954674,
-0.01970227248966694,
0.012464186176657677,
-0.06041432544589043,
-0.02779780700802803,
0.0019438638119027019,
0.0001063282325048931,
-0.050877463072538376,
-0.027303606271743774,
0.023407911881804466,
-0.023866552859544754,
0.0029695401899516582,
-0.025906315073370934,
-0.014123991131782532,
0.05921239033341408,
-0.0003198813064955175,
-0.02177293412387371,
-0.009160132147371769,
0.03697679564356804,
-0.0177213903516531,
-0.04824355989694595,
0.023898938670754433,
0.05128641054034233,
0.004514008294790983,
-0.044051140546798706,
-0.0013848947128280997,
-0.05135258659720421,
0.02527773566544056,
-0.026554228737950325,
-0.049263957887887955,
-0.03969357907772064,
-0.03200521692633629,
-0.07394308596849442,
0.025348437950015068,
0.017602892592549324,
0.009152594953775406,
0.0436771884560585,
0.04452762007713318,
-0.025464879348874092,
0.05547318980097771,
-0.027819432318210602,
0.023109732195734978,
0.04883652552962303,
0.009503046050667763,
-0.01876041106879711,
-0.0035101890098303556,
0.008128817193210125,
-0.043924011290073395,
-0.02059347927570343,
0.021961480379104614,
-0.004955447744578123,
0.06862747669219971,
0.07795845717191696,
-0.011613540351390839,
-0.02461394853889942,
0.05645265057682991,
-0.03142459690570831,
-0.3015202283859253,
-0.03974523767828941,
0.007487607654184103,
-0.019916025921702385,
0.04365414381027222,
0.004212700296193361,
0.057298291474580765,
-0.020638003945350647,
0.003959968686103821,
-0.058132510632276535,
0.08940769731998444,
0.08119813352823257,
-0.07230167835950851,
-0.030527252703905106,
-0.07499369978904724,
0.05808676779270172,
0.008844318799674511,
-0.052659060806035995,
0.029365239664912224,
-0.002998246578499675,
0.03977666795253754,
0.027771491557359695,
0.02768382430076599,
-0.041670799255371094,
-0.014618533663451672,
0.0007391948020085692,
0.09146299958229065,
0.006538888905197382,
-0.01844499446451664,
0.005332641769200563,
0.016860241070389748,
-0.006493137683719397,
-0.02163967303931713,
-0.0612151212990284,
-0.02810909040272236,
-0.02771308831870556,
-0.03047693334519863,
-0.033576127141714096,
-0.023310810327529907,
-0.010657928884029388,
-0.05562414973974228,
0.021044045686721802,
-0.05022028833627701,
0.011224714107811451,
-0.08674619346857071,
-0.025691935792565346,
0.017025263980031013,
0.0471566878259182,
0.060602787882089615,
-0.07547803223133087,
0.020646309480071068,
-0.02760154753923416,
0.004603878129273653,
-0.02775692194700241,
0.041469648480415344,
-0.008593717589974403,
-0.01676570624113083,
-0.01906067132949829,
-0.12189874053001404,
0.020582087337970734,
0.021766014397144318,
0.09538678079843521,
0.028612909838557243,
0.012856445275247097,
0.021012205630540848,
0.019963771104812622,
0.03497274965047836,
-0.02630612440407276,
0.052207667380571365,
0.016838692128658295,
0.02342921681702137,
0.1276503950357437,
-0.027869651094079018,
0.013833819888532162,
0.018077921122312546,
0.008663268759846687,
0.02847861498594284,
-0.021707851439714432,
-0.062662273645401,
-0.03586277738213539,
0.001631561666727066,
-0.06270257383584976,
0.04250873252749443,
0.012336750514805317,
-0.02949850633740425,
-0.015647681429982185,
0.03920686990022659,
-0.018374618142843246,
0.01974557153880596,
-0.08042605221271515,
-0.03213498368859291,
0.04527183994650841,
-0.022941524162888527,
-0.022183669731020927,
0.0036581435706466436,
0.015527090057730675,
-0.2909291684627533,
0.012162434868514538,
0.0026793263386934996,
-0.01027525682002306,
-0.043769821524620056,
0.0647861436009407,
0.02342989295721054,
-0.0032132675405591726,
-0.08292502909898758,
0.0356140099465847,
0.08021631836891174,
0.029384298250079155,
0.06383827328681946,
0.08724531531333923,
0.04183695465326309,
0.04852684214711189,
0.05911751836538315,
-0.057903923094272614,
-0.022124119102954865,
-0.01572854071855545,
0.01964089460670948,
-0.010863645933568478,
0.1755380928516388,
-0.04541802778840065,
0.038929108530282974,
0.037201132625341415,
0.01485185045748949,
0.007839027792215347,
0.02693047560751438,
0.015366679057478905,
-0.00020594683883246034,
0.014275251887738705,
0.03270501643419266,
-0.0004477805632632226,
0.0623445026576519,
-0.0013670820044353604,
0.018410520628094673,
0.01746152713894844,
0.01409572921693325,
-0.014186633750796318,
-0.014389238320291042,
-0.001308246050029993,
-0.021330785006284714,
-0.01200658269226551,
0.07189597934484482,
-0.11625827103853226,
-0.09145855158567429,
-0.0363193154335022,
0.058664072304964066,
0.022623222321271896,
-0.005601862911134958,
-0.0060448963195085526,
0.048839129507541656,
0.009969614446163177,
0.005431888159364462,
-0.03397054225206375,
-0.027033545076847076,
-0.01538130547851324,
0.01970268040895462,
0.019201377406716347,
0.008871935307979584,
-0.011395448818802834,
-0.08129575848579407,
0.07218552380800247,
0.03182506561279297
] |
PMID:8283 | Pleural fluid pH in parapneumonic effusions. | The pH and carbon dioxide tension were measured in 24 consecutive parapneumonic effusions, along with the leukocyte count, leukocytic differential count, and levels of glucose and protein. Three categories of parapneumonic effusions were characterized: (1) empyemas; (2) benign (nonloculated) effusions; and (3) loculated effusions. A pH greater than 7.30 was present in all ten benign effusions, and spontaneous resolution occurred in each case. All ten empyemas and the four loculated effusions had a pH less than 7.30. All four loculated effusions required drainage with a chest tube for resolution. The pH of the pleural fluid alone separated the empyemas and loculated effusions from benign effusions. The early separation of parapneumonic effusions on the basis of the pleural fluid appears useful. If the pH is greater than 7.30, a benign effusion is present, and spontaneous resolution is likely. If the pH is less than 7.30, loculation of the pleural space may occur regardless of whether the effusion fulfills the criteria for empyema. | Pleural fluid pH in parapneumonic effusions. The pH and carbon dioxide tension were measured in 24 consecutive parapneumonic effusions, along with the leukocyte count, leukocytic differential count, and levels of glucose and protein. Three categories of parapneumonic effusions were characterized: (1) empyemas; (2) benign (nonloculated) effusions; and (3) loculated effusions. A pH greater than 7.30 was present in all ten benign effusions, and spontaneous resolution occurred in each case. All ten empyemas and the four loculated effusions had a pH less than 7.30. All four loculated effusions required drainage with a chest tube for resolution. The pH of the pleural fluid alone separated the empyemas and loculated effusions from benign effusions. The early separation of parapneumonic effusions on the basis of the pleural fluid appears useful. If the pH is greater than 7.30, a benign effusion is present, and spontaneous resolution is likely. If the pH is less than 7.30, loculation of the pleural space may occur regardless of whether the effusion fulfills the criteria for empyema. | [
-0.036003585904836655,
-0.05045173317193985,
-0.0019280597334727645,
0.008144455961883068,
0.003317949827760458,
-0.03066752851009369,
0.07504268735647202,
0.014056175015866756,
-0.00548711558803916,
0.05715181306004524,
0.0004005003720521927,
-0.10488910228013992,
-0.022283729165792465,
0.009978369809687138,
-0.030850231647491455,
-0.050840042531490326,
0.005802271421998739,
0.04447297379374504,
-0.08763879537582397,
0.02005869150161743,
0.007362596224993467,
0.03999662399291992,
0.015867656096816063,
0.004948449786752462,
0.05672156810760498,
0.021593786776065826,
0.013584577478468418,
-0.0017269239760935307,
-0.014851157553493977,
-0.15724176168441772,
0.031789928674697876,
-0.007637956645339727,
0.01879001408815384,
0.009653215296566486,
0.0008314510923810303,
-0.010506234131753445,
0.009566775523126125,
0.039349693804979324,
-0.010009012185037136,
0.07861018925905228,
0.003963122144341469,
0.006972483359277248,
-0.012400046922266483,
-0.027038319036364555,
0.036106906831264496,
-0.06271523237228394,
-0.04247162863612175,
0.02550709806382656,
0.1189267486333847,
0.005848994012922049,
-0.00890860054641962,
-0.014355258084833622,
-0.04300534352660179,
0.06595349311828613,
-0.04990405961871147,
-0.05440165847539902,
0.046967923641204834,
-0.006902024615556002,
-0.010029520839452744,
0.014886464923620224,
-0.047501303255558014,
0.036482073366642,
-0.12628400325775146,
0.03800031915307045,
0.065473772585392,
-0.011370371095836163,
-0.05819368362426758,
-0.0448513925075531,
0.02917725034058094,
0.05561695620417595,
-0.017026618123054504,
0.040396250784397125,
-0.027003848925232887,
0.06822874397039413,
0.01759381964802742,
-0.044377364218235016,
-0.0074157388880848885,
-0.024116426706314087,
-0.020657658576965332,
0.02326928824186325,
0.004979182034730911,
0.013062696903944016,
-0.0005102282739244401,
-0.017770228907465935,
-0.014917359687387943,
-0.016649901866912842,
0.004714766051620245,
-0.0005786169203929603,
-0.055578120052814484,
-0.00004321492087910883,
-0.0047378214076161385,
-0.022113334387540817,
-0.07004912197589874,
0.10801394283771515,
0.005242757964879274,
0.0028509688563644886,
0.04080834239721298,
0.011668889783322811,
0.07519414275884628,
0.37857624888420105,
-0.0280791986733675,
-0.03323393315076828,
0.00974927470088005,
-0.03180094435811043,
0.01088990643620491,
-0.029452266171574593,
0.02430594339966774,
-0.015184487216174603,
0.02214447595179081,
0.03787585347890854,
-0.01605920121073723,
-0.005619991570711136,
0.047736428678035736,
-0.022134246304631233,
-0.06732574850320816,
0.00775735080242157,
0.040584661066532135,
0.026370767503976822,
-0.016472144052386284,
0.023832684382796288,
-0.02429475635290146,
-0.05802590399980545,
-0.01429557055234909,
-0.057120081037282944,
0.053438134491443634,
-0.009165043011307716,
0.05772007256746292,
0.05788333714008331,
0.040826696902513504,
0.032858967781066895,
-0.009347367100417614,
0.03278583288192749,
-0.01256546750664711,
-0.008774551562964916,
0.009610096924006939,
-0.06812454015016556,
0.05267873406410217,
0.012518269009888172,
0.030508622527122498,
0.07207397371530533,
-0.07555510848760605,
-0.0818982869386673,
-0.056738100945949554,
-0.08027249574661255,
-0.05029118433594704,
0.12473146617412567,
-0.050501421093940735,
0.021642819046974182,
-0.004314495716243982,
-0.0166494008153677,
-0.02794748730957508,
0.04557694494724274,
0.0023039141669869423,
0.030992325395345688,
0.039276350289583206,
-0.023725084960460663,
-0.00035436314647085965,
0.016113435849547386,
0.007323357276618481,
-0.007250179070979357,
0.0053868480026721954,
-0.046529706567525864,
-0.01483940053731203,
0.07772679626941681,
-0.04735860228538513,
-0.0017300344770774245,
-0.027905579656362534,
-0.0657142624258995,
-0.04094291850924492,
-0.04230748116970062,
0.030801746994256973,
0.022752370685338974,
-0.026287175714969635,
-0.04638019576668739,
-0.0029889473225921392,
0.03819335252046585,
-0.01954576186835766,
-0.00377402501180768,
0.04824475944042206,
0.05100911483168602,
0.009597153402864933,
0.03753333166241646,
-0.0038248486816883087,
-0.02761184237897396,
0.02247004583477974,
-0.07838008552789688,
-0.07484950870275497,
-0.00290030799806118,
0.05493774265050888,
0.03671782836318016,
-0.04516751691699028,
-0.0401013046503067,
-0.059613700956106186,
0.00974299106746912,
0.03805698826909065,
-0.018545564264059067,
0.014785071834921837,
-0.038691338151693344,
-0.006168410647660494,
0.010289224795997143,
0.11833365261554718,
-0.024578439071774483,
0.028318040072917938,
0.07121770828962326,
0.011157190427184105,
0.04428510367870331,
0.012988975271582603,
0.057029157876968384,
-0.05347856879234314,
0.020266693085432053,
-0.05361853539943695,
0.023168768733739853,
-0.0635736733675003,
0.018687812611460686,
-0.0597250871360302,
0.08519531786441803,
0.029310429468750954,
-0.043157462030649185,
0.02039572037756443,
-0.06215222179889679,
0.02218291163444519,
-0.030465858057141304,
0.013480870984494686,
-0.2636094093322754,
0.029793860390782356,
0.050148919224739075,
-0.005090015009045601,
0.11757724732160568,
-0.04791702330112457,
0.025288017466664314,
0.05075862258672714,
0.032901111990213394,
-0.03035443276166916,
0.045939527451992035,
-0.006562838330864906,
-0.08154089748859406,
-0.024990346282720566,
0.03941591829061508,
0.013665299862623215,
0.04867643490433693,
-0.030557768419384956,
0.0037385784089565277,
0.06538087129592896,
0.03754470869898796,
-0.0034656026400625706,
-0.055288586765527725,
0.04051411896944046,
0.004373282194137573,
0.011244595982134342,
0.152610644698143,
-0.07561874389648438,
-0.029632125049829483,
-0.051244135946035385,
0.06414295732975006,
0.008010408841073513,
0.001193020842038095,
-0.041464366018772125,
-0.03043767437338829,
-0.003556196577847004,
-0.010861196555197239,
-0.019578535109758377,
-0.03648098558187485,
-0.014241259545087814,
-0.06582782417535782,
0.044250309467315674,
-0.06901339441537857,
-0.03244013339281082,
-0.048755042254924774,
-0.0011933891801163554,
-0.014881915412843227,
0.11058130115270615,
-0.03433318808674812,
0.030053166672587395,
0.03225986286997795,
-0.002015952719375491,
0.01995353028178215,
-0.06037214770913124,
-0.014512713998556137,
0.04562275484204292,
-0.01040304359048605,
-0.06227628141641617,
-0.10590839385986328,
-0.05473901703953743,
-0.05027499422430992,
-0.03745219483971596,
0.017097845673561096,
-0.0023511305917054415,
0.010690915398299694,
-0.05909809097647667,
0.04731522873044014,
0.0054579139687120914,
0.03989393636584282,
0.014111672528088093,
-0.0004974103649146855,
0.11141572892665863,
-0.07281595468521118,
0.014552285894751549,
0.05364476516842842,
0.031783577054739,
0.010023444890975952,
-0.002120697870850563,
0.000521795591339469,
0.0035007777623832226,
0.027112925425171852,
-0.03949188441038132,
0.022444870322942734,
0.001319092814810574,
-0.017944874241948128,
0.020361075177788734,
-0.02495385706424713,
0.0019792881794273853,
-0.054349951446056366,
-0.048894405364990234,
-0.0071218255907297134,
0.0010106851113960147,
-0.02166455052793026,
0.004111712798476219,
0.016913583502173424,
0.006313820835202932,
-0.31124165654182434,
0.027761928737163544,
0.04720299318432808,
0.02655993029475212,
0.03258838877081871,
0.06772546470165253,
-0.0036186871584504843,
-0.022814976051449776,
-0.002172488486394286,
0.04964262619614601,
0.00044747701031155884,
-0.03017469123005867,
0.02323189564049244,
-0.01403801515698433,
0.004017603117972612,
0.04353382810950279,
-0.005791272036731243,
-0.051270004361867905,
0.04349224269390106,
0.037592414766550064,
-0.009686385281383991,
-0.05522098392248154,
0.13245047628879547,
0.026588594540953636,
-0.024109669029712677,
0.02065647765994072,
0.01981453225016594,
0.0704331248998642,
-0.029499750584363937,
-0.008299746550619602,
0.07438068836927414,
0.04476185515522957,
0.029347451403737068,
-0.01779961958527565,
0.011989395134150982,
-0.013533167541027069,
-0.007956079207360744,
0.006549254059791565,
0.009024222381412983,
0.03219706937670708,
0.014524566940963268,
-0.05312401428818703,
-0.02338012121617794,
-0.09983951598405838,
0.020304642617702484,
-0.01873941160738468,
0.0027160567697137594,
-0.038436390459537506,
0.014516078867018223,
-0.040394049137830734,
-0.014923612587153912,
-0.02844993956387043,
0.05813392251729965,
0.03429005667567253,
0.014760494232177734,
0.039135679602622986,
-0.016848092898726463,
-0.029851580038666725,
0.021886516362428665,
-0.01631474867463112,
0.016392404213547707,
-0.004583762958645821,
0.00486175250262022,
0.03712977096438408,
0.020542064681649208
] |
PMID:8284 | Lipid binding of a halothane metabolite. Relationship to lipid peroxidation in vitro. | Incubation of rat liver microsomes with 14C-halothane in a nitrogen atmosphere results in a high correlation between the formation of conjugated dienes and the binding of a halothane metabolite to phospholipids. Both the binding and the formation of the conjugated dienes required NADPH, were inhibited by carbon monoxide, and increased with duration of incubation and with protein concentration. Both [36Cl] halothane and [14C] halothane showed a similar pattern of binding to microsomal phospholipids suggesting that the chlorine atom is retained by the metabolite that binds. Neither high levels of conjugated dienes produced by halothane in microsomes incubated under nitrogen nor the binding of the halothane metabolite results in the destruction of cytochrome P-450. | Lipid binding of a halothane metabolite. Relationship to lipid peroxidation in vitro. Incubation of rat liver microsomes with 14C-halothane in a nitrogen atmosphere results in a high correlation between the formation of conjugated dienes and the binding of a halothane metabolite to phospholipids. Both the binding and the formation of the conjugated dienes required NADPH, were inhibited by carbon monoxide, and increased with duration of incubation and with protein concentration. Both [36Cl] halothane and [14C] halothane showed a similar pattern of binding to microsomal phospholipids suggesting that the chlorine atom is retained by the metabolite that binds. Neither high levels of conjugated dienes produced by halothane in microsomes incubated under nitrogen nor the binding of the halothane metabolite results in the destruction of cytochrome P-450. | [
-0.01633623242378235,
0.023518074303865433,
-0.02304905094206333,
-0.001353970030322671,
-0.004593532998114824,
-0.004488413222134113,
0.03920168802142143,
0.030619509518146515,
-0.0453757680952549,
-0.04894014447927475,
-0.022939130663871765,
-0.04033426567912102,
-0.0025852492544800043,
-0.008178026415407658,
0.002453771186992526,
-0.027717148885130882,
0.0008280358160845935,
-0.01566637121140957,
-0.07268471270799637,
0.009408161975443363,
-0.0306234247982502,
0.03710874170064926,
0.012436220422387123,
-0.02671121619641781,
0.004894943907856941,
0.0709862932562828,
0.021414747461676598,
0.007930642925202847,
-0.08929281681776047,
-0.21824443340301514,
-0.038368046283721924,
0.008318842388689518,
-0.008647482842206955,
0.01186556275933981,
-0.03551721200346947,
0.0393940731883049,
0.04453743249177933,
-0.03153044357895851,
0.02477157488465309,
0.06659119576215744,
0.07881534099578857,
0.023802317678928375,
-0.004402266349643469,
-0.01898125559091568,
0.051121629774570465,
0.03129421919584274,
0.018159329891204834,
-0.031151877716183662,
0.09820397198200226,
-0.04445500671863556,
0.0117717944085598,
-0.04966795817017555,
-0.007334060035645962,
0.013765525072813034,
0.022840801626443863,
-0.02828497439622879,
0.006827876437455416,
0.025821024551987648,
-0.010783609002828598,
0.018409734591841698,
-0.027405019849538803,
0.0405980721116066,
-0.1397375911474228,
0.058224134147167206,
-0.010340888053178787,
-0.03191196545958519,
-0.004111203830689192,
-0.03683345764875412,
0.05548664554953575,
0.061707813292741776,
-0.03463287651538849,
-0.027693789452314377,
-0.0266673993319273,
-0.026195470243692398,
0.0011146743781864643,
-0.009784160181879997,
-0.00045714565203525126,
-0.01874028891324997,
0.017274614423513412,
-0.03621038794517517,
-0.0005385908880271018,
-0.01727244071662426,
-0.07132977247238159,
0.003182225627824664,
-0.06304383277893066,
-0.0008600446744821966,
0.00920065026730299,
-0.04504571855068207,
-0.02866576798260212,
0.006410415284335613,
-0.004635998513549566,
-0.09836306422948837,
0.015726570039987564,
-0.03517339751124382,
-0.08935653418302536,
0.04158139228820801,
0.07599792629480362,
0.03228980675339699,
-0.006824991665780544,
0.34447482228279114,
-0.01962336152791977,
0.03021962009370327,
-0.09854524582624435,
0.0052981204353272915,
-0.014656277373433113,
-0.0010956781916320324,
-0.03881260007619858,
-0.035577669739723206,
0.040772076696157455,
-0.006943936925381422,
0.038229938596487045,
-0.008006504736840725,
0.0178096741437912,
-0.04248353838920593,
-0.1117432489991188,
0.01609271764755249,
0.04563410207629204,
0.0036542601883411407,
-0.002300938591361046,
0.006531970109790564,
0.02290576696395874,
-0.026841700077056885,
0.028472207486629486,
0.032972171902656555,
0.0479874461889267,
0.008067743852734566,
0.0004290096403565258,
0.09350123256444931,
0.019672619178891182,
0.009837196208536625,
0.05937512218952179,
0.01289138663560152,
0.026921458542346954,
0.0053082178346812725,
0.00230667507275939,
0.03445044532418251,
0.021031515672802925,
-0.05390488728880882,
-0.006768061779439449,
-0.03741050884127617,
-0.0187513530254364,
-0.03187200799584389,
-0.07790053635835648,
-0.0776790902018547,
-0.060294099152088165,
0.07199867069721222,
-0.005418295040726662,
0.02068083919584751,
0.00857599824666977,
-0.015318288467824459,
0.00006695320917060599,
0.049316536635160446,
-0.03356776013970375,
0.06556340306997299,
-0.06160137802362442,
0.011911215260624886,
0.017470944672822952,
0.014603178016841412,
0.00917140394449234,
-0.00978764332830906,
-0.030224403366446495,
-0.0051711248233914375,
0.03114144876599312,
0.1216522827744484,
-0.009103801101446152,
0.02045128494501114,
0.02964695543050766,
0.0405622273683548,
0.053528204560279846,
0.06612242013216019,
0.014448481611907482,
-0.04087552800774574,
0.025762656703591347,
-0.0031271481420844793,
-0.004530273377895355,
0.018181515857577324,
0.051591090857982635,
0.08742643892765045,
0.024771438911557198,
-0.018521828576922417,
0.010923527181148529,
-0.032322630286216736,
-0.0007721217698417604,
0.012236521579325199,
0.02069830149412155,
-0.024091579020023346,
-0.019288072362542152,
-0.002682649064809084,
-0.026297448202967644,
0.058987390249967575,
-0.008942246437072754,
0.016327902674674988,
-0.06088586896657944,
0.026763316243886948,
-0.07689943164587021,
0.01930541731417179,
-0.06823880225419998,
-0.0063790385611355305,
-0.016173087060451508,
-0.009581530466675758,
0.09613596647977829,
-0.023182787001132965,
-0.03916236758232117,
0.04108878970146179,
0.020504625514149666,
0.05424434691667557,
0.00599263608455658,
0.054045312106609344,
0.08082681894302368,
-0.018837913870811462,
-0.07165732979774475,
-0.012087090872228146,
-0.0036338630598038435,
-0.04238966107368469,
-0.018097074702382088,
-0.009987222030758858,
-0.03261974826455116,
0.009396546520292759,
0.046325769275426865,
-0.03509373590350151,
0.05348922684788704,
-0.07014857977628708,
-0.043947383761405945,
-0.33375489711761475,
-0.04076622426509857,
0.015120036900043488,
-0.005311221349984407,
0.04112594947218895,
-0.004614257253706455,
0.02158239297568798,
0.03269749879837036,
-0.04402586445212364,
-0.08369365334510803,
0.025953928008675575,
0.03825497254729271,
-0.04150771722197533,
-0.01703537628054619,
-0.011235378682613373,
0.04387715458869934,
0.03395611792802811,
-0.02167033590376377,
0.034390825778245926,
0.041321977972984314,
-0.023706819862127304,
-0.0417189858853817,
-0.03374263644218445,
-0.021427182480692863,
0.10770296305418015,
-0.012837180867791176,
0.103159099817276,
-0.021357284858822823,
-0.023570088669657707,
0.003289941232651472,
0.05613864213228226,
0.03817954659461975,
-0.038746148347854614,
-0.09803123027086258,
-0.037860363721847534,
-0.044043026864528656,
-0.04691705480217934,
-0.023749135434627533,
-0.004667932167649269,
0.004542735405266285,
-0.04163970798254013,
-0.006425280123949051,
-0.008235911838710308,
-0.017804928123950958,
-0.029231706634163857,
-0.03957971930503845,
-0.09138309955596924,
0.00009308850712841377,
-0.0693027451634407,
-0.0021388335153460503,
0.08929730206727982,
0.06923709809780121,
-0.01829569600522518,
-0.05318396911025047,
0.05648365989327431,
0.0027623786590993404,
-0.01745535619556904,
-0.04263785108923912,
-0.05202804505825043,
0.022793054580688477,
-0.012594136409461498,
0.01645462028682232,
0.02987704798579216,
-0.05869636684656143,
0.04197857156395912,
0.03183957189321518,
0.032766349613666534,
-0.012783510610461235,
0.0357840396463871,
0.01743273437023163,
0.0067317369394004345,
0.1158527359366417,
-0.04629352316260338,
-0.031141968443989754,
0.0634453073143959,
0.011958166025578976,
-0.06073032692074776,
-0.04979205131530762,
-0.08013482391834259,
0.08618729561567307,
0.0014953891513869166,
-0.027692830190062523,
0.02262723818421364,
0.04596370831131935,
-0.01204785704612732,
-0.008386773988604546,
0.03901757299900055,
0.005616599693894386,
0.04661836847662926,
0.04558027535676956,
-0.00817049853503704,
0.010692575015127659,
-0.017461609095335007,
-0.07583074271678925,
0.023458370938897133,
0.05069584771990776,
-0.22496074438095093,
0.009160416200757027,
0.015586073510348797,
0.019838741049170494,
-0.012113777920603752,
-0.015393773093819618,
0.03427400812506676,
0.011159420013427734,
-0.05424603447318077,
-0.005728962831199169,
0.017068834975361824,
0.040021587163209915,
-0.005517639685422182,
0.061699509620666504,
0.021457448601722717,
0.016040541231632233,
0.017209334298968315,
-0.009188373573124409,
-0.030709158629179,
-0.01725790649652481,
-0.028731297701597214,
-0.061864934861660004,
0.14967676997184753,
0.012172623537480831,
-0.0055867694318294525,
0.046655040234327316,
0.026108063757419586,
0.08014538884162903,
-0.026481015607714653,
0.05909479036927223,
-0.059454064816236496,
-0.008540173061192036,
0.10278687626123428,
-0.014006728306412697,
0.013691826723515987,
0.059638768434524536,
-0.03005824238061905,
-0.0013939071213826537,
-0.0021379562094807625,
-0.020191939547657967,
0.010849256068468094,
0.02297576516866684,
0.003956716042011976,
-0.05386187881231308,
0.10055240988731384,
-0.05547301098704338,
0.009509864263236523,
-0.03865137696266174,
0.03598795831203461,
-0.023868262767791748,
0.00026549454196356237,
0.03505665063858032,
-0.023055901750922203,
0.06154000014066696,
0.031022919341921806,
-0.02043013833463192,
-0.024516530334949493,
-0.00904494896531105,
0.012241609394550323,
0.07106313854455948,
-0.022125812247395515,
0.0068195476196706295,
-0.0018341040704399347,
0.06341122835874557,
0.10852476209402084
] |
PMID:8285 | Studies on the mechanism of toxicity and of development of tolerance to the pulmonary toxin, alpha-naphthylthiourea (ANTU). | The in vivo administration of the radiolabeled lung toxin alpha-naphthylthiourea (ANTU) to rats leads to the covalent binding of radioactivity to the macromolecules of the lung and liver. In contrast, very little radioactivity is bound in these organs when an equal amount of the 14C-labeled oxygen analog of ANTU, 14C-alpha-naphthylurea (ANU), is administered. In addition, ANU is essentially nontoxic to rats. ANTU is metabolized in vitro by lung and liver microsomes to an intermediate which covalently binds to the macromolecules of the microsomes. This covalent binding, which requires NADPH, leads to a decrease in mixed-function oxidase activity and to a decrease in the level of cytochrome P-450 detectable as its carbon monoxide complex. Incubation of microsomes with ANTU in the absence of NADPH or with ANU in the presence of NADPH, has no effect on these parameters. Pretreatment of rats with small nonlethal doses of ANTU daily for 5 days brings about a decrease in the activity of the mixed-function oxidase enzyme system in the lung which metabolizes parathion. In addition, this pretreatment decreases the toxicity of ANTU and leads to a decrease in the amount of radioactivity bound to the macromolecules of the lung when the animals are given a lethal dose of 35S-ANTU. These data suggest that the lung toxicity of ANTU is brought about by its metabolic activation and covalent binding to lung macromolecules. | Studies on the mechanism of toxicity and of development of tolerance to the pulmonary toxin, alpha-naphthylthiourea (ANTU). The in vivo administration of the radiolabeled lung toxin alpha-naphthylthiourea (ANTU) to rats leads to the covalent binding of radioactivity to the macromolecules of the lung and liver. In contrast, very little radioactivity is bound in these organs when an equal amount of the 14C-labeled oxygen analog of ANTU, 14C-alpha-naphthylurea (ANU), is administered. In addition, ANU is essentially nontoxic to rats. ANTU is metabolized in vitro by lung and liver microsomes to an intermediate which covalently binds to the macromolecules of the microsomes. This covalent binding, which requires NADPH, leads to a decrease in mixed-function oxidase activity and to a decrease in the level of cytochrome P-450 detectable as its carbon monoxide complex. Incubation of microsomes with ANTU in the absence of NADPH or with ANU in the presence of NADPH, has no effect on these parameters. Pretreatment of rats with small nonlethal doses of ANTU daily for 5 days brings about a decrease in the activity of the mixed-function oxidase enzyme system in the lung which metabolizes parathion. In addition, this pretreatment decreases the toxicity of ANTU and leads to a decrease in the amount of radioactivity bound to the macromolecules of the lung when the animals are given a lethal dose of 35S-ANTU. These data suggest that the lung toxicity of ANTU is brought about by its metabolic activation and covalent binding to lung macromolecules. | [
-0.06184231489896774,
0.0318625383079052,
-0.03991692513227463,
0.002389986300840974,
0.03072858601808548,
0.019294217228889465,
0.07119783759117126,
0.008393102325499058,
-0.07169084995985031,
0.022729074582457542,
-0.00771671999245882,
-0.09938626736402512,
-0.03725564852356911,
0.054269544780254364,
-0.008527028374373913,
-0.03213195875287056,
-0.01269198302179575,
-0.011378188617527485,
-0.04686606302857399,
0.042754318565130234,
0.002460248302668333,
0.010439892299473286,
0.04525187611579895,
-0.020300904288887978,
0.03883115574717522,
0.09388454258441925,
0.005158924497663975,
-0.03940404951572418,
-0.04027850925922394,
-0.2065262794494629,
-0.012016983702778816,
0.02426239848136902,
-0.013764922507107258,
-0.04009639844298363,
-0.04040846601128578,
0.022632775828242302,
0.037583593279123306,
-0.016658322885632515,
-0.0012804742436856031,
0.07658912986516953,
0.008616462349891663,
0.054808225482702255,
0.030598046258091927,
-0.01635441742837429,
-0.034246936440467834,
-0.004827465862035751,
-0.061590153723955154,
-0.03005329519510269,
0.09906623512506485,
0.014344275929033756,
0.031476639211177826,
0.020976057276129723,
0.028501713648438454,
0.027088142931461334,
-0.005297041032463312,
-0.044439349323511124,
0.05166123807430267,
0.08665721863508224,
-0.006664634682238102,
0.058167338371276855,
-0.03498189151287079,
0.014993836171925068,
-0.13078774511814117,
0.04917019605636597,
0.06053239479660988,
-0.0005614769761450589,
-0.008374880068004131,
-0.056296274065971375,
0.030161088332533836,
0.026660064235329628,
-0.06061103940010071,
0.05614138022065163,
0.01877632923424244,
0.004751621279865503,
0.016653168946504593,
0.0016150675946846604,
0.025657856836915016,
-0.028651373460888863,
0.057078517973423004,
0.02470986917614937,
-0.051264453679323196,
-0.014873656444251537,
0.011707061901688576,
-0.013335153460502625,
-0.0009217262268066406,
-0.020211493596434593,
-0.011596713215112686,
-0.05004515126347542,
-0.030750878155231476,
0.03897066041827202,
-0.010039056651294231,
-0.03250303864479065,
0.005709639750421047,
0.01972777396440506,
-0.022562898695468903,
-0.030531669035553932,
0.0014965430600568652,
0.008079282008111477,
-0.037333790212869644,
0.33626750111579895,
-0.010271169245243073,
0.029852621257305145,
-0.06347920745611191,
-0.015751004219055176,
-0.02038494125008583,
-0.013887684792280197,
-0.046496618539094925,
-0.02123946323990822,
0.03947586193680763,
0.04776819422841072,
0.04354175552725792,
0.04244941845536232,
-0.0018172092968598008,
-0.006563606671988964,
-0.006786069832742214,
0.03424961492419243,
0.036181043833494186,
0.006104680243879557,
-0.017206160351634026,
0.00017159292474389076,
-0.03002031333744526,
-0.06426867097616196,
-0.03468213975429535,
0.012292766012251377,
0.0600985623896122,
-0.02626386284828186,
-0.002749484498053789,
0.062346480786800385,
0.051150042563676834,
0.07287833839654922,
0.06761399656534195,
-0.04172328859567642,
0.02880694903433323,
0.017815250903367996,
-0.003679734654724598,
-0.004286819603294134,
-0.04067780449986458,
-0.06903640925884247,
-0.026008760556578636,
0.008644161745905876,
-0.05858095362782478,
-0.04598655551671982,
-0.02133818343281746,
-0.10440289229154587,
-0.0629296526312828,
0.10254873335361481,
0.040173277258872986,
0.014748531393706799,
-0.006975023541599512,
-0.00008446457650279626,
0.014291122555732727,
0.0464339554309845,
-0.0180018562823534,
0.041839856654405594,
-0.07654472440481186,
0.013604164123535156,
-0.02520461566746235,
0.02771603688597679,
0.039033446460962296,
-0.023347564041614532,
-0.06303194165229797,
-0.04219602793455124,
0.0074297054670751095,
0.09054114669561386,
-0.02001205086708069,
0.016694001853466034,
-0.024868082255125046,
0.020987704396247864,
0.013663530349731445,
0.027578093111515045,
0.017739243805408478,
0.017224838957190514,
0.01429263036698103,
-0.05526476725935936,
-0.03737156093120575,
0.011000357568264008,
0.007462978828698397,
-0.011314497329294682,
0.012482073158025742,
0.0013381410390138626,
0.03433772176504135,
-0.03984244167804718,
-0.012383531779050827,
0.007990998215973377,
0.04448252171278,
0.02351764403283596,
-0.033063407987356186,
0.044951993972063065,
0.03163310885429382,
-0.014637915417551994,
-0.034241728484630585,
0.04304567351937294,
-0.0950586274266243,
-0.01650375872850418,
0.001871143002063036,
-0.06390608847141266,
-0.030062060803174973,
-0.021658116951584816,
-0.020132359117269516,
0.019894367083907127,
0.12691085040569305,
0.026106305420398712,
0.024528011679649353,
0.0088724410161376,
0.01429635751992464,
0.002185297664254904,
0.02805713191628456,
0.02780262753367424,
0.02875964716076851,
-0.045319076627492905,
-0.07041994482278824,
-0.00511366268619895,
-0.043748196214437485,
0.029125483706593513,
-0.06433437019586563,
0.08131895214319229,
0.022100139409303665,
-0.045317549258470535,
0.058772481977939606,
0.052646249532699585,
0.028200507164001465,
-0.03294586017727852,
-0.01617763563990593,
-0.35053980350494385,
-0.060777951031923294,
0.031759120523929596,
-0.032760605216026306,
0.05717489495873451,
-0.007166732102632523,
-0.014263642020523548,
0.00398235721513629,
0.022158846259117126,
-0.047182366251945496,
0.05772245675325394,
0.06749706715345383,
-0.031823646277189255,
-0.019097650423645973,
-0.008578001521527767,
0.05372444540262222,
-0.016196338459849358,
-0.02740018628537655,
0.00007829908281564713,
0.007720134221017361,
-0.057515934109687805,
-0.03956889361143112,
-0.0023811585269868374,
-0.006174181122332811,
0.03384259343147278,
-0.045862458646297455,
0.11522172391414642,
-0.013997508212924004,
-0.08230223506689072,
0.014003923162817955,
0.012223739176988602,
0.03179623559117317,
0.0009925311896950006,
-0.0739680752158165,
-0.04085517302155495,
-0.01622491516172886,
-0.011308948509395123,
-0.030788401141762733,
-0.02549717389047146,
0.03182963281869888,
-0.03613652288913727,
0.004462269600480795,
-0.0263564120978117,
-0.09135393798351288,
-0.08108409494161606,
-0.019272103905677795,
0.024292226880788803,
-0.034357380121946335,
-0.03851372003555298,
0.00037910998798906803,
0.040596410632133484,
0.026003381237387657,
-0.0030517075210809708,
0.0046603200025856495,
0.005053952801972628,
-0.009571227245032787,
0.007532333955168724,
-0.007900669239461422,
-0.01853424496948719,
0.005072231870144606,
0.00020846047846134752,
0.018640771508216858,
0.008773740381002426,
-0.04108667001128197,
0.014935381710529327,
-0.05361691862344742,
0.03561442717909813,
-0.03984355553984642,
0.020913703367114067,
0.038299981504678726,
-0.02480253390967846,
0.10964611172676086,
-0.014742757193744183,
0.037434566766023636,
0.01598191447556019,
0.023562943562865257,
-0.0379098579287529,
-0.034212835133075714,
-0.03436414152383804,
0.04850362613797188,
0.032361991703510284,
-0.009167343378067017,
0.12299294024705887,
0.02730574831366539,
-0.09445372223854065,
0.059842582792043686,
0.03963056951761246,
-0.028544578701257706,
0.05169646441936493,
0.00944732315838337,
0.030833126977086067,
0.016308266669511795,
-0.009143505245447159,
-0.031079884618520737,
0.05737796053290367,
0.07095927745103836,
-0.28005582094192505,
0.017423326149582863,
0.0012236982583999634,
0.03732070326805115,
-0.02026917226612568,
-0.020105503499507904,
0.034642014652490616,
0.03421695530414581,
-0.014987785369157791,
0.003920226823538542,
0.025974968448281288,
0.03605359420180321,
0.04652830958366394,
0.0046192980371415615,
-0.015312297269701958,
0.030589578673243523,
0.02086012251675129,
0.010949980467557907,
-0.03367011249065399,
0.025491544976830482,
-0.028941286727786064,
-0.054304711520671844,
0.10410725325345993,
-0.0005636708810925484,
-0.0321115143597126,
0.06647048890590668,
-0.05727335065603256,
0.04178275540471077,
-0.043845631182193756,
0.026088569313287735,
0.0330696739256382,
-0.028669582679867744,
0.0635591670870781,
-0.08715005218982697,
0.048075344413518906,
0.006642325781285763,
-0.04177438095211983,
0.027759751304984093,
-0.006619034800678492,
-0.004346828442066908,
-0.05325712263584137,
0.021005477756261826,
-0.011059888638556004,
-0.04379603639245033,
0.09116888791322708,
-0.004600833170115948,
-0.05925319343805313,
0.0467928983271122,
-0.01144756656140089,
0.026110917329788208,
-0.04082215577363968,
-0.02377398870885372,
0.048574525862932205,
0.04887702316045761,
0.03824448585510254,
-0.042420826852321625,
-0.023556647822260857,
0.026557065546512604,
-0.04385005310177803,
-0.023520469665527344,
0.031076589599251747,
0.05193031579256058,
-0.057685960084199905,
0.08440709114074707,
0.06307470798492432
] |
PMID:8286 | Stereospecific binding of timolol, a beta-adrenergic blocking agent. | The beta-adrenergic blocking agent, timolol, appears to be bound to stereospecific as well as nonspecific sites in the particulate fraction (8500g pellet) of the heart, lungs, and brain, whereas the d-isomer of timolol was bound to nonspecific sites only. Timolol disappeared from the particulate fraction at a slower rate than did its optical isomer. At 1 hr after a 0.1-mg/kg dose, the concentration of the l-form in the lung was 1.8 times that of the d-isomer and at 3 and 4 hr the difference was at least 33-fold. The concentration of 14C-timolol in the particulate fraction of rat tissues was inhibited by iv administered timolol and by the l-isomer of propanolol, but not by their corresponding d-forms. Competition for binding sites was dose dependent. Pretreatment with timolol at 0.1 and 5.0 mg/kg reduced the binding of 14C-timolol (dose, 0.1 mg/kg) to lung tissue by 41% and 86%, respectively. In the heart and lung tissue of rats, racemic timolol, propranolol, bunolol, and bunitrolol were approximately equally effective in competing for the binding sites of 14C-timolol. Practolol and sotalol and the beta 2-selective agent butoxamine did not significantly inhibit the binding of 14C-timolol. Similar competition was also observed in the whole brain of rats. This report suggests that the stereospecific binding of timolol may be related to the beta-adrenoreceptor process. | Stereospecific binding of timolol, a beta-adrenergic blocking agent. The beta-adrenergic blocking agent, timolol, appears to be bound to stereospecific as well as nonspecific sites in the particulate fraction (8500g pellet) of the heart, lungs, and brain, whereas the d-isomer of timolol was bound to nonspecific sites only. Timolol disappeared from the particulate fraction at a slower rate than did its optical isomer. At 1 hr after a 0.1-mg/kg dose, the concentration of the l-form in the lung was 1.8 times that of the d-isomer and at 3 and 4 hr the difference was at least 33-fold. The concentration of 14C-timolol in the particulate fraction of rat tissues was inhibited by iv administered timolol and by the l-isomer of propanolol, but not by their corresponding d-forms. Competition for binding sites was dose dependent. Pretreatment with timolol at 0.1 and 5.0 mg/kg reduced the binding of 14C-timolol (dose, 0.1 mg/kg) to lung tissue by 41% and 86%, respectively. In the heart and lung tissue of rats, racemic timolol, propranolol, bunolol, and bunitrolol were approximately equally effective in competing for the binding sites of 14C-timolol. Practolol and sotalol and the beta 2-selective agent butoxamine did not significantly inhibit the binding of 14C-timolol. Similar competition was also observed in the whole brain of rats. This report suggests that the stereospecific binding of timolol may be related to the beta-adrenoreceptor process. | [
-0.048639461398124695,
-0.0017393928719684482,
-0.007399430498480797,
-0.02024657279253006,
0.06109187752008438,
0.008956057019531727,
0.06739109754562378,
0.02717376872897148,
-0.05964900180697441,
-0.007091233506798744,
0.04194432497024536,
-0.03485224395990372,
0.01082178857177496,
0.03399262949824333,
-0.004035162273794413,
0.023052196949720383,
0.03359140828251839,
-0.028371648862957954,
-0.04170065373182297,
-0.01230714563280344,
0.007663119118660688,
-0.016688531264662743,
0.03779394179582596,
0.007568365894258022,
0.05288213863968849,
0.013728661462664604,
-0.023959705606102943,
-0.04848931357264519,
0.005062741227447987,
-0.2007085680961609,
0.04469335451722145,
-0.0004588040173985064,
-0.008133349940180779,
0.007635389920324087,
-0.018584758043289185,
0.014239559881389141,
-0.030540088191628456,
0.00032495451159775257,
-0.008653370663523674,
0.020461127161979675,
0.030726268887519836,
0.019075248390436172,
-0.0006148825632408261,
0.002489158883690834,
0.027227571234107018,
0.04601847380399704,
-0.07000607252120972,
-0.005301908124238253,
0.06068391352891922,
-0.0075171939097344875,
0.02168276719748974,
0.004347584675997496,
0.005786261521279812,
0.05306297913193703,
-0.04073060676455498,
-0.0006446587503887713,
0.06673706322908401,
0.037848297506570816,
-0.015366465784609318,
0.05662684887647629,
-0.030951591208577156,
0.057509779930114746,
-0.12440613657236099,
0.07300713658332825,
0.0319163016974926,
0.016650445759296417,
-0.033174462616443634,
-0.007014045491814613,
0.04096574708819389,
0.09456530213356018,
0.014686845242977142,
0.07105446606874466,
0.021192600950598717,
-0.020630935207009315,
0.06155947223305702,
0.000684030877891928,
-0.013043588027358055,
-0.0028931263368576765,
0.019558638334274292,
-0.04709859564900398,
0.013031842187047005,
0.041886843740940094,
-0.04636654257774353,
-0.046206600964069366,
-0.02895977348089218,
-0.0020584051962941885,
0.07283985614776611,
-0.03623143583536148,
-0.02639484778046608,
-0.005796391051262617,
-0.008590797893702984,
-0.057570695877075195,
0.0018999974709004164,
-0.034169040620326996,
-0.08177822083234787,
0.010513568297028542,
-0.015630381181836128,
0.00648358091711998,
0.0013031534617766738,
0.3587047755718231,
0.010723751969635487,
0.057295385748147964,
-0.05275453254580498,
0.0415046326816082,
0.05773487687110901,
-0.024121515452861786,
-0.0023108557797968388,
-0.07288488745689392,
-0.03289606794714928,
0.03329616039991379,
0.042942218482494354,
-0.0357905849814415,
0.03589039668440819,
-0.009036088362336159,
-0.028134800493717194,
-0.049220893532037735,
0.01341406349092722,
-0.052923548966646194,
0.0028756267856806517,
-0.03399992734193802,
-0.08192212134599686,
0.01869090087711811,
0.024341212585568428,
0.020154275000095367,
0.04197010397911072,
0.002056659199297428,
-0.024971595034003258,
0.0975290909409523,
0.06205516681075096,
0.017481928691267967,
0.05651039630174637,
0.03078986331820488,
-0.003934159409254789,
0.043645162135362625,
0.06549675762653351,
-0.06198281794786453,
0.044328100979328156,
-0.028514130041003227,
0.016451386734843254,
-0.013988655991852283,
-0.03979162499308586,
-0.048633333295583725,
0.006400969810783863,
-0.046462055295705795,
-0.03561006858944893,
0.10942807793617249,
0.0016048973193392158,
-0.003018004819750786,
-0.045577265322208405,
0.0047233616933226585,
-0.04414929077029228,
0.0552804060280323,
-0.007639401126652956,
0.051843173801898956,
-0.06408947706222534,
-0.002801246475428343,
-0.0007567760185338557,
0.026241939514875412,
-0.03975502401590347,
0.000624355161562562,
-0.05283903330564499,
-0.0391891673207283,
-0.002315464662387967,
0.09858745336532593,
-0.028508875519037247,
0.006356486119329929,
-0.04538262262940407,
0.015242521651089191,
0.01866977848112583,
-0.014102241024374962,
-0.0155025664716959,
-0.03939604386687279,
0.011151817627251148,
-0.04901434853672981,
-0.08824562281370163,
0.03331773355603218,
-0.012943880632519722,
0.006254539359360933,
0.04225606471300125,
-0.0651850774884224,
0.05054304376244545,
0.0055684312246739864,
-0.029642025008797646,
0.0027695728931576014,
0.011831765063107014,
0.027047544717788696,
-0.0029625645838677883,
-0.06609644740819931,
-0.026265880092978477,
0.06706549227237701,
0.03329393267631531,
0.00651014968752861,
-0.06603703647851944,
-0.003370879217982292,
-0.020565513521432877,
-0.03930198773741722,
-0.02416365034878254,
0.023443227633833885,
-0.0014246383216232061,
0.0032180652488023043,
0.10464610904455185,
-0.008687343448400497,
-0.020132357254624367,
0.025643883273005486,
0.049900565296411514,
0.07754124701023102,
0.0016378987347707152,
-0.03646976873278618,
0.10038118064403534,
-0.006796041037887335,
0.0007922245422378182,
0.02452711947262287,
-0.010314978659152985,
0.012888445518910885,
-0.007457623723894358,
0.044201258569955826,
-0.0003588457766454667,
-0.034224532544612885,
0.04521379619836807,
0.058936309069395065,
0.0022672577761113644,
-0.013973063789308071,
-0.00964201521128416,
-0.2995412349700928,
-0.022467250004410744,
-0.028200039640069008,
0.007190308533608913,
0.04951149970293045,
-0.052302174270153046,
-0.012479344382882118,
-0.01966407150030136,
-0.00664706202223897,
-0.04236792400479317,
0.03738156706094742,
0.05485275387763977,
-0.04233067110180855,
-0.03130381181836128,
-0.04959636554121971,
0.030964769423007965,
0.038369014859199524,
-0.01766037940979004,
-0.015291554853320122,
-0.026489516720175743,
-0.004324275068938732,
-0.07023985683917999,
-0.07618030160665512,
0.023544931784272194,
0.07667651772499084,
-0.04588279128074646,
0.1134340763092041,
0.009314876981079578,
-0.024150004610419273,
0.012191654182970524,
-0.03712547942996025,
0.016329757869243622,
-0.029414182528853416,
-0.10065094381570816,
-0.0030112294480204582,
-0.04631347209215164,
0.025346023961901665,
-0.040594421327114105,
0.02147766575217247,
0.0026977993547916412,
-0.05287919566035271,
0.0330640934407711,
0.0033104000613093376,
-0.038587525486946106,
-0.07432977110147476,
-0.009319532662630081,
0.006921624764800072,
0.09904508292675018,
-0.01918957009911537,
-0.011772344820201397,
0.020882077515125275,
-0.005234622862190008,
-0.004629118368029594,
-0.04014153406023979,
0.01153478305786848,
0.008932318538427353,
-0.040580909699201584,
-0.04565250128507614,
-0.009728820994496346,
-0.012595337815582752,
0.025571392849087715,
0.004779286682605743,
0.05498690530657768,
0.010138348676264286,
-0.03376685455441475,
0.035953495651483536,
0.03089824691414833,
-0.06968686729669571,
0.05818754807114601,
0.044892460107803345,
0.02342611737549305,
0.11040794849395752,
-0.05207240581512451,
0.050857387483119965,
0.058125000447034836,
0.024864280596375465,
-0.025922799482941628,
-0.01843760907649994,
-0.0395088829100132,
0.0037892726249992847,
0.028613096103072166,
-0.04133250191807747,
0.027054505422711372,
-0.008565515279769897,
-0.03115018829703331,
-0.03669706732034683,
0.02460276335477829,
-0.02516794763505459,
0.05509716272354126,
0.008649162948131561,
-0.022696267813444138,
0.017419524490833282,
-0.022401273250579834,
-0.06114732474088669,
0.05820433050394058,
-0.0023362888023257256,
-0.27832213044166565,
0.06081938371062279,
0.05510765686631203,
0.05267806351184845,
-0.011728157289326191,
-0.04041757062077522,
0.040119558572769165,
-0.009936182759702206,
-0.049518436193466187,
-0.008459891192615032,
-0.03927110135555267,
0.02760370261967182,
0.04248281940817833,
0.05548122152686119,
-0.05955246835947037,
-0.011743112467229366,
0.06626498699188232,
-0.041408147662878036,
0.027610348537564278,
0.0005268107634037733,
-0.044335849583148956,
-0.02127380296587944,
0.17436163127422333,
0.034676358103752136,
-0.04496552422642708,
0.08592211455106735,
-0.03046359308063984,
-0.00039000503602437675,
-0.06041039898991585,
0.04951053857803345,
-0.012881333008408546,
-0.014903933741152287,
0.06572072952985764,
-0.07196063548326492,
-0.0025645603891462088,
0.039805445820093155,
-0.06332583725452423,
0.00741211324930191,
0.03402681276202202,
0.026443975046277046,
-0.06361418962478638,
-0.0005417575594037771,
-0.06602008640766144,
-0.021209772676229477,
0.07452146708965302,
-0.10746203362941742,
-0.03009868785738945,
0.012677551247179508,
0.01724941097199917,
0.009884319268167019,
-0.016299152746796608,
0.029684972018003464,
-0.05253855139017105,
0.0668201893568039,
0.011715460568666458,
0.00859533529728651,
0.005685919895768166,
-0.0019607029389590025,
-0.013431847095489502,
0.004999061580747366,
-0.04067718982696533,
-0.03287883475422859,
-0.0467621311545372,
0.054902978241443634,
0.08668050169944763
] |
PMID:8287 | Inversion of optical configuration of alpha-methylfluorene-2-acetic acid (cicloprofen) in rats and monkeys. | A simple and sensitive radiometric method to determine the individual enantiomers of cicloprofen has been developed. 14C-Cicloprofen was converted to its L-leucine diastereoisomers, which were separated by thin-layer chromatography and quantified by measuring the radioactivity in the area corresponding to each individual diastereoisomer. This technique has also been used to measure the enantiomers of unlabeled cicloprofen by condensing with 14C-labeled L-leucine. By using the radiometric method, a unique biotransformation process, the inversion of the (-)-enantiomer of alpha-methylfluorene-2-acetic acid to its (+)-enantiomer, has been demonstrated in the rat and monkey. The rate of (-)- to (+)-inversion was found to be faster in the rat than in the monkey. After single or repeated oral adminstration of the racemic modification or the (-)-enantiomer of cicloprofen to both species, the ratio of (+)- to (-)-enantiomers of cicloprofen in plasma, urine, or bile increased with time. At 5, 22, and 48 hr after oral administration of a single 50-mg/kg dose of the (-)-enantiomer, 14C-cicloprofen in rat plasma contained 20, 50, and 79%, respectively, of the (+)-enantiomer. After receiving the same dose of (-)-enantiomer, monkey plasma contained 16.5% and 32% of (+)-enantiomer at 8 and 24 hr, respectively. After oral administration of a single 50-mg/kg dose of the (+)-enantiomer of 14C-cicloprofen to rats and monkeys, the percentage of (-)-enantiomer in plasma varied from 2 to 15%. Since the administered (+)-enantiomer contained 4% of (-)-enantiomer and the (+)-enantiomer was excreted at a faster rate than its (-)-antipode by rats or monkeys, it is not known whether an occasional small percentage increase of (-)-enantiomer in plasma resulted from the (+)-to-(-) inversion, or from faster elimination of the (+)-enantiomer. Nevertheless, if (+)-to-(-) inversion does occur in these two species, the rate is much slower than for the (-)-to-(+) inversion. | Inversion of optical configuration of alpha-methylfluorene-2-acetic acid (cicloprofen) in rats and monkeys. A simple and sensitive radiometric method to determine the individual enantiomers of cicloprofen has been developed. 14C-Cicloprofen was converted to its L-leucine diastereoisomers, which were separated by thin-layer chromatography and quantified by measuring the radioactivity in the area corresponding to each individual diastereoisomer. This technique has also been used to measure the enantiomers of unlabeled cicloprofen by condensing with 14C-labeled L-leucine. By using the radiometric method, a unique biotransformation process, the inversion of the (-)-enantiomer of alpha-methylfluorene-2-acetic acid to its (+)-enantiomer, has been demonstrated in the rat and monkey. The rate of (-)- to (+)-inversion was found to be faster in the rat than in the monkey. After single or repeated oral adminstration of the racemic modification or the (-)-enantiomer of cicloprofen to both species, the ratio of (+)- to (-)-enantiomers of cicloprofen in plasma, urine, or bile increased with time. At 5, 22, and 48 hr after oral administration of a single 50-mg/kg dose of the (-)-enantiomer, 14C-cicloprofen in rat plasma contained 20, 50, and 79%, respectively, of the (+)-enantiomer. After receiving the same dose of (-)-enantiomer, monkey plasma contained 16.5% and 32% of (+)-enantiomer at 8 and 24 hr, respectively. After oral administration of a single 50-mg/kg dose of the (+)-enantiomer of 14C-cicloprofen to rats and monkeys, the percentage of (-)-enantiomer in plasma varied from 2 to 15%. Since the administered (+)-enantiomer contained 4% of (-)-enantiomer and the (+)-enantiomer was excreted at a faster rate than its (-)-antipode by rats or monkeys, it is not known whether an occasional small percentage increase of (-)-enantiomer in plasma resulted from the (+)-to-(-) inversion, or from faster elimination of the (+)-enantiomer. Nevertheless, if (+)-to-(-) inversion does occur in these two species, the rate is much slower than for the (-)-to-(+) inversion. | [
-0.045730069279670715,
-0.048037946224212646,
-0.03127322345972061,
0.012898437678813934,
0.06501854956150055,
-0.01787731610238552,
0.04479286074638367,
0.025940541177988052,
-0.06083643436431885,
0.02239939384162426,
0.04008115828037262,
-0.05411889776587486,
-0.003939551301300526,
-0.03016498312354088,
-0.03449079394340515,
-0.013447215780615807,
-0.024767810478806496,
-0.02318575605750084,
-0.003136155428364873,
-0.04522383585572243,
-0.012074321508407593,
-0.055426206439733505,
0.08262299746274948,
-0.023585299029946327,
0.0028846045024693012,
0.07764981687068939,
-0.0244407057762146,
-0.02038636989891529,
-0.054892491549253464,
-0.20706115663051605,
0.054098837077617645,
-0.0041167545132339,
0.012773392722010612,
-0.028543483465909958,
-0.001760402461513877,
-0.04247203469276428,
-0.050566356629133224,
0.00555375823751092,
-0.010526937432587147,
0.029097026214003563,
0.08694244921207428,
-0.0006544806528836489,
-0.04169807583093643,
0.06899839639663696,
0.005061013624072075,
-0.008229942992329597,
-0.0875047966837883,
0.03193153068423271,
0.09279992431402206,
0.014221937395632267,
0.06599103659391403,
-0.0007934970781207085,
0.012234346941113472,
0.05210643261671066,
-0.03020571544766426,
-0.02256961725652218,
0.03812823444604874,
0.0416986308991909,
-0.004901663400232792,
0.06878888607025146,
0.01577727496623993,
0.044927675276994705,
-0.13991418480873108,
0.06919091939926147,
0.001166676520369947,
-0.014092549681663513,
-0.02611006237566471,
-0.07396703958511353,
-0.0205087810754776,
0.10847344994544983,
-0.05425513908267021,
0.06357646733522415,
0.010589689947664738,
-0.043948784470558167,
0.06179508939385414,
-0.010096805170178413,
0.014635201543569565,
-0.04891155660152435,
0.018873421475291252,
-0.005909198895096779,
0.05640596151351929,
0.09551575034856796,
-0.004098745062947273,
-0.05800624564290047,
0.06338546425104141,
-0.013483384624123573,
0.035646937787532806,
-0.01275398675352335,
0.04308716207742691,
0.0427665039896965,
-0.03754259645938873,
-0.044587939977645874,
-0.013286419212818146,
-0.014042487367987633,
-0.03625846281647682,
-0.012137938290834427,
0.022372914478182793,
0.011423200368881226,
0.002782572992146015,
0.36102205514907837,
0.012417232617735863,
0.017127221450209618,
-0.08683934807777405,
0.0028855600394308567,
-0.012654845602810383,
-0.049384914338588715,
0.019271768629550934,
-0.0020672876853495836,
0.019325358793139458,
0.03617605194449425,
0.030425503849983215,
0.02868184819817543,
0.06730949133634567,
-0.02412925288081169,
-0.06164544075727463,
-0.03397731855511665,
0.06289514154195786,
0.04008994996547699,
0.0022577412892132998,
0.0037935026921331882,
0.0005900983815081418,
0.009414727799594402,
0.0404333658516407,
-0.011384916491806507,
0.04410536587238312,
-0.010822467505931854,
0.006279542576521635,
0.06423430144786835,
0.05428140610456467,
0.03140689805150032,
0.04621679708361626,
-0.022283079102635384,
0.001753602409735322,
0.006098735146224499,
0.021526120603084564,
-0.0032348670065402985,
0.027724480256438255,
0.020466851070523262,
0.009422548115253448,
0.06243176385760307,
0.0007939362549223006,
-0.0834803506731987,
-0.026524465531110764,
-0.049550171941518784,
-0.04303574562072754,
0.16637827455997467,
0.02640390396118164,
-0.001402271562255919,
-0.04338156431913376,
-0.02344454638659954,
0.0002812191378325224,
0.041372984647750854,
-0.00035563696292228997,
0.0169102493673563,
0.024207379668951035,
0.03264296054840088,
0.020601928234100342,
-0.023288758471608162,
-0.05986405909061432,
0.006176634691655636,
-0.04055657982826233,
-0.04343277961015701,
-0.03205067291855812,
0.08508304506540298,
-0.015611164271831512,
-0.02788768708705902,
-0.028773490339517593,
0.028682531788945198,
0.034015052020549774,
0.026113074272871017,
-0.039206769317388535,
-0.013716018758714199,
-0.04410916566848755,
0.01223340816795826,
-0.02338777296245098,
0.009776216000318527,
0.0001290661166422069,
-0.01341339759528637,
-0.02413897216320038,
-0.016674740239977837,
0.041370801627635956,
-0.007958726957440376,
-0.034823011606931686,
0.06422199308872223,
-0.019721297547221184,
0.016664881259202957,
-0.05520840361714363,
-0.0004876717575825751,
-0.039453163743019104,
0.008400638587772846,
-0.018590029329061508,
0.029313893988728523,
-0.054765257984399796,
-0.00689220754429698,
-0.07202758640050888,
-0.011727039702236652,
-0.04216686263680458,
0.015104930847883224,
-0.0027334459591656923,
-0.02645515836775303,
0.09128091484308243,
0.009693421423435211,
0.03955088555812836,
0.016365649178624153,
0.03659501671791077,
0.017775755375623703,
-0.0035863302182406187,
-0.019342884421348572,
0.054144520312547684,
-0.015672262758016586,
-0.008794025518000126,
0.026827944442629814,
0.0042787278071045876,
0.013784995302557945,
-0.031865429133176804,
0.04733194783329964,
0.005543834529817104,
-0.018250711262226105,
0.03219706937670708,
0.037394892424345016,
-0.048059672117233276,
-0.04101810231804848,
-0.03332884982228279,
-0.2916629910469055,
-0.03252200037240982,
-0.0010338308056816459,
-0.028113801032304764,
0.06331434845924377,
-0.030448615550994873,
-0.007060099858790636,
-0.04764891043305397,
0.01131939236074686,
0.0007969097350724041,
0.053800296038389206,
0.07622352987527847,
-0.07944231480360031,
0.010101311840116978,
-0.057927366346120834,
0.04787713661789894,
0.053292736411094666,
0.017422199249267578,
0.040096212178468704,
0.017402825877070427,
0.007534146774560213,
-0.0404096357524395,
0.00807233527302742,
-0.0003357990353833884,
0.046125028282403946,
-0.03611540421843529,
0.1201496347784996,
0.016690446063876152,
-0.026555880904197693,
0.03936026617884636,
-0.04193069040775299,
-0.016256488859653473,
0.019494494423270226,
-0.05566461384296417,
-0.014858787879347801,
-0.04288332164287567,
0.011351560242474079,
-0.06302426755428314,
-0.008363701403141022,
0.027939796447753906,
0.011552807874977589,
0.00920435506850481,
0.05879361182451248,
-0.05227261781692505,
-0.0568716824054718,
0.017599167302250862,
-0.017149068415164948,
0.03202752396464348,
0.006065228022634983,
-0.009280456230044365,
-0.0036207938101142645,
-0.009773657657206059,
-0.008055119775235653,
-0.004220296628773212,
-0.011748879216611385,
-0.025048905983567238,
-0.032890573143959045,
-0.02323063276708126,
-0.041993286460638046,
-0.02254924736917019,
-0.023566676303744316,
0.0252974983304739,
0.030643615871667862,
0.0050182524137198925,
0.012800109572708607,
-0.0019192562904208899,
0.03740455582737923,
-0.04410194233059883,
0.06429938971996307,
0.015284336172044277,
0.026099741458892822,
0.13515901565551758,
-0.028150446712970734,
0.06181316450238228,
0.08947176486253738,
0.027131900191307068,
0.00381881813518703,
-0.04610371217131615,
-0.07662296295166016,
0.006062416359782219,
-0.0019042566418647766,
-0.011236939579248428,
0.0331006795167923,
-0.03804249316453934,
0.014785559847950935,
-0.03024083748459816,
0.025071846321225166,
-0.044466160237789154,
0.060948505997657776,
0.03841225430369377,
0.007924413308501244,
0.013240060769021511,
-0.021899331361055374,
-0.0739084780216217,
0.003204943612217903,
0.007778727449476719,
-0.2910337448120117,
-0.03682689368724823,
0.0685407817363739,
0.04473961144685745,
0.006196672562509775,
-0.021155748516321182,
0.06085488200187683,
-0.018586697056889534,
-0.033508215099573135,
-0.027328068390488625,
-0.04216260090470314,
0.03296802192926407,
0.02656889520585537,
0.024298081174492836,
-0.015016607008874416,
-0.02986147627234459,
0.0333983451128006,
-0.01780661754310131,
-0.02257644385099411,
-0.049792852252721786,
0.008916628547012806,
-0.07485677301883698,
0.13460670411586761,
0.023393157869577408,
-0.12001354992389679,
0.07915150374174118,
0.020554378628730774,
0.0411839559674263,
-0.00657055526971817,
-0.0029330882243812084,
-0.026879405602812767,
-0.049999434500932693,
0.05018514767289162,
-0.05617912486195564,
0.035828568041324615,
0.0625028908252716,
-0.0458797812461853,
-0.0363575778901577,
0.007251687813550234,
0.02554917149245739,
-0.022619493305683136,
-0.04232359677553177,
-0.031003516167402267,
-0.017239397391676903,
0.05624352768063545,
-0.09107530862092972,
-0.008322041481733322,
-0.013655640184879303,
0.00017733511049300432,
-0.012618469074368477,
-0.03380448371171951,
0.010099670849740505,
-0.027252834290266037,
0.0267550777643919,
0.01177734974771738,
0.02655663713812828,
-0.015954425558447838,
0.0018104073824360967,
0.031098341569304466,
-0.07449161261320114,
0.002248241100460291,
-0.03851442411541939,
-0.031744733452796936,
0.08177585899829865,
0.0742463767528534
] |
PMID:8288 | Buffer catalysis of the racemization reaction of some 5-phenylhydantoins and its relation to the in vivo metabolism of ethotoin. | Evidence is presented to show that an optical isomer of 5-phenylhydantoin is subject to racemization (interconversion) in different buffer systems. With phosphate buffers in the pH range of 6.0-7.5, it appears that the buffer-catalyzed racemization reaction is due solely to catalysis by divalent phosphate (general base catalysis). Other buffers studied include arsenate, imidazole, triethanolamine, and pyrophosphate. When 5-phenylhydantoin, the N-de-ethylated metabolite of ethotoin, was administered to dogs in an earlier investigation, the observation was made that somewhat more than the theoretical quantity (50 mole percent of the dose) of the substances recovered from urine had the R-configuration. The principal metabolite was (R)-(-)-2-phenylhydantoic acid, formed stereo-specifically in a ring-opening reaction of (R)-5-phyenylhydantoin by dihydropyrimidinase (EC 3.5.2.2). The results of the present in vitro study support the hypothesis that in vivo the interconversion of the optical isomers of 5-phenylhydantoin can be catalyzed by buffering components of the mammalian physiological system, and that the catalytic activities of the endogenous buffer components can account for the racemization and ultimate metabolism of the (S)-isomer of 5-phenylhydantoin by dihydropyrimidinase. | Buffer catalysis of the racemization reaction of some 5-phenylhydantoins and its relation to the in vivo metabolism of ethotoin. Evidence is presented to show that an optical isomer of 5-phenylhydantoin is subject to racemization (interconversion) in different buffer systems. With phosphate buffers in the pH range of 6.0-7.5, it appears that the buffer-catalyzed racemization reaction is due solely to catalysis by divalent phosphate (general base catalysis). Other buffers studied include arsenate, imidazole, triethanolamine, and pyrophosphate. When 5-phenylhydantoin, the N-de-ethylated metabolite of ethotoin, was administered to dogs in an earlier investigation, the observation was made that somewhat more than the theoretical quantity (50 mole percent of the dose) of the substances recovered from urine had the R-configuration. The principal metabolite was (R)-(-)-2-phenylhydantoic acid, formed stereo-specifically in a ring-opening reaction of (R)-5-phyenylhydantoin by dihydropyrimidinase (EC 3.5.2.2). The results of the present in vitro study support the hypothesis that in vivo the interconversion of the optical isomers of 5-phenylhydantoin can be catalyzed by buffering components of the mammalian physiological system, and that the catalytic activities of the endogenous buffer components can account for the racemization and ultimate metabolism of the (S)-isomer of 5-phenylhydantoin by dihydropyrimidinase. | [
-0.047332845628261566,
-0.0044359625317156315,
-0.011902379803359509,
-0.0033249189145863056,
0.009822700172662735,
-0.04887208715081215,
0.00768771767616272,
0.0623556450009346,
-0.04996707662940025,
0.002041400410234928,
0.08132115751504898,
-0.06437952071428299,
0.01590767875313759,
0.06202656403183937,
-0.004466619808226824,
-0.05013168975710869,
-0.016666874289512634,
0.026745500043034554,
-0.0033317969646304846,
-0.019150428473949432,
-0.025888383388519287,
-0.05891424044966698,
0.007285403553396463,
-0.02134048379957676,
0.06254388391971588,
0.028208406642079353,
0.04055950418114662,
0.02610178478062153,
-0.0263503547757864,
-0.23868101835250854,
0.06020743399858475,
0.017643356695771217,
-0.029868097975850105,
-0.04764437675476074,
-0.015749961137771606,
0.036105576902627945,
-0.026655465364456177,
0.04034282639622688,
-0.07720110565423965,
0.09217265248298645,
0.04466237127780914,
0.011304941028356552,
-0.023631984367966652,
-0.003736581187695265,
0.012915111146867275,
0.045130446553230286,
-0.0779557079076767,
0.02668364904820919,
0.058403387665748596,
-0.041041579097509384,
0.001683627488091588,
0.012889273464679718,
0.021189719438552856,
0.05803048983216286,
0.011428321711719036,
-0.011145887896418571,
0.0746380016207695,
0.03965060040354729,
-0.043936364352703094,
0.015199052169919014,
0.002266982803121209,
0.06870137155056,
-0.15024548768997192,
0.06306803971529007,
0.037091661244630814,
0.008309213444590569,
0.038210075348615646,
-0.016899866983294487,
0.0195285864174366,
0.053556233644485474,
-0.08531685173511505,
0.0073602329939603806,
-0.030887581408023834,
-0.012520533055067062,
0.018041985109448433,
-0.02400599792599678,
0.02130708284676075,
-0.030781779438257217,
0.008959232829511166,
-0.005812964402139187,
-0.014903823845088482,
0.0250550527125597,
-0.023835444822907448,
0.01508047804236412,
0.04012466222047806,
0.023204118013381958,
0.010123040527105331,
-0.008128068409860134,
0.03846599534153938,
0.030260784551501274,
-0.022757936269044876,
-0.07657209783792496,
-0.02890884503722191,
-0.01995805837213993,
-0.03287190571427345,
0.04207853600382805,
0.021465271711349487,
-0.010482808575034142,
0.07454542070627213,
0.3809857666492462,
-0.015787217766046524,
0.01692866161465645,
-0.050074782222509384,
-0.006494926754385233,
0.006134547758847475,
-0.034442171454429626,
0.011047339998185635,
0.015184395015239716,
-0.004954912234097719,
0.008710073307156563,
0.014437595382332802,
0.03317499905824661,
0.05729590728878975,
-0.07264522463083267,
0.0007663886062800884,
0.011015971191227436,
-0.049788136035203934,
-0.007153183687478304,
0.011986171826720238,
0.05112362653017044,
-0.009443768300116062,
0.007563214749097824,
-0.007448721677064896,
-0.001278380979783833,
0.0068401312455534935,
0.006140834651887417,
-0.009259412996470928,
0.03270917758345604,
0.06438615173101425,
0.0017039997037500143,
0.013596033677458763,
-0.045153047889471054,
-0.01585330069065094,
0.002008214360103011,
-0.008050912991166115,
-0.033847395330667496,
0.044994600117206573,
-0.06326817721128464,
0.03820979967713356,
0.03800278529524803,
-0.027372265234589577,
-0.034671396017074585,
-0.03376173973083496,
-0.0837211161851883,
0.0012605627998709679,
0.12813381850719452,
-0.015767935663461685,
-0.018426064401865005,
-0.0355631522834301,
-0.006078607402741909,
0.016290100291371346,
0.03942245617508888,
-0.02709317021071911,
0.02079358883202076,
-0.04864083603024483,
0.01911112666130066,
0.001512788818217814,
-0.01794651336967945,
-0.016331825405359268,
-0.0058393958024680614,
-0.0460905022919178,
-0.017455825582146645,
-0.025638088583946228,
0.107979416847229,
-0.006244183052331209,
-0.031433261930942535,
-0.05246981978416443,
0.005174762569367886,
-0.04428118094801903,
-0.009359962306916714,
-0.019541332498192787,
-0.0550556406378746,
-0.009257658384740353,
0.03337563946843147,
-0.03817634657025337,
0.012783114798367023,
-0.028309835121035576,
0.03994803875684738,
-0.004201590549200773,
-0.0052253445610404015,
0.06422771513462067,
-0.004721884615719318,
-0.04774217680096626,
0.03295707330107689,
0.021854158490896225,
-0.001264895312488079,
-0.04511287063360214,
0.019833216443657875,
-0.03270559385418892,
0.06135471910238266,
0.004642089828848839,
-0.008848176337778568,
-0.0717272087931633,
0.03620130196213722,
-0.01277654618024826,
-0.011335421353578568,
-0.07055500149726868,
-0.020393390208482742,
0.04092418774962425,
-0.04187818989157677,
0.08692234754562378,
0.04902687668800354,
-0.013670367188751698,
0.047526467591524124,
-0.00663920259103179,
-0.0059498390182852745,
-0.021662985906004906,
0.011417032219469547,
0.035238273441791534,
0.0029222152661532164,
-0.02853512018918991,
0.01431052666157484,
-0.040471483021974564,
0.00034733014763332903,
-0.01926828920841217,
0.0010360704036429524,
-0.0157985407859087,
-0.004131506662815809,
0.058537501841783524,
-0.011258050799369812,
0.04679708555340767,
-0.08140958100557327,
-0.03519797697663307,
-0.3123173415660858,
-0.06747712939977646,
-0.005554558243602514,
-0.010127022862434387,
0.08776481449604034,
-0.024486904963850975,
0.022963162511587143,
0.0008948217728175223,
0.0040102768689394,
-0.025282537564635277,
0.03822128847241402,
0.07171448320150375,
0.00906404945999384,
-0.04026368632912636,
-0.031305648386478424,
0.015652615576982498,
-0.019992534071207047,
-0.047888416796922684,
0.06020335853099823,
-0.037965744733810425,
-0.0006234190077520907,
-0.01030874252319336,
0.0049231164157390594,
0.05263686925172806,
0.08741491287946701,
0.032479993999004364,
0.10607127845287323,
0.038885533809661865,
0.014561280608177185,
-0.026646679267287254,
-0.01857900246977806,
0.055899057537317276,
-0.0384477898478508,
-0.09808368980884552,
-0.03523007407784462,
-0.015901517122983932,
0.0014509615721181035,
-0.03673627972602844,
-0.008350888267159462,
-0.0025028104428201914,
-0.021065635606646538,
-0.004124555271118879,
-0.039776671677827835,
-0.0949014350771904,
-0.03388971462845802,
0.008070162497460842,
-0.03605515509843826,
-0.034272145479917526,
0.030852127820253372,
0.011126913130283356,
0.03432132303714752,
0.05810573697090149,
0.07285789400339127,
-0.033166881650686264,
0.0022985567338764668,
-0.03296208381652832,
-0.06203572824597359,
0.06541626155376434,
-0.030267536640167236,
-0.04264719411730766,
0.01980540156364441,
-0.0028356595430523157,
0.024198779836297035,
0.020998790860176086,
0.004720860160887241,
-0.007426690775901079,
0.01767568290233612,
-0.008480879478156567,
0.08153087645769119,
0.04966067150235176,
0.05371642857789993,
0.14236551523208618,
-0.05267053097486496,
0.04195330664515495,
0.029695026576519012,
0.029022570699453354,
-0.03593346104025841,
-0.011554709635674953,
-0.0875018984079361,
0.02988896705210209,
-0.009415878914296627,
-0.007150535471737385,
0.05068650469183922,
-0.02144172601401806,
-0.01757088117301464,
-0.007861224934458733,
0.003793912474066019,
0.041995204985141754,
0.05138108879327774,
0.055603865534067154,
0.02500242553651333,
-0.0010393508709967136,
-0.04339832440018654,
-0.09079335629940033,
0.04144930839538574,
0.05743648111820221,
-0.28707897663116455,
-0.0029266641940921545,
-0.016826996579766273,
0.012733899056911469,
0.02927917242050171,
0.019067805260419846,
0.050111398100852966,
-0.008911830373108387,
-0.04776030033826828,
0.015267572365701199,
-0.025816498324275017,
0.04530123621225357,
0.05766516923904419,
0.04921044036746025,
-0.01570083573460579,
0.034059781581163406,
0.0380559004843235,
-0.03642900288105011,
-0.030430082231760025,
-0.019227009266614914,
-0.07837036997079849,
-0.023830292746424675,
0.13671736419200897,
0.010038156062364578,
-0.015162275172770023,
0.0674530416727066,
0.016568094491958618,
0.022193435579538345,
-0.014910218305885792,
0.0002784443786367774,
-0.04378550499677658,
0.02994670160114765,
0.025161435827612877,
-0.05505000799894333,
0.02688208408653736,
0.014500237070024014,
-0.013628087937831879,
0.002695785602554679,
0.0005454447236843407,
0.07302716374397278,
-0.009817719459533691,
-0.024154512211680412,
-0.04899398237466812,
-0.048538483679294586,
0.04711069539189339,
-0.03415565565228462,
-0.0619441382586956,
-0.030836179852485657,
0.000019194814740330912,
0.02046578750014305,
-0.036879029124975204,
-0.03021143190562725,
0.006226883269846439,
0.025546958670020103,
0.011072744615375996,
0.052593864500522614,
-0.02559777721762657,
-0.02757132425904274,
0.025515271350741386,
-0.019777115434408188,
0.0060246167704463005,
-0.017923178151249886,
-0.032743752002716064,
0.06938478350639343,
-0.0012574237771332264
] |
PMID:8289 | 5,5-Bis(3-hydroxyphenyl)hydantoin, a minor metabolite of diphenylhydantoin (dilantin) in the rat and human. | 5,5-Bis(4-hydroxyphenyl) hydantoin has been identified as a minor metabolite of diphenylhydantoin (DPH) in the rat and human. This metabolite was synthesized in the laboratory from 4,4'-dihydroxybenzophenone. Gas chromatographic and mass spectrometric comparison of the permethylated synthetic compound with the permethylated derivative of the metabolite obtained from biological sources showed that they were identical. The metabolite was excreted as a glucuronide and accounted for about 1% of the total hydroxylated metabolites of DPH in human urine and rat bile. When the synthetic standard was added to the recirculating perfusate of the isolated perfused rat liver, a monoglucuronide, a trihydroxy-DPH glucuronide, and a dihydroxymethoxy-DPH glucuronide were identified in the bile. | 5,5-Bis(3-hydroxyphenyl)hydantoin, a minor metabolite of diphenylhydantoin (dilantin) in the rat and human. 5,5-Bis(4-hydroxyphenyl) hydantoin has been identified as a minor metabolite of diphenylhydantoin (DPH) in the rat and human. This metabolite was synthesized in the laboratory from 4,4'-dihydroxybenzophenone. Gas chromatographic and mass spectrometric comparison of the permethylated synthetic compound with the permethylated derivative of the metabolite obtained from biological sources showed that they were identical. The metabolite was excreted as a glucuronide and accounted for about 1% of the total hydroxylated metabolites of DPH in human urine and rat bile. When the synthetic standard was added to the recirculating perfusate of the isolated perfused rat liver, a monoglucuronide, a trihydroxy-DPH glucuronide, and a dihydroxymethoxy-DPH glucuronide were identified in the bile. | [
-0.03992481902241707,
-0.007107493467628956,
-0.004217205103486776,
0.007863921113312244,
0.02649102546274662,
-0.02137092500925064,
0.0021197006572037935,
0.04032701998949051,
-0.05235766991972923,
-0.02701311931014061,
0.010397582314908504,
-0.05248213931918144,
0.06630933284759521,
-0.026232725009322166,
-0.05490444228053093,
-0.058459892868995667,
0.04155963659286499,
-0.036673303693532944,
-0.029153509065508842,
-0.0005504149594344199,
0.044942934066057205,
-0.044363994151353836,
0.031453441828489304,
0.0202683974057436,
0.033761851489543915,
0.034915272146463394,
0.06170260161161423,
0.04274829849600792,
-0.06899644434452057,
-0.19111262261867523,
0.05398290604352951,
0.04495537281036377,
-0.0006982036284171045,
-0.08189752697944641,
-0.04730091989040375,
0.006929469760507345,
-0.01753617636859417,
0.06715985387563705,
0.022996069863438606,
0.03339094668626785,
0.014765482395887375,
0.02342781238257885,
-0.023937620222568512,
-0.020846936851739883,
0.03892943635582924,
0.024101780727505684,
-0.09466855973005295,
-0.005151194520294666,
0.09415037930011749,
0.0064632343128323555,
-0.002790751401335001,
-0.018179992213845253,
0.049581002444028854,
0.037862081080675125,
0.02358480542898178,
-0.0206975769251585,
0.05801190063357353,
0.011517156846821308,
-0.06316972523927689,
0.05733068287372589,
-0.015554006211459637,
0.08039478212594986,
-0.12750540673732758,
0.06015554070472717,
-0.004681955557316542,
0.018764058127999306,
-0.003979355096817017,
-0.05098338797688484,
0.05348043888807297,
0.0377085916697979,
-0.05925707519054413,
-0.01997336931526661,
-0.03262731060385704,
-0.010276589542627335,
-0.03003016486763954,
-0.04512988030910492,
0.011816024780273438,
-0.06466124206781387,
0.014749524183571339,
0.0020188235212117434,
0.0386786162853241,
0.06526213139295578,
0.0272612813860178,
0.0033276674803346395,
0.04954281449317932,
-0.022026302292943,
0.025712842121720314,
0.020381217822432518,
0.015852095559239388,
-0.006801520939916372,
-0.015245258808135986,
-0.03672155365347862,
0.006884602829813957,
-0.015050038695335388,
-0.061622750014066696,
-0.008433505892753601,
0.009015131741762161,
-0.010919044725596905,
-0.01704205572605133,
0.355951189994812,
-0.027995949611067772,
0.024546276777982712,
-0.027093131095170975,
-0.0025459446478635073,
0.00977811124175787,
-0.006264912895858288,
-0.0028799024876207113,
0.0032925058621913195,
0.03380357101559639,
0.01137646846473217,
0.025008132681250572,
0.031269341707229614,
0.07398997247219086,
-0.060595836490392685,
-0.03167033568024635,
-0.04391862452030182,
0.013417803682386875,
-0.027124034240841866,
-0.01833927258849144,
0.0573657862842083,
0.02050188183784485,
0.030682146549224854,
0.045555051416158676,
0.004613851197063923,
0.014657855965197086,
-0.007601196877658367,
-0.03511059656739235,
0.05549895763397217,
0.0717768594622612,
0.05491840839385986,
0.04646500572562218,
-0.024545323103666306,
0.007179873064160347,
0.011398764327168465,
0.054554980248212814,
0.0023099943064153194,
0.02653336711227894,
-0.07678984850645065,
0.002508574863895774,
-0.004929063841700554,
-0.08394993841648102,
-0.0426570288836956,
-0.04327703267335892,
-0.04550671577453613,
-0.10461703687906265,
0.12695074081420898,
0.010160403326153755,
-0.026613129302859306,
0.017632676288485527,
0.020206233486533165,
0.0020249616354703903,
0.05912178009748459,
-0.0331861674785614,
0.02626803331077099,
-0.05586835741996765,
0.018431801348924637,
0.029721170663833618,
0.009241975843906403,
-0.02765977755188942,
0.023410681635141373,
0.0019170183222740889,
-0.002029538620263338,
-0.034919049590826035,
0.09686640650033951,
-0.03817944973707199,
-0.05078919604420662,
-0.042337123304605484,
0.05003991350531578,
0.010815639980137348,
0.02188827469944954,
0.006996039301156998,
-0.00867842510342598,
-0.031073467805981636,
0.03360036760568619,
-0.024298273026943207,
-0.027504151687026024,
0.030773652717471123,
0.033550456166267395,
-0.002704926300793886,
0.00010867645323742181,
0.047979000955820084,
0.017359057441353798,
-0.07802148908376694,
-0.027353864163160324,
0.03562641143798828,
-0.0265068169683218,
-0.0384388230741024,
0.01852724514901638,
-0.042169731110334396,
0.066377654671669,
-0.015145345591008663,
0.021368078887462616,
-0.06280394643545151,
0.013840683735907078,
-0.006652094889432192,
-0.0361395962536335,
-0.10416996479034424,
0.05814841389656067,
0.01350259780883789,
-0.05775012820959091,
0.07588634639978409,
0.004483074881136417,
0.028698930516839027,
0.06249266117811203,
0.02390061691403389,
0.015888061374425888,
0.025327835232019424,
-0.005622234661132097,
0.004615927580744028,
-0.024750495329499245,
-0.02807031385600567,
0.06033308804035187,
-0.01063541416078806,
0.020692769438028336,
0.0171811580657959,
0.07895324379205704,
-0.015075314790010452,
-0.004161025397479534,
0.005118727218359709,
0.027600359171628952,
0.0010093344608321786,
-0.07216779887676239,
-0.03606318682432175,
-0.293671578168869,
-0.04865246266126633,
0.017444979399442673,
-0.01875677891075611,
0.08620187640190125,
-0.027874577790498734,
-0.006766297854483128,
0.002787112956866622,
-0.0031416155397892,
-0.02287227474153042,
0.04881234094500542,
0.1020502820611,
-0.06164892390370369,
-0.028037458658218384,
-0.04289733245968819,
0.05154767259955406,
0.027269503101706505,
-0.019312750548124313,
0.07617352157831192,
-0.035990774631500244,
-0.007399957161396742,
-0.04495751112699509,
0.02526872232556343,
0.0033567354548722506,
0.07076903432607651,
-0.04010611027479172,
0.14062151312828064,
0.031483810395002365,
-0.050556957721710205,
-0.020387805998325348,
0.02565394528210163,
0.04616590961813927,
-0.008956022560596466,
-0.13235104084014893,
0.03313138335943222,
-0.021646080538630486,
0.021743522956967354,
-0.034119926393032074,
-0.00002473993481544312,
-0.05048248544335365,
-0.06525902450084686,
0.0058417534455657005,
-0.012978417798876762,
-0.10955453664064407,
-0.043253134936094284,
-0.002302006119862199,
-0.042223770171403885,
-0.03828642889857292,
0.05397004261612892,
-0.04750198870897293,
0.0183724332600832,
0.03938206285238266,
0.0324949249625206,
0.006412425544112921,
0.028399605304002762,
0.0021175388246774673,
-0.0448080413043499,
-0.04198639839887619,
-0.06332173943519592,
-0.02145574241876602,
0.01783788949251175,
0.014702430926263332,
0.014697419479489326,
-0.02172740548849106,
-0.04554244875907898,
-0.007570791058242321,
-0.006716476287692785,
-0.03991248831152916,
0.0650726780295372,
0.06299321353435516,
0.04623289033770561,
0.10830822587013245,
-0.04837958514690399,
0.0822414979338646,
-0.003923106472939253,
0.0072884587571024895,
-0.014451198279857635,
-0.017213210463523865,
-0.07761236280202866,
0.043926067650318146,
0.03583957254886627,
-0.04134419187903404,
0.008423364721238613,
0.015383951365947723,
-0.013384563848376274,
-0.005151872057467699,
-0.0025761262513697147,
-0.018158409744501114,
0.0767289474606514,
0.04329220950603485,
0.030267637223005295,
0.0016696553211659193,
0.016212329268455505,
-0.06965944170951843,
0.022032391279935837,
0.017544500529766083,
-0.2638421952724457,
0.02447367273271084,
-0.027109971269965172,
0.014398814179003239,
0.04654146730899811,
-0.01147537399083376,
0.03104470856487751,
-0.01738167740404606,
-0.10118094831705093,
0.00621509924530983,
0.028508268296718597,
0.007737081963568926,
0.08220452070236206,
0.011489825323224068,
-0.03294961154460907,
-0.014474010095000267,
0.04084981977939606,
0.029206570237874985,
-0.05275659263134003,
0.03470006212592125,
-0.019345000386238098,
-0.028725620359182358,
0.08750339597463608,
-0.0056297667324543,
-0.026781950145959854,
0.03950057178735733,
-0.013786634430289268,
0.001851303968578577,
-0.0750703290104866,
0.05850750580430031,
-0.0706053301692009,
0.009380282834172249,
0.0954059436917305,
-0.06597903370857239,
0.017242325469851494,
0.0392780564725399,
-0.04742893576622009,
0.006546346470713615,
0.03264060989022255,
0.044912923127412796,
-0.01970364898443222,
0.007567235734313726,
-0.055238887667655945,
0.028753595426678658,
0.05807334929704666,
-0.058131515979766846,
-0.029587743803858757,
-0.028679456561803818,
0.047416653484106064,
0.022710170596837997,
-0.018535790964961052,
0.000652938848361373,
-0.015885762870311737,
0.0038945369888097048,
0.006959989201277494,
0.03866845369338989,
0.018724944442510605,
-0.013927417807281017,
-0.029276259243488312,
-0.05891329422593117,
-0.044403236359357834,
-0.021477781236171722,
0.0014458044897764921,
0.0807659775018692,
0.005047884304076433
] |
PMID:8290 | Metabolism of dihalomethanes to formaldehyde and inorganic halide. I. In vitro studies. | Metabolism of dihalomethanes by rat liver cytosol fractions yielded formaldehyde and inorganic halide as products. Loss of metabolic activity resulting from dialysis of the cytosol was restored with glutathione. Cysteine could not substitute for GSH. No other cofactor was found to be required for activity. The optimum conditions for this biotransformation with respect to time, temperature, protein concentration, and pH were determined. Rates of metabolism of dihalomethanes showed the following order: CH2i2 greater than CH2Br2 congruent to CH2BrCi greater than CH2Ci2. Administration of the enzyme inducer, phenobarbital, to rats did not alter this metabolic pathway nor did repeated administration of CH2Br2 or CH2Ci2 change the rate of metabolism. The enzyme catalyzing this reaction was localized in the liver. Compounds known to serve as substrates for various GSH transferases inhibited the reaction as did those capable of interacting with sulfhydryl groups. | Metabolism of dihalomethanes to formaldehyde and inorganic halide. I. In vitro studies. Metabolism of dihalomethanes by rat liver cytosol fractions yielded formaldehyde and inorganic halide as products. Loss of metabolic activity resulting from dialysis of the cytosol was restored with glutathione. Cysteine could not substitute for GSH. No other cofactor was found to be required for activity. The optimum conditions for this biotransformation with respect to time, temperature, protein concentration, and pH were determined. Rates of metabolism of dihalomethanes showed the following order: CH2i2 greater than CH2Br2 congruent to CH2BrCi greater than CH2Ci2. Administration of the enzyme inducer, phenobarbital, to rats did not alter this metabolic pathway nor did repeated administration of CH2Br2 or CH2Ci2 change the rate of metabolism. The enzyme catalyzing this reaction was localized in the liver. Compounds known to serve as substrates for various GSH transferases inhibited the reaction as did those capable of interacting with sulfhydryl groups. | [
0.006299923174083233,
0.006914222612977028,
-0.008639929816126823,
-0.006920211482793093,
0.055560462176799774,
-0.0470125749707222,
-0.004815472289919853,
0.0014028067234903574,
-0.053455933928489685,
0.01174241490662098,
0.031177351251244545,
-0.06636323034763336,
0.0023923919070512056,
0.027024045586586,
-0.011655288748443127,
-0.014023598283529282,
0.026249483227729797,
-0.02423170395195484,
-0.04110197722911835,
-0.009232004173099995,
-0.038078971207141876,
0.05051877722144127,
0.05193360149860382,
0.023303590714931488,
0.020166359841823578,
-0.005536824930459261,
-0.03657395765185356,
0.057178858667612076,
-0.08254196494817734,
-0.20119883120059967,
-0.04234269633889198,
0.04702729731798172,
0.00640589464455843,
-0.018333997577428818,
-0.026975762099027634,
-0.005778577644377947,
-0.00032366151572205126,
0.03468819707632065,
-0.018179330974817276,
0.03506573289632797,
0.08167928457260132,
0.06482347100973129,
0.028205569833517075,
0.006059946026653051,
0.00925519596785307,
0.04215824231505394,
-0.04140348732471466,
-0.0007822613115422428,
0.07986349612474442,
-0.02335716225206852,
0.03359954059123993,
-0.02128453180193901,
0.027076033875346184,
0.04765298217535019,
-0.0215256717056036,
-0.02029654197394848,
0.056275174021720886,
0.0404663048684597,
-0.0012502721510827541,
0.013640318065881729,
-0.033573899418115616,
0.0026462054811418056,
-0.13077180087566376,
0.0459371954202652,
0.02827432192862034,
-0.004345492459833622,
-0.04732050374150276,
-0.06557948142290115,
0.04364790767431259,
0.06306944042444229,
-0.07711518555879593,
-0.034101780503988266,
0.026832880452275276,
0.0014685356291010976,
0.03429068997502327,
-0.006197601091116667,
0.008947997353971004,
-0.021290376782417297,
0.01913166232407093,
0.03785751014947891,
0.019112611189484596,
0.007520620711147785,
-0.0012419152772054076,
-0.04484331235289574,
0.0036122112069278955,
-0.02834838256239891,
0.005091742612421513,
-0.013130011968314648,
0.00380588392727077,
0.016569361090660095,
-0.04801042750477791,
-0.1013435423374176,
0.026835201308131218,
-0.021056119352579117,
-0.08317455649375916,
0.043155718594789505,
0.031284186989068985,
0.018694225698709488,
0.03207659721374512,
0.3690318763256073,
0.01643897406756878,
0.05779629945755005,
-0.07632158696651459,
-0.020843978971242905,
0.0029498066287487745,
-0.02074451744556427,
-0.04430503398180008,
0.004873688332736492,
0.008433712646365166,
-0.005915213841944933,
0.08396889269351959,
0.01040448434650898,
0.042432792484760284,
-0.019088605418801308,
-0.0795706957578659,
0.024903571233153343,
0.01525023765861988,
0.008843216113746166,
-0.01937193050980568,
0.03346835449337959,
0.00534605560824275,
-0.00933622196316719,
0.054108280688524246,
0.006555907893925905,
0.03129284456372261,
-0.017770707607269287,
0.007046417333185673,
0.05084608867764473,
0.03846447169780731,
0.08959516137838364,
0.03875270485877991,
-0.019144870340824127,
0.017852896824479103,
0.03057979606091976,
-0.017057735472917557,
-0.014456401579082012,
0.027692625299096107,
-0.0864790678024292,
-0.005763818509876728,
-0.04402177035808563,
-0.019365713000297546,
-0.019935162737965584,
-0.05007840692996979,
-0.047447413206100464,
-0.049182504415512085,
0.05717053636908531,
0.01906907930970192,
-0.040669798851013184,
0.0023949462920427322,
-0.0015392025234177709,
0.013468352146446705,
0.034550707787275314,
-0.007386714685708284,
0.01450362242758274,
-0.026983486488461494,
0.029720518738031387,
0.015394062735140324,
-0.00035214051604270935,
0.01638602465391159,
-0.019933531060814857,
-0.03227256238460541,
-0.011150862090289593,
0.01168606523424387,
0.11125679314136505,
0.010677260346710682,
0.03742270544171333,
-0.05319726839661598,
0.0858059749007225,
0.06049887835979462,
0.03230815753340721,
0.04084162414073944,
0.006271327845752239,
0.01939128339290619,
-0.017193544656038284,
-0.027602113783359528,
0.016457006335258484,
0.07483217865228653,
0.08607570827007294,
0.03684953600168228,
0.0439816452562809,
0.040015142410993576,
0.0019171569729223847,
-0.06831681728363037,
0.05410250276327133,
0.02824227325618267,
-0.009423458017408848,
-0.04685362055897713,
0.008593747392296791,
-0.056474469602108,
0.05709616094827652,
-0.02069864608347416,
0.021067775785923004,
-0.13208283483982086,
-0.004975639283657074,
-0.03005191870033741,
-0.0016525296960026026,
-0.08716411888599396,
-0.017212366685271263,
-0.009444848634302616,
-0.0329938642680645,
0.1118781641125679,
0.04011902958154678,
0.024354474619030952,
0.04688068851828575,
0.0097229378297925,
0.006148174870759249,
-0.04201972112059593,
0.018132256343960762,
0.04411051422357559,
0.026124877855181694,
-0.04650940001010895,
-0.01595262624323368,
-0.048982154577970505,
0.03058352693915367,
0.003270860994234681,
-0.0052832202054560184,
0.010127448476850986,
-0.005899626296013594,
0.0440652035176754,
0.009973140433430672,
0.040825922042131424,
-0.07885611802339554,
-0.07888249307870865,
-0.3315586745738983,
-0.00986053328961134,
0.025348737835884094,
0.019722996279597282,
0.044859953224658966,
-0.017060412093997,
0.02110772393643856,
-0.006288347765803337,
-0.05318757891654968,
-0.044893473386764526,
0.04005628824234009,
0.04429040476679802,
-0.04447672888636589,
-0.027718644589185715,
-0.029881881549954414,
0.046526018530130386,
0.02945491299033165,
-0.026560897007584572,
0.008854692801833153,
-0.0019145815167576075,
0.019965430721640587,
-0.06881049275398254,
0.03090176172554493,
-0.021020807325839996,
0.05485520884394646,
-0.011127496138215065,
0.11885634809732437,
0.016678839921951294,
0.014565028250217438,
0.01668657548725605,
0.031523656100034714,
0.040842510759830475,
-0.061712246388196945,
-0.11459805816411972,
-0.05071898177266121,
-0.09860909730195999,
0.008437483571469784,
-0.04323742911219597,
0.04878070950508118,
-0.01234479621052742,
-0.04440172761678696,
0.004754121880978346,
-0.05742723494768143,
-0.023799315094947815,
-0.03850042074918747,
0.028896624222397804,
-0.05527332052588463,
-0.03723554313182831,
-0.02982381358742714,
-0.07530434429645538,
0.026926303282380104,
0.06714466959238052,
0.006999677047133446,
-0.056221045553684235,
0.052048712968826294,
-0.020992225036025047,
-0.029014213010668755,
-0.018008675426244736,
-0.11649775505065918,
-0.03576567396521568,
-0.006907580886036158,
0.031501926481723785,
0.0037012549582868814,
0.01634863018989563,
0.02818158082664013,
-0.02465733513236046,
-0.038545574992895126,
-0.025438163429498672,
0.0395483672618866,
0.060293395072221756,
-0.008061018772423267,
0.10039835423231125,
-0.033073872327804565,
-0.032272566109895706,
0.029593294486403465,
0.0114541444927454,
-0.09036338329315186,
-0.03753666952252388,
-0.0723567008972168,
0.028377754613757133,
-0.03274919092655182,
-0.008992714807391167,
0.060774289071559906,
-0.011735104024410248,
-0.030178450047969818,
0.02849378064274788,
0.010094711557030678,
-0.01722375862300396,
0.024835048243403435,
0.02967027574777603,
0.007914540357887745,
0.00908462144434452,
-0.05317576229572296,
-0.035975415259599686,
0.05648108199238777,
0.009806698188185692,
-0.2340581715106964,
-0.011925864964723587,
0.012134161777794361,
-0.005535219796001911,
-0.004818013869225979,
-0.06686123460531235,
0.0063369739800691605,
-0.01924741081893444,
-0.032746437937021255,
0.009719595313072205,
0.025563504546880722,
0.048881884664297104,
0.05029520392417908,
0.03793565183877945,
0.0457710362970829,
-0.008548498153686523,
0.01137572806328535,
-0.01761467196047306,
-0.039758432656526566,
-0.02340780757367611,
-0.03673596307635307,
-0.031600914895534515,
0.172928586602211,
0.04734855890274048,
-0.011453652754426003,
0.02427292987704277,
0.012664341367781162,
-0.006469413172453642,
0.0054559772834181786,
0.057764530181884766,
0.0018949001096189022,
-0.02166639268398285,
0.08761890977621078,
-0.02330949157476425,
0.04448343813419342,
0.04432705417275429,
-0.038840580731630325,
0.015546858310699463,
-0.05245034024119377,
-0.004233140032738447,
-0.04213625565171242,
0.03611962869763374,
-0.03890639543533325,
-0.011332427151501179,
0.0811317190527916,
0.006865713745355606,
-0.034511834383010864,
-0.048311907798051834,
0.03076484054327011,
0.028825318440794945,
-0.04319582134485245,
-0.005030680913478136,
0.014682789333164692,
0.029935799539089203,
-0.005348585546016693,
0.0007325640763156116,
-0.01789793185889721,
-0.015564408153295517,
0.030122045427560806,
0.02179790660738945,
-0.012434705160558224,
0.03763994574546814,
-0.03279319033026695,
0.06884605437517166,
0.051093779504299164
] |
PMID:8291 | Metabolism of 2,4,5,2',5'-pentachlorobiphenyl in the rat. Qualitative and quantitative aspects. | The metabolism of 2,4,5,2',5'-pentachloro [14C] biphenyl (5-CB) was studied in the male rat. Following iv administration of 5-CB (0.6 mg/kg), 84% of the total dose was excreted within 7 days and 89% of the radioactivity excreted was in the form of 5-CB metabolites. Mass spectral and proton nuclear magnetic resonance analysis or chemical methods in conjunction with mass spectral analysis were used to identify the metabolites. The major metabolite was identified as 3'-hydroxy 5-CB. One minor metabolite was identified as the 3',4'-dihydrodiol of 5-CB and a second minor metabolite was tentatively identified as 3',4'-dihydroxy-5-CB. The relative amounts of 5-CB and its metabolites excreted in the urine and feces were also determined. | Metabolism of 2,4,5,2',5'-pentachlorobiphenyl in the rat. Qualitative and quantitative aspects. The metabolism of 2,4,5,2',5'-pentachloro [14C] biphenyl (5-CB) was studied in the male rat. Following iv administration of 5-CB (0.6 mg/kg), 84% of the total dose was excreted within 7 days and 89% of the radioactivity excreted was in the form of 5-CB metabolites. Mass spectral and proton nuclear magnetic resonance analysis or chemical methods in conjunction with mass spectral analysis were used to identify the metabolites. The major metabolite was identified as 3'-hydroxy 5-CB. One minor metabolite was identified as the 3',4'-dihydrodiol of 5-CB and a second minor metabolite was tentatively identified as 3',4'-dihydroxy-5-CB. The relative amounts of 5-CB and its metabolites excreted in the urine and feces were also determined. | [
-0.023829422891139984,
-0.010632702149450779,
-0.0639452263712883,
-0.035037532448768616,
0.04248809814453125,
0.029879365116357803,
0.0181434229016304,
0.03411611169576645,
-0.05811180919408798,
-0.032017722725868225,
0.013171986676752567,
-0.11327013373374939,
0.00393232936039567,
-0.011870662681758404,
-0.0594814196228981,
-0.029735416173934937,
0.022928008809685707,
-0.047124963253736496,
-0.009967334568500519,
0.028223415836691856,
-0.001777999335899949,
-0.042362213134765625,
0.02689562924206257,
-0.008292498998343945,
0.017006773501634598,
0.029805762693285942,
-0.001649666577577591,
0.028341349214315414,
-0.1050783097743988,
-0.18684086203575134,
0.032311420887708664,
0.08228835463523865,
0.016165615990757942,
-0.04760267212986946,
-0.0018558681476861238,
-0.0089469775557518,
0.03155718371272087,
0.05867595225572586,
-0.04085738584399223,
0.05650951340794563,
0.024809764698147774,
0.034353144466876984,
0.036783892661333084,
0.012169182300567627,
0.018493331968784332,
0.008116544224321842,
-0.06303568929433823,
0.030998673290014267,
0.12146155536174774,
-0.008086963556706905,
-0.003257547738030553,
-0.005221144296228886,
0.0053532239980995655,
0.03337939456105232,
-0.01646498031914234,
-0.001560799079015851,
0.06813729554414749,
0.003979989793151617,
-0.023809535428881645,
0.07228190451860428,
-0.013978364877402782,
0.05026354640722275,
-0.14060603082180023,
0.06641501188278198,
0.0255443062633276,
0.03583772853016853,
-0.05043651908636093,
-0.06127428263425827,
0.057256124913692474,
0.036448098719120026,
-0.0630028247833252,
-0.024608617648482323,
-0.03477278724312782,
-0.036221928894519806,
-0.014025733806192875,
-0.01630364917218685,
-0.031139494851231575,
-0.04782376438379288,
-0.009031048975884914,
0.00357409892603755,
0.0001842251658672467,
0.02776850387454033,
0.034372784197330475,
-0.00613904045894742,
0.024560794234275818,
-0.020706022158265114,
-0.029941512271761894,
-0.028467783704400063,
0.025042012333869934,
0.06976035982370377,
-0.0025386710185557604,
-0.051887061446905136,
0.004488152451813221,
-0.0238849688321352,
-0.052178818732500076,
-0.026911618188023567,
0.04299694299697876,
-0.015529284253716469,
-0.011785438284277916,
0.31002354621887207,
0.0216287299990654,
0.022794757038354874,
-0.042087480425834656,
-0.004812422674149275,
-0.01814894936978817,
-0.022515680640935898,
0.016665300354361534,
0.03767484053969383,
0.005600736476480961,
-0.025633016601204872,
0.046816080808639526,
0.037102047353982925,
0.05648732930421829,
-0.10801856219768524,
-0.053262416273355484,
-0.03696449473500252,
0.018148677423596382,
-0.01410000305622816,
-0.01616291143000126,
0.037317316979169846,
0.021415874361991882,
0.007309654261916876,
0.04326567053794861,
0.06561966240406036,
0.04468490183353424,
0.0049290889874100685,
-0.0049028354696929455,
0.06964391469955444,
0.038398317992687225,
0.045102767646312714,
0.017028192058205605,
0.03510241582989693,
0.046484727412462234,
0.007182684727013111,
-0.027514485642313957,
-0.009619941003620625,
0.08586784452199936,
-0.004209044389426708,
0.026467323303222656,
0.007448273710906506,
0.005760702304542065,
-0.055505357682704926,
-0.015876736491918564,
-0.042534857988357544,
-0.07360618561506271,
0.11200147867202759,
0.06214204058051109,
-0.03128357604146004,
-0.005709085147827864,
-0.02281859517097473,
0.012710357084870338,
0.044109195470809937,
-0.018148433417081833,
0.04590767249464989,
-0.02297343872487545,
0.04253743588924408,
-0.006492253392934799,
0.024351559579372406,
-0.019493959844112396,
0.02813868597149849,
-0.04056047648191452,
0.002070110756903887,
-0.07295141369104385,
0.08023511618375778,
0.01805480755865574,
0.01984570361673832,
-0.06504704803228378,
0.02397516556084156,
0.06113448366522789,
-0.0299332607537508,
0.004926842171698809,
0.026124147698283195,
-0.04264925420284271,
0.005085624288767576,
-0.051079992204904556,
-0.04442255198955536,
0.042839549481868744,
0.04145458713173866,
-0.00935015082359314,
-0.01075990591198206,
0.023769153282046318,
-0.034342147409915924,
-0.014875142835080624,
0.04618426412343979,
-0.01190697681158781,
0.04774412885308266,
-0.024516748264431953,
-0.044152773916721344,
0.01936362124979496,
0.08080068230628967,
-0.0448201484978199,
-0.00011787586845457554,
-0.05285058543086052,
-0.010150505229830742,
-0.04775931313633919,
-0.0553809255361557,
-0.1016656905412674,
0.03915197774767876,
-0.011676108464598656,
-0.0295285414904356,
0.11395908147096634,
0.011099662631750107,
-0.0025955878663808107,
0.049284785985946655,
0.03874024748802185,
0.046737588942050934,
-0.00754537433385849,
0.0016799047589302063,
0.05706162750720978,
-0.006960845086723566,
0.00247377110645175,
-0.01695634424686432,
-0.025057723745703697,
-0.0034373137168586254,
0.02506532520055771,
0.01036379300057888,
-0.026141218841075897,
-0.007333443965762854,
0.07169796526432037,
-0.013613905757665634,
0.013134057633578777,
-0.064646415412426,
-0.02637903392314911,
-0.2863914668560028,
-0.03602757677435875,
0.06128459796309471,
0.011497843079268932,
0.13094405829906464,
-0.050408534705638885,
-0.02341645583510399,
-0.05912872776389122,
-0.0185206588357687,
-0.026665788143873215,
0.012779762968420982,
-0.004109260626137257,
0.00330903846770525,
-0.05220392346382141,
-0.030617428943514824,
0.004922079853713512,
0.08148349821567535,
-0.01753140427172184,
0.02733631804585457,
-0.0505080446600914,
0.0009636476752348244,
-0.061089061200618744,
-0.017546990886330605,
-0.012860571965575218,
0.10802137851715088,
-0.024447888135910034,
0.10451377183198929,
-0.04712147265672684,
-0.04771425575017929,
0.010198600590229034,
0.05059105157852173,
0.023396141827106476,
0.014088280498981476,
-0.08805045485496521,
0.025281310081481934,
-0.057261839509010315,
-0.014216791838407516,
-0.00892073567956686,
-0.005818168632686138,
0.004079833626747131,
-0.050053056329488754,
0.004167549777776003,
-0.02683725208044052,
-0.10941700637340546,
-0.07366377860307693,
0.018431952223181725,
-0.03214530274271965,
-0.030260615050792694,
0.006794940214604139,
-0.06796771287918091,
0.025509927421808243,
0.08646444976329803,
-0.026125987991690636,
-0.08090278506278992,
-0.010556885972619057,
-0.014372791163623333,
-0.040426257997751236,
-0.02022889442741871,
-0.08740727603435516,
-0.019767004996538162,
0.012218681164085865,
-0.0028640644159168005,
0.03715307638049126,
-0.0003772444906644523,
-0.006626375485211611,
0.034437231719493866,
-0.024719422683119774,
-0.0196191668510437,
0.070668064057827,
0.0418974794447422,
0.053349610418081284,
0.09215255826711655,
0.001424472313374281,
0.020453672856092453,
0.07670892030000687,
0.0355086624622345,
-0.0024904222227633,
-0.029500525444746017,
-0.09645874053239822,
-0.003191982163116336,
-0.022090476006269455,
-0.021126480773091316,
0.04619830101728439,
0.0013814133126288652,
-0.016334403306245804,
-0.009650482796132565,
0.01892758533358574,
-0.010515807196497917,
0.07107781618833542,
0.047808632254600525,
0.04314915090799332,
-0.03371540084481239,
-0.005788284353911877,
-0.06277381628751755,
-0.003936991561204195,
0.01095203123986721,
-0.26651692390441895,
-0.011887019500136375,
0.007848454639315605,
0.03238949552178383,
0.10760802775621414,
-0.0058823986910283566,
0.04653334617614746,
0.00836848746985197,
-0.04318952187895775,
-0.005027003586292267,
0.03763921558856964,
0.03788717836141586,
0.04821848124265671,
0.03395789489150047,
-0.028599588200449944,
-0.01928265020251274,
0.014585399068892002,
0.002672066679224372,
-0.07390908151865005,
0.045752108097076416,
-0.024666083976626396,
-0.0507764108479023,
0.0920770987868309,
0.04734477400779724,
-0.03257349506020546,
0.05372823402285576,
-0.05788154527544975,
-0.017247118055820465,
0.0087873050943017,
0.028999999165534973,
-0.005810897797346115,
-0.003708628239110112,
0.11785975843667984,
-0.1008489802479744,
0.019002282992005348,
0.04952186718583107,
-0.04641946777701378,
0.006521387491375208,
-0.021293796598911285,
0.04945763200521469,
-0.030439354479312897,
-0.0033646526280790567,
-0.029997631907463074,
-0.007164893671870232,
0.092595674097538,
-0.04479113966226578,
-0.0647326111793518,
-0.0546034500002861,
0.031431809067726135,
0.03413093835115433,
-0.03482935577630997,
0.01554905530065298,
-0.03393147885799408,
0.048407647758722305,
0.04785153642296791,
0.037702616304159164,
0.059705302119255066,
-0.016118114814162254,
-0.016264120116829872,
0.005214792676270008,
-0.015147598460316658,
-0.002484395867213607,
-0.026740768924355507,
0.06482615321874619,
0.060136716812849045
] |
PMID:8292 | 3-(Hydroxymethyl)-8-methoxychromone and unconjugated metabolites in rat plasma. Identification of the biologically active species. | After administering 14C-labeled 3-(hydroxymethyl)-8-methoxychromone to rats by gavage, plasma was found to contain unchanged compound and three unconjugated metabolites. These metabolites were identified as 3-carboxy-8-methoxychromone, 8-methoxychromone, and 2-hydroxy-3-methoxyactophenone. The plasma levels of all four labeled compounds were determined from 30 min to 48 hr after drug administration. Only the levels of 3-(hydroxymethyl)-8-methoxychromone and 3-carboxy-8-methoxychromone correlated with the expression of antiallergy activity in the rat, and only these compounds were found to be active in vivo. However, a test system for the inhibition of anaphylactic histamine release in vitro showed activity only for 3-carboxy-8-methoxychromone. | 3-(Hydroxymethyl)-8-methoxychromone and unconjugated metabolites in rat plasma. Identification of the biologically active species. After administering 14C-labeled 3-(hydroxymethyl)-8-methoxychromone to rats by gavage, plasma was found to contain unchanged compound and three unconjugated metabolites. These metabolites were identified as 3-carboxy-8-methoxychromone, 8-methoxychromone, and 2-hydroxy-3-methoxyactophenone. The plasma levels of all four labeled compounds were determined from 30 min to 48 hr after drug administration. Only the levels of 3-(hydroxymethyl)-8-methoxychromone and 3-carboxy-8-methoxychromone correlated with the expression of antiallergy activity in the rat, and only these compounds were found to be active in vivo. However, a test system for the inhibition of anaphylactic histamine release in vitro showed activity only for 3-carboxy-8-methoxychromone. | [
-0.02627447433769703,
-0.029049750417470932,
-0.010911799967288971,
0.05392635241150856,
0.04919815808534622,
0.00888215284794569,
0.05511859059333801,
-0.03105340711772442,
-0.07720862329006195,
-0.009175319224596024,
0.016884665936231613,
-0.026093797758221626,
-0.033681027591228485,
0.010106261819601059,
-0.011271235533058643,
0.019859671592712402,
0.011506495997309685,
0.0022097062319517136,
-0.07047299295663834,
0.02325223572552204,
-0.03219640254974365,
0.053408313542604446,
0.06496649235486984,
0.05264566093683243,
0.0770745649933815,
0.06454335153102875,
-0.0064679160714149475,
0.018336227163672447,
-0.07134781777858734,
-0.19482751190662384,
0.0036625934299081564,
0.027424881234765053,
0.023559564724564552,
-0.006013117730617523,
-0.007409938145428896,
0.006328490097075701,
-0.03249673545360565,
0.003915769979357719,
0.03690052404999733,
0.056952040642499924,
0.0001690146600594744,
-0.01493053324520588,
-0.015465873293578625,
0.057654496282339096,
-0.01298094168305397,
0.07778973132371902,
-0.011723506264388561,
0.019022703170776367,
0.08476434648036957,
-0.083206906914711,
0.026113085448741913,
0.05864891782402992,
-0.005761902313679457,
0.05818073824048042,
-0.02220357023179531,
0.010006827302277088,
-0.007626981940120459,
0.001775333657860756,
-0.021442314609885216,
0.10036192834377289,
-0.01685038022696972,
0.07835734635591507,
-0.14529383182525635,
0.04544796049594879,
0.04100436717271805,
0.010146704502403736,
-0.00496290298178792,
-0.049386896193027496,
0.04940133914351463,
0.09270711243152618,
-0.050981875509023666,
0.010862164199352264,
-0.03952164202928543,
0.023754794150590897,
0.050862867385149,
0.024697961285710335,
-0.018902292475104332,
-0.03191198781132698,
0.0004766299098264426,
-0.013686629012227058,
-0.03375644236803055,
0.01795334368944168,
-0.019168145954608917,
-0.019118737429380417,
0.05056188255548477,
-0.05354522913694382,
0.029343225061893463,
-0.023236270993947983,
0.007125235162675381,
0.01566382683813572,
-0.032213978469371796,
-0.046882178634405136,
-0.050070591270923615,
-0.017418889328837395,
-0.06934934854507446,
-0.04285421222448349,
0.03422166407108307,
0.007008473854511976,
-0.008360173553228378,
0.3288024365901947,
-0.030358025804162025,
0.0085246367380023,
-0.07065767794847488,
-0.021776115521788597,
-0.053040869534015656,
0.031351346522569656,
-0.06606573611497879,
0.0061563970521092415,
-0.035285379737615585,
0.02440519817173481,
0.05463032051920891,
0.028997644782066345,
0.014044824987649918,
-0.09864623099565506,
-0.03218673914670944,
0.0037359227426350117,
0.010488365776836872,
-0.025420887395739555,
0.005747919902205467,
0.014236090704798698,
0.10771363228559494,
-0.014150721952319145,
0.03219309449195862,
-0.026906555518507957,
0.02429831214249134,
0.06592486053705215,
0.01036093384027481,
0.07298074662685394,
0.02722538635134697,
0.0038900948129594326,
0.00589522672817111,
-0.018832210451364517,
-0.0019076371099799871,
0.025917282328009605,
-0.00497929472476244,
-0.03521124646067619,
0.08742249757051468,
0.002049435628578067,
0.004956461489200592,
0.0019812637474387884,
-0.025857865810394287,
-0.0715005099773407,
-0.030727457255125046,
-0.09198641031980515,
-0.10305082052946091,
0.1310417205095291,
0.018692228943109512,
0.02209290862083435,
-0.002083010273054242,
-0.008129958063364029,
0.03996475040912628,
0.07219725847244263,
-0.08667812496423721,
0.024662384763360023,
-0.04222923889756203,
0.040450792759656906,
-0.02129191718995571,
0.014824286103248596,
-0.030838198959827423,
0.0440523661673069,
-0.12078161537647247,
-0.018966233357787132,
0.011003680527210236,
0.08005416393280029,
-0.00936280656605959,
-0.047243382781744,
-0.006258257199078798,
0.062004368752241135,
0.022552739828824997,
0.007721283473074436,
-0.008196170441806316,
0.013476123102009296,
-0.04576651751995087,
0.024642884731292725,
-0.04468166455626488,
-0.04800761491060257,
0.048146896064281464,
0.005483278539031744,
0.0009269989677704871,
-0.011058219708502293,
0.03604111447930336,
-0.04182283207774162,
-0.02424883469939232,
0.02746664360165596,
-0.009969937615096569,
0.0025528157129883766,
-0.018621129915118217,
0.012172733433544636,
-0.011313050054013729,
0.07164927572011948,
-0.01570810377597809,
0.008966883644461632,
-0.013514883816242218,
0.01588703878223896,
-0.026582954451441765,
-0.01763826794922352,
-0.07448090612888336,
0.038246650248765945,
0.046352922916412354,
0.008100886829197407,
0.11108248680830002,
-0.005311110522598028,
0.059428587555885315,
0.04738297685980797,
0.040676187723875046,
0.053222883492708206,
-0.0037277634255588055,
-0.012830100022256374,
0.03359205648303032,
-0.01610437221825123,
-0.012441149912774563,
0.04243161156773567,
-0.02908286824822426,
0.05113830789923668,
-0.0033953008241951466,
-0.01512495893985033,
-0.03281635418534279,
-0.02811957709491253,
0.009682628326117992,
0.033404771238565445,
-0.003564084181562066,
-0.07350261509418488,
-0.034458890557289124,
-0.30448630452156067,
-0.05710583180189133,
0.0363108329474926,
-0.023648792877793312,
0.013216683641076088,
-0.014299938455224037,
-0.009069827385246754,
-0.016075558960437775,
-0.016745707020163536,
-0.051341187208890915,
0.03021685965359211,
0.042840734124183655,
-0.012333426624536514,
-0.07799842208623886,
0.004902776330709457,
0.038291189819574356,
0.04099586606025696,
-0.009581792168319225,
0.0680709108710289,
0.0005991000216454268,
0.015277376398444176,
-0.002482295036315918,
-0.025076674297451973,
-0.008874346502125263,
0.07108358293771744,
0.006619414314627647,
0.09168435633182526,
0.0006611375138163567,
-0.08962153643369675,
-0.028517095372080803,
0.05800499767065048,
0.011203818954527378,
-0.006357732694596052,
-0.04399389773607254,
0.009614787995815277,
-0.06639024615287781,
-0.03849927335977554,
-0.019855506718158722,
0.014498928561806679,
0.0015324645210057497,
-0.0731784775853157,
-0.017712946981191635,
0.019204413518309593,
-0.10353819280862808,
-0.020753102377057076,
-0.013260744512081146,
0.02685137651860714,
-0.026079611852765083,
-0.006627082359045744,
-0.08051623404026031,
0.008705348707735538,
0.053598225116729736,
0.03193877637386322,
-0.024999532848596573,
0.004055322147905827,
0.02334466576576233,
-0.09097221493721008,
-0.043308038264513016,
-0.06490675359964371,
0.036391954869031906,
0.022373907268047333,
0.02468082867562771,
-0.021610988304018974,
-0.022260848432779312,
0.028240136802196503,
0.011136643588542938,
-0.018154093995690346,
-0.0888599082827568,
0.06494038552045822,
0.013785447925329208,
0.0531863309442997,
0.12659147381782532,
-0.02594229020178318,
0.004763630218803883,
0.04336908087134361,
-0.004608894232660532,
-0.03789152577519417,
-0.0316920205950737,
-0.0903535708785057,
0.06631673872470856,
-0.0021239311899989843,
-0.018854472786188126,
0.025598451495170593,
0.002516542561352253,
0.01416061446070671,
0.011705267243087292,
-0.019974324852228165,
0.0022677273955196142,
0.0388697125017643,
0.009507175534963608,
-0.0034605839755386114,
0.038422614336013794,
0.027766847983002663,
-0.06000550091266632,
0.07667779177427292,
0.012555735185742378,
-0.2572907507419586,
-0.013396536000072956,
0.05669813230633736,
0.022887226194143295,
0.03176329657435417,
-0.0616808757185936,
0.03205617144703865,
-0.059313613921403885,
-0.0441216379404068,
-0.013527007773518562,
0.031860630959272385,
0.04732530191540718,
0.039585866034030914,
0.06281477957963943,
-0.0014460175298154354,
-0.021730508655309677,
0.039740998297929764,
0.037345051765441895,
-0.01382001768797636,
-0.0059831044636666775,
0.010555780492722988,
-0.07806192338466644,
0.10159717500209808,
0.02018362283706665,
-0.05644908919930458,
0.03940353915095329,
0.02311987616121769,
0.05075167864561081,
-0.04914813116192818,
0.008376584388315678,
-0.01770768128335476,
0.01725948601961136,
0.09555673599243164,
-0.044981520622968674,
0.008616209961473942,
-0.010767700150609016,
-0.028471820056438446,
-0.013812173157930374,
-0.016721239313483238,
0.03588712587952614,
-0.012114152312278748,
0.030185619369149208,
-0.04244969040155411,
-0.02695450745522976,
0.03970269113779068,
-0.06107307970523834,
-0.022081060335040092,
-0.030672186985611916,
0.014831854030489922,
-0.01605326682329178,
-0.022956741973757744,
0.011469732038676739,
-0.024188384413719177,
0.05996944010257721,
-0.016881868243217468,
0.02413366734981537,
0.030019832774996758,
-0.019882526248693466,
-0.029089393094182014,
-0.04214351251721382,
-0.018480224534869194,
0.0037738806568086147,
-0.08184976875782013,
0.09038425981998444,
0.05917701497673988
] |
PMID:8293 | Metabolism of tripelennamine in man. | Four polar metabolites were isolated from the urine of human subjects orally treated with tripelennamine, and their structures elucidated by various chemical and physical methods. One of the metabolites, which is a minor one, was identified as an N-oxide of tripelennamine, and the other three as glucuronide conjugates. One of the conjugates, which is a major metabolite, has been assigned a unique quaternary ammonium N-glucuronide structure, since it gave tripelennamine and D-glucuronic acid on incubation with beta-glucuronidase. The N-oxide, which has also been prepared synthetically, remained unchanged on similar treatment. The other two conjugates were O-glucuronides of hydroxylated derivatives, the glucuronide of hydroxytripelennamine being the principal metabolite. No desmethyltripelennamine was found in the urine, however. Hydroxylation in both cases had occurred in the pyridine ring. | Metabolism of tripelennamine in man. Four polar metabolites were isolated from the urine of human subjects orally treated with tripelennamine, and their structures elucidated by various chemical and physical methods. One of the metabolites, which is a minor one, was identified as an N-oxide of tripelennamine, and the other three as glucuronide conjugates. One of the conjugates, which is a major metabolite, has been assigned a unique quaternary ammonium N-glucuronide structure, since it gave tripelennamine and D-glucuronic acid on incubation with beta-glucuronidase. The N-oxide, which has also been prepared synthetically, remained unchanged on similar treatment. The other two conjugates were O-glucuronides of hydroxylated derivatives, the glucuronide of hydroxytripelennamine being the principal metabolite. No desmethyltripelennamine was found in the urine, however. Hydroxylation in both cases had occurred in the pyridine ring. | [
-0.03587665408849716,
-0.024273741990327835,
-0.007142307702451944,
0.04466806352138519,
0.018745116889476776,
-0.004227860365062952,
0.040681175887584686,
-0.03127714991569519,
-0.041236162185668945,
-0.01719333603978157,
0.02161838859319687,
-0.007872291840612888,
0.014200881123542786,
-0.0042428686283528805,
0.005767934024333954,
-0.04363846778869629,
0.00827520526945591,
-0.03934647515416145,
0.014358635991811752,
0.006505103316158056,
0.043135613203048706,
0.0009562238701619208,
0.011658193543553352,
0.04303494095802307,
0.047795459628105164,
0.03918018937110901,
0.05699856951832771,
0.026070021092891693,
-0.05160020664334297,
-0.19321033358573914,
0.011524764820933342,
0.0656244233250618,
-0.034046612679958344,
-0.036490485072135925,
-0.06330917775630951,
0.03473752737045288,
-0.03897703438997269,
0.06917394697666168,
0.047608632594347,
0.030887041240930557,
0.0040260241366922855,
0.022510439157485962,
0.019724609330296516,
0.0011420662049204111,
0.0181927178055048,
0.09063330292701721,
-0.07575022429227829,
-0.026836810633540154,
0.12228147685527802,
0.028072113171219826,
0.022644512355327606,
0.027529992163181305,
0.029747525230050087,
0.059223566204309464,
-0.0042629423551261425,
0.000050432099669706076,
0.00930526852607727,
-0.030871732160449028,
-0.015626700595021248,
0.04219837486743927,
-0.03293516859412193,
0.068728007376194,
-0.11922815442085266,
0.0396832637488842,
0.0019570428412407637,
-0.0034816658589988947,
-0.02556770108640194,
-0.020469846203923225,
0.033777132630348206,
0.08762405067682266,
-0.03374017775058746,
-0.020292524248361588,
-0.03828364610671997,
-0.040737394243478775,
-0.015308373607695103,
0.017612790688872337,
0.010370434261858463,
-0.0029980659019201994,
-0.004430876579135656,
-0.01297249086201191,
0.04166359081864357,
0.027676619589328766,
0.06614471971988678,
0.016125846654176712,
0.04230738431215286,
-0.024101458489894867,
0.03787258267402649,
0.00020397714979480952,
0.010715474374592304,
-0.016680698841810226,
-0.003100767033174634,
-0.04645627737045288,
-0.02865707315504551,
-0.026663200929760933,
-0.07583317905664444,
-0.002826225245371461,
0.023810196667909622,
0.029139496386051178,
0.011060775257647038,
0.32732638716697693,
-0.02854551561176777,
0.003708063857629895,
-0.04032699391245842,
0.02104494720697403,
-0.04920000582933426,
0.012201819568872452,
-0.01490061916410923,
-0.017074493691325188,
0.041922204196453094,
0.04541166126728058,
0.06054854020476341,
0.04908696934580803,
0.01580137573182583,
-0.0997578576207161,
-0.07164370268583298,
-0.012264533899724483,
0.01927780732512474,
-0.015281575731933117,
-0.04940592870116234,
0.02560703456401825,
0.03518544137477875,
-0.020230736583471298,
0.02919844724237919,
-0.006823738105595112,
0.01767539791762829,
0.03610081970691681,
-0.04258322715759277,
0.07438307255506516,
0.042672231793403625,
0.039893653243780136,
0.005137643311172724,
-0.00522388843819499,
0.03831517696380615,
-0.034462448209524155,
-0.026684030890464783,
0.046282436698675156,
0.09575600177049637,
-0.038927122950553894,
-0.02110111340880394,
0.05216393992304802,
-0.04449428617954254,
-0.045044656842947006,
-0.07808059453964233,
-0.03288901969790459,
-0.0834471732378006,
0.10033565014600754,
0.00882258266210556,
-0.004406601656228304,
0.009381825104355812,
0.049528393894433975,
0.02613295242190361,
0.0697847306728363,
-0.07394473254680634,
-0.00877976231276989,
-0.015828581526875496,
-0.02887647971510887,
-0.03724610432982445,
0.005151183810085058,
-0.013005628250539303,
0.0368475578725338,
-0.08511501550674438,
0.011657075956463814,
0.0002851076715160161,
0.12725991010665894,
0.00454844580963254,
-0.06638899445533752,
-0.07477511465549469,
0.0332040973007679,
0.0008328589610755444,
-0.011695708148181438,
0.03452589362859726,
-0.049926310777664185,
-0.03475125506520271,
0.0307045616209507,
-0.022241270169615746,
-0.02021992765367031,
0.040093421936035156,
0.024248715490102768,
-0.04071033000946045,
0.03402036800980568,
0.10208126902580261,
-0.04523174837231636,
-0.03958168253302574,
-0.03944474086165428,
0.0795237123966217,
-0.034147199243307114,
-0.010066414251923561,
0.0062998938374221325,
0.0009937400463968515,
0.03981070965528488,
-0.08730316907167435,
-0.027287589386105537,
-0.05076045170426369,
0.03245143964886665,
-0.04047060385346413,
-0.010474461130797863,
-0.12055524438619614,
0.023778701201081276,
0.004382419399917126,
-0.015912752598524094,
0.07792937010526657,
0.03890807554125786,
0.008379824459552765,
0.05225710570812225,
0.03844635933637619,
0.02097536250948906,
-0.004507844336330891,
0.015616628341376781,
0.0006037757848389447,
0.016223818063735962,
-0.009240962564945221,
0.006664526183158159,
-0.029949650168418884,
0.042410362511873245,
-0.020020892843604088,
0.0072610643692314625,
0.032592110335826874,
-0.04985677823424339,
0.029234908521175385,
0.07407437264919281,
0.025780074298381805,
-0.12158814072608948,
-0.08212970197200775,
-0.31510114669799805,
-0.0917905867099762,
0.0359991230070591,
-0.01604164019227028,
0.023875147104263306,
-0.05663727968931198,
-0.02803385816514492,
0.0047261472791433334,
0.035467371344566345,
-0.029849138110876083,
0.029690120369195938,
0.0586366280913353,
-0.009734809398651123,
-0.08350484818220139,
-0.027940472587943077,
0.03262332081794739,
0.05249129608273506,
-0.02015586942434311,
0.043365687131881714,
-0.019278423860669136,
0.014437534846365452,
-0.032727453857660294,
0.03509671986103058,
0.027983827516436577,
0.06319356709718704,
-0.0384887233376503,
0.14114059507846832,
0.004010302480310202,
-0.03194273263216019,
-0.020005246624350548,
0.04141001030802727,
0.02266174741089344,
-0.039100077003240585,
-0.055543944239616394,
0.02931802347302437,
-0.07439345121383667,
-0.04009787365794182,
-0.06281128525733948,
0.004074081778526306,
-0.06302100419998169,
-0.027636107057332993,
0.017065085470676422,
0.05474117025732994,
-0.0805969312787056,
-0.013591231778264046,
-0.021461904048919678,
-0.03912743553519249,
-0.05062016472220421,
-0.00521118426695466,
-0.02807476371526718,
0.023265453055500984,
0.03662358969449997,
0.01847345009446144,
-0.04510588198900223,
-0.009028458036482334,
-0.016073914244771004,
-0.11011970043182373,
-0.049232061952352524,
-0.04252716898918152,
0.007761996239423752,
-0.016687415540218353,
0.020524028688669205,
0.011851292103528976,
-0.02658294141292572,
0.01900065504014492,
-0.0079932427033782,
0.010687429457902908,
-0.0429515466094017,
0.07760599255561829,
0.022583505138754845,
0.050082046538591385,
0.12139326333999634,
-0.03366662189364433,
0.05387374013662338,
0.015734879299998283,
0.0034746488090604544,
-0.011967190541327,
-0.011022690683603287,
-0.06737403571605682,
0.03227776288986206,
-0.055954791605472565,
-0.017535926774144173,
0.046658340841531754,
0.062206707894802094,
-0.026532327756285667,
0.007722752634435892,
0.010244417004287243,
-0.05805600807070732,
0.02782800793647766,
0.03642534837126732,
0.04763912409543991,
0.020621981471776962,
-0.00736917182803154,
-0.06315954029560089,
-0.0019422100158408284,
0.04764575883746147,
-0.20966631174087524,
-0.0022368705831468105,
0.031546466052532196,
0.016576003283262253,
0.0663670003414154,
0.011747563257813454,
0.033750370144844055,
-0.005412410944700241,
-0.034969840198755264,
0.05624282732605934,
0.0597531832754612,
0.034296914935112,
0.0770290344953537,
-0.0031713219359517097,
0.028988352045416832,
0.05905769392848015,
0.09167472273111343,
0.023760199546813965,
-0.03524218127131462,
-0.006051893346011639,
-0.010222858749330044,
0.0135331517085433,
0.10996847599744797,
0.006219870410859585,
-0.023740582168102264,
0.0466795489192009,
-0.022275477647781372,
0.031050637364387512,
-0.04332489147782326,
0.004297355655580759,
-0.041998833417892456,
0.0005386723205447197,
0.07328186929225922,
-0.057929396629333496,
0.04262373968958855,
0.01662258617579937,
-0.016374114900827408,
-0.0029055022168904543,
-0.00600907439365983,
0.012402270920574665,
-0.06785967200994492,
0.017134083434939384,
-0.08186893165111542,
-0.01274486631155014,
0.05695284903049469,
-0.05588124692440033,
-0.0634031817317009,
-0.06870028376579285,
0.019806865602731705,
-0.027724750339984894,
0.0033183395862579346,
-0.0288170725107193,
-0.033109940588474274,
0.05506410449743271,
0.04392251744866371,
-0.018781300634145737,
-0.04114634916186333,
-0.01558434683829546,
-0.009032170288264751,
-0.022173888981342316,
-0.054980915039777756,
0.017892980948090553,
-0.014931462705135345,
0.024503840133547783,
0.028273947536945343
] |
PMID:8294 | Disposition and metabolism of 3-(3-chlorophenoxy)-N-methylpyrrolidine [14C]-carboxamide in the rat, dog, and man. | Studies with a 14C-labeled sample have shown that the title compound A is readily absorbed and very rapidly excreted by the rat, dog, and man. The metabolites result from aromatic ring hydroxylation, oxidation, and dealkylation of the urea methyl group, and oxidative ring opening of the pyrrolidine ring. Rat metabolites were 3-(3-chloro-4-hydroxyphenoxy)-N-methyl-l-pyrrolidinecarboxamide (C) 3-(3-chloro-4-hydroxyphenoxy)-1-pyrrolidinecarboxamide (D), 3-(3-chlorohydroxyphenoxy)-1-pyrrolidinecarboxamide (E), 3-(3-chlorophenoxy)-4([(methylamino)-carbonyl]amino)butanoic acid (G), 3-(3-chlorophenoxy)-4[(aminocarbonyl)amino]butanoic acid (H). Dog metabolites were C, D, G, and H. Human metabolites were 3-(3-chloro-4-hydroxyphenoxy)-N-formyl-l-pyrrolidinecarboxamide (F), C, D, G, and H. An electron-capture gas chromatographic assay for the parent compound is described. Whole-body autoradiograms of rat slices and the tissue residue data from these slices are reported and indicate rapid tissue depletion of radioactivity. | Disposition and metabolism of 3-(3-chlorophenoxy)-N-methylpyrrolidine [14C]-carboxamide in the rat, dog, and man. Studies with a 14C-labeled sample have shown that the title compound A is readily absorbed and very rapidly excreted by the rat, dog, and man. The metabolites result from aromatic ring hydroxylation, oxidation, and dealkylation of the urea methyl group, and oxidative ring opening of the pyrrolidine ring. Rat metabolites were 3-(3-chloro-4-hydroxyphenoxy)-N-methyl-l-pyrrolidinecarboxamide (C) 3-(3-chloro-4-hydroxyphenoxy)-1-pyrrolidinecarboxamide (D), 3-(3-chlorohydroxyphenoxy)-1-pyrrolidinecarboxamide (E), 3-(3-chlorophenoxy)-4([(methylamino)-carbonyl]amino)butanoic acid (G), 3-(3-chlorophenoxy)-4[(aminocarbonyl)amino]butanoic acid (H). Dog metabolites were C, D, G, and H. Human metabolites were 3-(3-chloro-4-hydroxyphenoxy)-N-formyl-l-pyrrolidinecarboxamide (F), C, D, G, and H. An electron-capture gas chromatographic assay for the parent compound is described. Whole-body autoradiograms of rat slices and the tissue residue data from these slices are reported and indicate rapid tissue depletion of radioactivity. | [
-0.06227082386612892,
0.010334879159927368,
0.013620557263493538,
-0.0009398978436365724,
0.016941744834184647,
0.012526345439255238,
0.037296853959560394,
0.0030889268964529037,
-0.07223541289567947,
-0.007026101928204298,
0.03589952737092972,
-0.043359920382499695,
0.002825481351464987,
0.018808992579579353,
0.009969634935259819,
-0.01743350178003311,
-0.037031374871730804,
-0.021985042840242386,
-0.05219850689172745,
-0.018215127289295197,
0.009893815033137798,
-0.032469868659973145,
0.03587767854332924,
0.015342150814831257,
0.06699536740779877,
0.05938373878598213,
0.013617350719869137,
-0.0016503924271091819,
-0.06246783956885338,
-0.19822268187999725,
-0.032994259148836136,
0.07912890613079071,
0.01841406710445881,
-0.0699126198887825,
-0.026802122592926025,
0.010973883792757988,
0.02377314493060112,
0.028103072196245193,
0.02604386955499649,
0.0409960001707077,
0.022138044238090515,
0.006654808763414621,
0.011648925952613354,
0.010130157694220543,
0.0023986813612282276,
0.054985884577035904,
-0.0579497404396534,
0.019835157319903374,
0.10584532469511032,
-0.008501596748828888,
-0.016108594834804535,
0.03261561319231987,
0.014170759357511997,
0.05951247364282608,
0.0020062876865267754,
-0.02883375808596611,
0.042380038648843765,
-0.009407026693224907,
-0.022494511678814888,
0.02816888876259327,
-0.0035080628003925085,
0.0693298876285553,
-0.1688852310180664,
0.03798648342490196,
0.030892264097929,
0.012753291055560112,
-0.044687192887067795,
-0.015516262501478195,
0.0369340144097805,
0.05706961825489998,
-0.051025692373514175,
-0.0007687659235671163,
-0.04503605142235756,
0.015192151069641113,
0.005020345561206341,
-0.016613826155662537,
-0.01761552132666111,
-0.04534747824072838,
0.01236738171428442,
-0.028804372996091843,
-0.012995724566280842,
0.021167654544115067,
-0.0046831355430185795,
-0.04684850946068764,
0.041232652962207794,
-0.019195685163140297,
0.006069471128284931,
-0.031698793172836304,
-0.024188430979847908,
0.01879451423883438,
-0.004544385708868504,
-0.1260603368282318,
-0.03137447312474251,
-0.024373024702072144,
-0.08341094851493835,
-0.024719467386603355,
0.062270209193229675,
-0.0023261173628270626,
0.0018824146827682853,
0.33807823061943054,
0.004154754802584648,
0.014831732027232647,
-0.06131356954574585,
0.015563982538878918,
-0.017432937398552895,
-0.005218232981860638,
0.009153677150607109,
0.015784546732902527,
0.04188524931669235,
0.02824944630265236,
0.0904807299375534,
0.035534635186195374,
0.02261623926460743,
-0.07932028919458389,
-0.03731194883584976,
0.036613576114177704,
0.030350545421242714,
-0.0061700609512627125,
-0.01234851498156786,
0.029436388984322548,
0.026117099449038506,
-0.019718775525689125,
0.07657330483198166,
0.032987646758556366,
0.014097019098699093,
0.04271484911441803,
0.013223185203969479,
0.07439520955085754,
0.0856938585639,
0.058095697313547134,
0.03670630231499672,
0.03478162735700607,
0.0007925838581286371,
0.016133606433868408,
-0.017621800303459167,
-0.011272264644503593,
0.02554807811975479,
-0.039572425186634064,
-0.01820048876106739,
0.017575684934854507,
-0.009336745366454124,
-0.06652594357728958,
-0.045551687479019165,
-0.08801040798425674,
-0.09731525182723999,
0.08670244365930557,
0.015622829087078571,
-0.03782161325216293,
-0.05255429446697235,
0.008711996488273144,
0.02001773752272129,
0.08513905107975006,
0.013757871463894844,
-0.0003087559016421437,
-0.04628531634807587,
0.027460699900984764,
-0.030707266181707382,
-0.021693816408514977,
0.004703553859144449,
0.012351825833320618,
-0.10543978959321976,
-0.0651509091258049,
-0.021974792703986168,
0.11916954815387726,
-0.00440462538972497,
-0.012282559648156166,
-0.016691846773028374,
0.04667381942272186,
0.024247465655207634,
0.05205888673663139,
0.015625908970832825,
0.013587762601673603,
-0.04835290461778641,
0.022727608680725098,
-0.07905049622058868,
0.0005552114453166723,
0.05060979351401329,
0.04507528245449066,
0.01828395016491413,
-0.014646843075752258,
0.04844008386135101,
-0.013640469871461391,
-0.027429189532995224,
-0.0000945711144595407,
0.008543139323592186,
0.005367293022572994,
0.011513819918036461,
0.03516822308301926,
-0.00022303358127828687,
0.010274957865476608,
-0.02646794356405735,
-0.003941645845770836,
-0.013646841049194336,
0.008257079869508743,
-0.08353836834430695,
0.014015472494065762,
-0.07354912161827087,
0.04072073847055435,
0.012249749153852463,
-0.04411056637763977,
0.08118097484111786,
0.02367655746638775,
0.008785680867731571,
0.013311113230884075,
0.061111997812986374,
0.023190051317214966,
-0.016628015786409378,
-0.002558527048677206,
0.033649101853370667,
-0.04063135385513306,
-0.012438652105629444,
0.005116242915391922,
-0.008561943657696247,
0.049101322889328,
0.007197943516075611,
0.0873882994055748,
0.024612708017230034,
-0.015406828373670578,
0.017924489453434944,
0.05275823548436165,
0.03077901341021061,
-0.09108929336071014,
-0.04430623725056648,
-0.27816280722618103,
-0.04331961274147034,
-0.008323346264660358,
-0.04172131419181824,
0.039332810789346695,
-0.015265459194779396,
-0.027638906612992287,
-0.026542993262410164,
0.02361604943871498,
-0.032113879919052124,
0.06979069113731384,
0.04067208990454674,
-0.06329488754272461,
-0.040750522166490555,
0.03643728792667389,
0.03217407315969467,
0.0470389723777771,
-0.03760959580540657,
-0.006488498765975237,
-0.018963219597935677,
-0.01296569500118494,
-0.05210784822702408,
-0.0452287457883358,
0.009997962042689323,
0.06976692378520966,
-0.026561250910162926,
0.1286180019378662,
-0.037367552518844604,
0.006325217429548502,
-0.054529737681150436,
-0.00417425949126482,
0.04112008586525917,
-0.058500055223703384,
-0.07374819368124008,
-0.014185067266225815,
-0.04842623323202133,
0.003721546847373247,
-0.03796280920505524,
-0.032990310341119766,
-0.004415941424667835,
-0.05240415409207344,
-0.010077276267111301,
0.012519404292106628,
-0.11598330736160278,
-0.06465229392051697,
-0.006324528716504574,
-0.0680881142616272,
0.01379393134266138,
0.007909764535725117,
-0.013422857969999313,
0.04893878847360611,
0.016604218631982803,
-0.04060960188508034,
-0.025459859520196915,
-0.028197316452860832,
-0.015488501638174057,
-0.04752248898148537,
-0.015115492977201939,
-0.045227982103824615,
0.011403655633330345,
-0.004959987476468086,
-0.025586379691958427,
0.03802298381924629,
-0.043676622211933136,
-0.015858270227909088,
-0.006273115985095501,
0.023785870522260666,
-0.003273040521889925,
0.047956593334674835,
0.04059663042426109,
0.03584546595811844,
0.10386770963668823,
-0.05016438663005829,
0.05962375923991203,
-0.00290183094330132,
0.04073628410696983,
-0.007967531681060791,
-0.024490248411893845,
-0.03532542660832405,
0.06344643235206604,
0.011691928841173649,
0.06319861859083176,
0.03431910276412964,
0.0009291173773817718,
0.024099377915263176,
-0.025191569700837135,
-0.005866445600986481,
0.009484115056693554,
0.062166426330804825,
0.049282222986221313,
0.04397567734122276,
0.03677397593855858,
-0.031206604093313217,
-0.12182404100894928,
0.056196752935647964,
0.03318027779459953,
-0.27680936455726624,
-0.03240665793418884,
0.02758767455816269,
0.06115981563925743,
0.047065600752830505,
-0.009087218903005123,
0.010477535426616669,
-0.02012488804757595,
-0.04745209962129593,
-0.007611110806465149,
0.006574144121259451,
0.054873038083314896,
0.05145080387592316,
0.0632219985127449,
-0.013724970631301403,
-0.011048739776015282,
0.02672288753092289,
0.05770616605877876,
-0.0031177953351289034,
0.0008403667598031461,
-0.03616262599825859,
-0.08160937577486038,
0.11733680963516235,
0.037851545959711075,
-0.010730096139013767,
0.054261576384305954,
-0.0008516658563166857,
0.03283486142754555,
-0.012883925810456276,
0.008130368776619434,
-0.029932906851172447,
-0.0016507504042237997,
0.06343741714954376,
-0.050193075090646744,
0.03635622560977936,
-0.010473467409610748,
-0.004742394667118788,
0.021163158118724823,
-0.06444225460290909,
0.04365219175815582,
-0.04073045775294304,
0.04964036867022514,
-0.05711822584271431,
-0.014489361084997654,
0.055431947112083435,
-0.06729967892169952,
-0.022134408354759216,
-0.04809122532606125,
0.0729156956076622,
-0.006864066701382399,
0.011154145002365112,
0.03618019446730614,
-0.04916437715291977,
0.008501860313117504,
0.05304604768753052,
-0.042498599737882614,
0.02431456558406353,
-0.04133951663970947,
0.00214035133831203,
-0.055023133754730225,
-0.052053987979888916,
0.00325951911509037,
-0.03343549370765686,
0.08620628714561462,
0.07270785421133041
] |
PMID:8295 | Physiological disposition and metabolic fate of a new antiarrhythmic agent, alpha, alpha-dimethyl-4-(alpha, alpha, beta, beta-tetrafluorophenethyl) benzylamine in the rat, dog, monkey, baboon, and man. | The physiological disposition of a new orally active antiarrhythmic drug, alpha, alpha-dimethyl-4-(alpha, alpha, beta, beta-tetrafluorophenethyl)benzylamine (MK-251) was investigated in the rat, dog, rhesus monkey, baboon, and man. MK-251 was extensively absorbed after oral administration in all species. Fecal excretion was the major route of tracer elimination in the rat (70%) and dog (80%), whereas the monkey, baboon, and man excreted the majority of the dose via the urine (40-80%). MK-251 and/or its metabolites were widely distributed in rat tissues and exhibited tissue/plasma ratios greater than one in most instances. The lung, liver, and kidney possessed a high tissue affinity for drug and metabolites. The plasma and urinary profile of radioactivity indicated extensive metabolism of MK-251 in all species. Less than 5% of the plasma and urinary radioactivity was identified as unchanged drug. In spite of extensive metabolic transformations, a remarkable feature of this drug is its persistence in the plasma for long periods of time. This is thought to be due to tissue affinity. The metabolic pattern for MK-251 was essentially the same in all species. The major metabolites present in the plasma and the urine were identified as the carbinol analog of MK-251, 2-[4-(alpha, alpha, beta, beta-tetrafluorophenethyl)phenyl]-2-propanol (I), and its glucuronide conjugate. Other metabolites characterized in the urine and plasma were: the N-glucuronide of MK-251, 2-[4-(alpha, alpha, beta, beta-tetrafluorophenethyl)phenyl]propene (II), 2-nitro-2-[4-(alpha, alpha, beta, beta-tetrafluorophenethyl)phenyl]propane (III), alpha, alpha-dimethyl-4-(alpha, alpha, beta, beta-tetrafluorophenethyl)benzyl methyl ether (IV-1) and 4-(alpha, alpha, beta, beta-tetrafluorophenethyl), acetophenone (IV-2). Two minor urinary metabolites were tentatively identified as the N-hydroxy analog of MK-251 and the glycol analog of carbinol I. The in vivo formation of the methyl ether represents the first report of alkylation of a tertiary alcohol. | Physiological disposition and metabolic fate of a new antiarrhythmic agent, alpha, alpha-dimethyl-4-(alpha, alpha, beta, beta-tetrafluorophenethyl) benzylamine in the rat, dog, monkey, baboon, and man. The physiological disposition of a new orally active antiarrhythmic drug, alpha, alpha-dimethyl-4-(alpha, alpha, beta, beta-tetrafluorophenethyl)benzylamine (MK-251) was investigated in the rat, dog, rhesus monkey, baboon, and man. MK-251 was extensively absorbed after oral administration in all species. Fecal excretion was the major route of tracer elimination in the rat (70%) and dog (80%), whereas the monkey, baboon, and man excreted the majority of the dose via the urine (40-80%). MK-251 and/or its metabolites were widely distributed in rat tissues and exhibited tissue/plasma ratios greater than one in most instances. The lung, liver, and kidney possessed a high tissue affinity for drug and metabolites. The plasma and urinary profile of radioactivity indicated extensive metabolism of MK-251 in all species. Less than 5% of the plasma and urinary radioactivity was identified as unchanged drug. In spite of extensive metabolic transformations, a remarkable feature of this drug is its persistence in the plasma for long periods of time. This is thought to be due to tissue affinity. The metabolic pattern for MK-251 was essentially the same in all species. The major metabolites present in the plasma and the urine were identified as the carbinol analog of MK-251, 2-[4-(alpha, alpha, beta, beta-tetrafluorophenethyl)phenyl]-2-propanol (I), and its glucuronide conjugate. Other metabolites characterized in the urine and plasma were: the N-glucuronide of MK-251, 2-[4-(alpha, alpha, beta, beta-tetrafluorophenethyl)phenyl]propene (II), 2-nitro-2-[4-(alpha, alpha, beta, beta-tetrafluorophenethyl)phenyl]propane (III), alpha, alpha-dimethyl-4-(alpha, alpha, beta, beta-tetrafluorophenethyl)benzyl methyl ether (IV-1) and 4-(alpha, alpha, beta, beta-tetrafluorophenethyl), acetophenone (IV-2). Two minor urinary metabolites were tentatively identified as the N-hydroxy analog of MK-251 and the glycol analog of carbinol I. The in vivo formation of the methyl ether represents the first report of alkylation of a tertiary alcohol. | [
-0.045868169516325,
0.0031525942031294107,
-0.04563057795166969,
0.02881069853901863,
0.058605603873729706,
0.0009495364502072334,
0.0013997431378811598,
0.031993117183446884,
-0.06866147369146347,
-0.0459919199347496,
0.015529761090874672,
-0.0827275887131691,
-0.011937404982745647,
0.03466314822435379,
-0.05194499343633652,
0.011614756658673286,
0.03679698705673218,
-0.07200684398412704,
-0.028240418061614037,
-0.0028512366116046906,
0.02991919405758381,
-0.010061733424663544,
0.06681666523218155,
0.02936825528740883,
0.04411998763680458,
0.0506553128361702,
0.0019059792393818498,
-0.026840142905712128,
-0.042395297437906265,
-0.1911316066980362,
0.011588082648813725,
-0.007685039658099413,
-0.02878943830728531,
-0.047696225345134735,
-0.03789224103093147,
-0.012735622934997082,
-0.03136761486530304,
0.0510273277759552,
-0.0015801790868863463,
0.053311221301555634,
0.028140565380454063,
-0.0005710026598535478,
0.029925793409347534,
0.027538694441318512,
0.049844443798065186,
0.005762550514191389,
-0.0884171724319458,
0.0002341847139177844,
0.13271936774253845,
0.016155848279595375,
-0.006501545663923025,
-0.012801634147763252,
0.05073237419128418,
0.08503115922212601,
-0.004426486324518919,
-0.023014387115836143,
0.05766298621892929,
-0.007288409862667322,
-0.009161843918263912,
0.0875437781214714,
0.026416219770908356,
0.039309412240982056,
-0.16020555794239044,
0.026120897382497787,
0.037532396614551544,
0.022832196205854416,
-0.02731957845389843,
-0.011841281317174435,
0.027860084548592567,
0.06580569595098495,
-0.055657655000686646,
0.003673011204227805,
0.0003647673293016851,
-0.029720580205321312,
-0.016875235363841057,
-0.01686856709420681,
0.024426229298114777,
0.02357158623635769,
0.020994435995817184,
0.027131985872983932,
0.011343294754624367,
-0.008726323023438454,
-0.009578146040439606,
-0.06890140473842621,
0.005395693704485893,
-0.001251799170859158,
0.031064240261912346,
-0.032001785933971405,
0.0025687471497803926,
-0.03945823386311531,
-0.027263542637228966,
-0.03741014748811722,
-0.005573212169110775,
-0.013861275278031826,
-0.0788704976439476,
-0.00008875804633134976,
0.01368529163300991,
-0.0006592196878045797,
0.02757050283253193,
0.35171055793762207,
0.04805319011211395,
0.034037888050079346,
-0.11696221679449081,
0.01028797309845686,
-0.013954457826912403,
-0.01347969938069582,
0.023388907313346863,
0.03069300763309002,
0.037621915340423584,
-0.021168429404497147,
0.026285991072654724,
0.05567561835050583,
0.019712017849087715,
-0.03801916539669037,
-0.004677910823374987,
-0.00916624628007412,
0.03369540348649025,
0.0075090378522872925,
-0.006606645416468382,
0.019321519881486893,
0.009586188942193985,
-0.02979430742561817,
0.03854885697364807,
0.0007828061352483928,
0.06358085572719574,
0.005751494783908129,
-0.01764424704015255,
0.07254963368177414,
0.03763092681765556,
0.04306158423423767,
0.028504330664873123,
0.03639090433716774,
0.021779051050543785,
-0.016766034066677094,
-0.0066546788439154625,
-0.015452041290700436,
0.024985352531075478,
-0.0048872302286326885,
-0.04160924628376961,
0.03170783445239067,
-0.02252088487148285,
-0.035841505974531174,
-0.04118726775050163,
-0.09680511057376862,
-0.0915760025382042,
0.10576166957616806,
0.04416125267744064,
-0.03619644418358803,
-0.023717226460576057,
0.04116452485322952,
0.014651449397206306,
0.04203210771083832,
0.02015363611280918,
0.02475995384156704,
-0.036879222840070724,
0.06912299990653992,
-0.017776185646653175,
0.02229922078549862,
-0.06892473995685577,
0.014863039366900921,
-0.06493303179740906,
-0.03155149146914482,
0.002277693245559931,
0.11450348049402237,
0.004828702658414841,
0.010742999613285065,
-0.06403395533561707,
0.008882458321750164,
-0.008189615793526173,
0.01347570214420557,
0.025579437613487244,
-0.006010799668729305,
-0.007938006892800331,
0.031350597739219666,
-0.054960962384939194,
-0.014172988012433052,
0.02968311309814453,
-0.02363639697432518,
-0.02400222048163414,
0.007202002685517073,
0.033344995230436325,
0.024056773632764816,
-0.037725094705820084,
0.0032606013119220734,
0.036733850836753845,
0.04812014102935791,
-0.04857826232910156,
-0.014795850962400436,
0.02547260746359825,
0.037251222878694534,
-0.005987184122204781,
0.0053214444778859615,
-0.046903084963560104,
-0.008561807684600353,
-0.026095233857631683,
-0.03700505942106247,
-0.08647485077381134,
0.021833112463355064,
-0.024497859179973602,
-0.002377840457484126,
0.1144503727555275,
-0.006336362566798925,
0.017438946291804314,
0.05849093571305275,
0.07015135884284973,
0.033227622509002686,
-0.007362932898104191,
0.00476743932813406,
0.08045941591262817,
-0.013872643001377583,
-0.011203022673726082,
0.033420395106077194,
-0.0002318179904250428,
0.0041696298867464066,
-0.05058988556265831,
0.08412668853998184,
-0.012853339314460754,
0.010316690430045128,
0.044911906123161316,
0.014462443999946117,
0.0025397890713065863,
-0.07324501127004623,
-0.018802672624588013,
-0.3188411295413971,
-0.04237455874681473,
0.009857961907982826,
-0.00023326018708758056,
0.05467062443494797,
-0.02305912785232067,
-0.010226331651210785,
-0.03083549439907074,
-0.022222300991415977,
-0.00017339499027002603,
0.05563374236226082,
0.0648239403963089,
-0.010882338508963585,
-0.04081147909164429,
-0.004918030928820372,
0.03590252995491028,
0.00002473468703101389,
0.004241401795297861,
0.01952819898724556,
-0.02586541883647442,
0.03046020120382309,
-0.035461898893117905,
-0.028664465993642807,
0.04415817931294441,
0.026279665529727936,
-0.00793527439236641,
0.1405097246170044,
-0.01516327541321516,
-0.04419257491827011,
0.007500193547457457,
0.014620572328567505,
0.016661301255226135,
-0.005307839717715979,
-0.09375684708356857,
-0.013437638059258461,
-0.11099037528038025,
0.006018569227308035,
-0.011437585577368736,
0.0067970724776387215,
0.01614900678396225,
-0.07430160790681839,
0.025786034762859344,
0.00046079311869107187,
-0.10774558037519455,
-0.05846861004829407,
-0.03958709537982941,
-0.04955887421965599,
-0.048097334802150726,
-0.0424819178879261,
0.001589649822562933,
0.04410087689757347,
0.04852516949176788,
-0.049488529562950134,
-0.06266440451145172,
0.018929241225123405,
-0.022314099594950676,
-0.03043101355433464,
-0.04992286115884781,
-0.02206186205148697,
-0.037379950284957886,
0.03555501997470856,
0.015151792205870152,
0.0091982027515769,
0.011456198059022427,
-0.012500258162617683,
-0.0011783117661252618,
0.004677625838667154,
0.002346852095797658,
0.035636335611343384,
0.031302038580179214,
0.03203677758574486,
0.09893865138292313,
-0.007821541279554367,
-0.0002133962989319116,
0.060096658766269684,
0.02155292220413685,
-0.0023159876000136137,
-0.02219555899500847,
-0.08933227509260178,
0.012917476706206799,
0.012795457616448402,
-0.01998046785593033,
0.03605274856090546,
0.005091947037726641,
-0.007930019870400429,
0.010322957299649715,
0.02227906323969364,
-0.0038814665749669075,
-0.002664289204403758,
0.030967364087700844,
0.013102592900395393,
0.0061848885379731655,
-0.03367398679256439,
-0.11326088756322861,
0.042552120983600616,
0.08064528554677963,
-0.2847534418106079,
-0.020848823711276054,
0.024837829172611237,
0.028399242088198662,
0.008261831477284431,
-0.036062631756067276,
0.049000032246112823,
-0.009405418299138546,
-0.05592940375208855,
0.001101936912164092,
-0.008915647864341736,
0.021038277074694633,
0.05769946053624153,
0.0357467457652092,
-0.008134455420076847,
-0.0073604644276201725,
0.037026870995759964,
0.009193633683025837,
-0.04805850610136986,
-0.01266093086451292,
0.017623016610741615,
-0.028915993869304657,
0.12719829380512238,
0.019657503813505173,
-0.0022845640778541565,
0.10814101994037628,
-0.014990209601819515,
0.017966479063034058,
0.020926499739289284,
0.010446889325976372,
-0.028842946514487267,
0.004970837850123644,
0.11191895604133606,
-0.07043550908565521,
0.02529277466237545,
-0.008782495744526386,
-0.029109923169016838,
-0.001234247931279242,
-0.05133994668722153,
-0.022426456212997437,
0.0024133087135851383,
0.050965528935194016,
-0.043764859437942505,
-0.00826170388609171,
0.057448554784059525,
-0.061148401349782944,
-0.0536256842315197,
-0.05187016353011131,
0.002012374345213175,
0.029813487082719803,
-0.02583271451294422,
-0.0024115818087011576,
-0.024448219686746597,
0.023933662101626396,
0.03363192081451416,
0.02055835910141468,
0.010963605716824532,
-0.0016665337607264519,
0.025142131373286247,
-0.058510176837444305,
-0.03063027933239937,
-0.0034568780101835728,
-0.04737357422709465,
0.09226325899362564,
0.006095770746469498
] |
PMID:8298 | [Treatment of hypertrophic obstructive cardiomyopathy with verapamil, a calcium antagonist (author's transl)]. | Cardiac catheterisation with pressure measurements, left-ventricular cine-angiography and selective coronary angiography confirmed the diagnosis of hypertrophic obstructive cardiomyopathy in 20 patients. After a mean observation period of 20 months during which most of them were treated with beta-blockers, verapamil, 480 mg by mouth, was given for an average of 12 months. There was an impressive improvement in symptoms, compared with the state under beta-blocker treatment. There was a significant reduction in the ECG signs of left-ventricular hypertrophy and of the radiologically measured heart volume. Treatment of this condition with verapamil appeared to be superior to that with beta-blockers. | [Treatment of hypertrophic obstructive cardiomyopathy with verapamil, a calcium antagonist (author's transl)]. Cardiac catheterisation with pressure measurements, left-ventricular cine-angiography and selective coronary angiography confirmed the diagnosis of hypertrophic obstructive cardiomyopathy in 20 patients. After a mean observation period of 20 months during which most of them were treated with beta-blockers, verapamil, 480 mg by mouth, was given for an average of 12 months. There was an impressive improvement in symptoms, compared with the state under beta-blocker treatment. There was a significant reduction in the ECG signs of left-ventricular hypertrophy and of the radiologically measured heart volume. Treatment of this condition with verapamil appeared to be superior to that with beta-blockers. | [
0.0010173346381634474,
-0.0034244018606841564,
-0.02990827150642872,
0.034215278923511505,
-0.009708350524306297,
-0.06952054053544998,
-0.04376361891627312,
0.02708914317190647,
0.002291344804689288,
-0.0457427017390728,
0.023454857990145683,
-0.05550915375351906,
0.026784652844071388,
0.039002902805805206,
-0.03424469754099846,
-0.014208989217877388,
0.01662606932222843,
-0.02371876686811447,
-0.004885838367044926,
0.037348996847867966,
-0.026460131630301476,
0.002546844072639942,
-0.017809610813856125,
-0.01411918830126524,
0.028310276567935944,
0.0046050408855080605,
-0.04342732951045036,
-0.011245046742260456,
-0.042918357998132706,
-0.13190051913261414,
-0.04150353744626045,
0.04536183923482895,
0.0683036744594574,
-0.021263347938656807,
-0.031035199761390686,
-0.022139234468340874,
-0.02313988097012043,
0.011111755855381489,
-0.09360010176897049,
0.04256196320056915,
0.03143811225891113,
0.013644728809595108,
0.020057635381817818,
-0.004717596340924501,
0.01422200258821249,
-0.018186643719673157,
-0.026459673419594765,
0.009360160678625107,
0.1354370266199112,
0.03071485459804535,
-0.030555162578821182,
0.005167156457901001,
0.029899509623646736,
0.044433459639549255,
0.015489018522202969,
-0.006440716329962015,
0.014983326196670532,
0.023487765341997147,
0.03460077568888664,
0.016664613038301468,
0.01683075912296772,
0.014076145365834236,
-0.14864888787269592,
-0.0019292084034532309,
-0.019721008837223053,
-0.010515132918953896,
0.022362999618053436,
0.017107512801885605,
0.0638708844780922,
0.10993430018424988,
0.03727526217699051,
0.021751202642917633,
0.04438479617238045,
0.03226843103766441,
-0.020116722211241722,
-0.034638114273548126,
-0.00026582853752188385,
0.005479523912072182,
-0.025427309796214104,
0.029002558439970016,
0.03024597279727459,
-0.012383601628243923,
0.02218867652118206,
-0.09499483555555344,
0.07091140747070312,
-0.017730945721268654,
0.017234371975064278,
-0.057146064937114716,
0.011897895485162735,
-0.052886273711919785,
-0.06015682592988014,
-0.05435603857040405,
0.023938264697790146,
-0.04361319914460182,
-0.05015013739466667,
0.02402247115969658,
0.0016172974137589335,
0.021627265959978104,
-0.04460717737674713,
0.3664354085922241,
0.02855600230395794,
-0.02051626704633236,
-0.06946969777345657,
-0.017086705192923546,
0.025166617706418037,
-0.004937870893627405,
0.015460346825420856,
0.022146031260490417,
0.011093434877693653,
0.03170052915811539,
0.008790837600827217,
-0.058076705783605576,
0.03601687401533127,
-0.01386722456663847,
-0.04121701046824455,
0.0014886672142893076,
0.05235009267926216,
-0.014309627003967762,
-0.041987478733062744,
0.07295212894678116,
-0.02490309812128544,
-0.030408913269639015,
0.03340402990579605,
-0.0025650670286267996,
0.03795761242508888,
-0.011655096895992756,
0.0026893247850239277,
0.02856898494064808,
0.07645779103040695,
0.05560795217752457,
0.062217626720666885,
0.03094562143087387,
-0.07565964758396149,
-0.007252052891999483,
0.0060546183958649635,
0.01134871132671833,
0.057905420660972595,
0.0011541844578459859,
0.07116587460041046,
0.024393856525421143,
-0.024553148075938225,
-0.04327097162604332,
0.014741023071110249,
-0.04884316399693489,
-0.08674292266368866,
0.04159402474761009,
0.007548080291599035,
0.031679410487413406,
-0.044253576546907425,
0.01628251187503338,
-0.012996715493500233,
0.017107974737882614,
-0.012935771606862545,
0.029718471691012383,
-0.05749168246984482,
0.004679962992668152,
0.05945926159620285,
0.07549732178449631,
-0.035312220454216,
0.046058546751737595,
-0.0732712671160698,
-0.04064289480447769,
-0.0038386425003409386,
0.03837449476122856,
-0.06128739193081856,
-0.007952699437737465,
-0.05740931257605553,
-0.029087617993354797,
-0.004953625146299601,
0.022736238315701485,
0.0027721517253667116,
-0.04584455117583275,
0.0438893660902977,
-0.011194520629942417,
0.00419081375002861,
0.03621722757816315,
0.029300164431333542,
-0.09339159727096558,
0.05730690434575081,
-0.008616331964731216,
0.013411874882876873,
0.043589573353528976,
-0.02214036136865616,
0.050268031656742096,
0.04221572354435921,
-0.009571203030645847,
-0.040455251932144165,
-0.03790684789419174,
0.03758899122476578,
0.015633411705493927,
-0.014490192756056786,
-0.006521145347505808,
-0.11461269110441208,
0.03182874619960785,
-0.045953813940286636,
-0.014770802110433578,
-0.066432423889637,
0.05381391569972038,
-0.03324407711625099,
-0.0034282987471669912,
0.07144778966903687,
0.017846906557679176,
0.040536753833293915,
0.06819553673267365,
-0.0002801772207021713,
0.0077798450365662575,
0.006063955835998058,
-0.01790589466691017,
-0.01942378096282482,
0.01751144975423813,
0.017551742494106293,
0.07804694771766663,
0.1005818173289299,
0.028323998674750328,
-0.0697740912437439,
0.06199052929878235,
-0.04708969593048096,
0.02706366404891014,
0.006022262386977673,
-0.01647345907986164,
0.040714822709560394,
0.02126927673816681,
0.008106176741421223,
-0.2974519431591034,
-0.060703735798597336,
-0.006573474034667015,
0.01979128271341324,
0.061326008290052414,
-0.050090763717889786,
0.05249972641468048,
-0.07686903327703476,
-0.042028650641441345,
-0.031156226992607117,
-0.03233378008008003,
0.03965774551033974,
-0.011090702377259731,
0.04044951871037483,
-0.03446802496910095,
0.033537689596414566,
0.10143955796957016,
-0.002526691881939769,
-0.09249167889356613,
-0.04043978825211525,
0.0004489039711188525,
-0.039448902010917664,
-0.009557659737765789,
0.07101210206747055,
0.032523054629564285,
-0.012502790428698063,
0.11333008110523224,
0.008158986456692219,
-0.06002643704414368,
-0.02223745919764042,
-0.02157915197312832,
-0.010610180906951427,
-0.02953370101749897,
-0.08923327177762985,
-0.016761500388383865,
-0.02800406701862812,
0.04294444993138313,
-0.0017656531417742372,
0.013685504905879498,
0.00859030056744814,
-0.0281961839646101,
0.0011401892406865954,
-0.043088868260383606,
0.0054601384326815605,
-0.10627354681491852,
0.02264128252863884,
-0.024394487962126732,
0.007135883439332247,
0.0033847142476588488,
0.030863849446177483,
0.05549132078886032,
0.014254426583647728,
-0.024120308458805084,
0.02567441575229168,
0.05784698575735092,
-0.042382024228572845,
-0.06933977454900742,
-0.02508050575852394,
-0.050652001053094864,
-0.05547573044896126,
-0.03422270342707634,
0.060872673988342285,
0.027901289984583855,
-0.029382631182670593,
-0.01264918316155672,
0.034960754215717316,
0.04790925234556198,
0.033590421080589294,
0.04299190640449524,
0.03894011303782463,
-0.06538672745227814,
0.03915336728096008,
-0.042690131813287735,
-0.01323314756155014,
0.0942743718624115,
0.06449075788259506,
-0.020943861454725266,
-0.01874954253435135,
-0.017585670575499535,
-0.015990253537893295,
0.019543103873729706,
0.03111867420375347,
0.050036847591400146,
0.019293226301670074,
-0.0641782358288765,
-0.06481126695871353,
-0.013583513908088207,
0.03434788063168526,
-0.002020721323788166,
0.02773049660027027,
-0.035706453025341034,
0.055183399468660355,
-0.054388467222452164,
-0.020666087046265602,
-0.0010129495058208704,
0.04260864108800888,
-0.30332431197166443,
0.01906796358525753,
0.04468278959393501,
-0.018481239676475525,
-0.026881566271185875,
0.03822647035121918,
0.04453377053141594,
-0.002050170209258795,
-0.04118941351771355,
-0.008964254520833492,
-0.038785893470048904,
0.013890841975808144,
0.06896237283945084,
-0.008579680696129799,
-0.00929285679012537,
-0.05203310400247574,
0.04619021713733673,
-0.04751475900411606,
0.06850001215934753,
-0.0899212509393692,
0.012206603772938251,
0.014669771306216717,
0.1165400967001915,
0.014667925424873829,
-0.05073787644505501,
0.03512490913271904,
0.012913712300360203,
0.04756542295217514,
-0.010728140361607075,
-0.008457849733531475,
-0.005753825884312391,
0.03820996358990669,
0.06115081533789635,
-0.022566521540284157,
0.013566968962550163,
0.028775354847311974,
-0.0028459536843001842,
0.014180977828800678,
-0.02859407663345337,
-0.005751640070229769,
0.05719846859574318,
0.039098914712667465,
-0.05686351656913757,
-0.01762724295258522,
0.06036953255534172,
-0.06700112670660019,
-0.04884684085845947,
0.029604976996779442,
0.015635255724191666,
0.01709805056452751,
-0.045911140739917755,
-0.01041784230619669,
0.05229203775525093,
0.04039312154054642,
-0.03544923663139343,
-0.020352015271782875,
-0.031241070479154587,
0.0067930324003100395,
0.0442669540643692,
-0.07749933004379272,
0.00020363055227790028,
-0.0009428223129361868,
-0.046912141144275665,
0.07853437215089798,
0.02572472020983696
] |
PMID:8296 | Effect of 3-methylcholanthrene pretreatment on the bioavailability of phenacetin in the rat. | A thin-layer chromatographic procedure is described for the quantitative determination of phenacetin and acetaminophen in rat plasma. The method was used to determine the effect of 3-methylcholanthrene (3-MC) on the disposition and bioavailability of phenacetin following its oral and iv administration to rats. Pretreatment with 3-MC decreased the plasma half-life of phenacetin, after iv administration, from 28 min to 4.5 min and reduced the systemic bioavailability of phenacetin, after oral administration, from 45% in control rats to 6% in 3-MC-treated rats. By comparing the plasma levels of phenacetin in the portal circulation with those in the peripheral circulation, following the oral administration of phenacetin, it was concluded that the 7-fold reduction in the bioavailability of phenacetin observed in 3-MC treated rats was caused by a marked increase in the metabolism of phenacetin during its first pass through the liver. | Effect of 3-methylcholanthrene pretreatment on the bioavailability of phenacetin in the rat. A thin-layer chromatographic procedure is described for the quantitative determination of phenacetin and acetaminophen in rat plasma. The method was used to determine the effect of 3-methylcholanthrene (3-MC) on the disposition and bioavailability of phenacetin following its oral and iv administration to rats. Pretreatment with 3-MC decreased the plasma half-life of phenacetin, after iv administration, from 28 min to 4.5 min and reduced the systemic bioavailability of phenacetin, after oral administration, from 45% in control rats to 6% in 3-MC-treated rats. By comparing the plasma levels of phenacetin in the portal circulation with those in the peripheral circulation, following the oral administration of phenacetin, it was concluded that the 7-fold reduction in the bioavailability of phenacetin observed in 3-MC treated rats was caused by a marked increase in the metabolism of phenacetin during its first pass through the liver. | [
-0.020169150084257126,
0.02241463214159012,
-0.010442432947456837,
-0.007398130372166634,
0.09029196947813034,
-0.02293476089835167,
0.03130326420068741,
0.011646612547338009,
-0.05581117048859596,
-0.03091890923678875,
0.025997750461101532,
-0.040742211043834686,
-0.013331744819879532,
0.009006982669234276,
-0.010819623246788979,
-0.0010079331696033478,
0.05413603410124779,
0.018122369423508644,
-0.056939586997032166,
-0.03537974879145622,
-0.020110439509153366,
-0.04229940101504326,
0.016056908294558525,
0.05020635202527046,
0.06180392950773239,
0.02066844142973423,
0.008876374922692776,
-0.011876610107719898,
-0.07646592706441879,
-0.21726028621196747,
0.04840681701898575,
-0.002448562067002058,
-0.019522303715348244,
-0.03467205539345741,
-0.004346136003732681,
0.010435053147375584,
0.0020706846844404936,
0.048831406980752945,
0.06428231298923492,
0.06921540945768356,
0.07377339899539948,
0.05270787701010704,
-0.011438881047070026,
0.051605358719825745,
-0.01252276822924614,
-0.00947240274399519,
-0.04926496371626854,
0.004319076891988516,
0.14997273683547974,
-0.014212769456207752,
0.007484260480850935,
-0.01835894025862217,
0.05104783549904823,
0.023281728848814964,
0.026536544784903526,
0.01815088279545307,
-0.0022183754481375217,
-0.008837816305458546,
-0.023603234440088272,
0.05580420792102814,
0.0154607854783535,
0.026450717821717262,
-0.18028970062732697,
0.08954684436321259,
0.03799605742096901,
-0.010595017112791538,
-0.011918162927031517,
-0.028970545157790184,
0.02960522100329399,
0.07744324207305908,
-0.09998633712530136,
-0.0014374247984960675,
-0.0638793557882309,
-0.011401735246181488,
0.037658996880054474,
-0.0823361873626709,
0.04120802506804466,
-0.056007660925388336,
0.01231675036251545,
-0.018850039690732956,
0.039319004863500595,
0.018620673567056656,
0.013714222237467766,
-0.07942071557044983,
-0.002191173145547509,
-0.019842097535729408,
-0.04122788459062576,
-0.013212276622653008,
0.0009823227301239967,
-0.00538276694715023,
-0.0026951516047120094,
-0.054258834570646286,
-0.02656674012541771,
-0.021118292585015297,
-0.031363390386104584,
-0.012239072471857071,
-0.02370816096663475,
-0.03868371620774269,
-0.030479872599244118,
0.3105331361293793,
0.01129151415079832,
0.049763474613428116,
-0.0945863127708435,
-0.0327267125248909,
-0.029863940551877022,
0.04541413486003876,
-0.017528943717479706,
-0.00832656491547823,
0.06409736722707748,
0.023404262959957123,
0.06560148298740387,
0.030301449820399284,
0.036304693669080734,
-0.024536853656172752,
-0.00474578095600009,
0.019539739936590195,
0.03485989198088646,
0.04602990299463272,
0.006863373797386885,
-0.0023170269560068846,
0.017148645594716072,
0.01763169839978218,
0.05726766958832741,
0.027142010629177094,
0.014116364531219006,
-0.0025952791329473257,
0.05911708250641823,
0.09994256496429443,
0.023126306012272835,
0.004644407890737057,
0.03596069663763046,
-0.02558894269168377,
-0.06262741982936859,
-0.00017887676949612796,
-0.0013152341125532985,
-0.02313525788486004,
-0.0024865244049578905,
-0.0598975270986557,
0.019654100760817528,
0.04042532667517662,
-0.03923211991786957,
-0.0716887041926384,
-0.004601072054356337,
-0.06042817234992981,
-0.07092683017253876,
0.12599797546863556,
0.04860430955886841,
0.015065907500684261,
0.005701090674847364,
-0.0022540362551808357,
0.05249296501278877,
0.062266185879707336,
-0.013207132928073406,
0.04700367897748947,
-0.041799210011959076,
0.020538032054901123,
-0.00655943900346756,
-0.058672357350587845,
-0.00860700011253357,
0.010628505609929562,
-0.07769499719142914,
-0.04801720753312111,
-0.027838872745633125,
0.10906998813152313,
0.002494912827387452,
0.014193627052009106,
0.0169607475399971,
0.04322180524468422,
0.009324015118181705,
-0.01479560136795044,
0.0058277687057852745,
-0.038493651896715164,
-0.01502447109669447,
-0.0013776447158306837,
-0.06430453062057495,
0.003410419449210167,
0.005643503740429878,
0.0088764363899827,
0.05280482396483421,
-0.03243313357234001,
0.036280542612075806,
0.04770292341709137,
-0.05325482040643692,
-0.026895983144640923,
0.015517132356762886,
-0.020316388458013535,
-0.046510789543390274,
0.0796404555439949,
-0.003346152836456895,
0.03225519880652428,
-0.018595999106764793,
0.020683528855443,
-0.10080835223197937,
0.0021156880538910627,
-0.041509415954351425,
-0.021873068064451218,
-0.050252608954906464,
0.032266370952129364,
-0.0030912787187844515,
-0.015050990507006645,
0.12674738466739655,
0.04708307236433029,
0.019020969048142433,
0.057297736406326294,
0.045368555933237076,
0.054165419191122055,
-0.0003911248059011996,
-0.011676537804305553,
0.04398229345679283,
-0.013065093196928501,
0.0011812334414571524,
0.013525144197046757,
0.009032828733325005,
0.027415907010436058,
0.003407096490263939,
0.05140111595392227,
0.017817672342061996,
0.01338107604533434,
0.07112748920917511,
0.005144656635820866,
-0.002074912888929248,
-0.03825374320149422,
-0.0071957847103476524,
-0.2717328667640686,
0.04112907871603966,
0.03582698479294777,
-0.004798701498657465,
0.06432122737169266,
-0.07371239364147186,
0.018711112439632416,
-0.05736125633120537,
-0.0036754393950104713,
-0.08663253486156464,
0.046567369252443314,
0.0661393404006958,
-0.041809916496276855,
0.029719071462750435,
-0.005878358148038387,
0.029241180047392845,
0.006817350629717112,
0.0014313687570393085,
0.023885086178779602,
-0.0022693315986543894,
0.02419157698750496,
-0.03790993615984917,
-0.03267597779631615,
-0.005443620961159468,
0.06243694946169853,
0.017219889909029007,
0.10136992484331131,
-0.037338174879550934,
-0.08268088102340698,
-0.056436918675899506,
0.01634809747338295,
0.0034442536998540163,
-0.01646275818347931,
-0.09592143446207047,
0.025826459750533104,
-0.03961186483502388,
-0.026165293529629707,
-0.05296093225479126,
-0.017983969300985336,
-0.020504487678408623,
-0.04615027830004692,
0.0455460362136364,
0.06207452714443207,
-0.09203591197729111,
-0.09993650019168854,
-0.0431227907538414,
-0.018979132175445557,
0.048271916806697845,
-0.004099569749087095,
-0.0051092603243887424,
-0.04412992671132088,
0.02663186937570572,
-0.01633515954017639,
0.02428681030869484,
0.029783107340335846,
0.02873004972934723,
-0.04110870882868767,
0.04882737621665001,
-0.06918095797300339,
0.03237876668572426,
0.002054789336398244,
-0.011262853629887104,
-0.030574360862374306,
-0.006944486405700445,
0.00830468162894249,
0.0017466834979131818,
0.0044057187624275684,
-0.03305429965257645,
-0.01917865127325058,
0.05197687819600105,
0.07807964831590652,
0.10803103446960449,
-0.0030764942057430744,
0.057418037205934525,
0.03717650845646858,
0.03487725555896759,
-0.012483391910791397,
-0.035511501133441925,
-0.03383619338274002,
-0.00246511772274971,
-0.06032782793045044,
-0.033135298639535904,
0.03997260704636574,
-0.04943456873297691,
-0.013484231196343899,
0.02136283740401268,
-0.007533484138548374,
0.02626298926770687,
0.0448470264673233,
0.028012365102767944,
-0.03635639697313309,
0.022261399775743484,
0.00873008742928505,
-0.05731027573347092,
0.06362742930650711,
0.0011988523183390498,
-0.259829044342041,
0.02194128930568695,
0.004109470639377832,
0.02828541211783886,
0.02638799510896206,
0.023929372429847717,
0.041950613260269165,
-0.01388947106897831,
-0.04137502983212471,
-0.026421183720231056,
0.025802316144108772,
0.041678689420223236,
0.07879332453012466,
-0.006738994736224413,
-0.01542870793491602,
-0.008171739056706429,
0.03466477245092392,
-0.03489961102604866,
-0.036250464618206024,
-0.06287303566932678,
0.02404286339879036,
-0.07307992875576019,
0.10269765555858612,
0.013931107707321644,
-0.08680080622434616,
-0.005264577455818653,
-0.0021115103736519814,
0.059725165367126465,
-0.05054676905274391,
0.0199216827750206,
-0.0540650337934494,
-0.02222052589058876,
0.09619969874620438,
-0.05228310450911522,
0.05815409496426582,
0.04019354283809662,
-0.07533828914165497,
-0.05386876314878464,
-0.01444072462618351,
0.06619735807180405,
-0.040280409157276154,
-0.025198595598340034,
-0.006925612688064575,
-0.02235306240618229,
0.033970583230257034,
-0.06863684207201004,
-0.008105075918138027,
-0.010681966319680214,
-0.016119476407766342,
0.01577381044626236,
-0.040556326508522034,
0.016205186024308205,
0.003263985738158226,
0.009221994318068027,
-0.06172192841768265,
0.014389866963028908,
-0.03003798797726631,
-0.02131575345993042,
0.012370931915938854,
-0.062178079038858414,
0.0031415573321282864,
0.019697852432727814,
-0.016172371804714203,
0.06186973676085472,
0.0702696293592453
] |
PMID:8297 | Effect of methadone dose on the biliary excretion of methadone metabolites in the rat. | The effect of three different doses of 14C-methadone (0.08, 1.0 and 2.5 mg/kg) on the biliary excretion of methadone metabolites was studied in the rat. After administration of the 0.08- and 1.0-mg/kg doses of 14C-methadone there was no difference in the percentage of administered 14C excreted into bile with time. However, after the 2.5-mg/kg dose a significant increase was observed in the percentage of administered 14C excreted into bile. Analysis of bile samples showed that this increase was due to increases in the biliary excretion of two of the major metabolites of methadone. Several mechanisms could be responsible for this disproportionate increase in biliary excretion of methadone metabolites after high doses of methadone. It was found that the effect of high methadone dose on the biliary excretion of its metabolites was nearly eliminated when studied in phenobarbital (PB)-pretreated rats. Pretreatment of rats with PB increases the biliary excretion of methadone metabolites, primarily by increasing rates of methadone metabolism. The lack of additivity of the effect of the high dose of methadone and PB pretreatment on the biliary excretion of methadone metabolites suggests that a high dose of methadone also stimulates methadone metabolism, which results in the observed increased percentage of the administered dose excreted into bile. | Effect of methadone dose on the biliary excretion of methadone metabolites in the rat. The effect of three different doses of 14C-methadone (0.08, 1.0 and 2.5 mg/kg) on the biliary excretion of methadone metabolites was studied in the rat. After administration of the 0.08- and 1.0-mg/kg doses of 14C-methadone there was no difference in the percentage of administered 14C excreted into bile with time. However, after the 2.5-mg/kg dose a significant increase was observed in the percentage of administered 14C excreted into bile. Analysis of bile samples showed that this increase was due to increases in the biliary excretion of two of the major metabolites of methadone. Several mechanisms could be responsible for this disproportionate increase in biliary excretion of methadone metabolites after high doses of methadone. It was found that the effect of high methadone dose on the biliary excretion of its metabolites was nearly eliminated when studied in phenobarbital (PB)-pretreated rats. Pretreatment of rats with PB increases the biliary excretion of methadone metabolites, primarily by increasing rates of methadone metabolism. The lack of additivity of the effect of the high dose of methadone and PB pretreatment on the biliary excretion of methadone metabolites suggests that a high dose of methadone also stimulates methadone metabolism, which results in the observed increased percentage of the administered dose excreted into bile. | [
-0.027384696528315544,
-0.017317065969109535,
0.05063920095562935,
-0.0002868599258363247,
0.042676132172346115,
-0.026990944519639015,
0.02853318117558956,
0.00926986988633871,
-0.03066560998558998,
-0.02485962212085724,
0.03813650459051132,
-0.05794790759682655,
0.018566565588116646,
0.06288528442382812,
-0.027930237352848053,
0.031218361109495163,
0.05105435475707054,
-0.006928596645593643,
-0.041039302945137024,
0.031483788043260574,
-0.002602833090350032,
0.0012944150948897004,
0.03688529506325722,
-0.011319251731038094,
0.01615823246538639,
0.01460343785583973,
-0.010478458367288113,
-0.003931667190045118,
-0.051079779863357544,
-0.17721113562583923,
0.03878045082092285,
0.06268735229969025,
0.034311529248952866,
-0.020717434585094452,
-0.009145309217274189,
-0.007701640482991934,
0.024835500866174698,
0.04728183522820473,
0.017489049583673477,
0.09838059544563293,
0.037886057049036026,
0.05117616429924965,
0.0021487257909029722,
0.04346923157572746,
-0.052531708031892776,
-0.011062337085604668,
-0.08117859810590744,
-0.016428599134087563,
0.16576150059700012,
-0.00891290232539177,
0.04454430565237999,
-0.04756000638008118,
-0.016002709046006203,
0.01501527987420559,
-0.011335855349898338,
-0.034344032406806946,
-0.010436468757689,
0.05060046538710594,
-0.06812998652458191,
0.05808259919285774,
-0.011923904530704021,
0.018984267488121986,
-0.14355967938899994,
0.03471236675977707,
0.05259186029434204,
0.008067231625318527,
-0.056015495210886,
-0.06773614883422852,
0.043720826506614685,
0.06295570731163025,
-0.0679997056722641,
0.025415964424610138,
-0.0702926516532898,
0.013417384587228298,
0.04863094910979271,
-0.060537610203027725,
-0.009943283163011074,
-0.04111983999609947,
0.00917498767375946,
-0.022001013159751892,
-0.008542963303625584,
0.03264208137989044,
0.019135236740112305,
0.023688705638051033,
0.015814291313290596,
-0.06008164584636688,
-0.038727447390556335,
-0.023266682401299477,
0.03143077343702316,
0.03722432255744934,
-0.005621856078505516,
0.013857566751539707,
-0.03434430807828903,
-0.03352855518460274,
-0.07039966434240341,
0.008239594288170338,
0.039923228323459625,
-0.012033769860863686,
-0.022080767899751663,
0.35419031977653503,
-0.002732248743996024,
0.00910075195133686,
-0.06807885318994522,
-0.031792521476745605,
-0.0063452087342739105,
0.051009651273489,
0.052841536700725555,
-0.01178411953151226,
-0.004124512430280447,
0.05458964407444,
0.055242981761693954,
0.026213109493255615,
0.013117033988237381,
-0.0059014782309532166,
-0.05944278463721275,
-0.002995990216732025,
0.02361738681793213,
-0.021548541262745857,
-0.026279529556632042,
0.003368370234966278,
-0.013075562193989754,
0.015870708972215652,
0.035684604197740555,
-0.005745847709476948,
-0.0038597933016717434,
0.0067626251839101315,
0.012050420977175236,
0.1106695681810379,
-0.03013007342815399,
0.011426243931055069,
0.005605215672403574,
0.0288180373609066,
0.03234682232141495,
0.011347590945661068,
-0.0066269212402403355,
0.01335417665541172,
0.04401779547333717,
0.022386759519577026,
0.014485834166407585,
0.036259207874536514,
-0.010831688530743122,
-0.09478362649679184,
-0.044942427426576614,
-0.09789971262216568,
-0.0470530204474926,
0.0916638895869255,
0.046208757907152176,
0.0021634837612509727,
-0.02799621783196926,
-0.030866064131259918,
0.01130048930644989,
0.014698237180709839,
-0.0033449032343924046,
0.035852205008268356,
-0.0546041801571846,
0.018626946955919266,
0.012377609498798847,
0.029079314321279526,
0.006759077310562134,
-0.010928155854344368,
-0.04817204922437668,
-0.0368841290473938,
0.002540384419262409,
0.07300299406051636,
0.03513490408658981,
0.05063450708985329,
-0.04752993583679199,
0.00430092541500926,
-0.015568486414849758,
-0.020595025271177292,
-0.0027607756201177835,
-0.00979598332196474,
-0.04702368751168251,
-0.03744897246360779,
-0.01469013188034296,
-0.020893020555377007,
0.0412963405251503,
0.04883887618780136,
0.031193537637591362,
0.00349844666197896,
0.0144247617572546,
-0.009518379345536232,
-0.06687571108341217,
0.057669587433338165,
-0.02585943229496479,
0.03910986706614494,
-0.03774826601147652,
0.0003694382612593472,
-0.05374416336417198,
0.0419282540678978,
-0.06798277795314789,
0.03483497351408005,
-0.12808860838413239,
-0.07783876359462738,
-0.032249607145786285,
-0.047462958842515945,
-0.10575711727142334,
0.053371042013168335,
-0.025128517299890518,
0.0030980450101196766,
0.08764444291591644,
0.06498311460018158,
0.027263037860393524,
0.031456299126148224,
-0.028839226812124252,
0.023402303457260132,
0.00728664780035615,
-0.01695242151618004,
0.004896230064332485,
-0.0317639485001564,
-0.0017549579497426748,
-0.021247567608952522,
0.026300808414816856,
0.0352015495300293,
0.007048366591334343,
0.024671081453561783,
-0.03684660792350769,
-0.033601634204387665,
-0.004126600455492735,
0.021412570029497147,
0.03268546983599663,
-0.06337177753448486,
0.021032491698861122,
-0.2781486213207245,
-0.03570930287241936,
0.03609820082783699,
0.05319821462035179,
0.06282104551792145,
-0.041824039071798325,
-0.015874914824962616,
0.035795118659734726,
-0.038803908973932266,
-0.0115163279697299,
0.04196580871939659,
0.031036922708153725,
-0.019941451027989388,
-0.025193383917212486,
-0.03652537241578102,
0.0018467303598299623,
0.008878057822585106,
-0.026292551308870316,
0.0364333875477314,
-0.02446429803967476,
-0.04666437953710556,
-0.04498645290732384,
-0.018506744876503944,
0.02148590423166752,
0.06915564835071564,
-0.00610120827332139,
0.08897574245929718,
0.007906408049166203,
-0.09142853319644928,
0.0005494158831425011,
0.04699984937906265,
-0.03576076775789261,
0.043402209877967834,
-0.12138786911964417,
0.007269981782883406,
-0.04436366260051727,
0.056116990745067596,
-0.026814011856913567,
-0.009111185558140278,
-0.01707015372812748,
-0.06445703655481339,
-0.012556045316159725,
-0.010280139744281769,
-0.08691252022981644,
-0.06514258682727814,
0.0010404024505987763,
0.0036697606556117535,
0.058717597275972366,
-0.025596171617507935,
-0.016108805313706398,
0.027321264147758484,
0.04077927768230438,
0.01617276296019554,
-0.07958676666021347,
0.024039005860686302,
-0.04382673278450966,
-0.0010371654061600566,
-0.07331991195678711,
-0.05011342838406563,
-0.02674335613846779,
-0.025583265349268913,
-0.01714000105857849,
0.015003997832536697,
-0.050728071480989456,
0.04294046014547348,
0.0524430014193058,
-0.01913371868431568,
0.006923021748661995,
0.0402863547205925,
0.03774333745241165,
0.0579664409160614,
0.11995033174753189,
-0.01967712864279747,
-0.014030419290065765,
0.07660296559333801,
0.0071530696004629135,
-0.04010673984885216,
-0.06129615753889084,
-0.09508972615003586,
0.012349431402981281,
0.005483606830239296,
-0.033727873116731644,
0.06296148151159286,
-0.0013745102332904935,
0.016931887716054916,
0.04966478794813156,
-0.04518596827983856,
-0.01060484629124403,
0.057620808482170105,
-0.0031899712048470974,
-0.015802202746272087,
-0.004878397565335035,
0.005113217048346996,
-0.0705203115940094,
0.02764536440372467,
0.08943790942430496,
-0.29222187399864197,
0.007901941426098347,
0.012691326439380646,
-0.015559358522295952,
0.098158098757267,
-0.0003437600389588624,
0.0337042436003685,
0.0332995243370533,
-0.0337635762989521,
-0.003945693373680115,
0.03642173111438751,
0.02536761574447155,
0.06277869641780853,
0.01139740739017725,
-0.02492772974073887,
-0.01188624743372202,
0.0420522578060627,
0.024636579677462578,
-0.0037165151443332434,
-0.018472304567694664,
0.019320998340845108,
-0.06953126937150955,
0.07774066179990768,
0.006624395959079266,
-0.04805530980229378,
0.03152850642800331,
0.02858731709420681,
0.0704762190580368,
0.0424472838640213,
0.020647959783673286,
0.014728015288710594,
0.05023306608200073,
0.016972824931144714,
0.006679720710963011,
0.004770027007907629,
0.03558145835995674,
-0.05819745361804962,
0.046755220741033554,
-0.00187748484313488,
0.03622053936123848,
0.000820341520011425,
0.015668559819459915,
-0.017084376886487007,
-0.017306020483374596,
0.044533565640449524,
-0.10582518577575684,
-0.01747671701014042,
-0.05406268313527107,
0.06196311488747597,
0.01002564374357462,
-0.0012007340556010604,
0.0026953285560011864,
-0.034011080861091614,
0.0874360203742981,
0.02206006832420826,
0.0015351551119238138,
-0.018008282408118248,
-0.0029823489021509886,
0.05288013815879822,
-0.043666157871484756,
-0.01574431173503399,
-0.02164778858423233,
0.0023914834018796682,
0.06596364825963974,
-0.018929991871118546
] |
PMID:8301 | [Studies on optimising rubella virus haemagglutination-inhibitor tests]. | Immuno-electrophoretic and serological studies were undertaken to characterise the non-specific serum inhibitor of rubella haemagglutination. The majority of such inhibitor effects come from the beta(1)-lipoproteins. In addition, small inhibitory effects which were due to alpha(1)-lipoproteins were noted in some sera. The result of the tests is influenced less by removing the inhibitor than by conditions of incubation of the serum-antigen mixture, especially pH. The test is more sensitive if it is not performed at optimal pH for haemagglutination. | [Studies on optimising rubella virus haemagglutination-inhibitor tests]. Immuno-electrophoretic and serological studies were undertaken to characterise the non-specific serum inhibitor of rubella haemagglutination. The majority of such inhibitor effects come from the beta(1)-lipoproteins. In addition, small inhibitory effects which were due to alpha(1)-lipoproteins were noted in some sera. The result of the tests is influenced less by removing the inhibitor than by conditions of incubation of the serum-antigen mixture, especially pH. The test is more sensitive if it is not performed at optimal pH for haemagglutination. | [
-0.06455232948064804,
0.021881354972720146,
-0.023256491869688034,
0.02820039540529251,
0.010430482216179371,
0.005114864557981491,
0.07998177409172058,
0.10886776447296143,
-0.03696422278881073,
-0.057352058589458466,
0.03816796839237213,
-0.040628381073474884,
0.03955044597387314,
-0.014011481776833534,
-0.07103529572486877,
-0.06771371513605118,
0.0187759418040514,
-0.026233354583382607,
-0.07438449561595917,
0.06165981665253639,
-0.0661395788192749,
0.017429543659090996,
0.021437522023916245,
-0.001980785746127367,
0.060124631971120834,
0.03463086113333702,
0.007159036584198475,
0.01159543264657259,
-0.0654168352484703,
-0.16012722253799438,
0.03181314095854759,
-0.06349541246891022,
-0.03297837823629379,
-0.016675980761647224,
-0.019005676731467247,
0.03829481080174446,
-0.012935598380863667,
0.028039241209626198,
-0.030414141714572906,
-0.02846345491707325,
0.01697315089404583,
0.009579991921782494,
-0.05270501226186752,
0.04124478995800018,
-0.022395076230168343,
-0.06257735937833786,
-0.023991703987121582,
0.035066187381744385,
0.049596190452575684,
0.036725059151649475,
-0.06117883324623108,
0.009910308755934238,
0.05020463094115257,
0.020760562270879745,
-0.01222282461822033,
-0.009630922228097916,
-0.0355529859662056,
0.02499096840620041,
-0.058539118617773056,
0.0012594620930030942,
-0.059969086199998856,
0.007113088853657246,
-0.10209135711193085,
0.04008611664175987,
0.039178263396024704,
-0.01578417792916298,
0.003424528520554304,
-0.013177100569009781,
0.08792896568775177,
0.06538304686546326,
-0.061589229851961136,
0.08660421520471573,
-0.06121893599629402,
0.018468428403139114,
-0.018173687160015106,
0.006786134093999863,
-0.027057630941271782,
0.008752398192882538,
0.07166646420955658,
0.04241582006216049,
-0.06664565950632095,
-0.025439344346523285,
0.012579751200973988,
-0.0012671233853325248,
0.013770376332104206,
0.04552076384425163,
0.02923542819917202,
-0.002823364222422242,
0.03811399266123772,
-0.029853008687496185,
0.03510158136487007,
-0.03882531449198723,
-0.04274200648069382,
-0.016561057418584824,
-0.03303610906004906,
0.010859210975468159,
-0.013574355281889439,
-0.07087519764900208,
0.0364006832242012,
0.4084545373916626,
-0.06457406282424927,
-0.028973381966352463,
-0.01037879753857851,
-0.03234750032424927,
0.01738244853913784,
-0.026515601202845573,
-0.003887665458023548,
-0.05263644456863403,
-0.005371734034270048,
0.025604454800486565,
0.02315385267138481,
0.04358427971601486,
0.023161759600043297,
-0.0352252721786499,
0.004966590087860823,
0.011214738711714745,
0.021840129047632217,
0.030605202540755272,
-0.06347300112247467,
0.06273188441991806,
-0.002241661539301276,
0.00557755259796977,
0.07822014391422272,
-0.030661333352327347,
0.08631568402051926,
0.018161427229642868,
0.03909340128302574,
0.11601091176271439,
0.0297691747546196,
-0.041301991790533066,
0.0525057315826416,
-0.011802616529166698,
-0.03014434315264225,
-0.02499588578939438,
0.03108120523393154,
0.0286781657487154,
0.0537521094083786,
0.07481227070093155,
0.049571797251701355,
0.05427999421954155,
-0.029402567073702812,
-0.06282753497362137,
-0.0785781666636467,
-0.05514265596866608,
0.01270130556076765,
0.06627316772937775,
-0.012383954599499702,
0.06277777254581451,
0.006647876463830471,
-0.07574636489152908,
-0.017345551401376724,
0.03054153360426426,
-0.034203093498945236,
0.016218915581703186,
-0.04406397044658661,
0.01066482812166214,
0.010262795723974705,
0.015431327745318413,
-0.02413855493068695,
-0.006679944694042206,
-0.08698084950447083,
-0.08242876827716827,
0.0391598679125309,
0.03269907459616661,
-0.04424121975898743,
-0.005331108812242746,
0.01857256516814232,
-0.020346805453300476,
-0.0076230792328715324,
-0.032095830887556076,
-0.03190508857369423,
-0.014285658486187458,
-0.07088682055473328,
-0.024341963231563568,
-0.036128103733062744,
-0.04063635319471359,
-0.022862229496240616,
0.03001442179083824,
-0.017458638176321983,
0.011321603320538998,
-0.012299454770982265,
0.03965788707137108,
-0.015586528927087784,
0.03290163353085518,
0.01672806590795517,
0.03298738971352577,
-0.05050479993224144,
0.012433555908501148,
0.008637575432658195,
0.024948589503765106,
-0.05697355419397354,
-0.023859184235334396,
-0.07595666497945786,
0.0007939371280372143,
0.02187913842499256,
-0.03169385716319084,
-0.03566102311015129,
0.016724366694688797,
0.0006417009280994534,
-0.07854941487312317,
0.09458764642477036,
0.009799298830330372,
0.058992426842451096,
-0.029729116708040237,
0.030498605221509933,
0.04928160458803177,
0.03983880952000618,
-0.020132876932621002,
0.06720498204231262,
-0.033935174345970154,
-0.016784021630883217,
0.04332989454269409,
-0.06852873414754868,
0.002241401467472315,
-0.017106294631958008,
0.07225476950407028,
-0.005168046802282333,
-0.019234955310821533,
0.07350422441959381,
0.010910923592746258,
-0.0006017910782247782,
0.00225437362678349,
-0.05245741829276085,
-0.27472779154777527,
0.002290192525833845,
0.019103683531284332,
-0.021062996238470078,
0.01821613684296608,
-0.027875544503331184,
-0.0020477722864598036,
0.01979002356529236,
-0.0012620077468454838,
-0.012829047627747059,
0.05737323313951492,
0.027096617966890335,
-0.02901632711291313,
-0.044622235000133514,
-0.0016902151983231306,
0.017353659495711327,
-0.030420713126659393,
-0.015777336433529854,
0.02251148596405983,
-0.010784194804728031,
0.02080804668366909,
0.01127629168331623,
0.017747782170772552,
0.0216923076659441,
0.06430038064718246,
-0.05472235754132271,
0.07061820477247238,
0.013949346728622913,
-0.009621946141123772,
-0.035344064235687256,
-0.02865397185087204,
0.07667036354541779,
0.053275130689144135,
-0.046496499329805374,
-0.035627447068691254,
0.03311555087566376,
-0.07002915441989899,
-0.03287424519658089,
0.009362314827740192,
0.023209895938634872,
0.054214052855968475,
0.006250261794775724,
-0.014544458128511906,
-0.052578218281269073,
-0.032914768904447556,
-0.022735033184289932,
0.017542090266942978,
0.02454812079668045,
0.04793260619044304,
0.05131697654724121,
0.01902083493769169,
-0.038008034229278564,
0.043374788016080856,
0.033931612968444824,
0.06964721530675888,
-0.033065278083086014,
-0.05424332246184349,
0.018690673634409904,
-0.037612348794937134,
0.017184128984808922,
0.02767636626958847,
-0.02948768064379692,
0.020859988406300545,
-0.014064677990972996,
-0.012860527262091637,
-0.04178661108016968,
0.0398346483707428,
-0.015519051812589169,
0.04090704396367073,
0.07171837985515594,
-0.018579671159386635,
0.11536049097776413,
-0.01316795963793993,
0.005787370726466179,
0.02768155373632908,
0.05687770992517471,
0.016632845625281334,
-0.06593690067529678,
-0.021533802151679993,
-0.004007166717201471,
0.0063383919186890125,
-0.01966788060963154,
-0.008612049743533134,
-0.020191073417663574,
0.012116578407585621,
0.018936708569526672,
-0.03762742504477501,
-0.05909474939107895,
-0.008329803124070168,
-0.0025103003717958927,
0.0036917252000421286,
-0.04268546402454376,
-0.03411233425140381,
-0.013960588723421097,
-0.02551901526749134,
0.0315866619348526,
-0.28608939051628113,
-0.007797522004693747,
0.01154331024736166,
-0.008809239603579044,
0.058596041053533554,
0.009128953330218792,
-0.019836008548736572,
-0.06428942829370499,
0.011802212335169315,
-0.011653197929263115,
0.03648402914404869,
0.011807053349912167,
0.06630107760429382,
-0.008825963363051414,
-0.004173107910901308,
0.004411530215293169,
0.03850696608424187,
-0.004414036404341459,
0.015882546082139015,
-0.03416472300887108,
0.04007570445537567,
-0.018059076741337776,
0.19105906784534454,
-0.018274707719683647,
-0.04063376784324646,
0.05958089232444763,
-0.0022262088023126125,
-0.024279873818159103,
-0.09280113130807877,
-0.007048614788800478,
0.04358108714222908,
0.004801918752491474,
0.047571055591106415,
0.04338332638144493,
0.02086634747684002,
0.021755943074822426,
0.01270669512450695,
-0.029840542003512383,
-0.03407388553023338,
0.04835481196641922,
0.019948793575167656,
0.03915391489863396,
-0.03921058028936386,
-0.037576161324977875,
0.058874741196632385,
-0.02448679879307747,
-0.03252793475985527,
-0.033651843667030334,
-0.031201135367155075,
-0.031443990767002106,
-0.03042547032237053,
-0.09229451417922974,
0.0478280633687973,
0.023432327434420586,
0.07576297968626022,
-0.057344432920217514,
-0.002721591154113412,
0.0380583256483078,
0.049495961517095566,
0.016580261290073395,
0.03884470462799072,
0.020358901470899582,
-0.03603474795818329,
0.05001774802803993,
0.04177656024694443
] |
PMID:8306 | Inactivation of porcine calcitonin by rat kidney microsome. | Biological activity of porcine calcitonin was most actively inactivated by the rat kidney homogenate than by other tissue homogenates. Among the various subcellular fractions of the rat kidney homogenate examined, microsome fraction was most active in the in vitro inactivation of porcine calcitonin. Inactivation of porcine calcitonin by the rat kidney microsome was dependent on pH and temperature. Inactivating activity of the rat kidney microsome was inhibited by 1 X 10(-3) M monoiodoacetate and 1 X10(-5) M p-chloromercuribenzoate. These results suggest that porcine calcitonin is probably inactivated by a SH-enzyme in the rat kidney microsomes. However, the participation of other enzymes cannot be ruled out, since the inactivating activity of the rat kidney microsome fraction is also inhibited by 1 X 10(-4) M diisopropylfuorophosphate. | Inactivation of porcine calcitonin by rat kidney microsome. Biological activity of porcine calcitonin was most actively inactivated by the rat kidney homogenate than by other tissue homogenates. Among the various subcellular fractions of the rat kidney homogenate examined, microsome fraction was most active in the in vitro inactivation of porcine calcitonin. Inactivation of porcine calcitonin by the rat kidney microsome was dependent on pH and temperature. Inactivating activity of the rat kidney microsome was inhibited by 1 X 10(-3) M monoiodoacetate and 1 X10(-5) M p-chloromercuribenzoate. These results suggest that porcine calcitonin is probably inactivated by a SH-enzyme in the rat kidney microsomes. However, the participation of other enzymes cannot be ruled out, since the inactivating activity of the rat kidney microsome fraction is also inhibited by 1 X 10(-4) M diisopropylfuorophosphate. | [
-0.039335403591394424,
0.007565862964838743,
-0.030977096408605576,
-0.030393490567803383,
-0.029392670840024948,
-0.00004232026185491122,
0.0758989080786705,
0.06586745381355286,
-0.0016906376695260406,
0.01629509963095188,
0.004307410679757595,
-0.05841454491019249,
0.028682002797722816,
0.058235298842191696,
-0.0035270045045763254,
-0.01604762114584446,
0.021891620010137558,
0.028006747364997864,
-0.041467368602752686,
-0.0180415790528059,
0.03527463227510452,
-0.005919165443629026,
0.03290329873561859,
0.02565065026283264,
0.0601467564702034,
0.012906106188893318,
0.004993997048586607,
0.008874433115124702,
-0.0641712099313736,
-0.19341017305850983,
-0.0022163386456668377,
-0.01681859977543354,
0.04729809612035751,
-0.05263792350888252,
0.015682436525821686,
-0.010343986563384533,
0.004021117463707924,
-0.029703030362725258,
-0.03205956518650055,
0.0661667212843895,
0.0698101818561554,
0.044292304664850235,
0.05642171576619148,
0.01997416652739048,
0.007840837351977825,
0.05746086314320564,
-0.029991665855050087,
0.000052380954002728686,
0.13082870841026306,
0.034721001982688904,
0.016010459512472153,
0.005337319802492857,
0.033092767000198364,
0.045894745737314224,
0.007975849322974682,
0.004924037028104067,
0.051212698221206665,
0.039920587092638016,
0.02271282859146595,
0.06787651777267456,
0.02403993532061577,
0.059090469032526016,
-0.13148806989192963,
0.062384434044361115,
0.059600453823804855,
-0.007405071519315243,
-0.027634844183921814,
-0.062623992562294,
0.014415751211345196,
0.01668037287890911,
-0.05472646653652191,
0.05458242446184158,
0.020297393202781677,
0.016738012433052063,
0.019482582807540894,
-0.02970675379037857,
0.011738947592675686,
0.028376465663313866,
0.04568949341773987,
0.037067413330078125,
0.009814144112169743,
-0.02384321577847004,
0.04112444818019867,
-0.05070364847779274,
0.020507045090198517,
-0.03973760828375816,
-0.035401150584220886,
0.005834557116031647,
-0.013392738997936249,
-0.025806482881307602,
-0.006105308886617422,
-0.014854767359793186,
-0.004460281692445278,
-0.053313903510570526,
-0.09561409056186676,
0.056435175240039825,
0.06928485631942749,
-0.014987783506512642,
-0.041037172079086304,
0.3516857922077179,
-0.04133186861872673,
0.003577563911676407,
-0.09948412328958511,
0.013092824257910252,
0.037543922662734985,
0.06723607331514359,
-0.04127546772360802,
0.03884267807006836,
-0.000778340850956738,
0.033767759799957275,
0.02956007793545723,
0.04282904416322708,
0.013483627699315548,
-0.05818893387913704,
-0.0010625113500282168,
0.0007848812383599579,
0.06926537305116653,
0.040030885487794876,
-0.03235717490315437,
0.045625898987054825,
0.03910142928361893,
-0.031004318967461586,
-0.02283489890396595,
-0.034070584923028946,
0.03234429284930229,
-0.005972025915980339,
0.019614068791270256,
0.03586004301905632,
0.03744449093937874,
0.048522405326366425,
0.036211542785167694,
0.009090147912502289,
0.02873764932155609,
-0.029181411489844322,
0.033072520047426224,
-0.06484300643205643,
0.03370609134435654,
-0.08529482036828995,
-0.04688341170549393,
-0.05210769549012184,
-0.03692739084362984,
-0.04886823892593384,
-0.046788379549980164,
-0.08648309856653214,
-0.032560545951128006,
0.07997295260429382,
0.022404195740818977,
0.05259037762880325,
0.04134087637066841,
-0.015068775042891502,
-0.040130410343408585,
0.056000079959630966,
-0.005416134838014841,
0.0413481667637825,
-0.04707157984375954,
0.044475700706243515,
-0.008339174091815948,
-0.01753794588148594,
-0.010348460637032986,
-0.015265184454619884,
-0.127869114279747,
-0.06857006251811981,
-0.046613845974206924,
0.07893519848585129,
0.007323772646486759,
0.054620448499917984,
-0.032736361026763916,
0.019629552960395813,
0.018020370975136757,
-0.028040144592523575,
-0.021349262446165085,
0.0284817386418581,
-0.015332876704633236,
-0.09120534360408783,
-0.0323176309466362,
-0.0003253429022151977,
0.03476890176534653,
-0.038063205778598785,
0.03112683817744255,
-0.02148878201842308,
0.00560422521084547,
-0.037184640765190125,
0.0038025679532438517,
0.006280654110014439,
0.04309200868010521,
-0.03310554847121239,
-0.0482451394200325,
0.0026445065159350634,
-0.006377593148499727,
0.09108611941337585,
-0.023630423471331596,
-0.026623843237757683,
-0.02579731121659279,
-0.004244229756295681,
-0.02332305535674095,
-0.021583613008260727,
-0.016638895496726036,
0.009485569782555103,
0.010863138362765312,
-0.042305976152420044,
0.09324053674936295,
-0.031866781413555145,
0.044440727680921555,
0.030282044783234596,
-0.05610751360654831,
0.05075368657708168,
0.028751110658049583,
0.037355732172727585,
0.04147046431899071,
-0.012180325575172901,
-0.06177990511059761,
0.002493788255378604,
-0.057815343141555786,
-0.02665501832962036,
-0.02530827187001705,
-0.024072930216789246,
0.0033206657972186804,
-0.01887376978993416,
0.06755118072032928,
0.05209663137793541,
-0.0021923694293946028,
-0.039332155138254166,
-0.06256765127182007,
-0.3031172454357147,
-0.02949356473982334,
0.044290587306022644,
-0.04663335904479027,
0.09858930855989456,
-0.08842409402132034,
0.003618452465161681,
-0.02031238004565239,
-0.04302830994129181,
-0.0383249893784523,
0.039591819047927856,
0.06863737851381302,
-0.0578356496989727,
-0.025012535974383354,
0.06226973608136177,
0.055471356958150864,
0.011219465173780918,
-0.007299304939806461,
0.0437212698161602,
-0.02010626159608364,
0.027833634987473488,
0.010526594705879688,
-0.019112616777420044,
0.013617179356515408,
0.10164028406143188,
-0.004060830920934677,
0.09970884025096893,
0.03320206701755524,
-0.01614287495613098,
-0.027304569259285927,
0.005795387551188469,
-0.0024661323986947536,
-0.04860041290521622,
-0.07861174643039703,
-0.05518040060997009,
0.004326476249843836,
-0.010119841434061527,
-0.03850467875599861,
0.018137650564312935,
0.0029829584527760744,
-0.07906654477119446,
0.00007591841858811677,
-0.0547393299639225,
-0.08150279521942139,
-0.01148277334868908,
-0.020726367831230164,
0.0005586400511674583,
0.03949930518865585,
-0.016828466206789017,
-0.04877481237053871,
0.02159455604851246,
-0.03464245796203613,
0.011529353447258472,
0.03284179046750069,
0.01809258759021759,
-0.003234187141060829,
-0.03184124454855919,
0.00013379535812418908,
-0.02206740528345108,
-0.006195924244821072,
0.01733856461942196,
-0.003910723142325878,
-0.00462811766192317,
-0.016849083825945854,
0.016260504722595215,
-0.02006133832037449,
-0.0348784513771534,
0.0045398324728012085,
0.04015033692121506,
0.03593731299042702,
0.012302183546125889,
0.12097690999507904,
0.039152149111032486,
0.04943164438009262,
0.04266887903213501,
0.056839436292648315,
-0.019863994792103767,
-0.030482934787869453,
-0.03369642049074173,
0.062384169548749924,
-0.04413464292883873,
-0.05185144394636154,
0.042439188808202744,
-0.02076769806444645,
-0.01776445470750332,
0.014357849024236202,
0.01734822429716587,
0.01653340272605419,
0.12423945963382721,
0.04698418825864792,
-0.0027122816536575556,
0.0131189851090312,
-0.08597176522016525,
-0.014606127515435219,
0.06496711075305939,
-0.016486292704939842,
-0.23617441952228546,
-0.0002652113907970488,
0.02870890311896801,
-0.004133268259465694,
-0.0032194857485592365,
-0.007538237143307924,
0.007239246740937233,
-0.0453936792910099,
-0.05366373807191849,
0.025058487430214882,
0.025555891916155815,
0.04511042311787605,
0.1115555614233017,
0.012466290965676308,
-0.058089349418878555,
0.053664445877075195,
-0.023111917078495026,
-0.004297158680856228,
0.0189809612929821,
-0.02340855821967125,
0.004051252733916044,
-0.06814557313919067,
0.11693268269300461,
-0.013085207901895046,
0.009440455585718155,
0.039630044251680374,
-0.008602578192949295,
0.01171904057264328,
-0.044301070272922516,
-0.029928885400295258,
-0.03980173170566559,
0.005951972678303719,
0.04606989771127701,
-0.03901044279336929,
0.025316566228866577,
-0.02225462906062603,
-0.02959764562547207,
-0.0020759901963174343,
-0.04151910915970802,
-0.02239236794412136,
-0.03457735478878021,
0.000749646860640496,
-0.08388733863830566,
-0.0699685662984848,
0.08803275227546692,
-0.07235419005155563,
-0.07896395027637482,
-0.029840145260095596,
0.057654969394207,
0.010866580531001091,
0.00649416446685791,
-0.05260130390524864,
0.02145061269402504,
0.027255121618509293,
-0.0008639678126201034,
-0.035055045038461685,
-0.013852037489414215,
0.002620733343064785,
-0.022197289392352104,
0.005788485053926706,
-0.009042979218065739,
0.03969505429267883,
-0.033155761659145355,
0.06802734732627869,
0.039182063192129135
] |
PMID:8307 | Estimation of testosterone binding capacity in the serum with hydrophobie resin. | A method for the estimation of the serum testosterone binding capacity (TeBC) using hydrophobic resin, Amberlite XAD-2, was developed. The serum was incubated with a saturating amount of testosterone-1,2(-3)H at 15 degrees C, unbound testosterone was adsorbed to the resin and the 3H-radoiactivity remaining in the supernatant fluid was counted. Under these conditions, the normal levels and their standard deviations were 15.08+/-3.39 ng/ml (n=7) for male and 35.06+/-3.56 ng/ml (n=6) for female respectively. Precision of the method was 6.29%. TeBC in the third month of pregnancy was approximately 1.5 times more than that of non-pregnant women, and approximately 3 to 5 times in the tenth month. | Estimation of testosterone binding capacity in the serum with hydrophobie resin. A method for the estimation of the serum testosterone binding capacity (TeBC) using hydrophobic resin, Amberlite XAD-2, was developed. The serum was incubated with a saturating amount of testosterone-1,2(-3)H at 15 degrees C, unbound testosterone was adsorbed to the resin and the 3H-radoiactivity remaining in the supernatant fluid was counted. Under these conditions, the normal levels and their standard deviations were 15.08+/-3.39 ng/ml (n=7) for male and 35.06+/-3.56 ng/ml (n=6) for female respectively. Precision of the method was 6.29%. TeBC in the third month of pregnancy was approximately 1.5 times more than that of non-pregnant women, and approximately 3 to 5 times in the tenth month. | [
-0.004002485889941454,
0.08823663741350174,
0.008155452087521553,
-0.047082941979169846,
-0.023572562262415886,
-0.008104518055915833,
0.063298799097538,
0.052463825792074203,
-0.051290132105350494,
0.0036424456629902124,
-0.003483382286503911,
-0.09192105382680893,
0.031435102224349976,
0.01869162730872631,
-0.039379168301820755,
0.009221876971423626,
-0.006456793751567602,
-0.021908292546868324,
-0.01578264869749546,
0.07661200314760208,
0.04295498505234718,
0.03368474915623665,
-0.037604086101055145,
-0.049122393131256104,
0.06144176796078682,
0.033475153148174286,
-0.06425989419221878,
-0.023749569430947304,
-0.01832742989063263,
-0.17362377047538757,
0.01420681644231081,
-0.024129405617713928,
0.026384560391306877,
-0.011978167109191418,
-0.01308186911046505,
-0.0065280538983643055,
-0.014051219448447227,
-0.046631306409835815,
-0.02095719613134861,
0.010153945535421371,
-0.02933211624622345,
0.03527316451072693,
-0.016872940585017204,
0.011030771769583225,
0.02199445106089115,
0.012327540665864944,
-0.05509438365697861,
0.05401042476296425,
0.04960763081908226,
0.06631661206483841,
0.022461488842964172,
0.010154258459806442,
0.06372286379337311,
0.08409295231103897,
-0.010052294470369816,
-0.004172959830611944,
0.016173772513866425,
-0.028566742315888405,
0.030532415956258774,
0.03904532268643379,
-0.036580707877874374,
0.05074723809957504,
-0.10658849030733109,
0.06555294245481491,
0.040856704115867615,
0.026257390156388283,
-0.042926888912916183,
-0.00717663299292326,
0.057474277913570404,
-0.0010641560656949878,
0.030038585886359215,
0.03205369412899017,
0.0023943732958287,
0.012208574451506138,
0.009927486069500446,
-0.0060395654290914536,
-0.017866283655166626,
-0.03479507192969322,
0.05423672869801521,
-0.016922468319535255,
-0.04721829295158386,
0.004182192496955395,
0.03569812327623367,
0.012796147726476192,
0.02048305980861187,
-0.05142499879002571,
0.03307750076055527,
-0.0010283768642693758,
0.0208042673766613,
0.05873655527830124,
-0.023497486487030983,
-0.031449202448129654,
-0.05550786480307579,
0.006015358027070761,
-0.09064903110265732,
0.044407520443201065,
0.01705339178442955,
-0.011042098514735699,
0.01379429455846548,
0.3967106342315674,
-0.0408446229994297,
0.007739002350717783,
-0.06915703415870667,
0.0038269010838121176,
-0.013846905902028084,
-0.020764147862792015,
-0.033473044633865356,
-0.053142156451940536,
-0.06395157426595688,
0.01723407208919525,
0.010701715014874935,
-0.03833163157105446,
0.06864460557699203,
-0.07908950001001358,
-0.05158120021224022,
0.02339017391204834,
-0.0032349429093301296,
-0.003749795025214553,
-0.024343907833099365,
-0.00468785036355257,
-0.04878697171807289,
0.0035589803010225296,
0.0535859651863575,
-0.03479047119617462,
0.029233429580926895,
0.0013852983247488737,
0.029206369072198868,
0.06779152154922485,
0.0002871612086892128,
0.034687407314777374,
0.015988647937774658,
0.04331054538488388,
-0.04196113720536232,
0.0616336353123188,
0.019028034061193466,
0.0031079561449587345,
-0.0010610629105940461,
-0.05382823571562767,
-0.003816875396296382,
0.03595474734902382,
0.008365861140191555,
-0.034720927476882935,
-0.05554722622036934,
-0.01694382168352604,
-0.025657808408141136,
0.08305493742227554,
-0.018079493194818497,
-0.027868088334798813,
0.011088281869888306,
-0.039105743169784546,
0.01123106386512518,
0.10581529140472412,
-0.022378385066986084,
0.011232498101890087,
0.012405150569975376,
-0.03181855380535126,
0.000007773327524773777,
0.020714852958917618,
-0.04173245280981064,
-0.05054619163274765,
-0.03988810256123543,
-0.012191187590360641,
-0.038879480212926865,
-0.0013048050459474325,
0.0394250750541687,
-0.048116303980350494,
-0.029234839603304863,
-0.012758756056427956,
-0.014377756975591183,
-0.02565390057861805,
0.06433063745498657,
-0.04955951124429703,
-0.031334567815065384,
0.02371014840900898,
-0.008082973770797253,
-0.03277992829680443,
-0.02062872238457203,
0.001929272897541523,
0.03879290446639061,
0.0370592437684536,
0.02343887649476528,
-0.0008900658576749265,
-0.04380972683429718,
0.02353297360241413,
0.0004939584177918732,
-0.022256672382354736,
-0.011904420331120491,
-0.003740718588232994,
0.03754085674881935,
0.06811670958995819,
0.02256666123867035,
0.018495213240385056,
-0.04202509671449661,
0.03153882920742035,
0.020953761413693428,
0.018493976444005966,
-0.03872329741716385,
0.0025013647973537445,
0.019567808136343956,
-0.023651298135519028,
0.12143033742904663,
-0.04700244963169098,
0.04071081429719925,
0.02491370588541031,
0.021727146580815315,
0.08723320066928864,
0.04144434630870819,
-0.010618363507091999,
0.057173557579517365,
0.010456280782818794,
-0.05069580674171448,
0.013103996403515339,
-0.027460357174277306,
0.0054136281833052635,
-0.03412484750151634,
0.04391327127814293,
0.06727642565965652,
-0.0344652384519577,
0.019299130886793137,
0.008900269865989685,
0.051198262721300125,
-0.04342376068234444,
0.025476913899183273,
-0.3296930491924286,
0.0013077919138595462,
0.0259360671043396,
0.059844549745321274,
0.06801915168762207,
-0.09562351554632187,
-0.0006040575099177659,
-0.006455020513385534,
0.023169515654444695,
-0.04078787937760353,
0.09238844364881516,
0.11533976346254349,
-0.029952634125947952,
-0.06334782391786575,
-0.04023148864507675,
-0.010874150320887566,
0.03351438045501709,
-0.04364600405097008,
0.02659131959080696,
-0.00510206026956439,
-0.06627533584833145,
-0.016043052077293396,
-0.06457549333572388,
0.0620303638279438,
0.06962483376264572,
-0.028648441657423973,
0.08785366266965866,
-0.08181466907262802,
-0.06756392121315002,
-0.02208060212433338,
0.03942772373557091,
0.0032327475491911173,
0.0182004664093256,
-0.05274926498532295,
0.0066496520303189754,
0.006188650149852037,
-0.06114327162504196,
-0.0032168165780603886,
-0.0004257754480931908,
-0.005991714540868998,
-0.06475920230150223,
0.015318243764340878,
0.020185712724924088,
-0.040085628628730774,
-0.06182154640555382,
-0.01039437111467123,
-0.019533131271600723,
0.0035919456277042627,
-0.024752873927354813,
-0.034779515117406845,
0.07547929137945175,
-0.00884550716727972,
-0.009025119245052338,
-0.009809168055653572,
-0.00542281661182642,
0.014413238503038883,
-0.06456191092729568,
0.008398103527724743,
-0.012911530211567879,
-0.029068199917674065,
0.054642125964164734,
-0.013487499207258224,
0.05395083501935005,
-0.01882322132587433,
-0.03955235332250595,
-0.017739832401275635,
0.042859602719545364,
-0.05945277959108353,
0.04491109400987625,
0.013531679287552834,
0.009029190056025982,
0.06705217808485031,
-0.006702801212668419,
0.0795264020562172,
0.015110130421817303,
0.018530627712607384,
-0.020851951092481613,
-0.05483279377222061,
-0.040976203978061676,
-0.03113733045756817,
0.023495221510529518,
-0.0028263493441045284,
0.07896749675273895,
-0.0010923744412139058,
0.0033204294741153717,
0.01360831968486309,
0.022681547328829765,
-0.003993048332631588,
0.03879988193511963,
0.0582410991191864,
0.01817193254828453,
-0.029533037915825844,
-0.021681513637304306,
-0.012294772081077099,
0.00558512145653367,
0.015085146762430668,
-0.3250717520713806,
-0.004334871657192707,
0.005591652821749449,
0.0057824477553367615,
-0.005983160343021154,
-0.007632626686245203,
0.024896768853068352,
-0.022989699617028236,
-0.03191450983285904,
0.02341717854142189,
-0.013512876816093922,
0.024507472291588783,
0.03577117994427681,
0.017739981412887573,
-0.031802915036678314,
-0.013956320472061634,
0.03870987519621849,
-0.012984011322259903,
-0.04078526794910431,
-0.06162109598517418,
0.036505259573459625,
-0.03683903440833092,
0.14906951785087585,
0.08396151661872864,
0.009703165851533413,
0.016394363716244698,
-0.021421147510409355,
-0.017959028482437134,
-0.012009772472083569,
-0.03410530835390091,
-0.024015985429286957,
0.01061663031578064,
0.033025842159986496,
-0.04984306916594505,
-0.019262760877609253,
0.023497875779867172,
-0.016564592719078064,
-0.05614267662167549,
0.03799869492650032,
0.023450762033462524,
0.042590707540512085,
0.01889493130147457,
-0.05157966539263725,
0.01648823916912079,
0.05273065343499184,
-0.05189286544919014,
-0.04392862319946289,
0.005058306269347668,
0.03788025677204132,
-0.020255576819181442,
-0.023024849593639374,
0.035633958876132965,
0.0005374284228309989,
0.011614774353802204,
0.0710340291261673,
-0.0025766321923583746,
-0.011920257471501827,
-0.025636816397309303,
-0.032130006700754166,
-0.014764206483960152,
-0.047304220497608185,
-0.000995403970591724,
-0.046323511749506,
0.07974209636449814,
0.05081724002957344
] |
PMID:8308 | Dynamic asymmetries in liver tyrosine aminotransferase induction. | Changes in liver tyrosine aminotransferase activity were measured in mice and rats following a whole body X-irradiation in order to ascertain the dose-effect relationship. The response was either (1) linear in case of low pulses (25 rad/min); (2) parabolic, indicating the onset of a saturation process in case of medium pulses (60 rad/min), or (3) biphasic, suggesting the cooperation of at least two regulatory mechanisms following longtime exposure to strong stimuli (100 rad/min). | Dynamic asymmetries in liver tyrosine aminotransferase induction. Changes in liver tyrosine aminotransferase activity were measured in mice and rats following a whole body X-irradiation in order to ascertain the dose-effect relationship. The response was either (1) linear in case of low pulses (25 rad/min); (2) parabolic, indicating the onset of a saturation process in case of medium pulses (60 rad/min), or (3) biphasic, suggesting the cooperation of at least two regulatory mechanisms following longtime exposure to strong stimuli (100 rad/min). | [
-0.048045769333839417,
0.027322016656398773,
-0.023002978414297104,
0.01599908247590065,
-0.02720550075173378,
0.015688320621848106,
0.081779845058918,
-0.002057269448414445,
-0.018949436023831367,
-0.040653590112924576,
0.046490706503391266,
-0.06352269649505615,
0.014555582776665688,
0.05963326245546341,
-0.02304837480187416,
0.010439983569085598,
0.0016881695482879877,
-0.027172375470399857,
-0.06396999955177307,
-0.03313564136624336,
-0.037421826273202896,
0.006680712569504976,
0.07367416471242905,
-0.016528937965631485,
0.006063553970307112,
0.04401403293013573,
0.00005815716212964617,
-0.02586044743657112,
-0.03210439160466194,
-0.2065989226102829,
0.02983490750193596,
-0.04518431797623634,
-0.017216382548213005,
-0.08855316042900085,
-0.017841413617134094,
0.0016179283848032355,
-0.07515152543783188,
-0.014077842235565186,
-0.00017589404887985438,
0.00004631327465176582,
0.02575843222439289,
0.07290281355381012,
-0.0007996759959496558,
-0.030466560274362564,
-0.003701989073306322,
0.01899106614291668,
-0.03765149414539337,
-0.03778185695409775,
0.054682739078998566,
-0.027260130271315575,
-0.015892360359430313,
-0.022804109379649162,
0.07262783497571945,
0.0629027709364891,
-0.02354864589869976,
0.023811765015125275,
0.0214438084512949,
0.07179445773363113,
0.008480167016386986,
0.05264599993824959,
-0.04210759326815605,
0.016231916844844818,
-0.10360941290855408,
0.08052878826856613,
0.10385440289974213,
0.020162997767329216,
-0.026309480890631676,
-0.03834693133831024,
0.03901670128107071,
0.04273476451635361,
-0.05642714723944664,
0.017623350024223328,
-0.0042154667899012566,
-0.00382333155721426,
0.02922414429485798,
-0.035289715975522995,
-0.02136063762009144,
-0.006031581200659275,
0.07438092678785324,
-0.015413612127304077,
0.027257809415459633,
-0.0004518183704931289,
-0.010475690476596355,
0.024005640298128128,
0.012855888344347477,
-0.0033483391162008047,
-0.0017413902096450329,
-0.028502997010946274,
-0.017890382558107376,
0.04977943375706673,
-0.06631133705377579,
-0.02694757655262947,
0.03289807215332985,
-0.02924860082566738,
-0.08037103712558746,
0.02163510024547577,
0.04043855518102646,
0.01696258969604969,
0.058807749301195145,
0.398658812046051,
0.029408056288957596,
0.04867757111787796,
-0.11179602146148682,
0.02699269913136959,
-0.035846203565597534,
-0.011985745280981064,
0.014179845340549946,
0.009082014672458172,
0.0075767990201711655,
0.0062728095799684525,
0.046853724867105484,
0.003232811111956835,
0.02864069864153862,
-0.03303862735629082,
-0.04549688845872879,
0.050753816962242126,
0.02637752890586853,
0.01887349784374237,
0.017405696213245392,
0.012740991078317165,
-0.01836850494146347,
0.02746829204261303,
0.009594012051820755,
-0.03230670094490051,
0.02305329032242298,
0.020131874829530716,
-0.022252516821026802,
0.07074998319149017,
0.02118160016834736,
0.010933807119727135,
0.03855234012007713,
0.01769239269196987,
0.03473867103457451,
0.007641332224011421,
0.02641311287879944,
0.02372152917087078,
-0.011046085506677628,
-0.04681973159313202,
-0.009280274622142315,
0.06499767303466797,
-0.0011943411082029343,
-0.036325834691524506,
-0.005158226937055588,
-0.06586798280477524,
-0.04851454496383667,
0.09561667591333389,
0.032914143055677414,
0.02321167103946209,
0.004537475761026144,
-0.02681772969663143,
-0.01029282622039318,
0.017560241743922234,
-0.018736550584435463,
0.01366440486162901,
-0.051685772836208344,
0.036775339394807816,
-0.036131978034973145,
-0.004080105572938919,
0.010922746732831001,
-0.0505734421312809,
-0.04850653558969498,
-0.020375292748212814,
-0.06304657459259033,
0.08974894881248474,
0.009668800048530102,
0.03316814452409744,
-0.06822981685400009,
0.047645967453718185,
0.015210147015750408,
-0.02721712924540043,
-0.028689920902252197,
0.00019988245912827551,
-0.014382013119757175,
-0.028149500489234924,
-0.019376831129193306,
-0.03389333561062813,
0.03967388719320297,
0.019733872264623642,
-0.0032630139030516148,
0.007139613851904869,
0.061707302927970886,
-0.08387186378240585,
0.004634338431060314,
0.0846935510635376,
0.0115309813991189,
0.0072091748006641865,
-0.011636560782790184,
0.030746961012482643,
0.029957767575979233,
0.034973617643117905,
-0.014141869731247425,
-0.028685981407761574,
-0.08220445364713669,
0.005368831101804972,
-0.020341644063591957,
-0.012001764960587025,
0.01435555424541235,
0.05429934337735176,
-0.003618406830355525,
-0.03135617449879646,
0.049980223178863525,
0.026079826056957245,
0.0414256751537323,
0.07749219238758087,
-0.0070064738392829895,
-0.014541813172399998,
-0.006254555191844702,
0.009394388645887375,
0.039998527616262436,
-0.01716996356844902,
-0.03990155830979347,
0.004322526976466179,
-0.03208504989743233,
-0.002331502502784133,
-0.05908624082803726,
0.03645016998052597,
0.035533297806978226,
0.006450834218412638,
0.03624054789543152,
0.018722951412200928,
0.027006860822439194,
-0.0945916548371315,
-0.008139406330883503,
-0.3253774642944336,
-0.007895191200077534,
0.004759136587381363,
0.014246385544538498,
0.024313999339938164,
-0.059211984276771545,
-0.012572309002280235,
-0.03271599858999252,
0.05535273626446724,
0.04280947893857956,
0.07658351957798004,
0.08048683404922485,
0.013830583542585373,
-0.10602863132953644,
0.011556537821888924,
-0.0025070179253816605,
-0.014383494853973389,
0.015384424477815628,
0.03573460131883621,
0.010160264559090137,
0.0019291018834337592,
0.0010516728507354856,
-0.008400313556194305,
-0.06453049182891846,
0.014511756598949432,
-0.0059377457946538925,
0.08822216093540192,
0.020481104031205177,
-0.017641516402363777,
-0.0036244613584131002,
-0.025563132017850876,
0.00835665687918663,
0.0027526728808879852,
-0.06961958855390549,
0.00036248023388907313,
-0.024183010682463646,
0.0022061793133616447,
-0.027951069176197052,
-0.028800513595342636,
-0.013907887041568756,
-0.06082184612751007,
-0.04279831796884537,
0.000623541884124279,
-0.05614817515015602,
-0.026680372655391693,
-0.03760306537151337,
0.04414980113506317,
0.026542719453573227,
0.009421244263648987,
-0.05030457302927971,
0.04658406972885132,
-0.04876483231782913,
-0.02544412575662136,
0.021609384566545486,
-0.0024522761814296246,
-0.07503758370876312,
-0.03474757820367813,
-0.02065340243279934,
-0.017231162637472153,
-0.016596652567386627,
0.0025342984590679407,
0.045703914016485214,
-0.023928698152303696,
0.02557036280632019,
-0.029352402314543724,
0.012676817364990711,
-0.018754879012703896,
-0.02545798383653164,
0.01356083806604147,
-0.004190450068563223,
0.029907289892435074,
0.12626098096370697,
-0.03481646999716759,
0.03324691951274872,
0.018909353762865067,
-0.0028507374227046967,
-0.04633662849664688,
-0.01618555374443531,
-0.112002894282341,
0.04416878893971443,
-0.012311175465583801,
-0.019379248842597008,
0.005896607413887978,
0.03022802248597145,
-0.001996133243665099,
0.02559041976928711,
0.020044876262545586,
-0.032907627522945404,
0.009753582999110222,
0.003511324990540743,
0.025271495804190636,
0.01907993108034134,
-0.027433712035417557,
-0.022186854854226112,
0.011006610468029976,
0.018994446843862534,
-0.30030718445777893,
0.024257274344563484,
-0.0049031926319003105,
-0.010027878917753696,
-0.05514250323176384,
0.017334843054413795,
0.002495944732800126,
-0.04355127736926079,
-0.08334381133317947,
0.006179751828312874,
-0.005543224513530731,
0.01631741039454937,
0.10411820560693741,
0.11106236279010773,
-0.007357756607234478,
0.004367533605545759,
0.0445202998816967,
-0.01566755212843418,
-0.006176698952913284,
-0.06342142075300217,
0.011178947053849697,
-0.03745284676551819,
0.17244230210781097,
-0.026419784873723984,
0.0002227997174486518,
0.06630194187164307,
-0.005561371333897114,
0.0468548908829689,
-0.0013133921893313527,
0.02326776646077633,
-0.00561223179101944,
0.039783596992492676,
0.06727135926485062,
-0.015527671203017235,
0.034868571907281876,
0.0840878039598465,
-0.026085002347826958,
0.06309168040752411,
-0.04938006401062012,
0.0005282688653096557,
0.016889411956071854,
0.007893488742411137,
-0.006285732612013817,
-0.04092204570770264,
0.05792107433080673,
-0.05168730765581131,
-0.0393311083316803,
-0.054977379739284515,
0.018071409314870834,
0.03489212319254875,
-0.04314836487174034,
0.004657937213778496,
0.002823409391567111,
0.009209273383021355,
0.03650843724608421,
-0.004996604286134243,
-0.03467739745974541,
-0.02108365297317505,
-0.007334105204790831,
-0.007792954798787832,
-0.016620811074972153,
0.023964710533618927,
-0.010436991229653358,
0.06184970214962959,
0.03892171382904053
] |
PMID:8309 | Amino acid metabolizing enzymes in rat submaxillary gland, normal or neoplastic, and in pancreas. | The activities of 12 enzymes, many related to ornithine metabolism, were measured in rat submaxillary gland, submaxillary gland tumors and pancreas. In submaxillary gland, the activities of arginase, ornithine aminotransferase, pyrroline-5-carboxylate reductase and glutamine synthetase were high, but no ornithine transcarbamylase or proline oxidase could be detected. In the fetal submaxillary gland, arginase was at almost adult levels while ornithine aminotransferase reached 50% of its adult value postnatally. Submaxillary tumors deviated from their cognate tissue by lower levels of amino acid metabolizing enzymes and by high concentrations of thymidine kinase. In pancreas, none of the pyrroline-5-carboxylate metabolizing enzymes were as high as in either liver or submaxillary gland. The outstanding activities were those of gamma-glutamyl transpeptidase and glutamate dehydrogenase. Although arginase activities in submaxillary gland and pancreas were quantitatively similar, they differed qualitatively: submaxillary gland contained the same variant as liver while the pancreatic isozymes resembled those of other nonhepatic tissues. | Amino acid metabolizing enzymes in rat submaxillary gland, normal or neoplastic, and in pancreas. The activities of 12 enzymes, many related to ornithine metabolism, were measured in rat submaxillary gland, submaxillary gland tumors and pancreas. In submaxillary gland, the activities of arginase, ornithine aminotransferase, pyrroline-5-carboxylate reductase and glutamine synthetase were high, but no ornithine transcarbamylase or proline oxidase could be detected. In the fetal submaxillary gland, arginase was at almost adult levels while ornithine aminotransferase reached 50% of its adult value postnatally. Submaxillary tumors deviated from their cognate tissue by lower levels of amino acid metabolizing enzymes and by high concentrations of thymidine kinase. In pancreas, none of the pyrroline-5-carboxylate metabolizing enzymes were as high as in either liver or submaxillary gland. The outstanding activities were those of gamma-glutamyl transpeptidase and glutamate dehydrogenase. Although arginase activities in submaxillary gland and pancreas were quantitatively similar, they differed qualitatively: submaxillary gland contained the same variant as liver while the pancreatic isozymes resembled those of other nonhepatic tissues. | [
-0.007010385859757662,
-0.040037523955106735,
-0.03225761651992798,
-0.01906086876988411,
-0.01278732344508171,
-0.015245155431330204,
0.0659647136926651,
0.01832496002316475,
-0.044385120272636414,
-0.007756675127893686,
0.02488766983151436,
-0.07500997930765152,
0.03126337379217148,
0.008127674460411072,
-0.0031082024797797203,
-0.03873388469219208,
0.018787717446684837,
0.0085248202085495,
-0.0028083468787372112,
-0.057809747755527496,
0.04200650006532669,
0.0017594903474673629,
0.07690493762493134,
-0.0765191987156868,
0.08268268406391144,
0.04309020936489105,
-0.004635500255972147,
-0.039266977459192276,
-0.06670085340738297,
-0.1675473302602768,
0.05409757420420647,
-0.03360988199710846,
-0.008390557020902634,
0.005767119582742453,
0.005066050216555595,
0.007832616567611694,
-0.04570109024643898,
0.037732116878032684,
-0.04400528967380524,
0.05678252503275871,
-0.006264518015086651,
0.008843502029776573,
-0.011405831202864647,
0.07766050100326538,
0.023571547120809555,
0.0537063404917717,
-0.05761324614286423,
-0.03801275044679642,
0.057328883558511734,
-0.029635731130838394,
0.004925796762108803,
-0.013199723325669765,
0.04003818333148956,
0.031130336225032806,
0.049415044486522675,
-0.03603721782565117,
0.0629989504814148,
0.01919419877231121,
0.0025045049842447042,
0.027232877910137177,
-0.010824205353856087,
0.04921703413128853,
-0.11426100134849548,
0.03722622990608215,
0.0449325293302536,
-0.010353649966418743,
-0.06965288519859314,
-0.04996678978204727,
0.03406635671854019,
0.019355926662683487,
-0.08280133455991745,
0.0038917253259569407,
-0.010941583663225174,
-0.005605394020676613,
0.03482088819146156,
-0.02475818060338497,
0.03147824481129646,
0.02930760197341442,
0.02857901155948639,
0.05397411808371544,
0.005842830054461956,
-0.014263155870139599,
0.015351228415966034,
-0.006850922014564276,
0.012427403591573238,
-0.012097571976482868,
-0.02827049233019352,
-0.056110914796590805,
0.03125330060720444,
0.0006631530122831464,
-0.017691681161522865,
-0.04949115589261055,
-0.013165974989533424,
-0.049140892922878265,
-0.049069881439208984,
0.006302687339484692,
0.017228785902261734,
-0.00031475527794100344,
0.05064276605844498,
0.37142807245254517,
0.0044987015426158905,
-0.03477809205651283,
-0.06905815005302429,
0.05462690815329552,
-0.05970851331949234,
-0.032597750425338745,
-0.06778524070978165,
0.031476836651563644,
0.005652781575918198,
0.06806650757789612,
0.09120423346757889,
0.04717608541250229,
0.02159150131046772,
-0.047003794461488724,
-0.024273358285427094,
-0.00034284740104340017,
0.05402218922972679,
0.0277034193277359,
-0.07912513613700867,
0.07602110505104065,
-0.02396831288933754,
-0.04327452555298805,
-0.0010760774603113532,
-0.006531399209052324,
-0.007005385588854551,
0.04345617815852165,
-0.03291645646095276,
0.10561627894639969,
0.06000335142016411,
0.0341155081987381,
0.020907612517476082,
0.07626060396432877,
-0.03647222742438316,
-0.004622669890522957,
0.0037231005262583494,
-0.04270632565021515,
0.06506264209747314,
-0.005313447676599026,
0.006729699205607176,
-0.0031204174738377333,
-0.052639544010162354,
-0.1054605096578598,
-0.010367311537265778,
-0.041305869817733765,
-0.03620501235127449,
0.12557829916477203,
-0.03768778219819069,
-0.0025264734867960215,
0.037136927247047424,
-0.03310532495379448,
0.05211174488067627,
0.03380068391561508,
-0.0023756532464176416,
0.021604860201478004,
-0.01892232894897461,
0.06803935021162033,
-0.04745445400476456,
0.013122966513037682,
-0.00246639596298337,
-0.0038242866285145283,
-0.05674732103943825,
-0.01728683151304722,
0.021405501291155815,
0.022349754348397255,
0.05077148228883743,
0.00571216968819499,
-0.03900633752346039,
-0.04550279676914215,
-0.013942456804215908,
0.05227219685912132,
0.022837871685624123,
0.016929125413298607,
-0.03646989166736603,
0.020391182973980904,
-0.0413268506526947,
0.030623851343989372,
0.033592164516448975,
0.0475432313978672,
0.005394918378442526,
-0.013181325048208237,
0.02686489187180996,
-0.029286906123161316,
-0.011437193490564823,
0.01351026352494955,
0.0199827142059803,
0.01084316149353981,
0.010727887973189354,
-0.004344141110777855,
0.009932322427630424,
0.03619759529829025,
-0.06394264101982117,
-0.008392966352403164,
-0.10962119698524475,
-0.022310331463813782,
-0.024221183732151985,
-0.03311103582382202,
-0.018757065758109093,
0.05866605415940285,
-0.0030666221864521503,
-0.053493939340114594,
0.11971305310726166,
0.031214701011776924,
0.030823413282632828,
0.04315231367945671,
0.06679800897836685,
0.01705622300505638,
-0.012393432669341564,
-0.018347270786762238,
0.049114372581243515,
-0.01712755300104618,
-0.0495002306997776,
-0.025967910885810852,
-0.010193385183811188,
0.043882522732019424,
-0.0391043983399868,
0.05018385499715805,
0.0020767657551914454,
-0.004311058670282364,
0.06746476888656616,
0.051378555595874786,
0.0159060787409544,
-0.09884142130613327,
-0.002111340407282114,
-0.2952926456928253,
-0.06856703013181686,
0.012166352942585945,
-0.05437946319580078,
0.005053854547441006,
-0.011163294315338135,
0.014862180687487125,
-0.038393497467041016,
0.013186994008719921,
-0.003647507866844535,
0.049955762922763824,
0.02658635936677456,
-0.0004504914686549455,
-0.008066954091191292,
0.03837541863322258,
0.06932482123374939,
-0.015652796253561974,
0.001413727761246264,
0.014784004539251328,
0.019822116941213608,
0.0032242396846413612,
-0.05555950477719307,
0.0458967462182045,
-0.01619446650147438,
0.05294954031705856,
-0.028652969747781754,
0.08968830853700638,
0.012580504640936852,
0.002078079851344228,
-0.04431657865643501,
0.03195419907569885,
0.02046196721494198,
-0.06265129894018173,
-0.10088051855564117,
-0.03690545633435249,
-0.05168219283223152,
-0.029005909338593483,
-0.02826560288667679,
-0.04983384534716606,
-0.03235272690653801,
-0.06762468069791794,
-0.006460162810981274,
0.010820796713232994,
-0.07408090680837631,
-0.0294033233076334,
-0.003415971528738737,
-0.0025538494810462,
-0.00742110563442111,
0.03457524999976158,
-0.016622964292764664,
0.04287151247262955,
-0.0060293301939964294,
-0.023531045764684677,
-0.05510006844997406,
-0.014225183986127377,
-0.025708332657814026,
-0.0584050677716732,
-0.014762965962290764,
-0.07005230337381363,
0.03287218511104584,
-0.00834103673696518,
0.0024175229482352734,
-0.03896152973175049,
-0.0030921194702386856,
-0.012946250848472118,
0.0398857556283474,
0.0023117212112993,
-0.0004925329121761024,
0.011494258418679237,
0.016796549782156944,
0.037922222167253494,
0.10134249180555344,
-0.08603775501251221,
-0.00030678382609039545,
0.004157580900937319,
0.000789792975410819,
0.03184060379862785,
-0.02383432164788246,
-0.11082297563552856,
0.03388939052820206,
-0.011580689810216427,
-0.032896824181079865,
0.06993493437767029,
-0.0005675813881680369,
-0.018811816349625587,
0.044806547462940216,
0.02921016700565815,
-0.02949000895023346,
-0.004220194648951292,
0.023491403087973595,
0.08000369369983673,
0.03147146478295326,
-0.04727331921458244,
-0.03029569424688816,
0.010821716859936714,
0.02551734261214733,
-0.2658093571662903,
0.020642157644033432,
0.0038603993598371744,
-0.009421254508197308,
-0.041800279170274734,
-0.000608072557952255,
0.011979088187217712,
0.007156590931117535,
-0.008765575475990772,
0.019303439185023308,
0.06745997816324234,
0.017766844481229782,
0.07214584946632385,
0.060820236802101135,
-0.028950050473213196,
0.0064871893264353275,
0.05223436653614044,
0.01892898418009281,
0.06173134222626686,
0.0036939792335033417,
-0.030156666412949562,
-0.023596296086907387,
0.14676575362682343,
-0.004357281140983105,
-0.0021284613758325577,
0.015033954754471779,
-0.021044429391622543,
0.015444602817296982,
-0.009960454888641834,
0.02589286118745804,
-0.005046972073614597,
0.036129407584667206,
0.028618844226002693,
-0.04405916854739189,
0.04021993279457092,
0.08289884030818939,
-0.04989495873451233,
0.010761421173810959,
-0.050858043134212494,
0.02943073771893978,
-0.02326180599629879,
0.013678212650120258,
-0.07526382803916931,
-0.042097002267837524,
0.05769707262516022,
-0.10603098571300507,
-0.04475557059049606,
-0.02550552599132061,
0.0887574627995491,
-0.04969228804111481,
-0.019591843709349632,
-0.035862069576978683,
0.012136293575167656,
0.04533633962273598,
0.032467056065797806,
-0.0008987721521407366,
0.008144989609718323,
-0.005528368055820465,
0.021788321435451508,
0.042404450476169586,
-0.008530255407094955,
0.0052374182268977165,
-0.039559442549943924,
0.12985743582248688,
-0.003521478269249201
] |
PMID:8310 | Relationships of femoral venous [K+], PO2, osmolality, and [orthophosphate) with heart rate, ventilation, and leg blood flow during bicycle exercise in athletes and non-athletes. | The relationship of femoral venous [K+], [H+], osmolality (OSM), PO2, and [inorganic phosphate] ([Pi]) with heart rate (HR), ventilation (VE), and calculated leg blood flow (Q) were investigated during bicycle exercise in endurance trained (TR) and untrained (UT) test subjects. At a given VO2 the increases of [K+], OSM, [Pi] and the decrease of PO2 were significantly lower in TR than in UT. In the same proportion the increases of HR, VE, and Q were diminished. Thus in TR and UT identical and highly significantly correlated regression lines of [K+], [H+], OSM, [Pi] and PO2 with HR, VE, and Q were obtained. These constituents changed in the same proportion as the relative VO2 in TR and UT. No relationships with [Na+], [Ca++], and [ Mg++] were found. By means of a multiple regression analysis the partial influence of K+, H+, OSM, PO2, and Pi upon the total change of HR, VE and Q was estimated to compare with data from infusion experiments. The findings were discussed in view of the hypothesis that these candidates may provide linkage between metabolic events, circulatory, and ventilatory adjustments during work. | Relationships of femoral venous [K+], PO2, osmolality, and [orthophosphate) with heart rate, ventilation, and leg blood flow during bicycle exercise in athletes and non-athletes. The relationship of femoral venous [K+], [H+], osmolality (OSM), PO2, and [inorganic phosphate] ([Pi]) with heart rate (HR), ventilation (VE), and calculated leg blood flow (Q) were investigated during bicycle exercise in endurance trained (TR) and untrained (UT) test subjects. At a given VO2 the increases of [K+], OSM, [Pi] and the decrease of PO2 were significantly lower in TR than in UT. In the same proportion the increases of HR, VE, and Q were diminished. Thus in TR and UT identical and highly significantly correlated regression lines of [K+], [H+], OSM, [Pi] and PO2 with HR, VE, and Q were obtained. These constituents changed in the same proportion as the relative VO2 in TR and UT. No relationships with [Na+], [Ca++], and [ Mg++] were found. By means of a multiple regression analysis the partial influence of K+, H+, OSM, PO2, and Pi upon the total change of HR, VE and Q was estimated to compare with data from infusion experiments. The findings were discussed in view of the hypothesis that these candidates may provide linkage between metabolic events, circulatory, and ventilatory adjustments during work. | [
-0.034042783081531525,
0.03195277228951454,
0.02691681496798992,
0.013368482701480389,
0.04470376297831535,
0.020333824679255486,
0.03368847444653511,
0.05626058578491211,
-0.013965114951133728,
0.0011460889363661408,
-0.007414134684950113,
-0.0973220020532608,
0.012969698756933212,
0.008549356833100319,
0.0066800108179450035,
-0.027159590274095535,
0.04317762702703476,
0.017849158495664597,
-0.05596543475985527,
0.03558363765478134,
-0.039435144513845444,
-0.02422768995165825,
0.05497986823320389,
-0.027202975004911423,
0.03441031277179718,
0.024719079956412315,
-0.01037461869418621,
0.017059531062841415,
-0.03773023188114166,
-0.1850873827934265,
-0.0076243388466537,
0.03222455084323883,
0.0049033560790121555,
0.024387020617723465,
-0.025041822344064713,
0.04505220428109169,
-0.07159066945314407,
0.02368050627410412,
-0.04976566880941391,
0.052488092333078384,
0.018344122916460037,
-0.004549596458673477,
0.03579319268465042,
0.0493396557867527,
0.014803589321672916,
-0.0032808997202664614,
-0.0170024074614048,
0.007439691573381424,
0.07092507183551788,
0.018839754164218903,
0.02935209311544895,
0.029511213302612305,
0.030653033405542374,
0.05591185763478279,
0.030336493626236916,
-0.0450107604265213,
0.02970586158335209,
0.0309267807751894,
-0.0002384504914516583,
0.010470708832144737,
-0.011245917528867722,
0.08485416322946548,
-0.17599645256996155,
0.0262313075363636,
0.05333845689892769,
-0.024498598650097847,
-0.0671897605061531,
0.02822418510913849,
-0.00858936458826065,
0.05990066006779671,
-0.037167832255363464,
0.058231718838214874,
0.01301943976432085,
-0.00006355129153234884,
0.021750571206212044,
0.01302120927721262,
0.01686021313071251,
-0.016740115359425545,
0.02384139783680439,
0.00964419636875391,
-0.01156727597117424,
0.029760902747511864,
-0.009378028102219105,
-0.04024814814329147,
0.007693021558225155,
-0.050258200615644455,
0.01503458060324192,
-0.04744882136583328,
-0.011146563105285168,
0.017182163894176483,
-0.08714275807142258,
-0.048360761255025864,
-0.03399946913123131,
0.004859225824475288,
-0.036277592182159424,
0.05095807462930679,
0.02151460573077202,
0.02537592127919197,
-0.009533845819532871,
0.4147699773311615,
-0.017099620774388313,
0.06445178389549255,
0.020615793764591217,
0.03617868945002556,
0.06304191797971725,
-0.05687471851706505,
0.0027349800802767277,
-0.023604948073625565,
0.014522693119943142,
0.042506761848926544,
0.05954609811306,
0.012587446719408035,
0.04751360043883324,
-0.021780719980597496,
-0.04347631707787514,
0.07777392864227295,
0.0026671183295547962,
0.03540262207388878,
-0.01602235436439514,
0.03242115676403046,
-0.06271127611398697,
-0.03644881770014763,
0.02676323615014553,
-0.018438421189785004,
0.027297459542751312,
-0.04955779016017914,
-0.02013721503317356,
0.07004227489233017,
0.07635871320962906,
-0.018342049792408943,
0.0990075096487999,
0.008271696977317333,
-0.05544854700565338,
-0.04109469801187515,
-0.01968327723443508,
-0.047362975776195526,
0.03641035780310631,
-0.02732119709253311,
-0.008181221783161163,
0.04819626361131668,
-0.013963441364467144,
-0.016651680693030357,
0.05111318454146385,
-0.11803789436817169,
-0.07108622044324875,
0.10434459149837494,
0.048728689551353455,
-0.0103424908593297,
-0.04355964809656143,
-0.015855249017477036,
-0.01969827525317669,
0.03974241018295288,
-0.04113253578543663,
0.00006095492062740959,
-0.0322226844727993,
0.00460231676697731,
-0.01074332557618618,
0.019719120115041733,
-0.013604660518467426,
-0.00788617692887783,
-0.038983989506959915,
-0.07093879580497742,
-0.03521456941962242,
0.034319497644901276,
0.04000188410282135,
-0.0001362497132504359,
0.025421656668186188,
-0.042159345000982285,
0.01958583854138851,
-0.02190362475812435,
0.04751566797494888,
-0.017130063846707344,
-0.024671467021107674,
-0.05036994814872742,
0.04482433572411537,
-0.012235642410814762,
0.02121707610785961,
0.005848758853971958,
0.04196372628211975,
-0.041741326451301575,
0.05456993356347084,
0.013088710606098175,
-0.02915055863559246,
0.0015749492449685931,
0.05312681943178177,
0.008273218758404255,
-0.08605581521987915,
-0.01687786914408207,
-0.0014584424206987023,
0.09085311740636826,
0.0006697713397443295,
-0.04309802129864693,
-0.04889130964875221,
-0.025969821959733963,
-0.017064230516552925,
-0.017099928110837936,
0.00470355711877346,
0.0009588964167051017,
-0.06370991468429565,
0.012992862612009048,
0.04931987076997757,
0.010063608177006245,
0.03184226527810097,
0.008940404281020164,
-0.006100163329392672,
0.0064870030619204044,
-0.033204320818185806,
0.009994144551455975,
-0.005827459506690502,
0.001656528445892036,
-0.01205519586801529,
-0.020933203399181366,
-0.0401221439242363,
-0.018233371898531914,
0.001425241120159626,
0.0067550186067819595,
-0.007058887276798487,
0.034071143716573715,
-0.005702354945242405,
0.034661781042814255,
0.025341030210256577,
-0.03923676908016205,
-0.0018681003712117672,
-0.3286011815071106,
-0.05992095544934273,
0.027465641498565674,
0.051305729895830154,
0.07403602451086044,
0.010971687734127045,
0.013086626306176186,
0.021593088284134865,
-0.01959926448762417,
-0.025846021249890327,
0.03998551890254021,
0.01890423521399498,
-0.04457987844944,
0.0014359770575538278,
0.018207646906375885,
0.06673585623502731,
0.05816075950860977,
-0.05340006947517395,
-0.022663826122879982,
0.045282281935214996,
0.0428280308842659,
-0.001660031615756452,
0.006642638705670834,
0.039765436202287674,
-0.011022038757801056,
-0.021387159824371338,
0.0983792170882225,
-0.014920960180461407,
-0.037422772496938705,
-0.061967961490154266,
-0.024292996153235435,
0.02746802195906639,
0.015833929181098938,
-0.07486139982938766,
0.0010381201282143593,
-0.03888053074479103,
-0.011492353864014149,
0.005506379529833794,
-0.006988177541643381,
-0.04656954109668732,
-0.1067303717136383,
0.029578814283013344,
0.006013975944370031,
-0.009494025260210037,
-0.06100115552544594,
-0.018860945478081703,
-0.018297886475920677,
-0.029651176184415817,
-0.05334881693124771,
0.007581578567624092,
0.049515243619680405,
0.02148464322090149,
0.06396815180778503,
-0.049221470952034,
0.042646896094083786,
-0.06601464748382568,
-0.06972396373748779,
-0.020394830033183098,
-0.036212243139743805,
-0.06376836448907852,
-0.01606152020394802,
-0.022757122293114662,
0.0708143562078476,
-0.006627872120589018,
0.01134207658469677,
-0.030775144696235657,
0.0481591522693634,
0.021809754893183708,
0.06961491703987122,
0.013797511346638203,
0.015630628913640976,
0.07238627225160599,
0.023055339232087135,
0.0009747376898303628,
0.06444687396287918,
-0.0009607841493561864,
-0.015187172219157219,
0.02709827572107315,
0.004377758130431175,
0.02033211663365364,
0.05621324107050896,
-0.0212797112762928,
0.041370078921318054,
0.003506677458062768,
-0.01949482224881649,
0.011599034070968628,
0.01798238418996334,
0.01208913791924715,
0.01472718920558691,
0.01906099170446396,
-0.041239939630031586,
-0.020025834441184998,
0.00415847497060895,
-0.03218100965023041,
0.01800508424639702,
0.02309313602745533,
-0.29269522428512573,
-0.029002277180552483,
0.014932769350707531,
-0.016434339806437492,
-0.03223049268126488,
-0.058181021362543106,
0.02216438017785549,
-0.036166924983263016,
0.009036648087203503,
0.01854550652205944,
0.0028531579300761223,
0.056279607117176056,
0.01841013692319393,
0.049237996339797974,
-0.011835361830890179,
0.050101544708013535,
0.06855743378400803,
-0.045834459364414215,
0.011181375943124294,
-0.04727686196565628,
-0.007479014806449413,
-0.027796680107712746,
0.1246577650308609,
-0.015252726152539253,
0.0029370926786214113,
0.05248910188674927,
-0.01707284152507782,
0.028754089027643204,
-0.03817054256796837,
-0.017071738839149475,
-0.03738803043961525,
-0.012906406074762344,
0.10453449189662933,
0.0506669245660305,
-0.04558347910642624,
0.015854381024837494,
-0.028513170778751373,
0.0011380617506802082,
-0.0020844133105129004,
-0.02305370569229126,
0.04335397109389305,
-0.014119519852101803,
-0.05065278708934784,
-0.04508282616734505,
0.08988473564386368,
-0.030238185077905655,
-0.021841805428266525,
-0.054058875888586044,
0.023661794140934944,
-0.038681067526340485,
-0.026897843927145004,
-0.061025604605674744,
0.009850960224866867,
0.048868052661418915,
0.023639075458049774,
-0.0011407246347516775,
0.007813035510480404,
-0.0450909323990345,
0.016551336273550987,
-0.055963024497032166,
0.0036697702016681433,
-0.02520265430212021,
-0.05039263516664505,
0.06768561154603958,
-0.029491279274225235
] |
PMID:8311 | On the transport of tripeptide antibiotics in bacteria. | The two tripeptide antibiotics L-2-amino-4-methylphosphinobutyryl-alanyl-alanyl-alanine (L-phosphinothricyl-alanyl-alanine) and L-(N5-phosphono)methionine-S-sulfoximinyl-alanyl-alanine, both inhibitors of the glutamine synthetase, are transported into the cell of Escherichia coli K 12 via the oligopeptide transport system. The uptake by this system is proved first of all by cross-resistance with tri-L-ornithine using oligopeptide-transport-deficient mutants, and secondly by antagonism tests demonstrating competitive reversal of the action of the antibiotic by several peptides which have been shown to be transported via the oligopeptide transport system, e.g. tri-L-alanine, tetra-L-alanine, tri-L-lysine, tri-L-serine, tri-glycine, glycyl-glycyl-L-alanine and the synthetic tripeptide L-azadenyl-aminohexanoyl-alanyl-alanine. On the other hand, there is no effect on the action of the antibiotic in antagonism tests with compounds which use different transport systems, such as L-alanyl-alanine, L-lysyl-lysine, glutathione and the synthetic amino acid azaadenylaminohexanoic acid, i.e. 2-amino-6-(7-amino-3H-v-triazolo-[4,5-d]-pyrimidin-3-yl)hexanoic acid. Another inhibitor of the glutamine synthetase, L-methionine-S-dioxide (methioninesulfone) could be converted into a tripeptide form by linkage to L-alanyl-alanine analogously to the tripeptide antibiotics described above. Whereas the free L-methionine-S-dioxide seems to be transported via the methionine transport system, the tripeptide form is transported via the oligopeptide transport system. Thus, this glutamine synthetase inhibitor can be taken up by the cell via two different transport mechanisms. Our results indicate that this could provide a synergistic effect. The syntheses of the new tripeptides L-azaadenylaminohexanoyl-alanyl-alanine and L-methionine-S-dioxidyl-alanyl-alanine were performed by dicyclohexylcarbodiimide couplings of the unusual N-protected L-alpha-amino acids azaadenylaminohexanoic acid and L-methionine-S-dioxide to L-alanyl-alanine-tert-butyl ester followed by common deprotection steps. Tri-L-ornithine was synthesized without carboxyl protection via two successive couplings of hydroxybenzotriazol esters of Nalpha-butoxycarbonyl-Ndelta-benzyloxycarbonyl-L-ornithine. | On the transport of tripeptide antibiotics in bacteria. The two tripeptide antibiotics L-2-amino-4-methylphosphinobutyryl-alanyl-alanyl-alanine (L-phosphinothricyl-alanyl-alanine) and L-(N5-phosphono)methionine-S-sulfoximinyl-alanyl-alanine, both inhibitors of the glutamine synthetase, are transported into the cell of Escherichia coli K 12 via the oligopeptide transport system. The uptake by this system is proved first of all by cross-resistance with tri-L-ornithine using oligopeptide-transport-deficient mutants, and secondly by antagonism tests demonstrating competitive reversal of the action of the antibiotic by several peptides which have been shown to be transported via the oligopeptide transport system, e.g. tri-L-alanine, tetra-L-alanine, tri-L-lysine, tri-L-serine, tri-glycine, glycyl-glycyl-L-alanine and the synthetic tripeptide L-azadenyl-aminohexanoyl-alanyl-alanine. On the other hand, there is no effect on the action of the antibiotic in antagonism tests with compounds which use different transport systems, such as L-alanyl-alanine, L-lysyl-lysine, glutathione and the synthetic amino acid azaadenylaminohexanoic acid, i.e. 2-amino-6-(7-amino-3H-v-triazolo-[4,5-d]-pyrimidin-3-yl)hexanoic acid. Another inhibitor of the glutamine synthetase, L-methionine-S-dioxide (methioninesulfone) could be converted into a tripeptide form by linkage to L-alanyl-alanine analogously to the tripeptide antibiotics described above. Whereas the free L-methionine-S-dioxide seems to be transported via the methionine transport system, the tripeptide form is transported via the oligopeptide transport system. Thus, this glutamine synthetase inhibitor can be taken up by the cell via two different transport mechanisms. Our results indicate that this could provide a synergistic effect. The syntheses of the new tripeptides L-azaadenylaminohexanoyl-alanyl-alanine and L-methionine-S-dioxidyl-alanyl-alanine were performed by dicyclohexylcarbodiimide couplings of the unusual N-protected L-alpha-amino acids azaadenylaminohexanoic acid and L-methionine-S-dioxide to L-alanyl-alanine-tert-butyl ester followed by common deprotection steps. Tri-L-ornithine was synthesized without carboxyl protection via two successive couplings of hydroxybenzotriazol esters of Nalpha-butoxycarbonyl-Ndelta-benzyloxycarbonyl-L-ornithine. | [
-0.04770868271589279,
-0.08285800367593765,
-0.02009960636496544,
0.0018676293548196554,
-0.019106371328234673,
0.0063674570992589,
0.05725943669676781,
-0.010137318633496761,
-0.07702870666980743,
-0.02348969876766205,
0.021012574434280396,
-0.034563325345516205,
0.04797251895070076,
0.0007988568395376205,
0.01740213856101036,
-0.020272063091397285,
-0.05329441279172897,
0.004838741850107908,
-0.008127373643219471,
-0.02753402292728424,
-0.005845415871590376,
-0.023334629833698273,
0.030051371082663536,
-0.014060521498322487,
0.06518592685461044,
0.05065425485372543,
0.022966111078858376,
0.045512519776821136,
-0.053115375339984894,
-0.2202257364988327,
0.016704164445400238,
-0.00037449851515702903,
-0.049738138914108276,
-0.03516893461346626,
-0.0680401548743248,
0.05907198414206505,
-0.0051237670704722404,
-0.04040178656578064,
0.012460192665457726,
0.03558743745088577,
0.01565728150308132,
0.004912612959742546,
0.053749002516269684,
-0.028143644332885742,
-0.008155989460647106,
0.022146612405776978,
-0.06881833076477051,
0.030649656429886818,
0.09680519998073578,
0.004730722866952419,
-0.022599821910262108,
0.02303888462483883,
0.07768872380256653,
0.05765045806765556,
-0.018541613593697548,
0.03877175226807594,
-0.023440055549144745,
0.0076045379973948,
-0.036451555788517,
0.02882731892168522,
0.022413091734051704,
-0.005269781686365604,
-0.1303599327802658,
0.036010611802339554,
0.07746303081512451,
0.011105520650744438,
-0.0005839656223542988,
0.00007704785093665123,
0.03280360624194145,
0.046344317495822906,
-0.0665862187743187,
-0.04066510498523712,
-0.05092528089880943,
-0.0009369096951559186,
0.05540318042039871,
0.016443291679024696,
0.010883673094213009,
-0.003819249337539077,
0.039163410663604736,
-0.04811085760593414,
0.039229270070791245,
0.0035066704731434584,
0.002316135913133621,
-0.012412896379828453,
0.028677014634013176,
-0.007620943244546652,
-0.004124283790588379,
-0.02069099247455597,
0.02473272569477558,
-0.031604744493961334,
-0.0299226064234972,
-0.06679385900497437,
0.041559699922800064,
-0.0007000417681410909,
-0.03582978993654251,
-0.04417891427874565,
-0.008875367231667042,
-0.010066753253340721,
0.01053972914814949,
0.3616648316383362,
-0.033625271171331406,
0.04603879898786545,
-0.04135838896036148,
0.043471887707710266,
0.03153898939490318,
-0.036537788808345795,
0.009170353412628174,
-0.016716638579964638,
0.01669536717236042,
0.06164619326591492,
0.07915076613426208,
0.03552353009581566,
0.03694518283009529,
-0.03255118057131767,
-0.048900242894887924,
0.037264734506607056,
0.021107632666826248,
-0.011078117415308952,
-0.1072634607553482,
0.04378809407353401,
-0.03597674146294594,
-0.07488905638456345,
0.00269014248624444,
-0.026065338402986526,
0.002381688915193081,
0.006107384338974953,
-0.013225817121565342,
0.03352605551481247,
0.043182142078876495,
0.00594651373103261,
0.041826050728559494,
0.03421582281589508,
-0.03911605477333069,
-0.014384252019226551,
0.0040175821632146835,
0.015778321772813797,
0.013723121955990791,
-0.04195177182555199,
0.0034674026537686586,
-0.0022926037199795246,
-0.009993384592235088,
-0.07378505915403366,
-0.0871480405330658,
-0.10002629458904266,
-0.1039297953248024,
0.10728540271520615,
0.013070918619632721,
0.00422666035592556,
0.0030663195066154003,
0.014039442874491215,
0.032802071422338486,
0.03157109394669533,
0.03379402682185173,
-0.03060903213918209,
-0.040197763592004776,
0.02053726464509964,
0.03388151526451111,
-0.009336563758552074,
-0.026781542226672173,
0.014935596846044064,
-0.0867084488272667,
0.0054290639236569405,
-0.04656950384378433,
0.11767087876796722,
-0.015546780079603195,
-0.023798374459147453,
-0.009933868423104286,
0.002912019845098257,
-0.005315868649631739,
0.020641997456550598,
0.003654244588688016,
0.03493060916662216,
-0.03533034399151802,
-0.00057157629635185,
-0.08020613342523575,
0.012052055448293686,
-0.021826904267072678,
-0.025906046852469444,
0.028825119137763977,
0.002991731744259596,
0.047777097672224045,
-0.01080318633466959,
-0.053249090909957886,
-0.0056821200996637344,
0.06688462942838669,
0.006778784561902285,
-0.07292765378952026,
-0.024663612246513367,
-0.047087762504816055,
0.05329391360282898,
-0.005598132964223623,
0.03197186067700386,
-0.03196132183074951,
0.0335448682308197,
-0.025512317195534706,
-0.04972093552350998,
-0.08889735490083694,
0.08467980474233627,
-0.012135651893913746,
-0.01267103012651205,
0.12277569621801376,
0.04991144314408302,
0.006449433509260416,
-0.013229588977992535,
-0.022236187011003494,
-0.052080754190683365,
0.01238004770129919,
-0.006930784787982702,
-0.018604518845677376,
-0.014930849894881248,
-0.05867764726281166,
0.0013305946486070752,
-0.03594259172677994,
0.043805964291095734,
-0.028133133426308632,
0.07164033502340317,
0.007939726114273071,
-0.014022910967469215,
0.0688796415925026,
-0.0010385527275502682,
0.016467316076159477,
-0.03181711956858635,
-0.055123843252658844,
-0.29866424202919006,
-0.0168702844530344,
0.05920509994029999,
0.021123012527823448,
0.0832580178976059,
-0.08573884516954422,
-0.00012437516124919057,
-0.02750251069664955,
0.006163623183965683,
0.002993382280692458,
0.10584323108196259,
0.049305662512779236,
-0.014337231405079365,
-0.043094947934150696,
-0.013273443095386028,
-0.014656712301075459,
0.03319514915347099,
0.025042306631803513,
0.01236534584313631,
-0.009730152785778046,
0.02831004187464714,
0.043768323957920074,
0.0032885477412492037,
0.044043924659490585,
0.02977956086397171,
0.009639250114560127,
0.13288728892803192,
-0.015502934344112873,
0.04363907128572464,
-0.03126458823680878,
0.026787763461470604,
0.05413582921028137,
-0.025253761559724808,
-0.0316411592066288,
-0.024353107437491417,
-0.012210904620587826,
0.05178390443325043,
-0.07374269515275955,
0.07254233956336975,
-0.04614536836743355,
-0.03377184271812439,
0.022406917065382004,
-0.0011138200061395764,
-0.06936813145875931,
-0.04249656945466995,
-0.022706782445311546,
0.008871018886566162,
-0.021411549299955368,
0.033242836594581604,
0.034011561423540115,
-0.024277495220303535,
0.030244309455156326,
0.07187170535326004,
-0.03534466773271561,
0.013464611954987049,
0.005809931084513664,
-0.02492551878094673,
-0.03221326693892479,
-0.036683738231658936,
0.027850953862071037,
-0.01861504465341568,
0.07582837343215942,
-0.027183232828974724,
-0.007886157371103764,
-0.04723919555544853,
-0.03856110945343971,
0.001955601619556546,
-0.006737779825925827,
0.08850115537643433,
0.04188442975282669,
0.0555596761405468,
0.0991404727101326,
-0.03826574608683586,
0.08166070282459259,
0.007542967796325684,
0.022390542551875114,
-0.033028118312358856,
0.0039647347293794155,
-0.07039830088615417,
0.026416918262839317,
-0.023896312341094017,
-0.03775186464190483,
0.04213295504450798,
-0.04945523291826248,
0.013313115574419498,
0.039994437247514725,
-0.041677325963974,
0.014466440305113792,
0.04242529347538948,
0.005326700396835804,
-0.0014949953183531761,
-0.030214641243219376,
-0.0030496411491185427,
-0.05931157246232033,
0.01637819968163967,
0.056042008101940155,
-0.23938985168933868,
-0.0007565357955172658,
0.019271230325102806,
0.06012222543358803,
-0.05099741369485855,
0.0266465712338686,
0.04605332389473915,
-0.05913090705871582,
-0.01742568612098694,
-0.0007608062587678432,
0.018413327634334564,
0.008306165225803852,
0.10038508474826813,
0.02874295599758625,
0.009153356775641441,
-0.0132417231798172,
0.0845983698964119,
-0.01660449430346489,
-0.003607194870710373,
-0.014183885417878628,
-0.04394403100013733,
-0.017596952617168427,
0.1655607670545578,
0.0032883400563150644,
0.04195243492722511,
0.05346406251192093,
-0.008532376028597355,
0.03760737180709839,
-0.027898451313376427,
0.029093030840158463,
-0.0020383684895932674,
0.04154883697628975,
0.05614667013287544,
-0.05182396620512009,
0.03763844817876816,
0.027788294479250908,
-0.021848399192094803,
-0.01890702173113823,
-0.04633302241563797,
0.006144167855381966,
-0.039052609354257584,
0.013814382255077362,
-0.008915441110730171,
0.022648315876722336,
0.04120323061943054,
-0.054658468812704086,
-0.029606593772768974,
-0.057507678866386414,
0.029144126921892166,
0.03270535543560982,
0.016683941707015038,
0.03208324313163757,
-0.02502555027604103,
-0.03094164840877056,
0.057597894221544266,
-0.03463694453239441,
-0.0512218251824379,
-0.0016270143678411841,
-0.039158981293439865,
-0.006297187879681587,
-0.0012966871727257967,
-0.029964543879032135,
-0.006721478886902332,
0.045639194548130035,
-0.00903597567230463
] |
PMID:8312 | Yeast hexokinase: substrate-induced association--dissociation reactions in the binding of glucose to hexokinase P-II. | A method is described for the purification of native hexokinases P-I and P-II from yeast using preparative isoelectric focussing to separate the isozymes. The binding of glucose to hexokinase P-II, and the effect of this on the monomer--dimer association--dissociation reaction have been investigated quantitatively by a combination of titrations of intrinsic protein fluorescence and equilibrium ultracentrifugation. Association constants for the monomer-dimer reaction decreased with increasing pH, ionic strength and concentration of glucose. Saturating concentrations of glucose did not bring about complete dissociation of the enzyme showing that both sites were occupired in the dimer. At pH 8.0 and high ionic strength, where the enzyme existed as monomer, the dissociation constant of the enzyme-glucose complex was 3 X 10(-4) mol 1(-1) and was independent of the concentration of enzyme. Binding to the dimeric form at low pH and ionic strength (I=0.02 mol 1(-1), pH less than 7.5) was also independent of enzyme concentration (in the range 10-1000 mug ml-1) but was much weaker. The process could be described by a single dissociation constant, showing that the two available sites on the dimer were equivalent and non-cooperative; values of the intrinsic dissociation constant varied from 2.5 X 10(-3) mol 1(-1) at pH 7.0 to 6 X 10(-3) at pH 6.5. Under intermediate conditions (pH 7.0, ionic strength=0.15 mol 1(-1)), where monomer and dimer coexisted, the binding of glucose showed weak positive cooperatively (Hill coefficient 1.2); in addition, the binding was dependent upon the concentration of enzyme in the direction of stronger binding at lower concentrations. The results show that the phenomenon of half-sites reactivity observed in the binding of glucose to crystalline hexokinase P-II does not occur in solution; the simplest explanation of our finding the two sites to be equivalent is that the dimer results from the homologous association of two identical subunits. | Yeast hexokinase: substrate-induced association--dissociation reactions in the binding of glucose to hexokinase P-II. A method is described for the purification of native hexokinases P-I and P-II from yeast using preparative isoelectric focussing to separate the isozymes. The binding of glucose to hexokinase P-II, and the effect of this on the monomer--dimer association--dissociation reaction have been investigated quantitatively by a combination of titrations of intrinsic protein fluorescence and equilibrium ultracentrifugation. Association constants for the monomer-dimer reaction decreased with increasing pH, ionic strength and concentration of glucose. Saturating concentrations of glucose did not bring about complete dissociation of the enzyme showing that both sites were occupired in the dimer. At pH 8.0 and high ionic strength, where the enzyme existed as monomer, the dissociation constant of the enzyme-glucose complex was 3 X 10(-4) mol 1(-1) and was independent of the concentration of enzyme. Binding to the dimeric form at low pH and ionic strength (I=0.02 mol 1(-1), pH less than 7.5) was also independent of enzyme concentration (in the range 10-1000 mug ml-1) but was much weaker. The process could be described by a single dissociation constant, showing that the two available sites on the dimer were equivalent and non-cooperative; values of the intrinsic dissociation constant varied from 2.5 X 10(-3) mol 1(-1) at pH 7.0 to 6 X 10(-3) at pH 6.5. Under intermediate conditions (pH 7.0, ionic strength=0.15 mol 1(-1)), where monomer and dimer coexisted, the binding of glucose showed weak positive cooperatively (Hill coefficient 1.2); in addition, the binding was dependent upon the concentration of enzyme in the direction of stronger binding at lower concentrations. The results show that the phenomenon of half-sites reactivity observed in the binding of glucose to crystalline hexokinase P-II does not occur in solution; the simplest explanation of our finding the two sites to be equivalent is that the dimer results from the homologous association of two identical subunits. | [
-0.09856484830379486,
0.032985884696245193,
-0.006096709985285997,
0.008323244750499725,
0.014692408964037895,
0.00875049363821745,
0.05132600665092468,
0.03819817677140236,
0.00005864137347089127,
-0.015542279928922653,
-0.022617239505052567,
-0.07732056081295013,
-0.018720509484410286,
0.0871877521276474,
-0.01999923773109913,
-0.01949336938560009,
-0.04709119722247124,
0.030350904911756516,
-0.08856124430894852,
-0.050746649503707886,
0.0008120183483697474,
-0.05619475990533829,
0.05368310213088989,
-0.016850043088197708,
0.045688360929489136,
0.03561856597661972,
-0.021147755905985832,
0.021645020693540573,
-0.04292544722557068,
-0.23903782665729523,
0.013317246921360493,
0.007003979291766882,
0.07246393710374832,
-0.025044940412044525,
-0.01776384934782982,
0.023463882505893707,
-0.010613247752189636,
-0.0188039131462574,
-0.027401404455304146,
0.01638256572186947,
0.06554508209228516,
0.06491640210151672,
-0.01683124527335167,
0.0021761299576610327,
-0.013539472594857216,
-0.048307377845048904,
-0.06344101577997208,
0.007582435850054026,
0.04342259094119072,
0.044633761048316956,
0.0015079394215717912,
0.0034522414207458496,
0.008096062578260899,
0.018935738131403923,
-0.008830389939248562,
0.02095216140151024,
0.023168427869677544,
0.037622347474098206,
0.0009503707406111062,
-0.016719747334718704,
0.02130897529423237,
0.010994117707014084,
-0.12552687525749207,
0.0798761397600174,
0.06731178611516953,
-0.005303489044308662,
-0.022785652428865433,
-0.027632176876068115,
0.044522300362586975,
0.05051909759640694,
-0.02039172314107418,
0.07283677905797958,
0.024856731295585632,
-0.013092531822621822,
0.019884435459971428,
0.010587983764708042,
0.047093745321035385,
0.009458109736442566,
0.061242155730724335,
0.0293295718729496,
-0.04798642173409462,
-0.012513178400695324,
-0.00007220921543193981,
-0.04194026440382004,
-0.04987790808081627,
0.040520377457141876,
0.06313484162092209,
-0.0254229586571455,
0.031046349555253983,
0.01778816431760788,
0.009689797647297382,
-0.07063084840774536,
-0.002404101425781846,
0.001712776138447225,
-0.11444535106420517,
0.1014409288764,
0.09943398088216782,
-0.020084325224161148,
0.05750139802694321,
0.40305468440055847,
-0.04149259626865387,
0.01631891541182995,
-0.040387868881225586,
-0.006380891893059015,
-0.015634281560778618,
-0.047456178814172745,
-0.020854005590081215,
-0.0008706938242539763,
-0.021205492317676544,
0.016084041446447372,
0.05551059544086456,
0.007165512070059776,
-0.049778737127780914,
-0.0721823051571846,
-0.03898123651742935,
-0.008014732971787453,
0.024219609797000885,
0.04655737429857254,
-0.0350889153778553,
-0.009190129116177559,
-0.029125379398465157,
0.00529618700966239,
-0.00016420248721260577,
0.03724776953458786,
0.026129448786377907,
0.04904285818338394,
-0.021450357511639595,
0.07466565817594528,
0.058997053653001785,
-0.029731234535574913,
0.05501740425825119,
0.04715414717793465,
-0.018733976408839226,
-0.012145157903432846,
-0.07542230933904648,
-0.046611569821834564,
0.04891262203454971,
-0.03408776596188545,
0.00426111463457346,
0.011176898144185543,
0.01573808118700981,
-0.02476385608315468,
-0.03295362740755081,
-0.04361360892653465,
-0.025793181732296944,
0.04250087961554527,
-0.0024985356722027063,
-0.00232483702711761,
0.004447554238140583,
-0.0009053923422470689,
0.008788264356553555,
0.012748961336910725,
-0.0340593047440052,
0.025784963741898537,
-0.012111346237361431,
0.018755529075860977,
0.01919969730079174,
-0.08494012802839279,
0.03698226064443588,
0.010391718707978725,
-0.08255086094141006,
-0.005958784371614456,
0.02271314710378647,
0.06768332421779633,
0.0354636125266552,
-0.010967315174639225,
0.006588644813746214,
0.016040148213505745,
-0.00489511014893651,
-0.004723662976175547,
0.019561702385544777,
-0.042779311537742615,
0.009542572312057018,
-0.021938659250736237,
-0.04264961928129196,
0.0012713755713775754,
0.0116364024579525,
0.050418563187122345,
-0.024915169924497604,
0.017785126343369484,
0.04417763277888298,
-0.02268247678875923,
0.03948419913649559,
0.00591198680922389,
0.01733042672276497,
-0.013891298323869705,
-0.019298983737826347,
0.00821472704410553,
-0.02784697897732258,
0.033809565007686615,
-0.08051960170269012,
0.038170717656612396,
-0.11678333580493927,
0.04530051350593567,
-0.027012797072529793,
0.040636755526065826,
0.0004242057038936764,
0.037762630730867386,
-0.01756826415657997,
-0.0351056307554245,
0.14047788083553314,
0.018743282184004784,
-0.04114910587668419,
0.04622885212302208,
0.021061066538095474,
0.019014814868569374,
0.007376690860837698,
0.04573329910635948,
0.018182383850216866,
0.0067370072938501835,
-0.03830516338348389,
-0.05195237323641777,
-0.08255914598703384,
-0.035450950264930725,
-0.05631708353757858,
0.04711782559752464,
0.027182070538401604,
0.03243015334010124,
0.007191028445959091,
0.03376518189907074,
0.01775268279016018,
-0.04488694295287132,
-0.06065152585506439,
-0.29682523012161255,
0.03752729669213295,
-0.032682232558727264,
0.006380041595548391,
0.015295167453587055,
-0.07952568680047989,
-0.0016225433209910989,
-0.02737393230199814,
-0.005268142092972994,
-0.002444251673296094,
0.025966543704271317,
0.01693551056087017,
-0.01696518249809742,
-0.04528871923685074,
-0.018503520637750626,
0.03238126263022423,
0.026620998978614807,
-0.014056066982448101,
-0.02177772857248783,
0.008451303467154503,
-0.017900343984365463,
-0.022247912362217903,
0.049664877355098724,
-0.033227887004613876,
0.05916229262948036,
-0.019116021692752838,
0.08204153925180435,
0.0010248039616271853,
0.038276754319667816,
-0.0315336212515831,
0.022057686001062393,
0.06645289808511734,
-0.040998607873916626,
-0.07265941798686981,
-0.04517560824751854,
-0.023141151294112206,
-0.02141449972987175,
-0.06630399823188782,
0.05229468643665314,
-0.021577658131718636,
-0.03211453929543495,
-0.0012533898698166013,
-0.03192589432001114,
-0.048221688717603683,
0.009684753604233265,
-0.021362880244851112,
0.03687570244073868,
-0.025943219661712646,
0.019232407212257385,
-0.016156986355781555,
0.08378369361162186,
-0.01919702999293804,
0.020290346816182137,
-0.026523033156991005,
0.03292100504040718,
-0.011903728358447552,
-0.030985424295067787,
0.041914843022823334,
-0.04710537940263748,
0.05233103781938553,
-0.04141976684331894,
-0.04531857371330261,
-0.04332328960299492,
0.03647893667221069,
0.05221547186374664,
-0.0003665231342893094,
0.020785316824913025,
-0.051634274423122406,
0.040171172469854355,
0.02448882907629013,
0.006834209896624088,
0.0683460608124733,
0.0022361467126756907,
0.011858421377837658,
0.018611010164022446,
0.0438833050429821,
-0.015540453605353832,
-0.024281729012727737,
-0.04631480574607849,
0.02850196696817875,
0.004949962720274925,
0.012791863642632961,
0.06265327334403992,
-0.00857020914554596,
0.000847873103339225,
0.04877743124961853,
0.03448670357465744,
0.0285173486918211,
0.009535534307360649,
-0.02372099459171295,
-0.001932648359797895,
0.00887587945908308,
-0.0019182146061211824,
-0.04352518543601036,
0.00453172205016017,
0.056207988411188126,
-0.2666453421115875,
0.03952993080019951,
-0.04227233678102493,
0.07947999984025955,
-0.030862903222441673,
0.03453294560313225,
0.06387194246053696,
-0.04226623848080635,
-0.01645849645137787,
0.007404698058962822,
-0.0018649904523044825,
0.0016459160251542926,
0.033190708607435226,
0.0485716238617897,
-0.03799054026603699,
0.057161781936883926,
0.023040786385536194,
-0.06491971760988235,
-0.00958979595452547,
-0.02058233693242073,
-0.01453456748276949,
-0.00664860662072897,
0.15937429666519165,
-0.03580056503415108,
-0.0001382638729410246,
0.010902446694672108,
0.011337850242853165,
0.04273055121302605,
0.01619931496679783,
0.02750495634973049,
-0.047105927020311356,
-0.003008953295648098,
0.10723648965358734,
-0.04345862194895744,
0.041279904544353485,
0.05762042477726936,
0.014597047120332718,
-0.06362096965312958,
0.015707749873399734,
0.004251075908541679,
0.010591135360300541,
-0.03651179373264313,
-0.06813731044530869,
-0.017711447551846504,
0.03880767896771431,
-0.049582257866859436,
-0.020922215655446053,
-0.007146503776311874,
-0.03378988802433014,
0.00318719819188118,
0.0030478553380817175,
-0.04372544586658478,
-0.0006975668948143721,
0.007284827996045351,
-0.005650543607771397,
-0.021263478323817253,
-0.06016813963651657,
-0.07698039710521698,
-0.05026130750775337,
0.001630274928174913,
0.037834253162145615,
0.06404242664575577,
-0.04187730327248573,
0.041080668568611145,
-0.017293425276875496
] |
PMID:8313 | Affinity chromatography and binding studies on immobilized 5'-monophosphate and adenosine 2',5'-bisphosphate of nicotinamide nucleotide transhydrogenase from Pseudomonas aeruginosa. | 1. Nicotinamide nucleotide transhydrogenase from Pseudomonas aeruginosa was purified to apparent homogeneity with an improved method employing affinity chromatography on N6-(6aminohexyl)-adenosine 2', 5'-bisphosphate-Sepharose 4B. 2. Polyacrylamide gel electrophoresis of the purified transhydrogenase carried out in the presence of sodium dodecyl sulphate, indicated a minimal molecular weight of 55000 +/- 2000. 3. The kinetic and regulatory properties of the purified transhydrogenase resembled those of the crude enzyme, i.e., NADPH, adenosine 2'-monophosphate and Ca2+ were activators whereas NADP+ was inhibitory. 4. Nicotinamide nucleotide-specific release of binding of the transhydrogenase to N6-(6-aminohexyl)-adenosine-2',5'-bisphosphate-Sepharose and N6-(-aminohexyl)-adenosine-5'-monophosphate-Sepharose suggests the presence of at least two separate binding sites for nicotinamide nucleotides, one that is specific for NADP(H) and one that binds both NAD(H) and NADP(H). 5. Binding of transhydrogenase to N6-)6-aminohexyl)-adenosine-2',5'-bisphosphate-Sepharose and activation of the enzyme by adenosine-2',5'-bisphophate showed a marked pH dependence. In contrast, inhibition of the Ca2+-activated enzyme by adenosine 2',5'-bisphosphate was virtually constant at various pH values. This descrepancy was interpreted to indicate the existence of separate nucleotide-binding effector and active sites. | Affinity chromatography and binding studies on immobilized 5'-monophosphate and adenosine 2',5'-bisphosphate of nicotinamide nucleotide transhydrogenase from Pseudomonas aeruginosa. 1. Nicotinamide nucleotide transhydrogenase from Pseudomonas aeruginosa was purified to apparent homogeneity with an improved method employing affinity chromatography on N6-(6aminohexyl)-adenosine 2', 5'-bisphosphate-Sepharose 4B. 2. Polyacrylamide gel electrophoresis of the purified transhydrogenase carried out in the presence of sodium dodecyl sulphate, indicated a minimal molecular weight of 55000 +/- 2000. 3. The kinetic and regulatory properties of the purified transhydrogenase resembled those of the crude enzyme, i.e., NADPH, adenosine 2'-monophosphate and Ca2+ were activators whereas NADP+ was inhibitory. 4. Nicotinamide nucleotide-specific release of binding of the transhydrogenase to N6-(6-aminohexyl)-adenosine-2',5'-bisphosphate-Sepharose and N6-(-aminohexyl)-adenosine-5'-monophosphate-Sepharose suggests the presence of at least two separate binding sites for nicotinamide nucleotides, one that is specific for NADP(H) and one that binds both NAD(H) and NADP(H). 5. Binding of transhydrogenase to N6-)6-aminohexyl)-adenosine-2',5'-bisphosphate-Sepharose and activation of the enzyme by adenosine-2',5'-bisphophate showed a marked pH dependence. In contrast, inhibition of the Ca2+-activated enzyme by adenosine 2',5'-bisphosphate was virtually constant at various pH values. This descrepancy was interpreted to indicate the existence of separate nucleotide-binding effector and active sites. | [
-0.07454188913106918,
0.010251477360725403,
-0.07062641531229019,
0.005889671389013529,
0.03886245936155319,
-0.01614443212747574,
0.08214357495307922,
0.02095494419336319,
-0.06800474971532822,
-0.0233700443059206,
0.0599677599966526,
-0.07554018497467041,
-0.0064064692705869675,
0.014638249762356281,
-0.029311440885066986,
-0.028974562883377075,
0.0004685776075348258,
-0.0024022662546485662,
-0.0017633788520470262,
-0.034404706209897995,
-0.015537942759692669,
-0.04791295900940895,
0.022122783586382866,
0.01692810282111168,
0.0474991537630558,
0.07379811257123947,
0.02015896700322628,
0.010290979407727718,
-0.0636318176984787,
-0.21129553020000458,
-0.011668884195387363,
-0.016454113647341728,
0.005882119294255972,
-0.09033828973770142,
0.008982768282294273,
0.040900252759456635,
0.005335807800292969,
0.016813239082694054,
0.03713368624448776,
-0.0009045404149219394,
0.013751531019806862,
0.006706153508275747,
0.00007975108019309118,
0.0386335663497448,
-0.0251400638371706,
-0.031805746257305145,
-0.05134333297610283,
-0.008930191397666931,
0.09592443704605103,
-0.03357917070388794,
-0.02348831668496132,
0.017096582800149918,
-0.001224546111188829,
0.007048159372061491,
0.013822057284414768,
0.002246434334665537,
0.1012582778930664,
0.04851103946566582,
-0.03468078747391701,
-0.021209539845585823,
0.021983476355671883,
-0.01116133201867342,
-0.13723494112491608,
0.06390488147735596,
0.1015639528632164,
-0.058162443339824677,
0.03791840746998787,
-0.017569629475474358,
0.07060492038726807,
0.022227216511964798,
-0.07463469356298447,
0.03819910064339638,
-0.021712196990847588,
-0.029700718820095062,
0.022168265655636787,
0.02503512054681778,
-0.009234481491148472,
-0.013693268410861492,
0.05740705877542496,
-0.0033457244280725718,
-0.02592644840478897,
0.016149267554283142,
0.0021593663841485977,
-0.03781825676560402,
-0.05586101487278938,
0.06435375660657883,
0.050574351102113724,
-0.028411664068698883,
0.03509143739938736,
0.03257669135928154,
0.006271913181990385,
-0.05785203352570534,
0.0035663731396198273,
-0.02984427474439144,
-0.05916912108659744,
0.01338051538914442,
-0.02156992256641388,
-0.01581268012523651,
0.054583337157964706,
0.3480028510093689,
-0.01446535438299179,
0.0321166031062603,
-0.046211082488298416,
-0.047594860196113586,
-0.028012804687023163,
-0.062196508049964905,
-0.04045924171805382,
0.025799307972192764,
0.014359939843416214,
0.049930572509765625,
0.034895047545433044,
-0.058797892183065414,
0.01952032372355461,
-0.08062820881605148,
-0.027166735380887985,
0.008992760442197323,
0.019874729216098785,
-0.00021828398166690022,
-0.037891484797000885,
0.04923412203788757,
-0.0242709219455719,
0.0028604178223758936,
0.026541193947196007,
0.017939148470759392,
0.05113910511136055,
-0.0011617322452366352,
-0.04645204916596413,
0.09496201574802399,
0.05880220979452133,
-0.024269554764032364,
0.06019091233611107,
0.010996977798640728,
-0.036947861313819885,
0.0471075065433979,
0.023061459884047508,
-0.008501563221216202,
0.03413374349474907,
-0.06526126712560654,
0.025578755885362625,
-0.04681877791881561,
0.007129156030714512,
-0.056440021842718124,
-0.04502197727560997,
0.0010805753991007805,
-0.041982077062129974,
0.10304335504770279,
-0.03482907637953758,
0.031335510313510895,
0.008293885737657547,
-0.043981555849313736,
0.0032246550545096397,
0.11068674921989441,
-0.01132237259298563,
0.06619662791490555,
-0.015539427287876606,
0.05711188167333603,
0.003581159980967641,
-0.05784265324473381,
0.018731074407696724,
0.0052262828685343266,
-0.10484034568071365,
-0.042932361364364624,
0.03448740392923355,
0.04568135365843773,
0.012778394855558872,
-0.031422436237335205,
-0.024456532672047615,
-0.00517076812684536,
0.00389622594229877,
0.012206015177071095,
-0.03521290048956871,
-0.018105845898389816,
-0.03320857509970665,
0.01158864051103592,
-0.05223970115184784,
0.032589223235845566,
0.0018787261797115207,
0.09153739362955093,
0.036424972116947174,
-0.015977464616298676,
-0.05570492893457413,
-0.003723414381965995,
-0.014754747040569782,
0.014250095933675766,
0.034039970487356186,
0.0557769276201725,
-0.07727260887622833,
0.04180688038468361,
-0.019741781055927277,
0.08434198051691055,
-0.008603544905781746,
0.002187154721468687,
-0.08877547085285187,
0.06210027262568474,
-0.008909620344638824,
-0.033429041504859924,
-0.042302947491407394,
0.051508840173482895,
0.00961353164166212,
-0.004790026694536209,
0.10946839302778244,
0.022409994155168533,
0.0033206085208803415,
0.028004739433526993,
-0.011361704207956791,
0.010500993579626083,
-0.059272363781929016,
0.05252498388290405,
0.0019442002521827817,
0.05561750382184982,
-0.030763981863856316,
0.00546488119289279,
-0.06019261106848717,
0.012759209610521793,
-0.09749892354011536,
-0.004591254983097315,
-0.03350535035133362,
0.02099820040166378,
0.1008421927690506,
0.01640874147415161,
-0.0008749659173190594,
-0.02538977935910225,
-0.019916964694857597,
-0.29606547951698303,
0.01733499951660633,
0.032761216163635254,
-0.027720574289560318,
0.027963912114501,
0.01709163188934326,
0.05279037728905678,
-0.0024426556192338467,
-0.038015659898519516,
-0.047341104596853256,
0.062444958835840225,
0.06281252205371857,
-0.0476062074303627,
-0.04120621830224991,
-0.06380905210971832,
0.006546461954712868,
-0.012020920403301716,
-0.015166444703936577,
0.01825009472668171,
0.024662919342517853,
0.05177691951394081,
0.013694638386368752,
-0.038823869079351425,
0.030769504606723785,
0.04114798083901405,
-0.005896170157939196,
0.07350018620491028,
-0.018748996779322624,
-0.021326029673218727,
0.012820630334317684,
0.02602476254105568,
0.06172152981162071,
-0.058394234627485275,
-0.09704593569040298,
-0.08507341891527176,
-0.024749258533120155,
-0.0725705698132515,
-0.06402435153722763,
0.028835328295826912,
-0.02200082689523697,
0.019383445382118225,
0.021793227642774582,
0.0008989936322905123,
-0.057694196701049805,
-0.028526946902275085,
-0.019934266805648804,
-0.03305705264210701,
0.02090289443731308,
-0.0009626482496969402,
-0.0026872027665376663,
0.055556520819664,
0.018697449937462807,
0.002324554370716214,
-0.06889566779136658,
0.020093534141778946,
-0.003142694244161248,
-0.039660677313804626,
0.009010550566017628,
-0.06688470393419266,
0.010687348432838917,
-0.0485040545463562,
0.009060856886208057,
-0.013609372079372406,
0.0045520467683672905,
-0.008285083808004856,
-0.026011284440755844,
0.03239148110151291,
0.012096086516976357,
0.04409746080636978,
-0.0181056447327137,
0.012132922187447548,
0.06551767140626907,
-0.05812794715166092,
0.03717850521206856,
0.00920153222978115,
0.01351014245301485,
-0.02183554880321026,
-0.06054428219795227,
-0.04238766431808472,
-0.009340068325400352,
0.006583410780876875,
-0.008013923652470112,
0.08215529471635818,
-0.03794582188129425,
-0.044502995908260345,
-0.0017557005630806088,
0.06376677006483078,
-0.06252030283212662,
0.06843230873346329,
0.030653545632958412,
0.0011212449753656983,
-0.02332739718258381,
0.013316698372364044,
-0.012368976138532162,
0.059572845697402954,
0.013630108907818794,
-0.25300562381744385,
-0.024089181795716286,
0.03279639407992363,
0.004513205494731665,
-0.00442076800391078,
0.03663242980837822,
0.04957103729248047,
-0.03571274131536484,
-0.012248155660927296,
0.016889529302716255,
-0.0025554844178259373,
0.022587159648537636,
0.04173491522669792,
0.053053516894578934,
0.002432138193398714,
0.03145932778716087,
0.056628745049238205,
-0.03924676403403282,
0.03340692073106766,
0.004810174461454153,
0.03899552673101425,
-0.05122837796807289,
0.14602038264274597,
-0.008545450866222382,
0.025959951803088188,
0.03416142612695694,
-0.024270545691251755,
0.0032237968407571316,
-0.04443216696381569,
0.030445009469985962,
0.022400962188839912,
0.03839420899748802,
0.08822958171367645,
-0.062050044536590576,
0.0014026392018422484,
0.023425141349434853,
0.0031791802030056715,
0.02000383473932743,
-0.02306850254535675,
0.037021562457084656,
-0.03355902433395386,
0.007113583851605654,
-0.03878467157483101,
-0.01772369258105755,
0.07120585441589355,
-0.05660291761159897,
-0.058220624923706055,
-0.022688236087560654,
0.02199435420334339,
0.07319965958595276,
-0.011810646392405033,
-0.008649981580674648,
0.09165417402982712,
0.03820863738656044,
0.015376077964901924,
-0.02839076891541481,
-0.035311054438352585,
-0.02948073297739029,
-0.025051183998584747,
-0.04164399951696396,
-0.015532152727246284,
0.038414523005485535,
-0.021194731816649437,
0.08360942453145981,
0.03833695873618126
] |
PMID:8314 | Somatic antigens of shigella. Structural investigation on the O-specific polysaccharide chain of Shigella dysenteriae type 1 lipopolysaccharide. | The O-specific polysaccharide obtained from the lipopolysaccharide of Shigella dysenteriae type 1 (Shigella shiga) by mild acid hydrolysis followed by fractionation on Sephadex G-50 was found to be identical to that desribed by Morgan's group and was composed of L-rhamnose, D-galactose and N-acetyl-D-glycosamine in a ratio 2:1:1. On the basis of methylation analysis data the polysaccharide was proved to be a linear chain of monosaccharide residues in pyranose forms substituted at position 3, except for that of galactose substituted at position 2. Selective cleavage, based on the N-deacetylation reaction of the polymer, together with determination of linkage configurations by chromic anhydride oxidation showed that the O-specific polysaccharide is built up of repeating tetrasaccharide units whose proposed structure is given below -3)-alpha-L-Rhap (1-3)-alpha-L-Rhap(1-2)-alpha-D-Galp(1-3)-alphapD-GlcNAcp(1- where RHAP = rhamnopyranose, Galp = galactopyranose, and GlcNAcp = N-acetyl-glucosamine. The present findings confirmed the considerations of Heidelberger on the substitution patterns of L-rhamnose and D-galactose residues from the results of serological studies. | Somatic antigens of shigella. Structural investigation on the O-specific polysaccharide chain of Shigella dysenteriae type 1 lipopolysaccharide. The O-specific polysaccharide obtained from the lipopolysaccharide of Shigella dysenteriae type 1 (Shigella shiga) by mild acid hydrolysis followed by fractionation on Sephadex G-50 was found to be identical to that desribed by Morgan's group and was composed of L-rhamnose, D-galactose and N-acetyl-D-glycosamine in a ratio 2:1:1. On the basis of methylation analysis data the polysaccharide was proved to be a linear chain of monosaccharide residues in pyranose forms substituted at position 3, except for that of galactose substituted at position 2. Selective cleavage, based on the N-deacetylation reaction of the polymer, together with determination of linkage configurations by chromic anhydride oxidation showed that the O-specific polysaccharide is built up of repeating tetrasaccharide units whose proposed structure is given below -3)-alpha-L-Rhap (1-3)-alpha-L-Rhap(1-2)-alpha-D-Galp(1-3)-alphapD-GlcNAcp(1- where RHAP = rhamnopyranose, Galp = galactopyranose, and GlcNAcp = N-acetyl-glucosamine. The present findings confirmed the considerations of Heidelberger on the substitution patterns of L-rhamnose and D-galactose residues from the results of serological studies. | [
-0.07151897251605988,
-0.04500887170433998,
0.005130504257977009,
-0.021322784945368767,
0.03058217279613018,
-0.06318609416484833,
0.006601697765290737,
0.033808618783950806,
-0.030911456793546677,
-0.020670950412750244,
0.042321376502513885,
-0.09028580039739609,
0.0018892685184255242,
-0.02024771459400654,
-0.03117039054632187,
-0.03886757045984268,
-0.0012445427710190415,
0.00523493904620409,
-0.05691421031951904,
0.08021751046180725,
0.07020759582519531,
-0.05910634249448776,
-0.006230450700968504,
0.02032824605703354,
0.08935359120368958,
0.06091383472084999,
0.023695185780525208,
-0.013839312829077244,
-0.025101639330387115,
-0.18867002427577972,
-0.02218872867524624,
-0.03644939139485359,
0.01763433963060379,
-0.009147371165454388,
-0.039430126547813416,
-0.016642412170767784,
-0.019364457577466965,
-0.008661541156470776,
0.0021707694977521896,
-0.011367952451109886,
0.051407985389232635,
-0.031078549101948738,
0.010107950307428837,
0.029484126716852188,
-0.01015311200171709,
-0.0014242702163755894,
-0.058334413915872574,
0.053773850202560425,
0.05528899282217026,
0.016262231394648552,
-0.007336220238357782,
0.032163865864276886,
0.007535153534263372,
0.04494066163897514,
0.026088038459420204,
0.002838625106960535,
-0.0062723662704229355,
0.03441183269023895,
0.01500837691128254,
-0.0070816148072481155,
-0.04242097958922386,
0.04779313504695892,
-0.09386704862117767,
0.09329799562692642,
0.01532551646232605,
0.005053021479398012,
0.028281789273023605,
0.006912462413311005,
0.05733886733651161,
0.05725807324051857,
-0.07562322914600372,
-0.0008841723320074379,
-0.025022894144058228,
0.021418074145913124,
0.014277065172791481,
0.021977007389068604,
0.006449579261243343,
-0.011922565288841724,
0.0464044064283371,
0.038267794996500015,
0.001995830098167062,
0.0338149331510067,
0.026715220883488655,
-0.08994535356760025,
-0.042908862233161926,
-0.023956971243023872,
0.040226247161626816,
-0.025866443291306496,
0.04727858677506447,
0.015033623203635216,
0.040334515273571014,
-0.04044357314705849,
-0.03388310223817825,
0.04031364992260933,
-0.07322245836257935,
0.034391727298498154,
0.015695955604314804,
-0.04339153319597244,
0.07408647239208221,
0.3589058220386505,
-0.09959227591753006,
-0.012196166440844536,
0.01187965553253889,
0.021485278382897377,
0.04397651180624962,
-0.012577799148857594,
0.006475213449448347,
-0.017991475760936737,
0.029940886422991753,
0.047241806983947754,
0.054348770529031754,
0.02552735060453415,
0.000259611988440156,
-0.09710859507322311,
-0.017874352633953094,
-0.008055292069911957,
-0.01433844119310379,
0.03722250461578369,
-0.011486741714179516,
0.06489554047584534,
-0.05837083235383034,
-0.01165519654750824,
0.044825293123722076,
0.0031937211751937866,
0.04207892715930939,
0.042978037148714066,
-0.009170844219624996,
0.0874696895480156,
0.018961232155561447,
0.019096925854682922,
0.048598695546388626,
0.03995063528418541,
0.0028501171618700027,
-0.022431254386901855,
-0.020615018904209137,
0.05732084810733795,
0.06182233989238739,
-0.010836669243872166,
-0.013200943358242512,
-0.021484771743416786,
-0.07565999031066895,
-0.057946037501096725,
-0.007103057578206062,
-0.06864627450704575,
-0.08180665224790573,
0.05440840125083923,
-0.048480093479156494,
0.017434757202863693,
-0.047495365142822266,
0.025766558945178986,
-0.009861372411251068,
0.07912395149469376,
-0.03522544354200363,
0.049169525504112244,
-0.03247065842151642,
0.03326388821005821,
-0.03113221563398838,
-0.022044502198696136,
-0.012646893970668316,
-0.021538184955716133,
-0.010244416072964668,
0.05638309195637703,
0.011575935408473015,
0.12261324375867844,
-0.00434148171916604,
-0.10321595519781113,
0.0010412606643512845,
0.0011493503116071224,
0.025594273582100868,
-0.03550582006573677,
0.02203739620745182,
0.031243907287716866,
-0.003877554787322879,
-0.011729964055120945,
-0.03590841963887215,
0.014466524124145508,
-0.019941851496696472,
0.045687802135944366,
0.041426170617341995,
0.05352911353111267,
0.03840526565909386,
-0.024514660239219666,
-0.0596267506480217,
-0.05654188618063927,
0.03642002120614052,
-0.007858045399188995,
-0.020177407190203667,
0.03463508188724518,
0.024807974696159363,
-0.002272003795951605,
-0.004645754583179951,
-0.0193845983594656,
-0.09359704703092575,
0.05036158114671707,
0.004872721154242754,
0.003684657160192728,
-0.039751600474119186,
0.012298074550926685,
0.02253318764269352,
-0.024944279342889786,
0.10512880235910416,
0.05727207660675049,
-0.0031377202831208706,
0.007608187850564718,
0.03224862366914749,
-0.02724856697022915,
0.10147705674171448,
0.013800634071230888,
-0.03678259998559952,
-0.03184204176068306,
-0.03497488051652908,
0.025042418390512466,
-0.12327714264392853,
0.025475336238741875,
-0.029085658490657806,
0.02286630868911743,
0.010802976787090302,
0.0010380517924204469,
0.05033073574304581,
0.029100824147462845,
0.005864120088517666,
-0.019062738865613937,
-0.028014441952109337,
-0.2794056236743927,
0.014935881830751896,
-0.05055716261267662,
-0.05075705796480179,
-0.0017379240598529577,
-0.050788264721632004,
-0.04816543310880661,
-0.03587966784834862,
0.05424670875072479,
-0.022829445078969002,
0.08511731028556824,
0.05346974730491638,
-0.033949919044971466,
-0.06839529424905777,
-0.0296957865357399,
0.037132628262043,
0.060168586671352386,
-0.045965660363435745,
0.04472317919135094,
0.051781006157398224,
0.022948548197746277,
-0.025587458163499832,
0.025449560955166817,
0.07045089453458786,
0.018595002591609955,
-0.0793011337518692,
0.14390110969543457,
0.04876895248889923,
0.05378223583102226,
-0.010979689657688141,
0.021714061498641968,
0.025336774066090584,
0.010335457511246204,
-0.04348612576723099,
0.026911983266472816,
0.067878358066082,
-0.05017439275979996,
-0.06998094916343689,
0.054138872772455215,
0.009888452477753162,
0.03295384719967842,
-0.02425757795572281,
-0.040758900344371796,
-0.07539216428995132,
0.0022279152180999517,
-0.07803554087877274,
-0.026150666177272797,
-0.006496355403214693,
0.033492498099803925,
0.04340836778283119,
-0.049451179802417755,
-0.0312846377491951,
0.03277702257037163,
0.05149395391345024,
-0.04003563150763512,
0.0002836027997545898,
-0.013367238454520702,
-0.011013833805918694,
-0.04462436959147453,
-0.008360045030713081,
-0.029941052198410034,
-0.008484735153615475,
-0.017895955592393875,
0.02704590931534767,
-0.04838287830352783,
-0.002003295812755823,
0.0046263462863862514,
-0.0353463813662529,
0.020209211856126785,
-0.008761636912822723,
-0.01281102653592825,
0.06656121462583542,
-0.03588617220520973,
0.015520329587161541,
0.022844752296805382,
0.08631826937198639,
-0.028274407610297203,
-0.06716084480285645,
-0.0774981901049614,
0.012998983263969421,
0.02732500433921814,
-0.008475396782159805,
0.06654531508684158,
0.05313779041171074,
-0.0032306965440511703,
0.028765056282281876,
-0.017020147293806076,
-0.015310991555452347,
0.00638822466135025,
0.06399857252836227,
-0.01686139777302742,
0.01841006800532341,
0.005600554868578911,
-0.04629293084144592,
-0.0022087590768933296,
0.005033574532717466,
-0.23371142148971558,
0.018900573253631592,
0.002370130503550172,
0.043869439512491226,
-0.0238200556486845,
0.038918402045965195,
0.02972104772925377,
-0.07377148419618607,
0.014452471397817135,
0.018373921513557434,
-0.0013320798752829432,
0.0775475725531578,
0.08355256915092468,
-0.054354894906282425,
-0.013404874131083488,
-0.013628083281219006,
0.028344519436359406,
0.04926278442144394,
0.0710224062204361,
-0.020525995641946793,
-0.02006085403263569,
-0.031207960098981857,
0.13157741725444794,
0.024176830425858498,
-0.051214735954999924,
-0.005188059061765671,
-0.036316804587841034,
0.018084799870848656,
-0.034939173609018326,
-0.002587905153632164,
0.02418777532875538,
0.02905016951262951,
0.09027600288391113,
-0.05556819215416908,
0.017621006816625595,
-0.010031037032604218,
-0.02871287800371647,
-0.03693319857120514,
-0.03882177546620369,
0.045821137726306915,
-0.02231181412935257,
-0.028540100902318954,
-0.08828548341989517,
-0.058237530291080475,
0.08737002313137054,
-0.02184981480240822,
-0.03587151691317558,
-0.04801497235894203,
-0.022596875205636024,
-0.004943293519318104,
-0.016340801492333412,
-0.029407961294054985,
-0.05091046541929245,
-0.0028705804143100977,
0.002505713142454624,
-0.0012734591728076339,
-0.048288457095623016,
0.055739786475896835,
-0.025698736310005188,
-0.029475249350070953,
-0.013412063010036945,
-0.04245012253522873,
-0.07703211903572083,
0.08015218377113342,
0.03085208497941494
] |
PMID:8315 | Purification and properties of 3-hexulosephosphate synthase from Methylomonas M 15. | 3-Hexulosephosphate synthase, the first enzyme of the ribulose monophosphate cycle, was purified 15-fold from methanol-grown Methylomonas M 15. The purification procedure involved chromatography on DEAE-cellulose, Sephadex G-75, and DEAE-Sephadex A-50. The purified enzyme was more than 95% pure as judged by analytical polyacrylamide gel electrophoresis. The molecular weight was calculated to be 43000 from sedimentation equilibrium experiments. Electrophoresis in sodium dodecylsulfate gels gave a single band corresponding to a molecular weight of 22000. The enzyme catalyzes specifically the condensation formaldehyde with ribulose 5-phosphate to yield D-arabino-3-hexulose 6-phosphate. The Km values were found to be 1.1 mM for formaldehyde and 1.6 mM for ribulose 5-phosphate. A bivalent cation is essential for activity and stability of the enzyme, Mg2+ and Mn2+ serve best for this purpose. The optimum of pH for enzyme activity is 7.5--8.0. | Purification and properties of 3-hexulosephosphate synthase from Methylomonas M 15. 3-Hexulosephosphate synthase, the first enzyme of the ribulose monophosphate cycle, was purified 15-fold from methanol-grown Methylomonas M 15. The purification procedure involved chromatography on DEAE-cellulose, Sephadex G-75, and DEAE-Sephadex A-50. The purified enzyme was more than 95% pure as judged by analytical polyacrylamide gel electrophoresis. The molecular weight was calculated to be 43000 from sedimentation equilibrium experiments. Electrophoresis in sodium dodecylsulfate gels gave a single band corresponding to a molecular weight of 22000. The enzyme catalyzes specifically the condensation formaldehyde with ribulose 5-phosphate to yield D-arabino-3-hexulose 6-phosphate. The Km values were found to be 1.1 mM for formaldehyde and 1.6 mM for ribulose 5-phosphate. A bivalent cation is essential for activity and stability of the enzyme, Mg2+ and Mn2+ serve best for this purpose. The optimum of pH for enzyme activity is 7.5--8.0. | [
-0.09933193773031235,
-0.021489296108484268,
-0.003955036401748657,
-0.05107253044843674,
0.02104969695210457,
-0.025034034624695778,
-0.009876148775219917,
0.018442630767822266,
-0.0592384897172451,
0.03445097804069519,
0.0005680668982677162,
-0.08158091455698013,
0.003926953766494989,
0.0020822836086153984,
0.004908974282443523,
-0.01560250949114561,
-0.03351167216897011,
0.016391579061746597,
-0.0459628663957119,
0.010503283701837063,
0.06729310750961304,
-0.00287729618139565,
-0.031420547515153885,
-0.015166283585131168,
0.08041930198669434,
0.0022878115996718407,
0.059854790568351746,
0.03438287228345871,
-0.010947464033961296,
-0.2044660598039627,
-0.002370761474594474,
-0.026217861101031303,
0.004065524786710739,
-0.07876633107662201,
0.033562835305929184,
0.004197843372821808,
-0.036522384732961655,
-0.04052245244383812,
-0.013724228367209435,
0.07526227831840515,
-0.018708400428295135,
-0.011540780775249004,
0.03982182964682579,
0.06784137338399887,
-0.06364678591489792,
-0.032487720251083374,
-0.025880979374051094,
-0.0019904745277017355,
0.08422557264566422,
0.016049034893512726,
-0.0028534771408885717,
-0.032880160957574844,
0.016176221892237663,
-0.07108660042285919,
0.009049692191183567,
0.0016192285111173987,
0.05456720292568207,
0.03186384588479996,
-0.03422117233276367,
0.02088243141770363,
0.02388988807797432,
0.03276098147034645,
-0.159967303276062,
0.03931073844432831,
0.07439126819372177,
-0.015527628362178802,
0.03031245246529579,
-0.09495621919631958,
0.03529537096619606,
0.042860399931669235,
-0.038158658891916275,
0.05061572790145874,
-0.0065283384174108505,
-0.011356830596923828,
0.025469904765486717,
0.01712634041905403,
0.057635121047496796,
-0.02035941183567047,
0.04955143854022026,
0.019876446574926376,
0.014519313350319862,
0.004965278785675764,
0.017284000292420387,
0.021647587418556213,
-0.062329865992069244,
0.020718874409794807,
0.07286443561315536,
0.028468027710914612,
0.013675393536686897,
0.08280225843191147,
0.01255439780652523,
-0.049198806285858154,
-0.052146147936582565,
-0.025028478354215622,
-0.08511226624250412,
0.030193306505680084,
0.03719743713736534,
0.012256323359906673,
0.01767420768737793,
0.39830857515335083,
-0.018812838941812515,
0.02991386502981186,
0.007496323902159929,
-0.02086423523724079,
-0.010405843146145344,
-0.03656011447310448,
-0.059372078627347946,
0.042821962386369705,
0.020595310255885124,
0.013020705431699753,
0.08863649517297745,
-0.010195945389568806,
0.012709399685263634,
-0.029802100732922554,
-0.004701468162238598,
-0.0022380268201231956,
-0.03846209496259689,
0.0123325539752841,
-0.05229903385043144,
0.059862300753593445,
-0.006787281949073076,
0.02108822390437126,
0.025065813213586807,
0.029820837080478668,
0.06412328034639359,
0.027303796261548996,
-0.06532113254070282,
0.08906783908605576,
0.042197953909635544,
-0.003624525386840105,
0.013315651565790176,
0.019273629412055016,
-0.02363935299217701,
0.02014891244471073,
-0.01675935462117195,
-0.03168405219912529,
0.04826502129435539,
-0.033800430595874786,
0.0559404082596302,
-0.027968162670731544,
0.014719432219862938,
0.02798016369342804,
-0.03198783099651337,
-0.028217855840921402,
-0.016465289518237114,
0.039340756833553314,
-0.00785746704787016,
0.037934593856334686,
-0.002955493051558733,
0.024657348170876503,
0.01525250542908907,
0.061857156455516815,
-0.06887678056955338,
0.009281475096940994,
0.02849806658923626,
-0.02076934650540352,
0.01752924732863903,
-0.0140461390838027,
0.03360232338309288,
-0.024227432906627655,
-0.0901477187871933,
0.009110590443015099,
0.03587832301855087,
0.08947869390249252,
-0.010811532847583294,
-0.05250812694430351,
-0.02368096448481083,
0.03161650896072388,
-0.05629243701696396,
-0.01110147126019001,
0.04288264364004135,
0.0013158578658476472,
0.007979595102369785,
-0.021292991936206818,
-0.04200223833322525,
-0.03288130834698677,
0.0012984012719243765,
0.03731786832213402,
0.03432558476924896,
0.011472707614302635,
0.0002875927893910557,
0.02341287024319172,
-0.028105437755584717,
0.01977711357176304,
0.045573581010103226,
-0.019135601818561554,
0.021759720519185066,
0.006361158564686775,
-0.02588900551199913,
-0.0017007156275212765,
-0.002553314669057727,
0.0339449979364872,
-0.09354794025421143,
0.0397663414478302,
-0.016031067818403244,
-0.04790179803967476,
-0.04495462775230408,
0.07744353264570236,
0.01994292438030243,
-0.04780378192663193,
0.09197482466697693,
0.020406609401106834,
0.02693766914308071,
0.010077468119561672,
-0.0414494089782238,
0.036937180906534195,
-0.03611370548605919,
0.03998706862330437,
-0.0046839225105941296,
0.034355271607637405,
-0.012797627598047256,
0.029488718137145042,
-0.06654198467731476,
0.0043802508153021336,
-0.05997255817055702,
0.08603127300739288,
0.0002974613453261554,
0.008241107687354088,
0.03489437699317932,
0.01565397158265114,
-0.032062631100416183,
-0.06248361989855766,
-0.0860653892159462,
-0.2795434594154358,
-0.017294785007834435,
0.04219165816903114,
-0.008801215328276157,
0.048298634588718414,
-0.0016938912449404597,
0.00341914058662951,
-0.012986398302018642,
0.02241402305662632,
-0.055750712752342224,
0.04515443742275238,
0.05862627178430557,
-0.03687633201479912,
-0.018285520374774933,
-0.02822716347873211,
0.02122947946190834,
0.030425632372498512,
-0.04193279892206192,
-0.014483737759292126,
-0.00974769052118063,
0.03267469257116318,
0.04027228802442551,
-0.003115725703537464,
0.014345521107316017,
0.013494263403117657,
-0.02039939910173416,
0.05433143302798271,
-0.03126845881342888,
0.05938978120684624,
-0.02148769609630108,
0.06430614739656448,
0.02322523668408394,
0.03439871966838837,
-0.07583358138799667,
-0.08277694135904312,
0.005333387292921543,
-0.037826646119356155,
-0.07278040051460266,
0.006748317740857601,
0.0003642998926807195,
0.03146171197295189,
-0.04122228920459747,
-0.030125288292765617,
-0.04297051206231117,
-0.0025307454634457827,
-0.06970193982124329,
0.004177888855338097,
-0.007641754578799009,
0.048251524567604065,
-0.002235851716250181,
0.031350668519735336,
0.0345638208091259,
0.04731639102101326,
-0.04698479548096657,
-0.03466680273413658,
-0.03313499689102173,
-0.05414952337741852,
0.01103435643017292,
-0.08204073458909988,
-0.015891121700406075,
-0.02315937913954258,
-0.03133181110024452,
-0.001996473642066121,
0.056519921869039536,
-0.057767536491155624,
-0.0453549399971962,
0.017909472808241844,
-0.018872881308197975,
0.05924380570650101,
0.016753744333982468,
0.00461352476850152,
0.008848101831972599,
-0.05135972797870636,
-0.020242763683199883,
-0.010239802300930023,
0.018604597076773643,
-0.05356340855360031,
-0.04925769194960594,
-0.07183297723531723,
-0.0346309058368206,
0.009378698654472828,
-0.03072422556579113,
0.1134130209684372,
-0.009611175395548344,
-0.005425199866294861,
0.09830876439809799,
-0.025538861751556396,
0.012080565094947815,
0.028702812269330025,
0.01803901046514511,
0.05804213508963585,
-0.05602211132645607,
0.013088946230709553,
-0.054155364632606506,
0.06995300203561783,
0.012770318426191807,
-0.2600918710231781,
-0.023507721722126007,
0.01750529184937477,
0.06913968920707703,
-0.03907904028892517,
-0.0040627093985676765,
0.04513373598456383,
-0.04536423459649086,
0.03628037869930267,
0.05145952105522156,
0.02376023679971695,
0.04377400502562523,
0.0023505871649831533,
0.05659560114145279,
0.031075647100806236,
0.002408755011856556,
0.01550421491265297,
-0.008685335516929626,
0.0000361109159712214,
-0.02809237502515316,
-0.02361319214105606,
-0.05194487050175667,
0.11965882033109665,
-0.028889605775475502,
-0.013300972059369087,
0.04778563976287842,
0.06279874593019485,
0.02207905985414982,
-0.06857195496559143,
0.02541307546198368,
0.002419606316834688,
0.027215609326958656,
0.10788265615701675,
-0.01171568688005209,
0.014064891263842583,
0.0388091504573822,
0.03349440172314644,
-0.00993857067078352,
-0.0034816106781363487,
0.04518746957182884,
-0.060883909463882446,
0.0021177411545068026,
-0.069259874522686,
-0.02007468231022358,
0.05732235684990883,
-0.08056314289569855,
-0.024594131857156754,
-0.05178876221179962,
-0.035702429711818695,
0.02299140766263008,
-0.012921386398375034,
0.00515028927475214,
-0.010389507748186588,
-0.009306167252361774,
0.01597742736339569,
0.039339423179626465,
-0.05516134575009346,
-0.03271409124135971,
-0.058238767087459564,
0.007511933334171772,
0.012042422778904438,
-0.00126864411868155,
-0.01171362679451704,
0.12008393555879593,
-0.011243660934269428
] |
PMID:8316 | Interest of isoproterenol in the treatment of hemorrhagic shock in dogs. | Four groups of experiments were focused on two problems: first, the replacement of the substracted amount of blood, and second, the correction of the peripheral reactions to substantial blood loss, namely vasoconstriction and acidosis. We stuck to a simple plan in our experiments: lost blood was entirely collected and retransfused. In 26 cases out of 37, we used isoproterenol hydrochloride (Isuprel - WINTHROP) to open the peripheral vascular bed. In six different groups, we followed the response to variations of retranfusion and administration of isoproterenol. Several parameters were studied: arterial blood pressure, central venous pressure, rectal temperature and pH. The association of blood transfusion with injection of isoproterenol, in adequate amounts to correct hypovolemia and to prevent vasoconstriction, is undoubtedly the best treatment of hemorrhagic shock. | Interest of isoproterenol in the treatment of hemorrhagic shock in dogs. Four groups of experiments were focused on two problems: first, the replacement of the substracted amount of blood, and second, the correction of the peripheral reactions to substantial blood loss, namely vasoconstriction and acidosis. We stuck to a simple plan in our experiments: lost blood was entirely collected and retransfused. In 26 cases out of 37, we used isoproterenol hydrochloride (Isuprel - WINTHROP) to open the peripheral vascular bed. In six different groups, we followed the response to variations of retranfusion and administration of isoproterenol. Several parameters were studied: arterial blood pressure, central venous pressure, rectal temperature and pH. The association of blood transfusion with injection of isoproterenol, in adequate amounts to correct hypovolemia and to prevent vasoconstriction, is undoubtedly the best treatment of hemorrhagic shock. | [
-0.06825375556945801,
-0.0041517335921525955,
-0.04515290632843971,
0.039610713720321655,
0.014955740422010422,
-0.011068841442465782,
-0.01737484522163868,
0.07780899852514267,
-0.0455162487924099,
-0.033255014568567276,
0.045611925423145294,
-0.046749718487262726,
0.0006290578166954219,
0.07042325288057327,
-0.062184810638427734,
0.023452943190932274,
0.05304619297385216,
0.018860185518860817,
-0.08610370755195618,
-0.006984235718846321,
-0.02239360101521015,
-0.024850402027368546,
0.02301243506371975,
0.008636625483632088,
0.027472486719489098,
-0.00833673682063818,
0.0025014448910951614,
-0.013583037070930004,
-0.051244258880615234,
-0.15675728023052216,
0.02011348307132721,
-0.036555029451847076,
-0.0068679023534059525,
0.005765820853412151,
-0.04558105021715164,
0.04008445888757706,
-0.023488841950893402,
0.0054999953135848045,
-0.010706441476941109,
0.051137473434209824,
0.028837155550718307,
0.006424763705581427,
-0.03202063962817192,
0.010825022123754025,
0.04253174737095833,
0.021624885499477386,
0.0053480882197618484,
0.002048355992883444,
0.14411389827728271,
0.02239605411887169,
0.05349525436758995,
0.03543638065457344,
0.01811336725950241,
0.0244070366024971,
0.023263361304998398,
-0.035725340247154236,
0.037493202835321426,
0.032875318080186844,
0.004675533622503281,
0.017764750868082047,
0.04751388728618622,
0.05491845682263374,
-0.12427403032779694,
0.08558445423841476,
0.003394093830138445,
0.03968219459056854,
-0.04908483847975731,
0.007258826866745949,
0.06144464388489723,
0.06612846255302429,
-0.04049011692404747,
0.029544949531555176,
0.023784352466464043,
-0.008660169318318367,
-0.03553649038076401,
-0.019514700397849083,
0.03012029640376568,
-0.03815755620598793,
-0.002789200283586979,
-0.019155502319335938,
-0.00527225760743022,
0.005465204361826181,
-0.038110267370939255,
-0.022038983181118965,
0.03412787243723869,
-0.07544763386249542,
0.07164373248815536,
-0.0070306663401424885,
-0.01915871351957321,
-0.030089089646935463,
-0.0559871681034565,
-0.05521330237388611,
-0.023966830223798752,
-0.016079701483249664,
-0.040670245885849,
0.008939228020608425,
-0.02766256034374237,
-0.008224953897297382,
0.018856419250369072,
0.3494427800178528,
-0.004523873329162598,
0.0080250333994627,
-0.05901433527469635,
-0.03829400986433029,
0.042656902223825455,
-0.0688026174902916,
0.01978633925318718,
0.05395307019352913,
-0.047569893300533295,
0.06874041259288788,
0.021938424557447433,
0.03754321485757828,
0.08807117491960526,
-0.036190345883369446,
-0.02430804632604122,
0.019608614966273308,
0.010545894503593445,
-0.02012573555111885,
-0.03136236220598221,
0.03624694421887398,
-0.018178608268499374,
0.045772843062877655,
-0.0030522141605615616,
-0.020289206877350807,
0.046377070248126984,
-0.0038079400546848774,
0.05358382314443588,
0.04330964758992195,
0.02397630177438259,
0.010504044592380524,
0.04824827238917351,
0.04197971522808075,
-0.009346297942101955,
-0.001454101875424385,
-0.033588752150535583,
-0.007753306534141302,
0.04395813122391701,
0.010335070081055164,
0.058020636439323425,
0.04902537912130356,
-0.004212741740047932,
-0.024888718500733376,
-0.037070516496896744,
-0.07071176171302795,
-0.04882194101810455,
0.0681900680065155,
0.01425349060446024,
-0.02318245731294155,
-0.04433566331863403,
-0.003577875206246972,
-0.04529643431305885,
-0.0026229883078485727,
-0.04400324076414108,
0.040186524391174316,
-0.03904949873685837,
0.012638856656849384,
-0.038148362189531326,
-0.00630251644179225,
-0.0413491427898407,
-0.023979946970939636,
-0.02428983710706234,
-0.047272928059101105,
-0.05560795217752457,
0.02972632460296154,
-0.035459958016872406,
-0.018031679093837738,
-0.04767628386616707,
-0.04901980608701706,
0.03546189144253731,
0.05179582163691521,
-0.037443555891513824,
0.0096857575699687,
0.04040471464395523,
-0.05080687254667282,
-0.0252702459692955,
0.02965598739683628,
-0.005393388215452433,
-0.04576324298977852,
0.0663628950715065,
-0.055053699761629105,
0.03562226518988609,
0.007074484135955572,
0.028485842049121857,
0.06286841630935669,
-0.012111249379813671,
0.029556063935160637,
-0.035367295145988464,
-0.033027276396751404,
-0.012048350647091866,
0.08256938308477402,
-0.012552540749311447,
0.012170271016657352,
-0.030173758044838905,
0.014277138747274876,
0.012374897487461567,
0.04207555577158928,
-0.0891190841794014,
-0.021034585312008858,
-0.06793109327554703,
0.025785868987441063,
0.10779600590467453,
-0.010919163934886456,
0.00990087166428566,
0.03229650855064392,
0.08578228205442429,
0.09676583111286163,
0.012603607028722763,
-0.011178325861692429,
0.0435255691409111,
-0.005156723782420158,
0.027318332344293594,
0.07313691824674606,
0.02899077720940113,
0.007528426591306925,
-0.04150691255927086,
0.06279762834310532,
-0.010905756615102291,
0.02549775503575802,
0.07053644955158234,
0.03126364201307297,
0.07508645206689835,
-0.0605284608900547,
0.013072383590042591,
-0.3409637212753296,
-0.06573068350553513,
-0.029637372121214867,
-0.027674168348312378,
0.06984410434961319,
-0.025398217141628265,
0.046938423067331314,
-0.07655517011880875,
-0.05694970116019249,
-0.002921098144724965,
0.030347734689712524,
0.024045368656516075,
-0.021536676213145256,
-0.04383513703942299,
0.024554217234253883,
0.05846699699759483,
0.061448223888874054,
-0.07043378800153732,
-0.028756918385624886,
-0.031394824385643005,
0.0324346199631691,
-0.022362127900123596,
-0.013729888945817947,
0.048139527440071106,
0.008614501915872097,
-0.004010852426290512,
0.1148456484079361,
-0.05695235729217529,
0.005087161436676979,
-0.035498034209012985,
-0.05854783579707146,
-0.014388599433004856,
0.017912546172738075,
-0.07971888035535812,
0.01179034635424614,
-0.06796009093523026,
0.03869638592004776,
-0.04781694710254669,
0.06249851733446121,
-0.017642350867390633,
-0.01662728562951088,
0.027203209698200226,
0.009406689554452896,
-0.013527978211641312,
-0.06109292805194855,
0.06393685191869736,
0.00023728562518954277,
0.019732415676116943,
-0.021277474239468575,
0.07548349350690842,
0.02889135666191578,
0.022842422127723694,
-0.010387402027845383,
0.03362572565674782,
0.06742934137582779,
0.00796038843691349,
-0.05457661673426628,
-0.00856766663491726,
-0.07674190402030945,
-0.013509921729564667,
0.027919931337237358,
0.002482442418113351,
0.03613104298710823,
-0.025421245023608208,
-0.013434049673378468,
-0.013300111517310143,
0.07793055474758148,
0.03970242291688919,
0.09137019515037537,
0.0459606871008873,
-0.026504239067435265,
0.09711526334285736,
-0.05717357620596886,
0.018076594918966293,
0.0002955759991891682,
0.013520607724785805,
-0.03478603810071945,
-0.029079828411340714,
-0.05462709069252014,
-0.018987851217389107,
0.043631572276353836,
-0.08815347403287888,
0.04429466649889946,
-0.008598671294748783,
0.01885261945426464,
-0.0033687688410282135,
-0.04501352831721306,
-0.00931563414633274,
0.03790750727057457,
-0.040856700390577316,
0.0022805461194366217,
-0.04814424365758896,
-0.06167967990040779,
-0.00006379649130394682,
-0.005538917146623135,
0.04856399446725845,
-0.26410600543022156,
-0.012364047579467297,
0.012790491804480553,
-0.07657282054424286,
-0.025879506021738052,
-0.024985050782561302,
6.14952966770943e-7,
-0.0769159197807312,
0.03378509357571602,
0.003828518558293581,
0.028686247766017914,
-0.014142694883048534,
0.02951117977499962,
0.0437341146171093,
-0.007967616431415081,
-0.02668118290603161,
0.06513433903455734,
0.005065514240413904,
-0.011603468097746372,
-0.055916015058755875,
-0.058028172701597214,
-0.0319954976439476,
0.15253016352653503,
-0.025746049359440804,
-0.010551925748586655,
0.052004631608724594,
0.013401343487203121,
0.0320742130279541,
-0.014740260317921638,
0.030381303280591965,
-0.01755538582801819,
-0.011377394199371338,
0.038392916321754456,
-0.024284327402710915,
0.022746257483959198,
-0.009946117177605629,
-0.03465183824300766,
0.025565998628735542,
0.045358411967754364,
0.012216676957905293,
-0.030354326590895653,
0.03383440524339676,
-0.00910321343690157,
-0.038427334278821945,
0.07053378224372864,
-0.03943537920713425,
-0.024003660306334496,
-0.020166324451565742,
-0.05588090792298317,
0.024971645325422287,
-0.06409025937318802,
-0.0011271906550973654,
0.06827276945114136,
0.006300560664385557,
-0.0055953278206288815,
-0.007661964278668165,
-0.03938790410757065,
-0.028439577668905258,
-0.000509021629113704,
-0.02840553969144821,
0.01642625778913498,
-0.011103363707661629,
-0.033924270421266556,
0.07350178062915802,
0.026901941746473312
] |
PMID:8317 | The noradrenergic cyclic AMP generating system in the limbic forebrain: pharmacological characterization in vitro and possible role of limbic noradrenergic mechanisms in the mode of action of antipsychotics. | The cyclic AMP generating system in slices of the rat limbic forebrain was investigated. In consists of: (u) A noradrenergic system which responds to norepinephrine (NE) and isoproterenol. Though the rise of the nucleotide elicited by isoproterenol is more rapid than that caused by NE, the maximal effect is less than half of that induced by NE; (2) an adenosine-dependent system. The noradrenergic cyclic AMP generating system in the limbic forebrain displays a number of properties of a central NE receptor: it develops supersensitivity to NE and isoproterenol following prolonged deprivation of NE at postsynaptic sites (chronic treatment with reserpine or chemosympathectomy with 6-hydroxydopamine). When noradrenergic terminals are protected from 6-hydroxydopamine by desmethylimipramine, the responses to NE are not enhanced. Responses to NE are blocked by both propranolol and phentolamine, while responses to isoproterenol are blocked by propranolol but not by phentolamine. The adenosine-dependent system does not develop supersensitivity after central chemosympathectomy and is not blocked by either alpha- or beta-antagonists. While not altering the basal level of the nucleotide, clinically effective antipsychotic drugs caused a dose-dependent inhibition of the limbic noradrenergic cyclic AMP response with clozapine and pimozide being particularly potent (IC50 0.06 and 0.08 muM, respectively). Antipsychotic drugs do, however, not affect cyclic AMP responses elicited by adenosine. The results are compatible with the view that the central NE receptor is closely related to or may be an integral part of an adenylate cyclase system and that its blockade in the limbic forebrain by antipsychotic drugs may contribute to their therapeutic action. | The noradrenergic cyclic AMP generating system in the limbic forebrain: pharmacological characterization in vitro and possible role of limbic noradrenergic mechanisms in the mode of action of antipsychotics. The cyclic AMP generating system in slices of the rat limbic forebrain was investigated. In consists of: (u) A noradrenergic system which responds to norepinephrine (NE) and isoproterenol. Though the rise of the nucleotide elicited by isoproterenol is more rapid than that caused by NE, the maximal effect is less than half of that induced by NE; (2) an adenosine-dependent system. The noradrenergic cyclic AMP generating system in the limbic forebrain displays a number of properties of a central NE receptor: it develops supersensitivity to NE and isoproterenol following prolonged deprivation of NE at postsynaptic sites (chronic treatment with reserpine or chemosympathectomy with 6-hydroxydopamine). When noradrenergic terminals are protected from 6-hydroxydopamine by desmethylimipramine, the responses to NE are not enhanced. Responses to NE are blocked by both propranolol and phentolamine, while responses to isoproterenol are blocked by propranolol but not by phentolamine. The adenosine-dependent system does not develop supersensitivity after central chemosympathectomy and is not blocked by either alpha- or beta-antagonists. While not altering the basal level of the nucleotide, clinically effective antipsychotic drugs caused a dose-dependent inhibition of the limbic noradrenergic cyclic AMP response with clozapine and pimozide being particularly potent (IC50 0.06 and 0.08 muM, respectively). Antipsychotic drugs do, however, not affect cyclic AMP responses elicited by adenosine. The results are compatible with the view that the central NE receptor is closely related to or may be an integral part of an adenylate cyclase system and that its blockade in the limbic forebrain by antipsychotic drugs may contribute to their therapeutic action. | [
-0.029986657202243805,
0.004037722013890743,
-0.01392440777271986,
0.041007522493600845,
0.025695068761706352,
0.044111739844083786,
0.043146222829818726,
0.036679428070783615,
-0.014095224440097809,
-0.048187412321567535,
0.027851106598973274,
-0.010500848293304443,
-0.012129278853535652,
0.05633297935128212,
-0.041658252477645874,
-0.02757464163005352,
0.03726603090763092,
0.021636802703142166,
0.01563880406320095,
-0.04065808653831482,
-0.002045517321676016,
-0.02776481769979,
0.04579957574605942,
-0.006496809888631105,
-0.02505805902183056,
-0.0010550243314355612,
0.006088768132030964,
-0.005402573384344578,
-0.04769829660654068,
-0.20098091661930084,
0.05602002516388893,
0.03566911444067955,
0.020312117412686348,
-0.044209979474544525,
-0.03892363980412483,
-0.03585684299468994,
-0.010632560588419437,
0.06193464249372482,
-0.026666274294257164,
0.032773517072200775,
0.05100677162408829,
0.011676949448883533,
0.02831512875854969,
0.015959637239575386,
0.05220269039273262,
-0.02574431151151657,
-0.044232554733753204,
-0.0663369819521904,
0.1020914614200592,
-0.04298165813088417,
0.028668798506259918,
0.005140849854797125,
0.025105668231844902,
0.06496008485555649,
0.020109431818127632,
0.024043915793299675,
-0.03728273883461952,
0.0670350044965744,
-0.03457824885845184,
0.001639976049773395,
-0.008217668160796165,
0.03398292884230614,
-0.13205917179584503,
0.03424752876162529,
0.03954481706023216,
0.013360516168177128,
-0.0030608063098043203,
-0.0010486709652468562,
0.00944586843252182,
0.020225849002599716,
-0.04198887199163437,
0.019246751442551613,
-0.03523170202970505,
-0.02889668196439743,
0.016753265634179115,
-0.0002714133297558874,
-0.0173052866011858,
-0.024323729798197746,
0.048637233674526215,
-0.07452712208032608,
0.035919055342674255,
-0.007410313002765179,
-0.009287367574870586,
-0.052138552069664,
0.0406988188624382,
-0.014899089932441711,
0.017777888104319572,
-0.048647139221429825,
-0.031234268099069595,
-0.006762600503861904,
-0.07477822154760361,
-0.031019482761621475,
0.04465098679065704,
-0.03174319863319397,
-0.024415239691734314,
0.009144317358732224,
-0.012200750410556793,
-0.0510893315076828,
-0.00921467412263155,
0.3438911736011505,
0.021631279960274696,
0.012186260893940926,
-0.024929413571953773,
-0.04000532254576683,
-0.030924970284104347,
-0.04323528707027435,
-0.023098593577742577,
0.005338476970791817,
-0.04781261458992958,
0.07788988947868347,
0.02269519492983818,
0.004965027328580618,
0.04014775902032852,
-0.0939430221915245,
-0.01892627775669098,
-0.057960666716098785,
0.05373194068670273,
-0.04362634941935539,
0.025402411818504333,
0.017767218872904778,
-0.04956890270113945,
-0.005809862166643143,
0.0017848958959802985,
0.01027220394462347,
0.04594101011753082,
-0.015641916543245316,
-0.033910121768713,
0.08627241849899292,
0.08030468225479126,
-0.03075602278113365,
0.07334208488464355,
0.04949516803026199,
-0.020526064559817314,
0.034553952515125275,
0.02986086718738079,
-0.03349709138274193,
0.018848000094294548,
-0.022527208551764488,
0.03191615641117096,
-0.015054128132760525,
0.008441842161118984,
-0.04304671660065651,
0.02762146294116974,
-0.07377930730581284,
-0.04622148722410202,
0.07523029297590256,
-0.025059310719370842,
-0.03161703795194626,
-0.045994777232408524,
0.07954412698745728,
-0.049340154975652695,
0.06838256120681763,
-0.028380997478961945,
0.030795186758041382,
-0.020167922601103783,
0.06723076850175858,
-0.028205491602420807,
-0.007983178831636906,
-0.020921839401125908,
-0.018627719953656197,
-0.0997384563088417,
-0.03783644363284111,
0.018398350104689598,
0.09400936216115952,
0.041430167853832245,
-0.0010193433845415711,
-0.03380845859646797,
-0.006748958490788937,
-0.009352452121675014,
-0.020521927624940872,
-0.014341006986796856,
0.026165055111050606,
0.003929763101041317,
-0.03744601085782051,
-0.049991048872470856,
-0.015390567481517792,
0.03521846979856491,
0.006832195911556482,
0.01100863516330719,
-0.056584130972623825,
0.0037819892168045044,
-0.02961346134543419,
0.0033840255346149206,
0.028084751218557358,
-0.0005666963406838477,
0.038218602538108826,
-0.05296836793422699,
-0.02344869077205658,
-0.013243595138192177,
0.04479880630970001,
-0.015402810648083687,
0.02789306826889515,
-0.1139756441116333,
0.031438082456588745,
-0.07295947521924973,
-0.061835482716560364,
-0.024036426097154617,
0.008249089121818542,
-0.03130052238702774,
0.048241548240184784,
0.06748031079769135,
0.041369881480932236,
0.0031558184418827295,
0.05375711992383003,
-0.01051250658929348,
0.028344381600618362,
-0.022352438420057297,
0.05489400774240494,
0.07599209994077682,
0.037818148732185364,
0.02183946594595909,
-0.01441207341849804,
0.09272562712430954,
-0.0036179805174469948,
-0.04799896851181984,
0.08285107463598251,
-0.04913854971528053,
0.03236860781908035,
0.09918248653411865,
0.011335671879351139,
0.000352762290276587,
0.005319204181432724,
0.0036016216035932302,
-0.29371264576911926,
-0.000310896139126271,
-0.021528249606490135,
-0.017256075516343117,
0.04831709712743759,
-0.02140709199011326,
0.06181304529309273,
-0.0688963532447815,
0.024148648604750633,
-0.060604773461818695,
-0.0015823792200535536,
0.06591635942459106,
-0.040358785539865494,
-0.006495398003607988,
-0.02381793223321438,
0.08090104907751083,
0.0008091565105132759,
-0.04641228914260864,
0.0075895171612501144,
0.017282634973526,
0.05903223156929016,
-0.028079863637685776,
-0.010816719383001328,
0.0007123868563212454,
-0.005487109534442425,
-0.02931581623852253,
0.0847066268324852,
0.036383237689733505,
0.023962968960404396,
-0.010543673299252987,
-0.0242809746414423,
0.045042842626571655,
-0.03484508395195007,
-0.08584967255592346,
-0.022456014528870583,
-0.028239592909812927,
0.01619710586965084,
0.02211371436715126,
0.023500237613916397,
0.025533469393849373,
-0.05524776875972748,
0.04015004262328148,
-0.005597192328423262,
0.016733871772885323,
-0.06473565101623535,
-0.011271029710769653,
0.012760583311319351,
0.0625721886754036,
0.05571437627077103,
-0.005332046188414097,
-0.003804206382483244,
0.012208975851535797,
-0.01977827027440071,
-0.045446235686540604,
0.035431187599897385,
-0.022467708215117455,
-0.06665557622909546,
-0.025082990527153015,
-0.06496413797140121,
0.015643294900655746,
0.029515178874135017,
0.03831404075026512,
-0.007119505666196346,
-0.03759736940264702,
-0.05051951855421066,
0.07115825265645981,
0.028273168951272964,
0.001994369551539421,
0.04819949343800545,
-0.005296876188367605,
0.03226955235004425,
0.12789033353328705,
-0.030069902539253235,
0.04297703132033348,
0.052358321845531464,
-0.00543170003220439,
-0.05947069078683853,
-0.07100305706262589,
-0.048262909054756165,
0.03905152529478073,
-0.009988519363105297,
-0.058981094509363174,
0.07887688279151917,
0.03790393844246864,
-0.07113537192344666,
-0.040429532527923584,
0.04768975451588631,
-0.018823228776454926,
0.07102696597576141,
-0.03386722505092621,
0.014316875487565994,
0.06817220896482468,
0.006471211556345224,
-0.05229167640209198,
0.05004750192165375,
0.06863800436258316,
-0.2802606225013733,
-0.03100939281284809,
-0.008063871413469315,
-0.05649290233850479,
0.024700822308659554,
0.03515945374965668,
0.00944332592189312,
-0.0190956499427557,
-0.0349101796746254,
0.030781935900449753,
0.005891707725822926,
0.02678230032324791,
0.041479550302028656,
0.08597609400749207,
0.00618024030700326,
0.04016914218664169,
0.05003528669476509,
-0.03157803416252136,
0.04492538794875145,
-0.05712905153632164,
0.02322043478488922,
0.015516208484768867,
0.1522650122642517,
0.010267809964716434,
-0.003559263190254569,
0.0005692478152923286,
-0.02417716570198536,
0.022028259932994843,
-0.008525167591869831,
0.030930496752262115,
-0.0628555566072464,
0.03871225193142891,
0.03913767263293266,
-0.026673851534724236,
0.019832752645015717,
0.02095194160938263,
-0.05161748081445694,
-0.0034470746759325266,
0.04261533543467522,
-0.04485125094652176,
-0.04810790717601776,
0.04378369078040123,
-0.05502967908978462,
-0.010899380780756474,
0.09130454063415527,
-0.10386064648628235,
-0.0579051710665226,
-0.007066910155117512,
-0.01499730721116066,
0.0309462770819664,
-0.003870202461257577,
-0.004768007900565863,
0.029967447742819786,
0.0170414000749588,
0.005141168367117643,
-0.05968073010444641,
-0.0572691485285759,
-0.03962644189596176,
0.007035819347947836,
-0.01781359128654003,
-0.05688363313674927,
0.003472455544397235,
0.022825967520475388,
0.058308154344558716,
0.022551577538251877
] |
PMID:8318 | Effect of propranolol of vascular responses to sympathetic nerve stimulation and plasma renin activity in mongrel dogs. | I.v administration of propranolol (0.2 mg/kg and 1.0 mg/kg) to pentobarbital-anesthetized dogs produced blockade of cardiac beta-receptors and a significant decrease in heart rate. However, only the higher dose of propranolol demonstrated a significant hypotensive effect. Furthermore, this hypotensive action of propranolol was not associated with either adrenergic neruron blockade or changes in plasma renin activity. These results indicate that the initial hypotensive action of propranolol in mongrel dogs is not due to the blockade of beta-receptors, alterations in peripheral sympathetic nervous transmission or plasma renin activity. On the other hand, the action that propranolol is reported to have within the central system may play an important role in accounting for the acute blood pressure lowering action of the compound in mongrel dogs. | Effect of propranolol of vascular responses to sympathetic nerve stimulation and plasma renin activity in mongrel dogs. I.v administration of propranolol (0.2 mg/kg and 1.0 mg/kg) to pentobarbital-anesthetized dogs produced blockade of cardiac beta-receptors and a significant decrease in heart rate. However, only the higher dose of propranolol demonstrated a significant hypotensive effect. Furthermore, this hypotensive action of propranolol was not associated with either adrenergic neruron blockade or changes in plasma renin activity. These results indicate that the initial hypotensive action of propranolol in mongrel dogs is not due to the blockade of beta-receptors, alterations in peripheral sympathetic nervous transmission or plasma renin activity. On the other hand, the action that propranolol is reported to have within the central system may play an important role in accounting for the acute blood pressure lowering action of the compound in mongrel dogs. | [
-0.05462973937392235,
-0.010750608518719673,
-0.005345399025827646,
0.01628650724887848,
-0.0016551067819818854,
-0.034846868366003036,
-0.00012470509682316333,
0.052161943167448044,
-0.022731587290763855,
-0.03551776707172394,
-0.0019542223308235407,
-0.015633229166269302,
0.010347620584070683,
0.0198321845382452,
-0.08526740223169327,
-0.057617805898189545,
0.0622236393392086,
-0.016993489116430283,
-0.07434090226888657,
-0.006706487387418747,
-0.0403437577188015,
-0.016794370487332344,
0.01503551285713911,
0.04960976168513298,
-0.0012442013248801231,
0.02745898813009262,
-0.035813603550195694,
-0.0021465225145220757,
-0.03518537059426308,
-0.16345052421092987,
0.025944793596863747,
-0.001388923148624599,
0.02780357003211975,
-0.007374780718237162,
-0.035686712712049484,
0.010453938506543636,
-0.03407258167862892,
0.03845049440860748,
-0.009761362336575985,
0.058092936873435974,
0.06167364493012428,
-0.07074280828237534,
0.022883864119648933,
0.04006730020046234,
0.011173575185239315,
0.020659087225794792,
-0.022698933258652687,
-0.01467821840196848,
0.10749024152755737,
0.0013202769914641976,
0.023895418271422386,
-0.00782956276088953,
0.009706495329737663,
0.06547775864601135,
0.035197075456380844,
-0.02258451282978058,
-0.025727128610014915,
0.060404252260923386,
0.025999264791607857,
0.027175383642315865,
0.008744600228965282,
0.04777282476425171,
-0.18680357933044434,
0.06171967834234238,
0.02840672805905342,
0.054119907319545746,
-0.017776207998394966,
0.050762370228767395,
0.03758622705936432,
0.04025997221469879,
0.019825737923383713,
0.04724232479929924,
-0.009213367477059364,
-0.0411587730050087,
-0.01021958515048027,
-0.07984310388565063,
-0.02537100948393345,
0.03745383024215698,
-0.02431129291653633,
-0.009679383598268032,
0.05184153839945793,
-0.010579690337181091,
-0.035447392612695694,
-0.020047269761562347,
0.06879619508981705,
-0.07753881067037582,
0.08600139617919922,
-0.04249613732099533,
-0.015227056108415127,
-0.04567578062415123,
-0.0682893842458725,
-0.04953081160783768,
-0.010148783214390278,
0.0056436918675899506,
-0.007050978951156139,
0.058175139129161835,
-0.017694996669888496,
-0.0395585261285305,
-0.04185517132282257,
0.33390623331069946,
0.0013110802974551916,
0.049663301557302475,
-0.09381755441427231,
0.00026902512763626873,
0.08122999221086502,
-0.03751283511519432,
0.036371614784002304,
0.03813151270151138,
-0.033305004239082336,
0.043785423040390015,
0.04140859097242355,
0.016741830855607986,
0.04744357988238335,
-0.09577677398920059,
-0.01336855348199606,
-0.056406646966934204,
0.03717731684446335,
0.011954234912991524,
0.02606128714978695,
0.03190965577960014,
-0.028557803481817245,
0.022896237671375275,
0.031677622348070145,
0.025879818946123123,
0.0579637810587883,
0.02301952987909317,
0.01552825141698122,
0.08518626540899277,
0.033755913376808167,
0.02434741146862507,
0.05011403560638428,
0.014640477485954762,
-0.03515953943133354,
-0.015635889023542404,
0.04790634289383888,
-0.012214179150760174,
0.0428127683699131,
-0.027927815914154053,
0.04005580395460129,
-0.02821323834359646,
0.013369753025472164,
-0.04878847301006317,
0.010386844165623188,
-0.06754504889249802,
-0.03368941694498062,
0.11216198652982712,
0.0654016062617302,
-0.026318222284317017,
-0.07890788465738297,
0.04230324923992157,
-0.07040325552225113,
0.02269725129008293,
-0.022586483508348465,
0.0565115250647068,
-0.041968800127506256,
0.011394481174647808,
-0.025331223383545876,
0.03252026066184044,
-0.023006781935691833,
-0.041245341300964355,
-0.04623440280556679,
-0.06044907867908478,
-0.01866617426276207,
-0.012455088086426258,
0.029688896611332893,
0.03638719767332077,
-0.04623425379395485,
0.013370453380048275,
0.03611389920115471,
0.02194817177951336,
-0.026251837611198425,
0.03514154255390167,
0.0057314191944897175,
-0.03305410221219063,
-0.03733748570084572,
0.008777101524174213,
0.02845957688987255,
-0.03543664515018463,
-0.011514679528772831,
-0.064522884786129,
0.011867492459714413,
0.04194523021578789,
0.04900054633617401,
0.0071498313918709755,
-0.04218170791864395,
0.057118453085422516,
-0.06102803349494934,
-0.006270339246839285,
-0.008434716612100601,
0.08430430293083191,
-0.024972205981612206,
-0.04127545654773712,
-0.07550624012947083,
-0.023199070245027542,
-0.04885655269026756,
0.005567356012761593,
-0.04552213475108147,
0.019350504502654076,
-0.11850117146968842,
0.008511626161634922,
0.0330527126789093,
0.0022543456871062517,
-0.011051548644900322,
0.02855994924902916,
0.04521932825446129,
0.09805917739868164,
-0.030749835073947906,
-0.019709467887878418,
0.0414700023829937,
-0.007898331619799137,
0.042036548256874084,
0.06955086439847946,
0.07366149127483368,
0.011721381917595863,
-0.04206523671746254,
0.02800731174647808,
-0.028238404542207718,
0.011018672026693821,
0.05770034343004227,
0.04416554421186447,
0.010217741131782532,
-0.03476865589618683,
0.03157874196767807,
-0.32699257135391235,
-0.061213940382003784,
-0.027109893038868904,
-0.03066590242087841,
0.03264231979846954,
-0.036667708307504654,
0.0354219451546669,
-0.025216398760676384,
-0.052961912006139755,
-0.040357284247875214,
0.05825778469443321,
0.03719695657491684,
0.03256182372570038,
-0.0016329278005287051,
0.03287015110254288,
0.04716285318136215,
0.0020762316416949034,
-0.02761148288846016,
-0.0038169040344655514,
-0.010490360669791698,
0.01680920273065567,
-0.037201669067144394,
-0.007961302064359188,
0.012823838740587234,
0.026284299790859222,
0.03621550276875496,
0.06047825515270233,
-0.020939944311976433,
-0.03727925941348076,
-0.000057674827985465527,
0.027905398979783058,
0.01629071868956089,
-0.008446875028312206,
-0.11280785501003265,
0.028451954945921898,
-0.05126001685857773,
0.05108286440372467,
0.04201623797416687,
0.002820550696924329,
0.005098425317555666,
-0.050150178372859955,
0.039891887456178665,
0.05145803093910217,
0.023547790944576263,
-0.024301765486598015,
0.04308215156197548,
0.049787696450948715,
0.09985388815402985,
-0.07472486048936844,
-0.004898820538073778,
-0.0054626502096652985,
0.00797441229224205,
-0.005528297740966082,
0.01330077275633812,
0.0887843668460846,
-0.009644393809139729,
-0.060234468430280685,
-0.033408429473638535,
-0.0328141413629055,
-0.008021236397325993,
0.044314127415418625,
0.005769161507487297,
0.038732193410396576,
-0.012964695692062378,
-0.052940595895051956,
0.04519747197628021,
-0.00871429406106472,
0.02221418172121048,
0.029593490064144135,
0.06223240867257118,
0.01732046902179718,
0.11328615993261337,
0.012150253169238567,
0.013751249760389328,
0.06900596618652344,
-0.01112123392522335,
-0.03968868777155876,
-0.05575636029243469,
-0.03888467699289322,
-0.010373066179454327,
-0.025889365002512932,
-0.10003750771284103,
0.0042754877358675,
-0.002179847564548254,
0.029878338798880577,
-0.0536370612680912,
0.05857521668076515,
0.011272926814854145,
0.04388734698295593,
-0.031536515802145004,
-0.036945492029190063,
-0.0016783035825937986,
-0.02545141614973545,
-0.04515545070171356,
-0.027943553403019905,
0.012148585170507431,
-0.22246108949184418,
-0.029504330828785896,
0.07261265814304352,
-0.05724926292896271,
0.03613513708114624,
-0.015968075022101402,
0.04107918590307236,
-0.07571762055158615,
-0.06959034502506256,
-0.0065653445199131966,
-0.04116654768586159,
0.05189292132854462,
0.07638174295425415,
0.06126319244503975,
-0.08705302327871323,
-0.022995317354798317,
0.028102386742830276,
-0.014842577278614044,
0.029663830995559692,
-0.053649578243494034,
0.01842888630926609,
-0.027930215001106262,
0.12181691080331802,
-0.023402348160743713,
0.013357038609683514,
0.06652706861495972,
-0.005369100719690323,
0.019573403522372246,
-0.041165124624967575,
0.022520441561937332,
-0.00979648344218731,
-0.027923982590436935,
0.0333380289375782,
-0.03430425375699997,
0.022155577316880226,
0.05737776681780815,
-0.06731171905994415,
-0.01001241710036993,
0.0178833045065403,
0.012113276869058609,
-0.009335174225270748,
0.028235672041773796,
-0.043652404099702835,
-0.040164969861507416,
0.07860874384641647,
-0.04277857020497322,
-0.008329630829393864,
0.007889915257692337,
0.02618817239999771,
0.014593865722417831,
-0.055531714111566544,
-0.014991511590778828,
0.06554874777793884,
0.04633982479572296,
-0.01932469755411148,
-0.048452071845531464,
-0.03385593742132187,
-0.042241793125867844,
-0.014038356952369213,
-0.032352522015571594,
0.016130931675434113,
-0.051378026604652405,
-0.0161557886749506,
0.02706732228398323,
0.04377291724085808
] |
PMID:8319 | Hypotensive responses following oral adminstration of beta-adrenoceptor blocking drugs to the conscious cat. | On oral administration, the non-selective beta-adrenoceptor blocking drugs (+/-)-bufuralol, (-)-bufuralol, propanolol, oxprenolol, pindolol and alprenolol produced hypotensive responses in the conscious cat; (+)-bufuralol was without effect. The selective beta-adrenoceptor blocking drugs practolol and atenolol had no effect on blood pressure but tolamolol elicited a hypotensive response. All the drugs tested reduced the tachycardia due to intravenous isoprenaline in the conscious cat; however, not all doses of these drugs reduced blood pressure. (+)-Bufuralol was devoid to beta-adrenoceptive blocking activity. Only tolamolol reduced the pressor response to i.v. phenylephrine in the conscious cat, indicating that alpha-adrenoceptive blocking activity may contribute to its hypotensive action. The results suggest that beta-adrenoceptive blocking activity is necessary for the hypotensive responses of these drugs. However, for the different drugs, there was no correlation between peripheral beta-adrenoceptive blocking activity and hypotensive response. | Hypotensive responses following oral adminstration of beta-adrenoceptor blocking drugs to the conscious cat. On oral administration, the non-selective beta-adrenoceptor blocking drugs (+/-)-bufuralol, (-)-bufuralol, propanolol, oxprenolol, pindolol and alprenolol produced hypotensive responses in the conscious cat; (+)-bufuralol was without effect. The selective beta-adrenoceptor blocking drugs practolol and atenolol had no effect on blood pressure but tolamolol elicited a hypotensive response. All the drugs tested reduced the tachycardia due to intravenous isoprenaline in the conscious cat; however, not all doses of these drugs reduced blood pressure. (+)-Bufuralol was devoid to beta-adrenoceptive blocking activity. Only tolamolol reduced the pressor response to i.v. phenylephrine in the conscious cat, indicating that alpha-adrenoceptive blocking activity may contribute to its hypotensive action. The results suggest that beta-adrenoceptive blocking activity is necessary for the hypotensive responses of these drugs. However, for the different drugs, there was no correlation between peripheral beta-adrenoceptive blocking activity and hypotensive response. | [
-0.06779670715332031,
0.03077133186161518,
-0.00303527619689703,
0.02976352907717228,
0.014796271920204163,
-0.002571054734289646,
0.017089053988456726,
0.018776020035147667,
-0.005249671172350645,
-0.0516219362616539,
-0.04949004203081131,
0.002251030644401908,
-0.009766479022800922,
0.062329936772584915,
-0.04061270132660866,
-0.022105718031525612,
0.05710957571864128,
-0.026400664821267128,
-0.03906012699007988,
-0.013702670112252235,
-0.01773618720471859,
0.014424195513129234,
0.012323151342570782,
0.03945918008685112,
0.010904080234467983,
-0.017591567710042,
-0.011912258341908455,
0.01310606300830841,
-0.06830141693353653,
-0.1613372564315796,
0.01918460801243782,
-0.03920896351337433,
-0.010392616502940655,
-0.05646122992038727,
0.013272079639136791,
0.021008362993597984,
0.002050762763246894,
0.046783458441495895,
-0.02626798488199711,
0.06903335452079773,
0.02428530342876911,
-0.012926827184855938,
-0.004614633973687887,
0.03440531715750694,
-0.011474641039967537,
-0.01120253000408411,
0.035588908940553665,
-0.007291721645742655,
0.10345139354467392,
-0.0003952303668484092,
0.03608874976634979,
-0.005984039511531591,
0.01780545897781849,
0.021331721916794777,
0.021648822352290154,
-0.0637955442070961,
-0.030563246458768845,
0.038634564727544785,
-0.01800253987312317,
0.02886863797903061,
-0.03855403512716293,
0.05282841995358467,
-0.1557108461856842,
0.06934968382120132,
0.014675723388791084,
0.05573219060897827,
-0.06524009257555008,
0.026979174464941025,
0.047034915536642075,
0.08964947611093521,
0.017636334523558617,
0.012521826662123203,
-0.008784151636064053,
0.004978339187800884,
-0.016984697431325912,
-0.024179570376873016,
0.02369885891675949,
0.01469568070024252,
-0.01111628394573927,
-0.05275445803999901,
0.04391991347074509,
0.013932380825281143,
-0.03333393484354019,
-0.021756840869784355,
0.030404284596443176,
-0.06877021491527557,
0.09869656711816788,
-0.02880668081343174,
-0.017204580828547478,
-0.00838061049580574,
-0.06855832040309906,
-0.014053740538656712,
-0.06701461225748062,
-0.032578881829977036,
-0.006271746475249529,
-0.0054894862696528435,
-0.03424021974205971,
-0.03271692618727684,
-0.06125733628869057,
0.3573092222213745,
0.016852308064699173,
0.030213046818971634,
-0.08897877484560013,
-0.00013649473839905113,
0.04913368076086044,
-0.030105045065283775,
0.025996888056397438,
-0.020304037258028984,
-0.043595075607299805,
0.048286475241184235,
0.07270755618810654,
-0.06191425770521164,
0.10179376602172852,
-0.013852912001311779,
-0.02468128688633442,
-0.0727679431438446,
0.037996575236320496,
0.009255699813365936,
0.012385732494294643,
-0.012092000804841518,
-0.023968394845724106,
-0.010607306845486164,
0.03478530794382095,
0.01517384685575962,
0.06842751801013947,
0.005164733622223139,
0.03121868520975113,
0.07353748381137848,
0.03706055134534836,
0.06312254071235657,
0.06421412527561188,
-0.005205044988542795,
-0.04176013171672821,
0.03361108899116516,
0.012703180313110352,
-0.02336106263101101,
0.07024811953306198,
0.016659457236528397,
0.03580133616924286,
-0.019761689007282257,
-0.004599348641932011,
-0.05858364701271057,
0.020787574350833893,
-0.08965451270341873,
-0.0625004991889,
0.09432025998830795,
0.05964550003409386,
-0.0044858683831989765,
-0.04223419725894928,
0.06990405172109604,
-0.06090039014816284,
0.05115339159965515,
-0.008034984581172466,
0.06722721457481384,
-0.03294598311185837,
-0.008964271284639835,
0.004712577443569899,
0.018861155956983566,
-0.02395266853272915,
-0.019266454502940178,
-0.03553013131022453,
-0.04392946884036064,
-0.028150975704193115,
0.009311482310295105,
-0.014694809913635254,
0.032928191125392914,
-0.061495330184698105,
-0.017123442143201828,
-0.036218781024217606,
0.052143827080726624,
-0.0030374322086572647,
0.02497527375817299,
0.006607046816498041,
-0.0625077337026596,
-0.0556572750210762,
0.027544191107153893,
0.033791735768318176,
-0.044018011540174484,
0.019279301166534424,
-0.036362774670124054,
0.006585822440683842,
-0.005735808052122593,
0.024838820099830627,
-0.03817157819867134,
-0.00926114246249199,
0.0361078679561615,
-0.06148744747042656,
-0.006470118183642626,
-0.05612872540950775,
0.033348824828863144,
-0.007690969854593277,
-0.03051498532295227,
-0.053664255887269974,
-0.023392094299197197,
-0.041790418326854706,
-0.03368615359067917,
0.030237993225455284,
0.00825169775635004,
-0.037314485758543015,
0.029690921306610107,
0.02946498431265354,
0.04418754577636719,
-0.021854907274246216,
0.03052208572626114,
0.01258368231356144,
0.05362675338983536,
-0.035458147525787354,
-0.0003297718067187816,
0.11159083247184753,
-0.032879382371902466,
0.016025373712182045,
0.05369116738438606,
0.07575342804193497,
0.029392404481768608,
-0.06112409010529518,
0.028287727385759354,
-0.07238798588514328,
0.03844399005174637,
0.04935469850897789,
0.002201453782618046,
0.05339433252811432,
-0.03071490302681923,
0.05960831418633461,
-0.30852454900741577,
-0.045665476471185684,
-0.032894231379032135,
-0.057624515146017075,
0.0011022526305168867,
-0.03543735295534134,
0.03173161670565605,
-0.07250815629959106,
-0.045582372695207596,
-0.04075929895043373,
0.0472569465637207,
0.06055285036563873,
0.026260100305080414,
0.011772332713007927,
-0.04207052290439606,
0.08180727809667587,
0.037114959210157394,
-0.06795817613601685,
0.01238277368247509,
-0.004593038000166416,
0.014811729080975056,
-0.03695682808756828,
-0.03161071985960007,
0.010004140436649323,
0.024243593215942383,
-0.012893520295619965,
0.08308771252632141,
0.015333457849919796,
-0.010215483605861664,
-0.024654200300574303,
-0.01705973595380783,
0.03718568757176399,
0.03288361802697182,
-0.09758570045232773,
0.035741064697504044,
0.014862015843391418,
0.042999185621738434,
0.03112764097750187,
0.049674034118652344,
0.016721056774258614,
-0.03143167123198509,
0.026248270645737648,
-0.009763226844370365,
0.006186933256685734,
-0.050356004387140274,
0.0510953851044178,
0.008967101573944092,
0.07429008185863495,
-0.06918109208345413,
0.0049603707157075405,
0.019395921379327774,
-0.0015364931896328926,
0.002024654531851411,
0.010144314728677273,
0.04512821510434151,
0.004710780922323465,
-0.08557197451591492,
-0.04001987352967262,
-0.016231000423431396,
-0.055699754506349564,
0.022911177948117256,
-0.015597897581756115,
-0.0131965521723032,
0.019646471366286278,
-0.04846471548080444,
0.04672696441411972,
0.021556079387664795,
-0.0037471014074981213,
0.049598682671785355,
0.008997287601232529,
-0.00716163357719779,
0.12599000334739685,
-0.0025412929244339466,
0.02586277201771736,
0.0380479171872139,
0.010029005818068981,
-0.033630963414907455,
-0.05870537832379341,
-0.06599544733762741,
-0.047909703105688095,
-0.007380488328635693,
-0.04171377792954445,
0.021212970837950706,
0.006233808118849993,
-0.005323267076164484,
-0.008463251404464245,
0.009179494343698025,
0.001829159096814692,
0.07259604334831238,
-0.019924869760870934,
-0.05157041922211647,
0.032598890364170074,
-0.003649060381576419,
-0.07579202204942703,
0.07428763806819916,
0.055057551711797714,
-0.2444014996290207,
-0.010583112947642803,
0.07372308522462845,
-0.032056983560323715,
0.031271934509277344,
0.0023707107175141573,
0.03535601496696472,
-0.0584845207631588,
-0.056434642523527145,
-0.02176341600716114,
-0.04236195236444473,
0.06081055477261543,
0.09733918309211731,
0.07106318324804306,
-0.05414469167590141,
-0.00449030427262187,
0.046802546828985214,
0.021722976118326187,
0.059565313160419464,
-0.016872743144631386,
0.018309349194169044,
-0.041916895657777786,
0.1429811269044876,
0.006030379328876734,
-0.0473976768553257,
0.04799625277519226,
-0.018011147156357765,
-0.005833497736603022,
-0.019300509244203568,
-0.007316575385630131,
-0.02339254505932331,
0.04522349312901497,
0.082857146859169,
-0.04244920238852501,
0.005051975604146719,
0.023048104718327522,
-0.07623995095491409,
0.006241729483008385,
0.033490799367427826,
0.014562737196683884,
-0.04798448458313942,
0.028827756643295288,
-0.06674916297197342,
-0.0184085201472044,
0.08583103865385056,
-0.0715528279542923,
-0.06541135907173157,
-0.004534101113677025,
0.016218610107898712,
0.0656258687376976,
-0.03610388934612274,
-0.020002292469143867,
0.018706070259213448,
0.03787372261285782,
0.0016090843128040433,
-0.03565302863717079,
-0.012965725734829903,
-0.0027347260620445013,
-0.015811193734407425,
-0.020131025463342667,
-0.03717713803052902,
-0.029386470094323158,
-0.00012383983994368464,
0.049823980778455734,
0.032563112676143646
] |
PMID:8320 | A kinetic analysis of a catechol-specific binding site in the microsomal fraction from the rabbit aorta. | (-)-3/-Norepinephrine (3H-NE) binding to the microsomal fraction of the rabbit aorta has been studied. Binding appears to increase linearly with time up to at least 30 min, shows no evidence of stereoselectivity and may be inhibited only by compounds possessing the catechol or 3-methoxy-4hydroxyphenyl moieties, with the latter being 100-fold less effective. 3H-NE binding is saturable with a Km of 8.5 X 10(-8) M and V max of 28 pmoles/mg protein. A Hill plot indicates that binding is noncooperative whereas a Scatchard plot suggests that two sites may be present. Binding does not appear to require physiological concentrations of Ca2+ or Mg2+ and is inhibited significantly by EDTA and sodium metabisulfite. In addition, binding is markedly enhanced by low and high pH values. This binding is also inhibited by sodium metabisulfite which suggests that an oxidized form of the catecholamine is the active binding species. Experiments with several group specific reagents indicate that binding may require a free sulfhydryl group but not a carboxyl function. The binding process requires an energy of activation of 14.8 kcal/mole whose magnitude may be partly explained, with the aid of optical rotatory dispersion spectra, by a non-stereoslective conformational change in protein structure induced by the amine. The characteristics of the 3H-NE binding sites observed in the microsomal fractional of the rabbit aorta appear to be different from those expected if binding were to the adrenoreceptors. A possible mechanism for catecholamine binding to free sulfhydryl groups on protein is presented. | A kinetic analysis of a catechol-specific binding site in the microsomal fraction from the rabbit aorta. (-)-3/-Norepinephrine (3H-NE) binding to the microsomal fraction of the rabbit aorta has been studied. Binding appears to increase linearly with time up to at least 30 min, shows no evidence of stereoselectivity and may be inhibited only by compounds possessing the catechol or 3-methoxy-4hydroxyphenyl moieties, with the latter being 100-fold less effective. 3H-NE binding is saturable with a Km of 8.5 X 10(-8) M and V max of 28 pmoles/mg protein. A Hill plot indicates that binding is noncooperative whereas a Scatchard plot suggests that two sites may be present. Binding does not appear to require physiological concentrations of Ca2+ or Mg2+ and is inhibited significantly by EDTA and sodium metabisulfite. In addition, binding is markedly enhanced by low and high pH values. This binding is also inhibited by sodium metabisulfite which suggests that an oxidized form of the catecholamine is the active binding species. Experiments with several group specific reagents indicate that binding may require a free sulfhydryl group but not a carboxyl function. The binding process requires an energy of activation of 14.8 kcal/mole whose magnitude may be partly explained, with the aid of optical rotatory dispersion spectra, by a non-stereoslective conformational change in protein structure induced by the amine. The characteristics of the 3H-NE binding sites observed in the microsomal fractional of the rabbit aorta appear to be different from those expected if binding were to the adrenoreceptors. A possible mechanism for catecholamine binding to free sulfhydryl groups on protein is presented. | [
-0.07365059107542038,
-0.01306722778826952,
-0.039156291633844376,
0.0346546433866024,
0.011008523404598236,
-0.007197520229965448,
0.058399543166160583,
-0.0026733563281595707,
-0.015822872519493103,
-0.013484960421919823,
-0.020767517387866974,
-0.06487375497817993,
-0.03402479365468025,
0.05607014149427414,
-0.022896094247698784,
-0.003758244449272752,
0.014974458143115044,
-0.010715304873883724,
-0.05458253622055054,
-0.022933777421712875,
0.011547057889401913,
0.015346559695899487,
0.009798663668334484,
0.06107429414987564,
0.06366623938083649,
0.03588935732841492,
0.029258137568831444,
0.019113369286060333,
-0.05352386087179184,
-0.196210578083992,
-0.009063433855772018,
0.008690615184605122,
0.03981638327240944,
0.007858832366764545,
-0.04482320323586464,
0.005588077940046787,
-0.017986983060836792,
-0.02451949752867222,
-0.03628680855035782,
0.054530370980501175,
0.04683609679341316,
-0.003342278301715851,
0.008389541879296303,
0.04486120119690895,
0.032574210315942764,
0.02616984210908413,
-0.04358142241835594,
-0.00549628771841526,
0.08210694789886475,
-0.013588961213827133,
-0.0045229848474264145,
0.01411518082022667,
-0.02858535386621952,
0.01856134459376335,
0.027200376614928246,
0.059936150908470154,
0.03388960659503937,
0.04080267623066902,
-0.003949889913201332,
0.03337483108043671,
-0.000619150057900697,
0.07472022622823715,
-0.1358831375837326,
0.08504588156938553,
0.0627744123339653,
0.007548093795776367,
-0.027806786820292473,
0.01880469359457493,
0.07309135794639587,
0.02671351656317711,
-0.01139136590063572,
0.017805926501750946,
-0.009954914450645447,
-0.002603641478344798,
0.013687867671251297,
0.02585608698427677,
0.026790494099259377,
-0.022731151431798935,
0.04245291277766228,
0.00019748146587517112,
0.01862199604511261,
0.019601916894316673,
-0.007804135326296091,
-0.05415863171219826,
0.012697689235210419,
-0.015206502750515938,
0.02733159065246582,
0.002786399330943823,
-0.026319874450564384,
-0.025848686695098877,
-0.037402454763650894,
-0.09683055430650711,
0.010190512984991074,
-0.021060463041067123,
-0.05346480756998062,
-0.0054826135747134686,
-0.0342438742518425,
0.008977323770523071,
-0.039696309715509415,
0.40396788716316223,
0.006096117198467255,
0.06504979729652405,
-0.06533168256282806,
0.03094320185482502,
0.049789655953645706,
-0.01046360470354557,
-0.05819718912243843,
-0.05404779687523842,
0.004200898576527834,
0.037369504570961,
0.0398547425866127,
-0.011479661799967289,
-0.004700627643615007,
-0.048657454550266266,
-0.0003999219334218651,
0.004958273842930794,
-0.03176780045032501,
-0.06180177628993988,
0.030932627618312836,
0.00348000624217093,
-0.04951482638716698,
0.01166773121803999,
-0.0035218410193920135,
0.03159071505069733,
0.08168128877878189,
-0.04603060334920883,
-0.025036955252289772,
0.08518213778734207,
0.06011727452278137,
0.03264003247022629,
0.05592496320605278,
0.02741224505007267,
-0.0684700608253479,
0.025904903188347816,
-0.020318446680903435,
-0.018488431349396706,
0.0213126540184021,
-0.007712685968726873,
-0.017126740887761116,
-0.06026136130094528,
-0.045343298465013504,
-0.07081104069948196,
-0.026161471381783485,
-0.07578437775373459,
-0.08191461116075516,
0.11764521151781082,
0.062109097838401794,
0.00568150682374835,
-0.04254293814301491,
0.014394981786608696,
0.008609454147517681,
0.035823289304971695,
-0.016437167301774025,
0.07107150554656982,
-0.048252448439598083,
0.01632806845009327,
-0.01895027421414852,
0.0057372236624360085,
-0.042535774409770966,
-0.006185444537550211,
-0.08493413031101227,
-0.011842068284749985,
0.04180995002388954,
0.0202049408107996,
-0.0038068112917244434,
-0.02633531019091606,
-0.039608076214790344,
-0.025989912450313568,
0.02288259007036686,
0.016486700624227524,
-0.0011366718681529164,
-0.009372858330607414,
0.0208014864474535,
0.01893092505633831,
-0.05537668615579605,
0.038968127220869064,
0.03984362259507179,
0.05494801327586174,
0.04666225612163544,
-0.03764604032039642,
0.023955542594194412,
0.01360737532377243,
-0.00619585532695055,
0.010759621858596802,
0.0067052519880235195,
0.004456972703337669,
-0.02244490757584572,
-0.007382986601442099,
-0.03245105966925621,
0.08447808027267456,
0.008686813525855541,
-0.0012510119704529643,
-0.10288611799478531,
-0.03425487130880356,
-0.05200925096869469,
-0.05884480103850365,
-0.0020588929764926434,
0.05946699529886246,
-0.029213419184088707,
0.032477967441082,
0.10419858247041702,
-0.03271954879164696,
-0.05168409273028374,
0.02809721603989601,
0.021166367456316948,
0.04283791407942772,
-0.0367656871676445,
0.0247596874833107,
0.050319232046604156,
-0.038943514227867126,
0.013639633543789387,
-0.016028420999646187,
-0.040106359869241714,
-0.00852728821337223,
-0.046905335038900375,
0.0103246308863163,
-0.006562704220414162,
0.024534648284316063,
0.07245107740163803,
0.0051018609665334225,
0.007894918322563171,
-0.08475832641124725,
-0.012638187035918236,
-0.2774251699447632,
-0.028012393042445183,
0.022323215380311012,
-0.015891268849372864,
0.06833026558160782,
-0.02064692974090576,
0.0324195995926857,
-0.02121630869805813,
-0.027505159378051758,
-0.08996693789958954,
0.007450418081134558,
0.01836603693664074,
-0.028347203508019447,
-0.041445184499025345,
-0.01479366235435009,
0.06586602330207825,
0.037055592983961105,
-0.04063629359006882,
0.00010160960664507002,
-0.00015892466763034463,
0.02231711708009243,
0.00720210699364543,
-0.06736302375793457,
0.028989899903535843,
0.05667034536600113,
-0.01650678738951683,
0.1056889221072197,
0.013775799423456192,
-0.06492265313863754,
-0.01242882665246725,
0.02134009823203087,
0.029016315937042236,
-0.04763149842619896,
-0.08509470522403717,
-0.04347311705350876,
-0.02725280448794365,
0.03360394015908241,
0.00221061147749424,
0.03656219318509102,
0.020198633894324303,
-0.05951966345310211,
0.030831383541226387,
0.007552952039986849,
-0.03592991083860397,
-0.07186760753393173,
-0.07076423615217209,
-0.021218465641140938,
0.0405975840985775,
0.01678290218114853,
0.014724195934832096,
0.08496822416782379,
-0.008435034193098545,
-0.03218410909175873,
-0.05195457115769386,
0.03717811778187752,
-0.0021340963430702686,
-0.008503852412104607,
0.025388501584529877,
-0.03777366504073143,
0.006701882928609848,
0.008712401613593102,
-0.025217775255441666,
0.009103194810450077,
0.027578366920351982,
0.012222263030707836,
0.006086921319365501,
0.07167685776948929,
-0.04496416449546814,
0.08873243629932404,
0.0007965920376591384,
0.031215010210871696,
0.094728983938694,
-0.010988838039338589,
0.0631769448518753,
0.06525557488203049,
0.004588460549712181,
-0.036379940807819366,
-0.01347372867166996,
0.000736396003048867,
0.06303634494543076,
0.009137189015746117,
-0.05925518646836281,
0.05370836704969406,
0.0294719859957695,
-0.03580198436975479,
-0.011186903342604637,
-0.006453967187553644,
-0.01918608322739601,
0.0653240904211998,
-0.004013439640402794,
0.002188894199207425,
0.02199219912290573,
-0.017459582537412643,
-0.02947518415749073,
0.06445342302322388,
0.0070546576753258705,
-0.26570212841033936,
0.010247942060232162,
0.01070595532655716,
0.0020427792333066463,
-0.0284871906042099,
0.012614287436008453,
0.027894901111721992,
-0.054560884833335876,
-0.07101520150899887,
0.08521240204572678,
-0.022045770660042763,
0.031231718137860298,
0.05175445228815079,
0.03402605652809143,
-0.0066009401343762875,
0.007149721495807171,
0.0396110899746418,
-0.03383990004658699,
-0.023335764184594154,
-0.031240662559866905,
-0.025557398796081543,
-0.01108909584581852,
0.141371488571167,
0.0013138387585058808,
-0.04526019096374512,
0.018176080659031868,
-0.06053348258137703,
-0.008469455875456333,
-0.08840426802635193,
0.005147040355950594,
-0.022599855437874794,
-0.0016978755593299866,
0.0686834380030632,
-0.05385211110115051,
0.028970051556825638,
0.039461392909288406,
-0.05997377261519432,
-0.028028490021824837,
-0.0010053601581603289,
0.035708654671907425,
-0.007982819341123104,
0.04644284024834633,
-0.06559848040342331,
-0.022270409390330315,
0.064450204372406,
-0.04370018094778061,
-0.06203118711709976,
0.026838907971978188,
0.03699667379260063,
0.004362679552286863,
-0.023991968482732773,
0.03838836029171944,
-0.015927938744425774,
0.05548841506242752,
0.005089527927339077,
0.009016213938593864,
-0.026039307937026024,
-0.009232263080775738,
-0.01729964278638363,
-0.006337688770145178,
-0.05692470446228981,
0.026516888290643692,
-0.04251456633210182,
0.10017140209674835,
0.06533738225698471
] |
PMID:8321 | Alpha-adrenergic activity of N,N-dimethyldopamine (DMDA). | The autonomic and cardiovascular activity of N,N-dimethyldopamine (DMDA) was studied in the dog and rabbit. DMDA appears to be a postganglionic sympathetic alpha-adrenoceptor agonist since it consistently caused vasconstriction in several isolated vascular beds, even in the presence of ganglionic blockade. The pressor activity of DMDA was attenuated by the alpha-adrenergic antagonist phentolamine. Calculated pA2 values for phentolamine against both DMDA and norepinephrine in isolated rabbit arteries were in close agreement. DMDA inhibits cardioaccelerator nerve stimulation induced chronotropic responses by a mechanism which appears to be alpha-adrenergic in the dog. | Alpha-adrenergic activity of N,N-dimethyldopamine (DMDA). The autonomic and cardiovascular activity of N,N-dimethyldopamine (DMDA) was studied in the dog and rabbit. DMDA appears to be a postganglionic sympathetic alpha-adrenoceptor agonist since it consistently caused vasconstriction in several isolated vascular beds, even in the presence of ganglionic blockade. The pressor activity of DMDA was attenuated by the alpha-adrenergic antagonist phentolamine. Calculated pA2 values for phentolamine against both DMDA and norepinephrine in isolated rabbit arteries were in close agreement. DMDA inhibits cardioaccelerator nerve stimulation induced chronotropic responses by a mechanism which appears to be alpha-adrenergic in the dog. | [
-0.05098909139633179,
-0.02402353100478649,
-0.024971021339297295,
0.00901009701192379,
-0.021171679720282555,
-0.004516942892223597,
0.03339346870779991,
0.005613501183688641,
-0.005413437727838755,
-0.08126211166381836,
0.03134520724415779,
-0.04298381134867668,
0.004837673623114824,
0.019735395908355713,
-0.025968514382839203,
-0.025866413488984108,
0.014709306880831718,
0.0010648121824488044,
-0.025746069848537445,
0.022247787564992905,
-0.003429287113249302,
0.03274830803275108,
0.016975879669189453,
-0.019198184832930565,
0.0479290708899498,
0.05892915651202202,
-0.019288836047053337,
-0.0034897385630756617,
-0.020519427955150604,
-0.15074926614761353,
0.03577372804284096,
-0.020241646096110344,
0.034821584820747375,
-0.05775710940361023,
-0.04660661891102791,
-0.000764401163905859,
-0.025391507893800735,
0.026267679408192635,
-0.0184932891279459,
0.031149519607424736,
0.08603209257125854,
-0.06638951599597931,
0.005176250822842121,
0.011758370324969292,
0.029097560793161392,
0.017060933634638786,
-0.0533052496612072,
0.002865031361579895,
0.0927068218588829,
0.030103296041488647,
0.06551636010408401,
-0.0035317239817231894,
0.020051032304763794,
0.003944729920476675,
0.055086974054574966,
-0.026961393654346466,
0.0017990689957514405,
0.028563855215907097,
0.013419603928923607,
0.028954433277249336,
0.06577503681182861,
0.06942532956600189,
-0.16802997887134552,
0.05289887636899948,
0.07245540618896484,
0.012495923787355423,
-0.005981639493256807,
-0.011366880498826504,
0.026387466117739677,
0.04997922480106354,
-0.006371954455971718,
0.04727047681808472,
0.027901774272322655,
-0.04658351466059685,
0.007481004111468792,
-0.0034475326538085938,
0.01612427458167076,
-0.0146159827709198,
0.052465010434389114,
-0.004762641619890928,
0.012508966960012913,
-0.0014235805720090866,
0.024910524487495422,
-0.032842520624399185,
0.04782232642173767,
0.00849116314202547,
0.019327333196997643,
-0.007232438772916794,
-0.05095944181084633,
-0.022085733711719513,
-0.04523945227265358,
-0.016750359907746315,
0.01752353087067604,
0.0041253347881138325,
-0.04434127360582352,
0.021623337641358376,
0.044837307184934616,
0.005081251263618469,
-0.07956718653440475,
0.3230471909046173,
0.012362646870315075,
0.047858159989118576,
-0.10952768474817276,
-0.034835778176784515,
0.036954160779714584,
-0.0077414256520569324,
0.010115223005414009,
-0.020803308114409447,
-0.03599740192294121,
0.09248881042003632,
0.12309174984693527,
0.066210076212883,
0.020688146352767944,
-0.08643852919340134,
-0.021041953936219215,
-0.09276088327169418,
0.04099179431796074,
0.04475130885839462,
0.028851618990302086,
0.029325878247618675,
0.005589064676314592,
-0.024086106568574905,
-0.009690893813967705,
0.014439254999160767,
0.02007499523460865,
0.020754756405949593,
-0.055842820554971695,
0.11496217548847198,
0.019675621762871742,
-0.010217676870524883,
0.03137940540909767,
-0.0016096087638288736,
0.006048722192645073,
0.01898837275803089,
0.08295250684022903,
-0.018957944586873055,
0.08977746963500977,
-0.03672366216778755,
-0.008777905255556107,
-0.017785053700208664,
-0.04062092304229736,
-0.02605925314128399,
-0.03387761488556862,
-0.05149766430258751,
-0.06359296292066574,
0.10608487576246262,
0.04995855316519737,
0.013187031261622906,
-0.047342054545879364,
0.05870097130537033,
0.0049788085743784904,
0.06026466563344002,
-0.01852203719317913,
0.08718851208686829,
-0.0278287585824728,
0.04138055071234703,
-0.001602261676453054,
0.005441255867481232,
0.023255780339241028,
-0.02898103930056095,
-0.07389865815639496,
-0.061791591346263885,
0.0036428896710276604,
0.05539429932832718,
0.014776156283915043,
-0.029161008074879646,
-0.01568114571273327,
0.028720935806632042,
-0.009214760735630989,
0.00031644507544115186,
-0.012044020928442478,
0.04560155048966408,
-0.06123917177319527,
-0.05395713821053505,
-0.05888985097408295,
-0.007659876253455877,
0.03797079995274544,
-0.024115830659866333,
0.03248319774866104,
0.02465873211622238,
0.04753143712878227,
-0.031135009601712227,
0.032428525388240814,
-0.012632029131054878,
-0.0016062604263424873,
0.064745232462883,
-0.03588733822107315,
-0.02557782270014286,
0.02638908103108406,
-0.039198145270347595,
0.015793083235621452,
-0.039265476167201996,
-0.06370784342288971,
0.011868821457028389,
-0.0695597380399704,
-0.032628919929265976,
-0.03956975042819977,
0.06122271716594696,
-0.02961825579404831,
0.0006053490797057748,
0.025366727262735367,
0.051630254834890366,
-0.007311886642128229,
0.02910454198718071,
0.02441013976931572,
0.048041678965091705,
-0.0651719942688942,
-0.0346391536295414,
0.029398944228887558,
-0.00982972513884306,
0.008533900603652,
0.024689270183444023,
0.04129274562001228,
-0.02384175918996334,
-0.06267722696065903,
0.07759623229503632,
-0.05658910050988197,
0.03403102979063988,
0.07362077385187149,
-0.048230379819869995,
0.03560078144073486,
-0.024624763056635857,
0.005116445943713188,
-0.31609535217285156,
-0.03740392625331879,
-0.020046858116984367,
-0.02541971765458584,
-0.03949751704931259,
-0.0745832696557045,
0.06592283397912979,
-0.06414245069026947,
0.00363529403693974,
-0.08390742540359497,
-0.0005532291252166033,
0.04897354170680046,
-0.020606521517038345,
-0.05032527446746826,
0.017306474968791008,
0.11597134172916412,
0.03162647783756256,
-0.021605949848890305,
-0.0039022481068968773,
0.01814318262040615,
0.03150125592947006,
0.026588721200823784,
-0.03417811915278435,
0.022146685048937798,
0.03586244210600853,
-0.0075577483512461185,
0.1097642332315445,
0.045180246233940125,
-0.044319234788417816,
-0.008500788360834122,
0.009211555123329163,
0.09101913124322891,
-0.08105497062206268,
-0.09281308948993683,
-0.007403118535876274,
-0.06717534363269806,
0.030920138582587242,
0.0022103835362941027,
0.022263994440436363,
-0.004660736303776503,
-0.03034389577805996,
0.06506999582052231,
0.0000038978578231763095,
-0.013490891084074974,
-0.020122069865465164,
-0.04167851060628891,
0.0053702546283602715,
0.05359432473778725,
-0.019248928874731064,
-0.047884829342365265,
0.03874829038977623,
-0.05350467935204506,
-0.043483782559633255,
-0.01023617573082447,
-0.041660428047180176,
-0.025140391662716866,
-0.0685235932469368,
-0.03859827294945717,
-0.038935448974370956,
-0.023873548954725266,
0.00937693566083908,
0.03520127385854721,
0.05015711858868599,
-0.02618553303182125,
-0.039844222366809845,
0.03043225407600403,
0.03105289116501808,
0.03429805114865303,
0.08377975970506668,
0.008319336920976639,
0.01516671571880579,
0.08181736618280411,
0.013653713278472424,
0.04509325698018074,
0.09557631611824036,
-0.0017194299725815654,
-0.05058128759264946,
0.0030560391023755074,
-0.07996807992458344,
0.02402142994105816,
-0.017859065905213356,
-0.05538421496748924,
0.020766286179423332,
0.052036501467227936,
-0.03490760549902916,
-0.01746070384979248,
0.035901229828596115,
0.053934890776872635,
0.00910499319434166,
0.01293268147855997,
-0.0028685489669442177,
0.02187737077474594,
-0.030956842005252838,
-0.06961824744939804,
0.004818023182451725,
0.05690972879528999,
-0.2486678808927536,
-0.04947122186422348,
0.040445152670145035,
-0.02741312049329281,
-0.028843024745583534,
0.008673468604683876,
-0.0272830780595541,
-0.027445392683148384,
-0.03351452574133873,
0.02257014997303486,
-0.0407218374311924,
0.058962270617485046,
0.002585698151960969,
0.02960919961333275,
-0.0817914605140686,
0.018425071612000465,
0.045388925820589066,
0.04773619398474693,
0.004413117188960314,
-0.07581523060798645,
0.00205774768255651,
-0.0024466628674417734,
0.11472777277231216,
-0.03041457198560238,
-0.04946538805961609,
0.05056222900748253,
-0.060047343373298645,
0.039149265736341476,
-0.026845358312129974,
-0.025686364620923996,
-0.0260413009673357,
-0.017550919204950333,
0.1120789423584938,
-0.03726866468787193,
0.03293006867170334,
0.022930383682250977,
-0.07842763513326645,
0.033606093376874924,
0.009908719919621944,
-0.02479989267885685,
-0.03701872006058693,
0.031661152839660645,
-0.020114554092288017,
-0.03741256520152092,
0.06747071444988251,
-0.049834322184324265,
-0.05534215644001961,
-0.01529364101588726,
-0.0008441743557341397,
0.02286163717508316,
0.040487442165613174,
-0.0313832126557827,
0.019276313483715057,
0.0042081899009644985,
0.01365890447050333,
0.012091586366295815,
-0.02523144893348217,
-0.019030217081308365,
0.060366831719875336,
-0.07173318415880203,
-0.017116913571953773,
-0.011354906484484673,
-0.017991958186030388,
0.033499978482723236,
0.028219928964972496
] |
PMID:8327 | Glycoprotein biosynthesis in splenic cells. Purification of a microsomal galactosyl-transferase. | One part of the microsomal galactosyl-transferase activity of splenic cells of rats can by solubilized by the action of Triton X-100 and Tween 20. Its purification on a Sephadex G-200 column and by electrophoresis on a polyacrylamide gel leads to a solution of high specific enzymic activity. | Glycoprotein biosynthesis in splenic cells. Purification of a microsomal galactosyl-transferase. One part of the microsomal galactosyl-transferase activity of splenic cells of rats can by solubilized by the action of Triton X-100 and Tween 20. Its purification on a Sephadex G-200 column and by electrophoresis on a polyacrylamide gel leads to a solution of high specific enzymic activity. | [
-0.008987922221422195,
-0.02957134135067463,
-0.016651833429932594,
-0.03596603497862816,
-0.005199137609452009,
-0.09141618013381958,
0.016828598454594612,
0.039356570690870285,
-0.0677843987941742,
-0.011182655580341816,
0.014818140305578709,
-0.0935640037059784,
-0.011821446008980274,
-0.02501622773706913,
-0.06867661327123642,
-0.04086955636739731,
0.018808554857969284,
0.015402560122311115,
-0.10144214332103729,
-0.006927726324647665,
-0.04124777764081955,
-0.013975819572806358,
0.0027358478400856256,
0.05046302452683449,
0.030548037961125374,
-0.002912161173298955,
0.0383748784661293,
-0.039427850395441055,
-0.05057244375348091,
-0.1538999080657959,
-0.001273160451091826,
-0.053952690213918686,
0.04509400576353073,
-0.011336962692439556,
0.013094233348965645,
-0.02151438221335411,
-0.037769146263599396,
-0.018567882478237152,
-0.013635537587106228,
0.10521398484706879,
0.055561549961566925,
0.008532584644854069,
-0.016479825600981712,
0.019580701366066933,
0.037408582866191864,
0.032080236822366714,
-0.030762679874897003,
-0.008016553707420826,
0.06479677557945251,
0.002166744787245989,
-0.03779621049761772,
0.03219128027558327,
0.011779965832829475,
0.02817254513502121,
-0.013712191954255104,
0.002323645167052746,
0.028409183025360107,
0.08262283354997635,
0.016125084832310677,
0.05187804624438286,
-0.03001505322754383,
0.031497836112976074,
-0.09899846464395523,
0.08428959548473358,
0.035889703780412674,
0.028944944962859154,
0.02337830513715744,
-0.05293499678373337,
0.03472697734832764,
0.019995030015707016,
-0.08188702166080475,
-0.0024982502218335867,
-0.024877851828932762,
0.048738494515419006,
-0.010364960879087448,
-0.012286397628486156,
0.02434164471924305,
0.020776158198714256,
0.047361310571432114,
0.08132559061050415,
0.0003546701336745173,
0.002404632279649377,
0.05212024226784706,
0.004073931369930506,
-0.051340408623218536,
0.04659609496593475,
-0.02709115669131279,
-0.008264685980975628,
0.04489210620522499,
0.05857463926076889,
-0.027841657400131226,
-0.04999566078186035,
0.010963442735373974,
0.015281589701771736,
-0.049017906188964844,
0.028829680755734444,
0.01058702077716589,
0.006706359796226025,
0.024591587483882904,
0.3730708956718445,
0.024661747738718987,
0.043931473046541214,
-0.04910030961036682,
0.012151578441262245,
-0.011590411886572838,
-0.03649787977337837,
-0.05689432844519615,
0.0019610074814409018,
-0.048029132187366486,
0.03874441608786583,
0.0684410035610199,
0.03853535279631615,
0.04212670028209686,
-0.01572250761091709,
-0.002967956941574812,
-0.0019272677600383759,
0.017046945169568062,
-0.00105752341914922,
0.010891392827033997,
0.04886120930314064,
-0.015271345153450966,
-0.02615617960691452,
-0.002236113417893648,
-0.015857558697462082,
0.06904295086860657,
0.05595212057232857,
-0.02386678196489811,
0.0878574326634407,
0.07440248876810074,
0.03988013043999672,
0.02342015504837036,
0.09811016172170639,
-0.03955816105008125,
-0.014211457222700119,
0.012207151390612125,
-0.06136114150285721,
0.02323930338025093,
-0.058593470603227615,
-0.06485814601182938,
-0.043508533388376236,
-0.0004265732422936708,
-0.009949641302227974,
0.005567549262195826,
-0.05753542110323906,
-0.019606968387961388,
0.07304925471544266,
0.0068540857173502445,
0.0072170705534517765,
0.018749848008155823,
0.02665484882891178,
-0.010914644226431847,
0.056319400668144226,
-0.002931119641289115,
0.03698324039578438,
0.016010252758860588,
0.0347689725458622,
0.05163060128688812,
-0.04026846960186958,
0.01492338441312313,
-0.005414982326328754,
-0.03662113845348358,
0.025892702862620354,
0.016574371606111526,
0.0958060473203659,
0.06989000737667084,
0.03445893153548241,
-0.022421512752771378,
0.014837789349257946,
-0.0003537389275152236,
-0.059165313839912415,
-0.010593759827315807,
0.003106856718659401,
-0.053176820278167725,
-0.040800031274557114,
-0.033152300864458084,
0.031658004969358444,
-0.0036284748930484056,
-0.03157486394047737,
0.021902361884713173,
0.009073358029127121,
0.04678129404783249,
-0.03630157187581062,
-0.040619321167469025,
0.02501857466995716,
0.025914395228028297,
-0.026317138224840164,
0.010014829225838184,
0.02157255820930004,
0.007305006496608257,
0.1107720360159874,
-0.02787654846906662,
0.06415211409330368,
-0.03877657279372215,
-0.034229930490255356,
-0.012840543873608112,
-0.03993178531527519,
-0.006416009739041328,
-0.008105060085654259,
0.030401835218071938,
-0.01913652941584587,
0.08551060408353806,
-0.008848757483065128,
-0.017806343734264374,
0.07103516906499863,
-0.03261803463101387,
0.025888560339808464,
0.08397962898015976,
0.002015874022617936,
0.05072620138525963,
0.019623422995209694,
-0.026549922302365303,
0.011476953513920307,
-0.024434614926576614,
0.04828919842839241,
-0.05967770889401436,
0.04247395321726799,
-0.018939444795250893,
-0.013881430961191654,
0.06811542809009552,
-0.020994527265429497,
-0.0017637218115851283,
-0.07657133787870407,
-0.03609476238489151,
-0.31039324402809143,
-0.00643555074930191,
-0.005844080820679665,
-0.07178863883018494,
0.00419025681912899,
-0.07256467640399933,
-0.012105748988687992,
-0.049821991473436356,
-0.01586952991783619,
-0.022910740226507187,
0.09083890914916992,
0.07614248245954514,
-0.04585487022995949,
-0.0414893701672554,
-0.029425326734781265,
0.03670668229460716,
-0.04129230976104736,
0.0025280937552452087,
0.06527552753686905,
-0.0046319602988660336,
0.044756416231393814,
-0.017919516190886497,
0.04457283020019531,
-0.0005761579959653318,
0.009243874810636044,
-0.06181368976831436,
0.0920129269361496,
0.04324683919548988,
-0.03474164009094238,
-0.019226722419261932,
0.03077247552573681,
0.022992193698883057,
-0.01979963108897209,
-0.08189567178487778,
-0.02326919324696064,
-0.04208027943968773,
-0.04029756784439087,
-0.055641718208789825,
0.051697514951229095,
0.007308764383196831,
-0.025949005037546158,
0.011848979629576206,
-0.02746286801993847,
-0.03605682775378227,
0.007678063120692968,
0.002270553959533572,
0.0028984728269279003,
0.0364835299551487,
-0.011364460922777653,
0.010324002243578434,
0.031927816569805145,
0.013855567201972008,
0.03352929279208183,
-0.011598999612033367,
0.013903015293180943,
0.013987303711473942,
-0.02114950306713581,
0.018163472414016724,
-0.050323646515607834,
0.028954781591892242,
0.0014983715955168009,
0.0007167132571339607,
-0.042938847094774246,
-0.001611243700608611,
0.020380103960633278,
0.06450893729925156,
-0.005969534162431955,
-0.06754489243030548,
0.032423269003629684,
-0.005930393468588591,
-0.01410177256911993,
0.08066465705633163,
-0.013548183254897594,
0.07899617403745651,
-0.01072897482663393,
0.03276209533214569,
0.020878199487924576,
-0.0133842583745718,
-0.12897057831287384,
0.030796494334936142,
0.006352015305310488,
-0.03505467250943184,
0.04884585738182068,
0.022731373086571693,
-0.0042296224273741245,
0.028894538059830666,
0.006909811869263649,
0.014178561978042126,
-0.02524556592106819,
-0.028637908399105072,
-0.025342965498566628,
-0.012759393081068993,
-0.04155964031815529,
-0.009819164872169495,
0.045418042689561844,
-0.03827708214521408,
-0.2811439633369446,
-0.004944930784404278,
-0.003931134007871151,
-0.04052204266190529,
-0.01753232814371586,
-0.002952963812276721,
0.06518169492483139,
-0.022011954337358475,
-0.037454694509506226,
0.052650511264801025,
0.05048012360930443,
-0.00635721767321229,
0.10030631721019745,
-0.020750170573592186,
0.007673708721995354,
-0.00957936979830265,
0.05649935454130173,
-0.04109582304954529,
0.0021508848294615746,
-0.008137006312608719,
0.0217119287699461,
-0.042419858276844025,
0.15065941214561462,
0.007489823270589113,
0.01364931184798479,
0.07860645651817322,
-0.05010044574737549,
-0.004315508063882589,
-0.006473790388554335,
0.042707670480012894,
0.06512165814638138,
0.01550065353512764,
0.04249037429690361,
-0.099533312022686,
-0.02109401673078537,
0.035537514835596085,
-0.042384762316942215,
-0.03805034980177879,
0.003803638508543372,
0.015122845768928528,
-0.0035966825671494007,
-0.0036068353801965714,
-0.04654618725180626,
-0.0790543481707573,
0.07033394277095795,
-0.09735511243343353,
-0.0449691005051136,
-0.06257786601781845,
-0.041343264281749725,
0.004029455594718456,
-0.09503281116485596,
-0.0329391285777092,
0.02255985327064991,
-0.040849510580301285,
0.008950009942054749,
0.017979761585593224,
-0.020340612158179283,
0.03287371248006821,
-0.0359702929854393,
0.0006596670718863606,
-0.025865137577056885,
-0.051051683723926544,
-0.01282196119427681,
0.08537221699953079,
0.03128645569086075
] |
PMID:8329 | Beta-adrenergic blocking agents as potent antagonists of mescaline-induced contractions in the rat uterus. | In the isolated rat uterus, mescaline induces contractions that are notably antagonized by catecholamines, by beta-adrenergic stimulants and certain beta-adrenergic blocking agents as well as by chlorpromazine, amitriptyline and methysergide. | Beta-adrenergic blocking agents as potent antagonists of mescaline-induced contractions in the rat uterus. In the isolated rat uterus, mescaline induces contractions that are notably antagonized by catecholamines, by beta-adrenergic stimulants and certain beta-adrenergic blocking agents as well as by chlorpromazine, amitriptyline and methysergide. | [
-0.045605193823575974,
-0.021009400486946106,
-0.005159182474017143,
0.05467309430241585,
-0.00021798927627969533,
0.003477010875940323,
0.011213419027626514,
0.03231455013155937,
-0.03249650448560715,
-0.019367096945643425,
-0.017396584153175354,
-0.01799692213535309,
-0.03258654475212097,
0.08125282824039459,
-0.01168094389140606,
-0.015317688696086407,
0.06435326486825943,
0.03963731601834297,
-0.07115741074085236,
-0.01239854283630848,
-0.011136495508253574,
0.035207923501729965,
-0.03203460946679115,
0.018134111538529396,
0.024047547951340675,
0.02010027877986431,
-0.031416893005371094,
0.013847162015736103,
-0.05792330577969551,
-0.14024129509925842,
-0.006772293243557215,
0.017151394858956337,
0.024488400667905807,
-0.02419264428317547,
0.040103428065776825,
0.0003859616699628532,
-0.009821918793022633,
0.03223613277077675,
-0.00945792905986309,
0.06477371603250504,
0.02543088048696518,
-0.01141887716948986,
-0.015052911825478077,
0.0053018913604319096,
0.016362739726901054,
0.0023140853736549616,
0.039813827723264694,
-0.0454116091132164,
0.0752573013305664,
0.00977677945047617,
0.058809977024793625,
0.02762925811111927,
0.022074861451983452,
0.012629939243197441,
0.020061872899532318,
-0.04032332822680473,
-0.03560218587517738,
0.04976195469498634,
0.04302414134144783,
0.03405012562870979,
-0.00920514203608036,
0.025625113397836685,
-0.17811928689479828,
0.05114699900150299,
0.013132788240909576,
0.0370447151362896,
0.024462400004267693,
0.019934721291065216,
0.017859993502497673,
0.10246281325817108,
0.0008405144908465445,
0.06743768602609634,
-0.04130886122584343,
-0.01165700238198042,
0.007487277965992689,
-0.0060538118705153465,
-0.0015701467636972666,
-0.02550995536148548,
0.0031478104647248983,
0.0037148436531424522,
0.044826727360486984,
-0.05128435045480728,
-0.007888481952250004,
-0.06025376170873642,
0.038977090269327164,
-0.036711033433675766,
0.06530134379863739,
0.012809215113520622,
0.004586380906403065,
-0.07153350114822388,
-0.05437092483043671,
-0.07123085111379623,
0.0011872542090713978,
-0.02589293383061886,
-0.04490751028060913,
-0.018820082768797874,
-0.060958076268434525,
-0.016902321949601173,
-0.04048027843236923,
0.32666051387786865,
-0.03064095601439476,
0.036027416586875916,
-0.04731069505214691,
0.03304449841380119,
-0.007022422272711992,
0.05054835230112076,
-0.025917818769812584,
-0.0263096634298563,
-0.04699688404798508,
0.07962335646152496,
0.05045003443956375,
-0.0012776845833286643,
-0.003038759110495448,
-0.058897290378808975,
-0.035850297659635544,
-0.12267748266458511,
0.0327434279024601,
-0.007792653515934944,
0.0219520665705204,
0.02091977745294571,
-0.05963118374347687,
-0.04612497240304947,
0.031200172379612923,
-0.00406205840408802,
0.044853877276182175,
-0.015906278043985367,
-0.030102653428912163,
0.09819664806127548,
0.05600690469145775,
-0.017371978610754013,
0.04594177380204201,
0.021801600232720375,
-0.043520454317331314,
0.06888878345489502,
0.026337748393416405,
-0.022861285135149956,
-0.00357623933814466,
-0.01249298732727766,
-0.0004884214722551405,
-0.0783541202545166,
-0.04027286171913147,
-0.07563713192939758,
0.02905901148915291,
-0.104510098695755,
-0.04119950532913208,
0.08385774493217468,
0.03717074170708656,
0.00906371884047985,
-0.060107383877038956,
0.036102332174777985,
-0.030527736991643906,
0.046907924115657806,
-0.02252170816063881,
0.07017561793327332,
-0.06938079744577408,
0.0403614304959774,
0.02211099863052368,
-0.003966954071074724,
-0.041541073471307755,
-0.04513826221227646,
-0.06758447736501694,
-0.0197463296353817,
-0.00844624638557434,
0.04185608774423599,
0.030830126255750656,
-0.03513360396027565,
-0.03825303912162781,
0.027490951120853424,
-0.02080661803483963,
0.0016489289700984955,
-0.0448743999004364,
-0.029699286445975304,
-0.014292147941887379,
-0.05788930878043175,
-0.05682436376810074,
-0.022782588377594948,
0.0443185418844223,
-0.009850402362644672,
0.046615179628133774,
-0.03381035476922989,
0.03194286301732063,
-0.0035376527812331915,
0.045340076088905334,
0.009658717550337315,
0.05256948992609978,
0.0012406788300722837,
-0.028251370415091515,
-0.029804231598973274,
-0.004462111741304398,
-0.0071906475350260735,
-0.04588860645890236,
-0.014924920164048672,
-0.07792972028255463,
0.01694977469742298,
-0.0607670433819294,
-0.02144692838191986,
0.001267704414203763,
0.047651998698711395,
-0.05295620858669281,
-0.016313571482896805,
0.04357590898871422,
0.006489507853984833,
-0.016543807461857796,
0.10402517765760422,
0.03839275240898132,
0.053603801876306534,
-0.011781549081206322,
0.003887348109856248,
0.04468519613146782,
-0.02207033336162567,
0.006099172867834568,
0.09235695004463196,
0.08318577706813812,
-0.023845549672842026,
-0.07493255287408829,
0.05442793294787407,
-0.010914238169789314,
0.005367932841181755,
0.09043331444263458,
0.024147439748048782,
0.0502193458378315,
0.04068995267152786,
-0.005437957122921944,
-0.2967773675918579,
-0.03962484747171402,
-0.01406670268625021,
-0.0651615709066391,
0.07414824515581131,
-0.02633076161146164,
0.02978435717523098,
-0.06281816959381104,
-0.031060578301548958,
-0.05305515229701996,
0.07995393127202988,
0.04023955017328262,
-0.02728702500462532,
0.0011592580704018474,
0.012329939752817154,
0.02741995081305504,
0.007776427548378706,
-0.05826490372419357,
0.03173818066716194,
0.049597710371017456,
0.023004725575447083,
-0.02145048789680004,
-0.048907287418842316,
0.04147944226861,
0.031398095190525055,
0.03098774515092373,
0.10190510004758835,
0.0441342368721962,
-0.03990067541599274,
-0.003642447292804718,
0.014183521270751953,
0.03979799896478653,
-0.0163103137165308,
-0.0878080353140831,
-0.014672672376036644,
-0.04278706759214401,
0.08548376709222794,
-0.031075673177838326,
0.03665243089199066,
-0.015154342167079449,
-0.08636127412319183,
0.0009401196730323136,
0.015554683282971382,
-0.0014047930017113686,
-0.04714908450841904,
0.001641295151785016,
-0.016223320737481117,
0.05132485181093216,
0.007546841166913509,
-0.06032584235072136,
-0.005054726731032133,
0.041411686688661575,
0.0019084974192082882,
-0.0524623729288578,
0.024758489802479744,
0.0453721359372139,
-0.11540837585926056,
0.004771334119141102,
-0.0034660599194467068,
-0.04748417064547539,
0.030976317822933197,
-0.018927764147520065,
0.045590195804834366,
-0.02596101351082325,
-0.013822157867252827,
0.007066550198942423,
0.038286495953798294,
0.011484683491289616,
0.00707806833088398,
0.012872596271336079,
0.031623128801584244,
0.12274888902902603,
-0.05102067440748215,
0.014263756573200226,
0.005971560254693031,
0.0159787405282259,
-0.011029877699911594,
-0.004412700887769461,
-0.08437688648700714,
-0.0028677142690867186,
-0.02367350645363331,
-0.020176243036985397,
0.06797431409358978,
0.028369037434458733,
-0.026181811466813087,
-0.01927199959754944,
0.0009557989542372525,
0.024774176999926567,
0.13690653443336487,
0.026432758197188377,
-0.015480678528547287,
0.061342958360910416,
-0.04772641509771347,
-0.012148772366344929,
0.06508718430995941,
-0.02122066542506218,
-0.2724097669124603,
0.02544540911912918,
0.04063932225108147,
-0.035139113664627075,
-0.02272658981382847,
0.029225608333945274,
0.04275841265916824,
-0.03638504818081856,
-0.08500445634126663,
-0.019577756524086,
-0.01762085221707821,
0.03004351258277893,
0.04952389374375343,
0.029352910816669464,
-0.02739008143544197,
0.040434226393699646,
0.04909021779894829,
0.042633671313524246,
0.046282198280096054,
-0.03944803774356842,
-0.011044207029044628,
-0.012011478655040264,
0.1515069454908371,
-0.021464010700583458,
-0.002135742688551545,
0.0426812618970871,
0.010943427681922913,
0.018693968653678894,
0.020385561510920525,
0.04809172824025154,
0.003033261513337493,
0.0010507889091968536,
0.054584477096796036,
-0.050437599420547485,
0.008319932036101818,
-0.0030794625636190176,
-0.07076512277126312,
0.01169500406831503,
0.05432334914803505,
-0.010752570815384388,
-0.07193026691675186,
0.06986214220523834,
-0.06699121743440628,
-0.0034979877527803183,
0.02999219112098217,
-0.07184422016143799,
-0.0633917823433876,
-0.01735992170870304,
0.008770356886088848,
0.037680353969335556,
0.03256876394152641,
-0.005230914335697889,
0.03386968746781349,
0.050543177872896194,
0.0011135537642985582,
-0.024235375225543976,
-0.033778078854084015,
-0.011591927148401737,
0.0047047073021531105,
0.02591267041862011,
-0.046234894543886185,
-0.03123369812965393,
-0.03926042467355728,
0.021368324756622314,
0.020010443404316902
] |
PMID:8330 | Influence of castration and testosterone replacement on hypothalamic tyrosine hydroxylase activity in the rat. | Hypothalamic tyrosine hydroxylase (TH) activity of castrate rats is modulated by testosterone propionate (TP) in vivo. Kinetic studies revealed that both Vmax and Km were virtually unaltered for substrate tyrosine in the presence of an excess of DMPH4 cofactor. TP replacement to castrate rats increased the Km for added DMPH4 cofactor, while Vmax decreased. These results suggest that TP decreases TH activity of castrate rats by inhibiting the enzyme-reduced pteridine cofactor complex. | Influence of castration and testosterone replacement on hypothalamic tyrosine hydroxylase activity in the rat. Hypothalamic tyrosine hydroxylase (TH) activity of castrate rats is modulated by testosterone propionate (TP) in vivo. Kinetic studies revealed that both Vmax and Km were virtually unaltered for substrate tyrosine in the presence of an excess of DMPH4 cofactor. TP replacement to castrate rats increased the Km for added DMPH4 cofactor, while Vmax decreased. These results suggest that TP decreases TH activity of castrate rats by inhibiting the enzyme-reduced pteridine cofactor complex. | [
-0.023557104170322418,
0.03294017165899277,
-0.0010508907726034522,
0.006210405379533768,
-0.037587739527225494,
0.020659994333982468,
0.0801728367805481,
0.01459374651312828,
-0.04796501621603966,
-0.06351273506879807,
0.024342812597751617,
-0.05128196254372597,
0.052345335483551025,
0.040687885135412216,
-0.02754851244390011,
-0.0025341082364320755,
0.020211203023791313,
-0.008066978305578232,
-0.05122574791312218,
-0.0026856998447328806,
0.0019600563682615757,
0.018456557765603065,
0.04355514422059059,
0.004189721774309874,
0.04061517491936684,
-0.020631905645132065,
-0.042167916893959045,
0.020258953794836998,
0.0035797106102108955,
-0.18251004815101624,
0.06101949140429497,
-0.058371514081954956,
-0.035805463790893555,
-0.036551158875226974,
-0.025851106271147728,
0.0066944570280611515,
-0.027700942009687424,
0.0014134321827441454,
-0.006036678329110146,
0.034144822508096695,
0.028451604768633842,
0.036322396248579025,
-0.04187938943505287,
-0.04179440438747406,
-0.02089511603116989,
0.03647865355014801,
-0.06007581204175949,
-0.026728656142950058,
0.06036883220076561,
0.00255706743337214,
-0.005379370413720608,
0.010853839106857777,
0.05057068541646004,
0.09765631705522537,
0.02051835134625435,
0.035078804939985275,
0.056568246334791183,
0.05325113236904144,
-0.00500883162021637,
0.011482913978397846,
-0.012502092868089676,
0.0755128413438797,
-0.154981330037117,
0.05359124392271042,
0.04910760000348091,
0.024036476388573647,
-0.028148094192147255,
-0.003618486924096942,
0.049692440778017044,
0.0029744624625891447,
-0.0480235293507576,
0.04852555692195892,
0.030332887545228004,
-0.04213590547442436,
-0.0004867148236371577,
-0.005596269853413105,
-0.050171636044979095,
-0.02215643785893917,
-0.002156413858756423,
0.02092478983104229,
-0.005554100498557091,
0.03528625890612602,
0.027474604547023773,
-0.0411539152264595,
-0.002164574107155204,
-0.05746634304523468,
-0.021960770711302757,
-0.021470217034220695,
0.02446777932345867,
0.005445434711873531,
-0.014361180365085602,
-0.04951278865337372,
-0.0006039015715941787,
-0.009327908046543598,
-0.04446231573820114,
0.07385130226612091,
0.03341049328446388,
-0.017068607732653618,
-0.040366433560848236,
0.344733864068985,
0.021833432838320732,
0.038346197456121445,
-0.0706457793712616,
0.035631000995635986,
-0.022191982716321945,
0.005300253629684448,
-0.0435449555516243,
0.0028564734384417534,
-0.0985659584403038,
0.06979276239871979,
0.07068446278572083,
0.001527311047539115,
-0.023341767489910126,
-0.0567413792014122,
-0.09587712585926056,
-0.04134527966380119,
0.04425428435206413,
0.06910212337970734,
0.002958756871521473,
0.023802217096090317,
-0.04142045974731445,
0.05619685724377632,
0.024644188582897186,
-0.004497501999139786,
0.013217492960393429,
-0.004518232773989439,
0.013100546784698963,
0.11506994068622589,
0.03630336746573448,
0.025745974853634834,
-0.002868915908038616,
0.019096700474619865,
-0.02233053930103779,
0.037573009729385376,
0.017332574352622032,
-0.009155955165624619,
0.06975644826889038,
-0.1039915382862091,
-0.03599664941430092,
-0.0064717503264546394,
-0.0031849881634116173,
-0.03261544555425644,
-0.0371839739382267,
-0.07862436026334763,
-0.007860089652240276,
0.10036895424127579,
-0.010263781063258648,
-0.10135076195001602,
-0.015504816547036171,
0.047086555510759354,
0.004538391716778278,
0.055197346955537796,
-0.048692379146814346,
0.07632173597812653,
-0.026085877791047096,
-0.0008663003100082278,
-0.0067824916914105415,
0.009437891654670238,
-0.04269837215542793,
-0.03336043655872345,
-0.04538639634847641,
0.02858847565948963,
-0.07350391149520874,
0.013219711370766163,
0.020771842449903488,
0.021303163841366768,
-0.06096847727894783,
0.025734081864356995,
0.007073558866977692,
-0.019884537905454636,
-0.0014489407185465097,
0.05049067735671997,
-0.027851667255163193,
-0.00957574974745512,
-0.053853098303079605,
-0.007268575485795736,
0.023414667695760727,
0.02173921838402748,
0.00745268864557147,
-0.016886906698346138,
0.06730986386537552,
-0.0883534774184227,
-0.05742613598704338,
-0.01727571152150631,
0.007689516060054302,
0.0521639920771122,
0.004397643264383078,
-0.018420198932290077,
0.016323860734701157,
0.022943731397390366,
0.026147162541747093,
-0.025863666087388992,
-0.056109603494405746,
-0.059706538915634155,
-0.001619628514163196,
-0.014922144822776318,
-0.06895744055509567,
0.11996790766716003,
0.05007600039243698,
0.04998753219842911,
0.04558999091386795,
-0.0059811947867274284,
-0.0016130537260323763,
0.08129564672708511,
-0.009222629480063915,
0.05177991837263107,
-0.02422626130282879,
0.026347124949097633,
0.07885608822107315,
-0.01639896258711815,
-0.007756635081022978,
0.013842685148119926,
-0.004871771205216646,
0.08228296786546707,
-0.04546032100915909,
0.04744207113981247,
0.05624830722808838,
-0.019268764182925224,
0.06997407972812653,
0.011549494229257107,
0.0559970885515213,
-0.06418109685182571,
0.004621279425919056,
-0.30713364481925964,
-0.056173499673604965,
0.012711642310023308,
-0.03722839802503586,
0.0208108089864254,
-0.11148069053888321,
-0.020521171391010284,
-0.027629779651761055,
0.021597212180495262,
-0.01683606021106243,
0.04319857433438301,
0.09167786687612534,
-0.01405861135572195,
-0.028301013633608818,
0.018878648057579994,
0.019352681934833527,
0.060667943209409714,
-0.024105489253997803,
0.030383925884962082,
-0.013579616323113441,
0.018819725140929222,
0.006304997485131025,
0.003320426447317004,
-0.007521852385252714,
0.040870267897844315,
-0.0070145390927791595,
0.06934555619955063,
0.006669287104159594,
0.002354444470256567,
-0.016282295808196068,
0.01817823387682438,
0.023444896563887596,
-0.034009646624326706,
-0.14028958976268768,
0.021947503089904785,
-0.0607420988380909,
0.03134734556078911,
-0.025219770148396492,
0.01768498495221138,
-0.014184237457811832,
-0.08883502334356308,
0.013095122762024403,
-0.012836787849664688,
-0.07030021399259567,
-0.030795631930232048,
-0.007384456694126129,
0.030326085165143013,
0.0312604084610939,
-0.04056379199028015,
0.00008431271271547303,
0.07121285796165466,
-0.01790899597108364,
-0.02848471701145172,
0.00291193975135684,
-0.0009139450849033892,
-0.039347901940345764,
-0.06914821267127991,
-0.005444763693958521,
-0.015964820981025696,
0.0009701379458419979,
0.02734502963721752,
0.03223520889878273,
0.018424928188323975,
-0.0054906136356294155,
-0.02277316339313984,
0.003443294670432806,
0.004869021940976381,
-0.002479606308043003,
0.026959622278809547,
0.05559277534484863,
0.011156400665640831,
0.0317469947040081,
-0.01814635843038559,
0.05138871446251869,
0.007955357432365417,
-0.04876144975423813,
-0.053466249257326126,
-0.04525414854288101,
-0.04696742817759514,
0.04122031480073929,
-0.016089072450995445,
-0.026858186349272728,
0.02273162640631199,
-0.02092338167130947,
-0.013629776425659657,
0.02071361057460308,
0.01921638660132885,
-0.04906227067112923,
0.04957268759608269,
0.026400605216622353,
0.010136960074305534,
-0.012397765181958675,
-0.015473899431526661,
-0.0033012263011187315,
0.05397860333323479,
0.0121053671464324,
-0.27620264887809753,
-0.05410480871796608,
0.045696940273046494,
0.012009833008050919,
-0.03652023896574974,
0.014436263591051102,
-0.011112913489341736,
-0.010451341979205608,
-0.04518570750951767,
-0.013897345401346684,
-0.02450719103217125,
0.05463607236742973,
0.030316440388560295,
0.07898034155368805,
-0.039055824279785156,
-0.037692178040742874,
0.02814682014286518,
-0.0010049414122477174,
0.0014152806252241135,
-0.0655893012881279,
0.028037937358021736,
-0.04804445803165436,
0.14010651409626007,
0.009807825088500977,
0.009827791713178158,
0.059455592185258865,
0.0645655170083046,
0.022160600870847702,
-0.008591917343437672,
0.018160764127969742,
-0.054924145340919495,
0.014341667294502258,
0.08296267688274384,
0.004943700972944498,
-0.0346280001103878,
0.09015747159719467,
-0.03723714500665665,
0.015469335950911045,
0.007888979278504848,
0.039965495467185974,
-0.033886607736349106,
0.03160419315099716,
-0.07257845997810364,
-0.011934829875826836,
0.062015097588300705,
-0.0834541767835617,
-0.041526999324560165,
-0.01412266120314598,
-0.00733480229973793,
0.04576797038316727,
-0.04294914752244949,
-0.010092035867273808,
0.03326476737856865,
0.020893177017569542,
0.029365604743361473,
-0.0016782915918156505,
0.02838766761124134,
-0.008411693386733532,
0.004582805093377829,
0.014578225091099739,
-0.05714414641261101,
-0.0008197813294827938,
-0.048795800656080246,
0.09114419668912888,
0.046167388558387756
] |
PMID:8341 | Effects of autonomic drugs on epididymal contractions. | The spontaneous contractility of rat epididymis was recorded in vivo and the effects of various autonomic drugs were studied. Norepinephrine, epinephrine, and orciprenaline produced a sudden increase in tonus and in the size and frequency of epididymal contractions. Phentolamine (an alpha-blocker agent) inhibited the effects of norepinephrine. On the other hand, alprenolol (a beta-blocker agent) inhibited the effects of orciprenaline but did not block the effects of norepinephrine. In addition, phentolamine and alprenolol decreased the spontaneous activity of the epididymis. Acetylcholine produced effects similar to those of norepinephrine. These effects were blocked by atropine. The results described would indicate the presence of the two receptors, alpha and beta, and that both are mediators of stimulatory effects. | Effects of autonomic drugs on epididymal contractions. The spontaneous contractility of rat epididymis was recorded in vivo and the effects of various autonomic drugs were studied. Norepinephrine, epinephrine, and orciprenaline produced a sudden increase in tonus and in the size and frequency of epididymal contractions. Phentolamine (an alpha-blocker agent) inhibited the effects of norepinephrine. On the other hand, alprenolol (a beta-blocker agent) inhibited the effects of orciprenaline but did not block the effects of norepinephrine. In addition, phentolamine and alprenolol decreased the spontaneous activity of the epididymis. Acetylcholine produced effects similar to those of norepinephrine. These effects were blocked by atropine. The results described would indicate the presence of the two receptors, alpha and beta, and that both are mediators of stimulatory effects. | [
-0.041795771569013596,
-0.04264579340815544,
0.06335923075675964,
0.03404076397418976,
-0.009842265397310257,
0.01008085161447525,
0.053400918841362,
0.025637425482273102,
-0.03155115246772766,
-0.07665048539638519,
-0.010280769318342209,
0.026252446696162224,
-0.03298141807317734,
0.06479603052139282,
-0.006358119659125805,
-0.00857515912503004,
-0.0005632814718410373,
0.00788420345634222,
-0.08838079869747162,
-0.04335606098175049,
-0.014684361405670643,
-0.007187516428530216,
0.030397163704037666,
0.03112075664103031,
0.04221406206488609,
-0.007076960057020187,
-0.021943392232060432,
-0.015434985980391502,
-0.05434373393654823,
-0.1611190140247345,
0.010265899822115898,
-0.002645206870511174,
0.029289118945598602,
0.024337077513337135,
0.00852085929363966,
-0.02383008599281311,
-0.020159883424639702,
0.054339583963155746,
-0.00426351698115468,
0.00997038371860981,
0.018108278512954712,
0.004783544689416885,
-0.009820541366934776,
0.041558701545000076,
0.035112328827381134,
0.022278811782598495,
0.014530261978507042,
-0.05463942512869835,
0.10574554651975632,
0.011416804045438766,
0.05250943452119827,
-0.02206888422369957,
0.007213615346699953,
0.053498171269893646,
0.013513325713574886,
-0.03884269297122955,
-0.02531568892300129,
0.054369281977415085,
0.04366401210427284,
0.0957314521074295,
-0.00009918103751260787,
0.06565217673778534,
-0.13511979579925537,
0.06359778344631195,
0.020634926855564117,
0.009117194451391697,
-0.006529280450195074,
0.05075373873114586,
0.035630907863378525,
0.06953996419906616,
-0.04466608911752701,
0.05940111353993416,
-0.02330991066992283,
-0.035571418702602386,
0.021030107513070107,
-0.045135416090488434,
-0.013814644888043404,
-0.04932689666748047,
0.038859762251377106,
0.0008766637183725834,
0.024303510785102844,
-0.0605599507689476,
-0.03016585297882557,
-0.027179298922419548,
0.0010829782113432884,
-0.023066764697432518,
0.046094633638858795,
-0.03401724994182587,
-0.039537351578474045,
-0.04034285247325897,
-0.009719186462461948,
0.0012087259674444795,
-0.038514696061611176,
-0.011527652852237225,
-0.016108835116028786,
-0.007257755845785141,
0.002300430554896593,
-0.01671961508691311,
-0.05265103653073311,
0.3393045663833618,
0.012251315638422966,
0.0375295951962471,
-0.05909568443894386,
-0.02215254120528698,
0.022560682147741318,
0.010829710401594639,
-0.022788742557168007,
-0.05072538182139397,
-0.04576818272471428,
0.06656850874423981,
0.11040297895669937,
0.01239396259188652,
0.025788776576519012,
-0.03606417402625084,
-0.01929127238690853,
-0.0663735643029213,
0.06376371532678604,
0.02998279221355915,
0.026862550526857376,
0.03261497616767883,
-0.07182371616363525,
-0.04413769394159317,
0.0056681325659155846,
0.04375818744301796,
0.0041285366751253605,
-0.0017889289883896708,
-0.013296102173626423,
0.1020127460360527,
0.030532609671354294,
-0.0006007099873386323,
0.058909822255373,
0.02333810180425644,
-0.022544225677847862,
0.03043517656624317,
0.037330396473407745,
-0.008572758175432682,
0.03241916000843048,
-0.00586326839402318,
0.02440514974296093,
-0.05592012032866478,
-0.009310037828981876,
-0.067583829164505,
-0.018221361562609673,
-0.1232881247997284,
-0.04469321668148041,
0.10448603332042694,
0.03911544755101204,
0.004678636789321899,
-0.05995612218976021,
0.07950309664011002,
-0.05537460371851921,
-0.012858359143137932,
-0.004354776348918676,
0.06527287513017654,
0.013890848495066166,
0.05856094881892204,
0.007529835216701031,
-0.040405772626399994,
-0.01819397322833538,
-0.05390692874789238,
-0.029723569750785828,
-0.017049895599484444,
0.0016373838298022747,
0.06360981613397598,
0.00047418547910638154,
0.01670333743095398,
-0.038357026875019073,
0.02670670859515667,
-0.039194975048303604,
-0.02603628858923912,
0.0036909624468535185,
-0.013967923820018768,
-0.03564668819308281,
-0.040014997124671936,
-0.04717155918478966,
0.017035912722349167,
0.05432649701833725,
-0.05070239678025246,
0.0257842019200325,
-0.04497208446264267,
0.03987407311797142,
0.003960269503295422,
0.013247784227132797,
-0.00953073799610138,
-0.00994428712874651,
-0.028022760525345802,
-0.028785033151507378,
0.00033597752917557955,
0.003568517044186592,
-0.0015679014613851905,
0.022544778883457184,
-0.0343090295791626,
-0.07403609156608582,
-0.01070612296462059,
-0.050993431359529495,
-0.01841610111296177,
0.011607090011239052,
-0.012652941048145294,
-0.034671422094106674,
-0.026353877037763596,
0.07093148678541183,
0.02970919758081436,
0.030774718150496483,
0.052074823528528214,
0.03238048031926155,
0.0723457932472229,
-0.04869648069143295,
-0.0037514984142035246,
0.09488366544246674,
-0.03351657837629318,
0.008316369727253914,
-0.0013611525064334273,
0.06641202419996262,
-0.00805575866252184,
-0.04458250850439072,
0.06868930160999298,
-0.054715175181627274,
0.04004953056573868,
0.08701453357934952,
-0.0736740380525589,
0.050220683217048645,
0.03219875320792198,
0.02413584291934967,
-0.2964111566543579,
-0.024012237787246704,
0.009607777930796146,
-0.06880638748407364,
0.07347020506858826,
-0.08594551682472229,
0.05172508582472801,
-0.05013490840792656,
0.020795248448848724,
-0.07163792103528976,
0.032428544014692307,
0.05200550705194473,
-0.010462835431098938,
0.021710272878408432,
-0.012526260688900948,
0.0654100775718689,
0.007614749483764172,
-0.03605932369828224,
0.03795071318745613,
0.03707123547792435,
-0.0014498544624075294,
-0.0024421142879873514,
-0.05071036517620087,
0.021782726049423218,
-0.022902796044945717,
-0.04864002764225006,
0.06085357442498207,
-0.0075282929465174675,
-0.04889988899230957,
-0.02177274413406849,
-0.003920072223991156,
0.07507351785898209,
-0.020272012799978256,
-0.11139735579490662,
-0.020387310534715652,
-0.025723837316036224,
0.06515626609325409,
0.019634529948234558,
0.0317980982363224,
-0.040836989879608154,
-0.0729234591126442,
0.004851976875215769,
0.009948421269655228,
-0.012607721611857414,
-0.05139392614364624,
-0.04036330804228783,
0.025189856067299843,
0.026927420869469643,
-0.061034027487039566,
0.00932888314127922,
-0.008960417471826077,
-0.01460699737071991,
-0.004720374476164579,
-0.03557029739022255,
0.021964985877275467,
-0.0003587016835808754,
-0.11520559340715408,
0.037414927035570145,
-0.03446555882692337,
0.0037171514704823494,
0.056262269616127014,
-0.0036869936157017946,
0.0068366434425115585,
-0.05569161847233772,
0.03225070610642433,
0.00228572404012084,
0.057643745094537735,
-0.03961605206131935,
0.044568710029125214,
0.029281385242938995,
0.054065171629190445,
0.16484180092811584,
-0.04559851065278053,
0.011771896854043007,
0.06874938309192657,
-0.01488132867962122,
-0.007238721940666437,
0.00802633073180914,
-0.060851551592350006,
0.022882990539073944,
-0.03230836242437363,
-0.04947700351476669,
0.05440189316868782,
0.0077459621243178844,
-0.03518000990152359,
-0.05220596119761467,
0.021175792440772057,
-0.0012053997488692403,
0.033720482140779495,
-0.03953513503074646,
-0.022651631385087967,
0.03228873014450073,
-0.025894662365317345,
0.023809287697076797,
0.06357692182064056,
0.046697165817022324,
-0.26202458143234253,
-0.02113962732255459,
0.05445075035095215,
-0.01199943944811821,
-0.05356089770793915,
0.05776457116007805,
-0.009552063420414925,
-0.04977891966700554,
-0.06725574284791946,
0.017403561621904373,
-0.02242313325405121,
-0.015205451287329197,
0.06106692552566528,
0.007361389230936766,
-0.06595609337091446,
0.05522390455007553,
0.04407379403710365,
0.02606971375644207,
0.059730470180511475,
-0.05686694011092186,
-0.02084207348525524,
-0.01676029898226261,
0.1506529152393341,
-0.009980558417737484,
-0.002187714446336031,
0.08068785071372986,
0.003597509814426303,
0.0005505518638528883,
-0.03712951019406319,
0.016161387786269188,
-0.008147848770022392,
-0.005491252988576889,
0.05445215478539467,
-0.05206960067152977,
0.030173717066645622,
0.004522099159657955,
-0.05327172949910164,
0.011845570988953114,
-0.022352011874318123,
0.006630209740251303,
-0.04249242693185806,
0.04309575632214546,
0.0016941279172897339,
-0.004161789547652006,
0.013708733953535557,
-0.033865999430418015,
-0.022338872775435448,
0.013800733722746372,
-0.010023389011621475,
0.05984091758728027,
-0.000008726019586902112,
-0.023419978097081184,
0.009540279395878315,
0.07760925590991974,
-0.011960742995142937,
-0.003517348784953356,
-0.05312534049153328,
0.025075163692235947,
0.0698111429810524,
0.0085680540651083,
-0.02687585912644863,
-0.05910457298159599,
-0.022111549973487854,
-0.006529536098241806,
0.07763063907623291
] |
PMID:8343 | [Studies of various factors in thyrotropoin releasing hromone TRH) radioimmunoassay for serum (author's transl)]. | To study the significance of TRH in the hypothalamo-pituitary-thyroid axis measurement of TRH in body fluid are needed. We previously reported TRH radioimmunoassay for urine. TRH radioimmunoassay for serum has not established yet, because TRH immunoreactivity is inactivated with serum. We investigated effects of various factors on this inactivation and method for prevention of this inactivation. Synthetic TRH was added to normal human serum at 4 degrees C and incubated at 60 degrees C, 37 degrees C, 20 degrees C, 4 degrees C or -20 degrees C for various intervals. After incubation, recovery of TRH was measured. After one hour incubation, recovery of TRH was 9.2% at 37 degrees C, 34.5% at 20 degrees C, 100% at 4 degrees C or -20 degrees C. Incubation of TRH serum mixtures at 65 degrees C after incubation at 37 degrees C resulted in some recovery of TRH. After one hour incubation at 37 degrees C, recovery of TRH was 9.2% at serum pH 7.0, 100% at serum pH 3.0 to 5.0 or 11.0. Recovery of TRH was increased in accordance with stepwisely increase of serum dilution. Concentrations of serum thyroid hormone did not affect recovery of TRH. Smaller quantities of TRH were more rapidly inactivated. Inactivation of TRH immunoreactivity could be prevented addition of BAL (over 0.25 mg/ml) or mixture of 8-Hydroxyquinoline (HQ) and Tween 20(T) (over 0.1 mg/ml of HQ and 1 lmg/ml of T). Duration of effectiveness of BAL was short. Effectiveness of HQT continued for 12 weeks, if HQT treated serum was stored at -20 degrees C. From above data it was suggested that TRH immunoreactivity might be inactivated with enzyme system and other factors and TRH levels in the serum might be able to measure with addition of HQT to serum. | [Studies of various factors in thyrotropoin releasing hromone TRH) radioimmunoassay for serum (author's transl)]. To study the significance of TRH in the hypothalamo-pituitary-thyroid axis measurement of TRH in body fluid are needed. We previously reported TRH radioimmunoassay for urine. TRH radioimmunoassay for serum has not established yet, because TRH immunoreactivity is inactivated with serum. We investigated effects of various factors on this inactivation and method for prevention of this inactivation. Synthetic TRH was added to normal human serum at 4 degrees C and incubated at 60 degrees C, 37 degrees C, 20 degrees C, 4 degrees C or -20 degrees C for various intervals. After incubation, recovery of TRH was measured. After one hour incubation, recovery of TRH was 9.2% at 37 degrees C, 34.5% at 20 degrees C, 100% at 4 degrees C or -20 degrees C. Incubation of TRH serum mixtures at 65 degrees C after incubation at 37 degrees C resulted in some recovery of TRH. After one hour incubation at 37 degrees C, recovery of TRH was 9.2% at serum pH 7.0, 100% at serum pH 3.0 to 5.0 or 11.0. Recovery of TRH was increased in accordance with stepwisely increase of serum dilution. Concentrations of serum thyroid hormone did not affect recovery of TRH. Smaller quantities of TRH were more rapidly inactivated. Inactivation of TRH immunoreactivity could be prevented addition of BAL (over 0.25 mg/ml) or mixture of 8-Hydroxyquinoline (HQ) and Tween 20(T) (over 0.1 mg/ml of HQ and 1 lmg/ml of T). Duration of effectiveness of BAL was short. Effectiveness of HQT continued for 12 weeks, if HQT treated serum was stored at -20 degrees C. From above data it was suggested that TRH immunoreactivity might be inactivated with enzyme system and other factors and TRH levels in the serum might be able to measure with addition of HQT to serum. | [
0.025143297389149666,
0.037667568773031235,
0.021914487704634666,
0.04670465365052223,
-0.021042313426733017,
0.003710957244038582,
0.07623849809169769,
0.03832339122891426,
-0.0768878236413002,
-0.04393200948834419,
0.0008237233851104975,
-0.08442909270524979,
0.044487364590168,
0.04649990797042847,
-0.03036540001630783,
-0.017522398382425308,
0.0847514346241951,
-0.03776371851563454,
-0.028875302523374557,
0.020742401480674744,
-0.030226988717913628,
-0.01805899292230606,
0.014231421984732151,
0.04305598884820938,
0.06361854821443558,
0.06391683220863342,
-0.07243411988019943,
0.02238498255610466,
-0.02358313836157322,
-0.14813944697380066,
0.05041014030575752,
-0.010508697479963303,
-0.00785783026367426,
-0.020722651854157448,
-0.012434137985110283,
0.04810699075460434,
-0.013641546480357647,
-0.0225126501172781,
0.030426178127527237,
0.04162251576781273,
0.02124900557100773,
0.013440057635307312,
-0.03813435882329941,
-0.008109181188046932,
0.032771702855825424,
0.06099927797913551,
-0.039618369191884995,
0.05801896005868912,
0.07815085351467133,
0.0749182179570198,
-0.008296502754092216,
0.011955393478274345,
0.01855350472033024,
0.11188052594661713,
0.04172186180949211,
-0.01438525877892971,
0.020200228318572044,
0.03844869136810303,
-0.011927356012165546,
-0.006732343230396509,
0.022359957918524742,
0.10919377207756042,
-0.12144602090120316,
0.05792822688817978,
0.041476331651210785,
0.01881730742752552,
0.026276905089616776,
-0.03440140560269356,
0.05164695158600807,
0.03679893538355827,
-0.022207055240869522,
0.03381757065653801,
0.02498726360499859,
0.011126721277832985,
-0.0178900845348835,
-0.03476367145776749,
0.007039290852844715,
-0.04452928900718689,
0.03507160767912865,
0.026620136573910713,
-0.018978020176291466,
0.019447125494480133,
0.053311001509428024,
0.016413522884249687,
-0.00041805539513006806,
-0.052061229944229126,
0.009859619662165642,
0.008223133161664009,
0.007558367680758238,
0.019233308732509613,
0.013428542762994766,
-0.026077255606651306,
-0.0442778579890728,
0.037774596363306046,
-0.030001988634467125,
0.004826403222978115,
0.02575775235891342,
-0.06091323867440224,
0.005052123684436083,
0.3489241898059845,
0.010596608743071556,
0.025953540578484535,
-0.1224663257598877,
-0.06345163285732269,
0.03827641159296036,
-0.011021324433386326,
-0.02911112643778324,
0.02230176515877247,
-0.04792393743991852,
0.03956135734915733,
-0.0020998867694288492,
-0.0071085374802351,
0.032288603484630585,
-0.09543709456920624,
0.028823714703321457,
-0.01055640634149313,
0.047609515488147736,
0.05077558010816574,
-0.016787609085440636,
0.011260654777288437,
0.008768009021878242,
0.032720621675252914,
0.07773835211992264,
-0.09413110464811325,
-0.014183047227561474,
0.03211777284741402,
0.06832679361104965,
0.12196068465709686,
0.014767526648938656,
0.0032855623867362738,
0.026566453278064728,
-0.02898373082280159,
-0.009725857526063919,
0.02367531508207321,
0.04470133036375046,
0.036165960133075714,
0.028307827189564705,
-0.08200184255838394,
-0.032512739300727844,
0.010772212408483028,
0.013867625035345554,
-0.09183600544929504,
-0.024640757590532303,
-0.050805628299713135,
-0.025253716856241226,
0.10173196345567703,
-0.027306722477078438,
0.004471259191632271,
0.019832218065857887,
-0.06620651483535767,
-0.007633364759385586,
0.05401464179158211,
-0.03652079030871391,
0.016732072457671165,
-0.014996957965195179,
0.04033819958567619,
-0.043759267777204514,
0.029132172465324402,
-0.02087966352701187,
0.030693409964442253,
-0.0899326503276825,
-0.04083370044827461,
-0.059264957904815674,
-0.005026967264711857,
-0.009415963664650917,
-0.035988107323646545,
0.022896332666277885,
-0.004320188891142607,
0.03534216433763504,
0.022847944870591164,
-0.0026229519862681627,
-0.010921788401901722,
-0.03221184015274048,
-0.01704135350883007,
0.03996926546096802,
-0.03004172258079052,
0.01493352372199297,
-0.012050723657011986,
-0.006010294426232576,
-0.010025757364928722,
-0.01062940713018179,
-0.030445830896496773,
-0.019985085353255272,
-0.005640669260174036,
0.03247387707233429,
-0.022440608590841293,
-0.041766513139009476,
0.04682939499616623,
0.011980533599853516,
0.06340346485376358,
-0.03447800502181053,
-0.04656718671321869,
-0.07722855359315872,
-0.018256178125739098,
0.010903723537921906,
-0.0657438412308693,
-0.042426638305187225,
0.03230633959174156,
0.02526724524796009,
0.012284567579627037,
0.06515607982873917,
-0.007961569353938103,
0.03419618308544159,
0.03160542622208595,
0.034812260419130325,
0.07749253511428833,
0.02493221126496792,
-0.03819499537348747,
0.07831457257270813,
-0.022128133103251457,
-0.015421729534864426,
0.03078179433941841,
-0.05909473076462746,
-0.009091908112168312,
-0.030573926866054535,
0.02738841623067856,
0.04054034501314163,
-0.02174457721412182,
0.0023264652118086815,
0.013266392052173615,
0.06631254404783249,
-0.030755093321204185,
-0.02474110759794712,
-0.3322361707687378,
-0.028761114925146103,
0.028415540233254433,
0.0032738959416747093,
0.020112399011850357,
-0.11863614618778229,
-0.06603188812732697,
-0.004449330270290375,
0.03765556216239929,
-0.061643507331609726,
0.07152271270751953,
0.13171403110027313,
-0.09170369058847427,
-0.07974964380264282,
0.018442202359437943,
0.026049189269542694,
0.015864096581935883,
0.00019931043789256364,
0.027846312150359154,
-0.044165272265672684,
-0.011342672631144524,
-0.004054470453411341,
-0.05312233790755272,
-0.010569577105343342,
0.028801381587982178,
-0.028431076556444168,
0.06451109051704407,
-0.03988462686538696,
-0.04155439883470535,
-0.01913696900010109,
0.010319114662706852,
0.02076050080358982,
0.021871816366910934,
-0.08290217071771622,
0.01537700742483139,
-0.031501755118370056,
-0.05520205199718475,
0.005851333495229483,
-0.03247884288430214,
0.0009884138125926256,
-0.060518257319927216,
-0.03224565461277962,
-0.0012055870611220598,
-0.062452852725982666,
-0.04750413820147514,
0.021809853613376617,
-0.033588118851184845,
0.011843672022223473,
-0.015425981022417545,
-0.01708291657269001,
0.05677692964673042,
-0.02189982682466507,
0.026594053953886032,
-0.00505650183185935,
0.0007990073063410819,
0.0034274363424628973,
-0.05929096043109894,
-0.02141406387090683,
-0.03805528208613396,
0.021502995863556862,
0.0006611696444451809,
0.06269125640392303,
-0.0031969372648745775,
0.01598295569419861,
-0.023520570248365402,
-0.047376178205013275,
-0.01613958552479744,
0.03568302467465401,
0.09970852732658386,
0.06051459535956383,
-0.02333551086485386,
0.07326901704072952,
-0.028882808983325958,
0.0458110012114048,
0.009699179790914059,
-0.046701401472091675,
-0.04298285394906998,
-0.05685167759656906,
-0.012512787245213985,
0.0063539291732013226,
-0.0013753618113696575,
-0.05237706005573273,
0.05398678407073021,
0.03303269296884537,
-0.03341875597834587,
0.06146914139389992,
0.047323450446128845,
-0.001610935083590448,
0.0516447052359581,
0.012046686373651028,
-0.04346240311861038,
0.04550573602318764,
0.013619644567370415,
-0.05355619266629219,
-0.0070899473503232,
0.007565496955066919,
-0.2945387661457062,
-0.028630085289478302,
-0.014509488828480244,
-0.04136083275079727,
0.01870328187942505,
0.01602538488805294,
0.0014878124929964542,
0.022993281483650208,
-0.04307980090379715,
0.01579282619059086,
-0.04880069941282272,
0.05150952935218811,
0.06974421441555023,
0.01734473742544651,
-0.06794293224811554,
-0.011848599649965763,
0.025903873145580292,
0.005237134639173746,
-0.0367048904299736,
-0.035140205174684525,
0.025027750059962273,
-0.031361620873212814,
0.1056961938738823,
0.010233135893940926,
0.01640930585563183,
0.06622244417667389,
0.0691409558057785,
0.048679858446121216,
0.009054942987859249,
-0.03690413013100624,
-0.0410713292658329,
-0.017381662502884865,
0.012066951021552086,
0.007278799545019865,
0.027303151786327362,
0.0037753558717668056,
-0.024523358792066574,
-0.0007186730508692563,
0.000055178294132929295,
0.03593849390745163,
-0.013322950340807438,
0.007528755348175764,
-0.0315622016787529,
-0.04719119891524315,
0.06972116231918335,
-0.07340893149375916,
-0.02353246882557869,
-0.04803222045302391,
0.027678662911057472,
0.028029652312397957,
-0.040289152413606644,
-0.0003727155562955886,
0.00023698669974692166,
0.019298609346151352,
0.020711978897452354,
0.020617786794900894,
0.022919338196516037,
-0.0010967981070280075,
-0.018144410103559494,
-0.006053555756807327,
-0.0544947013258934,
-0.03328751400113106,
-0.014837938360869884,
0.07256186008453369,
0.01420614868402481
] |
PMID:8344 | [Cutaneous side effects of systemic drugs. Part 4 of a synopsis. 6/7. Drugs affecting the central nervous system. C. Drug-induced photosensitivity. D. Drug-induced changes of skin color]. | This, the fourth part of a synopisis of cutaneous side effects of drugs, covers the drugs affecting the central nervous system: antiepileptics, hypnotics, narcotics and psychopharmaceutics; the myorelaxants and antiallergics follow, and lastly there is a section on drug addiction and placebo. The various cutaneous side effects are listed in chart form referring to more than 500 sources. A drug index is attached for handy reference. The reviews of certain drug induced skin disorders are continued with tables covering photosensitivity and changes in skin colour. Phototoxicity, photoallergy and light sensitivity by porphyria are differentiated. The various pigmentation disorders, colour changes due to metal deposits as well as different localisations are included. | [Cutaneous side effects of systemic drugs. Part 4 of a synopsis. 6/7. Drugs affecting the central nervous system. C. Drug-induced photosensitivity. D. Drug-induced changes of skin color]. This, the fourth part of a synopisis of cutaneous side effects of drugs, covers the drugs affecting the central nervous system: antiepileptics, hypnotics, narcotics and psychopharmaceutics; the myorelaxants and antiallergics follow, and lastly there is a section on drug addiction and placebo. The various cutaneous side effects are listed in chart form referring to more than 500 sources. A drug index is attached for handy reference. The reviews of certain drug induced skin disorders are continued with tables covering photosensitivity and changes in skin colour. Phototoxicity, photoallergy and light sensitivity by porphyria are differentiated. The various pigmentation disorders, colour changes due to metal deposits as well as different localisations are included. | [
0.0038281171582639217,
-0.04324541240930557,
0.04713305085897446,
0.04249226301908493,
0.034794554114341736,
-0.005415414925664663,
0.0841001644730568,
0.04064737632870674,
-0.06279484182596207,
-0.02613797038793564,
0.018028270453214645,
0.0319284051656723,
0.003632541513070464,
0.06834017485380173,
-0.007186648901551962,
-0.0030668997205793858,
0.0446801483631134,
-0.046484339982271194,
-0.03594588488340378,
-0.014227007515728474,
-0.02885235846042633,
0.00958717055618763,
0.0008514778455719352,
-0.029562855139374733,
0.0004276348336134106,
-0.024985605850815773,
0.0005522019928321242,
-0.0016145207919180393,
-0.08175361901521683,
-0.16021059453487396,
0.025590064004063606,
0.07283828407526016,
-0.01909252069890499,
-0.054744962602853775,
-0.036456380039453506,
-0.046960823237895966,
-0.021875819191336632,
0.0020932171028107405,
0.0210402961820364,
-0.0015724925324320793,
0.05063936114311218,
0.0019504767842590809,
-0.030179860070347786,
0.04518367350101471,
0.0016120604705065489,
-0.04778271168470383,
-0.03468243405222893,
0.032067038118839264,
0.10995583981275558,
-0.055137988179922104,
0.007469990290701389,
-0.06805763393640518,
0.046305958181619644,
0.08071871846914291,
-0.005310260225087404,
-0.015774821862578392,
-0.011106486432254314,
0.0388537272810936,
-0.02615443244576454,
0.039134204387664795,
0.021564684808254242,
0.09182817488908768,
-0.11177026480436325,
0.01883755810558796,
-0.005100299138575792,
-0.041755370795726776,
0.025199629366397858,
-0.03541228547692299,
0.04600868374109268,
0.038925476372241974,
-0.04129698500037193,
0.02016139216721058,
-0.008952605538070202,
0.013961969874799252,
-0.026669148355722427,
-0.03365381062030792,
-0.028384078294038773,
-0.04774777963757515,
-0.04648054018616676,
-0.02448761649429798,
0.03936395049095154,
0.007585940882563591,
0.011026982218027115,
-0.039692964404821396,
0.0721026360988617,
-0.00866677612066269,
0.026872115209698677,
-0.012029756791889668,
-0.03012530691921711,
-0.011728025041520596,
0.002306591486558318,
-0.056411147117614746,
0.02826426923274994,
-0.032436687499284744,
-0.03317262604832649,
-0.036244917660951614,
-0.02494729682803154,
-0.02268259786069393,
0.001976732863113284,
0.33904165029525757,
0.0000993166831904091,
0.015037382952868938,
-0.006212552078068256,
0.024997850880026817,
0.0032767653465270996,
0.029053134843707085,
0.06672340631484985,
-0.025739019736647606,
-0.06371122598648071,
0.013804079033434391,
0.0266225915402174,
-0.017926104366779327,
0.00884269829839468,
0.031050806865096092,
-0.040858786553144455,
-0.056270796805620193,
0.0883852019906044,
0.02852397784590721,
0.021862180903553963,
0.03810039535164833,
-0.00839587114751339,
-0.008594511076807976,
0.06366107612848282,
0.02206198126077652,
0.05730307102203369,
0.019138559699058533,
0.012610227800905704,
0.09313412755727768,
0.04391660541296005,
0.01683635078370571,
0.04901178926229477,
-0.007687992881983519,
0.05721520259976387,
0.035140711814165115,
0.04331221431493759,
0.007306467276066542,
0.025721590965986252,
-0.008869050070643425,
0.09227988868951797,
0.02440056763589382,
0.0017066653817892075,
-0.048039212822914124,
0.023525061085820198,
-0.06804908812046051,
-0.0505661703646183,
0.08298681676387787,
0.03788944333791733,
0.015115484595298767,
-0.031115813180804253,
0.06568838655948639,
-0.024379396811127663,
0.04180554673075676,
-0.017638079822063446,
0.12659896910190582,
-0.1058088168501854,
0.03693957254290581,
-0.017129721119999886,
-0.043169695883989334,
0.021810974925756454,
0.022506583482027054,
-0.012799886986613274,
-0.036110781133174896,
-0.016351334750652313,
0.12048809975385666,
0.006869228091090918,
-0.028340518474578857,
-0.045928917825222015,
0.01075386069715023,
-0.0605543851852417,
-0.008315229788422585,
0.006033626385033131,
0.040632788091897964,
-0.019314657896757126,
0.004453232511878014,
-0.03472256660461426,
0.0038027388509362936,
0.04386771470308304,
0.011422446928918362,
0.019374821335077286,
-0.02584492415189743,
0.030586153268814087,
0.06936784088611603,
-0.030751721933484077,
0.012410862371325493,
-0.04262683913111687,
-0.013082913123071194,
-0.03334537148475647,
0.08036606758832932,
0.05309591814875603,
-0.009999235160648823,
-0.05232347175478935,
-0.041156068444252014,
-0.07377442717552185,
0.0010680744890123606,
-0.01750539056956768,
-0.0250514168292284,
-0.040309417992830276,
-0.07870840281248093,
-0.030575644224882126,
-0.014431475661695004,
0.06848259270191193,
0.022503307089209557,
0.04794015362858772,
0.08470044285058975,
0.025767963379621506,
0.0408494770526886,
-0.05784027278423309,
0.03098081238567829,
0.05478668585419655,
-0.03685769811272621,
-0.04684637859463692,
0.06970009207725525,
0.05306738242506981,
-0.039963364601135254,
-0.017223650589585304,
-0.0297663826495409,
0.006379968486726284,
0.03591609001159668,
0.0423169881105423,
0.05027604475617409,
0.01632959395647049,
-0.04083830863237381,
-0.08431944996118546,
-0.3037709593772888,
0.041725460439920425,
-0.0042749070562422276,
-0.01930602826178074,
-0.006544212345033884,
0.003251452697440982,
-0.004356569144874811,
-0.005256291013211012,
0.013023433275520802,
0.026441678404808044,
0.07669642567634583,
0.023254122585058212,
0.010493558831512928,
-0.07969646155834198,
-0.02144855074584484,
-0.02886991575360298,
0.037925589829683304,
-0.05164620652794838,
0.007074797060340643,
-0.05059207230806351,
-0.031246474012732506,
-0.02064046449959278,
0.0011730232508853078,
0.012777740135788918,
-0.0028328290209174156,
0.025457436218857765,
0.1171286329627037,
0.017020026221871376,
-0.08498251438140869,
-0.07151351869106293,
-0.027673887088894844,
0.05053338780999184,
-0.03640368953347206,
-0.09288077056407928,
0.030141297727823257,
-0.019063321873545647,
-0.02400106005370617,
-0.006639092694967985,
-0.054658059030771255,
-0.024394137784838676,
0.026330696418881416,
0.022409439086914062,
-0.0258379764854908,
0.02494816482067108,
-0.05056534335017204,
0.02001057006418705,
0.018851909786462784,
0.02706233412027359,
0.0017543155699968338,
-0.006623902823776007,
-0.0018646775279194117,
0.0304438266903162,
-0.02871587686240673,
0.007864858955144882,
0.045777637511491776,
-0.05243447422981262,
-0.09046001732349396,
-0.015598723664879799,
-0.052867092192173004,
-0.013059641234576702,
0.008862992748618126,
-0.013609914109110832,
0.06761609762907028,
-0.06738550961017609,
-0.0010937011102214456,
-0.03486565127968788,
-0.049947645515203476,
-0.04957316070795059,
0.0684114322066307,
0.029057426378130913,
-0.02539556659758091,
0.18821488320827484,
-0.0059682633727788925,
-0.039355821907520294,
0.06120210513472557,
0.10296832025051117,
0.0035435070749372244,
0.014729202724993229,
-0.04121055454015732,
0.0645158588886261,
-0.05736645311117172,
-0.0077608488500118256,
-0.01816134713590145,
-0.005055177491158247,
-0.0035835024900734425,
-0.0243387371301651,
0.04199294373393059,
-0.03445745259523392,
0.06513088196516037,
-0.04130883887410164,
0.0015475035179406404,
0.02988133765757084,
-0.010828021913766861,
-0.025891054421663284,
0.039792608469724655,
0.03743840381503105,
-0.23905359208583832,
-0.0025047019589692354,
0.020872509106993675,
0.02925706095993519,
0.02579060196876526,
0.03674619272351265,
0.019465507939457893,
-0.014733799733221531,
0.0072488933801651,
-0.03580018877983093,
0.0061142705380916595,
0.00010337775165680796,
0.04510967805981636,
0.009225995279848576,
-0.05172077566385269,
-0.019752545282244682,
0.08732738345861435,
-0.027276214212179184,
-0.004665795713663101,
0.013123413547873497,
-0.015579655766487122,
-0.012682459317147732,
0.15337085723876953,
0.014603660441935062,
-0.010264558717608452,
0.07401935756206512,
-0.03452898934483528,
0.03486894071102142,
-0.016311082988977432,
0.035625383257865906,
0.012826076708734035,
0.00844348780810833,
0.021725745871663094,
0.03463517874479294,
0.004208549857139587,
0.02688564546406269,
-0.05583897978067398,
-0.03974233567714691,
0.020878437906503677,
-0.021601062268018723,
-0.00852261297404766,
-0.03882138058543205,
-0.05204058811068535,
-0.0043767779134213924,
0.08061421662569046,
-0.04508557543158531,
-0.07207020372152328,
-0.05317848548293114,
0.03375353664159775,
-0.002291532466188073,
-0.05213112384080887,
0.0008171307854354382,
0.0002760485513135791,
0.035506971180438995,
0.004304701928049326,
-0.06104357913136482,
-0.03694179281592369,
-0.03577800467610359,
0.032819319516420364,
0.0022424254566431046,
-0.04667648673057556,
-0.07392553240060806,
-0.08724657446146011,
-0.021542152389883995,
0.06593699753284454
] |
PMID:8356 | Gastric and extragastric gastrin release in normal subjects in duodenal ulcer patients, and in patients with partial gastrectomy (Billroth I). | In 10 normal subjects, in 32 patients with duodenal ulcer (DU), and in 11 patients with partial gastrectomy (Billroth I), serum gastrin rose significantly after an oral and intraduodenal test meal. The highest increases were observed in DU patients after the oral as well as after the intraduodenal test meal. After the intraduodenal test meal in 4 normal subjects and in 17 DU patients an increase of gastric acid secretion and serum gastrin was measured. In basal state, after an intraduodenal or an oral test meal, DU patients with normal gastric acid secretory capacity had higher serum gastrin concentrations than DU patients with gastric hypersecretion. There was a good correlation between peak serum gastrin levels after the oral and after the intraduodenal test meal. From these data it is concluded: (1) Intraduodenal application of a test meal results in release of gastrin from extragastric sites. (2) Extragastric gastrin is biologically active. (3) DU patients are able to release more antral and more extragastric gastrin in response to a test meal. Further studies, however, are necessary to show the significance of these findings in the pathogenesis of peptic ulcer disease. | Gastric and extragastric gastrin release in normal subjects in duodenal ulcer patients, and in patients with partial gastrectomy (Billroth I). In 10 normal subjects, in 32 patients with duodenal ulcer (DU), and in 11 patients with partial gastrectomy (Billroth I), serum gastrin rose significantly after an oral and intraduodenal test meal. The highest increases were observed in DU patients after the oral as well as after the intraduodenal test meal. After the intraduodenal test meal in 4 normal subjects and in 17 DU patients an increase of gastric acid secretion and serum gastrin was measured. In basal state, after an intraduodenal or an oral test meal, DU patients with normal gastric acid secretory capacity had higher serum gastrin concentrations than DU patients with gastric hypersecretion. There was a good correlation between peak serum gastrin levels after the oral and after the intraduodenal test meal. From these data it is concluded: (1) Intraduodenal application of a test meal results in release of gastrin from extragastric sites. (2) Extragastric gastrin is biologically active. (3) DU patients are able to release more antral and more extragastric gastrin in response to a test meal. Further studies, however, are necessary to show the significance of these findings in the pathogenesis of peptic ulcer disease. | [
0.02098187431693077,
-0.02409663237631321,
0.04205188900232315,
0.009320538491010666,
0.04488508775830269,
-0.02194306068122387,
0.021334830671548843,
0.028935031965374947,
-0.0350005142390728,
-0.06499200314283371,
-0.0175329502671957,
-0.06653890758752823,
-0.001147259259596467,
0.006651996169239283,
0.04254026338458061,
-0.0254971981048584,
0.05069794878363609,
-0.05163034424185753,
-0.059670645743608475,
-0.003080088645219803,
-0.03724421188235283,
0.006338299717754126,
0.0580483004450798,
-0.024909095838665962,
0.010352158918976784,
0.02258886769413948,
-0.00862210988998413,
-0.04500306770205498,
-0.04639187827706337,
-0.1858789026737213,
0.07846570760011673,
-0.049658987671136856,
-0.010567626915872097,
-0.051242679357528687,
-0.020987821742892265,
-0.05761133134365082,
-0.036829568445682526,
-0.0004092040180694312,
0.0027501056902110577,
0.04247722029685974,
0.046140074729919434,
0.024584047496318817,
-0.014584152959287167,
-0.007154953200370073,
0.008750876411795616,
0.03401442617177963,
-0.04560443386435509,
0.018893660977482796,
0.1007075384259224,
0.07561589777469635,
0.033471062779426575,
0.022895880043506622,
0.026864787563681602,
0.07924669981002808,
0.05455431714653969,
-0.0209181047976017,
-0.013633888214826584,
-0.003428286872804165,
-0.05014651268720627,
0.007982735522091389,
0.007476494647562504,
0.047825440764427185,
-0.1245584711432457,
0.022681111469864845,
0.08034104108810425,
0.01386922225356102,
-0.04748915135860443,
-0.052357614040374756,
0.07098937034606934,
0.05583574250340462,
-0.050569966435432434,
0.03479531407356262,
0.02680368162691593,
0.07551437616348267,
-0.05108499526977539,
-0.026216937229037285,
0.0272509902715683,
-0.022884903475642204,
-0.00792287103831768,
0.01690049283206463,
0.0022996487095952034,
0.036228787153959274,
0.05356220155954361,
-0.0297684483230114,
0.029184261336922646,
-0.021628275513648987,
0.0442446731030941,
0.0023082345724105835,
-0.027727743610739708,
-0.02667766436934471,
-0.009418792091310024,
-0.03663665056228638,
-0.030405988916754723,
0.011254657059907913,
0.015406795777380466,
-0.056842487305402756,
0.015731604769825935,
-0.0660848394036293,
0.021992748603224754,
0.38388219475746155,
-0.05181191861629486,
-0.04991636052727699,
-0.028153523802757263,
-0.00006221203511813655,
0.0007569528534077108,
-0.00585176981985569,
0.03792908042669296,
-0.017451561987400055,
-0.05417194962501526,
0.04355523735284805,
0.030255138874053955,
0.1207939088344574,
0.004597912542521954,
-0.030860960483551025,
0.014017244800925255,
0.05800690874457359,
0.03976474329829216,
0.020963378250598907,
-0.0009397989488206804,
0.04873979464173317,
-0.03449823707342148,
0.06557847559452057,
-0.06194742023944855,
-0.08181234449148178,
0.004790291655808687,
0.008237294852733612,
0.021483248099684715,
0.028533820062875748,
-0.031442221254110336,
0.007173496764153242,
0.011319590732455254,
-0.06677255779504776,
-0.05621136724948883,
0.0182530228048563,
-0.015083393082022667,
-0.019843216985464096,
0.07593432068824768,
0.044812604784965515,
0.0010548620484769344,
-0.01919204741716385,
-0.049284450709819794,
-0.08753810822963715,
-0.0033296600449830294,
-0.09182169288396835,
-0.0017473463667556643,
0.10162245482206345,
-0.018629303202033043,
0.003996946383267641,
0.019614623859524727,
-0.04235284775495529,
0.03918717801570892,
0.045934248715639114,
-0.016042785719037056,
0.04512159898877144,
0.030734563246369362,
0.012331025674939156,
0.005027974024415016,
0.0162813663482666,
0.008719285018742085,
-0.02155010588467121,
-0.0071976990438997746,
-0.034149132668972015,
0.0025516559835523367,
-0.02206462435424328,
0.05995193496346474,
-0.027818690985441208,
-0.03700095787644386,
-0.05661575123667717,
0.029920507222414017,
-0.023927664384245872,
0.019765350967645645,
0.020933758467435837,
-0.07947369664907455,
-0.013837645761668682,
0.0375496931374073,
0.0007787703652866185,
-0.0018415359081700444,
-0.013719292357563972,
-0.0034097323659807444,
0.024234101176261902,
0.016775082796812057,
0.04180071875452995,
0.016801446676254272,
0.061558499932289124,
-0.015564612112939358,
-0.015214314684271812,
-0.02053966000676155,
0.03883105143904686,
0.025500420480966568,
0.07698377221822739,
-0.03423388674855232,
0.040621235966682434,
-0.1477314978837967,
-0.018297210335731506,
-0.04049217700958252,
-0.059042591601610184,
-0.006001411937177181,
0.01642877422273159,
-0.041838910430669785,
0.024915248155593872,
0.05149181932210922,
0.05529414489865303,
0.03752701357007027,
0.010373939760029316,
0.04504738375544548,
0.033710282295942307,
-0.004114674404263496,
-0.028099268674850464,
0.003681963076815009,
-0.03962640091776848,
-0.022524772211909294,
0.05236946418881416,
-0.008934441953897476,
0.03592570498585701,
-0.04757033288478851,
-0.008556431159377098,
0.0026405605021864176,
0.008970146998763084,
0.025818144902586937,
0.025402111932635307,
0.030259892344474792,
-0.012660463340580463,
0.006015070714056492,
-0.2837214767932892,
-0.04176534712314606,
0.03128081187605858,
-0.0247785747051239,
0.09662887454032898,
-0.04047754034399986,
0.00837383046746254,
-0.037701092660427094,
0.00866857822984457,
-0.02538464032113552,
0.03872082009911537,
0.06565814465284348,
-0.04468104615807533,
0.013609710149466991,
0.050120942294597626,
0.0673196092247963,
0.004844614304602146,
-0.044898103922605515,
0.05637863278388977,
0.0022868087980896235,
-0.044812340289354324,
-0.03468075394630432,
0.02532641589641571,
0.031960442662239075,
0.045131832361221313,
-0.038344256579875946,
0.12008780986070633,
0.007513164542615414,
-0.002168531296774745,
-0.05354728177189827,
-0.019048525020480156,
0.06834724545478821,
0.004160839132964611,
-0.04716639965772629,
0.02513766475021839,
-0.051747072488069534,
-0.0034111763816326857,
-0.037392012774944305,
-0.056182581931352615,
-0.04505942016839981,
-0.02770673856139183,
0.015007262118160725,
0.012588435783982277,
-0.06250818073749542,
-0.0011046051513403654,
-0.0149618498980999,
-0.02117001824080944,
0.06903193891048431,
-0.005756506696343422,
-0.007204959634691477,
0.04516630619764328,
0.004823111928999424,
0.01307686883956194,
0.012116678059101105,
-0.016228722408413887,
-0.032053787261247635,
-0.016588479280471802,
-0.004145669750869274,
-0.08020574599504471,
0.029937567189335823,
-0.018190855160355568,
0.023593386635184288,
0.004149413667619228,
0.014156532473862171,
0.039630286395549774,
0.023102296516299248,
-0.035451799631118774,
0.03745773434638977,
0.05225290730595589,
0.060865651816129684,
-0.0018490422517061234,
0.07378318905830383,
-0.010989135131239891,
-0.01673116348683834,
0.02624729461967945,
0.014909773133695126,
0.010899728164076805,
-0.05304819718003273,
-0.09292958676815033,
-0.012678321450948715,
0.045311324298381805,
-0.10548147559165955,
0.10262639820575714,
0.0041985418647527695,
-0.04102063924074173,
0.06003763526678085,
0.003470909083262086,
-0.0346396304666996,
0.021830672398209572,
0.0108967749401927,
-0.0019376636482775211,
0.029125168919563293,
-0.03731971234083176,
-0.04999867081642151,
-0.03300837054848671,
0.06629554182291031,
-0.29743674397468567,
0.04760713875293732,
-0.018702199682593346,
-0.017507528886198997,
0.010863165371119976,
0.05933934822678566,
-0.017336901277303696,
-0.02175285294651985,
-0.023865832015872,
0.027098273858428,
-0.0144762322306633,
-0.0009036777191795409,
0.06312061846256256,
-0.004787006415426731,
-0.048438720405101776,
-0.0029074663762003183,
0.0067693921737372875,
-0.03645574674010277,
-0.0021609480027109385,
0.006786824204027653,
0.01154092326760292,
-0.019462034106254578,
0.12392092496156693,
0.02468767575919628,
-0.026043584570288658,
0.02775067836046219,
0.021326150745153427,
0.04650549963116646,
0.01985660381615162,
0.013644772581756115,
0.03770425543189049,
0.012238864786922932,
-0.00850630272179842,
-0.004631353542208672,
0.0689164400100708,
0.06556475162506104,
0.015114950947463512,
-0.025016045197844505,
-0.000797213870100677,
-0.007522995118051767,
0.004508533049374819,
-0.06307672709226608,
-0.024418553337454796,
-0.05111293867230415,
0.0734747052192688,
-0.06961412727832794,
0.032178930938243866,
-0.09821044653654099,
0.016578707844018936,
-0.02540031261742115,
-0.04144781455397606,
-0.03841174393892288,
0.05159908905625343,
0.03794606775045395,
0.03501985967159271,
-0.049649160355329514,
-0.027463918551802635,
-0.09260004013776779,
-0.016915809363126755,
0.01599808968603611,
-0.004189123399555683,
-0.012640346772968769,
-0.028286896646022797,
0.04855644330382347,
-0.038404833525419235
] |
PMID:8357 | Effects of carbenoxolone on gastric mucosal permeability and blood flow in the dog. | The effects of topical application of carbenoxolone at neutral and acidic pH were compared in exteriorized, chambered segments of canine gastric corpus. When dissolved in saline at pH 7.5 to 8.0, 0.25% carbenoxolone caused a rapid drop in gastric potential difference of 56 +/- 2 mv and greatly increased permeability to H+ ions. Blood flow, as measured by radioactive microspheres, was not changed by carbenoxolone treatment, but subsequent exposure to isotonic HC1 caused an abrupt rise in flow. Application of 0.25% carbenoxolone suspension in isotonic HC1 caused no change in potential difference, permeability, or blood flow. Neither carbenoxolone preparation had a significant effect on aspirin-induced H+ back-diffusion or injury. | Effects of carbenoxolone on gastric mucosal permeability and blood flow in the dog. The effects of topical application of carbenoxolone at neutral and acidic pH were compared in exteriorized, chambered segments of canine gastric corpus. When dissolved in saline at pH 7.5 to 8.0, 0.25% carbenoxolone caused a rapid drop in gastric potential difference of 56 +/- 2 mv and greatly increased permeability to H+ ions. Blood flow, as measured by radioactive microspheres, was not changed by carbenoxolone treatment, but subsequent exposure to isotonic HC1 caused an abrupt rise in flow. Application of 0.25% carbenoxolone suspension in isotonic HC1 caused no change in potential difference, permeability, or blood flow. Neither carbenoxolone preparation had a significant effect on aspirin-induced H+ back-diffusion or injury. | [
-0.023236487060785294,
-0.021600091829895973,
0.0011498832609504461,
0.06092386320233345,
-0.026244493201375008,
-0.0011439635418355465,
0.03745805099606514,
0.03946983069181442,
-0.07312684506177902,
-0.04745570197701454,
0.01074888277798891,
-0.06051471456885338,
0.006204093806445599,
0.07512865215539932,
0.016436364501714706,
-0.025910701602697372,
0.048921968787908554,
-0.004183730576187372,
-0.07306665927171707,
-0.04434564709663391,
-0.10295592993497849,
-0.0008830961305648088,
0.02637476846575737,
-0.011538260616362095,
0.013720987364649773,
0.030785784125328064,
0.027188172563910484,
0.031443607062101364,
-0.06400205194950104,
-0.2092999368906021,
0.02221008762717247,
0.023463480174541473,
-0.011370993219316006,
-0.010960466228425503,
-0.03333510830998421,
0.027136795222759247,
0.010024528950452805,
0.019800549373030663,
0.020494723692536354,
0.06798061728477478,
0.02494002878665924,
0.03496316820383072,
-0.001558375544846058,
0.07833855599164963,
0.024282662197947502,
-0.010729211382567883,
-0.006876122206449509,
0.01991300657391548,
0.11303587257862091,
-0.006583109498023987,
0.056385401636362076,
-0.010383162647485733,
0.014882194809615612,
0.020511683076620102,
0.019433679059147835,
-0.05626339837908745,
-0.024951525032520294,
0.007474091369658709,
-0.01969441957771778,
0.04635104537010193,
0.017695985734462738,
0.05688483268022537,
-0.15252959728240967,
0.047879673540592194,
0.08285759389400482,
0.012593102641403675,
-0.04553278908133507,
-0.026962885633111,
0.0663716197013855,
0.07988351583480835,
-0.04633444547653198,
0.05498066172003746,
-0.04562823101878166,
0.022514084354043007,
0.07024987787008286,
-0.021039782091975212,
0.019357193261384964,
-0.024809658527374268,
-0.036498669534921646,
-0.011398001573979855,
-0.052638694643974304,
-0.010705246590077877,
-0.06430139392614365,
-0.07793733477592468,
-0.017707305029034615,
-0.03920227289199829,
0.07392482459545135,
-0.019450409337878227,
-0.02939603663980961,
0.01437933836132288,
-0.024319062009453773,
-0.05522414669394493,
-0.06215641275048256,
-0.0031672196928411722,
-0.016064394265413284,
0.004224082455039024,
-0.010645817033946514,
-0.04678774252533913,
0.03510827198624611,
0.337099552154541,
-0.040717922151088715,
0.02016316168010235,
-0.08487573266029358,
-0.010703605599701405,
0.05742412060499191,
0.013814223930239677,
0.03677314147353172,
0.0017196114640682936,
0.003753793193027377,
0.03984518349170685,
0.05748893693089485,
-0.02236383780837059,
0.04509029537439346,
-0.02504350244998932,
-0.0603380911052227,
0.055659037083387375,
-0.014172227121889591,
-0.017118539661169052,
0.01968885026872158,
0.023765385150909424,
-0.05490599945187569,
0.02353431098163128,
0.044176530092954636,
0.005747394170612097,
0.02914167009294033,
0.03961389884352684,
-0.001358230714686215,
0.04605528712272644,
-0.010259469971060753,
0.03652779012918472,
0.026715511456131935,
0.01358429528772831,
-0.015422243624925613,
0.02977423369884491,
-0.007925231009721756,
-0.03150282800197601,
0.008858555927872658,
0.06645988672971725,
0.012526488862931728,
0.012536359950900078,
-0.06587105244398117,
-0.04225483164191246,
-0.05361755192279816,
-0.08897219598293304,
-0.08002836257219315,
0.12545374035835266,
-0.018706005066633224,
0.012282066978514194,
-0.03958163782954216,
-0.030609212815761566,
-0.028394676744937897,
-0.017334042116999626,
0.0008920559193938971,
0.06803785264492035,
-0.051506537944078445,
-0.00006219617353053764,
-0.05335861071944237,
-0.029544807970523834,
-0.027037983760237694,
0.04162352904677391,
-0.01212533749639988,
-0.04971921816468239,
-0.03296465054154396,
0.07876674085855484,
0.02260204218327999,
0.009684007614850998,
-0.033280570060014725,
0.002778603695333004,
0.045683592557907104,
0.0654204785823822,
0.007110777776688337,
0.030678918585181236,
-0.0565444678068161,
-0.06785345822572708,
-0.04492158070206642,
0.021476926282048225,
0.041854940354824066,
-0.021483032032847404,
0.05308445915579796,
-0.0811312347650528,
0.07839176058769226,
0.08708897978067398,
0.019997835159301758,
-0.004065137822180986,
0.009933953173458576,
-0.03504268825054169,
-0.11191268265247345,
0.0438772551715374,
-0.02682431787252426,
0.05711490288376808,
-0.040299806743860245,
-0.02193947322666645,
-0.0183227751404047,
0.021750569343566895,
-0.04056064411997795,
0.014591882936656475,
-0.0825115367770195,
-0.051955077797174454,
-0.04741435870528221,
-0.010472679510712624,
0.05824095383286476,
0.017010800540447235,
0.006958711892366409,
-0.010953482240438461,
0.03932546079158783,
0.03728058561682701,
0.0077301799319684505,
-0.03254508599638939,
-0.000043752414057962596,
0.011548557318747044,
-0.023567838594317436,
0.0782465785741806,
0.006718688178807497,
0.044667135924100876,
0.01665760762989521,
0.057941582053899765,
-0.018248070031404495,
0.02942315861582756,
0.026029197499155998,
0.0038927197456359863,
0.07883011549711227,
-0.04152567684650421,
-0.026437334716320038,
-0.29735496640205383,
-0.021814825013279915,
-0.0269378200173378,
-0.05336267873644829,
0.08860950917005539,
-0.04197926074266434,
0.020834363996982574,
-0.04878297448158264,
-0.0034774329978972673,
-0.032433073967695236,
0.02432328090071678,
0.07551033049821854,
-0.04940218850970268,
0.0011116407113149762,
0.04178275913000107,
0.014586780220270157,
-0.0009489223011769354,
0.00033563192118890584,
0.053325217217206955,
-0.014344668947160244,
0.042363379150629044,
-0.022691169753670692,
-0.03099236451089382,
-0.03277696296572685,
-0.009531793184578419,
0.0003238798235543072,
0.10194892436265945,
-0.050431132316589355,
-0.03585926070809364,
-0.05719103291630745,
-0.0036060232669115067,
0.008603228256106377,
-0.008431714959442616,
-0.10930042713880539,
-0.011412057094275951,
-0.01871192455291748,
-0.022038916125893593,
0.023122869431972504,
-0.03950324282050133,
-0.03225874528288841,
-0.04584396257996559,
0.02426820620894432,
-0.007387889549136162,
-0.0409720316529274,
-0.020925557240843773,
0.0026535033248364925,
0.03105545975267887,
0.05140049755573273,
-0.03995192423462868,
-0.006873397622257471,
0.04059586301445961,
0.014429793693125248,
-0.04709978774189949,
0.0070282286033034325,
0.045357801020145416,
-0.03366450220346451,
0.018994208425283432,
0.0294241514056921,
-0.07455150038003922,
0.0067581189796328545,
0.021637536585330963,
-0.0366939976811409,
0.08522052317857742,
-0.02910640463232994,
0.00827794335782528,
0.04394492134451866,
0.007568161468952894,
0.0006893100799061358,
0.05113526061177254,
0.047877583652734756,
0.012603064067661762,
0.15158042311668396,
-0.04532785713672638,
0.0027345018461346626,
0.06342756748199463,
-0.000784300034865737,
0.011154698207974434,
-0.026040861383080482,
-0.06757161021232605,
-0.03318865969777107,
0.00042780814692378044,
-0.07167112827301025,
0.06777241080999374,
0.0087468596175313,
0.012814651243388653,
0.041338153183460236,
-0.004250871948897839,
-0.09466207027435303,
0.030129753053188324,
0.0015779307577759027,
0.03271540254354477,
-0.011036735028028488,
-0.038220860064029694,
-0.04760652035474777,
0.008569180965423584,
0.01979975774884224,
-0.2213934361934662,
0.001559784752316773,
0.043272465467453,
0.004045427776873112,
-0.011620290577411652,
-0.005233366973698139,
0.026520613580942154,
-0.07152638584375381,
-0.004187966231256723,
-0.01512826420366764,
-0.02391352690756321,
0.040071338415145874,
0.04947177320718765,
0.02501598745584488,
-0.059672459959983826,
0.004407484550029039,
0.06236892193555832,
0.010919669643044472,
0.007378190290182829,
0.061564505100250244,
0.022766541689634323,
-0.0182881448417902,
0.15685877203941345,
0.009360692463815212,
-0.02977938950061798,
-0.005381764378398657,
-0.019065670669078827,
0.028176337480545044,
0.04875212907791138,
0.028276480734348297,
0.004810208920389414,
0.03994826599955559,
0.0783376693725586,
-0.009944412857294083,
0.07198674976825714,
-0.03384791314601898,
-0.07851967960596085,
-0.004712854977697134,
-0.04729880392551422,
0.027388932183384895,
0.02958463877439499,
0.017749382182955742,
-0.0055735185742378235,
-0.06091509386897087,
0.006094356067478657,
-0.02440708875656128,
0.028810445219278336,
-0.06357086449861526,
0.012467063963413239,
-0.011150175705552101,
-0.018124356865882874,
-0.05121484398841858,
0.059416987001895905,
0.02539733611047268,
0.0502762496471405,
-0.004374173004180193,
0.016568271443247795,
-0.024637866765260696,
-0.006874897051602602,
-0.03258867934346199,
0.05133648216724396,
-0.03279396891593933,
-0.02037280984222889,
0.04840923473238945,
0.030570274218916893
] |
PMID:8360 | Recombinant clone heterogeneity in Escherichia coli conjunction: effect of pH and partially replicated recipient deoxyribonucleic acid. | At pH 6.8, a substantial fraction of recombinant colonies obtained from conjugation with an HfrH donor contained multiple recombinant classes in a single colony (polygenotype colony). In contrast, when the conjugation was performed at pH 7.6, the number of polygenotypic colonies was drastically reduced, and the recombinant colonies were predominantly monogenotypic or digenotypic. Genetic analysis revealed that the digenotypic recombinants differ in those donor markers near the origin of DNA replication but share those donor markers near the terminus. This integration pattern suggests that the formation of digenotypic recombinants involves recombination of a single copy of the exogenome with a partially replicated recipient DNA molecule. This suggestion was supported by examination of the genotype of recombinant colonies recovered from crosses with an HfrKL96 donor which was derived from HfrH but transfers its chromosome in the reverse direction. | Recombinant clone heterogeneity in Escherichia coli conjunction: effect of pH and partially replicated recipient deoxyribonucleic acid. At pH 6.8, a substantial fraction of recombinant colonies obtained from conjugation with an HfrH donor contained multiple recombinant classes in a single colony (polygenotype colony). In contrast, when the conjugation was performed at pH 7.6, the number of polygenotypic colonies was drastically reduced, and the recombinant colonies were predominantly monogenotypic or digenotypic. Genetic analysis revealed that the digenotypic recombinants differ in those donor markers near the origin of DNA replication but share those donor markers near the terminus. This integration pattern suggests that the formation of digenotypic recombinants involves recombination of a single copy of the exogenome with a partially replicated recipient DNA molecule. This suggestion was supported by examination of the genotype of recombinant colonies recovered from crosses with an HfrKL96 donor which was derived from HfrH but transfers its chromosome in the reverse direction. | [
-0.036735985428094864,
-0.026085415855050087,
-0.021314939484000206,
-0.04194916412234306,
-0.008125127293169498,
0.00035103404661640525,
0.014027786441147327,
0.046055275946855545,
0.00463998643681407,
0.02189548872411251,
0.03803502023220062,
-0.08212187886238098,
0.04821831360459328,
-0.04707442969083786,
-0.042918749153614044,
-0.007132955361157656,
-0.10507891327142715,
-0.032825712114572525,
-0.04476426914334297,
-0.051791220903396606,
0.01035957969725132,
-0.05792059376835823,
0.0042101251892745495,
0.01523399818688631,
0.017878277227282524,
-0.014084279537200928,
0.017518799751996994,
-0.00775877246633172,
-0.09365683048963547,
-0.2333977222442627,
-0.014930502511560917,
0.022055823355913162,
0.010615327395498753,
-0.012482251971960068,
-0.002079880563542247,
0.012936132960021496,
-0.009267975576221943,
0.0011571660870686173,
-0.06543012708425522,
0.03515598550438881,
-0.023105628788471222,
0.001379521214403212,
0.042280927300453186,
0.04402976110577583,
0.028593312948942184,
-0.0382084883749485,
-0.05987560376524925,
0.017616206780076027,
0.026104936376214027,
0.03016154281795025,
0.017473096027970314,
-0.037732236087322235,
0.00378425233066082,
0.06834769248962402,
0.0006338359671644866,
0.06498753279447556,
0.029412424191832542,
0.03130536526441574,
0.010834557004272938,
-0.019907444715499878,
0.021188518032431602,
0.037734925746917725,
-0.12847328186035156,
0.06652987748384476,
0.08327103406190872,
0.00834411196410656,
-0.022584358230233192,
-0.01676916889846325,
0.021265624091029167,
0.05307330563664436,
-0.04993681609630585,
0.008833234198391438,
-0.026640143245458603,
-0.03402988240122795,
0.02249918319284916,
0.008997240103781223,
-0.011991210281848907,
-0.014563454315066338,
0.04135170206427574,
0.014966649934649467,
-0.026268871501088142,
-0.03227120265364647,
-0.012875446118414402,
-0.03107490949332714,
-0.003125221934169531,
-0.007181875873357058,
-0.0014811106957495213,
-0.0426090843975544,
-0.006305209826678038,
0.04604533687233925,
0.042793743312358856,
-0.008449331857264042,
-0.001469918992370367,
-0.018938899040222168,
-0.056840721517801285,
0.042191244661808014,
0.04387316852807999,
-0.05434459075331688,
0.11956804245710373,
0.34726765751838684,
-0.03844401612877846,
0.004919859115034342,
-0.024936862289905548,
0.021480854600667953,
-0.011567163281142712,
-0.0038101295940577984,
-0.052435167133808136,
-0.0005822927341796458,
0.007084264885634184,
0.030363252386450768,
-0.06584572792053223,
0.011474219150841236,
0.06295119971036911,
-0.042727045714855194,
-0.05727608874440193,
-0.0047098249197006226,
-0.019226161763072014,
0.015443913638591766,
-0.01614469103515148,
-0.005576686467975378,
0.004240084905177355,
0.007033502217382193,
-0.029350968077778816,
-0.02500961907207966,
0.025417229160666466,
0.03533129021525383,
-0.030957933515310287,
0.06907757371664047,
0.03414086997509003,
-0.011894379742443562,
0.017488043755292892,
0.026053903624415398,
0.017737314105033875,
-0.017825104296207428,
-0.012277339585125446,
0.02990732714533806,
-0.010793487541377544,
-0.049032073467969894,
0.005158671643584967,
-0.04700331389904022,
-0.028810998424887657,
-0.03342556580901146,
-0.026446985080838203,
-0.03070022352039814,
-0.08407970517873764,
0.08769746869802475,
-0.030765701085329056,
0.09509294480085373,
-0.026664134114980698,
0.0095966886729002,
-0.013812975957989693,
0.0959935262799263,
0.019985632970929146,
0.007613065652549267,
-0.0007185279973782599,
0.08317107707262039,
-0.012779101729393005,
-0.012390708550810814,
0.02561967633664608,
0.007988279685378075,
-0.006427338346838951,
0.014388706535100937,
0.040029991418123245,
0.08683180063962936,
0.03847253695130348,
-0.05222095176577568,
-0.00855329167097807,
0.036090001463890076,
0.026345575228333473,
0.0028458337765187025,
-0.039279479533433914,
0.015219969674944878,
0.008605562150478363,
-0.02064663916826248,
-0.03603581339120865,
-0.03500095382332802,
-0.07294037938117981,
0.016469206660985947,
0.04587128758430481,
0.007054982241243124,
0.03584233298897743,
-0.0306320171803236,
-0.03401870280504227,
0.017417514696717262,
0.04015832394361496,
-0.06927567720413208,
-0.08361440151929855,
0.0020286499056965113,
-0.05011913552880287,
0.05575777590274811,
-0.030679874122142792,
-0.02318771742284298,
-0.040804293006658554,
0.0447421595454216,
0.0635831281542778,
-0.01239046361297369,
-0.05770474672317505,
0.010141645558178425,
0.008777246810495853,
0.011157136410474777,
0.02410486340522766,
0.07927050441503525,
0.014975144527852535,
0.011176629923284054,
-0.01603783294558525,
0.022482795640826225,
0.10801281780004501,
-0.02258264273405075,
0.05330367758870125,
0.0070274448953568935,
-0.07737866789102554,
0.018182380124926567,
-0.03594302758574486,
-0.02087191864848137,
-0.05824132636189461,
0.07104410976171494,
0.050280459225177765,
0.016159752383828163,
0.11604665964841843,
0.05289706960320473,
-0.0011711583938449621,
-0.05889567732810974,
0.006703582126647234,
-0.3119684159755707,
-0.05208313837647438,
-0.015829166397452354,
0.03481033071875572,
0.006792047061026096,
-0.0019607641734182835,
0.004188242368400097,
0.0473063588142395,
0.01471348199993372,
-0.04633861407637596,
0.0593661367893219,
0.0833151713013649,
-0.05804460123181343,
-0.02715396136045456,
0.009638591669499874,
0.027314022183418274,
0.007899753749370575,
0.010049906559288502,
-0.014678053557872772,
0.0038656468968838453,
0.007091242354363203,
0.018518488854169846,
0.040131259709596634,
0.01715523563325405,
0.03937413543462753,
0.0060997274704277515,
0.11911427229642868,
-0.020990872755646706,
0.020568521693348885,
0.005955880042165518,
0.02459803968667984,
0.04894757643342018,
-0.0008290404221042991,
0.008798961527645588,
0.008729913271963596,
0.004354547709226608,
0.0046505071222782135,
-0.03458728268742561,
0.039079323410987854,
0.007029150612652302,
0.021823914721608162,
-0.04071316868066788,
-0.05969718098640442,
-0.06683073192834854,
0.007890358567237854,
-0.0341142937541008,
-0.0434335321187973,
0.019720114767551422,
-0.0062738629058003426,
0.050702475011348724,
0.023849545046687126,
-0.01582960970699787,
0.011729342862963676,
0.007545072119683027,
0.008648591116070747,
0.0602559894323349,
-0.020527081564068794,
-0.006376579403877258,
-0.021352866664528847,
-0.033668894320726395,
0.027926970273256302,
0.04560140147805214,
0.0014263114426285028,
-0.009651491418480873,
0.007594204042106867,
-0.04995109513401985,
0.007393889129161835,
0.027925614267587662,
0.06243520602583885,
-0.010189740918576717,
0.04793165251612663,
0.12289182841777802,
-0.011383522301912308,
0.03504610061645508,
-0.02469598315656185,
0.0018650471465662122,
-0.02891797013580799,
-0.06468327343463898,
-0.06435945630073547,
-0.013227871619164944,
0.017559455707669258,
-0.047421954572200775,
0.04202469810843468,
0.0013071143766865134,
0.029495881870388985,
0.02906808815896511,
0.03495171666145325,
0.022018171846866608,
0.058729659765958786,
0.08126617223024368,
-0.024206070229411125,
0.01783188432455063,
0.012106191366910934,
-0.022085435688495636,
-0.04775253310799599,
-0.015209703706204891,
-0.2829911708831787,
0.03411188721656799,
0.01313094887882471,
0.08242559432983398,
-0.03532152250409126,
0.020530493929982185,
0.0668344795703888,
-0.055534616112709045,
0.005444406531751156,
0.04185980185866356,
0.01876743696630001,
-0.03837694227695465,
0.06543110311031342,
0.01535613089799881,
-0.028611307963728905,
0.0247722826898098,
0.04082932323217392,
-0.07833712548017502,
-0.010835858061909676,
-0.07525386661291122,
-0.011845407076179981,
-0.057239145040512085,
0.17386090755462646,
0.0024620050098747015,
0.04425489529967308,
-0.009867476299405098,
0.0014743467327207327,
0.02795938402414322,
-0.046239979565143585,
-0.008322237990796566,
-0.056267715990543365,
0.052950382232666016,
0.06235649809241295,
-0.057172223925590515,
0.024815447628498077,
-0.009662657044827938,
-0.007648138795047998,
-0.034053850919008255,
-0.007782652508467436,
0.029571937397122383,
-0.0298997163772583,
0.001173330587334931,
-0.09383918344974518,
-0.027738019824028015,
0.03761769458651543,
-0.017533378675580025,
-0.03224927559494972,
-0.08596570789813995,
-0.05711222440004349,
0.011726999655365944,
-0.07297089695930481,
0.015548629686236382,
0.025477765128016472,
-0.03377041593194008,
-0.0018138490850105882,
-0.0088725034147501,
-0.032528799027204514,
-0.02082257717847824,
0.02389393001794815,
0.017123816534876823,
0.07218107581138611,
0.005287193227559328,
-0.05590585991740227,
0.0803469642996788,
-0.012057330459356308
] |
PMID:8361 | [Galenic studies of a new gastrotherapeutic drug]. | This paper presents the methods for galenical investigations of a new gastrotherapeutic preparation. The results and their possible relations to therapy are discussed. The preparation is presented a press-coated tablet. Its antacid characteristics concerning the rate of effectiveness, potency and duration are compared with leading brands on the German market. Pepsin inactivation together with the rapid liberation of the spasmolytic agent propantheline and the psychically stabilising agent peranzine are demonstrated. The results of the stability tests for the active ingredients and the in vitro availability are described. The new product is a gastrotherapeutic preparation, which fulfils all requirements for rapid action and reliable therapy. | [Galenic studies of a new gastrotherapeutic drug]. This paper presents the methods for galenical investigations of a new gastrotherapeutic preparation. The results and their possible relations to therapy are discussed. The preparation is presented a press-coated tablet. Its antacid characteristics concerning the rate of effectiveness, potency and duration are compared with leading brands on the German market. Pepsin inactivation together with the rapid liberation of the spasmolytic agent propantheline and the psychically stabilising agent peranzine are demonstrated. The results of the stability tests for the active ingredients and the in vitro availability are described. The new product is a gastrotherapeutic preparation, which fulfils all requirements for rapid action and reliable therapy. | [
-0.025378532707691193,
0.058925434947013855,
-0.027004575356841087,
0.011340713128447533,
0.0417083278298378,
0.001238822122104466,
0.046250518411397934,
0.058787308633327484,
-0.04122355952858925,
-0.1090017706155777,
-0.02128509059548378,
-0.019146665930747986,
-0.013283860869705677,
0.046874336898326874,
-0.020850887522101402,
-0.0033799849916249514,
0.0746828094124794,
-0.04141213372349739,
-0.038186557590961456,
-0.004563819617033005,
-0.009355173446238041,
-0.02926671877503395,
-0.036864642053842545,
-0.04921518638730049,
0.010369532741606236,
0.061447013169527054,
-0.010883529670536518,
0.021749090403318405,
-0.06716926395893097,
-0.17542549967765808,
0.0026099381502717733,
-0.09128657728433609,
-0.02347799576818943,
-0.03057240881025791,
-0.05862194299697876,
0.042377769947052,
-0.030015669763088226,
0.0486699640750885,
-0.03102818690240383,
0.03505394980311394,
0.01940939947962761,
0.015917720273137093,
-0.0422389842569828,
0.02893454022705555,
0.030884629115462303,
0.020870391279459,
-0.014614184387028217,
-0.011757245287299156,
0.06357351690530777,
0.013667185790836811,
-0.0072846971452236176,
-0.0005776063189841807,
0.04731941595673561,
0.0011366931721568108,
-0.01860763505101204,
0.02013588137924671,
-0.04246414080262184,
-0.003910431172698736,
-0.0240683164447546,
0.025388363748788834,
0.041041746735572815,
-0.02263569086790085,
-0.1805521547794342,
0.02799791842699051,
0.03994544595479965,
0.03533042222261429,
-0.0029564944561570883,
-0.06391610205173492,
0.07640450447797775,
0.09242124110460281,
-0.033086083829402924,
0.002694555325433612,
-0.003375406377017498,
0.057134781032800674,
-0.03569483757019043,
-0.01171303354203701,
0.007775674108415842,
-0.001748960348777473,
-0.05939032882452011,
0.0318574383854866,
0.04844239354133606,
0.023975566029548645,
0.033939238637685776,
-0.05178888887166977,
0.020349131897091866,
-0.0441628098487854,
0.023204606026411057,
0.018346408382058144,
0.027673615142703056,
0.0031072760466486216,
0.0334494523704052,
-0.015760118141770363,
-0.03418485075235367,
-0.0724133625626564,
-0.0308392234146595,
-0.05972902476787567,
-0.047390252351760864,
-0.01522149983793497,
0.010042681358754635,
0.3882865607738495,
0.004039250314235687,
0.006404795218259096,
0.029092924669384956,
-0.03766831383109093,
0.006245088297873735,
-0.006648295558989048,
0.005050951149314642,
0.010646924376487732,
-0.0027268133126199245,
0.014872591011226177,
0.055053677409887314,
0.03234034404158592,
0.04746149852871895,
-0.05476151406764984,
0.00539043452590704,
0.05587377771735191,
0.024107256904244423,
-0.040918752551078796,
-0.04582087695598602,
0.00601861672475934,
-0.045917246490716934,
0.00990182813256979,
0.022095870226621628,
-0.021226072683930397,
0.03191673383116722,
-0.014266402460634708,
-0.08883010596036911,
0.0659860223531723,
-0.03249679133296013,
0.0324997641146183,
0.06587930023670197,
0.032028742134571075,
-0.003612345550209284,
0.04303987696766853,
0.0389786995947361,
-0.01403247844427824,
0.023670455440878868,
-0.004244470037519932,
0.030019709840416908,
-0.015192939899861813,
-0.03866316005587578,
-0.008294370025396347,
-0.028411034494638443,
-0.09872984886169434,
-0.09256210178136826,
0.039587970823049545,
0.04638684540987015,
0.0063239154405891895,
-0.012874836102128029,
0.03185479715466499,
-0.036697760224342346,
-0.0005592731758952141,
0.00984401348978281,
0.07315684109926224,
-0.027976561337709427,
0.0239852387458086,
0.0034655360504984856,
0.004041429143399,
-0.05536800995469093,
0.020827144384384155,
-0.01057843305170536,
-0.05020981281995773,
-0.019665362313389778,
0.06176077947020531,
-0.04125309735536575,
-0.06468427926301956,
0.004086386878043413,
-0.01400424912571907,
0.02946154959499836,
0.0207156203687191,
0.0006856233230791986,
0.0030523170717060566,
-0.00712544796988368,
0.05609723553061485,
-0.050765786319971085,
-0.01082235760986805,
0.00513441814109683,
-0.0369049534201622,
0.04139654338359833,
-0.01219130214303732,
0.028633201494812965,
0.04943093657493591,
0.025557029992341995,
0.04516950994729996,
-0.054644547402858734,
-0.024395283311605453,
-0.005380709655582905,
-0.007893296889960766,
0.02961474098265171,
0.06833957880735397,
-0.057186372578144073,
-0.014406101778149605,
-0.08541106432676315,
0.02611701376736164,
-0.004513503052294254,
0.0010164657142013311,
-0.059165023267269135,
0.026943130418658257,
-0.023251892998814583,
0.012237857095897198,
0.008742463774979115,
0.020211372524499893,
0.030447572469711304,
0.06498569995164871,
0.13358239829540253,
0.031626567244529724,
0.03189461678266525,
-0.006892419885843992,
0.014070557430386543,
-0.050985898822546005,
-0.0025302772410213947,
0.10825986415147781,
-0.024696066975593567,
0.019335493445396423,
-0.05816507339477539,
0.04244479537010193,
-0.02643175609409809,
-0.020010827109217644,
-0.008283102884888649,
0.05625704303383827,
0.012554056942462921,
-0.027737729251384735,
-0.02194397710263729,
-0.29262977838516235,
-0.044087957590818405,
-0.008540738373994827,
-0.002789484802633524,
0.0957777127623558,
-0.060982123017311096,
0.04428613558411598,
-0.023891104385256767,
-0.008730201981961727,
-0.06352098286151886,
0.02763221599161625,
0.09332935512065887,
-0.022554554045200348,
0.02239048480987549,
0.02298590913414955,
0.06341943144798279,
0.025178728625178337,
-0.028585925698280334,
0.0579235702753067,
-0.04493504762649536,
-0.005285660736262798,
-0.006284872069954872,
0.02837357483804226,
-0.004656644072383642,
0.07748080790042877,
-0.01160876452922821,
0.12146750092506409,
0.029824718832969666,
-0.054843720048666,
-0.02561701089143753,
0.035689909011125565,
0.025246581062674522,
-0.07497536391019821,
-0.08592748641967773,
0.024521468207240105,
-0.058640219271183014,
0.012021002359688282,
0.0010540660005062819,
-0.026427632197737694,
-0.01397948432713747,
-0.0024529024958610535,
-0.014698715880513191,
0.04976269602775574,
-0.048694908618927,
-0.02687489241361618,
0.02214588224887848,
-0.03126950189471245,
0.043261539191007614,
-0.04289072006940842,
0.0216847974807024,
0.02583102509379387,
0.038069773465394974,
-0.015474678948521614,
-0.009017115458846092,
-0.014434944838285446,
-0.0266652163118124,
-0.002117455005645752,
0.01927233673632145,
-0.07677873224020004,
-0.033266279846429825,
-0.025599638000130653,
-0.02903583273291588,
0.010993003845214844,
0.012081485241651535,
-0.013523448258638382,
-0.0028239083476364613,
-0.0011601634323596954,
0.0010164461564272642,
0.0542282834649086,
0.022261664271354675,
0.01579383574426174,
0.07306875288486481,
-0.040903929620981216,
0.03566562384366989,
0.058725178241729736,
-0.01932084932923317,
0.039835505187511444,
0.00761815719306469,
-0.046800319105386734,
0.07113766670227051,
-0.021132519468665123,
0.051975566893815994,
0.042294152081012726,
0.03338738903403282,
-0.029301883652806282,
-0.008590279147028923,
-0.012337304651737213,
-0.04391017556190491,
0.056082833558321,
0.01217519212514162,
0.023486541584134102,
0.04474269971251488,
-0.0458311028778553,
-0.04617537558078766,
0.03172948956489563,
0.03760693594813347,
-0.2798340916633606,
0.0314040407538414,
0.005697640124708414,
0.027522888034582138,
0.003960337024182081,
-0.021998438984155655,
0.020254362374544144,
-0.014883890748023987,
-0.02523150108754635,
-0.03088962472975254,
-0.016822483390569687,
-0.01597713865339756,
0.03606044128537178,
-0.004586202558130026,
-0.03675895556807518,
-0.03704707324504852,
0.05263949930667877,
-0.03649201616644859,
0.011306888423860073,
-0.035835977643728256,
0.0697752833366394,
-0.04268190264701843,
0.1652543842792511,
0.02529204450547695,
-0.08001852035522461,
0.05192415416240692,
0.0535711944103241,
0.04680413752794266,
-0.013621215708553791,
0.020531700924038887,
-0.025163091719150543,
-0.0055040232837200165,
0.009994089603424072,
-0.04379024729132652,
0.04388364776968956,
0.04902253672480583,
-0.05485145375132561,
-0.0033052759245038033,
-0.02014990895986557,
-0.04421904310584068,
-0.014464701525866985,
-0.009207331575453281,
-0.016385206952691078,
-0.003886073362082243,
0.09689956903457642,
-0.08577144145965576,
0.02654893510043621,
-0.08497500419616699,
-0.05540706589818001,
-0.016107819974422455,
-0.04265522584319115,
-0.03145717456936836,
0.0198065172880888,
0.0026370726991444826,
0.017694680020213127,
-0.046340461820364,
0.029834166169166565,
-0.013500926084816456,
0.02602020464837551,
0.013613685965538025,
0.019960511475801468,
-0.0030066506005823612,
0.025009918957948685,
0.07399194687604904,
0.03536267578601837
] |
PMID:8362 | [Field study on the decrease of lipids using etofibrate]. | A field-trial on Etofibrat was performed on 4405 patients suffering from primary and secondary hyperlipoproteinemia. The results were proved statistically. After a 3 to 4 weeks treatment the concentration of cholesterol as well as tryglicerides in the serum decreased significantly. After 6 to 8 weeks treatment the lipid-lowering effect was even stronger. Nearly 90% of the patients investigated gave a positive response to the treatment. Part of the population had undergone a treatment with other lipid-lowering agents before-most likely without sufficient success-in these cases a further lipid-lowering effect due to Etofibrat could be shown. Under-dosage in premedication cannot be excluded. The stratification of the patients in different groups of diagnosis showed a nearly similarity of both blood lipids independent of the diagnosis. This could also be confirmed for the group of patients suffering from diabetes. To prove the lipid-lowering efficacy of Etofibrat a population was withdrawn from treatment. Cholesterol as well as tryglicerides increased significantly during the interval without treatment. During a long-term study both lipid fractions could be kept down without increasing of the daily Etofibrat dose. The tolerance of Etofibrat was stated to be good up to very good. Objectively the measured enzymes SGOT, SGPT and gamma-GT showed a decrease of the means. Subjectively the occurrence of an often intermediate heat sensation and/or rubedo was of relevance. The low daily dose compared with other lipid-lowering agents gives indication for a better pharmacocinetic behaviour of the drug; | [Field study on the decrease of lipids using etofibrate]. A field-trial on Etofibrat was performed on 4405 patients suffering from primary and secondary hyperlipoproteinemia. The results were proved statistically. After a 3 to 4 weeks treatment the concentration of cholesterol as well as tryglicerides in the serum decreased significantly. After 6 to 8 weeks treatment the lipid-lowering effect was even stronger. Nearly 90% of the patients investigated gave a positive response to the treatment. Part of the population had undergone a treatment with other lipid-lowering agents before-most likely without sufficient success-in these cases a further lipid-lowering effect due to Etofibrat could be shown. Under-dosage in premedication cannot be excluded. The stratification of the patients in different groups of diagnosis showed a nearly similarity of both blood lipids independent of the diagnosis. This could also be confirmed for the group of patients suffering from diabetes. To prove the lipid-lowering efficacy of Etofibrat a population was withdrawn from treatment. Cholesterol as well as tryglicerides increased significantly during the interval without treatment. During a long-term study both lipid fractions could be kept down without increasing of the daily Etofibrat dose. The tolerance of Etofibrat was stated to be good up to very good. Objectively the measured enzymes SGOT, SGPT and gamma-GT showed a decrease of the means. Subjectively the occurrence of an often intermediate heat sensation and/or rubedo was of relevance. The low daily dose compared with other lipid-lowering agents gives indication for a better pharmacocinetic behaviour of the drug; | [
0.013558751903474331,
0.030188104137778282,
0.02242138236761093,
0.06178375333547592,
0.0009230802534148097,
-0.007027867715805769,
-0.00007215471850940958,
0.030706657096743584,
-0.07180913537740707,
-0.030128153041005135,
0.018786126747727394,
-0.022577490657567978,
0.04246704280376434,
-0.013013784773647785,
-0.03503546863794327,
-0.015955721959471703,
0.011963252909481525,
-0.024571077898144722,
-0.03998979553580284,
0.016987135633826256,
-0.005883536767214537,
-0.01797851361334324,
0.043789539486169815,
0.01405858714133501,
0.036636076867580414,
0.025145383551716805,
-0.030249813571572304,
-0.06356066465377808,
-0.03376103565096855,
-0.16322630643844604,
0.058347269892692566,
-0.010356209240853786,
0.04241511970758438,
-0.0202627032995224,
-0.03229973837733269,
0.04274097830057144,
-0.03321269899606705,
0.03414115309715271,
-0.08271238952875137,
0.06005151942372322,
0.021336164325475693,
0.005861755460500717,
-0.030249852687120438,
0.05356074124574661,
0.00787220150232315,
0.010509360581636429,
-0.025200385600328445,
0.00782462302595377,
0.05592890456318855,
0.05495661869645119,
-0.017946556210517883,
-0.03381023928523064,
0.018205976113677025,
0.06415224820375443,
-0.016644513234496117,
-0.028306173160672188,
-0.015164149925112724,
0.06112176179885864,
-0.018699204549193382,
0.03663618862628937,
-0.024865295737981796,
0.0262234378606081,
-0.13642087578773499,
0.0732303112745285,
-0.0030335928313434124,
0.01693897135555744,
-0.005720872897654772,
-0.017626726999878883,
0.014889130368828773,
0.0849306508898735,
-0.023074127733707428,
0.0620441734790802,
-0.028405940160155296,
-0.01955503225326538,
-0.01763090305030346,
-0.010671811178326607,
0.0012840682175010443,
-0.031148964539170265,
-0.017643842846155167,
0.02917981520295143,
0.010967439040541649,
-0.0172564834356308,
-0.004077346529811621,
-0.003455369733273983,
-0.02161877602338791,
-0.009211346507072449,
0.024628564715385437,
-0.03535640984773636,
0.004033305216580629,
-0.007998346351087093,
0.0722426027059555,
-0.0017518317326903343,
0.0028775136452168226,
-0.015613009221851826,
-0.06726673990488052,
-0.002488949103280902,
-0.005520846229046583,
-0.014376703649759293,
0.013426704332232475,
0.4018722176551819,
-0.016088269650936127,
0.020611263811588287,
-0.02799682691693306,
0.005128477234393358,
0.011799909174442291,
-0.04043401777744293,
-0.014642208814620972,
-0.03040357306599617,
0.05835388973355293,
0.051456790417432785,
0.0142903383821249,
-0.03215871378779411,
0.048984963446855545,
-0.031017987057566643,
-0.010810501873493195,
0.027424603700637817,
0.003950373735278845,
0.0015207375399768353,
-0.05496715009212494,
0.06007087230682373,
-0.09514409303665161,
0.04634522274136543,
0.04281638562679291,
0.023185603320598602,
0.06290508806705475,
0.033865898847579956,
-0.019474156200885773,
0.0749351903796196,
0.07180078327655792,
0.015632733702659607,
0.03758149966597557,
-0.02673276886343956,
-0.06434260308742523,
-0.04693113639950752,
-0.01786230318248272,
0.0135832903906703,
0.03761943057179451,
0.023947279900312424,
0.012576519511640072,
0.01356269046664238,
0.017896408215165138,
-0.038353025913238525,
-0.05509449169039726,
-0.04188743978738785,
0.025994615629315376,
0.08000562340021133,
0.05798184126615524,
0.06577716767787933,
-0.05877775326371193,
-0.027841724455356598,
0.043924544006586075,
-0.01895539090037346,
0.007548270747065544,
0.057832274585962296,
-0.013208280317485332,
0.04661906138062477,
0.013600978069007397,
0.03160075098276138,
-0.052297886461019516,
-0.017755750566720963,
-0.09697217494249344,
-0.05907243862748146,
-0.05106726661324501,
0.05826668068766594,
-0.02952692098915577,
-0.03992687165737152,
-0.07495184242725372,
0.005247834604233503,
-0.026577632874250412,
-0.0006769689498469234,
0.008607241325080395,
-0.0213815588504076,
0.015049894340336323,
-0.05983171612024307,
-0.03620923310518265,
-0.02859080582857132,
-0.0052479831501841545,
-0.05071486532688141,
0.03405420109629631,
-0.00883947592228651,
0.05956881120800972,
0.05120333656668663,
-0.013284629210829735,
-0.014505022205412388,
-0.01768997125327587,
-0.014854345470666885,
-0.032312534749507904,
0.047730397433042526,
0.017107577994465828,
-0.004832228180021048,
-0.0753430426120758,
-0.005094053689390421,
-0.07575348019599915,
0.042434658855199814,
-0.016408415511250496,
-0.06843125075101852,
-0.08337269723415375,
0.03442240133881569,
-0.0048921783454716206,
0.014344273135066032,
0.07793053984642029,
0.034245386719703674,
0.05401381477713585,
0.06997020542621613,
0.0925946980714798,
0.05422923341393471,
0.002789074322208762,
-0.0547354519367218,
0.07643099874258041,
-0.032282352447509766,
0.017031244933605194,
0.034834083169698715,
-0.004549700766801834,
0.05821383371949196,
-0.03265013173222542,
0.05521173030138016,
0.01929864101111889,
-0.01265992596745491,
-0.0224319938570261,
0.010571152903139591,
-0.002763930941000581,
0.0031638231594115496,
-0.004609177820384502,
-0.27659013867378235,
-0.04177805408835411,
0.012218735180795193,
-0.020958395674824715,
0.024083497002720833,
-0.050198379904031754,
-0.05582476779818535,
-0.04404902830719948,
0.008391234092414379,
-0.02048731967806816,
0.028616197407245636,
0.01674390584230423,
-0.021535690873861313,
-0.026873284950852394,
-0.02757425606250763,
-0.013005854561924934,
0.03613891452550888,
-0.03860899806022644,
-0.03281122073531151,
-0.029482584446668625,
-0.025578338652849197,
-0.027771202847361565,
-0.0332043282687664,
-0.002334121847525239,
0.15789452195167542,
-0.048433441668748856,
0.09890329092741013,
-0.027305716648697853,
-0.03003678470849991,
-0.02338361367583275,
-0.03354298695921898,
0.04188181832432747,
0.030707893893122673,
-0.07813887298107147,
-0.018427139148116112,
-0.062406156212091446,
0.04865537956357002,
-0.038779307156801224,
0.01966872066259384,
-0.033642400056123734,
-0.04119597002863884,
0.01535995863378048,
-0.017551520839333534,
-0.021815286949276924,
-0.12171174585819244,
-0.03871218487620354,
-0.007668000645935535,
0.08280175924301147,
-0.009496110491454601,
0.058683883398771286,
0.04609370231628418,
0.04132888466119766,
-0.0068814377300441265,
0.04125829413533211,
0.09058919548988342,
-0.033060718327760696,
-0.06527819484472275,
0.02494574338197708,
0.011038007214665413,
-0.007617331575602293,
-0.05642923340201378,
0.013823304325342178,
0.04850711673498154,
-0.005535581614822149,
-0.009072976186871529,
-0.003270162036642432,
0.014199151657521725,
0.00752653693780303,
0.03717878833413124,
0.0819617211818695,
0.01610558107495308,
0.10809123516082764,
-0.073216512799263,
-0.023153893649578094,
0.07178676873445511,
0.02023439109325409,
0.02968396618962288,
-0.00974794290959835,
-0.09023907035589218,
-0.002634035889059305,
0.02904638648033142,
0.008414074778556824,
0.05489407852292061,
-0.07210967689752579,
0.0018325222190469503,
-0.02372514083981514,
0.030606769025325775,
-0.00876969750970602,
-0.01544921100139618,
0.008455098606646061,
0.013121974654495716,
-0.05856843292713165,
0.017291445285081863,
0.0016268871258944273,
0.03820958361029625,
0.009016549214720726,
-0.28426629304885864,
0.02597973309457302,
0.007308004889637232,
-0.0040888250805437565,
-0.01789008267223835,
0.05897963047027588,
0.0220783744007349,
0.06623915582895279,
-0.06672760099172592,
-0.0184544175863266,
-0.03906907141208649,
0.02326161041855812,
0.0696430578827858,
0.004898684564977884,
-0.048520732671022415,
-0.0011036685900762677,
0.02377069555222988,
-0.010971073061227798,
0.06200532987713814,
-0.03537127375602722,
-0.018793124705553055,
-0.025391986593604088,
0.1049889400601387,
-0.01811165176331997,
-0.023827480152249336,
0.07503914833068848,
0.0005102158174850047,
0.030215177685022354,
-0.05277938023209572,
0.03590647131204605,
0.0044927094131708145,
0.01780129037797451,
0.05191021412611008,
0.005683157127350569,
0.012373974546790123,
-0.0007834974094294012,
-0.05679316073656082,
-0.023454494774341583,
-0.018893113359808922,
-0.0063984389416873455,
0.03300521895289421,
-0.06844176352024078,
-0.018767792731523514,
-0.01836244761943817,
0.09569834917783737,
-0.09837360680103302,
-0.027617428451776505,
-0.031190838664770126,
0.007282426115125418,
0.024615539237856865,
0.00482855923473835,
-0.055314335972070694,
0.020814385265111923,
0.06663431972265244,
0.010447593405842781,
-0.014974182471632957,
0.0010166859719902277,
0.04819217696785927,
0.00014021460083313286,
-0.01964426413178444,
-0.00945983361452818,
0.012696592137217522,
-0.07084351032972336,
0.05486331507563591,
0.024918047711253166
] |
PMID:8364 | Plasma insulin concentration during physiological variations in immunoreactive plasma secretin. | The effect of endogenous and exogenous secretin on fasting plasma insulin and glucose concentrations in peripheral venous blood was studied. In 10 non-diabetic subjects intragastric instillation of 300 ml 0.1 mol/l hydrochloric acid increased the plasma secretin concentration significantly. This increment did not influence insulin or glucose concentration. Control experiments with intragastric instillation of 300 ml of isotonic saline did not influence the plasma concentration of secretin, insulin or glucose. In four other non-diabetic persons no significant changes were found in plasma insulin or glucose concentration during an i.v. infusion of pure natural porcine secretin in doses of 0.1, 0.3, 1.0 and 3.0 clinical units/kg/h. The results suggest that secretin is without effect on insulin secretion in the fasting normal subject. | Plasma insulin concentration during physiological variations in immunoreactive plasma secretin. The effect of endogenous and exogenous secretin on fasting plasma insulin and glucose concentrations in peripheral venous blood was studied. In 10 non-diabetic subjects intragastric instillation of 300 ml 0.1 mol/l hydrochloric acid increased the plasma secretin concentration significantly. This increment did not influence insulin or glucose concentration. Control experiments with intragastric instillation of 300 ml of isotonic saline did not influence the plasma concentration of secretin, insulin or glucose. In four other non-diabetic persons no significant changes were found in plasma insulin or glucose concentration during an i.v. infusion of pure natural porcine secretin in doses of 0.1, 0.3, 1.0 and 3.0 clinical units/kg/h. The results suggest that secretin is without effect on insulin secretion in the fasting normal subject. | [
0.006362745072692633,
0.03472713753581047,
0.02868337742984295,
0.010868929326534271,
-0.012433953583240509,
-0.023336611688137054,
0.06615103781223297,
0.008865302428603172,
-0.03490280359983444,
-0.03056914173066616,
-0.02879147417843342,
-0.06096203252673149,
0.005731204990297556,
0.047031939029693604,
0.002495503518730402,
-0.016067596152424812,
0.05327070876955986,
-0.03154713660478592,
-0.08459513634443283,
-0.01596730574965477,
-0.0026554286014288664,
-0.03651127591729164,
0.05820535495877266,
-0.0025519037153571844,
-0.0018572694389149547,
-0.0012697301572188735,
-0.01907677948474884,
-0.008120712824165821,
-0.1145096942782402,
-0.18618226051330566,
0.03228076174855232,
0.008142414502799511,
-0.008246878162026405,
-0.011564794927835464,
-0.05753784254193306,
0.015138233080506325,
-0.030463438481092453,
0.01591198891401291,
0.0046377298422157764,
0.0398755706846714,
0.07542625814676285,
-0.002195965498685837,
-0.04107480123639107,
0.03328048437833786,
0.028467142954468727,
-0.004050297196954489,
-0.0006405115127563477,
0.021090248599648476,
0.057703375816345215,
0.053032562136650085,
0.04627789556980133,
0.04744624346494675,
0.034927960485219955,
0.12742330133914948,
0.003994508180767298,
0.012337573803961277,
-0.008170262910425663,
0.026184093207120895,
0.01389953214675188,
0.009018375538289547,
-0.011302298866212368,
0.03925289213657379,
-0.1278437077999115,
0.05073624849319458,
0.07716713100671768,
0.0445246584713459,
-0.08063670247793198,
-0.05252716317772865,
0.06451914459466934,
0.03474977985024452,
-0.06195859983563423,
0.06279726326465607,
-0.0032335210125893354,
0.016634486615657806,
-0.02109142206609249,
-0.006437568925321102,
0.01373662706464529,
0.036851633340120316,
-0.005464487709105015,
0.06402713060379028,
0.013555393554270267,
-0.024415673688054085,
0.039729855954647064,
-0.041920099407434464,
-0.023432321846485138,
-0.01003260351717472,
0.0626823678612709,
-0.03128698840737343,
0.018421920016407967,
-0.04540315270423889,
-0.03951781243085861,
-0.040824923664331436,
-0.07464887946844101,
-0.0103908097371459,
-0.04263458773493767,
0.003431375604122877,
0.02621227689087391,
-0.05847550556063652,
-0.015978358685970306,
0.3638227581977844,
0.024071035906672478,
-0.038111090660095215,
-0.05886142700910568,
-0.010620303452014923,
-0.01336973998695612,
-0.08042089641094208,
-0.005245046690106392,
0.04176904633641243,
-0.00495841633528471,
0.016662243753671646,
0.06507822871208191,
0.038528814911842346,
0.029124295338988304,
-0.013315514661371708,
-0.0112050361931324,
0.06020669639110565,
0.044704318046569824,
-0.025993626564741135,
0.013498395681381226,
0.05926014855504036,
-0.0195166002959013,
0.0124086057767272,
-0.04425961151719093,
-0.027100590988993645,
0.003629772225394845,
0.04809865355491638,
0.008786512538790703,
0.10426362603902817,
0.005012931767851114,
0.0012825375888496637,
0.051532234996557236,
0.014154588803648949,
-0.04561260715126991,
0.007453348487615585,
0.010719617828726768,
-0.02667374536395073,
0.03297905623912811,
0.03323529288172722,
0.06692701578140259,
0.01623792015016079,
-0.03276694193482399,
-0.006524871103465557,
-0.030413426458835602,
-0.0977838784456253,
0.033812928944826126,
0.11451201140880585,
0.06661827117204666,
0.008143585175275803,
0.002165514510124922,
-0.029362281784415245,
0.03276181221008301,
-0.011510859243571758,
-0.03988700360059738,
0.028609244152903557,
-0.003957793582230806,
0.007747000548988581,
-0.02622360736131668,
0.004885322414338589,
-0.008086596615612507,
-0.02789834886789322,
-0.08284948766231537,
-0.03073248639702797,
0.0024096753913909197,
0.03174738213419914,
0.02363637089729309,
0.019985957071185112,
-0.011034871451556683,
-0.023193446919322014,
0.02120177634060383,
0.003757351776584983,
-0.0177793987095356,
-0.012072337791323662,
-0.08211205154657364,
0.003497442463412881,
0.017841847613453865,
-0.02040144056081772,
0.020622840151190758,
-0.00730226980522275,
-0.011052407324314117,
-0.005575015675276518,
-0.034337133169174194,
0.028948236256837845,
0.054521795362234116,
0.052952840924263,
-0.005826879292726517,
-0.009132164530456066,
-0.018054578453302383,
0.06231239438056946,
0.03341654688119888,
0.08960077911615372,
-0.059885378926992416,
0.01314847357571125,
-0.12771284580230713,
-0.006948935799300671,
-0.04437796026468277,
0.018177539110183716,
-0.013012844137847424,
-0.01923731155693531,
-0.02365328185260296,
-0.02295568771660328,
0.05328334867954254,
0.012537815608084202,
0.06346844136714935,
0.07316232472658157,
0.050436608493328094,
0.05113457515835762,
-0.018427947536110878,
-0.07394524663686752,
0.04546327888965607,
0.021839307621121407,
-0.06674668192863464,
0.039698027074337006,
-0.042994532734155655,
0.010087398812174797,
-0.0639149621129036,
0.04106052964925766,
0.018058378249406815,
0.012413399294018745,
0.0659160166978836,
0.009490501135587692,
0.03709152713418007,
-0.07381255179643631,
-0.03465684875845909,
-0.3245018422603607,
-0.03364541754126549,
0.03864729776978493,
-0.03522075340151787,
0.06510602682828903,
-0.03550249710679054,
0.022063177078962326,
0.01983857899904251,
-0.04361247271299362,
-0.0032004828099161386,
0.041935648769140244,
0.05362847447395325,
-0.041649121791124344,
-0.010834778659045696,
0.053070053458213806,
0.030522236600518227,
0.016749095171689987,
-0.07172123342752457,
0.10422322154045105,
-0.016377544030547142,
-0.023535987362265587,
0.0357731394469738,
0.09064224362373352,
-0.005885852500796318,
0.02544131688773632,
-0.03990178182721138,
0.06996413320302963,
-0.08759495615959167,
-0.005415793973952532,
-0.018975812941789627,
-0.043966807425022125,
0.05187919735908508,
-0.010256506502628326,
-0.07359424233436584,
-0.03222677856683731,
-0.07374344766139984,
-0.010523070581257343,
0.054166123270988464,
-0.03699954226613045,
0.04012696444988251,
-0.04970879107713699,
0.028853269293904305,
-0.013250509276986122,
-0.058535732328891754,
-0.050566915422677994,
-0.020862797275185585,
-0.0020789271220564842,
0.05605912208557129,
-0.019052669405937195,
0.018933000043034554,
0.05476406589150429,
-0.028275474905967712,
0.016136234626173973,
0.01709296740591526,
0.037999652326107025,
-0.024019092321395874,
-0.03253358602523804,
-0.04890111833810806,
-0.11309252679347992,
0.06185048446059227,
-0.02304670959711075,
-0.0032090977765619755,
-0.0028232766781002283,
-0.018169967457652092,
-0.027161158621311188,
0.04918608069419861,
0.015011756680905819,
0.030897071585059166,
0.06893768906593323,
0.05941219627857208,
-0.006089041009545326,
0.11659616976976395,
-0.01791221834719181,
0.018649259582161903,
0.07159555703401566,
0.03277898207306862,
-0.0036661687772721052,
-0.07653440535068512,
-0.08452204614877701,
0.025281457230448723,
-0.013430201448500156,
-0.03700323775410652,
0.07346684485673904,
-0.03533357009291649,
0.016860973089933395,
0.04590582847595215,
0.016815360635519028,
0.022962400689721107,
-0.013438737951219082,
0.054673220962285995,
0.00525265047326684,
0.004473918583244085,
-0.05810975283384323,
-0.04976616054773331,
-0.021349167451262474,
0.029707342386245728,
-0.24390248954296112,
0.030689110979437828,
-0.001734091667458415,
-0.02877800166606903,
0.04559210315346718,
0.006061036605387926,
0.019901635125279427,
-0.029971083626151085,
-0.05692162364721298,
0.0027780532836914062,
-0.000921311613637954,
0.00194926792755723,
0.0379229336977005,
0.06058363616466522,
-0.02513761632144451,
-0.026050807908177376,
0.008419845253229141,
-0.04992196336388588,
-0.007452358026057482,
0.013350632041692734,
-0.03953265771269798,
-0.06819520890712738,
0.12455656379461288,
0.01654425822198391,
-0.021053403615951538,
-0.008092525415122509,
0.046367254108190536,
0.040914829820394516,
-0.021419130265712738,
-0.02174297720193863,
0.005209210328757763,
0.014263326302170753,
0.017543962225317955,
0.010065481066703796,
0.013268131762742996,
0.009868824854493141,
0.0014922720147296786,
-0.023753266781568527,
-0.015968507155776024,
0.006082468666136265,
-0.011815286241471767,
-0.03231987729668617,
-0.05059898644685745,
-0.007738819811493158,
0.040803875774145126,
-0.05010411888360977,
0.03413352742791176,
-0.045844659209251404,
0.0316036231815815,
-0.03187558054924011,
-0.03304390236735344,
-0.04227902367711067,
-0.01461610198020935,
0.059116967022418976,
0.03675433620810509,
-0.009092146530747414,
-0.005915910936892033,
-0.06459168344736099,
-0.06562186777591705,
0.012296156026422977,
0.015394754707813263,
-0.02865639701485634,
-0.048482537269592285,
-0.005759410560131073,
0.002956126816570759
] |
PMID:8366 | Stability of the insoluble form of uridine kinase coupled to zn2+ or pb2+ ions. | Partially purified calf brain uridine kinase precipitated by bivalent metal cations has been compared with the soluble enzyme fraction regarding its stability in the presence of inactivating factors. The freeze-dried preparations of uridine kinase precipitaated by Pb2+ or Zn2+ ions, althouth enzymatically highly active, are insoluble in aqueous solutions. The activity of metal-insolubilized enzymes disappears during their preincubation in acidic media or in the presence of silver ions. Also trypsin, chymotrypsin and cathepsin B1 caused decreases in enzyme activity. However, fractions which have been precipitated by metal ions and freeze-dried are stable at high temperatures, whereas the activity of soluble uridine kinase is completely lost. Both unheated metal-ion precipitated uridine kinase preparations and those heated at 100 degrees C are equally sensitive to the feedback inhibition by CTP. | Stability of the insoluble form of uridine kinase coupled to zn2+ or pb2+ ions. Partially purified calf brain uridine kinase precipitated by bivalent metal cations has been compared with the soluble enzyme fraction regarding its stability in the presence of inactivating factors. The freeze-dried preparations of uridine kinase precipitaated by Pb2+ or Zn2+ ions, althouth enzymatically highly active, are insoluble in aqueous solutions. The activity of metal-insolubilized enzymes disappears during their preincubation in acidic media or in the presence of silver ions. Also trypsin, chymotrypsin and cathepsin B1 caused decreases in enzyme activity. However, fractions which have been precipitated by metal ions and freeze-dried are stable at high temperatures, whereas the activity of soluble uridine kinase is completely lost. Both unheated metal-ion precipitated uridine kinase preparations and those heated at 100 degrees C are equally sensitive to the feedback inhibition by CTP. | [
-0.08833431452512741,
-0.026082172989845276,
-0.05557051673531532,
-0.045985158532857895,
-0.022007722407579422,
-0.04508957639336586,
0.03503987193107605,
0.010702659375965595,
-0.03841075673699379,
-0.010377769358456135,
0.007027427665889263,
-0.04845830425620079,
-0.012512896209955215,
0.022100280970335007,
-0.03232872486114502,
-0.013207143172621727,
0.020506329834461212,
-0.009833129122853279,
-0.07969275861978531,
-0.0083180982619524,
-0.060867734253406525,
-0.02810228057205677,
0.04096173122525215,
-0.030377140268683434,
0.0365992970764637,
0.05551213026046753,
0.038663364946842194,
0.04348744451999664,
-0.026694169268012047,
-0.24203942716121674,
0.05903124064207077,
-0.0593910813331604,
0.021419698372483253,
-0.02657250501215458,
0.00849513616412878,
-0.02830967865884304,
0.004526762757450342,
0.007831061258912086,
-0.044335540384054184,
0.0286740493029356,
0.07726389914751053,
0.028335122391581535,
-0.04068642109632492,
0.007089945022016764,
0.015859520062804222,
-0.026096215471625328,
-0.000037153837183723226,
-0.04179473966360092,
0.09306687861680984,
0.005445860326290131,
0.04863975942134857,
0.004338924307376146,
0.02350127138197422,
0.027359291911125183,
0.040108878165483475,
-0.04705487936735153,
0.03800710290670395,
0.033962953835725784,
0.0156104052439332,
0.040852706879377365,
-0.0019565634429454803,
-0.00632198341190815,
-0.10592616349458694,
0.05535796657204628,
0.07345884293317795,
0.003124688984826207,
-0.035526689141988754,
-0.04647912457585335,
0.0524517260491848,
0.04159722849726677,
-0.0345415435731411,
0.07242386043071747,
0.023074332624673843,
0.04924224689602852,
-0.006279375869780779,
-0.05640697851777077,
0.014448059722781181,
-0.0029494487680494785,
0.05217618867754936,
0.04867498204112053,
-0.012687801383435726,
0.003111358266323805,
0.015178651548922062,
-0.024978186935186386,
-0.013745010830461979,
0.005522649735212326,
-0.016100309789180756,
-0.023864449933171272,
-0.03509113937616348,
0.021269984543323517,
-0.004410209134221077,
0.014586734585464,
0.01832122541964054,
-0.01702312007546425,
-0.039986200630664825,
0.09568042308092117,
0.04127569869160652,
0.03908812254667282,
0.01063686516135931,
0.3456130921840668,
-0.015678655356168747,
0.04653174802660942,
-0.0641290545463562,
-0.00499243987724185,
-0.010417739860713482,
-0.03932293877005577,
-0.011248832568526268,
-0.0045914421789348125,
0.008784160949289799,
0.03651644289493561,
0.07083320617675781,
0.03630193695425987,
0.015700682997703552,
-0.04567348584532738,
0.008799013681709766,
0.030004968866705894,
0.00007453956641256809,
0.027770185843110085,
-0.024139421060681343,
-0.003971269819885492,
-0.0598873496055603,
-0.017699100077152252,
0.02179962955415249,
-0.004528375808149576,
0.021680112928152084,
0.017205385491251945,
-0.017067646607756615,
0.07867113500833511,
0.014459687285125256,
0.016973000019788742,
0.06069483608007431,
-0.0011094787623733282,
-0.017907625064253807,
-0.01691303588449955,
-0.010537822730839252,
-0.04991612955927849,
-0.006276357918977737,
-0.08870073407888412,
0.08440151810646057,
-0.021118463948369026,
-0.003940179478377104,
-0.05681530758738518,
-0.06670814752578735,
-0.11407981067895889,
-0.019892215728759766,
0.047378044575452805,
-0.0006781446863897145,
0.03977294638752937,
0.025686070322990417,
-0.02638056129217148,
-0.042512647807598114,
0.06644676625728607,
0.0013023223727941513,
0.01955234445631504,
0.014836376532912254,
0.03763314336538315,
-0.023195765912532806,
0.004617693368345499,
0.05282677337527275,
0.0021587959490716457,
-0.08259733021259308,
-0.03961572051048279,
-0.007298293523490429,
0.08512712270021439,
-0.01651407964527607,
0.04683922976255417,
-0.011510182172060013,
-0.037706319242715836,
0.009598594158887863,
-0.037173300981521606,
-0.04003746062517166,
0.03152287378907204,
-0.04001512750983238,
-0.042087964713573456,
-0.000883092638105154,
-0.008512609638273716,
-0.004561292938888073,
-0.008262176997959614,
0.0101461885496974,
-0.0070762610994279385,
0.027331754565238953,
0.007029365748167038,
-0.011568509042263031,
0.060519326478242874,
0.059754516929388046,
-0.050877463072538376,
-0.04886981472373009,
0.051194824278354645,
0.020063089206814766,
0.06400800496339798,
-0.02984919399023056,
-0.015266829170286655,
-0.07332468777894974,
0.05023111775517464,
-0.0336347334086895,
-0.011155211366713047,
-0.033689312636852264,
0.007788026239722967,
0.028150565922260284,
-0.029683537781238556,
0.10136762261390686,
0.021489260718226433,
-0.023958956822752953,
-0.012453212402760983,
-0.010557543486356735,
0.05252533778548241,
-0.003855691757053137,
0.06014483422040939,
0.0021024008747190237,
-0.023764174431562424,
-0.02841072715818882,
0.001064217765815556,
-0.07088568806648254,
-0.024923719465732574,
-0.03067884035408497,
0.010239618830382824,
-0.002897199708968401,
0.013760428875684738,
0.036307673901319504,
-0.02529480867087841,
0.027957256883382797,
-0.05346529930830002,
-0.05827788636088371,
-0.31853073835372925,
-0.025288641452789307,
-0.01349287573248148,
-0.05682731419801712,
0.09357976913452148,
-0.08031240850687027,
0.013269200921058655,
-0.06893192231655121,
0.030758323147892952,
-0.06820162385702133,
0.04418351873755455,
0.09560099989175797,
0.0061950539238750935,
-0.017039958387613297,
0.006682446226477623,
0.04636250436306,
0.02597193792462349,
-0.054226748645305634,
0.06025487557053566,
0.03392350673675537,
-0.0322188064455986,
-0.05763646587729454,
0.030867107212543488,
0.03374633565545082,
0.06398145854473114,
0.0004095392650924623,
0.07574634253978729,
-0.00458342581987381,
-0.010891711339354515,
-0.016804276034235954,
-0.0063843755051493645,
0.04668815806508064,
-0.03694763779640198,
-0.08467996120452881,
-0.05142708122730255,
0.0010606319410726428,
-0.04214359447360039,
-0.025417592376470566,
-0.025568809360265732,
0.033072128891944885,
-0.019128182902932167,
-0.007623590528964996,
0.0466412678360939,
-0.07629317045211792,
-0.01189827173948288,
0.06213326007127762,
-0.025194667279720306,
0.047337595373392105,
0.014561790972948074,
0.00566459447145462,
0.05899551510810852,
0.006853074766695499,
0.019008956849575043,
0.010742315091192722,
0.014187750406563282,
0.029902223497629166,
-0.04941325634717941,
0.04168996214866638,
-0.021456532180309296,
-0.0022962032817304134,
-0.009110300801694393,
-0.0545634850859642,
-0.029178528115153313,
0.1044100672006607,
0.033851005136966705,
0.03565247729420662,
0.03236115723848343,
-0.014262140728533268,
0.11193424463272095,
0.0027270608115941286,
-0.026524337008595467,
0.10794619470834732,
-0.01778429001569748,
0.03913344815373421,
0.02735581062734127,
0.01899753324687481,
-0.01875120960175991,
-0.01966559700667858,
0.005657817702740431,
0.07211247831583023,
-0.0279191043227911,
0.012478887103497982,
0.08161429315805435,
-0.05733109265565872,
-0.003512385068461299,
0.03641199693083763,
0.013879427686333656,
-0.0412873700261116,
0.0468013770878315,
0.018136583268642426,
0.00778505252674222,
0.010552141815423965,
-0.028457576408982277,
-0.007321022916585207,
0.06677620112895966,
-0.014324119314551353,
-0.2596678137779236,
0.02473687194287777,
-0.02764841914176941,
-0.0348602756857872,
0.0009821292478591204,
0.04206438362598419,
0.043996766209602356,
-0.005255549214780331,
-0.06437485665082932,
0.04837915301322937,
-0.03181137517094612,
0.033808402717113495,
0.04597751796245575,
0.009842362254858017,
-0.03627603128552437,
0.017777729779481888,
0.078447625041008,
-0.06410326808691025,
0.04950375109910965,
-0.04931134730577469,
0.05702205374836922,
-0.059485919773578644,
0.15969306230545044,
0.004233898129314184,
0.016884367913007736,
0.06534097343683243,
0.03143151476979256,
0.004510482307523489,
-0.04812336340546608,
-0.013098690658807755,
-0.00979557354003191,
0.0034318785183131695,
0.03254130110144615,
-0.012108532711863518,
-0.01938321813941002,
0.056996315717697144,
-0.02857423759996891,
0.038199011236429214,
-0.012120756320655346,
-0.014001721516251564,
-0.03821448236703873,
-0.0345296636223793,
-0.0898975133895874,
-0.07141207903623581,
0.011593928560614586,
-0.08539707213640213,
-0.03686920180916786,
-0.04546501487493515,
-0.0007435529842041433,
-0.04736034944653511,
-0.009936974383890629,
-0.030932562425732613,
-0.010269992984831333,
-0.044402316212654114,
-0.024419182911515236,
0.05845720320940018,
0.02470586448907852,
-0.019443247467279434,
-0.00035024352837353945,
0.009703055955469608,
-0.0158702339977026,
0.008913111872971058,
0.03096711076796055,
0.057109370827674866,
0.01454056054353714
] |
PMID:8367 | [Effect of 2-hydroxyestradiol-17beta on NADPH-dependent electron transfer in rat liver microsomes in vitro (author's transl)]. | If rat liver microsomes are incubated with NADPH and 2-hydroxyestradiol-17beta in vitro, the following is observed: 1. Inhibition of lipid peroxidation, 2.inhibition of cytochrome P-450 reduction, and 3.inhibition of cytochrome b5 reduction. Beyond this the catechole inhibits lipid peroxidation of liposomes in vitro. These phenomena can be explained by interaction of different states of oxidation of the estrogen with the NADPH-cytochrome reductase and with 0-2 radicals, which leads to terminal "uncoupling" of microsomal electron transport. | [Effect of 2-hydroxyestradiol-17beta on NADPH-dependent electron transfer in rat liver microsomes in vitro (author's transl)]. If rat liver microsomes are incubated with NADPH and 2-hydroxyestradiol-17beta in vitro, the following is observed: 1. Inhibition of lipid peroxidation, 2.inhibition of cytochrome P-450 reduction, and 3.inhibition of cytochrome b5 reduction. Beyond this the catechole inhibits lipid peroxidation of liposomes in vitro. These phenomena can be explained by interaction of different states of oxidation of the estrogen with the NADPH-cytochrome reductase and with 0-2 radicals, which leads to terminal "uncoupling" of microsomal electron transport. | [
0.0006308458978310227,
0.0018624854274094105,
-0.019107261672616005,
0.04716057330369949,
0.015945829451084137,
-0.05125367268919945,
0.021900519728660583,
0.01609596610069275,
-0.02907942794263363,
-0.04939504340291023,
-0.004539747256785631,
-0.009891032241284847,
-0.008685103617608547,
0.021535590291023254,
-0.0433383509516716,
-0.03100188635289669,
0.008563388139009476,
-0.031058982014656067,
-0.07673893123865128,
0.03599558770656586,
-0.010316278785467148,
0.02946077100932598,
-0.02006983570754528,
0.07052793353796005,
0.004271915648132563,
0.03798951953649521,
-0.0057821753434836864,
-0.011707923375070095,
-0.12306250631809235,
-0.17873409390449524,
0.013031036593019962,
-0.013755487278103828,
-0.048811256885528564,
-0.0020871851593255997,
-0.00688079372048378,
0.01529552973806858,
0.0038978885859251022,
-0.04175364971160889,
-0.02793099917471409,
0.05139758065342903,
0.0176701620221138,
0.003721735905855894,
0.0028058046009391546,
0.02010471373796463,
0.08324932307004929,
-0.019594525918364525,
-0.0005000085802748799,
0.03940204903483391,
0.05693308264017105,
-0.06796101480722427,
-0.020695695653557777,
0.014072850346565247,
-0.01880825310945511,
0.051682859659194946,
0.012130647897720337,
0.015512868762016296,
0.03776515647768974,
0.05762062221765518,
0.0017520010005682707,
0.06569186598062515,
-0.023001333698630333,
0.050269804894924164,
-0.18420755863189697,
0.05752858519554138,
0.0298130065202713,
-0.03172538802027702,
-0.008239010348916054,
0.02708253636956215,
0.039023976773023605,
0.033843427896499634,
-0.0728108286857605,
0.028281277045607567,
-0.008340703323483467,
0.009600280784070492,
0.059712547808885574,
-0.013709750957787037,
0.01986793614923954,
-0.046166032552719116,
0.009238280355930328,
0.01196608878672123,
-0.008187586441636086,
-0.023445913568139076,
-0.028171079233288765,
-0.00398203544318676,
0.002003201050683856,
0.043690938502550125,
-0.035191088914871216,
-0.026553984731435776,
-0.0007434005965478718,
-0.03012053668498993,
-0.0026300123427063227,
-0.040953271090984344,
-0.05729891359806061,
-0.02053641900420189,
-0.08803193271160126,
0.014202304184436798,
0.005078589543700218,
0.02167547307908535,
-0.007489147130399942,
0.3587225675582886,
0.0257024634629488,
0.060697298496961594,
-0.0499844029545784,
-0.02415440045297146,
-0.04440142214298248,
-0.02606027014553547,
0.012017334811389446,
-0.053304824978113174,
0.013038204051554203,
0.06872589886188507,
0.05113068222999573,
-0.0262304600328207,
-0.006822639610618353,
-0.041966378688812256,
-0.02792133390903473,
0.024705704301595688,
0.06802809238433838,
-0.04046884924173355,
-0.04557432979345322,
0.024054856970906258,
-0.012751069851219654,
-0.02248542197048664,
0.08569511771202087,
0.020694591104984283,
0.06144880875945091,
-0.05083587393164635,
0.022686760872602463,
0.07490681856870651,
0.06542694568634033,
0.018384624272584915,
0.060637421905994415,
0.08378688991069794,
-0.03438141196966171,
0.025130972266197205,
-0.03870457783341408,
-0.020102808251976967,
0.022252334281802177,
-0.053059495985507965,
0.014138915576040745,
-0.04110107198357582,
-0.031299177557229996,
-0.058685027062892914,
-0.049009017646312714,
-0.0703859031200409,
-0.020654981955885887,
0.08075007051229477,
0.0889887586236,
0.04019144922494888,
0.048347439616918564,
0.009478849358856678,
0.028566451743245125,
0.04137180745601654,
-0.024739524349570274,
0.05396334454417229,
-0.05034302547574043,
0.0308971144258976,
-0.021370990201830864,
-0.016767332330346107,
-0.017551099881529808,
-0.028894618153572083,
-0.06819583475589752,
-0.009138975292444229,
-0.006290856748819351,
0.07287013530731201,
0.004079131409525871,
-0.01042807474732399,
-0.028877826407551765,
0.00829186849296093,
0.036616064608097076,
0.0054837241768836975,
-0.015665441751480103,
-0.029367024078965187,
0.011879362165927887,
-0.09690304845571518,
-0.051266860216856,
0.03174566105008125,
0.06826584786176682,
0.024761684238910675,
0.03643481433391571,
-0.031609583646059036,
0.010499134659767151,
-0.04868709295988083,
-0.06640978902578354,
0.016625043004751205,
0.035459041595458984,
-0.0015493164537474513,
-0.0301869735121727,
-0.01268350426107645,
0.024981210008263588,
0.0417882464826107,
-0.04235229641199112,
0.04525960981845856,
-0.03638328239321709,
0.011759023182094097,
-0.033059582114219666,
-0.07383791357278824,
-0.06221489608287811,
0.06199583411216736,
-0.0014942773850634694,
0.01219130028039217,
0.133853480219841,
-0.016517264768481255,
0.020650535821914673,
0.04362574964761734,
0.001451882184483111,
0.03251554071903229,
0.04542480781674385,
-0.0033339066430926323,
0.07627154141664505,
-0.010185502469539642,
-0.012333252467215061,
0.00646251393482089,
0.016827072948217392,
0.040301188826560974,
-0.001944874064065516,
0.06930933892726898,
0.026054203510284424,
-0.01757643185555935,
0.07878776639699936,
0.058182477951049805,
0.07831037044525146,
-0.045516688376665115,
-0.046058911830186844,
-0.28987494111061096,
-0.031471241265535355,
0.012800232507288456,
-0.032606493681669235,
0.007215247955173254,
-0.06575465202331543,
0.017571989446878433,
0.016082502901554108,
-0.0026483475230634212,
-0.02956581301987171,
0.036954451352357864,
0.04468385502696037,
-0.07633668184280396,
-0.0654962807893753,
-0.02890831045806408,
0.002804742194712162,
-0.028096957132220268,
-0.0377393439412117,
-0.005374865606427193,
-0.003910932689905167,
0.01792113296687603,
-0.017293576151132584,
-0.009635508060455322,
-0.032946035265922546,
0.11144077777862549,
-0.021877795457839966,
0.09126695245504379,
0.018354086205363274,
-0.05057262256741524,
0.020982548594474792,
0.021893108263611794,
0.04513796791434288,
0.01143792737275362,
-0.10317335277795792,
-0.025979058817029,
-0.06595781445503235,
0.015761490911245346,
-0.02627483941614628,
0.05897778272628784,
0.028000719845294952,
-0.03670496866106987,
0.024641698226332664,
-0.0031689500901848078,
-0.0386253222823143,
-0.03717074170708656,
0.0016228437889367342,
-0.007480117492377758,
-0.001594345667399466,
-0.01428981963545084,
0.019318750128149986,
0.02131347358226776,
0.05894822999835014,
0.007173239719122648,
-0.007710230071097612,
0.1203257367014885,
-0.04323197528719902,
-0.06647256016731262,
0.020326929166913033,
-0.06098713353276253,
-0.013066637329757214,
-0.01953764632344246,
-0.021855367347598076,
-0.006739022210240364,
-0.05804543197154999,
0.01835526153445244,
0.026958169415593147,
-0.032909661531448364,
-0.023757006973028183,
0.037174489349126816,
0.02868017554283142,
0.04219042509794235,
0.11902285367250443,
-0.009095048531889915,
0.028942488133907318,
0.06804810464382172,
0.020138215273618698,
-0.03718438372015953,
-0.03051215223968029,
-0.048583533614873886,
0.0286771971732378,
-0.054154690355062485,
-0.029616715386509895,
0.06394444406032562,
0.01219269447028637,
-0.04553738236427307,
-0.0021961170714348555,
-0.00874994695186615,
-0.06101858243346214,
0.027234692126512527,
0.025422289967536926,
0.00057839450892061,
-0.015578117221593857,
-0.04920288920402527,
-0.057394567877054214,
0.009284071624279022,
0.00018319276568945497,
-0.24902118742465973,
-0.03601865470409393,
0.021009007468819618,
-0.024780938401818275,
-0.050088126212358475,
0.03427188843488693,
0.00311959907412529,
-0.02371744066476822,
-0.030595123767852783,
0.028905455023050308,
-0.00024478844716213644,
0.04825234413146973,
0.1285984069108963,
0.0661582499742508,
0.004174896515905857,
0.0016281531425192952,
0.026892703026533127,
0.03222441300749779,
-0.006059627048671246,
-0.0037448478396981955,
0.0272186491638422,
-0.036415036767721176,
0.09786517173051834,
-0.04391099512577057,
-0.06187769025564194,
-0.014278569258749485,
-0.027669508010149002,
0.025570672005414963,
0.003960089758038521,
0.05445804446935654,
-0.06380996853113174,
0.020296236500144005,
0.07311505079269409,
-0.024735642597079277,
0.0222066231071949,
0.04317975416779518,
-0.03074740432202816,
-0.04070639982819557,
0.0007540005026385188,
0.004232877399772406,
0.006290653254836798,
0.0034560710191726685,
-0.093838632106781,
-0.024922680109739304,
0.07829669862985611,
-0.08400110900402069,
-0.04012542963027954,
0.020308608189225197,
0.02114824391901493,
-0.008722939528524876,
0.012975201942026615,
0.04041143134236336,
0.025021443143486977,
0.07261020690202713,
0.023591138422489166,
0.010518395341932774,
-0.01785884238779545,
-0.004592298995703459,
-0.009282487444579601,
0.01805872656404972,
-0.023003609851002693,
-0.009845132939517498,
-0.06961316615343094,
0.07196220755577087,
0.0694345012307167
] |
PMID:8368 | Purification and characterisation of four polypeptides with neurotoxic activity from Condylactis aurantiaca. | Four toxic polypeptides, Toxins I, II, III and IV were isolated in pure form from the sea anemone Condylactis aurantiaca (Actinaria). Toxin isolation was achieved by alcoholic extraction of the homogenised sea anemones, batchwise adsorption onto cation exchangers, gel filtration on Sephadex G-50 and G-25 and ion-exchange chromatography on SP-Sephadex and QAE-Sephadex. Toxins from Condylactis aurantiaca all contain between 49 and 51 amino acids. Their amino acid compositions were compared to those of the Anemonia sulcata toxins. The toxins were tested on the shore crab Carcinus maenas by intramusclar injection. Crabs react highly sensitively to sea anemone toxins with muscle cramps and paralysis. For Condylactis toxins LD100 ranges from 2 to 6.6 mug/kg Carcinus maenas. | Purification and characterisation of four polypeptides with neurotoxic activity from Condylactis aurantiaca. Four toxic polypeptides, Toxins I, II, III and IV were isolated in pure form from the sea anemone Condylactis aurantiaca (Actinaria). Toxin isolation was achieved by alcoholic extraction of the homogenised sea anemones, batchwise adsorption onto cation exchangers, gel filtration on Sephadex G-50 and G-25 and ion-exchange chromatography on SP-Sephadex and QAE-Sephadex. Toxins from Condylactis aurantiaca all contain between 49 and 51 amino acids. Their amino acid compositions were compared to those of the Anemonia sulcata toxins. The toxins were tested on the shore crab Carcinus maenas by intramusclar injection. Crabs react highly sensitively to sea anemone toxins with muscle cramps and paralysis. For Condylactis toxins LD100 ranges from 2 to 6.6 mug/kg Carcinus maenas. | [
-0.06228033825755119,
-0.06338966637849808,
-0.006571304053068161,
-0.01737184450030327,
0.009894958697259426,
-0.015722990036010742,
0.06982353329658508,
0.03189121186733246,
-0.06251759082078934,
-0.0667695477604866,
0.01918083429336548,
-0.06710367649793625,
-0.028231071308255196,
0.05876459181308746,
0.0005105467280372977,
-0.022951927036046982,
-0.00046451104572042823,
0.0025500506162643433,
0.03003675863146782,
-0.005201359279453754,
0.07326465100049973,
-0.004047148395329714,
0.009053072892129421,
0.0075074899941682816,
-0.005097110755741596,
0.06541720777750015,
0.013510297983884811,
-0.005460818763822317,
-0.04611324891448021,
-0.17120018601417542,
-0.04335009306669235,
0.00036050216294825077,
-0.020927106961607933,
-0.050774600356817245,
-0.017896350473165512,
0.021916156634688377,
0.009343796409666538,
-0.05419442430138588,
0.02272818796336651,
0.06674683094024658,
0.002644873922690749,
0.053385332226753235,
0.02836661972105503,
0.03286806121468544,
0.008531054481863976,
-0.04704675450921059,
-0.05588056892156601,
-0.016820892691612244,
0.12275421619415283,
0.01459499355405569,
0.008172107860445976,
-0.019421346485614777,
0.02274654433131218,
0.05797283723950386,
0.019363394007086754,
-0.04903366044163704,
-0.003671997459605336,
0.02846451662480831,
0.007707881275564432,
0.05235464498400688,
0.03670622780919075,
0.08502362668514252,
-0.08273665606975555,
0.051891494542360306,
0.009546243585646152,
0.04156338796019554,
-0.03285951539874077,
0.062070611864328384,
0.016009457409381866,
0.012439025565981865,
-0.09428822994232178,
0.03332332521677017,
0.04567285254597664,
0.05360952764749527,
0.015406981110572815,
-0.01576761156320572,
-0.03349776938557625,
0.014483784325420856,
0.06433600187301636,
0.000685824838001281,
-0.012277210131287575,
-0.009523115120828152,
0.0005378830246627331,
-0.04213663935661316,
0.029683955013751984,
0.006708996370434761,
0.05728399381041527,
-0.0006683747051283717,
0.02733357436954975,
0.028291955590248108,
0.0041179475374519825,
-0.026261186227202415,
0.005501492880284786,
-0.04046981409192085,
-0.022520996630191803,
0.012536912225186825,
-0.005421322770416737,
-0.026953212916851044,
-0.002431489061564207,
0.33332422375679016,
0.007050108630210161,
-0.020185958594083786,
-0.07373727113008499,
-0.010707763954997063,
0.034129343926906586,
-0.026335928589105606,
0.011480388231575489,
-0.04736156761646271,
0.027629882097244263,
0.05375061556696892,
0.05670962482690811,
0.023873571306467056,
0.0026236753910779953,
-0.007499522063881159,
-0.03311271592974663,
-0.01510052103549242,
0.0123218335211277,
-0.05089076608419418,
-0.00858402345329523,
0.0344095304608345,
-0.029526477679610252,
-0.038566041737794876,
0.06425601989030838,
-0.0003545834042597562,
0.09131652116775513,
0.008783106692135334,
-0.02300415374338627,
0.07856599241495132,
0.08767084032297134,
0.05255557596683502,
0.05058123916387558,
0.02592688426375389,
-0.012928588315844536,
0.023400137200951576,
0.038847412914037704,
0.008755826391279697,
-0.008014966733753681,
-0.007739825639873743,
-0.05184056609869003,
-0.023548202589154243,
-0.09804119169712067,
-0.05972614139318466,
-0.00115787866525352,
-0.06185735762119293,
0.007697449531406164,
0.021159406751394272,
0.039006564766168594,
0.0002868073061108589,
-0.024011725559830666,
0.05436969920992851,
-0.04123791679739952,
0.09622824192047119,
0.010860295966267586,
0.04979369044303894,
0.01490121427923441,
-0.048593100160360336,
-0.056037113070487976,
-0.005242171231657267,
-0.0387219600379467,
-0.021039482206106186,
-0.009688196703791618,
-0.007336223963648081,
0.028221165761351585,
0.11010076105594635,
0.030640121549367905,
-0.06160799041390419,
-0.04774659872055054,
0.004124780185520649,
0.011126167140901089,
-0.011148654855787754,
-0.012018178589642048,
0.04157276451587677,
-0.026922330260276794,
0.03798426315188408,
-0.043804287910461426,
0.009007127955555916,
0.04320451617240906,
-0.015834366902709007,
0.01930045895278454,
0.025978509336709976,
-0.0032515525817871094,
-0.01663259044289589,
-0.042673368006944656,
0.04248660057783127,
0.010763210244476795,
-0.03994804620742798,
-0.022349245846271515,
0.05962739884853363,
0.009657396003603935,
-0.03315414488315582,
-0.04110164940357208,
-0.051471248269081116,
-0.0939878299832344,
0.05450870096683502,
-0.05929063633084297,
-0.036059051752090454,
-0.04382438585162163,
-0.00935315154492855,
0.024925507605075836,
0.01102322619408369,
0.0938873142004013,
0.010453721508383751,
0.0008836729102768004,
0.030932817608118057,
0.012453400529921055,
0.048381201922893524,
-0.025626372545957565,
0.0014598704874515533,
0.0040475367568433285,
-0.004543743561953306,
-0.04392404481768608,
0.009048180654644966,
-0.06303253024816513,
-0.01867719553411007,
-0.005725980270653963,
0.08852884918451309,
0.04524489492177963,
0.02744060382246971,
0.06016107276082039,
0.005538579076528549,
0.01656578667461872,
-0.06295470893383026,
-0.05356336012482643,
-0.30039727687835693,
-0.06628987193107605,
-0.024503294378519058,
-0.05102106183767319,
0.05122126638889313,
-0.07107482105493546,
-0.0025609771255403757,
0.006525462958961725,
0.04362628981471062,
-0.024559209123253822,
0.025832580402493477,
0.03255932405591011,
-0.03696470335125923,
-0.03237352520227432,
0.014946827664971352,
0.10208699852228165,
0.05063382163643837,
0.00850257184356451,
0.028115462511777878,
0.041272807866334915,
-0.022276852279901505,
-0.07065989822149277,
0.019541960209608078,
0.025369498878717422,
0.050836533308029175,
-0.019186388701200485,
0.1333259493112564,
0.09880824387073517,
-0.023091578856110573,
0.003343072719871998,
0.0021039764396846294,
0.05626186728477478,
-0.01970764249563217,
-0.06812185794115067,
-0.019370906054973602,
-0.00790912751108408,
-0.011937074363231659,
-0.026148587465286255,
0.036610860377550125,
-0.061560772359371185,
-0.016600381582975388,
0.03195448964834213,
-0.0070088887587189674,
-0.07977719604969025,
-0.028435703366994858,
-0.020748822018504143,
-0.004197158385068178,
0.0016285391757264733,
0.018872560933232307,
0.05554461106657982,
0.010913969948887825,
0.04938185214996338,
0.013558459468185902,
0.00733518972992897,
-0.020456690341234207,
-0.012406466528773308,
-0.055073145776987076,
-0.0029804660007357597,
-0.07801639288663864,
-0.013868282549083233,
0.043135810643434525,
0.02810763753950596,
-0.0161417368799448,
-0.002088374923914671,
-0.0228458009660244,
0.027021285146474838,
-0.005091756582260132,
-0.03528113663196564,
0.06713856011629105,
-0.003251520451158285,
-0.038625236600637436,
0.11772339046001434,
0.03369069844484329,
-0.07686690986156464,
0.020807361230254173,
0.026266347616910934,
-0.040981996804475784,
-0.1320938616991043,
-0.068470299243927,
0.03714760020375252,
-0.0026187687180936337,
-0.01558307372033596,
0.055991288274526596,
0.02600107155740261,
-0.04397781938314438,
0.0011539750266820192,
-0.0035132088232785463,
-0.039419010281562805,
0.057588931173086166,
-0.030325811356306076,
0.10947833210229874,
-0.00929524376988411,
-0.04728083685040474,
-0.015108807012438774,
-0.002987709827721119,
0.08636630326509476,
-0.26669764518737793,
-0.0031150009017437696,
0.07254349440336227,
0.024487566202878952,
0.006839586887508631,
0.05445136874914169,
0.04627348110079765,
-0.06861850619316101,
0.010161001235246658,
0.033849168568849564,
-0.019879722967743874,
0.03192214295268059,
0.00998775940388441,
-0.0329769067466259,
-0.012547158636152744,
0.03503389284014702,
0.03871551901102066,
-0.013969724997878075,
0.046186987310647964,
-0.002817650558426976,
-0.06174985691905022,
-0.001388966222293675,
0.1474137306213379,
0.04484391584992409,
-0.07057557255029678,
0.021778402850031853,
-0.037362392991781235,
-0.014512268826365471,
-0.0082814060151577,
0.03387851640582085,
-0.027635928243398666,
-0.016779514029622078,
0.02993914857506752,
-0.0798352062702179,
0.053842563182115555,
0.024144571274518967,
-0.0015431515639647841,
-0.013782046735286713,
-0.03587083891034126,
-0.01856756955385208,
-0.047146525233983994,
0.030385775491595268,
-0.0353628471493721,
-0.00788568239659071,
0.060826584696769714,
0.00883653573691845,
-0.07822990417480469,
-0.08884123712778091,
-0.053888630121946335,
0.03327200561761856,
-0.06391170620918274,
-0.0390450581908226,
0.04151841998100281,
-0.009874491952359676,
0.02429034933447838,
-0.012721601873636246,
-0.06130993738770485,
-0.003668280318379402,
0.010791884735226631,
-0.05315457656979561,
0.01164320856332779,
-0.0606955960392952,
-0.03492673859000206,
0.056109797209501266,
0.02917393483221531
] |
PMID:8370 | Hydrolysis-resynthesis equilibrium of the lysine-15--alanine-16 peptide bond in bovine trypsin inhibitor (Kunitz). | Catalytic amounts of bovine beta-trypsin, bovine alpha-chymotrypsin and porcine plasmin establish a true thermodynamic equilibrium between virgin (I) (reactive site Lys15-Ala16 peptide bond intact) and modified (I) (this bond hydrolyzed) bovine trypsin/kallikrein inhibitor (Kunitz). The very slow reaction rates for attaining equilibrium are pH-dependent and differ for different enzymes. Optimal rates are for beta-trypsin at pH 3.75, for alpha-chymotrypsin at pH 5.5, and for plasmin at pH 5.0. Under conditions of optimum pH the equilibrium is reached with the highest rate by plasmin. In 10(-5)M inhibitor solutions the equilibrium concentrations of virgin and modified inhibitor are established by plasmin after almost 300 days starting from either pure virgin or pure modified inhibitor. Thus, the hydrolysis constant KHyd = [I]/[I] is determined to be 0.33 at pH 5.0. In spite of many unsuccessful attempts, this demonstrates that the reactive site peptide bond Lys15-Ala16 in the bovine trypsin inhibitor (Kunitz) can be hydrolyzed by catalytic amounts of endopeptidase. It further confirms that the hydrolyzed Lys15-Ala16 peptide bond in modified inhibitor is subject to thermodynamic control resynthesis. | Hydrolysis-resynthesis equilibrium of the lysine-15--alanine-16 peptide bond in bovine trypsin inhibitor (Kunitz). Catalytic amounts of bovine beta-trypsin, bovine alpha-chymotrypsin and porcine plasmin establish a true thermodynamic equilibrium between virgin (I) (reactive site Lys15-Ala16 peptide bond intact) and modified (I) (this bond hydrolyzed) bovine trypsin/kallikrein inhibitor (Kunitz). The very slow reaction rates for attaining equilibrium are pH-dependent and differ for different enzymes. Optimal rates are for beta-trypsin at pH 3.75, for alpha-chymotrypsin at pH 5.5, and for plasmin at pH 5.0. Under conditions of optimum pH the equilibrium is reached with the highest rate by plasmin. In 10(-5)M inhibitor solutions the equilibrium concentrations of virgin and modified inhibitor are established by plasmin after almost 300 days starting from either pure virgin or pure modified inhibitor. Thus, the hydrolysis constant KHyd = [I]/[I] is determined to be 0.33 at pH 5.0. In spite of many unsuccessful attempts, this demonstrates that the reactive site peptide bond Lys15-Ala16 in the bovine trypsin inhibitor (Kunitz) can be hydrolyzed by catalytic amounts of endopeptidase. It further confirms that the hydrolyzed Lys15-Ala16 peptide bond in modified inhibitor is subject to thermodynamic control resynthesis. | [
-0.10772969573736191,
-0.012993676587939262,
-0.05901698023080826,
0.01672670803964138,
0.011377714574337006,
-0.04595131054520607,
0.028832508251070976,
0.02024932950735092,
0.018846144899725914,
-0.016075436025857925,
0.052802976220846176,
-0.06477882713079453,
0.008805655874311924,
0.04030729830265045,
0.0054044523276388645,
-0.017299536615610123,
-0.0174587182700634,
0.02161489613354206,
-0.07059882581233978,
-0.0029735814314335585,
-0.028936877846717834,
-0.006529578007757664,
0.01915089413523674,
-0.05887864902615547,
0.08267927914857864,
0.058419883251190186,
-0.023307306692004204,
0.007024776190519333,
-0.03417011350393295,
-0.2298498898744583,
-0.0030125342309474945,
-0.0570194274187088,
-0.01629006303846836,
-0.007673662155866623,
-0.022301916033029556,
0.04187009483575821,
-0.049397870898246765,
-0.005401644390076399,
0.015951860696077347,
-0.018377339467406273,
0.06526096910238266,
0.055017706006765366,
0.015734445303678513,
0.011029060930013657,
0.04107966646552086,
0.0027424658183008432,
-0.07991131395101547,
0.06012153625488281,
0.044554371386766434,
0.04801671579480171,
-0.05362817272543907,
-0.032471101731061935,
0.002141339937224984,
0.04880683869123459,
0.0506136454641819,
0.04798997566103935,
0.04113875329494476,
0.04214588552713394,
-0.01629839837551117,
0.040963154286146164,
0.024624407291412354,
0.013207582756876945,
-0.13408972322940826,
0.06009410321712494,
0.06942980736494064,
0.028207967057824135,
0.018701357766985893,
-0.05100702494382858,
0.024685926735401154,
0.054720647633075714,
-0.02307255193591118,
0.04528864100575447,
0.050035614520311356,
-0.011630984023213387,
0.019440896809101105,
-0.008935936726629734,
-0.04593760892748833,
-0.005985545925796032,
0.04468221217393875,
0.03552721068263054,
-0.015599503181874752,
0.0327935628592968,
-0.010782388038933277,
-0.02144889160990715,
0.025430481880903244,
-0.031340342015028,
0.017405251041054726,
-0.011766137555241585,
0.04112632945179939,
0.05889757350087166,
-0.020168183371424675,
-0.041618868708610535,
0.015773948282003403,
-0.034991879016160965,
-0.035103004425764084,
0.06631490588188171,
0.04785937815904617,
-0.03018280118703842,
0.026553694158792496,
0.3189835250377655,
-0.015678156167268753,
0.05618096515536308,
-0.052451301366090775,
-0.05506515875458717,
0.005335504189133644,
-0.025903906673192978,
-0.05866088718175888,
0.022561050951480865,
0.016107816249132156,
0.03646300733089447,
0.008018922060728073,
0.04587259143590927,
0.0487980879843235,
-0.07163966447114944,
0.038822900503873825,
-0.015663929283618927,
0.004871987272053957,
-0.0033486452884972095,
-0.050828512758016586,
0.026705004274845123,
0.0030897101387381554,
0.033591799437999725,
-0.005175941623747349,
0.019610080868005753,
0.03453516215085983,
0.044159021228551865,
-0.01756821945309639,
0.06359871476888657,
0.05822562426328659,
0.03326826170086861,
0.010063757188618183,
0.03591230884194374,
-0.06948396563529968,
-0.020670998841524124,
0.04076036438345909,
-0.019904769957065582,
-0.01009873952716589,
-0.012293463572859764,
0.013544806279242039,
0.027960553765296936,
-0.005027809180319309,
-0.06982051581144333,
-0.04872724786400795,
-0.07932790368795395,
-0.014805947430431843,
0.07027794420719147,
0.0067219785414636135,
0.04565398395061493,
-0.035727519541978836,
-0.00490051694214344,
0.020825963467359543,
0.029749367386102676,
-0.032495319843292236,
0.041441600769758224,
-0.0177760049700737,
0.010070296935737133,
-0.04607964679598808,
0.015305849723517895,
-0.0036079110577702522,
0.017105011269450188,
-0.08646290749311447,
-0.01828485168516636,
-0.0067036948166787624,
0.056201640516519547,
0.018275080248713493,
-0.033783189952373505,
0.02447352558374405,
-0.0056613050401210785,
0.008844724856317043,
-0.02366034686565399,
-0.018923064693808556,
-0.0012771401088684797,
-0.031403545290231705,
-0.02144177444279194,
-0.03511527553200722,
0.008390828967094421,
0.01627170294523239,
-0.0019388907821848989,
0.06171637400984764,
0.06392114609479904,
-0.020268933847546577,
0.04145663604140282,
-0.048698119819164276,
0.007117146626114845,
0.03528706356883049,
-0.03529366850852966,
-0.01936587132513523,
-0.007213559467345476,
-0.013392427936196327,
0.0344025194644928,
-0.026056185364723206,
-0.0017032420728355646,
-0.08017078787088394,
0.047880977392196655,
-0.0678166076540947,
-0.014153302647173405,
-0.07135812938213348,
0.014771557413041592,
0.038823407143354416,
-0.03323878347873688,
0.11424741894006729,
0.008232618682086468,
-0.04848560690879822,
0.04187009856104851,
0.04068176448345184,
0.08644336462020874,
0.006597866769880056,
0.02613011933863163,
-0.010954039171338081,
-0.01426173746585846,
0.007286932319402695,
0.03291037678718567,
-0.09061256051063538,
-0.05376811325550079,
-0.07340648770332336,
0.0380767285823822,
0.022118763998150826,
0.04722664877772331,
0.015641136094927788,
-0.02479170262813568,
0.045265085995197296,
-0.017471279948949814,
-0.03707685321569443,
-0.2949036955833435,
-0.014047728851437569,
-0.013928939588367939,
-0.003344161668792367,
0.09767298400402069,
-0.05680273845791817,
-0.04050305858254433,
-0.03327855467796326,
-0.027763493359088898,
-0.014862488023936749,
0.03319394960999489,
0.06245537847280502,
-0.0294316578656435,
-0.05971122533082962,
0.028709642589092255,
-0.00993247888982296,
0.01729407347738743,
-0.0958496704697609,
0.021802818402647972,
-0.008169284090399742,
-0.05065159499645233,
0.009930250234901905,
0.03058496303856373,
0.005251398775726557,
0.04872873052954674,
0.019531339406967163,
0.11034037172794342,
-0.01751401461660862,
0.03619040548801422,
0.02650953270494938,
-0.019579339772462845,
0.03146962448954582,
-0.021821867674589157,
-0.08039215952157974,
-0.06714893877506256,
-0.01242666132748127,
0.036755677312612534,
-0.05157862603664398,
0.050622712820768356,
-0.06765582412481308,
-0.015532772988080978,
0.06896454840898514,
-0.008211485110223293,
-0.08777542412281036,
-0.04499923065304756,
0.028564617037773132,
0.02067054994404316,
0.01599648781120777,
-0.04301929101347923,
0.02912004478275776,
0.03457384184002876,
0.012460299767553806,
0.04148436710238457,
-0.010369479656219482,
0.06328340619802475,
-0.0026479745283722878,
-0.06269781291484833,
0.0010947388364002109,
-0.03299581632018089,
-0.027637671679258347,
-0.011656408198177814,
-0.014836049638688564,
-0.004037815146148205,
0.08585919439792633,
0.009905548766255379,
-0.016383545473217964,
-0.0017842802917584777,
-0.018555937334895134,
0.03043476678431034,
-0.02187490463256836,
0.02468527853488922,
0.0707746371626854,
-0.020916247740387917,
0.039097461849451065,
0.05214190110564232,
0.03226442262530327,
-0.03264826908707619,
-0.060208309441804886,
-0.0067413365468382835,
0.04937972128391266,
-0.01684536598622799,
-0.07084883004426956,
-0.022083347663283348,
-0.062423378229141235,
0.03214172273874283,
-0.0018185036024078727,
0.03130655735731125,
0.004402460064738989,
-0.036633335053920746,
0.013810570351779461,
-0.010530689731240273,
-0.013085173442959785,
-0.07332579791545868,
0.02694391831755638,
0.03412673994898796,
0.018609706312417984,
-0.3140849173069,
-0.012155411764979362,
0.03599775955080986,
0.03538263961672783,
-0.04108911380171776,
0.07516162842512131,
0.07379419356584549,
-0.07214726507663727,
-0.0784539058804512,
0.02435685507953167,
-0.022658344358205795,
0.041850440204143524,
0.05310285836458206,
0.008191523142158985,
-0.014570663683116436,
-0.027629807591438293,
0.049157481640577316,
0.005501478910446167,
0.010981462895870209,
-0.02806384675204754,
-0.013852876611053944,
-0.05445966497063637,
0.17312180995941162,
0.01986505649983883,
0.011195693165063858,
0.058364320546388626,
0.0006197990151122212,
0.029440855607390404,
-0.02282240055501461,
0.020059911534190178,
0.01297597773373127,
0.03576290234923363,
0.05020569637417793,
-0.04589127376675606,
0.0018499023281037807,
0.04119417816400528,
-0.03319258987903595,
-0.014347264543175697,
-0.016445085406303406,
0.04434936121106148,
-0.0092132817953825,
-0.01895167864859104,
-0.044605765491724014,
-0.001168430782854557,
0.040982600301504135,
-0.11075459420681,
-0.051515623927116394,
-0.013147013261914253,
-0.01874721795320511,
-0.03065720945596695,
-0.028503159061074257,
-0.04683108255267143,
0.0710420310497284,
-0.008064482361078262,
0.025539753958582878,
0.0011911372421309352,
0.02484956942498684,
0.002427157713100314,
-0.07931914180517197,
0.011566066183149815,
0.05470536649227142,
0.023380791768431664,
-0.05098526552319527,
0.06690913438796997,
0.03256058320403099
] |
PMID:8378 | Biological effects of Escherichia coli lipopolysaccharide (LPS) in vivo. I. Selection in the mouse thymus of killer and helper cells. | In the present study we have investigated the biological effects on thymus lymphocytes resulting from Escherichia coli lipopolysaccharide (LPS) treatment in young adult mice. It has been established that LPS induces the following effects: (a) a dose-dependent reduction of thymus weight contemporaneous with a rise in the anti-LPS antibody response; (b) an increase of killer activity of thymus cells; (c) an enhancement of thymocytes helper activity; (d) a reduction of theta-positive cells in the thymus; (e) a cellular depletion in the thymus cortex. These data, indicating that LPS selects in the thymus a population of cells more efficient in expressing both killer and helper functions, are interpreted as caused by an increased rate of cortisol secretion induced by the LPS treatment. | Biological effects of Escherichia coli lipopolysaccharide (LPS) in vivo. I. Selection in the mouse thymus of killer and helper cells. In the present study we have investigated the biological effects on thymus lymphocytes resulting from Escherichia coli lipopolysaccharide (LPS) treatment in young adult mice. It has been established that LPS induces the following effects: (a) a dose-dependent reduction of thymus weight contemporaneous with a rise in the anti-LPS antibody response; (b) an increase of killer activity of thymus cells; (c) an enhancement of thymocytes helper activity; (d) a reduction of theta-positive cells in the thymus; (e) a cellular depletion in the thymus cortex. These data, indicating that LPS selects in the thymus a population of cells more efficient in expressing both killer and helper functions, are interpreted as caused by an increased rate of cortisol secretion induced by the LPS treatment. | [
-0.053216300904750824,
0.018917731940746307,
0.0030308004934340715,
0.04967989772558212,
-0.014003859832882881,
-0.030887942761182785,
0.0769990086555481,
0.05772387981414795,
0.002321608830243349,
-0.040274959057569504,
0.024539943784475327,
-0.0026129530742764473,
0.0016727696638554335,
-0.008421240374445915,
-0.00133914640173316,
-0.04871535301208496,
-0.0017520809778943658,
0.01634160615503788,
-0.12059789150953293,
0.011550377123057842,
-0.06070689857006073,
-0.02881000004708767,
-0.030779141932725906,
-0.024926014244556427,
-0.018605800345540047,
-0.019774366170167923,
-0.00957030151039362,
0.004249646328389645,
-0.11234252154827118,
-0.16780495643615723,
0.014591887593269348,
-0.0746636688709259,
0.01487208716571331,
-0.008555632084608078,
-0.027369536459445953,
0.01982489973306656,
0.0026626726612448692,
-0.03717164695262909,
-0.00444946950301528,
0.04380848631262779,
0.011194299906492233,
0.013275743462145329,
0.01969851925969124,
0.022226782515645027,
0.0016138191567733884,
-0.01032843254506588,
0.0005404383991844952,
0.0016255222726613283,
0.026000043377280235,
0.0038460628129541874,
-0.00431416742503643,
-0.04059894382953644,
0.0390663743019104,
0.12200047820806503,
-0.014573742635548115,
-0.041613247245550156,
-0.015571101568639278,
0.03253854811191559,
0.01919199340045452,
0.08243189007043839,
0.0168171189725399,
-0.023816555738449097,
-0.13521094620227814,
0.08225401490926743,
0.08099230378866196,
-0.0034316908568143845,
-0.025210460647940636,
0.0046118516474962234,
0.051833510398864746,
0.020532209426164627,
-0.08871081471443176,
0.06367667019367218,
0.034211669117212296,
0.042298510670661926,
0.00614973297342658,
0.038704629987478256,
-0.019338494166731834,
-0.05447549745440483,
0.053654979914426804,
0.00713668018579483,
0.022635696455836296,
-0.00204201927408576,
0.03206337243318558,
-0.029406152665615082,
-0.05434776470065117,
-0.005349523387849331,
0.018829362466931343,
-0.09078122675418854,
-0.014713671058416367,
0.03100627288222313,
-0.017229674383997917,
-0.03117378242313862,
-0.010028610937297344,
0.005051689688116312,
-0.044228263199329376,
-0.04214262589812279,
0.015149304643273354,
-0.0896301195025444,
-0.013680381700396538,
0.3813648223876953,
-0.03261161223053932,
-0.022295618429780006,
0.01881512999534607,
-0.011404207907617092,
0.03673684224486351,
0.013203391805291176,
0.003651245264336467,
0.006975274067372084,
-0.03646881505846977,
0.06474774330854416,
0.05493630841374397,
0.010459527373313904,
-0.0024595833383500576,
0.030080940574407578,
-0.03720788285136223,
-0.016276197507977486,
0.08300844579935074,
-0.00967914704233408,
-0.011755090206861496,
0.030633749440312386,
0.02858191728591919,
-0.014347286894917488,
-0.008751713670790195,
-0.06908103078603745,
0.01089826226234436,
0.032235123217105865,
0.04298171401023865,
0.1249866783618927,
0.01938009262084961,
-0.010037706233561039,
0.06508427858352661,
-0.02466365322470665,
-0.030647238716483116,
0.027454949915409088,
0.03827119618654251,
0.029475700110197067,
0.0192771777510643,
0.018222041428089142,
0.029951060190796852,
-0.009390978142619133,
0.023547861725091934,
-0.03842877969145775,
-0.044858913868665695,
-0.1395195871591568,
-0.04432845860719681,
0.12518887221813202,
0.03547493740916252,
0.035575948655605316,
-0.021149028092622757,
0.020553994923830032,
-0.030535023659467697,
0.04857517406344414,
-0.02591363526880741,
0.054061438888311386,
-0.054667528718709946,
0.0476466603577137,
-0.0399022214114666,
-0.0013001126935705543,
0.026941271498799324,
-0.051309891045093536,
-0.09082666039466858,
0.0048204632475972176,
0.017848679795861244,
0.03276491165161133,
0.02522493526339531,
0.02656022273004055,
-0.009787491522729397,
0.015334269031882286,
0.0378454327583313,
0.046508654952049255,
-0.06288142502307892,
0.024128733202815056,
-0.05619959160685539,
-0.02532942220568657,
-0.0904620885848999,
0.026099206879734993,
-0.04840018227696419,
-0.004812507890164852,
0.013766731135547161,
0.0063452995382249355,
-0.00399885093793273,
-0.04085192456841469,
-0.00727968942373991,
0.0003038783324882388,
0.009423558600246906,
-0.017890291288495064,
-0.032084472477436066,
0.0004266276955604553,
0.004594605416059494,
0.005026897415518761,
0.005209826398640871,
-0.041458819061517715,
-0.0516347698867321,
-0.0408199280500412,
-0.05456210672855377,
-0.030321935191750526,
-0.051718343049287796,
0.012747109867632389,
0.0025258390232920647,
-0.03518184274435043,
0.08443619310855865,
0.006069629453122616,
-0.01808304898440838,
0.007108797784894705,
0.012189310044050217,
0.004505760967731476,
0.05498414486646652,
-0.008880850858986378,
0.056259553879499435,
-0.07444296032190323,
-0.04546833410859108,
0.02415255270898342,
0.007403165567666292,
-0.009381202049553394,
-0.04062485322356224,
0.050922513008117676,
0.012950500473380089,
-0.028042636811733246,
0.07886884361505508,
0.004652269184589386,
0.028093814849853516,
0.024645335972309113,
0.020941218361258507,
-0.3048216998577118,
-0.02537279576063156,
-0.004119440447539091,
-0.05704595521092415,
-0.028355708345770836,
-0.04127468541264534,
-0.025127481669187546,
-0.023936321958899498,
0.037080537527799606,
-0.029186565428972244,
0.1148739755153656,
0.058961380273103714,
-0.008170286193490028,
-0.07327505946159363,
0.006072148215025663,
0.05746816471219063,
0.005389249883592129,
-0.04760238900780678,
0.002598903840407729,
0.03565278276801109,
0.03364775702357292,
0.01412829477339983,
0.029808785766363144,
0.021396838128566742,
0.07200706005096436,
-0.01171520259231329,
0.06785332411527634,
0.023353127762675285,
0.03002421371638775,
0.005488020367920399,
0.031513478606939316,
0.04754294082522392,
0.030818868428468704,
-0.07477796822786331,
0.0038984911516308784,
0.019124239683151245,
-0.0319797545671463,
-0.022177690640091896,
0.050235383212566376,
-0.03368271514773369,
-0.0832705944776535,
-0.03996182978153229,
-0.019513994455337524,
-0.07259213924407959,
-0.04101366177201271,
-0.04098896682262421,
0.05720353499054909,
0.08705496042966843,
-0.02387704886496067,
-0.035175710916519165,
-0.02347947098314762,
0.0500992126762867,
0.013688014820218086,
0.03976260498166084,
0.06642021238803864,
0.05525576323270798,
-0.05197839438915253,
-0.015955092385411263,
-0.04285220801830292,
0.002118851291015744,
-0.03238064795732498,
0.01761694625020027,
0.02796279452741146,
0.001409559277817607,
-0.024471193552017212,
0.004256615415215492,
0.012132049538195133,
0.012401028536260128,
-0.010280883871018887,
0.023217501118779182,
-0.02637627348303795,
0.09189583361148834,
0.020240653306245804,
0.03729303181171417,
-0.0008806960540823638,
0.04214465245604515,
-0.05823970586061478,
-0.08211654424667358,
-0.10068944096565247,
0.03976007550954819,
-0.009101838804781437,
-0.02180110104382038,
0.06893548369407654,
-0.07312104105949402,
0.008524761535227299,
0.023477613925933838,
-0.032835349440574646,
0.031076235696673393,
0.020406056195497513,
0.030011296272277832,
-0.05872670188546181,
-0.009613116271793842,
-0.0476159006357193,
-0.010572768747806549,
-0.02490038238465786,
0.026701530441641808,
-0.2443256974220276,
-0.005074688699096441,
0.009401394985616207,
-0.015986930578947067,
-0.03374320641160011,
0.03155030310153961,
-0.009459329769015312,
-0.004210478626191616,
0.0023798232432454824,
0.04138027876615524,
0.027060842141509056,
-0.024179115891456604,
0.025369053706526756,
0.013881441205739975,
-0.027451636269688606,
-0.023370075970888138,
-0.007736080791801214,
-0.002488334896042943,
0.0496242493391037,
0.022366058081388474,
0.023642826825380325,
-0.024554181843996048,
0.1599828600883484,
0.022232023999094963,
0.024744540452957153,
0.0016813335241749883,
0.014008226804435253,
0.009506277740001678,
-0.03272882103919983,
0.038108810782432556,
0.024155601859092712,
-0.010498952120542526,
0.05629832670092583,
0.0036282904911786318,
0.0068181524984538555,
0.03218069300055504,
-0.06719467043876648,
0.02475927211344242,
-0.05758756026625633,
0.08303467929363251,
-0.028250372037291527,
0.04052586853504181,
-0.02018650434911251,
-0.03610200062394142,
0.0782647356390953,
-0.03866344317793846,
0.047525644302368164,
-0.06255967915058136,
0.019730856642127037,
0.030257103964686394,
-0.04354046285152435,
0.03528471291065216,
0.002870833268389106,
-0.0004450388951227069,
0.006877432111650705,
0.0017161293653771281,
0.011357232928276062,
-0.018902160227298737,
0.003890460589900613,
0.010157774202525616,
0.0274429302662611,
0.008408910594880581,
-0.0889912024140358,
0.06456781923770905,
0.09297629445791245
] |
PMID:8381 | Fertility control with sub-dermal silastic capsules containing a new progestin (ST-1435). | The contraceptive action of silastic implants containing a new progestion ST-1435 was investigated in 285 women of reproductive age. A group of 52 women (group A) received three capsules containing each 35 mg of the steroid. A second group of 48 women (group B) received four capsules and a third and larger group of 185 women (group C) received five capsules. No pregnancies occurred during 530 months of use in group A. In 502 months of use recorded for group B, one pregnancy occurred. In group C, 2.142 months were recorded. One pregnancy was reported at the end of 11 months of use and another at the end of 19 months of use. Alterations in the pattern of menstrual bleeding was the most common side effect. | Fertility control with sub-dermal silastic capsules containing a new progestin (ST-1435). The contraceptive action of silastic implants containing a new progestion ST-1435 was investigated in 285 women of reproductive age. A group of 52 women (group A) received three capsules containing each 35 mg of the steroid. A second group of 48 women (group B) received four capsules and a third and larger group of 185 women (group C) received five capsules. No pregnancies occurred during 530 months of use in group A. In 502 months of use recorded for group B, one pregnancy occurred. In group C, 2.142 months were recorded. One pregnancy was reported at the end of 11 months of use and another at the end of 19 months of use. Alterations in the pattern of menstrual bleeding was the most common side effect. | [
0.004953873809427023,
0.019182519987225533,
0.0011809661518782377,
0.01675194688141346,
0.0030775759369134903,
0.031160295009613037,
0.02200191095471382,
0.03411566838622093,
-0.04534667730331421,
0.00615247106179595,
0.010286078788340092,
-0.008657155558466911,
0.0287841334939003,
0.012671668082475662,
-0.017719825729727745,
0.020423930138349533,
-0.007470792159438133,
-0.086106076836586,
-0.04257289320230484,
0.03204872086644173,
0.03938418999314308,
-0.034067343920469284,
-0.06098506972193718,
-0.02647344209253788,
-0.0047685434110462666,
0.022944962605834007,
-0.0182879026979208,
-0.061132997274398804,
-0.04793403297662735,
-0.19208410382270813,
0.019228385761380196,
-0.024243155494332314,
0.030890433117747307,
-0.020286431536078453,
-0.037489958107471466,
-0.012799059972167015,
-0.024939903989434242,
0.012948637828230858,
-0.016425596550107002,
0.062201134860515594,
0.0372372604906559,
0.01903095468878746,
-0.02723655290901661,
0.037119992077350616,
-0.03650897741317749,
0.06955514848232269,
-0.0077162547968328,
-0.025301584973931313,
0.10237676650285721,
0.04108355939388275,
0.054461799561977386,
-0.00015530167729593813,
-0.0019618067890405655,
0.05414745956659317,
-0.04430648684501648,
-0.0301614161580801,
-0.01321195438504219,
0.020004725083708763,
0.011992450803518295,
0.05827132612466812,
-0.02487446740269661,
0.007168416399508715,
-0.14217205345630646,
0.08352687209844589,
-0.00273869838565588,
-0.0058878627605736256,
0.008822127245366573,
-0.06424912065267563,
0.052332811057567596,
0.01627776212990284,
0.026857342571020126,
0.07240951806306839,
0.009216212667524815,
0.011060113087296486,
0.05310744047164917,
0.025605333968997,
0.014803629368543625,
-0.0210726298391819,
0.00858347862958908,
-0.0023177876137197018,
-0.059438034892082214,
0.0593782402575016,
0.030253034085035324,
-0.05151459202170372,
0.009565324522554874,
-0.08625283092260361,
-0.025676868855953217,
0.020653964951634407,
0.05127987638115883,
-0.01554369367659092,
-0.016903547570109367,
-0.014095884747803211,
-0.043612170964479446,
-0.008997157216072083,
-0.03186333552002907,
-0.010592309758067131,
-0.016592100262641907,
-0.010809826664626598,
-0.016221337020397186,
0.38622960448265076,
-0.06387100368738174,
0.005633029621094465,
-0.0208730511367321,
0.0020133787766098976,
0.027429519221186638,
-0.0003281418466940522,
-0.033923063427209854,
-0.05794868245720863,
-0.01171199046075344,
0.0425216443836689,
-0.0063528744503855705,
0.03817778080701828,
0.023824531584978104,
-0.08130835741758347,
-0.027963513508439064,
-0.037998780608177185,
0.035844095051288605,
-0.001531690708361566,
-0.003969825804233551,
-0.046263687312603,
-0.04073074460029602,
0.017955152317881584,
0.04423220455646515,
-0.046421200037002563,
0.01071862317621708,
0.0010051310528069735,
0.022281793877482414,
0.07202018797397614,
-0.036072056740522385,
-0.025580257177352905,
0.027422195300459862,
0.06375561654567719,
-0.08614989370107651,
0.01984812133014202,
0.0008353828452527523,
-0.06529327481985092,
-0.03242288902401924,
-0.06284435093402863,
0.012990938499569893,
-0.03539849817752838,
0.013268661685287952,
-0.0645693838596344,
0.04440631344914436,
-0.04061894491314888,
0.017980435863137245,
0.11162091791629791,
0.014578352682292461,
0.03873344510793686,
0.0661500096321106,
0.05400708690285683,
0.017537718638777733,
0.12510061264038086,
-0.03811371698975563,
-0.007923566736280918,
0.019262487068772316,
-0.028734596446156502,
-0.026492079719901085,
-0.012502430938184261,
-0.038351546972990036,
-0.017622431740164757,
0.01563163287937641,
-0.01910996623337269,
-0.03998642414808273,
0.0810573399066925,
0.03392729163169861,
-0.07982809841632843,
0.008691859431564808,
0.04296175763010979,
0.021813569590449333,
-0.054289016872644424,
0.04070938378572464,
0.014237286522984505,
-0.00524488091468811,
0.018094701692461967,
-0.027115095406770706,
0.04859155789017677,
-0.04526757821440697,
-0.03935273736715317,
0.015265397727489471,
0.03579489886760712,
-0.007958173751831055,
0.052446622401475906,
0.016055721789598465,
-0.0003609790583141148,
-0.020310884341597557,
-0.04754387214779854,
-0.014627886936068535,
0.0012114233104512095,
0.04080016538500786,
-0.009493102319538593,
-0.01142691820859909,
0.014916522428393364,
-0.023478806018829346,
0.06322835385799408,
-0.011758418753743172,
-0.038362372666597366,
-0.06232494115829468,
0.005378225818276405,
-0.00040936324512586,
0.006011764518916607,
0.08255326002836227,
-0.0036458848044276237,
0.044338956475257874,
0.06788379698991776,
0.07834389805793762,
0.0442461334168911,
0.042438577860593796,
-0.03669467940926552,
0.07445230334997177,
0.010517907328903675,
0.006364637054502964,
0.06452613323926926,
0.06406726688146591,
-0.013163295574486256,
0.011197691783308983,
0.0652422085404396,
-0.01668262481689453,
-0.00220735021866858,
0.07681620866060257,
0.005961133167147636,
0.033078633248806,
0.027933426201343536,
0.030499141663312912,
-0.3263561427593231,
-0.0010898540494963527,
0.026389215141534805,
-0.0003800596750807017,
0.012073146179318428,
-0.08199268579483032,
-0.00799660012125969,
-0.039400193840265274,
0.0403587780892849,
-0.07651324570178986,
0.047292888164520264,
0.09292314201593399,
-0.06041562929749489,
-0.0003785297158174217,
0.040379080921411514,
0.025821784511208534,
0.023860948160290718,
-0.04500119760632515,
0.012938070110976696,
-0.04224269092082977,
-0.03282681107521057,
-0.017712365835905075,
-0.031209435313940048,
0.021637573838233948,
0.061659883707761765,
-0.011896798387169838,
0.08555500954389572,
-0.004097920376807451,
-0.05224782973527908,
-0.02948026917874813,
0.023842502385377884,
-0.029940593987703323,
0.02646586485207081,
-0.03897763788700104,
-0.007158457767218351,
0.025916308164596558,
0.0017543581780046225,
-0.0524497851729393,
0.024014240130782127,
-0.006815245840698481,
-0.09227824211120605,
0.02524462714791298,
-0.044573646038770676,
-0.0598083958029747,
-0.03199214115738869,
-0.017024846747517586,
-0.051215652376413345,
0.0029868977144360542,
0.019848473370075226,
0.027439070865511894,
0.016483047977089882,
0.02481791190803051,
-0.015239461325109005,
0.03675079718232155,
0.03407084941864014,
-0.03512321040034294,
-0.05619688704609871,
0.0006224032258614898,
-0.017255611717700958,
0.04213360697031021,
-0.011957530863583088,
-0.009412387385964394,
0.019922731444239616,
-0.05001280456781387,
-0.0406629778444767,
-0.009912561625242233,
-0.03543965518474579,
-0.03361083194613457,
-0.006954183802008629,
-0.07739030569791794,
0.04800199344754219,
0.09515155106782913,
-0.0749877393245697,
0.001993649173527956,
0.03297806903719902,
-0.014717518351972103,
-0.007435772102326155,
0.023018624633550644,
-0.07948527485132217,
-0.017244935035705566,
0.007860453799366951,
0.04014931619167328,
-0.013697752729058266,
-0.006204821169376373,
0.004957339260727167,
0.0213801059871912,
-0.0627133920788765,
0.058557942509651184,
0.019483432173728943,
-0.004773170221596956,
0.026849033311009407,
0.007400390692055225,
0.01907115802168846,
0.016940006986260414,
0.012246677652001381,
0.0038165308069437742,
-0.28745925426483154,
-0.0229366235435009,
0.024913690984249115,
0.024366073310375214,
-0.02867971919476986,
0.005054849665611982,
-0.002278390806168318,
-0.01079723984003067,
-0.013838990591466427,
0.03628276288509369,
0.02391965501010418,
0.07316164672374725,
0.07668280601501465,
-0.0072378539480268955,
0.012228830717504025,
0.014149253256618977,
-0.020039845257997513,
0.0103082787245512,
-0.03653546795248985,
-0.030328890308737755,
0.02363072708249092,
-0.022482115775346756,
0.09436531364917755,
0.04364226013422012,
-0.09095364063978195,
-0.01543690450489521,
0.04425986111164093,
0.08939260989427567,
0.008015986531972885,
0.022300800308585167,
-0.05308738723397255,
0.015835557132959366,
0.02022307738661766,
0.053085289895534515,
-0.02189224772155285,
0.04469196870923042,
-0.024208253249526024,
-0.03439207747578621,
0.01836240291595459,
0.04993034526705742,
0.005733869504183531,
-0.05999814346432686,
-0.018354631960392,
0.012719575315713882,
0.1107058897614479,
-0.08467702567577362,
-0.011276964098215103,
-0.048900894820690155,
0.05223024636507034,
0.05232173949480057,
-0.021914329379796982,
-0.034869857132434845,
0.0022519687190651894,
0.004571438301354647,
-0.01468855794519186,
-0.010512066073715687,
0.029919734224677086,
-0.07404540479183197,
-0.034680403769016266,
0.02956652268767357,
-0.013025504536926746,
-0.055747028440237045,
-0.06345002353191376,
0.05202896520495415,
0.02008059248328209
] |
PMID:8382 | Human semen ribonuclease. Location, properties and inhibition by sodium dodecyl sulfate, zinc sulfate and EDTA. | Optimal conditions were established for RNase activity measurement. The enzyme was measured in human seminal plasma as well as in spermatozoa. Results suggest that sperm enzyme may come from seminal plasma contamination and that RNase may be used as a marker enzyme for seminal plasma contamination. Sodium dodecylsulfate, a reagent utilized to produce the solubilization of the spermatozoa, produced a very strong inhibition of the RNase at low concentrations (530 muM). Zinc sulfate and EDTA also produced inhibition of the RNase activity. Such inhibition may be very useful in future studies of RNA metabolism in human spermatozoa. | Human semen ribonuclease. Location, properties and inhibition by sodium dodecyl sulfate, zinc sulfate and EDTA. Optimal conditions were established for RNase activity measurement. The enzyme was measured in human seminal plasma as well as in spermatozoa. Results suggest that sperm enzyme may come from seminal plasma contamination and that RNase may be used as a marker enzyme for seminal plasma contamination. Sodium dodecylsulfate, a reagent utilized to produce the solubilization of the spermatozoa, produced a very strong inhibition of the RNase at low concentrations (530 muM). Zinc sulfate and EDTA also produced inhibition of the RNase activity. Such inhibition may be very useful in future studies of RNA metabolism in human spermatozoa. | [
-0.02740158885717392,
0.06220261752605438,
-0.03127938508987427,
-0.011436930857598782,
-0.0036622551269829273,
0.012496782466769218,
0.049184881150722504,
0.05740147829055786,
-0.017787184566259384,
-0.012837839312851429,
0.02046690322458744,
-0.0711461678147316,
-0.0041968547739088535,
0.013533679768443108,
-0.0015393850626423955,
-0.0003810347698163241,
-0.020172348245978355,
0.023081693798303604,
-0.060983214527368546,
0.04610397294163704,
0.08472492545843124,
0.041432302445173264,
0.02048259787261486,
-0.06539959460496902,
-0.02286508120596409,
-0.02014816179871559,
-0.06304749101400375,
-0.08127781748771667,
-0.055638737976551056,
-0.18422670662403107,
0.04766249284148216,
-0.05636715888977051,
-0.04344036430120468,
0.00603895727545023,
-0.01605036109685898,
0.010262335650622845,
-0.03268957883119583,
-0.012159697711467743,
-0.02775716595351696,
0.03129423037171364,
0.08719874918460846,
0.01563437096774578,
-0.012326562777161598,
0.022555705159902573,
0.005306454841047525,
0.007127394434064627,
-0.035405054688453674,
-0.011269278824329376,
0.056781381368637085,
0.01738077774643898,
-0.006109307520091534,
-0.004881320986896753,
0.024632666260004044,
-0.004906090907752514,
0.010606667958199978,
-0.012721600942313671,
0.04540763422846794,
0.06027653068304062,
-0.07038775831460953,
0.006814832333475351,
-0.018790733069181442,
-0.02976979687809944,
-0.1309148669242859,
0.08136378973722458,
0.09728715568780899,
-0.02145995944738388,
0.0345064215362072,
-0.05292113497853279,
0.07217440009117126,
0.018478592857718468,
-0.018415212631225586,
0.05991550162434578,
-0.03698726370930672,
0.049421198666095734,
0.01007690280675888,
0.014819354750216007,
0.031736571341753006,
-0.011895520612597466,
0.022929485887289047,
0.023206738755106926,
0.007294639945030212,
-0.05358953773975372,
0.06449848413467407,
0.016893548890948296,
-0.00810167659074068,
0.02851402573287487,
0.05615245923399925,
-0.009069440886378288,
0.027565309777855873,
0.03619518503546715,
-0.019533589482307434,
-0.018048716709017754,
-0.021274268627166748,
-0.0008774836314842105,
-0.04620783403515816,
0.07931511104106903,
0.023224566131830215,
0.016606967896223068,
-0.005134432576596737,
0.40186813473701477,
-0.00649900222197175,
-0.039347801357507706,
-0.11996904015541077,
-0.04986081272363663,
-0.04323089122772217,
-0.0008320788620039821,
-0.02193443663418293,
-0.011286632157862186,
-0.002804191317409277,
0.0583345927298069,
0.07956946641206741,
0.04605269059538841,
0.01942022517323494,
-0.06644198298454285,
0.028553783893585205,
0.03623608872294426,
0.03868537023663521,
0.009823593311011791,
-0.08616353571414948,
0.04426963999867439,
-0.035726290196180344,
0.008789208717644215,
0.009186927229166031,
-0.08151935040950775,
0.03313770890235901,
0.009499902836978436,
0.04356073960661888,
0.11992292106151581,
-0.004044197034090757,
0.008707764558494091,
0.0862237960100174,
0.0024584028869867325,
0.0047920080833137035,
0.009114501997828484,
0.020322419703006744,
-0.021226264536380768,
0.04424474760890007,
0.02707071043550968,
0.07670509070158005,
0.006386794149875641,
-0.003347682533785701,
-0.048309870064258575,
-0.0864754468202591,
-0.050059832632541656,
0.01322584692388773,
0.05561119318008423,
-0.028796426951885223,
-0.007580118719488382,
0.02171807736158371,
-0.024429388344287872,
0.05041029304265976,
0.03122226521372795,
-0.04014093801379204,
0.009218323044478893,
0.008888009004294872,
-0.0195957999676466,
-0.0051199463196098804,
-0.062354426831007004,
-0.02218417078256607,
0.005350508727133274,
-0.07934775203466415,
-0.023242603987455368,
-0.014644484035670757,
0.07896465808153152,
0.00767300371080637,
-0.058853328227996826,
-0.05971076712012291,
0.03264306113123894,
0.03575066477060318,
-0.038489822298288345,
0.008422602899372578,
-0.09359514713287354,
-0.043407224118709564,
-0.028506670147180557,
-0.033511023968458176,
0.02777278609573841,
0.0641663447022438,
0.08739592134952545,
0.0009158396278508008,
0.020942915230989456,
-0.014359483495354652,
-0.028897110372781754,
-0.05965016409754753,
0.024493921548128128,
0.030830444768071175,
-0.04592614248394966,
-0.04570472240447998,
0.01821129210293293,
0.10023941844701767,
0.04746929928660393,
-0.0016265232115983963,
-0.022907478734850883,
-0.05665591359138489,
0.030704768374562263,
0.00009308504377258942,
-0.027105003595352173,
-0.029798466712236404,
0.04495501145720482,
-0.01592598296701908,
-0.03496899455785751,
0.08142977952957153,
0.007619164884090424,
0.038780443370342255,
0.06892425566911697,
0.009921660646796227,
0.06846578419208527,
-0.015009401366114616,
0.0018314053304493427,
0.007919355295598507,
0.029091309756040573,
-0.034980591386556625,
0.015195811167359352,
-0.055515263229608536,
-0.011827616021037102,
-0.08664907515048981,
0.020333237946033478,
0.016784407198429108,
0.008664594031870365,
0.05648498237133026,
-0.02143843099474907,
-0.0006271728780120611,
-0.03875656798481941,
-0.016058379784226418,
-0.2899760901927948,
-0.04664573818445206,
0.024825364351272583,
-0.017379367724061012,
0.05641525238752365,
-0.07703633606433868,
0.002288668882101774,
-0.03880659490823746,
0.01855817250907421,
-0.04047961160540581,
0.05870603397488594,
0.03085140883922577,
0.0005610077641904354,
-0.0065383706241846085,
-0.020754996687173843,
0.05779080092906952,
0.01009917538613081,
-0.02930465154349804,
0.002522219903767109,
0.019695492461323738,
0.03890080749988556,
-0.03871491923928261,
-0.005130282137542963,
0.036655593663454056,
0.12999047338962555,
-0.019204266369342804,
0.13129043579101562,
-0.021427081897854805,
0.031132349744439125,
0.0055878860875964165,
0.032742828130722046,
0.07351619750261307,
-0.0006007251213304698,
-0.1241639107465744,
-0.02838996797800064,
0.0025624698027968407,
-0.05947992578148842,
-0.023948872461915016,
-0.01476622000336647,
-0.02564355358481407,
-0.005944740027189255,
0.006902251858264208,
0.02207580953836441,
-0.029457973316311836,
0.006148701999336481,
-0.017608974128961563,
-0.002366935834288597,
-0.03801833093166351,
0.03138967603445053,
0.026394307613372803,
0.04898947477340698,
0.001956059830263257,
0.01908287964761257,
-0.019315658137202263,
-0.06082265079021454,
0.008938832208514214,
-0.0095282644033432,
0.0003792017523664981,
-0.04419040307402611,
-0.06887836009263992,
0.023823535069823265,
-0.004827562253922224,
-0.043113160878419876,
0.0004575206257868558,
-0.03623898699879646,
-0.02447754330933094,
0.008045452646911144,
-0.033483222126960754,
0.059341318905353546,
0.006385022308677435,
0.030827658250927925,
0.1068631112575531,
-0.07435576617717743,
-0.006493258289992809,
-0.01980052888393402,
0.047864437103271484,
-0.014146100729703903,
-0.08430567383766174,
-0.033418018370866776,
-0.02676873654127121,
-0.013076002709567547,
0.016742458567023277,
0.037691205739974976,
-0.046205125749111176,
-0.010501979850232601,
0.008653827011585236,
0.005248554516583681,
-0.012734903022646904,
0.009324733167886734,
-0.010295595042407513,
0.036313798278570175,
-0.0493888221681118,
-0.0066027045249938965,
-0.01054126862436533,
-0.011327751912176609,
-0.028891567140817642,
-0.23296697437763214,
-0.00028936000308021903,
0.017284726724028587,
-0.006283900700509548,
-0.016000978648662567,
0.03098006173968315,
0.03002689965069294,
-0.03069041483104229,
-0.020794246345758438,
0.06488780677318573,
0.06806551665067673,
0.008261065930128098,
0.0012016065884381533,
0.04370247572660446,
0.02126581221818924,
0.042412176728248596,
0.0245975349098444,
-0.008419083431363106,
-0.0026736583095043898,
-0.03396712988615036,
0.010082725435495377,
-0.08265592157840729,
0.16104494035243988,
0.01660466566681862,
0.03355325758457184,
0.053040239959955215,
0.006044902838766575,
-0.014720873907208443,
-0.048073600977659225,
-0.005800030194222927,
-0.039464887231588364,
0.005636401940137148,
0.009294608607888222,
-0.008244026452302933,
0.021587830036878586,
0.06181822717189789,
-0.0037234402261674404,
-0.019985541701316833,
-0.019511070102453232,
-0.04904041066765785,
-0.01100901234894991,
0.028765525668859482,
-0.09234732389450073,
-0.049182020127773285,
0.03546271473169327,
-0.04007907211780548,
0.011719221249222755,
-0.05043119937181473,
0.037092175334692,
-0.015086840838193893,
0.003802018705755472,
-0.032973408699035645,
0.02872399426996708,
0.013308536261320114,
-0.013152934610843658,
-0.0008245119825005531,
-0.037927400320768356,
0.007099609822034836,
-0.01856098510324955,
0.045109961181879044,
0.01981501281261444,
0.0006738773663528264,
0.0025338283739984035,
0.06683959066867828,
-0.004817205015569925
] |
PMID:8383 | Lack of effects of testosterone on norepinephrine turnover and tyrosine hydroxylase activity of the rat vas deferens and epididymis. | To test the effects of testosterone on some aspects of the metabolism of norepinephrine (NE) in short adrenergic neurons of the male genital tract, orchiectomized rats received 500 mug of testosterone propionate twice daily for up to 18 days. Androgen treatment did not affect the levels, uptake or efflux of NE nor modified the activity of tyrosine hydroxylase in the vas deferens and epididymis. Likewise, formaline-induced fluorescence and ultrastructure of nerve endings remained unchanged after testosterone administration. These data suggest that short adrenergic neurons of male genital tract are relatively insensitive to androgens. | Lack of effects of testosterone on norepinephrine turnover and tyrosine hydroxylase activity of the rat vas deferens and epididymis. To test the effects of testosterone on some aspects of the metabolism of norepinephrine (NE) in short adrenergic neurons of the male genital tract, orchiectomized rats received 500 mug of testosterone propionate twice daily for up to 18 days. Androgen treatment did not affect the levels, uptake or efflux of NE nor modified the activity of tyrosine hydroxylase in the vas deferens and epididymis. Likewise, formaline-induced fluorescence and ultrastructure of nerve endings remained unchanged after testosterone administration. These data suggest that short adrenergic neurons of male genital tract are relatively insensitive to androgens. | [
0.01800333335995674,
0.02857472188770771,
0.0010100905783474445,
-0.0005743401707150042,
-0.00399654870852828,
-0.0058886404149234295,
0.05369995906949043,
0.016640372574329376,
-0.028050657361745834,
-0.060121044516563416,
0.011074413545429707,
-0.015938743948936462,
0.03661317005753517,
0.07165137678384781,
-0.04089449718594551,
0.018118778243660927,
0.003998462576419115,
-0.017133675515651703,
-0.0547843836247921,
-0.008044862188398838,
0.01637299731373787,
0.0072463504038751125,
0.017256923019886017,
-0.027194947004318237,
0.04431929066777229,
-0.03052297607064247,
-0.023526376113295555,
-0.015909019857645035,
-0.048019133508205414,
-0.18420056998729706,
0.05203113332390785,
-0.036779310554265976,
-0.0005237135337665677,
-0.0069191232323646545,
0.0038239052519202232,
-0.014147341251373291,
-0.039172906428575516,
-0.012544112280011177,
0.01718893274664879,
0.0362037755548954,
0.03858717530965805,
0.006586762610822916,
-0.001469214679673314,
0.04302867501974106,
0.010499774478375912,
0.055530671030282974,
-0.10060496628284454,
-0.06042823567986488,
0.08082182705402374,
-0.005044388119131327,
0.009603926911950111,
-0.005028465762734413,
0.0609470009803772,
0.05837489292025566,
0.046390045434236526,
0.012440658174455166,
0.035433221608400345,
0.05009882524609566,
-0.07473427057266235,
0.06006144359707832,
-0.05156314745545387,
0.051439158618450165,
-0.16988727450370789,
0.04561961069703102,
0.05867062881588936,
-0.018564634025096893,
-0.019240669906139374,
0.015562091954052448,
0.04875699058175087,
0.010930475778877735,
-0.05675136670470238,
0.05663304030895233,
-0.006086669396609068,
-0.03570326417684555,
0.0023315667640417814,
-0.0006888413918204606,
-0.04110706225037575,
-0.04341850429773331,
0.041939929127693176,
-0.031775880604982376,
0.0231756791472435,
0.027060501277446747,
0.03441590070724487,
-0.03113912232220173,
0.003476267447695136,
-0.022902412340044975,
0.0010518473573029041,
-0.01541159488260746,
-0.004976428579539061,
0.013521754182875156,
-0.019569534808397293,
-0.02955656126141548,
-0.003397108754143119,
-0.006358693819493055,
-0.03847305849194527,
0.04229894280433655,
0.003306151134893298,
-0.012482468970119953,
-0.037759844213724136,
0.3769757151603699,
0.0020041735842823982,
0.036467358469963074,
-0.05293658748269081,
-0.028938671573996544,
-0.01739564910531044,
0.03496507555246353,
-0.07095035165548325,
-0.03798326477408409,
-0.0559665821492672,
0.06052066758275032,
0.0801103264093399,
0.036599356681108475,
0.013034644536674023,
-0.04297250136733055,
-0.06783748418092728,
-0.030176540836691856,
0.06468435376882553,
0.02795739285647869,
0.014233434572815895,
0.01934155263006687,
-0.057053640484809875,
0.02760491892695427,
-0.003022474003955722,
0.009794875048100948,
0.04481063410639763,
-0.011105455458164215,
-0.009934036061167717,
0.09689266979694366,
0.009415456093847752,
0.036545928567647934,
0.02726459689438343,
0.06269349902868271,
-0.026565615087747574,
0.06126543506979942,
0.02216300182044506,
0.009849457070231438,
0.04913560673594475,
-0.0018605748191475868,
-0.029709601774811745,
-0.023583687841892242,
-0.038394853472709656,
-0.05340803414583206,
-0.04003079608082771,
-0.0633491799235344,
-0.019860029220581055,
0.10726094990968704,
0.05910806730389595,
-0.07608912140130997,
-0.017557166516780853,
0.050221528857946396,
-0.010842532850801945,
0.09307008236646652,
-0.030287986621260643,
0.044905588030815125,
-0.02511555515229702,
0.0009215489262714982,
-0.04638779163360596,
-0.013537920080125332,
-0.03615045174956322,
-0.03905881941318512,
-0.04362477734684944,
0.0013483479851856828,
-0.07944458723068237,
0.03618619963526726,
0.05555525794625282,
-0.006600847467780113,
-0.06473515182733536,
0.03797075152397156,
-0.013560029678046703,
-0.028937846422195435,
0.002642302308231592,
0.01849583350121975,
-0.010240654461085796,
-0.045716460794210434,
-0.07364565879106522,
-0.04105105996131897,
0.06520232558250427,
-0.010309863835573196,
0.01916188932955265,
0.02737502194941044,
0.021718621253967285,
-0.09374361485242844,
-0.006459679454565048,
0.0078015197068452835,
-0.0018692708108574152,
0.04236984625458717,
-0.01599709875881672,
0.02371012046933174,
0.021741611883044243,
0.06547628343105316,
0.0021481940057128668,
-0.027001231908798218,
-0.03384194150567055,
-0.04414557293057442,
0.002517915330827236,
-0.034736715257167816,
-0.03878220543265343,
0.047097060829401016,
0.010270723141729832,
0.02526613138616085,
0.09738130867481232,
-0.03267664834856987,
0.05362451821565628,
0.05003635585308075,
-0.0150696886703372,
0.06991802901029587,
-0.023116163909435272,
-0.009260146878659725,
0.07857180386781693,
0.0018919891444966197,
-0.01662362366914749,
0.015875011682510376,
-0.0012233912711963058,
0.029378844425082207,
-0.025318820029497147,
0.04549523815512657,
0.01739838346838951,
0.004627106245607138,
0.09921324253082275,
-0.021723048761487007,
0.06583021581172943,
-0.026287248358130455,
0.018941355869174004,
-0.28415796160697937,
-0.05453728511929512,
0.005285170394927263,
-0.032562337815761566,
0.06169816851615906,
-0.13478702306747437,
0.02063460275530815,
-0.05821308493614197,
0.04807629436254501,
-0.010658817365765572,
0.031182648614048958,
0.06084264814853668,
0.003829675493761897,
-0.025679463520646095,
0.010957973077893257,
0.07838689535856247,
0.07839971780776978,
-0.06008082628250122,
0.012672391720116138,
0.004846202675253153,
0.010577919892966747,
0.0009014869574457407,
0.008002041839063168,
-0.011222738772630692,
0.020730992779135704,
-0.05146265774965286,
0.08608359098434448,
-0.009829016402363777,
-0.0077792005613446236,
-0.020464787259697914,
0.01357579417526722,
0.03676142171025276,
0.0015207631513476372,
-0.13445983827114105,
0.03174866363406181,
-0.03365432471036911,
-0.006313717924058437,
0.006003269460052252,
-0.01576492190361023,
-0.023786764591932297,
-0.09678464382886887,
-0.01431389432400465,
0.012812633067369461,
-0.04121292009949684,
-0.025594651699066162,
-0.026371745392680168,
0.04241569712758064,
-0.003540322184562683,
-0.04610971361398697,
0.007140916306525469,
0.0376332588493824,
-0.032184675335884094,
0.0003764708817470819,
-0.02911304496228695,
-0.01615518145263195,
-0.05279412120580673,
-0.10406777262687683,
0.011449153535068035,
-0.0064110164530575275,
-0.007243208587169647,
0.06122206896543503,
0.008514796383678913,
0.015415982343256474,
-0.05354698747396469,
0.0035916434135288,
0.019181478768587112,
0.004753155168145895,
-0.01443468313664198,
0.037823908030986786,
0.0208321213722229,
0.06462336331605911,
0.11105086654424667,
-0.01073124073445797,
0.02731463871896267,
0.012965278699994087,
-0.02497750148177147,
-0.047639425843954086,
-0.009321455843746662,
-0.060440871864557266,
0.03238960728049278,
-0.03147297352552414,
-0.03302989900112152,
0.07539211213588715,
0.02907727099955082,
-0.03699905797839165,
-0.007863634265959263,
0.010529471561312675,
-0.07037259638309479,
0.05815858393907547,
-0.004355198238044977,
0.029753584414720535,
0.005749155301600695,
-0.009557874873280525,
0.018255097791552544,
0.043554890900850296,
0.02300405129790306,
-0.2754105031490326,
-0.057402219623327255,
0.04657218977808952,
-0.006924238987267017,
-0.03231564164161682,
0.07077321410179138,
-0.026992512866854668,
-0.027040235698223114,
-0.052865467965602875,
0.02665574662387371,
0.021687675267457962,
0.02780952863395214,
0.05594094097614288,
0.03852052614092827,
-0.0563419908285141,
0.049761030822992325,
0.04678966850042343,
-0.0059837945736944675,
0.008060809224843979,
-0.04991663619875908,
0.056778617203235626,
-0.017124181613326073,
0.1071619987487793,
-0.0045398687943816185,
0.003483083099126816,
0.03528524562716484,
-0.017620207741856575,
0.016852084547281265,
0.005720905959606171,
0.014279927127063274,
-0.06378055363893509,
0.03309221565723419,
0.019381072372198105,
-0.03934372216463089,
0.001594703528098762,
0.040036026388406754,
-0.05111702159047127,
-0.014087792485952377,
0.023036690428853035,
0.00319102150388062,
-0.054728519171476364,
0.0161612406373024,
-0.025436589494347572,
-0.0042189653031528,
0.06644345819950104,
-0.07721022516489029,
-0.015412473119795322,
0.014921441674232483,
-0.005696086212992668,
-0.007042038254439831,
0.00979376770555973,
-0.004538997076451778,
0.019915103912353516,
0.09596090018749237,
0.03371189907193184,
0.009285680018365383,
-0.03335442394018173,
-0.03927748277783394,
0.014839165844023228,
0.0061986264772713184,
-0.06954000890254974,
-0.03828806430101395,
-0.06197851896286011,
0.04517621919512749,
0.030550464987754822
] |
PMID:8384 | Fifteen years experience with artificial insemination. | Artificial insemination is today an accepted procedure in circumventing sterility. In recent years this procedure gained much popularity as the number of infants available for adoption steadily decreased due to the liberal abortion laws. A review of 15 years of practiced experience (senior author) is presented. In all described cases (168 women) artificial insemination was performed personally by the author. The high rate of success (80% of the presented cases) is attributed to a very meticulous and highly individualized approach to each case. Review of literature is presented. | Fifteen years experience with artificial insemination. Artificial insemination is today an accepted procedure in circumventing sterility. In recent years this procedure gained much popularity as the number of infants available for adoption steadily decreased due to the liberal abortion laws. A review of 15 years of practiced experience (senior author) is presented. In all described cases (168 women) artificial insemination was performed personally by the author. The high rate of success (80% of the presented cases) is attributed to a very meticulous and highly individualized approach to each case. Review of literature is presented. | [
-0.01590939424932003,
0.008329316973686218,
-0.003930188249796629,
0.004097476601600647,
-0.032808154821395874,
0.026745401322841644,
0.0006135820876806974,
0.012349219992756844,
0.019493820145726204,
0.011774684302508831,
-0.023461967706680298,
-0.060356441885232925,
-0.023837711662054062,
0.01820828951895237,
-0.041255079209804535,
0.021317925304174423,
-0.028685271739959717,
-0.02135096676647663,
0.007288538385182619,
0.10972660034894943,
0.05076880753040314,
0.012432894669473171,
-0.03316446393728256,
-0.029913824051618576,
0.0162656232714653,
0.026678701862692833,
-0.008352639153599739,
-0.009778729639947414,
-0.017461450770497322,
-0.13334447145462036,
-0.005581953097134829,
-0.04164613410830498,
-0.012259354814887047,
0.04276619479060173,
-0.014136054553091526,
0.006804708857089281,
-0.021636534482240677,
0.035713642835617065,
-0.0478801392018795,
0.05221758410334587,
0.013503302820026875,
0.026899943128228188,
-0.026359636336565018,
-0.002672193106263876,
0.006604738999158144,
0.08366906642913818,
-0.02748291566967964,
0.00041251315269619226,
0.0887109786272049,
0.008576886728405952,
0.014866414479911327,
0.06662541627883911,
0.06963713467121124,
0.013213880360126495,
-0.04787047579884529,
-0.046004269272089005,
0.02333080768585205,
0.013289259746670723,
0.02672666497528553,
-0.014644905924797058,
0.02330089546740055,
0.05473089590668678,
-0.09842374920845032,
0.08241605758666992,
0.010679789818823338,
0.032647594809532166,
0.025927718728780746,
-0.07701259106397629,
0.03504856303334236,
-0.031776078045368195,
0.00488493824377656,
0.03290777653455734,
0.003998061176389456,
0.011512688361108303,
0.01139014307409525,
-0.01915917359292507,
0.04337543994188309,
-0.05711252987384796,
-0.02224242314696312,
-0.005267634522169828,
-0.026146121323108673,
-0.04092869535088539,
0.0017444919794797897,
-0.02720494568347931,
-0.0502295084297657,
-0.03327348828315735,
0.00412428705021739,
-0.05299864336848259,
0.04417528584599495,
0.01178046502172947,
-0.030391106382012367,
0.04535255208611488,
-0.0714973509311676,
0.0003722952969837934,
-0.010039552114903927,
0.06757988035678864,
-0.027009107172489166,
-0.0405423603951931,
-0.03876236453652382,
0.37928682565689087,
-0.026774294674396515,
-0.023349814116954803,
-0.04679766669869423,
0.022371796891093254,
-0.004230876918882132,
-0.04028886929154396,
-0.04596322402358055,
-0.0562320277094841,
-0.01197107695043087,
0.011636005714535713,
-0.03882311284542084,
0.020060596987605095,
0.0817365050315857,
-0.013128968887031078,
-0.023695174604654312,
0.01418811921030283,
0.03949160501360893,
0.047936636954545975,
0.014539999887347221,
0.01977422647178173,
0.015910664573311806,
-0.022955521941184998,
-0.016795657575130463,
-0.021681901067495346,
0.007292683701962233,
-0.07485164701938629,
0.05216165632009506,
0.034527022391557693,
-0.04520329460501671,
-0.006297226529568434,
0.07167477160692215,
0.040630996227264404,
-0.043500203639268875,
0.029301326721906662,
0.03391769528388977,
-0.01322915218770504,
-0.01070813275873661,
0.005822430830448866,
0.007146414369344711,
-0.056480761617422104,
0.026894643902778625,
-0.07115843892097473,
-0.0010611517354846,
-0.0858684778213501,
-0.0019075664458796382,
-0.0008241139585152268,
0.017000891268253326,
0.09947033226490021,
-0.018823258578777313,
0.0158227551728487,
0.014077084138989449,
0.03454827144742012,
-0.06361375004053116,
-0.033560216426849365,
0.05656222254037857,
0.011449668556451797,
0.03493177518248558,
0.07806162536144257,
-0.029581021517515182,
0.010192177258431911,
-0.00040465424535796046,
-0.016639525070786476,
-0.04509150609374046,
0.0895622968673706,
0.034581054002046585,
-0.09728517383337021,
-0.02071651816368103,
0.01239648275077343,
0.0265961904078722,
0.0002650543174240738,
0.018082883208990097,
-0.06277454644441605,
-0.04382786527276039,
-0.026949776336550713,
0.009022046811878681,
0.031127268448472023,
-0.002727794460952282,
0.0008005333947949111,
0.0671280026435852,
0.046841397881507874,
0.015428245067596436,
-0.029276270419359207,
0.017937874421477318,
0.04705199599266052,
0.007978943176567554,
-0.051143527030944824,
-0.045432645827531815,
-0.03315382078289986,
0.025518542155623436,
-0.005658415611833334,
-0.017114603891968727,
0.039413321763277054,
-0.03415672853589058,
0.03927524760365486,
0.01957882009446621,
-0.031059833243489265,
-0.029901079833507538,
-0.016706492751836777,
0.0410815104842186,
-0.030821820721030235,
-0.06566363573074341,
-0.038511812686920166,
0.03876132145524025,
0.011794223450124264,
0.06043551117181778,
-0.0058496189303696156,
0.10197462886571884,
-0.09692974388599396,
0.11169598996639252,
0.04380565136671066,
-0.07255539298057556,
0.05663444846868515,
0.001646642223931849,
0.0169883593916893,
0.0011772062862291932,
0.04817802831530571,
0.02206338383257389,
-0.007782619912177324,
0.02624203823506832,
0.008807467296719551,
0.06584767252206802,
-0.004342557862401009,
0.014250502921640873,
-0.33260107040405273,
-0.08042619377374649,
-0.05174224078655243,
-0.03569743409752846,
0.059002481400966644,
-0.12061844021081924,
-0.04519415646791458,
-0.06895167380571365,
-0.036884553730487823,
-0.03048163279891014,
0.06199565902352333,
0.0543072372674942,
-0.007814791053533554,
0.06037260219454765,
0.021566037088632584,
-0.03804229944944382,
0.01653733290731907,
-0.06862747669219971,
0.03648354485630989,
0.052736010402441025,
-0.024493500590324402,
0.03200487419962883,
0.0170390997081995,
0.07067922502756119,
0.031031465157866478,
-0.005722351837903261,
0.10405481606721878,
-0.05352955684065819,
0.012282798066735268,
0.05916764214634895,
-0.0014222257304936647,
-0.036012131720781326,
0.01659516617655754,
-0.0979885458946228,
-0.05786818638443947,
0.009161663241684437,
0.03692900761961937,
-0.06137516722083092,
0.009329641237854958,
0.04593690112233162,
-0.07880820333957672,
-0.006831808481365442,
-0.03537869453430176,
-0.028752539306879044,
-0.022239066660404205,
0.0328071154654026,
-0.0016297356924042106,
-0.002792810322716832,
-0.05949178338050842,
0.08948907256126404,
0.03383076563477516,
0.016579555347561836,
-0.009965615347027779,
0.030050642788410187,
-0.05978803709149361,
-0.048172902315855026,
-0.08399810642004013,
0.015035239979624748,
-0.0169222429394722,
0.06518875062465668,
0.0002548190823290497,
0.04043067619204521,
0.02360144630074501,
-0.037881556898355484,
0.024167759343981743,
-0.044492270797491074,
-0.007679677568376064,
0.019943730905652046,
0.028879787772893906,
-0.037067875266075134,
0.02253243327140808,
0.13423693180084229,
-0.013103920966386795,
-0.06448115408420563,
0.04469549283385277,
0.01092121284455061,
-0.005713767372071743,
-0.0006607798277400434,
-0.030468739569187164,
-0.005041620694100857,
0.044006552547216415,
-0.03515387699007988,
0.0031738360412418842,
-0.0049145095981657505,
0.015655865892767906,
0.0471145361661911,
-0.0892777219414711,
0.0012194560840725899,
0.004489957354962826,
0.01943112164735794,
0.03089129365980625,
-0.028502581641077995,
-0.0685226172208786,
0.027241922914981842,
-0.05118584260344505,
-0.024665910750627518,
-0.2462850958108902,
-0.005392481107264757,
-0.025741806253790855,
0.07652685046195984,
-0.03881813585758209,
-0.0017896489007398486,
-0.034143660217523575,
-0.053693704307079315,
0.029111837968230247,
0.04469381272792816,
-0.00790409091860056,
0.00575299421325326,
0.06141657382249832,
0.03868132457137108,
0.02071867510676384,
-0.05128219351172447,
-0.041237104684114456,
0.02509870007634163,
-0.03369932249188423,
-0.04835215210914612,
0.06195995584130287,
0.01124013215303421,
0.11596041172742844,
-0.0172373466193676,
-0.00045844155829399824,
0.01866612397134304,
0.035908542573451996,
0.018243679776787758,
-0.034490447491407394,
-0.059406254440546036,
0.013549198396503925,
-0.0061308457516133785,
0.013658322393894196,
0.024678409099578857,
0.03589393571019173,
-0.0688897967338562,
-0.03620044142007828,
-0.03888585790991783,
0.03378742188215256,
-0.0006579448818229139,
-0.09284352511167526,
-0.002926941029727459,
0.019409481436014175,
-0.012874355539679527,
0.052273839712142944,
0.02806830033659935,
0.010617028921842575,
-0.03545611351728439,
0.02479695901274681,
0.06358169764280319,
0.020512588322162628,
-0.01907244510948658,
0.037431035190820694,
0.03002065047621727,
0.051771342754364014,
0.017121722921729088,
0.025505203753709793,
-0.020510753616690636,
-0.05571913719177246,
0.0024340213276445866,
0.019958538934588432,
0.0032579514663666487,
-0.004752238746732473,
0.11326757073402405,
-0.011774871498346329
] |
PMID:8385 | Reversible anti-fertility effect by non-occluding oviductal copper coils in the rabbit. | Placement of two 1 cm copper (Cu) coils around the mid-region of each oviduct in a group of five female rabbits with proven fertility, 34 days before mating, virtually prevented fetal implantation in these animals. The oviducts remained patent during a 47 day implantation period and following removal of the Cu coils, does that were remated became pregnant. In two of these does an implantation rate (living fetuses/corpora lutea) of 33% (7/21) was observed and one post-Cu implanted doe had a litter of two. Among four does bearing a single Cu coil on their left oviduct for 28 days prior to mating there was 19% (4/21) implantation of the left side and 56.3% (9/16) on the untreated right side, suggesting a local action by the metal. The results indicate that Cu is an effective anti-fertility agent when implanted around the oviducts and, as a corollary, it is apparent that the metal is not restricted to placement in the uterus in order to prevent pregnancy in rabbits. Non-occluding Cu containing devices may offer a new approach to reversible fertility control in the female that merits further investigation. | Reversible anti-fertility effect by non-occluding oviductal copper coils in the rabbit. Placement of two 1 cm copper (Cu) coils around the mid-region of each oviduct in a group of five female rabbits with proven fertility, 34 days before mating, virtually prevented fetal implantation in these animals. The oviducts remained patent during a 47 day implantation period and following removal of the Cu coils, does that were remated became pregnant. In two of these does an implantation rate (living fetuses/corpora lutea) of 33% (7/21) was observed and one post-Cu implanted doe had a litter of two. Among four does bearing a single Cu coil on their left oviduct for 28 days prior to mating there was 19% (4/21) implantation of the left side and 56.3% (9/16) on the untreated right side, suggesting a local action by the metal. The results indicate that Cu is an effective anti-fertility agent when implanted around the oviducts and, as a corollary, it is apparent that the metal is not restricted to placement in the uterus in order to prevent pregnancy in rabbits. Non-occluding Cu containing devices may offer a new approach to reversible fertility control in the female that merits further investigation. | [
0.0017852609744295478,
0.0074870772659778595,
0.025995202362537384,
0.08908823132514954,
0.019034292548894882,
0.025844555348157883,
0.003690651385113597,
0.042785607278347015,
-0.03096051514148712,
-0.003300184616819024,
-0.047002341598272324,
-0.05479427054524422,
-0.0033373944461345673,
-0.0040151216089725494,
-0.027049612253904343,
-0.0015662758378311992,
-0.06443040817975998,
-0.0136635797098279,
-0.04393156245350838,
0.01587212085723877,
0.029803812503814697,
-0.02383994497358799,
-0.03728010505437851,
-0.027459319680929184,
0.006934169214218855,
0.016301553696393967,
-0.013711814768612385,
-0.002050149952992797,
-0.020391281694173813,
-0.1945418119430542,
-0.033304329961538315,
-0.02403080277144909,
-0.012984082102775574,
0.053247470408678055,
-0.04870057851076126,
0.009692915715277195,
-0.005228908732533455,
0.027620159089565277,
-0.010619296692311764,
0.05092209577560425,
0.06471513956785202,
0.0008830336737446487,
-0.015373613685369492,
0.012814194895327091,
0.021442601457238197,
0.03399676829576492,
0.011137277819216251,
0.0006809773622080684,
0.08237461745738983,
0.014670050702989101,
0.09918678551912308,
0.042469676584005356,
-0.015518357045948505,
0.03877760097384453,
-0.02186684124171734,
-0.007134314626455307,
0.010604636743664742,
0.05570368841290474,
0.02018354833126068,
-0.003597513772547245,
-0.011113636195659637,
0.02530290000140667,
-0.12860281765460968,
0.028499923646450043,
0.03313925489783287,
-0.020334478467702866,
0.03623923286795616,
-0.0970396101474762,
0.10881036520004272,
0.06214269623160362,
0.02418607473373413,
0.016833167523145676,
-0.028770430013537407,
-0.02081206999719143,
0.04991268366575241,
0.008527095429599285,
0.0403161384165287,
-0.02524794638156891,
0.0010306399781256914,
-0.006607700604945421,
-0.06151609867811203,
0.04584241658449173,
-0.0014652935788035393,
-0.04352602735161781,
0.0033806811552494764,
-0.013708507642149925,
-0.003023624885827303,
-0.04027474671602249,
0.0011232979595661163,
-0.014856487512588501,
-0.01438279077410698,
-0.0352342315018177,
-0.03699055686593056,
-0.06891502439975739,
-0.04336187243461609,
0.0014288524398580194,
0.015682676807045937,
-0.03030093014240265,
-0.01110931858420372,
0.40994229912757874,
-0.053804632276296616,
-0.0069659557193517685,
-0.09023536741733551,
-0.0007087370613589883,
0.026652764528989792,
0.013475880026817322,
-0.031709734350442886,
-0.038325484842061996,
0.00017855584155768156,
0.10649164021015167,
0.02600385621190071,
0.03789624944329262,
0.02228379249572754,
-0.043844327330589294,
0.024812376126646996,
0.014700240455567837,
0.03615983948111534,
0.0250664371997118,
-0.005537603050470352,
0.03550912067294121,
-0.062211208045482635,
0.00782341044396162,
0.042336415499448776,
-0.037030261009931564,
0.009195979684591293,
-0.05132393538951874,
0.023692166432738304,
0.0617516003549099,
-0.04481465741991997,
0.0367300771176815,
0.013232668861746788,
0.03580569848418236,
-0.0006208557169884443,
0.004267572425305843,
-0.0203231330960989,
0.0190853513777256,
-0.019595123827457428,
0.0043149408884346485,
0.03214523568749428,
0.0063868495635688305,
0.032641518861055374,
-0.07525723427534103,
0.0072450339794158936,
-0.08377581089735031,
0.04063330218195915,
0.04984555020928383,
0.05537140369415283,
0.03777604177594185,
0.006132013630121946,
-0.006169200874865055,
0.03270942345261574,
0.09189405292272568,
-0.06660585850477219,
0.009849212132394314,
0.00933141354471445,
-0.030579950660467148,
-0.09053345024585724,
0.05534742400050163,
-0.004448427818715572,
0.009221712127327919,
-0.05479817092418671,
-0.0479813776910305,
-0.032946545630693436,
0.062161996960639954,
0.017712421715259552,
-0.10442107170820236,
0.004554822109639645,
0.06456394493579865,
0.00415372708812356,
-0.016371309757232666,
-0.025304656475782394,
-0.0020575884263962507,
-0.0384344607591629,
-0.04702169448137283,
-0.00875567365437746,
-0.015992678701877594,
-0.08200588077306747,
-0.025050653144717216,
0.013406003825366497,
0.05639895424246788,
-0.00039973261300474405,
-0.016690725460648537,
0.014874400570988655,
0.005414527375251055,
-0.022677427157759666,
-0.002525054384022951,
-0.07249757647514343,
-0.00572774326428771,
0.02209727093577385,
-0.021108245477080345,
-0.007645178586244583,
-0.012071272358298302,
-0.09197457879781723,
0.028622915968298912,
-0.001360287074930966,
-0.08136124163866043,
-0.013057141564786434,
-0.010430735535919666,
0.012813866138458252,
-0.0007123544928617775,
0.008494829759001732,
-0.04549150541424751,
0.050732336938381195,
0.07232290506362915,
0.023259121924638748,
0.02737782895565033,
0.07496466487646103,
-0.027939558029174805,
0.04911119490861893,
0.005134463310241699,
-0.012372162193059921,
0.024334467947483063,
0.018748242408037186,
-0.0049153403379023075,
-0.05575329437851906,
0.06850877404212952,
-0.033265575766563416,
-0.026996340602636337,
0.05910918489098549,
0.0009319696691818535,
0.05349669232964516,
-0.040791869163513184,
0.05003611370921135,
-0.34532666206359863,
-0.051445264369249344,
-0.003215633099898696,
0.03750421851873398,
0.06438466161489487,
-0.09569374471902847,
-0.0034799075219780207,
-0.04880740866065025,
0.024165833368897438,
-0.047355759888887405,
0.03205818310379982,
0.09453410655260086,
-0.029082929715514183,
-0.0008828088757582009,
0.015390442684292793,
0.01824662648141384,
0.036061108112335205,
-0.0810949057340622,
-0.002998943207785487,
0.03842973709106445,
-0.01729373261332512,
0.008162321522831917,
0.043987639248371124,
0.025714507326483727,
0.07793828099966049,
0.02581518143415451,
0.12474264949560165,
-0.007529398892074823,
0.01644613780081272,
0.025515170767903328,
0.02483583241701126,
0.0025887154042720795,
-0.001773621654137969,
-0.028954144567251205,
0.016046743839979172,
0.05657367408275604,
0.023305250331759453,
-0.009760583750903606,
0.002933596493676305,
0.023735901340842247,
-0.044204238802194595,
0.02410702034831047,
-0.007831042632460594,
-0.07488396763801575,
-0.02758892998099327,
0.014379763975739479,
-0.030541444197297096,
0.014993862248957157,
0.03404189273715019,
0.040202703326940536,
0.025603873655200005,
0.0006593730649910867,
-0.0026806967798620462,
0.039008691906929016,
0.0012299302034080029,
0.013925108127295971,
-0.0712822899222374,
0.0028090919367969036,
-0.011482705362141132,
0.012574288994073868,
-0.00048188172513619065,
-0.013916226103901863,
0.021570615470409393,
-0.044916607439517975,
-0.03322692587971687,
-0.03857951983809471,
0.0032433040905743837,
-0.003852511290460825,
0.038728516548871994,
-0.004496521782130003,
0.03296509012579918,
0.13963209092617035,
-0.06662353128194809,
0.0028821108862757683,
0.02063056454062462,
0.0047001950442790985,
-0.04682840779423714,
-0.024447785690426826,
-0.04151422902941704,
0.02184361405670643,
0.021977096796035767,
-0.021302463486790657,
-0.00014630361692979932,
-0.026056183502078056,
0.03260202333331108,
-0.020442407578229904,
-0.055234819650650024,
-0.05138137564063072,
0.05182143300771713,
0.010032383725047112,
-0.00036082498263567686,
0.01071513257920742,
-0.05644560977816582,
0.022799955680966377,
0.014015904627740383,
0.004477445501834154,
-0.2864280641078949,
-0.061487022787332535,
0.03508010506629944,
0.025279950350522995,
-0.017501074820756912,
0.0011109990300610662,
0.001779857906512916,
-0.01062092650681734,
-0.034788113087415695,
0.04307067394256592,
-0.038368478417396545,
0.024598272517323494,
0.04974643141031265,
0.06499017030000687,
0.015566964633762836,
0.011721336282789707,
0.05406913161277771,
-0.040901027619838715,
-0.08685729652643204,
-0.0047924271784722805,
0.047047846019268036,
-0.03416943922638893,
0.08726539462804794,
0.03736378997564316,
-0.021896135061979294,
-0.03879382461309433,
-0.008222525008022785,
0.03445851802825928,
-0.009935136884450912,
-0.039943255484104156,
-0.034772325307130814,
-0.0022306321188807487,
0.005160507280379534,
0.0020695424173027277,
0.04538330063223839,
0.03083963692188263,
-0.05872848257422447,
-0.01759679988026619,
0.028726477175951004,
-0.01643993891775608,
-0.022432655096054077,
0.0039002979174256325,
-0.02231864631175995,
-0.030549868941307068,
0.0379614382982254,
-0.04537012055516243,
-0.025732439011335373,
-0.037257805466651917,
0.030720781534910202,
-0.013988680206239223,
-0.032751671969890594,
-0.004539345856755972,
0.024542607367038727,
0.03230900689959526,
-0.009756031446158886,
-0.046856775879859924,
0.010306564159691334,
0.0035442770458757877,
0.03733782097697258,
0.045050088316202164,
0.044764090329408646,
-0.03443194180727005,
-0.013131160289049149,
0.08433537930250168,
0.05449880659580231
] |
PMID:8386 | The effect of exogenous gondadotropins on the pre-implantation period in the rat. | It is well established that the egg implantation in the rat is dependent on a surge of gonadotropin secretion on day 4 of pregnancy. The present experiments were designed in order to demonstrate the possible effect of exogenous administration of gonadotropins during pre-implantation period in pseudopregnant rats, made pregnant after egg transfer. Groups of pseudopregnant rats by sterile mating were injected with different doses of LH and FSH, between 9:00-12:00 A.M. on day 4 of pseudopregnancy; blastocysts were transferred to them in the morning of day 6, instead of day 5. The implantation rate and the number of young born at term were the criteria of the successful extension of the sensitive period of the endometrium by the exogenous gonadotropins. From the results obtained it can be concluded that LH alone acts on the uterine sensitivity by extension of the implantation period. | The effect of exogenous gondadotropins on the pre-implantation period in the rat. It is well established that the egg implantation in the rat is dependent on a surge of gonadotropin secretion on day 4 of pregnancy. The present experiments were designed in order to demonstrate the possible effect of exogenous administration of gonadotropins during pre-implantation period in pseudopregnant rats, made pregnant after egg transfer. Groups of pseudopregnant rats by sterile mating were injected with different doses of LH and FSH, between 9:00-12:00 A.M. on day 4 of pseudopregnancy; blastocysts were transferred to them in the morning of day 6, instead of day 5. The implantation rate and the number of young born at term were the criteria of the successful extension of the sensitive period of the endometrium by the exogenous gonadotropins. From the results obtained it can be concluded that LH alone acts on the uterine sensitivity by extension of the implantation period. | [
0.025466958060860634,
0.0174325592815876,
-0.012368249706923962,
0.004310436546802521,
0.003715396858751774,
-0.027634354308247566,
0.02256537601351738,
-0.01097262091934681,
-0.052347686141729355,
-0.02513367310166359,
-0.0036697806790471077,
-0.002389588626101613,
0.00665700389072299,
0.05291586369276047,
-0.020512830466032028,
-0.008242575451731682,
-0.005382852163165808,
-0.015312070026993752,
-0.08686177432537079,
-0.0026252376846969128,
0.012004861608147621,
-0.025035692378878593,
0.047581881284713745,
0.037426166236400604,
-0.0024282392114400864,
0.05210741609334946,
-0.055353086441755295,
0.025373585522174835,
-0.07547137141227722,
-0.14235235750675201,
-0.010547135956585407,
-0.016706617549061775,
-0.015751488506793976,
0.016217825934290886,
-0.03810575231909752,
0.0029371450655162334,
0.020519403740763664,
-0.017551040276885033,
-0.022226519882678986,
0.06550958007574081,
0.06705163419246674,
0.008997209370136261,
0.006300183478742838,
-0.012597963213920593,
0.032907918095588684,
0.041885506361722946,
-0.019182777032256126,
0.02931230515241623,
0.012515267357230186,
0.009795586578547955,
0.05713087320327759,
0.0022631080355495214,
0.06284980475902557,
0.03813565894961357,
-0.043483342975378036,
0.0170186348259449,
-0.016680030152201653,
0.007930084131658077,
0.020955508574843407,
0.05674680694937706,
0.001055814907886088,
0.04340739920735359,
-0.14468492567539215,
0.06047002226114273,
0.01623448170721531,
-0.001046344405040145,
-0.011644620448350906,
-0.07500969618558884,
0.08624163269996643,
0.06456118822097778,
-0.05544370040297508,
0.05806446075439453,
0.02142854779958725,
-0.04080812633037567,
0.05098003149032593,
-0.011281872168183327,
-0.0030987965874373913,
-0.045466434210538864,
0.006410782225430012,
-0.013038767501711845,
0.041657429188489914,
0.019868455827236176,
0.07720090448856354,
0.011541628278791904,
-0.0475926473736763,
-0.013201436959207058,
-0.024299554526805878,
-0.06518134474754333,
-0.014095582999289036,
0.04614453390240669,
-0.028426513075828552,
-0.03191627189517021,
-0.0013049313565716147,
-0.06286029517650604,
0.018072348088026047,
0.04476733133196831,
0.02212740108370781,
-0.08252274990081787,
0.010698951780796051,
0.34790170192718506,
-0.0705217495560646,
0.0005771205760538578,
-0.07249181717634201,
0.042264409363269806,
0.0026842299848794937,
-0.019907476380467415,
-0.04533235356211662,
0.0015123997582122684,
-0.02674843929708004,
0.057819318026304245,
0.0027845532167702913,
0.001658676192164421,
0.03324119374155998,
-0.04822439327836037,
0.0044942391104996204,
-0.06578672677278519,
0.023699605837464333,
0.021588018164038658,
-0.0023536134976893663,
0.009081068448722363,
0.015248269774019718,
0.007999819703400135,
0.05053039640188217,
-0.056566618382930756,
0.04223821684718132,
-0.025879323482513428,
-0.003716695122420788,
0.09995022416114807,
-0.025368625298142433,
-0.016648251563310623,
0.047674890607595444,
0.056898556649684906,
-0.03412278741598129,
0.028294609859585762,
0.03302842006087303,
0.008070004172623158,
-0.05764007568359375,
-0.07645874470472336,
-0.05720911920070648,
-0.04620647430419922,
0.03739086911082268,
-0.09754674881696701,
-0.018218619748950005,
-0.05703045800328255,
-0.027935631573200226,
0.10087071359157562,
0.027482349425554276,
0.022882740944623947,
0.03965842351317406,
0.013359248638153076,
0.020640388131141663,
0.06783312559127808,
-0.014621760696172714,
0.036139149218797684,
0.01580999605357647,
0.019230086356401443,
-0.06125584617257118,
-0.001883021555840969,
-0.009831573814153671,
-0.02966630645096302,
-0.07333207875490189,
-0.04298171401023865,
-0.02841801382601261,
0.0029188559856265783,
0.060678981244564056,
-0.01987854577600956,
-0.0012373316567391157,
0.03737393021583557,
-0.017123384401202202,
-0.007810208946466446,
-0.05139533057808876,
-0.03238184005022049,
-0.05046229809522629,
-0.04182327911257744,
-0.003251672489568591,
-0.016792289912700653,
0.014096349477767944,
-0.02136991173028946,
0.008761391043663025,
0.05078461393713951,
0.01816818118095398,
-0.056802377104759216,
-0.030305877327919006,
0.019846655428409576,
0.007843718864023685,
-0.04507230967283249,
-0.035844407975673676,
0.04545792192220688,
0.023335685953497887,
0.0077578104101121426,
-0.01938180811703205,
0.01605250872671604,
-0.08268294483423233,
0.01728922687470913,
-0.034423138946294785,
-0.06982117891311646,
-0.011623190715909004,
0.06268054991960526,
0.030034778639674187,
0.008917851373553276,
0.06545174866914749,
-0.006360077299177647,
0.0652543306350708,
0.0583370178937912,
-0.029006818309426308,
0.03246138617396355,
0.04440266266465187,
-0.03360050544142723,
0.07530225068330765,
0.021678339689970016,
-0.03714584931731224,
0.024003243073821068,
0.040090061724185944,
0.03007948398590088,
-0.047743428498506546,
0.08842084556818008,
-0.015746384859085083,
-0.011207884177565575,
0.08028445392847061,
-0.009902252815663815,
0.01767553947865963,
-0.06614640355110168,
0.0569484606385231,
-0.33184003829956055,
-0.04551757499575615,
-0.022465938702225685,
-0.070085808634758,
-0.0013910502893850207,
-0.0684136375784874,
-0.0038473468739539385,
-0.06697487086057663,
0.0070677329786121845,
-0.045829977840185165,
0.05973665416240692,
0.06979885697364807,
-0.02329988218843937,
-0.0006282729445956647,
0.03232363611459732,
0.06487731635570526,
0.0580609031021595,
-0.0253949873149395,
0.050093214958906174,
0.013003514148294926,
-0.056857530027627945,
0.030603919178247452,
-0.020408419892191887,
-0.02118045836687088,
0.0419333279132843,
-0.00890015996992588,
0.07739081233739853,
0.005247476045042276,
-0.045149918645620346,
-0.0398174524307251,
0.014806109480559826,
-0.02488374523818493,
0.003346081357449293,
-0.0823940560221672,
0.0392572246491909,
0.002625210676342249,
0.05321505665779114,
-0.002555116778239608,
0.018234169110655785,
-0.026229077950119972,
-0.08843899518251419,
-0.04978140816092491,
0.0036290292628109455,
-0.06120791658759117,
-0.07814396172761917,
0.019298547878861427,
-0.02404055930674076,
-0.00231642066501081,
0.018475625663995743,
0.04165881872177124,
0.059371884912252426,
0.00039648209349252284,
0.04151061549782753,
-0.018791157752275467,
0.008184665814042091,
-0.03647766634821892,
-0.0727582573890686,
-0.009332925081253052,
-0.03479122743010521,
0.036741577088832855,
0.01912630908191204,
-0.04059223830699921,
-0.022853273898363113,
-0.041942112147808075,
-0.036380935460329056,
-0.02921047993004322,
-0.0318584069609642,
0.04938383400440216,
0.04129404574632645,
0.03742276132106781,
0.016761768609285355,
0.1161196380853653,
-0.04080434888601303,
0.07444333285093307,
0.03709723800420761,
-0.01331130787730217,
-0.04368817061185837,
-0.03780993074178696,
-0.058753639459609985,
0.00443038297817111,
-0.01143359299749136,
-0.039732202887535095,
0.02649076282978058,
0.0016994166653603315,
-0.0021141490433365107,
0.03913910314440727,
-0.04867911338806152,
0.05599739030003548,
0.04313133284449577,
0.06985330581665039,
-0.039116501808166504,
0.0331692174077034,
0.0008685814100317657,
-0.010259751230478287,
0.02024899050593376,
-0.05577291548252106,
-0.317512571811676,
-0.001967324409633875,
0.04562344402074814,
0.020512400195002556,
0.019505420699715614,
0.03492975980043411,
-0.010185858234763145,
-0.04409872740507126,
-0.0365506187081337,
0.04568808525800705,
-0.021137282252311707,
0.010448253713548183,
0.07255939394235611,
0.005552080925554037,
-0.05747588351368904,
0.0013511620927602053,
-0.00483092712238431,
0.045703813433647156,
-0.04115593805909157,
-0.07256900519132614,
0.03614098206162453,
-0.037582237273454666,
0.13999277353286743,
0.03358655422925949,
-0.012263253331184387,
0.00567853357642889,
0.05750279501080513,
0.06320295482873917,
0.014633160084486008,
0.025067608803510666,
0.03630111366510391,
0.048534996807575226,
-0.00008947541937232018,
-0.03500022739171982,
0.024422772228717804,
0.020752351731061935,
-0.00653030164539814,
0.019798655062913895,
0.02143191173672676,
0.03611382842063904,
-0.0032980190590023994,
0.01626419648528099,
-0.018562117591500282,
0.03852023929357529,
0.07298582792282104,
-0.049815524369478226,
-0.012460051104426384,
-0.01602008007466793,
-0.0003364612930454314,
0.06690676510334015,
-0.012897692620754242,
-0.000499470392242074,
0.024752797558903694,
0.010686471126973629,
-0.06590767204761505,
-0.03801918402314186,
0.012728258036077023,
-0.030529532581567764,
0.017784057185053825,
0.01463128998875618,
0.026718830689787865,
0.003896195674315095,
-0.04658886417746544,
0.06743279099464417,
0.049954649060964584
] |
PMID:8387 | Case report: sterility due to sperm autoagglutination. | Autoagglutination in seminal fluid of husbands in three unexplained sterile couples was found in routine semen-analysis. Further exploration showed that sera and seminal fluid of these husbands agglutinate donors' sperm, while their wives' sera did not. Post-coital tests were not significant. After failure of conservative treatment with vitamin C, two couples agreed to AID followed promptly by pregnancies and deliveries. The possible connection between sterility and sperm-auto-agglutination is discussed, stressing the justification for looking at these phenomena more carefully and with more attention. | Case report: sterility due to sperm autoagglutination. Autoagglutination in seminal fluid of husbands in three unexplained sterile couples was found in routine semen-analysis. Further exploration showed that sera and seminal fluid of these husbands agglutinate donors' sperm, while their wives' sera did not. Post-coital tests were not significant. After failure of conservative treatment with vitamin C, two couples agreed to AID followed promptly by pregnancies and deliveries. The possible connection between sterility and sperm-auto-agglutination is discussed, stressing the justification for looking at these phenomena more carefully and with more attention. | [
-0.003554364200681448,
0.07445917278528214,
-0.045251429080963135,
-0.048685312271118164,
0.03211811184883118,
-0.0261551346629858,
0.1255846470594406,
0.04125615581870079,
-0.04645643010735512,
-0.048072051256895065,
-0.003536014584824443,
-0.08578585088253021,
0.021047305315732956,
0.024767514318227768,
0.015010642819106579,
0.0022501754574477673,
-0.05237581953406334,
0.023560361936688423,
-0.05134577304124832,
0.036366306245326996,
0.026530727744102478,
-0.02858034148812294,
-0.02498050406575203,
-0.060086507350206375,
0.01049743965268135,
0.01436211820691824,
-0.015550794079899788,
-0.0176361333578825,
-0.0060297641903162,
-0.16667023301124573,
-0.00969098974019289,
-0.057331930845975876,
-0.060189004987478256,
0.009531529620289803,
0.01818927377462387,
-0.056237488985061646,
-0.023850737139582634,
0.022539781406521797,
-0.022149767726659775,
0.04051477089524269,
0.07098697870969772,
0.02741890959441662,
-0.023430682718753815,
0.03313648700714111,
-0.00015179436013568193,
0.06398364901542664,
0.012558243237435818,
0.047923553735017776,
0.09773765504360199,
0.008957039564847946,
0.02590360678732395,
0.03183244168758392,
0.011808885261416435,
0.07228200137615204,
-0.08917265385389328,
-0.04562576860189438,
-0.01004042848944664,
0.024986444041132927,
0.0010791386011987925,
0.014756579883396626,
-0.01791258156299591,
0.02561098150908947,
-0.09776230901479721,
0.04497683420777321,
0.08733879029750824,
0.025879789143800735,
0.009001385420560837,
-0.031044671311974525,
0.05312848836183548,
0.045114051550626755,
-0.02604896016418934,
0.04777562990784645,
-0.05116434395313263,
0.004858030006289482,
0.004701362922787666,
0.001322516123764217,
0.005235327873378992,
-0.03945847600698471,
-0.025576472282409668,
0.027478594332933426,
-0.009059388190507889,
0.011627724394202232,
0.004309643991291523,
-0.003547526430338621,
-0.03920034319162369,
-0.02833416499197483,
0.0029274909757077694,
-0.07032713294029236,
0.03136047348380089,
0.018501240760087967,
0.001269024913199246,
-0.05317292734980583,
0.010100037790834904,
0.028796356171369553,
-0.08698640018701553,
0.07928857207298279,
-0.021374285221099854,
-0.038210995495319366,
-0.004025368019938469,
0.3810006082057953,
-0.05510810390114784,
-0.031469035893678665,
-0.03430497273802757,
-0.008242614567279816,
0.018741929903626442,
0.004945374559611082,
-0.04910830780863762,
-0.013034675270318985,
0.009537608362734318,
-0.01372985914349556,
0.036590978503227234,
0.02722269855439663,
0.07492347806692123,
-0.06577353924512863,
0.008741357363760471,
0.04262726753950119,
0.05411152169108391,
-0.012272026389837265,
-0.04692232608795166,
0.002316784579306841,
-0.012346637435257435,
0.047412969172000885,
0.0312277153134346,
-0.06330759078264236,
0.03394894301891327,
0.002367598470300436,
0.05052901431918144,
0.082831971347332,
0.028803454712033272,
-0.02178889885544777,
0.03340555354952812,
0.0571768656373024,
-0.00559336319565773,
-0.0005333637818694115,
0.040931347757577896,
0.003459115047007799,
0.021398043259978294,
-0.006422298029065132,
0.0702991709113121,
-0.02181636169552803,
0.019606158137321472,
-0.020835354924201965,
-0.11480891704559326,
-0.08115542680025101,
0.028108669444918633,
0.071063332259655,
-0.031209750100970268,
-0.022612743079662323,
-0.030931249260902405,
-0.0065489173866808414,
0.03403852880001068,
0.06390557438135147,
-0.06537860631942749,
0.027266500517725945,
0.015889281406998634,
0.0430673323571682,
-0.018886804580688477,
0.021662380546331406,
-0.01164498832076788,
0.016223622485995293,
-0.013138915412127972,
0.010182742960751057,
-0.03450073301792145,
0.03761879727244377,
0.06905870139598846,
-0.05603611469268799,
-0.033531639724969864,
-0.022131361067295074,
0.03079817071557045,
-0.037655431777238846,
0.012249838560819626,
-0.031196335330605507,
-0.07147586345672607,
-0.03252475708723068,
-0.03807404264807701,
0.025170784443616867,
-0.008361589163541794,
0.0473913699388504,
-0.012173465453088284,
0.05948780104517937,
-0.00447889044880867,
0.03664183244109154,
0.012866441160440445,
0.03609371557831764,
0.0128589803352952,
-0.032257214188575745,
-0.10111558437347412,
0.03160037100315094,
0.03328223526477814,
0.051318295300006866,
-0.0069979289546608925,
-0.049497343599796295,
-0.03732683137059212,
0.04630983993411064,
0.039816055446863174,
0.03445293754339218,
0.003632213920354843,
-0.06501882523298264,
0.021430552005767822,
-0.03886760398745537,
0.013227100484073162,
-0.019027967005968094,
0.03930063918232918,
0.028521912172436714,
0.028647614642977715,
0.018652386963367462,
0.01539137028157711,
-0.03828901797533035,
0.056306108832359314,
0.055460818111896515,
-0.011644290760159492,
0.02162947505712509,
-0.12985609471797943,
0.06277144700288773,
-0.031955230981111526,
0.06022624671459198,
-0.04431670531630516,
-0.04456096887588501,
0.0031455352436751127,
0.03261250630021095,
0.058810777962207794,
0.024190470576286316,
0.009842677041888237,
-0.2790362238883972,
-0.044510237872600555,
-0.0405116081237793,
-0.049341898411512375,
0.046681422740221024,
-0.06875837594270706,
0.0008431539754383266,
0.008895361796021461,
0.016166120767593384,
-0.05741732195019722,
0.05840054526925087,
0.06678127497434616,
-0.026033496484160423,
0.030085720121860504,
0.010107987560331821,
-0.020068543031811714,
-0.012440846301615238,
-0.021729597821831703,
0.007820622995495796,
0.057515259832143784,
-0.025895776227116585,
-0.02716873772442341,
0.0013864816864952445,
0.06632696092128754,
0.06282737851142883,
-0.03174177557229996,
0.14497849345207214,
-0.005543403793126345,
-0.03576262667775154,
-0.007036183960735798,
-0.0030806874856352806,
0.05412111058831215,
-0.01598173752427101,
-0.04496762901544571,
-0.05146586894989014,
0.01446802169084549,
-0.027341464534401894,
-0.009910589084029198,
-0.04246522858738899,
0.025476323440670967,
-0.06269314885139465,
-0.019033707678318024,
-0.04263557121157646,
0.035979606211185455,
-0.025385625660419464,
0.0322411023080349,
0.02410455048084259,
-0.011487365700304508,
-0.018685661256313324,
0.09065771102905273,
0.030963480472564697,
-0.01744537055492401,
-0.025952734053134918,
0.0005027048173360527,
-0.05945674329996109,
-0.009308191947638988,
-0.06028767675161362,
-0.0366518497467041,
-0.04084429144859314,
-0.007158749271184206,
0.05604243278503418,
0.0327896922826767,
0.012164590880274773,
-0.07009276747703552,
0.003555485513061285,
-0.03315011411905289,
-0.006999637465924025,
-0.009139401838183403,
0.04097827523946762,
0.01547909528017044,
0.04582195356488228,
0.16918672621250153,
-0.08459126949310303,
-0.00037652437458746135,
0.017033632844686508,
0.009014974348247051,
0.026942957192659378,
0.012783223763108253,
-0.07327723503112793,
-0.05587523430585861,
0.0667896419763565,
-0.057849083095788956,
0.025028562173247337,
-0.015212543308734894,
0.03574623540043831,
0.0306484904140234,
-0.10327647626399994,
0.00708063505589962,
-0.03626221418380737,
0.008606852032244205,
-0.011043740436434746,
-0.03239167481660843,
-0.012295306660234928,
-0.024801136925816536,
0.019403256475925446,
0.009617820382118225,
-0.2637835741043091,
-0.011796042323112488,
0.020358821377158165,
0.03110595978796482,
-0.02780243009328842,
-0.009105020202696323,
0.0016531463479623199,
-0.031223168596625328,
-0.06006458401679993,
0.06176593899726868,
0.023721419274806976,
-0.04637386277318001,
0.05840390548110008,
-0.008025328628718853,
0.0068237753584980965,
0.06052226573228836,
0.00008453404734609649,
-0.04667863994836807,
0.005219403654336929,
-0.03650350123643875,
-0.006354305427521467,
-0.026076678186655045,
0.13887637853622437,
0.03094458021223545,
0.019099362194538116,
0.0579141341149807,
0.04116662219166756,
0.048676617443561554,
-0.0019759719725698233,
0.016688508912920952,
-0.00010768783977255225,
0.03520727530121803,
-0.02032698318362236,
-0.0038817781023681164,
-0.007490863557904959,
-0.015441740863025188,
-0.008879547007381916,
-0.051527027040719986,
0.02535724826157093,
-0.008914642967283726,
-0.018735414370894432,
0.012269348837435246,
-0.05941202491521835,
-0.07280557602643967,
0.006811899598687887,
-0.02068376913666725,
0.0026925825513899326,
-0.03914419934153557,
0.07053818553686142,
0.053957708179950714,
0.02204808220267296,
-0.07957467436790466,
0.029021188616752625,
0.025487031787633896,
-0.009408451616764069,
-0.03578273206949234,
0.007818992249667645,
0.00010878310422413051,
0.011072239838540554,
0.041435759514570236,
0.043740276247262955,
0.0003752681950572878,
-0.06975531578063965,
0.05827951803803444,
-0.021596359089016914
] |
PMID:8388 | Distribution and removal of the acrosin of bull spermatozoa. | The effects of Hyamine 2389, Triton X-100, Nadeoxycholate, acetic acid and hypertonic KCl and MgCl2 as well as freezing and thawing and sonication were studied on the solubilization of acrosin from washed bull spermatozoa, from Hyamine-pretreated spermatozoa (devoid of cell and outer acrosome membrane and of acrosomal material) and from isolated acrosomal caps and vesicles. Concurrent ultrastructural changes were observed. Hyamine, Triton, KCl, and acetic acid effectively solubilized acrosin from whole spermatozoa but MgCl2 had a poor effect. The outer acrosome membrane and acrosomal material extracted by Hyamine contained about 35-40% of the total acrosin activity, and three quarters of it was soluble. The rest of acrosin situated in the innter acrosome membrane or equatorial segment was best extracted by hypertonic KCl and MgCl2, but the detergents were ineffective in this case indicating that acrosin is bound differently to the outer and inner parts of acrosome. The opposite effect of MgCl2 on the acrosin activities extracted from these two parts could even be a suggestion of multiple forms of acrosin. The increase of the total acrosin activity during the Hyamine treatment indicates that acrosin is partly in an inactive form. due either to steric hindrance, inhibitor complex of existence of a proacrosin. | Distribution and removal of the acrosin of bull spermatozoa. The effects of Hyamine 2389, Triton X-100, Nadeoxycholate, acetic acid and hypertonic KCl and MgCl2 as well as freezing and thawing and sonication were studied on the solubilization of acrosin from washed bull spermatozoa, from Hyamine-pretreated spermatozoa (devoid of cell and outer acrosome membrane and of acrosomal material) and from isolated acrosomal caps and vesicles. Concurrent ultrastructural changes were observed. Hyamine, Triton, KCl, and acetic acid effectively solubilized acrosin from whole spermatozoa but MgCl2 had a poor effect. The outer acrosome membrane and acrosomal material extracted by Hyamine contained about 35-40% of the total acrosin activity, and three quarters of it was soluble. The rest of acrosin situated in the innter acrosome membrane or equatorial segment was best extracted by hypertonic KCl and MgCl2, but the detergents were ineffective in this case indicating that acrosin is bound differently to the outer and inner parts of acrosome. The opposite effect of MgCl2 on the acrosin activities extracted from these two parts could even be a suggestion of multiple forms of acrosin. The increase of the total acrosin activity during the Hyamine treatment indicates that acrosin is partly in an inactive form. due either to steric hindrance, inhibitor complex of existence of a proacrosin. | [
-0.02162625454366207,
0.028033072128891945,
-0.08073144406080246,
0.011714989319443703,
0.022226981818675995,
0.006528127938508987,
0.005712986923754215,
0.06191223859786987,
-0.03423682972788811,
-0.015075183473527431,
0.009662410244345665,
-0.09065841883420944,
0.09238311648368835,
0.031181981787085533,
-0.02947649545967579,
-0.005351366475224495,
-0.013621946796774864,
0.00009469474025536329,
-0.07459685206413269,
-0.016470571979880333,
0.01180135179311037,
-0.04881901666522026,
-0.002844505477696657,
-0.03163973614573479,
0.05118487775325775,
0.06154482066631317,
-0.03720461577177048,
0.013815555721521378,
-0.03733668476343155,
-0.20349162817001343,
-0.0188315287232399,
0.01763232983648777,
0.01153213158249855,
-0.025232350453734398,
0.030250128358602524,
0.01047151256352663,
-0.02207697555422783,
0.026089724153280258,
-0.031714726239442825,
0.023316996172070503,
0.1512078195810318,
0.060822390019893646,
-0.01135238166898489,
0.00716248108074069,
0.012808838859200478,
0.013362212106585503,
-0.030246257781982422,
-0.03608464077115059,
0.12963978946208954,
0.011772410944104195,
0.038112472742795944,
-0.002916476223617792,
0.027936557307839394,
0.008399385958909988,
0.0015051900409162045,
0.0028968527913093567,
-0.013645965605974197,
0.028815634548664093,
-0.08163569867610931,
0.05697304755449295,
0.03779544681310654,
0.033984676003456116,
-0.11549059301614761,
0.08595027029514313,
0.05967674031853676,
0.008523356169462204,
-0.014157583005726337,
-0.024400915950536728,
0.06429693847894669,
0.04899038374423981,
-0.038655877113342285,
0.026905037462711334,
0.007032182067632675,
-0.05642955005168915,
-0.033802326768636703,
-0.06188623234629631,
0.008555320091545582,
-0.04654807969927788,
0.01475869957357645,
-0.025972064584493637,
-0.028515305370092392,
-0.06004435941576958,
0.018694788217544556,
-0.02428949438035488,
0.006504141725599766,
0.02766588144004345,
-0.036152660846710205,
-0.012117315083742142,
0.04181049019098282,
0.028424745425581932,
0.013989483006298542,
-0.054928284138441086,
0.03879324719309807,
0.013653990812599659,
-0.07120896875858307,
0.04696161672472954,
-0.0344465933740139,
-0.022749708965420723,
0.010646102949976921,
0.38005873560905457,
0.000998681876808405,
0.0031246000435203314,
-0.08467185497283936,
-0.059910282492637634,
-0.05381537228822708,
-0.0013184447307139635,
-0.025685472413897514,
-0.01397732924669981,
0.03984002023935318,
0.06262378394603729,
0.07020676136016846,
-0.013380033895373344,
0.06420569866895676,
0.00996212288737297,
-0.02288854494690895,
-0.02342805825173855,
-0.008209729567170143,
0.010556155815720558,
-0.0534745417535305,
0.02843509428203106,
-0.06713283807039261,
0.01547019649296999,
0.04351938143372536,
-0.03125111758708954,
0.05191868543624878,
-0.018055476248264313,
-0.018609927967190742,
0.06734441220760345,
0.03456629812717438,
-0.013811256736516953,
0.04020843654870987,
0.05604233592748642,
0.025261910632252693,
0.009870307520031929,
0.04522792994976044,
-0.029513003304600716,
0.053560055792331696,
-0.036829378455877304,
0.00024701195070520043,
0.009593096561729908,
-0.02560478262603283,
-0.030366934835910797,
-0.05880739539861679,
-0.06089995428919792,
-0.010300411842763424,
0.09515538066625595,
0.03113499842584133,
-0.026505904272198677,
-0.015235730446875095,
0.031182829290628433,
0.010405107401311398,
0.09279527515172958,
-0.0008534162770956755,
0.04998760670423508,
-0.0036289996933192015,
-0.030705399811267853,
-0.0029869843274354935,
0.003126132767647505,
-0.026255059987306595,
0.015983426943421364,
-0.043491046875715256,
-0.030234115198254585,
-0.011262628249824047,
0.0984506830573082,
0.01578829251229763,
-0.060377754271030426,
-0.01381759811192751,
0.054131560027599335,
-0.014055855572223663,
-0.025257349014282227,
-0.02192813716828823,
-0.05146003141999245,
-0.025221459567546844,
-0.026683993637561798,
-0.0009193589794449508,
0.06653734296560287,
0.04203948751091957,
-0.01548264641314745,
0.06401634216308594,
-0.012935415841639042,
0.03523918241262436,
-0.021371491253376007,
-0.036536671221256256,
0.012610970996320248,
0.05918155610561371,
-0.018963346257805824,
-0.08113276213407516,
0.027450554072856903,
0.04255132004618645,
0.04445013403892517,
-0.04961465299129486,
-0.013136753812432289,
-0.06873011589050293,
0.021433120593428612,
-0.04483838751912117,
-0.025906799361109734,
-0.028005383908748627,
0.009332248009741306,
-0.006985839921981096,
-0.03776577487587929,
0.07069842517375946,
0.028352530673146248,
0.050279393792152405,
0.024050164967775345,
0.015092352405190468,
0.14444197714328766,
-0.01662362925708294,
-0.04040031135082245,
0.019405491650104523,
-0.0008068706374615431,
-0.016396159306168556,
0.049154821783304214,
-0.025123372673988342,
0.0139787457883358,
-0.0637122318148613,
0.09818512946367264,
0.05002647265791893,
0.004155620466917753,
0.06867622584104538,
-0.014344730414450169,
0.0629429891705513,
-0.055392518639564514,
-0.03762086480855942,
-0.32913798093795776,
-0.05444110557436943,
0.0061576832085847855,
-0.013476439751684666,
0.09085018187761307,
-0.04970735311508179,
0.0353347510099411,
-0.05246720463037491,
0.07255235314369202,
-0.0658964291214943,
0.029340114444494247,
0.0450274683535099,
0.02003553695976734,
-0.03348873555660248,
0.00020909265731461346,
0.015470057725906372,
0.014570699073374271,
-0.028181904926896095,
0.05375862121582031,
0.04134958237409592,
0.014437975361943245,
-0.017179766669869423,
-0.0011455187341198325,
0.0798913836479187,
0.005612995009869337,
-0.027689939364790916,
0.07662256807088852,
0.006863703485578299,
-0.024318577721714973,
-0.011038536205887794,
-0.05045796185731888,
0.028390182182192802,
-0.013695266097784042,
-0.0861920714378357,
-0.0023375425953418016,
-0.024999644607305527,
0.004898598417639732,
-0.02421754226088524,
-0.004841306246817112,
-0.011297008022665977,
-0.0511164553463459,
0.04370824247598648,
-0.014415494166314602,
-0.04117506742477417,
-0.04149167612195015,
0.05454528331756592,
0.021182062104344368,
-0.0031599258072674274,
-0.016422748565673828,
0.024648142978549004,
0.0687917172908783,
0.0281451977789402,
0.0005843043909408152,
-0.01855381950736046,
-0.067982979118824,
-0.015095030888915062,
-0.01993655227124691,
0.017400627955794334,
-0.019054124131798744,
0.03877437487244606,
0.028111105784773827,
-0.00838389340788126,
-0.0013484129449352622,
-0.006196554750204086,
-0.015122483484447002,
-0.0060011292807757854,
0.0005795743782073259,
-0.03622548654675484,
0.02703242376446724,
0.016961362212896347,
0.0029293291736394167,
0.07271400094032288,
-0.030232012271881104,
0.03597700968384743,
0.012590024620294571,
-0.010975387878715992,
0.014764323830604553,
-0.0503864623606205,
-0.07460610568523407,
0.02052524872124195,
-0.01221243105828762,
-0.049518804997205734,
0.028872009366750717,
-0.0020790300332009792,
-0.00013156485510990024,
-0.013727220706641674,
-0.025144651532173157,
-0.027690334245562553,
-0.0030521119479089975,
0.02962171658873558,
0.024003876373171806,
0.013926170766353607,
0.01084141619503498,
0.04220949485898018,
0.0068976921029388905,
0.04195975139737129,
-0.2685687839984894,
-0.02695688232779503,
0.020247800275683403,
0.025516796857118607,
-0.039932213723659515,
0.036163099110126495,
0.03395829349756241,
-0.0354011170566082,
-0.01962209679186344,
0.08579830080270767,
-0.012261624448001385,
0.013580664992332458,
0.08948632329702377,
-0.0009357811068184674,
-0.052096616476774216,
-0.01837429776787758,
0.02815319411456585,
-0.05215929448604584,
-0.052530210465192795,
-0.002930605551227927,
-0.011425308883190155,
-0.02710145153105259,
0.11018958687782288,
-0.0036751774605363607,
-0.005657014902681112,
0.036132294684648514,
-0.015956809744238853,
0.02180624194443226,
-0.03241637349128723,
-0.013704545795917511,
-0.025052513927221298,
0.026350725442171097,
0.03170298784971237,
-0.010479290969669819,
0.02859424613416195,
-0.0023359577171504498,
-0.008876172825694084,
0.01700679585337639,
-0.019727809354662895,
-0.0070970249362289906,
-0.031246807426214218,
-0.01596076413989067,
-0.0991533100605011,
-0.04640473052859306,
0.03618960827589035,
-0.06670433282852173,
-0.044035185128450394,
-0.005536343436688185,
0.014812841080129147,
0.003496132791042328,
-0.031839825212955475,
-0.0675494372844696,
0.03535245731472969,
0.05570940673351288,
-0.03361353278160095,
-0.03218564763665199,
0.02496292255818844,
0.002971719251945615,
-0.056730709969997406,
0.028884530067443848,
0.029364224523305893,
-0.01784837618470192,
0.0004360557359177619,
0.045102592557668686,
0.0325857475399971
] |
PMID:8389 | The effect of prostaglandins F1 alpha and F2 alpha on spermatogenesis. | The effect of the prostaglandins F1 alpha and F2 alpha on spermatogenesis in the mature male mouse has been studied. Administration of prostaglandins F1 alpha and F2 alpha subcutaneously at a dose of 3 mg/kg once a day for 15 days produced a pronounced decrease in spermatogenesis primarily during the meiotic phase as reflected by a significant decrease in stage 7 spermatids as compared to controls. PGF2 alpha produced a stronger suppression than did PGF1 alpha. Accessory reproductive gland weights also appeared to be reduced by the prostaglandins, although not consistently. Histopathological examination revealed increased numbers of exfoliated immature germinal cells in the seminiferous tubules and epididymi of prostaglandin treated animals. When the effect of PGF1 alpha and PGF2 alpha were compared to those of PGE1 and PGE2 it was found that PGE2 produced the strongest suppression, with PGF2 alpha, PGE1 and PGF1 following in decreasing order respectively. | The effect of prostaglandins F1 alpha and F2 alpha on spermatogenesis. The effect of the prostaglandins F1 alpha and F2 alpha on spermatogenesis in the mature male mouse has been studied. Administration of prostaglandins F1 alpha and F2 alpha subcutaneously at a dose of 3 mg/kg once a day for 15 days produced a pronounced decrease in spermatogenesis primarily during the meiotic phase as reflected by a significant decrease in stage 7 spermatids as compared to controls. PGF2 alpha produced a stronger suppression than did PGF1 alpha. Accessory reproductive gland weights also appeared to be reduced by the prostaglandins, although not consistently. Histopathological examination revealed increased numbers of exfoliated immature germinal cells in the seminiferous tubules and epididymi of prostaglandin treated animals. When the effect of PGF1 alpha and PGF2 alpha were compared to those of PGE1 and PGE2 it was found that PGE2 produced the strongest suppression, with PGF2 alpha, PGE1 and PGF1 following in decreasing order respectively. | [
-0.0040074000135064125,
0.017216870561242104,
-0.0020291185937821865,
-0.037082165479660034,
-0.012610336765646935,
0.05023247376084328,
0.004812461324036121,
0.05238400399684906,
-0.030955549329519272,
-0.036296918988227844,
0.08149576187133789,
-0.0763116329908371,
0.035824280232191086,
0.021959537640213966,
-0.039351560175418854,
0.002573405159637332,
-0.03579356521368027,
-0.005522637162357569,
-0.12884265184402466,
-0.008112452924251556,
0.05321693420410156,
-0.01840180531144142,
0.002157251350581646,
-0.09357009083032608,
-0.006643439177423716,
0.0341314859688282,
-0.04488163813948631,
-0.004386421293020248,
-0.014950169250369072,
-0.1485140323638916,
0.054058533161878586,
-0.0011811242438852787,
0.0023131431080400944,
-0.0054590702056884766,
-0.010327734984457493,
-0.05376388505101204,
-0.04411407932639122,
-0.0020657561253756285,
-0.03874634951353073,
0.032657068222761154,
0.06676162034273148,
0.013535777106881142,
-0.020997514948248863,
0.031882576644420624,
0.021217403933405876,
0.037640560418367386,
-0.04782215878367424,
-0.020096568390727043,
0.07696583867073059,
0.010899335145950317,
0.10286223143339157,
-0.012376919388771057,
0.010039049200713634,
0.029717080295085907,
-0.005911678541451693,
-0.030446890741586685,
0.014172524213790894,
0.04097225144505501,
-0.039986055344343185,
0.0716426894068718,
-0.02971581742167473,
-0.017766334116458893,
-0.16606086492538452,
0.09554550796747208,
0.04592878371477127,
-0.0008720916812308133,
0.00901580136269331,
-0.03488374128937721,
0.0441027507185936,
0.06851857900619507,
-0.054432712495326996,
0.10445014387369156,
0.0013464582152664661,
-0.023460621014237404,
0.005159242078661919,
-0.02130177430808544,
-0.03329754248261452,
-0.027316568419337273,
0.05967047065496445,
-0.01925848424434662,
-0.014682527631521225,
-0.02775598131120205,
-0.028078364208340645,
-0.02672582119703293,
-0.0715346410870552,
-0.05616641789674759,
0.007977793924510479,
-0.04646691679954529,
0.03609927371144295,
0.021423300728201866,
0.03433661907911301,
-0.04481508582830429,
-0.03993196040391922,
-0.03667910024523735,
0.005093323532491922,
0.06669966131448746,
-0.014629147946834564,
-0.024746166542172432,
0.02101605013012886,
0.3410331606864929,
-0.04856822267174721,
0.02872448042035103,
-0.0802261009812355,
-0.01996544376015663,
0.005457714200019836,
-0.02527639828622341,
-0.0370502732694149,
0.015201336704194546,
-0.009915423579514027,
0.04596082866191864,
0.04700498655438423,
0.0024218654725700617,
0.0902734026312828,
-0.01910608634352684,
-0.02985280007123947,
-0.053557559847831726,
0.0811016783118248,
0.0053842943161726,
-0.05683032423257828,
0.07438360899686813,
-0.0034610810689628124,
0.0037314295768737793,
0.026476018130779266,
-0.009178134612739086,
0.05995262786746025,
-0.0037944563664495945,
0.029192620888352394,
0.037765320390462875,
-0.015780622139573097,
-0.01639196090400219,
0.061902910470962524,
0.0669998750090599,
-0.003370741382241249,
0.043198104947805405,
0.001355259446427226,
-0.031973596662282944,
-0.005091994069516659,
-0.01711190864443779,
-0.022179365158081055,
0.04443800821900368,
0.028904015198349953,
-0.06064155697822571,
-0.04508328065276146,
-0.06509558111429214,
0.02157435193657875,
0.13310502469539642,
0.06368140131235123,
0.006706596817821264,
-0.04875948652625084,
0.04373497888445854,
0.03133071959018707,
0.05937425047159195,
0.02489968203008175,
0.01860954612493515,
0.013477371074259281,
-0.0010512889130041003,
0.0004466156824491918,
0.005989147815853357,
-0.014341091737151146,
-0.008451678790152073,
-0.11123230308294296,
0.03826414421200752,
-0.01293263491243124,
0.01788308285176754,
0.04246322065591812,
-0.01716342382133007,
-0.056848473846912384,
0.027704810723662376,
-0.02027473784983158,
0.012238478288054466,
-0.04528649523854256,
-0.0006828811019659042,
-0.037574708461761475,
-0.06551524996757507,
-0.006877055857330561,
-0.020047374069690704,
0.004898824729025364,
-0.00030369465821422637,
0.08849237859249115,
0.019906263798475266,
0.05945330485701561,
-0.053606096655130386,
0.007694638799875975,
-0.00207728729583323,
-0.023677030578255653,
-0.03522268682718277,
-0.08682604879140854,
0.06402730196714401,
0.04158276319503784,
0.04291977733373642,
-0.028462639078497887,
-0.0018381769768893719,
-0.03811861202120781,
-0.00482754223048687,
-0.01967216096818447,
-0.053895361721515656,
-0.02954668179154396,
-0.04250036180019379,
-0.05530109629034996,
-0.06938246637582779,
0.11959268152713776,
-0.011019849218428135,
0.056901026517152786,
0.04280873015522957,
-0.04179825633764267,
0.09649784117937088,
0.029757322743535042,
0.023073675110936165,
0.056284099817276,
-0.005728025455027819,
-0.03795599564909935,
-0.023810993880033493,
0.000731608597561717,
0.01764894835650921,
-0.0638500303030014,
0.11086251586675644,
-0.017896661534905434,
-0.008511490188539028,
0.05775585025548935,
0.012034568935632706,
0.07109981030225754,
0.0006319552776403725,
-0.006977335549890995,
-0.31633588671684265,
-0.04660039767622948,
-0.0036739010829478502,
-0.02666078321635723,
0.008124603889882565,
-0.005947329103946686,
0.008656526915729046,
-0.039979416877031326,
0.013822480104863644,
-0.02571222186088562,
0.042352933436632156,
0.07592207193374634,
-0.034485649317502975,
0.03418014198541641,
0.001013773726299405,
-0.002527428325265646,
0.019173284992575645,
-0.06642305850982666,
0.034554630517959595,
0.016758384183049202,
-0.05255463346838951,
-0.034756146371364594,
-0.05875413492321968,
0.041781388223171234,
0.08939658105373383,
0.0037486758083105087,
0.08372035622596741,
-0.011528297327458858,
-0.037803277373313904,
-0.08192583173513412,
0.06099191680550575,
0.015247737057507038,
0.026219971477985382,
-0.05033564940094948,
0.023161504417657852,
-0.000607259338721633,
0.005973722320050001,
-0.008906254544854164,
-0.012190666981041431,
0.010072301141917706,
-0.02007652446627617,
0.03492589667439461,
0.015284066088497639,
-0.02228558622300625,
-0.08028439432382584,
-0.048268962651491165,
0.05858268216252327,
0.00705177616328001,
-0.019906291738152504,
-0.006209652870893478,
0.03560309857130051,
0.03438068553805351,
0.0050979312509298325,
-0.07059089094400406,
-0.012205946259200573,
-0.031238233670592308,
-0.04205654561519623,
0.03440301865339279,
-0.06630443036556244,
-0.018798047676682472,
0.05645626038312912,
0.019526634365320206,
0.043853674083948135,
-0.06577486544847488,
-0.00039558843127451837,
-0.04496671259403229,
-0.0072011700831353664,
-0.01998463273048401,
-0.0016352329403162003,
0.034301888197660446,
0.04139142856001854,
0.07957858592271805,
-0.07961613684892654,
-0.014478863216936588,
0.0524907186627388,
0.0065894583240151405,
0.009701063856482506,
-0.03452383354306221,
-0.07154674828052521,
-0.01686144433915615,
0.013558355160057545,
-0.017603425309062004,
0.028409451246261597,
-0.06184070184826851,
-0.0254033375531435,
-0.018029307946562767,
-0.03072080761194229,
-0.027395574375987053,
0.012312520295381546,
-0.021189548075199127,
0.013715365901589394,
0.0023800134658813477,
-0.02389349788427353,
0.008520614355802536,
0.01882234588265419,
0.030701756477355957,
-0.25341516733169556,
-0.05446165055036545,
0.107871413230896,
-0.006303954869508743,
-0.03656778112053871,
0.06386317312717438,
0.016082629561424255,
-0.02763824164867401,
-0.02173542231321335,
0.008122573606669903,
0.02840230241417885,
0.02502676285803318,
0.09623166173696518,
0.02140127122402191,
-0.05090375989675522,
0.018148774281144142,
0.010436671786010265,
0.008920702151954174,
0.0012480890145525336,
-0.047058213502168655,
0.02284647524356842,
-0.05993116274476051,
0.12154583632946014,
0.0031280440744012594,
0.018484771251678467,
0.023585345596075058,
0.03319096192717552,
0.03729330748319626,
0.02186807058751583,
0.02208169549703598,
-0.010580802336335182,
0.011836095713078976,
0.024102624505758286,
-0.035120029002428055,
-0.015156997367739677,
0.018093911930918694,
-0.04257175326347351,
-0.010292881168425083,
0.0057687945663928986,
0.0026744059287011623,
0.0071655092760920525,
-0.024001415818929672,
-0.022223202511668205,
0.017567681148648262,
0.034614648669958115,
-0.08060324192047119,
-0.031301259994506836,
0.020754363387823105,
0.027163883671164513,
0.028431516140699387,
-0.033501897007226944,
-0.020854463800787926,
0.031097790226340294,
0.05510324984788895,
-0.008658148348331451,
-0.04876700043678284,
0.02201821655035019,
0.014177624136209488,
0.035339128226041794,
0.07564936578273773,
0.02290191315114498,
0.0028523742221295834,
-0.043450385332107544,
0.08800513297319412,
0.027644827961921692
] |
PMID:8390 | Immunological factors and post coital test in unexplained infertility. | Twenty-four couples facing longstanding primary or secondary infertility underwent semen analysis, PCT, functional evaluation of menstrual cycle, hysterosalpingography and laparscopy. All clinical findings were normal with the exception of PCT which was positive in 13 and negative in 11. All the women underwent a multiple approach investigation of local and circulating antibodies production as well as cell-mediated immunity against live spermatozoa. Sperm Immobilization Test (SIT) and Spermatotoxicity Tests (STT) were performed in a single experimental design on cervical mucus and blood serum. Leucocyte Migration Inhibition Test in presence of sperms (LMIT) was done on peripheral leucocytes. Local antispermatic activity in the cervical mucus was negative in all PCT positive cases, and positive in six out of 11 PCT negative cases. SIT and STT were both positive in cervical mucus and in blood in one case only. No correlation could be found between PCT and serum SIT, STT or LMIT. The fertility pattern expressed by the number of pregnancies per years of exposure, already low in the whole group, was even lower in the sub-groups with positive immunological factors. Positive immunological factors do not exclude the possibility of conception but appear to be associated with a reduced rate of conception. | Immunological factors and post coital test in unexplained infertility. Twenty-four couples facing longstanding primary or secondary infertility underwent semen analysis, PCT, functional evaluation of menstrual cycle, hysterosalpingography and laparscopy. All clinical findings were normal with the exception of PCT which was positive in 13 and negative in 11. All the women underwent a multiple approach investigation of local and circulating antibodies production as well as cell-mediated immunity against live spermatozoa. Sperm Immobilization Test (SIT) and Spermatotoxicity Tests (STT) were performed in a single experimental design on cervical mucus and blood serum. Leucocyte Migration Inhibition Test in presence of sperms (LMIT) was done on peripheral leucocytes. Local antispermatic activity in the cervical mucus was negative in all PCT positive cases, and positive in six out of 11 PCT negative cases. SIT and STT were both positive in cervical mucus and in blood in one case only. No correlation could be found between PCT and serum SIT, STT or LMIT. The fertility pattern expressed by the number of pregnancies per years of exposure, already low in the whole group, was even lower in the sub-groups with positive immunological factors. Positive immunological factors do not exclude the possibility of conception but appear to be associated with a reduced rate of conception. | [
0.024680983275175095,
0.07296927273273468,
-0.021685102954506874,
0.005650943145155907,
0.0021491979714483023,
0.009571305476129055,
0.1003754734992981,
0.04691552370786667,
0.013883573934435844,
-0.01048314943909645,
0.018171411007642746,
-0.1040087565779686,
0.002031581476330757,
0.028997143730521202,
-0.04795130714774132,
-0.003412711201235652,
0.0014001121744513512,
-0.030788766220211983,
-0.051950179040431976,
0.04441971331834793,
0.007395892404019833,
-0.010045635513961315,
-0.027247704565525055,
-0.061291053891181946,
0.0622088722884655,
0.016588952392339706,
-0.02252708189189434,
-0.010973149910569191,
-0.02113419957458973,
-0.1414182186126709,
-0.028848350048065186,
-0.04409003630280495,
-0.031275246292352676,
0.009936012327671051,
0.0012219779891893268,
-0.014080916531383991,
-0.04767955094575882,
-0.006688357330858707,
-0.0469651073217392,
0.0266132690012455,
0.04594361409544945,
0.009093631990253925,
-0.015825305134058,
0.035282764583826065,
0.00780849065631628,
-0.013711158186197281,
-0.02274150401353836,
-0.008384154178202152,
0.03781439736485481,
0.009114832617342472,
0.03172602877020836,
0.0017200487200170755,
0.019968772307038307,
0.09256889671087265,
-0.05867509916424751,
-0.03207417577505112,
-0.03990355134010315,
0.03697347268462181,
-0.0360494963824749,
-0.010283112525939941,
-0.04303731769323349,
0.05575483292341232,
-0.1460215002298355,
0.07638851553201675,
0.08244645595550537,
-0.03647496923804283,
0.0066139851696789265,
-0.04165845364332199,
0.06077556312084198,
0.006116373930126429,
0.03836892172694206,
0.05822150781750679,
-0.025368012487888336,
0.052060045301914215,
0.02067541517317295,
-0.019937189295887947,
-0.008206366561353207,
-0.034179575741291046,
0.008580954745411873,
0.014104573987424374,
0.0020088176243007183,
0.05027834326028824,
0.032929934561252594,
0.028839614242315292,
-0.036384422332048416,
-0.033605728298425674,
-0.02970246411859989,
-0.01640954241156578,
0.026467885822057724,
0.007081482093781233,
0.01820286363363266,
-0.045000750571489334,
-0.051058124750852585,
0.01904730685055256,
-0.0746769905090332,
0.046648457646369934,
0.004698663018643856,
-0.0169850904494524,
0.012330809608101845,
0.4398593306541443,
-0.07130910456180573,
0.01882711425423622,
-0.08450233191251755,
0.04899602755904198,
-0.018768534064292908,
-0.014189600013196468,
0.0048592789098620415,
-0.039819348603487015,
-0.022729547694325447,
0.027061685919761658,
-0.005856429226696491,
-0.00457383319735527,
0.05906303599476814,
-0.05511623993515968,
0.011714923195540905,
-0.006107048597186804,
0.07621709257364273,
0.02942277118563652,
-0.0038316743448376656,
0.03639061376452446,
-0.002577267587184906,
-0.03213115409016609,
0.02638210915029049,
-0.07637342065572739,
0.05727420747280121,
-0.04548945277929306,
0.049941204488277435,
0.05982043966650963,
0.0016738696722313762,
0.01992056332528591,
0.0670924037694931,
0.047586891800165176,
-0.027471857145428658,
0.0369139090180397,
0.0014086180599406362,
0.0010931693250313401,
0.023217538371682167,
0.033125799149274826,
0.011050406843423843,
-0.01190097164362669,
0.02275269292294979,
-0.06757549196481705,
-0.017981529235839844,
-0.09691257029771805,
0.03611390292644501,
0.06058289110660553,
0.024730108678340912,
0.01026119850575924,
0.007296361029148102,
-0.004298651125282049,
0.03848518058657646,
0.06580466777086258,
-0.05109773203730583,
0.006050787400454283,
0.008455315604805946,
-0.017778607085347176,
-0.047173213213682175,
0.008671854622662067,
-0.02886081300675869,
-0.01144714467227459,
-0.03845980763435364,
-0.016911227256059647,
-0.05415503308176994,
0.031714387238025665,
0.007507954258471727,
-0.037487126886844635,
-0.022921469062566757,
0.0033918183762580156,
0.011095324531197548,
-0.03104877844452858,
0.032158106565475464,
-0.03291518613696098,
-0.04424570873379707,
-0.023486891761422157,
-0.02923646755516529,
0.0019825841300189495,
-0.053237684071063995,
0.10628446191549301,
0.014395487494766712,
0.053462106734514236,
-0.011704104952514172,
-0.045614272356033325,
-0.02076657861471176,
0.020105494186282158,
-0.009846949018537998,
-0.03919451683759689,
-0.05716075748205185,
0.018113501369953156,
0.07019130885601044,
-0.03542337939143181,
0.06628783047199249,
-0.03159884735941887,
-0.11165164411067963,
0.050834786146879196,
0.007778265979140997,
0.003550522727891803,
-0.046157605946063995,
-0.023316435515880585,
0.0182462390512228,
-0.06322920322418213,
0.09255284070968628,
-0.020403940230607986,
0.047427453100681305,
0.06185683235526085,
0.015073197893798351,
0.038921426981687546,
0.0413675382733345,
-0.05264727771282196,
0.08078277111053467,
0.02764435112476349,
-0.031213946640491486,
-0.007776849903166294,
-0.020756259560585022,
0.017618328332901,
-0.014150259084999561,
0.014307734556496143,
-0.007632382679730654,
-0.04617151618003845,
0.06531820446252823,
0.02389160543680191,
0.037581611424684525,
0.04491202160716057,
0.03618032857775688,
-0.31712988018989563,
-0.0663704052567482,
-0.00212973915040493,
0.024723710492253304,
0.006463734898716211,
-0.02882233075797558,
-0.03018285520374775,
0.025038428604602814,
0.065581314265728,
-0.022954750806093216,
0.09229094535112381,
0.10456712543964386,
-0.08515693247318268,
-0.009012332186102867,
0.03166493400931358,
-0.011557789519429207,
0.025970282033085823,
-0.0720231756567955,
-0.023507852107286453,
0.01049891859292984,
-0.04415164515376091,
0.010938008315861225,
-0.0035823453217744827,
0.036748431622982025,
0.06087388098239899,
-0.020723333582282066,
0.11559686064720154,
-0.011004088446497917,
-0.03046087920665741,
-0.008847585879266262,
0.03727324306964874,
0.03732586279511452,
0.030822841450572014,
-0.02936656028032303,
-0.00730513921007514,
-0.01774543710052967,
-0.01960141398012638,
0.004712775815278292,
-0.039304617792367935,
0.033793769776821136,
-0.039178553968667984,
0.010789935477077961,
-0.029831407591700554,
-0.006048333831131458,
-0.02743549272418022,
0.04694058373570442,
0.0326034277677536,
0.0831230953335762,
0.022692779079079628,
0.02746182680130005,
0.01981738768517971,
-0.038236770778894424,
0.013534818775951862,
0.001680338871665299,
-0.037802036851644516,
0.02647852525115013,
-0.06528628617525101,
-0.008658239617943764,
-0.06079087406396866,
-0.0011027937289327383,
0.03621814027428627,
0.027945037931203842,
0.025901436805725098,
-0.022060874849557877,
-0.036768075078725815,
0.0017711356049403548,
0.011471518315374851,
-0.013540896587073803,
0.05315128713846207,
-0.005435660947114229,
0.013777194544672966,
0.11063168942928314,
-0.020500963553786278,
0.02984095737338066,
-0.004592029377818108,
-0.001521592726930976,
0.030766841024160385,
0.021322468295693398,
-0.07029186934232712,
-0.012461167760193348,
0.027388427406549454,
0.011764820665121078,
-0.00104798364918679,
-0.039938200265169144,
0.004988623317331076,
0.020499849691987038,
-0.0028642762918025255,
-0.03699444234371185,
0.001169060473330319,
0.009220384061336517,
-0.0016337434062734246,
-0.006117655895650387,
-0.013513092882931232,
-0.009650991298258305,
-0.028110910207033157,
-0.012089976109564304,
-0.2679608166217804,
-0.02265886217355728,
-0.009674911387264729,
-0.010178468190133572,
-0.08459805697202682,
-0.0030182322952896357,
-0.019576359540224075,
-0.012079360894858837,
-0.03408537060022354,
0.05468280613422394,
0.06046774983406067,
0.010601821355521679,
0.05269981548190117,
-0.020208163186907768,
-0.04430709779262543,
0.08027133345603943,
0.009706641547381878,
-0.015386979095637798,
-0.02243911102414131,
-0.04385019466280937,
0.04119013622403145,
-0.01779770478606224,
0.13571925461292267,
0.0015862681902945042,
0.0012161631602793932,
0.015476018190383911,
0.05262215808033943,
0.02275848761200905,
-0.03511752188205719,
-0.05757787823677063,
-0.0064262752421200275,
-0.021557169035077095,
0.020256901159882545,
-0.006030038930475712,
-0.011937927454710007,
-0.04834873601794243,
-0.018314193934202194,
-0.014920338988304138,
0.012671523727476597,
0.00010511442087590694,
0.003298424882814288,
0.02859443984925747,
-0.043255988508462906,
-0.07839252799749374,
0.03685556352138519,
-0.044941943138837814,
-0.01441198494285345,
-0.06535210460424423,
0.046252358704805374,
-0.008761374279856682,
-0.025936927646398544,
-0.05225653946399689,
0.007054472807794809,
0.0019604507833719254,
-0.009612432681024075,
-0.0434572659432888,
0.031259696930646896,
-0.039619330316782,
0.00656828423961997,
0.051840804517269135,
0.03226665034890175,
0.009516216814517975,
-0.00565178832039237,
0.09288103133440018,
0.014890721999108791
] |
PMID:8391 | Pituitary and testicular response to hypothalamic LH-releasing hormone (LH-RH) in normal and oligospermic men. | A comparative study was done of the basal and post-LH-RH stimulation levels of LH, FSH and Testosterone (T) in 10 healthy, male volunteers, aged 24-35 years, and in 25 normogonadotropic oligospermic men aged 23-43 years in whom other endocrine, urologic and/or vascular diseases had been rules out. Seventeen of the latter group underwent bilateral testicular biopsy. Each subject received rapid i.v. injection of 50 mug synthetic LH-RH at 8:00 A.M. Serum levels of LH, FSH and T were determined by radioimmunoassay on samples obtained immediately before and 30 and 45 minutes after injection. As compared to normals, oligospermic men had a lower average LH response, although the basal values did not differ significantly from normal. FSH levels did not differ significantly between groups, although some individual patients showed higher and some lower than normal values. The possibility of stimulating T secretion with LH-RH was confirmed. Average serum T levels rose more slowly and reached lower levels in the oligospermic men than in normal men. There was no correlation between LH and FSH rises. It is postulated that some patients with normogonadotropic oligospermia may have latent insufficiency of pituitary gonadotropic functional reserve for one or both hormones and/or of the Leydig cell function. The need for individual analysis of results and their correlation with testicular biopsy findings is commented. | Pituitary and testicular response to hypothalamic LH-releasing hormone (LH-RH) in normal and oligospermic men. A comparative study was done of the basal and post-LH-RH stimulation levels of LH, FSH and Testosterone (T) in 10 healthy, male volunteers, aged 24-35 years, and in 25 normogonadotropic oligospermic men aged 23-43 years in whom other endocrine, urologic and/or vascular diseases had been rules out. Seventeen of the latter group underwent bilateral testicular biopsy. Each subject received rapid i.v. injection of 50 mug synthetic LH-RH at 8:00 A.M. Serum levels of LH, FSH and T were determined by radioimmunoassay on samples obtained immediately before and 30 and 45 minutes after injection. As compared to normals, oligospermic men had a lower average LH response, although the basal values did not differ significantly from normal. FSH levels did not differ significantly between groups, although some individual patients showed higher and some lower than normal values. The possibility of stimulating T secretion with LH-RH was confirmed. Average serum T levels rose more slowly and reached lower levels in the oligospermic men than in normal men. There was no correlation between LH and FSH rises. It is postulated that some patients with normogonadotropic oligospermia may have latent insufficiency of pituitary gonadotropic functional reserve for one or both hormones and/or of the Leydig cell function. The need for individual analysis of results and their correlation with testicular biopsy findings is commented. | [
0.07105524837970734,
0.03942364826798439,
-0.010368578135967255,
0.014777513220906258,
0.015642337501049042,
0.0007316641276702285,
0.04965457320213318,
0.038269802927970886,
-0.01828649267554283,
-0.028463441878557205,
0.0034326647873967886,
-0.05319109559059143,
0.03726206719875336,
0.06428666412830353,
-0.0032709913793951273,
0.04562041908502579,
0.0333588533103466,
-0.04093688726425171,
-0.06594488024711609,
0.045299191027879715,
0.04513602703809738,
-0.010514871217310429,
0.02867458201944828,
0.011644784361124039,
-0.014660954475402832,
0.018849801272153854,
-0.054476238787174225,
-0.015163890086114407,
-0.03390100225806236,
-0.14667434990406036,
0.05324072018265724,
-0.026573872193694115,
0.02971765398979187,
-0.024214794859290123,
0.0030482090078294277,
-0.0329323373734951,
-0.03636927530169487,
-0.02367585338652134,
0.008228817023336887,
0.012078995816409588,
0.02511102706193924,
-0.03693842887878418,
-0.000657504890114069,
0.027481650933623314,
0.01399375032633543,
0.08911168575286865,
-0.06815480440855026,
0.046146612614393234,
0.03700873255729675,
0.022552648559212685,
-0.019556794315576553,
0.01595924235880375,
0.049569517374038696,
0.07355794310569763,
-0.017584795132279396,
-0.035959675908088684,
0.02378557063639164,
0.01692192442715168,
-0.01905142515897751,
0.018719539046287537,
-0.03743808716535568,
0.060882702469825745,
-0.135282501578331,
0.03678220137953758,
0.08160150051116943,
0.02707848884165287,
-0.0299520380795002,
-0.02194671891629696,
0.0461917370557785,
0.007692596409469843,
-0.002880578860640526,
0.03255210071802139,
0.04621897637844086,
-0.026957061141729355,
-0.00762983737513423,
-0.061194173991680145,
-0.04053622484207153,
-0.04470054805278778,
0.017663443461060524,
0.003225654596462846,
0.026781437918543816,
0.0749422013759613,
0.016819266602396965,
-0.011653373949229717,
-0.04724438115954399,
-0.050249285995960236,
-0.006580054759979248,
-0.056064728647470474,
0.010089006274938583,
0.0583619587123394,
-0.03644426912069321,
-0.06495308130979538,
-0.03921965882182121,
0.02061832882463932,
-0.044907402247190475,
0.020242100581526756,
0.007853495888411999,
-0.07668822258710861,
-0.010953648947179317,
0.4250766634941101,
-0.06628552824258804,
-0.011461823247373104,
-0.08556319028139114,
-0.02348281629383564,
0.01509005855768919,
-0.00912565365433693,
-0.010587207041680813,
-0.008304317481815815,
-0.04474744573235512,
0.08171714097261429,
-0.0041992333717644215,
-0.03801530599594116,
0.07841330766677856,
-0.04972294345498085,
-0.05569281429052353,
-0.03802782669663429,
0.029369458556175232,
0.05395713821053505,
-0.03564511239528656,
0.007135821972042322,
0.0035643992014229298,
0.03236590698361397,
0.04183393716812134,
-0.09014677256345749,
0.029668880626559258,
-0.009925786405801773,
0.0659554973244667,
0.09194690734148026,
0.02735999971628189,
0.024874936789274216,
0.004250194877386093,
0.03706372529268265,
-0.05072791874408722,
0.024797087535262108,
-0.030825207009911537,
0.026692701503634453,
0.08169330656528473,
-0.02435910888016224,
0.006819840520620346,
0.0802864283323288,
-0.011839857324957848,
-0.11035037040710449,
-0.03821691498160362,
-0.059878647327423096,
-0.01852106861770153,
0.09761524200439453,
-0.030668798834085464,
-0.0050659277476370335,
-0.030390499159693718,
0.02651081047952175,
0.04853231832385063,
0.06529658287763596,
-0.046311575919389725,
0.0007394000422209501,
-0.006273649167269468,
0.020806467160582542,
-0.004084802232682705,
-0.004292343743145466,
-0.023683834820985794,
0.004185622092336416,
-0.08034404367208481,
-0.004689506255090237,
-0.017008544877171516,
-0.015518074855208397,
0.03741252049803734,
-0.021455856040120125,
-0.04757289960980415,
-0.007557441480457783,
0.0050439126789569855,
-0.041076451539993286,
0.02859201468527317,
-0.009272880852222443,
0.0030618426389992237,
0.0350969135761261,
-0.03422681614756584,
0.003466879716143012,
0.01504483725875616,
-0.03616204857826233,
0.03333143889904022,
0.03957262262701988,
0.01508725993335247,
-0.01943521946668625,
-0.038383401930332184,
-0.005443557631224394,
-0.01893303170800209,
0.004047650843858719,
-0.08122566342353821,
0.054977379739284515,
0.05173008143901825,
0.0080416239798069,
-0.02302739769220352,
-0.006948074325919151,
-0.08964503556489944,
-0.017549682408571243,
0.004224495962262154,
-0.030836546793580055,
-0.030674494802951813,
0.035977259278297424,
0.022494638338685036,
0.03612259775400162,
0.05081258714199066,
0.007595724891871214,
0.05486341938376427,
0.0067922333255410194,
-0.04365627095103264,
0.03912054002285004,
0.029035549610853195,
-0.027472784742712975,
0.08695503324270248,
0.047931089997291565,
-0.02548474632203579,
-0.026189574971795082,
-0.0007431346457451582,
0.03739432245492935,
-0.02784929797053337,
0.06235068663954735,
-0.02273757942020893,
-0.04205884411931038,
0.009324321523308754,
-0.01229409035295248,
0.020661214366555214,
0.013645379804074764,
0.03725077584385872,
-0.30355703830718994,
-0.06429997086524963,
0.012347372248768806,
0.003097412409260869,
0.046224337071180344,
-0.07362448424100876,
0.0055230665020644665,
0.010403076186776161,
0.030526909977197647,
-0.0034575366880744696,
0.06246126443147659,
0.0868956670165062,
-0.047646164894104004,
-0.031250931322574615,
-0.013300320133566856,
-0.007291118614375591,
0.04807146638631821,
-0.06951680034399033,
0.010001160204410553,
0.025728007778525352,
-0.019400114193558693,
0.023781271651387215,
-0.02239607460796833,
0.009883454069495201,
0.062747523188591,
-0.050410185009241104,
0.11454793065786362,
-0.03546705096960068,
-0.03978602588176727,
-0.02969355136156082,
0.04520222544670105,
0.03694813326001167,
0.057335883378982544,
-0.04617425054311752,
0.009171622805297375,
-0.027469094842672348,
-0.023829901590943336,
-0.029100950807332993,
-0.018422245979309082,
0.0041494220495224,
-0.06650551408529282,
-0.02564365044236183,
0.010890317149460316,
-0.04519611597061157,
-0.08592452108860016,
-0.01571943424642086,
0.008286537602543831,
-0.006317875813692808,
-0.029218148440122604,
-0.04579765722155571,
0.04636150225996971,
-0.05950664356350899,
0.07357507944107056,
-0.013687241822481155,
-0.036523740738630295,
-0.04572322219610214,
-0.11139421164989471,
-0.04642240330576897,
-0.04365896061062813,
0.016406958922743797,
0.04806481674313545,
0.025415711104869843,
0.04065997898578644,
-0.01750132627785206,
-0.03362133353948593,
-0.020906250923871994,
0.014184953644871712,
0.02429964579641819,
0.030922602862119675,
0.0026092843618243933,
0.016894780099391937,
0.03766728192567825,
-0.05151309818029404,
0.10250246524810791,
-0.00607762485742569,
-0.003832136048004031,
-0.03856828063726425,
0.02230902574956417,
-0.04097184166312218,
0.006756914779543877,
0.028479522094130516,
-0.017865927889943123,
0.0426466166973114,
0.023509610444307327,
0.008857485838234425,
-0.022266043350100517,
0.007318528369069099,
0.061748579144477844,
0.04066858068108559,
0.028161857277154922,
-0.04210464283823967,
0.014795174822211266,
0.03434869647026062,
-0.08520445972681046,
-0.025907889008522034,
0.014203360304236412,
-0.28516685962677,
-0.013296624645590782,
0.021989041939377785,
-0.0013451200211420655,
0.001808345434255898,
0.060809142887592316,
0.034223735332489014,
-0.0022682356648147106,
-0.04075932502746582,
0.06069079041481018,
0.01470036432147026,
0.05002937093377113,
0.07396228611469269,
0.03209396451711655,
-0.05786201357841492,
-0.006689524743705988,
-0.011701156385242939,
0.009464525617659092,
-0.03628939390182495,
-0.04853111878037453,
0.021634003147482872,
0.008798603899776936,
0.11504977196455002,
0.010050786659121513,
0.019145330414175987,
0.023834774270653725,
0.06363759934902191,
0.03437327221035957,
-0.01146603375673294,
-0.019747324287891388,
-0.0029984144493937492,
0.06487273424863815,
-0.019687630236148834,
-0.008970703929662704,
0.030338913202285767,
0.02057558111846447,
-0.008214873261749744,
0.027380777522921562,
0.03625334054231644,
-0.006646465510129929,
-0.026342710480093956,
0.013045337051153183,
-0.03332379087805748,
-0.0006984343053773046,
0.038110386580228806,
-0.050844110548496246,
-0.009328747168183327,
-0.01933411695063114,
0.0007576732314191759,
0.01916281133890152,
-0.0066871377639472485,
-0.03376554325222969,
0.022181019186973572,
0.06596697121858597,
0.0015140348114073277,
-0.021465249359607697,
0.006343163084238768,
-0.06618492305278778,
-0.029540620744228363,
0.01099548302590847,
-0.018727868795394897,
-0.02350989729166031,
-0.054588139057159424,
0.07517067342996597,
-0.010480917058885098
] |
PMID:8394 | Alkaline phosphatase, arylsulfatase and beta-glucuronidase in saliva of cyclic women. | Levels of the enzymes alkaline phosphatase, arylsulfatase, and beta-glucuronidase have been measured in whole unstimulated saliva samples collected daily during normal menstrual cycles. The cellular content of the saliva has been shown to be a major source of the three enzymes. The enzymes were found to have peak maximum levels of activity during the periovulatory -hase of the cycle. This observation can be attributed to the presence of increased numbers of exfoliated cells from the oral mucosa resulting from the pre-ovulatory estrogen stimulus to cell proliferation. | Alkaline phosphatase, arylsulfatase and beta-glucuronidase in saliva of cyclic women. Levels of the enzymes alkaline phosphatase, arylsulfatase, and beta-glucuronidase have been measured in whole unstimulated saliva samples collected daily during normal menstrual cycles. The cellular content of the saliva has been shown to be a major source of the three enzymes. The enzymes were found to have peak maximum levels of activity during the periovulatory -hase of the cycle. This observation can be attributed to the presence of increased numbers of exfoliated cells from the oral mucosa resulting from the pre-ovulatory estrogen stimulus to cell proliferation. | [
0.007890700362622738,
0.02278343215584755,
-0.05502941459417343,
0.02780112996697426,
0.0044598956592381,
-0.054165106266736984,
0.03901330754160881,
0.00872476864606142,
0.018545398488640785,
-0.05298297107219696,
-0.023317871615290642,
-0.10390183329582214,
-0.0077379681169986725,
0.01748977042734623,
0.012434916570782661,
-0.04214300960302353,
0.02397543005645275,
-0.007307072635740042,
0.012754304334521294,
-0.0018709746655076742,
0.04549875855445862,
0.013118897564709187,
0.04068349301815033,
-0.017912564799189568,
0.02807297557592392,
0.035331133753061295,
-0.009173338301479816,
-0.0005289012915454805,
-0.037827033549547195,
-0.1462942659854889,
-0.051794182509183884,
-0.06295192241668701,
-0.009862026199698448,
-0.03699169680476189,
-0.03355534374713898,
0.038952428847551346,
-0.0315471850335598,
0.05588860437273979,
0.02297278866171837,
0.04965794086456299,
0.008811953477561474,
0.003580240998417139,
-0.055305834859609604,
-0.009756465442478657,
0.015403016470372677,
0.0396021269261837,
-0.08144031465053558,
0.05818520858883858,
0.05305732786655426,
0.010638452135026455,
0.021958624944090843,
0.004101149272173643,
0.032260578125715256,
0.018727445974946022,
-0.019772866740822792,
-0.0015515447594225407,
0.039258576929569244,
0.05993342027068138,
-0.0019558221101760864,
0.05121482536196709,
-0.008508901111781597,
-0.03934616222977638,
-0.1543186753988266,
0.060805272310972214,
0.046250972896814346,
0.0004273987142369151,
0.024672145023941994,
-0.016206126660108566,
0.023434147238731384,
0.06417980790138245,
-0.06466411054134369,
0.061338428407907486,
0.02377082221210003,
-0.010166225023567677,
0.02884431555867195,
-0.005959028378129005,
-0.006513981148600578,
0.015198045410215855,
-0.009925498627126217,
0.05158425495028496,
0.012956180609762669,
-0.0008147727348841727,
0.03570141643285751,
0.010734038427472115,
0.010635331273078918,
-0.03600747510790825,
0.002816401422023773,
-0.015797751024365425,
0.02646569348871708,
-0.0030685565434396267,
-0.07480805367231369,
-0.04498451203107834,
-0.036466117948293686,
-0.005448261741548777,
-0.02509903907775879,
0.03548665717244148,
0.05283421650528908,
0.030683621764183044,
-0.00017193912935908884,
0.388608455657959,
-0.0670713260769844,
-0.01601487398147583,
-0.0674440860748291,
-0.01640593446791172,
0.0103763984516263,
-0.010755201801657677,
-0.030685564503073692,
-0.03661084175109863,
-0.035231225192546844,
0.030410248786211014,
0.06159862503409386,
0.02021438628435135,
0.028073808178305626,
-0.10444624722003937,
-0.013133805245161057,
-0.02364058420062065,
0.03429238125681877,
-0.02487124502658844,
-0.05578966438770294,
0.06274038553237915,
-0.022294364869594574,
-0.05305736884474754,
-0.016336996108293533,
-0.0493190661072731,
0.07498271763324738,
0.015836184844374657,
0.0019283595029264688,
0.1178232952952385,
0.06789936870336533,
0.006570340599864721,
0.06293182820081711,
0.04248885065317154,
-0.020886514335870743,
0.07105156034231186,
-0.0009045778424479067,
-0.047157399356365204,
0.0364307276904583,
0.001815362018533051,
0.02767377346754074,
-0.0252988338470459,
-0.04135332256555557,
-0.05134853348135948,
-0.045407187193632126,
-0.06276378035545349,
-0.0015181716298684478,
0.1568371057510376,
-0.026212505996227264,
0.03722849115729332,
0.04790012910962105,
-0.01914668083190918,
0.021756114438176155,
0.037091877311468124,
-0.03344126045703888,
0.029632054269313812,
-0.021618224680423737,
0.006063173525035381,
0.01537418644875288,
-0.010671687312424183,
0.005299015436321497,
0.0021833900827914476,
-0.043131615966558456,
0.009548848494887352,
-0.003955306485295296,
-0.019847797229886055,
0.059606507420539856,
-0.024693872779607773,
-0.019441070035099983,
-0.05628027394413948,
0.009890158660709858,
-0.030576128512620926,
0.015199214220046997,
-0.0030240025371313095,
-0.04468977451324463,
-0.013211242854595184,
0.007640466559678316,
0.043935470283031464,
0.032324325293302536,
0.043638166040182114,
0.007253067567944527,
-0.007350034546107054,
0.010439105331897736,
0.009125855751335621,
-0.06825701892375946,
0.01694370061159134,
0.051787134259939194,
-0.05601947382092476,
0.005142620299011469,
-0.010079300962388515,
-0.0012485315091907978,
0.05204244703054428,
-0.04070288687944412,
-0.0530337393283844,
-0.05396392568945885,
-0.029498817399144173,
-0.022904885932803154,
-0.0659593790769577,
-0.03970138728618622,
0.03326570242643356,
-0.009973730891942978,
0.018130702897906303,
0.04183341935276985,
0.029924089089035988,
0.003110746154561639,
0.06926675885915756,
0.04734909161925316,
0.049943502992391586,
-0.08162030577659607,
0.019810514524579048,
0.06396201252937317,
0.02463432215154171,
0.006631125696003437,
0.01126839965581894,
-0.029211241751909256,
0.0018415197264403105,
-0.06122315675020218,
0.03242511674761772,
0.03187660500407219,
-0.05409112200140953,
0.03625353053212166,
-0.037483617663383484,
0.006550638936460018,
-0.02358452044427395,
-0.061031218618154526,
-0.2675319314002991,
-0.05414780229330063,
0.010242915712296963,
0.012268180027604103,
0.015715889632701874,
-0.04999589920043945,
0.020757056772708893,
-0.02285589650273323,
0.004352835472673178,
-0.05685858055949211,
0.07822877168655396,
0.06769423186779022,
-0.021718882024288177,
0.013224237598478794,
-0.005987164564430714,
0.054506659507751465,
0.015444612130522728,
-0.013774055056273937,
0.003320645075291395,
-0.014937395229935646,
0.014355276711285114,
-0.05258176848292351,
0.054011378437280655,
0.022095073014497757,
0.0862770527601242,
-0.0013284961460158229,
0.09429628401994705,
0.027616729959845543,
-0.03975951671600342,
-0.013961286284029484,
0.05858919769525528,
0.03758400306105614,
0.017309436574578285,
-0.11502283066511154,
-0.0012536760186776519,
-0.07773624360561371,
-0.044443801045417786,
-0.02030334621667862,
-0.05021525174379349,
-0.007572604343295097,
-0.074696384370327,
-0.015063443221151829,
0.002757736947387457,
-0.0041102818213403225,
-0.025482358410954475,
-0.005167752504348755,
0.03419359028339386,
0.03221147879958153,
0.020904621109366417,
-0.038617268204689026,
0.04665178433060646,
-0.008552425540983677,
0.03057084232568741,
-0.04322901740670204,
-0.03878509998321533,
-0.04043564200401306,
-0.026893150061368942,
0.02197522297501564,
-0.07259123772382736,
0.015810195356607437,
-0.016554348170757294,
-0.02659768797457218,
-0.04096314311027527,
0.05135428160429001,
-0.021724123507738113,
-0.025578657165169716,
-0.06609392911195755,
-0.04148895666003227,
-0.0006670398870483041,
-0.005408128723502159,
0.01817229762673378,
0.11393685638904572,
-0.023280076682567596,
-0.014357578940689564,
-0.0393073596060276,
-0.0157461054623127,
-0.008953051641583443,
-0.02671852521598339,
-0.08706209808588028,
0.013064103201031685,
-0.0008890609024092555,
-0.04135221242904663,
0.0832386389374733,
0.006463001947849989,
-0.030680716037750244,
0.09782875329256058,
0.009112914092838764,
0.002933353651314974,
0.05444936826825142,
0.04664396494626999,
0.03451475128531456,
0.013224721886217594,
-0.03905113413929939,
-0.06018327549099922,
0.04021915793418884,
0.00913796853274107,
-0.27509284019470215,
-0.009522618725895882,
-0.0018310976447537541,
-0.021870996803045273,
-0.02999274805188179,
0.021683985367417336,
0.03439812734723091,
-0.009111088700592518,
-0.036894943565130234,
0.08133487403392792,
0.05781879276037216,
0.039286933839321136,
0.08052868396043777,
-0.0022728536278009415,
-0.0529315248131752,
0.03829040378332138,
0.052588868886232376,
0.045199088752269745,
0.03960784152150154,
-0.039221614599227905,
0.002648530527949333,
-0.03149671480059624,
0.13832277059555054,
0.006604313384741545,
0.036661941558122635,
0.0404878593981266,
0.018141701817512512,
0.014317438006401062,
-0.03570426255464554,
-0.004781677387654781,
-0.019380517303943634,
0.03886989876627922,
0.04919872805476189,
-0.009258512407541275,
-0.030339036136865616,
0.08111488074064255,
-0.01473324280232191,
0.00730568403378129,
-0.013257504440844059,
-0.013555948622524738,
0.07883778214454651,
0.03306714817881584,
-0.07143294811248779,
-0.012427628971636295,
0.05537335202097893,
-0.10463967174291611,
-0.0421924814581871,
-0.04734861105680466,
0.038174595683813095,
-0.012827926315367222,
-0.06397303193807602,
-0.055996883660554886,
0.027287008240818977,
0.06459514796733856,
-0.015569704584777355,
-0.017892302945256233,
-0.041538018733263016,
0.024046357721090317,
0.0031384325120598078,
-0.02967485412955284,
0.04279590770602226,
0.024472886696457863,
-0.06071832403540611,
0.09726186096668243,
0.03556641563773155
] |
PMID:8395 | A new model for antisperm autoimmunity in guine pigs. | Adult outbread male guinea pigs were autoimmunized without adjuvant. Homogenates were prepared with one of their own testes previously submitted "in vivo" and "in vitro" to thermal injury. Animals received a single or daily repeated intradermal injection without added adjuvant, in one or different skin sites. Guinea pigs daily sensitized in the same site during 30 days showed the presence of: a) dermal granuloma at the site of injection; b) several foci of typical allergic orchitis; c) delayed hypersensitivity detected by inhibition of macrophage migration; d) moderate titres of spermagglutinins and negligible levels of hemagglutinating antibodies. Guinea pigs receiving a single dose in one site only developed delayed hypersensitivity. Animals daily sensitized with the same dose of altered antigen in different sites, or with normal testis antigen either in one or different sites, showed negative results. The correlation among testicular lesion, dermal granuloma and cellular immunity is discussed. It is concluded that testis autosensitization is obtained in the absence of added adjuvant provided that a thermally injured gonad used as antigen is repeatedly injected in the same site. | A new model for antisperm autoimmunity in guine pigs. Adult outbread male guinea pigs were autoimmunized without adjuvant. Homogenates were prepared with one of their own testes previously submitted "in vivo" and "in vitro" to thermal injury. Animals received a single or daily repeated intradermal injection without added adjuvant, in one or different skin sites. Guinea pigs daily sensitized in the same site during 30 days showed the presence of: a) dermal granuloma at the site of injection; b) several foci of typical allergic orchitis; c) delayed hypersensitivity detected by inhibition of macrophage migration; d) moderate titres of spermagglutinins and negligible levels of hemagglutinating antibodies. Guinea pigs receiving a single dose in one site only developed delayed hypersensitivity. Animals daily sensitized with the same dose of altered antigen in different sites, or with normal testis antigen either in one or different sites, showed negative results. The correlation among testicular lesion, dermal granuloma and cellular immunity is discussed. It is concluded that testis autosensitization is obtained in the absence of added adjuvant provided that a thermally injured gonad used as antigen is repeatedly injected in the same site. | [
-0.016395170241594315,
0.055734653025865555,
-0.00942721776664257,
0.03822251781821251,
0.029469048604369164,
0.022139647975564003,
0.0642777681350708,
0.057350944727659225,
-0.057733431458473206,
-0.04856901243329048,
0.027919625863432884,
-0.09840874373912811,
0.04525095224380493,
-0.002465860452502966,
-0.006613206118345261,
0.026744695380330086,
-0.021685568615794182,
-0.008356979116797447,
-0.03181862086057663,
-0.007470603100955486,
-0.025479497388005257,
0.006764656864106655,
0.015675129368901253,
-0.011048782616853714,
0.017838841304183006,
0.0010941388318315148,
0.020137205719947815,
0.027268869802355766,
-0.008528552018105984,
-0.1713908463716507,
-0.0026078205555677414,
-0.041485533118247986,
-0.03525319695472717,
0.009225930087268353,
-0.024442603811621666,
-0.05615422874689102,
-0.01959996670484543,
-0.025856805965304375,
0.013890647329390049,
0.02967311069369316,
0.10928080230951309,
0.046325698494911194,
-0.012636966072022915,
0.053482428193092346,
0.0657067522406578,
0.04533223435282707,
-0.03329690173268318,
-0.0352390855550766,
0.04033278301358223,
0.00939050130546093,
0.03966329246759415,
-0.022785626351833344,
0.015570079907774925,
0.0697532668709755,
-0.027044521644711494,
-0.03153910115361214,
-0.014770740643143654,
0.028248200193047523,
-0.037644535303115845,
0.023847833275794983,
0.020420202985405922,
0.03528181090950966,
-0.09138554334640503,
0.096210777759552,
0.04924146458506584,
-0.040420085191726685,
-0.008784990757703781,
-0.04554819315671921,
0.039153631776571274,
0.02698848396539688,
-0.016673432663083076,
0.0465523824095726,
-0.0045385719276964664,
0.006073446478694677,
0.006978266406804323,
0.0019325206521898508,
0.008229358121752739,
0.015982206910848618,
0.05428468436002731,
0.026052292436361313,
-0.033408693969249725,
0.025239134207367897,
0.012861092574894428,
-0.02259441837668419,
-0.07069239765405655,
-0.09454186260700226,
0.030340274795889854,
-0.08491946756839752,
0.03858092799782753,
0.029476162046194077,
0.019197208806872368,
-0.03001944161951542,
0.028194326907396317,
-0.01980607956647873,
-0.04124395549297333,
0.03519831970334053,
0.01785098761320114,
-0.02012566290795803,
-0.001179700717329979,
0.42386138439178467,
-0.008012312464416027,
-0.042252641171216965,
-0.06500836461782455,
-0.024984775111079216,
0.006717458367347717,
0.009527809917926788,
-0.053636446595191956,
-0.011645544320344925,
-0.05253986269235611,
0.07451516389846802,
0.008771897293627262,
0.021555868908762932,
0.012315517291426659,
-0.014024801552295685,
-0.03140940144658089,
-0.016452569514513016,
0.06540990620851517,
0.002122581237927079,
0.008444353006780148,
0.03530021756887436,
0.008601068519055843,
-0.05615069717168808,
0.0009787394665181637,
-0.06277698278427124,
0.03984447941184044,
-0.0006388940964825451,
0.039303988218307495,
0.07294126600027084,
-0.006704995408654213,
-0.009582625702023506,
0.041454017162323,
0.010136507451534271,
0.029018552973866463,
0.00408915663138032,
0.043751005083322525,
-0.0035011803265661,
0.020808765664696693,
-0.0023185014724731445,
-0.03469886630773544,
-0.00920408871024847,
0.02798166312277317,
-0.07378166168928146,
-0.028311211615800858,
-0.06002715975046158,
0.03493396192789078,
0.04320068657398224,
0.008969630114734173,
-0.008316891267895699,
-0.03740091249346733,
0.054610490798950195,
0.022644536569714546,
0.054195959120988846,
-0.06169017776846886,
0.06486418098211288,
-0.02839014120399952,
0.039888765662908554,
-0.046859994530677795,
0.030939949676394463,
0.010961643420159817,
-0.029080979526042938,
-0.0558319054543972,
-0.04165695607662201,
-0.051032062619924545,
0.06983642280101776,
0.042849101126194,
-0.0598255954682827,
-0.03677147626876831,
0.039057645946741104,
0.0084722600877285,
-0.0004156485083512962,
0.009675639681518078,
0.0027055409736931324,
-0.0418224073946476,
-0.019850049167871475,
-0.05449141934514046,
0.010906915180385113,
-0.022864213213324547,
-0.0005637239664793015,
0.01870289072394371,
0.10070998966693878,
-0.02115374058485031,
-0.05427034944295883,
-0.0071480306796729565,
0.016586212441325188,
-0.013145255856215954,
0.005173335783183575,
-0.07678154855966568,
0.02408272586762905,
0.02577739953994751,
-0.01740742102265358,
0.028411943465471268,
-0.04319019988179207,
-0.07497158646583557,
-0.004942518658936024,
-0.012633131816983223,
-0.006369335111230612,
-0.006463116966187954,
-0.023702142760157585,
0.08715346455574036,
-0.012861869297921658,
0.07729325443506241,
-0.047951534390449524,
0.04471924528479576,
-0.006521372590214014,
0.021848514676094055,
0.02283610962331295,
0.03279581665992737,
-0.035890620201826096,
0.09242509305477142,
-0.0385211706161499,
-0.08362764120101929,
0.024043390527367592,
-0.017083941027522087,
-0.026981256902217865,
-0.019014474004507065,
0.05833233520388603,
-0.02949666604399681,
-0.07641136646270752,
0.07523899525403976,
0.01725178211927414,
-0.02167307399213314,
0.07235829532146454,
-0.006585664581507444,
-0.3029036223888397,
-0.07306335121393204,
0.014090115204453468,
-0.038998108357191086,
0.054536689072847366,
-0.06140957027673721,
-0.0024764579720795155,
-0.05059419572353363,
0.05659949779510498,
-0.016630813479423523,
0.08079050481319427,
0.02696938067674637,
-0.03229864686727524,
0.02524377591907978,
0.003008897416293621,
0.050101932138204575,
0.01512904092669487,
-0.016007259488105774,
-0.01284126378595829,
0.06029342859983444,
-0.018087659031152725,
-0.001534807845018804,
0.045328445732593536,
0.032452985644340515,
0.032064396888017654,
-0.01178706530481577,
0.12307251989841461,
0.04354194924235344,
-0.025766558945178986,
-0.04997146502137184,
0.033730052411556244,
-0.00047386548249050975,
-0.01167234405875206,
-0.029779719188809395,
-0.03381533920764923,
-0.0005528225447051227,
-0.04770248383283615,
0.02007075771689415,
0.000013037527423875872,
0.022088034078478813,
-0.015269399620592594,
-0.004510400351136923,
-0.056317489594221115,
-0.07057596743106842,
-0.03921994939446449,
-0.004550114739686251,
0.03616640344262123,
0.003938683308660984,
-0.03763478249311447,
0.006112637463957071,
0.04167323559522629,
-0.08045255392789841,
-0.007341931574046612,
-0.006364917382597923,
-0.02320856787264347,
-0.05081156641244888,
-0.0507190078496933,
-0.004503373056650162,
-0.020673394203186035,
0.028798824176192284,
0.07054576277732849,
0.00039145906339399517,
0.03517698869109154,
-0.06255687773227692,
-0.06789349019527435,
0.012158598750829697,
0.03615424782037735,
-0.0598447248339653,
0.011945507489144802,
0.005676044151186943,
0.02934240736067295,
0.14176195859909058,
0.010646299459040165,
0.008718091994524002,
0.017931997776031494,
-0.024861976504325867,
0.010844837874174118,
0.011397984810173512,
-0.08954674750566483,
0.0157155804336071,
0.046271421015262604,
-0.023647526279091835,
0.009207602590322495,
0.024132631719112396,
0.020965086296200752,
0.018379999324679375,
-0.016752533614635468,
0.0034749445039778948,
-0.00012278569920454174,
0.03905494511127472,
-0.0330347940325737,
-0.001210952759720385,
-0.03778069466352463,
-0.012810708954930305,
0.005172453820705414,
-0.02367338538169861,
-0.27987974882125854,
-0.009015410207211971,
0.021663857623934746,
0.05218039080500603,
-0.06177794188261032,
-0.024604981765151024,
0.02363460697233677,
-0.0424480177462101,
-0.05470966175198555,
0.003350929357111454,
0.05615266039967537,
0.010801807045936584,
0.022351127117872238,
0.021960409358143806,
-0.020247096195816994,
0.023955311626195908,
-0.02290511131286621,
0.017122626304626465,
-0.056324705481529236,
-0.03436094522476196,
0.01593063771724701,
-0.08706717938184738,
0.14427539706230164,
0.029664959758520126,
0.00861863512545824,
0.056315213441848755,
0.00008984645683085546,
0.024843361228704453,
-0.0045370375737547874,
0.009870525449514389,
0.011357955634593964,
-0.009383057244122028,
0.012135456316173077,
0.02996576949954033,
0.03066808171570301,
-0.00993811059743166,
-0.01812429539859295,
0.03740234673023224,
0.022435840219259262,
0.012551110237836838,
-0.07872792333364487,
0.022863183170557022,
-0.0036669576074928045,
-0.048733796924352646,
0.049870897084474564,
-0.033731769770383835,
-0.013499262742698193,
-0.030139710754156113,
-0.010301763191819191,
0.009011572226881981,
0.023687154054641724,
-0.07508963346481323,
0.04939943552017212,
0.02427813969552517,
0.03932521864771843,
-0.0765313059091568,
0.07780754566192627,
0.019150013104081154,
0.0009918289724737406,
0.030460193753242493,
0.022665787488222122,
-0.005943777970969677,
-0.07365074008703232,
0.06349232792854309,
0.006438480690121651
] |
PMID:8397 | Quantitative study of spermatogenesis in vasectomized mice. | Adult mice were vasectomized under semi-sterile conditions and examined five weeks later. The weight of the epididymis was increased but none of the animals had developed spermatic granulomas. The concentration of testosterone in the plasma, the body weight and the weight of the testes were not affected by vasectomy. Cross sections of the testes were prepared for histological examination and the nuclei of the various types of germinal cells were enumerated at stage VII of spermatogenesis. Vasectomy caused a small, but statistically significant, decrease in the number of preleptotene spermatocytes, pachytene spermatocytes and step 7 spermatids. | Quantitative study of spermatogenesis in vasectomized mice. Adult mice were vasectomized under semi-sterile conditions and examined five weeks later. The weight of the epididymis was increased but none of the animals had developed spermatic granulomas. The concentration of testosterone in the plasma, the body weight and the weight of the testes were not affected by vasectomy. Cross sections of the testes were prepared for histological examination and the nuclei of the various types of germinal cells were enumerated at stage VII of spermatogenesis. Vasectomy caused a small, but statistically significant, decrease in the number of preleptotene spermatocytes, pachytene spermatocytes and step 7 spermatids. | [
0.017314013093709946,
0.03782946243882179,
0.06155586615204811,
-0.04973223805427551,
-0.005985373165458441,
0.047852788120508194,
0.030670667067170143,
0.03937521576881409,
-0.05116409435868263,
0.019717732444405556,
0.023388870060443878,
-0.03207669407129288,
0.026747921481728554,
0.014186205342411995,
-0.014080913737416267,
-0.03372712433338165,
-0.021650144830346107,
0.040773604065179825,
-0.09035702794790268,
0.04826733469963074,
0.036703966557979584,
-0.005877647083252668,
0.028923552483320236,
-0.02354569360613823,
0.017519203945994377,
-0.02329927496612072,
-0.03220885246992111,
0.004039046820253134,
-0.041786812245845795,
-0.14529597759246826,
0.038452599197626114,
-0.0478789322078228,
-0.026036888360977173,
0.006044147536158562,
0.011235680431127548,
0.015385165810585022,
-0.019443582743406296,
0.003524565137922764,
0.01324870903044939,
0.00213812617585063,
0.05636126920580864,
0.025630725547671318,
-0.029340429231524467,
0.033270057290792465,
0.014271031133830547,
0.029455257579684258,
-0.02850079908967018,
-0.03412933275103569,
0.09427160769701004,
0.03530267998576164,
0.030872449278831482,
-0.03189637511968613,
0.03813479468226433,
0.04522882029414177,
-0.06533357501029968,
-0.02473278157413006,
0.03781463950872421,
0.04400523006916046,
-0.002206629142165184,
0.03493725508451462,
-0.02868533879518509,
0.017131835222244263,
-0.11990402638912201,
0.06677962094545364,
0.06776363402605057,
-0.03795742988586426,
-0.035512130707502365,
-0.08261002600193024,
0.04869648814201355,
-0.011982067488133907,
-0.001811008551158011,
0.07364127039909363,
0.041554614901542664,
-0.004868797492235899,
-0.03184761106967926,
-0.05165652185678482,
-0.05737144500017166,
-0.03117852658033371,
0.013115630485117435,
-0.001983625115826726,
-0.05610419064760208,
-0.027185801416635513,
0.008598515763878822,
0.01723969355225563,
-0.10614494979381561,
-0.04110538959503174,
-0.008516859263181686,
-0.056488458067178726,
0.03407522663474083,
0.04573679715394974,
0.010118653997778893,
-0.026595832780003548,
-0.054376810789108276,
-0.01416031550616026,
-0.006413359194993973,
0.027373630553483963,
0.005026150960475206,
-0.04024732857942581,
0.018799519166350365,
0.39879778027534485,
0.004314007703214884,
0.0031008559744805098,
-0.03462760150432587,
-0.02995259501039982,
-0.01677101105451584,
0.035569608211517334,
-0.04940710589289665,
0.004610627889633179,
-0.0016065953532233834,
0.08521778136491776,
0.0711125060915947,
0.0006707349675707519,
0.0572085976600647,
-0.02655346877872944,
-0.05005510523915291,
-0.015961138531565666,
0.07559048384428024,
0.0581921711564064,
-0.00752467755228281,
0.037563715130090714,
-0.03148698806762695,
0.009051421657204628,
0.024814972653985023,
-0.053747374564409256,
0.06487347930669785,
0.0016181981191039085,
-0.01610691472887993,
0.05986613407731056,
-0.02833058498799801,
-0.03548601269721985,
0.03191988542675972,
0.049310121685266495,
-0.05361367389559746,
0.03074313886463642,
0.012257634662091732,
0.010041086003184319,
0.04408910125494003,
-0.06174823269248009,
0.017027370631694794,
-0.012950645759701729,
-0.01645958051085472,
-0.028077008202672005,
-0.06417714059352875,
-0.0787704586982727,
0.027131039649248123,
0.09379204362630844,
0.04790192469954491,
-0.01867576129734516,
-0.0160346832126379,
0.07388785481452942,
0.03832944482564926,
0.04008865728974342,
-0.037827201187610626,
0.03431521728634834,
-0.03520960733294487,
0.0217779241502285,
-0.02322225831449032,
-0.0035268403589725494,
0.019773583859205246,
-0.008243178948760033,
-0.024803508073091507,
-0.0018058768473565578,
-0.013186055235564709,
0.01463148184120655,
0.06953906267881393,
-0.01505958754569292,
-0.04764057695865631,
0.07497796416282654,
-0.021382229402661324,
-0.02107613906264305,
-0.034411393105983734,
-0.0025274562649428844,
-0.04767464101314545,
0.004028670489788055,
-0.04653880000114441,
-0.026986833661794662,
0.005618303082883358,
0.03198997676372528,
0.012541421689093113,
0.05281107500195503,
0.039057571440935135,
-0.05469260364770889,
-0.023831836879253387,
0.05007629469037056,
-0.038713231682777405,
0.0020370343700051308,
-0.06484661996364594,
0.06556123495101929,
0.04519205540418625,
0.023699652403593063,
-0.0027405486907809973,
-0.05772489681839943,
-0.07039293646812439,
-0.0031437373254448175,
0.01472524181008339,
-0.016333362087607384,
-0.02571871504187584,
-0.027912607416510582,
-0.029017629101872444,
-0.003201568266376853,
0.04868459329009056,
-0.0405973382294178,
0.08499601483345032,
0.0582105815410614,
0.015950612723827362,
0.0618559904396534,
0.052937254309654236,
0.014273992739617825,
0.09563971310853958,
0.04398205503821373,
-0.07276639342308044,
-0.003875849535688758,
-0.03014427423477173,
0.02711530029773712,
-0.043185893446207047,
0.07811934500932693,
0.02342434786260128,
-0.051783718168735504,
0.08623076230287552,
-0.04481302946805954,
0.035423923283815384,
0.029736561700701714,
0.0022666314616799355,
-0.32458484172821045,
-0.06354282051324844,
-0.010656251572072506,
-0.021769436076283455,
0.034029196947813034,
-0.07924950122833252,
0.03504861518740654,
-0.032511141151189804,
0.03744709491729736,
-0.0748644769191742,
0.04907197877764702,
0.03040442243218422,
-0.02111608348786831,
0.02619108371436596,
-0.049255337566137314,
-0.02160588651895523,
0.008512206375598907,
-0.07268349826335907,
-0.02532290481030941,
0.02367226406931877,
-0.047377631068229675,
-0.03158650919795036,
-0.030254516750574112,
0.03799145668745041,
0.08382707089185715,
-0.049406491219997406,
0.10623730719089508,
-0.003963088616728783,
-0.04452487453818321,
-0.02496071718633175,
0.04941251501441002,
0.019391167908906937,
0.017404643818736076,
-0.0654364749789238,
0.016219347715377808,
-0.037416599690914154,
-0.09301084280014038,
-0.017987292259931564,
-0.042448144406080246,
0.03843770548701286,
-0.06701230257749557,
-0.033263131976127625,
-0.033611737191677094,
-0.0660855621099472,
-0.03587530925869942,
-0.001716258586384356,
0.02795041725039482,
0.01748669520020485,
0.003087651217356324,
0.03736318647861481,
0.03993265703320503,
-0.011276365257799625,
0.0016807528445497155,
-0.049121059477329254,
0.004038945771753788,
-0.021659718826413155,
-0.059169232845306396,
0.014849926345050335,
-0.049817003309726715,
0.05130892992019653,
0.06901831179857254,
0.01534227468073368,
0.020424384623765945,
-0.05172467604279518,
-0.024970708414912224,
-0.06451115757226944,
-0.03683886304497719,
-0.044318463653326035,
0.015821410343050957,
-0.03814389184117317,
0.023551132529973984,
0.10598526895046234,
-0.005425706505775452,
0.04295477271080017,
0.04781961441040039,
0.010457741096615791,
-0.005053295288234949,
-0.0018031139625236392,
-0.02419992908835411,
-0.038728442043066025,
0.015689073130488396,
-0.028714172542095184,
0.029521537944674492,
-0.04396946728229523,
0.014140035025775433,
-0.005210452247411013,
-0.062461480498313904,
-0.006979241967201233,
0.019405590370297432,
-0.023928143084049225,
-0.013637477532029152,
0.002980192191898823,
-0.014185067266225815,
0.01878206804394722,
-0.007648015394806862,
0.02636099047958851,
-0.2731057107448578,
-0.05306597426533699,
0.05667077377438545,
0.03819558024406433,
0.022251706570386887,
0.03174416348338127,
0.020479226484894753,
-0.009920001029968262,
0.014718621969223022,
0.046839095652103424,
0.059462770819664,
-0.021407436579465866,
0.03959814831614494,
-0.007278389297425747,
-0.02505127154290676,
0.016679245978593826,
0.025828078389167786,
0.017296262085437775,
0.011759973131120205,
-0.050004858523607254,
0.04689399525523186,
-0.08855214715003967,
0.11088889837265015,
-0.018512675538659096,
-0.027505483478307724,
0.04267952963709831,
0.024057231843471527,
0.01538180559873581,
-0.024304477497935295,
0.01279406901448965,
-0.0031357957050204277,
0.005730245728045702,
-0.03371215984225273,
-0.018304165452718735,
0.058158546686172485,
0.034177832305431366,
-0.06157715991139412,
0.014297501184046268,
0.008905865252017975,
0.012866626493632793,
-0.015264129266142845,
0.005825546104460955,
-0.03131556883454323,
-0.0065284352749586105,
0.0725158080458641,
-0.011644396930932999,
-0.007367826532572508,
0.024543533101677895,
0.026529429480433464,
0.021309616044163704,
-0.008152785710990429,
0.006373087875545025,
0.020189857110381126,
0.04144800826907158,
-0.020262278616428375,
-0.06395278126001358,
0.06327876448631287,
-0.03479986637830734,
0.0908590629696846,
0.06122630834579468,
0.01516859419643879,
-0.002873203484341502,
-0.057638637721538544,
0.03906900808215141,
0.03696075454354286
] |
PMID:8398 | Fertility patterns in female mice following treatment with arginine vastocin or melatonin. | Daily injections of either 2 mug arginine vasotocin (AVT) or 100 mug melatonin significantly prolonged the estrous cycles of mature female mice. If administered daily throughout pregnancy, none of the AVT-treated mice delivered viable pups. | Fertility patterns in female mice following treatment with arginine vastocin or melatonin. Daily injections of either 2 mug arginine vasotocin (AVT) or 100 mug melatonin significantly prolonged the estrous cycles of mature female mice. If administered daily throughout pregnancy, none of the AVT-treated mice delivered viable pups. | [
-0.019276803359389305,
0.0007876135059632361,
-0.03813686966896057,
-0.006442244164645672,
0.036018602550029755,
0.06390757858753204,
-0.007443729322403669,
0.030623121187090874,
-0.01370109524577856,
-0.04195236787199974,
-0.015451492741703987,
-0.03835822269320488,
-0.02665919065475464,
0.011297264136373997,
0.002045193687081337,
-0.014108589850366116,
-0.037226513028144836,
-0.040387146174907684,
-0.046597253531217575,
0.011873929761350155,
0.01805315911769867,
-0.0204048790037632,
0.011228997260332108,
-0.02010580152273178,
0.06879935413599014,
-0.007794579025357962,
-0.03135465830564499,
-0.0543944276869297,
-0.05577242001891136,
-0.15469807386398315,
-0.022188615053892136,
-0.04492617025971413,
-0.02155236341059208,
0.034252364188432693,
-0.053355246782302856,
-0.0065842922776937485,
-0.05784980580210686,
0.026364155113697052,
-0.036677081137895584,
0.047203656286001205,
0.10060203820466995,
-0.003946882672607899,
-0.03832360357046127,
-0.050617728382349014,
0.029543951153755188,
0.009575688280165195,
-0.00832619983702898,
-0.02850571647286415,
0.06030665710568428,
0.003649845952168107,
0.07502765208482742,
-0.03418029472231865,
0.028026316314935684,
0.03364947438240051,
-0.008781449869275093,
0.018791159614920616,
-0.04065977409482002,
-0.0004943591193296015,
0.009545322507619858,
0.07902450859546661,
0.0009971550898626447,
0.06279583275318146,
-0.14416776597499847,
0.08099545538425446,
0.02018435113132,
-0.006390525493770838,
0.0015613544965162873,
0.006810296792536974,
0.0014257525326684117,
0.037324100732803345,
-0.005247630178928375,
0.01849484257400036,
-0.019415482878684998,
-0.014089609496295452,
0.043713271617889404,
-0.033417630940675735,
0.05851227790117264,
-0.06349880993366241,
0.040903862565755844,
-0.021511079743504524,
-0.033484719693660736,
0.011602601036429405,
0.035815879702568054,
-0.06307519227266312,
0.04226800426840782,
-0.08733253926038742,
0.013770629651844501,
-0.03550271317362785,
0.05470464751124382,
0.014141278341412544,
-0.04741630330681801,
0.00025475345319136977,
-0.027350546792149544,
-0.06683845072984695,
-0.012766587547957897,
0.01849329099059105,
0.016791965812444687,
-0.03929470106959343,
-0.03181928023695946,
0.35350722074508667,
-0.01958891935646534,
0.0013238643296062946,
-0.11992105096578598,
-0.02853715606033802,
0.010122007690370083,
0.008700571022927761,
-0.0026406303513795137,
-0.0173420999199152,
0.00289939739741385,
0.07293272018432617,
-0.010492362082004547,
0.0644308477640152,
0.06040420010685921,
-0.010865852236747742,
0.0041990880854427814,
-0.012307317927479744,
0.07510817050933838,
-0.0027591832913458347,
-0.021415211260318756,
0.04749476909637451,
-0.015013239346444607,
-0.05219995975494385,
0.04448312520980835,
-0.04656146839261055,
0.06834759563207626,
-0.018395189195871353,
-0.0019363367464393377,
-0.009885849431157112,
0.03193097561597824,
0.026694366708397865,
0.0569266751408577,
0.10092224180698395,
-0.0012209651758894324,
0.02266569435596466,
0.05823831632733345,
-0.011082177981734276,
-0.0026414920575916767,
-0.03320514038205147,
-0.019949544221162796,
0.009381316602230072,
-0.04620300605893135,
-0.036774054169654846,
0.030951282009482384,
-0.08510347455739975,
0.03940454125404358,
0.1114397868514061,
0.05867346376180649,
0.036977335810661316,
0.025561504065990448,
0.059949859976768494,
0.01683042012155056,
0.04191971197724342,
-0.03907354176044464,
0.0028822626918554306,
0.010364812798798084,
0.05565332993865013,
-0.08006469160318375,
0.017964880913496017,
-0.03891032934188843,
-0.035605691373348236,
-0.008937859907746315,
-0.017112281173467636,
-0.07621864229440689,
-0.011775569058954716,
0.007650452200323343,
-0.019557781517505646,
-0.04179253429174423,
0.010608027689158916,
-0.05158228427171707,
-0.045213136821985245,
0.009509051218628883,
0.017216213047504425,
0.019147442653775215,
-0.013586562126874924,
-0.020286303013563156,
0.00556991808116436,
-0.005800381302833557,
0.014979190193116665,
-0.00418942840769887,
0.047784432768821716,
-0.008996106684207916,
-0.028761597350239754,
0.011430280283093452,
0.06932736188173294,
0.052279651165008545,
-0.05701683089137077,
-0.023160625249147415,
-0.006238346919417381,
-0.029223358258605003,
0.018046909943223,
0.013815365731716156,
-0.008096769452095032,
-0.01687726005911827,
-0.020705820992588997,
0.001532904920168221,
-0.05381172522902489,
-0.04500885307788849,
0.012715558521449566,
0.004881765227764845,
-0.024075720459222794,
0.07494616508483887,
-0.03633659705519676,
0.03118503838777542,
0.060270726680755615,
0.02693329192698002,
0.0959760993719101,
-0.02721264772117138,
-0.08521644026041031,
0.0673828050494194,
-0.07013674080371857,
-0.011892472393810749,
0.049659084528684616,
0.0556657500565052,
0.005346826743334532,
-0.12081129103899002,
0.1385708600282669,
0.010701914317905903,
0.04090912267565727,
0.08146468549966812,
0.030857916921377182,
0.07723226398229599,
-0.024023009464144707,
0.029772156849503517,
-0.3056643307209015,
-0.017744489014148712,
0.0006933807162567973,
-0.07825926691293716,
0.06767108291387558,
-0.08256497979164124,
-0.020018354058265686,
-0.0724581927061081,
0.050579581409692764,
-0.030821938067674637,
0.040868695825338364,
0.04814302548766136,
-0.02085098810493946,
-0.04953397437930107,
0.04680204018950462,
0.020394612103700638,
0.035006556659936905,
-0.07294616848230362,
-0.002016864251345396,
-0.04639260470867157,
-0.05779764801263809,
-0.03996807709336281,
-0.016160329803824425,
0.03789953142404556,
0.025872187688946724,
0.0218121949583292,
0.14079636335372925,
0.023279065266251564,
-0.015128175728023052,
-0.010159260593354702,
0.03380216285586357,
-0.022986484691500664,
-0.03282982483506203,
-0.05791087448596954,
0.01626933179795742,
0.024393748492002487,
-0.007836480624973774,
0.029007889330387115,
-0.0035225029569119215,
0.024897560477256775,
-0.03211399167776108,
0.024785775691270828,
-0.05207983776926994,
-0.02082599326968193,
-0.03911448270082474,
0.013616267591714859,
0.03930401802062988,
0.02281942218542099,
0.013011851347982883,
-0.023356445133686066,
0.04652995988726616,
0.07184519618749619,
-0.012137126177549362,
-0.016286008059978485,
0.010420477949082851,
-0.04524368420243263,
-0.07974257320165634,
0.03497759625315666,
-0.022141866385936737,
-0.022823739796876907,
0.05507140979170799,
0.011500566266477108,
0.000049451395170763135,
-0.01504526287317276,
-0.06786228716373444,
0.0034876337740570307,
-0.02954350970685482,
-0.011042695492506027,
0.06867536157369614,
-0.015309679321944714,
0.008308151736855507,
0.10453736782073975,
-0.05198691040277481,
0.036699339747428894,
0.043443288654088974,
0.0024683407973498106,
-0.0036306455731391907,
-0.006164805963635445,
-0.0724320188164711,
0.011416211724281311,
-0.04110773280262947,
-0.04758381471037865,
0.027131211012601852,
0.034383948892354965,
-0.04054708778858185,
0.03587851673364639,
-0.028725534677505493,
0.0002087182510877028,
0.08770546317100525,
0.009390384890139103,
0.010971708223223686,
-0.002649048576131463,
-0.0031516633462160826,
-0.017536824569106102,
-0.007612540386617184,
0.052791815251111984,
-0.2533494830131531,
-0.040092796087265015,
0.041631292551755905,
0.011198651045560837,
-0.030717486515641212,
-0.010423998348414898,
-0.011242601089179516,
-0.03145189955830574,
-0.04057073965668678,
0.023794595152139664,
0.04161602258682251,
-0.01869744434952736,
0.05945014953613281,
0.06369616836309433,
-0.02016277052462101,
0.01843893714249134,
0.03741365298628807,
0.03156452625989914,
-0.030967315658926964,
-0.03248243033885956,
0.03836841881275177,
-0.052910253405570984,
0.12274610996246338,
-0.036302875727415085,
-0.04337532818317413,
0.038557957857847214,
-0.0026279063895344734,
0.06213447451591492,
0.01747007668018341,
0.001377771724946797,
-0.034432291984558105,
0.04292946681380272,
0.04060973599553108,
-0.043878067284822464,
0.09845486283302307,
0.0332300141453743,
-0.04494444280862808,
0.013750297948718071,
0.03347315639257431,
0.031087907031178474,
-0.008325472474098206,
0.000317376950988546,
-0.054780878126621246,
-0.01560878288000822,
0.06971238553524017,
-0.07874201238155365,
-0.026355812326073647,
-0.05089723318815231,
-0.018654916435480118,
-0.03934803977608681,
-0.028520504012703896,
-0.0040251342579722404,
0.03495419770479202,
0.029501892626285553,
-0.01184491254389286,
-0.03796512633562088,
0.022132299840450287,
0.013864976353943348,
-0.01672719046473503,
0.04141885042190552,
0.003962930291891098,
-0.024372680112719536,
-0.05421249568462372,
0.07447364926338196,
0.04913217946887016
] |
PMID:8399 | The pH dependence of binding of alpha,alpha'-dibromo-p-xylenesulfonic acid to lysozyme. | The chemical modification of lysozyme (I) has been accomplished with alpha, alpha'-dibromo-p-xylenesulfonic acid (DBX) at five different pH values. I was alkylated by DBX at room temperature (28 degrees C) with decrease in enzyme activity. The rate of inactivation depended upon the pH at which alkylation was carried out. The highest rate was seen at alkaline pH values; the lowest at more acidic pH values. Amino acid analyses showed that-two lysines and two tryptophan residues had been modified at pH 9; two lysines, one tryptophan and one methionine had reacted at pH 8. A histidine residue was bound at pH 6.5 together with a tryptophan residue. At the lower pH values (2.7, 4.5, 6.5), alkylation occurred with a single tryptophan residue each. Fluorescence and CD data both ruled out the participation of tryptophans 62 or 108. Labeling experiments showed that two residues of DBX-35S were bound per molecule of I at both pH9 and pH8; one residue of DBX was bound per molecule of I at the other pH values. Sedimentation coefficients were characteristic of native lysozyme. The stoichiometry of binding and residue modification indicated that intra-molecular cross links were established. The pH dependence of the cross-linking provides means to measure several allowed intra-molecular distances. The results presented here are consistent with the existence of side chain motion in lysozyme. | The pH dependence of binding of alpha,alpha'-dibromo-p-xylenesulfonic acid to lysozyme. The chemical modification of lysozyme (I) has been accomplished with alpha, alpha'-dibromo-p-xylenesulfonic acid (DBX) at five different pH values. I was alkylated by DBX at room temperature (28 degrees C) with decrease in enzyme activity. The rate of inactivation depended upon the pH at which alkylation was carried out. The highest rate was seen at alkaline pH values; the lowest at more acidic pH values. Amino acid analyses showed that-two lysines and two tryptophan residues had been modified at pH 9; two lysines, one tryptophan and one methionine had reacted at pH 8. A histidine residue was bound at pH 6.5 together with a tryptophan residue. At the lower pH values (2.7, 4.5, 6.5), alkylation occurred with a single tryptophan residue each. Fluorescence and CD data both ruled out the participation of tryptophans 62 or 108. Labeling experiments showed that two residues of DBX-35S were bound per molecule of I at both pH9 and pH8; one residue of DBX was bound per molecule of I at the other pH values. Sedimentation coefficients were characteristic of native lysozyme. The stoichiometry of binding and residue modification indicated that intra-molecular cross links were established. The pH dependence of the cross-linking provides means to measure several allowed intra-molecular distances. The results presented here are consistent with the existence of side chain motion in lysozyme. | [
-0.05877018719911575,
-0.04441600665450096,
0.015648912638425827,
0.0014993761433288455,
-0.01543707400560379,
-0.018132729455828667,
0.026307882741093636,
0.01700972579419613,
-0.023444419726729393,
-0.012798548676073551,
0.0077420566231012344,
-0.03986465930938721,
0.0032080032397061586,
0.04049279913306236,
-0.04956694692373276,
-0.039729222655296326,
-0.040433306246995926,
0.004213008563965559,
-0.04254043474793434,
0.003878162009641528,
0.016488363966345787,
-0.07311145961284637,
0.058596719056367874,
-0.0185875091701746,
0.059576570987701416,
0.0750841274857521,
0.02308039925992489,
0.024173002690076828,
-0.05049140751361847,
-0.23282785713672638,
-0.00835849903523922,
0.007103171665221453,
0.009028932079672813,
-0.012630908749997616,
-0.02816828526556492,
0.047525424510240555,
-0.021743327379226685,
-0.021156825125217438,
-0.030975354835391045,
0.005457600113004446,
0.04763029143214226,
0.011456337757408619,
0.00698047922924161,
-0.01549228373914957,
-0.00891988817602396,
-0.029533620923757553,
-0.06782719492912292,
0.005216927733272314,
0.05174316465854645,
0.08611844480037689,
-0.04711500555276871,
0.009867675602436066,
0.030221939086914062,
0.029059825465083122,
0.011830965988337994,
0.05954338237643242,
0.050766561180353165,
0.03378600627183914,
-0.007084772922098637,
-0.005324499681591988,
0.027672363445162773,
0.012734433636069298,
-0.14127817749977112,
0.08110012114048004,
0.07325872033834457,
0.05146433785557747,
-0.015596511773765087,
-0.04542865976691246,
0.08324333280324936,
0.07737342268228531,
-0.0721263736486435,
0.05539334937930107,
0.016812289133667946,
0.05306236818432808,
0.03008759766817093,
0.0012916817795485258,
-0.03293020278215408,
-0.014877009205520153,
0.0651785284280777,
-0.010183720849454403,
-0.003962110728025436,
-0.02665182389318943,
-0.015154208056628704,
-0.014413055963814259,
-0.03067689947783947,
0.013751368038356304,
0.03518811613321304,
-0.019349129870533943,
0.0185690987855196,
0.06137159839272499,
0.038461167365312576,
-0.049403320997953415,
0.016973178833723068,
-0.019874291494488716,
-0.047540538012981415,
0.039024218916893005,
0.05602927505970001,
-0.03383466973900795,
0.052731484174728394,
0.38788288831710815,
-0.044016528874635696,
0.03333812952041626,
-0.08763748407363892,
-0.024253927171230316,
0.022677870467305183,
-0.0435294508934021,
-0.05773349106311798,
0.0066175516694784164,
-0.03425038605928421,
0.02439386211335659,
0.03449266776442528,
-0.01317012868821621,
0.047026969492435455,
-0.081797294318676,
-0.024988729506731033,
-0.01368717197328806,
0.012793962843716145,
0.0046339090913534164,
-0.02232084982097149,
0.016701074317097664,
-0.08391448110342026,
0.03589677810668945,
0.004088901448994875,
0.05939895287156105,
0.021803272888064384,
0.025368858128786087,
-0.01975119486451149,
0.11254099756479263,
0.0470275841653347,
0.04033058509230614,
0.010688619688153267,
0.008992145769298077,
0.019875889644026756,
0.0010212981142103672,
0.014165328815579414,
-0.024113839492201805,
-0.0200823824852705,
0.014258859679102898,
-0.001831170404329896,
0.045285895466804504,
-0.050682298839092255,
-0.04822149500250816,
-0.0627015233039856,
-0.049831412732601166,
-0.02796761877834797,
0.0729818344116211,
-0.04156853258609772,
-0.01674988679587841,
-0.02589060738682747,
0.010586760938167572,
0.03326568007469177,
0.0007766972412355244,
-0.03680844604969025,
0.018154866993427277,
-0.010091617703437805,
0.02860797382891178,
0.018351472914218903,
-0.06435444951057434,
-0.027919208630919456,
0.026286428794264793,
-0.04661555588245392,
-0.009530717507004738,
0.0009945438941940665,
0.050984036177396774,
0.01847991906106472,
-0.00760448956862092,
-0.001992659643292427,
0.008829154074192047,
-0.015748582780361176,
0.017160998657345772,
0.02061568945646286,
-0.010317106731235981,
-0.03542553633451462,
-0.0430564247071743,
-0.02907595783472061,
0.03057246096432209,
0.007711786311119795,
-0.007356058340519667,
0.038304708898067474,
0.008177637122571468,
0.033367328345775604,
0.008820641785860062,
-0.01644740253686905,
0.008560596965253353,
0.06707074493169785,
-0.07629517465829849,
-0.04165913909673691,
0.026918213814496994,
-0.009147902019321918,
0.006401191931217909,
-0.04112018644809723,
-0.026163533329963684,
-0.06601351499557495,
0.044990263879299164,
-0.05391129478812218,
-0.018182752653956413,
-0.067512147128582,
0.005396571010351181,
-0.009664077311754227,
-0.013817689381539822,
0.12799841165542603,
0.028340917080640793,
-0.03807013854384422,
0.02432999014854431,
0.013098475523293018,
0.0005041543045081198,
0.02690044231712818,
0.04027028754353523,
0.033706583082675934,
0.004005590453743935,
-0.015727480873465538,
0.021488012745976448,
-0.07615238428115845,
0.001493057468906045,
-0.09507603198289871,
0.029811948537826538,
0.04878450930118561,
0.01610228791832924,
0.03736617788672447,
-0.015383156947791576,
0.004341423977166414,
-0.04694814234972,
-0.060783058404922485,
-0.288957804441452,
-0.008771967142820358,
0.002786810975521803,
0.019170710816979408,
0.048802368342876434,
-0.05253613740205765,
-0.028472119942307472,
0.01692379079759121,
0.013749843463301659,
-0.01963571272790432,
0.015819059684872627,
0.027591833844780922,
-0.01237589493393898,
-0.0516258180141449,
0.03671641647815704,
0.04666147753596306,
0.04130968451499939,
-0.027900006622076035,
0.04041387885808945,
0.00959471333771944,
-0.0016844096826389432,
-0.025851832702755928,
-0.005155288148671389,
-0.01011514663696289,
0.05613209679722786,
-0.008500373922288418,
0.11219852417707443,
-0.001585188671015203,
-0.05077285319566727,
0.004957339260727167,
-0.0109227504581213,
0.06249907985329628,
-0.041792672127485275,
-0.08387024700641632,
-0.06792367994785309,
0.034731633961200714,
-0.0015639520715922117,
-0.050679147243499756,
0.020050961524248123,
-0.028260942548513412,
-0.0021125185303390026,
-0.0038394939620047808,
-0.002651497023180127,
-0.09553367644548416,
-0.002018189989030361,
0.010006093420088291,
-0.020966701209545135,
-0.04883507266640663,
-0.0036398679949343204,
0.009954076260328293,
0.0321662463247776,
-0.021250521764159203,
-0.01650039292871952,
-0.027816077694296837,
-0.03101949207484722,
-0.010405794717371464,
-0.00018148055823985487,
0.013362109661102295,
-0.01389484666287899,
0.02280435711145401,
-0.03551465645432472,
0.012632885947823524,
0.00026923042605631053,
0.057799771428108215,
0.009149073623120785,
-0.0732569471001625,
0.039470966905355453,
-0.05485179275274277,
0.03568048030138016,
0.020841063931584358,
0.0014039011439308524,
0.0806521326303482,
-0.05679149553179741,
0.021786343306303024,
0.07473085820674896,
0.0339457206428051,
-0.04009048640727997,
-0.005789137911051512,
-0.06085558980703354,
0.010820307768881321,
0.021214881911873817,
0.024539167061448097,
0.052326664328575134,
-0.05664115399122238,
0.003488321555778384,
0.06663776934146881,
0.011798594146966934,
0.00759284570813179,
0.017190858721733093,
0.07907381653785706,
0.0012026913464069366,
-0.00444458145648241,
-0.020020155236124992,
-0.055616553872823715,
0.028908366337418556,
0.03443104028701782,
-0.2961026728153229,
0.01625054143369198,
0.026307737454771996,
0.07407259941101074,
-0.08385474234819412,
0.04330630227923393,
0.07332360744476318,
-0.053364723920822144,
-0.020770402625203133,
0.07790473848581314,
-0.05640058591961861,
0.014984731562435627,
0.0334024541079998,
0.008283750154078007,
-0.045193590223789215,
-0.0007715069223195314,
0.036210253834724426,
-0.011305062100291252,
-0.0046351212076842785,
-0.036240529268980026,
-0.04126378893852234,
-0.028033314272761345,
0.17348942160606384,
0.03375815227627754,
-0.00005822352250106633,
0.052687421441078186,
-0.029573136940598488,
0.05535594001412392,
-0.03384381905198097,
-0.03281014785170555,
-0.023477688431739807,
0.05552719533443451,
0.06667304039001465,
-0.06032981351017952,
0.022036273032426834,
0.03645985573530197,
-0.01991477608680725,
-0.023345287889242172,
-0.027537405490875244,
0.04017143324017525,
0.03307069465517998,
0.040551792830228806,
-0.049558889120817184,
0.015482725575566292,
0.044589728116989136,
-0.019333653151988983,
-0.011266167275607586,
0.023124653846025467,
-0.015951357781887054,
0.017288928851485252,
-0.0293215773999691,
-0.02527305670082569,
0.03266908973455429,
0.022757891565561295,
-0.003814316587522626,
-0.051117364317178726,
-0.04556826874613762,
-0.009348684921860695,
-0.022272543981671333,
-0.015482542105019093,
0.0688110888004303,
-0.005341405514627695,
-0.03395452722907066,
0.07052464783191681,
0.008279839530587196
] |
PMID:8400 | Complete enzymic digestion of acidic proteins. | Acidic proteins are usually resistant to complete enzymic hydrolysis. The increasing number of "unusual" amino acids, which are unstable to acid hydrolysis, makes it necessary to have a method of enzymic hydrolysis applicable to all proteins. The complete hydrolysis of four acidic proteins by subtilisin plus leucine amino-peptidase plus prolidase followed by carboxypeptidase C, is described. Recoveries of amino acids were in excellent agreement with the expected content from the known sequences. | Complete enzymic digestion of acidic proteins. Acidic proteins are usually resistant to complete enzymic hydrolysis. The increasing number of "unusual" amino acids, which are unstable to acid hydrolysis, makes it necessary to have a method of enzymic hydrolysis applicable to all proteins. The complete hydrolysis of four acidic proteins by subtilisin plus leucine amino-peptidase plus prolidase followed by carboxypeptidase C, is described. Recoveries of amino acids were in excellent agreement with the expected content from the known sequences. | [
-0.06890778988599777,
-0.0034323493018746376,
-0.07550924271345139,
0.054282430559396744,
-0.015760252252221107,
-0.07947883009910583,
0.033621080219745636,
0.04183407500386238,
-0.011636554263532162,
0.01034765224903822,
0.0015584503998979926,
-0.10292574763298035,
-0.01750415936112404,
-0.0106760673224926,
-0.002746125916019082,
-0.003078550100326538,
0.012792211957275867,
0.05336156114935875,
-0.0630069226026535,
-0.06932976096868515,
0.006752905901521444,
0.07517039030790329,
0.06155853345990181,
-0.07720447331666946,
-0.009906910359859467,
0.01194070279598236,
-0.023319248110055923,
-0.030492521822452545,
-0.07880668342113495,
-0.19590072333812714,
0.008031873032450676,
-0.05331182852387428,
0.023522214964032173,
-0.04358075186610222,
-0.006584916729480028,
0.04890882968902588,
-0.005199664272367954,
0.011416570283472538,
0.007094902917742729,
0.06917710602283478,
0.04879244044423103,
0.04429590329527855,
0.01284792460501194,
0.00834839791059494,
0.008169225417077541,
0.029307527467608452,
-0.031182633712887764,
-0.01649688556790352,
0.07653184235095978,
0.0015738420188426971,
0.002261009067296982,
0.007611085195094347,
-0.0031681794207543135,
0.059557411819696426,
-0.004615639336407185,
-0.01930689439177513,
0.01717904396355152,
0.025677403435111046,
-0.014805774204432964,
-0.004155463073402643,
0.0703713521361351,
0.008352085947990417,
-0.10781235247850418,
0.016030186787247658,
0.06738706678152084,
0.04698760062456131,
-0.03160879760980606,
-0.05744292214512825,
0.0558808296918869,
0.09163132309913635,
-0.017618507146835327,
-0.03645789250731468,
-0.020187046378850937,
0.05882417783141136,
-0.02404039166867733,
0.021141091361641884,
0.0015753863845020533,
0.013865187764167786,
-0.016841575503349304,
0.014792609959840775,
0.017318494617938995,
-0.05154085531830788,
0.07689787447452545,
-0.003420659340918064,
0.028911329805850983,
0.018643496558070183,
0.01108031440526247,
0.04211457818746567,
0.04520759359002113,
0.020122243091464043,
-0.003264599246904254,
-0.0007120273658074439,
0.02368605136871338,
0.03434453904628754,
-0.044621046632528305,
0.051061175763607025,
0.06856603175401688,
-0.03467528149485588,
0.035636935383081436,
0.3571940064430237,
-0.07913103699684143,
0.017032301053404808,
0.019052231684327126,
-0.003062913194298744,
-0.05892603471875191,
-0.002179526025429368,
-0.005845321342349052,
0.002220672555267811,
0.012465103529393673,
0.01814114674925804,
-0.03876017406582832,
0.0560174360871315,
0.03914877027273178,
-0.06644131988286972,
-0.008142170496284962,
0.0466003343462944,
-0.023030420765280724,
-0.05108429864048958,
-0.005224419292062521,
0.04303446039557457,
-0.02165786549448967,
0.024694077670574188,
0.01838216558098793,
-0.021458730101585388,
0.0054201544262468815,
0.04272771626710892,
-0.010791908949613571,
0.06052141264081001,
0.09201078861951828,
0.00891097541898489,
-0.004427855368703604,
0.005071320105344057,
0.00562861654907465,
-0.011944584548473358,
0.014841784723103046,
-0.016840839758515358,
0.04890872538089752,
-0.013102293014526367,
0.0361148975789547,
-0.009492955170571804,
-0.05769022926688194,
-0.03600175306200981,
-0.05462150275707245,
-0.053918853402137756,
-0.06580860167741776,
0.07474671304225922,
0.0000890679657459259,
-0.0051564681343734264,
-0.020542632788419724,
-0.010230932384729385,
0.028576722368597984,
0.035174410790205,
0.0601901039481163,
0.02053256891667843,
0.036145344376564026,
0.01965789496898651,
0.0162963904440403,
-0.04408259689807892,
-0.03514162823557854,
-0.01470671035349369,
-0.08597785979509354,
-0.002871590433642268,
0.0044922796078026295,
0.09012987464666367,
0.03888729214668274,
-0.0068801399320364,
-0.0413980633020401,
0.011832082644104958,
-0.003374864812940359,
-0.024851633235812187,
-0.03135208785533905,
-0.038044147193431854,
-0.07060528546571732,
0.0023917315993458033,
-0.02016911655664444,
-0.027202937752008438,
-0.03949746489524841,
-0.009188555181026459,
0.012905159033834934,
-0.0047867451794445515,
0.059349626302719116,
-0.004893238190561533,
-0.052676230669021606,
0.03363548591732979,
0.03845475986599922,
-0.05936257168650627,
-0.07126005738973618,
0.03245903179049492,
-0.002323918044567108,
0.005498854909092188,
-0.029349759221076965,
0.04604480415582657,
-0.1004774197936058,
-0.019444581121206284,
0.015101784840226173,
-0.03022928349673748,
0.0004595604841597378,
-0.013324099592864513,
0.017164461314678192,
0.0008280492620542645,
0.023808451369404793,
0.03633926436305046,
0.009603476151823997,
0.037717655301094055,
0.04201425239443779,
-0.02104618027806282,
0.004247717559337616,
0.0015678193885833025,
0.011286444962024689,
0.010606840252876282,
-0.06059929355978966,
0.051936663687229156,
-0.12390715628862381,
-0.02949528768658638,
-0.051471661776304245,
-0.007108184974640608,
0.03429478034377098,
0.007134283427149057,
0.027934862300753593,
0.01419784501194954,
0.045248907059431076,
-0.06198715418577194,
-0.025124799460172653,
-0.2625637948513031,
0.025388430804014206,
0.00466858921572566,
-0.045019082725048065,
0.10866015404462814,
-0.06531132012605667,
-0.0246904119849205,
0.005611983127892017,
-0.06872036308050156,
-0.01699860766530037,
0.007141557056456804,
0.019262446090579033,
-0.04249625653028488,
-0.039030466228723526,
0.05402624234557152,
0.10319269448518753,
0.025881027802824974,
-0.05654698237776756,
0.0341210663318634,
0.033064618706703186,
-0.017129547894001007,
-0.023174714297056198,
0.06173201650381088,
-0.00042003998532891273,
0.062398433685302734,
-0.008169705979526043,
0.12746365368366241,
-0.02240670844912529,
0.060425885021686554,
0.005008852109313011,
-0.017343729734420776,
0.034898798912763596,
-0.05242893099784851,
-0.040970731526613235,
0.003724724519997835,
-0.033200278878211975,
-0.04263882711529732,
-0.10525856167078018,
0.03744446113705635,
-0.003926836419850588,
0.00039388297591358423,
0.03032940998673439,
-0.05226323381066322,
-0.03914416953921318,
0.00824542436748743,
-0.0201734509319067,
-0.023579252883791924,
0.020466212183237076,
0.02372337132692337,
-0.022901790216565132,
0.04814290255308151,
-0.002476181136444211,
-0.008877519518136978,
0.020265638828277588,
0.006457165814936161,
0.02779361978173256,
-0.04334782436490059,
-0.0050790924578905106,
-0.00485081784427166,
-0.035200782120227814,
-0.029032224789261818,
-0.038648441433906555,
-0.03228098154067993,
0.09045445919036865,
0.010669444687664509,
0.002175495959818363,
-0.009103046730160713,
-0.029895786195993423,
0.016848124563694,
-0.01929997280240059,
-0.0014627352356910706,
0.08040894567966461,
-0.05976596474647522,
0.01987469010055065,
0.022487806156277657,
0.00041542091639712453,
-0.008569663390517235,
-0.04918159544467926,
-0.03874066844582558,
0.00016345175390597433,
-0.025607053190469742,
-0.03682734817266464,
0.057684868574142456,
-0.003992242272943258,
0.02615911141037941,
0.11656747758388519,
0.024560339748859406,
-0.07281093299388885,
-0.05193883553147316,
0.027599694207310677,
0.09187232702970505,
-0.007025896105915308,
-0.052034083753824234,
-0.01730046048760414,
0.023702699691057205,
0.04125460982322693,
-0.26552814245224,
0.07928527891635895,
0.06089243292808533,
0.028084443882107735,
-0.005047396756708622,
0.06004086509346962,
-0.040312375873327255,
-0.043534401804208755,
-0.020276756957173347,
0.05884961038827896,
-0.00452319486066699,
0.018260568380355835,
0.07041149586439133,
-0.0315275676548481,
0.03580121695995331,
-0.018176570534706116,
0.0785457119345665,
-0.05947064608335495,
0.04003084450960159,
-0.016635552048683167,
0.030376523733139038,
-0.005894163157790899,
0.1431787610054016,
0.022798335179686546,
0.0164298415184021,
0.02716086059808731,
-0.01035945676267147,
0.024125222116708755,
-0.015060940757393837,
-0.000935401942115277,
0.010019544512033463,
-0.014179383404552937,
0.04083438962697983,
-0.05597617104649544,
0.041324470192193985,
0.02489597536623478,
-0.0006962933111935854,
0.005506934132426977,
-0.04524306580424309,
0.018350614234805107,
-0.014728770591318607,
-0.05122800171375275,
-0.09845104813575745,
-0.05171744525432587,
0.038226429373025894,
-0.028316734358668327,
-0.028810273855924606,
-0.07443728297948837,
-0.016013750806450844,
-0.04631180688738823,
0.02193293161690235,
-0.07308954000473022,
0.001044288743287325,
-0.01377167459577322,
-0.012996376492083073,
0.07707088440656662,
-0.0460575707256794,
0.009217971004545689,
-0.07040568441152573,
0.004029400646686554,
0.1028442233800888,
0.07408732920885086,
-0.028007322922348976,
0.11520496010780334,
-0.00658641941845417
] |
PMID:8401 | Interpretation and applications of thermal difference spectra of proteins. | The technique of thermal perturbation difference spectroscopy for examining chromophores in proteins has been set on a more acceptable theoretical and experimental foundation. (i) The origins of the thermal effect have been analysed to explain changes in spectral band width and wavelength for simple chromophores in various solvents. Comparison with theoretical curves shows that the effect of heating chromophore solutions is mainly spectral broadening coupled with an almost negligible blue shift. The apparently anomalous behavior of tryptophan and tyrosine in aqueous solvents, where the main effect is a red shift on heating, is traced to hydrogen bonding with water. A model in which tyrosine acts simultaneously as H-donor (Type I) and H-acceptor (Type II) and the later is the more temperature sensitive, is successful in explaining all available data for a variety of solute derivatives and solvents. (ii) The contribution of chromophores in the protein interior has been assessed using polyvinyl alcohol films of differing water content. There models simulate the rigidity and low thermal expansivity of the protein interior and confirm that buried chromophores contribute negligibly if at all to thermal difference spectra. (iii) Curve-fitting procedures have been used for matching protein difference spectra over a wavelength range, to those for mixtures of models, rather than relying as hitherto on data measured at extrema. | Interpretation and applications of thermal difference spectra of proteins. The technique of thermal perturbation difference spectroscopy for examining chromophores in proteins has been set on a more acceptable theoretical and experimental foundation. (i) The origins of the thermal effect have been analysed to explain changes in spectral band width and wavelength for simple chromophores in various solvents. Comparison with theoretical curves shows that the effect of heating chromophore solutions is mainly spectral broadening coupled with an almost negligible blue shift. The apparently anomalous behavior of tryptophan and tyrosine in aqueous solvents, where the main effect is a red shift on heating, is traced to hydrogen bonding with water. A model in which tyrosine acts simultaneously as H-donor (Type I) and H-acceptor (Type II) and the later is the more temperature sensitive, is successful in explaining all available data for a variety of solute derivatives and solvents. (ii) The contribution of chromophores in the protein interior has been assessed using polyvinyl alcohol films of differing water content. There models simulate the rigidity and low thermal expansivity of the protein interior and confirm that buried chromophores contribute negligibly if at all to thermal difference spectra. (iii) Curve-fitting procedures have been used for matching protein difference spectra over a wavelength range, to those for mixtures of models, rather than relying as hitherto on data measured at extrema. | [
-0.09850405901670456,
0.0172815453261137,
0.00625354889780283,
-0.004087076056748629,
0.044500481337308884,
0.011127407662570477,
0.07085175067186356,
0.022550450637936592,
-0.003922766540199518,
-0.04914688318967819,
0.03732225298881531,
-0.05853380262851715,
0.008027403615415096,
0.03325015679001808,
0.0014674417907372117,
-0.018706418573856354,
-0.009784350171685219,
-0.027465874329209328,
-0.0526292584836483,
0.018295226618647575,
-0.004355025943368673,
-0.018322119489312172,
-0.027513565495610237,
-0.01505234930664301,
0.008402752690017223,
0.04800743609666824,
-0.012038346379995346,
0.06029364466667175,
-0.05425232648849487,
-0.24064922332763672,
0.022641904652118683,
-0.00804224144667387,
-0.001246632426045835,
-0.06400221586227417,
0.021138671785593033,
-0.030437756329774857,
0.007219001650810242,
-0.040284208953380585,
-0.06757088005542755,
-0.010909966193139553,
0.06259918957948685,
0.012093045748770237,
0.0017331448616459966,
-0.006974348332732916,
0.011351950466632843,
-0.007924839854240417,
-0.01567034050822258,
0.039628975093364716,
0.020869115367531776,
0.0010905817616730928,
-0.07695118337869644,
-0.01600649021565914,
-0.03309498354792595,
0.03637266904115677,
0.02072925679385662,
0.07613210380077362,
0.04942646622657776,
0.08132898807525635,
-0.018033083528280258,
0.035476651042699814,
0.02129734680056572,
0.048448845744132996,
-0.1069832444190979,
0.07950709015130997,
0.06747039407491684,
0.0030843685381114483,
0.02384411171078682,
-0.0616166926920414,
0.040780529379844666,
0.009526035748422146,
-0.05324322357773781,
0.05602287873625755,
0.012030779384076595,
0.003435982158407569,
0.01743815653026104,
-0.03091130219399929,
-0.03890319541096687,
-0.03240780904889107,
0.009587498381733894,
-0.0022445570211857557,
0.02879779040813446,
-0.010765034705400467,
0.02083147130906582,
-0.03993382677435875,
-0.02094164676964283,
0.021329538896679878,
0.015048317611217499,
-0.01208480354398489,
0.0430167131125927,
0.02105543576180935,
-0.010329051874577999,
-0.06413323432207108,
-0.002394772367551923,
-0.005063157062977552,
-0.05576100945472717,
0.041889164596796036,
0.022062066942453384,
-0.004778173752129078,
0.07036315649747849,
0.3952365815639496,
-0.018185466527938843,
0.008861919865012169,
-0.06628762185573578,
-0.013799554668366909,
0.010812181048095226,
-0.03922659531235695,
-0.0430646575987339,
0.019983936101198196,
0.010649528354406357,
0.014486711472272873,
0.010492402128875256,
-0.011156934313476086,
-0.009941458702087402,
-0.049638859927654266,
-0.08089181035757065,
-0.034010156989097595,
0.012847074307501316,
-0.015535975806415081,
-0.0038472688756883144,
-0.0024633072316646576,
-0.04837813973426819,
0.03216508775949478,
0.05082274600863457,
-0.001733386074192822,
0.015844760462641716,
-0.011958605609834194,
-0.010617073625326157,
0.07643270492553711,
0.03802693262696266,
0.01096865814179182,
0.019205862656235695,
0.016810797154903412,
-0.006003737915307283,
-0.016057614237070084,
0.02083255723118782,
0.029203666374087334,
-0.03244979307055473,
-0.030329788103699684,
0.07608386874198914,
0.0308065302670002,
0.0147556122392416,
-0.020767752081155777,
-0.031777605414390564,
-0.042314931750297546,
-0.03044753521680832,
0.06820011883974075,
-0.019517460837960243,
0.036636024713516235,
-0.07680387794971466,
-0.001199514721520245,
-0.009840979240834713,
0.09068481624126434,
-0.05686861276626587,
0.028342608362436295,
0.020055947825312614,
0.04924637824296951,
-0.03230836242437363,
-0.07384418696165085,
-0.010852296836674213,
-0.003635794622823596,
-0.04592147096991539,
0.027414361014962196,
0.0017506185686215758,
0.043121322989463806,
0.003681344911456108,
-0.05610266700387001,
0.028296731412410736,
0.008036164566874504,
-0.04421738535165787,
-0.03107248991727829,
0.0036822224501520395,
0.01197079848498106,
-0.018874259665608406,
-0.01844026893377304,
-0.04477528855204582,
-0.020933303982019424,
0.01721232943236828,
-0.006066751666367054,
0.0265653096139431,
0.0015643358929082751,
0.013780570589005947,
0.034572526812553406,
0.004164553713053465,
0.048397574573755264,
0.08385012298822403,
-0.015611500479280949,
-0.041447754949331284,
0.07422924786806107,
0.005377126391977072,
0.06834769248962402,
-0.043773215264081955,
-0.030689425766468048,
-0.08091911673545837,
0.06274766474962234,
-0.003303972538560629,
0.012848335318267345,
-0.03992871195077896,
0.0438605435192585,
-0.017382485792040825,
-0.01111646182835102,
0.11886993795633316,
0.015529881231486797,
-0.024687031283974648,
0.027910083532333374,
-0.008770586922764778,
0.01896517165005207,
0.05995685234665871,
0.01801610179245472,
-0.02312517538666725,
-0.018529798835515976,
-0.04845234006643295,
-0.007212758995592594,
-0.11010955274105072,
-0.015601309016346931,
-0.09074667096138,
-0.023549791425466537,
0.054554130882024765,
0.035088568925857544,
0.026351850479841232,
0.028349805623292923,
0.004996903240680695,
-0.06813175976276398,
-0.07374612241983414,
-0.311868816614151,
-0.013985391706228256,
-0.000538777734618634,
-0.02936873584985733,
0.1185736209154129,
-0.04375239089131355,
-0.026794686913490295,
-0.00210024556145072,
0.040242280811071396,
-0.03559207171201706,
0.00913227815181017,
0.04239676147699356,
-0.018048815429210663,
-0.11180514842271805,
0.0356268472969532,
-0.01653280481696129,
0.04733935371041298,
-0.035748451948165894,
0.0348452627658844,
0.07171332091093063,
-0.03624451532959938,
0.041632331907749176,
-0.037409596145153046,
-0.008343677967786789,
0.051267415285110474,
-0.042502954602241516,
0.08502745628356934,
0.00043986219679936767,
0.0020901833195239305,
0.011681395582854748,
-0.07742981612682343,
0.025078395381569862,
-0.07097536325454712,
-0.055847663432359695,
-0.009910513646900654,
0.003858354175463319,
0.05236354470252991,
-0.000268859148491174,
0.03684797137975693,
-0.032607048749923706,
0.043311621993780136,
0.07264535129070282,
-0.00785151869058609,
-0.04134920984506607,
-0.062391623854637146,
-0.022621897980570793,
0.06417607516050339,
-0.043460432440042496,
-0.007704575080424547,
0.03908534348011017,
0.01161931362003088,
-0.01863914541900158,
0.04095080494880676,
-0.033595647662878036,
-0.007235012017190456,
-0.026124270632863045,
-0.004518300294876099,
0.015688255429267883,
0.0021009710617363453,
-0.0027609756216406822,
0.05180833488702774,
0.0021476023830473423,
0.03156983107328415,
0.04253815859556198,
0.010627594776451588,
-0.03323807939887047,
0.04146101698279381,
-0.019771326333284378,
0.09712666273117065,
-0.01855701021850109,
-0.00635119155049324,
0.08786532282829285,
-0.05499710142612457,
0.09779100865125656,
0.04590931534767151,
0.010414495132863522,
-0.035623107105493546,
-0.028215473517775536,
-0.04428033530712128,
0.05868329852819443,
0.033462949097156525,
0.024858329445123672,
0.03297102078795433,
-0.027201540768146515,
0.016502436250448227,
0.030909614637494087,
0.0016983802197501063,
-0.02353561483323574,
0.009698339737951756,
0.005062219221144915,
-0.021617786958813667,
-0.010531418025493622,
0.01445447001606226,
-0.005260775331407785,
0.02395317144691944,
0.029841352254152298,
-0.2501307427883148,
-0.0010525515535846353,
0.02158011868596077,
0.02614937722682953,
-0.04442755877971649,
0.027763303369283676,
0.013224415481090546,
-0.01860102452337742,
-0.06483352929353714,
0.01654825545847416,
-0.0807814821600914,
0.032115042209625244,
0.060297466814517975,
0.023480450734496117,
-0.04502391070127487,
0.013403553515672684,
0.009452592581510544,
-0.046507444232702255,
-0.016378864645957947,
-0.018571633845567703,
0.019979150965809822,
-0.03717673569917679,
0.17375525832176208,
-0.08217420428991318,
0.00045497590326704085,
0.0590132437646389,
0.07253193110227585,
0.05501782149076462,
0.008860573172569275,
0.009777214378118515,
-0.030806124210357666,
0.014070711098611355,
0.04261701554059982,
-0.0536210872232914,
0.02852560579776764,
0.038204602897167206,
-0.00045239541213959455,
-0.013389895670115948,
0.0266630370169878,
-0.025708945468068123,
0.03251016139984131,
-0.054371435195207596,
-0.0678754597902298,
-0.02566526085138321,
0.03597821667790413,
-0.0031902503687888384,
-0.04414651542901993,
0.010707367211580276,
-0.02273917943239212,
-0.021212629973888397,
-0.011113803833723068,
-0.016091782599687576,
-0.012239733710885048,
0.03585952892899513,
0.009927459061145782,
0.03782305493950844,
-0.06865227967500687,
-0.014511552639305592,
0.004025684203952551,
0.01571183279156685,
0.00640680268406868,
-0.008686404675245285,
-0.05149270221590996,
0.044660381972789764,
0.02085588313639164
] |
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.