id
stringlengths 7
10
| title
stringlengths 8
354
| content
stringlengths 91
4.1k
| contents
stringlengths 164
4.24k
| embedding
listlengths 384
384
|
|---|---|---|---|---|
PMID:18467
|
Purification of human erythropoietin.
|
Human erythropoietin, derived from urine of patients with aplastic anemia, has been purified to apparent homogeneity. The seven-step procedure, which included ion exchange chromatography, ethanol precipitation, gel filtration, and adsorption chromatography, yielded a preparation with a potency of 70,400 units/mg of protein in 21% yield. This represents a purification factor of 930. The purified hormone has a single electrophoretic component in polyacrylamide gels at pH 9, in the presence of sodium dodecylsulfate at pH 7, and in the presence of Triton X-100 at pH 6. Two fractions of the same potency and molecular size, by sodium dodecyl sulfate gel electrophoresis, but differing slightly in mobility at pH 9, were obtained at the last step of fractionation. The nature of the difference between these two components is not yet understood.
|
Purification of human erythropoietin. Human erythropoietin, derived from urine of patients with aplastic anemia, has been purified to apparent homogeneity. The seven-step procedure, which included ion exchange chromatography, ethanol precipitation, gel filtration, and adsorption chromatography, yielded a preparation with a potency of 70,400 units/mg of protein in 21% yield. This represents a purification factor of 930. The purified hormone has a single electrophoretic component in polyacrylamide gels at pH 9, in the presence of sodium dodecylsulfate at pH 7, and in the presence of Triton X-100 at pH 6. Two fractions of the same potency and molecular size, by sodium dodecyl sulfate gel electrophoresis, but differing slightly in mobility at pH 9, were obtained at the last step of fractionation. The nature of the difference between these two components is not yet understood.
|
[
-0.0587269626557827,
0.021842243149876595,
-0.04215379059314728,
0.008846865966916084,
-0.005101115442812443,
-0.027008241042494774,
0.09106770157814026,
0.013854441232979298,
-0.05367737263441086,
0.04156272113323212,
-0.014941448345780373,
-0.0778173878788948,
-0.004650562070310116,
0.036052580922842026,
-0.03359939530491829,
0.04332061856985092,
0.028231769800186157,
0.0182733666151762,
-0.08568959683179855,
0.04433735832571983,
0.013957804068922997,
-0.10168327391147614,
0.0019711204804480076,
-0.005816757678985596,
0.03907964006066322,
0.04984719678759575,
-0.059239715337753296,
-0.05062803253531456,
-0.003813134739175439,
-0.18640699982643127,
0.021656787022948265,
0.0280951876193285,
0.05808735266327858,
-0.053058452904224396,
0.002836306346580386,
0.03608711436390877,
-0.035206351429224014,
-0.016695372760295868,
-0.017835848033428192,
0.052949778735637665,
0.062011200934648514,
0.03129322826862335,
-0.05723138153553009,
0.011091431602835655,
0.054800327867269516,
0.04412676393985748,
-0.05871821567416191,
0.013078696094453335,
0.07557060569524765,
0.04602004960179329,
0.03243156895041466,
0.06708502769470215,
0.05415700376033783,
0.017253754660487175,
0.021815195679664612,
-0.032302647829055786,
0.009166885167360306,
0.04094233363866806,
-0.02791900560259819,
0.023836255073547363,
-0.04524883255362511,
0.05081300064921379,
-0.13093234598636627,
0.0845821350812912,
0.05204655975103378,
0.004960639867931604,
-0.0061738272197544575,
-0.045471154153347015,
0.054393380880355835,
0.005425723269581795,
0.006807486992329359,
0.08056822419166565,
0.00031426973873749375,
0.07152120023965836,
-0.08975448459386826,
-0.012094870209693909,
0.03173626959323883,
-0.06235590577125549,
0.027079472318291664,
0.03529541566967964,
-0.010391061194241047,
0.03877256438136101,
0.01781063713133335,
0.020955678075551987,
0.02612522803246975,
-0.020163698121905327,
0.01806695945560932,
0.02072506584227085,
-0.033437471836805344,
0.04557709023356438,
0.03233979642391205,
-0.024669911712408066,
-0.024970557540655136,
0.04205310717225075,
-0.05519375205039978,
-0.008675280958414078,
-0.02451137825846672,
-0.022802874445915222,
0.0378391295671463,
0.39445260167121887,
-0.031567126512527466,
0.0373932383954525,
-0.004714067094027996,
-0.03213833272457123,
0.021928230300545692,
-0.0049845390021800995,
-0.0190927404910326,
0.054535239934921265,
0.00041098130168393254,
0.029955852776765823,
-0.024023886770009995,
-0.02038257196545601,
0.039243828505277634,
-0.03919548541307449,
0.025097258388996124,
-0.006405532825738192,
0.005793129093945026,
-0.020124759525060654,
0.034713223576545715,
0.07119782269001007,
-0.02143465168774128,
-0.03183400258421898,
0.011735055595636368,
-0.050454575568437576,
-0.01727185770869255,
-0.009489103220403194,
0.006171716842800379,
0.09428392350673676,
0.04157231003046036,
0.007645597215741873,
0.05636325106024742,
-0.010449085384607315,
-0.036391742527484894,
0.005037063732743263,
-0.013153642416000366,
-0.0025715839583426714,
0.013955194503068924,
-0.06318610161542892,
-0.02750302292406559,
-0.012677693739533424,
0.0030141249299049377,
0.00877097062766552,
-0.03782038763165474,
-0.08472038060426712,
-0.01750820502638817,
0.07157012075185776,
0.03548465296626091,
0.0020610629580914974,
0.016887910664081573,
-0.020351484417915344,
-0.008090857416391373,
0.08670198172330856,
-0.028254499658942223,
0.03155108168721199,
-0.011919045820832253,
0.03384995833039284,
-0.018282756209373474,
-0.03930429369211197,
0.009728124365210533,
0.013038253411650658,
-0.005488029681146145,
-0.0014675587881356478,
-0.04978906735777855,
0.06633685529232025,
0.006023924332112074,
-0.018940282985568047,
-0.05198416858911514,
-0.014346180483698845,
0.02333126589655876,
0.03128141909837723,
0.02914457768201828,
-0.015398870222270489,
-0.04323798045516014,
0.045553989708423615,
-0.030456697568297386,
-0.009194382466375828,
0.038993947207927704,
0.010919244959950447,
0.05725447088479996,
0.01192255225032568,
-0.017265640199184418,
-0.010678164660930634,
-0.039180465042591095,
0.01335751824080944,
0.02018737606704235,
-0.09099587053060532,
-0.04009191691875458,
0.05079572647809982,
0.009451710619032383,
0.035571351647377014,
-0.04934827238321304,
0.06077825650572777,
-0.03473688289523125,
0.026823049411177635,
-0.008688116446137428,
-0.034026212990283966,
-0.04894961789250374,
-0.020502636209130287,
-0.011958032846450806,
-0.030016500502824783,
0.09647347778081894,
-0.01322480570524931,
0.027564268559217453,
0.035941556096076965,
-0.026809552684426308,
0.044691432267427444,
0.0499875545501709,
-0.012714916840195656,
0.04032224416732788,
0.04435516521334648,
-0.0033789968583732843,
0.05650656297802925,
-0.10782480239868164,
0.018774837255477905,
0.012723584659397602,
0.04069073498249054,
0.027872508391737938,
-0.027544083073735237,
0.09546830505132675,
0.016500230878591537,
0.016027698293328285,
-0.0708254873752594,
-0.06799247115850449,
-0.2958047688007355,
0.0096975676715374,
0.008051760494709015,
-0.03586764633655548,
0.04953913018107414,
-0.06285736709833145,
-0.0010201038094237447,
0.0037339203990995884,
-0.0037512751296162605,
-0.040217041969299316,
0.10099470615386963,
0.11122431606054306,
-0.021927662193775177,
-0.030852841213345528,
-0.005047450307756662,
0.008788282983005047,
0.03975358232855797,
-0.01654413528740406,
0.03854995220899582,
0.0327414907515049,
-0.008765464648604393,
0.0019855170976370573,
0.02007957547903061,
0.09410899132490158,
0.008788477629423141,
-0.002370239235460758,
0.09582482278347015,
0.0058715152554214,
-0.004009055905044079,
-0.0024556349962949753,
0.009189249016344547,
0.05943462625145912,
-0.006756407208740711,
-0.13991858065128326,
-0.03220104053616524,
-0.03608902543783188,
-0.05056123808026314,
-0.03481442481279373,
-0.019279025495052338,
-0.02099739760160446,
-0.08254007995128632,
-0.03278598561882973,
0.0052764094434678555,
-0.05675380304455757,
-0.05057542771100998,
-0.015533913858234882,
-0.02473711222410202,
0.045101866126060486,
-0.008772834204137325,
-0.02497309073805809,
0.0984337255358696,
-0.03640482574701309,
0.05364586412906647,
-0.02862166427075863,
-0.025137750431895256,
0.01883438043296337,
-0.07803409546613693,
0.027454620227217674,
-0.08949171751737595,
-0.009969453327357769,
0.019285669550299644,
-0.00011128743790322915,
-0.017759259790182114,
0.012015391141176224,
-0.004333721939474344,
-0.007408048026263714,
0.03796800598502159,
0.017111139371991158,
0.05790291726589203,
0.013292820192873478,
-0.01455147098749876,
0.07132058590650558,
-0.035483915358781815,
0.0433214008808136,
-0.021220868453383446,
0.013362160883843899,
-0.05617823451757431,
-0.011440438218414783,
-0.07717886567115784,
0.024895498529076576,
0.01803494617342949,
-0.030832286924123764,
0.0410538986325264,
0.03696506842970848,
0.03578905761241913,
0.03331921994686127,
0.007273286115378141,
0.030475899577140808,
0.05616708844900131,
0.02532426454126835,
-0.02013266459107399,
-0.017536472529172897,
-0.03833865374326706,
-0.053752098232507706,
-0.005800222046673298,
0.0153672369197011,
-0.24913085997104645,
-0.01213585864752531,
0.020965421572327614,
0.011405572295188904,
0.004910219460725784,
-0.01702895015478134,
0.025136571377515793,
-0.03734461963176727,
0.0010175468632951379,
0.021758124232292175,
-0.015606737695634365,
-0.002009135205298662,
0.08748354017734528,
-0.05548623204231262,
0.004035980440676212,
0.04407753422856331,
0.06551807373762131,
0.007125230506062508,
-0.03776725381612778,
-0.0025898865424096584,
-0.00014135298260953277,
-0.015140987001359463,
0.10764620453119278,
-0.03694261983036995,
-0.0408489890396595,
0.004523120820522308,
0.016076693311333656,
0.019806064665317535,
-0.11994753032922745,
-0.024389103055000305,
0.0122065469622612,
0.025503123179078102,
0.0070722512900829315,
-0.02768002450466156,
-0.01394700538367033,
0.022802580147981644,
0.013179374858736992,
-0.02018664963543415,
0.03402549400925636,
0.007218313403427601,
-0.0854777917265892,
-0.018053125590085983,
-0.0977514311671257,
-0.013768463395535946,
0.07432252168655396,
-0.039416007697582245,
-0.04539330676198006,
-0.0660710409283638,
0.01760932430624962,
-0.008746309205889702,
-0.035479430109262466,
-0.001453392906114459,
0.03938259184360504,
0.005482019856572151,
-0.017889101058244705,
0.022223258391022682,
-0.022390929982066154,
-0.0067301345989108086,
-0.03363805636763573,
-0.07558048516511917,
-0.0349198542535305,
-0.01025590393692255,
-0.03119196929037571,
0.06426726281642914,
0.0015823590802028775
] |
PMID:18468
|
Kinetic mechanism of tRNA nucleotidyltransferase from Escherichia coli.
|
A kinetic analysis of the incorporation of AMP into tRNA lacking the 3'-terminal residue by tRNA nucleotidyltransferase (EC 2.2.7.25) from Escherichia coli is presented. Initial velocity studies demonstrate that the mechanism is sequential and that high concentrations of tRNA give rise to substrate inhibition which is noncompetitive with respect to ATP. In addition, the substrate inhibition is more pronounced in the presence of pyrophosphate, which suggests the formation of an inhibitory enzyme-pyrophosphate-tRNA complex. Noncompetitive product inhibition is observed between all possible pairs of substrates and products. ADP and alpha,beta-methylene adenosine triphosphate are competitive dead end inhibitors of ATP, while the latter is a noncompetitive dead end inhibitor of the tRNA substrate. A nonrapid equilibrium random mechanism is proposed which is consistent with these data and offers an explanation for the noncompetitive substrate inhibition by tRNA.
|
Kinetic mechanism of tRNA nucleotidyltransferase from Escherichia coli. A kinetic analysis of the incorporation of AMP into tRNA lacking the 3'-terminal residue by tRNA nucleotidyltransferase (EC 2.2.7.25) from Escherichia coli is presented. Initial velocity studies demonstrate that the mechanism is sequential and that high concentrations of tRNA give rise to substrate inhibition which is noncompetitive with respect to ATP. In addition, the substrate inhibition is more pronounced in the presence of pyrophosphate, which suggests the formation of an inhibitory enzyme-pyrophosphate-tRNA complex. Noncompetitive product inhibition is observed between all possible pairs of substrates and products. ADP and alpha,beta-methylene adenosine triphosphate are competitive dead end inhibitors of ATP, while the latter is a noncompetitive dead end inhibitor of the tRNA substrate. A nonrapid equilibrium random mechanism is proposed which is consistent with these data and offers an explanation for the noncompetitive substrate inhibition by tRNA.
|
[
-0.11987514048814774,
-0.001234404044225812,
-0.044989559799432755,
0.015209050849080086,
-0.017895478755235672,
0.027924638241529465,
0.020142357796430588,
0.009054291062057018,
-0.022513454779982567,
-0.0010731291258707643,
0.04620816931128502,
-0.04842622950673103,
0.019903553649783134,
0.011742251925170422,
-0.05236267298460007,
-0.01005301158875227,
-0.02416878193616867,
0.01736062951385975,
0.040805455297231674,
0.005618568044155836,
0.031218791380524635,
0.015067366883158684,
0.008783497847616673,
0.0021339065860956907,
0.04123426228761673,
0.07278229296207428,
0.018054155632853508,
0.01906755566596985,
-0.05479966476559639,
-0.21233749389648438,
0.01585645228624344,
-0.035301465541124344,
-0.02709769271314144,
-0.07009901851415634,
-0.009658384136855602,
0.028352178633213043,
-0.059893250465393066,
-0.01159736979752779,
-0.021962709724903107,
0.03332451730966568,
-0.019633468240499496,
0.05230337753891945,
-0.012620367109775543,
-0.02072662115097046,
0.031995177268981934,
-0.031639669090509415,
-0.05359520763158798,
-0.01865059696137905,
0.057281456887722015,
0.03356633707880974,
-0.0936022698879242,
-0.0010550699662417173,
-0.024589676409959793,
-0.012476974166929722,
-0.03393130749464035,
-0.001209778361953795,
0.03737740218639374,
0.05289926752448082,
-0.003285748418420553,
-0.008066348731517792,
0.050835561007261276,
0.030740296468138695,
-0.11763031035661697,
0.07914223521947861,
0.085531085729599,
0.01738518849015236,
0.06849387288093567,
0.04162684082984924,
0.029413916170597076,
0.045772626996040344,
-0.037278056144714355,
0.026387860998511314,
0.009163609705865383,
0.04130490496754646,
0.033599745482206345,
0.03012184053659439,
0.023702561855316162,
0.011157284490764141,
0.10963201522827148,
0.030518651008605957,
-0.0000410610118706245,
0.009715567342936993,
0.012735956348478794,
-0.00885519664734602,
-0.058202970772981644,
0.01798272505402565,
0.03694617748260498,
-0.03878025338053703,
-0.015167386271059513,
0.012823715806007385,
0.017148885875940323,
-0.036234524101018906,
-0.0020693435799330473,
-0.02761754021048546,
-0.03395766019821167,
0.04738311097025871,
0.048477903008461,
-0.039860304445028305,
0.05714437738060951,
0.3740919530391693,
-0.00027505087200552225,
0.0521552599966526,
-0.05859915912151337,
0.009972264058887959,
0.021000638604164124,
-0.02259022928774357,
-0.01515363808721304,
-0.029632452875375748,
0.0007077658083289862,
0.0729685127735138,
0.061404965817928314,
0.02643999457359314,
-0.007766800466924906,
-0.05511213839054108,
0.01406808290630579,
0.04168512672185898,
-0.004533409606665373,
0.021456867456436157,
-0.06876857578754425,
0.0018368359887972474,
-0.05044825002551079,
-0.03487156704068184,
0.016672564670443535,
-0.004393159411847591,
-0.006609492003917694,
0.012739703059196472,
-0.045513372868299484,
0.11213886737823486,
0.06154809147119522,
0.014625169336795807,
0.09895423799753189,
-0.005625973921269178,
0.00008336328028235584,
0.007999762892723083,
0.042091015726327896,
-0.029625115916132927,
-0.03625991567969322,
-0.037854116410017014,
0.007332094945013523,
-0.0489545613527298,
-0.017205769196152687,
-0.059664301574230194,
-0.04402272403240204,
-0.09262937307357788,
-0.012597741559147835,
0.07049146294593811,
-0.007611843291670084,
0.046479884535074234,
-0.035729099065065384,
0.025750821456313133,
-0.00420436542481184,
0.016574228182435036,
-0.013538200408220291,
-0.014558237977325916,
-0.032309286296367645,
0.03827992454171181,
-0.027995413169264793,
-0.019626667723059654,
0.007712692487984896,
0.015783512964844704,
-0.07529131323099136,
-0.03316003456711769,
0.0382491797208786,
0.0592905692756176,
-0.006312109064310789,
-0.025359557941555977,
0.008287588134407997,
-0.01874253712594509,
0.028590736910700798,
-0.02489699423313141,
-0.027834560722112656,
-0.034091245383024216,
-0.03683600574731827,
-0.05205236002802849,
-0.044399864971637726,
-0.004148984793573618,
0.005799386650323868,
0.033903107047080994,
0.025303829461336136,
0.01988360844552517,
0.00803325790911913,
0.008038855157792568,
0.00555080734193325,
0.022405268624424934,
0.06846221536397934,
0.0003604323719628155,
-0.041816193610429764,
-0.0005858027143403888,
0.011427808552980423,
0.0206883754581213,
-0.019957831129431725,
-0.017614949494600296,
-0.11117900162935257,
0.05355331301689148,
-0.06968928128480911,
-0.07715122401714325,
0.04215662553906441,
0.06320054829120636,
0.018474793061614037,
0.05716628581285477,
0.06292708218097687,
0.06489618867635727,
-0.00016254132788162678,
0.07523512095212936,
-0.051321592181921005,
-0.06021978706121445,
-0.028235578909516335,
0.011847514659166336,
0.005678100511431694,
0.04733830690383911,
-0.061898373067379,
-0.035312552005052567,
-0.09626022726297379,
0.004640849772840738,
-0.06516531854867935,
0.0019256540108472109,
-0.019219279289245605,
0.06916987895965576,
0.07504994422197342,
0.011862537823617458,
-0.022410370409488678,
-0.05162039026618004,
-0.02054070122539997,
-0.2903926968574524,
-0.029724659398198128,
0.030570415779948235,
0.0615660585463047,
0.05509122461080551,
-0.013441147282719612,
-0.007917207665741444,
0.018551912158727646,
-0.007456790190190077,
-0.008251884952187538,
0.04607371240854263,
0.06167136877775192,
-0.005337425973266363,
-0.03895961865782738,
-0.030881516635417938,
0.018019163981080055,
-0.012791949324309826,
-0.024574194103479385,
0.009560422040522099,
0.017530012875795364,
0.04676932469010353,
-0.01978776417672634,
-0.052832771092653275,
-0.06669071316719055,
0.018795756623148918,
0.013043169863522053,
0.08465643972158432,
-0.019691292196512222,
0.030685940757393837,
0.06880003213882446,
-0.007429984398186207,
0.08118082582950592,
-0.027853094041347504,
-0.09929891675710678,
-0.016631247475743294,
0.011966626159846783,
-0.026017244905233383,
-0.05194287747144699,
0.06113332509994507,
-0.013390871696174145,
-0.02112831547856331,
0.013030679896473885,
-0.0250385869294405,
-0.06242622062563896,
-0.015727071091532707,
-0.01303911954164505,
0.0017033073818311095,
-0.011226528324186802,
0.028856191784143448,
-0.025625968351960182,
0.0458940751850605,
0.06456191837787628,
0.026733286678791046,
-0.015666835010051727,
0.01770137995481491,
-0.0030444744043052197,
-0.03245404735207558,
0.0071740951389074326,
0.006135813891887665,
0.015311947092413902,
-0.0007197854574769735,
0.031284183263778687,
-0.0393020398914814,
0.0508761927485466,
0.015149552375078201,
-0.03126879781484604,
-0.0025821439921855927,
0.018935061991214752,
0.0811033844947815,
0.010197360999882221,
0.0033430440817028284,
0.09502305835485458,
0.011522048152983189,
0.013229245319962502,
0.01931665651500225,
-0.0006120321922935545,
-0.042839739471673965,
-0.08449899405241013,
-0.059771761298179626,
-0.001705875270999968,
0.009855223819613457,
-0.04912241920828819,
0.055063825100660324,
-0.03989455848932266,
-0.038635388016700745,
0.008067036978900433,
0.04398420825600624,
-0.0593000091612339,
0.025761669501662254,
0.0275995135307312,
-0.010670565068721771,
-0.008954454213380814,
-0.024790659546852112,
-0.020865337923169136,
0.03308739513158798,
0.055095937103033066,
-0.27064210176467896,
-0.06172174587845802,
0.0062625594437122345,
0.004193089436739683,
-0.02317696623504162,
0.02832498960196972,
0.06268981099128723,
-0.039315856993198395,
-0.03743906691670418,
0.030859339982271194,
0.006385059095919132,
0.03301367908716202,
0.00605201069265604,
0.07232087105512619,
0.007180548273026943,
-0.006767996121197939,
0.10632065683603287,
-0.047256410121917725,
0.03211190924048424,
-0.019758440554142,
-0.026452727615833282,
-0.034811604768037796,
0.17065687477588654,
0.00874020904302597,
0.010195107199251652,
0.003489087102934718,
-0.005611891392618418,
0.0511421263217926,
-0.023400723934173584,
-0.056859277188777924,
-0.057184409350156784,
0.020339490845799446,
0.07215291261672974,
-0.06690528988838196,
0.0157428327947855,
0.051666587591171265,
0.03258591890335083,
0.012260740622878075,
-0.05659100413322449,
-0.006612102501094341,
-0.006784774363040924,
0.029764341190457344,
-0.044423725455999374,
-0.023337623104453087,
0.066926971077919,
-0.09421898424625397,
-0.05361250415444374,
-0.07484626770019531,
0.0168461874127388,
0.022234715521335602,
-0.008095690980553627,
0.00661584734916687,
0.007923896424472332,
-0.025542421266436577,
0.026052424684166908,
-0.01634608395397663,
-0.02060682885348797,
-0.017014462500810623,
0.005514047108590603,
-0.01658593863248825,
-0.02072995901107788,
-0.002627552719786763,
-0.018766095861792564,
0.09429077059030533,
-0.011252278462052345
] |
PMID:18469
|
Human thymidylate kinase. Purification, characterization, and kinetic behavior of the thymidylate kinase derived from chronic myelocytic leukemia.
|
Thymidylate kinase derived from the blast cells of human chronic myelocytic leukemia was purified 2186-fold to near homogeneity by means of alcohol precipitation, alumina-Cgamma gel fractionation, calcium phosphate gel fraction, ultrafiltration, and affinity column chromatography. The molecular weight was estimated by glycerol gradient centrifugation to be 50,000. This enzyme had an optimal activity at pH 7.1 and required a divalent cation in order to catalyze the reaction. Mg2+ and Mn2+ were found to be the preferential divalent cations. The activation energy was estimated to be 19.1 kcal/mol at pH 7.2. Initial velocity study suggested that the reaction followed a sequential mechanism. Mg2+ ATP had a Km of 0.25 mM and dTMP had a Km of 40 micrometer. The enzyme was unstable even at 4 degrees. In the presence of ATP or dTMP the enzyme maintained its activity. Purine triphosphate nucleosides were found to be better phosphate donors than the pyrimidine triphosphate nucleosides. ATP and dATP had a lower Km and a higher Vmax than GTP and dGTP. dTMP was the only preferred phosphate receptor among all the monophosphate nucleotides tested dTTP and IdUTP competed with both substrates and inhibited the reaction with a Ki of 0.75 mM and 1.1 mM, respectively.
|
Human thymidylate kinase. Purification, characterization, and kinetic behavior of the thymidylate kinase derived from chronic myelocytic leukemia. Thymidylate kinase derived from the blast cells of human chronic myelocytic leukemia was purified 2186-fold to near homogeneity by means of alcohol precipitation, alumina-Cgamma gel fractionation, calcium phosphate gel fraction, ultrafiltration, and affinity column chromatography. The molecular weight was estimated by glycerol gradient centrifugation to be 50,000. This enzyme had an optimal activity at pH 7.1 and required a divalent cation in order to catalyze the reaction. Mg2+ and Mn2+ were found to be the preferential divalent cations. The activation energy was estimated to be 19.1 kcal/mol at pH 7.2. Initial velocity study suggested that the reaction followed a sequential mechanism. Mg2+ ATP had a Km of 0.25 mM and dTMP had a Km of 40 micrometer. The enzyme was unstable even at 4 degrees. In the presence of ATP or dTMP the enzyme maintained its activity. Purine triphosphate nucleosides were found to be better phosphate donors than the pyrimidine triphosphate nucleosides. ATP and dATP had a lower Km and a higher Vmax than GTP and dGTP. dTMP was the only preferred phosphate receptor among all the monophosphate nucleotides tested dTTP and IdUTP competed with both substrates and inhibited the reaction with a Ki of 0.75 mM and 1.1 mM, respectively.
|
[
-0.0983104258775711,
-0.02544669434428215,
-0.04004179686307907,
-0.03502088412642479,
-0.03936111927032471,
-0.02487114444375038,
0.037101734429597855,
0.02294379286468029,
-0.029138637706637383,
-0.029730601236224174,
0.04209146648645401,
-0.07809371501207352,
0.042956456542015076,
-0.009307020343840122,
-0.05533942952752113,
-0.007699787151068449,
0.007153473328799009,
0.0006936450372450054,
-0.07129404693841934,
-0.01512950100004673,
-0.054261866956949234,
-0.057538215070962906,
-0.014802009798586369,
-0.01138059888035059,
0.030923403799533844,
0.06627051532268524,
0.00810119416564703,
0.03452308848500252,
-0.004126240964978933,
-0.18822616338729858,
0.055779941380023956,
-0.01626400835812092,
-0.015087820589542389,
-0.03367462381720543,
0.0028073580469936132,
-0.019683238118886948,
-0.021079856902360916,
-0.03297632187604904,
-0.03266003727912903,
0.04079066216945648,
0.02885599061846733,
-0.0011655607959255576,
-0.015601878985762596,
0.039264485239982605,
0.03694204241037369,
-0.03482694923877716,
-0.06560777127742767,
0.0048088571056723595,
0.0536058284342289,
0.0425061360001564,
-0.011639071628451347,
0.027555763721466064,
0.03284510597586632,
0.058702293783426285,
-0.004358889535069466,
-0.04655645415186882,
0.03979657217860222,
0.05444137379527092,
0.0436616986989975,
0.04450109973549843,
0.019246943295001984,
0.020990993827581406,
-0.11485368013381958,
0.025148905813694,
0.034434717148542404,
-0.004202276933938265,
0.017034588381648064,
-0.0027822733391076326,
0.04218807443976402,
0.017031798139214516,
-0.060034558176994324,
0.07195891439914703,
0.046997953206300735,
0.03585609421133995,
-0.019163360819220543,
-0.025249721482396126,
0.016028499230742455,
-0.044947464019060135,
0.10648414492607117,
-0.008075820282101631,
-0.024350691586732864,
-0.029654569923877716,
-0.04778570309281349,
-0.02039518766105175,
-0.0432492159307003,
0.035802505910396576,
0.010677265003323555,
-0.018372802063822746,
-0.01778372935950756,
0.05703894421458244,
-0.005307346116751432,
-0.0379411019384861,
0.02284282073378563,
0.001980660017579794,
-0.0996062234044075,
0.06844907253980637,
0.05949893221259117,
0.0195221696048975,
0.0435706190764904,
0.3741648197174072,
0.015137560665607452,
0.09472203999757767,
-0.03479311987757683,
-0.0016117653576657176,
-0.03425292298197746,
-0.04865462705492973,
-0.022360488772392273,
0.036089424043893814,
0.037901122123003006,
0.0018537804717198014,
0.03524453565478325,
-0.018854014575481415,
0.008979017846286297,
-0.0579398013651371,
-0.018391991034150124,
0.019246933981776237,
-0.013200717978179455,
-0.035655658692121506,
-0.016104500740766525,
0.07607970386743546,
-0.007553084287792444,
-0.021638276055455208,
0.021023044362664223,
-0.006378652062267065,
-0.01725272461771965,
0.016106925904750824,
-0.025224197655916214,
0.0645890161395073,
0.11766844987869263,
0.0032015300821512938,
0.08808184415102005,
0.04622102156281471,
-0.0419614352285862,
0.028197413310408592,
0.0631195679306984,
-0.014007058925926685,
-0.031244497746229172,
-0.08105319738388062,
0.051216624677181244,
-0.02851097658276558,
0.0000035051716622547247,
-0.052340809255838394,
-0.09422136843204498,
-0.07177990674972534,
-0.03304243087768555,
0.0624072328209877,
-0.024925194680690765,
0.05135040730237961,
0.014696291647851467,
0.006505176890641451,
0.03421273082494736,
0.08109582215547562,
-0.00488807400688529,
0.0611199215054512,
-0.015151597559452057,
0.0338054895401001,
-0.008180806413292885,
-0.012067821808159351,
-0.015921739861369133,
-0.008238743990659714,
-0.02451002597808838,
-0.005850907415151596,
-0.009828205220401287,
0.07710334658622742,
-0.04590984061360359,
-0.061184875667095184,
-0.04785756394267082,
0.014305169694125652,
-0.008654418401420116,
-0.011793333105742931,
0.02320007048547268,
-0.040838342159986496,
-0.015512875281274319,
-0.01627597026526928,
-0.031191540881991386,
-0.006067625712603331,
-0.0016471470007672906,
0.04816070571541786,
0.05557321757078171,
-0.0062051257118582726,
0.05678999423980713,
-0.008823965676128864,
-0.05049879476428032,
0.03777150809764862,
0.03913900628685951,
-0.010011548176407814,
-0.06175192818045616,
0.019002823159098625,
0.005615829024463892,
0.04277172312140465,
0.00048518742551095784,
0.030865319073200226,
-0.018685832619667053,
0.008408697322010994,
-0.03684437274932861,
-0.06034507602453232,
-0.015487642027437687,
0.03999175503849983,
0.009462938643991947,
-0.0073454659432172775,
0.08148066699504852,
0.004324955865740776,
-0.030880756676197052,
0.06614451110363007,
-0.048858918249607086,
0.036494482308626175,
-0.018187105655670166,
0.013183221220970154,
0.07094982266426086,
0.02394259348511696,
0.027995048090815544,
0.008792116306722164,
-0.06669674068689346,
-0.016852721571922302,
-0.050157591700553894,
0.033809222280979156,
0.04083400219678879,
-0.007615481503307819,
0.05369890108704567,
-0.01766873151063919,
0.02942509576678276,
-0.02442334033548832,
-0.07958735525608063,
-0.27019616961479187,
-0.05387673154473305,
0.048480041325092316,
0.0038475915789604187,
0.055907223373651505,
-0.04706055670976639,
-0.013188960961997509,
0.009862133301794529,
-0.00890717189759016,
-0.06923901289701462,
0.08112676441669464,
0.09566999971866608,
-0.014509562402963638,
-0.06182486191391945,
-0.04002224653959274,
0.03968023136258125,
-0.028072161599993706,
0.03535880148410797,
0.024816719815135002,
-0.06520173698663712,
0.03658423572778702,
-0.028414102271199226,
-0.01884784735739231,
0.0288133155554533,
0.020896747708320618,
0.0037378731649369,
0.07869601249694824,
-0.003720412263646722,
-0.003761064726859331,
-0.015306313522160053,
0.0295301154255867,
-0.003919836599379778,
-0.04869193211197853,
-0.08084501326084137,
-0.04747534915804863,
-0.03948581591248512,
-0.03137233480811119,
-0.0713542029261589,
-0.020962441340088844,
-0.02848106063902378,
-0.04960748180747032,
-0.05667268484830856,
0.0007217451930046082,
-0.055036772042512894,
0.01885553076863289,
0.028828421607613564,
0.031504061073064804,
0.038435325026512146,
-0.013403067365288734,
0.0038174011278897524,
0.018526557832956314,
-0.01863749697804451,
0.048179030418395996,
-0.06752617657184601,
-0.005120173096656799,
-0.010770417749881744,
-0.053679127246141434,
0.028457311913371086,
-0.04893612861633301,
-0.01942143402993679,
-0.03912888839840889,
0.018688036128878593,
-0.046865832060575485,
0.05929923802614212,
-0.029371140524744987,
-0.011077691800892353,
0.07824356108903885,
-0.010361617431044579,
0.08759719133377075,
-0.059912651777267456,
0.0005540862330235541,
0.06554457545280457,
0.01190351601690054,
0.053970545530319214,
0.03071771003305912,
0.03732047602534294,
-0.03564727306365967,
-0.006394069641828537,
-0.022980017587542534,
0.011952620930969715,
0.0786571055650711,
0.04456285014748573,
0.07406643033027649,
-0.03096233867108822,
0.005232551135122776,
-0.009082947857677937,
-0.003495462005957961,
0.00650978647172451,
-0.00565964775159955,
0.040754787623882294,
-0.011968892998993397,
-0.0296016838401556,
0.004687813576310873,
-0.04477153718471527,
0.011251844465732574,
0.06840332597494125,
-0.28466710448265076,
-0.0030579923186451197,
-0.012341992929577827,
0.01803227886557579,
-0.04312223196029663,
-0.011267627589404583,
0.09945916384458542,
-0.028460904955863953,
-0.023786915466189384,
0.020261570811271667,
-0.010888518765568733,
0.023868348449468613,
0.06254106760025024,
0.05318394675850868,
0.03213300183415413,
-0.023724889382719994,
0.04921691492199898,
-0.03767396882176399,
-0.016974616795778275,
-0.05826853588223457,
0.03607769310474396,
-0.008602523244917393,
0.15461014211177826,
0.012363498099148273,
-0.008897950872778893,
0.08572050929069519,
-0.008220798335969448,
0.047148603945970535,
-0.02096247300505638,
-0.0336257703602314,
-0.025576209649443626,
0.02346443571150303,
0.038432955741882324,
0.015718402341008186,
-0.017915701493620872,
0.017100945115089417,
-0.0104007413610816,
0.032613370567560196,
-0.04689688980579376,
0.014783904887735844,
-0.026076558977365494,
-0.027318134903907776,
-0.07683847099542618,
-0.048994097858667374,
0.10864031314849854,
-0.0664459839463234,
-0.02253284864127636,
-0.09327033907175064,
-0.02220243401825428,
0.012448148801922798,
-0.02136390097439289,
0.01187590416520834,
-0.020350422710180283,
-0.02987748757004738,
0.02944626286625862,
0.025739500299096107,
-0.013993254862725735,
0.014165815897285938,
0.040226224809885025,
-0.013476993888616562,
-0.05056673660874367,
0.006527537479996681,
0.03208203986287117,
0.08860877901315689,
0.0015429722843691707
] |
PMID:18471
|
Partial purification of 6-(D-erythro-1',2',3'-trihydroxypropyl)-7,8-dihydropterin triphosphate synthetase from chicken liver.
|
An enzyme that catalyzes the formation of 6-(D-erythro-1',2',3'-trihydroxypropyl)-7,8-dihydropterin triphosphate (D-erythrodihydroneopterin triphosphate) and formic acid from GTP has been purified about 3700-fold from homogenates of chicken liver. The molecular weight of the enzyme, D-erythrodihydroneopterin triphosphate synthetase (GTP cyclohydrolase), has been estimated to be 125,000 by gel filtration on Ultrogel AcA-34. The enzyme functions optimally between pH 8.0 and 9.2 and is considerably heat-stable. No cofactors or metal ions have been demonstrated to be required for activity; however, the reaction is strongly inhibited by Cu2+ and Hg2+. GTP is the most efficient substrate, with GDP being 1/17 as active and guanosine, GMP, and ATP being inactive. The Km for GTP has been found to be 14 micrometer. Although the overall reaction catalyzed by D-erythrodihydroneopterin triphosphate synthetase from chicken liver is identical with that from Escherichia coli GTP cyclohydrolase, immunological studies show no apparent homology between the two enzymes.
|
Partial purification of 6-(D-erythro-1',2',3'-trihydroxypropyl)-7,8-dihydropterin triphosphate synthetase from chicken liver. An enzyme that catalyzes the formation of 6-(D-erythro-1',2',3'-trihydroxypropyl)-7,8-dihydropterin triphosphate (D-erythrodihydroneopterin triphosphate) and formic acid from GTP has been purified about 3700-fold from homogenates of chicken liver. The molecular weight of the enzyme, D-erythrodihydroneopterin triphosphate synthetase (GTP cyclohydrolase), has been estimated to be 125,000 by gel filtration on Ultrogel AcA-34. The enzyme functions optimally between pH 8.0 and 9.2 and is considerably heat-stable. No cofactors or metal ions have been demonstrated to be required for activity; however, the reaction is strongly inhibited by Cu2+ and Hg2+. GTP is the most efficient substrate, with GDP being 1/17 as active and guanosine, GMP, and ATP being inactive. The Km for GTP has been found to be 14 micrometer. Although the overall reaction catalyzed by D-erythrodihydroneopterin triphosphate synthetase from chicken liver is identical with that from Escherichia coli GTP cyclohydrolase, immunological studies show no apparent homology between the two enzymes.
|
[
-0.09791889786720276,
-0.058932315558195114,
0.035364653915166855,
0.0041793822310864925,
0.02112305723130703,
0.00548426853492856,
-0.011097822338342667,
0.028884442523121834,
-0.06043955683708191,
0.016531240195035934,
0.010490518063306808,
-0.060366589576005936,
-0.049776725471019745,
-0.010567381046712399,
-0.041786205023527145,
-0.036839306354522705,
-0.01296217180788517,
0.0006152851274237037,
-0.01679975911974907,
0.023926356807351112,
0.023286152631044388,
-0.014564262703061104,
0.025683335959911346,
-0.06906795501708984,
0.058688949793577194,
0.042505115270614624,
0.005836429074406624,
0.029688760638237,
-0.0413966104388237,
-0.1668100655078888,
0.03327574580907822,
0.017367955297231674,
0.004977792967110872,
-0.0565185621380806,
-0.04791664704680443,
0.0331551767885685,
-0.0491730235517025,
-0.0428520143032074,
-0.03324452042579651,
-0.008193125948309898,
-0.0009104288765229285,
0.007398976478725672,
-0.0563647523522377,
-0.003331286134198308,
0.013414505869150162,
0.036694757640361786,
-0.040698327124118805,
0.0346112884581089,
0.02266986295580864,
-0.0043966928496956825,
0.014739308506250381,
0.025693830102682114,
-0.04834316670894623,
0.0069649540819227695,
0.011038348078727722,
-0.022064311429858208,
0.10059183090925217,
0.013803903013467789,
-0.027033941820263863,
-0.035690389573574066,
-0.008076295256614685,
0.01631188951432705,
-0.14219415187835693,
0.018258174881339073,
0.04548249393701553,
-0.020437002182006836,
-0.04565562307834625,
-0.05977126955986023,
0.017698876559734344,
0.033417150378227234,
-0.05830136314034462,
0.0027189424727112055,
-0.0350596122443676,
0.029620515182614326,
-0.04558282345533371,
0.0027808528393507004,
-0.017124535515904427,
0.005567962769418955,
0.023606346920132637,
0.045754026621580124,
0.03291242942214012,
0.040620021522045135,
0.002445349469780922,
0.0041842530481517315,
0.004423307254910469,
0.012307099997997284,
-0.03476935252547264,
-0.004963582381606102,
-0.003779767081141472,
0.037451501935720444,
0.0035101575776934624,
-0.08202310651540756,
0.03295345604419708,
-0.03482135012745857,
-0.106215700507164,
0.10191651433706284,
0.06025389954447746,
0.006800431292504072,
-0.017862951382994652,
0.3868255317211151,
-0.033539414405822754,
-0.008471690118312836,
-0.04611647129058838,
-0.005061677191406488,
-0.010523604229092598,
-0.05783893167972565,
-0.03667459264397621,
-0.006733968388289213,
-0.011718351393938065,
0.04853212833404541,
0.03599163144826889,
0.000014392787306860555,
0.03975823521614075,
-0.022472666576504707,
-0.008699855767190456,
-0.03583558276295662,
-0.039011187851428986,
-0.007950504310429096,
-0.028329819440841675,
0.07489977031946182,
0.00409889779984951,
-0.030324792489409447,
0.02599564753472805,
0.03225075826048851,
0.04234982281923294,
0.006662249565124512,
-0.06580725312232971,
0.08348874002695084,
0.07325316965579987,
0.012767062522470951,
0.024980641901493073,
0.02779470756649971,
-0.0059847766533494,
0.01181467529386282,
0.01293280627578497,
-0.006627272814512253,
0.10458818078041077,
-0.04676584154367447,
0.012493937276303768,
-0.04312537610530853,
-0.04599757120013237,
0.0024106036871671677,
-0.09367714822292328,
-0.025778047740459442,
-0.019257226958870888,
0.07551616430282593,
0.00038658405537717044,
0.013237178325653076,
-0.02042888104915619,
0.00617177551612258,
0.006708513014018536,
0.046094391494989395,
0.023324331268668175,
0.02642393484711647,
-0.03760089352726936,
0.0476781390607357,
0.015427468344569206,
-0.03250499814748764,
-0.008410959504544735,
-0.021087460219860077,
-0.08825081586837769,
-0.015635795891284943,
-0.026957888156175613,
0.06711611151695251,
0.022700250148773193,
-0.05059324949979782,
-0.05113779753446579,
0.01958429254591465,
-0.014738298952579498,
-0.003022406715899706,
0.03079235926270485,
0.07782842963933945,
-0.008419104851782322,
-0.03169739991426468,
-0.04782725125551224,
-0.03131327033042908,
0.0020902547985315323,
0.03020157292485237,
0.07195881009101868,
-0.00414550444111228,
0.0647151842713356,
-0.029151832684874535,
-0.05791362375020981,
-0.004976358730345964,
0.001699887216091156,
-0.04410674050450325,
-0.030634157359600067,
0.024814637377858162,
-0.023030955344438553,
0.02701994590461254,
-0.028478747233748436,
0.05148586630821228,
-0.046372704207897186,
-0.05207563191652298,
-0.05091751366853714,
-0.08988634496927261,
0.002759237540885806,
0.048988983035087585,
0.06086989492177963,
0.003498799866065383,
0.08660802990198135,
0.02597028575837612,
0.04753131419420242,
0.05782955139875412,
-0.048380982130765915,
0.005384126678109169,
0.0028576275799423456,
0.013462196104228497,
-0.03551976755261421,
0.030053207650780678,
0.02428337372839451,
0.02344256266951561,
-0.022873753681778908,
0.04939304292201996,
-0.07622139155864716,
0.07415207475423813,
-0.05341220274567604,
0.04481267184019089,
0.018390219658613205,
0.00775207718834281,
0.0492396280169487,
-0.09951779246330261,
-0.03367665410041809,
-0.2884676158428192,
-0.0790146067738533,
-0.023202087730169296,
-0.0262746699154377,
0.05170289799571037,
0.01989910565316677,
0.019603237509727478,
-0.03774578869342804,
0.051295869052410126,
0.0034746869932860136,
0.06418348848819733,
0.0739767998456955,
-0.017457544803619385,
-0.06125427410006523,
-0.060815341770648956,
0.03562567010521889,
0.04989035427570343,
-0.01941337063908577,
-0.008913787081837654,
-0.032245784997940063,
-0.004705705679953098,
0.022789165377616882,
0.012692158110439777,
0.033405106514692307,
0.08974853157997131,
-0.03490374982357025,
0.10051319748163223,
0.05122122913599014,
0.0396253801882267,
0.016514064744114876,
0.0037906267680227757,
0.07944774627685547,
0.022187156602740288,
-0.0674390196800232,
-0.021942483261227608,
-0.0229071993380785,
-0.0018935431726276875,
-0.08538288623094559,
0.040093567222356796,
-0.007236112374812365,
0.0248726736754179,
0.023849770426750183,
-0.018243582919239998,
-0.06403549760580063,
-0.04206671938300133,
-0.02608659118413925,
-0.018684986978769302,
0.03754013776779175,
-0.022534741088747978,
0.0003180240746587515,
0.03408607468008995,
0.04214969277381897,
0.05807055905461311,
-0.034044116735458374,
-0.03590293228626251,
0.007255998905748129,
0.016477931290864944,
0.021993795409798622,
-0.041855089366436005,
0.024631362408399582,
0.01839655451476574,
-0.015872448682785034,
-0.04266633838415146,
0.06174900010228157,
0.005204407963901758,
-0.03655186668038368,
0.04622052609920502,
-0.04558131843805313,
0.06589438021183014,
0.02546076476573944,
0.011757967062294483,
0.067459836602211,
-0.016935478895902634,
0.09243471175432205,
0.022731147706508636,
-0.04001098871231079,
-0.06729238480329514,
-0.03319157660007477,
-0.042375531047582626,
0.013008843176066875,
0.023241836577653885,
-0.03195565566420555,
0.05159434303641319,
0.010217537172138691,
0.01785758137702942,
0.012625577859580517,
-0.0019275650847703218,
0.06862732023000717,
0.06469526141881943,
-0.0331629179418087,
0.006022247951477766,
0.003890621941536665,
-0.05868557468056679,
-0.014748360961675644,
0.07839461416006088,
0.009771826677024364,
-0.28662052750587463,
0.02067531831562519,
-0.013964151963591576,
0.0022814006078988314,
-0.06480297446250916,
0.005732582416385412,
0.043536871671676636,
-0.005301868077367544,
0.011277787387371063,
0.005425226874649525,
0.039467327296733856,
0.0015232714358717203,
0.03710942342877388,
0.06871596723794937,
-0.025700710713863373,
-0.011566761881113052,
0.06896932423114777,
-0.013558504171669483,
-0.02748412825167179,
-0.012944185175001621,
0.003529253648594022,
0.006240139715373516,
0.10474754124879837,
-0.019978895783424377,
-0.01689273864030838,
0.030189868062734604,
0.05605397745966911,
0.003326904494315386,
-0.05853530392050743,
0.014141225256025791,
-0.048227254301309586,
0.01735776662826538,
0.07866861671209335,
-0.0866108164191246,
0.04712001606822014,
0.05076709762215614,
0.03825610876083374,
0.013790509663522243,
-0.04032186046242714,
-0.0016675773076713085,
-0.01750967837870121,
0.0023702781181782484,
-0.039594296365976334,
0.002776957815513015,
0.07525882869958878,
-0.08000252395868301,
-0.029706887900829315,
-0.03318151459097862,
0.002027805894613266,
-0.01984277181327343,
-0.01303743477910757,
0.04688452556729317,
0.019048597663640976,
-0.010993934236466885,
-0.00980597548186779,
-0.02454114705324173,
-0.012288277968764305,
0.06091691553592682,
-0.019470348954200745,
-0.03171468526124954,
-0.015765421092510223,
-0.00831652246415615,
0.034135960042476654,
0.10666850209236145,
0.048654377460479736
] |
PMID:18475
|
Selective de novo synthesis of tyrosine hydroxylase in organ cultures of rat superior cervical ganglia after in vivo administration of nerve growth factor.
|
Tyrosine hydroxylase is synthesized de novo in rat superior cervical ganglia in organ culture. The differential rate of synthesis is not increased significantly by the addition of nerve growth factor to the culture. Prior administration of nerve growth factor in vivo, however, leads to an augmented synthesis of tyrosine hydroxylase in ganglia subsequently cultured in vitro. The differential rate of tyrosine hydroxylase synthesis was increased by a factor of between 3 and 4. Increases in the differential rate of synthesis were detected within 6 h; the rate reached a maximum 24 to 36 h after a single injection of nerve growth factor. Administration of actinomycin D or of nerve growth factor antibody in vivo prevented the nerve growth factor-induced increase in the differential rate of tyrosine hydroxylase synthesis in vitro. However, the increase in the synthetic rate of tyrosine hydroxylase was not prevented by the addition of actinomycin D to the culture.
|
Selective de novo synthesis of tyrosine hydroxylase in organ cultures of rat superior cervical ganglia after in vivo administration of nerve growth factor. Tyrosine hydroxylase is synthesized de novo in rat superior cervical ganglia in organ culture. The differential rate of synthesis is not increased significantly by the addition of nerve growth factor to the culture. Prior administration of nerve growth factor in vivo, however, leads to an augmented synthesis of tyrosine hydroxylase in ganglia subsequently cultured in vitro. The differential rate of tyrosine hydroxylase synthesis was increased by a factor of between 3 and 4. Increases in the differential rate of synthesis were detected within 6 h; the rate reached a maximum 24 to 36 h after a single injection of nerve growth factor. Administration of actinomycin D or of nerve growth factor antibody in vivo prevented the nerve growth factor-induced increase in the differential rate of tyrosine hydroxylase synthesis in vitro. However, the increase in the synthetic rate of tyrosine hydroxylase was not prevented by the addition of actinomycin D to the culture.
|
[
-0.04477214813232422,
-0.006711766589432955,
-0.04995667561888695,
-0.020349089056253433,
-0.040764208883047104,
0.032973598688840866,
0.021553335711359978,
0.019541993737220764,
-0.034762654453516006,
0.01609695516526699,
0.06815507262945175,
-0.07274167984724045,
0.007631608285009861,
0.015311505645513535,
-0.0339728519320488,
-0.040811534970998764,
-0.029518546536564827,
-0.0231583584100008,
-0.059111520648002625,
-0.04480859264731407,
-0.0320422388613224,
0.0007802928448654711,
0.03237363323569298,
-0.06946362555027008,
0.07937348634004593,
0.01779586263000965,
-0.03160803020000458,
0.047518838196992874,
-0.014031299389898777,
-0.18568155169487,
0.0051477630622684956,
0.011626891791820526,
0.017391173169016838,
-0.060689929872751236,
0.008283909410238266,
-0.017072508111596107,
-0.03330102935433388,
-0.02749386802315712,
-0.003948806785047054,
0.02208097279071808,
0.0076668355613946915,
0.046457353979349136,
0.00007425182411679998,
0.0292464941740036,
0.014117063023149967,
0.0011671502143144608,
-0.04641197621822357,
0.012891224585473537,
0.0655183494091034,
-0.0022070256527513266,
0.00517989881336689,
-0.04609459638595581,
0.019491279497742653,
0.06785165518522263,
0.033442310988903046,
-0.0009322126861661673,
0.02400779537856579,
0.04006720334291458,
0.0023223687894642353,
0.025327272713184357,
0.02281292900443077,
0.06310445070266724,
-0.1533605456352234,
0.032431889325380325,
0.014691732823848724,
-0.018093932420015335,
0.0015966587234288454,
-0.06094380095601082,
0.05510303005576134,
0.04405966028571129,
-0.08203061670064926,
0.03665928170084953,
0.02418820559978485,
0.009200159460306168,
0.010758994147181511,
0.02034759148955345,
-0.06805842369794846,
0.024445505812764168,
0.06594350934028625,
0.023310400545597076,
0.028710737824440002,
0.0005719210603274405,
-0.007579141296446323,
-0.028373373672366142,
-0.008684604428708553,
-0.008929471485316753,
0.027512559667229652,
0.004945680499076843,
-0.0018886129837483168,
0.007220686879009008,
-0.022757545113563538,
-0.0788772702217102,
-0.006221991032361984,
0.02072945609688759,
-0.04605035111308098,
0.029543442651629448,
0.0009672027081251144,
0.02659107930958271,
-0.07339981943368912,
0.3560391664505005,
-0.02459186129271984,
-0.051610011607408524,
-0.059996478259563446,
-0.009986692108213902,
-0.1041555404663086,
-0.020759250968694687,
-0.012109851464629173,
0.027723565697669983,
-0.029583213850855827,
0.07380198687314987,
0.053468793630599976,
0.025654984638094902,
0.011798393912613392,
-0.052494924515485764,
-0.07833229750394821,
-0.018815578892827034,
-0.010322628542780876,
-0.002962025348097086,
-0.027120506390929222,
0.034461650997400284,
0.011467566713690758,
0.04403083398938179,
0.02186737209558487,
-0.0818762481212616,
0.004315412137657404,
-0.035537708550691605,
-0.07653909176588058,
0.09644614160060883,
0.06355109065771103,
-0.003876264439895749,
0.0004900273052044213,
0.03923259675502777,
-0.026494843885302544,
0.02192874439060688,
0.06314802914857864,
0.004745036363601685,
0.011329145170748234,
-0.03118336945772171,
-0.04400261864066124,
-0.060313235968351364,
0.026543764397501945,
-0.005680912174284458,
0.002422739053145051,
-0.026923883706331253,
-0.057464249432086945,
0.1255834847688675,
0.021094119176268578,
-0.04761258512735367,
-0.025799212977290154,
0.006901276297867298,
0.0376557894051075,
0.0769820511341095,
-0.04998095706105232,
0.026679586619138718,
-0.007325278595089912,
0.016352375969290733,
-0.043736040592193604,
0.0010025235824286938,
-0.011047464795410633,
-0.03596411272883415,
-0.03638926520943642,
0.02951875329017639,
-0.010668784379959106,
0.05356144160032272,
-0.002143378369510174,
-0.023751916363835335,
-0.08452285826206207,
0.07571184635162354,
0.02373863197863102,
0.0377049595117569,
-0.00666800094768405,
-0.021359287202358246,
-0.00853333342820406,
-0.012882769107818604,
-0.06099751219153404,
-0.07459261268377304,
0.0008036874933168292,
0.008935493417084217,
0.005533342715352774,
0.03802283853292465,
0.062117692083120346,
-0.041278231889009476,
-0.0337972491979599,
0.017262140288949013,
-0.005949036683887243,
-0.04164029657840729,
-0.0016332296654582024,
-0.021969955414533615,
0.027691682800650597,
0.03093944862484932,
-0.007834021002054214,
0.06558211892843246,
-0.045830391347408295,
-0.015210787765681744,
0.005021578632295132,
-0.03715597838163376,
-0.04714413359761238,
0.10431482642889023,
0.010815569199621677,
0.011243588291108608,
0.06840715557336807,
0.001042347401380539,
-0.033824123442173004,
0.07014136016368866,
0.014644457958638668,
0.04634586721658707,
0.008124935440719128,
-0.009689409285783768,
0.06275642663240433,
0.035947155207395554,
-0.0838494524359703,
-0.01715835928916931,
0.04549829661846161,
0.020750924944877625,
-0.04792996123433113,
0.07249316573143005,
-0.004118851386010647,
-0.001350800390355289,
0.014426671899855137,
0.0035436851903796196,
0.08489520102739334,
-0.059524886310100555,
-0.012614908628165722,
-0.2777995765209198,
-0.02373451367020607,
-0.017081743106245995,
-0.04449338838458061,
0.05159049853682518,
-0.03946676850318909,
0.057283028960227966,
-0.025539087131619453,
0.0338665246963501,
-0.029206743463873863,
0.06428276747465134,
0.11002768576145172,
0.015615709125995636,
-0.062038544565439224,
0.01878872886300087,
0.07584528625011444,
0.026378287002444267,
-0.01694849506020546,
0.05324575677514076,
-0.024246931076049805,
0.014532776549458504,
0.02942536026239395,
0.048731617629528046,
-0.0015924237668514252,
0.027140775695443153,
-0.05260840430855751,
0.07751572877168655,
0.012463228777050972,
0.018040861934423447,
-0.013121544383466244,
0.08328568935394287,
0.08456242829561234,
-0.020741702988743782,
-0.11921728402376175,
-0.011585020460188389,
-0.05994545295834541,
0.04783128574490547,
0.012585279531776905,
-0.032684896141290665,
-0.011419836431741714,
0.011681171134114265,
-0.026725824922323227,
-0.031121909618377686,
-0.06607312709093094,
-0.04025741666555405,
-0.03850049525499344,
0.05588610842823982,
-0.0153932711109519,
-0.026410533115267754,
-0.018768802285194397,
0.06855650991201401,
-0.04193994402885437,
0.006844833958894014,
-0.012639205902814865,
-0.04600278660655022,
-0.05491418391466141,
-0.05701345205307007,
-0.05151840299367905,
-0.08763280510902405,
-0.02223842591047287,
0.02577976882457733,
0.014532730914652348,
0.004467274062335491,
-0.0705127865076065,
-0.011347891762852669,
0.053769223392009735,
0.026263220235705376,
-0.00941479206085205,
0.014356774277985096,
0.03450062498450279,
0.01869206689298153,
0.05059906095266342,
0.04157152771949768,
0.05680416151881218,
0.07859110087156296,
-0.026166805997490883,
-0.04922019690275192,
-0.024205967783927917,
-0.09647255390882492,
0.012137324549257755,
-0.05002989619970322,
-0.06042321398854256,
0.03285688906908035,
0.024226898327469826,
-0.04601162672042847,
0.013385615311563015,
0.03325615078210831,
-0.003162183566018939,
0.012836172245442867,
0.011771781370043755,
0.03769810125231743,
0.023807542398571968,
-0.012427963316440582,
-0.010784049518406391,
0.02540108561515808,
-0.027434829622507095,
-0.260194331407547,
-0.00422399677336216,
0.059290315955877304,
-0.043500181287527084,
-0.020207390189170837,
0.05640168860554695,
0.010185737162828445,
0.001076237764209509,
-0.06196489930152893,
0.0586225688457489,
0.03722098469734192,
-0.004970918875187635,
0.08438847959041595,
0.09139639884233475,
0.02844391018152237,
-0.006477248854935169,
0.06021556258201599,
0.017852459102869034,
0.05950082093477249,
-0.07189024984836578,
0.013580003753304482,
-0.06678050011396408,
0.10065113008022308,
0.017450574785470963,
0.005063069052994251,
0.033386729657649994,
0.0255050640553236,
0.031775351613759995,
0.02195504494011402,
0.02243082784116268,
-0.028752220794558525,
-0.015762973576784134,
0.05729224905371666,
0.0008924953290261328,
0.04088769480586052,
0.037167057394981384,
-0.031919024884700775,
-0.01061253622174263,
-0.011724029667675495,
0.0332118384540081,
-0.056685056537389755,
0.021263640373945236,
-0.06413359940052032,
0.03425150364637375,
0.06894964724779129,
-0.06376583874225616,
0.009031551890075207,
-0.06643877923488617,
-0.03384265676140785,
-0.01776200719177723,
-0.018567077815532684,
0.020069066435098648,
0.02286086045205593,
0.045503050088882446,
0.03983740136027336,
-0.0032602609135210514,
0.020586350932717323,
-0.008871050551533699,
0.015024774707853794,
0.03509702533483505,
-0.030397877097129822,
-0.06762741506099701,
-0.017593299970030785,
0.10132256895303726,
-0.017432156950235367
] |
PMID:18477
|
Solubilization and partial purification of heme oxygenase from rat liver.
|
Hepatic microsomal heme oxygenase was solubilized, partially purified, and characterized from Co2+-treated rats. The enzyme on sodium dodecyl sulfate-polyacrylamide gel electrophoresis exhibited a minimum molecular weight of greater than or equal to 68,000. The solubilized enzyme was totally devoid of contamination with cytochrome P-450 or b5. The requirement for reduced pyridine nucleotides was absolute, and ascorbate could not support heme oxidative activity. However, both TPNH and DPNH could serve as electron donors, with TPNH being more effective. The presence of an appropriate flavoprotein reductase was essential for heme oxidation. The enzyme had an apparent Km of 40 micrometer, a pH optimum of 7.5, and lost substantial activity upon freezing and thawing. Methemoglobin was 30% as effective a substrate for the enzyme as was heme. Free porphyrins could not serve as substrates for the enzyme. The activity of the enzyme was inhibited by HgCl2, p-chloromercuribenzoate, iodoacetamide, mercaptoethanol, and dithiothrietol indicating that free -SH group(s) is necessary for enzyme activity.
|
Solubilization and partial purification of heme oxygenase from rat liver. Hepatic microsomal heme oxygenase was solubilized, partially purified, and characterized from Co2+-treated rats. The enzyme on sodium dodecyl sulfate-polyacrylamide gel electrophoresis exhibited a minimum molecular weight of greater than or equal to 68,000. The solubilized enzyme was totally devoid of contamination with cytochrome P-450 or b5. The requirement for reduced pyridine nucleotides was absolute, and ascorbate could not support heme oxidative activity. However, both TPNH and DPNH could serve as electron donors, with TPNH being more effective. The presence of an appropriate flavoprotein reductase was essential for heme oxidation. The enzyme had an apparent Km of 40 micrometer, a pH optimum of 7.5, and lost substantial activity upon freezing and thawing. Methemoglobin was 30% as effective a substrate for the enzyme as was heme. Free porphyrins could not serve as substrates for the enzyme. The activity of the enzyme was inhibited by HgCl2, p-chloromercuribenzoate, iodoacetamide, mercaptoethanol, and dithiothrietol indicating that free -SH group(s) is necessary for enzyme activity.
|
[
-0.07873399555683136,
0.04358072578907013,
-0.05969411879777908,
0.02080877125263214,
0.031186530366539955,
-0.05262669548392296,
0.0170799121260643,
0.011587580665946007,
-0.04674381762742996,
-0.013330274261534214,
-0.011935085989534855,
-0.06282460689544678,
-0.004402076359838247,
0.0478716678917408,
-0.05685620382428169,
0.009961646050214767,
0.04561368003487587,
-0.002979121170938015,
-0.08678726106882095,
0.026609329506754875,
-0.05351795256137848,
0.035141874104738235,
0.06746417284011841,
-0.0011317523894831538,
-0.004297404550015926,
0.012524770572781563,
0.009932024404406548,
-0.05974223464727402,
-0.020145690068602562,
-0.19484642148017883,
-0.004709166008979082,
-0.019470244646072388,
0.05694679170846939,
-0.017611846327781677,
0.03558117523789406,
0.011570964939892292,
-0.0335315465927124,
-0.046027060598134995,
-0.04913509264588356,
0.05370189994573593,
0.07982107996940613,
0.021728703752160072,
-0.005993704777210951,
0.042798347771167755,
0.01715855300426483,
0.006715844850987196,
0.02167273499071598,
0.0000801573260105215,
0.04045318812131882,
-0.010339141823351383,
0.09856519848108292,
0.026512864977121353,
-0.009134172461926937,
-0.024673091247677803,
0.03903023526072502,
0.018931174650788307,
0.0531531423330307,
0.051072292029857635,
-0.03137802332639694,
0.017393356189131737,
-0.01996159739792347,
0.020828133448958397,
-0.1095101460814476,
0.0559774674475193,
0.07249864190816879,
-0.010635237209498882,
-0.0016865914221853018,
-0.09754666686058044,
0.08455410599708557,
0.016586659476161003,
0.005603992845863104,
0.06595543771982193,
-0.030441099777817726,
0.024308307096362114,
-0.027565225958824158,
-0.004807846620678902,
0.004691477864980698,
-0.028394188731908798,
0.025675754994153976,
0.0210551917552948,
0.006267778109759092,
0.029425660148262978,
0.06017297878861427,
-0.005104800220578909,
0.014261600561439991,
0.0421844944357872,
-0.013139666989445686,
-0.006956849712878466,
-0.02454563044011593,
0.0068438611924648285,
-0.029632188379764557,
-0.026267610490322113,
-0.021402478218078613,
-0.03444189205765724,
-0.08325395733118057,
0.03725992143154144,
0.057971756905317307,
0.05085139721632004,
-0.044125668704509735,
0.3877561092376709,
0.006491649895906448,
-0.0017204274190589786,
-0.06532884389162064,
-0.0628637745976448,
-0.016075417399406433,
-0.02118947170674801,
-0.005139015149325132,
0.0059282612055540085,
0.008321241475641727,
0.05210346728563309,
0.02795497700572014,
-0.014467407949268818,
0.022437477484345436,
-0.04809289425611496,
-0.028612840920686722,
0.019431211054325104,
-0.015162463299930096,
-0.002402824815362692,
-0.010337651707231998,
0.06925215572118759,
-0.019030913710594177,
-0.020477421581745148,
0.026284947991371155,
0.04219164699316025,
-0.020942160859704018,
0.00031117486651055515,
0.01229384820908308,
0.07713713496923447,
0.06912076473236084,
0.03655707836151123,
0.033100832253694534,
0.03414936736226082,
0.003575732698664069,
-0.00794092658907175,
-0.06056125834584236,
-0.046590905636548996,
0.055754564702510834,
-0.02401684783399105,
0.007555932737886906,
0.008328753523528576,
-0.052904266864061356,
-0.0454421266913414,
-0.06969590485095978,
-0.06243833899497986,
-0.07967722415924072,
0.044047851115465164,
0.01665458455681801,
0.03784927353262901,
0.020938389003276825,
-0.07776476442813873,
-0.007265962660312653,
0.04205535352230072,
-0.03796028345823288,
0.04963672533631325,
0.005958715453743935,
0.01704968512058258,
-0.00969142559915781,
-0.04539651796221733,
0.04435475170612335,
-0.018194084987044334,
-0.05397284775972366,
-0.010122943669557571,
-0.008995568379759789,
0.07452436536550522,
-0.02099955454468727,
0.01231133658438921,
-0.06786856800317764,
0.06766311079263687,
0.05939025804400444,
-0.014373461715877056,
0.026749033480882645,
-0.031081821769475937,
-0.0028455746360123158,
-0.03926212340593338,
-0.035576026886701584,
0.03655993565917015,
0.05130156874656677,
0.037240512669086456,
0.033843930810689926,
0.0102933868765831,
0.049839913845062256,
-0.019799262285232544,
-0.03465079888701439,
0.008613639511168003,
0.03225737810134888,
0.010674301534891129,
-0.00172800465952605,
-0.004787503741681576,
-0.022670654579997063,
0.06559577584266663,
-0.07208384573459625,
-0.0027059668209403753,
-0.05789000540971756,
-0.013556942343711853,
0.009309516288340092,
-0.040435947477817535,
-0.06513877213001251,
0.012491499073803425,
0.03907141834497452,
-0.0172269344329834,
0.1196378767490387,
0.0007442751666530967,
0.01749538816511631,
0.0343325138092041,
-0.02525440976023674,
0.047582708299160004,
0.041200313717126846,
0.015727456659078598,
0.030428197234869003,
0.02243499457836151,
-0.03331589698791504,
0.0007332857348956168,
-0.07536888867616653,
0.034874461591243744,
-0.05233726650476456,
0.03349962458014488,
0.004468435887247324,
-0.021072305738925934,
0.06787554174661636,
0.007416402921080589,
0.016642741858959198,
-0.12935759127140045,
-0.030680980533361435,
-0.3004727065563202,
-0.0457540862262249,
0.015203871764242649,
-0.04685083031654358,
0.016911614686250687,
-0.03777633234858513,
-0.02165851928293705,
-0.044846635311841965,
-0.016261018812656403,
0.022742364555597305,
0.06584496796131134,
0.029158713296055794,
-0.01857667602598667,
-0.01935693807899952,
0.0027042070869356394,
-0.004784184042364359,
0.03365739807486534,
-0.06309795379638672,
0.046833980828523636,
0.024611014872789383,
0.07880043983459473,
-0.03787192702293396,
0.011901951394975185,
0.030845414847135544,
0.025952812284231186,
0.04345056787133217,
0.09672026336193085,
-0.05482901260256767,
0.012600467540323734,
-0.00788173358887434,
0.04898610711097717,
0.03325246647000313,
-0.06189892441034317,
-0.09049776941537857,
-0.00365494005382061,
-0.05603132024407387,
-0.054388321936130524,
-0.09754756838083267,
0.048586294054985046,
0.024891620501875877,
-0.031559646129608154,
0.001656487351283431,
0.0008204263285733759,
-0.07155673950910568,
-0.0288541279733181,
-0.048991650342941284,
-0.013204519636929035,
0.002472601132467389,
0.003063581185415387,
-0.01602271944284439,
0.04410295560956001,
-0.03514173626899719,
0.006623976398259401,
-0.02796608954668045,
0.01608993113040924,
0.010750854387879372,
-0.01866043545305729,
0.04900133237242699,
-0.08069955557584763,
-0.009085613302886486,
-0.03342290595173836,
-0.043149471282958984,
-0.05895894020795822,
0.03873353824019432,
0.0068286401219666,
0.03427601978182793,
0.029748378321528435,
-0.046152640134096146,
0.07775924354791641,
0.011479773558676243,
0.029472483322024345,
0.06695973128080368,
-0.05567564442753792,
0.01911027915775776,
-0.012476233765482903,
0.041880447417497635,
-0.005512528587132692,
-0.026740243658423424,
-0.013744363561272621,
0.05503057688474655,
-0.00836584996432066,
0.015080967918038368,
0.06343554705381393,
0.046621181070804596,
-0.02742985635995865,
0.0672924667596817,
0.014129746705293655,
-0.0436132587492466,
0.030004864558577538,
0.018295904621481895,
0.03554448485374451,
-0.025088312104344368,
-0.04075220972299576,
-0.03092775121331215,
0.04916011169552803,
0.010951647534966469,
-0.23691976070404053,
-0.0009890575893223286,
0.030595727264881134,
0.04336167126893997,
-0.004451857879757881,
0.02134072035551071,
0.05086255073547363,
-0.07378627359867096,
-0.005270745605230331,
0.018226424232125282,
0.010820391587913036,
0.0320046991109848,
0.03145597502589226,
0.06212623417377472,
0.05489722639322281,
-0.034786779433488846,
0.04222598299384117,
-0.03381572663784027,
-0.0468229278922081,
-0.03585314750671387,
-0.008245027624070644,
-0.05246415361762047,
0.12013620883226395,
-0.022239860147237778,
-0.04243074730038643,
0.04182233288884163,
-0.008241819217801094,
0.017155762761831284,
-0.06744296848773956,
0.02514038234949112,
0.029899539425969124,
-0.013221911154687405,
0.12555643916130066,
-0.05536278337240219,
0.025094375014305115,
0.05247436463832855,
-0.02943464182317257,
0.010247674770653248,
0.004151881672441959,
0.02850801683962345,
-0.028418775647878647,
-0.005755344405770302,
-0.07042484730482101,
-0.04852099344134331,
0.03569713234901428,
-0.06577721238136292,
-0.03584931045770645,
-0.037010565400123596,
-0.02176586352288723,
-0.008710871450603008,
-0.038232073187828064,
-0.013778084889054298,
0.07073500007390976,
-0.004526046104729176,
0.008541492745280266,
-0.028645480051636696,
-0.028590340167284012,
0.02574777603149414,
0.0039532664231956005,
-0.03204715624451637,
-0.02607424557209015,
-0.014748208224773407,
-0.01248195767402649,
0.12533938884735107,
0.05805154889822006
] |
PMID:18479
|
Biochemical and cytochemical evidence for ATPase activity in basal bodies isolated from oviduct.
|
Biochemical and cytochemical techniques were used to determine whether oviduct basal bodies have ATPase activity. All studies were carried out on basal bodies isolated and purified from the chicken oviduct. These preparations contained structurally intact basal bodies with basal feet, rootlet, and alar sheet accessory structures. Whereas the specific activity of the basal body ATPase in 2 mM Ca++ or 2 mM Mg++, 1 mM ATP, pH 8.0, averaged 0.04 mumol Pi/min per mg protein, higher concentrations of either cation inhibited the enzyme activity. Furthermore, the pH optimum for this reaction was pH 8.5. In comparison, the ATPase activity in cilia purified and measured under conditions identical to those for determining the basal body ATPase activity averaged 0.07 mumol Pi/min per mg protein. However, the activity increased at higher concentrations of divalent cation, and the pH optimum was pH 10.0. By cytochemical procedures for localizing ATPase activity, ATP-dependent reaction product in isolated basal bodies was found to be confined to: (a) the cross-striations of the rootlet; (b) the outer portion of the basal foot; (c) the alar sheets; and (d) the triplet microtubules. It is concluded that basal bodiesve an intrinsic ATPase activity that, by a variety of criteria, can be distinguished from the ATPase activity found in cilia.
|
Biochemical and cytochemical evidence for ATPase activity in basal bodies isolated from oviduct. Biochemical and cytochemical techniques were used to determine whether oviduct basal bodies have ATPase activity. All studies were carried out on basal bodies isolated and purified from the chicken oviduct. These preparations contained structurally intact basal bodies with basal feet, rootlet, and alar sheet accessory structures. Whereas the specific activity of the basal body ATPase in 2 mM Ca++ or 2 mM Mg++, 1 mM ATP, pH 8.0, averaged 0.04 mumol Pi/min per mg protein, higher concentrations of either cation inhibited the enzyme activity. Furthermore, the pH optimum for this reaction was pH 8.5. In comparison, the ATPase activity in cilia purified and measured under conditions identical to those for determining the basal body ATPase activity averaged 0.07 mumol Pi/min per mg protein. However, the activity increased at higher concentrations of divalent cation, and the pH optimum was pH 10.0. By cytochemical procedures for localizing ATPase activity, ATP-dependent reaction product in isolated basal bodies was found to be confined to: (a) the cross-striations of the rootlet; (b) the outer portion of the basal foot; (c) the alar sheets; and (d) the triplet microtubules. It is concluded that basal bodiesve an intrinsic ATPase activity that, by a variety of criteria, can be distinguished from the ATPase activity found in cilia.
|
[
-0.03460303694009781,
-0.049233488738536835,
0.03769504278898239,
0.0331360399723053,
0.02019658498466015,
-0.03410031646490097,
0.059885721653699875,
0.038416244089603424,
0.023583460599184036,
0.010321179404854774,
0.013149183243513107,
-0.09093677252531052,
-0.047111641615629196,
0.04874367266893387,
-0.03005763702094555,
-0.05064409598708153,
-0.03187013790011406,
0.023225948214530945,
0.00862171407788992,
-0.03326774016022682,
0.014635231345891953,
-0.07073581218719482,
-0.022037450224161148,
-0.007437414024025202,
0.01816956140100956,
0.027338704094290733,
0.006933792028576136,
-0.013660998083651066,
-0.019029779359698296,
-0.17292903363704681,
-0.0037813300732523203,
-0.053223997354507446,
-0.04361452907323837,
-0.000972153851762414,
0.030974192544817924,
0.014048909768462181,
-0.007081914693117142,
0.023528801277279854,
-0.0756528303027153,
0.06452872604131699,
0.01681174337863922,
0.023767033591866493,
-0.01122618280351162,
0.03619745001196861,
0.056448109447956085,
-0.01020412240177393,
-0.027456235140562057,
0.03313814848661423,
0.07837123423814774,
-0.006032646633684635,
0.01856059394776821,
0.012976530008018017,
0.0066114854998886585,
0.019943425431847572,
-0.016375374048948288,
-0.015080736950039864,
0.034116920083761215,
0.03473670780658722,
-0.026909073814749718,
-0.021548757329583168,
0.007244111970067024,
0.054814837872982025,
-0.10739810764789581,
0.033621810376644135,
0.03226699307560921,
-0.007307599298655987,
-0.007804462686181068,
0.010212425142526627,
0.025363195687532425,
0.06616628915071487,
-0.05947357043623924,
0.07144933193922043,
0.03439759835600853,
0.024716505780816078,
0.03284040465950966,
-0.05190787464380264,
0.008885202929377556,
0.009276456199586391,
0.013985533267259598,
-0.04113088548183441,
-0.046213019639253616,
0.0029783754143863916,
0.01455361396074295,
0.017429981380701065,
0.020765986293554306,
0.034160248935222626,
0.008700662292540073,
-0.06801721453666687,
0.013727047480642796,
0.032411228865385056,
-0.029929017648100853,
-0.05310305953025818,
-0.043531354516744614,
-0.03570076450705528,
-0.08925461769104004,
0.07556978613138199,
0.0373656339943409,
0.017931820824742317,
0.019721288233995438,
0.40841802954673767,
0.0005563226877711713,
0.027219312265515327,
-0.04051196575164795,
0.0015190960839390755,
-0.017807740718126297,
-0.0148693872615695,
-0.031452715396881104,
0.008424813859164715,
-0.012646003626286983,
0.10256225615739822,
0.027955515310168266,
0.0660487487912178,
-0.03774544596672058,
-0.02072301134467125,
0.005286982748657465,
-0.04143355041742325,
0.0009097506408579648,
0.006093650124967098,
-0.04441182315349579,
0.07508567720651627,
-0.046927835792303085,
-0.01814844086766243,
0.1031990647315979,
-0.004318145103752613,
0.06394898891448975,
-0.001303241471759975,
-0.02546343021094799,
0.05578717216849327,
0.0994303971529007,
0.026905618607997894,
0.05299976095557213,
0.04059134051203728,
-0.004078792408108711,
-0.013497631065547466,
0.034766439348459244,
-0.05274534970521927,
0.053134627640247345,
0.0012811056803911924,
0.028401436284184456,
0.014700219966471195,
-0.06569738686084747,
-0.09681850671768188,
-0.028465287759900093,
-0.050090767443180084,
-0.045125383883714676,
0.07473786175251007,
0.019214756786823273,
0.030144715681672096,
0.0024687328841537237,
0.01738179847598076,
0.02719845622777939,
0.06741046905517578,
-0.04491667449474335,
0.023566389456391335,
-0.004164265003055334,
-0.027085810899734497,
-0.029594192281365395,
0.03722555190324783,
0.024529172107577324,
-0.040102601051330566,
-0.07710030674934387,
-0.034742970019578934,
0.01620621234178543,
0.09449052065610886,
0.012204629369080067,
-0.01989348791539669,
-0.003530481830239296,
0.03323298692703247,
-0.009175555780529976,
0.004411434754729271,
0.023380866274237633,
-0.01650329865515232,
-0.03542231395840645,
-0.044620580971241,
-0.01726176217198372,
-0.01137764286249876,
0.0014419675571843982,
0.03553811088204384,
0.006800094153732061,
0.01853019930422306,
0.015499234199523926,
-0.026766231283545494,
-0.0630204826593399,
-0.005327319726347923,
0.012166161090135574,
0.016431612893939018,
-0.0687922015786171,
0.0006388550391420722,
0.01973242312669754,
0.031721338629722595,
-0.057151541113853455,
-0.03346492350101471,
-0.09769362211227417,
0.012155176140367985,
-0.08690941333770752,
-0.08452699333429337,
0.01566704735159874,
0.01307008694857359,
-0.013932371512055397,
0.01316045317798853,
0.09804284572601318,
0.01665477454662323,
-0.014251050539314747,
0.08108575642108917,
-0.006143379490822554,
0.03024442493915558,
-0.04360087588429451,
0.03610977903008461,
0.030821794643998146,
0.0297553613781929,
-0.02709944359958172,
-0.017416488379240036,
-0.03803694248199463,
0.023654548451304436,
-0.053678907454013824,
0.055785298347473145,
-0.0032593240030109882,
-0.011784541420638561,
0.04624751955270767,
-0.017608676105737686,
0.027143675833940506,
-0.07636073231697083,
-0.03635239601135254,
-0.2896367013454437,
-0.08912074565887451,
0.01655971258878708,
-0.015795279294252396,
0.04112641513347626,
-0.020892156288027763,
0.022393817082047462,
0.00795688945800066,
0.04932598024606705,
-0.10015060007572174,
0.02292296104133129,
0.019672421738505363,
-0.024540426209568977,
-0.044618986546993256,
-0.04246121644973755,
0.06288332492113113,
-0.03346800059080124,
-0.02769845724105835,
-0.005437628831714392,
-0.021196750923991203,
0.04521019756793976,
-0.008895079605281353,
0.032713137567043304,
-0.010417756624519825,
0.07992365211248398,
-0.0671287253499031,
0.0929105207324028,
0.03115752898156643,
0.01891372725367546,
0.016741177067160606,
-0.0011902470141649246,
0.042171865701675415,
0.014932983554899693,
-0.09169872105121613,
-0.005316226277500391,
-0.025009799748659134,
0.00658989604562521,
-0.03732948750257492,
0.017310885712504387,
-0.006143698003143072,
-0.039035614579916,
0.0021326409187167883,
-0.028628835454583168,
-0.03080085664987564,
-0.03937103599309921,
0.011073523201048374,
0.042593907564878464,
0.04192662611603737,
0.013386975042521954,
-0.03698017820715904,
0.0716695711016655,
-0.03629358857870102,
0.03166617453098297,
-0.01911833882331848,
-0.052950553596019745,
-0.004328269045799971,
-0.027340998873114586,
0.02817455306649208,
-0.07148472219705582,
0.01929299905896187,
-0.0418710932135582,
0.045141708105802536,
-0.03462487831711769,
0.031127864494919777,
0.02199067920446396,
0.02651967667043209,
-0.008912572637200356,
-0.05581973120570183,
0.041528914123773575,
-0.04866694286465645,
0.04938201233744621,
0.05823802947998047,
-0.026504790410399437,
-0.020226897671818733,
0.036799173802137375,
0.01645008660852909,
-0.009769600816071033,
0.00048679375322535634,
-0.030428579077124596,
-0.013553819619119167,
-0.03447628766298294,
-0.0824773982167244,
0.0487743616104126,
-0.015586824156343937,
-0.0410129614174366,
0.026139631867408752,
0.01800115965306759,
-0.07167135924100876,
0.009624234400689602,
-0.03527109697461128,
0.0661877766251564,
0.007615456357598305,
-0.052241481840610504,
-0.004423272330313921,
0.04912904277443886,
0.032120876014232635,
-0.2876942753791809,
-0.019564302638173103,
0.05247724428772926,
-0.002511001192033291,
-0.021887345239520073,
0.005090185906738043,
0.04212280735373497,
0.03287341818213463,
0.0003429068310651928,
0.05288170278072357,
0.06537366658449173,
0.012663797475397587,
0.05231006443500519,
0.08418355137109756,
-0.046767327934503555,
0.06293699890375137,
0.06301267445087433,
-0.024370061233639717,
0.010276262648403645,
-0.03375956788659096,
-0.00023832242004573345,
-0.06289708614349365,
0.14475716650485992,
0.006587570533156395,
0.022506030276417732,
0.027447303757071495,
0.016076726838946342,
-0.02622123248875141,
0.004929408896714449,
-0.036417488008737564,
-0.012813063338398933,
-0.03567013889551163,
0.050354018807411194,
-0.019516106694936752,
0.019223762676119804,
0.048766184598207474,
0.011772802099585533,
0.015570125542581081,
0.010027921758592129,
-0.03280310332775116,
0.01283779926598072,
-0.03117666393518448,
-0.05474046990275383,
-0.062167271971702576,
0.012746705673635006,
-0.052475057542324066,
-0.04788228124380112,
-0.025054197758436203,
0.047692134976387024,
-0.011765211820602417,
0.004891577642410994,
-0.038082871586084366,
0.0005013333866372705,
0.05968358740210533,
0.0017960728146135807,
-0.0021753243636339903,
-0.03589856997132301,
-0.028193341568112373,
0.005364215932786465,
0.05951107293367386,
0.01617399975657463,
-0.04611152037978172,
0.011838329024612904,
0.09739986062049866,
0.04271413013339043
] |
PMID:18480
|
The activity of the pneumococcal autolytic system and the fate of the bacterium during ingestion by rabbit polymorphonuclear leukocytes.
|
The extent to which autolytic microbial enzymes are involved in the fate of microorganisms ingested by phagocytes has not been determined. It is known, however, that activation of degradative enzymes occurs during certain microbicidal events. We examined the possible role of the pneumococcal autolytic enzyme (an N-acetylmuramyl-L-alanine amidase) in the loss of viability and degradation of pneumococci during phagocytosis by rabbit polymorphonuclear leukocytes. Three bacterial systems were compared: (a) wild type pneumococci with an active autolytic system; (b) wild type bacteria grown under conditions that block the endogenous autolytic activity and (c) a mutant strain defective in the major autolytic enzyme of this bacterium. No differences could be detected between the autolysis-positive and negative bacteria in the rate of killing and in the fate of macromolecular cell constituents during ingestion by rabbit peritoneal polymorphonuclear leukocytes.
|
The activity of the pneumococcal autolytic system and the fate of the bacterium during ingestion by rabbit polymorphonuclear leukocytes. The extent to which autolytic microbial enzymes are involved in the fate of microorganisms ingested by phagocytes has not been determined. It is known, however, that activation of degradative enzymes occurs during certain microbicidal events. We examined the possible role of the pneumococcal autolytic enzyme (an N-acetylmuramyl-L-alanine amidase) in the loss of viability and degradation of pneumococci during phagocytosis by rabbit polymorphonuclear leukocytes. Three bacterial systems were compared: (a) wild type pneumococci with an active autolytic system; (b) wild type bacteria grown under conditions that block the endogenous autolytic activity and (c) a mutant strain defective in the major autolytic enzyme of this bacterium. No differences could be detected between the autolysis-positive and negative bacteria in the rate of killing and in the fate of macromolecular cell constituents during ingestion by rabbit peritoneal polymorphonuclear leukocytes.
|
[
-0.060408495366573334,
-0.006625968962907791,
-0.048350740224123,
-0.008636361919343472,
-0.00650910334661603,
-0.007266212720423937,
0.03503812476992607,
0.04726305603981018,
-0.04107102006673813,
-0.0034375269897282124,
0.027302471920847893,
-0.0546584315598011,
0.0021531160455197096,
-0.01673523709177971,
-0.004775350913405418,
-0.07836073637008667,
-0.07290533930063248,
0.04093455895781517,
-0.09269461780786514,
-0.017487546429038048,
-0.00390886515378952,
-0.022813908755779266,
-0.013489073142409325,
-0.02187495492398739,
0.03830365464091301,
0.024919874966144562,
0.045279160141944885,
-0.03204949200153351,
-0.031892456114292145,
-0.19344906508922577,
-0.002489756792783737,
-0.041570164263248444,
0.029519163072109222,
-0.008293047547340393,
-0.046760931611061096,
0.009642419405281544,
0.0018868857296183705,
-0.061341140419244766,
0.020563412457704544,
0.05907754972577095,
0.056489914655685425,
0.04299706220626831,
-0.002030422678217292,
0.020075762644410133,
0.014096518978476524,
0.00297013227827847,
0.009467186406254768,
0.015230429358780384,
0.096466563642025,
-0.00602889247238636,
-0.013601375743746758,
0.022737616673111916,
-0.008884986862540245,
0.05763784050941467,
-0.03197120502591133,
-0.08728516846895218,
0.003660330083221197,
0.009868323802947998,
0.024565601721405983,
0.021294886246323586,
0.04140859842300415,
-0.0034103954676538706,
-0.1284322887659073,
0.0727672353386879,
0.0291702039539814,
-0.0027896245010197163,
0.008025196380913258,
-0.04719699174165726,
0.03606569766998291,
0.03500230610370636,
-0.06546249985694885,
0.03383398428559303,
-0.02603161707520485,
0.03528616577386856,
-0.019621826708316803,
-0.015559648163616657,
0.04087682068347931,
-0.02616182342171669,
0.06982120126485825,
0.06308860331773758,
0.031014051288366318,
-0.007213131058961153,
0.005524751264601946,
-0.0076047880575060844,
-0.06406679004430771,
0.023191703483462334,
0.004731286782771349,
-0.06660117954015732,
0.03413313999772072,
0.046700820326805115,
-0.002889540744945407,
-0.029446667060256004,
0.021859942004084587,
0.04032076522707939,
-0.04867684468626976,
0.04340767860412598,
0.054133977741003036,
0.014158925972878933,
0.01118698064237833,
0.4195556044578552,
-0.041240375488996506,
-0.03632261976599693,
-0.0705752968788147,
0.0040296330116689205,
0.055965814739465714,
-0.010266316123306751,
-0.027886373922228813,
-0.044479940086603165,
-0.0032550934702157974,
-0.01805836893618107,
0.03746229037642479,
0.0038300612941384315,
-0.02882501855492592,
-0.013419053517282009,
0.006929144728928804,
0.033368222415447235,
0.043566811829805374,
0.035330552607774734,
-0.038142308592796326,
0.029988739639520645,
-0.011008166708052158,
-0.07037468254566193,
-0.007987738586962223,
-0.013098390772938728,
0.02127201110124588,
0.012554862536489964,
0.00586755620315671,
0.09109808504581451,
0.0014161908766254783,
-0.018141675740480423,
0.04212561622262001,
0.023340698331594467,
-0.0025176669005304575,
0.011275597847998142,
-0.004592719487845898,
-0.03829959034919739,
0.022616147994995117,
-0.04664386436343193,
0.041720278561115265,
-0.01848353073000908,
-0.04015178233385086,
-0.03694057837128639,
-0.06084215268492699,
-0.1064266562461853,
-0.02728670835494995,
0.07821886241436005,
-0.022425826638936996,
0.03581976890563965,
-0.02408483624458313,
0.002666738349944353,
0.0407412089407444,
0.06977840512990952,
-0.01951085962355137,
0.019191298633813858,
0.01834188774228096,
0.057902734726667404,
-0.009690135717391968,
-0.05048050358891487,
0.031619369983673096,
-0.026349883526563644,
-0.12945148348808289,
-0.003534069051966071,
0.02307470329105854,
0.06287071108818054,
0.02268752083182335,
0.017264792695641518,
-0.019831473007798195,
-0.03204192966222763,
0.03585340082645416,
-0.0023456637281924486,
0.04874996468424797,
0.01657181791961193,
-0.06577368080615997,
-0.019892262294888496,
-0.03838401287794113,
0.098897285759449,
0.015861842781305313,
0.010604552924633026,
0.023377014324069023,
0.053509097546339035,
-0.02096586860716343,
-0.01240548212081194,
0.02980101853609085,
0.009363469667732716,
0.03190195932984352,
-0.014598878100514412,
-0.026083942502737045,
0.029979072511196136,
0.04492906108498573,
-0.01791297271847725,
-0.013906661421060562,
-0.020711885765194893,
-0.05299195274710655,
-0.010536221787333488,
0.02463146112859249,
-0.07003754377365112,
-0.023881424218416214,
-0.009234552271664143,
0.01811656914651394,
0.014451484195888042,
0.07835660874843597,
0.022911202162504196,
0.029867064207792282,
0.006288767326623201,
0.029286306351423264,
0.012549678795039654,
-0.008929728530347347,
0.04997309297323227,
-0.004576492123305798,
-0.07343826442956924,
-0.06839077174663544,
-0.0653202086687088,
-0.08528326451778412,
0.034065090119838715,
-0.04557366296648979,
0.0027839653193950653,
-0.02680690586566925,
-0.012296125292778015,
0.04395969212055206,
-0.0004017815226688981,
0.011492113582789898,
0.019391575828194618,
0.011654437519609928,
-0.3084356188774109,
-0.03262616693973541,
-0.011636235751211643,
-0.0458010770380497,
-0.011551517061889172,
-0.011197920888662338,
0.014850636944174767,
-0.03866476193070412,
0.016782773658633232,
-0.0664110854268074,
0.059580665081739426,
0.050425413995981216,
-0.008085555396974087,
-0.03343569114804268,
0.0766308382153511,
0.07867714762687683,
-0.018177704885601997,
-0.09148664027452469,
-0.031399764120578766,
0.01625099964439869,
0.042944759130477905,
-0.06866181641817093,
0.046972449868917465,
0.0063055832870304585,
0.0343182310461998,
0.03388061001896858,
0.11308982223272324,
-0.0020398003980517387,
0.02342640981078148,
-0.015080871060490608,
0.06526356190443039,
0.06208237633109093,
-0.035444483160972595,
-0.06668224930763245,
-0.07911334931850433,
-0.012906077317893505,
-0.003775868797674775,
-0.04239748790860176,
-0.010454515926539898,
0.015216516330838203,
-0.03030170500278473,
0.05168802663683891,
-0.006872459780424833,
-0.07377614825963974,
-0.03186570852994919,
-0.03721693158149719,
0.021272728219628334,
0.06210176274180412,
-0.025820180773735046,
-0.011335701681673527,
0.013119260780513287,
-0.011631662026047707,
-0.024015720933675766,
-0.008551868610084057,
-0.0199654009193182,
0.046049948781728745,
0.0068761310540139675,
-0.022696418687701225,
-0.06874677538871765,
0.011166808195412159,
-0.028069259598851204,
-0.02729492262005806,
0.012014505453407764,
-0.019885379821062088,
0.021354829892516136,
-0.002816658467054367,
0.003481570165604353,
-0.01891445182263851,
0.047267790883779526,
0.009503429755568504,
-0.01754124090075493,
0.127949059009552,
-0.04743388295173645,
0.008216765709221363,
0.026523640379309654,
0.03048573061823845,
0.022152004763484,
-0.010971366427838802,
-0.10283711552619934,
-0.013870016671717167,
0.05116596072912216,
-0.08249437063932419,
0.06449558585882187,
-0.02823309414088726,
0.05090206488966942,
0.011079329065978527,
-0.008901304565370083,
0.020743172615766525,
-0.005368062760680914,
-0.029892386868596077,
-0.02442925050854683,
0.010179201140999794,
-0.07000835984945297,
-0.03578519821166992,
0.02764475718140602,
0.0332673080265522,
-0.2567813992500305,
0.01518422830849886,
0.027280202135443687,
0.026980474591255188,
-0.0035743985790759325,
0.0374995656311512,
0.01080232672393322,
-0.049914803355932236,
0.02574102021753788,
0.052249714732170105,
0.05852143466472626,
0.00553903728723526,
0.0379985012114048,
0.00013617950025945902,
-0.0015715567860752344,
0.048221196979284286,
0.03322523459792137,
0.020257430151104927,
0.034642525017261505,
0.01435522548854351,
-0.015415760688483715,
-0.05244937539100647,
0.1420670598745346,
0.04953403025865555,
0.048350390046834946,
0.05369783565402031,
0.009488415904343128,
0.0064998809248209,
-0.051613699644804,
0.005674134939908981,
0.034188635647296906,
0.013385728001594543,
0.08263807743787766,
-0.018379680812358856,
0.008900350891053677,
-0.0031315747182816267,
-0.017738593742251396,
0.02969249337911606,
-0.009794682264328003,
-0.0003795413940679282,
-0.039135731756687164,
-0.010658515617251396,
-0.015660222619771957,
-0.0639939084649086,
0.05121610686182976,
-0.03511963412165642,
-0.008420390076935291,
-0.06613576412200928,
0.04145084694027901,
0.04805609583854675,
-0.04022814705967903,
-0.032764218747615814,
0.003654992440715432,
0.014712290838360786,
0.04253076761960983,
-0.01347745954990387,
-0.016281787306070328,
-0.0008934778743423522,
0.016384785994887352,
0.00933013390749693,
0.05863587558269501,
0.00290188891813159,
-0.04375457018613815,
0.07083497196435928,
-0.006856921594589949
] |
PMID:18478
|
Hypopotassemia following open heart surgery by cardio-pulmonary bypass.
|
1. In this study where potassium concentration of perfusate of heart-lung machine was made high and no potassium was given until two hours after by-pass, the fall of plasma potassium level due to heart-lung bypass occurred in two stages, namely one hour of complete bypass and two hours after the end of bypass. 2. Regarding to the decrease of these plasma potassium level, increase of potassium excretion into urine and the movement of potassium from extracellular fluid into cells are presumed to be causes. Their genesis was discussed. 3. To prevent hypopotassemia following bypass, it is recommended to add KCl in the perfusate of heart-lung machine and to give intravenous drip of KCl immediately after bypass.
|
Hypopotassemia following open heart surgery by cardio-pulmonary bypass. 1. In this study where potassium concentration of perfusate of heart-lung machine was made high and no potassium was given until two hours after by-pass, the fall of plasma potassium level due to heart-lung bypass occurred in two stages, namely one hour of complete bypass and two hours after the end of bypass. 2. Regarding to the decrease of these plasma potassium level, increase of potassium excretion into urine and the movement of potassium from extracellular fluid into cells are presumed to be causes. Their genesis was discussed. 3. To prevent hypopotassemia following bypass, it is recommended to add KCl in the perfusate of heart-lung machine and to give intravenous drip of KCl immediately after bypass.
|
[
-0.016948537901043892,
0.016041813418269157,
0.04186859354376793,
0.04974693804979324,
-0.040144044905900955,
-0.05192697420716286,
0.032208364456892014,
0.042656365782022476,
-0.01635146141052246,
0.021900273859500885,
-0.02932298183441162,
-0.05387616530060768,
0.004441398195922375,
0.01438896544277668,
0.009352849796414375,
-0.02936055324971676,
0.01281728409230709,
-0.01742449775338173,
-0.1347908079624176,
0.015088190324604511,
0.01870357245206833,
0.03524627909064293,
0.02817872352898121,
0.0011646110797300935,
0.03055122308433056,
0.010489335283637047,
-0.030591510236263275,
-0.00797574408352375,
-0.028008153662085533,
-0.17612767219543457,
-0.021379202604293823,
0.001146146678365767,
0.038107458502054214,
0.03625015914440155,
-0.035756830126047134,
0.025369107723236084,
-0.019340267404913902,
0.04954998567700386,
-0.03571585938334465,
0.06329279392957687,
0.039238933473825455,
-0.04369618743658066,
-0.011166506446897984,
0.011603472754359245,
0.05092542991042137,
-0.002130813430994749,
0.04408770427107811,
-0.012268904596567154,
0.1450548619031906,
0.06602263450622559,
-0.003827106673270464,
0.05972616374492645,
0.026888161897659302,
0.07115969061851501,
0.0036410035099834204,
-0.037516068667173386,
0.040975023061037064,
0.0547855980694294,
0.03099166229367256,
-0.011672021821141243,
-0.007441062945872545,
0.029408523812890053,
-0.1305522471666336,
0.03148815408349037,
0.00525440601631999,
0.004703093320131302,
-0.010251707397401333,
-0.08417791873216629,
0.062327027320861816,
0.026765912771224976,
0.0014151246286928654,
0.05508742481470108,
0.01907442882657051,
-0.002711452776566148,
-0.020033210515975952,
0.025390025228261948,
0.0007749311625957489,
-0.03371232748031616,
0.0027613251004368067,
0.009231400676071644,
-0.03694883733987808,
-0.03388592600822449,
-0.015887022018432617,
-0.006474076770246029,
-0.025119150057435036,
-0.0432206392288208,
0.03668246418237686,
-0.03978325054049492,
-0.016930172219872475,
0.011724990792572498,
-0.0006232283776625991,
-0.08311501145362854,
-0.03312736004590988,
0.039038319140672684,
-0.08004789799451828,
-0.002514456631615758,
0.008085565641522408,
-0.008191430009901524,
-0.012495294213294983,
0.36684876680374146,
0.0026907972060143948,
0.02253677323460579,
-0.03974565863609314,
0.030825916677713394,
0.0202432032674551,
-0.11605505645275116,
0.034171558916568756,
0.036071810871362686,
0.014977606944739819,
0.006590923294425011,
0.0631701871752739,
-0.006249476224184036,
0.10724866390228271,
-0.01217526663094759,
0.00209441757760942,
0.09902369976043701,
0.01946047879755497,
0.050790105015039444,
-0.028479956090450287,
0.08713076263666153,
-0.060692522674798965,
0.004194499924778938,
-0.022093435749411583,
-0.024076152592897415,
0.04320874065160751,
0.0045431992039084435,
-0.009980431757867336,
0.08803045004606247,
0.019461629912257195,
-0.008473999798297882,
0.07312420010566711,
-0.009006206877529621,
-0.0025524734519422054,
-0.05493457615375519,
-0.05065862834453583,
-0.048494838178157806,
0.018958257511258125,
-0.009801981039345264,
0.04110226035118103,
-0.06336025148630142,
0.028354741632938385,
-0.04367655888199806,
-0.02766444906592369,
-0.07107619196176529,
0.011069892905652523,
0.06079944223165512,
0.012961111962795258,
-0.018018165603280067,
-0.023328030481934547,
-0.05456911399960518,
-0.019255487248301506,
0.036690063774585724,
-0.019882667809724808,
0.044931601732969284,
-0.024606699123978615,
0.01561825443059206,
-0.004969110246747732,
0.029384464025497437,
0.04470906779170036,
0.033998433500528336,
-0.0763702318072319,
-0.11463160812854767,
-0.028275560587644577,
0.026086842641234398,
0.04844838008284569,
0.039154715836048126,
0.008207126520574093,
-0.023921648040413857,
-0.01752585358917713,
0.02231716364622116,
-0.01890166662633419,
0.03632460534572601,
-0.03354727104306221,
-0.0517565943300724,
0.0658596009016037,
0.0006009392673149705,
0.008286412805318832,
-0.006864978466182947,
-0.04429876431822777,
-0.09705409407615662,
0.014247178100049496,
0.00960155576467514,
-0.013626866042613983,
0.006161974277347326,
-0.0010971681913360953,
-0.05013598874211311,
-0.05898439139127731,
0.04513555392622948,
0.07986738532781601,
0.06584953516721725,
-0.09667552262544632,
-0.005524746607989073,
-0.09202089160680771,
-0.0021807115990668535,
-0.019624847918748856,
-0.11605159193277359,
-0.07777046412229538,
0.034226421266794205,
0.045305974781513214,
-0.03197206184267998,
0.04483339935541153,
-0.026427127420902252,
0.010954838246107101,
0.031582631170749664,
-0.02335733361542225,
0.04685460776090622,
0.0012160688638687134,
-0.019063591957092285,
0.05332145094871521,
-0.023425409570336342,
0.0034521266352385283,
0.015092040412127972,
-0.01544139627367258,
0.0249429140239954,
0.018323080614209175,
-0.004940924234688282,
-0.011643504723906517,
-0.015695035457611084,
0.022433307021856308,
-0.0447939857840538,
0.05025124549865723,
-0.0020597500260919333,
-0.0025146622210741043,
-0.3219132125377655,
-0.046648506075143814,
-0.013228172436356544,
0.01616312935948372,
0.033189523965120316,
-0.028107333928346634,
0.014944493770599365,
-0.05165952071547508,
-0.022072218358516693,
-0.09137732535600662,
0.07941976934671402,
0.02027444913983345,
-0.09117472916841507,
0.01261327974498272,
0.0648609921336174,
0.0489550456404686,
0.03551119565963745,
-0.0377349779009819,
-0.0023329686373472214,
0.010841883718967438,
0.013540868647396564,
0.00866186898201704,
0.021541515365242958,
0.036373432725667953,
0.0012885329779237509,
0.006924001965671778,
0.10820665955543518,
-0.0375930517911911,
0.029693983495235443,
-0.03139431029558182,
-0.013138532638549805,
0.029919909313321114,
0.01030352059751749,
-0.08300027996301651,
0.02543225884437561,
-0.05231932923197746,
0.02139974944293499,
-0.007520212326198816,
-0.03080020844936371,
0.021943582221865654,
-0.08429078012704849,
0.02842876873910427,
-0.03548301011323929,
-0.0069715227000415325,
-0.055941350758075714,
-0.03292357176542282,
-0.03190760314464569,
0.06100757047533989,
-0.01113260630518198,
-0.016387036070227623,
0.025508476421236992,
0.0009244110551662743,
0.008273554034531116,
-0.030534006655216217,
0.05450894311070442,
-0.03659122437238693,
-0.018282489851117134,
-0.01838383637368679,
-0.0828060656785965,
-0.0034664447885006666,
0.027540292590856552,
-0.0012695557670667768,
-0.05769781768321991,
0.013386676087975502,
-0.036499883979558945,
0.024774406105279922,
0.019338125362992287,
0.05726008489727974,
0.04823172092437744,
-0.019847024232149124,
-0.016484394669532776,
0.07041574269533157,
-0.03081052005290985,
-0.067894846200943,
0.10225526243448257,
0.044586073607206345,
-0.05506829172372818,
-0.02445141412317753,
-0.01802763342857361,
0.007472989149391651,
0.07355517148971558,
-0.054878152906894684,
0.04265706613659859,
0.012990770861506462,
-0.02217678539454937,
0.07478585839271545,
-0.044336892664432526,
-0.03383126109838486,
-0.05567234009504318,
-0.05257066711783409,
-0.01974698342382908,
0.01036250963807106,
-0.007043006829917431,
0.014031988568603992,
0.06964243203401566,
-0.002816087333485484,
-0.21813847124576569,
0.017508985474705696,
0.04145030304789543,
-0.06288783997297287,
0.032881394028663635,
0.004918621387332678,
0.02804323472082615,
-0.02877730503678322,
-0.013654103502631187,
0.025739436969161034,
-0.06181526929140091,
0.02093801647424698,
0.048355769366025925,
0.04382701218128204,
-0.019536111503839493,
0.022099357098340988,
0.05457025766372681,
0.008378341794013977,
-0.008715991862118244,
0.009097442030906677,
0.003383616916835308,
-0.01289786770939827,
0.1156417652964592,
-0.015695134177803993,
-0.06489074975252151,
0.03002355247735977,
0.07319048792123795,
0.04224757105112076,
-0.03092770278453827,
0.028692690655589104,
0.04061558470129967,
0.00246063363738358,
0.03643312677741051,
0.00244104559533298,
0.02663118951022625,
-0.02542153187096119,
-0.030140146613121033,
0.044379811733961105,
0.05454059690237045,
0.017226936295628548,
0.00990799069404602,
-0.04460831731557846,
-0.002507653785869479,
-0.09602866321802139,
0.06934744119644165,
-0.03425290435552597,
-0.01758440025150776,
-0.0750698372721672,
0.04283725097775459,
-0.030763380229473114,
0.008632965385913849,
0.0017903459956869483,
0.05067844316363335,
0.036546673625707626,
0.016058439388871193,
0.029807772487401962,
-0.03031577728688717,
-0.009630974382162094,
0.03633486479520798,
0.0015259021893143654,
0.0146100465208292,
-0.032159075140953064,
-0.01888771541416645,
0.06138493865728378,
0.030436299741268158
] |
PMID:18481
|
Renal enzymes in kidney cells selected by D-valine medium.
|
The ability of D-valine medium to inhibit fibroblasts in cultures derived from kidneys of various mammals has enabled the selective proliferation of epithlial cells in the absence of fibroblast overgrowth. Studies of these selected epithelial cells have demonstrated the presence of D-amino acid oxidase, carbonic anhydrase, high levels of alkaline phosphatase and the renal specific pattern of lactate dehydrogenase. The presence of these renal enzymes suggests that the selected epithelial cells are of renal tubular origin and indicates that these differentiated functions are retained in cultured cells.
|
Renal enzymes in kidney cells selected by D-valine medium. The ability of D-valine medium to inhibit fibroblasts in cultures derived from kidneys of various mammals has enabled the selective proliferation of epithlial cells in the absence of fibroblast overgrowth. Studies of these selected epithelial cells have demonstrated the presence of D-amino acid oxidase, carbonic anhydrase, high levels of alkaline phosphatase and the renal specific pattern of lactate dehydrogenase. The presence of these renal enzymes suggests that the selected epithelial cells are of renal tubular origin and indicates that these differentiated functions are retained in cultured cells.
|
[
-0.02862480841577053,
-0.012928597629070282,
0.009069838561117649,
0.02592393383383751,
-0.03056040219962597,
-0.0512692853808403,
0.05577947944402695,
0.052864447236061096,
-0.03403344377875328,
-0.0018652795115485787,
0.02869480662047863,
-0.0573287233710289,
0.02928500436246395,
0.01894393377006054,
-0.005985158029943705,
-0.013091870583593845,
0.021189328283071518,
-0.019932541996240616,
-0.1189485415816307,
-0.04361845180392265,
0.03409320116043091,
0.017988137900829315,
0.052819691598415375,
-0.07023193687200546,
0.09658204764127731,
-0.03673461452126503,
0.032658133655786514,
-0.0033229824621230364,
-0.009243221022188663,
-0.1472926139831543,
0.006279201712459326,
-0.04143010079860687,
-0.02532530575990677,
-0.03604915365576744,
-0.025730906054377556,
-0.0036106719635427,
-0.006681940518319607,
0.005338534712791443,
-0.004570024088025093,
0.018349166959524155,
-0.03786749765276909,
-0.04699452966451645,
-0.011512333527207375,
0.025569427758455276,
-0.06331414729356766,
0.05973648652434349,
-0.036351047456264496,
0.024020999670028687,
0.07197058945894241,
-0.009749390184879303,
0.012897057458758354,
-0.025056753307580948,
0.013565532863140106,
-0.011209570802748203,
0.03831963986158371,
0.0012470432557165623,
0.045998845249414444,
0.06551098823547363,
0.00672462023794651,
0.037557829171419144,
0.0022016456350684166,
0.01140637043863535,
-0.1009814664721489,
0.06300852447748184,
0.013783658854663372,
0.046659160405397415,
0.012893063947558403,
-0.08722706139087677,
0.0344897024333477,
-0.03717022016644478,
-0.04919232800602913,
0.0018193746218457818,
0.0460117869079113,
-0.016101721674203873,
0.023290585726499557,
-0.0628681406378746,
-0.020570751279592514,
0.03356624022126198,
0.0467994287610054,
0.046731989830732346,
0.024125322699546814,
-0.018816743046045303,
0.04044594243168831,
-0.04108411446213722,
-0.04383866861462593,
0.014287585392594337,
0.049118440598249435,
-0.023202193900942802,
0.008895903825759888,
-0.037835586816072464,
-0.0410609170794487,
-0.0038049498107284307,
-0.04310246556997299,
-0.010930193588137627,
-0.0556219182908535,
0.02196718007326126,
0.06830286234617233,
-0.019113637506961823,
0.006315106060355902,
0.367850124835968,
-0.059854693710803986,
-0.005120598245412111,
-0.06593852490186691,
-0.0030802853871136904,
-0.03787731006741524,
-0.03101857379078865,
-0.057805903255939484,
-0.024872848764061928,
0.02606242150068283,
0.03221737593412399,
0.05698534473776817,
-0.00019957161566708237,
-0.0020245457999408245,
-0.07303792983293533,
-0.07649858295917511,
0.03347179293632507,
0.02251589670777321,
-0.010646958835422993,
-0.03845071420073509,
0.09125581383705139,
-0.011929990723729134,
-0.008759750984609127,
0.01409014780074358,
-0.0414777547121048,
0.040543753653764725,
0.009852426126599312,
0.011826843954622746,
0.0399879552423954,
0.04778577759861946,
0.030369682237505913,
-0.009470035322010517,
0.04101075232028961,
0.0113929882645607,
-0.027736904099583626,
-0.001435774494893849,
-0.02184821479022503,
-0.0009077771683223546,
-0.0797976478934288,
-0.050151485949754715,
-0.041778564453125,
-0.0746522918343544,
-0.010272211395204067,
-0.06369192153215408,
-0.03038148395717144,
-0.025938695296645164,
0.04846474528312683,
0.06936044991016388,
0.01915060356259346,
0.0062745846807956696,
-0.010251608677208424,
0.005755716003477573,
0.05805565416812897,
0.031467847526073456,
0.08057907968759537,
0.04943505674600601,
0.00013963208766654134,
-0.072902150452137,
-0.006642462220042944,
0.007718321401625872,
-0.014185496605932713,
-0.02164178155362606,
-0.048336442559957504,
0.013112551532685757,
0.10756669193506241,
0.00041601184057071805,
0.009744768962264061,
-0.05393903702497482,
0.013612065464258194,
0.04685138165950775,
-0.01698436215519905,
-0.012686526402831078,
-0.031005943194031715,
-0.03200829029083252,
-0.007835021242499352,
-0.025273697450757027,
-0.021384762600064278,
-0.04823775589466095,
0.01579132117331028,
-0.02537248097360134,
0.010919004678726196,
0.045679979026317596,
-0.06642504781484604,
-0.01152121927589178,
0.013254488818347454,
0.021708518266677856,
-0.07280949503183365,
-0.05639246106147766,
-0.008508452214300632,
0.05895266681909561,
0.05843929946422577,
0.0001338828296866268,
0.03288501128554344,
-0.058445751667022705,
-0.07873787730932236,
-0.0034215510822832584,
-0.03327802196145058,
-0.05413622781634331,
0.03879518061876297,
-0.031952958554029465,
0.006630075629800558,
0.027960777282714844,
0.05288593843579292,
0.017003517597913742,
0.017306923866271973,
0.012250357307493687,
0.014167395420372486,
0.014715533703565598,
0.027741797268390656,
0.023829972371459007,
-0.00867703277617693,
-0.12418822199106216,
-0.023223215714097023,
-0.02237359993159771,
-0.03553897514939308,
-0.052360277622938156,
0.03613598272204399,
-0.00700804078951478,
0.006674167234450579,
0.03737224265933037,
0.001318124239332974,
0.06505756825208664,
-0.03140819072723389,
-0.01555012445896864,
-0.2991704046726227,
-0.0022447723895311356,
-0.010349235497415066,
-0.016011357307434082,
0.08420473337173462,
-0.054643984884023666,
0.031283020973205566,
-0.017709700390696526,
-0.02696501836180687,
0.018355166539549828,
0.041305117309093475,
0.08880195766687393,
-0.02050240896642208,
-0.03934777155518532,
0.011854439973831177,
0.016073379665613174,
0.01632305048406124,
-0.04930892959237099,
0.01568068191409111,
-0.01341323833912611,
0.06226101145148277,
-0.030765853822231293,
0.02080162800848484,
0.05686942860484123,
0.07001242786645889,
-0.019877085462212563,
0.10554206371307373,
0.018292225897312164,
0.010093730874359608,
-0.024190867319703102,
0.03322352468967438,
0.045351747423410416,
-0.010358672589063644,
-0.06437083333730698,
0.006931116338819265,
-0.01178670208901167,
-0.020950600504875183,
-0.027102287858724594,
0.024624774232506752,
-0.01841019280254841,
-0.014567780308425426,
0.028627214953303337,
-0.10288336873054504,
-0.0537552572786808,
0.029825670644640923,
-0.0629226565361023,
0.019927624613046646,
0.06282118707895279,
0.003894309513270855,
-0.07768582552671432,
0.06485718488693237,
0.020508108660578728,
-0.010567406192421913,
-0.022755784913897514,
-0.00516518484801054,
0.013920620083808899,
-0.032268226146698,
-0.008823386393487453,
-0.0895550400018692,
-0.04259679466485977,
-0.026949137449264526,
0.06517307460308075,
-0.0008321330533362925,
0.031194712966680527,
-0.016478609293699265,
0.03296481817960739,
-0.03227626904845238,
0.023974131792783737,
0.06051388755440712,
0.019549226388335228,
-0.004500140901654959,
0.07959559559822083,
-0.021147077903151512,
0.10212602466344833,
0.061325881630182266,
0.04849858954548836,
-0.02979912795126438,
0.009398670867085457,
-0.05359727144241333,
0.028491146862506866,
0.0007404076750390232,
-0.09322548657655716,
0.019826514646410942,
-0.0005724243819713593,
0.007538029924035072,
0.08132881671190262,
0.04560168460011482,
0.015096079558134079,
0.01393195427954197,
-0.010616112500429153,
0.04384124279022217,
0.07196369022130966,
-0.048872191458940506,
-0.02066897042095661,
0.021106835454702377,
-0.01412487868219614,
-0.2578551769256592,
-0.0011775557650253177,
0.040546953678131104,
-0.04908313229680061,
-0.008378468453884125,
0.06703785806894302,
-0.029876625165343285,
0.005631750449538231,
0.03830927982926369,
0.05482405051589012,
0.05972249433398247,
-0.022139163687825203,
0.07363416254520416,
0.016023380681872368,
0.0032749250531196594,
0.04429517313838005,
0.09563368558883667,
-0.015685133635997772,
0.0248119980096817,
-0.005498345009982586,
0.04923275485634804,
-0.052188288420438766,
0.13488514721393585,
-0.03902481123805046,
0.002623538486659527,
0.04461461678147316,
-0.022532181814312935,
0.005681848619133234,
-0.09643580764532089,
-0.009177612140774727,
0.006934902630746365,
0.039668530225753784,
0.06587683409452438,
-0.03299367055296898,
0.04011797159910202,
-0.006476348266005516,
0.025689929723739624,
0.021955139935016632,
-0.03578508272767067,
-0.04131804779171944,
-0.023687519133090973,
-0.0005806193221360445,
-0.13763628900051117,
0.01157178170979023,
0.03258257731795311,
-0.06720688939094543,
-0.03293311968445778,
-0.03608526289463043,
0.07537567615509033,
-0.01638832315802574,
0.000530516728758812,
-0.01766812615096569,
-0.01741357333958149,
0.06434358656406403,
0.011499601416289806,
0.013481777161359787,
0.010498953983187675,
-0.0065125455148518085,
0.03651421144604683,
-0.011716747656464577,
0.013377971015870571,
0.015704723075032234,
0.029922641813755035,
0.14277154207229614,
0.000645697524305433
] |
PMID:18483
|
Interaction of local anesthetics with the transport system of glucose in human erythrocytes.
|
Local anesthetics inhibit the exhange transport of glucose in human erythroytes. All compounds tested showed a competitive inhibition except lidocaine and baycaine causing a non-competitive one. Moreover the transport system can bind two inhibitor molecules to one transport site as described for tetracaine and oxybuprocaine.
|
Interaction of local anesthetics with the transport system of glucose in human erythrocytes. Local anesthetics inhibit the exhange transport of glucose in human erythroytes. All compounds tested showed a competitive inhibition except lidocaine and baycaine causing a non-competitive one. Moreover the transport system can bind two inhibitor molecules to one transport site as described for tetracaine and oxybuprocaine.
|
[
-0.03332049772143364,
-0.0013762080343440175,
-0.01519369799643755,
0.06241658702492714,
-0.023530492559075356,
-0.004349225200712681,
0.09488711506128311,
-0.03081407956779003,
-0.028916627168655396,
-0.029588334262371063,
-0.03572651371359825,
-0.034654367715120316,
-0.03776416927576065,
0.042517632246017456,
-0.04012684151530266,
0.03388114646077156,
-0.028591841459274292,
0.014842048287391663,
-0.10772933810949326,
-0.041491493582725525,
0.009966130368411541,
0.03226601332426071,
-0.04498765245079994,
-0.00003223402381991036,
-0.004552870057523251,
0.014260451309382915,
0.023409785702824593,
-0.0031098874751478434,
-0.05979033187031746,
-0.19219852983951569,
-0.052126601338386536,
0.003484775312244892,
0.0013826084323227406,
-0.010471483692526817,
-0.054929595440626144,
0.03824562579393387,
-0.03908271715044975,
-0.002694562776014209,
-0.014217879623174667,
0.08289504051208496,
-0.0046247392892837524,
0.02870086021721363,
0.0008832757011987269,
0.056232597678899765,
-0.018296774476766586,
0.033579885959625244,
-0.028836209326982498,
0.008396252058446407,
0.11131491512060165,
0.0415983721613884,
0.04999399185180664,
0.03821602463722229,
0.06745751947164536,
0.03315554931759834,
-0.0485524907708168,
-0.03319978713989258,
-0.013254682533442974,
0.054445091634988785,
-0.045192036777734756,
0.026716848835349083,
0.02082197368144989,
0.07467599958181381,
-0.14076876640319824,
0.03870502486824989,
0.07544897496700287,
-0.059516165405511856,
0.03775942325592041,
0.033220142126083374,
0.02716832421720028,
0.007422563619911671,
-0.04153136909008026,
0.001111411489546299,
-0.018435854464769363,
0.030547842383384705,
0.003964981995522976,
-0.023808902129530907,
0.03955122455954552,
0.04249675199389458,
0.0018651270074769855,
-0.02115704119205475,
0.008639899082481861,
-0.06693985313177109,
-0.008199977688491344,
-0.047886308282613754,
0.015229232609272003,
-0.041786059737205505,
0.05637580156326294,
-0.03927259519696236,
-0.011658566072583199,
-0.04520247504115105,
-0.00769600085914135,
-0.06257884204387665,
-0.01719003915786743,
-0.00043526312219910324,
-0.07160031050443649,
-0.005783263593912125,
0.005452233366668224,
-0.019685011357069016,
-0.0005819304496981204,
0.35542628169059753,
0.0011234189150854945,
0.04528340324759483,
-0.03451087698340416,
0.014355935156345367,
-0.00633945083245635,
-0.07355204969644547,
0.04678531363606453,
-0.01373347919434309,
-0.014906494878232479,
0.010871845297515392,
0.09122859686613083,
0.03403591364622116,
0.015415721572935581,
0.02057608962059021,
-0.02713007666170597,
0.05212700366973877,
0.03588785603642464,
0.05004868656396866,
-0.014450776390731335,
0.014170358888804913,
-0.05661603808403015,
-0.0014577573165297508,
0.03872457519173622,
0.02214479073882103,
0.057489436119794846,
0.009776328690350056,
-0.04049167037010193,
0.10485131293535233,
-0.005373516585677862,
-0.012172938324511051,
0.07983355969190598,
0.056173745542764664,
-0.015118343755602837,
-0.0028656357899308205,
-0.033745184540748596,
0.03915707394480705,
0.024736948311328888,
-0.03582208231091499,
0.023452987894415855,
-0.052677009254693985,
0.004276474006474018,
-0.06382711976766586,
-0.00871712900698185,
-0.1056336760520935,
-0.020921800285577774,
0.05939355865120888,
0.04222949221730232,
-0.01727103814482689,
-0.043427422642707825,
0.010223430581390858,
-0.030909189954400063,
0.009214295074343681,
-0.003073655301705003,
-0.003937886096537113,
-0.06554511189460754,
0.03719935193657875,
0.010544215328991413,
-0.015434195287525654,
-0.024758491665124893,
-0.011917568743228912,
-0.011506825685501099,
-0.021644631400704384,
-0.05448688194155693,
0.07284912467002869,
-0.004590584430843592,
0.027361474931240082,
-0.013360981829464436,
-0.0386197529733181,
0.0396609753370285,
0.029848890379071236,
-0.0064381747506558895,
-0.03614848107099533,
0.01680714450776577,
-0.01454421877861023,
0.001036283327266574,
0.016757575795054436,
0.005813193041831255,
-0.018286747857928276,
0.01700684241950512,
-0.026928536593914032,
0.0065550473518669605,
0.03436185047030449,
-0.013759061694145203,
-0.0059328810311853886,
0.0563950352370739,
0.007025241851806641,
-0.04123205691576004,
-0.029672134667634964,
-0.016352156177163124,
0.05719522386789322,
-0.010631948709487915,
0.02418428659439087,
-0.07803056389093399,
-0.004617907572537661,
-0.014996988698840141,
-0.02544509433209896,
-0.00304021337069571,
-0.03986315801739693,
-0.02971155382692814,
0.0004753081011585891,
0.13206462562084198,
0.045110270380973816,
-0.04652378335595131,
0.06524275243282318,
0.043509017676115036,
-0.0024874533992260695,
-0.042929429560899734,
0.03726142644882202,
0.017338164150714874,
-0.03182631731033325,
-0.01777910813689232,
0.0089982058852911,
0.02398173324763775,
-0.014932992868125439,
-0.02856278046965599,
0.020739682018756866,
-0.007743775378912687,
0.03174680843949318,
0.02938329242169857,
-0.017648950219154358,
0.0714244619011879,
-0.022467851638793945,
0.020194269716739655,
-0.3432267904281616,
0.028394196182489395,
0.004215345252305269,
-0.0017182201845571399,
0.0626683384180069,
-0.0015087313950061798,
0.07688366621732712,
0.004893041215837002,
-0.06635025888681412,
-0.011126263067126274,
0.0773870050907135,
0.014012833125889301,
-0.055589769035577774,
0.018663164228200912,
-0.0009617991163395345,
0.01104744616895914,
0.030890120193362236,
-0.03437669575214386,
0.04236234351992607,
-0.0047875940799713135,
0.048611171543598175,
-0.045843515545129776,
-0.004136419389396906,
-0.0012402522843331099,
0.028508588671684265,
-0.03463410958647728,
0.09144636988639832,
-0.04384945333003998,
0.06930916756391525,
-0.055892590433359146,
0.00732134934514761,
0.060011327266693115,
-0.009456491097807884,
-0.09645010530948639,
-0.028711717575788498,
-0.013919775374233723,
0.034457214176654816,
-0.02800695039331913,
0.0679965689778328,
-0.0112616503611207,
-0.08955910056829453,
0.010063085705041885,
-0.025588572025299072,
-0.0031358646228909492,
-0.03687712550163269,
0.0034885304048657417,
-0.029286187142133713,
-0.04081070423126221,
-0.020125821232795715,
-0.034667495638132095,
0.038287099450826645,
0.04237782955169678,
0.04191476106643677,
-0.04881769046187401,
0.07675859332084656,
-0.04297277703881264,
-0.017298046499490738,
-0.0005946090095676482,
-0.058287542313337326,
0.0399797298014164,
0.016156131401658058,
0.0402996651828289,
0.00655946834012866,
-0.0149031151086092,
-0.021018894389271736,
0.006906857714056969,
-0.011368551291525364,
-0.03276965767145157,
0.10481391102075577,
0.03447679057717323,
0.037480033934116364,
0.16433373093605042,
-0.0529312826693058,
-0.007894325070083141,
0.06170593202114105,
0.0570259653031826,
0.044106192886829376,
0.004109697416424751,
-0.06508181989192963,
0.030751172453165054,
-0.00253420346416533,
0.007014926057308912,
0.06372757256031036,
-0.048376962542533875,
0.00671342620626092,
-0.04051193222403526,
0.07059727609157562,
-0.018986331298947334,
0.015913037583231926,
-0.0565849207341671,
0.04638461768627167,
-0.005600281525403261,
-0.05572366341948509,
-0.0642755851149559,
0.03703171759843826,
0.03896816819906235,
-0.23306213319301605,
0.015673641115427017,
0.031405139714479446,
-0.027176478877663612,
0.002455948619171977,
-0.023408230394124985,
-0.001980394357815385,
-0.03869941458106041,
-0.016683237627148628,
-0.05738658085465431,
0.009968381375074387,
-0.007023780141025782,
0.05739011988043785,
0.05918815732002258,
-0.033064715564250946,
0.030946912243962288,
0.0858442410826683,
0.01825777254998684,
0.011016681790351868,
-0.043985504657030106,
-0.020809652283787727,
-0.017446771264076233,
0.1311100423336029,
-0.027018599212169647,
0.0401226170361042,
0.04762321710586548,
0.016051754355430603,
0.010901262983679771,
-0.06293550133705139,
0.011084695346653461,
-0.0074818977154791355,
0.03629789873957634,
0.02677628956735134,
-0.06145375967025757,
0.015515143983066082,
0.010642104782164097,
-0.041403044015169144,
-0.01984696462750435,
0.015227270312607288,
-0.03324116766452789,
0.005603764206171036,
0.05320737138390541,
-0.0766901969909668,
-0.021099558100104332,
0.07152988761663437,
-0.04470064118504524,
0.00020163469889666885,
-0.08380458503961563,
0.03935392573475838,
0.004915338940918446,
0.04376384615898132,
-0.02397891692817211,
-0.023945806547999382,
-0.02953539416193962,
0.05274384096264839,
0.006036210339516401,
-0.039241258054971695,
-0.06323710083961487,
-0.030508175492286682,
0.03755610063672066,
-0.0151600893586874,
-0.05228377506136894,
-0.019016187638044357,
0.012947394512593746,
0.03945896774530411
] |
PMID:18484
|
Reversion in expression of hypoxanthine-guanine phosphoribosyltransferase in 6-thioguanine resistant neuroblastoma: evidence for reduced enzyme levels associated with unaltered catalytic activity.
|
Five clones of mouse neuroblastoma cells able to grow in hypoxanthine-aminopterin-thymidine containing medium were isolated from a hypoxanthine-guanine phosphoribosyltransferase (HGPRT; EC 2.4.2.8; IMP: pyrophosphate phosphoribosyltransferase) deficient cell line. These hypoxanthine-aminopterin-thymidine resistant revertant clone had 45-55% of wild-type cell HGPRT activity. Kinetic studies indicated that the HGPRT in revertant clones had a reduced maximal velocity as compared to wild type cells based on cell protein. Apparent Km values of HGPRT for hypoxanthine and 5-phosphoribosyl-1-pyrophosphate were similar in wild-type and revertant cells. Heat inactivation studies demonstrated a similar heat lability for HGPRT in revertant and wild-type cells. An antibody fraction prepared from serum of rabbits immunized with HGPRT partially purified from mouse liver was used to measure the amount of cross-reacting material in normal and revertant clones. The revertant clones had one-half the amounth of cross-reacting material present in wild-type cells, based on a given amount of cell protein. These data indicate that the revertant cells may contain fewer HGPRT molecules with unaltered catalytic activity.
|
Reversion in expression of hypoxanthine-guanine phosphoribosyltransferase in 6-thioguanine resistant neuroblastoma: evidence for reduced enzyme levels associated with unaltered catalytic activity. Five clones of mouse neuroblastoma cells able to grow in hypoxanthine-aminopterin-thymidine containing medium were isolated from a hypoxanthine-guanine phosphoribosyltransferase (HGPRT; EC 2.4.2.8; IMP: pyrophosphate phosphoribosyltransferase) deficient cell line. These hypoxanthine-aminopterin-thymidine resistant revertant clone had 45-55% of wild-type cell HGPRT activity. Kinetic studies indicated that the HGPRT in revertant clones had a reduced maximal velocity as compared to wild type cells based on cell protein. Apparent Km values of HGPRT for hypoxanthine and 5-phosphoribosyl-1-pyrophosphate were similar in wild-type and revertant cells. Heat inactivation studies demonstrated a similar heat lability for HGPRT in revertant and wild-type cells. An antibody fraction prepared from serum of rabbits immunized with HGPRT partially purified from mouse liver was used to measure the amount of cross-reacting material in normal and revertant clones. The revertant clones had one-half the amounth of cross-reacting material present in wild-type cells, based on a given amount of cell protein. These data indicate that the revertant cells may contain fewer HGPRT molecules with unaltered catalytic activity.
|
[
-0.04320564121007919,
0.003923802170902491,
-0.017589665949344635,
0.029469899833202362,
0.0028071950655430555,
0.027112586423754692,
0.04520547762513161,
0.04097669944167137,
-0.09675116091966629,
0.03759510815143585,
0.02666674181818962,
-0.07691940665245056,
-0.009120593778789043,
-0.031069034710526466,
-0.08853545039892197,
-0.03724818676710129,
-0.021653782576322556,
0.013583878986537457,
-0.011278110556304455,
0.0018015226814895868,
-0.027509931474924088,
-0.04541477560997009,
0.008120880462229252,
0.0075893220491707325,
0.030919503420591354,
0.0033263596706092358,
-0.039896007627248764,
0.06290438026189804,
-0.033830586820840836,
-0.1787339597940445,
0.04084496945142746,
-0.04701559245586395,
0.018632955849170685,
-0.0322466604411602,
0.011283284053206444,
0.03091341443359852,
-0.020766127854585648,
-0.00859996397048235,
-0.01899384707212448,
0.04599141702055931,
-0.029742710292339325,
-0.02232787013053894,
0.001719849999062717,
0.032561659812927246,
0.07068096101284027,
-0.02844996191561222,
-0.048199065029621124,
-0.01729677803814411,
0.0759008452296257,
0.01987024024128914,
0.029268747195601463,
-0.03110967017710209,
0.023436319082975388,
0.01659662462770939,
0.02133920229971409,
0.014399208128452301,
0.07382703572511673,
0.0403030700981617,
0.015458735637366772,
0.022219086065888405,
0.020433323457837105,
0.04941617324948311,
-0.12614332139492035,
0.014903558418154716,
0.07277493923902512,
-0.04208073392510414,
-0.008133189752697945,
-0.07838264107704163,
0.020888052880764008,
0.02008436806499958,
-0.07582952082157135,
0.018684102222323418,
0.012211057357490063,
0.007265699096024036,
-0.019779670983552933,
-0.015678979456424713,
-0.01581883803009987,
0.019057754427194595,
0.06933081895112991,
0.01604345440864563,
0.021902678534388542,
-0.0011920167598873377,
-0.00825495645403862,
-0.02907320111989975,
-0.008847548626363277,
-0.033220913261175156,
-0.02031717263162136,
-0.027861369773745537,
0.0029284420888870955,
0.01601036824285984,
-0.017736297100782394,
-0.05897628888487816,
0.017123421654105186,
0.01988668367266655,
-0.06596633791923523,
0.038400691002607346,
0.08259467035531998,
0.028232881799340248,
0.031203072518110275,
0.38768813014030457,
-0.011141515336930752,
0.007144237868487835,
-0.06812936812639236,
0.032761964946985245,
-0.01227262057363987,
-0.060139644891023636,
-0.015131446532905102,
-0.022605357691645622,
0.030171342194080353,
0.06547395884990692,
0.06266258656978607,
0.015922976657748222,
0.06336376070976257,
-0.05088989436626434,
0.00032921560341492295,
-0.026997368782758713,
0.016078686341643333,
0.04192187264561653,
-0.040859900414943695,
0.09907692670822144,
-0.005179180763661861,
0.005683195777237415,
0.03397414833307266,
-0.01571717858314514,
0.0491320975124836,
0.02028927579522133,
-0.03927149623632431,
0.08851448446512222,
0.09270778298377991,
0.032856639474630356,
0.008494500070810318,
0.07160136103630066,
-0.015599088743329048,
0.005366472061723471,
0.004870319273322821,
0.0017280357424169779,
0.016725610941648483,
-0.02499852143228054,
-0.0017228618962690234,
0.009788073599338531,
0.033060476183891296,
-0.0651576891541481,
-0.004935515113174915,
-0.03891284018754959,
-0.015567793510854244,
0.07955710589885712,
-0.022121626883745193,
0.05051855742931366,
0.000057259741879533976,
0.02239743247628212,
0.004295202903449535,
0.0962456464767456,
0.030338825657963753,
0.019641956314444542,
-0.04943212866783142,
0.03812070190906525,
-0.04909928888082504,
-0.005440379958599806,
0.014435739256441593,
0.021054420620203018,
-0.001696670544333756,
-0.017733976244926453,
0.016464315354824066,
0.050774361938238144,
0.04186692461371422,
-0.004462091717869043,
-0.0015881602885201573,
-0.001039285445585847,
-0.008753317408263683,
-0.040169697254896164,
-0.010580088943243027,
0.03248674422502518,
0.004989320412278175,
-0.09161510318517685,
-0.028848344460129738,
-0.04335053637623787,
-0.018338076770305634,
-0.035879261791706085,
0.02480686828494072,
-0.00016999102081172168,
0.03679869323968887,
-0.04145645722746849,
-0.0689552053809166,
0.03549377620220184,
-0.015478236600756645,
-0.03525171056389809,
-0.044888753443956375,
-0.0031135326717048883,
-0.00341266137547791,
0.06311461329460144,
-0.021585991606116295,
-0.02182365022599697,
-0.09957290440797806,
0.00009537085861666128,
-0.013705757446587086,
-0.0514058880507946,
-0.03104645200073719,
0.02972516417503357,
-0.005838348530232906,
-0.04487501084804535,
0.0928284153342247,
0.02352958917617798,
0.009786458685994148,
0.044240597635507584,
-0.015031895600259304,
0.05486509948968887,
-0.014812168665230274,
0.024570003151893616,
0.037307240068912506,
0.002503428841009736,
-0.032664284110069275,
-0.017446480691432953,
-0.0323348306119442,
0.04087275266647339,
-0.06060479208827019,
0.048687420785427094,
-0.02284199371933937,
0.034663401544094086,
0.07594989985227585,
0.005815237294882536,
0.007750358432531357,
-0.018110744655132294,
-0.009974236600100994,
-0.3114408254623413,
-0.09535481035709381,
0.01493509765714407,
-0.009549884125590324,
0.015253775753080845,
-0.02641117572784424,
0.014219813980162144,
-0.04152771458029747,
0.027217820286750793,
-0.02074894681572914,
0.09300244599580765,
0.0954144224524498,
-0.051867250353097916,
-0.03727886080741882,
-0.027468997985124588,
0.029501887038350105,
0.0365682877600193,
-0.01811586320400238,
0.04486685246229172,
0.02165280655026436,
-0.0006387719186022878,
-0.00662272609770298,
-0.006169060245156288,
-0.009072788991034031,
0.04805877432227135,
0.008793666958808899,
0.11375868320465088,
0.012813116423785686,
0.005146235227584839,
-0.024403629824519157,
0.02196807786822319,
0.04742100089788437,
-0.019098538905382156,
-0.07329846918582916,
0.024468395859003067,
0.014389597810804844,
0.018679415807127953,
0.00630226219072938,
-0.022055432200431824,
0.006397849880158901,
-0.0030374282505363226,
0.022249994799494743,
-0.008838275447487831,
-0.04325440153479576,
-0.03134632110595703,
-0.0020306757651269436,
-0.013004179112613201,
-0.01744614727795124,
0.0007486798567697406,
-0.021184897050261497,
0.03093462623655796,
0.01290715392678976,
-0.0002731755084823817,
-0.018452461808919907,
-0.001388162956573069,
0.030144067481160164,
-0.02283051423728466,
0.036517344415187836,
-0.057102929800748825,
-0.05691995844244957,
-0.02334142103791237,
0.0184831153601408,
-0.05557632073760033,
0.052523840218782425,
-0.029445596039295197,
0.007981319911777973,
0.06507022678852081,
-0.01587073691189289,
0.06462955474853516,
0.019868722185492516,
-0.010691766627132893,
0.0967923030257225,
0.026955028995871544,
0.02817557193338871,
-0.005479216109961271,
-0.035885024815797806,
-0.014390218071639538,
-0.004135301802307367,
-0.13346171379089355,
0.010289636440575123,
-0.0011919940588995814,
-0.023167839273810387,
0.013572607189416885,
0.031230896711349487,
-0.028220197185873985,
0.028711635619401932,
0.07267481088638306,
-0.044447001069784164,
0.035656824707984924,
0.042034607380628586,
0.022075653076171875,
0.006331809796392918,
-0.05466833710670471,
-0.05518745258450508,
0.03106045536696911,
0.004035096149891615,
-0.28653237223625183,
-0.04410768300294876,
-0.01728971116244793,
0.026129916310310364,
-0.033342763781547546,
0.0185625609010458,
0.06579265743494034,
-0.07292308658361435,
-0.04614882543683052,
0.06693851202726364,
0.020698795095086098,
0.02095094881951809,
0.063826784491539,
0.05445332080125809,
-0.011501072905957699,
0.004818687681108713,
0.03577260300517082,
-0.013504572212696075,
-0.004955206997692585,
-0.035103943198919296,
0.011948253028094769,
-0.04003823176026344,
0.13541485369205475,
-0.021008029580116272,
-0.028482062742114067,
0.019014107063412666,
-0.07038074731826782,
0.007585146930068731,
0.005401200149208307,
0.0027531529776751995,
-0.05133238434791565,
0.02294716238975525,
0.07461237162351608,
-0.05202067270874977,
0.054112665355205536,
0.04306812584400177,
-0.04191887006163597,
0.0005714214639738202,
-0.022545304149389267,
-0.028162535279989243,
-0.0370326042175293,
0.026745464652776718,
-0.07581403851509094,
-0.06362250447273254,
0.07747247070074081,
-0.09399955719709396,
-0.04925443232059479,
-0.042470306158065796,
-0.02842091955244541,
-0.03439076989889145,
-0.03196846693754196,
0.037441518157720566,
0.008495892398059368,
0.010256152600049973,
-0.009800691157579422,
-0.01816781982779503,
-0.031836286187171936,
-0.011291186325252056,
0.07003789395093918,
-0.018902229145169258,
-0.017001114785671234,
-0.002220848575234413,
-0.003803620347753167,
0.13824908435344696,
-0.023807421326637268
] |
PMID:18486
|
Analysis of medazepam, diazepam, and metabolites in plasma by gas-liquid chromatography with electrolytic conductivity detection.
|
An improved electrolytic conductivity detector allowed the gas-liquid chromatographic analysis of medazepam, diazepam, and major metabolites in 2 ml plasma at concentrations of 20 microgram/l. The detector had a sensitivity limit of less than 1 ng (or 100 pg nitrogen) when operated in the nitrogen-selective mode and a nitrogen/carbon elemental selectivity ratio of greater than 100,000 compared to octadecane and cholesterol. Detector response when operated in various element-selective chemical modes was investigated, and its application to the analysis of the title compounds was compared to electron capture and flame ionization detection systems.
|
Analysis of medazepam, diazepam, and metabolites in plasma by gas-liquid chromatography with electrolytic conductivity detection. An improved electrolytic conductivity detector allowed the gas-liquid chromatographic analysis of medazepam, diazepam, and major metabolites in 2 ml plasma at concentrations of 20 microgram/l. The detector had a sensitivity limit of less than 1 ng (or 100 pg nitrogen) when operated in the nitrogen-selective mode and a nitrogen/carbon elemental selectivity ratio of greater than 100,000 compared to octadecane and cholesterol. Detector response when operated in various element-selective chemical modes was investigated, and its application to the analysis of the title compounds was compared to electron capture and flame ionization detection systems.
|
[
-0.03631935641169548,
0.04645411670207977,
0.05427452176809311,
0.03081399016082287,
0.03962552547454834,
0.005077672656625509,
0.04110359027981758,
0.03893756866455078,
-0.09118827432394028,
-0.05782165005803108,
-0.04637642204761505,
0.0015660300850868225,
-0.047556906938552856,
0.02390994317829609,
0.0001937660708790645,
-0.01604638062417507,
0.08055505156517029,
-0.05309916287660599,
-0.015265746973454952,
0.002915938850492239,
0.009790935553610325,
0.014966263435781002,
0.03158605098724365,
-0.01011935155838728,
0.03788985311985016,
0.03815672919154167,
0.0008574254461564124,
-0.012063986621797085,
-0.06942183524370193,
-0.17800723016262054,
0.0437442846596241,
0.031099209561944008,
0.01196798775345087,
-0.06073032692074776,
-0.031583961099386215,
0.021980615332722664,
-0.006753031630069017,
0.02802337519824505,
-0.014395131729543209,
0.01017676666378975,
0.04681036248803139,
0.007339332718402147,
-0.0589928962290287,
-0.020925523713231087,
-0.0169809702783823,
-0.045901115983724594,
0.02273876778781414,
0.0033829521853476763,
0.11892596632242203,
-0.06396415084600449,
-0.007607020437717438,
0.024147292599081993,
0.09292232990264893,
0.033678606152534485,
0.01358656957745552,
-0.02268395386636257,
-0.0039269523695111275,
-0.04803385213017464,
-0.03812248259782791,
0.028851786628365517,
-0.027228090912103653,
0.048594146966934204,
-0.1212598904967308,
0.003978836815804243,
0.009531822986900806,
-0.004833941347897053,
-0.01279304176568985,
-0.05325644463300705,
0.041325896978378296,
0.01616237498819828,
-0.03984023258090019,
-0.019656719639897346,
-0.0488448441028595,
0.025486573576927185,
0.025837356224656105,
-0.006640143692493439,
0.04876374453306198,
-0.03456459194421768,
-0.030185135081410408,
-0.022275524213910103,
0.004506188444793224,
-0.014903268776834011,
0.03195391595363617,
-0.030026955530047417,
0.010155967436730862,
0.003485096385702491,
0.07575569301843643,
0.014705255627632141,
0.03250308707356453,
0.023554598912596703,
0.05436541140079498,
-0.018776802346110344,
-0.0707131028175354,
-0.02209009788930416,
-0.004790469538420439,
-0.0457877553999424,
0.002218755194917321,
0.012915000319480896,
-0.004436545539647341,
0.3989224135875702,
0.028837433084845543,
0.006516174878925085,
-0.07712331414222717,
0.012717947363853455,
-0.01359693706035614,
-0.02722027339041233,
-0.0031899153254926205,
0.003811249975115061,
-0.03638961538672447,
-0.02571140043437481,
0.08109106868505478,
-0.020883377641439438,
0.06444191187620163,
-0.05376853048801422,
-0.04386119544506073,
0.013893448747694492,
0.0011141864815726876,
0.0012972122058272362,
0.01780860126018524,
0.0281207375228405,
0.009503908455371857,
-0.021893396973609924,
0.007710675708949566,
-0.019316306337714195,
0.06490246951580048,
-0.027329009026288986,
-0.05076620727777481,
0.08625280857086182,
0.003881363198161125,
0.02565283328294754,
0.051071666181087494,
-0.0128652798011899,
0.03484293445944786,
-0.009094309993088245,
0.016442906111478806,
0.062237564474344254,
0.02815420925617218,
-0.00910615362226963,
0.04459495469927788,
0.004126858431845903,
-0.033477745950222015,
0.005026846658438444,
-0.014356330968439579,
-0.0974702313542366,
-0.082632876932621,
0.09197382628917694,
-0.014017905108630657,
-0.004746138583868742,
-0.0323156900703907,
-0.046383753418922424,
0.008516393601894379,
0.02772890403866768,
-0.02075563557446003,
0.04235003516077995,
-0.016761215403676033,
0.002367301844060421,
0.017147846519947052,
0.01768796145915985,
-0.041467055678367615,
0.031303610652685165,
-0.03252587467432022,
-0.02029373124241829,
0.01296662725508213,
0.1548849642276764,
-0.00759469997137785,
0.007635974790900946,
0.0042274873703718185,
-0.01684783399105072,
-0.020378518849611282,
0.012046371586620808,
0.038901038467884064,
0.04598401486873627,
-0.06579195708036423,
0.047786351293325424,
-0.06976447999477386,
-0.013984034769237041,
0.014220993034541607,
0.015344150364398956,
0.04154668375849724,
0.017110884189605713,
0.04165777936577797,
0.05890100076794624,
-0.038914747536182404,
0.0076207974925637245,
0.040208425372838974,
0.005599529482424259,
-0.027845509350299835,
-0.012485608458518982,
-0.009899072349071503,
0.06858565658330917,
-0.03186923637986183,
0.0019146979320794344,
-0.03036736324429512,
0.04474928230047226,
-0.06745974719524384,
-0.020824283361434937,
-0.053196027874946594,
0.02691509947180748,
-0.009547540917992592,
-0.041645459830760956,
0.0962882786989212,
0.01792222633957863,
0.0079755624756217,
0.04771025851368904,
0.05533824488520622,
0.03644184768199921,
-0.048047248274087906,
0.0073593915440142155,
-0.0038940785452723503,
0.003346604760736227,
0.028298646211624146,
-0.004889314994215965,
-0.00037846851046197116,
-0.004912223666906357,
-0.04310861974954605,
-0.01771119423210621,
-0.05595787242054939,
0.06944160908460617,
0.03614995256066322,
0.016257887706160545,
0.042521633207798004,
-0.03494901955127716,
-0.03297342360019684,
-0.3300269842147827,
-0.027840662747621536,
0.08436723053455353,
0.04277661815285683,
0.045636385679244995,
-0.026013223454356194,
0.050479572266340256,
-0.08444666117429733,
-0.01639041304588318,
-0.004325361922383308,
0.030911944806575775,
0.030799446627497673,
-0.04235510900616646,
0.0028903114143759012,
-0.01538851484656334,
0.01899898424744606,
-0.0021714537870138884,
-0.030829794704914093,
0.02724124677479267,
0.01815384440124035,
0.0007378699956461787,
0.0015193071449175477,
-0.013282008469104767,
0.06504747271537781,
0.06522166728973389,
-0.0019130451837554574,
0.105315200984478,
-0.010712951421737671,
-0.015868593007326126,
-0.07400083541870117,
0.03511589765548706,
0.03440415486693382,
-0.010371522046625614,
-0.07912247627973557,
-0.021872451528906822,
-0.053888145834207535,
-0.056664805859327316,
-0.02739684097468853,
0.005857399664819241,
-0.02418319135904312,
-0.0701630637049675,
-0.004205652978271246,
0.04754360392689705,
-0.09029722958803177,
-0.038467567414045334,
-0.06840338557958603,
-0.014332105405628681,
-0.07005903869867325,
-0.0407993420958519,
0.03985465690493584,
0.05895310267806053,
0.027437718585133553,
0.015132884494960308,
-0.04847025126218796,
0.06272725015878677,
-0.03801002725958824,
-0.044040750712156296,
-0.03485724329948425,
-0.04185120761394501,
-0.051659565418958664,
0.034395914524793625,
0.005425398703664541,
0.026453757658600807,
-0.03389236330986023,
0.010980179533362389,
0.013393130153417587,
0.05976368486881256,
0.02811460942029953,
0.06907769292593002,
-0.015497260726988316,
0.029692787677049637,
0.11261444538831711,
-0.026650069281458855,
-0.013319266960024834,
0.0634494200348854,
-0.011759721674025059,
-0.008686142973601818,
-0.041128695011138916,
-0.08074381202459335,
0.030794290825724602,
0.012533517554402351,
0.03411306440830231,
0.02784639783203602,
-0.009892361238598824,
-0.026489075273275375,
0.041804127395153046,
0.02612263150513172,
-0.021553216502070427,
-0.012619114480912685,
-0.01863938942551613,
0.004847004543989897,
0.028238432481884956,
-0.03483809530735016,
-0.05311780795454979,
0.013498318381607533,
0.016524383798241615,
-0.2715480923652649,
-0.027997391298413277,
-0.017799993976950645,
0.033479269593954086,
0.00913784559816122,
-0.047518983483314514,
0.0755457952618599,
-0.01059660967439413,
-0.020671861246228218,
-0.01717851683497429,
-0.05892162397503853,
-0.0006626269896514714,
-0.04523231461644173,
0.03856530785560608,
0.007867378182709217,
0.02657359465956688,
0.05782805010676384,
-0.04209808632731438,
-0.00006577484600711614,
0.02651301398873329,
0.04099979251623154,
-0.012739261612296104,
0.10961957275867462,
0.03924977779388428,
-0.043776411563158035,
0.0345865897834301,
0.028333649039268494,
-0.026615001261234283,
-0.016487466171383858,
-0.009044792503118515,
-0.004520054906606674,
0.012605997733771801,
0.030820205807685852,
-0.004779445007443428,
0.023033224046230316,
0.07288343459367752,
-0.00467564957216382,
-0.00626295804977417,
-0.010459493845701218,
-0.015996649861335754,
-0.006212565116584301,
0.008516627363860607,
-0.026266537606716156,
-0.06176115944981575,
0.04626351594924927,
-0.08502521365880966,
-0.012417640537023544,
-0.03348591551184654,
-0.0005709084216505289,
0.038468170911073685,
-0.05548963323235512,
-0.028505507856607437,
-0.012514329515397549,
-0.005784828681498766,
0.11395520716905594,
0.05051829665899277,
-0.03284546360373497,
0.013555482029914856,
0.024359388276934624,
-0.021041374653577805,
-0.04380985349416733,
0.020367218181490898,
-0.003460709471255541,
0.07619839161634445,
0.006271228194236755
] |
PMID:18487
|
Use of an amino-silica column for the high-performance liquid chromatographic analysis of synthetic oligodeoxy-nucleotides.
|
The use of an amino-silica column in the chromatographic analysis of synthetic oligodeoxyribonucleotides and their derivatives from different stages of oligonucleotide synthesis has been investigated. By eluting with 0.10 M potassium phosphate solution of pH 3.30, the nucleotide composition of oligonucleotides can be established within 15 min. In a linear gradient of phosphate buffer (0.10-0.75 M) at neutral pH, the separation of oligonucleotides by length and in an acidic medium pH 3.30-4.30) by composition is possible; the oligonucleotides may be in the free form or modified by the various protecting groups used in synthetic oligonucleotide chemistry. The analysis of some reaction mixtures from different stages of oligonucleotide synthesis and of a number of synthetic oligodeoxyribonucleotides and their derivatives has been performed.
|
Use of an amino-silica column for the high-performance liquid chromatographic analysis of synthetic oligodeoxy-nucleotides. The use of an amino-silica column in the chromatographic analysis of synthetic oligodeoxyribonucleotides and their derivatives from different stages of oligonucleotide synthesis has been investigated. By eluting with 0.10 M potassium phosphate solution of pH 3.30, the nucleotide composition of oligonucleotides can be established within 15 min. In a linear gradient of phosphate buffer (0.10-0.75 M) at neutral pH, the separation of oligonucleotides by length and in an acidic medium pH 3.30-4.30) by composition is possible; the oligonucleotides may be in the free form or modified by the various protecting groups used in synthetic oligonucleotide chemistry. The analysis of some reaction mixtures from different stages of oligonucleotide synthesis and of a number of synthetic oligodeoxyribonucleotides and their derivatives has been performed.
|
[
-0.049232929944992065,
0.0057114651426672935,
0.03890364617109299,
-0.04884284362196922,
0.049900513142347336,
0.038518790155649185,
0.018543807789683342,
0.027522528544068336,
-0.0531754270195961,
0.02013539709150791,
0.0254799984395504,
-0.03150256350636482,
0.0387522429227829,
0.020024757832288742,
-0.06507281213998795,
-0.05294867232441902,
-0.009289831854403019,
0.004121545236557722,
-0.017775608226656914,
-0.04393483325839043,
0.018223529681563377,
-0.016261324286460876,
-0.003860875964164734,
-0.03330063819885254,
0.011541105806827545,
0.08188846707344055,
0.015219432301819324,
-0.02358321286737919,
-0.04016400873661041,
-0.19461530447006226,
0.04306149110198021,
0.03655422478914261,
0.04691486060619354,
-0.04883392155170441,
-0.007503584958612919,
-0.018267802894115448,
-0.04100555554032326,
-0.04979284107685089,
-0.02539539523422718,
0.04299546033143997,
0.030572248622775078,
-0.027347182855010033,
-0.009734495542943478,
0.05929041653871536,
0.038179025053977966,
-0.011493354104459286,
-0.06418344378471375,
-0.0011597138363867998,
0.07034724950790405,
0.0014852536842226982,
-0.038157571107149124,
-0.01945919170975685,
0.029412753880023956,
-0.013364087790250778,
-0.04551245644688606,
0.00022592021559830755,
0.04044764116406441,
0.011057320050895214,
-0.0022807111963629723,
0.029216216877102852,
0.03077167086303234,
-0.0036917771212756634,
-0.15337516367435455,
0.09244315326213837,
0.050726182758808136,
0.020081056281924248,
0.05792373791337013,
-0.07025086879730225,
0.006831608712673187,
-0.0013174883788451552,
-0.07440044730901718,
0.029083138331770897,
0.030184729024767876,
0.01937248557806015,
0.05046336352825165,
0.013154394924640656,
0.029134254902601242,
-0.05998685583472252,
0.06889372318983078,
-0.0028712418861687183,
-0.00625681271776557,
0.0260564424097538,
0.02967659756541252,
-0.03313290700316429,
-0.06798294931650162,
0.04630854353308678,
-0.03313054144382477,
0.003265033010393381,
0.03804021701216698,
-0.0007742607267573476,
0.02115393988788128,
-0.01845812238752842,
-0.007999750785529613,
-0.00016638464876450598,
-0.05878469720482826,
0.0015843033324927092,
-0.010988091118633747,
0.004378854297101498,
0.09073784947395325,
0.3662327527999878,
-0.0017537323292344809,
0.0210716649889946,
-0.0323229655623436,
-0.038585495203733444,
-0.03984701260924339,
-0.03831568360328674,
-0.026255778968334198,
-0.05122225731611252,
0.060252971947193146,
0.044690340757369995,
0.019135795533657074,
0.039632562547922134,
0.025226177647709846,
-0.06461203098297119,
-0.015938973054289818,
-0.014089691452682018,
-0.03201182186603546,
-0.03506175056099892,
-0.0783599317073822,
0.048176392912864685,
-0.03723640739917755,
-0.025139497593045235,
-0.04196753725409508,
-0.0022303354926407337,
0.0380384661257267,
-0.009064367040991783,
-0.009062864817678928,
0.08613566309213638,
0.061910197138786316,
0.05230725556612015,
0.009376958012580872,
0.021987032145261765,
-0.0008560825954191387,
0.04467106983065605,
-0.000594126817304641,
-0.0025551714934408665,
-0.023080937564373016,
-0.03552858531475067,
0.05274540185928345,
0.0045159137807786465,
-0.030009768903255463,
-0.04608096554875374,
-0.0522494800388813,
-0.01788848452270031,
-0.03483940660953522,
0.08733483403921127,
-0.05569465458393097,
0.06676173955202103,
-0.006997920572757721,
0.002220749855041504,
0.001877536647953093,
0.07268799841403961,
-0.013382505625486374,
0.010090392082929611,
-0.03162889555096626,
-0.02902180328965187,
0.0001484998210798949,
-0.004780625458806753,
-0.056350260972976685,
-0.0056326440535485744,
-0.06806989759206772,
-0.03392845019698143,
0.032749440521001816,
0.09706509858369827,
-0.05891188234090805,
-0.04709627851843834,
-0.02413506805896759,
0.07916367053985596,
0.022074855864048004,
0.023467252030968666,
0.026684045791625977,
-0.03411521017551422,
-0.005094913765788078,
0.039079125970602036,
-0.062010664492845535,
-0.054067280143499374,
-0.022022811695933342,
0.041696835309267044,
0.04769783467054367,
-0.01073470525443554,
0.010481921955943108,
0.0007228653412312269,
-0.06792373210191727,
0.033837106078863144,
0.024085989221930504,
-0.09282601624727249,
-0.04767860844731331,
0.008028347976505756,
0.011115063913166523,
0.060938939452171326,
0.010118911042809486,
0.039845142513513565,
-0.04699196666479111,
0.07387358695268631,
0.019723283126950264,
-0.0019056415185332298,
-0.009040703997015953,
0.012420590035617352,
-0.0037449635565280914,
-0.009495511651039124,
0.10049626976251602,
0.024069899693131447,
-0.014483330771327019,
0.057545483112335205,
0.04238463193178177,
-0.013310748152434826,
0.02877594716846943,
-0.030169466510415077,
0.026529140770435333,
-0.007799332961440086,
-0.044394880533218384,
0.0068673742935061455,
-0.04382459446787834,
0.06746549904346466,
-0.05845300480723381,
-0.02575567550957203,
-0.006179281044751406,
0.031043078750371933,
0.06511421501636505,
0.008052950724959373,
-0.02003450132906437,
-0.017914341762661934,
-0.040135275572538376,
-0.29107338190078735,
-0.024651888757944107,
0.02982909046113491,
0.03880825638771057,
0.048635490238666534,
-0.044009312987327576,
0.018571963533759117,
0.0017390905413776636,
0.001431737095117569,
-0.00824923999607563,
0.03679240122437477,
0.048395007848739624,
-0.01833375170826912,
-0.045635886490345,
-0.020534414798021317,
0.0605803057551384,
0.01742989756166935,
-0.03804762288928032,
0.025931086391210556,
0.04251878708600998,
0.03979107365012169,
-0.0003898863506037742,
-0.029079528525471687,
0.028297070413827896,
0.09527624398469925,
-0.003002334153279662,
0.14675387740135193,
-0.027172915637493134,
0.013287797570228577,
0.023138299584388733,
0.012437534518539906,
-0.024187281727790833,
-0.0018117233412340283,
-0.0765262320637703,
-0.004909137263894081,
-0.04938945174217224,
-0.03596944734454155,
-0.014868427999317646,
0.029743000864982605,
-0.05822933837771416,
0.009098152630031109,
-0.020924033597111702,
0.01372451987117529,
-0.04355482757091522,
-0.003592503722757101,
-0.041865572333335876,
-0.018880464136600494,
0.024399522691965103,
0.0061599500477313995,
-0.0071587227284908295,
0.041346509009599686,
0.0122225321829319,
0.07545560598373413,
0.01107539888471365,
-0.018195373937487602,
0.02226356230676174,
-0.054372772574424744,
-0.040738340467214584,
-0.05449536442756653,
-0.06650148332118988,
0.061896342784166336,
0.025280769914388657,
-0.004981370642781258,
-0.007911678403615952,
-0.007329062558710575,
-0.00037881580647081137,
0.07017404586076736,
-0.027991866692900658,
0.05711441859602928,
-0.017713584005832672,
0.01970195397734642,
0.09185218811035156,
0.006282953545451164,
0.06142783164978027,
0.03496456518769264,
0.031788650900125504,
-0.059924621134996414,
-0.07456965744495392,
-0.06974898278713226,
0.01689615659415722,
0.04029934108257294,
-0.01510075107216835,
0.052710335701704025,
-0.020180970430374146,
-0.003652452025562525,
0.07950045168399811,
0.04116398096084595,
0.015438351780176163,
0.013951482251286507,
0.025871172547340393,
0.03258991986513138,
0.020959356799721718,
0.02083117887377739,
-0.018669448792934418,
0.04888677969574928,
0.018811853602528572,
-0.2952362298965454,
0.05454244092106819,
-0.014633599668741226,
0.022439762949943542,
0.014719018712639809,
0.0018197876634076238,
0.010831180028617382,
-0.03043288178741932,
-0.035687405616045,
0.03059462457895279,
-0.030926797538995743,
0.008954906836152077,
0.04227042943239212,
-0.05678004398941994,
-0.024380650371313095,
0.009750054217875004,
0.0653950646519661,
-0.0071299122646451,
0.010475282557308674,
-0.016730278730392456,
0.04254956915974617,
-0.07710284739732742,
0.1350584179162979,
0.011854665353894234,
-0.0675116628408432,
-0.041912779211997986,
0.04672924801707268,
0.05847645550966263,
-0.03980380669236183,
-0.0669502466917038,
0.03291531652212143,
0.004922675900161266,
0.019416416063904762,
0.0025160047225654125,
0.015983620658516884,
0.04077012091875076,
-0.023582084104418755,
0.0007071049767546356,
-0.051016174256801605,
-0.008601803332567215,
-0.043445032089948654,
-0.00828601699322462,
-0.10001800954341888,
-0.04466717317700386,
0.0524548701941967,
-0.07177694141864777,
-0.05499304085969925,
-0.07232673466205597,
0.013340423814952374,
-0.01223000232130289,
-0.0695301815867424,
0.017983054742217064,
-0.024090595543384552,
-0.023944292217493057,
-0.008201081305742264,
0.0165106151252985,
-0.02916831709444523,
0.008753501810133457,
0.048150867223739624,
0.03597480431199074,
-0.014900147914886475,
-0.015465314500033855,
-0.012432167306542397,
0.10410323739051819,
-0.010137240402400494
] |
PMID:18488
|
Cimetidine, an H2-antihistamine, stimulates prolactin secretion in man.
|
Intravenous administration of cimetidine, a histamine (H2) receptor antagonist, provoked an immediate 2 to 3-fold rise in serum prolactin concentrations in 8 normal men; serum thyrotropin, growth hormone, thyroxine, and triiodothyronine were not significantly affected. Diphenhydramine, an H1-antagonist, was without effect on any of the hormones measured. The results suggest that histamine may have an important function in the regulation of prolactin secretion in man.
|
Cimetidine, an H2-antihistamine, stimulates prolactin secretion in man. Intravenous administration of cimetidine, a histamine (H2) receptor antagonist, provoked an immediate 2 to 3-fold rise in serum prolactin concentrations in 8 normal men; serum thyrotropin, growth hormone, thyroxine, and triiodothyronine were not significantly affected. Diphenhydramine, an H1-antagonist, was without effect on any of the hormones measured. The results suggest that histamine may have an important function in the regulation of prolactin secretion in man.
|
[
0.011613902635872364,
0.0220522228628397,
-0.026234818622469902,
0.06812722980976105,
-0.04001684486865997,
0.03646951913833618,
0.06843061000108719,
0.017540907487273216,
-0.06380263715982437,
-0.056532058864831924,
-0.0152084119617939,
-0.03735343739390373,
0.009384829550981522,
0.049629949033260345,
0.03154696896672249,
0.009210546500980854,
0.0020121007692068815,
0.008517907001078129,
-0.08349157124757767,
-0.04559984430670738,
-0.04045607149600983,
-0.01293990295380354,
0.01672302559018135,
-0.009439414367079735,
-0.020068414509296417,
-0.001449885661713779,
-0.03726690262556076,
0.00408636499196291,
-0.08166719973087311,
-0.17906159162521362,
0.08006620407104492,
-0.030084935948252678,
-0.0046936520375311375,
0.03600718453526497,
-0.05567376688122749,
-0.0011626064078882337,
-0.05797433480620384,
0.012544526718556881,
0.03549814969301224,
-0.0018075500847771764,
0.01727297157049179,
-0.014926674775779247,
-0.03051593527197838,
0.02738328091800213,
0.005988023709505796,
0.023871375247836113,
-0.012104138731956482,
0.020468680188059807,
0.11350488662719727,
0.04631911963224411,
0.05715082585811615,
0.012626105919480324,
-0.004981039557605982,
0.09158416092395782,
0.043561775237321854,
-0.03733477368950844,
-0.07752247899770737,
0.06891755759716034,
-0.03829514980316162,
0.07500386238098145,
-0.050710540264844894,
0.0611291266977787,
-0.1355891078710556,
0.05985609069466591,
0.029465312138199806,
0.04287507012486458,
-0.004812787286937237,
-0.034701380878686905,
0.04721267893910408,
0.06627900153398514,
-0.020143836736679077,
0.005384501069784164,
-0.014441350474953651,
0.009830478578805923,
-0.006542975548654795,
-0.046233683824539185,
-0.05461689084768295,
-0.059662479907274246,
0.04454933851957321,
0.051956236362457275,
0.000677231524605304,
0.02671133168041706,
-0.02716021053493023,
-0.020773468539118767,
0.02774360217154026,
-0.057256389409303665,
0.00378728611394763,
0.024825874716043472,
-0.012582505121827126,
-0.06516460329294205,
-0.04467202350497246,
-0.09681776165962219,
-0.022229762747883797,
-0.025792686268687248,
-0.04102155566215515,
-0.025522733107209206,
0.009988383390009403,
-0.01452652271836996,
-0.03218464553356171,
0.3312018811702728,
0.016637271270155907,
-0.0540994331240654,
-0.11360623687505722,
-0.016369206830859184,
0.02841242030262947,
-0.05053101107478142,
-0.017551394179463387,
0.018615201115608215,
-0.029629504308104515,
0.04538225382566452,
0.08785460144281387,
0.08025975525379181,
0.05477422475814819,
-0.047510694712400436,
0.02296857349574566,
-0.032276567071676254,
0.08083253353834152,
0.025808870792388916,
0.006238412577658892,
-0.0005917948437854648,
0.0031923584174364805,
0.03699792921543121,
0.05495224893093109,
0.0245757345110178,
-0.020345792174339294,
0.00895765982568264,
-0.035962335765361786,
0.11083684861660004,
0.009987429715692997,
-0.014241309836506844,
0.04467460885643959,
0.0037529869005084038,
-0.03569281846284866,
0.04542331025004387,
0.008314683102071285,
0.016435585916042328,
0.03754864260554314,
-0.015075541101396084,
0.03921711444854736,
0.04539426043629646,
-0.04407257214188576,
-0.09590994566679001,
-0.07923747599124908,
-0.11127075552940369,
-0.019976580515503883,
0.10133964568376541,
0.042959198355674744,
-0.024292239919304848,
-0.03209429234266281,
0.060758545994758606,
-0.01498873345553875,
0.030380016192793846,
-0.0122936787083745,
-0.018332473933696747,
0.029978269711136818,
-0.018998268991708755,
-0.018176447600126266,
-0.026255767792463303,
-0.007388950791209936,
0.02370087057352066,
-0.08994913846254349,
-0.029828520491719246,
-0.03791290521621704,
0.05503133684396744,
-0.019682329148054123,
-0.0445716418325901,
-0.02186070941388607,
-0.019848572090268135,
0.015433598309755325,
0.05183204263448715,
-0.030755287036299706,
0.05221541225910187,
-0.08160287141799927,
0.05715697258710861,
0.011000176891684532,
0.029940126463770866,
0.006392600480467081,
-0.03471788763999939,
0.00412847101688385,
-0.012993479147553444,
0.005368285812437534,
0.006369762122631073,
-0.0027947945054620504,
0.035694420337677,
0.02282230742275715,
0.00024106200726237148,
0.031299322843551636,
0.03432384133338928,
0.030971119180321693,
0.031096234917640686,
-0.05795539915561676,
0.04013408347964287,
-0.042428065091371536,
-0.020015999674797058,
-0.02085736021399498,
-0.01089093554764986,
-0.05856230482459068,
0.06950805336236954,
-0.054614245891571045,
-0.00750433374196291,
0.07457303255796432,
0.05509860813617706,
0.028892608359456062,
0.057715628296136856,
0.012113417498767376,
0.012598523870110512,
-0.01919379085302353,
-0.011765800416469574,
0.0651697963476181,
-0.05684129148721695,
0.01168470922857523,
0.004726669751107693,
0.01328959222882986,
-0.022300608456134796,
-0.011790976859629154,
0.07236222922801971,
0.01868848316371441,
-0.011328542605042458,
0.06393955647945404,
0.0357414111495018,
0.01831774041056633,
-0.020066967234015465,
-0.023628655821084976,
-0.28708094358444214,
0.004813946317881346,
0.0011154799722135067,
-0.007373895030468702,
0.009077594615519047,
-0.12048090994358063,
0.008586942218244076,
-0.046987563371658325,
-0.008861209265887737,
0.007226159330457449,
0.0338224321603775,
0.06626448780298233,
-0.10351481288671494,
-0.008831892162561417,
0.012359954416751862,
0.06547155976295471,
0.017062952741980553,
-0.008754497393965721,
0.05531301721930504,
0.02211160957813263,
0.013916393741965294,
0.019688434898853302,
0.015611814334988594,
-0.009831256233155727,
-0.020242227241396904,
-0.008022614754736423,
0.09208943694829941,
0.03792646899819374,
-0.03007497265934944,
-0.03318857401609421,
0.0039090607315301895,
-0.0006399358971975744,
-0.015124855563044548,
-0.09760691970586777,
0.008446409367024899,
-0.03190839663147926,
0.008362498134374619,
-0.012627038173377514,
-0.011318610049784184,
-0.0006903765024617314,
-0.053685177117586136,
0.010540487244725227,
0.017075587064027786,
-0.06970930099487305,
-0.053732167929410934,
-0.028247611597180367,
0.0027028024196624756,
0.0736425518989563,
-0.015421262010931969,
-0.006223101168870926,
0.04642477631568909,
0.04339810088276863,
0.028232166543602943,
0.03613539785146713,
0.0008641018066555262,
-0.014829923398792744,
-0.061102814972400665,
0.011699853464961052,
-0.07244660705327988,
0.030418066307902336,
-0.0010459092445671558,
0.056699834764003754,
0.015796130523085594,
0.018729958683252335,
-0.07226037979125977,
0.008221796713769436,
0.005580050405114889,
-0.0207868292927742,
0.05331731587648392,
0.029570311307907104,
0.04407620057463646,
0.13523268699645996,
-0.07501418888568878,
0.03572393208742142,
0.03577756509184837,
0.0224657841026783,
0.015049288049340248,
-0.061246030032634735,
-0.07794012874364853,
0.03133004531264305,
-0.0034298684913665056,
0.012955510057508945,
0.001453331089578569,
0.044712189584970474,
-0.011510857380926609,
-0.01808730699121952,
-0.03574346378445625,
0.004418046213686466,
0.04839898645877838,
0.015086530707776546,
0.055070020258426666,
0.07040569931268692,
-0.037166252732276917,
-0.051492542028427124,
-0.020643193274736404,
0.03818144276738167,
-0.2556403577327728,
-0.006322438828647137,
0.01784643344581127,
-0.009671946987509727,
-0.0317763015627861,
0.031799107789993286,
0.04498427361249924,
-0.07797696441411972,
-0.040479958057403564,
0.004283762536942959,
0.04673008993268013,
0.018733005970716476,
0.07296701520681381,
0.03122605010867119,
-0.03311096504330635,
-0.06062646955251694,
0.005200066138058901,
-0.020200354978442192,
-0.038775403052568436,
-0.018176069483160973,
-0.010332020930945873,
-0.038927964866161346,
0.15337376296520233,
-0.01904214546084404,
0.029172824695706367,
0.013389533385634422,
0.06411408632993698,
0.015272541902959347,
0.021037021651864052,
0.03725039213895798,
-0.04487381875514984,
0.036318421363830566,
0.05070847272872925,
0.010962273925542831,
-0.0072609251365065575,
0.008460752665996552,
-0.006756944581866264,
0.0008117494871839881,
-0.02579558454453945,
0.03817857429385185,
-0.05978371202945709,
0.011020919308066368,
-0.02490980736911297,
-0.03730091080069542,
0.07116735726594925,
-0.041697677224874496,
0.018559513613581657,
-0.024566086009144783,
0.02258596569299698,
0.028623122721910477,
-0.041124287992715836,
-0.08702518790960312,
0.0445258654654026,
0.05460943654179573,
-0.004041366744786501,
0.008075270801782608,
-0.0006845697062090039,
-0.07253257185220718,
-0.03249688073992729,
-0.008793673478066921,
-0.02085130102932453,
-0.01819274201989174,
-0.044871736317873,
0.011059197597205639,
0.07577019929885864
] |
PMID:18489
|
Value of sputum culture in diagnosis of pneumococcal pneumonia.
|
In our laboratory, culture of sputum was extremely useful in diagnosing the etiology of pneumococcal pneumonia. Of 31 consecutive patients with bacteremic pneumococcal pneumonia, 29 (94%) had Streptococcus pneumoniae cultured from sputum. Recovery of pneumococci in culture was enhanced by anaerobic incubation as well as by a plate bile test and an optochin disk on a primary blood agar plate.
|
Value of sputum culture in diagnosis of pneumococcal pneumonia. In our laboratory, culture of sputum was extremely useful in diagnosing the etiology of pneumococcal pneumonia. Of 31 consecutive patients with bacteremic pneumococcal pneumonia, 29 (94%) had Streptococcus pneumoniae cultured from sputum. Recovery of pneumococci in culture was enhanced by anaerobic incubation as well as by a plate bile test and an optochin disk on a primary blood agar plate.
|
[
0.01794358715415001,
0.06204020231962204,
-0.045165032148361206,
-0.03638593107461929,
0.08325891941785812,
0.006991378031671047,
-0.03196030110120773,
0.08861616253852844,
-0.019334379583597183,
0.03318610042333603,
-0.01512373611330986,
-0.06583881378173828,
-0.023685188964009285,
0.003473985241726041,
-0.08197996020317078,
-0.041520390659570694,
-0.04322732239961624,
0.010291162878274918,
-0.07813755422830582,
-0.011414860375225544,
-0.05676080286502838,
0.03200026974081993,
-0.0315338671207428,
-0.0623336061835289,
0.053888000547885895,
-0.015962954610586166,
0.02262570708990097,
-0.058652475476264954,
-0.006545967888087034,
-0.1079828217625618,
-0.021142303943634033,
-0.0161631777882576,
0.03205089271068573,
0.0393279530107975,
-0.032402388751506805,
-0.008900374174118042,
0.03537382557988167,
-0.07478541135787964,
0.017635181546211243,
0.09678254276514053,
0.01762225851416588,
-0.00727760000154376,
0.04199917986989021,
0.0019240338588133454,
0.0174019206315279,
0.008033518679440022,
-0.006884987000375986,
0.014746801927685738,
0.12577557563781738,
0.00017843168461695313,
0.04234825819730759,
-0.03276319056749344,
-0.03791249170899391,
0.0416426807641983,
-0.09718229621648788,
-0.038677021861076355,
-0.011002308689057827,
0.029880927875638008,
-0.03586843982338905,
-0.007144242525100708,
-0.026833849027752876,
0.006914603523910046,
-0.08035977184772491,
0.025732487440109253,
-0.028814679011702538,
-0.010292064398527145,
-0.020605212077498436,
-0.051876500248909,
-0.003202019026502967,
0.03161650896072388,
-0.03892558068037033,
0.03776049613952637,
0.014839230105280876,
0.059726763516664505,
-0.008294817060232162,
0.01833909936249256,
-0.00900267343968153,
-0.03931858390569687,
0.035074349492788315,
0.043373409658670425,
-0.003781664650887251,
0.031885117292404175,
-0.010322182439267635,
0.03999542072415352,
-0.05368315428495407,
-0.04085233435034752,
0.009102795273065567,
-0.010498995892703533,
-0.04048189893364906,
0.03937733173370361,
0.014919986948370934,
0.03335631638765335,
-0.06468972563743591,
0.094056136906147,
-0.035686030983924866,
0.023552268743515015,
0.008040017448365688,
0.023541226983070374,
0.0011491068871691823,
0.3735326826572418,
-0.07017729431390762,
-0.07841238379478455,
-0.03651183843612671,
-0.02328786626458168,
-0.005958410911262035,
-0.010999087244272232,
0.012587202712893486,
-0.01596403680741787,
-0.020598994567990303,
0.021230513229966164,
-0.040047697722911835,
0.06393514573574066,
-0.0009760615648701787,
-0.01830027997493744,
-0.024923045188188553,
0.01318091806024313,
0.0786939263343811,
0.00920873787254095,
-0.08009389787912369,
0.05318008363246918,
0.0000735885914764367,
-0.01989557407796383,
0.059526730328798294,
-0.08826912939548492,
-0.00048685085494071245,
-0.008413833566009998,
0.0152316614985466,
0.07488010078668594,
0.025796223431825638,
0.005171694792807102,
0.044097933918237686,
-0.01689113676548004,
0.04221726208925247,
0.015032289549708366,
0.05779498443007469,
-0.0778048112988472,
0.013286708854138851,
-0.03150603547692299,
0.02463836409151554,
0.0649230033159256,
-0.0011383965611457825,
-0.028804028406739235,
-0.03923450782895088,
-0.05853453651070595,
-0.09459259361028671,
0.08805705606937408,
-0.07647276669740677,
0.043230921030044556,
-0.012741900980472565,
-0.11475693434476852,
0.0618458166718483,
0.05633266642689705,
0.010608362033963203,
-0.050491705536842346,
0.08168426156044006,
0.01360592432320118,
0.006086216773837805,
0.04159483313560486,
-0.014916809275746346,
0.009846700355410576,
-0.04825756326317787,
-0.04555319622159004,
0.015213225968182087,
0.038638606667518616,
-0.005261889658868313,
0.0012942332541570067,
-0.038459956645965576,
-0.04534915089607239,
-0.011362630873918533,
-0.0321536511182785,
-0.019254935905337334,
0.024441491812467575,
-0.04186049476265907,
0.009205866605043411,
0.010286829434335232,
0.045072752982378006,
-0.024322345852851868,
-0.01766618900001049,
0.018728867173194885,
0.04289626330137253,
-0.0017548620235174894,
0.04674900695681572,
0.05192863568663597,
0.03083913028240204,
-0.006404869258403778,
0.0017913610208779573,
-0.06218414008617401,
-0.01035319920629263,
0.043662842363119125,
0.016154395416378975,
0.0094253895804286,
-0.005480494815856218,
-0.034196775406599045,
-0.04633117467164993,
0.06353297829627991,
-0.03642166033387184,
-0.044613491743803024,
-0.0021224787924438715,
-0.003096572821959853,
-0.022314582020044327,
0.024534931406378746,
-0.008841277100145817,
0.02305133268237114,
0.0029647033661603928,
0.04624773934483528,
0.03755291551351547,
0.04653036966919899,
0.04118991270661354,
-0.013001466169953346,
0.03382492810487747,
-0.061255764216184616,
0.04166668280959129,
-0.051752105355262756,
0.021238505840301514,
-0.08588650822639465,
0.00024850378395058215,
0.009693319909274578,
0.005852507892996073,
-0.011825231835246086,
-0.03238938748836517,
-0.01163516752421856,
0.029466675594449043,
0.011164183728396893,
-0.30528873205184937,
0.07447357475757599,
0.05110345035791397,
0.003952178172767162,
0.04745456576347351,
0.007997236214578152,
-0.013074160553514957,
0.03391338139772415,
0.04092869162559509,
-0.04997070133686066,
0.06380509585142136,
0.03375498950481415,
-0.04754839092493057,
-0.03437883406877518,
0.018290231004357338,
0.0637468472123146,
-0.013806364499032497,
-0.05471412092447281,
-0.01831762306392193,
-0.020508376881480217,
0.029027335345745087,
-0.04202361777424812,
0.030016541481018066,
0.002920544473454356,
0.02436969242990017,
-0.06399651616811752,
0.07364240288734436,
-0.04001285880804062,
0.054005954414606094,
-0.02733512409031391,
0.021665377542376518,
0.003922225441783667,
-0.0015958264702931046,
-0.06986884027719498,
-0.037079207599163055,
0.012887435033917427,
0.002323612105101347,
0.021162286400794983,
0.006291241385042667,
0.04154811054468155,
0.004069711547344923,
-0.01868976093828678,
-0.02174769900739193,
-0.07886712998151779,
-0.013420467264950275,
-0.029963653534650803,
0.05146375298500061,
0.00939316488802433,
-0.024309005588293076,
0.041929058730602264,
-0.016829967498779297,
0.0070821261033415794,
-0.005252388771623373,
0.06899461895227432,
-0.017191046848893166,
0.00407251575961709,
0.025059480220079422,
-0.046501997858285904,
-0.0633712038397789,
-0.0446646623313427,
-0.05635449290275574,
-0.004174407105892897,
0.06766970455646515,
-0.02699105441570282,
0.026934774592518806,
-0.008948146365582943,
-0.017645973712205887,
-0.0455743744969368,
0.0724349245429039,
0.05264808237552643,
0.028599515557289124,
0.06069721281528473,
-0.03941696509718895,
-0.01620141975581646,
0.04454302415251732,
0.07411576807498932,
0.023586301133036613,
-0.053463414311409,
-0.06072347238659859,
0.006335491314530373,
0.08409161120653152,
-0.09650083631277084,
0.00039839965756982565,
0.013370073400437832,
0.07197991013526917,
0.07235044986009598,
0.0015375971561297774,
0.007891510613262653,
-0.060512665659189224,
-0.03989657387137413,
-0.01988808438181877,
0.022951755672693253,
-0.018703026697039604,
-0.062006283551454544,
-0.058788493275642395,
0.016425231471657753,
-0.2514941394329071,
0.06167177855968475,
0.005769297946244478,
0.04421743378043175,
-0.0034665153361856937,
0.028367960825562477,
-0.01954049803316593,
-0.01674058847129345,
0.035896990448236465,
0.02986247092485428,
0.13123729825019836,
-0.038074739277362823,
0.03844471648335457,
-0.027398061007261276,
-0.018849018961191177,
0.03830602020025253,
0.06328847259283066,
-0.03248823434114456,
0.10809031128883362,
-0.006823432631790638,
0.0074112326838076115,
-0.021749278530478477,
0.08678998798131943,
0.008584964089095592,
0.043177489191293716,
-0.015459123067557812,
0.05714241415262222,
0.07876143604516983,
-0.035738904029130936,
0.05808236077427864,
0.08659770339727402,
-0.054698601365089417,
0.02972072921693325,
-0.014893925748765469,
0.0074583967216312885,
-0.010802183300256729,
0.008054331876337528,
-0.059744469821453094,
-0.021192530170083046,
-0.005707177799195051,
-0.011608726345002651,
-0.004632958211004734,
-0.08186458796262741,
-0.009085509926080704,
0.05248573049902916,
-0.014310945756733418,
0.0060292440466582775,
-0.11431755870580673,
0.021330542862415314,
-0.01729629933834076,
-0.014655102044343948,
-0.019558310508728027,
0.05076298117637634,
0.04530114680528641,
0.018229061737656593,
0.044993285089731216,
-0.03039064258337021,
-0.03199906647205353,
0.03905931115150452,
-0.026715291664004326,
0.015148205682635307,
-0.06289646774530411,
-0.019494246691465378,
0.1030501127243042,
-0.02572178654372692
] |
PMID:18490
|
Cytochemical reactions of normal and neoplastic lymphocytes.
|
Rosetting and non-rosetting lymphocytes collected from normal individuals were stained for the presence of beta-glucuronidase, periodic-acid Schiff activity, gamma glutamyl transpeptidase, acid phosphatase, and alpha-naphthyl butyrate esterase. Lymphocytes which formed rosettes with sheep erythrocytes and non-rosette forming lymphocytes contained cytochemical reaction products for all five stains. Beta-glucuronidase (P less than 0-02) and acid phosphatase (P less than 0-01) were more frequently found in rosette forming lymphocytes. However, non-rosetting cells were more frequently periodic-acid Schiff positive (P less than 0-001). Gamma-glutamyl transpeptidase and alpha-naphthyl butyrate esterase were present equally in rosette and non-rosette forming lymphocytes. In addition, 33 non-Hodgkin's lymphomas were studied for cell surface markers and cytochemical reactions. In 17 of 19 B cell lymphomas, there was a paucity of lymphocytes containing beta-glucuronidase. However, in three of four T cell proliferations, there were numerous lymphoid cells positive for beta-glucuronidase. The periodic-acid Schiff and acid phosphatase reactions varied greatly within B, T, and null cell lymphomas and thus were of little diagnostic value in determining the cell of origin of these neoplastic lymphoid cells.
|
Cytochemical reactions of normal and neoplastic lymphocytes. Rosetting and non-rosetting lymphocytes collected from normal individuals were stained for the presence of beta-glucuronidase, periodic-acid Schiff activity, gamma glutamyl transpeptidase, acid phosphatase, and alpha-naphthyl butyrate esterase. Lymphocytes which formed rosettes with sheep erythrocytes and non-rosette forming lymphocytes contained cytochemical reaction products for all five stains. Beta-glucuronidase (P less than 0-02) and acid phosphatase (P less than 0-01) were more frequently found in rosette forming lymphocytes. However, non-rosetting cells were more frequently periodic-acid Schiff positive (P less than 0-001). Gamma-glutamyl transpeptidase and alpha-naphthyl butyrate esterase were present equally in rosette and non-rosette forming lymphocytes. In addition, 33 non-Hodgkin's lymphomas were studied for cell surface markers and cytochemical reactions. In 17 of 19 B cell lymphomas, there was a paucity of lymphocytes containing beta-glucuronidase. However, in three of four T cell proliferations, there were numerous lymphoid cells positive for beta-glucuronidase. The periodic-acid Schiff and acid phosphatase reactions varied greatly within B, T, and null cell lymphomas and thus were of little diagnostic value in determining the cell of origin of these neoplastic lymphoid cells.
|
[
-0.021239077672362328,
0.01078092958778143,
-0.04750385507941246,
0.05926600098609924,
0.041549477726221085,
-0.050810400396585464,
0.08180273324251175,
0.040248919278383255,
-0.01199046615511179,
-0.04897470772266388,
-0.004241711925715208,
-0.033425189554691315,
-0.008241111412644386,
0.00927161704748869,
-0.02218628115952015,
-0.029289092868566513,
0.008507777936756611,
-0.014510728418827057,
-0.056461673229932785,
-0.022852107882499695,
-0.043304383754730225,
-0.008967078290879726,
0.027865571901202202,
-0.0027423768769949675,
0.02590850181877613,
0.007543622050434351,
0.009721781127154827,
0.029595715925097466,
-0.060458213090896606,
-0.14332294464111328,
0.02585640549659729,
0.026633353903889656,
-0.053659819066524506,
-0.03316111117601395,
0.00898459367454052,
-0.0055117132142186165,
0.03359895944595337,
-0.04302496835589409,
0.00968087650835514,
0.000677261792588979,
0.0067231301218271255,
-0.026078563183546066,
-0.00744651211425662,
0.033775694668293,
0.011956837028265,
-0.01241516973823309,
-0.10220694541931152,
-0.0313444510102272,
0.04921257495880127,
0.01929290033876896,
-0.010669856332242489,
-0.02143736369907856,
0.011387689039111137,
0.03790391609072685,
-0.06275803595781326,
-0.0067822434939444065,
0.025952499359846115,
0.02684134989976883,
-0.027708515524864197,
0.015590373426675797,
-0.024297349154949188,
0.0036527218762785196,
-0.10530798137187958,
0.06049028038978577,
0.050164155662059784,
-0.0718214213848114,
-0.019038531929254532,
-0.054393868893384933,
0.048942431807518005,
0.05037550628185272,
-0.0739903599023819,
0.026235008612275124,
0.0026331741828471422,
0.04901491478085518,
0.03051120415329933,
-0.018161429092288017,
-0.0028907579835504293,
0.0049000149592757225,
0.01564760133624077,
0.0594915896654129,
0.020236067473888397,
0.0418962724506855,
0.048626404255628586,
0.008712748996913433,
0.014593275263905525,
-0.019263029098510742,
-0.05110825598239899,
-0.004611233714967966,
0.02083497680723667,
-0.018303275108337402,
-0.01899149641394615,
-0.061119403690099716,
-0.05801534280180931,
0.004320746753364801,
-0.057062581181526184,
-0.010434823110699654,
0.05848009139299393,
0.010901560075581074,
0.050295863300561905,
0.41239863634109497,
-0.06119752675294876,
0.04761093109846115,
-0.04048404470086098,
0.0028741599526256323,
-0.010846858844161034,
-0.05693671852350235,
0.01214790903031826,
-0.03886789456009865,
-0.009884310886263847,
0.03772700950503349,
-0.007708438206464052,
-0.043614186346530914,
0.022665461525321007,
-0.06062883511185646,
-0.018864711746573448,
-0.030238881707191467,
0.038375288248062134,
-0.03760760650038719,
-0.07590823620557785,
0.05651595816016197,
0.0025361201260238886,
-0.027585744857788086,
-0.016767270863056183,
-0.0469176359474659,
0.060424551367759705,
0.036774080246686935,
0.01363581046462059,
0.0632365345954895,
0.08219148218631744,
0.003233651863411069,
0.07074887305498123,
0.04549577459692955,
-0.017729612067341805,
0.03740188106894493,
-0.018055880442261696,
0.013758278451859951,
0.026396717876195908,
0.013486690819263458,
0.032293349504470825,
-0.023744599893689156,
0.011761460453271866,
-0.0674881860613823,
-0.04437938332557678,
-0.07629287987947464,
-0.007546500768512487,
0.08632410317659378,
-0.01888458803296089,
0.022962694987654686,
-0.005043026991188526,
0.012380758300423622,
-0.0033114252146333456,
0.06035708263516426,
0.013628661632537842,
0.03761958330869675,
-0.014076272957026958,
-0.018532216548919678,
-0.011744188144803047,
-0.009631454013288021,
0.02034085988998413,
0.0021070437505841255,
-0.013844975270330906,
0.004632250405848026,
0.01597929373383522,
0.05924065038561821,
-0.011897106654942036,
-0.026730326935648918,
-0.026407785713672638,
-0.04210333526134491,
0.013372101821005344,
0.0008192371460609138,
0.02438008412718773,
0.0623866505920887,
-0.03607844561338425,
-0.03391994908452034,
-0.0333712063729763,
0.03691864013671875,
-0.002139991382136941,
-0.002053990727290511,
0.06438383460044861,
0.03660791739821434,
0.04501169174909592,
0.018195556476712227,
-0.0530368909239769,
0.04443308338522911,
0.022977925837039948,
-0.04202590137720108,
-0.02687792293727398,
0.010775677859783173,
0.03689659386873245,
0.06159185618162155,
-0.030908480286598206,
-0.03322404995560646,
-0.09008168429136276,
-0.03114328160881996,
-0.019612716510891914,
-0.05420956015586853,
-0.04152365028858185,
-0.040413662791252136,
0.046735506504774094,
-0.007432979065924883,
0.07094214111566544,
-0.002786876866593957,
0.02271169051527977,
0.0344766229391098,
0.04955281689763069,
0.026731865480542183,
0.02178824692964554,
0.017092589288949966,
0.06052958965301514,
-0.013630235567688942,
-0.02370951883494854,
0.01032454613596201,
-0.0470082052052021,
0.015468130819499493,
-0.07186206430196762,
0.053737953305244446,
-0.06573174148797989,
-0.00187287840526551,
0.039181023836135864,
-0.040028270334005356,
0.03402220457792282,
-0.0347633957862854,
-0.08060261607170105,
-0.26577556133270264,
-0.03877950459718704,
0.027908703312277794,
-0.029752161353826523,
-0.027599068358540535,
0.023961380124092102,
-0.02003645896911621,
-0.01478156354278326,
0.009620900265872478,
0.007604734972119331,
0.08523370325565338,
0.08765129745006561,
-0.046814851462841034,
-0.13434998691082,
0.03174916282296181,
0.038451094180345535,
0.0027433279901742935,
-0.03977634757757187,
0.05690216273069382,
0.018399950116872787,
-0.0020525704603642225,
-0.05309675633907318,
0.00876674149185419,
0.03318419307470322,
0.04338787868618965,
-0.03849196061491966,
0.1445242166519165,
0.03528381511569023,
0.006436048541218042,
-0.027038926258683205,
0.02406254969537258,
0.037958066910505295,
-0.02553541399538517,
-0.003545190906152129,
0.06587286293506622,
-0.04929706081748009,
-0.047019485384225845,
0.005354064982384443,
-0.04201300069689751,
0.03847985714673996,
-0.005374853499233723,
-0.0340469554066658,
-0.03978181257843971,
-0.08032388985157013,
0.016454311087727547,
0.006634462159126997,
0.041620951145887375,
-0.01054917462170124,
0.007205555215477943,
-0.014702282845973969,
0.027885599061846733,
-0.006567009259015322,
0.048136625438928604,
-0.012022892013192177,
-0.004492772743105888,
-0.035917893052101135,
-0.05213029310107231,
0.015372920781373978,
-0.08130163699388504,
-0.01807456836104393,
0.010461193509399891,
0.00010951793228741735,
-0.009355620481073856,
0.0001294358226004988,
0.042213499546051025,
-0.00040960151818580925,
-0.006243423093110323,
-0.038041774183511734,
0.07131136208772659,
0.01927093230187893,
-0.015326658263802528,
0.12580697238445282,
-0.0027485748287290335,
0.006617865990847349,
-0.008587237447500229,
0.0266231931746006,
-0.03162304311990738,
-0.033183638006448746,
-0.05832373723387718,
0.01906553842127323,
0.0028705052100121975,
-0.06460192799568176,
0.09877258539199829,
-0.010202039033174515,
-0.012876059859991074,
0.08310668915510178,
0.04593810439109802,
-0.031898051500320435,
0.01667606271803379,
0.01342427171766758,
0.032093241810798645,
0.02147735469043255,
-0.02239115908741951,
-0.021045006811618805,
-0.023323815315961838,
-0.0068662757985293865,
-0.28993716835975647,
0.04520023614168167,
0.004397903569042683,
-0.0026708105579018593,
-0.05335761234164238,
-0.0007794817793183029,
0.021173525601625443,
-0.018159044906497,
-0.03828031197190285,
0.06157795339822769,
0.04957186430692673,
0.030701184645295143,
0.06923463940620422,
-0.00041469233110547066,
-0.06412983685731888,
0.007906937971711159,
0.011886164546012878,
-0.0031042555347085,
0.0027899194974452257,
0.0005390036385506392,
0.006598536390811205,
-0.04093046113848686,
0.17189253866672516,
0.07333777099847794,
0.014864681288599968,
0.06624055653810501,
-0.009256869554519653,
0.006457029841840267,
-0.07189495116472244,
0.022008150815963745,
0.019813986495137215,
0.04655446112155914,
0.05221327021718025,
-0.031716082245111465,
-0.030775900930166245,
0.04261264577507973,
-0.013129329308867455,
0.03312022238969803,
-0.06443513929843903,
0.03456118702888489,
-0.01788954623043537,
0.09316173195838928,
-0.08174170553684235,
-0.016193607822060585,
0.07099811732769012,
-0.07562941312789917,
-0.058716464787721634,
-0.07515624165534973,
0.01636483706533909,
-0.014291176572442055,
-0.045189984142780304,
-0.03430160880088806,
0.0043854075483977795,
0.01655801571905613,
0.003267925698310137,
-0.022289715707302094,
-0.012857943773269653,
0.04269322752952576,
0.017686666920781136,
0.03652842715382576,
0.04509269446134567,
-0.007633888628333807,
-0.022043665871024132,
0.1052495688199997,
0.0024718656204640865
] |
PMID:18491
|
The effect of a new benzodiazepine on the polygraphically monitored sleep of normal volunteers.
|
All-night sleep was polygraphically monitored from ten normal volunteers who took placebo and three dosage levels of a new benzodiazepine hypnotic, SCH 16134, in a double-blind, crossover design. All dosages of the drug decreased the time to fall asleep, and the two highest dosages also decreased interspersed wakefulness. REM sleep was suppressed, but slow-wave sleep was not affected in this experiment. The subjective quality of sleep was improved by the new hypnotic. One subject reported that he felt lethargic the day after the largest dose.
|
The effect of a new benzodiazepine on the polygraphically monitored sleep of normal volunteers. All-night sleep was polygraphically monitored from ten normal volunteers who took placebo and three dosage levels of a new benzodiazepine hypnotic, SCH 16134, in a double-blind, crossover design. All dosages of the drug decreased the time to fall asleep, and the two highest dosages also decreased interspersed wakefulness. REM sleep was suppressed, but slow-wave sleep was not affected in this experiment. The subjective quality of sleep was improved by the new hypnotic. One subject reported that he felt lethargic the day after the largest dose.
|
[
-0.026392219588160515,
0.03925595432519913,
-0.0075874910689890385,
0.058154743164777756,
0.021731816232204437,
0.01946711353957653,
0.009998288936913013,
0.00787344016134739,
-0.006292619276791811,
-0.015873435884714127,
0.008439712226390839,
0.046266913414001465,
0.013192601501941681,
0.011270751245319843,
0.0015957762952893972,
0.0004974300973117352,
0.03937657177448273,
-0.09243231266736984,
-0.03541814163327217,
0.010117197409272194,
-0.03596009686589241,
-0.022832809016108513,
0.08071109652519226,
0.03964414447546005,
0.023297512903809547,
0.025702400133013725,
0.026614798232913017,
-0.040434397757053375,
-0.05719984322786331,
-0.18909946084022522,
0.024142704904079437,
-0.007822326384484768,
0.03490005061030388,
-0.022151941433548927,
-0.013994704931974411,
0.004723002668470144,
0.011476555839180946,
0.07722658663988113,
-0.014007960446178913,
0.030757103115320206,
0.06073019281029701,
0.05652149021625519,
-0.01580536738038063,
-0.021790990605950356,
0.045712389051914215,
-0.005740976892411709,
-0.011324283666908741,
-0.03379065915942192,
0.059671878814697266,
0.028106655925512314,
0.03949495777487755,
-0.02971876598894596,
0.034344445914030075,
0.052676890045404434,
-0.014322655275464058,
-0.009672573767602444,
-0.019643720239400864,
0.0030858430545777082,
0.0005076624220237136,
0.06874430924654007,
-0.005576999392360449,
0.04891367629170418,
-0.13616545498371124,
-0.019054802134633064,
0.05054936930537224,
0.014734852127730846,
-0.04552663117647171,
-0.04114476591348648,
0.05624004825949669,
0.05877143517136574,
-0.09758153557777405,
-0.014649005606770515,
-0.033950209617614746,
0.021523883566260338,
0.042881522327661514,
-0.02827472798526287,
0.05661248788237572,
-0.004231628030538559,
-0.05245353654026985,
-0.06491692364215851,
0.005948145408183336,
-0.028621835634112358,
0.03328852355480194,
-0.055790770798921585,
0.0372515507042408,
-0.044878121465444565,
0.05188839137554169,
0.04855363816022873,
0.02461688034236431,
-0.044297315180301666,
0.029481761157512665,
-0.015697579830884933,
-0.003585953963920474,
-0.061655592173337936,
-0.005862018093466759,
-0.0015215538442134857,
-0.011433052830398083,
0.033638253808021545,
0.0010860527399927378,
0.39296191930770874,
0.02999606542289257,
0.027916714549064636,
-0.03772761672735214,
0.018920058384537697,
-0.012887587770819664,
-0.046382274478673935,
0.010739914141595364,
0.023126808926463127,
0.006989607587456703,
0.034666694700717926,
0.015417673625051975,
-0.01949463225901127,
0.1047496497631073,
0.026595303788781166,
0.0023094145581126213,
0.0172621700912714,
0.041296567767858505,
0.018721815198659897,
0.060581907629966736,
0.03615525737404823,
-0.018135305494070053,
-0.013498423621058464,
0.04496315121650696,
0.006344176363199949,
0.08718749135732651,
0.00867696013301611,
-0.014380624517798424,
0.0754779726266861,
-0.00021686918626073748,
-0.029768239706754684,
0.009472540579736233,
-0.037909135222435,
0.031913526356220245,
0.03735903650522232,
0.0027161999605596066,
0.0013721684226766229,
0.004068485461175442,
-0.04130285978317261,
0.010888434015214443,
-0.033034808933734894,
0.025487350299954414,
-0.00561533123254776,
0.019262010231614113,
-0.0843835398554802,
-0.06786283850669861,
0.10207507014274597,
-0.000720315845683217,
0.028060628101229668,
-0.05773000419139862,
0.020230820402503014,
0.0074614002369344234,
0.009257049299776554,
-0.03429592400789261,
0.017024025321006775,
0.005176846869289875,
0.04013272747397423,
0.019489577040076256,
0.02141992747783661,
-0.04841716215014458,
0.0056432392448186874,
-0.02133631892502308,
-0.029123634099960327,
0.03714388981461525,
0.03460276499390602,
-0.017510361969470978,
0.0039405664429068565,
-0.009934669360518456,
-0.036473654210567474,
-0.015035155229270458,
-0.028632663190364838,
-0.017375148832798004,
0.026229187846183777,
-0.03303694725036621,
0.004598900210112333,
-0.0036018944811075926,
-0.02040357142686844,
0.03503330796957016,
-0.05117795988917351,
0.005563682410866022,
-0.021650109440088272,
0.00006166883395053446,
-0.014233923517167568,
-0.07038635015487671,
0.04845557361841202,
-0.03838587924838066,
0.01911359466612339,
-0.04307572916150093,
0.06193561851978302,
0.007413279265165329,
0.025250699371099472,
-0.02808411605656147,
-0.03494475781917572,
-0.05126269906759262,
0.0026291378308087587,
-0.012951659969985485,
-0.012400506995618343,
-0.08021298050880432,
0.022720009088516235,
-0.019629500806331635,
0.009370739571750164,
0.08659849315881729,
0.05551939830183983,
0.03552716225385666,
0.03336269035935402,
0.07381941378116608,
0.0924212783575058,
0.012073001824319363,
-0.02542964741587639,
0.09821509569883347,
-0.0026280744932591915,
-0.002828660188242793,
-0.01819114200770855,
0.022492779418826103,
0.012563560158014297,
-0.028567682951688766,
0.06739810854196548,
-0.043541837483644485,
0.011274795047938824,
-0.019937071949243546,
0.04146261140704155,
0.024675795808434486,
-0.05094342306256294,
-0.02607978880405426,
-0.3224439024925232,
-0.01990303210914135,
0.014174550771713257,
-0.01327904686331749,
0.04119601473212242,
-0.029512440785765648,
-0.013988696038722992,
-0.008136318996548653,
-0.024117054417729378,
-0.061660755425691605,
0.012523574754595757,
0.040956661105155945,
-0.08303377777338028,
-0.01056988537311554,
0.006224159616976976,
0.031751248985528946,
0.033893734216690063,
-0.01963934116065502,
-0.04020747169852257,
-0.09351003170013428,
-0.01569868065416813,
0.020248867571353912,
0.012426451779901981,
0.01855645701289177,
0.021757248789072037,
-0.02437831088900566,
0.07331763207912445,
-0.043544951826334,
0.03235755115747452,
0.010730164125561714,
0.011531975120306015,
0.07462498545646667,
0.024987543001770973,
-0.11584852635860443,
0.020503288134932518,
0.005198275204747915,
0.050447266548871994,
0.024410545825958252,
-0.018413761630654335,
-0.04548221081495285,
-0.07768208533525467,
0.024241004139184952,
0.029158540070056915,
-0.07243581861257553,
-0.10355124622583389,
-0.020987439900636673,
0.009283077903091908,
-0.040460292249917984,
-0.04641228914260864,
-0.004172297660261393,
-0.01248613279312849,
0.0006032700184732676,
0.010979145765304565,
-0.0341431125998497,
0.059264954179525375,
-0.06538178026676178,
-0.11082659661769867,
-0.04794049263000488,
-0.0499434694647789,
-0.01577015034854412,
0.03076246567070484,
0.0472317673265934,
0.05039531737565994,
-0.04242287203669548,
-0.044839296489953995,
0.009116980247199535,
-0.024650199338793755,
-0.04385189339518547,
0.04991072416305542,
0.03680747374892235,
-0.055819932371377945,
0.10187917202711105,
-0.06235433742403984,
-0.03605218604207039,
0.0705874040722847,
0.010082298889756203,
-0.036211784929037094,
-0.01569845899939537,
-0.09898082911968231,
-0.01007678173482418,
-0.016375895589590073,
-0.007963460870087147,
0.008451051078736782,
0.024602467194199562,
-0.038671765476465225,
-0.009876104071736336,
0.00809019710868597,
0.028024863451719284,
-0.007630322128534317,
0.04859406128525734,
0.004031123127788305,
-0.02968171052634716,
0.0165083184838295,
-0.06505979597568512,
0.030069056898355484,
0.009598414413630962,
-0.23021620512008667,
-0.016832266002893448,
-0.03519737347960472,
0.02995428442955017,
0.005015076603740454,
0.019662819802761078,
0.022476578131318092,
0.008522817865014076,
-0.052201103419065475,
-0.04355062544345856,
-0.05253990739583969,
0.024129509925842285,
-0.011341217905282974,
0.07884020358324051,
-0.03232037276029587,
0.01783556304872036,
0.02241220511496067,
-0.026248129084706306,
0.04164960980415344,
0.045284923166036606,
-0.044381823390722275,
0.07900915294885635,
0.10341929644346237,
0.027117770165205002,
0.013156119734048843,
0.04682027921080589,
0.02890251763164997,
0.018671171739697456,
-0.017668042331933975,
0.1042499765753746,
-0.030364783480763435,
0.0022257145028561354,
0.09119391441345215,
-0.025034114718437195,
0.017818043008446693,
-0.023177478462457657,
-0.08525301516056061,
-0.02179918810725212,
0.02729695662856102,
-0.009387084282934666,
0.006452979054301977,
-0.0017208814388141036,
0.0032552173361182213,
-0.04608262702822685,
0.1335623860359192,
-0.026682239025831223,
-0.00935774203389883,
-0.07841522991657257,
-0.019116783514618874,
-0.007904539816081524,
-0.03980059176683426,
-0.07408229261636734,
-0.013314622454345226,
0.06438083201646805,
0.02492004632949829,
0.06621022522449493,
-0.011168337427079678,
0.020673083141446114,
0.05494010075926781,
-0.023968955501914024,
-0.0548480786383152,
-0.06127377972006798,
-0.013348855078220367,
0.03762523829936981,
-0.018431315198540688
] |
PMID:18492
|
Multiclinic double-blind comparison of triazolam and flurazepam for seven nights in outpatients with insomnia.
|
In this two-clinic seven-day double-blind study, 0.5 mg triazolam (Halcion) was compared to flurazepam (Dalmane) in the treatment of insomnia. Two clinical investigators completed 118 outpatients, 61 on triazolam and 57 on flurazepam. Five patients, four on triazolam and one on flurazepam, discontinued because of side effects; and three patients, one on triazolam and two on flurazepam, discontinued because of ineffectiveness of the medication. Analysis of pooled data for the 110 evaluable patients showed that 0.5 mg triazolam was significantly better than 30 mg flurazepam on the following parameters: (1) how much the medication helped the patients sleep, (2) onset of sleep, (3) duration of sleep, (4) evaluation of duration of sleep, and (5) feeling of restfulness in the morning. The trend for all other parameters favored triazolam treatment, but the values did not reach statistical significance. Side effects were similar in both groups, with drowsiness being reported most frequently. No change in efficacy indicating tolerance development during the seven days of drug administration was observed in either group.
|
Multiclinic double-blind comparison of triazolam and flurazepam for seven nights in outpatients with insomnia. In this two-clinic seven-day double-blind study, 0.5 mg triazolam (Halcion) was compared to flurazepam (Dalmane) in the treatment of insomnia. Two clinical investigators completed 118 outpatients, 61 on triazolam and 57 on flurazepam. Five patients, four on triazolam and one on flurazepam, discontinued because of side effects; and three patients, one on triazolam and two on flurazepam, discontinued because of ineffectiveness of the medication. Analysis of pooled data for the 110 evaluable patients showed that 0.5 mg triazolam was significantly better than 30 mg flurazepam on the following parameters: (1) how much the medication helped the patients sleep, (2) onset of sleep, (3) duration of sleep, (4) evaluation of duration of sleep, and (5) feeling of restfulness in the morning. The trend for all other parameters favored triazolam treatment, but the values did not reach statistical significance. Side effects were similar in both groups, with drowsiness being reported most frequently. No change in efficacy indicating tolerance development during the seven days of drug administration was observed in either group.
|
[
0.009591851383447647,
-0.021849121898412704,
-0.02229446917772293,
0.07771319150924683,
0.04456144943833351,
0.0021014998201280832,
0.03179822117090225,
0.042916834354400635,
0.023280074819922447,
-0.08058174699544907,
0.0030097956769168377,
-0.0026509424205869436,
-0.020387914031744003,
-0.018265090882778168,
0.008463531732559204,
-0.01625896990299225,
0.04572214558720589,
-0.07403191924095154,
-0.05023767426609993,
-0.004131705034524202,
0.034357763826847076,
-0.03382587060332298,
0.022256938740611076,
-0.006261866074055433,
0.0034024619963020086,
0.006912797689437866,
0.008158271200954914,
-0.0037522553466260433,
-0.07458996772766113,
-0.1543133556842804,
0.0023454632610082626,
0.019392993301153183,
0.019003817811608315,
-0.04618602246046066,
-0.044515207409858704,
0.027895448729395866,
0.003926570061594248,
0.014582270756363869,
-0.04490552097558975,
0.0042490120977163315,
0.07673437893390656,
0.05198942869901657,
-0.019356543198227882,
0.0056495205499231815,
-0.04065334051847458,
-0.03678928688168526,
-0.03197769075632095,
-0.044284041970968246,
0.09766526520252228,
0.04768387973308563,
0.013719105161726475,
-0.06732933223247528,
0.03634049743413925,
0.04330069199204445,
-0.027490131556987762,
-0.003815591102465987,
-0.047140270471572876,
0.0008072613854892552,
-0.05752105638384819,
0.0305852759629488,
0.00529932277277112,
0.0325072780251503,
-0.151340514421463,
-0.010801046155393124,
0.010019904933869839,
-0.03218040615320206,
-0.021922925487160683,
-0.049864836037158966,
0.06025254353880882,
0.062187664210796356,
-0.07098758220672607,
0.001804619561880827,
-0.003258599666878581,
0.03425108268857002,
-0.004418050870299339,
-0.03396724537014961,
0.06847157329320908,
0.009348190389573574,
-0.013288334012031555,
-0.05293382331728935,
0.033120252192020416,
-0.0651591494679451,
0.01109483651816845,
-0.08607209473848343,
0.021264608949422836,
-0.004986448213458061,
0.004736118484288454,
0.028288567438721657,
-0.007786694914102554,
-0.009643003344535828,
-0.002421746961772442,
0.0006801695562899113,
-0.025729147717356682,
-0.06017763167619705,
-0.016058973968029022,
-0.010180252604186535,
-0.028017999604344368,
0.013164449483156204,
-0.030399052426218987,
0.359676718711853,
0.08913514763116837,
0.037869688123464584,
-0.01834825426340103,
-0.04619060829281807,
0.021368809044361115,
-0.019855348393321037,
0.022150926291942596,
-0.04114373028278351,
0.011007335036993027,
0.06587427109479904,
0.02662372961640358,
-0.006629389710724354,
0.07640623301267624,
-0.013970557600259781,
-0.03266695514321327,
0.03481101617217064,
0.10143454372882843,
-0.0018217400647699833,
0.07499067485332489,
0.08137334138154984,
-0.05870577692985535,
-0.05283920466899872,
0.03740306571125984,
-0.004096513148397207,
0.02477455884218216,
-0.010624293237924576,
-0.03863013908267021,
0.06120619550347328,
0.008818067610263824,
-0.015719039365649223,
0.07561050355434418,
-0.03158782050013542,
0.010356007143855095,
0.031013615429401398,
0.03879741206765175,
-0.02766905166208744,
0.0649697482585907,
-0.012212222442030907,
0.03616233915090561,
-0.0940457358956337,
0.037672437727451324,
-0.10470656305551529,
0.06792758405208588,
-0.04720665141940117,
-0.03702753782272339,
0.10207372158765793,
-0.04318859055638313,
-0.01387536246329546,
-0.061163291335105896,
0.008027026429772377,
-0.01803184300661087,
0.028029030188918114,
0.0038366636727005243,
0.027559243142604828,
0.00858635175973177,
0.0448320172727108,
0.055363189429044724,
0.03327708691358566,
-0.015445195138454437,
0.0025795013643801212,
-0.0020549502223730087,
-0.016600653529167175,
0.03800215572118759,
0.0597364716231823,
-0.040499214082956314,
0.004734598100185394,
-0.02925652451813221,
0.034597694873809814,
-0.07722872495651245,
-0.002341712825000286,
0.07091101258993149,
0.009203948080539703,
-0.01854119636118412,
-0.07412782311439514,
0.01892012171447277,
-0.01929345354437828,
0.04190709814429283,
-0.03814370557665825,
0.0014500136021524668,
-0.03313298150897026,
0.015527761541306973,
0.03912774473428726,
-0.010328816249966621,
0.019024686887860298,
-0.011327947489917278,
0.023916535079479218,
-0.017067745327949524,
0.01775660365819931,
0.022060323506593704,
0.00042153371032327414,
-0.029588881880044937,
-0.0038926110137254,
-0.054842907935380936,
0.02727370709180832,
-0.029968976974487305,
-0.054476410150527954,
-0.08323623985052109,
0.01191615778952837,
0.01976405456662178,
0.025037972256541252,
0.05995552986860275,
0.07700226455926895,
0.06218628212809563,
0.02632586658000946,
0.05617181584239006,
0.07393787801265717,
-0.03969869762659073,
-0.02152499184012413,
0.05990071967244148,
-0.003702910151332617,
0.04207295924425125,
0.04826877638697624,
0.030882908031344414,
-0.003129631746560335,
-0.023634029552340508,
0.051649194210767746,
0.0044970326125621796,
0.037979237735271454,
-0.0064297146163880825,
0.04878700524568558,
0.009724617004394531,
-0.027763964608311653,
-0.0008442633552476764,
-0.31137174367904663,
-0.04121752455830574,
0.00045443049748428166,
-0.009918281808495522,
0.04456298425793648,
-0.04631757736206055,
0.010595384053885937,
-0.04805174469947815,
-0.04649638012051582,
-0.0066401539370417595,
-0.02050807699561119,
-0.0031518617179244757,
-0.08412500470876694,
0.011103267781436443,
-0.05772792175412178,
0.05097777768969536,
0.04191725328564644,
0.03233290836215019,
0.02111070044338703,
-0.07204226404428482,
0.017040086910128593,
0.012181672267615795,
-0.03004376031458378,
0.02010192535817623,
-0.005385609343647957,
0.005675270687788725,
0.10202009975910187,
-0.046249937266111374,
0.002070463728159666,
0.040925007313489914,
0.025188442319631577,
0.0758364126086235,
0.023458730429410934,
-0.11168007552623749,
0.005666332319378853,
-0.02016606368124485,
0.039065051823854446,
-0.011721246875822544,
-0.015405484475195408,
-0.03163665905594826,
-0.03546503186225891,
0.05691084265708923,
0.04202646389603615,
-0.08032651990652084,
-0.07939767837524414,
-0.004593849182128906,
0.0106212692335248,
-0.05049418658018112,
-0.001423163223080337,
0.0061869085766375065,
-0.022952523082494736,
0.02249772846698761,
-0.002158079296350479,
-0.04480526223778725,
0.023556821048259735,
-0.051669616252183914,
-0.09609021246433258,
0.002559461398050189,
-0.06010279804468155,
-0.061894990503787994,
-0.021343063563108444,
-0.013047237880527973,
0.08177831768989563,
0.006872642785310745,
0.013311108574271202,
0.007422972470521927,
0.0020740691106766462,
-0.037514641880989075,
0.04839978739619255,
0.04995613917708397,
-0.00486023910343647,
0.10437773913145065,
-0.0659036636352539,
-0.05072217807173729,
0.03801143169403076,
0.014977850019931793,
-0.013604097999632359,
-0.01013077050447464,
-0.07024841010570526,
0.033619705587625504,
-0.006782065145671368,
-0.0015701951924711466,
0.04658522084355354,
0.04591911658644676,
-0.0038904098328202963,
-0.03992607817053795,
-0.012564455159008503,
0.00494758365675807,
-0.010511405766010284,
0.002821934875100851,
0.0400247760117054,
-0.042038824409246445,
0.038978926837444305,
-0.027910875156521797,
-0.009371436201035976,
0.00645459396764636,
-0.2491556853055954,
0.01702003739774227,
-0.043573785573244095,
0.05647436901926994,
0.016770213842391968,
-0.02778375893831253,
0.03443995863199234,
-0.009128951467573643,
-0.034716568887233734,
-0.10809974372386932,
0.001439905259758234,
0.06317605823278427,
-0.006059568841010332,
-0.006139129865914583,
0.04240124300122261,
-0.00533623993396759,
0.05791020020842552,
-0.05547375604510307,
0.029448360204696655,
0.006829352118074894,
-0.013710254803299904,
0.03481864184141159,
0.10759525001049042,
0.01291409507393837,
0.0037989541888237,
0.05905691906809807,
0.012039518915116787,
0.014211439527571201,
-0.019213033840060234,
0.06785863637924194,
-0.007234520744532347,
0.031607743352651596,
0.052949462085962296,
0.007693104445934296,
-0.0032049277797341347,
-0.011280226521193981,
-0.08723460137844086,
-0.010263247415423393,
0.03765428811311722,
0.015355154871940613,
0.011022365652024746,
0.017674628645181656,
-0.014458004385232925,
0.01304092537611723,
0.10590091347694397,
-0.10765387117862701,
0.03130009025335312,
-0.034588251262903214,
-0.023672068491578102,
-0.04468503221869469,
-0.05031076818704605,
-0.048272810876369476,
-0.00633523752912879,
0.07044106721878052,
0.03693697229027748,
0.06807024031877518,
-0.03486384078860283,
0.0022301655262708664,
0.008414764888584614,
-0.025149082764983177,
-0.09201414883136749,
0.005322530400007963,
0.062488120049238205,
0.03976127505302429,
0.051308274269104004
] |
PMID:18493
|
Effect of a new benzodiazepine derivate, clobazam, in anxious patients with gastrointestinal disorders.
|
Thirty-four anxious patients with gastrointestinal disorders were studied in order to evaluate the effectiveness of a new 1,5-benzodiazepine antianxiety agent (HR 376). The disorders were classified as organic or functional according to the presence or absence of radiologic signs of ulcer. Dietetic measures, gastric antacids, anticholinergic agents, and antianxiety treatment were applied for six weeks. Anxiolytic treatment consisted of 30 mg/day clobazam (HR 376) or 15 mg/day diazepam, given in a randomized, double-blind manner. Clinical follow-up was performed with the PEN Personality Inventory (PEN), Taylor Manifest Anxiety Scale (TMAS), Hamilton Anxiety Scale (HAS), and Wittenborn Psychiatric Rating Scales (WPRS). The score of the psychoticism dimension of the PEN inventory was significantly higher in organic than in functional patients. Significant differences occurred in the reduction of the rating scores of HAS and WPRS before/after treatment in the clobazam and diazepam groups. This would express a modification of state anxiety. The TMAS, which evaluates trait anxiety, disclosed statistically significant improvement in the clobazam group. This group showed an early reduction of the HAS and TMAS scores, which would suggest an early onset of action.
|
Effect of a new benzodiazepine derivate, clobazam, in anxious patients with gastrointestinal disorders. Thirty-four anxious patients with gastrointestinal disorders were studied in order to evaluate the effectiveness of a new 1,5-benzodiazepine antianxiety agent (HR 376). The disorders were classified as organic or functional according to the presence or absence of radiologic signs of ulcer. Dietetic measures, gastric antacids, anticholinergic agents, and antianxiety treatment were applied for six weeks. Anxiolytic treatment consisted of 30 mg/day clobazam (HR 376) or 15 mg/day diazepam, given in a randomized, double-blind manner. Clinical follow-up was performed with the PEN Personality Inventory (PEN), Taylor Manifest Anxiety Scale (TMAS), Hamilton Anxiety Scale (HAS), and Wittenborn Psychiatric Rating Scales (WPRS). The score of the psychoticism dimension of the PEN inventory was significantly higher in organic than in functional patients. Significant differences occurred in the reduction of the rating scores of HAS and WPRS before/after treatment in the clobazam and diazepam groups. This would express a modification of state anxiety. The TMAS, which evaluates trait anxiety, disclosed statistically significant improvement in the clobazam group. This group showed an early reduction of the HAS and TMAS scores, which would suggest an early onset of action.
|
[
-0.01958751119673252,
-0.010575073771178722,
0.05112931877374649,
0.07740228623151779,
-0.014297539368271828,
0.0019920039921998978,
0.06434404850006104,
0.04214691370725632,
-0.0508020855486393,
-0.08347200602293015,
0.00782767217606306,
-0.023550599813461304,
-0.00048585503827780485,
0.0019315745448693633,
-0.008553484454751015,
0.004444352816790342,
0.07758281379938126,
-0.06683683395385742,
-0.01353847049176693,
-0.0003815981326624751,
-0.04587329179048538,
-0.008060154505074024,
0.03718658164143562,
0.005740025546401739,
0.013466932810842991,
0.014540918171405792,
0.0037344847805798054,
-0.009907633066177368,
-0.09625767171382904,
-0.1489899456501007,
0.015145511366426945,
-0.04477756842970848,
0.07022199034690857,
-0.03882751986384392,
-0.03375110402703285,
0.017732148990035057,
-0.04501684010028839,
0.07812411338090897,
-0.09285610169172287,
-0.009729704819619656,
0.05176717787981033,
0.06970694661140442,
-0.015833834186196327,
-0.030270371586084366,
0.008077806793153286,
-0.04537634924054146,
0.008702280931174755,
0.004240045323967934,
0.0709293857216835,
0.030893400311470032,
-0.006236710119992495,
-0.03667259216308594,
0.01537812314927578,
0.03815650939941406,
0.005717797204852104,
0.0011348106199875474,
-0.05495641008019447,
-0.03355900198221207,
-0.06598591804504395,
0.014565556310117245,
0.04077194631099701,
0.016964402049779892,
-0.139671191573143,
0.047505080699920654,
-0.0019490589620545506,
-0.007069181650876999,
0.004237296059727669,
-0.038295142352581024,
0.0430581234395504,
0.07264380902051926,
-0.06233566254377365,
0.00010113044118043035,
0.03103497065603733,
0.049861472100019455,
0.027743978425860405,
-0.021931728348135948,
0.013730441220104694,
-0.05920834094285965,
-0.036034148186445236,
-0.007143820635974407,
-0.031885288655757904,
0.02676980011165142,
0.014458343386650085,
-0.08738935738801956,
0.019437918439507484,
-0.013002630323171616,
0.03571439906954765,
-0.014997598715126514,
0.012944938614964485,
-0.001783630345016718,
0.05149387568235397,
-0.05195888504385948,
0.034227367490530014,
-0.0644102543592453,
0.0009450062643736601,
-0.018381716683506966,
-0.015746301040053368,
-0.01439187303185463,
-0.002310537500306964,
0.41032078862190247,
0.0033251841086894274,
0.008273526094853878,
0.01644141413271427,
-0.0030662768986076117,
0.0519968643784523,
0.028419340029358864,
0.03223985806107521,
-0.04188293218612671,
-0.025529392063617706,
0.02488127537071705,
0.05678974837064743,
-0.01822633109986782,
0.0804135873913765,
-0.02265162579715252,
-0.04754623770713806,
0.034432582557201385,
0.07011085748672485,
0.003081692848354578,
0.04105893895030022,
0.06613028794527054,
-0.036965999752283096,
-0.019585279747843742,
0.04203038290143013,
0.006784404627978802,
0.048689499497413635,
-0.00504600815474987,
-0.06265097856521606,
0.0664447546005249,
-0.013052129186689854,
-0.0071788751520216465,
0.020521044731140137,
-0.03675849363207817,
0.01808355003595352,
0.04819225147366524,
0.028335150331258774,
0.03351716697216034,
0.008306438103318214,
0.020276447758078575,
-0.002808429766446352,
-0.001134085701778531,
0.030440248548984528,
-0.027777209877967834,
0.03295813873410225,
-0.09156131744384766,
-0.06361639499664307,
0.10407532751560211,
0.005146445240825415,
0.004622443579137325,
-0.05982806161046028,
-0.005739413667470217,
-0.0231503713876009,
0.03378361091017723,
-0.0017034639604389668,
0.07880005240440369,
-0.05565912276506424,
0.03977549076080322,
-0.018024900928139687,
0.03404296189546585,
-0.08078206330537796,
0.035237353295087814,
-0.016973597928881645,
-0.041780371218919754,
0.006760894320905209,
0.06314007192850113,
-0.007145029027014971,
0.0014817864866927266,
-0.02996709570288658,
-0.014835967682301998,
-0.029585132375359535,
0.021014787256717682,
0.0020088159944862127,
-0.0009219759958796203,
-0.03904377669095993,
-0.0011747014941647649,
-0.07654628157615662,
-0.037929877638816833,
0.06835179775953293,
-0.03502815589308739,
0.023109247907996178,
-0.010909093543887138,
0.006719703320413828,
0.03199121728539467,
-0.010724917985498905,
-0.022612348198890686,
-0.009763107635080814,
0.016415543854236603,
-0.04464758187532425,
-0.03390997275710106,
0.006348858121782541,
0.008433230221271515,
0.010163767263293266,
0.0247089434415102,
-0.06561672687530518,
-0.018087752163410187,
-0.09950710833072662,
-0.03344392776489258,
-0.07249516993761063,
0.010231271386146545,
-0.014288119971752167,
-0.0215179231017828,
0.0630786120891571,
0.0395733080804348,
0.03868331015110016,
-0.02903585694730282,
0.052287813276052475,
0.06018850952386856,
-0.010500791482627392,
-0.012677177786827087,
0.060426440089941025,
-0.022935016080737114,
0.01809549145400524,
0.07927840203046799,
0.006873983424156904,
0.023258697241544724,
-0.029543744400143623,
0.09540389478206635,
-0.029578646644949913,
0.038194600492715836,
0.026387212797999382,
0.046589262783527374,
0.013517637737095356,
-0.017222732305526733,
-0.00248741009272635,
-0.3192667067050934,
-0.01950537972152233,
0.011832051910459995,
-0.01319783553481102,
0.051667362451553345,
-0.061341386288404465,
0.020005691796541214,
-0.05770165100693703,
-0.02143758162856102,
0.007827281020581722,
0.002702190540730953,
0.021556232124567032,
-0.0724259614944458,
0.03804265335202217,
-0.007170114200562239,
0.044326700270175934,
0.05887957289814949,
-0.04783453047275543,
0.026369543746113777,
0.02473370172083378,
0.004725205712020397,
-0.004800444468855858,
0.006820280104875565,
0.014483360573649406,
-0.007133933249861002,
-0.029079770669341087,
0.09418263286352158,
-0.025940092280507088,
-0.0032834557350724936,
-0.03217674419283867,
-0.026234159246087074,
0.052428655326366425,
0.006909240037202835,
-0.12993216514587402,
0.024142900481820107,
-0.013636826537549496,
0.005341797135770321,
-0.06431259214878082,
-0.04758082702755928,
-0.04985658824443817,
-0.09213904291391373,
0.015154099091887474,
-0.016141965985298157,
-0.09937649220228195,
-0.05326220765709877,
-0.0052260723896324635,
0.0117262564599514,
0.0328524149954319,
-0.04367176070809364,
-0.010889182798564434,
-0.0025912451092153788,
0.058666203171014786,
-0.003406998934224248,
-0.008188946172595024,
0.00533746974542737,
-0.04420782998204231,
-0.08124619722366333,
-0.03608907014131546,
-0.028735166415572166,
-0.03308466076850891,
0.022326070815324783,
-0.012248624116182327,
0.07964703440666199,
0.011593432165682316,
0.0025011564139276743,
-0.023781396448612213,
0.015586629509925842,
-0.0006723252008669078,
0.06681901961565018,
-0.00046872938401065767,
0.0010206199949607253,
0.09316133707761765,
-0.04282929375767708,
-0.03371133655309677,
0.09934443980455399,
-0.017360519617795944,
-0.003527974244207144,
0.03241294249892235,
-0.09916305541992188,
-0.02371734380722046,
0.018034901469945908,
-0.020230360329151154,
0.061694931238889694,
0.011775300838053226,
-0.016149921342730522,
-0.004638357553631067,
0.04378151893615723,
-0.03353160247206688,
0.05264575406908989,
0.020518386736512184,
0.031121108680963516,
-0.025399427860975266,
-0.009943870827555656,
-0.01830759458243847,
0.05269816517829895,
0.028285356238484383,
-0.2708987891674042,
0.011222793720662594,
-0.04614743962883949,
0.011904492974281311,
0.027080176398158073,
0.04188684746623039,
0.02516152150928974,
0.022996148094534874,
-0.06697133928537369,
-0.043783288449048996,
-0.008505159988999367,
-0.0010467557003721595,
0.04083575680851936,
-0.003288417123258114,
0.009830096736550331,
-0.034684181213378906,
0.04705150052905083,
-0.016217686235904694,
0.01620357297360897,
0.0034651176538318396,
0.009343631565570831,
0.020104272291064262,
0.12347232550382614,
0.0669318214058876,
0.012089978903532028,
0.046112362295389175,
0.037538327276706696,
-0.024956325069069862,
0.0329122357070446,
0.0000640799553366378,
0.04267846792936325,
0.017362583428621292,
0.05107519030570984,
0.022687355056405067,
0.020477356389164925,
-0.022402381524443626,
-0.06060563400387764,
0.0016790349036455154,
0.00659898342564702,
-0.010500497184693813,
0.004849743098020554,
0.02301984280347824,
0.014194559305906296,
0.00038097388460300863,
0.07513323426246643,
-0.08598312735557556,
-0.014355439692735672,
-0.07084006071090698,
-0.0428021065890789,
0.02142164297401905,
-0.004520160146057606,
-0.033100374042987823,
0.014531328342854977,
0.030243171378970146,
0.07178434729576111,
0.03192918002605438,
-0.012392985634505749,
-0.027150152251124382,
0.026545511558651924,
-0.0008905674912966788,
-0.004829975310713053,
-0.022374115884304047,
-0.007497954647988081,
0.039342910051345825,
0.03281877189874649
] |
PMID:18494
|
Effect of electrical stimulation of the autonomic nerves on the levels and synthesis of cyclic nucleotides in the rat salivary glands: relationship to enhanced growth.
|
Electrical stimulation of either the parasympathetic or the sympathetic nerve supply to the parotid and submaxillary glands increases the intracellular level of cyclic GMP and the rate of DNA synthesis and cell division while only sympathetic stimulation raises cyclic AMP levels. The periods of electrical stimulation inducing hyperplasia also raise the cyclic GMP concentration but there is no similar correlation with changes in cyclic AMP levels. However, the extent of hyperplasia induced by parasympathetic and sympathetic stimulation is not directly related to the size of the increase in cyclic GMP concentration that these treatments produce. Changes in cyclic AMP levels are reflected in altered in vitro adenylate cyclase activity. This activity is raised after 2 min sympathetic stimulation and markedly decreased with 30 min sympathetic or parasympathetic stimulation. Guanylate cyclase activity shows no such changes with nerve stimulation.
|
Effect of electrical stimulation of the autonomic nerves on the levels and synthesis of cyclic nucleotides in the rat salivary glands: relationship to enhanced growth. Electrical stimulation of either the parasympathetic or the sympathetic nerve supply to the parotid and submaxillary glands increases the intracellular level of cyclic GMP and the rate of DNA synthesis and cell division while only sympathetic stimulation raises cyclic AMP levels. The periods of electrical stimulation inducing hyperplasia also raise the cyclic GMP concentration but there is no similar correlation with changes in cyclic AMP levels. However, the extent of hyperplasia induced by parasympathetic and sympathetic stimulation is not directly related to the size of the increase in cyclic GMP concentration that these treatments produce. Changes in cyclic AMP levels are reflected in altered in vitro adenylate cyclase activity. This activity is raised after 2 min sympathetic stimulation and markedly decreased with 30 min sympathetic or parasympathetic stimulation. Guanylate cyclase activity shows no such changes with nerve stimulation.
|
[
0.02150113321840763,
-0.0003564994258340448,
0.03884291276335716,
0.008733640424907207,
-0.005739196203649044,
-0.003315089037641883,
0.013904538936913013,
0.005938214249908924,
-0.019755015149712563,
-0.06787319481372833,
0.02383631467819214,
-0.033759310841560364,
0.002964410465210676,
0.02299010194838047,
-0.0036739036440849304,
0.0009534810087643564,
0.004398147109895945,
-0.009923878125846386,
0.010857105255126953,
-0.011043619364500046,
0.024014415219426155,
-0.03404347598552704,
0.05264626443386078,
-0.017412638291716576,
0.0051581040024757385,
-0.008720609359443188,
-0.010872614569962025,
0.011217465624213219,
-0.07737907022237778,
-0.18622058629989624,
0.06391306966543198,
-0.011225649155676365,
0.008702368475496769,
-0.012548823840916157,
-0.020846782252192497,
-0.04410291090607643,
-0.07134239375591278,
0.04030616953969002,
-0.007765265181660652,
0.05386519432067871,
0.018702158704400063,
0.00513513432815671,
-0.0021785565186291933,
-0.003329810220748186,
0.015372374095022678,
-0.017443686723709106,
-0.05867309123277664,
-0.005150884389877319,
0.07413090020418167,
-0.025705823674798012,
0.05654355138540268,
-0.015897464007139206,
0.01207402441650629,
0.04955783486366272,
-0.005180369596928358,
-0.023523900657892227,
-0.011182588525116444,
0.05500812456011772,
0.022359071299433708,
0.005357931833714247,
-0.004861134570091963,
0.07532370835542679,
-0.15901358425617218,
0.052930403500795364,
0.09257937967777252,
-0.013196272775530815,
-0.039148032665252686,
-0.017186705023050308,
0.025160064920783043,
0.04691394045948982,
-0.06769994646310806,
0.07137411832809448,
-0.00007229274342535064,
-0.023182641714811325,
-0.016437048092484474,
-0.0330745205283165,
-0.06461834907531738,
-0.00035185267915949225,
0.037698790431022644,
0.0358375646173954,
0.027097493410110474,
0.00889558158814907,
0.009741931222379208,
-0.07300270348787308,
0.0010774676920846105,
-0.027799606323242188,
0.031511008739471436,
-0.07025150954723358,
-0.033721454441547394,
0.018496181815862656,
-0.0763515755534172,
-0.02479240484535694,
-0.029175305739045143,
-0.06648947298526764,
-0.01401782501488924,
0.030009420588612556,
-0.028786402195692062,
-0.01354730874300003,
-0.036405500024557114,
0.3365209996700287,
-0.023614509031176567,
-0.020368600264191628,
-0.08264834433794022,
0.018392495810985565,
0.010581952519714832,
-0.005929997190833092,
-0.023161910474300385,
0.018062550574541092,
-0.05526619032025337,
0.06507619470357895,
0.05202115699648857,
-0.017775652930140495,
0.001059338916093111,
-0.03632669523358345,
-0.059388045221567154,
0.013023501262068748,
0.04552586004137993,
0.015455851331353188,
-0.013683770783245564,
0.06503237038850784,
-0.024296188727021217,
-0.027527667582035065,
0.007292858324944973,
-0.03569609671831131,
0.03310780227184296,
0.00900048017501831,
-0.03966944292187691,
0.12234436720609665,
0.07902579009532928,
-0.03512859717011452,
0.04299059137701988,
0.05255412682890892,
-0.055710144340991974,
0.0531783364713192,
0.03957211971282959,
-0.022582795470952988,
0.03948359936475754,
-0.036443617194890976,
0.020608246326446533,
0.01820272207260132,
-0.009723953902721405,
-0.04784196615219116,
-0.031683266162872314,
-0.08245772123336792,
0.03804577514529228,
0.12078877538442612,
-0.026676319539546967,
0.04490065574645996,
-0.03242906928062439,
0.020144933834671974,
-0.0467572957277298,
0.0019529653945937753,
-0.04235675558447838,
-0.001606231788173318,
0.009165185503661633,
0.07259175181388855,
-0.018228337168693542,
-0.0019872006960213184,
0.03804856538772583,
-0.0496855154633522,
-0.08453509956598282,
-0.006302612368017435,
0.0512230210006237,
0.04083510488271713,
0.09768379479646683,
0.004411105532199144,
-0.050489529967308044,
-0.02823135443031788,
0.02998453937470913,
0.021912572905421257,
-0.016334159299731255,
0.037007417529821396,
-0.04462829604744911,
-0.04236633703112602,
0.012361770495772362,
0.013083504512906075,
0.0421234667301178,
-0.022978728637099266,
0.036891546100378036,
0.02317499741911888,
0.031248874962329865,
-0.002536625601351261,
-0.0015295564662665129,
0.013724233955144882,
0.018179455772042274,
0.011981912888586521,
-0.06592100858688354,
-0.008800671435892582,
0.014600255526602268,
0.03733640909194946,
-0.04317755997180939,
-0.027183212339878082,
-0.09985039383172989,
0.024986358359456062,
-0.03128746524453163,
-0.040109746158123016,
0.0022113611921668053,
0.007460137363523245,
0.009621766395866871,
0.01177240815013647,
0.017223380506038666,
-0.013714440166950226,
0.05911097303032875,
0.054865848273038864,
0.006666275206953287,
0.0965864285826683,
-0.04300718009471893,
0.02536861225962639,
0.0590040385723114,
0.0003572181740310043,
0.04155511409044266,
0.022945448756217957,
0.08112695068120956,
0.007392750587314367,
-0.03934701904654503,
0.015718216076493263,
-0.10830901563167572,
0.041815128177404404,
0.08345941454172134,
-0.005402632523328066,
-0.026258381083607674,
-0.02991609275341034,
-0.01917870342731476,
-0.2809637486934662,
-0.09828349202871323,
0.015323021449148655,
-0.037629663944244385,
0.03629309684038162,
-0.020750071853399277,
0.08646178990602493,
-0.05721357464790344,
0.014302492141723633,
-0.07713635265827179,
0.06105462834239006,
0.04915371164679527,
-0.0366031788289547,
-0.017622705549001694,
-0.00840021576732397,
0.019515858963131905,
0.012345213443040848,
-0.001559156458824873,
0.030462458729743958,
0.034032661467790604,
0.02072705700993538,
0.04626806080341339,
0.014195718802511692,
-0.0027328652795404196,
-0.01694149151444435,
-0.026148416101932526,
0.06651654094457626,
0.011653009802103043,
-0.05061126500368118,
0.012390553019940853,
0.021829979494214058,
0.01342097483575344,
-0.02877023071050644,
-0.13980117440223694,
-0.034463901072740555,
-0.024336006492376328,
-0.005457565654069185,
0.06923166662454605,
-0.044022563844919205,
-0.031856242567300797,
-0.07202865928411484,
0.039098192006349564,
-0.03144152835011482,
0.009645197540521622,
-0.0598805733025074,
-0.040106117725372314,
0.04477131739258766,
0.04303513467311859,
0.002568536438047886,
-0.07139867544174194,
0.031000744551420212,
0.015962043777108192,
-0.027256298810243607,
-0.04076742008328438,
0.016451634466648102,
-0.04619648680090904,
-0.057606566697359085,
-0.01214078813791275,
-0.09686359018087387,
-0.02557830512523651,
0.052611589431762695,
0.025076275691390038,
0.0080482829362154,
-0.005935645196586847,
-0.020243283361196518,
0.07249859720468521,
-0.005031343549489975,
0.000963586731813848,
0.028270144015550613,
0.03725239261984825,
-0.0052134632132947445,
0.1417684704065323,
0.007835818454623222,
0.022620879113674164,
0.028488174080848694,
-0.008930276148021221,
-0.04532588645815849,
-0.029659539461135864,
-0.07459395378828049,
0.01770254597067833,
-0.051015667617321014,
-0.03911373391747475,
0.0846329852938652,
-0.0006638382910750806,
-0.06686563044786453,
0.00690654618665576,
-0.004424878396093845,
0.0028688593301922083,
0.05287947878241539,
-0.022839535027742386,
0.02133001945912838,
-0.007906612940132618,
-0.03360217437148094,
0.00120168284047395,
0.03981455788016319,
0.014168537221848965,
-0.2768377661705017,
0.009458228014409542,
0.04057057574391365,
0.007196241058409214,
-0.054909300059080124,
0.061271339654922485,
0.007824983447790146,
-0.019535215571522713,
-0.05317213013768196,
0.07867829501628876,
0.02102329023182392,
0.02407756634056568,
0.06466363370418549,
0.08205348998308182,
0.009881273843348026,
0.037952546030282974,
0.005247246008366346,
-0.018321217969059944,
0.025067191570997238,
-0.017082644626498222,
-0.01150862779468298,
-0.0025418156292289495,
0.1688586324453354,
0.03658270090818405,
0.007118101231753826,
0.01499927043914795,
-0.00462042773142457,
0.019982313737273216,
0.0039442856796085835,
-0.013884362764656544,
-0.04892783239483833,
0.03904622048139572,
0.024630112573504448,
-0.020377518609166145,
0.07008055597543716,
0.04804643988609314,
-0.028203699737787247,
0.0114606823772192,
0.00523475231602788,
-0.012646935880184174,
-0.010866985656321049,
0.01464784238487482,
-0.04544738680124283,
-0.0020535914227366447,
0.07539576292037964,
-0.08553851395845413,
-0.02645128406584263,
-0.044649407267570496,
0.032200682908296585,
0.011835242621600628,
-0.036474768072366714,
0.002531875856220722,
0.06336246430873871,
0.040091224014759064,
-0.03385666012763977,
-0.03340459242463112,
-0.06472659856081009,
0.011123061180114746,
0.07144361734390259,
0.023219479247927666,
0.013905498199164867,
-0.02205606736242771,
-0.012926517985761166,
0.06643334031105042,
-0.000462721218355
] |
PMID:18496
|
Fetal and maternal plasma electrolytes, blood gases, and pH in dairy cows during late gestation.
|
Sodium, potassium, and chloride were determined in fetal and maternal plasma of six cows between 208 and 269 days of gestation. Blood pH and partial pressures of oxygen and carbon dioxide also were measured in samples collected from the jugular vein of the cow and the caudal aorta and vena cava of the fetus. Concentration of chloride in fetal plasma was generally lower than the corresponding maternal value, and fetal potassium was greater than maternal. Potassium of fetal plasma increased towards term. Sodium concentrations were not significantly different between fetal and maternal plasms. Once fetal and maternal pH and blood gas tensions had stabilized after surgery, there was little change until shortly before delivery. In the last 24 h before calving maternal partial pressure of oxygen was high and fetal blood pH was low.
|
Fetal and maternal plasma electrolytes, blood gases, and pH in dairy cows during late gestation. Sodium, potassium, and chloride were determined in fetal and maternal plasma of six cows between 208 and 269 days of gestation. Blood pH and partial pressures of oxygen and carbon dioxide also were measured in samples collected from the jugular vein of the cow and the caudal aorta and vena cava of the fetus. Concentration of chloride in fetal plasma was generally lower than the corresponding maternal value, and fetal potassium was greater than maternal. Potassium of fetal plasma increased towards term. Sodium concentrations were not significantly different between fetal and maternal plasms. Once fetal and maternal pH and blood gas tensions had stabilized after surgery, there was little change until shortly before delivery. In the last 24 h before calving maternal partial pressure of oxygen was high and fetal blood pH was low.
|
[
-0.003661577356979251,
0.0064105382189154625,
0.030482150614261627,
0.025551803410053253,
0.02582528442144394,
-0.006535172462463379,
0.05848962068557739,
0.031134020537137985,
0.00161563022993505,
0.034928351640701294,
-0.036200180649757385,
-0.1038326770067215,
-0.043216824531555176,
0.013722050935029984,
-0.02758944220840931,
-0.04874896630644798,
-0.014659740030765533,
-0.019957978278398514,
-0.1314673274755478,
0.024352477863430977,
0.029261471703648567,
0.016203617677092552,
0.004923101514577866,
-0.032812364399433136,
0.09655636548995972,
0.012301872484385967,
0.012175614945590496,
0.0034982592333108187,
-0.041783407330513,
-0.15362226963043213,
-0.05537280812859535,
0.007195209618657827,
0.014263785444200039,
0.01929781213402748,
0.00410881545394659,
0.022508088499307632,
0.031041424721479416,
0.0332689993083477,
-0.04632608965039253,
0.08672096580266953,
0.02854260243475437,
0.01011242438107729,
0.001025942387059331,
0.02220892533659935,
-0.03231385722756386,
-0.022565670311450958,
0.02641998790204525,
0.037194378674030304,
0.07950539886951447,
0.027564696967601776,
0.0200664009898901,
0.011078712530434132,
0.0003124889626633376,
0.03581388294696808,
-0.0057362038642168045,
-0.00412064278498292,
0.034312132745981216,
-0.013396812602877617,
0.020032726228237152,
0.00410687830299139,
0.008553232997655869,
0.034612007439136505,
-0.12824466824531555,
0.0789879560470581,
0.03572061285376549,
0.01943306066095829,
-0.055152252316474915,
-0.027286967262625694,
0.059929259121418,
0.03923851624131203,
-0.03137201815843582,
0.050050001591444016,
0.0450030118227005,
0.0021881351713091135,
0.03670720383524895,
-0.020426515489816666,
0.059794068336486816,
-0.03062685951590538,
0.03052603453397751,
-0.019603395834565163,
-0.07432690262794495,
0.01853867992758751,
-0.008671402931213379,
-0.014799017459154129,
-0.021806836128234863,
-0.027307163923978806,
0.0030699276831001043,
-0.02079116739332676,
0.00417568301782012,
0.00556214852258563,
-0.02683434821665287,
-0.0861477255821228,
-0.018402233719825745,
0.040002238005399704,
-0.02054625004529953,
0.012530614621937275,
0.0044480739161372185,
-0.03434550762176514,
-0.01286801416426897,
0.41615772247314453,
-0.04712206497788429,
0.010137884877622128,
-0.03161943703889847,
-0.023175768554210663,
-0.02975003980100155,
0.03152384236454964,
0.00342370942234993,
0.06015433743596077,
-0.04571710526943207,
0.03592103347182274,
-0.0023599525447934866,
0.025140756741166115,
0.05163086578249931,
-0.0924442708492279,
-0.03214292973279953,
-0.03024505451321602,
0.02621772326529026,
-0.000027441516067483462,
0.04516910761594772,
0.09600360691547394,
-0.004401561804115772,
-0.029050147160887718,
0.03609074652194977,
-0.07879714667797089,
0.09363705664873123,
-0.011739790439605713,
0.012241998687386513,
0.05134648084640503,
-0.022589394822716713,
0.007579343859106302,
0.012718033976852894,
0.0259038507938385,
-0.08016720414161682,
0.007343512028455734,
-0.0014587495243176818,
-0.04127274826169014,
-0.013161517679691315,
-0.03092939220368862,
0.009736496955156326,
-0.01105187926441431,
-0.034263789653778076,
-0.06802936643362045,
-0.05454205349087715,
-0.10048874467611313,
-0.012547407299280167,
0.09220925718545914,
0.03907424584031105,
0.049120236188173294,
0.00013628556916955858,
-0.027703406289219856,
-0.032822951674461365,
0.0691361054778099,
0.005308872554451227,
0.03156988322734833,
0.028603507205843925,
0.004464796278625727,
-0.07202528417110443,
0.03437570109963417,
0.011669685132801533,
-0.04479224979877472,
0.01375160738825798,
-0.06822711229324341,
-0.006662370637059212,
0.04571661725640297,
0.019511185586452484,
0.005108253564685583,
-0.010395649820566177,
0.006589191500097513,
0.051227301359176636,
-0.022364381700754166,
0.053476862609386444,
0.009934472851455212,
-0.0208706334233284,
-0.10430965572595596,
0.032603416591882706,
-0.027163464576005936,
0.01766808144748211,
0.08723669499158859,
-0.010670526884496212,
-0.0145215829834342,
0.04404502734541893,
0.007841632701456547,
0.02287112921476364,
0.015031584538519382,
0.0654873177409172,
-0.05454272776842117,
-0.06463056802749634,
0.015422046184539795,
-0.00011409778380766511,
0.1010490357875824,
0.013966383412480354,
-0.03493431210517883,
-0.019683051854372025,
-0.0002732368593569845,
-0.060636647045612335,
-0.033036112785339355,
0.06137387827038765,
-0.01345576997846365,
0.014935406856238842,
-0.05759963393211365,
-0.0031474754214286804,
-0.036443255841732025,
-0.01611812226474285,
0.07672383636236191,
0.05521217733621597,
0.022718893364071846,
-0.01174048613756895,
0.007085956633090973,
0.0405302569270134,
-0.019947124645113945,
-0.011006849817931652,
0.05246186628937721,
-0.027984879910945892,
-0.034064777195453644,
-0.07822833955287933,
0.009003466926515102,
-0.014951089397072792,
-0.01196358259767294,
0.035305656492710114,
0.0018907408230006695,
0.09784861654043198,
-0.09133415669202805,
0.03353251516819,
-0.29858601093292236,
0.004667204804718494,
0.0593051016330719,
0.018850460648536682,
0.03698539733886719,
-0.023000279441475868,
0.0031894638668745756,
-0.04722223058342934,
0.00021877569088246673,
-0.028345264494419098,
0.05707540735602379,
0.049217745661735535,
-0.052226364612579346,
0.035817477852106094,
0.016204386949539185,
0.03927658125758171,
0.024219267070293427,
-0.05285142362117767,
0.062161292880773544,
0.08368425071239471,
-0.028878342360258102,
-0.02029123343527317,
0.04777517914772034,
0.05560235306620598,
0.04242052510380745,
0.024556821212172508,
0.1061413362622261,
-0.047369059175252914,
0.03201817348599434,
-0.0758734717965126,
-0.028779763728380203,
0.031929291784763336,
-0.0033805116545408964,
-0.04766454920172691,
-0.013003706000745296,
-0.00872861035168171,
-0.04130358621478081,
0.035400912165641785,
-0.031164713203907013,
-0.0014961600536480546,
-0.049822110682725906,
0.0023295388091355562,
-0.008845608681440353,
0.007747752126306295,
-0.020014870911836624,
0.029162364080548286,
0.014573849737644196,
-0.02059098519384861,
-0.010644453577697277,
-0.03178318589925766,
0.037067633122205734,
-0.01902695745229721,
-0.02278939262032509,
-0.07570258527994156,
0.027465710416436195,
0.006672575604170561,
-0.024420147761702538,
0.0026384040247648954,
-0.07025854289531708,
-0.011771566234529018,
0.01084657572209835,
-0.017118431627750397,
-0.03908513858914375,
-0.013555140234529972,
-0.04853430017828941,
0.005313542205840349,
0.017563169822096825,
0.003598050447180867,
0.016555681824684143,
-0.018097396939992905,
-0.007322604302316904,
0.06932590901851654,
-0.010508048348128796,
-0.028557734563946724,
0.029770810157060623,
0.03758611902594566,
-0.049180179834365845,
-0.0594836100935936,
-0.022806229069828987,
0.019479084759950638,
0.06129677593708038,
-0.07095851004123688,
0.05348224565386772,
-0.007205028552561998,
0.035551249980926514,
0.04252133518457413,
0.03504001349210739,
-0.02180461958050728,
-0.016867119818925858,
-0.0020046138670295477,
-0.03810619190335274,
-0.004064070992171764,
-0.03803789243102074,
-0.024569684639573097,
0.011501539498567581,
0.023095302283763885,
-0.2626800835132599,
-0.009337667375802994,
0.0016493004513904452,
-0.029788529500365257,
0.01596473716199398,
0.00830596499145031,
0.005054676439613104,
-0.06603167206048965,
-0.011648626066744328,
0.04034525156021118,
-0.003229529596865177,
0.029356123879551888,
0.06921493262052536,
0.027164900675415993,
0.04864371567964554,
0.06959924846887589,
0.006953504402190447,
-0.07141213119029999,
-0.05714024603366852,
-0.004172254353761673,
0.01825394667685032,
-0.07736019790172577,
0.13258355855941772,
-0.0052383411675691605,
-0.056967318058013916,
-0.016952520236372948,
0.012390262447297573,
0.006752354558557272,
0.027394603937864304,
0.03454853221774101,
0.01249243225902319,
0.02907428704202175,
0.02283862605690956,
0.0011697326553985476,
0.012670219875872135,
0.04653070867061615,
-0.03025674819946289,
-0.009838652797043324,
0.043621040880680084,
-0.03480914980173111,
0.021799525246024132,
-0.02020285464823246,
-0.04285946860909462,
-0.09751063585281372,
0.06526129692792892,
-0.06383553892374039,
-0.0160810723900795,
-0.05103675648570061,
0.06846171617507935,
-0.019363293424248695,
-0.07690824568271637,
0.0122010363265872,
0.031006960198283195,
-0.00747810211032629,
0.03229798376560211,
0.0012953730765730143,
0.02747885137796402,
-0.01485810149461031,
-0.056116700172424316,
-0.040726978331804276,
0.02619604393839836,
0.008077271282672882,
-0.023331189528107643,
0.05183892697095871,
0.06925061345100403
] |
PMID:18540
|
Biochemical and histocytochemical studies on response of ammonia-producing enzymes for nh4cl-induced acidosis.
|
NH4Cl-induced acidosis in rats resulted in renal enlargement and increase in activities of phosphate-dependent glutaminase and glutamic dehydrogenase. The renal enlargement was associated with protein synthesis but not deoxyribonucleic acid synthesis. In control rats histochemical activity of glutamic dehydrogenase was seen dominantly in the proximal straight tubule. In acidotic rats high activity was noted in the proximal convoluted tubule as well as in the proximal straight tubule. By electron microscopy reaction product was in mitochondria. The results suggest that urine ammonia is produced in mitochondria of epithelial cells in the proximal straight tubule in both normal and acidotic rats. Increased enzyme activity in acidotic rats is largely associated with epithelial cells of the proximal convoluted tubule.
|
Biochemical and histocytochemical studies on response of ammonia-producing enzymes for nh4cl-induced acidosis. NH4Cl-induced acidosis in rats resulted in renal enlargement and increase in activities of phosphate-dependent glutaminase and glutamic dehydrogenase. The renal enlargement was associated with protein synthesis but not deoxyribonucleic acid synthesis. In control rats histochemical activity of glutamic dehydrogenase was seen dominantly in the proximal straight tubule. In acidotic rats high activity was noted in the proximal convoluted tubule as well as in the proximal straight tubule. By electron microscopy reaction product was in mitochondria. The results suggest that urine ammonia is produced in mitochondria of epithelial cells in the proximal straight tubule in both normal and acidotic rats. Increased enzyme activity in acidotic rats is largely associated with epithelial cells of the proximal convoluted tubule.
|
[
0.00648545240983367,
0.0014671038370579481,
-0.00042502456926740706,
0.022144367918372154,
0.011107968166470528,
-0.03643395006656647,
0.0790712758898735,
0.04420613497495651,
-0.03945324569940567,
0.00801629014313221,
0.008402262814342976,
-0.07664051651954651,
0.045020103454589844,
0.03797547146677971,
-0.04750471189618111,
0.004577617160975933,
-0.012578370980918407,
0.043413881212472916,
-0.08689051866531372,
-0.040712129324674606,
-0.012013806961476803,
0.012491735629737377,
0.08170977979898453,
-0.0392046757042408,
0.019106509163975716,
-0.026351971551775932,
0.03675136715173721,
0.03138640522956848,
-0.05626813694834709,
-0.1705275923013687,
0.0508127361536026,
0.048937730491161346,
-0.01101095974445343,
-0.060565412044525146,
0.018436141312122345,
0.02062336727976799,
-0.03799363970756531,
0.03335646912455559,
-0.009208830073475838,
0.03251414746046066,
0.007805126719176769,
0.012189602479338646,
0.03747514635324478,
0.0655883401632309,
0.03215983137488365,
0.03226352855563164,
-0.04811700060963631,
0.010748917236924171,
0.08939160406589508,
0.007834212854504585,
0.023192958906292915,
-0.038501009345054626,
0.06979481875896454,
-0.0018204988446086645,
0.029805060476064682,
-0.05924253910779953,
0.05310114473104477,
0.04598386585712433,
-0.014357767067849636,
-0.020395342260599136,
0.009385225363075733,
0.012378170154988766,
-0.12310667335987091,
0.04486551135778427,
0.016111284494400024,
0.005979039240628481,
-0.0137469582259655,
-0.06386478990316391,
0.01694979891180992,
0.014455904252827168,
-0.06320770829916,
0.03897157683968544,
-0.027999067679047585,
0.014640990644693375,
-0.036458518356084824,
-0.01691841147840023,
-0.010906083509325981,
0.017648618668317795,
0.010960887186229229,
0.02887534536421299,
-0.006714142858982086,
0.0073816897347569466,
0.028328800573945045,
0.01083449088037014,
0.017563657835125923,
-0.029918663203716278,
-0.01829223707318306,
-0.016800764948129654,
-0.004158845171332359,
-0.02155349962413311,
-0.05726546794176102,
-0.02391771227121353,
-0.015318948775529861,
-0.019541554152965546,
-0.06191468983888626,
0.023060204461216927,
0.05030834302306175,
-0.04349575564265251,
0.006401472259312868,
0.3673582375049591,
-0.01251203753054142,
0.013491506688296795,
-0.033812593668699265,
-0.018119292333722115,
-0.02754300832748413,
0.05911380797624588,
-0.03237210214138031,
0.025713080540299416,
-0.016347022727131844,
0.08242131769657135,
0.08922628313302994,
0.023734234273433685,
0.016245288774371147,
-0.06597791612148285,
-0.025933604687452316,
-0.0046128942631185055,
0.04591495916247368,
0.017857924103736877,
-0.053318943828344345,
0.06716887652873993,
0.022107362747192383,
-0.0038434057496488094,
0.011989901773631573,
-0.043890535831451416,
0.016752539202570915,
0.02232900634407997,
-0.032675277441740036,
0.09156719595193863,
0.029524942860007286,
-0.02015463076531887,
-0.011318149045109749,
0.050934016704559326,
0.021173397079110146,
0.0030725214164704084,
0.014508616179227829,
-0.01658657379448414,
0.06405311077833176,
-0.06539256870746613,
0.04542703554034233,
0.07138469815254211,
-0.06998349726200104,
-0.056850798428058624,
-0.11658679693937302,
-0.07004551589488983,
-0.018294425681233406,
0.0862937793135643,
0.020705480128526688,
-0.011175055056810379,
-0.0037233561743050814,
0.00408320315182209,
0.04377584159374237,
0.061368633061647415,
-0.03326767310500145,
0.009563065133988857,
0.02356908656656742,
0.01755506917834282,
-0.03561108931899071,
0.010810060426592827,
0.03040853515267372,
-0.05793757364153862,
-0.07791657000780106,
-0.045264557003974915,
-0.03177911043167114,
0.04888547584414482,
0.06640602648258209,
0.05743483081459999,
-0.06512314826250076,
-0.001032081083394587,
0.0013409322127699852,
0.013405675068497658,
-0.03414412587881088,
0.029149694368243217,
-0.033217113465070724,
-0.00426213163882494,
-0.021833224222064018,
0.002017197199165821,
0.022213324904441833,
0.026360100135207176,
-0.004027951508760452,
-0.04654737934470177,
0.014661397784948349,
-0.04690006375312805,
-0.03651830554008484,
0.019899630919098854,
0.046056993305683136,
-0.04104942828416824,
-0.06158321723341942,
0.04560460150241852,
0.05124429985880852,
0.01801319047808647,
-0.05367660894989967,
0.027854591608047485,
-0.11919720470905304,
-0.021726125851273537,
0.015116817317903042,
-0.0684015154838562,
-0.023642608895897865,
-0.008580348454415798,
-0.011330037377774715,
0.030148953199386597,
0.13532328605651855,
0.0326036661863327,
0.018853748217225075,
0.04244093969464302,
-0.04122527316212654,
-0.0011174635728821158,
-0.01630556955933571,
0.06000998988747597,
0.07651156932115555,
-0.011134648695588112,
-0.09057534486055374,
-0.03190669044852257,
-0.054484523832798004,
0.022015085443854332,
-0.045403171330690384,
0.02108810469508171,
0.018039096146821976,
0.0014926822623237967,
0.0583135187625885,
-0.025596147403120995,
0.05026590824127197,
-0.06867748498916626,
-0.032795973122119904,
-0.2896260917186737,
-0.031104568392038345,
0.019430192187428474,
-0.04382053390145302,
0.053794920444488525,
-0.04663640633225441,
0.027471715584397316,
-0.05663646012544632,
-0.0012610516278073192,
-0.04113581404089928,
0.042708415538072586,
0.01157937478274107,
0.005305793602019548,
-0.03923451527953148,
0.009972672909498215,
0.05109429731965065,
0.020700868219137192,
-0.011696954257786274,
0.04361126199364662,
0.05258810892701149,
0.06286223977804184,
-0.013177636079490185,
0.003136837389320135,
0.022357376292347908,
0.05106007307767868,
-0.01787574402987957,
0.1066104918718338,
-0.005374400410801172,
0.020075548440217972,
-0.04360486939549446,
0.03800424933433533,
-0.0082344189286232,
-0.061527226120233536,
-0.11140081286430359,
-0.013605715706944466,
-0.010504092089831829,
-0.048329900950193405,
-0.01221030205488205,
-0.06332998722791672,
-0.0074141547083854675,
-0.06155607849359512,
-0.014086437411606312,
-0.06479457765817642,
-0.06723665446043015,
-0.008965562097728252,
0.009254641830921173,
0.02429605834186077,
0.005466867703944445,
0.03034215047955513,
-0.06708677858114243,
0.03970027714967728,
0.0019970876164734364,
-0.016919847577810287,
-0.04497323930263519,
0.0003046119527425617,
-0.01772531308233738,
-0.03105265088379383,
-0.016317233443260193,
-0.06756243854761124,
0.007711980491876602,
-0.004251228645443916,
0.02321341075003147,
-0.007372270338237286,
0.02106960117816925,
0.0219461377710104,
0.01924317143857479,
-0.03837834671139717,
-0.002579710679128766,
-0.009593123570084572,
0.030791722238063812,
0.03654558211565018,
0.11361332982778549,
0.023479081690311432,
0.06657392531633377,
0.0739416852593422,
0.06247962266206741,
-0.048283226788043976,
-0.05611099302768707,
-0.031598929315805435,
0.020545681938529015,
-0.037963803857564926,
-0.10628359764814377,
0.06828097999095917,
-0.012141656130552292,
0.021069204434752464,
0.07060984522104263,
-0.0036253042053431273,
-0.034176092594861984,
0.019930515438318253,
-0.006166191305965185,
0.06792381405830383,
0.02010117471218109,
-0.03113831952214241,
-0.02323661558330059,
0.006560280453413725,
-0.012079974636435509,
-0.2774235010147095,
0.010986587963998318,
0.031489595770835876,
-0.03524467721581459,
-0.026044493541121483,
0.031355611979961395,
-0.0389375165104866,
0.01243227906525135,
0.010763252153992653,
0.03151017799973488,
0.06989840418100357,
0.040528662502765656,
0.030391084030270576,
-0.007953496649861336,
0.024394355714321136,
0.07465991377830505,
0.00007500395440729335,
-0.02774064429104328,
0.015021338127553463,
0.006965418811887503,
0.026020754128694534,
-0.05201590061187744,
0.15003681182861328,
0.01855938509106636,
0.014537542127072811,
0.08267485350370407,
-0.03250324726104736,
0.01667889393866062,
-0.004651861265301704,
0.025994937866926193,
-0.016925662755966187,
0.045701026916503906,
0.04022582620382309,
-0.006042941007763147,
0.004146680235862732,
0.028351817280054092,
-0.03455653786659241,
0.036530472338199615,
-0.0063047949224710464,
-0.0004233197250869125,
-0.05676095560193062,
-0.0030360345263034105,
-0.06379872560501099,
-0.013675226829946041,
0.08267875015735626,
-0.036831408739089966,
-0.05516602471470833,
-0.03183171525597572,
0.025972677394747734,
0.009507982060313225,
-0.013022643513977528,
-0.04996208846569061,
-0.005323965568095446,
0.07261589169502258,
-0.013619635254144669,
-0.0451204739511013,
-0.011896959505975246,
-0.06606493145227432,
0.017868153750896454,
0.016744110733270645,
0.03017289750277996,
0.013137760572135448,
-0.017450151965022087,
0.042196791619062424,
-0.001974039012566209
] |
PMID:18541
|
Bunyavirus isolations from mosquitoes in the western Canadian Arctic.
|
Strains of California encephalitis virus (snowshoe hare subtype) were isolated from 8 of 475 pools comprising 23 747 unengorged female mosquitoes of five species collected at three of six locations throughout the Mackenzie Valley of the Northwest Territories, Canada, from latitudes 60 to 69 degrees N between 10 and 24 July 1976. Minimum field infection rates included 1:2734 for Aedes communis, 1:256 to 1:3662 for A. hexodontus and 1:911 to 1:1611 for A. punctor. Northway virus was also isolated from 1 of 3662 A. hexodontus mosquitoes collected at Inuvik (69 degrees N, 135 degrees W). Transmission of CE virus by A. communis infected by feeding on virus in defibrinated blood and incubation at 0, 13 and 23 degrees C for 13-20 days clearly demonstrates the importance of this species as a natural vector, and transmission of CE virus by Culiseta inornata after incubation at 0 and 13 degrees C following intrathoracic injection strengthens evidence of its role as a natural vector. Immunofluorescence was less reliable than imunoperoxidase for detection of CE viral antigen in mosquito salivary glands.
|
Bunyavirus isolations from mosquitoes in the western Canadian Arctic. Strains of California encephalitis virus (snowshoe hare subtype) were isolated from 8 of 475 pools comprising 23 747 unengorged female mosquitoes of five species collected at three of six locations throughout the Mackenzie Valley of the Northwest Territories, Canada, from latitudes 60 to 69 degrees N between 10 and 24 July 1976. Minimum field infection rates included 1:2734 for Aedes communis, 1:256 to 1:3662 for A. hexodontus and 1:911 to 1:1611 for A. punctor. Northway virus was also isolated from 1 of 3662 A. hexodontus mosquitoes collected at Inuvik (69 degrees N, 135 degrees W). Transmission of CE virus by A. communis infected by feeding on virus in defibrinated blood and incubation at 0, 13 and 23 degrees C for 13-20 days clearly demonstrates the importance of this species as a natural vector, and transmission of CE virus by Culiseta inornata after incubation at 0 and 13 degrees C following intrathoracic injection strengthens evidence of its role as a natural vector. Immunofluorescence was less reliable than imunoperoxidase for detection of CE viral antigen in mosquito salivary glands.
|
[
0.024840276688337326,
-0.05018738657236099,
0.014941960573196411,
0.015009751543402672,
0.09063991159200668,
-0.0201712753623724,
0.062410224229097366,
0.08216243982315063,
-0.05731566995382309,
0.0492931604385376,
0.0008914096397347748,
-0.050466056913137436,
0.043341390788555145,
-0.027286674827337265,
-0.015340164303779602,
-0.023081818595528603,
-0.02846500463783741,
-0.061107177287340164,
-0.005154668353497982,
0.07755620777606964,
-0.023745810613036156,
-0.022507036104798317,
0.030082743614912033,
-0.01623707450926304,
-0.02465307153761387,
0.00031483694328926504,
0.009125779382884502,
-0.04892474785447121,
0.017742596566677094,
-0.16889873147010803,
-0.07946217060089111,
0.00039875105721876025,
-0.0011257361620664597,
-0.012407612055540085,
-0.03369327634572983,
-0.0052000973373651505,
0.022813186049461365,
-0.04142420366406441,
-0.02660592459142208,
-0.0014624999603256583,
0.09141264110803604,
0.02644396387040615,
0.0028424356132745743,
0.014301626943051815,
-0.01311369426548481,
-0.04479438439011574,
-0.06061815097928047,
0.04860544577240944,
0.04016947001218796,
-0.040490567684173584,
0.050593726336956024,
-0.03328052535653114,
-0.019165869802236557,
0.009097098372876644,
0.005708359181880951,
0.04041704908013344,
-0.015031128190457821,
-0.02866833470761776,
0.02837442047894001,
0.04090220481157303,
0.0025848206132650375,
0.03779973089694977,
-0.15526539087295532,
0.04122854024171829,
-0.0066456543281674385,
0.004038629122078419,
-0.016969896852970123,
-0.053997624665498734,
-0.013231167569756508,
-0.015293442644178867,
-0.01389206014573574,
0.052822697907686234,
-0.01306170504540205,
0.03371606394648552,
-0.01839093677699566,
0.009094556793570518,
-0.026590045541524887,
0.01199058536440134,
0.02082054875791073,
0.0023011337034404278,
-0.004301346372812986,
0.029348986223340034,
0.09130008518695831,
0.018745752051472664,
0.03420121222734451,
-0.002079280558973551,
0.0692484974861145,
-0.020128408446907997,
0.010259763337671757,
0.009355139918625355,
-0.001268581603653729,
-0.023585161194205284,
-0.006284244824200869,
-0.04392039403319359,
-0.053365614265203476,
0.014035556465387344,
0.06524446606636047,
-0.02314825728535652,
0.04610250145196915,
0.4082690477371216,
-0.039549849927425385,
-0.047114428132772446,
-0.056573908776044846,
0.013081983663141727,
0.0485432930290699,
0.0040698000229895115,
-0.03280796855688095,
-0.03599322587251663,
0.016257233917713165,
0.042842280119657516,
0.03908512741327286,
-0.002219898160547018,
0.034135475754737854,
0.0008632201352156699,
-0.06448362022638321,
0.0009490938391536474,
0.053373001515865326,
0.007624519523233175,
-0.01209013257175684,
0.05160865932703018,
0.013954085297882557,
-0.02594761736690998,
0.03070865385234356,
0.0036583172623068094,
0.07145573198795319,
0.01221312116831541,
-0.014375729486346245,
0.10299491137266159,
0.06759621948003769,
-0.01828206703066826,
0.0796104446053505,
0.021753158420324326,
0.01593356765806675,
-0.028057241812348366,
0.004840841982513666,
-0.028380917385220528,
0.012966157868504524,
0.014501819387078285,
-0.0031344296876341105,
-0.037053484469652176,
-0.029721476137638092,
-0.05204639956355095,
0.021735018119215965,
-0.05065247789025307,
-0.04932917654514313,
0.017414811998605728,
-0.00827123410999775,
-0.00018607554375194013,
-0.07323238253593445,
-0.017283597961068153,
0.0418676882982254,
0.06688490509986877,
-0.006963567342609167,
-0.024676885455846786,
0.036667510867118835,
0.0028159674257040024,
0.006013236008584499,
0.024178247898817062,
-0.08890632539987564,
0.026767974719405174,
-0.013733952306210995,
0.010618041269481182,
0.014198161661624908,
0.05331461504101753,
0.04669243097305298,
-0.049049872905015945,
-0.018627488985657692,
0.043387845158576965,
0.016719607636332512,
0.030414175242185593,
0.016152294352650642,
-0.01646278239786625,
0.017684854567050934,
0.042313102632761,
-0.04137462377548218,
-0.05224437639117241,
-0.08486968278884888,
-0.012636072933673859,
0.02338641881942749,
0.04069468006491661,
-0.02959284372627735,
-0.07966616749763489,
-0.01726195402443409,
0.09133264422416687,
-0.026214424520730972,
-0.01671406999230385,
-0.05478106066584587,
-0.005546088796108961,
0.057277943938970566,
-0.04944959282875061,
0.005055188667029142,
-0.04876838997006416,
0.01630301959812641,
0.008027032017707825,
0.004093767609447241,
0.008768224157392979,
0.01865522935986519,
-0.014218064956367016,
0.09133846312761307,
-0.03532290458679199,
0.09545791894197464,
-0.027003629133105278,
-0.009024685248732567,
0.024689575657248497,
-0.03750362619757652,
0.003322903299704194,
0.012096586637198925,
-0.04920104518532753,
-0.03659370169043541,
0.02550528757274151,
0.0272161103785038,
-0.02375839650630951,
-0.028696365654468536,
-0.03909744694828987,
-0.04907931387424469,
0.05948175489902496,
-0.0030599196907132864,
0.0004319894651416689,
0.07924004644155502,
0.027818450704216957,
0.03905520215630531,
-0.04493153840303421,
-0.005674050655215979,
-0.2876807749271393,
-0.047254569828510284,
-0.014775261282920837,
-0.045520082116127014,
0.05544736236333847,
-0.012861632741987705,
0.006294806022197008,
-0.021427160128951073,
0.06441742181777954,
0.013228579424321651,
0.039775993674993515,
0.038677334785461426,
-0.014981281012296677,
-0.007596808020025492,
0.002351769246160984,
0.10126740485429764,
0.10497531294822693,
-0.01118395198136568,
0.009567146189510822,
0.026572857052087784,
0.0317220538854599,
-0.02631026692688465,
-0.024939369410276413,
0.018093347549438477,
-0.0065642730332911015,
-0.06019645184278488,
0.13712583482265472,
0.00861753337085247,
0.07566599547863007,
-0.0911758542060852,
-0.017277104780077934,
-0.015859944745898247,
0.0014272897969931364,
-0.0584389865398407,
-0.0057143522426486015,
-0.03072991408407688,
-0.025774763897061348,
-0.03253381326794624,
-0.00006832508370280266,
-0.014221470803022385,
-0.016696752980351448,
-0.012084445916116238,
0.0067215654999017715,
0.04277944192290306,
0.02897794358432293,
-0.0774516761302948,
-0.01581953838467598,
0.04533718526363373,
0.07136940211057663,
-0.030712353065609932,
-0.0031877653673291206,
-0.007704577408730984,
0.007402902934700251,
0.04454340413212776,
-0.0024194123689085245,
0.0008838486974127591,
-0.03378480672836304,
0.07192031294107437,
-0.0022135667968541384,
0.07059639692306519,
-0.019876187667250633,
-0.01619216427206993,
-0.02395961806178093,
-0.030550096184015274,
0.007578421849757433,
-0.006003917660564184,
0.019347429275512695,
-0.010477293282747269,
0.07706968486309052,
-0.0018081322778016329,
-0.036626026034355164,
0.09950130432844162,
-0.01286031398922205,
0.006906523834913969,
0.00861175823956728,
0.03298491612076759,
0.027814436703920364,
-0.07357706874608994,
-0.05100627243518829,
0.0021158596500754356,
0.05206353962421417,
-0.08229369670152664,
0.0004614516510628164,
0.022762296721339226,
-0.0012204094091430306,
0.021188024431467056,
-0.031224166974425316,
0.025999462231993675,
0.0046899588778615,
-0.0005262044724076986,
0.05556152015924454,
0.005199962295591831,
-0.016309792175889015,
-0.06893697381019592,
0.006108000408858061,
0.0699717104434967,
-0.25971540808677673,
-0.02190389111638069,
0.02224615588784218,
-0.03288140520453453,
0.02418326586484909,
0.03068038448691368,
0.013850688003003597,
-0.0734306126832962,
-0.002901611616834998,
0.013280266895890236,
0.005598186049610376,
0.008203971199691296,
0.008449695073068142,
0.038310837000608444,
0.02565561607480049,
0.04751897230744362,
0.008713308721780777,
-0.05114365369081497,
0.027532843872904778,
-0.0607922337949276,
-0.03680823743343353,
-0.03137844428420067,
0.15050069987773895,
0.06285841763019562,
-0.05876594036817551,
-0.02603764645755291,
-0.04899975657463074,
0.05127392336726189,
-0.062204450368881226,
-0.02417261153459549,
0.03146832063794136,
-0.04242103546857834,
0.07116831839084625,
-0.02575332298874855,
-0.02375098131597042,
0.027523165568709373,
-0.014608812518417835,
0.010071109980344772,
0.00284224352799356,
-0.05054609477519989,
-0.07812762260437012,
0.05685480311512947,
0.027842499315738678,
0.018151363357901573,
-0.01433282159268856,
-0.030952367931604385,
-0.05222979933023453,
-0.09731047600507736,
-0.023390257731080055,
0.02370546944439411,
-0.011001675389707088,
-0.01888754405081272,
0.017007822170853615,
-0.007655071094632149,
0.03061068430542946,
-0.010539771057665348,
0.028179816901683807,
-0.058835286647081375,
-0.05790921300649643,
-0.06002042442560196,
0.0790775939822197,
-0.03223279491066933,
-0.07479948550462723,
0.04339680075645447,
0.049547504633665085
] |
PMID:18543
|
Regulation of cell-mediated cytotoxicity. III. Synergism of cortico-resistant with anti-thymocyte serum-resistant splenic T cells in the generation of cytotoxic lymphocytes in graft-vs-host reactions.
|
Lethally irradiated F1 hybrid mice were given an i.v. injection of parental strain spleen cells. Six days later, their spleen cells were used as the effector cells to measure the in vitro cell-mediated cytotoxicity (CMC) of the parental cells. The treatment of the donors with hydrocortisone resulted in a marked decrease of the capacity of their spleen cells to produce a CMC reaction, whereas the treatment with antithymocyte serum (ATS) resulted in an almost complete loss of such activity. The mixing of spleen cells from hydrocortisone-treated parental donors with the spleen cells from ATS-treated parental donors before injection resulted in a synergistic amplification of the cytotoxic response. The anti-Thy-1 serum treatment of either spleen cell population abolished the synergism completely. These results indicate that cortico-resistant T cells act as precursors of cytotoxic lymphocytes and that ATS-resistant T cells produce an amplification of their reaction.
|
Regulation of cell-mediated cytotoxicity. III. Synergism of cortico-resistant with anti-thymocyte serum-resistant splenic T cells in the generation of cytotoxic lymphocytes in graft-vs-host reactions. Lethally irradiated F1 hybrid mice were given an i.v. injection of parental strain spleen cells. Six days later, their spleen cells were used as the effector cells to measure the in vitro cell-mediated cytotoxicity (CMC) of the parental cells. The treatment of the donors with hydrocortisone resulted in a marked decrease of the capacity of their spleen cells to produce a CMC reaction, whereas the treatment with antithymocyte serum (ATS) resulted in an almost complete loss of such activity. The mixing of spleen cells from hydrocortisone-treated parental donors with the spleen cells from ATS-treated parental donors before injection resulted in a synergistic amplification of the cytotoxic response. The anti-Thy-1 serum treatment of either spleen cell population abolished the synergism completely. These results indicate that cortico-resistant T cells act as precursors of cytotoxic lymphocytes and that ATS-resistant T cells produce an amplification of their reaction.
|
[
-0.06434805691242218,
0.028456293046474457,
-0.017731137573719025,
0.03604479879140854,
0.010246386751532555,
-0.017159949988126755,
0.08195587992668152,
0.06609516590833664,
-0.08036729693412781,
-0.01972765289247036,
0.04016004130244255,
-0.03190445899963379,
0.005917128175497055,
-0.03471573814749718,
-0.0001622500130906701,
0.006062319967895746,
-0.027629012241959572,
0.024247869849205017,
-0.061717212200164795,
0.03906744718551636,
-0.06348644942045212,
0.002098469529300928,
0.014965357258915901,
-0.0028674970380961895,
0.0053207967430353165,
-0.04427018016576767,
0.005302149336785078,
0.005546408239752054,
-0.04498010501265526,
-0.1810605674982071,
0.01950928010046482,
-0.024804232642054558,
-0.051809798926115036,
-0.013694697991013527,
-0.036209795624017715,
-0.03705815225839615,
0.0421096608042717,
-0.04211733117699623,
-0.013142921961843967,
0.06259632855653763,
0.01500687375664711,
0.0416686087846756,
0.023534854874014854,
0.08685395121574402,
0.01272587664425373,
-0.007807769346982241,
-0.004009325988590717,
-0.021120982244610786,
0.055170103907585144,
0.011494855396449566,
0.05706813931465149,
-0.05686401203274727,
0.009538072161376476,
0.049873098731040955,
-0.048398829996585846,
0.011564414016902447,
-0.017920760437846184,
0.07205953449010849,
0.06473975628614426,
0.06877011805772781,
-0.03585168719291687,
0.041537970304489136,
-0.1206379309296608,
0.07174401730298996,
0.056768983602523804,
0.03034304641187191,
-0.02922530472278595,
-0.0010061115026474,
0.041655998677015305,
0.05989290028810501,
-0.028341049328446388,
-0.001249929890036583,
-0.008337873965501785,
0.06664420664310455,
-0.009124478325247765,
0.032835640013217926,
-0.042864881455898285,
-0.03309358283877373,
0.014141998253762722,
0.009274749085307121,
-0.029304487630724907,
0.012823289260268211,
0.007127753458917141,
0.00036183412885293365,
-0.03312388435006142,
-0.0035623523872345686,
-0.034989651292562485,
-0.05911833792924881,
0.04331258684396744,
0.007547401823103428,
-0.02801053412258625,
-0.018229585140943527,
0.011446487158536911,
0.02851124480366707,
-0.052618756890296936,
-0.012661052867770195,
-0.006751219276338816,
-0.04639006778597832,
-0.007734015118330717,
0.4243929982185364,
-0.0011352451983839273,
0.029006846249103546,
-0.10745088011026382,
0.013762038201093674,
-0.01204877533018589,
-0.04752436652779579,
0.014475630596280098,
0.01264656987041235,
-0.022438690066337585,
0.04914760962128639,
-0.03352761268615723,
-0.03043654002249241,
-0.017225507646799088,
-0.017135249450802803,
-0.003635309636592865,
-0.03807765617966652,
0.07652103155851364,
-0.045727722346782684,
0.015571169555187225,
-0.010142049752175808,
0.04399572312831879,
-0.00423372583463788,
0.029146317392587662,
-0.04795239493250847,
0.06543233245611191,
-0.004965381231158972,
0.04025610163807869,
0.08737033605575562,
0.0615568645298481,
-0.02744016796350479,
0.05619274079799652,
0.0617588646709919,
-0.035477496683597565,
-0.011731710284948349,
0.0073157078586518764,
-0.005928274244070053,
-0.015367068350315094,
-0.07111986726522446,
0.005204301327466965,
0.01031713467091322,
0.053112126886844635,
-0.0876319482922554,
-0.001067106262780726,
-0.05110028758645058,
0.013282357715070248,
0.05808024853467941,
-0.00694864010438323,
-0.013011930510401726,
-0.008686712011694908,
0.0029482662212103605,
-0.030570950359106064,
0.0032706614583730698,
-0.005419377237558365,
0.048818036913871765,
-0.04747822880744934,
0.055620115250349045,
-0.05018307641148567,
0.025178618729114532,
0.04551958292722702,
-0.005135631188750267,
-0.01647057756781578,
-0.010101151652634144,
-0.03862212598323822,
0.07860071212053299,
0.03223340958356857,
-0.016339348629117012,
0.0235587190836668,
-0.0009331904584541917,
0.0202926117926836,
-0.017949149012565613,
0.00871417298913002,
-0.032842278480529785,
-0.023698195815086365,
-0.06485509127378464,
-0.08875628560781479,
0.03313892334699631,
-0.06336198002099991,
-0.005471257492899895,
0.02848859131336212,
0.01953844167292118,
0.021998267620801926,
-0.008596288040280342,
0.005243014544248581,
0.0777955949306488,
-0.025022419169545174,
0.010999966412782669,
-0.04647962376475334,
-0.016614757478237152,
-0.013991665095090866,
0.039994362741708755,
-0.0018083339091390371,
0.020401611924171448,
-0.077702596783638,
-0.02766892872750759,
-0.020348913967609406,
-0.03699599578976631,
-0.02449950762093067,
-0.028300713747739792,
-0.0002197234280174598,
-0.007804705761373043,
0.048425327986478806,
0.001478151767514646,
-0.020356914028525352,
0.054136257618665695,
-0.0011913885828107595,
0.008642558939754963,
0.0484277606010437,
0.005510426126420498,
0.08074577897787094,
-0.016484541818499565,
-0.06251919269561768,
0.008588219992816448,
0.00539665762335062,
0.03208416327834129,
-0.030057119205594063,
0.05787643790245056,
0.0151803744956851,
0.012540633790194988,
0.03397065028548241,
0.003945339471101761,
0.027692807838320732,
0.02329479157924652,
0.0183042511343956,
-0.3046441078186035,
-0.046401266008615494,
-0.05350297689437866,
-0.0006407001637853682,
-0.03926265612244606,
-0.007199856452643871,
-0.011304698884487152,
-0.06708760559558868,
-0.025768328458070755,
-0.05514884367585182,
0.08056385070085526,
0.1160784438252449,
-0.03969934955239296,
-0.08325373381376266,
-0.024357421323657036,
0.02626078575849533,
-0.04987166449427605,
-0.0007547530112788081,
0.08706153184175491,
0.03837571665644646,
-0.010726174339652061,
0.014456651173532009,
0.03704139590263367,
0.03340873494744301,
0.05149386450648308,
0.009404048323631287,
0.10547409951686859,
0.013013012707233429,
-0.024302683770656586,
0.008823323994874954,
0.044210467487573624,
0.03307696431875229,
0.038789115846157074,
-0.03769049048423767,
-0.004395044408738613,
-0.021872660145163536,
-0.012769612483680248,
-0.03140930086374283,
0.010407387278974056,
0.011261950246989727,
-0.040584176778793335,
-0.006244482938200235,
-0.02896939031779766,
-0.11145959049463272,
-0.04320627078413963,
0.033800411969423294,
0.011264058761298656,
0.0882202535867691,
0.0016536896582692862,
-0.019618485122919083,
-0.01441073790192604,
-0.006471916567534208,
0.023282697424292564,
0.015828987583518028,
0.05538800358772278,
0.022734908387064934,
-0.05822961777448654,
-0.009080425836145878,
-0.08744047582149506,
-0.04652518406510353,
-0.04725785553455353,
0.017603224143385887,
0.008922562934458256,
-0.020516695454716682,
-0.00998197216540575,
-0.022601651027798653,
0.04616578668355942,
-0.04067785665392876,
0.045329056680202484,
0.029575495049357414,
0.013347556814551353,
0.10532153397798538,
0.01766139641404152,
0.016100481152534485,
-0.002625579945743084,
0.05905569717288017,
-0.04988844692707062,
-0.02387215569615364,
-0.10863759368658066,
0.003286454128101468,
-0.012716881930828094,
-0.011510143987834454,
0.020058175548911095,
-0.03280866891145706,
0.0030437547247856855,
0.012029379606246948,
0.012908343225717545,
0.0038384003564715385,
-0.04573424905538559,
-0.02884683758020401,
-0.029378917068243027,
0.0014512503985315561,
-0.02372528798878193,
-0.03486353158950806,
-0.006279314868152142,
0.02669927105307579,
-0.2790435254573822,
-0.013870333321392536,
0.023125259205698967,
-0.011184045113623142,
-0.028864305466413498,
-0.021617256104946136,
0.04671229049563408,
-0.005892863497138023,
-0.018260711804032326,
0.08057822287082672,
0.1107112243771553,
0.02410835586488247,
0.04909524694085121,
0.07243847101926804,
0.008180678822100163,
-0.02322414331138134,
0.030865252017974854,
-0.05548477917909622,
-0.004554027691483498,
-0.024615854024887085,
0.051711440086364746,
0.004332344979047775,
0.11546366661787033,
-0.017086956650018692,
0.009157209657132626,
0.02969178557395935,
-0.0041693816892802715,
0.06364591419696808,
-0.019721124321222305,
0.014837651513516903,
0.031515587121248245,
-0.011128961108624935,
-0.0017449144506826997,
0.013992547988891602,
0.049500394612550735,
0.03284817934036255,
-0.002558486768975854,
0.01557422336190939,
0.0033841822296380997,
0.01594652608036995,
-0.02682737447321415,
0.04910934343934059,
-0.05878503993153572,
-0.05218452215194702,
0.10609763860702515,
-0.0029821970965713263,
-0.006033024284988642,
-0.04427524656057358,
-0.029841329902410507,
-0.01475825160741806,
-0.0330534353852272,
-0.011777200736105442,
0.04016414284706116,
-0.03147490695118904,
-0.030844364315271378,
-0.031809307634830475,
-0.04358277469873428,
-0.02712978608906269,
0.01645541936159134,
0.036699697375297546,
0.030743079259991646,
-0.02632053568959236,
-0.011190973222255707,
0.07859067618846893,
0.0032839218620210886
] |
PMID:18546
|
Differential susceptibility of Escherichia coli and Proteus mirabilis to mouse urine and to urea.
|
The purpose of this investigation was to compare the antimicrobial properties of mouse urine and of urea against Escherichia coli and Proteus mirabilis. Nornal urine was found to inhibit the growth of E. coli and P. mirabilis, whereas urine from diuresing animals permitted multiplication of these bacteria. Addition of urea to urine from diuresing animals restored its bactericidal effect on P. mirabilis but not on E. coli. This bactericidal effect on P. mirabilis was dependent on the additive action of high content of urea and high pH and was prevented by the addition of urease inhibitor.
|
Differential susceptibility of Escherichia coli and Proteus mirabilis to mouse urine and to urea. The purpose of this investigation was to compare the antimicrobial properties of mouse urine and of urea against Escherichia coli and Proteus mirabilis. Nornal urine was found to inhibit the growth of E. coli and P. mirabilis, whereas urine from diuresing animals permitted multiplication of these bacteria. Addition of urea to urine from diuresing animals restored its bactericidal effect on P. mirabilis but not on E. coli. This bactericidal effect on P. mirabilis was dependent on the additive action of high content of urea and high pH and was prevented by the addition of urease inhibitor.
|
[
-0.03218415006995201,
-0.025633106008172035,
0.048161156475543976,
0.043373968452215195,
0.05621466785669327,
-0.03957395255565643,
0.05858272314071655,
0.08383200317621231,
-0.04650130122900009,
0.02509000152349472,
-0.015596816316246986,
-0.044945891946554184,
-0.02570492960512638,
0.006863495800644159,
-0.024738578125834465,
-0.02643759734928608,
-0.0462694875895977,
0.006002913229167461,
-0.08838847279548645,
0.02562347985804081,
0.09236199408769608,
0.010902605019509792,
0.013254941441118717,
-0.0348801426589489,
0.011062653735280037,
-0.03821733966469765,
0.02847493626177311,
0.006886330433189869,
-0.07646038383245468,
-0.17732971906661987,
0.0030624070204794407,
-0.04183574765920639,
0.0014871637104079127,
-0.02739744447171688,
-0.037944406270980835,
0.02286107838153839,
-0.07490422576665878,
-0.026571664959192276,
-0.039439182728528976,
-0.003733268240466714,
0.0040804548189044,
0.05792208015918732,
0.0362943634390831,
-0.002074587857350707,
0.016351329162716866,
0.002840050496160984,
-0.026041964069008827,
0.0059024132788181305,
0.12582501769065857,
0.02302582934498787,
0.04136556759476662,
-0.031779177486896515,
0.041657160967588425,
0.03679070994257927,
0.008272552862763405,
-0.04521499201655388,
0.04685182869434357,
-0.015056610107421875,
-0.02958395890891552,
0.043752431869506836,
-0.008992657996714115,
-0.014296730048954487,
-0.09953214973211288,
0.02776479348540306,
0.031272899359464645,
-0.02971847914159298,
0.005628351587802172,
0.03271223232150078,
0.0651509240269661,
-0.01475643552839756,
-0.02005182020366192,
0.027982814237475395,
-0.06734885275363922,
0.052046243101358414,
0.010892731137573719,
0.045275792479515076,
0.02328336238861084,
0.030016951262950897,
0.061010412871837616,
0.0661705732345581,
-0.04432138055562973,
0.007682656403630972,
0.038614243268966675,
-0.023224569857120514,
-0.01325204037129879,
-0.04618104547262192,
-0.0204296987503767,
0.010289086028933525,
0.030213026329874992,
0.013427800498902798,
-0.02976369857788086,
-0.00874902680516243,
-0.01215419266372919,
-0.026638220995664597,
-0.013696497306227684,
-0.04569166153669357,
0.003699489636346698,
-0.09072601795196533,
0.01839364506304264,
0.3575679659843445,
-0.06890515238046646,
-0.028764991089701653,
-0.012175394222140312,
-0.03249030187726021,
0.0880146324634552,
0.0313425213098526,
0.03155292570590973,
-0.004839979112148285,
0.029414456337690353,
0.07317106425762177,
0.03995949402451515,
0.03556773439049721,
-0.03207291290163994,
0.004118200857192278,
0.006166728213429451,
0.04591359570622444,
0.031448185443878174,
-0.007380579132586718,
-0.017218997702002525,
-0.005723073147237301,
0.0022237070370465517,
-0.04689367860555649,
0.04722125455737114,
-0.040946733206510544,
-0.03191293403506279,
-0.051995649933815,
-0.004102305043488741,
0.06661314517259598,
-0.03002558834850788,
0.0067904032766819,
-0.011944287456572056,
-0.031118080019950867,
0.0023082310799509287,
-0.0120132677257061,
0.021905090659856796,
-0.014371290802955627,
0.041998233646154404,
-0.06261622905731201,
0.0383637472987175,
0.014087734743952751,
-0.09137663245201111,
-0.08945110440254211,
-0.055873554199934006,
-0.08524686843156815,
-0.051939718425273895,
0.055101051926612854,
0.014879043214023113,
0.03363760560750961,
-0.017046188935637474,
0.01074124500155449,
0.020584626123309135,
0.09290187060832977,
0.006303416099399328,
-0.013846228830516338,
0.0053610182367265224,
0.06296274065971375,
0.03936884552240372,
-0.008287757635116577,
0.03608061745762825,
-0.06498000025749207,
-0.0523063950240612,
-0.019206812605261803,
-0.01613757200539112,
0.04544319957494736,
0.006783200427889824,
0.048306625336408615,
-0.022822799161076546,
0.013692566193640232,
0.0013995590852573514,
0.04729011654853821,
0.018823102116584778,
-0.007519140839576721,
-0.015413600020110607,
-0.015316145494580269,
-0.08854468166828156,
-0.0027893027290701866,
-0.05276258662343025,
-0.0013958434574306011,
0.053239572793245316,
0.023119555786252022,
-0.021207252517342567,
-0.021443897858262062,
-0.02405727095901966,
0.020678885281085968,
-0.000462928059278056,
-0.013037294149398804,
-0.055585406720638275,
0.031261131167411804,
0.027363847941160202,
0.07763717323541641,
0.04715356230735779,
-0.062799371778965,
-0.07093255966901779,
-0.031546127051115036,
0.024935629218816757,
0.015975110232830048,
-0.06027153506875038,
0.02233734168112278,
-0.02164909988641739,
0.023798596113920212,
0.09198068082332611,
0.001816126867197454,
-0.024931078776717186,
-0.04068342223763466,
-0.038946688175201416,
0.02488791011273861,
0.05225850269198418,
0.02304469235241413,
-0.041322771459817886,
-0.035861529409885406,
-0.06834491342306137,
-0.008269351907074451,
-0.06480912119150162,
-0.027499869465827942,
-0.020997513085603714,
0.06189977750182152,
0.04795772582292557,
0.002115431008860469,
0.11045343428850174,
0.05497874319553375,
0.08858548104763031,
-0.05078321322798729,
-0.022009162232279778,
-0.3068676292896271,
-0.028512611985206604,
-0.020947188138961792,
-0.0495939701795578,
0.0520104356110096,
-0.05706988275051117,
-0.00026203112793155015,
-0.012676408514380455,
-0.051813509315252304,
-0.01514481846243143,
0.07936039566993713,
0.044951651245355606,
-0.008947938680648804,
0.020285990089178085,
-0.05687659606337547,
0.04157243296504021,
0.028781957924365997,
-0.022863900288939476,
0.016138268634676933,
-0.008906119503080845,
0.001517711440101266,
0.021756010130047798,
0.06556767970323563,
0.03985504433512688,
0.029173647984862328,
-0.017505448311567307,
0.10578325390815735,
0.01209547370672226,
0.020740043371915817,
-0.020955324172973633,
0.0032492605969309807,
0.0360131561756134,
-0.02531580999493599,
-0.16037534177303314,
-0.0272726658731699,
0.04607371985912323,
-0.03756990656256676,
-0.07478442788124084,
0.0036620600149035454,
-0.035085760056972504,
-0.0400032214820385,
0.04269903525710106,
-0.010032941587269306,
-0.0658126100897789,
-0.06345781683921814,
-0.023632841184735298,
0.05168167129158974,
0.056068357080221176,
0.024939248338341713,
0.06069144234061241,
-0.006612640805542469,
0.0674409419298172,
0.00986915361136198,
0.010693293064832687,
-0.008764684200286865,
0.0029971362091600895,
-0.0067710550501942635,
0.01076993253082037,
0.00893192645162344,
-0.0484609380364418,
-0.014638307504355907,
0.004365105647593737,
0.04502183571457863,
-0.0019566090777516365,
0.007523985579609871,
-0.04241331294178963,
-0.028247851878404617,
-0.00932326354086399,
0.08410592377185822,
0.056344836950302124,
0.008106308989226818,
0.11824923008680344,
0.0021661759819835424,
0.051603395491838455,
-0.009644681587815285,
0.047726601362228394,
-0.01950620673596859,
-0.046560999006032944,
-0.007099888753145933,
0.00856783241033554,
0.017502708360552788,
-0.004908828996121883,
0.051127903163433075,
-0.052400216460227966,
0.0360163114964962,
0.011596144177019596,
0.0039843106642365456,
-0.010908604599535465,
0.030153214931488037,
0.022779351100325584,
0.02976512350142002,
-0.01828729547560215,
-0.017742367461323738,
-0.023616433143615723,
-0.007973229512572289,
0.005890429951250553,
-0.2844969928264618,
-0.008452541194856167,
0.053000643849372864,
-0.03969880938529968,
0.013363135047256947,
0.027660854160785675,
-0.004179456736892462,
-0.06532325595617294,
-0.011637588031589985,
0.018425308167934418,
0.05222237855195999,
0.002728302264586091,
-0.01205497793853283,
0.018241114914417267,
0.019201135262846947,
-0.0006820139242336154,
0.046481654047966,
0.028135214000940323,
0.030570408329367638,
-0.005134792532771826,
0.04753066971898079,
-0.05600627511739731,
0.18081629276275635,
-0.022741269320249557,
0.01177898421883583,
0.04235316812992096,
0.018474936485290527,
0.031359512358903885,
-0.04422266036272049,
0.006647948175668716,
-0.006466838996857405,
0.009816396981477737,
0.02959107607603073,
-0.03931437060236931,
0.0049767643213272095,
0.019819803535938263,
0.0034020585007965565,
0.01509721577167511,
0.019798677414655685,
-0.06290709227323532,
-0.06451044976711273,
0.0024026583414524794,
-0.09197413921356201,
-0.05052242428064346,
0.08352047950029373,
-0.036699388176202774,
0.008152030408382416,
-0.06401674449443817,
0.014057478867471218,
-0.0323278047144413,
-0.013876911252737045,
-0.009955592453479767,
0.01690402254462242,
0.023960478603839874,
0.03143602982163429,
-0.029981138184666634,
0.0004198618698865175,
-0.03305569663643837,
-0.036657996475696564,
0.0005778970662504435,
0.04340359941124916,
-0.000335890450514853,
-0.03258819133043289,
0.05258241295814514,
0.013957572169601917
] |
PMID:18549
|
Alteration of structure and penetrability of the vitelline envelope after passage of eggs from coelom to oviduct in Xenopus laevis.
|
The vitelline envelope (VE) that surrounds an egg released from the ovary into the coelom of Xenopus laevis differs markedly, in structure and penetrability, from the VE surrounding an oviposited egg. In a coelomic egg, the filaments that form the VE are arranged in distinct fascicles or bundles. The exterior surface of the VE is irregular in contour and is permeated by channels. In an oviposited egg, the filaments are evenly dispersed and lack a fasciculated arrangement; the exterior surface is smooth and no channels are present. The fascicular arrangement of fibrils in the coelomic VE is maintained only at neutral pH, and is not visibly altered by the cortical reaction. VEs from coelomic eggs retain their fasciculated morphology after isolation from the egg. In an in vitro test system, sperm penetrated VEs isolated from oviposited eggs, but failed to penetrate VEs isolated from coelomic eggs. The structural transformation of the VE from the coelomic type to the oviposited type occurs in the first 1-cm segment of the oviduct, prior to addition of jelly to the egg. Neither intact jelly, solubilized jelly, nor jelly extracts were capable of altering the structural organization of coelomic VEs, suggesting that the structural transformation of the VE is effected by some oviducal factor other than jelly.
|
Alteration of structure and penetrability of the vitelline envelope after passage of eggs from coelom to oviduct in Xenopus laevis. The vitelline envelope (VE) that surrounds an egg released from the ovary into the coelom of Xenopus laevis differs markedly, in structure and penetrability, from the VE surrounding an oviposited egg. In a coelomic egg, the filaments that form the VE are arranged in distinct fascicles or bundles. The exterior surface of the VE is irregular in contour and is permeated by channels. In an oviposited egg, the filaments are evenly dispersed and lack a fasciculated arrangement; the exterior surface is smooth and no channels are present. The fascicular arrangement of fibrils in the coelomic VE is maintained only at neutral pH, and is not visibly altered by the cortical reaction. VEs from coelomic eggs retain their fasciculated morphology after isolation from the egg. In an in vitro test system, sperm penetrated VEs isolated from oviposited eggs, but failed to penetrate VEs isolated from coelomic eggs. The structural transformation of the VE from the coelomic type to the oviposited type occurs in the first 1-cm segment of the oviduct, prior to addition of jelly to the egg. Neither intact jelly, solubilized jelly, nor jelly extracts were capable of altering the structural organization of coelomic VEs, suggesting that the structural transformation of the VE is effected by some oviducal factor other than jelly.
|
[
-0.008636374957859516,
-0.05452967807650566,
0.00962029304355383,
0.03635752573609352,
-0.03178250044584274,
0.04943361133337021,
0.004576397128403187,
0.044574178755283356,
-0.017182128503918648,
0.0030889068730175495,
-0.011536547914147377,
-0.0859702080488205,
0.00600108178332448,
-0.015154309570789337,
0.00009436847903998569,
-0.022332651540637016,
-0.06203872337937355,
-0.0021135308779776096,
-0.08384191244840622,
0.03223993629217148,
0.03475825861096382,
-0.00894064363092184,
0.015061079524457455,
-0.04874828830361366,
0.01720312424004078,
0.03126956522464752,
0.005930516868829727,
0.02012658677995205,
-0.02218395285308361,
-0.22728292644023895,
-0.0037493191193789244,
0.00005948411126155406,
-0.02349737659096718,
-0.04528188332915306,
-0.047165706753730774,
0.02298077940940857,
-0.01889561302959919,
-0.02845514938235283,
-0.010627536103129387,
0.028808441013097763,
0.04496822506189346,
0.020930752158164978,
0.007868076674640179,
-0.050685059279203415,
0.0032917512580752373,
-0.02274860441684723,
0.007341994903981686,
0.0005639009759761393,
0.060419995337724686,
-0.008478783071041107,
0.0030045334715396166,
-0.0007481236825697124,
0.009289871901273727,
0.0382855124771595,
-0.010244462639093399,
0.060052741318941116,
-0.010949921794235706,
0.007590331602841616,
0.021292373538017273,
-0.01316501758992672,
-0.028872013092041016,
0.05335108935832977,
-0.10272549092769623,
0.08593074977397919,
0.065945103764534,
0.03935664892196655,
-0.002594543853774667,
0.02159741334617138,
0.049838438630104065,
0.009755552746355534,
-0.03806972876191139,
0.012895764783024788,
-0.021675266325473785,
-0.04748031124472618,
0.04152575135231018,
0.006508239079266787,
0.026068469509482384,
0.010121439583599567,
0.007533062249422073,
0.012582104653120041,
-0.03921588510274887,
0.008458039723336697,
0.012686733156442642,
0.02369776926934719,
-0.01919197291135788,
0.050886668264865875,
0.02023322880268097,
-0.06966845691204071,
-0.0007441808120347559,
-0.009792986325919628,
-0.034826431423425674,
-0.05974992737174034,
0.009522133506834507,
-0.012220093049108982,
-0.12718410789966583,
0.038808729499578476,
0.005852153990417719,
-0.07339052855968475,
0.05088040605187416,
0.3685759902000427,
-0.028767742216587067,
0.02685275487601757,
-0.002756407018750906,
0.008473832160234451,
0.03413010761141777,
-0.04629015550017357,
-0.018780667334794998,
-0.026763008907437325,
0.00204957602545619,
0.1129951924085617,
0.02339220605790615,
0.0035832026042044163,
-0.003032195381820202,
-0.03269747644662857,
-0.023103052750229836,
-0.048791948705911636,
-0.014579279348254204,
0.003471564734354615,
-0.06586780399084091,
0.04776421934366226,
-0.03369549289345741,
0.043722931295633316,
-0.0074890898540616035,
-0.014877730049192905,
0.0340302549302578,
0.038768794387578964,
0.00745855737477541,
0.059449248015880585,
0.02917637676000595,
0.011446419171988964,
0.019349845126271248,
0.10277233272790909,
-0.0001707633346086368,
-0.014922874048352242,
-0.00952807068824768,
0.04354364797472954,
0.0010383992921561003,
-0.04324401915073395,
-0.0295871589332819,
0.014111134223639965,
-0.01487352792173624,
-0.07502878457307816,
0.008733958937227726,
-0.04000183939933777,
-0.008581273257732391,
0.07072336971759796,
-0.01360649149864912,
0.03391142189502716,
0.010013716295361519,
0.035668231546878815,
-0.004129732958972454,
0.07075436413288116,
-0.06932427734136581,
0.02263808064162731,
-0.00243347673676908,
0.014787035062909126,
-0.05363308638334274,
0.046245116740465164,
-0.014572388492524624,
-0.0014360676286742091,
-0.022279970347881317,
0.0022330477368086576,
-0.029089899733662605,
0.08885345607995987,
0.031712256371974945,
-0.05061362311244011,
0.011071042157709599,
0.04059005156159401,
0.02511698007583618,
-0.05291704088449478,
-0.001862952602095902,
0.017378363758325577,
0.029110610485076904,
-0.04579378664493561,
-0.006152857560664415,
0.024052048102021217,
-0.04562467336654663,
-0.0419771745800972,
0.02406383492052555,
0.01897551864385605,
0.025707362219691277,
-0.054438021034002304,
-0.027262095361948013,
0.02037018910050392,
0.035858508199453354,
-0.03323337808251381,
-0.08025713264942169,
0.023766689002513885,
0.034532658755779266,
0.03230034559965134,
-0.033821623772382736,
-0.02131589502096176,
-0.05570094287395477,
0.04896422103047371,
-0.07545509934425354,
0.023023860529065132,
0.0008384950924664736,
0.043733660131692886,
-0.01997644267976284,
-0.024214409291744232,
0.12106474488973618,
-0.025378823280334473,
0.015345548279583454,
0.07264376431703568,
-0.010462789796292782,
-0.014022143557667732,
0.08445467799901962,
-0.05322834476828575,
0.015298894606530666,
0.03149061277508736,
-0.04832073673605919,
-0.03840135410428047,
-0.025103384628891945,
0.03817247226834297,
-0.007695959415286779,
0.06058109924197197,
0.0009760406101122499,
-0.07621093094348907,
0.09850389510393143,
-0.014292318373918533,
0.0371515192091465,
-0.0676741898059845,
-0.02752966806292534,
-0.3234387934207916,
-0.05052771791815758,
-0.0014450072776526213,
0.004327680915594101,
0.05178029090166092,
-0.053146105259656906,
0.03818376734852791,
-0.010157429613173008,
0.055114660412073135,
-0.11822650581598282,
0.025769157335162163,
0.04559600353240967,
-0.001235664589330554,
-0.012407703325152397,
0.034552570432424545,
0.04110089689493179,
0.07348629832267761,
-0.022114358842372894,
-0.01620304211974144,
0.0011482873233035207,
-0.06718160957098007,
-0.05109899491071701,
0.0135434465482831,
0.056652747094631195,
0.03346457704901695,
-0.010035370476543903,
0.1525978147983551,
0.024566572159528732,
0.0021419490221887827,
-0.0276927687227726,
-0.016551129519939423,
0.04823977127671242,
-0.029772985726594925,
-0.04511645808815956,
0.030211295932531357,
0.021993229165673256,
0.020602324977517128,
-0.02221013978123665,
-0.009518713690340519,
0.004396697971969843,
-0.035547517240047455,
0.022232774645090103,
-0.007593092974275351,
-0.05637035518884659,
-0.0257027018815279,
0.044702641665935516,
-0.007908163592219353,
0.04896662011742592,
-0.02185545489192009,
0.0032367112580686808,
0.023947102949023247,
-0.01300372276455164,
0.057425606995821,
0.022647805511951447,
0.012900800444185734,
0.012427696026861668,
-0.05183007940649986,
-0.0014617436099797487,
-0.02154584228992462,
0.010024858638644218,
0.005716435611248016,
0.0556231252849102,
-0.04343855381011963,
-0.05546543374657631,
-0.01611241325736046,
0.028757967054843903,
0.0053261821158230305,
-0.03874635696411133,
0.046075303107500076,
-0.033253807574510574,
0.004503609146922827,
0.05460190400481224,
-0.07199936360120773,
-0.027115968987345695,
-0.004559619352221489,
-0.0007153366459533572,
-0.001798768062144518,
-0.025136673822999,
-0.029102956876158714,
0.01478126086294651,
0.025768736377358437,
-0.05828675627708435,
-0.00846155732870102,
-0.006329594645649195,
-0.003057988826185465,
0.04135546460747719,
-0.026679852977395058,
-0.048389509320259094,
0.027230748906731606,
0.05071113258600235,
-0.013357345014810562,
-0.012658628635108471,
-0.021547801792621613,
0.027312275022268295,
0.011745957657694817,
0.040592871606349945,
-0.29360753297805786,
0.024251652881503105,
0.05291331186890602,
0.030083706602454185,
0.0026656535919755697,
0.03309197351336479,
0.017141859978437424,
-0.027736375108361244,
-0.01788034476339817,
0.061402153223752975,
-0.03294754400849342,
-0.008177871815860271,
0.040378060191869736,
0.07539989799261093,
-0.026932120323181152,
0.08870391547679901,
0.06008254364132881,
-0.05126762390136719,
0.0198539886623621,
-0.01425399910658598,
-0.015135607682168484,
-0.0157296322286129,
0.1884666532278061,
0.0038871525321155787,
-0.0020255660638213158,
-0.04720127210021019,
-0.006853930186480284,
0.07303223758935928,
-0.02057977393269539,
-0.01686999946832657,
-0.01416376605629921,
0.0016040154732763767,
0.009942527860403061,
-0.01432119868695736,
-0.01725587248802185,
-0.020413342863321304,
0.03289148211479187,
-0.024791043251752853,
0.036864425987005234,
-0.007165316492319107,
0.03516177460551262,
-0.007783136796206236,
-0.08281461149454117,
-0.013038809411227703,
0.024431956931948662,
-0.06472306698560715,
-0.015391199849545956,
0.013682914897799492,
0.029115354642271996,
0.0005434199701994658,
0.014737753197550774,
-0.056208424270153046,
-0.03156409412622452,
0.007129669189453125,
-0.04126429930329323,
-0.051934897899627686,
0.006398944184184074,
-0.007654896471649408,
-0.060497742146253586,
0.06207728385925293,
0.022102979943156242,
-0.04727713391184807,
-0.005999608896672726,
0.08801164478063583,
0.07272637635469437
] |
PMID:18550
|
Ribonucleotide reductase from herpes simplex virus (types 1 and 2) infected and uninfected KB cells: properties of the partially purified enzymes.
|
Mammalian ribonucleotide reductase is a complex enzyme modified in its activity by a complex regulatory system involving adenosine triphosphate (ATP) and deoxyribonucleoside triphosphates. Infection of KB cells with herpes simplex virus (HSV) type 1 or 2 induces the formation of an altered ribonucleotide reductase. The properties of partially purified reductase from uninfected KB cells have been compared with the enzymes obtained from HSV-1 and HSV-2 infected KB cells. We found that the virus-induced enzymes are similar to the KB enzyme in some properties but differed significantly from the host enzyme in three respects: (1) virus induced reductase was not inhibited significantly by deoxythymidine triphosphate regardless of ATP concentration, (2) magnesium was not required for virus enzyme activity although 2 mM-Mg2+ did stimulate the reaction, and (3) magnesium concentration required for optimal activity was different for virus and host enzymes. These changes are evidence that the enzyme molecules present after infection by HSV-1 or HSV-2 differ from those present before infection.
|
Ribonucleotide reductase from herpes simplex virus (types 1 and 2) infected and uninfected KB cells: properties of the partially purified enzymes. Mammalian ribonucleotide reductase is a complex enzyme modified in its activity by a complex regulatory system involving adenosine triphosphate (ATP) and deoxyribonucleoside triphosphates. Infection of KB cells with herpes simplex virus (HSV) type 1 or 2 induces the formation of an altered ribonucleotide reductase. The properties of partially purified reductase from uninfected KB cells have been compared with the enzymes obtained from HSV-1 and HSV-2 infected KB cells. We found that the virus-induced enzymes are similar to the KB enzyme in some properties but differed significantly from the host enzyme in three respects: (1) virus induced reductase was not inhibited significantly by deoxythymidine triphosphate regardless of ATP concentration, (2) magnesium was not required for virus enzyme activity although 2 mM-Mg2+ did stimulate the reaction, and (3) magnesium concentration required for optimal activity was different for virus and host enzymes. These changes are evidence that the enzyme molecules present after infection by HSV-1 or HSV-2 differ from those present before infection.
|
[
-0.05809590592980385,
-0.004608899354934692,
0.011007243767380714,
0.007673701737076044,
0.04502755030989647,
-0.05402596667408943,
0.05028440058231354,
0.061360977590084076,
-0.04418984428048134,
-0.008749050088226795,
0.02738015539944172,
-0.07212725281715393,
0.03761812299489975,
-0.03762587904930115,
-0.01226377859711647,
-0.054848190397024155,
0.014480714686214924,
-0.003623899072408676,
-0.08591609448194504,
0.004708833992481232,
-0.08032490313053131,
-0.00025348938652314246,
-0.03316309303045273,
-0.028649505227804184,
0.020971713587641716,
0.0014222324825823307,
0.044397901743650436,
0.023085055872797966,
-0.061716075986623764,
-0.17216508090496063,
0.03026501089334488,
-0.04832364246249199,
0.01371911633759737,
-0.0739678144454956,
0.04848398268222809,
0.0034934275317937136,
-0.044069621711969376,
-0.04218141362071037,
-0.00950508564710617,
0.02195870317518711,
0.07269860059022903,
0.006789830047637224,
0.09791535139083862,
0.015751292929053307,
-0.018282771110534668,
0.0032685454934835434,
-0.0767337754368782,
0.017607608810067177,
0.10182805359363556,
-0.008240943774580956,
-0.011895722709596157,
0.022544391453266144,
0.012678108178079128,
0.017525427043437958,
0.04925551638007164,
-0.01023069117218256,
0.05265957862138748,
0.0554521270096302,
-0.055926285684108734,
0.0008557983674108982,
0.022595517337322235,
0.02938925474882126,
-0.10725127905607224,
0.04513245448470116,
0.05159727483987808,
-0.0329877994954586,
0.020731911063194275,
-0.0829230323433876,
0.03372608497738838,
0.03858691453933716,
-0.08670879900455475,
0.01878875121474266,
-0.021708950400352478,
0.021785829216241837,
0.0003145496884826571,
-0.029296278953552246,
-0.053099751472473145,
0.05113556981086731,
0.0655428022146225,
0.0005781611544080079,
-0.013694905675947666,
0.0025532119907438755,
0.01958819478750229,
0.0033226811792701483,
-0.015024533495306969,
0.011641191318631172,
0.010960908606648445,
-0.016789399087429047,
0.04047476872801781,
0.01198359951376915,
-0.005448340438306332,
0.010770195163786411,
0.024639392271637917,
-0.026250150054693222,
-0.06948117166757584,
0.09262555092573166,
0.04577634111046791,
0.009028058499097824,
0.06051832064986229,
0.36728933453559875,
-0.034846119582653046,
0.013621514663100243,
-0.09483184665441513,
-0.028005331754684448,
-0.03211796283721924,
-0.06558886915445328,
0.031244246289134026,
-0.0049447184428572655,
-0.02279907651245594,
0.052225176244974136,
0.06424775719642639,
-0.05016786977648735,
-0.03732321038842201,
-0.013091281987726688,
-0.00027716992190107703,
0.034353576600551605,
-0.029170477762818336,
0.07295212149620056,
-0.037784092128276825,
0.08179548382759094,
-0.03652846813201904,
-0.025505375117063522,
0.05716213956475258,
-0.025779377669095993,
0.06050095707178116,
0.03952204808592796,
-0.014474514871835709,
0.10941904783248901,
0.08245775103569031,
0.022052479907870293,
0.048271603882312775,
0.0275392048060894,
-0.02261126972734928,
0.0041201068088412285,
0.018064109608530998,
0.015639767050743103,
-0.013037470169365406,
0.022040145471692085,
0.06314690411090851,
-0.034706633538007736,
0.014729238115251064,
-0.013398952782154083,
-0.09190621227025986,
-0.06444071233272552,
-0.021465269848704338,
0.01689521223306656,
-0.03481258451938629,
0.08857344835996628,
0.004993991926312447,
-0.02212231047451496,
0.03475183621048927,
0.09425932168960571,
-0.015108480118215084,
-0.010250620543956757,
-0.02418256178498268,
-0.026606103405356407,
-0.006462561897933483,
-0.06775739789009094,
0.017421821132302284,
-0.024600917473435402,
-0.06091694161295891,
-0.016398560255765915,
0.04905347526073456,
0.06742719560861588,
-0.0047984556294977665,
-0.020437857136130333,
-0.015667682513594627,
0.020189305767416954,
0.06440552324056625,
0.0029827828984707594,
0.020497607067227364,
0.012383557856082916,
-0.05241832137107849,
-0.01976155862212181,
-0.03817819803953171,
-0.02692146971821785,
-0.02563694678246975,
0.04901749640703201,
0.03390742465853691,
0.008154930546879768,
0.020120637491345406,
-0.04325171560049057,
-0.08674593269824982,
0.02777988463640213,
0.038960281759500504,
-0.008218261413276196,
-0.0338347889482975,
0.07018454372882843,
0.04280422255396843,
0.04439355432987213,
-0.035185556858778,
0.01768128015100956,
-0.07572602480649948,
-0.05422040447592735,
-0.0006669489084742963,
-0.010858834721148014,
0.0006905964692123234,
0.012867777608335018,
-0.010507817380130291,
-0.010929970070719719,
0.08999068289995193,
0.020436802878975868,
0.012425929307937622,
0.07972118258476257,
-0.07597800344228745,
0.011796549893915653,
-0.006574091035872698,
0.06184261292219162,
0.01723393425345421,
0.001585782621987164,
-0.012203425168991089,
0.003187038004398346,
-0.09599708765745163,
0.0048589580692350864,
-0.06773553788661957,
0.0205282811075449,
-0.024548880755901337,
0.0037398594431579113,
0.05152592435479164,
0.00709720142185688,
0.013919282704591751,
-0.03806057944893837,
-0.016419000923633575,
-0.2951854467391968,
-0.04036394879221916,
-0.011357184499502182,
-0.01572943851351738,
0.014364635571837425,
-0.00040617780177854,
0.0016093540471047163,
-0.0042972625233232975,
0.002405584789812565,
0.01297102589160204,
0.013509788550436497,
0.02727929875254631,
-0.01677171140909195,
-0.09877654910087585,
-0.017135418951511383,
0.051923856139183044,
-0.01506140548735857,
-0.08375000208616257,
0.01687103696167469,
-0.01993853785097599,
0.04514079913496971,
-0.011160112917423248,
0.011794333346188068,
-0.013982982374727726,
0.07408324629068375,
-0.0020097605884075165,
0.0971660166978836,
0.04097551107406616,
0.08368971198797226,
0.007717899978160858,
0.0032395159360021353,
0.05011134594678879,
-0.033545009791851044,
-0.09425423294305801,
-0.010104849003255367,
0.006569060031324625,
-0.03634972125291824,
-0.008760090917348862,
-0.011019608937203884,
-0.029479997232556343,
0.008555639535188675,
0.0035075778141617775,
-0.004495161585509777,
0.009537746198475361,
0.06558066606521606,
-0.0020973533391952515,
0.002597431419417262,
0.014909833669662476,
0.047389157116413116,
0.0028603810351341963,
0.0217424463480711,
0.011841466650366783,
0.010941824875772,
0.0334462933242321,
0.013013532385230064,
-0.034507185220718384,
-0.06591431796550751,
0.008641762658953667,
-0.02378655970096588,
-0.010721278376877308,
-0.0005429192096926272,
-0.044200412929058075,
-0.02932315319776535,
0.01846187189221382,
-0.07095622271299362,
-0.024367395788431168,
-0.02017073705792427,
-0.016324812546372414,
0.05502048507332802,
0.0003194208547938615,
-0.04271490126848221,
0.10894372314214706,
-0.017306959256529808,
0.00964016281068325,
0.030690712854266167,
0.03249112889170647,
-0.037138331681489944,
-0.02695879153907299,
-0.06136348471045494,
0.03399735316634178,
0.018383299931883812,
-0.020547058433294296,
0.10332545638084412,
0.008670175448060036,
-0.007445978466421366,
-0.028048507869243622,
-0.011563935317099094,
0.030160872265696526,
0.010792833752930164,
0.0008128277841024101,
0.04914969205856323,
-0.07365050166845322,
-0.014327417127788067,
-0.0041946363635361195,
0.02187022939324379,
-0.026434699073433876,
-0.24159416556358337,
-0.011716650798916817,
-0.011755727231502533,
-0.010590402409434319,
-0.065769724547863,
0.027545645833015442,
-0.008527889847755432,
-0.05646674335002899,
-0.028046317398548126,
-0.02226477488875389,
0.08704391121864319,
0.045039258897304535,
0.10495669394731522,
0.06517899036407471,
-0.06165103241801262,
0.00797548983246088,
0.07925331592559814,
-0.03384167701005936,
0.03341482952237129,
0.010629202239215374,
-0.0031566517427563667,
-0.007373101077973843,
0.12512551248073578,
-0.023296605795621872,
0.03352181240916252,
0.049440160393714905,
-0.012367078103125095,
0.05054257810115814,
0.011699858121573925,
0.00659157382324338,
-0.03247714787721634,
-0.033208079636096954,
0.027553731575608253,
-0.023032957687973976,
0.01353207603096962,
0.058660030364990234,
0.016437070444226265,
0.013320394791662693,
-0.041622333228588104,
-0.034786950796842575,
-0.054898396134376526,
0.03608138486742973,
-0.06849241256713867,
-0.033240579068660736,
0.053492363542318344,
-0.0568590983748436,
-0.054840534925460815,
-0.10846737772226334,
-0.005233963951468468,
-0.01367784384638071,
-0.032324936240911484,
-0.009385881945490837,
0.0361793078482151,
0.025019019842147827,
-0.044366586953401566,
-0.021485812962055206,
-0.018265660852193832,
-0.005282936152070761,
0.05151199549436569,
0.0021429082844406366,
0.0306977741420269,
-0.06487756222486496,
0.03363651782274246,
0.10451138764619827,
-0.0073588513769209385
] |
PMID:18551
|
MMPI changes in briefly hospitalized non-narcotic drug users.
|
The Minnesota Multiphasic Personality Inventory (MMPI) was administered to 66 non-narcotic drug abusers as part of an intensive study of polydrug abuse. Patients were classified into four primary drug-of-abuse groups using either stimulants, barbiturates, other sedative-hypnotics, or a combination of these drugs. It was readministered to 42 of these patients after 2 weeks' hospitalization. At admission, the group's MMPI profile was consistent with psychosis. At discharge, most of the MMPI T-scores were sharply reduced, and the groups' profile was consistent with sociopathy. The admission MMPI profiles of the four primary drug-of-abuse groups did not differ. At discharge, the stimulant group's profile remained psychotic, while the profiles of the other three groups were not psychotic. Such results raise the possibility of a toxic psychotic effect of chronic non-narcotic drug abuse. The group of stimulant abusers appeared schizophrenic, while the other groups of non-narcotic drug abusers appeared sociopathic.
|
MMPI changes in briefly hospitalized non-narcotic drug users. The Minnesota Multiphasic Personality Inventory (MMPI) was administered to 66 non-narcotic drug abusers as part of an intensive study of polydrug abuse. Patients were classified into four primary drug-of-abuse groups using either stimulants, barbiturates, other sedative-hypnotics, or a combination of these drugs. It was readministered to 42 of these patients after 2 weeks' hospitalization. At admission, the group's MMPI profile was consistent with psychosis. At discharge, most of the MMPI T-scores were sharply reduced, and the groups' profile was consistent with sociopathy. The admission MMPI profiles of the four primary drug-of-abuse groups did not differ. At discharge, the stimulant group's profile remained psychotic, while the profiles of the other three groups were not psychotic. Such results raise the possibility of a toxic psychotic effect of chronic non-narcotic drug abuse. The group of stimulant abusers appeared schizophrenic, while the other groups of non-narcotic drug abusers appeared sociopathic.
|
[
-0.0036299689672887325,
-0.007572835776954889,
-0.012894639745354652,
-0.010323800146579742,
0.0111853564158082,
0.01590389385819435,
0.039898257702589035,
0.02455757185816765,
-0.042762596160173416,
-0.020554883405566216,
0.05561661720275879,
0.005028028041124344,
0.006114977411925793,
-0.01905209757387638,
-0.031834349036216736,
0.043427933007478714,
0.022563274949789047,
-0.047051507979631424,
0.0014386457623913884,
0.010297116823494434,
-0.04441060125827789,
0.013805018737912178,
0.033120013773441315,
0.021800434216856956,
0.029163124039769173,
0.05356453359127045,
0.016697639599442482,
-0.025900710374116898,
-0.061022426933050156,
-0.14031653106212616,
0.015986710786819458,
0.045678865164518356,
-0.0024000126868486404,
-0.0150120435282588,
-0.0699380487203598,
-0.015340420417487621,
-0.001206895220093429,
0.06447738409042358,
0.02373240701854229,
0.023258714005351067,
0.030011041089892387,
0.06281982362270355,
0.00912119634449482,
0.044117458164691925,
-0.036222610622644424,
-0.040411777794361115,
-0.07732600718736649,
-0.034216880798339844,
0.09766179323196411,
-0.002494712360203266,
-0.042418912053108215,
-0.07952139526605606,
0.0497906357049942,
0.11937166750431061,
-0.0004421021731104702,
-0.043172698467969894,
-0.03290292248129845,
-0.008683916181325912,
-0.03241272270679474,
0.020739881321787834,
-0.006631847936660051,
0.04293235391378403,
-0.11816203594207764,
0.014605982229113579,
0.08114514499902725,
-0.0145659064874053,
-0.058151066303253174,
-0.0664469450712204,
0.03138945996761322,
0.006426763255149126,
-0.05293209105730057,
0.024784881621599197,
-0.019691932946443558,
0.026659881696105003,
0.06519488245248795,
-0.043804362416267395,
-0.006387334316968918,
0.03674088791012764,
0.00844493042677641,
0.01785038784146309,
0.026120096445083618,
-0.019735272973775864,
-0.03519952669739723,
-0.06762633472681046,
0.009258314035832882,
-0.00678971316665411,
0.00953940860927105,
-0.03888788819313049,
-0.010317236185073853,
-0.025220796465873718,
-0.05696683004498482,
-0.006844916380941868,
0.021584857255220413,
-0.047381654381752014,
-0.024867180734872818,
-0.0026372200809419155,
0.009688427671790123,
-0.00798201933503151,
-0.0030567129142582417,
0.37836095690727234,
-0.020746193826198578,
0.032847315073013306,
-0.03978366777300835,
-0.009634695947170258,
-0.0049135927110910416,
0.02079777792096138,
0.06884457916021347,
-0.04536355659365654,
-0.004851127974689007,
0.016561836004257202,
0.05459754541516304,
0.00886476505547762,
0.09709946811199188,
-0.05034365877509117,
-0.021572953090071678,
-0.03174397349357605,
0.024836208671331406,
-0.003577709896489978,
0.06258104741573334,
0.02696760557591915,
-0.026345843449234962,
0.03424239903688431,
0.06479162722826004,
-0.025801796466112137,
0.0327388197183609,
0.022762276232242584,
-0.04696262627840042,
0.08869592100381851,
0.0043062567710876465,
-0.03720995411276817,
-0.008732251822948456,
-0.015601257793605328,
-0.019012412056326866,
0.0206625796854496,
0.0028757613617926836,
0.016800476238131523,
0.009315959177911282,
-0.012710765935480595,
0.08803977072238922,
0.04202556610107422,
-0.03283847123384476,
-0.036455970257520676,
0.02304510585963726,
-0.03349555656313896,
-0.05429811403155327,
0.10645973682403564,
-0.02010408602654934,
-0.04118240997195244,
-0.015918917953968048,
0.04772273078560829,
-0.0006245099357329309,
0.08463070541620255,
-0.09326517581939697,
0.01664423756301403,
-0.037221215665340424,
-0.036819688975811005,
0.036535102874040604,
0.010429974645376205,
-0.026540720835328102,
0.025845030322670937,
-0.007976485416293144,
-0.02051376737654209,
0.02232084795832634,
0.09682576358318329,
0.03671300783753395,
0.01487016025930643,
-0.02541218139231205,
-0.05624881759285927,
-0.05033884942531586,
-0.0800795704126358,
0.04909791797399521,
0.015535436570644379,
-0.029805224388837814,
0.03939903527498245,
-0.021846596151590347,
-0.042430512607097626,
-0.013960741460323334,
0.024487514048814774,
-0.026206672191619873,
0.0032364018261432648,
0.011865009553730488,
0.019031865522265434,
-0.03066996857523918,
0.02357008494436741,
0.001804826781153679,
0.06075038015842438,
-0.01923776976764202,
0.009275703690946102,
0.0630093365907669,
-0.060578059405088425,
0.002697098534554243,
0.018537240102887154,
-0.054651666432619095,
-0.029036065563559532,
0.027904022485017776,
-0.008627849631011486,
-0.047436125576496124,
-0.018640173599123955,
-0.04930972307920456,
0.01854286901652813,
0.023701025173068047,
0.08350582420825958,
-0.01377192884683609,
0.07479579746723175,
-0.0065574124455451965,
0.037643786519765854,
-0.00868268683552742,
-0.022818727418780327,
0.0455029234290123,
-0.01325534749776125,
-0.018009798601269722,
0.002098934957757592,
-0.027513105422258377,
0.018536226823925972,
-0.008386442437767982,
-0.029338648542761803,
0.02524590492248535,
-0.03925875574350357,
0.013893860392272472,
0.06586828827857971,
0.0033876257948577404,
-0.0024204507935792208,
-0.052039675414562225,
-0.2822859585285187,
-0.013051687739789486,
0.0830216258764267,
0.03742515295743942,
-0.03053855709731579,
0.009235340170562267,
-0.015584836713969707,
0.0006217470509000123,
0.009384749457240105,
0.04227844253182411,
0.047162674367427826,
0.05638714134693146,
-0.0642513856291771,
0.02218671143054962,
-0.008185484446585178,
0.00893250759691,
0.07366553694009781,
-0.03719939664006233,
-0.03735757991671562,
-0.057319849729537964,
-0.006601814180612564,
0.018224868923425674,
-0.02799409069120884,
-0.0040595452301204205,
0.05552278459072113,
-0.02444811724126339,
0.09569711238145828,
0.01813468523323536,
-0.06550164520740509,
-0.006715135648846626,
-0.04008467122912407,
0.07318317145109177,
-0.002579524414613843,
-0.12680816650390625,
0.03801781311631203,
-0.046424902975559235,
-0.021934891119599342,
-0.04166647791862488,
-0.00717094074934721,
0.02846621163189411,
-0.08608698099851608,
0.019748670980334282,
0.011881126090884209,
-0.07181225717067719,
-0.08391763269901276,
-0.01899890974164009,
-0.0009123923955485225,
0.027512913569808006,
-0.014203137718141079,
0.025533568114042282,
-0.004544967785477638,
0.0035018364433199167,
0.022324465215206146,
-0.04175073280930519,
-0.020479893311858177,
-0.0417422354221344,
-0.079081229865551,
-0.08052470535039902,
-0.0380459763109684,
-0.0018388287862762809,
0.008377321995794773,
-0.04460618272423744,
0.07407204061746597,
-0.11207301914691925,
0.028116688132286072,
-0.03872174769639969,
-0.05824069678783417,
-0.037200696766376495,
0.07607270777225494,
0.01900712214410305,
0.01679278165102005,
0.10309240221977234,
0.0038561569526791573,
-0.04966709762811661,
0.045949894934892654,
0.019361672922968864,
-0.0739978551864624,
-0.02109932154417038,
-0.06237122789025307,
0.034101638942956924,
-0.04047100991010666,
0.018931565806269646,
0.03248868137598038,
0.026878153905272484,
-0.009761248715221882,
-0.015499270521104336,
-0.03945611044764519,
0.008390008471906185,
0.012793966569006443,
0.03461645543575287,
-0.012771510519087315,
-0.003181845648214221,
0.015770943835377693,
-0.05150141939520836,
0.011734150350093842,
0.04902264475822449,
-0.2856088876724243,
-0.023386240005493164,
0.018843740224838257,
0.005330175161361694,
0.01695333421230316,
0.026737429201602936,
-0.009690097533166409,
-0.0015283882385119796,
-0.02402762696146965,
-0.005208591464906931,
0.05079503357410431,
0.05669316649436951,
0.1133546382188797,
0.003273944603279233,
-0.022215863689780235,
-0.029642712324857712,
0.044583238661289215,
0.005115350242704153,
0.01667804829776287,
0.003248154418542981,
-0.0014484741259366274,
-0.007975352928042412,
0.12888820469379425,
-0.027892841026186943,
-0.001204289379529655,
0.04725712910294533,
0.02734561637043953,
0.01872824877500534,
-0.024514827877283096,
0.025332173332571983,
0.0893576592206955,
0.013170331716537476,
0.13356083631515503,
0.03369899094104767,
0.040595006197690964,
-0.033087920397520065,
-0.07585520297288895,
0.021692268550395966,
0.019647927954792976,
-0.009608528576791286,
0.015448233112692833,
0.010388266295194626,
-0.014494458213448524,
-0.007306102197617292,
0.10409969091415405,
-0.034768808633089066,
-0.01189084630459547,
-0.027913657948374748,
0.02801588922739029,
0.0074038817547261715,
-0.023172253742814064,
0.026625962927937508,
-0.037814151495695114,
0.0737646147608757,
0.004742043558508158,
0.04356377571821213,
-0.045063141733407974,
-0.014419512823224068,
0.0907462015748024,
0.009488211013376713,
-0.0982029139995575,
0.033266324549913406,
-0.014190036803483963,
0.003960197791457176,
-0.008228042162954807
] |
PMID:18552
|
Inhibition of catecholamine release by alpha-adrenergic activation: interaction with Na, K-ATPase.
|
In rat adrenal gland incubated in vitro, phenylephrine and naphazoline inhibited the release of catecholamines (CA) induced in a medium containing K+ (5.6mM). In K+-free medium or in the presence of ouabain (10(-3) M) phenylephrine and naphazoline had no effect on CA release. The alpha-adrenergic blockers, phentolamine and phenoxybenzamine, increased CA release in a K+-medium but had no effect on CA release from adrenal medulla in a K+-free or ouabain (10(-3) M) containing medium. PGE2 inhibited the release of CA both in the presence and in the absence of K+, as well as in the presence of ouabain (10(-3) M). Activation of alpha-adrenergic receptors in the adrenal medulla by phenylephrine and naphazoline resulted in increased Na, K-ATPase activity but no effect on Mg-ATPase. PGE2 acts through a different mechanism.
|
Inhibition of catecholamine release by alpha-adrenergic activation: interaction with Na, K-ATPase. In rat adrenal gland incubated in vitro, phenylephrine and naphazoline inhibited the release of catecholamines (CA) induced in a medium containing K+ (5.6mM). In K+-free medium or in the presence of ouabain (10(-3) M) phenylephrine and naphazoline had no effect on CA release. The alpha-adrenergic blockers, phentolamine and phenoxybenzamine, increased CA release in a K+-medium but had no effect on CA release from adrenal medulla in a K+-free or ouabain (10(-3) M) containing medium. PGE2 inhibited the release of CA both in the presence and in the absence of K+, as well as in the presence of ouabain (10(-3) M). Activation of alpha-adrenergic receptors in the adrenal medulla by phenylephrine and naphazoline resulted in increased Na, K-ATPase activity but no effect on Mg-ATPase. PGE2 acts through a different mechanism.
|
[
-0.05615350976586342,
0.03529919311404228,
-0.01392117515206337,
0.069313645362854,
0.011385347694158554,
-0.05328353866934776,
0.05856490507721901,
0.00013691007916349918,
-0.029337750747799873,
-0.031311001628637314,
0.0005421838723123074,
-0.03474488481879234,
-0.003279582830145955,
0.09671035408973694,
0.009754293598234653,
0.021050801500678062,
0.025845879688858986,
0.014369528740644455,
-0.10562622547149658,
-0.022300010547041893,
-0.031383950263261795,
0.04435929283499718,
0.016619862988591194,
-0.01896330527961254,
-0.007002136670053005,
0.021187864243984222,
-0.007448534481227398,
-0.016127903014421463,
-0.020335758104920387,
-0.15860764682292938,
-0.02375614643096924,
-0.0050178770907223225,
-0.00011481552792247385,
-0.05099281668663025,
-0.010512885637581348,
0.023897768929600716,
-0.07985271513462067,
0.03871042653918266,
-0.07156011462211609,
0.04526825249195099,
0.0296966303139925,
0.0211898572742939,
0.001770516624674201,
0.041578009724617004,
0.018230080604553223,
0.02786635421216488,
0.02207445353269577,
-0.02666754275560379,
0.10873134434223175,
-0.0042532067745924,
0.047203268855810165,
0.07989121228456497,
-0.0032607035245746374,
0.012016279622912407,
0.05292811989784241,
-0.003724584821611643,
0.011638753116130829,
0.06256208568811417,
-0.054364267736673355,
0.040961217135190964,
0.014650126919150352,
0.03992709517478943,
-0.1962055116891861,
0.0661032497882843,
0.01779170148074627,
-0.010573706589639187,
-0.0167599618434906,
-0.0010474256705492735,
0.0007839268073439598,
0.005713109392672777,
-0.02767166495323181,
0.05846095830202103,
0.018370263278484344,
-0.02055729366838932,
0.001207701861858368,
-0.013770265504717827,
0.019636286422610283,
-0.03168835863471031,
0.0033262104261666536,
0.03555450588464737,
0.025610364973545074,
-0.04862094670534134,
0.019655711948871613,
-0.08897297084331512,
-0.000524937582667917,
0.022340761497616768,
0.08721005916595459,
-0.022027624770998955,
-0.017311211675405502,
-0.055569250136613846,
-0.03340916335582733,
-0.024368099868297577,
-0.008549547754228115,
-0.059780143201351166,
-0.004297444596886635,
-0.008662505075335503,
-0.03297588601708412,
-0.014405524358153343,
-0.044506803154945374,
0.31573131680488586,
0.01371684018522501,
0.03346199914813042,
-0.0930660292506218,
0.005534633994102478,
0.02028222568333149,
0.0028425247874110937,
-0.04056575149297714,
-0.04439368098974228,
-0.009645807556807995,
0.05328630283474922,
0.0475313700735569,
0.002282330533489585,
0.037544138729572296,
-0.09114005416631699,
-0.032118458300828934,
-0.045767348259687424,
0.040559787303209305,
0.05476062744855881,
-0.013334677554666996,
-0.004437367897480726,
-0.030072104185819626,
-0.027738260105252266,
0.009350467473268509,
0.02402963675558567,
0.0006796502275392413,
-0.012285329401493073,
-0.03039085492491722,
0.06839760392904282,
0.051699135452508926,
-0.028650304302573204,
0.06964901834726334,
0.008203214034438133,
-0.044554565101861954,
0.021870730444788933,
-0.003139696316793561,
-0.0393698588013649,
0.0652346983551979,
0.008493417873978615,
-0.01597776636481285,
-0.015429472550749779,
-0.06576276570558548,
-0.07124095410108566,
-0.007512335199862719,
-0.12241274118423462,
-0.02377297729253769,
0.08916530758142471,
0.03127776458859444,
0.023460067808628082,
-0.04831324890255928,
-0.0004926487454213202,
-0.016286153346300125,
0.058977168053388596,
-0.027467146515846252,
0.06746673583984375,
-0.053973615169525146,
-0.0036622427869588137,
-0.022895611822605133,
0.007729151286184788,
0.010585474781692028,
0.002328172093257308,
-0.11527685821056366,
-0.010811109095811844,
0.028718188405036926,
0.03300675377249718,
0.0016532507725059986,
0.01979892887175083,
-0.04665376618504524,
-0.03248036280274391,
-0.010067619383335114,
0.006823957432061434,
-0.040257085114717484,
0.06502480804920197,
-0.03106595389544964,
-0.07496435940265656,
-0.046047378331422806,
-0.04507920891046524,
0.03756941482424736,
0.011461293324828148,
0.090992771089077,
-0.028750477358698845,
0.015833880752325058,
-0.028611456975340843,
0.03094499744474888,
-0.0050020827911794186,
0.02523992210626602,
-0.0397239625453949,
-0.06513579934835434,
0.022372199222445488,
-0.000594852608628571,
0.049393441528081894,
-0.024218540638685226,
-0.011881294660270214,
-0.10610043257474899,
0.0011603923048824072,
-0.059304866939783096,
-0.05428303778171539,
-0.0766795426607132,
0.004563753493130207,
0.009181605651974678,
0.013717785477638245,
0.06598031520843506,
0.05231989920139313,
-0.0276165883988142,
0.0366649329662323,
-0.05652663856744766,
0.054862819612026215,
-0.014506090432405472,
0.04184810072183609,
0.026880409568548203,
-0.001915039960294962,
-0.0030398103408515453,
0.021379098296165466,
0.0013743318850174546,
-0.01092706248164177,
-0.06593989580869675,
0.06466387957334518,
-0.02452605962753296,
0.004997185431420803,
0.09579940885305405,
0.010376699268817902,
0.0744735524058342,
-0.00931039359420538,
0.017160363495349884,
-0.29691991209983826,
0.02076619304716587,
0.044453900307416916,
-0.005514479707926512,
0.04645098000764847,
-0.02427886798977852,
0.07193369418382645,
-0.03829735517501831,
-0.011968100443482399,
-0.04010840877890587,
0.003159370506182313,
0.03487125784158707,
-0.013688432052731514,
0.03691335394978523,
0.034242983907461166,
0.040269579738378525,
0.012413992546498775,
-0.043085187673568726,
0.020274605602025986,
0.026526447385549545,
0.048088181763887405,
-0.016419779509305954,
-0.011789665557444096,
0.025850608944892883,
0.06236760690808296,
0.039803069084882736,
0.11135031282901764,
0.021656449884176254,
-0.037232086062431335,
-0.0013161076931282878,
-0.026081472635269165,
0.08402744680643082,
0.01135519053786993,
-0.09864630550146103,
0.02932782471179962,
-0.025257658213377,
0.05738692730665207,
0.03711533546447754,
0.011732044629752636,
0.05048178881406784,
-0.05199180915951729,
0.020421810448169708,
-0.023599814623594284,
-0.05327389016747475,
-0.07011623680591583,
-0.03538811206817627,
0.011226551607251167,
0.06673109531402588,
0.012010550126433372,
-0.032033778727054596,
0.030605558305978775,
0.05076807364821434,
0.03942093625664711,
-0.047083985060453415,
0.03253185376524925,
-0.010337076149880886,
-0.04507550597190857,
0.03685007616877556,
-0.012416928075253963,
-0.02804356813430786,
0.01622733660042286,
0.03682359680533409,
0.011270207352936268,
-0.010308480821549892,
-0.04109802469611168,
0.038785677403211594,
-0.021020283922553062,
0.017138632014393806,
0.038109514862298965,
0.03813154995441437,
0.01500902883708477,
0.1389109492301941,
0.014873186126351357,
0.0367969311773777,
0.06792917847633362,
-0.012770479544997215,
-0.004926906432956457,
-0.0399637408554554,
-0.04476728290319443,
0.029315989464521408,
-0.03198239207267761,
-0.0782778412103653,
0.10099804401397705,
-0.0038006631657481194,
-0.06220464035868645,
0.018593300133943558,
0.046493999660015106,
-0.01309928111732006,
0.0072325123474001884,
0.026165317744016647,
-0.037864167243242264,
0.052713166922330856,
-0.018820712342858315,
-0.017154820263385773,
0.06127586588263512,
0.005984914023429155,
-0.23563404381275177,
0.0022956235334277153,
0.08947841823101044,
-0.04160318151116371,
-0.046512018889188766,
0.068022221326828,
0.04831390455365181,
-0.050931837409734726,
-0.05934673175215721,
-0.02368072234094143,
-0.021120144054293633,
0.030801238492131233,
0.02393590286374092,
0.07095108181238174,
-0.10777368396520615,
-0.0033381725661456585,
0.049332115799188614,
-0.03384722024202347,
0.051053132861852646,
0.003591494634747505,
0.010549346916377544,
-0.052909351885318756,
0.11356374621391296,
-0.023172978311777115,
0.007007556036114693,
-0.012132213450968266,
0.022524625062942505,
-0.0013861802872270346,
-0.013308675028383732,
-0.0527387335896492,
-0.004767123144119978,
0.009731100872159004,
0.018085086718201637,
-0.0684029832482338,
0.021782901138067245,
0.027704311534762383,
-0.03720357269048691,
0.008216273970901966,
-0.0018904434982687235,
-0.026910053566098213,
-0.03309917077422142,
0.038727324455976486,
-0.08480539917945862,
-0.029924849048256874,
0.03162315487861633,
-0.08342702686786652,
-0.09566015005111694,
-0.04105865955352783,
0.04266277700662613,
-0.00419608922675252,
0.028170978650450706,
-0.059924107044935226,
0.03314318135380745,
0.07458445429801941,
0.009266343899071217,
-0.01123197004199028,
-0.021638624370098114,
-0.0028670437168329954,
0.005674874875694513,
0.02564859949052334,
0.00865139439702034,
-0.01341769564896822,
0.018836086615920067,
0.0678233876824379,
0.05698971077799797
] |
PMID:18553
|
Suppression by GABAergic drugs of the locomotor stimulation induced by morphine, amphetamine, and apomorphine: evidence for both pre- and post-synaptic inhibition of catecholamine systems.
|
Locomotor stimulation induced in mice by morphine and amphetamine was antagonized by pretreatment with gamma-butyrolactone (GBL) and amino-oxyacetic acid (AOAA) at doses which had little effect on saline treated animals. The effects of morphine and AOAA on the turnover of brain catecholamines (CA) were determined by measuring both the accumulation of dopa after inhibition of central aromatic L-amino acid decarboxylase and by measuring the depletion of noradrenaline (NA) after inhibition of tyrosine hydroxylase by alpha-methyltyrosine (alpha-MT). Morphine and AOAA were found to have opposite effects on CA turnover, i.e. morphine caused an increase and AOAA, a decrease. AOAA also antagonized the morphine-induced increase in CA turnover. These data might suggest that the well documented ability of GABAergic drugs to inhibit the firing of DA-containing neurons may be of importance in explaining the present findings. However, the locomotor stimulation induced by the directly-acting CA agonists, apomorphine and clonidine after pretreatment with reserpine and alpha-MT was also inhibited by the GABAergic drugs. It is therefore concluded that the suppressant effects of the GABAergic agents on hypermotility and not solely mediated by their effects on presynaptic CA mechanisms, but also by a postsynaptic inhibition at some point beyond the CA neurons.
|
Suppression by GABAergic drugs of the locomotor stimulation induced by morphine, amphetamine, and apomorphine: evidence for both pre- and post-synaptic inhibition of catecholamine systems. Locomotor stimulation induced in mice by morphine and amphetamine was antagonized by pretreatment with gamma-butyrolactone (GBL) and amino-oxyacetic acid (AOAA) at doses which had little effect on saline treated animals. The effects of morphine and AOAA on the turnover of brain catecholamines (CA) were determined by measuring both the accumulation of dopa after inhibition of central aromatic L-amino acid decarboxylase and by measuring the depletion of noradrenaline (NA) after inhibition of tyrosine hydroxylase by alpha-methyltyrosine (alpha-MT). Morphine and AOAA were found to have opposite effects on CA turnover, i.e. morphine caused an increase and AOAA, a decrease. AOAA also antagonized the morphine-induced increase in CA turnover. These data might suggest that the well documented ability of GABAergic drugs to inhibit the firing of DA-containing neurons may be of importance in explaining the present findings. However, the locomotor stimulation induced by the directly-acting CA agonists, apomorphine and clonidine after pretreatment with reserpine and alpha-MT was also inhibited by the GABAergic drugs. It is therefore concluded that the suppressant effects of the GABAergic agents on hypermotility and not solely mediated by their effects on presynaptic CA mechanisms, but also by a postsynaptic inhibition at some point beyond the CA neurons.
|
[
-0.01913868635892868,
0.007612211629748344,
-0.00008540184353478253,
0.044835858047008514,
-0.02218656800687313,
0.02402886375784874,
0.025402462109923363,
0.018385281786322594,
0.005398099310696125,
-0.05561618134379387,
-0.003639589063823223,
0.0012579442700371146,
0.015072583220899105,
0.07921918481588364,
-0.02235305868089199,
0.04276410862803459,
0.0006117012235336006,
-0.02192334085702896,
-0.021224698051810265,
-0.03652161732316017,
0.0034683779813349247,
0.02711104042828083,
0.007470016833394766,
-0.0016271803760901093,
0.02369518205523491,
-0.00811602920293808,
-0.048108458518981934,
-0.017446817830204964,
-0.020451104268431664,
-0.16496945917606354,
-0.013344241306185722,
0.008429666049778461,
0.022190971300005913,
-0.08673839271068573,
-0.03892826661467552,
0.0489322803914547,
-0.04296046495437622,
0.025126958265900612,
-0.050556767731904984,
0.07557147741317749,
0.05174299329519272,
0.03221753612160683,
0.0033469372428953648,
0.008299601264297962,
0.04113375023007393,
0.0021902660373598337,
-0.021251706406474113,
-0.05194493383169174,
0.08911419659852982,
-0.03422056511044502,
0.046727851033210754,
-0.02855658158659935,
0.009886572137475014,
0.032656505703926086,
0.0425850972533226,
-0.004294378682971001,
0.015043724328279495,
0.04983524978160858,
-0.03487792983651161,
0.09102629125118256,
-0.02595674805343151,
0.03213544189929962,
-0.13600270450115204,
0.025713106617331505,
0.06797048449516296,
0.017871348187327385,
-0.04593611881136894,
-0.034351903945207596,
0.07935631275177002,
0.07498510926961899,
-0.05700959265232086,
0.034398145973682404,
-0.007732940372079611,
-0.01562461443245411,
0.027110310271382332,
-0.022848913446068764,
0.0006607191753573716,
0.03903880715370178,
0.044789738953113556,
-0.030921421945095062,
0.04536369815468788,
0.01201505959033966,
0.04946388304233551,
-0.03733639419078827,
-0.02020103856921196,
-0.07674956321716309,
0.046855662018060684,
-0.04344043508172035,
-0.009567400440573692,
0.003011140273883939,
-0.02701611816883087,
-0.02005133032798767,
0.05279187113046646,
-0.041686881333589554,
-0.038319606333971024,
-0.03282039985060692,
-0.018819140270352364,
0.006143652833998203,
-0.04345019534230232,
0.3416874408721924,
0.007535132579505444,
0.035088665783405304,
-0.04591013118624687,
-0.015083840116858482,
0.016070760786533356,
-0.006459102034568787,
0.03463806211948395,
0.019075648859143257,
-0.03184259310364723,
0.08663374185562134,
0.08863722532987595,
0.0014916514046490192,
0.01990213245153427,
0.027710827067494392,
-0.038280077278614044,
-0.05009439215064049,
0.07736367732286453,
0.007657805923372507,
0.04007769003510475,
-0.035193756222724915,
-0.05176401883363724,
-0.021350501105189323,
0.019207848235964775,
0.04003904014825821,
0.029403721913695335,
-0.03130227327346802,
-0.03262513130903244,
0.0769534483551979,
0.03550240769982338,
-0.0233597494661808,
0.0607902966439724,
-0.03777230530977249,
0.015673089772462845,
0.03838488087058067,
-0.01670900173485279,
-0.01536787673830986,
0.06852451711893082,
-0.037971269339323044,
-0.02374998852610588,
0.014731146395206451,
-0.005736207589507103,
-0.011152786202728748,
0.050835758447647095,
-0.04158471152186394,
-0.02981322817504406,
0.06510166823863983,
0.03568496182560921,
-0.013775157742202282,
-0.026791486889123917,
0.014440055936574936,
-0.007890399545431137,
0.053379036486148834,
-0.02721904031932354,
0.09663304686546326,
-0.08159665763378143,
0.06540471315383911,
0.023562170565128326,
0.03937024623155594,
-0.04407036677002907,
-0.013971843756735325,
-0.08248302340507507,
-0.002183526987209916,
-0.01624523475766182,
0.06521137803792953,
0.0019621995743364096,
0.037532590329647064,
-0.045068345963954926,
0.0039009645115584135,
-0.07350517809391022,
0.004413357004523277,
-0.009177069179713726,
0.012779138050973415,
-0.05395539104938507,
-0.05124105140566826,
-0.030916398391127586,
-0.02902565337717533,
0.05557350069284439,
0.059877391904592514,
0.013812114484608173,
-0.05998342111706734,
0.012409262359142303,
-0.028151437640190125,
0.013408245518803596,
-0.004473445937037468,
0.01608559675514698,
0.052671536803245544,
-0.03697453439235687,
-0.040556177496910095,
-0.0121811768040061,
0.013085083104670048,
0.01265790592879057,
-0.02811725251376629,
-0.10420162975788116,
-0.05286635458469391,
0.024708397686481476,
-0.0008931144839152694,
-0.053407326340675354,
0.02184590883553028,
-0.014467064291238785,
-0.02620551735162735,
0.0706205815076828,
0.06188662350177765,
-0.05060054361820221,
0.08840972930192947,
-0.007450002245604992,
0.011583412066102028,
-0.04468318447470665,
0.012387790717184544,
0.022303344681859016,
-0.00933524128049612,
0.0012336736544966698,
-0.015944987535476685,
0.031092332676053047,
-0.0031779382843524218,
-0.05681634694337845,
0.09530942142009735,
-0.0564788356423378,
0.018361279740929604,
0.0370471365749836,
0.002272786805406213,
0.025790300220251083,
-0.040689002722501755,
0.00534233869984746,
-0.2863098680973053,
-0.014619397930800915,
0.0265504140406847,
-0.005977810360491276,
0.05092168599367142,
-0.064405657351017,
-0.021968429908156395,
-0.03777293488383293,
0.0034488572273403406,
-0.01936514861881733,
0.01692178100347519,
0.06875590980052948,
-0.028371471911668777,
0.010506819002330303,
-0.008629129268229008,
0.05567272752523422,
0.009761744178831577,
-0.03727341443300247,
0.043378185480833054,
0.029549019411206245,
0.010025624185800552,
-0.022411594167351723,
-0.039216089993715286,
-0.0202686358243227,
-0.0005404720432125032,
-0.0056654601357877254,
0.1055162101984024,
0.03337661921977997,
-0.005911118816584349,
-0.05734335258603096,
-0.03902771323919296,
0.09262914955615997,
-0.01671692542731762,
-0.10358293354511261,
-0.02193659543991089,
-0.012967037968337536,
0.039325058460235596,
-0.023463109508156776,
0.014434493146836758,
0.017212139442563057,
-0.08368367701768875,
0.03593471646308899,
-0.009375268593430519,
-0.06559472531080246,
-0.053765639662742615,
-0.035599350929260254,
-0.013315150514245033,
0.01220632903277874,
-0.003006762359291315,
-0.02909115143120289,
0.09537509828805923,
0.02603965625166893,
0.020885176956653595,
-0.04516466334462166,
0.02309875749051571,
-0.014749051071703434,
-0.053962476551532745,
-0.03637050464749336,
-0.044576097279787064,
-0.04701827093958855,
-0.013990970328450203,
0.011835874058306217,
0.013262620195746422,
-0.0028690434992313385,
0.007967375218868256,
0.03891191631555557,
0.0018076227279379964,
-0.003113027662038803,
0.07515943050384521,
0.06117707118391991,
0.03630439192056656,
0.12655459344387054,
-0.01894553191959858,
-0.0326179638504982,
0.060199886560440063,
0.0001838786411099136,
-0.0934673547744751,
-0.033943165093660355,
-0.09019007533788681,
0.02019374631345272,
-0.10823148488998413,
-0.008128500543534756,
0.04140631482005119,
0.029856083914637566,
-0.044103048741817474,
-0.028286626562476158,
0.05605755373835564,
-0.023635700345039368,
0.05472712218761444,
0.0016753654927015305,
0.03947839513421059,
0.03270692750811577,
-0.013343323022127151,
-0.06375989317893982,
0.038124796003103256,
0.08996979147195816,
-0.24849575757980347,
-0.012095068581402302,
0.08069942146539688,
-0.0023681973107159138,
0.01466191653162241,
0.030389441177248955,
0.0033028102479875088,
-0.03430707007646561,
-0.06984040141105652,
-0.00698307529091835,
-0.023495065048336983,
0.03931354731321335,
0.05589556321501732,
0.05058155953884125,
-0.011218271218240261,
-0.013974075205624104,
0.08847080171108246,
0.0031669705640524626,
0.03640284016728401,
-0.015743957832455635,
-0.04451665282249451,
0.020764252170920372,
0.1127014085650444,
0.008037541061639786,
-0.01540481485426426,
-0.00889284536242485,
-0.006709636654704809,
0.004381169565021992,
-0.05742110311985016,
-0.051245320588350296,
-0.04073116183280945,
-0.014836695045232773,
0.07703220844268799,
-0.0003056964196730405,
0.07001182436943054,
0.02646218053996563,
-0.0652570053935051,
0.0567137785255909,
0.014307321049273014,
0.024483151733875275,
-0.03166486322879791,
0.0738077238202095,
-0.07090356200933456,
-0.03565391153097153,
0.10851028561592102,
-0.07664230465888977,
-0.07798758149147034,
-0.041833870112895966,
0.0060429745353758335,
0.002946834545582533,
-0.018529996275901794,
-0.04055653139948845,
0.006701919250190258,
0.04565148055553436,
-0.02483421377837658,
-0.016298634931445122,
-0.025909408926963806,
0.025115374475717545,
0.05050567910075188,
-0.0409826897084713,
-0.07431820780038834,
-0.028293365612626076,
-0.03296174854040146,
0.06752511858940125,
0.06552992016077042
] |
PMID:18565
|
Metabolic fate of cyanocobalamin taken up by Spirometra mansonoides spargana.
|
Analysis of tissue from Spirometra mansonoides spargana has shown that cyanocobalamin (vitamin B12) is metabolized to adenosylcobalamin and hydroxocobalamin. No methylcobalamin was detected. When the tissues were examined for enzymes which are known to utilize coenzyme forms of vitamin B12, only methylmalonyl CoA mutase, which requires adenosylcobalamin was found. The enzyme, tetrahydropteroylglutamate methyltransferase, which requires methylcobalamin as a cofactor, was not detected. A sizable portion of the cyanocobalamin taken up was bound to ammonium sulfate-precipitable material, suggesting that the binding substance is a protein. Vitamin B12 taken up by spargana was found to be released in vivo with a biological half-life of about 7 weeks.
|
Metabolic fate of cyanocobalamin taken up by Spirometra mansonoides spargana. Analysis of tissue from Spirometra mansonoides spargana has shown that cyanocobalamin (vitamin B12) is metabolized to adenosylcobalamin and hydroxocobalamin. No methylcobalamin was detected. When the tissues were examined for enzymes which are known to utilize coenzyme forms of vitamin B12, only methylmalonyl CoA mutase, which requires adenosylcobalamin was found. The enzyme, tetrahydropteroylglutamate methyltransferase, which requires methylcobalamin as a cofactor, was not detected. A sizable portion of the cyanocobalamin taken up was bound to ammonium sulfate-precipitable material, suggesting that the binding substance is a protein. Vitamin B12 taken up by spargana was found to be released in vivo with a biological half-life of about 7 weeks.
|
[
-0.06409012526273727,
0.008631984703242779,
-0.08010319620370865,
0.013561757281422615,
0.046365685760974884,
-0.036444712430238724,
0.05174729600548744,
0.024081071838736534,
-0.0533880740404129,
0.019507691264152527,
0.08635225147008896,
-0.08678622543811798,
0.04023589938879013,
-0.01634553074836731,
0.040810417383909225,
-0.01967438869178295,
0.023316942155361176,
0.008227277547121048,
-0.04720785841345787,
0.0009758605738170445,
-0.034580837935209274,
0.0006904494948685169,
0.0222009290009737,
-0.011114177294075489,
0.013799438253045082,
0.02073829434812069,
-0.06040307506918907,
-0.009722411632537842,
-0.09248943626880646,
-0.1912572979927063,
0.019151028245687485,
-0.024634532630443573,
-0.033998042345047,
-0.04199447110295296,
0.008741255849599838,
0.011175268329679966,
0.0023283150512725115,
-0.047045305371284485,
-0.014865054748952389,
0.07968888431787491,
0.025689320638775826,
0.059539780020713806,
0.023024415597319603,
0.01951616257429123,
-0.015943115577101707,
0.03974273055791855,
-0.0340825617313385,
0.016221873462200165,
0.11007097363471985,
-0.015613722614943981,
-0.060610879212617874,
-0.004300712142139673,
-0.0003467676870059222,
0.07127224653959274,
-0.025923684239387512,
0.030970685184001923,
-0.010878404602408409,
0.010743335820734501,
0.04784167930483818,
0.07748596370220184,
-0.015484167262911797,
0.008325864560902119,
-0.1207030639052391,
0.04480867087841034,
0.043609555810689926,
0.047988928854465485,
-0.004863225854933262,
-0.1356084793806076,
0.0498565174639225,
0.016643833369016647,
-0.037322886288166046,
-0.018155254423618317,
0.018252335488796234,
-0.03357711434364319,
-0.0014957762323319912,
-0.011520219035446644,
0.006876706145703793,
-0.029011379927396774,
0.032525770366191864,
0.03487424552440643,
-0.03155055269598961,
0.07420437783002853,
0.029507791623473167,
-0.009417182765901089,
0.05372092127799988,
0.004294001497328281,
0.00017618773563299328,
-0.006269107572734356,
0.009680376388132572,
0.04402365908026695,
-0.003999567590653896,
-0.076406329870224,
-0.010103150270879269,
0.0004455340385902673,
-0.0718063935637474,
0.0034537820611149073,
-0.010411201044917107,
0.04702642560005188,
0.0660119503736496,
0.34010088443756104,
0.018353212624788284,
-0.009409947320818901,
-0.07209022343158722,
-0.007836534641683102,
0.0024709836579859257,
-0.027030356228351593,
-0.0005610567168332636,
0.013451597653329372,
0.020348912104964256,
0.017596496269106865,
0.06573797762393951,
0.04085730388760567,
0.018923399969935417,
-0.0231906957924366,
-0.04370216652750969,
0.005326283164322376,
0.018586326390504837,
-0.0379924401640892,
-0.06111526861786842,
0.04939262941479683,
-0.018238961696624756,
-0.001695848535746336,
0.09268772602081299,
-0.045522525906562805,
-0.0012823716970160604,
0.05573492869734764,
0.05050671473145485,
0.06319987028837204,
0.06308230757713318,
0.040492329746484756,
0.07191876322031021,
0.024992823600769043,
-0.024060972034931183,
0.014444668777287006,
-0.06454482674598694,
0.0073020318523049355,
0.09223493188619614,
-0.026518642902374268,
0.0008620938751846552,
0.0023793508298695087,
-0.02938094362616539,
-0.04458500072360039,
-0.012406069785356522,
-0.09041094779968262,
-0.039109185338020325,
0.09884698688983917,
0.00324126030318439,
-0.0015836501261219382,
0.02344151958823204,
-0.007890500128269196,
-0.014524045400321484,
0.036712076514959335,
0.003365943906828761,
0.02948674000799656,
-0.05431636422872543,
0.03652985766530037,
-0.04970499500632286,
-0.019418446347117424,
0.018813278526067734,
-0.03105480596423149,
-0.06901492178440094,
-0.036168936640024185,
-0.01184045523405075,
0.05489130690693855,
0.006757248658686876,
-0.060773443430662155,
0.005989724304527044,
-0.007885603234171867,
-0.030896643176674843,
0.040858715772628784,
0.026897341012954712,
-0.029737252742052078,
0.003359283087775111,
-0.020411087200045586,
-0.002329806564375758,
0.03513380140066147,
0.013982130214571953,
0.049501389265060425,
0.023380201309919357,
-0.0023450700100511312,
0.024984098970890045,
0.04816754534840584,
0.022717861458659172,
0.06228652596473694,
0.0876443088054657,
0.01774834655225277,
-0.023391464725136757,
0.007177672348916531,
-0.030305400490760803,
0.008866550400853157,
-0.053403791040182114,
-0.0010437601013109088,
-0.07694272696971893,
-0.044027991592884064,
-0.038625895977020264,
-0.03281958028674126,
-0.0700470507144928,
0.0046525378711521626,
-0.013671248219907284,
-0.056940969079732895,
0.07349853962659836,
0.009792517870664597,
0.055403932929039,
0.028195921331644058,
0.0038497785571962595,
-0.003143230453133583,
0.01787683181464672,
0.01766003668308258,
-0.0014856756897643209,
0.017330484464764595,
0.02151891589164734,
0.011162524111568928,
-0.04828459769487381,
0.058386512100696564,
-0.030878668650984764,
0.05480574071407318,
0.018715638667345047,
0.015141704119741917,
0.03451066091656685,
0.05207260325551033,
0.021194618195295334,
-0.07819776982069016,
-0.07925436645746231,
-0.3321721851825714,
-0.023007556796073914,
-0.01816296949982643,
-0.0014875555643811822,
-0.009408959187567234,
-0.05936989188194275,
0.0084898816421628,
0.007797962985932827,
0.005138244479894638,
-0.004489201121032238,
0.06015925854444504,
0.05848447605967522,
-0.03714674711227417,
-0.07909850031137466,
-0.05635198950767517,
0.02603396214544773,
0.05553059279918671,
-0.03439965844154358,
0.020834246650338173,
0.02956329844892025,
-0.04018170386552811,
-0.08325842022895813,
0.02916089817881584,
0.003982935566455126,
0.07622891664505005,
-0.024312641471624374,
0.1216587945818901,
0.013707167468965054,
-0.0026543266139924526,
-0.01866650953888893,
0.022828372195363045,
-0.010950004681944847,
0.018623534590005875,
-0.06708571314811707,
-0.022817347198724747,
-0.08945715427398682,
0.00494898296892643,
-0.043772682547569275,
0.0067826141603291035,
-0.03108822926878929,
-0.02803860418498516,
0.02581387758255005,
-0.002101657912135124,
-0.07270553708076477,
-0.05383346602320671,
0.032198984175920486,
-0.024558175355196,
0.01624353416264057,
0.018708348274230957,
-0.0005747753311879933,
0.029973415657877922,
0.09805238246917725,
0.012851144187152386,
0.017684917896986008,
0.027076480910182,
-0.0005173159879632294,
-0.024371378123760223,
-0.029682444408535957,
-0.04656994715332985,
0.005094198510050774,
-0.06537918001413345,
0.01866125874221325,
0.01894730143249035,
0.026693077757954597,
0.020693814381957054,
0.04140915721654892,
0.03420501574873924,
-0.07135028392076492,
0.07379361242055893,
0.004511077422648668,
0.031445764005184174,
0.10351581126451492,
-0.04426297917962074,
-0.022729551419615746,
0.030449511483311653,
0.06858761608600616,
0.014624285511672497,
-0.07074598222970963,
-0.060204558074474335,
-0.017009194940328598,
0.03962937369942665,
0.008136696182191372,
-0.008566810749471188,
0.04901673644781113,
-0.02238333411514759,
0.014132562093436718,
-0.00822184793651104,
0.009549597278237343,
0.0023604121524840593,
0.03231637552380562,
0.014919467270374298,
-0.015242236666381359,
-0.013759469613432884,
-0.023460563272237778,
0.027901330962777138,
0.06839397549629211,
-0.23914000391960144,
0.05458296090364456,
-0.005160143598914146,
-0.03546750545501709,
0.02239164710044861,
-0.0029506206046789885,
0.042490873485803604,
-0.03335598483681679,
-0.05154630169272423,
0.06650391221046448,
0.04026108235120773,
-0.009945936501026154,
0.10096493363380432,
0.07189735025167465,
0.011213511228561401,
-0.012495176866650581,
0.03186536207795143,
-0.015268777497112751,
-0.025122148916125298,
-0.04002702236175537,
-0.03052782267332077,
-0.05146106332540512,
0.12880384922027588,
0.03791821002960205,
-0.016379719600081444,
-0.013083168305456638,
-0.02046491764485836,
0.03916509449481964,
0.001410561497323215,
0.046677686274051666,
0.011003123596310616,
0.024566520005464554,
0.0498742051422596,
-0.015237678773701191,
-0.0322314128279686,
0.014835950918495655,
-0.047067560255527496,
-0.0369114875793457,
0.0074953483417630196,
0.0065103499218821526,
-0.0464935265481472,
-0.022644827142357826,
-0.0689319372177124,
0.014953752048313618,
0.059508658945560455,
-0.058865562081336975,
-0.05638134479522705,
-0.05001304671168327,
0.04006344825029373,
-0.006197068840265274,
-0.03654183819890022,
-0.011132271960377693,
0.0006871125660836697,
0.0007768473005853593,
-0.006833185907453299,
0.040771253407001495,
0.017935415729880333,
-0.02253817208111286,
-0.03676620498299599,
-0.02034984529018402,
-0.014215066097676754,
-0.07480249553918839,
-0.08864293992519379,
0.10118866711854935,
-0.0347745381295681
] |
PMID:18568
|
The pharmacology of benoxaprofen (2-[4-chlorophenyl]-alpha-methyl-5-benzoxazole acetic acid), LRCL 3794, a new compound with antiinflammatory activity apparently unrelated to inhibition of prostaglandin synthesis.
|
Benoxaprofen is a potent and long-acting anti-inflammatory and antipyretic compound. Its anti-inflammatory activity has been demonstrated in carrageenan-induced oedema, in cellulose pellet granuloma and in both developing and established adjuvant arthritis tests in rats. Its antipyretic activity is greater than either aspirin or paracetamol in tests inducing pyrexia with yeast of 'E' pyrogen in rats and rabbits. Benoxaprofen has analgesic activity in tests where pain is accompanied by inflammation but not in other experimental models of pain. The weak prostaglandin synthetase inhibiting properties of this compound differentiate it from other acid anti-inflammatory compounds. The low ulcerogenic potential of benoxaprofen seen in animal models may be related to its relative inability to inhibit PG synthetase.
|
The pharmacology of benoxaprofen (2-[4-chlorophenyl]-alpha-methyl-5-benzoxazole acetic acid), LRCL 3794, a new compound with antiinflammatory activity apparently unrelated to inhibition of prostaglandin synthesis. Benoxaprofen is a potent and long-acting anti-inflammatory and antipyretic compound. Its anti-inflammatory activity has been demonstrated in carrageenan-induced oedema, in cellulose pellet granuloma and in both developing and established adjuvant arthritis tests in rats. Its antipyretic activity is greater than either aspirin or paracetamol in tests inducing pyrexia with yeast of 'E' pyrogen in rats and rabbits. Benoxaprofen has analgesic activity in tests where pain is accompanied by inflammation but not in other experimental models of pain. The weak prostaglandin synthetase inhibiting properties of this compound differentiate it from other acid anti-inflammatory compounds. The low ulcerogenic potential of benoxaprofen seen in animal models may be related to its relative inability to inhibit PG synthetase.
|
[
-0.04328656196594238,
-0.0423203706741333,
-0.027331504970788956,
0.015067805536091328,
0.022115984931588173,
0.005652950145304203,
0.04798136278986931,
0.0757196694612503,
-0.11878340691328049,
-0.03235938772559166,
0.04213816300034523,
-0.018194006755948067,
0.02933860570192337,
-0.010373728349804878,
-0.0574079193174839,
-0.012917287647724152,
0.012677263468503952,
-0.023716017603874207,
-0.021370824426412582,
0.000505089177750051,
-0.011196592822670937,
0.026898205280303955,
0.07137510180473328,
0.03325175121426582,
0.05025003105401993,
0.0312933512032032,
0.0608438104391098,
-0.009135877713561058,
-0.01717352494597435,
-0.1470002681016922,
0.008786243386566639,
-0.003782563144341111,
-0.010566812939941883,
-0.009464661590754986,
0.0179030392318964,
-0.0028545812238007784,
-0.06391042470932007,
0.042271241545677185,
-0.021527018398046494,
-0.001560209901072085,
0.08242706209421158,
0.0016005486249923706,
-0.02308964915573597,
0.08521751314401627,
0.05822871997952461,
-0.006620583590120077,
-0.05457640811800957,
0.040581416338682175,
0.07385395467281342,
0.03531021997332573,
0.08999688923358917,
-0.040590643882751465,
-0.03196693956851959,
0.008203929290175438,
0.06545205414295197,
-0.015128976665437222,
-0.027821971103549004,
0.04648088663816452,
-0.04530582204461098,
0.009979425929486752,
0.03672424703836441,
0.03886827453970909,
-0.10323233902454376,
0.051195431500673294,
0.013945791870355606,
-0.03897792100906372,
0.0010213652858510613,
-0.06437653303146362,
0.06363184750080109,
0.06150016188621521,
-0.04087182879447937,
0.061771001666784286,
-0.0021354802884161472,
0.014351091347634792,
0.01878008060157299,
0.034996148198843,
0.026345571503043175,
-0.028011716902256012,
0.013425657525658607,
0.03595506772398949,
0.036589525640010834,
0.017815126106142998,
0.0020702567417174578,
-0.0353621244430542,
0.017239004373550415,
-0.028691144660115242,
0.029433270916342735,
-0.046167027205228806,
0.04557645693421364,
-0.02926572598516941,
0.009665533900260925,
-0.01945905014872551,
-0.05464709922671318,
-0.06201295182108879,
-0.012052632868289948,
0.03665942698717117,
-0.036449749022722244,
0.022637756541371346,
-0.07854484021663666,
0.3667267858982086,
-0.028338981792330742,
0.00525246886536479,
-0.07850801199674606,
-0.011346480809152126,
0.02137422189116478,
0.006667909678071737,
0.002812074264511466,
-0.01089262031018734,
-0.06167076900601387,
0.04774151369929314,
0.06591631472110748,
0.01717386208474636,
-0.017035027965903282,
0.03645124286413193,
-0.02554135024547577,
-0.0584896095097065,
0.05463189259171486,
0.031163088977336884,
0.01073617022484541,
0.09545275568962097,
0.009924205020070076,
-0.018861548975110054,
0.039531365036964417,
-0.0052606347016990185,
0.030944619327783585,
0.03114355355501175,
0.022546570748090744,
0.0967705249786377,
0.0371466763317585,
0.03378674387931824,
0.01885577104985714,
0.007247215136885643,
-0.00007540701335528865,
-0.002755158580839634,
-0.02786581590771675,
0.006810187827795744,
0.01075765024870634,
0.05530001223087311,
0.0259572621434927,
0.005741723347455263,
-0.03987858071923256,
-0.0806673988699913,
0.00030463270377367735,
-0.03122936561703682,
-0.09459741413593292,
0.1405530720949173,
0.03995500132441521,
-0.030057638883590698,
-0.04623786732554436,
0.0332634262740612,
0.002488858997821808,
0.026665516197681427,
0.008838463574647903,
0.06611471623182297,
-0.009350097738206387,
-0.015894778072834015,
0.046139005571603775,
-0.020049283280968666,
-0.026906751096248627,
0.039088375866413116,
-0.10279770195484161,
0.019372060894966125,
-0.0037388186901807785,
0.09348093718290329,
-0.06192989647388458,
-0.01827475242316723,
-0.017523828893899918,
0.017480066046118736,
-0.033757686614990234,
0.07517652958631516,
-0.04528508335351944,
0.016470985487103462,
-0.044578030705451965,
-0.03908919170498848,
-0.06305346637964249,
0.06074269115924835,
0.00016956089530140162,
-0.05346156284213066,
0.040473148226737976,
-0.023509914055466652,
0.046964578330516815,
-0.012141665443778038,
-0.019954334944486618,
0.0028388823848217726,
-0.0521470382809639,
0.01853114925324917,
-0.0690448135137558,
-0.008101672865450382,
0.05063649266958237,
-0.03514263406395912,
-0.008542362600564957,
-0.03622778132557869,
-0.026523727923631668,
0.037104588001966476,
-0.09343311935663223,
-0.0773736834526062,
-0.08472352474927902,
-0.014126078225672245,
-0.00613411283120513,
-0.047610536217689514,
0.10456424951553345,
0.050992585718631744,
-0.019875263795256615,
0.022718822583556175,
0.07399297505617142,
0.02130819298326969,
0.0031225557904690504,
0.04982828348875046,
0.007961872965097427,
-0.058267414569854736,
0.02794652432203293,
0.10217785835266113,
-0.0072927530854940414,
-0.010976400226354599,
-0.05460723489522934,
0.07742179930210114,
-0.03396708518266678,
-0.03136756271123886,
0.019439324736595154,
0.02882971428334713,
-0.026783552020788193,
-0.02590426243841648,
0.006997199729084969,
-0.29361459612846375,
0.03318970277905464,
-0.04246542602777481,
-0.03660368546843529,
0.01431142259389162,
0.023691361770033836,
0.03869248926639557,
-0.059613313525915146,
-0.014691521413624287,
0.0166060421615839,
0.07774468511343002,
0.05695842579007149,
-0.026168853044509888,
-0.020358769223093987,
0.006784799974411726,
0.043406471610069275,
0.006913615390658379,
-0.02490215376019478,
0.03788167983293533,
0.005574843380600214,
0.015944015234708786,
-0.06807542592287064,
-0.04759785160422325,
0.02538418024778366,
0.004938501864671707,
-0.01586664468050003,
0.09033767879009247,
0.011764119379222393,
-0.0065408009104430676,
-0.019546519964933395,
0.016509395092725754,
0.03837965056300163,
-0.017996149137616158,
-0.10431963950395584,
-0.03542159125208855,
0.0007211324409581721,
0.05736704170703888,
-0.04183684289455414,
-0.022904234007000923,
-0.006888024974614382,
-0.011759979650378227,
-0.001476742560043931,
-0.006042161025106907,
-0.0917438194155693,
-0.0208576712757349,
-0.006460798904299736,
0.0016930850688368082,
0.0008997134864330292,
-0.032917335629463196,
0.00599317392334342,
-0.03740312531590462,
0.027442004531621933,
-0.0021742316894233227,
0.03933383896946907,
-0.020613279193639755,
0.0001476441539125517,
-0.030587056651711464,
0.012922964990139008,
-0.015604102984070778,
-0.08001796156167984,
-0.03573814779520035,
-0.0037506280932575464,
0.0654163658618927,
0.03552059084177017,
-0.0057614571414887905,
-0.0251659844070673,
0.01629403419792652,
-0.08484194427728653,
0.022170620039105415,
0.02809339575469494,
0.021230868995189667,
0.1568658947944641,
-0.06501112878322601,
-0.016900600865483284,
0.028413904830813408,
0.05046231299638748,
0.047073327004909515,
-0.040727972984313965,
-0.054453760385513306,
0.017102127894759178,
-0.024349916726350784,
-0.028594940900802612,
-0.00012069619697285816,
-0.06307829916477203,
-0.03272601589560509,
-0.04397705942392349,
-0.02970282733440399,
-0.011149824596941471,
0.06264608353376389,
0.003555268282070756,
0.044845160096883774,
-0.028030354529619217,
0.020638996735215187,
-0.049876682460308075,
0.025458285585045815,
-0.06361804157495499,
-0.24814575910568237,
-0.009318537078797817,
0.05589501932263374,
0.01061516534537077,
-0.012416249141097069,
-0.011984745040535927,
0.009004900231957436,
-0.025899386033415794,
-0.025622533634305,
-0.014167065732181072,
-0.0513199083507061,
0.06067652627825737,
0.03621188923716545,
0.045604437589645386,
-0.048573922365903854,
-0.04186073690652847,
0.05681043863296509,
-0.008920600637793541,
0.005662646144628525,
0.02939078025519848,
0.005469894036650658,
-0.05860716104507446,
0.09933041781187057,
0.026917511597275734,
0.0065340097062289715,
0.08102288842201233,
0.014787242747843266,
-0.04506981000304222,
-0.02645624801516533,
-0.023978019133210182,
0.010799296200275421,
-0.03816915303468704,
0.05105612426996231,
-0.051590774208307266,
0.006506055593490601,
0.06993236392736435,
-0.019975561648607254,
0.05285942181944847,
-0.012003577314317226,
0.02421383187174797,
0.003424650989472866,
-0.05099564790725708,
-0.05432479456067085,
0.00698841642588377,
0.047203123569488525,
-0.05581352487206459,
-0.030751856043934822,
-0.04578786715865135,
-0.004070430528372526,
0.024876615032553673,
-0.0007971037412062287,
-0.02558148466050625,
0.03448141738772392,
0.028035100549459457,
0.03882982209324837,
-0.020438630133867264,
0.02218562923371792,
-0.03892993927001953,
0.02849838137626648,
-0.030908426269888878,
0.01708425022661686,
-0.06866232305765152,
0.004137001000344753,
0.08581210672855377,
0.057925995439291
] |
PMID:18571
|
The in vitro metabolism of 2-nitroso-1-phenylpropane dimer by fortified 9000g supernatants from rabbit liver.
|
2-Nitroso-1-phenylpropane dimer, a postulated metabolic product of amphetamine, was metabolized by fortified 9000 g supernatants from rabbit liver, mainly by oxidation on the carbon beta to the nitrogen to form a "beta-hydroxylated nitroso dimer'. The corresponding nitro compound, ketone and alcohol were also products, the latter two but not the former being cofactor dependent. The nitroso compound was not metabolically reduced under the conditions used.
|
The in vitro metabolism of 2-nitroso-1-phenylpropane dimer by fortified 9000g supernatants from rabbit liver. 2-Nitroso-1-phenylpropane dimer, a postulated metabolic product of amphetamine, was metabolized by fortified 9000 g supernatants from rabbit liver, mainly by oxidation on the carbon beta to the nitrogen to form a "beta-hydroxylated nitroso dimer'. The corresponding nitro compound, ketone and alcohol were also products, the latter two but not the former being cofactor dependent. The nitroso compound was not metabolically reduced under the conditions used.
|
[
-0.07036230713129044,
0.03573209047317505,
-0.019953759387135506,
-0.023233022540807724,
0.037167392671108246,
0.022142283618450165,
0.0304134301841259,
0.0059058754704892635,
-0.07446740567684174,
-0.016518916934728622,
0.010068570263683796,
-0.030907215550541878,
0.03368398919701576,
-0.00816828478127718,
-0.03203533962368965,
-0.013015374541282654,
-0.010971225798130035,
-0.055593088269233704,
-0.03922785073518753,
-0.006588127464056015,
0.049396973103284836,
-0.029644671827554703,
0.048499759286642075,
0.014996787533164024,
0.01654087007045746,
0.018735475838184357,
-0.05096530541777611,
0.029196858406066895,
-0.008316037245094776,
-0.19556446373462677,
0.061718981713056564,
0.03144724667072296,
-0.02505367435514927,
-0.030641397461295128,
-0.037337809801101685,
0.025949375703930855,
0.03534872829914093,
0.013565203174948692,
-0.021983817219734192,
0.015169014222919941,
0.049174848943948746,
0.0013808200601488352,
-0.004998500924557447,
0.01999233476817608,
0.09656926989555359,
0.039643917232751846,
-0.0605224072933197,
0.013935128226876259,
0.1190333366394043,
-0.025840632617473602,
-0.00038571201730519533,
-0.02797883003950119,
0.053868893533945084,
0.0280303955078125,
0.056985411792993546,
-0.03686125949025154,
0.03624559938907623,
-0.008417436853051186,
0.021526286378502846,
0.04835497587919235,
-0.012491719797253609,
0.04411449283361435,
-0.17750512063503265,
0.040751710534095764,
0.015119469724595547,
-0.028163976967334747,
-0.031646959483623505,
-0.019881224259734154,
0.02167704328894615,
0.0591062493622303,
-0.005524547770619392,
-0.01134664285928011,
0.00021335462224669755,
-0.04367375001311302,
-0.024859599769115448,
0.034502215683460236,
-0.0024353573098778725,
0.006050923839211464,
0.015057096257805824,
0.008243344724178314,
0.03245232254266739,
-0.0047953249886631966,
-0.0005013032932765782,
-0.007918357849121094,
0.020378123968839645,
-0.004106591921299696,
-0.021155882626771927,
-0.020292628556489944,
0.025565454736351967,
0.00033523261663503945,
-0.04390788823366165,
-0.019376784563064575,
0.012038503773510456,
-0.05132555216550827,
-0.03630327805876732,
0.031749576330184937,
-0.015162338502705097,
0.005150171462446451,
0.0182926245033741,
0.34931671619415283,
-0.01756809838116169,
-0.007461605127900839,
-0.028585048392415047,
-0.019201574847102165,
-0.032993122935295105,
-0.054094575345516205,
0.023782959207892418,
0.02408222295343876,
0.03751566261053085,
0.01777030900120735,
0.06515133380889893,
0.10395243018865585,
0.018823519349098206,
-0.08471518754959106,
-0.017099184915423393,
-0.03442931920289993,
0.026470452547073364,
-0.0026399455964565277,
-0.006210053339600563,
0.029981203377246857,
0.025872720405459404,
0.03627703711390495,
0.0706673115491867,
0.0007045845268294215,
0.0024867751635611057,
0.031706519424915314,
-0.014204399660229683,
0.06253646314144135,
0.02067403495311737,
0.003420163644477725,
-0.02463006041944027,
0.03089894913136959,
0.05127493664622307,
0.02085103653371334,
-0.07675044238567352,
-0.03522086516022682,
0.08303984254598618,
-0.021511057391762733,
0.024179179221391678,
-0.008864052593708038,
-0.044298429042100906,
-0.017434706911444664,
-0.04831143841147423,
-0.05832280218601227,
-0.05925620347261429,
0.05870871990919113,
0.02149038203060627,
-0.01830533891916275,
-0.017484493553638458,
0.04311564564704895,
0.012351671233773232,
0.030890651047229767,
-0.020201537758111954,
0.008665361441671848,
-0.04415503889322281,
0.03367277979850769,
0.020469406619668007,
-0.009193475358188152,
0.0023767035454511642,
0.012277123518288136,
-0.07956387847661972,
-0.032613739371299744,
0.06497205048799515,
0.07285837084054947,
-0.025221804156899452,
-0.06125345826148987,
-0.03936762735247612,
0.009356898255646229,
0.020037325099110603,
0.003187927883118391,
0.014360633678734303,
-0.038595765829086304,
-0.008701756596565247,
0.022401584312319756,
-0.02184630185365677,
0.03228583186864853,
0.028173096477985382,
0.06724205613136292,
0.003168014343827963,
-0.014318962581455708,
0.05000605061650276,
-0.03935978189110756,
-0.016751978546380997,
0.026946982368826866,
0.01861974038183689,
-0.0033062270376831293,
-0.07837658375501633,
-0.046873558312654495,
-0.007307661697268486,
0.044576436281204224,
-0.035832807421684265,
0.013414179906249046,
-0.09606265276670456,
0.04416310414671898,
-0.01156831905245781,
-0.006695454008877277,
-0.0736844390630722,
0.042913973331451416,
-0.036991942673921585,
-0.04699501022696495,
0.10220527648925781,
-0.010942255146801472,
0.0213665422052145,
0.06065620854496956,
0.023422833532094955,
0.01589852198958397,
0.010215041227638721,
-0.006790918298065662,
0.008635188452899456,
0.0014117429964244366,
-0.0352974608540535,
-0.049940139055252075,
-0.007342685014009476,
0.023431329056620598,
-0.05368642881512642,
0.021174293011426926,
0.0038334291893988848,
0.009283557534217834,
0.07122422754764557,
-0.0012407528702169657,
0.028721526265144348,
-0.08081342279911041,
-0.07806351780891418,
-0.27647411823272705,
0.007340530399233103,
0.002277867402881384,
-0.002162122866138816,
0.021114295348525047,
-0.02039232663810253,
0.01167752593755722,
-0.04258411005139351,
0.01470813900232315,
-0.013622310012578964,
0.013237932696938515,
0.04485958814620972,
0.005793645046651363,
0.0339927151799202,
-0.025217730551958084,
0.03786831721663475,
0.0017191878287121654,
-0.03068724274635315,
0.04969397559762001,
-0.023375114426016808,
0.03323804959654808,
0.005088535603135824,
0.02167884260416031,
0.020152390003204346,
0.0796821266412735,
-0.01157470978796482,
0.15705139935016632,
0.004562165588140488,
-0.002204570220783353,
-0.0007019430631771684,
0.006832194048911333,
0.027874866500496864,
-0.05680492892861366,
-0.10242599993944168,
0.0008189635118469596,
-0.12435579299926758,
0.005983429029583931,
-0.033316172659397125,
0.01482474897056818,
0.00009260407387046143,
0.002046828391030431,
-0.011075432412326336,
0.002827651333063841,
-0.11823974549770355,
-0.06899259239435196,
-0.002662335056811571,
-0.08285275101661682,
-0.03538059443235397,
-0.018738383427262306,
0.01268322579562664,
0.07421291619539261,
0.058751050382852554,
0.025707311928272247,
-0.054749466478824615,
-0.01764102093875408,
-0.018994923681020737,
-0.059906672686338425,
0.014984113164246082,
-0.018333876505494118,
-0.030910653993487358,
-0.017650991678237915,
-0.017726201564073563,
0.02790326066315174,
-0.02588772587478161,
0.017900483682751656,
0.019182633608579636,
0.05889623612165451,
0.013529835268855095,
0.02158547192811966,
0.055608656257390976,
0.04188641160726547,
0.09913592785596848,
-0.06892033666372299,
0.05181892216205597,
0.04329868033528328,
0.013505516573786736,
0.002110757166519761,
-0.033279478549957275,
-0.08808519691228867,
0.04471346735954285,
-0.012726940214633942,
0.0026033814065158367,
0.04588121920824051,
0.055937789380550385,
-0.0061771804466843605,
-0.0056991977617144585,
0.007146241143345833,
0.014510764740407467,
0.056977905333042145,
0.04789746180176735,
0.026034411042928696,
0.048401784151792526,
-0.04406804218888283,
-0.05969501659274101,
0.053170692175626755,
0.062238238751888275,
-0.2864168882369995,
-0.041398100554943085,
0.0030581436585634947,
0.031064629554748535,
0.010104537941515446,
-0.011664200574159622,
0.022536857053637505,
-0.016380978748202324,
-0.01656767725944519,
0.028563275933265686,
0.016953302547335625,
-0.004348349291831255,
0.07586345076560974,
0.05363847315311432,
0.017497852444648743,
-0.014282036572694778,
0.04290197417140007,
-0.022727319970726967,
0.002514517866075039,
-0.06409720331430435,
0.015593568794429302,
-0.025303957983851433,
0.13918133080005646,
-0.002897881669923663,
-0.02603456750512123,
0.025466563180088997,
-0.04502307251095772,
0.05836818367242813,
-0.006620101165026426,
0.019487738609313965,
-0.057203758507966995,
-0.018185241147875786,
0.13602235913276672,
-0.08070896565914154,
0.013592053204774857,
0.03321615606546402,
-0.08261630684137344,
-0.015475690364837646,
-0.039763353765010834,
-0.015221609733998775,
-0.06589266657829285,
-0.016709337010979652,
-0.0376226045191288,
0.00444915983825922,
0.08512605726718903,
-0.06558915972709656,
-0.0469086579978466,
-0.07425900548696518,
-0.011440054513514042,
-0.024426931515336037,
-0.06718485802412033,
0.01655026525259018,
0.0285435002297163,
0.06379993259906769,
0.03187320753931999,
-0.01304229162633419,
0.006070742383599281,
-0.012822523713111877,
-0.004277484025806189,
-0.029893284663558006,
-0.06348644196987152,
0.043072596192359924,
-0.013834996148943901,
0.08611191809177399,
0.0044646901078522205
] |
PMID:18572
|
Application of orthogonal functions to the spectrophotometric determination of phenytoin and phenobarbitone in pharmaceutical preparations.
|
Orthogonal polynomial functions have been used in the simultaneous determination of mixtures of phenytoin and phenobarbitone in pharmaceutical preparations. The mean recovery (9 determinations) and coefficeint of variation were 100-0 +/- 1-4% for phenobarbitone and 100-0 +/-1-3% for phenytoin. Neither the delta A method of Pannerdan & Fontein nor a delta A modification of Vierordt's method of two-component analysis gave results of the same accuracy and precision as the proposed method.
|
Application of orthogonal functions to the spectrophotometric determination of phenytoin and phenobarbitone in pharmaceutical preparations. Orthogonal polynomial functions have been used in the simultaneous determination of mixtures of phenytoin and phenobarbitone in pharmaceutical preparations. The mean recovery (9 determinations) and coefficeint of variation were 100-0 +/- 1-4% for phenobarbitone and 100-0 +/-1-3% for phenytoin. Neither the delta A method of Pannerdan & Fontein nor a delta A modification of Vierordt's method of two-component analysis gave results of the same accuracy and precision as the proposed method.
|
[
-0.10058069229125977,
0.02176845073699951,
0.01528986357152462,
-0.04361289367079735,
0.04865728318691254,
-0.019326334819197655,
0.07656793296337128,
0.051803987473249435,
-0.03173085302114487,
0.021872475743293762,
-0.0364290252327919,
-0.06233510747551918,
0.04126115143299103,
0.012899060733616352,
0.008633522316813469,
-0.016413481906056404,
0.05293554812669754,
0.0036777309142053127,
-0.036901090294122696,
0.0066489968448877335,
0.01930559240281582,
-0.0039016976952552795,
-0.007714072708040476,
-0.025933271273970604,
0.07299657166004181,
0.016889899969100952,
-0.017227524891495705,
0.009299136698246002,
-0.003386355936527252,
-0.19852252304553986,
0.04695318639278412,
0.09486754983663559,
-0.005060284864157438,
-0.10942105203866959,
0.0015476143453270197,
0.03208503499627113,
-0.0089948158711195,
0.03204173967242241,
0.04908766597509384,
0.014556404203176498,
0.038335978984832764,
0.0012720308732241392,
0.0038803487550467253,
0.014716381207108498,
0.02653048187494278,
-0.012105891481041908,
-0.07049790024757385,
0.04554946348071098,
0.037288304418325424,
-0.006265347357839346,
0.024150120094418526,
0.06019142270088196,
0.06998226046562195,
0.012217607349157333,
0.03763137012720108,
-0.013993955217301846,
-0.035461537539958954,
0.008265094831585884,
-0.033017534762620926,
0.07154260575771332,
0.005997174885123968,
0.018358547240495682,
-0.16082848608493805,
0.052564047276973724,
-0.012114597484469414,
-0.025105351582169533,
-0.00820158515125513,
-0.04098356142640114,
0.03904900327324867,
0.07340378314256668,
-0.024226311594247818,
0.039646804332733154,
-0.00450615119189024,
-0.020923105999827385,
-0.023331044241786003,
-0.01416955329477787,
0.022225389257073402,
-0.0635199025273323,
-0.043838243931531906,
0.00846671313047409,
-0.015460393391549587,
0.006636261474341154,
-0.031498417258262634,
0.007189007010310888,
0.05674899369478226,
0.002071516355499625,
0.028624270111322403,
0.002654493320733309,
0.03384970873594284,
0.05139876902103424,
0.0022336244583129883,
-0.03579141944646835,
0.0067488038912415504,
-0.05951319634914398,
-0.015788376331329346,
0.020189877599477768,
-0.03222324699163437,
-0.03961552307009697,
0.004566952120512724,
0.3558669686317444,
-0.037145569920539856,
-0.011680117808282375,
-0.01370928343385458,
-0.03588245436549187,
-0.015573878772556782,
-0.015383003279566765,
-0.00884127989411354,
-0.0488252155482769,
-0.001587257138453424,
0.019706398248672485,
-0.01950039528310299,
-0.03704378753900528,
0.07572076469659805,
-0.08622182160615921,
-0.039838626980781555,
-0.01690000481903553,
-0.02395857311785221,
-0.04317443445324898,
0.03928738832473755,
0.011650609783828259,
-0.010012133046984673,
0.01536531187593937,
0.028295570984482765,
0.02605377323925495,
0.02382943406701088,
-0.04181148484349251,
-0.01606767065823078,
0.09580663591623306,
0.03084832802414894,
0.08620236068964005,
0.04418284818530083,
-0.023890940472483635,
-0.0461917370557785,
-0.012512916699051857,
0.005568267311900854,
-0.01736532710492611,
0.0056974212639033794,
0.0011116573587059975,
0.052423443645238876,
0.006729329936206341,
-0.031244978308677673,
-0.03517089784145355,
0.041484277695417404,
-0.005677546374499798,
-0.03249109908938408,
0.12126916646957397,
-0.007905876263976097,
0.008589087054133415,
0.0020412688609212637,
-0.031537014991045,
-0.011726978234946728,
0.049298010766506195,
-0.028764864429831505,
-0.013917221687734127,
-0.010616825893521309,
0.06289392709732056,
0.012406439520418644,
-0.0023227748461067677,
0.0022280339617282152,
0.02937464602291584,
-0.04426892474293709,
-0.024611037224531174,
-0.014523985795676708,
0.09242317080497742,
-0.052810538560152054,
-0.013058021664619446,
0.028862476348876953,
-0.008641316555440426,
0.03314603865146637,
-0.038413915783166885,
0.030956309288740158,
-0.0052259392105042934,
-0.005986771546304226,
-0.005988110788166523,
-0.024921029806137085,
-0.03809386491775513,
0.012717023491859436,
0.01645289734005928,
0.011189640499651432,
-0.014242409728467464,
0.03639358654618263,
0.025465786457061768,
-0.0695909708738327,
-0.013821792788803577,
0.022162672132253647,
-0.024258838966488838,
-0.042044393718242645,
-0.01602332852780819,
-0.05543795973062515,
0.03744502738118172,
-0.025080254301428795,
0.03302416577935219,
-0.03104635700583458,
0.07591690123081207,
-0.020763905718922615,
-0.016980977728962898,
-0.0901905745267868,
-0.04363612085580826,
-0.06046244502067566,
-0.03676029294729233,
0.1378488689661026,
0.0021163641940802336,
0.008145274594426155,
0.014623697847127914,
0.04162712022662163,
0.028970282524824142,
0.04437485709786415,
0.004200327210128307,
-0.006100651808083057,
0.0438992865383625,
-0.06314731389284134,
-0.0035598850809037685,
-0.035626351833343506,
0.005252084229141474,
-0.013195517472922802,
0.038604918867349625,
-0.013431286439299583,
0.022860268130898476,
-0.018690967932343483,
0.0314023420214653,
0.06140328571200371,
-0.02168920263648033,
-0.03880991414189339,
-0.322203129529953,
0.028983071446418762,
0.021497031673789024,
0.014159061945974827,
0.05683606490492821,
-0.01521939504891634,
-0.006765817757695913,
0.016191516071558,
-0.015223687514662743,
0.018633302301168442,
0.030215056613087654,
0.12629961967468262,
-0.04813849553465843,
-0.014070075936615467,
-0.04371047019958496,
0.0055175707675516605,
0.015996644273400307,
-0.0044433437287807465,
-0.03400837257504463,
0.011058242060244083,
-0.042984738945961,
-0.026119304820895195,
0.005801503546535969,
0.0441351979970932,
0.08127281814813614,
-0.021034592762589455,
0.1411447674036026,
-0.035905368626117706,
0.007723750080913305,
-0.007451089099049568,
0.00808953121304512,
0.028107764199376106,
0.005953185260295868,
-0.033042438328266144,
0.012582887895405293,
-0.036140717566013336,
0.039452098309993744,
0.011973722837865353,
0.005207771901041269,
-0.0027299209032207727,
0.012898481450974941,
0.06252191215753555,
0.030304603278636932,
-0.027067091315984726,
-0.07601840049028397,
0.03014492057263851,
-0.0005654236301779747,
-0.01576748862862587,
-0.004748838488012552,
-0.023243125528097153,
0.018531329929828644,
0.06543312966823578,
0.08922495692968369,
0.014827768318355083,
0.03437725082039833,
-0.0010468027321621776,
-0.03683651611208916,
-0.007247142493724823,
-0.048550765961408615,
-0.017680827528238297,
-0.0076667191460728645,
-0.04867943003773689,
0.008390240371227264,
-0.001683223294094205,
0.04230378940701485,
0.011827030219137669,
0.020163124427199364,
-0.011945804581046104,
0.076337531208992,
0.042414795607328415,
0.0178962554782629,
0.0985381230711937,
-0.0791054219007492,
0.05260267108678818,
0.08403591066598892,
-0.0053741042502224445,
0.05646374449133873,
-0.03944835439324379,
-0.07014545053243637,
0.03432530164718628,
-0.049653880298137665,
-0.022314632311463356,
-0.0030583871994167566,
-0.04772813618183136,
-0.03771234676241875,
-0.030025513842701912,
0.007851650938391685,
-0.012098351493477821,
0.06479139626026154,
0.018632978200912476,
-0.021349765360355377,
0.002505159704014659,
-0.009320773184299469,
-0.009784244000911713,
0.022633880376815796,
0.0263714287430048,
-0.2901882231235504,
-0.020780518651008606,
-0.0305852722376585,
0.0010234324727207422,
-0.023578710854053497,
-0.008233588188886642,
0.03449169173836708,
-0.06785082072019577,
-0.039949215948581696,
-0.025501800701022148,
-0.05107419937849045,
-0.019077984616160393,
0.09640832990407944,
-0.09146160632371902,
-0.04469069465994835,
0.00045948170009069145,
0.007509828079491854,
-0.04221871867775917,
0.01662610098719597,
-0.05526874586939812,
0.045593854039907455,
-0.0027228891849517822,
0.14271438121795654,
-0.08618974685668945,
-0.05525854229927063,
-0.011229561641812325,
0.05368930101394653,
0.010881956666707993,
-0.039471328258514404,
-0.001702985493466258,
-0.008496752008795738,
0.03660259768366814,
0.04488636553287506,
-0.07617443799972534,
0.012853340245783329,
0.10534361004829407,
-0.06370308995246887,
0.0038762905169278383,
0.00503655057400465,
-0.020798977464437485,
-0.0076921735890209675,
-0.017465444281697273,
-0.06837302446365356,
0.004700739402323961,
0.03476155921816826,
-0.029144911095499992,
-0.04539715126156807,
-0.02585786208510399,
0.03631771728396416,
0.05486098304390907,
-0.03090035729110241,
0.04179239645600319,
0.00899659562855959,
0.06051844730973244,
-0.022332580760121346,
0.00235317787155509,
-0.03559194505214691,
0.014912383630871773,
0.02721252292394638,
-0.036519329994916916,
-0.012297787703573704,
-0.012658555060625076,
-0.018795309588313103,
0.05434251204133034,
-0.015823164954781532
] |
PMID:18573
|
Autoxidation of N-hydroxyphenylalkylamines: the inhibitory effect of some anions on copper catalysed autoxidation of N-hydroxyphentermine.
|
The inhibitory effect of certain electrolytes and buffers on the copper catalysed autoxidation of N-hydroxyphentermine (2-hydroxylamino-2-methyl-1-phenylpropane) has been investigated. The presence of ions such as SO4(2-), Cl- or Br- markedly reduced the rate of oxidation. Phosphate and carbonate buffers had a similar effect with halides and phosphate buffers being the most inhibitory. The occurence of 2-methyl-2-nitro-1-phenylpropane and 2-methyl-1-phenylpropene-(1) as secondary oxidation products was also established.
|
Autoxidation of N-hydroxyphenylalkylamines: the inhibitory effect of some anions on copper catalysed autoxidation of N-hydroxyphentermine. The inhibitory effect of certain electrolytes and buffers on the copper catalysed autoxidation of N-hydroxyphentermine (2-hydroxylamino-2-methyl-1-phenylpropane) has been investigated. The presence of ions such as SO4(2-), Cl- or Br- markedly reduced the rate of oxidation. Phosphate and carbonate buffers had a similar effect with halides and phosphate buffers being the most inhibitory. The occurence of 2-methyl-2-nitro-1-phenylpropane and 2-methyl-1-phenylpropene-(1) as secondary oxidation products was also established.
|
[
-0.08400791883468628,
0.0069237519055604935,
0.0013942073564976454,
0.05580093339085579,
0.005096054635941982,
-0.03120124712586403,
0.05219488963484764,
0.030996091663837433,
-0.05116896331310272,
-0.07129371166229248,
0.03303049877285957,
0.011780628003180027,
0.05549042299389839,
0.05650635063648224,
-0.006141541060060263,
0.012688239105045795,
-0.01730680651962757,
-0.04317774996161461,
-0.02915244735777378,
-0.0195704884827137,
0.0007798584410920739,
0.008017204701900482,
0.00006327268056338653,
-0.011212131939828396,
0.0501328781247139,
0.07589183747768402,
0.020237043499946594,
0.023274580016732216,
-0.05173977464437485,
-0.18827538192272186,
0.04096577316522598,
0.03514305502176285,
0.020134631544351578,
-0.01717168092727661,
-0.03163910284638405,
0.0271805040538311,
-0.046804167330265045,
0.027515443041920662,
0.008896633051335812,
-0.016186548396945,
0.0014187293127179146,
0.04097883403301239,
-0.0005421338137239218,
0.04430098086595535,
0.05300943925976753,
0.021507050842046738,
0.016338352113962173,
-0.018923263996839523,
0.10619387030601501,
-0.01832471415400505,
-0.03562270849943161,
0.04767834395170212,
0.023593636229634285,
0.020613713189959526,
0.042926445603370667,
0.015991918742656708,
0.026667580008506775,
0.017744142562150955,
-0.028344400227069855,
0.010274619795382023,
0.05594222992658615,
0.059036362916231155,
-0.16099430620670319,
0.027559204027056694,
0.03976380079984665,
0.00967458076775074,
-0.022778118029236794,
0.026296120136976242,
0.0774109959602356,
0.07964766025543213,
-0.044639717787504196,
-0.004375997465103865,
-0.025408200919628143,
-0.032662440091371536,
0.030694779008626938,
0.01658518612384796,
0.020687207579612732,
-0.03749429062008858,
-0.008240079507231712,
0.0024673165753483772,
-0.0035422001965343952,
-0.012703612446784973,
-0.00504637835547328,
0.015914611518383026,
0.005875155795365572,
0.019354619085788727,
0.015630299225449562,
0.034504201263189316,
0.013170085847377777,
-0.055562280118465424,
-0.00001491299281042302,
-0.04791109636425972,
0.030028020963072777,
-0.015623821876943111,
-0.03867703303694725,
0.06255385279655457,
-0.006460142321884632,
-0.07667410373687744,
0.018812967464327812,
0.3820969760417938,
-0.017023758962750435,
0.012504915706813335,
-0.06547750532627106,
-0.03754865750670433,
0.0228948425501585,
-0.00832612905651331,
-0.027540192008018494,
-0.03874611109495163,
0.05986040458083153,
0.0056517235934734344,
0.07856420427560806,
0.0762893557548523,
0.035514991730451584,
-0.07648304104804993,
0.022378727793693542,
0.014305710792541504,
0.02029617503285408,
-0.029587944969534874,
0.000018845366867026314,
0.04332073777914047,
-0.001077030785381794,
0.01695244386792183,
0.07522597908973694,
0.05895952135324478,
-0.03393954783678055,
-0.010325977578759193,
-0.003900746814906597,
0.07817498594522476,
0.01129171997308731,
-0.014925509691238403,
-0.030097318813204765,
-0.005884581245481968,
0.005542976316064596,
0.022385533899068832,
-0.03745817020535469,
0.008942974731326103,
0.05400553345680237,
-0.06003054231405258,
0.038445211946964264,
0.01652442291378975,
-0.0257418192923069,
-0.04204351827502251,
-0.07962168753147125,
-0.1061956062912941,
-0.038871102035045624,
0.026992149651050568,
0.037056926637887955,
-0.0075875623151659966,
-0.030565187335014343,
-0.011622453108429909,
-0.02314099855720997,
0.0644131451845169,
-0.0676242783665657,
0.04607303813099861,
-0.05032167211174965,
0.04906761273741722,
0.01989898644387722,
-0.029492558911442757,
0.009964948520064354,
0.05285944417119026,
-0.10667039453983307,
-0.019166596233844757,
-0.0006377142854034901,
0.10452203452587128,
0.017760751768946648,
-0.024704819545149803,
-0.09240031987428665,
-0.015094252303242683,
-0.018583321943879128,
-0.006801309064030647,
0.001704250113107264,
0.006294547580182552,
-0.03860064968466759,
-0.008697773329913616,
-0.02763833850622177,
0.02738654613494873,
-0.006131459027528763,
-0.002874009544029832,
0.0018952081445604563,
-0.01732761785387993,
0.026947926729917526,
-0.003788094501942396,
0.01255536638200283,
0.014208105392754078,
0.05384916439652443,
-0.036233630031347275,
-0.03826586529612541,
-0.021162349730730057,
0.030572528019547462,
-0.007934103719890118,
0.002316418569535017,
-0.021981436759233475,
-0.06608739495277405,
0.03189265727996826,
-0.029249807819724083,
-0.025324499234557152,
-0.08804597705602646,
0.013554459437727928,
0.04944898933172226,
-0.05361944064497948,
0.07277154177427292,
0.02787039801478386,
-0.029661957174539566,
0.06160437688231468,
0.013974368572235107,
0.008080115541815758,
-0.04630809277296066,
0.008082695305347443,
0.0464000403881073,
-0.026451636105775833,
-0.033997658640146255,
-0.04689119756221771,
-0.01080232486128807,
0.008547442965209484,
0.004988567437976599,
0.006330884527415037,
-0.014133153483271599,
-0.027526702731847763,
0.07082977145910263,
0.008610213175415993,
0.04584800824522972,
-0.04131421446800232,
-0.045849673449993134,
-0.27983883023262024,
-0.02186068519949913,
-0.0230515506118536,
-0.0017205660697072744,
0.04710175469517708,
-0.10599005967378616,
0.020953834056854248,
-0.05635548382997513,
-0.00223917281255126,
-0.03060898929834366,
-0.017578670755028725,
0.058772798627614975,
-0.01779639720916748,
-0.029546869918704033,
0.029530128464102745,
-0.023124132305383682,
0.03724995627999306,
-0.04964799806475639,
0.0458340048789978,
-0.00012520980089902878,
0.03322513774037361,
-0.005738240201026201,
0.03779014199972153,
0.0024353889748454094,
0.02142941765487194,
-0.008288029581308365,
0.11161067336797714,
0.0184175968170166,
-0.03421179577708244,
-0.013845779933035374,
-0.030164236202836037,
0.060423240065574646,
-0.02164306491613388,
-0.09919676929712296,
-0.03350948169827461,
-0.06215488538146019,
0.00009464969480177388,
-0.0357767678797245,
-0.042621832340955734,
-0.03472078964114189,
-0.0029545475263148546,
0.018806057050824165,
0.013278249651193619,
-0.09561177343130112,
-0.01565791293978691,
-0.055674489587545395,
-0.03490700572729111,
-0.041121695190668106,
0.004633564502000809,
-0.01729675568640232,
0.05346943810582161,
0.06406442075967789,
0.028903013095259666,
-0.017821254208683968,
-0.0241823922842741,
-0.022655023261904716,
-0.09193228930234909,
0.052371829748153687,
-0.03548651933670044,
-0.04194197058677673,
-0.01860681362450123,
-0.0922747403383255,
0.0023241254966706038,
-0.007932650856673717,
0.040265873074531555,
-0.00578625313937664,
0.03754233568906784,
-0.0681426003575325,
0.02173200063407421,
0.07824338227510452,
0.0736631453037262,
0.15441679954528809,
-0.04689627140760422,
-0.006405321415513754,
0.02088361233472824,
0.017210956662893295,
-0.03258009999990463,
-0.0028253579512238503,
-0.04616912454366684,
-0.0036731064319610596,
-0.07455568760633469,
-0.005042766220867634,
0.0663774162530899,
0.027045737951993942,
0.006374803371727467,
0.02170282043516636,
0.02633860893547535,
-0.026268435642123222,
0.032534271478652954,
0.037547577172517776,
-0.005661274306476116,
0.03480909764766693,
-0.05411473661661148,
-0.011436543427407742,
0.03049355186522007,
0.08757491409778595,
-0.23987190425395966,
-0.04251175373792648,
0.015079188160598278,
-0.005674149375408888,
0.0020854466129094362,
0.01191925723105669,
0.005527849309146404,
-0.039085421711206436,
-0.017832394689321518,
0.05553644523024559,
-0.017267538234591484,
0.08148958534002304,
0.06300917267799377,
0.03562409058213234,
0.037667665630578995,
-0.02991819940507412,
0.023660654202103615,
0.014692624099552631,
-0.0010584150440990925,
-0.05276266485452652,
0.05278469994664192,
-0.005225633271038532,
0.12891151010990143,
0.041613247245550156,
-0.0476854033768177,
0.08721708506345749,
-0.0013203570852056146,
0.020125050097703934,
-0.026161979883909225,
0.016517506912350655,
-0.06394975632429123,
0.025269977748394012,
0.09510646015405655,
-0.028478968888521194,
0.04970179498195648,
0.04639311879873276,
-0.038499314337968826,
-0.05857628211379051,
-0.01249166950583458,
0.003875859547406435,
-0.01800929568707943,
-0.011853520758450031,
-0.11017835140228271,
-0.018658766523003578,
0.008199070580303669,
-0.0737740695476532,
0.002437352202832699,
-0.03821111097931862,
0.006097962614148855,
0.011699264869093895,
-0.01474633626639843,
-0.040381789207458496,
-0.0010773739777505398,
0.07210614532232285,
-0.008584932424128056,
0.029087403789162636,
-0.04924109950661659,
0.01390645932406187,
0.030924992635846138,
0.02819412760436535,
-0.00228267558850348,
0.013599559664726257,
-0.01987387053668499,
0.03777356818318367,
0.055440399795770645
] |
PMID:18574
|
Generated surface area measurement of disintegrating tablets.
|
The surface areas generated by the disintegration and dissolution of six commercial brands of phenylbutazone tablets B.P. 100 mg have been measured using a Model TA Coulter Counter. The graphs of surface area generated against time were all of the same shape and always reached a maximum value. The initial surface area increase was due to tablet disintegration and deaggregation and followed first order kinetics. A good correlation between the slope of this initial increase and disintegration time was found. The maximum surface area generated correlated at the probability level of better than 99-9% with the dissolution rat measured as t60. The subsequent decrease in surface area, after this maximum, was considered to be due to phenylbutazone dissolution and was also first order rate controlled.
|
Generated surface area measurement of disintegrating tablets. The surface areas generated by the disintegration and dissolution of six commercial brands of phenylbutazone tablets B.P. 100 mg have been measured using a Model TA Coulter Counter. The graphs of surface area generated against time were all of the same shape and always reached a maximum value. The initial surface area increase was due to tablet disintegration and deaggregation and followed first order kinetics. A good correlation between the slope of this initial increase and disintegration time was found. The maximum surface area generated correlated at the probability level of better than 99-9% with the dissolution rat measured as t60. The subsequent decrease in surface area, after this maximum, was considered to be due to phenylbutazone dissolution and was also first order rate controlled.
|
[
-0.01671823300421238,
0.001692216144874692,
0.023406680673360825,
-0.02202015183866024,
0.011695015244185925,
-0.03508889302611351,
0.017467612400650978,
0.05826377496123314,
-0.056831780821084976,
-0.02142244391143322,
-0.004695345181971788,
-0.062212396413087845,
0.020337192341685295,
0.05590387433767319,
-0.01921367086470127,
0.013381412252783775,
-0.009485852904617786,
-0.027104493230581284,
-0.02437431551516056,
-0.04343550652265549,
0.02545217238366604,
0.0015860490966588259,
0.020233580842614174,
-0.024474428966641426,
0.03766163811087608,
0.047984376549720764,
-0.041244883090257645,
0.008116479963064194,
-0.04136481136083603,
-0.21070152521133423,
0.04137076437473297,
0.05330370366573334,
0.0035242971498519182,
-0.032830964773893356,
-0.0007347002974711359,
0.02521417662501335,
-0.0664636418223381,
0.05281338840723038,
-0.008672740310430527,
0.0005119487177580595,
0.017241496592760086,
0.045875634998083115,
-0.03493360057473183,
0.01651107147336006,
0.025127993896603584,
-0.01822388917207718,
-0.11753141134977341,
0.035643503069877625,
0.04993938282132149,
0.014259161427617073,
0.06589147448539734,
-0.011080442927777767,
0.036531366407871246,
0.03921141475439072,
0.02094193547964096,
0.004535246174782515,
-0.03150549903512001,
0.03898651897907257,
0.004676216747611761,
0.05376346781849861,
0.04664764925837517,
0.0004288292257115245,
-0.16204339265823364,
0.041308946907520294,
0.06359147280454636,
-0.03986653313040733,
0.003230992006137967,
-0.062259361147880554,
0.02652672678232193,
0.06166883930563927,
-0.03236084058880806,
0.027991008013486862,
0.01619873195886612,
0.007473593112081289,
0.030054332688450813,
-0.017947372049093246,
0.0006760598625987768,
-0.004946392960846424,
-0.02118041180074215,
0.001279539312236011,
0.007778625003993511,
0.03558652475476265,
0.01241065189242363,
-0.018466472625732422,
-0.029296493157744408,
-0.03718706965446472,
0.05808677524328232,
0.03511405363678932,
0.039091117680072784,
0.04603796452283859,
0.03485885635018349,
0.008257493376731873,
-0.013720772229135036,
-0.024563509970903397,
0.0002428202424198389,
-0.006371628027409315,
-0.0205604899674654,
-0.009772679768502712,
0.026737237349152565,
0.37405502796173096,
-0.048395633697509766,
-0.027812449261546135,
-0.05001596733927727,
-0.014159118756651878,
-0.06379334628582001,
0.027487684041261673,
-0.0004952296731062233,
-0.022031472995877266,
-0.0427415557205677,
0.009166514500975609,
0.028223801404237747,
0.0003747984010260552,
-0.01179537083953619,
-0.06247768551111221,
-0.009820357896387577,
-0.057811446487903595,
0.033107925206422806,
0.02029288560152054,
-0.030491860583424568,
-0.011204957030713558,
0.013697097077965736,
0.04405830428004265,
0.00543213589116931,
-0.023971479386091232,
0.01601940393447876,
-0.007581464014947414,
-0.010799502022564411,
0.09416995942592621,
-0.012771636247634888,
-0.021002694964408875,
0.01902734488248825,
-0.0015389268519356847,
-0.05402630567550659,
0.03325071558356285,
-0.004008022136986256,
-0.007090693339705467,
0.0012489106738939881,
0.018957631662487984,
-0.0032441120129078627,
0.05357431247830391,
-0.03748157247900963,
-0.026179684326052666,
0.0010734943207353354,
-0.050907451659440994,
-0.030556324869394302,
0.1369839608669281,
0.008958864957094193,
0.02744464762508869,
0.015119061805307865,
-0.034309905022382736,
-0.012533827684819698,
0.01989651843905449,
-0.03621630370616913,
0.02953011356294155,
0.0066294921562075615,
0.012746668420732021,
0.01400944497436285,
-0.02764025330543518,
0.001835313392803073,
0.007231051567941904,
-0.03809858486056328,
-0.05050584673881531,
-0.00952743086963892,
0.12411990016698837,
-0.021276600658893585,
-0.024489186704158783,
-0.02188941277563572,
0.011927109211683273,
0.04560171812772751,
0.04009881615638733,
-0.025670573115348816,
-0.02500106766819954,
-0.044399723410606384,
-0.04226600378751755,
0.01531994715332985,
0.0022703649010509253,
0.02360118180513382,
-0.022982077673077583,
0.03313034027814865,
0.022340070456266403,
0.11482185870409012,
0.014002029784023762,
-0.030221065506339073,
-0.0023473354522138834,
-0.007961057126522064,
-0.03274449333548546,
-0.030291063711047173,
0.013885154388844967,
0.007929264567792416,
-0.007054557558149099,
-0.054847314953804016,
-0.03963857516646385,
-0.08100096881389618,
0.027580834925174713,
0.0016994616016745567,
-0.0027529995422810316,
-0.09075216948986053,
0.002211452927440405,
-0.006976551376283169,
-0.05149567499756813,
0.1344229131937027,
0.016067825257778168,
-0.04795396327972412,
0.03849524259567261,
0.08609941601753235,
0.08426118642091751,
0.05364438146352768,
-0.04965107887983322,
0.06761081516742706,
0.049975406378507614,
-0.006728162989020348,
0.001809119712561369,
0.01941526122391224,
-0.04794033616781235,
-0.02577139623463154,
0.0067558190785348415,
-0.0032103140838444233,
-0.03723733499646187,
-0.03046305477619171,
0.012907188385725021,
0.08129331469535828,
-0.03477933257818222,
-0.06026765704154968,
-0.3323393762111664,
0.07439170777797699,
0.045054398477077484,
0.02400115318596363,
0.06544044613838196,
-0.021536894142627716,
-0.0005150535725988448,
-0.05195637047290802,
0.01088525727391243,
-0.06502733379602432,
0.03505616635084152,
0.021308034658432007,
0.028216103091835976,
-0.016066482290625572,
-0.01599997468292713,
-0.033299073576927185,
0.009632261469960213,
0.07371308654546738,
-0.045394737273454666,
-0.045897092670202255,
0.014742918312549591,
-0.045913875102996826,
-0.0458613820374012,
0.015481658279895782,
0.035412587225437164,
0.028925800696015358,
0.1434047818183899,
-0.0669143795967102,
-0.029872644692659378,
0.002294567646458745,
-0.0010097430786117911,
0.01983429677784443,
-0.04238498583436012,
-0.006275765132158995,
0.002436479553580284,
-0.009116259403526783,
-0.004395386204123497,
-0.012394649907946587,
-0.03428608924150467,
-0.02646557427942753,
-0.04954088106751442,
0.008572227321565151,
0.04033207893371582,
-0.0820213258266449,
-0.01813507080078125,
-0.013236979953944683,
-0.057468123733997345,
-0.031458280980587006,
-0.004074656404554844,
0.0518774576485157,
-0.002922546584159136,
-0.003386379685252905,
0.015247030183672905,
-0.015999119728803635,
0.03876921907067299,
-0.03972656652331352,
0.01763221062719822,
0.05113416910171509,
-0.05844252184033394,
-0.058814097195863724,
-0.02227036841213703,
0.039803504943847656,
0.04869229346513748,
-0.01587243564426899,
-0.01655895635485649,
0.01880490966141224,
0.006400314625352621,
-0.024269025772809982,
0.0462154857814312,
0.012286754325032234,
0.0378824844956398,
0.1401260793209076,
-0.03993219882249832,
-0.0179457850754261,
0.05770816281437874,
-0.04479854553937912,
0.005863060709089041,
-0.004034122917801142,
-0.07799866795539856,
-0.012311986647546291,
-0.028586694970726967,
0.0012282499810680747,
0.041017744690179825,
-0.014177635312080383,
0.00048661252367310226,
0.03316190093755722,
-0.02678092196583748,
-0.0395544096827507,
0.043995946645736694,
0.03584364429116249,
-0.01788218878209591,
0.04507462680339813,
-0.024109547957777977,
-0.002313540782779455,
0.031100640073418617,
0.014747648499906063,
-0.29455310106277466,
0.003324712160974741,
0.018613984808325768,
0.037695638835430145,
-0.016201158985495567,
-0.023041702806949615,
0.05655088275671005,
0.05696249380707741,
-0.01702822931110859,
-0.019954189658164978,
-0.0488077849149704,
-0.004487569909542799,
0.02210303023457527,
-0.04232976958155632,
-0.0236983522772789,
-0.01707102544605732,
0.038026634603738785,
-0.04800376668572426,
-0.0031792023219168186,
-0.027608349919319153,
0.05275838077068329,
-0.02502024918794632,
0.15111002326011658,
0.039230380207300186,
-0.032936643809080124,
0.030032671988010406,
0.04125339910387993,
-0.006393791176378727,
0.049410004168748856,
-0.02331291325390339,
-0.050490666180849075,
0.04415692761540413,
0.026193492114543915,
0.017564542591571808,
0.028187302872538567,
0.08471181243658066,
-0.04247092083096504,
-0.025843046605587006,
-0.007307501509785652,
-0.015421181917190552,
0.02013135701417923,
-0.07219558209180832,
-0.014399110339581966,
0.04704054817557335,
0.05467784032225609,
-0.043335746973752975,
-0.03898538649082184,
-0.04104059562087059,
0.06721305102109909,
-0.012438321486115456,
-0.019459707662463188,
-0.02170507237315178,
-0.01580609753727913,
-0.019140709191560745,
0.026226239278912544,
0.03451963886618614,
-0.030796613544225693,
0.018950315192341805,
0.054333873093128204,
-0.023297157138586044,
0.03324873000383377,
-0.03059750236570835,
0.027826858684420586,
0.004492337815463543,
0.022627810016274452
] |
PMID:18591
|
Hydrolysis and epimerization kinetics of hetacillin in aqueous solution.
|
Methods were developed for quantitating epimerization to epihetacillin and hydrolysis to ampicillin in the alkaline degradation of hetacillin, and both rates in deuterium oxide at 35 degrees and in water at various temperatures were determined. In each case, plots of log k for the epimerization against pH or pD yielded straight lines with a positive slope, which verified the first-order dependence on the hydroxide ion or deuteroxide ion. The activation energy of the epimerization process was 21.2 kcal/mole. In aqueous solution at high pH, epimerization rather than conversion to ampicillin represents a major pathway of hetacillin degradation, although the beta-lactam ring of the hetacillin molecule is highly resistant to attack by the hydroxide ion.
|
Hydrolysis and epimerization kinetics of hetacillin in aqueous solution. Methods were developed for quantitating epimerization to epihetacillin and hydrolysis to ampicillin in the alkaline degradation of hetacillin, and both rates in deuterium oxide at 35 degrees and in water at various temperatures were determined. In each case, plots of log k for the epimerization against pH or pD yielded straight lines with a positive slope, which verified the first-order dependence on the hydroxide ion or deuteroxide ion. The activation energy of the epimerization process was 21.2 kcal/mole. In aqueous solution at high pH, epimerization rather than conversion to ampicillin represents a major pathway of hetacillin degradation, although the beta-lactam ring of the hetacillin molecule is highly resistant to attack by the hydroxide ion.
|
[
-0.09492689371109009,
-0.027838408946990967,
-0.0096731623634696,
-0.010768350213766098,
0.055437758564949036,
-0.05796903371810913,
-0.0035636790562421083,
0.011493140831589699,
-0.06302567571401596,
0.028452057391405106,
0.016938304528594017,
-0.05692725628614426,
-0.04392407462000847,
-0.0004897799808532,
-0.024493178352713585,
-0.012786634266376495,
-0.052578628063201904,
0.010952359065413475,
-0.011604602448642254,
-0.0009283923427574337,
-0.017445789650082588,
-0.052359916269779205,
0.03999332711100578,
0.018718909472227097,
0.046302665024995804,
0.06136533245444298,
0.050111595541238785,
0.027015799656510353,
-0.07887998968362808,
-0.2417534589767456,
0.04544719308614731,
-0.015090259723365307,
0.0073097869753837585,
0.014998993836343288,
0.006622073706239462,
0.06747142970561981,
-0.018810056149959564,
0.020453961566090584,
-0.04054131731390953,
0.008365225046873093,
0.0528440922498703,
0.057818543165922165,
-0.006700287573039532,
0.018366172909736633,
0.014051816426217556,
0.024973880499601364,
-0.07081776857376099,
0.024355918169021606,
0.09350696951150894,
0.02078525722026825,
-0.05025491863489151,
0.009266015142202377,
0.024173814803361893,
0.03843502700328827,
0.03785427659749985,
0.026797998696565628,
0.08294905722141266,
0.059759218245744705,
0.012861089780926704,
-0.027712730690836906,
0.013182070106267929,
0.0038165657315403223,
-0.14063404500484467,
0.05814896896481514,
-0.0007402282790280879,
0.00892139133065939,
0.026254083961248398,
-0.01613958552479744,
0.024838268756866455,
0.0693005919456482,
-0.028587501496076584,
-0.015324657782912254,
0.004412388429045677,
0.013250313699245453,
0.01106509380042553,
-0.04572565108537674,
-0.043686795979738235,
-0.032877035439014435,
0.038112103939056396,
-0.0015204540686681867,
-0.012822598218917847,
0.000890171155333519,
0.02168404683470726,
-0.0014899719972163439,
0.05597937852144241,
-0.007722225971519947,
0.04284970089793205,
-0.013914057053625584,
0.05916840583086014,
0.020219016820192337,
0.05200187861919403,
0.023005973547697067,
0.022719115018844604,
-0.03932953253388405,
-0.06072694808244705,
0.038572583347558975,
0.03620858117938042,
0.02264968492090702,
0.018231255933642387,
0.3924480080604553,
-0.0776970237493515,
-0.020837225019931793,
-0.04607801511883736,
-0.042028799653053284,
-0.000877977698110044,
0.0024870012421160936,
-0.020885465666651726,
-0.05606682598590851,
0.021021712571382523,
-0.016076399013400078,
0.0051542483270168304,
0.03189088776707649,
0.04670585319399834,
-0.09744233638048172,
0.024781392887234688,
0.018332604318857193,
0.03116977959871292,
-0.0023847930133342743,
-0.024310261011123657,
-0.017741339281201363,
0.01523693185299635,
-0.02914511412382126,
-0.0014111450873315334,
-0.009624595753848553,
0.021121501922607422,
0.016829630360007286,
-0.031490497291088104,
0.049276262521743774,
-0.0014410819858312607,
0.018105825409293175,
0.015249290503561497,
-0.03857848420739174,
-0.02310960181057453,
-0.021571246907114983,
-0.0026477857027202845,
-0.010114569216966629,
0.03458724170923233,
-0.023614536970853806,
0.012284474447369576,
0.017154758796095848,
-0.08932917565107346,
-0.032732605934143066,
-0.04760405048727989,
-0.09268631041049957,
-0.019911989569664,
0.0943952202796936,
-0.004225253593176603,
-0.021972855553030968,
-0.03034028969705105,
-0.027396410703659058,
-0.04520415514707565,
0.04302343726158142,
-0.055778712034225464,
0.025357350707054138,
0.017940955236554146,
-0.0013163205003365874,
0.013379367999732494,
-0.05128141865134239,
-0.043043456971645355,
-0.035614028573036194,
-0.08039636164903641,
0.0059402864426374435,
-0.03043823502957821,
0.1519138365983963,
-0.0069326008670032024,
-0.019983574748039246,
0.004286781419068575,
0.006862553767859936,
0.02973289228975773,
-0.00458031939342618,
0.01500127837061882,
0.0039878711104393005,
0.015637025237083435,
-0.040782470256090164,
-0.02136971428990364,
0.07720676064491272,
0.02475113235414028,
0.010536085814237595,
-0.002545164665207267,
-0.004149255808442831,
0.05758902058005333,
0.054135799407958984,
0.0005416438798420131,
0.059590596705675125,
0.07149595767259598,
-0.05093221738934517,
-0.016322221606969833,
-0.008111381903290749,
0.013169302605092525,
0.062069181352853775,
-0.013487965799868107,
0.008737612515687943,
-0.012833205983042717,
0.04387756809592247,
-0.0004946324042975903,
-0.015460586175322533,
-0.05906243994832039,
0.037839751690626144,
-0.018625076860189438,
0.007188868708908558,
0.07832435518503189,
0.013679982163012028,
0.0015977075090631843,
0.039467599242925644,
0.053307030349969864,
0.015440923161804676,
0.015545984730124474,
0.0013434715801849961,
0.03850197419524193,
-0.009675874374806881,
-0.09011255949735641,
-0.003022408578544855,
-0.05489720404148102,
-0.041877735406160355,
-0.023206809535622597,
0.0026239845901727676,
-0.014290830120444298,
0.05136856809258461,
0.00264453561976552,
0.0091401943936944,
0.06617067009210587,
-0.04612702131271362,
-0.07916449755430222,
-0.2585948407649994,
0.024684591218829155,
-0.03135520964860916,
-0.036114610731601715,
0.08525414764881134,
-0.07600420713424683,
-0.02092829905450344,
-0.0780327245593071,
0.03291727602481842,
-0.06984584033489227,
0.021643610671162605,
0.0012706138659268618,
0.004342647735029459,
0.006473969202488661,
-0.02163873426616192,
0.0239412784576416,
0.037561289966106415,
0.007739550434052944,
0.058884862810373306,
0.0015370292821899056,
-0.012552970089018345,
-0.012435225769877434,
0.021475208923220634,
0.040382739156484604,
0.0104693453758955,
-0.02263621985912323,
0.07934621721506119,
0.03654543682932854,
0.0323624461889267,
-0.0024666080716997385,
-0.02315114624798298,
0.02177615463733673,
-0.03997652605175972,
-0.08018411695957184,
-0.04705097898840904,
-0.037745170295238495,
0.04930269718170166,
-0.0379716232419014,
0.029700230807065964,
-0.06188039109110832,
-0.04365872964262962,
0.02716996520757675,
-0.031226912513375282,
-0.089998260140419,
-0.021219560876488686,
-0.015276488848030567,
0.0002483130374457687,
0.0871184691786766,
-0.021660419180989265,
-0.011027038097381592,
0.031092019751667976,
-0.031702641397714615,
0.06106136366724968,
-0.01866677589714527,
-0.02077980898320675,
-0.032029151916503906,
-0.015865039080381393,
0.06366686522960663,
-0.0800965279340744,
-0.004344083368778229,
0.013318785466253757,
-0.038658760488033295,
-0.0025156133342534304,
0.039738211780786514,
0.013085766695439816,
0.0010728318011388183,
0.0010006303200498223,
-0.010609813034534454,
0.011761014349758625,
-0.02961130626499653,
0.016520682722330093,
0.1038588434457779,
-0.06305176764726639,
0.004459996707737446,
0.04868853837251663,
-0.027474770322442055,
-0.029924985021352768,
-0.030656127259135246,
-0.0764964371919632,
-0.012594197876751423,
-0.02155928686261177,
-0.027045076712965965,
0.01876169815659523,
-0.046008091419935226,
0.012234248220920563,
0.01774076372385025,
-0.010864422656595707,
0.041546110063791275,
-0.010975190438330173,
0.01857566647231579,
-0.029270587489008904,
0.013950377702713013,
-0.021787310019135475,
-0.01118034403771162,
0.048009686172008514,
0.0695134773850441,
-0.2960399389266968,
-0.027139395475387573,
0.06009059026837349,
-0.010159246623516083,
-0.0028867225628346205,
0.010725169442594051,
0.06323275715112686,
-0.02458379417657852,
-0.053838592022657394,
0.0591713972389698,
-0.029766619205474854,
0.03259992226958275,
0.036485906690359116,
-0.002086613792926073,
0.03328465297818184,
0.013862481340765953,
0.0012998540187254548,
-0.05855945125222206,
0.004824340343475342,
-0.030330443754792213,
0.032900694757699966,
-0.008670566603541374,
0.1520545482635498,
-0.0466979444026947,
-0.01998082548379898,
0.06339943408966064,
0.026143651455640793,
0.07391978055238724,
0.02767631784081459,
0.022987281903624535,
-0.03948063775897026,
0.1027979627251625,
0.0612805150449276,
-0.036139022558927536,
0.09711504727602005,
0.015008500777184963,
-0.0018181191990152001,
-0.00344295846298337,
0.008233292028307915,
0.0026498690713196993,
-0.009603384882211685,
-0.03822891786694527,
-0.025372853502631187,
-0.03472154214978218,
0.03538580238819122,
-0.047132860869169235,
-0.029529428109526634,
-0.003222324186936021,
0.013040559366345406,
-0.029471687972545624,
-0.06623837351799011,
-0.018739381805062294,
0.01175457052886486,
0.07517106831073761,
0.02563152089715004,
0.05963282659649849,
-0.005687872413545847,
0.0034801154397428036,
0.008795230649411678,
-0.014626914635300636,
0.008916170336306095,
-0.021175000816583633,
-0.06378798186779022,
0.04212822765111923,
0.016737114638090134
] |
PMID:18592
|
Actions of mescaline on isolated rat atria.
|
Mescaline, in concentrations of 5 x 10(-4) and 1 x 10(-3) M, produced negative chronotropic and positive inotropic responses in isolated, spontaneously beating rat atria. In tissues driven at a constant rate, the inotropic response was diminished greatly, indicating that the increment in the force of contraction was secondary to the reduction in rate. These chronotropic and inotropic responses were not altered consistently by pretreatment with the histamine antagonists chlorpheniramine and metiamide.
|
Actions of mescaline on isolated rat atria. Mescaline, in concentrations of 5 x 10(-4) and 1 x 10(-3) M, produced negative chronotropic and positive inotropic responses in isolated, spontaneously beating rat atria. In tissues driven at a constant rate, the inotropic response was diminished greatly, indicating that the increment in the force of contraction was secondary to the reduction in rate. These chronotropic and inotropic responses were not altered consistently by pretreatment with the histamine antagonists chlorpheniramine and metiamide.
|
[
-0.06847191601991653,
-0.006640616338700056,
0.025995373725891113,
0.07267304509878159,
-0.023463429883122444,
-0.0030277410987764597,
0.07964378595352173,
0.031136155128479004,
-0.052130211144685745,
-0.010762176476418972,
0.00401249248534441,
-0.030543923377990723,
-0.04240575432777405,
0.005768420174717903,
-0.00921289250254631,
-0.028947863727808,
0.05748012661933899,
-0.013071516528725624,
-0.06968662887811661,
0.017377089709043503,
-0.06228522211313248,
0.010837576352059841,
0.015650674700737,
-0.014654606580734253,
0.033460259437561035,
-0.005443294998258352,
-0.035001058131456375,
-0.012172339484095573,
-0.05119257792830467,
-0.18735231459140778,
0.009929317981004715,
0.03779878094792366,
0.0016578728100284934,
-0.06703459471464157,
0.04108733683824539,
-0.03387276083230972,
-0.00003149008989566937,
0.02637277916073799,
0.008416054770350456,
0.06523548066616058,
0.033067889511585236,
-0.006799895316362381,
0.028460990637540817,
-0.002928235800936818,
-0.005928941071033478,
0.031473398208618164,
0.012233506888151169,
-0.006849030032753944,
0.08226316422224045,
-0.002182300668209791,
0.017330119386315346,
0.028255904093384743,
-0.006343803368508816,
0.020173393189907074,
0.033438172191381454,
-0.02427927777171135,
0.013036425225436687,
0.02468654327094555,
0.06416068971157074,
-0.03036479465663433,
0.030966361984610558,
0.05191592872142792,
-0.13408662378787994,
0.019771384075284004,
0.031034085899591446,
0.04751536250114441,
-0.01879320666193962,
0.045268137007951736,
-0.007859342731535435,
0.0836058035492897,
-0.04606553167104721,
0.0065005808137357235,
0.027251986786723137,
-0.008246174082159996,
0.018538860604166985,
-0.01686902716755867,
-0.017784057185053825,
-0.023884126916527748,
0.0028164891991764307,
0.002516532316803932,
0.05001814290881157,
-0.02761833555996418,
-0.026803070679306984,
-0.027845950797200203,
0.03463931754231453,
0.019718505442142487,
0.007840150035917759,
0.015371933579444885,
-0.0005086634773761034,
-0.044255830347537994,
-0.0064527313224971294,
-0.07609573751688004,
-0.007983271032571793,
-0.009436850436031818,
-0.04217066988348961,
-0.005240559112280607,
-0.018352095037698746,
0.018031666055321693,
-0.049867887049913406,
0.35360008478164673,
0.0247096735984087,
0.014009413309395313,
-0.08334220200777054,
-0.002287294017150998,
-0.01032928004860878,
-0.011378200724720955,
0.006867569871246815,
0.010996348224580288,
0.0009309482993558049,
0.07823071628808975,
0.05979561805725098,
-0.0016591456951573491,
0.0007874069851823151,
-0.015943001955747604,
0.0011743323411792517,
-0.06221379339694977,
0.04332514479756355,
-0.001893426408059895,
0.029853565618395805,
0.06580693274736404,
-0.005006160121411085,
-0.04527030140161514,
0.009893050417304039,
-0.07051552832126617,
0.06191101670265198,
-0.017205961048603058,
-0.026728635653853416,
0.10336466878652573,
0.0607348307967186,
-0.02737666293978691,
0.01524567510932684,
-0.027900904417037964,
-0.010352923534810543,
0.03360209986567497,
-0.015991689637303352,
-0.035249825567007065,
-0.004755268804728985,
0.013940976932644844,
-0.0010943972738459706,
-0.0005311219138093293,
-0.0662444680929184,
-0.0574546717107296,
0.015279903076589108,
-0.09591586887836456,
-0.04402681067585945,
0.11183743178844452,
0.04002721235156059,
0.022612620145082474,
-0.038251131772994995,
0.056798145174980164,
-0.02780388668179512,
0.031090129166841507,
-0.041759710758924484,
0.03950393944978714,
-0.028606150299310684,
0.03516095131635666,
-0.042601730674505234,
-0.036327261477708817,
0.006929520983248949,
-0.006539373192936182,
-0.09544442594051361,
-0.021522749215364456,
0.017059985548257828,
0.09867750108242035,
-0.0029564511496573687,
-0.0019730068743228912,
-0.04913469776511192,
0.06364118307828903,
-0.000645499094389379,
0.010319643653929234,
-0.0014906827127560973,
-0.007220124825835228,
0.017972534522414207,
-0.023877162486314774,
-0.007983908988535404,
-0.029816988855600357,
0.06406494975090027,
-0.03740563243627548,
0.04661630094051361,
0.0036164838820695877,
0.02208647131919861,
-0.0026245415210723877,
0.012606004253029823,
-0.0051877922378480434,
-0.02227332442998886,
0.009183316491544247,
-0.022991729900240898,
0.020976463332772255,
0.006324544548988342,
0.040413159877061844,
-0.03650231286883354,
-0.05819718912243843,
-0.08536754548549652,
0.06460783630609512,
-0.06939506530761719,
-0.008623935282230377,
-0.03065546602010727,
0.05112375319004059,
-0.017446672543883324,
0.01613277569413185,
0.08307965099811554,
-0.0324331596493721,
-0.04339392110705376,
0.0685768574476242,
0.03301161527633667,
0.10222617536783218,
-0.03745168447494507,
0.01025017537176609,
0.0077553801238536835,
-0.026305928826332092,
0.0019401811296120286,
-0.009336470626294613,
0.056147269904613495,
-0.00824400782585144,
-0.08421932905912399,
0.010603654198348522,
-0.008408837951719761,
-0.04393980652093887,
0.04186701774597168,
0.002882918808609247,
0.05109209939837456,
-0.03808540105819702,
-0.05611054599285126,
-0.27468183636665344,
-0.046747323125600815,
0.03552769497036934,
-0.026937859132885933,
0.06723897159099579,
0.0020433703903108835,
-0.0015705646947026253,
-0.07520117610692978,
0.0038176875095814466,
-0.04223842918872833,
0.0403924323618412,
0.08634443581104279,
-0.046687547117471695,
-0.04788989946246147,
-0.01972072757780552,
0.01378797460347414,
0.026500612497329712,
-0.035296548157930374,
0.030301032587885857,
0.0004632736963685602,
0.05883920565247536,
0.01495504379272461,
-0.06259295344352722,
0.029786914587020874,
0.02606968767940998,
-0.0033265501260757446,
0.11133098602294922,
-0.0005496444646269083,
-0.05024159699678421,
-0.02162286452949047,
0.015335253439843655,
0.03588780760765076,
-0.029449744150042534,
-0.06100543588399887,
-0.014279316179454327,
-0.018142100423574448,
0.056767065078020096,
-0.021346716210246086,
0.00914012175053358,
0.02551492489874363,
-0.07879361510276794,
0.011287524364888668,
0.0525229312479496,
-0.02297515608370304,
-0.0978456363081932,
-0.013366518542170525,
-0.043874386698007584,
0.05361326411366463,
-0.013324487023055553,
-0.04382054880261421,
0.041450995951890945,
-0.00323082460090518,
-0.018873486667871475,
-0.01582682505249977,
0.06694076955318451,
0.03817521035671234,
-0.08293513208627701,
-0.0006418953416869044,
-0.04531500115990639,
-0.02170361578464508,
0.011392918415367603,
0.03256327658891678,
0.012532372958958149,
-0.03623329848051071,
-0.06322705000638962,
0.010797953233122826,
0.049633000046014786,
0.006612439639866352,
0.030689409002661705,
-0.013585168868303299,
0.032623548060655594,
0.10427127033472061,
-0.009264880791306496,
0.05181590095162392,
0.0705747976899147,
0.007788078859448433,
-0.023410094901919365,
-0.023441962897777557,
-0.09672190248966217,
0.034769054502248764,
-0.021457813680171967,
-0.007257570046931505,
0.09513578563928604,
0.051595889031887054,
-0.034954968839883804,
0.001649731770157814,
-0.013947220519185066,
-0.009545418433845043,
0.07236781716346741,
0.03344230353832245,
0.04238276183605194,
0.08100728690624237,
0.0045264470390975475,
-0.0573589988052845,
0.014670024625957012,
-0.0037157887127250433,
-0.2998577654361725,
-0.01899106614291668,
0.03274146467447281,
0.01409555971622467,
-0.008032464422285557,
0.023785647004842758,
0.07276267558336258,
-0.018952185288071632,
-0.10663031786680222,
0.002477201633155346,
-0.049375224858522415,
0.04616926237940788,
0.02912921831011772,
-0.00949973426759243,
-0.04735482484102249,
0.03207184374332428,
0.02836882323026657,
0.04258011654019356,
0.026583079248666763,
-0.00973418727517128,
-0.002434974070638418,
0.0063752904534339905,
0.17068317532539368,
0.008470774628221989,
-0.0030432480853050947,
0.030972875654697418,
0.016924647614359856,
-0.004179313313215971,
0.001536153838969767,
0.027028651908040047,
0.009300675243139267,
-0.00760828098282218,
0.0939469188451767,
-0.07779469341039658,
0.029571885243058205,
-0.045852210372686386,
-0.07416775822639465,
0.0128695759922266,
-0.023625703528523445,
-0.002151971682906151,
-0.06613969802856445,
0.017960412427783012,
-0.015463137999176979,
-0.060578640550374985,
0.03838486596941948,
-0.08090168237686157,
-0.05166870728135109,
-0.01742853969335556,
-0.015576543286442757,
0.02029683068394661,
0.0038362976629287004,
-0.035533782094717026,
0.10992344468832016,
0.03601757436990738,
-0.01341316383332014,
-0.0003956495493184775,
-0.019730834290385246,
-0.04954571649432182,
0.01705837808549404,
-0.005158134736120701,
-0.015270411968231201,
0.02221325784921646,
-0.016929006204009056,
0.0377533882856369,
0.04186441749334335
] |
PMID:18593
|
Electronic absorption spectra and protolytic equilibria of doxorubicin: direct spectrophotometric determination of microconstants.
|
The ground- and excited-state dissociation constants and the electronic absorption and fluorescence spectra of doxorubicin were investigated by spectrophotometry. A general method for the direct calculation of individual microscopic dissociation constants was derived using the spectrophotometric data obtained. It was concluded that the protonated amino sugar group is slightly more acidic than the phenolic group. The spectrophotometric data were analyzed, and the macro- and microconstants for the various equilibria was reported.
|
Electronic absorption spectra and protolytic equilibria of doxorubicin: direct spectrophotometric determination of microconstants. The ground- and excited-state dissociation constants and the electronic absorption and fluorescence spectra of doxorubicin were investigated by spectrophotometry. A general method for the direct calculation of individual microscopic dissociation constants was derived using the spectrophotometric data obtained. It was concluded that the protonated amino sugar group is slightly more acidic than the phenolic group. The spectrophotometric data were analyzed, and the macro- and microconstants for the various equilibria was reported.
|
[
-0.06711756438016891,
0.023180892691016197,
-0.0032792347483336926,
-0.03225881606340408,
0.02193419449031353,
0.005980220623314381,
0.06427141278982162,
0.026274602860212326,
-0.014250189997255802,
-0.04331405088305473,
0.00516163045540452,
-0.013813497498631477,
0.030410660430788994,
0.017353475093841553,
-0.02361794374883175,
-0.03458606079220772,
0.02864959090948105,
-0.04672152176499367,
-0.01838529296219349,
0.020975477993488312,
-0.03434918820858002,
-0.018495408818125725,
-0.020210886374115944,
-0.034611381590366364,
0.050944797694683075,
0.014000236988067627,
-0.026721851900219917,
0.012587708421051502,
-0.08242377638816833,
-0.21420040726661682,
0.014540540985763073,
0.0006744599086232483,
0.02710871212184429,
0.006722757592797279,
-0.006569895427674055,
-0.007254600524902344,
-0.016154145821928978,
-0.011055230163037777,
-0.029914651066064835,
-0.041290245950222015,
0.00567219452932477,
-0.009966220706701279,
0.005274343769997358,
0.04890633374452591,
0.0053798179142177105,
0.008231814950704575,
-0.0817074403166771,
0.023128144443035126,
0.07689740508794785,
0.013869988732039928,
0.013601229526102543,
-0.01068155188113451,
0.06414640694856644,
0.002141311066225171,
0.0001713395322440192,
0.017890973016619682,
-0.0012535606510937214,
0.02231290005147457,
0.031873490661382675,
0.0018318290822207928,
0.017461957409977913,
0.08139187097549438,
-0.10663921386003494,
0.06409946829080582,
0.024176575243473053,
-0.057730451226234436,
0.020231187343597412,
-0.032817620784044266,
0.05041993781924248,
0.021345382556319237,
-0.043983493000268936,
0.03425028175115585,
-0.006738861557096243,
0.017990892753005028,
-0.002107132924720645,
-0.04488545283675194,
0.028622087091207504,
-0.03197731077671051,
-0.046557266265153885,
-0.011723828501999378,
-0.003320304211229086,
0.04618525505065918,
-0.019177021458745003,
-0.024183237925171852,
0.00579586299136281,
0.02095024660229683,
0.01851501874625683,
-0.005867814179509878,
0.05531134083867073,
0.0653122141957283,
-0.0005457754014059901,
-0.01792372204363346,
-0.05524861440062523,
-0.04634781926870346,
-0.10939390957355499,
0.02333526499569416,
0.027547482401132584,
0.013408475555479527,
0.052497196942567825,
0.41002726554870605,
-0.012467840686440468,
0.0028172284364700317,
-0.04154350236058235,
-0.04744548350572586,
-0.03434830158948898,
-0.010854941792786121,
-0.00861987005919218,
-0.004665276501327753,
0.03538067638874054,
0.005523881874978542,
0.028325548395514488,
-0.013405868783593178,
0.06864766776561737,
-0.049099523574113846,
-0.023632680997252464,
0.023530667647719383,
0.0344325453042984,
-0.002869986928999424,
0.018626151606440544,
-0.04208185151219368,
-0.005165909416973591,
0.012102239765226841,
0.02573217637836933,
0.006216599140316248,
0.12082453817129135,
-0.04972032457590103,
-0.02937326952815056,
0.023469900712370872,
0.031214648857712746,
0.042069535702466965,
0.03411516547203064,
0.04374481365084648,
-0.02224660851061344,
-0.02046775445342064,
0.02481268160045147,
-0.022645555436611176,
0.03685464709997177,
0.007583480328321457,
0.01681964285671711,
0.01099773682653904,
-0.06066105514764786,
-0.0398511178791523,
-0.05827144533395767,
-0.03754395991563797,
-0.07672370225191116,
0.032972678542137146,
0.0192329753190279,
0.011519464664161205,
0.01898997835814953,
0.00932301301509142,
-0.028231222182512283,
0.052378665655851364,
-0.01909605599939823,
0.028044860810041428,
-0.02548191323876381,
0.07183676958084106,
-0.0029740913305431604,
-0.05490727722644806,
-0.02803679183125496,
0.02468714490532875,
-0.054813504219055176,
-0.02227046899497509,
-0.01613624021410942,
0.16406410932540894,
-0.029847431927919388,
-0.03313054144382477,
-0.010356882587075233,
0.021637776866555214,
-0.0013923738151788712,
-0.0255443062633276,
0.007779625244438648,
0.010491177439689636,
-0.03350936993956566,
0.004676417913287878,
0.020821264013648033,
0.0019600666128098965,
0.029090257361531258,
0.009608960710465908,
-0.01872212067246437,
0.016313426196575165,
0.0489082932472229,
0.03783126175403595,
-0.01778641901910305,
0.04509590566158295,
0.08606167882680893,
0.03771091625094414,
-0.05799878388643265,
0.058262575417757034,
-0.03317256644368172,
0.07586853206157684,
-0.04526578262448311,
-0.01969393901526928,
-0.06680308282375336,
0.07674513757228851,
-0.04495808482170105,
0.007722883950918913,
-0.06776392459869385,
-0.011048521846532822,
0.0084791649132967,
0.00017920552636496723,
0.11843150854110718,
0.008351707831025124,
-0.0322614386677742,
0.07024943828582764,
0.06895776838064194,
0.01648344285786152,
0.04018109664320946,
0.08612079173326492,
-0.0008246010402217507,
-0.04886067286133766,
-0.055806707590818405,
0.03278275951743126,
-0.07921399176120758,
-0.01999209076166153,
-0.033436231315135956,
-0.0216754749417305,
0.020636489614844322,
0.005709964316338301,
-0.0020426816772669554,
0.04927293583750725,
0.012240402400493622,
-0.05924169346690178,
-0.09190592169761658,
-0.29681310057640076,
0.02228168211877346,
0.009036255069077015,
-0.03477371856570244,
0.05510875582695007,
-0.027512909844517708,
0.017777569591999054,
-0.01631554402410984,
0.032518357038497925,
-0.04192394018173218,
-0.0014017801731824875,
0.06054248288273811,
-0.03172023966908455,
-0.05514070391654968,
-0.0027415992226451635,
-0.02308090217411518,
-0.04178829491138458,
-0.04245442897081375,
0.009481076151132584,
0.026928462088108063,
-0.014461202546954155,
-0.020420929417014122,
-0.0008317918400280178,
-0.003892035223543644,
0.060071513056755066,
-0.06223604455590248,
0.12589411437511444,
-0.0006212114822119474,
0.020669979974627495,
0.0035658215638250113,
-0.02420925162732601,
0.013276972807943821,
-0.056510258466005325,
-0.05539793521165848,
-0.0317998081445694,
-0.012064003385603428,
0.019797999411821365,
-0.033140916377305984,
0.029215218499302864,
-0.023417538031935692,
0.011624600738286972,
0.054042749106884,
0.014235583133995533,
-0.042505789548158646,
0.03211129084229469,
0.014334145933389664,
0.021075069904327393,
0.015551655553281307,
-0.007710696198046207,
-0.004858406260609627,
0.021177303045988083,
-0.001129084499552846,
-0.0007082592346705496,
-0.0270405113697052,
0.029293937608599663,
-0.04245365038514137,
-0.048668306320905685,
0.05309031903743744,
-0.04174245521426201,
-0.04742472246289253,
-0.02833736687898636,
-0.011377256363630295,
0.005282859317958355,
0.029655281454324722,
0.03293081372976303,
-0.022845381870865822,
-0.01683780364692211,
-0.04528595134615898,
0.060388173907995224,
-0.012545712292194366,
0.01586166024208069,
0.14396749436855316,
-0.05267006903886795,
0.029968857765197754,
0.08743322640657425,
0.031138941645622253,
0.020218854770064354,
-0.021301690489053726,
-0.043031804263591766,
0.027649179100990295,
-0.028963765129446983,
0.04429945349693298,
0.019100230187177658,
-0.05764486640691757,
-0.021044937893748283,
0.03878163546323776,
-0.020606596022844315,
-0.017336130142211914,
0.03893837705254555,
0.014389337971806526,
-0.0035959631204605103,
0.00483426358550787,
0.0016939261695370078,
-0.003584109013900161,
0.028922945261001587,
0.055269625037908554,
-0.24890531599521637,
0.0024589572567492723,
-0.02094751037657261,
0.07880261540412903,
-0.018792390823364258,
0.007432555314153433,
0.07011093199253082,
-0.058849774301052094,
-0.04592239484190941,
0.009291033260524273,
0.03283848613500595,
-0.01653296686708927,
0.016111284494400024,
-0.025454247370362282,
0.0009184175869449973,
-0.019240403547883034,
0.04183270037174225,
-0.07023556530475616,
-0.057110633701086044,
0.002944823820143938,
-0.019489692524075508,
0.0014258529990911484,
0.18035946786403656,
-0.05669024959206581,
-0.05878030136227608,
0.07319297641515732,
0.03392414003610611,
0.08557895570993423,
0.0018370331963524222,
0.00800170935690403,
-0.004588139709085226,
-0.009186627343297005,
0.025066900998353958,
-0.07483021169900894,
0.04409823939204216,
0.05570748820900917,
-0.01790376380085945,
-0.02895796112716198,
-0.0007106845150701702,
0.002816711086779833,
0.019883614033460617,
-0.01977492868900299,
-0.0479893833398819,
-0.05167070031166077,
0.08297615498304367,
-0.06741854548454285,
-0.03327031061053276,
-0.028320040553808212,
-0.03215273097157478,
-0.013846869580447674,
-0.04600588232278824,
0.014938896521925926,
0.001548887463286519,
0.0030546411871910095,
-0.02196921780705452,
-0.029066015034914017,
-0.038503002375364304,
-0.017745381221175194,
0.06522403657436371,
-0.015165305696427822,
0.0020858016796410084,
0.030947385355830193,
-0.025891797617077827,
0.0448894128203392,
0.053496088832616806
] |
PMID:18594
|
Solubility and ionization characteristics of phenytoin.
|
The solubility of phenytoin was determined in pH 7.4 and 5.4 phosphate buffers at five temperatures; in hydroalcoholic solutions, 0--4% methanol; and in pH 4.8--8.4 buffer solutions. From the temperature data, the enthalpy and entropy of solution of this nonideal system were calculated and were similar at both pH values. The data obtained from the buffer solutions were used to calculate the apparent dissociation constant, pKa', of phenytoin as 8.06. A GLC method with on-column methylation was used to quantitate phenytoin with 5-(p-methyl-phenyl)-5-phenylhydantoin as an internal standard. The assay uses chloroform of extraction of the drug from aqueous solutions. The ratio of peak heights was adjusted for weights of aqueous and organic layers, and results were calculated in micrograms per gram of sample and mole fraction of phenytoin. Although hydroalcoholic solutions enhanced drug solubility, there is a potentially significant disadvantage in using alcohol for clinical studies.
|
Solubility and ionization characteristics of phenytoin. The solubility of phenytoin was determined in pH 7.4 and 5.4 phosphate buffers at five temperatures; in hydroalcoholic solutions, 0--4% methanol; and in pH 4.8--8.4 buffer solutions. From the temperature data, the enthalpy and entropy of solution of this nonideal system were calculated and were similar at both pH values. The data obtained from the buffer solutions were used to calculate the apparent dissociation constant, pKa', of phenytoin as 8.06. A GLC method with on-column methylation was used to quantitate phenytoin with 5-(p-methyl-phenyl)-5-phenylhydantoin as an internal standard. The assay uses chloroform of extraction of the drug from aqueous solutions. The ratio of peak heights was adjusted for weights of aqueous and organic layers, and results were calculated in micrograms per gram of sample and mole fraction of phenytoin. Although hydroalcoholic solutions enhanced drug solubility, there is a potentially significant disadvantage in using alcohol for clinical studies.
|
[
-0.049216050654649734,
0.029155589640140533,
-0.023151328787207603,
-0.051084887236356735,
0.003319241339340806,
-0.03090846724808216,
0.027978116646409035,
0.03317806124687195,
-0.052569448947906494,
-0.01633860357105732,
-0.003205052576959133,
-0.0576382651925087,
0.06079459190368652,
0.01840587705373764,
-0.05107523128390312,
-0.029264992102980614,
0.05978735163807869,
-0.03886597976088524,
-0.0025721171405166388,
0.005136674270033836,
-0.011496394872665405,
-0.03950129449367523,
0.013529347255825996,
-0.021947257220745087,
0.07564681023359299,
0.029773524031043053,
0.029190847650170326,
0.02101067639887333,
-0.03945383056998253,
-0.22549088299274445,
0.03535335510969162,
0.0338258296251297,
0.02454991824924946,
-0.07588748633861542,
-0.01026401948183775,
0.030663950368762016,
-0.004394207615405321,
0.026002641767263412,
0.006092220079153776,
0.04017429053783417,
0.05707284435629845,
-0.005717664957046509,
-0.01857713982462883,
-0.02408202365040779,
0.05015644431114197,
-0.038114286959171295,
-0.06852543354034424,
0.031280383467674255,
0.13375377655029297,
0.006495238281786442,
-0.0016886942321434617,
0.045366331934928894,
0.023992273956537247,
0.061876989901065826,
-0.005923101678490639,
-0.015415474772453308,
0.042935632169246674,
0.009891988709568977,
0.009281250648200512,
0.0034180860966444016,
0.017748529091477394,
0.011564206331968307,
-0.11999649554491043,
0.0804748460650444,
-0.016007989645004272,
0.025676453486084938,
0.0078028966672718525,
0.027681615203619003,
0.015364859253168106,
0.029926754534244537,
-0.02877567708492279,
0.08364032208919525,
-0.056512605398893356,
0.027085134759545326,
0.02167629823088646,
-0.057947758585214615,
-0.01384561974555254,
-0.028758138418197632,
-0.007508486974984407,
0.011064654216170311,
-0.004557116888463497,
0.009581095539033413,
0.017989199608564377,
-0.0519535094499588,
0.019294498488307,
0.01557308528572321,
0.07886913418769836,
0.05205916613340378,
0.019764671102166176,
-0.012257477268576622,
0.01649210788309574,
-0.01581774279475212,
-0.05400007590651512,
-0.01830027997493744,
-0.020592590793967247,
0.06027244031429291,
-0.029070526361465454,
-0.04223845526576042,
0.021045148372650146,
0.32445257902145386,
-0.04350470378994942,
0.02533273957669735,
-0.08350615948438644,
-0.029307838529348373,
0.024030867964029312,
0.031651150435209274,
0.027701260522007942,
-0.03761287406086922,
0.038604434579610825,
-0.012524244375526905,
-0.014379872009158134,
0.04255237057805061,
0.061275601387023926,
-0.06468919664621353,
-0.04863307625055313,
-0.01502294559031725,
-0.006615576334297657,
-0.048678696155548096,
0.0396885871887207,
0.0065568056888878345,
0.013110779225826263,
-0.014802074991166592,
0.0329238697886467,
0.008965905755758286,
0.016288090497255325,
0.005517413839697838,
-0.0038432450965046883,
0.03716961294412613,
0.01953708752989769,
0.042108554393053055,
0.0009506308706477284,
-0.04600386694073677,
-0.01738015189766884,
-0.05357654392719269,
-0.030373308807611465,
-0.054996341466903687,
0.04538210481405258,
-0.030756400898098946,
0.00565628195181489,
0.047871097922325134,
-0.048225037753582,
-0.04673483967781067,
-0.023041989654302597,
-0.07337402552366257,
-0.1083434447646141,
0.13901589810848236,
-0.028673842549324036,
0.006143422331660986,
-0.013193025253713131,
-0.01728411763906479,
-0.052220482379198074,
0.04865347221493721,
-0.04163912311196327,
0.019159622490406036,
0.021274225786328316,
0.024848254397511482,
0.030152596533298492,
-0.031490299850702286,
-0.049007587134838104,
0.035771433264017105,
0.021953919902443886,
-0.0355040580034256,
-0.009841238148510456,
0.1490415632724762,
-0.03851686418056488,
0.002504464704543352,
0.01643473654985428,
-0.009791873395442963,
0.01801604963839054,
-0.0076893107034265995,
0.0399169884622097,
-0.0018814346985891461,
-0.0507999062538147,
0.03271840512752533,
-0.02999783493578434,
-0.031606271862983704,
0.011177466250956059,
-0.016295868903398514,
0.025035524740815163,
-0.009842943400144577,
0.04570309445261955,
0.049021851271390915,
0.004463495220988989,
0.028478404507040977,
0.07396776229143143,
-0.02821190096437931,
-0.016632499173283577,
-0.022170282900333405,
-0.0332806371152401,
0.07800652086734772,
-0.016918431967496872,
-0.011983293108642101,
-0.03397740051150322,
0.051333777606487274,
-0.01397059578448534,
0.030326159670948982,
-0.04253576695919037,
-0.024826498702168465,
0.0008358353516086936,
-0.031128332018852234,
0.1160200908780098,
0.059115562587976456,
-0.04669291526079178,
0.04231828451156616,
0.031263791024684906,
0.09961088001728058,
0.03728097304701805,
-0.0203844103962183,
0.0008328068652190268,
0.03572256118059158,
-0.013235538266599178,
0.008892194367945194,
-0.03810045123100281,
-0.03131267428398132,
-0.035937726497650146,
0.0015785141149535775,
0.02295876108109951,
-0.024032508954405785,
0.021301910281181335,
0.0007859781617298722,
0.054491885006427765,
-0.07983811944723129,
-0.02418125607073307,
-0.31702226400375366,
0.0355660580098629,
0.015143325552344322,
-0.05825197696685791,
0.12411970645189285,
-0.05156547948718071,
0.04175750911235809,
-0.007157198153436184,
0.007950643077492714,
-0.05913832038640976,
0.05010084807872772,
0.04915734753012657,
-0.012780241668224335,
-0.023271523416042328,
-0.0008663520566187799,
-0.03982361778616905,
0.02242143824696541,
0.011436570435762405,
0.048206668347120285,
0.002438743133097887,
-0.01622588001191616,
-0.030365364626049995,
0.022174129262566566,
0.03532133251428604,
0.07977237552404404,
0.01944143883883953,
0.08772027492523193,
-0.03015293926000595,
-0.016459405422210693,
-0.038117147982120514,
0.020876238122582436,
-0.0005469296011142433,
-0.03623427823185921,
-0.09138213843107224,
0.020107749849557877,
-0.030950896441936493,
0.0025158061180263758,
0.013196027837693691,
0.004046245943754911,
0.0076950485818088055,
-0.01715558022260666,
0.06207223981618881,
0.05143748223781586,
-0.10524237155914307,
-0.07243897020816803,
0.021097585558891296,
-0.017626101151108742,
-0.005400741472840309,
-0.006274568382650614,
0.028984032571315765,
0.02337215095758438,
0.04437750205397606,
0.06548044085502625,
-0.04244565591216087,
0.032060910016298294,
-0.01451644767075777,
-0.00981344748288393,
0.0450725220143795,
-0.011533834040164948,
-0.003960268571972847,
-0.021965770050883293,
-0.03356777876615524,
-0.0031310489866882563,
0.028535284101963043,
-0.021051883697509766,
-0.006613012868911028,
0.034015953540802,
-0.031059298664331436,
0.08942443132400513,
0.02152991108596325,
0.04487651214003563,
0.11803656816482544,
-0.03232854604721069,
0.044560931622982025,
0.047689422965049744,
0.010183635167777538,
0.017813552170991898,
-0.06013554707169533,
-0.056965503841638565,
0.018297690898180008,
-0.0574955940246582,
-0.005037867929786444,
0.03806266561150551,
-0.06944207102060318,
-0.027921948581933975,
0.028966674581170082,
-0.022545799612998962,
0.0251662228256464,
0.013018328696489334,
0.020291833207011223,
-0.02529369853436947,
-0.008888962678611279,
-0.02051297388970852,
-0.0422518216073513,
0.004940314218401909,
0.039736755192279816,
-0.280117005109787,
0.022291451692581177,
-0.039496924728155136,
-0.007492104545235634,
0.018020208925008774,
-0.041597817093133926,
0.02967747300863266,
-0.01664825528860092,
-0.09181416034698486,
0.030526988208293915,
-0.04165734723210335,
-0.012354540638625622,
0.05564040690660477,
-0.07110002636909485,
-0.026351872831583023,
-0.04116214066743851,
0.018141841515898705,
-0.10950476676225662,
-0.018858231604099274,
-0.028697723522782326,
0.0446491464972496,
-0.023786459118127823,
0.12158702313899994,
-0.01972896233201027,
-0.06567502021789551,
0.014146794565021992,
0.014306596480309963,
0.04739684239029884,
0.0071129878051579,
-0.0062800124287605286,
-0.02724766731262207,
0.04597246274352074,
0.023506512865424156,
0.011766602285206318,
0.06014450639486313,
0.08221148699522018,
-0.012484611012041569,
-0.040882837027311325,
0.03348463773727417,
0.05805732682347298,
0.03355064615607262,
-0.0643152967095375,
-0.04707039147615433,
-0.008166717365384102,
0.022898664698004723,
-0.05038817971944809,
-0.03991665691137314,
-0.04061514511704445,
0.002034557983279228,
0.0052071125246584415,
-0.040220074355602264,
-0.005235993769019842,
-0.015241551212966442,
0.010549230501055717,
0.008162187412381172,
0.06567417085170746,
-0.009497450664639473,
-0.008218557573854923,
0.03312761336565018,
-0.07172416150569916,
-0.034129172563552856,
-0.03720385208725929,
0.025722792372107506,
0.0455959215760231,
0.017556307837367058
] |
PMID:18595
|
Determination of acenocoumarol in plasma and urine by double radioisotope derivative analysis.
|
Acenocoumarol, to which a 14C-labeled internal standard has been added, is extracted at pH 4 into ethyl acetate-heptane (20:80 v/v), back-extracted into aqueous sodium hydroxide solution after solvent washing with a pH 7 buffer, and reextracted after acidification in the solvent mixture. It is then acetylated with 3H-acetic anhydride. The acenocoumarol acetyl derivative is separated from the metabolite derivatives by TLC, and its radioactivity is measured. The method is specific and sensitive to a concentration of 8 ng/ml.
|
Determination of acenocoumarol in plasma and urine by double radioisotope derivative analysis. Acenocoumarol, to which a 14C-labeled internal standard has been added, is extracted at pH 4 into ethyl acetate-heptane (20:80 v/v), back-extracted into aqueous sodium hydroxide solution after solvent washing with a pH 7 buffer, and reextracted after acidification in the solvent mixture. It is then acetylated with 3H-acetic anhydride. The acenocoumarol acetyl derivative is separated from the metabolite derivatives by TLC, and its radioactivity is measured. The method is specific and sensitive to a concentration of 8 ng/ml.
|
[
-0.0628708004951477,
0.02209351770579815,
-0.004294723272323608,
0.0005029152380302548,
0.05216275155544281,
-0.021132105961441994,
0.03986756503582001,
0.05301123857498169,
-0.10005242377519608,
-0.07009028643369675,
0.033579662442207336,
-0.03532550483942032,
0.008041654713451862,
0.033689193427562714,
-0.01949126087129116,
0.029457930475473404,
0.0029944677371531725,
-0.016803164035081863,
-0.02156531810760498,
0.023148206993937492,
-0.010536015965044498,
-0.01086410041898489,
0.014181447215378284,
0.005491806659847498,
0.07466212660074234,
0.042435064911842346,
0.01753350906074047,
-0.04177146777510643,
-0.02358601987361908,
-0.1555030196905136,
0.03777765855193138,
-0.014325500465929508,
-0.04255673289299011,
-0.057625703513622284,
-0.00570653798058629,
-0.013261750340461731,
-0.028648413717746735,
-0.0034147074911743402,
0.0281323604285717,
0.007281257770955563,
-0.03268439322710037,
-0.0549258291721344,
-0.047271694988012314,
-0.024370869621634483,
-0.047061070799827576,
0.0032817379105836153,
-0.03944924473762512,
0.0656658262014389,
0.12078465521335602,
0.04820123687386513,
-0.012001045048236847,
0.0785115584731102,
0.006560716312378645,
0.041048649698495865,
0.04812149330973625,
-0.052250463515520096,
0.02926400490105152,
0.025559699162840843,
-0.010630280710756779,
0.045320674777030945,
0.02940358594059944,
0.01574312336742878,
-0.16083118319511414,
0.0546090193092823,
-0.047159384936094284,
-0.020791232585906982,
-0.05258515477180481,
0.001637165085412562,
0.04877465218305588,
0.08696123957633972,
-0.03128640353679657,
0.011526934802532196,
-0.020897163078188896,
0.04360515996813774,
0.006860474124550819,
0.0011343730147928,
-0.0024411925114691257,
0.02417887933552265,
0.023213088512420654,
0.01629560813307762,
-0.016373544931411743,
0.05158769711852074,
-0.044671326875686646,
0.012873979285359383,
0.03262501209974289,
-0.0009680489893071353,
0.034752339124679565,
0.002185817575082183,
0.04671011492609978,
0.04553600773215294,
0.031676389276981354,
-0.02801116742193699,
-0.055544473230838776,
0.035941895097494125,
-0.012039565481245518,
-0.007982767187058926,
-0.0017981075216084719,
-0.01336293388158083,
-0.00005706934825866483,
0.3395492434501648,
-0.009507419541478157,
0.013531802222132683,
-0.08449256420135498,
-0.04353674128651619,
-0.05871212109923363,
-0.021908631548285484,
-0.026686539873480797,
-0.006361226551234722,
-0.045464131981134415,
0.04670775681734085,
0.011515581049025059,
0.049883659929037094,
0.027882596477866173,
-0.05884020775556564,
-0.00909850187599659,
-0.005280745681375265,
0.0243094339966774,
-0.020853357389569283,
0.028055215254426003,
0.021126067265868187,
0.05793456360697746,
-0.04649461433291435,
0.05169786512851715,
-0.052851103246212006,
0.07810011506080627,
0.0233926922082901,
0.014079027809202671,
0.044820334762334824,
0.06666713953018188,
0.09046271443367004,
0.04689887911081314,
-0.015605051070451736,
-0.030896330252289772,
-0.0017112691421061754,
-0.019126813858747482,
-0.009056949988007545,
0.047542259097099304,
0.015974758192896843,
0.055407535284757614,
0.05321607366204262,
-0.03271723911166191,
-0.0014091000193729997,
-0.05487591028213501,
-0.0409538634121418,
-0.07604213804006577,
0.15509448945522308,
-0.031068915501236916,
-0.023935329169034958,
-0.04328824579715729,
-0.022020209580659866,
0.0035753289703279734,
0.0654444471001625,
-0.05620265007019043,
0.03699510172009468,
-0.012953526340425014,
0.002013877499848604,
-0.00010640101390890777,
0.019794195890426636,
-0.02184567041695118,
0.025548342615365982,
-0.02896139770746231,
-0.0723550096154213,
-0.033388882875442505,
0.13044413924217224,
0.019657131284475327,
-0.03946317732334137,
0.0023388925474137068,
0.022397512570023537,
0.06897631287574768,
0.032613541930913925,
-0.006037816870957613,
0.026061784476041794,
-0.033433735370635986,
-0.02117764763534069,
-0.033529132604599,
0.033800434321165085,
-0.006313607562333345,
0.03712273761630058,
-0.05944640561938286,
-0.06548108160495758,
0.004640038590878248,
-0.0013515589525923133,
-0.03659354895353317,
0.0017530859913676977,
0.054489314556121826,
-0.0466410256922245,
-0.007857751101255417,
-0.028767919167876244,
0.013888977468013763,
0.04241248220205307,
-0.007080704439431429,
0.06712741404771805,
-0.07535174489021301,
0.001754051772877574,
-0.021384531632065773,
-0.028556810691952705,
-0.0568850003182888,
-0.015789780765771866,
0.006643973756581545,
0.004120646044611931,
0.1175541877746582,
0.003779381513595581,
0.0656101256608963,
0.011834464967250824,
0.04839375615119934,
0.06135242059826851,
0.0011569211492314935,
-0.02076384425163269,
-0.037803344428539276,
0.012605021707713604,
-0.02717871218919754,
-0.024260899052023888,
0.010999889113008976,
0.0024593700654804707,
-0.02745896205306053,
0.0251681599766016,
0.044746220111846924,
-0.01900673098862171,
-0.03707738220691681,
0.023156458511948586,
0.010025990195572376,
-0.005101179704070091,
-0.020737318322062492,
-0.3187335133552551,
-0.007977561093866825,
-0.010957127436995506,
0.009691842831671238,
0.03126987814903259,
-0.058542847633361816,
-0.015157729387283325,
-0.07984966784715652,
-0.01647452637553215,
-0.06266681849956512,
0.062028128653764725,
0.10188202559947968,
-0.0016778764547780156,
-0.02484201267361641,
-0.041843216866254807,
0.05811866372823715,
0.03945683315396309,
-0.059632979333400726,
0.015476168133318424,
-0.040191102772951126,
-0.029213858768343925,
-0.055598184466362,
-0.051889803260564804,
0.05796051025390625,
0.04408251494169235,
-0.053275950253009796,
0.10558073967695236,
-0.008394284173846245,
0.004857816267758608,
-0.05400283634662628,
0.017153048887848854,
-0.017181452363729477,
-0.002819736720994115,
-0.03191644325852394,
-0.0707840621471405,
-0.031174352392554283,
0.015048730187118053,
-0.05627045780420303,
0.04523473232984543,
0.015814512968063354,
0.033885400742292404,
-0.014089495874941349,
0.055267080664634705,
-0.0765460878610611,
-0.05760408565402031,
0.022679796442389488,
-0.024638604372739792,
0.03201746195554733,
-0.022733399644494057,
-0.006421665195375681,
0.05539853498339653,
0.03101421520113945,
0.013016393408179283,
-0.009415920823812485,
-0.06639240682125092,
0.026093123480677605,
-0.022062484174966812,
0.004638216458261013,
-0.0010504848323762417,
-0.023667488247156143,
-0.026986202225089073,
0.029053745791316032,
0.02440796233713627,
-0.017984051257371902,
0.02493714541196823,
0.019221963360905647,
0.027653038501739502,
-0.04577014967799187,
0.045252297073602676,
0.024114832282066345,
0.02850794419646263,
0.053894028067588806,
-0.06091703101992607,
0.059845298528671265,
0.029389046132564545,
0.04402714595198631,
-0.02672589011490345,
-0.01859442889690399,
-0.055412761867046356,
0.0398407019674778,
0.004583432804793119,
-0.08517591655254364,
0.023598143830895424,
0.007863515056669712,
-0.02720828913152218,
-0.022653678432106972,
0.030142225325107574,
-0.029043525457382202,
0.011645911261439323,
0.024396266788244247,
-0.004629271104931831,
0.04502808675169945,
-0.010000225156545639,
-0.07054752856492996,
-0.035995081067085266,
0.03866118937730789,
-0.28552865982055664,
0.0009207556722685695,
0.009883270598948002,
0.03207330033183098,
0.06113848462700844,
-0.0639880895614624,
0.03383368253707886,
-0.007918141782283783,
-0.05857747048139572,
0.025608476251363754,
-0.03347524628043175,
0.06444237381219864,
0.02254287339746952,
-0.02213948965072632,
-0.04096827656030655,
0.05109699070453644,
0.03302151337265968,
0.01134410034865141,
0.023153897374868393,
-0.02531345561146736,
0.06408853083848953,
-0.025458035990595818,
0.08418013155460358,
0.04329921677708626,
-0.07628890872001648,
0.09110236167907715,
0.017965374514460564,
0.011186306364834309,
-0.06208611652255058,
0.011898408643901348,
-0.02498682774603367,
0.018619254231452942,
0.01810365542769432,
-0.06247200444340706,
0.05470452830195427,
0.06515326350927353,
-0.011429399251937866,
0.021390309557318687,
-0.051148634403944016,
0.03587297722697258,
-0.03614070639014244,
-0.04121115058660507,
-0.055948276072740555,
-0.011962360702455044,
0.03314628452062607,
-0.11463865637779236,
-0.017000366002321243,
-0.03199871629476547,
-0.001062295981682837,
0.018994390964508057,
-0.004908731207251549,
0.02028079517185688,
0.009876650758087635,
-0.004340900108218193,
0.04270477965474129,
0.047199152410030365,
-0.03681178018450737,
-0.013620453886687756,
-0.023430638015270233,
-0.0008794745663180947,
-0.026197945699095726,
-0.006242383737117052,
0.010999566875398159,
0.08364179730415344,
0.08116570860147476
] |
PMID:18596
|
Kinetics of accumulation, efflux and the pharmacological effects of tritiated dihydroazapetine on the rabbit aorta.
|
Azepatine, a potent alpha adrenergic antagonist, was catalytically reduced with tritium and hydrogen gas to form dihydroazapetine. The pA2 azapetine was 7.9 whereas that of dihydroazapetine was 6.6, corresponding to a 20-fold decrease in potency. 3H-dihydroazapetine is accumulated into three kinetically distinct compartment in the denervated rabbit aorta. Likewise, efflux of the labeled antagonist occurs from three compartments. The rat constant for onset of alpha adrenoreceptor blockade is 3.15 min-1 which is nearly identical to the rate constant for entry of 3H-dihydroazapetine into a rapidly filling compartment (3.86 min-1) possibly representing the extracellular space. These data are consistent with the hypothesis that onset of alpha adrenoreceptor blockade by dihydroazapetine is diffusion limited.
|
Kinetics of accumulation, efflux and the pharmacological effects of tritiated dihydroazapetine on the rabbit aorta. Azepatine, a potent alpha adrenergic antagonist, was catalytically reduced with tritium and hydrogen gas to form dihydroazapetine. The pA2 azapetine was 7.9 whereas that of dihydroazapetine was 6.6, corresponding to a 20-fold decrease in potency. 3H-dihydroazapetine is accumulated into three kinetically distinct compartment in the denervated rabbit aorta. Likewise, efflux of the labeled antagonist occurs from three compartments. The rat constant for onset of alpha adrenoreceptor blockade is 3.15 min-1 which is nearly identical to the rate constant for entry of 3H-dihydroazapetine into a rapidly filling compartment (3.86 min-1) possibly representing the extracellular space. These data are consistent with the hypothesis that onset of alpha adrenoreceptor blockade by dihydroazapetine is diffusion limited.
|
[
-0.08069313317537308,
-0.05478339269757271,
0.018910929560661316,
0.03441387414932251,
-0.0075212628580629826,
-0.027261188253760338,
0.02385658025741577,
0.010748235508799553,
-0.016081079840660095,
-0.036091648042201996,
0.03935845568776131,
-0.06505643576383591,
0.0017470639431849122,
0.017681775614619255,
-0.024954669177532196,
0.0008407774730585515,
-0.020875131711363792,
-0.029086193069815636,
-0.024484725669026375,
-0.0331897996366024,
-0.04634907469153404,
-0.02224162220954895,
0.03870880603790283,
-0.029359303414821625,
0.03474508598446846,
0.06764811277389526,
0.027101609855890274,
-0.008184622973203659,
-0.017355481162667274,
-0.20148538053035736,
0.027897963300347328,
-0.03911251202225685,
-0.013048679567873478,
-0.0177728533744812,
-0.08002038300037384,
0.05994290113449097,
-0.0733584612607956,
0.0506538487970829,
-0.04464470222592354,
0.043661054223775864,
0.04476150497794151,
0.041035041213035583,
-0.042362064123153687,
0.00861602183431387,
-0.024920541793107986,
-0.003175310790538788,
-0.09029653668403625,
-0.011036255396902561,
0.09693247079849243,
-0.015099131502211094,
-0.013063007965683937,
-0.006982771214097738,
0.04165425896644592,
0.034372881054878235,
0.007020789664238691,
-0.02444293163716793,
0.04459139332175255,
0.0537596121430397,
-0.011648111045360565,
0.06659058481454849,
0.006918174214661121,
0.0282893069088459,
-0.14628760516643524,
0.04019602760672569,
0.06381282210350037,
-0.022975817322731018,
-0.022033851593732834,
0.015585724264383316,
0.0289541594684124,
0.03888290375471115,
-0.030255978927016258,
-0.0007723714224994183,
-0.015528412535786629,
0.006476015783846378,
-0.0002720256452448666,
-0.03988156095147133,
0.05070554465055466,
0.012830627150833607,
0.04413691908121109,
-0.04067513719201088,
0.034774862229824066,
-0.02101009711623192,
-0.03282248228788376,
-0.0451030470430851,
0.00398554652929306,
-0.022815942764282227,
0.05464634299278259,
0.01633404940366745,
-0.045376256108284,
-0.009497697465121746,
-0.021464882418513298,
-0.05649954080581665,
-0.0017232210375368595,
-0.0011419825023040175,
-0.04737531766295433,
0.015440535731613636,
0.004153326153755188,
0.014686239883303642,
-0.014938919804990292,
0.36625614762306213,
0.027873286977410316,
0.029976166784763336,
-0.038719289004802704,
-0.003536178031936288,
0.0068586841225624084,
-0.02100711688399315,
-0.03342117369174957,
-0.02504400722682476,
-0.02739497274160385,
0.015869470313191414,
0.08538606762886047,
-0.01923295482993126,
0.08057601004838943,
-0.05618506297469139,
-0.048186756670475006,
0.010155205614864826,
-0.00013121280062478036,
0.052569884806871414,
0.015099853277206421,
0.06304807215929031,
-0.01002420112490654,
-0.038317326456308365,
-0.027335746213793755,
-0.004153072368353605,
0.0626143217086792,
-0.005554684903472662,
-0.04039843752980232,
0.09656418114900589,
0.03960030525922775,
0.02164468914270401,
0.07830889523029327,
0.027532847598195076,
-0.028480902314186096,
0.01982179842889309,
0.04065663740038872,
0.006468492094427347,
0.04787386953830719,
-0.05608683452010155,
-0.002922277431935072,
-0.061627745628356934,
-0.049534719437360764,
-0.011709378100931644,
-0.03340911120176315,
-0.11389247328042984,
-0.05387258157134056,
0.19880658388137817,
0.04929633066058159,
-0.05537307634949684,
-0.056692980229854584,
-0.011368120089173317,
-0.009481876157224178,
0.03675119951367378,
0.00208999402821064,
-0.004599346779286861,
-0.0351736880838871,
0.0005913078784942627,
-0.03939943388104439,
0.03400907665491104,
-0.030739882960915565,
-0.031189996749162674,
-0.08133220672607422,
-0.06443275511264801,
-0.01254567876458168,
0.121169313788414,
0.0056887539103627205,
-0.008828390389680862,
-0.0573871023952961,
0.03799765557050705,
-0.020309176295995712,
0.05007198080420494,
-0.006439677905291319,
0.01621926948428154,
-0.05219811946153641,
0.02906421385705471,
-0.05256640166044235,
-0.044026799499988556,
-0.004916912876069546,
-0.013314192183315754,
0.0899994969367981,
-0.012006042525172234,
0.04961270093917847,
0.014109978452324867,
-0.01823357306420803,
0.009510223753750324,
0.06796427071094513,
0.018625233322381973,
-0.05128318816423416,
-0.002739058341830969,
-0.007205453235656023,
0.09991569817066193,
-0.021481024101376534,
0.023338304832577705,
-0.09275934845209122,
0.004993359558284283,
-0.0468984879553318,
-0.037268560379743576,
-0.03805186226963997,
0.04521945118904114,
-0.06191016361117363,
0.001706307870335877,
0.08940795809030533,
0.004703911487013102,
-0.03623355180025101,
0.06710724532604218,
-0.017088884487748146,
0.042834434658288956,
-0.03142407536506653,
0.012517153285443783,
0.03866264969110489,
-0.029783999547362328,
-0.028415480628609657,
0.04544723778963089,
-0.03648237884044647,
0.003853407222777605,
-0.04362262785434723,
0.05015837773680687,
-0.04560929909348488,
0.022010529413819313,
0.05461083725094795,
0.0005312394932843745,
0.056352801620960236,
-0.0417022630572319,
0.014105187729001045,
-0.28274983167648315,
-0.012523530051112175,
0.07107719779014587,
0.019071390852332115,
0.05770309269428253,
-0.028076129034161568,
0.0675046294927597,
-0.027634931728243828,
0.016194147989153862,
-0.052847277373075485,
0.0075136516243219376,
0.04682311788201332,
-0.006928338203579187,
-0.004421493969857693,
-0.005479254759848118,
0.03539823740720749,
0.03701955825090408,
0.010575050488114357,
0.010500943288207054,
-0.006400223821401596,
0.004682552069425583,
-0.02127501368522644,
-0.04158497974276543,
0.06680255383253098,
0.03959696739912033,
0.002741764299571514,
0.09479574859142303,
0.0014427416026592255,
-0.014008542522788048,
-0.02708013541996479,
0.03457656502723694,
0.016732247546315193,
-0.0430741161108017,
-0.08619079738855362,
-0.03230259194970131,
-0.06439006328582764,
0.05874589830636978,
-0.02960372157394886,
0.1057402715086937,
-0.010857470333576202,
-0.06796260923147202,
0.09102632105350494,
-0.0029864853713661432,
-0.057577695697546005,
-0.02486046589910984,
0.01554308645427227,
-0.03807186335325241,
0.035092346370220184,
0.000184973978321068,
-0.016071343794465065,
0.05419526994228363,
-0.011310800909996033,
0.02489059418439865,
-0.05339900404214859,
0.027531737461686134,
0.015988081693649292,
-0.025182120501995087,
0.04040350392460823,
-0.024554213508963585,
-0.041277460753917694,
-0.025116203352808952,
0.027663039043545723,
-0.01848338171839714,
0.0014028671430423856,
-0.010344894602894783,
0.03179239481687546,
0.013744263909757137,
-0.007417749613523483,
0.043058041483163834,
-0.019595513120293617,
0.03840025141835213,
0.10064352303743362,
-0.026084857061505318,
0.018493039533495903,
0.11254797875881195,
-0.013796597719192505,
-0.03344205021858215,
-0.020099986344575882,
-0.010286503471434116,
0.03784947842359543,
-0.03315427526831627,
-0.014528174884617329,
0.02232510782778263,
0.009581624530255795,
-0.03956833481788635,
-0.0016893481370061636,
-0.019226785749197006,
-0.0033851435873657465,
0.03369450569152832,
-0.030764581635594368,
-0.016096293926239014,
0.0379800945520401,
0.0013835892314091325,
0.006570836994796991,
0.03912554681301117,
0.020430386066436768,
-0.273069828748703,
-0.0002289979311171919,
0.06239364296197891,
0.00820179469883442,
-0.032654568552970886,
-0.01502761896699667,
0.032723624259233475,
-0.020597482100129128,
-0.04164598137140274,
-0.06036420539021492,
-0.014955487102270126,
-0.001079219626262784,
0.024949338287115097,
0.03019852004945278,
-0.02482559159398079,
0.011828889138996601,
0.06102633476257324,
-0.03922995179891586,
-0.03704848885536194,
-0.010670077055692673,
-0.024991195648908615,
0.022137228399515152,
0.11867360025644302,
0.009584859944880009,
-0.04079705476760864,
0.05530733987689018,
0.0015900545986369252,
0.0679883137345314,
-0.008578368462622166,
0.005535713396966457,
-0.02205057628452778,
0.04289960116147995,
0.048978403210639954,
-0.0364861786365509,
0.04376175254583359,
-0.02941945381462574,
-0.024396387860178947,
0.038724031299352646,
-0.02454349771142006,
-0.02018248662352562,
0.002002064138650894,
0.016633840277791023,
-0.04914316534996033,
0.04208787530660629,
0.01727704145014286,
-0.04437893256545067,
-0.0779997855424881,
0.004346679896116257,
-0.01925133541226387,
0.02881144918501377,
-0.031833402812480927,
-0.014715699478983879,
-0.0018602453637868166,
0.03609873354434967,
0.03533192351460457,
0.027408014982938766,
-0.04808850586414337,
-0.03590220957994461,
0.027475442737340927,
-0.01115773618221283,
-0.014736957848072052,
0.009168548509478569,
-0.00011013491166522726,
0.06923505663871765,
0.05520325154066086
] |
PMID:18599
|
Differences in pulmonary and cardiovascular beta receptors in the guinea pig and rabbit.
|
An in vivo preparation, in which a body plethysmograph was incorporated, was useful in monitoring the cardiopulmonary effects of pharmacological agents in the guinea pig and rabbit. Isoproterenol, given 30 seconds prior to histamine challenge, reproducibly blocked histamine-induced dynamic compliance decreases and increased heart in the artificially ventilated guinea pig. These effects were used to separate the activity of beta adrenergic blockers on airway and heart muscle. Dose-response data were obtained and ED50 values for pulmonary and cardiovascular blockade were compared. Relative potencies and cardioselectivity ratios for dichloroisoproterenol, practolol, dl-propranolol and d-propranolol were determined. Both practolol and dichloroisoproterenol were cardioselective; dl-propranolol was found to be the most potent. When a similar protocol was tried in the rabbit, isorpoterenol failed to antagonize either histamine or methacholine-induced airway constriction. This finding was supported in subsequent in vitro tests. Isoproterenol and epinephrine were ineffective in blocking methacholine-induced tracheal chain contractions and epinephrine did not significantly enhance adenylate cyclase activity. Our observations suggest rabbit airway smooth muscle is insensitive to beta adrenergic stimulants.
|
Differences in pulmonary and cardiovascular beta receptors in the guinea pig and rabbit. An in vivo preparation, in which a body plethysmograph was incorporated, was useful in monitoring the cardiopulmonary effects of pharmacological agents in the guinea pig and rabbit. Isoproterenol, given 30 seconds prior to histamine challenge, reproducibly blocked histamine-induced dynamic compliance decreases and increased heart in the artificially ventilated guinea pig. These effects were used to separate the activity of beta adrenergic blockers on airway and heart muscle. Dose-response data were obtained and ED50 values for pulmonary and cardiovascular blockade were compared. Relative potencies and cardioselectivity ratios for dichloroisoproterenol, practolol, dl-propranolol and d-propranolol were determined. Both practolol and dichloroisoproterenol were cardioselective; dl-propranolol was found to be the most potent. When a similar protocol was tried in the rabbit, isorpoterenol failed to antagonize either histamine or methacholine-induced airway constriction. This finding was supported in subsequent in vitro tests. Isoproterenol and epinephrine were ineffective in blocking methacholine-induced tracheal chain contractions and epinephrine did not significantly enhance adenylate cyclase activity. Our observations suggest rabbit airway smooth muscle is insensitive to beta adrenergic stimulants.
|
[
-0.06119523569941521,
-0.02953505329787731,
-0.006841600406914949,
0.0442088283598423,
0.026975905522704124,
0.009910687804222107,
0.010461045429110527,
0.020719124004244804,
-0.05134554207324982,
-0.02810896746814251,
-0.0015109586529433727,
-0.036044102162122726,
-0.03109608218073845,
0.035345859825611115,
-0.027688199654221535,
0.00020567848696373403,
0.02089873142540455,
-0.03773413226008415,
-0.07153230905532837,
-0.01770557463169098,
-0.05901611968874931,
0.00015380563854705542,
0.009591341949999332,
-0.018263207748532295,
0.023866066709160805,
0.024131467565894127,
-0.04752064868807793,
0.023488527163863182,
-0.02864103950560093,
-0.20131483674049377,
-0.0018963251495733857,
0.00159138860180974,
0.045198481529951096,
0.00045011346810497344,
-0.024782542139291763,
0.02029452472925186,
0.018517887219786644,
0.0034934906288981438,
-0.0051590134389698505,
0.04669411480426788,
-0.0188295841217041,
-0.06382844597101212,
0.028368787840008736,
0.0037458317819982767,
0.01211559772491455,
-0.016179123893380165,
-0.030244862660765648,
-0.042590104043483734,
0.09914197027683258,
-0.042323607951402664,
0.04370483383536339,
-0.008866729214787483,
-0.03232667222619057,
0.028028063476085663,
-0.011784789152443409,
-0.033938948065042496,
0.023492461070418358,
0.0610164999961853,
0.01755630038678646,
0.026698382571339607,
-0.013973096385598183,
0.02991613931953907,
-0.15213645994663239,
0.08486654609441757,
0.004769343417137861,
0.049524422734975815,
-0.015051896683871746,
-0.02491854690015316,
0.05187635123729706,
0.060740601271390915,
0.011860307306051254,
0.006646003108471632,
0.024373792111873627,
-0.003357665380463004,
-0.017294783145189285,
0.014190267771482468,
0.007066173013299704,
-0.009377237409353256,
0.028517410159111023,
0.0211806483566761,
-0.022407349199056625,
0.023097356781363487,
-0.05898869410157204,
-0.03437642380595207,
0.023326264694333076,
-0.03253151848912239,
0.07524489611387253,
-0.01898484118282795,
-0.018100660294294357,
-0.03165567293763161,
-0.06675409525632858,
-0.049355436116456985,
0.007883247919380665,
0.012151445262134075,
-0.021360717713832855,
0.019243935123085976,
-0.007360606919974089,
-0.04750145226716995,
-0.03246687725186348,
0.3750440776348114,
0.02141769975423813,
0.020135316997766495,
-0.1025647297501564,
0.017883097752928734,
0.03901589661836624,
-0.01612826995551586,
-0.008422301150858402,
-0.01810908503830433,
-0.034509122371673584,
0.06236035376787186,
0.08079467713832855,
0.0017142315628007054,
0.05588436499238014,
-0.03210216760635376,
-0.04168964549899101,
-0.05627865344285965,
0.04148028790950775,
-0.017438802868127823,
0.020269982516765594,
-0.003434916492551565,
0.004146462306380272,
0.007420816924422979,
0.006438641343265772,
0.01642240583896637,
0.04011368751525879,
-0.005673923529684544,
-0.03687835484743118,
0.08447270840406418,
0.03746498376131058,
0.041286665946245193,
0.047536786645650864,
0.056938670575618744,
0.00748351588845253,
0.023413697257637978,
-0.012815230526030064,
-0.05920156091451645,
0.026674900203943253,
0.0009184991358779371,
0.04514961689710617,
-0.010702908039093018,
-0.026176990941166878,
-0.0530194453895092,
0.0200383048504591,
-0.08124887943267822,
-0.10632272064685822,
0.08893264085054398,
0.032639823853969574,
-0.0012141045881435275,
-0.05222231522202492,
0.020495276898145676,
-0.026140617206692696,
0.022240908816456795,
-0.023791128769516945,
0.0793423056602478,
-0.04844290390610695,
0.01450261753052473,
0.008299624547362328,
0.049237266182899475,
-0.0028238107915967703,
-0.04392922669649124,
-0.10935402661561966,
-0.08717597275972366,
-0.02044571377336979,
0.036404579877853394,
-0.01010208111256361,
-0.001604945515282452,
-0.03861340507864952,
0.018248243257403374,
0.014824834652245045,
0.04135400056838989,
-0.015401546843349934,
-0.005049880128353834,
-0.027123156934976578,
-0.0011568982154130936,
-0.07649791240692139,
0.011508435010910034,
0.045482926070690155,
-0.03248569369316101,
0.06577135622501373,
-0.02308722399175167,
-0.02735939994454384,
0.010058967396616936,
0.04265955835580826,
-0.004285648465156555,
0.02054409496486187,
0.06722483038902283,
-0.05854863300919533,
-0.008507085032761097,
0.03652603551745415,
0.0529199093580246,
-0.00035742082400247455,
-0.032530494034290314,
-0.07048463821411133,
0.012331799603998661,
-0.036242131143808365,
-0.04016947001218796,
-0.041798584163188934,
0.06377694755792618,
-0.02017565257847309,
0.02468695119023323,
0.07450596243143082,
0.0016320307040587068,
0.03598935529589653,
0.02361384406685829,
-0.02569412626326084,
0.042536865919828415,
-0.0022242427803575993,
-0.020585021004080772,
0.06406974047422409,
-0.02501622773706913,
-0.01008157804608345,
0.050707023590803146,
0.05384381487965584,
0.014077243395149708,
-0.07399628311395645,
0.053244978189468384,
-0.023628106340765953,
-0.00656723789870739,
0.04260183498263359,
0.02227911353111267,
0.004304188769310713,
0.02967194654047489,
0.017798569053411484,
-0.3270817995071411,
-0.06320609897375107,
-0.006544921081513166,
-0.011483335867524147,
0.054521363228559494,
-0.047931160777807236,
0.03543071076273918,
-0.0454164519906044,
-0.03212069347500801,
-0.05263449624180794,
0.011090180836617947,
0.049333274364471436,
-0.03792601078748703,
0.0056232912465929985,
0.0019038893515244126,
0.08173559606075287,
0.03522679582238197,
-0.023384658619761467,
-0.012336560525000095,
0.01672522723674774,
0.03228582814335823,
-0.044665392488241196,
-0.04883105307817459,
0.023556282743811607,
0.042423784732818604,
0.030723165720701218,
0.10847073048353195,
-0.004417092073708773,
-0.016871822997927666,
0.03152688220143318,
-0.001393095008097589,
0.001979610649868846,
-0.0030846488662064075,
-0.0836300179362297,
-0.001587218837812543,
-0.03451857715845108,
0.026191046461462975,
-0.0222791600972414,
0.042625315487384796,
-0.010561318136751652,
-0.04396777227520943,
0.02739730477333069,
0.03261391073465347,
-0.03752049058675766,
-0.05423012375831604,
-0.015148190781474113,
0.008061664178967476,
0.05457326024770737,
-0.03061920776963234,
-0.02835521474480629,
0.0013365264749154449,
0.013497095555067062,
-0.0029174676164984703,
-0.04312462732195854,
0.06435839086771011,
0.008884630165994167,
-0.04451724886894226,
-0.028377560898661613,
-0.014864292927086353,
0.02972463145852089,
0.004352245945483446,
0.024226978421211243,
0.0485072061419487,
0.013199393637478352,
-0.03725665435194969,
0.007789162918925285,
0.06927882879972458,
0.002805200405418873,
0.01316622830927372,
0.009021091274917126,
0.019873229786753654,
0.11024089902639389,
-0.06311336904764175,
-0.0008655604906380177,
0.10519836843013763,
0.01420779712498188,
0.017336660996079445,
-0.024296140298247337,
-0.044992007315158844,
0.035841234028339386,
0.030963869765400887,
-0.05236102640628815,
0.013821922242641449,
0.012106532230973244,
-0.027278399094939232,
-0.032401975244283676,
-0.005259126890450716,
0.01200203038752079,
0.06389012932777405,
-0.040752045810222626,
-0.03914375975728035,
0.017048534005880356,
-0.05039796233177185,
-0.06415687501430511,
0.014459865167737007,
0.022006524726748466,
-0.24532738327980042,
0.0008788109989836812,
0.06398773938417435,
-0.006377769634127617,
0.0076256138272583485,
-0.0266868956387043,
0.04485510289669037,
-0.044283900409936905,
-0.03778355196118355,
-0.019367296248674393,
-0.021326616406440735,
0.00147383869625628,
0.06972170621156693,
0.07171133160591125,
-0.050668682903051376,
0.05005248636007309,
0.04680822044610977,
-0.01163736917078495,
0.041287679225206375,
-0.03350396081805229,
-0.01415353175252676,
-0.05282554775476456,
0.14086365699768066,
0.024478476494550705,
-0.04760030284523964,
0.06681124120950699,
0.023678623139858246,
0.02427450567483902,
-0.01560159120708704,
0.06420034915208817,
-0.02707144245505333,
-0.042591456323862076,
0.10802312195301056,
-0.04788931831717491,
-0.009390408173203468,
0.03350142762064934,
-0.0688573494553566,
0.008700097911059856,
0.003209601389244199,
0.0018440596759319305,
-0.032105401158332825,
0.014509713277220726,
-0.04184767231345177,
-0.060476042330265045,
0.07734636217355728,
-0.0862405076622963,
-0.02465401589870453,
-0.013247436843812466,
-0.000036735851608682424,
0.0409369133412838,
-0.03611940145492554,
-0.03303394094109535,
0.043106503784656525,
0.09745720773935318,
0.01752411760389805,
-0.03269580006599426,
-0.00631207088008523,
-0.011396442539989948,
-0.00788774061948061,
-0.0007611416513100266,
-0.00764067005366087,
-0.011448489502072334,
-0.044566117227077484,
0.07888324558734894,
0.11418221890926361
] |
PMID:18601
|
Phosphofructokinase of Leishmania donovani and Leishmania braziliensis and its role in glycolysis.
|
It was shown in an investigation of the phosphofructokinases of Leishmania donovani and Leishmania braziliensis that both enzymes are similar to that of Crithidia fasciculata. Although the enzymes are allosteric with respect to their substrates and require AMP for activation, there is no influence by other heterotropic modifiers. The Mg2+-ATP chelate activates these enzymes in a first order process and they can be inhibited by free ATP. The inhibition is reversed by the activator, AMP, in a competitive manner. The requirement for the nucleotide in L. donovani can be eliminated by decreasing the pH. The data indicate that phosphofructokinase, a pivotal enzyme in glycolysis for most organisms, probably does not play an important role in glycolysis in Leishmania.
|
Phosphofructokinase of Leishmania donovani and Leishmania braziliensis and its role in glycolysis. It was shown in an investigation of the phosphofructokinases of Leishmania donovani and Leishmania braziliensis that both enzymes are similar to that of Crithidia fasciculata. Although the enzymes are allosteric with respect to their substrates and require AMP for activation, there is no influence by other heterotropic modifiers. The Mg2+-ATP chelate activates these enzymes in a first order process and they can be inhibited by free ATP. The inhibition is reversed by the activator, AMP, in a competitive manner. The requirement for the nucleotide in L. donovani can be eliminated by decreasing the pH. The data indicate that phosphofructokinase, a pivotal enzyme in glycolysis for most organisms, probably does not play an important role in glycolysis in Leishmania.
|
[
-0.020652297884225845,
-0.012192650698125362,
-0.011930988170206547,
0.003501943312585354,
0.013667731545865536,
-0.019489044323563576,
0.06444569677114487,
0.047989025712013245,
-0.109705850481987,
-0.05104634165763855,
0.07656596601009369,
-0.04952488839626312,
-0.06534180045127869,
0.05939656123518944,
0.004673936404287815,
-0.004697485826909542,
-0.03139201179146767,
0.054941851645708084,
-0.04449758678674698,
-0.03286615014076233,
0.024581320583820343,
-0.010533342137932777,
-0.0027899981942027807,
0.011531387455761433,
0.01898317225277424,
0.05240371450781822,
-0.0012164772488176823,
-0.004653356969356537,
-0.04715646430850029,
-0.17312124371528625,
0.02319849468767643,
0.014493853785097599,
-0.02137918211519718,
-0.06603068858385086,
-0.025148091837763786,
0.024089626967906952,
0.003241904079914093,
-0.017092352733016014,
-0.08676334470510483,
0.0018937013810500503,
0.036442048847675323,
-0.0006064864574000239,
0.0320030078291893,
0.017782794311642647,
-0.014077846892178059,
-0.08283748477697372,
-0.04661621153354645,
0.010150905698537827,
0.07426834106445312,
0.002316451631486416,
-0.022496476769447327,
0.0015878037083894014,
-0.016016170382499695,
0.03764205798506737,
-0.019734492525458336,
-0.0609431155025959,
0.021866563707590103,
0.026548832654953003,
0.014331212267279625,
0.0016830329550430179,
0.00938275083899498,
0.03536534681916237,
-0.14305251836776733,
0.013094124384224415,
0.052265409380197525,
-0.06087882071733475,
0.0020309945102781057,
-0.06973358243703842,
-0.009759156964719296,
0.06980000436306,
-0.058405954390764236,
0.0036924872547388077,
0.0493597574532032,
-0.03778864070773125,
0.024774111807346344,
0.02315092831850052,
-0.00021809813915751874,
0.002123724902048707,
0.027239728718996048,
0.045417070388793945,
0.005543023347854614,
-0.001823680941015482,
-0.006250350270420313,
-0.02955709397792816,
-0.039701737463474274,
0.01302297879010439,
0.06431718170642853,
-0.06860221177339554,
0.0012536585563793778,
0.008517893962562084,
-0.02037026733160019,
-0.09315121918916702,
0.06603549420833588,
-0.01425208244472742,
-0.0776887908577919,
0.09648862481117249,
0.012752302922308445,
-0.013817070052027702,
0.027617668733000755,
0.3608173429965973,
0.012881548143923283,
-0.022510439157485962,
-0.0068297116085886955,
0.05955260619521141,
0.030811112374067307,
-0.02345268428325653,
-0.019998908042907715,
-0.009913562797009945,
0.031918108463287354,
0.05472760647535324,
0.08966851234436035,
-0.003550569759681821,
-0.026355842128396034,
-0.028002416715025902,
-0.043904293328523636,
-0.005660406779497862,
0.03360889479517937,
0.03534654900431633,
-0.06601539999246597,
0.01674153469502926,
-0.0060014501214027405,
-0.010103389620780945,
0.038188111037015915,
-0.02628273144364357,
0.04726266860961914,
0.04547560214996338,
-0.01737777329981327,
0.09027545154094696,
0.11406584829092026,
-0.03199789300560951,
0.09894399344921112,
0.038122110068798065,
-0.07173801958560944,
-0.03691868856549263,
0.0008023816044442356,
0.012467932887375355,
0.05385207012295723,
-0.025706803426146507,
0.052412111312150955,
0.018206056207418442,
-0.034744784235954285,
-0.03103725053369999,
-0.024985337629914284,
-0.041611023247241974,
0.05118291825056076,
0.04757710173726082,
-0.047010377049446106,
0.04775377735495567,
-0.04242353141307831,
0.05289604887366295,
0.0026181533467024565,
0.07325171679258347,
-0.0212151687592268,
-0.011119188740849495,
-0.007486290764063597,
0.037197183817625046,
-0.0022164047695696354,
-0.03055594675242901,
0.04755712300539017,
-0.07169459760189056,
-0.12760894000530243,
-0.02864154614508152,
0.027629373595118523,
0.03591423109173775,
-0.002818107372149825,
0.019608918577432632,
0.018967142328619957,
-0.03302692621946335,
-0.05013673007488251,
-0.0016367112984880805,
0.016091622412204742,
-0.04050368443131447,
-0.06393138319253922,
-0.07363075762987137,
-0.036539409309625626,
-0.018076825886964798,
0.024415668100118637,
0.006122521590441465,
0.00415576109662652,
-0.014619092456996441,
0.004851656034588814,
-0.03652719408273697,
0.0241997167468071,
-0.02238219976425171,
0.020034069195389748,
-0.017274415120482445,
-0.011503790505230427,
0.020443446934223175,
-0.037421051412820816,
0.0450485497713089,
-0.028271714225411415,
0.003764728782698512,
-0.03934129700064659,
0.03279316425323486,
-0.0005972880753688514,
-0.04818513244390488,
-0.016115881502628326,
0.013281808234751225,
-0.03967422619462013,
0.012571712024509907,
0.07288578897714615,
0.04222672060132027,
-0.0014783727237954736,
0.03929036110639572,
-0.019155986607074738,
0.08040329068899155,
-0.051336344331502914,
0.031486064195632935,
-0.011131699196994305,
-0.01012702751904726,
-0.009278385899960995,
-0.03724845498800278,
-0.06839068979024887,
-0.002011305885389447,
-0.1116652563214302,
0.06275060772895813,
-0.013173295184969902,
0.0419108048081398,
0.03116524964570999,
-0.002889785682782531,
-0.03049360401928425,
-0.009142281487584114,
-0.00839243270456791,
-0.27151843905448914,
-0.017265725880861282,
-0.05179663375020027,
0.03414240479469299,
0.061825864017009735,
-0.044908806681632996,
0.004285943694412708,
-0.019441036507487297,
0.0047453660517930984,
-0.055449437350034714,
0.05839600786566734,
0.05327117070555687,
0.006494675762951374,
0.006464074365794659,
-0.037288498133420944,
0.058952223509550095,
-0.02276219055056572,
-0.06739810854196548,
0.01948227733373642,
0.0333326980471611,
0.06187954917550087,
-0.007112652063369751,
0.02667345106601715,
-0.008677663281559944,
0.08976223319768906,
-0.01869160495698452,
0.11092865467071533,
0.05374922230839729,
0.0492357537150383,
-0.046291060745716095,
0.04150054231286049,
0.03585607931017876,
-0.02916557714343071,
-0.0902225598692894,
-0.04475722089409828,
-0.04084306210279465,
0.0160855483263731,
-0.08002504706382751,
0.02236483059823513,
-0.049813613295555115,
-0.015868457034230232,
0.04657433182001114,
-0.07569336891174316,
-0.018444444984197617,
-0.006302951835095882,
0.04087641462683678,
-0.015680035576224327,
0.07199724018573761,
0.0002608706708997488,
0.02475910261273384,
0.015021863393485546,
0.07072094827890396,
0.05424985662102699,
0.0045373765751719475,
-0.001991607015952468,
0.04446851834654808,
-0.061583369970321655,
-0.01112984400242567,
-0.08190323412418365,
0.03395127132534981,
-0.011245784349739552,
-0.007924714125692844,
-0.023329051211476326,
0.00801182258874178,
0.008006715215742588,
-0.023480188101530075,
-0.031122205778956413,
0.020773502066731453,
0.05505926162004471,
0.0564090870320797,
0.005734644830226898,
0.07989166676998138,
-0.05262815207242966,
0.028118813410401344,
0.0380064994096756,
-0.017021166160702705,
0.017343223094940186,
0.004608968738466501,
-0.057819075882434845,
0.016640566289424896,
0.02107532136142254,
0.020558370277285576,
0.07827597111463547,
-0.025904003530740738,
-0.038444820791482925,
0.05204210430383682,
0.04851793497800827,
0.022895045578479767,
0.01446913555264473,
-0.04586711898446083,
0.00499682454392314,
-0.01822551153600216,
-0.06711936742067337,
-0.025207867845892906,
0.0057901120744645596,
0.030150549486279488,
-0.24423463642597198,
0.044788941740989685,
0.009739805944263935,
0.043637942522764206,
-0.011058477684855461,
0.025657273828983307,
0.027775445953011513,
-0.04682203754782677,
0.023697230964899063,
0.05861573666334152,
0.04398493468761444,
0.029790354892611504,
0.031996242702007294,
0.06648199260234833,
0.00006186308746691793,
0.02515408582985401,
0.03279910236597061,
-0.05554456263780594,
0.06545848399400711,
-0.036100924015045166,
-0.006853711791336536,
-0.06815248727798462,
0.16765917837619781,
0.002547193318605423,
-0.021517101675271988,
0.05723915249109268,
-0.003248035442084074,
-0.0007028977852314711,
-0.0603577196598053,
-0.025676246732473373,
0.00569485267624259,
-0.03844301030039787,
0.0976051539182663,
0.012060168199241161,
0.025739675387740135,
-0.005709865130484104,
-0.011388162150979042,
0.023503296077251434,
0.0014928759774193168,
-0.02364775724709034,
-0.014773575589060783,
0.007719425018876791,
-0.03385262191295624,
-0.027609290555119514,
0.09821175038814545,
-0.04711225628852844,
-0.01192750595510006,
-0.0672948956489563,
0.01580185256898403,
0.016776978969573975,
-0.03554997965693474,
-0.052039507776498795,
-0.013563788495957851,
-0.018764574080705643,
-0.02216671034693718,
-0.018178429454565048,
-0.04525747522711754,
-0.06950563937425613,
0.02006850391626358,
0.007017561234533787,
0.042728401720523834,
0.09333279728889465,
-0.04079350084066391,
0.061920616775751114,
-0.023039648309350014
] |
PMID:18603
|
Nature of the rabbit acrosome reaction-inducing activity of follicular fluid.
|
Follicular fluid samples from rabbits, cats, pigs, women and cows had acrosome reaction-inducing activity (ARIA) on rabbit spermatozoa as determined by differential staining after incubation with these fluids. Activity was retained after dialysis and at least 50% was found to be labile when heated to 56 degrees C for as little as 20 min. The induction of the acrosome reaction by bovine follicular fluid showed a dependence on the concentration of follicular fluid and spermatozoa and on calcium ions, and had a pH optimum of approximately 8. Enzyme treatments showed that proteases destroyed the ARIA and this activity was completely blocked by treatment of bovine follicular fluid with goat anti-bovine antiserum. Electron microscope observations indicated the similarity of the reactions observed to that occurring in vivo. It is concluded that the rabbit acrosome reaction inducing-activity of bovine follicular fluid is a serum component(s), probably a protein(s) of high molecular weight.
|
Nature of the rabbit acrosome reaction-inducing activity of follicular fluid. Follicular fluid samples from rabbits, cats, pigs, women and cows had acrosome reaction-inducing activity (ARIA) on rabbit spermatozoa as determined by differential staining after incubation with these fluids. Activity was retained after dialysis and at least 50% was found to be labile when heated to 56 degrees C for as little as 20 min. The induction of the acrosome reaction by bovine follicular fluid showed a dependence on the concentration of follicular fluid and spermatozoa and on calcium ions, and had a pH optimum of approximately 8. Enzyme treatments showed that proteases destroyed the ARIA and this activity was completely blocked by treatment of bovine follicular fluid with goat anti-bovine antiserum. Electron microscope observations indicated the similarity of the reactions observed to that occurring in vivo. It is concluded that the rabbit acrosome reaction inducing-activity of bovine follicular fluid is a serum component(s), probably a protein(s) of high molecular weight.
|
[
0.014878420159220695,
0.02014969475567341,
-0.03866817429661751,
0.030443117022514343,
0.002857557963579893,
0.0021271787118166685,
0.02793029509484768,
0.1168912723660469,
-0.024262024089694023,
0.012906903401017189,
-0.00937249418348074,
-0.11149659752845764,
-0.020824894309043884,
0.011390640400350094,
-0.023699406534433365,
-0.007249034475535154,
-0.01444778311997652,
-0.006180986296385527,
-0.07717868685722351,
0.00899129081517458,
-0.002914470387622714,
-0.021006671711802483,
-0.052361566573381424,
-0.016257019713521004,
-0.016879115253686905,
0.06094079092144966,
0.0024721152149140835,
0.037694696336984634,
-0.041798509657382965,
-0.17405006289482117,
0.00671026948839426,
-0.03569793701171875,
-0.006832022685557604,
-0.011552023701369762,
0.04316329210996628,
-0.028203345835208893,
-0.01046055369079113,
-0.03834589943289757,
-0.03972431272268295,
0.02671642228960991,
0.12270428240299225,
-0.0017168084159493446,
-0.022079944610595703,
0.03905496001243591,
0.03583485260605812,
0.020025871694087982,
-0.02376043237745762,
0.027498889714479446,
0.04170244187116623,
-0.010092423297464848,
0.059472423046827316,
-0.01988428831100464,
-0.005249436479061842,
-0.020683888345956802,
-0.012918421998620033,
-0.004430604632943869,
0.039243023842573166,
-0.003296090057119727,
0.006662475876510143,
0.037571463733911514,
-0.005921056494116783,
0.022316666319966316,
-0.10801352560520172,
0.0829363539814949,
0.053115829825401306,
0.017310429364442825,
0.0067484634928405285,
-0.08213099837303162,
0.055279143154621124,
0.06552986800670624,
-0.07513369619846344,
0.054200414568185806,
0.016154294833540916,
0.0013024224899709225,
0.013307248242199421,
0.009043682366609573,
0.016997110098600388,
0.010245886631309986,
0.038454171270132065,
0.030534449964761734,
-0.04291757568717003,
-0.007336118724197149,
0.043315328657627106,
0.02856212481856346,
0.013607335276901722,
-0.025810087099671364,
-0.01187934074550867,
-0.009758667089045048,
0.04190951585769653,
0.04397417604923248,
-0.05801624804735184,
-0.04753106087446213,
-0.00727657787501812,
0.01580231264233589,
-0.02265039086341858,
0.06135307252407074,
0.006921919994056225,
-0.010391215793788433,
0.002744767116382718,
0.3815602660179138,
-0.05301981791853905,
0.021726299077272415,
-0.11889477074146271,
-0.055362459272146225,
-0.03727681189775467,
-0.0007553569157607853,
0.021566445007920265,
-0.00988404918462038,
0.002952829236164689,
0.09622806310653687,
0.045748088508844376,
-0.018793560564517975,
0.03896545618772507,
-0.03889111056923866,
-0.05607421323657036,
-0.022947726771235466,
0.029198279604315758,
0.04935536906123161,
-0.05614857003092766,
0.017554523423314095,
0.014517193660140038,
-0.021480843424797058,
0.02893887273967266,
-0.06040548160672188,
0.0618198998272419,
0.0024981219321489334,
0.07065801322460175,
0.07662162184715271,
-0.011230704374611378,
-0.001579735428094864,
0.02339262329041958,
-0.010104659013450146,
0.03103293851017952,
-0.008488237857818604,
0.05152975767850876,
-0.014283275231719017,
0.023834047839045525,
-0.04822809249162674,
0.008280470035970211,
0.034108035266399384,
0.016093244776129723,
-0.06753051280975342,
-0.04995753616094589,
-0.06794603914022446,
0.015418432652950287,
0.11407826095819473,
0.02837805077433586,
0.03302102908492088,
-0.017947455868124962,
0.022417360916733742,
0.03149574622511864,
0.10333392769098282,
-0.0978337749838829,
0.07851295918226242,
-0.025903278961777687,
-0.017858009785413742,
-0.030655648559331894,
0.061015691608190536,
-0.0053063128143548965,
-0.022875061258673668,
-0.08701171725988388,
-0.06828106939792633,
0.0181574784219265,
0.008873981423676014,
0.021242350339889526,
-0.05781631916761398,
-0.008611478842794895,
0.04195327311754227,
0.0033281040377914906,
-0.02009204402565956,
-0.03409254178404808,
-0.005527057219296694,
-0.06429263204336166,
-0.027817752212285995,
-0.006842257454991341,
0.02548009715974331,
0.0462038479745388,
-0.0002044174907496199,
0.04754169285297394,
0.05601762607693672,
0.031190471723675728,
-0.026916204020380974,
-0.017078977078199387,
-0.009246236644685268,
-0.004885328002274036,
-0.01251793559640646,
-0.09995310008525848,
0.011293511837720871,
0.015930945053696632,
0.02239653654396534,
-0.043453533202409744,
0.0036039447877556086,
-0.03615785017609596,
0.012979034334421158,
-0.029475819319486618,
-0.032712701708078384,
0.04727555066347122,
0.03822317346930504,
0.025555672124028206,
0.0033201812766492367,
0.08122237026691437,
0.0013702024007216096,
0.04447636380791664,
-0.005264055449515581,
0.03153911605477333,
0.0628327801823616,
-0.025571400299668312,
0.023738497868180275,
0.06802424788475037,
-0.013124936260282993,
-0.016645176336169243,
-0.0029269573278725147,
-0.030476592481136322,
-0.03394544869661331,
-0.09436886012554169,
0.09684988856315613,
-0.0441950298845768,
-0.04139332473278046,
0.06125863641500473,
-0.015864068642258644,
0.07272394001483917,
-0.052661724388599396,
-0.04195262864232063,
-0.3349462151527405,
-0.04251161590218544,
0.012535300105810165,
-0.04002228379249573,
0.0615914948284626,
-0.021378757432103157,
0.02857123874127865,
-0.039732206612825394,
0.054212767630815506,
-0.07502543181180954,
0.05493101105093956,
0.05182263255119324,
-0.0160214826464653,
-0.04840545356273651,
0.00010879615001613274,
-0.009320360608398914,
0.023993324488401413,
-0.0625910609960556,
0.056292641907930374,
0.06406024843454361,
-0.07104682922363281,
-0.013145758770406246,
0.03001469559967518,
0.023864921182394028,
0.044121913611888885,
0.03711847960948944,
0.08326632529497147,
0.044676877558231354,
-0.008311419747769833,
-0.04065432772040367,
0.01839691959321499,
0.04841090738773346,
0.012241613119840622,
-0.07670896500349045,
0.016347520053386688,
-0.01529962383210659,
0.02787759155035019,
-0.026050811633467674,
0.003740847809240222,
0.0180299561470747,
-0.03791144862771034,
0.01919383369386196,
-0.03780411556363106,
-0.02927466481924057,
0.002170344116166234,
0.03671865165233612,
0.04017913341522217,
0.003852556459605694,
-0.018476448953151703,
-0.0034676745999604464,
0.04650634154677391,
-0.010492905043065548,
-0.013171211816370487,
-0.0025870695244520903,
-0.059869375079870224,
0.006673028226941824,
-0.049412801861763,
0.01909773424267769,
0.0028963556978851557,
0.0036242289934307337,
0.060728102922439575,
-0.0012559624155983329,
-0.0057892207987606525,
-0.029367074370384216,
0.022680794820189476,
-0.01891348697245121,
-0.05900362879037857,
-0.006491393316537142,
0.025062819942831993,
-0.01369413360953331,
0.009850752539932728,
0.13206486403942108,
-0.03705327957868576,
0.026179835200309753,
0.019820285961031914,
0.0075350659899413586,
-0.00003191687574144453,
-0.013351378031075,
-0.07853052020072937,
0.04619356989860535,
0.06226402893662453,
-0.04836533963680267,
-0.015817176550626755,
-0.028776118531823158,
-0.01583663746714592,
-0.003858000272884965,
-0.03208184987306595,
-0.02561040036380291,
0.011805021204054356,
0.001306729158386588,
0.002339104190468788,
0.025913581252098083,
-0.058763764798641205,
0.0017654652474448085,
0.0020924340933561325,
0.012745287269353867,
-0.2968667447566986,
-0.06898736953735352,
0.010289181023836136,
-0.016707798466086388,
-0.0003818490367848426,
-0.001572058186866343,
0.020517408847808838,
-0.015337438322603703,
-0.02957773581147194,
0.058804530650377274,
-0.015832720324397087,
-0.0016214509960263968,
0.046395063400268555,
0.005469681229442358,
-0.0075735002756118774,
0.020793359726667404,
0.010621746070683002,
-0.011739952489733696,
-0.06581774353981018,
-0.01918637752532959,
-0.008738504722714424,
-0.006897771265357733,
0.13700827956199646,
0.031086396425962448,
0.008813133463263512,
0.0032647328916937113,
0.010236384347081184,
0.018920674920082092,
0.015314565971493721,
-0.036023303866386414,
-0.01682397536933422,
-0.021567240357398987,
-0.008632528595626354,
-0.00868469662964344,
-0.0147640286013484,
-0.003475111909210682,
-0.01094021461904049,
0.05277388542890549,
0.010733545757830143,
-0.03263325244188309,
-0.006183469668030739,
0.007253052666783333,
-0.05656768009066582,
-0.05500093102455139,
0.0008065046276897192,
-0.034662533551454544,
-0.038441020995378494,
-0.03900352865457535,
-0.01873210445046425,
0.0010396164143458009,
-0.03667041286826134,
-0.045615699142217636,
0.0364842414855957,
0.01897948421537876,
0.050159111618995667,
-0.019403109326958656,
0.0071550775319337845,
0.024448314681649208,
-0.02366192638874054,
0.06234904006123543,
0.062333378940820694,
0.0028979445341974497,
-0.020523134618997574,
0.07527454942464828,
0.006123099010437727
] |
PMID:18606
|
Experimental E. coli arthritis in the rabbit. A model of infectious and post-infectious inflammatory synovitis.
|
Rabbit knee joints challenged with E. coli 06 underwent a self-limited infection lasting several weeks followed by a prolonged post-infectious inflammatory arthritis. The E. coli used did not possess collagenolytic activity nor did a variety of common aerobic clinical isolates. Articular cartilage destruction occurred by two basically different mechanisms: u) direct invasion of pannus at the juxtaarticular margins, and 2) fibrillation in cartilage to cartilage contact areas. Weekly measurement of intra-articular pH and temperature were correlated with bacteriologic findings and groww and microscopic pathologic events.
|
Experimental E. coli arthritis in the rabbit. A model of infectious and post-infectious inflammatory synovitis. Rabbit knee joints challenged with E. coli 06 underwent a self-limited infection lasting several weeks followed by a prolonged post-infectious inflammatory arthritis. The E. coli used did not possess collagenolytic activity nor did a variety of common aerobic clinical isolates. Articular cartilage destruction occurred by two basically different mechanisms: u) direct invasion of pannus at the juxtaarticular margins, and 2) fibrillation in cartilage to cartilage contact areas. Weekly measurement of intra-articular pH and temperature were correlated with bacteriologic findings and groww and microscopic pathologic events.
|
[
-0.06025167182087898,
-0.03127651289105415,
0.06020069122314453,
0.028996210545301437,
0.03856252133846283,
-0.003392338752746582,
-0.000424986588768661,
0.11803270876407623,
-0.028779413551092148,
-0.020200656726956367,
-0.020093193277716637,
-0.014956362545490265,
-0.007588693406432867,
-0.023753099143505096,
-0.019154198467731476,
-0.054370928555727005,
-0.018120473250746727,
0.02467522770166397,
-0.10547952353954315,
0.04088431969285011,
-0.010967697016894817,
-0.013134264387190342,
-0.00911720935255289,
-0.04694467410445213,
-0.05008995160460472,
0.04478275775909424,
0.02136351354420185,
0.02546417899429798,
-0.026480156928300858,
-0.16283152997493744,
-0.0024472675286233425,
-0.00692584877833724,
-0.004087796900421381,
-0.002261454937979579,
-0.0005782495136372745,
0.020604779943823814,
-0.036370519548654556,
-0.05061866715550423,
-0.029234860092401505,
-0.007078905589878559,
0.03715740516781807,
0.04569569602608681,
0.026698531582951546,
0.02077104151248932,
0.07310256361961365,
-0.020943524315953255,
-0.05458185821771622,
0.04074220359325409,
0.09001710265874863,
0.006839873269200325,
0.04777226224541664,
-0.025430670008063316,
0.006008456461131573,
0.03471457213163376,
0.0066494205966591835,
0.003154766047373414,
0.03565598651766777,
0.01603003777563572,
-0.006521057803183794,
0.009647246450185776,
0.06257723271846771,
0.0503540076315403,
-0.10767178982496262,
0.09667559713125229,
0.03381965681910515,
-0.009316629730165005,
0.025711823254823685,
-0.030781645327806473,
0.09955845773220062,
0.07708035409450531,
-0.06497514247894287,
-0.002089482033625245,
-0.031618453562259674,
0.04743507504463196,
-0.011837557889521122,
0.010239790193736553,
0.016541339457035065,
0.011205418035387993,
0.06127052381634712,
0.04515278711915016,
0.0014990625204518437,
0.015008368529379368,
0.02377309836447239,
0.018413744866847992,
-0.04631432145833969,
-0.011069281958043575,
0.024822043254971504,
-0.042717255651950836,
0.007363579701632261,
0.04946678504347801,
-0.032128818333148956,
0.007804060820490122,
-0.008273494429886341,
0.02731483429670334,
-0.05760295316576958,
-0.021597320213913918,
0.035914480686187744,
-0.02886672504246235,
-0.019911112263798714,
0.4148832857608795,
-0.09512747079133987,
-0.050349704921245575,
0.020501697435975075,
0.016284970566630363,
0.022096188738942146,
-0.0024944739416241646,
0.020434850826859474,
0.011635114438831806,
-0.011144041083753109,
0.08390230685472488,
0.0458671897649765,
-0.0014401403022930026,
-0.04365038126707077,
-0.02315560169517994,
0.017180616036057472,
-0.021034374833106995,
0.05884155258536339,
0.016958344727754593,
-0.03448488190770149,
0.017064811661839485,
0.004808403085917234,
-0.012537469156086445,
0.02408447489142418,
-0.031962230801582336,
0.00015938706928864121,
0.010948225855827332,
0.031015856191515923,
0.09991930425167084,
0.015604283660650253,
-0.03936333954334259,
0.04215565696358681,
-0.07222159951925278,
-0.028698138892650604,
0.010017234832048416,
-0.007245371118187904,
0.006912777200341225,
0.023973384872078896,
-0.031121117994189262,
0.011384069919586182,
0.031252678483724594,
0.016200266778469086,
-0.08484204113483429,
-0.022984003648161888,
-0.09186483919620514,
-0.06960735470056534,
0.11472871899604797,
-0.01848836988210678,
0.00009206614777212963,
-0.04253803938627243,
0.028195278719067574,
0.03518792614340782,
0.054700564593076706,
0.0020501601975411177,
0.06811793893575668,
0.0069205546751618385,
0.0005032899789512157,
0.04225454851984978,
0.003609481966122985,
-0.012160655111074448,
-0.027971137315034866,
-0.08597917854785919,
-0.018351981416344643,
0.014303101226687431,
0.04777168855071068,
0.03780955448746681,
-0.008607745170593262,
0.013509809039533138,
0.0005445831920951605,
0.03586531803011894,
0.038219377398490906,
0.02368360571563244,
0.0014972381759434938,
-0.0185075793415308,
-0.041373223066329956,
-0.04402324929833412,
0.08540232479572296,
0.012087187729775906,
0.040520790964365005,
0.011089267209172249,
0.0743771493434906,
-0.028746766969561577,
-0.013689417392015457,
0.016169723123311996,
0.06598923355340958,
0.026227064430713654,
0.03681345283985138,
-0.05439174547791481,
0.046597566455602646,
0.07342275977134705,
0.025956012308597565,
-0.006082550156861544,
-0.06863754242658615,
-0.06360470503568649,
-0.020035244524478912,
0.020103085786104202,
0.010358057916164398,
-0.009029646404087543,
0.04504545032978058,
0.01012770552188158,
0.032249558717012405,
0.047106921672821045,
0.0010187348816543818,
-0.033732082694768906,
0.022136380895972252,
0.0015333151677623391,
0.03020142950117588,
0.027428332716226578,
0.011228150688111782,
-0.04333413392305374,
-0.06982347369194031,
-0.07487655431032181,
0.0033532544039189816,
-0.027963662520051003,
-0.004639329854398966,
-0.04556459188461304,
0.025820907205343246,
-0.01922611892223358,
-0.05584430694580078,
0.037396568804979324,
0.004645762499421835,
-0.019273482263088226,
-0.009876965545117855,
0.0019445625366643071,
-0.31715306639671326,
0.01323887798935175,
0.016048507764935493,
0.0035848391707986593,
0.025910044088959694,
-0.07439951598644257,
-0.027625512331724167,
-0.08187413960695267,
0.0065069496631622314,
-0.03128248080611229,
0.03789200633764267,
0.09084605425596237,
-0.04507618397474289,
-0.07405275851488113,
-0.001042111311107874,
0.042863622307777405,
0.02038058079779148,
-0.003938744310289621,
-0.0509820431470871,
0.027125289663672447,
0.019675306975841522,
0.008832856081426144,
0.060101136565208435,
0.01434518676251173,
0.09023940563201904,
0.002968586515635252,
0.13365647196769714,
0.029190940782427788,
0.0038394543807953596,
-0.0486878827214241,
0.013474159874022007,
0.05201832205057144,
-0.07928469032049179,
-0.055458154529333115,
-0.06060793995857239,
-0.03254522755742073,
-0.013521980494260788,
-0.006906840484589338,
0.000006905816462676739,
-0.010491866618394852,
-0.0520731583237648,
0.014715828001499176,
0.022775933146476746,
-0.08533629775047302,
-0.00025988579727709293,
-0.03258667513728142,
0.028365125879645348,
-0.01662117801606655,
0.008017062209546566,
0.04572714865207672,
0.0031142584048211575,
-0.01393980160355568,
-0.004609157331287861,
0.005926775746047497,
-0.052115973085165024,
0.032427433878183365,
-0.00004495742541621439,
-0.013997559435665607,
-0.04702459275722504,
-0.03501474857330322,
-0.025150610134005547,
-0.025474540889263153,
0.06468319892883301,
-0.012262003496289253,
0.04166564345359802,
-0.06627347320318222,
0.0172914806753397,
-0.005680798552930355,
0.0024523313622921705,
0.00933932326734066,
-0.0010223246645182371,
0.118560291826725,
0.0019816679414361715,
0.024723317474126816,
-0.006334150675684214,
0.019723374396562576,
-0.0009146904922090471,
-0.03782539814710617,
-0.08450688421726227,
0.032715026289224625,
-0.007257194723933935,
-0.10610027611255646,
0.03339967131614685,
-0.04363942891359329,
0.000812147103715688,
-0.00406659347936511,
-0.06972481310367584,
-0.03756381943821907,
0.02217899076640606,
0.009081082418560982,
0.03802061453461647,
-0.0192440003156662,
-0.057847682386636734,
0.004129623528569937,
-0.003582398872822523,
-0.007256121374666691,
-0.25504815578460693,
0.010778406634926796,
0.04999663680791855,
-0.009356401860713959,
-0.012402419932186604,
0.04200716316699982,
-0.01003237534314394,
-0.009123691357672215,
0.00012605862866621464,
0.03673973307013512,
0.027766844257712364,
-0.005934700835496187,
0.040616102516651154,
-0.0026314302813261747,
-0.01264046598225832,
-0.004373014438897371,
0.027476148679852486,
-0.01940390095114708,
-0.020389392971992493,
0.014989353716373444,
-0.03211337327957153,
-0.09631671011447906,
0.09235776215791702,
0.026447081938385963,
0.017846180126070976,
0.044789236038923264,
0.0113641656935215,
0.016750002279877663,
-0.09063120931386948,
-0.018872912973165512,
0.0157026294618845,
-0.021298158913850784,
-0.034386955201625824,
-0.004345707129687071,
0.0062702130526304245,
0.004758500959724188,
-0.02593432366847992,
0.072434201836586,
-0.0009313211194239557,
0.009694399312138557,
-0.037249382585287094,
0.014559837989509106,
0.005819027777761221,
-0.049630697816610336,
0.061357468366622925,
0.00919270422309637,
0.0056973774917423725,
-0.0812312662601471,
0.02512984164059162,
0.014250592328608036,
-0.04524442180991173,
-0.045366816222667694,
0.034455306828022,
0.04213415086269379,
0.033209677785634995,
-0.027449503540992737,
0.018734144046902657,
-0.014101898297667503,
0.00911922287195921,
-0.05534443259239197,
0.06586962193250656,
-0.03131530433893204,
-0.09963652491569519,
0.07919640839099884,
-0.0054197367280721664
] |
PMID:18608
|
An analysis of the professional performance of physician's assistants.
|
The job performance of a national sample of 939 physician's assistants has been assessed by means of a self-rating scale as well as by a rating scale completed by supervising physicians. Three-quarters of the participating supervising physicians are greatly satisfied with the work of their physician's assistants and a similar proportion indicate that they would definitely hire the same assistant if they "had it to do over again." The influences of background and work environment characteristics upon performance were assessed by means of multiple regression. Graduating from a military-sponsored physician's assistant program appears to have a beneficial effect upon performance. Performance during training exerts only a weak effect upon job performance. Amount of education and medical experience prior to beginning physician's assistant training do not affect job performance.
|
An analysis of the professional performance of physician's assistants. The job performance of a national sample of 939 physician's assistants has been assessed by means of a self-rating scale as well as by a rating scale completed by supervising physicians. Three-quarters of the participating supervising physicians are greatly satisfied with the work of their physician's assistants and a similar proportion indicate that they would definitely hire the same assistant if they "had it to do over again." The influences of background and work environment characteristics upon performance were assessed by means of multiple regression. Graduating from a military-sponsored physician's assistant program appears to have a beneficial effect upon performance. Performance during training exerts only a weak effect upon job performance. Amount of education and medical experience prior to beginning physician's assistant training do not affect job performance.
|
[
-0.032570939511060715,
0.05305963009595871,
0.01300016324967146,
-0.016552751883864403,
0.008235996589064598,
-0.014326957054436207,
0.05458514019846916,
0.03394991531968117,
-0.023240992799401283,
-0.0012027572374790907,
0.055827971547842026,
0.06021146476268768,
-0.003997266758233309,
-0.03996145725250244,
-0.06988395750522614,
-0.028219494968652725,
-0.026794252917170525,
0.025774197652935982,
-0.0004964218242093921,
0.023906579241156578,
-0.0364220030605793,
0.02305406890809536,
0.009427876211702824,
0.021428115665912628,
0.01612565666437149,
0.011474927887320518,
-0.06451927125453949,
-0.02812984026968479,
-0.019611595198512077,
-0.09874127805233002,
-0.04060366377234459,
-0.03123020939528942,
0.022503361105918884,
0.053590644150972366,
-0.011805725283920765,
0.0520528182387352,
-0.036306288093328476,
0.09302454441785812,
0.01025110762566328,
0.016245953738689423,
0.021833989769220352,
-0.032509345561265945,
-0.07004106044769287,
-0.009351830929517746,
0.008521727286279202,
-0.03072666935622692,
-0.013943694531917572,
-0.10875323414802551,
0.003793375799432397,
0.055350180715322495,
0.017963211983442307,
-0.04498767852783203,
0.011924002319574356,
0.06857436895370483,
0.04402286931872368,
-0.021723126992583275,
0.02291802130639553,
0.05962684005498886,
-0.03953567147254944,
-0.014945014379918575,
-0.0017706684302538633,
0.06441906839609146,
-0.18205130100250244,
0.0642436072230339,
0.024953259155154228,
0.07043114304542542,
-0.08071798831224442,
-0.028010986745357513,
-0.058673154562711716,
-0.03271636739373207,
-0.03219595178961754,
0.037649836391210556,
-0.024057092145085335,
0.016169818118214607,
0.003949258476495743,
0.07106746733188629,
-0.024492038413882256,
-0.029323486611247063,
0.04299427568912506,
0.03436233103275299,
0.0144403837621212,
0.015224570408463478,
-0.013650295324623585,
-0.02772769145667553,
0.040820032358169556,
-0.0004406030348036438,
0.026645272970199585,
-0.01474340632557869,
0.018247351050376892,
-0.04230036959052086,
0.03946734219789505,
0.012328371405601501,
-0.04475220665335655,
-0.04268663004040718,
0.016310522332787514,
-0.04518641531467438,
0.01798347383737564,
-0.01085848081856966,
-0.05816265940666199,
0.432279497385025,
-0.0377129465341568,
0.008260012604296207,
0.010921573266386986,
-0.012019774876534939,
0.04560180380940437,
-0.021268634125590324,
0.02801586501300335,
-0.0219869427382946,
-0.019011585041880608,
-0.004407597240060568,
0.05356205254793167,
0.008013488724827766,
0.00489125307649374,
-0.02358977310359478,
-0.013107224367558956,
-0.0007343919714912772,
0.04941834881901741,
0.09378115832805634,
0.052924320101737976,
-0.012925282120704651,
0.04681195691227913,
0.03690198436379433,
0.021538885310292244,
-0.018580352887511253,
-0.019617505371570587,
-0.08825212717056274,
0.06161297857761383,
0.08644699305295944,
0.00020075506472494453,
-0.013857629150152206,
0.02638915181159973,
-0.06177068501710892,
-0.06769615411758423,
-0.00883444957435131,
0.02963745780289173,
-0.012490523047745228,
0.008433373644948006,
0.007015525829046965,
-0.05557936802506447,
0.038625605404376984,
0.007800026331096888,
-0.03309556096792221,
-0.031442899256944656,
-0.03918337821960449,
-0.07804261147975922,
0.15080437064170837,
0.029160819947719574,
0.04567110911011696,
0.0020011865999549627,
-0.0002532940707169473,
-0.024547578766942024,
0.08551469445228577,
-0.10337943583726883,
0.0038383142091333866,
-0.037002723664045334,
0.057426340878009796,
0.01423854473978281,
-0.011602611280977726,
0.04465056210756302,
-0.027120722457766533,
-0.011770987883210182,
-0.05911369249224663,
-0.0017021427629515529,
0.04301225021481514,
0.038582898676395416,
-0.00762908672913909,
0.012400110252201557,
-0.042127203196287155,
-0.00047945024562068284,
-0.004328771494328976,
0.0322580561041832,
-0.07074996829032898,
-0.02209886536002159,
-0.008431014604866505,
-0.02834722399711609,
-0.058548133820295334,
-0.012302065268158913,
0.02546844631433487,
-0.009216807782649994,
-0.011676808819174767,
0.07129620015621185,
0.023504748940467834,
-0.024883953854441643,
0.008052011951804161,
-0.003729000687599182,
0.037388142198324203,
-0.014800027944147587,
0.030836893245577812,
0.030876267701387405,
0.017054365947842598,
-0.03860340639948845,
-0.014788573607802391,
-0.051143839955329895,
-0.0061696795746684074,
0.023804686963558197,
-0.005396966356784105,
-0.018599873408675194,
-0.010532031767070293,
-0.03542084991931915,
-0.018286483362317085,
-0.027910463511943817,
0.050391409546136856,
0.026053791865706444,
0.07144426554441452,
-0.014192122966051102,
0.018725909292697906,
-0.005504582542926073,
-0.00788778718560934,
0.032225102186203,
0.03716881200671196,
-0.0528809018433094,
0.003694320796057582,
0.03146069124341011,
-0.010762088932096958,
0.023381300270557404,
-0.018838007003068924,
0.04556547850370407,
0.11064580827951431,
0.0398145467042923,
0.026773318648338318,
0.06862840056419373,
0.08495046943426132,
0.06831926852464676,
-0.3101428747177124,
0.01519913598895073,
-0.023981153964996338,
-0.005666295066475868,
-0.01747172139585018,
-0.025507036596536636,
0.027645794674754143,
0.04112307354807854,
-0.026952024549245834,
-0.006915984209626913,
0.010272513143718243,
-0.02528047189116478,
-0.014611180871725082,
0.02563677728176117,
-0.017768247053027153,
-0.006044791545718908,
-0.017751064151525497,
0.015885822474956512,
-0.030871843919157982,
-0.02822817489504814,
0.06393803656101227,
0.057926807552576065,
0.026032233610749245,
0.006032734178006649,
0.03305758163332939,
0.024722347036004066,
0.07057471573352814,
-0.0920611172914505,
-0.028063327074050903,
0.024694202467799187,
-0.0011859278893098235,
0.03089032508432865,
-0.06971154361963272,
-0.08176818490028381,
0.048101190477609634,
-0.021464979276061058,
0.02406063675880432,
-0.01872694492340088,
-0.01591803878545761,
-0.013473453931510448,
-0.0510309636592865,
0.04471096396446228,
-0.029968539252877235,
0.03881208971142769,
-0.0895347148180008,
-0.03103558160364628,
-0.04239020124077797,
0.014502430334687233,
-0.07588880509138107,
0.033000390976667404,
-0.022608531638979912,
-0.06510657072067261,
-0.01030039694160223,
0.015620158053934574,
-0.01728905737400055,
-0.043207138776779175,
-0.07543477416038513,
0.05350012704730034,
-0.06955430656671524,
0.08391403406858444,
-0.05822419747710228,
-0.011787586845457554,
0.009661868214607239,
0.0156836099922657,
0.03021453320980072,
-0.006940687540918589,
0.006550893187522888,
0.027438925579190254,
0.017848679795861244,
-0.00003996866871602833,
-0.007526060100644827,
0.04698038101196289,
-0.053319595754146576,
-0.012862980365753174,
0.032708052545785904,
-0.02012135088443756,
-0.048698388040065765,
-0.03168633580207825,
-0.01872078888118267,
-0.0187455415725708,
0.016020972281694412,
-0.003400917397812009,
-0.0008254371932707727,
0.06311626732349396,
0.014794377610087395,
-0.008510356768965721,
-0.009646070189774036,
0.06441538780927658,
0.009196119382977486,
-0.0158406849950552,
-0.04192107170820236,
0.06391418725252151,
-0.03464610502123833,
0.03182143345475197,
-0.02896835468709469,
0.04175451397895813,
-0.27397623658180237,
0.002795707667246461,
-0.05048777535557747,
0.026952756568789482,
0.013690846972167492,
0.015342985279858112,
-0.04753870889544487,
-0.04020573943853378,
-0.016552507877349854,
0.008217539638280869,
0.06065456569194794,
0.00651881517842412,
-0.04129709675908089,
0.0011145060416311026,
-0.009615784510970116,
0.03544100746512413,
0.036937106400728226,
-0.02831006981432438,
0.03503319248557091,
-0.04899081960320473,
0.020721467211842537,
0.0027247928082942963,
0.08431123197078705,
-0.05121073126792908,
-0.0014640839071944356,
-0.06190427765250206,
-0.025187082588672638,
-0.0610445961356163,
0.0024499257560819387,
0.021670300513505936,
0.014048412442207336,
-0.019872738048434258,
0.06169216334819794,
0.010343639180064201,
0.014125870540738106,
0.01825380139052868,
-0.007617712486535311,
0.028616823256015778,
-0.03822183981537819,
0.004906033165752888,
0.036175645887851715,
-0.03376288339495659,
0.04104786738753319,
-0.02485845796763897,
0.10823298990726471,
0.041225966066122055,
0.02428639493882656,
-0.09197384119033813,
-0.00026910408632829785,
-0.012860285118222237,
0.0029484734404832125,
0.014418484643101692,
0.06017443910241127,
-0.014369173906743526,
-0.05833722651004791,
0.021959250792860985,
-0.0053642005659639835,
0.01047084853053093,
-0.01885337010025978,
-0.07897167652845383,
0.02808290161192417,
0.018224112689495087,
-0.010320567525923252,
0.09613625705242157,
0.0171767957508564
] |
PMID:18614
|
Glycosidase susceptibility: a probe for the distribution of glycoprotein oligosaccharides in Sindbis virus.
|
Intact Sindbis virus and Triton-solubilized viral glycoprotein were treated with alpha-mannosidase and with a preparation of mixed glycosidases from Diplococcus pneumoniae to probe the accesibility of carbohydrate units on the viral surface. The products of glycosidase attack on Triton-solubilized virus showed that mose carbohydrate units of the glycoproteins are good substrates for these enzymes. The relative resistance of most of the viral oligosaccharides in intact virus particles showed that much of the carbohydrate is not accessible to glycosidases, probably because it is not exposed at the viral surface. The only completely accessible carbohydrate units on Sindbis glycoproteins were the type A oligosaccharides of E2. This differential accessibility of Sindbis oligosaccharides is discussed in relation to the organization of the viral surface.
|
Glycosidase susceptibility: a probe for the distribution of glycoprotein oligosaccharides in Sindbis virus. Intact Sindbis virus and Triton-solubilized viral glycoprotein were treated with alpha-mannosidase and with a preparation of mixed glycosidases from Diplococcus pneumoniae to probe the accesibility of carbohydrate units on the viral surface. The products of glycosidase attack on Triton-solubilized virus showed that mose carbohydrate units of the glycoproteins are good substrates for these enzymes. The relative resistance of most of the viral oligosaccharides in intact virus particles showed that much of the carbohydrate is not accessible to glycosidases, probably because it is not exposed at the viral surface. The only completely accessible carbohydrate units on Sindbis glycoproteins were the type A oligosaccharides of E2. This differential accessibility of Sindbis oligosaccharides is discussed in relation to the organization of the viral surface.
|
[
-0.022152621299028397,
-0.05514342337846756,
-0.057100120931863785,
0.012485401704907417,
0.06784973293542862,
-0.030114764347672462,
0.06155632063746452,
0.03627418726682663,
-0.0941435843706131,
-0.02856430970132351,
0.004342802334576845,
-0.07674352824687958,
-0.02230830304324627,
-0.005691280122846365,
0.025382956489920616,
-0.024318823590874672,
-0.017067795619368553,
-0.03434741869568825,
-0.062076009809970856,
0.0015271817101165652,
0.02218981832265854,
-0.07799360901117325,
0.045850757509469986,
-0.009369414299726486,
0.01832796074450016,
0.050504107028245926,
-0.0009023077436722815,
-0.04290831461548805,
-0.0374179482460022,
-0.2092604786157608,
-0.0090304184705019,
-0.033295124769210815,
0.021874895319342613,
-0.052806954830884933,
-0.027052123099565506,
0.04698427394032478,
-0.004887003917247057,
-0.033870842307806015,
-0.013459065929055214,
0.03488513454794884,
0.008852222003042698,
0.019297441467642784,
-0.031272079795598984,
-0.001341024530120194,
-0.003348825266584754,
-0.044104672968387604,
-0.058247700333595276,
0.04650688171386719,
0.0711560845375061,
0.019062019884586334,
-0.029172128066420555,
0.005378842819482088,
-0.01148581225425005,
-0.008105700835585594,
-0.0006324450368992984,
-0.009483630768954754,
0.014395180158317089,
-0.026210345327854156,
-0.01817573606967926,
0.06822075694799423,
0.015126743353903294,
0.0035435573663562536,
-0.12373274564743042,
0.030733712017536163,
0.05934275686740875,
-0.0019811035599559546,
0.04541994631290436,
-0.015039008110761642,
0.05951561778783798,
0.07996081560850143,
-0.0757407695055008,
0.0021864904556423426,
-0.03163282573223114,
0.02780013717710972,
0.010154150426387787,
0.01969284564256668,
-0.006102182436734438,
0.025805851444602013,
0.03077976405620575,
0.05829392001032829,
-0.015222306363284588,
-0.0028081845957785845,
0.08130163699388504,
-0.06899372488260269,
-0.07388059794902802,
0.05081567540764809,
0.0310838520526886,
0.018814509734511375,
0.03580022230744362,
0.015755051746964455,
-0.01170271635055542,
-0.014016006141901016,
0.001538521726615727,
0.033441610634326935,
-0.10535919666290283,
0.05022352933883667,
0.03366894647479057,
0.011368487030267715,
0.04854879900813103,
0.38226836919784546,
-0.0246895719319582,
-0.005063296295702457,
-0.03151790425181389,
0.014050423167645931,
0.03334930166602135,
-0.04874902591109276,
-0.002772556385025382,
-0.019786765798926353,
0.021745676174759865,
0.05801790952682495,
0.059627141803503036,
0.002369817579165101,
-0.0387495718896389,
-0.03658656030893326,
0.0030776222702115774,
0.0012277794303372502,
-0.025423243641853333,
-0.023181501775979996,
-0.07321087270975113,
0.053612321615219116,
-0.05870398133993149,
-0.05343670770525932,
0.03598763793706894,
-0.033244747668504715,
0.05876542255282402,
0.04295916482806206,
-0.014764607883989811,
0.06703021377325058,
0.0783623605966568,
-0.04748227819800377,
0.06529195606708527,
0.0517197884619236,
0.019684119150042534,
-0.013591852970421314,
-0.002842598594725132,
-0.009792589582502842,
0.013001332059502602,
-0.02076149545609951,
-0.003683720249682665,
0.0032713806722313166,
-0.014824740588665009,
-0.028447167947888374,
-0.003434378420934081,
-0.05486863851547241,
-0.01057184673845768,
-0.0014373870799317956,
-0.05312130227684975,
0.054411880671978,
0.02567995712161064,
-0.024397652596235275,
0.05662670359015465,
0.06359382718801498,
-0.02133995108306408,
-0.009736294858157635,
-0.030878378078341484,
0.0026963588315993547,
0.010320874862372875,
-0.05174596980214119,
0.0065314085222780704,
-0.0022461190819740295,
-0.036506351083517075,
-0.014825016260147095,
-0.0029930616728961468,
0.07843348383903503,
0.01695752702653408,
-0.04215008765459061,
-0.006627918221056461,
0.049805112183094025,
0.05829674378037453,
-0.002991175977513194,
0.007101224269717932,
0.015141553245484829,
-0.03658182919025421,
-0.02218223363161087,
-0.007362603209912777,
0.011282537132501602,
-0.07052619755268097,
0.010997713543474674,
0.011974064633250237,
0.008517911657691002,
-0.008462924510240555,
0.02470027469098568,
-0.01440571341663599,
-0.018736016005277634,
0.03179264813661575,
-0.0271422378718853,
-0.006837442517280579,
0.016438357532024384,
0.023499632254242897,
0.0336090587079525,
-0.018505744636058807,
-0.022794406861066818,
-0.12078110873699188,
-0.012723689898848534,
0.0009367153979837894,
-0.05083740875124931,
0.029276903718709946,
-0.027047697454690933,
0.05105551332235336,
-0.020964739844202995,
0.08566278964281082,
-0.02067272737622261,
0.017730597406625748,
0.053384605795145035,
0.02716943807899952,
-0.038859207183122635,
0.04425830766558647,
-0.026658350601792336,
0.02600770816206932,
0.014389499090611935,
-0.015029159374535084,
-0.04284094274044037,
-0.06679600477218628,
0.011115973815321922,
-0.054829198867082596,
0.07476075738668442,
-0.02024916745722294,
0.050128936767578125,
0.0429961159825325,
0.04091924428939819,
0.03482057526707649,
-0.04550668224692345,
-0.004531782120466232,
-0.2920365333557129,
-0.03706151992082596,
0.01264859177172184,
-0.0016292232321575284,
0.02819542959332466,
-0.08123597502708435,
0.040338508784770966,
-0.07299505174160004,
-0.00005267185406410135,
-0.035730987787246704,
0.04314722120761871,
0.049112822860479355,
-0.05116627737879753,
-0.04203201085329056,
-0.023556796833872795,
0.06566984206438065,
0.005103164352476597,
-0.06979258358478546,
0.017568198963999748,
-0.012869466096162796,
0.05099007487297058,
-0.0253745149821043,
0.018547840416431427,
0.021578704938292503,
0.013914481736719608,
-0.03918104246258736,
0.13309736549854279,
-0.04721027985215187,
0.009642391465604305,
-0.0027430986519902945,
0.0202998798340559,
0.042439136654138565,
0.016964441165328026,
-0.07259948551654816,
0.020390905439853668,
-0.03593752533197403,
-0.06573457270860672,
-0.06296691298484802,
0.030010497197508812,
-0.013232707045972347,
-0.0061029535718262196,
0.02542981319129467,
0.021011987701058388,
-0.057483378797769547,
0.007616365794092417,
-0.04223150014877319,
0.05354118347167969,
0.028862010687589645,
0.005940739065408707,
0.039287108927965164,
0.023732740432024002,
-0.00716225104406476,
0.03685183823108673,
0.03405201435089111,
-0.019235214218497276,
0.023274032399058342,
0.0017034178599715233,
0.052607111632823944,
-0.015291919000446796,
0.03977777063846588,
-0.022552458569407463,
-0.02689552865922451,
-0.02606230042874813,
-0.02547377161681652,
0.008656669408082962,
0.03371110185980797,
0.01649472676217556,
-0.09796877205371857,
0.08125532418489456,
0.04568147659301758,
0.005189472809433937,
0.08255991339683533,
-0.09632307291030884,
0.029948443174362183,
0.023605795577168465,
0.0465780608355999,
0.009966881945729256,
-0.0015723281539976597,
-0.06080791726708412,
-0.0010394837008789182,
0.01096242107450962,
-0.00396632170304656,
0.04864486679434776,
0.00810653530061245,
0.04511808604001999,
0.06508558243513107,
-0.007351314648985863,
0.014226735569536686,
-0.010855739936232567,
-0.007964747957885265,
0.05623703822493553,
-0.053568918257951736,
-0.06340091675519943,
-0.06881020963191986,
0.05629793182015419,
0.05545469745993614,
-0.24898158013820648,
0.019533302634954453,
0.00045041876728646457,
0.03196631371974945,
-0.034440845251083374,
0.023991091176867485,
0.028363315388560295,
-0.07231846451759338,
-0.052168965339660645,
0.02242189086973667,
0.06377416104078293,
0.049678172916173935,
0.04109502211213112,
-0.007067447993904352,
-0.013714880682528019,
-0.002130501437932253,
0.048542868345975876,
-0.032584965229034424,
0.09240999072790146,
0.00890346523374319,
0.011405798606574535,
-0.018664518371224403,
0.16816650331020355,
0.0954643189907074,
-0.023685745894908905,
0.05622230842709541,
-0.04974157735705376,
0.013240063562989235,
-0.03420981764793396,
-0.020225880667567253,
0.00605841726064682,
-0.024096781387925148,
0.08016884326934814,
-0.019403642043471336,
-0.018257010728120804,
0.08728689700365067,
0.002406598534435034,
-0.0420311838388443,
0.025153126567602158,
-0.008279728703200817,
0.02436181902885437,
0.034742116928100586,
-0.07220945507287979,
-0.0699428915977478,
0.0356454998254776,
-0.06955354660749435,
0.005181249231100082,
-0.056515950709581375,
0.04207689315080643,
-0.005234888754785061,
0.007795221172273159,
-0.05417994409799576,
-0.0020388660486787558,
-0.026880299672484398,
0.013487312942743301,
-0.0796535536646843,
-0.016433456912636757,
0.017638932913541794,
-0.03954140469431877,
0.007591952104121447,
0.028753330931067467,
-0.019594959914684296,
-0.04219023510813713,
0.0351751409471035,
0.0025531118735671043
] |
PMID:18616
|
Acute purulent otitis media in children older than 5 years. Incidence of Haemophilus as a causative organism.
|
It is generally believed that Haemophilus influenzae is not commonly a causative agent of otitis media in children older than 5 years of age. We recently studied cases of 58 children, aged from 5 to 9 years, who had acute otitis media. Haemophilus species were the causative agents in 36% of cases. This high incidence of Haemophilus isolation from the middle ear exudate of these children has important therapeutic implications; antibiotics effective against H influenzae should be employed when dealing with otitis media in this older age group.
|
Acute purulent otitis media in children older than 5 years. Incidence of Haemophilus as a causative organism. It is generally believed that Haemophilus influenzae is not commonly a causative agent of otitis media in children older than 5 years of age. We recently studied cases of 58 children, aged from 5 to 9 years, who had acute otitis media. Haemophilus species were the causative agents in 36% of cases. This high incidence of Haemophilus isolation from the middle ear exudate of these children has important therapeutic implications; antibiotics effective against H influenzae should be employed when dealing with otitis media in this older age group.
|
[
-0.046602651476860046,
-0.03557859733700752,
-0.02847186103463173,
-0.036972325295209885,
0.004877389874309301,
-0.019619211554527283,
0.04451807960867882,
0.08056558668613434,
-0.07352791726589203,
0.010395972058176994,
0.041110631078481674,
-0.07007219642400742,
0.01965705119073391,
0.03068448044359684,
0.004034269601106644,
-0.006176633294671774,
-0.0005023595876991749,
-0.05555921420454979,
-0.05930888280272484,
-0.0030030831694602966,
-0.013888558372855186,
0.03036791831254959,
0.053509850054979324,
-0.012071122415363789,
0.013911828398704529,
0.04829299822449684,
0.00005697882443200797,
-0.006386392284184694,
0.008598011918365955,
-0.1395210176706314,
0.02739172801375389,
-0.03254682943224907,
0.007675760425627232,
-0.012295529246330261,
-0.01933145523071289,
0.004441618453711271,
0.005071078892797232,
-0.02706693485379219,
-0.04146716743707657,
0.08954504132270813,
0.00008428349246969447,
0.08551209419965744,
-0.009404296986758709,
-0.05943598598241806,
-0.03658265247941017,
-0.026864759624004364,
-0.035842280834913254,
0.010366466827690601,
0.12525445222854614,
-0.03701765090227127,
-0.0003251431917306036,
0.017605137079954147,
-0.000254038255661726,
0.04432877525687218,
-0.03616505488753319,
0.0143657261505723,
0.014429481700062752,
0.009052754379808903,
0.003989510238170624,
0.04891008138656616,
0.033386461436748505,
0.025082401931285858,
-0.12070496380329132,
0.07348910719156265,
0.037829119712114334,
0.05487599968910217,
0.028588155284523964,
-0.01906837709248066,
-0.011607017368078232,
-0.05378060042858124,
-0.030633315443992615,
0.02048213593661785,
-0.0483124665915966,
0.06538205593824387,
0.026493873447179794,
-0.003128794487565756,
-0.0004482832446228713,
-0.09238313138484955,
0.032329391688108444,
0.007123028393834829,
-0.01050320640206337,
0.0630437582731247,
0.017267588526010513,
0.017361920326948166,
0.014161049388349056,
-0.023284900933504105,
0.000029082799301249906,
-0.0805138498544693,
-0.012523640878498554,
0.0018595032161101699,
-0.027971643954515457,
0.01798412576317787,
-0.06462450325489044,
0.04039331153035164,
0.008744779974222183,
-0.016026854515075684,
0.022581418976187706,
0.00865856371819973,
-0.029328390955924988,
0.3988327085971832,
-0.08206202834844589,
-0.08093947917222977,
-0.03377153351902962,
-0.022912081331014633,
0.03582819551229477,
-0.01279707532376051,
0.033553700894117355,
0.0038860319182276726,
0.022367598488926888,
0.051115259528160095,
-0.008551926352083683,
0.019618229940533638,
0.011527711525559425,
-0.03004557080566883,
-0.02912944369018078,
-0.0056280773133039474,
0.05515775829553604,
0.06043925881385803,
-0.04976881295442581,
0.022094860672950745,
-0.003176346654072404,
-0.015224763192236423,
0.03521489351987839,
0.01064108032733202,
0.03503718599677086,
-0.0017908659065142274,
0.015760023146867752,
0.08889171481132507,
0.04662599414587021,
0.0005260436446405947,
0.0733758881688118,
-0.04205115884542465,
-0.021367698907852173,
-0.0017346310196444392,
0.03829362988471985,
-0.04261254146695137,
-0.013219796121120453,
-0.04042345657944679,
-0.00666226539760828,
-0.04305922985076904,
-0.04509979113936424,
-0.12587293982505798,
-0.03305812180042267,
-0.046429529786109924,
-0.07137201726436615,
0.10954909771680832,
-0.06730403751134872,
0.08029536157846451,
-0.05546215921640396,
-0.03224508464336395,
-0.014260027557611465,
0.06305880099534988,
0.018656957894563675,
0.02187066152691841,
0.022592179477214813,
0.003130907192826271,
0.009970974177122116,
-0.04348938539624214,
-0.010587025433778763,
-0.0136806545779109,
-0.031873852014541626,
-0.00485615711659193,
-0.011856396682560444,
0.08142557740211487,
-0.062401413917541504,
0.005779345519840717,
-0.04098019003868103,
0.0053131612949073315,
0.06272465735673904,
0.020326590165495872,
-0.02075982093811035,
-0.008342930115759373,
0.03641266003251076,
0.013514118269085884,
-0.03156277537345886,
0.04371695965528488,
0.0006555758300237358,
-0.007359003182500601,
0.005769994109869003,
0.01643035002052784,
-0.04141983017325401,
0.041674066334962845,
-0.015044187195599079,
0.03875522315502167,
0.02355457842350006,
-0.03136547654867172,
-0.034411702305078506,
0.016811426728963852,
0.07677203416824341,
-0.027083229273557663,
-0.009341455064713955,
-0.05088306590914726,
-0.005375226028263569,
0.029949171468615532,
-0.01758592389523983,
0.03151388838887215,
-0.09919198602437973,
-0.03908570855855942,
0.0921555906534195,
-0.0011605166364461184,
-0.006708793807774782,
-0.001934846630319953,
-0.013073527254164219,
0.008913404308259487,
0.025554316118359566,
-0.006921906024217606,
0.01113060861825943,
-0.00933822151273489,
0.058364853262901306,
-0.009266831912100315,
0.011479600332677364,
0.017039252445101738,
-0.06548839062452316,
0.013394355773925781,
-0.01661539077758789,
0.07936371117830276,
-0.025136973708868027,
0.039313334971666336,
0.059881266206502914,
0.028812658041715622,
0.01375663559883833,
-0.05608043447136879,
0.022353066131472588,
-0.27284419536590576,
0.009057180024683475,
-0.01042250171303749,
-0.08868724852800369,
-0.0037336223758757114,
0.008091566152870655,
0.037854988127946854,
-0.048614807426929474,
0.021312426775693893,
-0.005476584192365408,
0.017951523885130882,
0.05142326280474663,
-0.07174569368362427,
-0.03989342600107193,
-0.03789674863219261,
-0.01319398544728756,
0.043657489120960236,
-0.01574118621647358,
0.043785709887742996,
0.026090513914823532,
0.05993060767650604,
-0.06400524824857712,
0.024714242666959763,
0.04405457526445389,
0.048387959599494934,
0.02670428343117237,
0.12040776014328003,
0.004715994466096163,
-0.018986083567142487,
0.024767901748418808,
0.0671507939696312,
-0.013795331120491028,
-0.0173051618039608,
-0.04332008212804794,
-0.0358855240046978,
0.012504903599619865,
0.014212566427886486,
-0.024952929466962814,
-0.03535575792193413,
-0.0393277183175087,
-0.07176635414361954,
0.02039213851094246,
-0.02476571314036846,
-0.022518523037433624,
-0.024380967020988464,
-0.041740015149116516,
0.032961007207632065,
0.0054832929745316505,
0.04188953712582588,
-0.014865278266370296,
0.06354805082082748,
-0.01835794188082218,
0.011776570230722427,
0.0800856426358223,
-0.03183286264538765,
-0.019139522686600685,
-0.003163568675518036,
0.016814369708299637,
-0.08521442115306854,
0.0219415295869112,
-0.01318227406591177,
0.03505176678299904,
0.02542286366224289,
-0.01719548925757408,
-0.07714691758155823,
-0.03705202415585518,
0.0005453453049995005,
-0.06925187259912491,
-0.03173918277025223,
0.048623185604810715,
-0.045627254992723465,
0.16006015241146088,
-0.03376859426498413,
-0.0621105395257473,
-0.0715872198343277,
0.0692954957485199,
-0.006482474971562624,
-0.037847232073545456,
0.025628268718719482,
0.04714038223028183,
0.03293897956609726,
-0.05814552679657936,
0.010442232713103294,
0.018150635063648224,
0.023143455386161804,
-0.0255484189838171,
-0.06690811365842819,
0.05698161572217941,
-0.011104540899395943,
-0.08773784339427948,
0.04985121265053749,
-0.0031367989722639322,
-0.04607441648840904,
0.02765197865664959,
-0.020181890577077866,
-0.0332355760037899,
-0.24123463034629822,
0.014112185686826706,
0.03795464336872101,
0.02021392248570919,
-0.025782467797398567,
0.020125392824411392,
-0.01150711253285408,
-0.07140249758958817,
0.014260726980865002,
0.01980905421078205,
0.036982033401727676,
-0.018607651814818382,
0.04661601409316063,
-0.005356376990675926,
-0.010445539839565754,
0.011442125774919987,
0.019469909369945526,
-0.00624014250934124,
0.03351817652583122,
0.015304120257496834,
0.025581225752830505,
-0.026027237996459007,
0.1542864739894867,
0.00662519596517086,
-0.009490524418652058,
0.024817096069455147,
0.012122748419642448,
0.033891137689352036,
0.00723305344581604,
-0.019568493589758873,
0.06252112239599228,
-0.014748260378837585,
0.11035417765378952,
-0.0008973211515694857,
0.005491508636623621,
-0.03044964373111725,
-0.028674231842160225,
-0.015502388589084148,
-0.027011390775442123,
0.017468038946390152,
-0.028252728283405304,
0.02543431706726551,
-0.01847901940345764,
-0.02868553251028061,
0.04866928234696388,
0.016346152871847153,
0.01785816252231598,
-0.07587973773479462,
0.02777809649705887,
0.011910775676369667,
-0.021630458533763885,
-0.07463253289461136,
0.08238081634044647,
0.029309460893273354,
0.07578452676534653,
-0.04837362468242645,
-0.009358822368085384,
-0.001724585541523993,
-0.012973729521036148,
-0.04942856729030609,
0.043666135519742966,
-0.05182874575257301,
-0.056487102061510086,
0.11543106287717819,
0.041031885892152786
] |
PMID:18618
|
Severe combined immunodeficiency disease. Characterization of the disease and results of transplantation.
|
Pretransplant and posttransplant data for 69 patients with severe combined immunodeficiency disease are presented. Both B and T lymphocyte functions were absent in approximately 80% of the children and markedly depressed in the remainder. Transplantation of marrow from HLA genotypically identical donors provided the highest six-month survival rate (63%); six-month survival rates for patients who received fetal tissue transplants (43%) or marrow from mixed leukocyte culture (MLC) negative donors (38%) were significantly higher (P less than .05) than for patients treated with marrow from MLC positive donors (5%). Additional factors appeared to influence survival and the severity of graft-vs-host (GVH) disease. Patients more than 6 months of age had more intense GVH disease than younger patients. Survival rates were lower and GVH disease more intense when boys received transplants from girl donors than the reverse.
|
Severe combined immunodeficiency disease. Characterization of the disease and results of transplantation. Pretransplant and posttransplant data for 69 patients with severe combined immunodeficiency disease are presented. Both B and T lymphocyte functions were absent in approximately 80% of the children and markedly depressed in the remainder. Transplantation of marrow from HLA genotypically identical donors provided the highest six-month survival rate (63%); six-month survival rates for patients who received fetal tissue transplants (43%) or marrow from mixed leukocyte culture (MLC) negative donors (38%) were significantly higher (P less than .05) than for patients treated with marrow from MLC positive donors (5%). Additional factors appeared to influence survival and the severity of graft-vs-host (GVH) disease. Patients more than 6 months of age had more intense GVH disease than younger patients. Survival rates were lower and GVH disease more intense when boys received transplants from girl donors than the reverse.
|
[
-0.013829863630235195,
0.007003354839980602,
0.018769938498735428,
0.003092530183494091,
-0.03718859329819679,
0.0016571307787671685,
0.020439138635993004,
0.10861364752054214,
-0.04017342999577522,
0.0334685817360878,
0.027965916320681572,
-0.029591651633381844,
0.038892872631549835,
-0.06058042123913765,
-0.05144226551055908,
0.057167354971170425,
0.03055536188185215,
-0.005839873570948839,
-0.07970163226127625,
0.03697473555803299,
-0.04920252040028572,
-0.018122097477316856,
0.013785898685455322,
-0.03699095547199249,
0.10882609337568283,
0.0061141191981732845,
0.011604632250964642,
-0.02076171711087227,
-0.012638352811336517,
-0.18866415321826935,
0.007363369222730398,
-0.01441341731697321,
-0.03190663456916809,
-0.03031112253665924,
-0.0061756013892591,
-0.01715337298810482,
0.000798557884991169,
0.03097832016646862,
-0.011443110182881355,
0.0318155363202095,
0.014559920877218246,
0.028081966564059258,
-0.051662638783454895,
0.03636447340250015,
0.018203577026724815,
-0.0021072328090667725,
-0.07353441417217255,
-0.0014209278160706162,
0.08221760392189026,
0.04402536898851395,
0.019269024953246117,
-0.014699653722345829,
0.034636240452528,
0.10186593234539032,
0.007955060340464115,
0.025576358661055565,
0.03229088336229324,
0.003254903247579932,
0.0037489559035748243,
0.014989631250500679,
-0.016880203038454056,
0.0455259308218956,
-0.12036076933145523,
0.055281128734350204,
0.07911952584981918,
-0.04123988375067711,
-0.05532797425985336,
-0.09590232372283936,
-0.012399023398756981,
0.03820363059639931,
-0.052268289029598236,
0.012171786278486252,
0.03583445027470589,
0.045640405267477036,
-0.053295399993658066,
-0.006614498794078827,
-0.020604372024536133,
-0.03845067322254181,
0.04258442297577858,
0.038973379880189896,
-0.007397645618766546,
0.04246455430984497,
0.00203731469810009,
-0.03330360725522041,
-0.012052841484546661,
-0.05031108483672142,
-0.054898664355278015,
-0.03795807063579559,
-0.014616253785789013,
0.001508095534518361,
0.013542300090193748,
0.009999255649745464,
-0.021225756034255028,
0.00514679029583931,
-0.06444185972213745,
0.045665282756090164,
-0.030943894758820534,
-0.060304880142211914,
0.005236294586211443,
0.441500723361969,
0.013922587037086487,
-0.011721428483724594,
-0.02103189192712307,
0.008741730824112892,
-0.02169785089790821,
-0.020221319049596786,
0.010190294124186039,
-0.04625539854168892,
-0.06997869163751602,
0.01839444413781166,
-0.03781282901763916,
0.007226620800793171,
0.06057821959257126,
-0.028703557327389717,
-0.04682689532637596,
0.006611700635403395,
-0.0026281389873474836,
-0.007765448652207851,
0.0013392562977969646,
0.031627096235752106,
0.015077690593898296,
-0.002831449033692479,
0.04357330873608589,
-0.051161203533411026,
0.015072719193994999,
0.026508457958698273,
0.02439960464835167,
0.06517153978347778,
0.032917797565460205,
-0.00027879010303877294,
0.0563894659280777,
0.06230558082461357,
-0.019365893676877022,
0.024438362568616867,
-0.016579685732722282,
0.011338257230818272,
0.030087027698755264,
-0.0004686511238105595,
-0.00015076255658641458,
-0.0027888973709195852,
0.06802759319543839,
-0.0550084225833416,
-0.004047654569149017,
-0.041799068450927734,
-0.050384849309921265,
-0.003691905876621604,
-0.01731276512145996,
-0.008844100870192051,
0.025299493223428726,
0.03748176246881485,
0.030853455886244774,
0.07711899280548096,
0.07234573364257812,
-0.016339555382728577,
-0.017855608835816383,
-0.005042878910899162,
-0.04789426177740097,
0.0031247774604707956,
-0.02112792246043682,
0.011193746700882912,
-0.007427682634443045,
-0.027534695342183113,
-0.02278652973473072,
0.10155385732650757,
-0.017102301120758057,
0.01823822222650051,
-0.026946574449539185,
-0.00818402785807848,
0.05004280060529709,
0.027067987248301506,
0.02974667027592659,
0.054093483835458755,
0.04420121759176254,
-0.05521056428551674,
0.0074233259074389935,
-0.055050913244485855,
-0.0682060644030571,
0.009231960400938988,
0.01167949941009283,
0.03898365795612335,
-0.043394215404987335,
0.01980503275990486,
-0.03483184427022934,
0.024192560464143753,
0.06588607281446457,
0.01911379210650921,
-0.040690165013074875,
0.023619474843144417,
0.0446976013481617,
0.02880179136991501,
0.00003315123831271194,
-0.023126062005758286,
-0.07933457940816879,
-0.018841532990336418,
0.07586304843425751,
-0.01726006530225277,
-0.03662257641553879,
-0.05004359781742096,
0.03953174874186516,
-0.026166796684265137,
0.06060117483139038,
-0.03168225660920143,
-0.0012713258620351553,
-0.024817872792482376,
0.03758077695965767,
0.004648217000067234,
0.010408592410385609,
-0.045394159853458405,
0.03893337398767471,
0.038562871515750885,
0.004392918199300766,
-0.023730503395199776,
-0.013495247811079025,
0.046090804040431976,
-0.02818319946527481,
0.017049938440322876,
-0.03323211148381233,
0.023677848279476166,
0.020364776253700256,
0.014774642884731293,
0.004856743849813938,
-0.013236337341368198,
-0.03516675531864166,
-0.3121818006038666,
-0.05785198137164116,
-0.0116750942543149,
0.007963001728057861,
-0.010683472268283367,
-0.02274966426193714,
0.0021584676578640938,
-0.0429021418094635,
-0.01983136124908924,
0.03273182362318039,
0.11090777814388275,
0.08191730827093124,
-0.06255123019218445,
-0.007342308759689331,
-0.027488650754094124,
-0.009190605953335762,
0.025028392672538757,
0.006182595621794462,
-0.0037642286624759436,
-0.006288236938416958,
0.005253090523183346,
0.03364060819149017,
0.051404621452093124,
0.016980206593871117,
0.03229702264070511,
0.010483715683221817,
0.1262732446193695,
-0.03094254806637764,
-0.010035342536866665,
-0.01249589491635561,
0.013486572541296482,
0.02837512269616127,
-0.028018265962600708,
-0.03242570906877518,
0.03592440485954285,
-0.035319387912750244,
-0.0009075517882592976,
-0.03137611597776413,
0.007763809058815241,
0.026416316628456116,
-0.05688796192407608,
0.002806101692840457,
-0.01235613226890564,
-0.08807047456502914,
0.0068211001344025135,
0.04411923140287399,
-0.010136476717889309,
0.04105554148554802,
-0.03293129801750183,
0.02035805769264698,
0.043327175080776215,
-0.04568140208721161,
0.046531885862350464,
0.055451199412345886,
0.009910575114190578,
0.032419223338365555,
-0.06342042237520218,
-0.009469554759562016,
-0.06461771577596664,
-0.008606654591858387,
-0.010293605737388134,
-0.008376638405025005,
0.030839402228593826,
-0.017514606937766075,
-0.05508030205965042,
0.0009184231166727841,
0.03786448761820793,
0.0549372173845768,
0.026412155479192734,
0.0012311891186982393,
-0.01981031708419323,
0.02159164473414421,
-0.059233736246824265,
0.027703700587153435,
0.01918509230017662,
0.010340149514377117,
-0.058922089636325836,
-0.05154988169670105,
-0.059593312442302704,
-0.009942698292434216,
0.007466200273483992,
-0.03641394153237343,
0.0009724028059281409,
0.0476575568318367,
0.06555736809968948,
0.03714757412672043,
-0.00640662107616663,
0.03355924040079117,
-0.032482389360666275,
0.011576777324080467,
-0.016114382073283195,
-0.04096858203411102,
0.002567620249465108,
-0.018559329211711884,
-0.06467311084270477,
0.03688140586018562,
-0.2560727596282959,
0.00817772839218378,
0.01235236693173647,
-0.06022091209888458,
-0.07583019137382507,
-0.0017474183114245534,
0.003519789082929492,
-0.07151468098163605,
-0.02566957287490368,
0.028913453221321106,
0.08694729208946228,
0.001917279907502234,
0.13343378901481628,
-0.03983829915523529,
0.012020668014883995,
0.007018126547336578,
0.024018866941332817,
-0.026789702475070953,
-0.03118457831442356,
-0.009902151301503181,
0.037259481847286224,
-0.004900848027318716,
0.1006002277135849,
-0.02728603221476078,
-0.017514534294605255,
0.011318396776914597,
-0.010227957740426064,
0.06497552990913391,
-0.035623956471681595,
-0.053035374730825424,
0.020440341904759407,
0.027390189468860626,
0.05632564425468445,
0.016772868111729622,
0.04041137918829918,
0.06245690956711769,
-0.033243026584386826,
0.03987772762775421,
0.015944303944706917,
0.06574681401252747,
-0.03848447650671005,
0.0009459726279601455,
0.03260282427072525,
0.03546607494354248,
0.04700443521142006,
-0.0011395487235859036,
-0.0023704420309513807,
-0.07792015373706818,
-0.055150337517261505,
-0.01026800274848938,
-0.06738638132810593,
0.02272157184779644,
0.042100660502910614,
-0.050132423639297485,
0.001970533048734069,
0.011707072146236897,
0.027270769700407982,
-0.03446545451879501,
0.039970338344573975,
-0.03873526304960251,
0.035826608538627625,
-0.029728654772043228,
-0.08386591076850891,
0.07854235917329788,
-0.0863439291715622
] |
PMID:18620
|
Nephropathia epidemica. The Scandinavian form of hemorrhagic fever with renal syndrome.
|
We report epidemiologic, clinical, laboratory, and biopsy findings in 14 cases of nephropathia epidemica. The patients were between 19 and 49 years of age. The onset of the disease was characterized by high fever, nausea, headache, backache, abdominal pain, proteinuria, oliguria, hematuria, and uremia. The symptoms subsided rapidly during the polyuria phase, which followed the oliguria stage. Because of renal failure, hemodialysis was required in eight cases. Edema of eyelids, conjunctival injection and hemorrhages, transitory myopia, and acute glaucoma were the most common eye abnormalities. Renal biopsy specimens showed glomerular changes, with mild swelling of the epithelial cells of Bowman's capsule, thickening of the basement membrane of glomerular capillaries, glomerular adhesions, inflammatory cell infiltration, leukocytoclasis and hemorrhages in the interstitium, and eosinophilic hyaline degeneration and vacuolization of the epithelial cells of the proximal tubuli.
|
Nephropathia epidemica. The Scandinavian form of hemorrhagic fever with renal syndrome. We report epidemiologic, clinical, laboratory, and biopsy findings in 14 cases of nephropathia epidemica. The patients were between 19 and 49 years of age. The onset of the disease was characterized by high fever, nausea, headache, backache, abdominal pain, proteinuria, oliguria, hematuria, and uremia. The symptoms subsided rapidly during the polyuria phase, which followed the oliguria stage. Because of renal failure, hemodialysis was required in eight cases. Edema of eyelids, conjunctival injection and hemorrhages, transitory myopia, and acute glaucoma were the most common eye abnormalities. Renal biopsy specimens showed glomerular changes, with mild swelling of the epithelial cells of Bowman's capsule, thickening of the basement membrane of glomerular capillaries, glomerular adhesions, inflammatory cell infiltration, leukocytoclasis and hemorrhages in the interstitium, and eosinophilic hyaline degeneration and vacuolization of the epithelial cells of the proximal tubuli.
|
[
-0.007496587932109833,
-0.013665194623172283,
0.025025272741913795,
0.007354654371738434,
0.006673510652035475,
-0.03683191537857056,
0.08919362723827362,
0.05775226652622223,
-0.004534941166639328,
0.021658439189195633,
0.0019142875680699944,
-0.05917951464653015,
0.009143315255641937,
0.036426350474357605,
-0.06641257554292679,
-0.01193102728575468,
-0.024235762655735016,
-0.011522650718688965,
-0.001272330991923809,
0.04658661410212517,
-0.021153105422854424,
-0.03924952447414398,
0.022793836891651154,
-0.0018012808868661523,
0.026866616681218147,
0.019886290654540062,
0.047463834285736084,
0.0647408589720726,
-0.0275895819067955,
-0.15289172530174255,
0.019222278147935867,
-0.038859233260154724,
-0.03685064613819122,
-0.007761105895042419,
-0.04503050446510315,
-0.019221115857362747,
-0.06350590288639069,
0.009009691886603832,
-0.004966304171830416,
0.010280736722052097,
-0.019879069179296494,
0.01616032049059868,
-0.016730954870581627,
-0.01656610146164894,
-0.023391272872686386,
0.007297462318092585,
-0.09189452975988388,
0.07535732537508011,
0.1396774798631668,
0.014700483530759811,
-0.06869981437921524,
-0.049693513661623,
0.020516566932201385,
0.06524144113063812,
0.009540531784296036,
-0.06947658956050873,
0.0029812606517225504,
0.00041758830775506794,
0.07307470589876175,
0.038965482264757156,
0.05906302481889725,
-0.036754511296749115,
-0.10104406625032425,
0.08346689492464066,
0.029883703216910362,
-0.022378791123628616,
0.00300217280164361,
-0.022958016023039818,
0.01718473806977272,
-0.03250027447938919,
-0.05565410852432251,
0.08994946628808975,
0.0005877610528841615,
0.0347633920609951,
-0.02087973617017269,
-0.0027723449748009443,
-0.0323173813521862,
0.028728485107421875,
0.048803605139255524,
0.05466025695204735,
0.003760362509638071,
0.06811871379613876,
0.09858954697847366,
-0.0471264086663723,
0.05059387534856796,
-0.0572299025952816,
-0.021637404337525368,
0.013182499445974827,
-0.012417850084602833,
-0.03175892308354378,
-0.023165278136730194,
-0.007630273699760437,
-0.018025372177362442,
0.008899628184735775,
-0.05927792936563492,
0.0227111354470253,
-0.016525108367204666,
0.00346853444352746,
-0.034422677010297775,
0.40367594361305237,
-0.01964196190237999,
-0.018683796748518944,
0.019935663789510727,
0.02306823991239071,
0.022205568850040436,
-0.013518055900931358,
0.02045436203479767,
-0.029167398810386658,
-0.016299718990921974,
0.057945702224969864,
0.020125525072216988,
-0.0074163093231618404,
0.0433211512863636,
-0.059585195034742355,
0.0003625662939157337,
-0.03492160886526108,
0.09922516345977783,
-0.041256073862314224,
-0.06831815093755722,
0.08140991628170013,
0.02412419393658638,
0.02089296281337738,
0.00809501949697733,
-0.021421188488602638,
0.05286882817745209,
0.06703697144985199,
0.054252222180366516,
0.08421891182661057,
0.049212854355573654,
-0.005277820397168398,
-0.016068480908870697,
0.02303115837275982,
-0.019073564559221268,
-0.02437492460012436,
0.006935799960047007,
-0.0057358224876224995,
0.029990579932928085,
-0.0639008954167366,
0.02534578926861286,
0.0459769181907177,
-0.01014374103397131,
-0.11103015393018723,
-0.06107456237077713,
-0.057319335639476776,
-0.07048197835683823,
0.09657935053110123,
0.0013332261005416512,
0.004254477098584175,
-0.025147274136543274,
-0.03385668620467186,
-0.00220104749314487,
0.055275075137615204,
-0.0012878611451014876,
-0.011384369805455208,
0.01552884466946125,
0.00843537226319313,
-0.021463796496391296,
0.029627561569213867,
-0.031029116362333298,
-0.03537860885262489,
-0.022926537320017815,
-0.07334936410188675,
0.014283712022006512,
-0.02057844027876854,
0.028063463047146797,
-0.022554414346814156,
-0.012432635761797428,
-0.04982398450374603,
0.02440943755209446,
0.03434256464242935,
0.017783446237444878,
0.004517405293881893,
0.03759448975324631,
0.004027658142149448,
0.005816442426294088,
-0.008750329725444317,
-0.01591106317937374,
-0.04209991171956062,
-0.037092581391334534,
-0.01484163198620081,
0.014665510505437851,
0.0024431271012872458,
-0.017977679148316383,
0.048914942890405655,
0.050061263144016266,
-0.06263962388038635,
-0.048363570123910904,
0.03861656039953232,
0.052195049822330475,
-0.05329662933945656,
-0.03464674949645996,
-0.02726875990629196,
-0.04814523458480835,
0.004070843104273081,
0.038325611501932144,
0.020365620031952858,
-0.05603070929646492,
0.006419795099645853,
-0.0011874096235260367,
-0.06167009100317955,
0.06998801976442337,
0.015004637651145458,
0.006386372726410627,
0.0016653252532705665,
-0.019444096833467484,
-0.0075770895928144455,
0.021899348124861717,
0.0037063932977616787,
-0.034892041236162186,
-0.022393329069018364,
0.0027675279416143894,
0.018982820212841034,
-0.017210306599736214,
0.019197823479771614,
0.01868215575814247,
0.018646525219082832,
-0.06378105282783508,
-0.015017838217318058,
0.04415540769696236,
-0.01462284754961729,
0.061876941472291946,
-0.01000953558832407,
-0.018612632527947426,
-0.28132715821266174,
-0.025568589568138123,
0.022544384002685547,
-0.03239793702960014,
0.022900907322764397,
-0.038103535771369934,
0.007365644443780184,
-0.041120272129774094,
0.056747179478406906,
0.03202636167407036,
0.0796908512711525,
0.09178891032934189,
0.011805038899183273,
-0.057108499109745026,
-0.013637990690767765,
0.05997033789753914,
0.031971752643585205,
0.03884222358465195,
-0.023421594873070717,
0.025199057534337044,
0.03946749493479729,
-0.034401509910821915,
0.02045261301100254,
0.04289676994085312,
0.03366134688258171,
-0.02400173991918564,
0.11333166062831879,
0.04672595113515854,
-0.08325797319412231,
-0.08212801814079285,
0.017162349075078964,
0.030283408239483833,
-0.01444636844098568,
-0.0736076608300209,
0.025975897908210754,
-0.008494353853166103,
0.000059196627262281254,
-0.04131688177585602,
-0.011223424226045609,
-0.014228932559490204,
-0.05242074653506279,
-0.032013993710279465,
-0.03807517886161804,
0.04616571217775345,
-0.031031204387545586,
0.00501942029222846,
0.0201738178730011,
0.021693004295229912,
0.01866157352924347,
0.021839134395122528,
0.03533715382218361,
-0.03425034135580063,
0.005023422185331583,
-0.00444867042824626,
0.003467814065515995,
-0.03363896161317825,
-0.08897217363119125,
-0.030659914016723633,
-0.06340973824262619,
-0.033103737980127335,
0.02235369198024273,
0.050063133239746094,
0.07933264225721359,
-0.09140772372484207,
-0.03975774720311165,
-0.011494302190840244,
0.02965768240392208,
0.0036292043514549732,
0.027486583217978477,
0.04641871154308319,
-0.03410816192626953,
0.1284545511007309,
-0.021369528025388718,
0.0645614042878151,
0.045381851494312286,
0.03629695251584053,
-0.017422853037714958,
-0.03544143587350845,
-0.009343313053250313,
0.04214337840676308,
-0.010149726644158363,
-0.08809250593185425,
0.005513425450772047,
0.026677457615733147,
0.03103974089026451,
0.03290789574384689,
-0.027356456965208054,
-0.009280960075557232,
0.01534232310950756,
-0.0021712181624025106,
0.0023441421799361706,
-0.03732364624738693,
-0.0961105152964592,
0.01339775137603283,
-0.04939788579940796,
0.06334210932254791,
-0.2897867262363434,
0.002803224604576826,
-0.027443179860711098,
0.022769873961806297,
-0.009207713417708874,
0.02275438793003559,
-0.09057991206645966,
-0.031090179458260536,
-0.0053261276334524155,
-0.002745389938354492,
0.04857838898897171,
0.02023172378540039,
0.07305951416492462,
0.005696895066648722,
-0.017765549942851067,
0.0490911602973938,
0.033936191350221634,
-0.012867056764662266,
0.039027296006679535,
-0.03687816858291626,
0.0010471382411196828,
-0.003511220682412386,
0.12430093437433243,
-0.0032066514249891043,
-0.03785529360175133,
0.02557566575706005,
0.048647183924913406,
0.04474354535341263,
-0.10760097205638885,
-0.01228597667068243,
0.03993033617734909,
0.01685374230146408,
0.04808768630027771,
0.009488233365118504,
0.07459673285484314,
-0.007549253758043051,
0.004986201412975788,
0.027585020288825035,
-0.0220637284219265,
0.03442411869764328,
-0.029360894113779068,
0.07043199241161346,
-0.02212953194975853,
0.011068165302276611,
-0.00020768911053892225,
-0.014318245463073254,
-0.03986513242125511,
-0.04217610880732536,
0.014195375144481659,
0.020385636016726494,
0.047871507704257965,
-0.032186511904001236,
0.020414983853697777,
0.02037208154797554,
-0.0028082281351089478,
0.0009351570042781532,
-0.030521823093295097,
-0.03557135909795761,
0.013764210976660252,
-0.056994739919900894,
-0.007612556219100952,
0.03747004270553589,
-0.04812086373567581,
-0.00779595086351037,
-0.03299323096871376
] |
PMID:18623
|
Pharmacologic analysis of stretch-induced sinus acceleration of the isolated dog atrium.
|
Using isolated, blood-perfused atrium preparation of the dogs, the effect of stretch on sinus rate was studied in 11 preparations. Stretching usually produced sinus acceleration in all 11 spontaneously beating atria. Within a range of 0--30 Gm, greater degrees of stretch produced greater degrees of sinus acceleration. During maintained stretch sinus rate slightly reduced but not to the control rate. On release from stretch sinus rate immediately returned to the control rate, and there was occasionally a slowing to rates lower than control in verapamil treated preparations. Atropine, adrenergic beta-receptor blocking agents, propranolol and carteolol, verapamil, and tetrodotoxin did not greatly modify responses to stretching.
|
Pharmacologic analysis of stretch-induced sinus acceleration of the isolated dog atrium. Using isolated, blood-perfused atrium preparation of the dogs, the effect of stretch on sinus rate was studied in 11 preparations. Stretching usually produced sinus acceleration in all 11 spontaneously beating atria. Within a range of 0--30 Gm, greater degrees of stretch produced greater degrees of sinus acceleration. During maintained stretch sinus rate slightly reduced but not to the control rate. On release from stretch sinus rate immediately returned to the control rate, and there was occasionally a slowing to rates lower than control in verapamil treated preparations. Atropine, adrenergic beta-receptor blocking agents, propranolol and carteolol, verapamil, and tetrodotoxin did not greatly modify responses to stretching.
|
[
-0.0364839993417263,
0.04453994706273079,
0.05293666571378708,
0.09741579741239548,
0.0028407226782292128,
0.025128304958343506,
0.008890299126505852,
0.009034198708832264,
-0.0059390258975327015,
-0.028313182294368744,
0.006562284659594297,
-0.01816452108323574,
-0.042872264981269836,
0.059315748512744904,
-0.025544868782162666,
-0.02624977007508278,
0.028532126918435097,
-0.0012395409867167473,
-0.04716763645410538,
-0.00632703909650445,
-0.03660016134381294,
0.01215544156730175,
0.04656558111310005,
-0.007499507628381252,
0.015127508901059628,
0.03210711479187012,
-0.04637998715043068,
-0.021982312202453613,
-0.03829050809144974,
-0.15560883283615112,
0.0016584013355895877,
0.032442428171634674,
0.05588793382048607,
-0.03962719812989235,
0.020785445347428322,
0.01954391598701477,
-0.0706275999546051,
0.03349563851952553,
0.02380264364182949,
0.07328256964683533,
0.02999219484627247,
0.016554173082113266,
-0.03380192443728447,
0.04100675508379936,
0.026896053925156593,
0.03158549964427948,
-0.051746923476457596,
-0.0003999928885605186,
0.06814707815647125,
0.04014524072408676,
0.014310884289443493,
-0.025992218405008316,
0.0070236846804618835,
0.02559293620288372,
-0.010420299135148525,
-0.0522899404168129,
0.007994144223630428,
0.04461846500635147,
0.01351195015013218,
0.020719559863209724,
-0.0011090427869930863,
0.06206868216395378,
-0.11245116591453552,
0.04907434061169624,
-0.017532827332615852,
0.02203533612191677,
-0.0014594978420063853,
0.04637186974287033,
-0.015787770971655846,
0.10470107197761536,
-0.01756054162979126,
0.04190487414598465,
-0.0032039156649261713,
-0.030957195907831192,
0.041470300406217575,
0.011597679927945137,
-0.04766244813799858,
-0.03566506877541542,
0.010949501767754555,
0.060293909162282944,
0.0165241751819849,
-0.04182323440909386,
-0.007352401036769152,
-0.05652347952127457,
0.023282013833522797,
-0.002479896415024996,
0.015921682119369507,
-0.04485820233821869,
0.01641327142715454,
0.0040057930164039135,
-0.038624607026576996,
-0.04516512155532837,
-0.007465878501534462,
-0.023220615461468697,
0.0003363946743775159,
0.0004350177478045225,
-0.034788668155670166,
0.05181394889950752,
-0.04576506093144417,
0.37908628582954407,
0.037087660282850266,
0.08368133753538132,
-0.069192074239254,
0.02622123993933201,
0.02533290907740593,
-0.046191368252038956,
0.05170135945081711,
0.03134787827730179,
-0.0377044677734375,
0.057821471244096756,
0.021761585026979446,
-0.03865509852766991,
-0.009031850844621658,
-0.013374865986406803,
0.03060087189078331,
0.010380979627370834,
0.0018165676156058908,
0.0025236092042177916,
0.046247754245996475,
0.02234353870153427,
-0.09259475022554398,
0.012926869094371796,
0.0368734672665596,
-0.028721816837787628,
0.028861073777079582,
-0.01924004964530468,
-0.028402643278241158,
0.058744460344314575,
0.020945973694324493,
-0.052153460681438446,
0.04766981303691864,
-0.035624776035547256,
-0.026751386001706123,
-0.0061842347495257854,
0.0057177068665623665,
-0.03895163536071777,
0.03190107271075249,
-0.015599578619003296,
0.003977322019636631,
0.009431860409677029,
-0.045349374413490295,
-0.06462617218494415,
0.04061988741159439,
-0.13058334589004517,
-0.05923135206103325,
0.10564395040273666,
-0.03033076785504818,
0.02782597579061985,
-0.06102576479315758,
-0.011377958580851555,
-0.02871938794851303,
-0.014816350303590298,
-0.08466716855764389,
0.052727844566106796,
-0.03362807258963585,
-0.009790046140551567,
0.010230028070509434,
0.004969497676938772,
-0.05092693492770195,
-0.0016083549708127975,
-0.06687171757221222,
-0.04538504034280777,
-0.010942371562123299,
0.030515143647789955,
-0.013447794131934643,
-0.03811977431178093,
-0.025032293051481247,
0.06167292967438698,
0.0020807869732379913,
0.043934404850006104,
-0.03477395325899124,
-0.01819821260869503,
-0.0010222916025668383,
-0.023585036396980286,
0.01228809729218483,
0.02747243084013462,
0.029556836932897568,
-0.023807356134057045,
0.043506693094968796,
-0.02052919566631317,
0.052191443741321564,
0.0070818145759403706,
0.020163273438811302,
-0.04040016233921051,
-0.04558901861310005,
0.035132601857185364,
-0.07189662009477615,
-0.028332263231277466,
-0.0018338283989578485,
-0.001083831419236958,
0.008333826437592506,
-0.038105156272649765,
-0.09893705695867538,
0.012152430601418018,
-0.06565576791763306,
-0.026370538398623466,
0.02437039278447628,
0.016784312203526497,
-0.051849428564310074,
0.041206248104572296,
0.04458492621779442,
-0.07445134222507477,
-0.00514184171333909,
0.02782381884753704,
0.04088646173477173,
0.04849124699831009,
-0.040995221585035324,
0.01664387620985508,
0.0265643410384655,
0.02657611295580864,
0.03181380033493042,
0.012071928940713406,
0.07203758507966995,
-0.0164262093603611,
-0.06888695061206818,
0.09163003414869308,
-0.07641234993934631,
0.0355793796479702,
0.008977430872619152,
-0.019083186984062195,
0.04109133034944534,
-0.06019294634461403,
0.045368727296590805,
-0.31175342202186584,
-0.006592826452106237,
0.003282983787357807,
0.020256422460079193,
0.015512803569436073,
-0.010030964389443398,
0.061722178012132645,
-0.03480110689997673,
0.0031853653490543365,
-0.07787835597991943,
0.0036504187155514956,
0.06188991293311119,
-0.04497799277305603,
-0.03440878912806511,
-0.005241892766207457,
0.05716639384627342,
0.04208147153258324,
-0.004157268442213535,
0.026461675763130188,
-0.022958355024456978,
0.02884659357368946,
0.018963564187288284,
-0.026886524632573128,
0.03007325530052185,
-0.038065437227487564,
-0.007842150516808033,
0.11072816699743271,
-0.00784278940409422,
-0.007680836133658886,
-0.038326017558574677,
-0.021439336240291595,
0.008166719228029251,
0.029084697365760803,
-0.12195219099521637,
0.026307763531804085,
-0.023369459435343742,
0.07287447899580002,
0.026591001078486443,
0.015018478035926819,
0.001630287035368383,
-0.07299670577049255,
0.07762911170721054,
0.027267666533589363,
-0.013647441752254963,
-0.05997532606124878,
0.02280202880501747,
-0.010595792904496193,
0.03137531131505966,
0.005345364101231098,
0.004365349188446999,
0.04449053108692169,
-0.03408819064497948,
0.0055763013660907745,
-0.03568786010146141,
0.04071657359600067,
-0.0015177639434114099,
-0.08320687711238861,
-0.01100755576044321,
-0.029576966539025307,
-0.0168449804186821,
-0.02028845250606537,
-0.025407232344150543,
0.07198098301887512,
-0.03272923827171326,
-0.01227633934468031,
0.05252545699477196,
0.03439056873321533,
0.017332689836621284,
0.030721092596650124,
0.002891071140766144,
0.011909336782991886,
0.11542565375566483,
-0.04105960577726364,
0.0012984538916498423,
0.06872665137052536,
-0.008216312155127525,
-0.06780996918678284,
-0.051618266850709915,
-0.026780350133776665,
-0.01983947493135929,
0.021792486310005188,
0.035067956894636154,
-0.009048371575772762,
0.0175238698720932,
-0.05025488883256912,
-0.05326167866587639,
0.0254956167191267,
0.02176644839346409,
0.0670117735862732,
0.015748517587780952,
0.0008684154599905014,
0.010222071781754494,
-0.0005751376156695187,
-0.008718300610780716,
0.00033589679514989257,
-0.005388990510255098,
-0.2815868556499481,
-0.02945435792207718,
0.08745577931404114,
-0.04396000877022743,
-0.004340353887528181,
0.0364571250975132,
0.0352182574570179,
-0.0658637136220932,
-0.042421337217092514,
0.006387303117662668,
-0.06957800686359406,
0.017438435927033424,
0.05489271506667137,
0.07188022881746292,
-0.029355572536587715,
0.011692056432366371,
0.028376013040542603,
-0.022556491196155548,
0.027702022343873978,
-0.0309846680611372,
0.004485027864575386,
0.01352487038820982,
0.14382068812847137,
0.02770545892417431,
-0.05782289057970047,
0.0716073140501976,
0.023634066805243492,
0.022314462810754776,
0.05234689265489578,
0.006681398954242468,
0.02801109291613102,
-0.011785941198468208,
0.04933691769838333,
-0.021915055811405182,
0.027002695947885513,
-0.013050463050603867,
-0.013462488539516926,
0.029434572905302048,
0.013132656924426556,
-0.03140343725681305,
0.03157765045762062,
0.026951104402542114,
-0.048947229981422424,
-0.0641631931066513,
0.04730447754263878,
-0.08461426198482513,
-0.0826503336429596,
-0.021528607234358788,
-0.013403349556028843,
0.01605161838233471,
-0.039096564054489136,
-0.010720820166170597,
0.09164892882108688,
0.03838212043046951,
-0.013922580517828465,
-0.032550666481256485,
0.0053170220926404,
-0.04327791929244995,
0.029757864773273468,
-0.03783515840768814,
-0.05845100060105324,
-0.02986183390021324,
-0.0027268219273537397,
0.05443117022514343,
0.08940884470939636
] |
PMID:18629
|
A comparative study of the effects of OPC-1085 and propranolol on isolated guinea pig atrium.
|
Effects of a newly synthesized beta-blocker, 5-(3-tert-butylamino-2-hydroxy) propoxy-3,4-dihydrocarbostyril (OPC-1085) were compared with those of propranolol. OPC-1085 had a potency about 3 times greater than that of propranolol in blocking the positive inotropic and chronotropic effects of isoprenaline on the isolated guinea pig atrium. At a concentration of over 3 X 10(-5) M OPC-1085 produced negative inotropic and chronotropic effects. However, these effects were about 10 times weaker than those of propranolol. Suppressing effects on the rate of rise and on the maximum driving frequency of action potentials were also more than 10 times less than those of propranolol. There was almost no change in the action potential of vagus nerve after a 10 min treatment with OPC-1085 (10(-5) M), while the action potential was reduced to 60-70% with propranolol (10(-5) M).
|
A comparative study of the effects of OPC-1085 and propranolol on isolated guinea pig atrium. Effects of a newly synthesized beta-blocker, 5-(3-tert-butylamino-2-hydroxy) propoxy-3,4-dihydrocarbostyril (OPC-1085) were compared with those of propranolol. OPC-1085 had a potency about 3 times greater than that of propranolol in blocking the positive inotropic and chronotropic effects of isoprenaline on the isolated guinea pig atrium. At a concentration of over 3 X 10(-5) M OPC-1085 produced negative inotropic and chronotropic effects. However, these effects were about 10 times weaker than those of propranolol. Suppressing effects on the rate of rise and on the maximum driving frequency of action potentials were also more than 10 times less than those of propranolol. There was almost no change in the action potential of vagus nerve after a 10 min treatment with OPC-1085 (10(-5) M), while the action potential was reduced to 60-70% with propranolol (10(-5) M).
|
[
-0.05713511258363724,
-0.052224356681108475,
-0.026965372264385223,
0.01714392937719822,
0.04286341741681099,
0.00470213545486331,
0.006000697612762451,
0.03327511250972748,
-0.052942439913749695,
-0.033984892070293427,
0.0336683914065361,
0.0035411370918154716,
-0.0054211863316595554,
0.021339204162359238,
-0.03674016520380974,
-0.032750263810157776,
0.04382922127842903,
-0.06536343693733215,
-0.06214706972241402,
0.01706984080374241,
-0.03915341570973396,
-0.03952902927994728,
0.00719015346840024,
0.005988340359181166,
0.0053470320999622345,
0.023864470422267914,
0.014274418354034424,
0.03544573858380318,
-0.028997624292969704,
-0.19141125679016113,
-0.010140052065253258,
0.02111407183110714,
0.023623071610927582,
-0.010916577652096748,
-0.041188161820173264,
0.009197353385388851,
-0.0060537452809512615,
0.048399198800325394,
-0.04069746658205986,
0.09783776104450226,
0.02527071163058281,
-0.07457294315099716,
0.019931921735405922,
0.05429265648126602,
0.007159343454986811,
-0.006795462686568499,
-0.016021503135561943,
-0.027581963688135147,
0.10388462990522385,
-0.007922843098640442,
0.009321852587163448,
-0.007246131077408791,
-0.04861782118678093,
0.035192497074604034,
0.003150463569909334,
-0.05293973907828331,
-0.03025359846651554,
0.06121693551540375,
0.037055570632219315,
0.0426810123026371,
0.00433169212192297,
0.04369335249066353,
-0.17037087678909302,
0.037873946130275726,
0.041705965995788574,
0.02337559685111046,
0.02221406064927578,
0.018335185945034027,
0.04667659476399422,
0.08919096738100052,
-0.02995796874165535,
0.02311064302921295,
0.017956381663680077,
0.0049151708371937275,
-0.01293006632477045,
0.012862714938819408,
-0.017369721084833145,
0.005312724504619837,
0.0012955417623743415,
0.04596017301082611,
0.024172073230147362,
-0.008874552324414253,
-0.04724496230483055,
-0.0892028957605362,
0.0629458799958229,
-0.04216131195425987,
0.017904337495565414,
-0.015282832086086273,
-0.009913475252687931,
-0.012031099759042263,
-0.031832799315452576,
-0.046210333704948425,
-0.01964704506099224,
-0.02799602411687374,
-0.05060892179608345,
0.014684658497571945,
-0.005394574720412493,
-0.049812231212854385,
-0.025687463581562042,
0.3596752882003784,
0.033722806721925735,
0.06461373716592789,
-0.07072553783655167,
-0.03275986760854721,
0.06279942393302917,
-0.005369634833186865,
0.034517161548137665,
0.016060449182987213,
-0.015310215763747692,
0.06954215466976166,
0.05168658494949341,
0.01224640291184187,
0.043070778250694275,
-0.0033146971836686134,
-0.028266526758670807,
-0.03191050887107849,
0.06561897695064545,
-0.055207397788763046,
0.024778958410024643,
0.026228230446577072,
-0.04306922107934952,
-0.03622894361615181,
0.03946961462497711,
0.002864702371880412,
0.04211631417274475,
-0.008870154619216919,
0.004824688658118248,
0.06501837074756622,
0.03787867724895477,
0.03223837539553642,
0.03374704346060753,
0.0484270416200161,
-0.029594343155622482,
0.026306675747036934,
0.01437756884843111,
-0.029023339971899986,
-0.004769518971443176,
0.0024335107300430536,
0.04141908511519432,
-0.05480832979083061,
-0.0016697883838787675,
-0.017525341361761093,
0.03602420538663864,
-0.05154423415660858,
-0.07838305085897446,
0.09848259389400482,
0.00510011799633503,
-0.01740388758480549,
-0.031615957617759705,
0.033029232174158096,
-0.044925153255462646,
0.02048221416771412,
0.016180159524083138,
0.07201848924160004,
-0.06223583593964577,
0.024882299825549126,
-0.009363255463540554,
0.04809030145406723,
-0.07065340131521225,
-0.01710893213748932,
-0.080294668674469,
-0.0715222954750061,
-0.017863906919956207,
0.05669568106532097,
-0.05045165866613388,
-0.00810306891798973,
-0.022387858480215073,
0.02902529574930668,
-0.00534624420106411,
0.01356861274689436,
0.001265611150301993,
-0.033776938915252686,
0.024042394012212753,
-0.00665292888879776,
-0.05116112157702446,
-0.001318854745477438,
0.07789332419633865,
-0.02976257912814617,
0.0708911120891571,
-0.04534270986914635,
-0.006458595395088196,
0.020338870584964752,
0.04957233741879463,
-0.025262825191020966,
-0.012778522446751595,
0.07711343467235565,
-0.06348464637994766,
-0.04528350383043289,
-0.008329713717103004,
0.058456528931856155,
-0.025044240057468414,
-0.04001134634017944,
-0.0970667377114296,
0.018645748496055603,
-0.04851909726858139,
-0.012948055751621723,
-0.0171272661536932,
0.006324735004454851,
-0.028689896687865257,
-0.010516699403524399,
0.07287386804819107,
-0.005010074004530907,
-0.0057169776409864426,
0.031134186312556267,
0.03734087571501732,
0.033622827380895615,
-0.01006592158228159,
-0.024596640840172768,
0.059283919632434845,
-0.006795764900743961,
-0.03351486846804619,
0.03312161564826965,
0.11205184459686279,
0.03441188856959343,
-0.08456531912088394,
0.07493964582681656,
-0.04237345978617668,
0.013038741424679756,
0.06172532960772514,
0.01526432391256094,
0.0046339016407728195,
-0.01318886037915945,
0.0018039410933852196,
-0.32650646567344666,
-0.05042698606848717,
-0.03257507458329201,
-0.026217179372906685,
0.02254052460193634,
-0.03238917887210846,
0.022573834285140038,
-0.05834347382187843,
-0.04746805131435394,
-0.06576155126094818,
0.017075011506676674,
0.0801273062825203,
-0.004685699939727783,
0.020031191408634186,
0.004348034504801035,
0.08349259197711945,
0.046558674424886703,
-0.019788669422268867,
-0.001349127502180636,
0.007919097319245338,
-0.008696487173438072,
-0.0009120211470872164,
-0.03959154337644577,
0.02383996546268463,
0.005633502267301083,
0.019575361162424088,
0.08506210148334503,
-0.01683196797966957,
-0.0312521867454052,
0.034766048192977905,
0.026587067171931267,
0.007881036028265953,
0.02164030633866787,
-0.10280139744281769,
-0.006968531757593155,
-0.023997925221920013,
0.051865674555301666,
-0.018167410045862198,
0.007471010088920593,
0.03042634017765522,
-0.06466856598854065,
0.004737067501991987,
0.03192013129591942,
-0.028539400547742844,
-0.07850130647420883,
0.03288230672478676,
0.00009093575499719009,
0.05891380086541176,
-0.04462601989507675,
0.019520748406648636,
0.018184518441557884,
0.03076145425438881,
-0.01624516025185585,
0.01422679703682661,
0.04244132712483406,
-0.014535185880959034,
-0.07323338091373444,
-0.008715320378541946,
-0.03693209961056709,
-0.02416417747735977,
0.04471719637513161,
0.012993906624615192,
0.0008993357769213617,
0.021605996415019035,
-0.06319964677095413,
0.043704234063625336,
0.0510115921497345,
-0.026298798620700836,
0.022621413692831993,
-0.012221873737871647,
0.0011738918256014585,
0.08064296096563339,
-0.04269866645336151,
-0.03213009983301163,
0.09874101728200912,
-0.019441848620772362,
0.006488953251391649,
-0.01441753190010786,
-0.05999346449971199,
0.012139289639890194,
-0.0036897589452564716,
0.0007982603856362402,
0.008397919125854969,
0.021223202347755432,
-0.01944730244576931,
-0.05973467230796814,
-0.00239968067035079,
0.008585418574512005,
0.02163349837064743,
0.0008134938543662429,
-0.006005996372550726,
0.012600804679095745,
-0.02017083391547203,
-0.04233013466000557,
0.02973347343504429,
0.021347898989915848,
-0.25809091329574585,
-0.022244643419981003,
0.06949007511138916,
0.0032516878563910723,
-0.023626303300261497,
-0.01354961097240448,
0.08162510395050049,
-0.04509163275361061,
-0.057785991579294205,
0.0017448384314775467,
-0.04943615943193436,
0.038423631340265274,
0.05516113340854645,
0.028225857764482498,
-0.06564468890428543,
0.015320180915296078,
0.05787194147706032,
-0.055682726204395294,
0.015622259117662907,
-0.029352525249123573,
-0.028039416298270226,
0.04166334494948387,
0.12486982345581055,
0.004772086627781391,
-0.07286994904279709,
0.06153344362974167,
0.06736293435096741,
0.03519299626350403,
-0.02407613769173622,
0.02441294491291046,
0.009906615130603313,
0.02390064299106598,
0.08200740814208984,
-0.057519689202308655,
0.0344429686665535,
0.0017478261142969131,
-0.04643339291214943,
-0.0004036015016026795,
0.00931975245475769,
0.010731243528425694,
0.007236552890390158,
0.022091710940003395,
-0.08003063499927521,
-0.02087193913757801,
0.07282578200101852,
-0.07588943094015121,
-0.026813440024852753,
0.017524167895317078,
0.018957488238811493,
0.012150452472269535,
-0.03113549016416073,
-0.03355574235320091,
0.05916210263967514,
0.05550587177276611,
-0.021955963224172592,
-0.012246510945260525,
-0.03578709438443184,
-0.0011718120658770204,
0.00647873617708683,
-0.011382625438272953,
-0.003171192714944482,
-0.047131266444921494,
-0.02429017797112465,
0.08801885694265366,
0.08247837424278259
] |
PMID:18630
|
Stimulative effects of chelating agents, 2,2'-bipyridine and 1,10-phenanthroline, on lipid peroxidation in rat liver microsomes.
|
Well known lipid peroxidation inhibitors, 1,10-phenanthroline and 2,2'-bipyridine, stimulated microsomal NADPH- and ascorbic acid-dependent lipid peroxidation when low concentrations of these chelating agents were added to incubation mixture. The stimulatory effects of the chelating agents on lipid peroxidation were enhanced when ferrous ion was added together with the chelating agents to the mixture at a molar ratio of 1:1. Ethylenediaminetetraacetic acid (EDTA) had no stimulatory effect on lipid peroxidation. Ferrous ion-EDTA complex increased lipid peroxidation by only 20-30%, which was lower than that obtained by addition of the same concentration of ferrous ion alone. On the other hand, manganese and calcium ions, which are also inhibitors of lipid peroxidation, had no ability to stimulate lipid peroxidation even in the presence of extra ferrous ions. Changes in the lipid peroxidation by chelating agents affected the apparent activity of ethylmorphine N-demethylation.
|
Stimulative effects of chelating agents, 2,2'-bipyridine and 1,10-phenanthroline, on lipid peroxidation in rat liver microsomes. Well known lipid peroxidation inhibitors, 1,10-phenanthroline and 2,2'-bipyridine, stimulated microsomal NADPH- and ascorbic acid-dependent lipid peroxidation when low concentrations of these chelating agents were added to incubation mixture. The stimulatory effects of the chelating agents on lipid peroxidation were enhanced when ferrous ion was added together with the chelating agents to the mixture at a molar ratio of 1:1. Ethylenediaminetetraacetic acid (EDTA) had no stimulatory effect on lipid peroxidation. Ferrous ion-EDTA complex increased lipid peroxidation by only 20-30%, which was lower than that obtained by addition of the same concentration of ferrous ion alone. On the other hand, manganese and calcium ions, which are also inhibitors of lipid peroxidation, had no ability to stimulate lipid peroxidation even in the presence of extra ferrous ions. Changes in the lipid peroxidation by chelating agents affected the apparent activity of ethylmorphine N-demethylation.
|
[
-0.02881580777466297,
0.02664390578866005,
0.003926650620996952,
0.04887507110834122,
-0.007221132516860962,
-0.06348124891519547,
0.043716296553611755,
0.06595662236213684,
-0.06908700615167618,
-0.06732940673828125,
0.037061795592308044,
-0.00543547235429287,
-0.004628224764019251,
0.028186462819576263,
-0.0158404354006052,
0.003965194337069988,
-0.005678132642060518,
-0.020394569262862206,
-0.0767105296254158,
-0.009219687432050705,
-0.0472770519554615,
0.00417028833180666,
0.030136514455080032,
-0.016213996335864067,
0.003346008015796542,
0.057994816452264786,
-0.06436726450920105,
-0.0016878492897376418,
-0.06280051171779633,
-0.18635942041873932,
0.03127593919634819,
0.001366318785585463,
-0.0024304199032485485,
-0.033674456179142,
-0.01918882504105568,
0.05574930086731911,
0.024374252185225487,
0.015132210217416286,
-0.05273700878024101,
0.029974231496453285,
0.012739889323711395,
0.03817762807011604,
0.01929832436144352,
0.04677184671163559,
-0.02435128204524517,
0.021440604701638222,
0.02647130750119686,
0.0026854693423956633,
0.08739010989665985,
-0.0037873336113989353,
0.062729112803936,
0.01865861564874649,
0.03284168615937233,
0.036170896142721176,
0.02190086431801319,
-0.01690133847296238,
0.022612588480114937,
0.07921633124351501,
-0.020238004624843597,
-0.016426701098680496,
-0.0183261726051569,
0.025850625708699226,
-0.1499137282371521,
0.05342406779527664,
0.0675358697772026,
-0.0012179187033325434,
-0.030436763539910316,
0.010316484607756138,
0.06347236782312393,
0.050362374633550644,
-0.04533785209059715,
0.04596181958913803,
-0.029150594025850296,
-0.024727243930101395,
0.027494588866829872,
-0.04687582328915596,
-0.03439677506685257,
-0.0625716894865036,
-0.0071797119453549385,
-0.003709810320287943,
0.002601340413093567,
-0.01829867996275425,
-0.01224864087998867,
-0.019742103293538094,
0.03244435787200928,
0.014553011395037174,
-0.01816493831574917,
-0.03160439804196358,
-0.021758539602160454,
-0.045321907848119736,
0.013435427099466324,
-0.015040973201394081,
-0.03595900163054466,
-0.04437762126326561,
-0.07786616683006287,
0.010652406141161919,
0.015555876307189465,
-0.012072659097611904,
-0.029622379690408707,
0.3673020005226135,
0.007366930600255728,
0.02824990823864937,
-0.09910948574542999,
-0.0434638112783432,
-0.00038315143319778144,
-0.044427648186683655,
-0.020554592832922935,
-0.08952802419662476,
0.02006480097770691,
0.013885188847780228,
0.07064075022935867,
0.041018467396497726,
0.020539535209536552,
-0.047853440046310425,
-0.06860227882862091,
0.02781405672430992,
0.04672488570213318,
0.03748767450451851,
-0.030432578176259995,
0.03448906168341637,
-0.027775514870882034,
-0.048246830701828,
0.05209768936038017,
0.07600318640470505,
0.02942807972431183,
-0.020893044769763947,
0.004135257564485073,
0.09811732918024063,
0.022096557542681694,
0.045995552092790604,
0.06417511403560638,
0.02078644186258316,
0.006399289704859257,
0.03235168009996414,
-0.07906053960323334,
0.010500887408852577,
0.03337058797478676,
-0.06701137870550156,
0.04838842898607254,
0.008479890413582325,
-0.03037729114294052,
-0.035921066999435425,
-0.07074479013681412,
-0.0868133008480072,
-0.04227243363857269,
0.09033593535423279,
0.058802779763936996,
0.03941014036536217,
-0.04539244621992111,
-0.03395630791783333,
-0.0019886940717697144,
0.025810686871409416,
0.023082111030817032,
0.07789576798677444,
-0.0360264852643013,
-0.004037727601826191,
0.031122250482439995,
-0.007512530777603388,
-0.00794018805027008,
-0.04752150923013687,
-0.09392186254262924,
-0.026291705667972565,
-0.010443157516419888,
0.08856332302093506,
0.028255520388484,
-0.0041884188540279865,
-0.037210073322057724,
0.009882831014692783,
-0.014055092819035053,
-0.047484833747148514,
-0.00011553545482456684,
-0.020448816940188408,
-0.03210177645087242,
-0.0539422370493412,
-0.005992369260638952,
0.049036890268325806,
0.04052545130252838,
0.046052977442741394,
0.03877381235361099,
-0.012379206717014313,
0.09715104848146439,
0.015193222090601921,
-0.014539537951350212,
-0.006851827260106802,
-0.05778941139578819,
-0.017160627990961075,
-0.07355303317308426,
0.028868023306131363,
0.0050959656946361065,
0.025756992399692535,
-0.05300317332148552,
-0.01521792821586132,
-0.0642021968960762,
0.04716656729578972,
-0.05231351777911186,
-0.004335281439125538,
-0.0363275445997715,
-0.03896314650774002,
-0.0340411439538002,
-0.06603560596704483,
0.12419100850820541,
0.014033791609108448,
-0.00892031379044056,
0.0355725958943367,
0.031033217906951904,
-0.024158017709851265,
0.008053427562117577,
0.02771122194826603,
0.05299733206629753,
-0.032538238912820816,
-0.020128119736909866,
0.05319773405790329,
0.023180579766631126,
0.0035191511269658804,
-0.028777088969945908,
0.030191617086529732,
-0.012295515276491642,
0.02587583102285862,
0.08241035789251328,
0.02797161228954792,
0.05948574095964432,
-0.02950729802250862,
-0.02694377861917019,
-0.2914904057979584,
-0.024065831676125526,
0.0248901154845953,
0.004423229023814201,
0.02082620933651924,
-0.0701279416680336,
0.01806369423866272,
0.024714114144444466,
-0.02297172322869301,
-0.04276429861783981,
0.014505503699183464,
0.08098501712083817,
-0.026830870658159256,
-0.008191980421543121,
0.016373924911022186,
-0.03021390549838543,
0.02658790722489357,
-0.0507795549929142,
0.04785674437880516,
0.02834170125424862,
0.03207080811262131,
-0.019793475046753883,
-0.009667241014540195,
-0.015398368239402771,
0.13125845789909363,
-0.02276431769132614,
0.08550038188695908,
0.026745077222585678,
-0.05381449684500694,
-0.018011722713708878,
-0.04345293715596199,
0.055854491889476776,
-0.02202547900378704,
-0.10087478160858154,
0.0026468602009117603,
-0.021811287850141525,
0.0267946720123291,
-0.032818231731653214,
-0.00121042609680444,
-0.048332612961530685,
-0.0018917765701189637,
-0.0031773217488080263,
0.01712033897638321,
-0.06602904945611954,
-0.06020556017756462,
-0.008280673064291477,
-0.002945567248389125,
-0.0026542022824287415,
0.00224876357242465,
-0.004345277324318886,
0.02929583750665188,
0.06580737978219986,
-0.018368178978562355,
0.00897721666842699,
0.06312690675258636,
-0.021429747343063354,
-0.04090593382716179,
0.06711695343255997,
-0.039670731872320175,
-0.06583723425865173,
-0.0037069579120725393,
-0.03316375985741615,
0.06358066201210022,
-0.04152067378163338,
-0.05043415352702141,
-0.01764238066971302,
0.02878774330019951,
-0.04346194490790367,
0.03396238014101982,
0.028944512829184532,
0.037632763385772705,
0.1384698748588562,
0.006492444314062595,
-0.003187764436006546,
0.025808952748775482,
0.042086124420166016,
-0.012187155894935131,
0.002229020232334733,
-0.02185796946287155,
0.03774360194802284,
-0.09053033590316772,
0.016634171828627586,
0.05265383794903755,
-0.000596680911257863,
-0.036583077162504196,
-0.023163985460996628,
0.003205080283805728,
-0.010138938203454018,
0.0569588840007782,
0.05258157104253769,
0.030220063403248787,
0.024043947458267212,
-0.06438551098108292,
0.0027323311660438776,
0.03447215259075165,
0.050070617347955704,
-0.23638935387134552,
0.01269612554460764,
0.008805028162896633,
-0.017321554943919182,
0.010513253509998322,
-0.013819263316690922,
-0.009539881721138954,
-0.0032976665534079075,
-0.06257712095975876,
0.029727282002568245,
0.00489413645118475,
0.03696126118302345,
0.09683431684970856,
0.03662614896893501,
-0.05586947500705719,
-0.014263461343944073,
0.04713613539934158,
0.01263591181486845,
-0.002476692898198962,
0.0019529565470293164,
0.007049982436001301,
-0.02080131135880947,
0.16122619807720184,
-0.011456368491053581,
-0.009539674036204815,
0.02821301855146885,
-0.01393259596079588,
0.022063303738832474,
-0.010521025396883488,
0.0035001628566533327,
0.01642032153904438,
-0.01690986193716526,
0.06691116839647293,
-0.009822753258049488,
0.000042937066609738395,
0.05246230959892273,
-0.041768014430999756,
0.004283573478460312,
-0.11195078492164612,
0.01080286130309105,
-0.048817843198776245,
0.002298981649801135,
-0.04901881143450737,
-0.05529920011758804,
0.06539548933506012,
-0.057832926511764526,
-0.021101200953125954,
-0.031718213111162186,
-0.003644045442342758,
0.009947463870048523,
-0.00987557414919138,
-0.017655303701758385,
0.02601071074604988,
0.036661773920059204,
0.021835533902049065,
-0.035254135727882385,
-0.0023634925018996,
0.020911727100610733,
0.04287678748369217,
0.022492608055472374,
-0.007819589227437973,
0.01723325438797474,
-0.046358782798051834,
0.056368060410022736,
0.06174968555569649
] |
PMID:18631
|
Analysis of hypotensive mechanisms of pindolol, a beta-adrenoceptor blocking drugs in rats.
|
The hypotensive mechanisms of pindolol in anesthetized and conscious rats were investigated. Pindolol caused a fall in blood pressure in anesthetized, conscious and spinal rats, though in conscious rats a higher dose of the drug was required to produce such a response. This hypotension with pindolol in anesthetized and conscious rats was markedly inhibited by pretreatment with the drug itself or other beta-adrenoceptor blocking drugs. A similar phenomenon also occurred when isoproterenol was injected intravenously in anesthetized and conscious rats. The relationship between the hypotensive actions of four beta-adrenoceptor blocking drugs in anesthetized rats and their intrinsic beta-sympathomimetic actions in isolated catecholamine-depleted tracheal preparations was determined. Order of hypotensive potencies was the same as that of their intrinsic beta-sympathomimetic action, namely, pindolol greater than carteolol greater than bufetolol in equilibrium propranolol (p less than 0.05). These results suggest that the hypotension with pindolol is mediated through a decrease in the peripheral vascular resistance due to an intrinsic beta-sympathomimetic action of the drug.
|
Analysis of hypotensive mechanisms of pindolol, a beta-adrenoceptor blocking drugs in rats. The hypotensive mechanisms of pindolol in anesthetized and conscious rats were investigated. Pindolol caused a fall in blood pressure in anesthetized, conscious and spinal rats, though in conscious rats a higher dose of the drug was required to produce such a response. This hypotension with pindolol in anesthetized and conscious rats was markedly inhibited by pretreatment with the drug itself or other beta-adrenoceptor blocking drugs. A similar phenomenon also occurred when isoproterenol was injected intravenously in anesthetized and conscious rats. The relationship between the hypotensive actions of four beta-adrenoceptor blocking drugs in anesthetized rats and their intrinsic beta-sympathomimetic actions in isolated catecholamine-depleted tracheal preparations was determined. Order of hypotensive potencies was the same as that of their intrinsic beta-sympathomimetic action, namely, pindolol greater than carteolol greater than bufetolol in equilibrium propranolol (p less than 0.05). These results suggest that the hypotension with pindolol is mediated through a decrease in the peripheral vascular resistance due to an intrinsic beta-sympathomimetic action of the drug.
|
[
-0.04753018543124199,
0.013439461588859558,
-0.009799533523619175,
-0.006502218544483185,
0.0020378418266773224,
0.0017806021496653557,
0.04005501791834831,
0.029278073459863663,
-0.028781449422240257,
-0.0364689938724041,
-0.009091799147427082,
-0.005586443934589624,
-0.009773912839591503,
0.06478358060121536,
-0.05376582220196724,
-0.0007515883771702647,
0.041691869497299194,
-0.006717781536281109,
-0.05050043389201164,
-0.03610298037528992,
0.01167736854404211,
0.007481337059289217,
0.004102805629372597,
0.01441981177777052,
-0.015840791165828705,
0.00858133751899004,
-0.0005046516889706254,
0.015859676524996758,
-0.07241092622280121,
-0.18817727267742157,
0.04407238960266113,
-0.002284975489601493,
0.03079340048134327,
-0.0375557579100132,
0.029518762603402138,
0.01079528871923685,
-0.014877072535455227,
0.0839431881904602,
0.024196090176701546,
0.025138895958662033,
0.027487725019454956,
0.0008899722597561777,
0.021996159106492996,
0.016891488805413246,
0.014229864813387394,
-0.024381693452596664,
-0.015488501638174057,
-0.00856794510036707,
0.10679358243942261,
-0.011212402954697609,
0.015248407609760761,
-0.0004061750369146466,
0.009731215424835682,
0.041720692068338394,
-0.009469647891819477,
-0.04840351641178131,
-0.03676425293087959,
0.025916393846273422,
0.00862575601786375,
0.055650558322668076,
-0.003909430932253599,
0.04397714510560036,
-0.16350057721138,
0.07475420832633972,
0.04372045397758484,
0.07223456352949142,
-0.024320414289832115,
0.06474176049232483,
0.041814737021923065,
0.04111277312040329,
0.045307137072086334,
0.019786469638347626,
-0.021844660863280296,
-0.05156476050615311,
-0.041494496166706085,
-0.023939039558172226,
0.018047062680125237,
-0.02980385534465313,
-0.014061364345252514,
-0.005507715977728367,
0.08067390322685242,
0.006180434487760067,
-0.03119521401822567,
-0.0032930870074778795,
0.007641046307981014,
-0.05685613676905632,
0.08650888502597809,
-0.0432572215795517,
-0.04468173533678055,
-0.048136088997125626,
-0.0795232504606247,
-0.06112842261791229,
0.011110158637166023,
0.01163326296955347,
-0.008981729857623577,
0.019359352067112923,
-0.04192434623837471,
-0.04546566680073738,
-0.03438678756356239,
0.35021576285362244,
0.010711108334362507,
0.040920305997133255,
-0.0472446009516716,
-0.020350590348243713,
0.04027717188000679,
-0.030853528529405594,
-0.014838439412415028,
-0.011459189467132092,
-0.03241703659296036,
0.08598592132329941,
0.0883038341999054,
-0.017505617812275887,
0.07342404127120972,
-0.005459953099489212,
-0.015759866684675217,
-0.06709988415241241,
0.01856204681098461,
0.0060861436650156975,
0.005236413329839706,
0.0019964580424129963,
-0.011386744678020477,
0.015389245003461838,
0.03699096664786339,
0.046276919543743134,
0.08834322541952133,
-0.04355696588754654,
-0.018520811572670937,
0.08507347106933594,
0.08258041739463806,
0.019808314740657806,
0.08224162459373474,
0.017385033890604973,
-0.020011182874441147,
0.02047278732061386,
0.027103938162326813,
-0.023321885615587234,
0.021282533183693886,
-0.03615911304950714,
0.03569291532039642,
-0.047841791063547134,
0.006575044710189104,
-0.0351249985396862,
0.04308028519153595,
-0.08564368635416031,
-0.06943506002426147,
0.09922275692224503,
0.04841282591223717,
-0.001989393262192607,
-0.0632113441824913,
0.06491580605506897,
-0.07073047012090683,
0.004129368811845779,
-0.0012923767790198326,
0.054389484226703644,
-0.06613883376121521,
0.010949292220175266,
-0.006531308870762587,
0.026334403082728386,
-0.0169992558658123,
-0.0088523393496871,
-0.043502021580934525,
-0.0367843396961689,
-0.03163255751132965,
0.028827236965298653,
-0.005213412456214428,
0.015599541366100311,
-0.06562144309282303,
0.005603133235126734,
-0.021648699417710304,
0.03652628883719444,
-0.022586315870285034,
0.03150003030896187,
0.007794479373842478,
-0.06812134385108948,
-0.07321111112833023,
0.007622066419571638,
0.047519855201244354,
-0.019552091136574745,
0.03380169719457626,
-0.06745272874832153,
0.021845556795597076,
-0.01760859042406082,
0.02553972229361534,
0.029121242463588715,
0.03420839086174965,
0.040356311947107315,
-0.05503667891025543,
0.030806638300418854,
-0.021735602989792824,
0.06640216708183289,
-0.015846310183405876,
-0.0156902763992548,
-0.08858954161405563,
-0.012433487921953201,
-0.025031261146068573,
-0.02834416553378105,
0.010279810056090355,
0.020287295803427696,
-0.05283389240503311,
0.017716802656650543,
0.061342038214206696,
0.003830431494861841,
-0.0031270089093595743,
0.05178307369351387,
-0.014933253638446331,
0.08598202466964722,
-0.028398483991622925,
0.01882007159292698,
0.08377867192029953,
-0.0038963903207331896,
-0.009510193951427937,
0.031759437173604965,
0.006642486434429884,
0.011591922491788864,
-0.008678678423166275,
0.04607834666967392,
-0.009751572273671627,
0.03700467571616173,
0.031407106667757034,
0.054958559572696686,
0.044839248061180115,
-0.051893576979637146,
0.07172518223524094,
-0.3111956715583801,
-0.06544654071331024,
-0.04932554438710213,
-0.06034458056092262,
0.01874219998717308,
-0.03382544219493866,
0.08447490632534027,
-0.07650791853666306,
-0.059557851403951645,
-0.026039890944957733,
0.02939404360949993,
0.0038685461040586233,
-0.01421709917485714,
0.017206192016601562,
-0.029138660058379173,
0.08816789090633392,
0.04700763151049614,
-0.06391613185405731,
0.002050398150458932,
-0.009292270988225937,
0.004268105607479811,
-0.03642662614583969,
-0.015850098803639412,
0.016145819798111916,
0.004581272602081299,
0.00174420396797359,
0.06555657833814621,
-0.01095249131321907,
-0.03983578085899353,
-0.020264752209186554,
-0.006612956989556551,
0.0393180213868618,
0.007637702394276857,
-0.11163423955440521,
0.042033690959215164,
-0.05440528690814972,
0.03554835543036461,
0.004640377592295408,
0.05075397342443466,
0.011787286028265953,
-0.050688184797763824,
0.037176359444856644,
-0.0013297375990077853,
0.004475072026252747,
-0.05083596333861351,
0.0526634082198143,
0.011520239524543285,
0.04134582728147507,
-0.07313313335180283,
0.0351148396730423,
0.0031029670499265194,
0.003945204429328442,
0.009346316568553448,
0.003111298196017742,
0.05405303090810776,
-0.024456175044178963,
-0.048595547676086426,
-0.037695031613111496,
-0.03668975457549095,
-0.018605699762701988,
0.035397786647081375,
0.0032091792672872543,
0.0005115233943797648,
-0.03229691833257675,
-0.033813923597335815,
0.014203112572431564,
0.029086071997880936,
-0.005172614939510822,
0.03645604848861694,
0.04537927359342575,
-0.006453508976846933,
0.1281498521566391,
0.0036362919490784407,
-0.009567130357027054,
0.029409795999526978,
0.010847596451640129,
-0.045364268124103546,
-0.06435900926589966,
-0.043038494884967804,
0.0025607889983803034,
0.011430084705352783,
-0.065497487783432,
0.0055900681763887405,
0.026285359635949135,
-0.06471779942512512,
-0.05153603106737137,
0.04612966999411583,
0.011670764535665512,
0.085887610912323,
-0.02339540421962738,
-0.06099884957075119,
0.02090630866587162,
0.005745850503444672,
-0.014819684438407421,
0.05624667555093765,
0.025230636820197105,
-0.2728863060474396,
0.0016029676189646125,
0.061268314719200134,
-0.019792117178440094,
0.028804808855056763,
-0.008975732140243053,
0.05550381913781166,
-0.046202097088098526,
-0.02505490742623806,
0.00637560710310936,
-0.03012603148818016,
0.032834652811288834,
0.05114902928471565,
0.07451383024454117,
-0.014413399621844292,
-0.0211372971534729,
0.035229410976171494,
-0.005546549800783396,
0.0759723111987114,
-0.03752564638853073,
-0.021391239017248154,
0.005823494866490364,
0.12843231856822968,
0.006445277016609907,
-0.04142650216817856,
0.04534124583005905,
0.009319634176790714,
-0.0026232192758470774,
-0.018348701298236847,
0.028604689985513687,
-0.04961124807596207,
-0.020598292350769043,
0.04465791955590248,
-0.04647379368543625,
0.025126593187451363,
0.01599305123090744,
-0.07603367418050766,
-0.003309918800368905,
0.04175110533833504,
-0.02654312551021576,
-0.03033454716205597,
0.009007085114717484,
-0.07765394449234009,
-0.017291421070694923,
0.0804685428738594,
-0.08800619095563889,
-0.07648127526044846,
-0.008935049176216125,
0.011672265827655792,
0.04926597699522972,
-0.04326386749744415,
-0.0360071174800396,
0.027530696243047714,
0.04219210892915726,
0.0037240940146148205,
-0.028598252683877945,
-0.015593321993947029,
-0.011199415661394596,
0.008969757705926895,
-0.018459497019648552,
-0.0014521775301545858,
-0.03226175904273987,
-0.050838980823755264,
0.04452616348862648,
0.04468926042318344
] |
PMID:18635
|
Drug-induced dystonic reactions.
|
In the 12 unusual cases of drug-induced dystonic reaction discussed, major tranquilizers were ingested unaccompanied by anti-parkinsonian drugs. The five characteristics of the drug-induced dystonic reactions are oculogyric crisis, torticollic crisis, buccolingual crisis, opisthotonic crisis, and tortipelvic and gait crisis. Neuromuscular manifestations occur in the oculogyric, torticollic, buccolingual, opisthotonic and tortipelvic regions. The basic treatment is to discontinue major tranquilizers and administer anticholinergic agents to block the enhanced cholinergic function. Useful anticholinergic and antihistaminic agents for the treatment of drug-induced dystonic reactions are listed to aid in the selection of appropriate therapeutic remedies.
|
Drug-induced dystonic reactions. In the 12 unusual cases of drug-induced dystonic reaction discussed, major tranquilizers were ingested unaccompanied by anti-parkinsonian drugs. The five characteristics of the drug-induced dystonic reactions are oculogyric crisis, torticollic crisis, buccolingual crisis, opisthotonic crisis, and tortipelvic and gait crisis. Neuromuscular manifestations occur in the oculogyric, torticollic, buccolingual, opisthotonic and tortipelvic regions. The basic treatment is to discontinue major tranquilizers and administer anticholinergic agents to block the enhanced cholinergic function. Useful anticholinergic and antihistaminic agents for the treatment of drug-induced dystonic reactions are listed to aid in the selection of appropriate therapeutic remedies.
|
[
-0.017246125265955925,
0.028988434001803398,
0.024826057255268097,
0.029565393924713135,
-0.008682114072144032,
0.012686535716056824,
0.0902101919054985,
0.0631319135427475,
-0.04406552016735077,
-0.07590330392122269,
0.008640876039862633,
-0.00419816467911005,
0.013057355768978596,
0.049150217324495316,
-0.0037331003695726395,
-0.00411018542945385,
0.06102100759744644,
-0.035315416753292084,
-0.03869922459125519,
0.010584132745862007,
-0.04381699860095978,
0.040431488305330276,
-0.004866709932684898,
0.054625533521175385,
0.02073134109377861,
0.03804268687963486,
-0.031786322593688965,
0.045680031180381775,
-0.06314168870449066,
-0.13328073918819427,
0.032560113817453384,
0.03490665555000305,
-0.04019494727253914,
-0.08441167324781418,
-0.004214603919535875,
0.007185878697782755,
-0.09667346626520157,
0.05505404248833656,
-0.003400254063308239,
-0.002595932688564062,
0.013597230426967144,
-0.0016157414065673947,
0.03282427787780762,
-0.011174199171364307,
-0.005749706644564867,
-0.03718729317188263,
-0.013678307645022869,
0.02978162094950676,
0.11847665160894394,
-0.017290886491537094,
0.037437502294778824,
-0.05387711897492409,
0.059702664613723755,
0.01534306164830923,
-0.03054436668753624,
-0.03571302816271782,
-0.02752823755145073,
-0.0014910052996128798,
0.0025765537284314632,
0.030751610174775124,
-0.009773454628884792,
0.04670533165335655,
-0.06572938710451126,
-0.004806355573236942,
0.042786043137311935,
0.012343333102762699,
0.04489198327064514,
-0.010203015990555286,
0.07380440086126328,
0.08603493124246597,
-0.0018665599636733532,
-0.0013447243254631758,
-0.04063727334141731,
0.048753924667835236,
-0.017807278782129288,
-0.03414894640445709,
-0.021214941516518593,
-0.0023545436561107635,
0.005200714338570833,
0.020081907510757446,
0.07586272805929184,
-0.0066344523802399635,
0.024761538952589035,
-0.0811983197927475,
0.030996225774288177,
-0.007489199750125408,
0.03473499044775963,
-0.025569722056388855,
-0.049118783324956894,
-0.016176806762814522,
-0.009686559438705444,
0.022430138662457466,
0.008162369951605797,
-0.023899121209979057,
-0.07105936110019684,
0.037286918610334396,
-0.00661192461848259,
-0.06373909115791321,
-0.038296595215797424,
0.3513476252555847,
0.010485134087502956,
-0.027998732402920723,
0.05842594429850578,
0.027207113802433014,
0.012428505346179008,
0.009759071283042431,
0.04703700914978981,
-0.04453479126095772,
-0.06586292386054993,
0.045155443251132965,
0.04605424031615257,
0.015439743176102638,
0.03570648655295372,
-0.04021814838051796,
-0.026789408177137375,
-0.019534043967723846,
0.0782475396990776,
-0.008944139815866947,
-0.03086058981716633,
0.04932546243071556,
-0.03089456632733345,
-0.010165289044380188,
0.007832554168999195,
-0.0068396409042179585,
0.028134729713201523,
0.01413754466921091,
-0.03180667385458946,
0.07447925209999084,
0.038378480821847916,
-0.0013714595697820187,
0.03768005222082138,
0.014389055781066418,
-0.0116497203707695,
0.04607819765806198,
0.0592169389128685,
-0.0477653443813324,
0.028107425197958946,
-0.011686410754919052,
0.031918738037347794,
0.003561357269063592,
-0.014996257610619068,
-0.06558265537023544,
-0.0009607052197679877,
-0.07272843271493912,
-0.07968720048666,
0.06311658769845963,
-0.03258470445871353,
-0.037752747535705566,
-0.00862808432430029,
0.07323254644870758,
-0.055117905139923096,
0.05278646945953369,
-0.058253172785043716,
0.0757548063993454,
-0.05242240056395531,
0.07038278877735138,
0.0005434785271063447,
0.005746058654040098,
0.02526722475886345,
-0.008820525370538235,
-0.0378238819539547,
-0.06628761440515518,
-0.001033478300087154,
0.15713070333003998,
-0.0025055599398911,
-0.019377272576093674,
-0.055500347167253494,
-0.004683406557887793,
-0.07208405435085297,
0.05269310250878334,
-0.00505890604108572,
0.0077036707662045956,
-0.030063653364777565,
-0.025767318904399872,
-0.018832407891750336,
-0.014895516447722912,
-0.01011422835290432,
-0.033882711082696915,
-0.031285494565963745,
0.01334602851420641,
0.053012121468782425,
0.02331393212080002,
-0.002606977242976427,
0.006022115238010883,
0.012264745309948921,
0.01383508462458849,
-0.06116171181201935,
-0.004505527205765247,
0.09569479525089264,
-0.00894927978515625,
-0.008943209424614906,
-0.06410577148199081,
-0.09767977148294449,
-0.012332098558545113,
-0.05813245475292206,
0.0057815248146653175,
-0.04907030984759331,
0.01708468236029148,
0.02996492199599743,
0.0020569267217069864,
0.07018458843231201,
0.06912855803966522,
-0.02956255152821541,
0.08687394857406616,
0.028818292543292046,
-0.008794025518000126,
-0.004213597625494003,
0.0047097112983465195,
0.04664340987801552,
0.0097664101049304,
-0.020355962216854095,
0.02631743997335434,
0.055016785860061646,
-0.008320866152644157,
-0.04103216156363487,
0.04257694631814957,
-0.057714901864528656,
0.06415075808763504,
0.06785137206315994,
-0.024471303448081017,
0.06850297003984451,
-0.007731837686151266,
-0.010920548811554909,
-0.31553366780281067,
-0.0840020403265953,
0.0008373683085665107,
-0.025393566116690636,
0.02592838555574417,
-0.005154413171112537,
-0.006909504067152739,
-0.06192423403263092,
0.013489310629665852,
-0.03991123288869858,
0.03004601038992405,
0.07616924494504929,
0.006694810464978218,
-0.1024511530995369,
0.006892743520438671,
0.009204749949276447,
0.031010976061224937,
-0.03424519672989845,
0.0260576531291008,
-0.059484876692295074,
0.01488474477082491,
-0.036441292613744736,
-0.043884437531232834,
-0.019452210515737534,
0.0003828262852039188,
-0.0472823902964592,
0.10454121232032776,
0.06134733557701111,
-0.035367451608181,
-0.035633787512779236,
-0.03173714503645897,
0.020215703174471855,
-0.009701604023575783,
-0.07056587189435959,
0.06491956859827042,
0.007368061225861311,
0.013028839603066444,
0.00566865224391222,
0.0004405622894410044,
-0.009168654680252075,
-0.02327929623425007,
0.010151308961212635,
-0.01685178279876709,
-0.00004815219290321693,
-0.030170325189828873,
0.07423990219831467,
-0.025333283469080925,
-0.0033906211610883474,
0.027580082416534424,
-0.009122883901000023,
0.03621465712785721,
0.021799270063638687,
0.021070348098874092,
0.007569871377199888,
-0.04856825992465019,
-0.009627016261219978,
-0.11713262647390366,
-0.017981745302677155,
-0.06116476655006409,
-0.07139387726783752,
0.02454315312206745,
0.047706108540296555,
0.040062591433525085,
-0.05706008896231651,
-0.020597511902451515,
0.0058833840303123,
-0.004180612042546272,
-0.009644543752074242,
0.04494123533368111,
0.023547234013676643,
0.009165500290691853,
0.15823239088058472,
-0.017319614067673683,
0.03293202072381973,
0.060667552053928375,
0.0347948856651783,
-0.03980599716305733,
0.015153615735471249,
-0.08218833059072495,
0.0021892613731324673,
-0.03173371031880379,
0.03428739681839943,
-0.012729387730360031,
0.06101943552494049,
-0.07014772295951843,
-0.06638266146183014,
-0.016363603994250298,
0.002844849368557334,
0.038005415350198746,
-0.0104864202439785,
-0.004652709700167179,
0.0024264990352094173,
-0.018216805532574654,
0.03827080875635147,
0.09064899384975433,
0.1091335117816925,
-0.28198370337486267,
0.0015912024537101388,
0.022675801068544388,
0.045842234045267105,
0.013560152612626553,
0.03502720966935158,
-0.014706036075949669,
0.01934649609029293,
-0.028302062302827835,
-0.0471298024058342,
0.027476629242300987,
-0.021801818162202835,
0.037653058767318726,
-0.020441342145204544,
-0.022306131199002266,
-0.011054294183850288,
0.0733557865023613,
0.01721593737602234,
0.019743043929338455,
-0.0019457087619230151,
0.007109463680535555,
0.03161562234163284,
0.14362309873104095,
-0.0047910865396261215,
-0.010147749446332455,
0.022474510595202446,
0.007845979183912277,
0.07135139405727386,
-0.020623592659831047,
0.000006982928425713908,
-0.016634082421660423,
-0.001987776020541787,
0.029573582112789154,
0.006863311864435673,
-0.005316861439496279,
-0.04416114464402199,
-0.07863865792751312,
0.0026430580765008926,
-0.017368141561746597,
-0.015651537105441093,
-0.04565487056970596,
-0.007012094371020794,
-0.04054723680019379,
-0.019157979637384415,
0.06597346067428589,
-0.015875915065407753,
-0.0516110360622406,
-0.04953688755631447,
-0.019333001226186752,
0.015886453911662102,
0.010795769281685352,
-0.06327353417873383,
-0.0011307287495583296,
0.01962430402636528,
0.017911076545715332,
-0.0098698940128088,
-0.004067914094775915,
0.03427274897694588,
0.027988063171505928,
0.0014469235902652144,
0.0019061423372477293,
-0.05480887368321419,
-0.001797979697585106,
-0.049386754631996155,
-0.010076078586280346
] |
PMID:18637
|
[Ocular disturbances in asthenic neurosis and their treatment (author's transl)].
|
The author has studied over 200 cases of asthenic neurosis. The ocular manifestations could be classified into four clinical types according to the dominant ocular symptoms. 58.34% of these patients were female and 41.66% were male. 78% of the patients were between 20 and 40 years. 59% were students. In 75% of these cases a raised retinal blood pressure as an ocular manifestation was discovered. The ocular disturbances in asthenic neurosis are present especially in persons who regularly work more than 8 hours continuously. The treatment consists of physical and intellectual rest periods, sedatives, tonics, and connective tissue therapy with extracts of whole eyeballs. In 80% of cases we had favourable results.
|
[Ocular disturbances in asthenic neurosis and their treatment (author's transl)]. The author has studied over 200 cases of asthenic neurosis. The ocular manifestations could be classified into four clinical types according to the dominant ocular symptoms. 58.34% of these patients were female and 41.66% were male. 78% of the patients were between 20 and 40 years. 59% were students. In 75% of these cases a raised retinal blood pressure as an ocular manifestation was discovered. The ocular disturbances in asthenic neurosis are present especially in persons who regularly work more than 8 hours continuously. The treatment consists of physical and intellectual rest periods, sedatives, tonics, and connective tissue therapy with extracts of whole eyeballs. In 80% of cases we had favourable results.
|
[
0.025255931541323662,
0.028839988633990288,
0.0028637098148465157,
0.05028527230024338,
-0.0384703129529953,
0.03511842340230942,
0.11172065138816833,
0.04877224564552307,
-0.06851776689291,
-0.01054881140589714,
0.0013440059265121818,
-0.04269188269972801,
-0.016824521124362946,
0.06916714459657669,
-0.03435269743204117,
-0.005051405634731054,
-0.020605387166142464,
-0.033534131944179535,
-0.002616230631247163,
0.06256961822509766,
-0.010326635092496872,
0.006738059688359499,
0.060206301510334015,
-0.01650221459567547,
-0.015581504441797733,
-0.030296947807073593,
-0.016116468235850334,
-0.0008034142665565014,
-0.014041325077414513,
-0.14140421152114868,
-0.023773329332470894,
0.0029593578074127436,
-0.022182684391736984,
0.020318306982517242,
0.05522312596440315,
-0.04128216952085495,
-0.08011408150196075,
0.018540918827056885,
-0.04372350126504898,
-0.004126301500946283,
-0.0014765110099688172,
-0.029091406613588333,
-0.0021452242508530617,
-0.016051748767495155,
0.026160024106502533,
0.012156170792877674,
-0.013039452955126762,
-0.033849675208330154,
0.11363525688648224,
0.014787404797971249,
-0.02314210683107376,
-0.026156963780522346,
0.014539827592670918,
0.08053671568632126,
-0.0044196657836437225,
-0.05678539723157883,
-0.023703839629888535,
0.011518219485878944,
0.026090450584888458,
0.022912688553333282,
0.02297981083393097,
0.002694475930184126,
-0.08847032487392426,
0.015754837542772293,
0.012548405677080154,
-0.004719661083072424,
-0.0028445706702768803,
-0.06883678585290909,
0.0389728844165802,
-0.037773940712213516,
-0.036026157438755035,
0.03531792759895325,
-0.0016751629300415516,
0.028537601232528687,
-0.022341061383485794,
-0.006156253628432751,
-0.008419989608228207,
-0.04950118064880371,
-0.022278379648923874,
0.03800436481833458,
0.01705077476799488,
0.01582260988652706,
-0.011103752069175243,
-0.09774515777826309,
0.05718112736940384,
0.020481716841459274,
-0.03652343526482582,
-0.04584817960858345,
-0.037630800157785416,
-0.0092396205291152,
-0.038163140416145325,
-0.004167457576841116,
-0.054559770971536636,
-0.009224331006407738,
-0.020420953631401062,
0.007167162839323282,
-0.038540489971637726,
-0.005116925109177828,
-0.04536346718668938,
0.39625269174575806,
0.03501037508249283,
-0.004796889144927263,
0.0351358987390995,
0.007991964928805828,
-0.0021344393026083708,
-0.044897809624671936,
-0.018905069679021835,
-0.04025576263666153,
0.016668209806084633,
0.04115898534655571,
-0.039829038083553314,
0.02738356962800026,
0.039222583174705505,
-0.015726888552308083,
-0.030729517340660095,
0.027974391356110573,
0.09549926221370697,
-0.04240915924310684,
-0.0016948075499385595,
0.02044549584388733,
0.01902134157717228,
0.01797153428196907,
0.02815546840429306,
0.007804595399647951,
0.03187747672200203,
0.02578754536807537,
-0.05122178792953491,
0.061675310134887695,
0.02325342409312725,
0.04228075593709946,
0.010757793672382832,
0.031192263588309288,
0.008752407506108284,
-0.02222621813416481,
0.06608781218528748,
0.006348060443997383,
0.06046103313565254,
-0.016479939222335815,
0.02103462442755699,
0.01657791994512081,
0.02206593193113804,
-0.07671032845973969,
0.024255145341157913,
-0.05839235335588455,
-0.024263521656394005,
0.07518898695707321,
-0.004490746650844812,
0.042672574520111084,
-0.029706306755542755,
0.017716756090521812,
0.0125084538012743,
0.06860946863889694,
-0.03301621228456497,
0.03004336915910244,
0.004471629858016968,
0.034835536032915115,
0.017067328095436096,
-0.0002345627872273326,
-0.05675569176673889,
0.054171912372112274,
-0.03850644454360008,
-0.04938235506415367,
-0.01786203682422638,
0.0694274976849556,
0.07343470305204391,
-0.006597642321139574,
-0.020863143727183342,
-0.03275750204920769,
-0.02261558175086975,
-0.029271787032485008,
0.00724183302372694,
-0.012624911032617092,
0.0616898387670517,
0.00824258103966713,
-0.009920473210513592,
0.02820097655057907,
-0.041629940271377563,
-0.033296823501586914,
-0.03994031995534897,
0.03162968531250954,
0.04734831675887108,
0.01918502151966095,
-0.04653323069214821,
-0.0019597525242716074,
-0.024982620030641556,
-0.005680106580257416,
-0.026212036609649658,
0.013324371539056301,
0.005786367226392031,
-0.04739954322576523,
-0.06250172108411789,
-0.023749323561787605,
-0.11116322129964828,
-0.0049368576146662235,
0.025742467492818832,
0.03374659642577171,
-0.01481346134096384,
-0.09871257841587067,
-0.011590933427214622,
-0.001326646306551993,
0.05280236527323723,
-0.016743093729019165,
0.010763025842607021,
0.023671230301260948,
0.07520730793476105,
0.07611076533794403,
-0.0368397980928421,
-0.014622321352362633,
0.017324546352028847,
-0.0013165964046493173,
-0.07316270470619202,
0.036205314099788666,
0.02695079706609249,
0.06168104708194733,
-0.02819671854376793,
0.038048721849918365,
-0.04371783509850502,
-0.014525596983730793,
0.05995628610253334,
0.032090749591588974,
0.1000019833445549,
-0.029153142124414444,
-0.02937851846218109,
-0.32885003089904785,
-0.015591771341860294,
0.007028677966445684,
-0.0621943399310112,
0.027375338599085808,
-0.04726526886224747,
0.00711061479523778,
-0.017075849696993828,
0.024915961548686028,
-0.008421757258474827,
0.02061753161251545,
0.06106638163328171,
0.004395321011543274,
-0.07330763339996338,
0.0016847539227455854,
0.025193728506565094,
0.037869952619075775,
-0.016898106783628464,
-0.010714845731854439,
-0.03303798660635948,
0.030020754784345627,
0.025197219103574753,
0.016360219568014145,
-0.013249860145151615,
0.003602305892854929,
-0.02391647733747959,
0.11240589618682861,
0.0006965632201172411,
0.017988018691539764,
-0.0712750256061554,
0.002562873996794224,
0.029427699744701385,
-0.007438771892338991,
-0.07303518056869507,
0.028012655675411224,
-0.050235189497470856,
0.017280759289860725,
0.019701918587088585,
-0.03146497160196304,
0.03997964411973953,
-0.02241690456867218,
-0.02186756767332554,
-0.01962575875222683,
0.06283068656921387,
-0.07233229279518127,
0.03589180111885071,
0.012334663420915604,
0.00036163642653264105,
-0.00005471009717439301,
-0.005136164836585522,
-0.002374630654230714,
-0.04491710662841797,
-0.03370502218604088,
0.002743207849562168,
-0.03537705913186073,
-0.031442299485206604,
-0.1139795109629631,
-0.03575323894619942,
-0.08316363394260406,
-0.002778249094262719,
0.015503433533012867,
0.005588008090853691,
0.02758057601749897,
-0.059539373964071274,
-0.0003964714123867452,
0.02725226804614067,
0.012870492413640022,
0.03427911549806595,
0.0554991215467453,
0.0360012948513031,
-0.03155103325843811,
0.100666344165802,
-0.04773777350783348,
0.005152188707143068,
0.023424182087183,
0.07571244984865189,
-0.008851907216012478,
0.0390038900077343,
-0.02994697540998459,
0.034980613738298416,
-0.015462981536984444,
-0.07266098260879517,
-0.0009710347512736917,
0.02188349887728691,
-0.04363013431429863,
-0.03651493415236473,
-0.003185670357197523,
-0.016286391764879227,
0.02628006413578987,
-0.03315739333629608,
0.02930961735546589,
0.014273656532168388,
-0.0374760627746582,
0.0016196520300582051,
0.020424656569957733,
0.0651555061340332,
-0.2918596565723419,
-0.0037044319324195385,
0.0028035491704940796,
0.03126964718103409,
-0.010364517569541931,
0.041560642421245575,
-0.03109113872051239,
-0.004261631518602371,
-0.01789712905883789,
-0.03316012769937515,
0.006204502657055855,
0.016819266602396965,
0.03670983016490936,
-0.013290648348629475,
0.0641779974102974,
-0.024977460503578186,
0.08385571837425232,
0.004854386206716299,
0.08844055235385895,
-0.04569678381085396,
0.021841246634721756,
0.011411506682634354,
0.16699695587158203,
-0.008489852771162987,
-0.04621482267975807,
0.007719441317021847,
-0.0023985926527529955,
0.05439792200922966,
-0.10429450869560242,
0.03527863696217537,
0.044510919600725174,
0.04348283261060715,
0.0739983469247818,
0.08641870319843292,
0.08328757435083389,
-0.011495874263346195,
-0.017634376883506775,
0.051912155002355576,
0.03126955032348633,
-0.01517874002456665,
0.017141958698630333,
0.0036770349834114313,
-0.037325695157051086,
-0.024213161319494247,
0.09525632858276367,
-0.03148553892970085,
-0.03518931195139885,
0.007082098629325628,
-0.0313677154481411,
0.026271484792232513,
-0.06775461137294769,
-0.056830231100320816,
0.05421840026974678,
0.08357679098844528,
-0.003605964593589306,
-0.021945858374238014,
-0.04051486775279045,
0.022601138800382614,
-0.028579767793416977,
-0.05481647700071335,
-0.027830198407173157,
0.003307856386527419,
-0.019930221140384674,
0.01836506463587284,
0.026317793875932693
] |
PMID:18638
|
Plasma catecholamines in exercise induced bronchoconstriction.
|
Plasma nor-epinephrine (NE) and epinephrine (E) levels at rest and immediately after exercise were estimated in 8 patients with asymptomatic extrinsic allergic bronchial asthma. The patients had a normal airway resistance at rest and developed a marked bronchoconstriction (EIB) during exercise, which could be prevented by previous alpha-adrenergic blockade with phentolamine. In 7 control persons NE and E levels were measured also after beta-adrenergic blockade with propranolol. The following results were obtained: 1. At rest NE levels showed no significant differences between the groups. After exercise an increase of NE was observed in all groups, but in patients, even after phentolamine, and in normals after propranolol the increase was significantly higher than in the normal group within the control test. 2. No significant differences between the groups were found in E levels at rest and after exercise. Exercise caused no significant increase of E levels, except in the normals after propranolol application. 3. No significant correlation existed between NE levels and the increase of airway resistance after exercise. It is concluded that during exercise in asthmatics the sympathetic activity is enhanced, but the provocation of an EIB does not seem to be mediated by enhanced plasma NE levels.
|
Plasma catecholamines in exercise induced bronchoconstriction. Plasma nor-epinephrine (NE) and epinephrine (E) levels at rest and immediately after exercise were estimated in 8 patients with asymptomatic extrinsic allergic bronchial asthma. The patients had a normal airway resistance at rest and developed a marked bronchoconstriction (EIB) during exercise, which could be prevented by previous alpha-adrenergic blockade with phentolamine. In 7 control persons NE and E levels were measured also after beta-adrenergic blockade with propranolol. The following results were obtained: 1. At rest NE levels showed no significant differences between the groups. After exercise an increase of NE was observed in all groups, but in patients, even after phentolamine, and in normals after propranolol the increase was significantly higher than in the normal group within the control test. 2. No significant differences between the groups were found in E levels at rest and after exercise. Exercise caused no significant increase of E levels, except in the normals after propranolol application. 3. No significant correlation existed between NE levels and the increase of airway resistance after exercise. It is concluded that during exercise in asthmatics the sympathetic activity is enhanced, but the provocation of an EIB does not seem to be mediated by enhanced plasma NE levels.
|
[
-0.03213495388627052,
0.029849186539649963,
0.012653234414756298,
0.043501630425453186,
-0.009928463958203793,
0.027212655171751976,
0.06490152329206467,
0.01289370097219944,
-0.036398228257894516,
-0.02092765085399151,
-0.044932927936315536,
-0.028872065246105194,
-0.03891649842262268,
0.04913708195090294,
0.009103599935770035,
-0.04063250496983528,
0.04501021280884743,
-0.017004145309329033,
-0.08205683529376984,
0.03090825118124485,
-0.054153069853782654,
0.028327643871307373,
0.08951668441295624,
0.030155349522829056,
0.05108392611145973,
0.048819299787282944,
-0.03659040480852127,
-0.001797004253603518,
-0.03175732493400574,
-0.16143792867660522,
0.03316717967391014,
-0.040576815605163574,
0.020067250356078148,
0.059452980756759644,
-0.023496992886066437,
0.022060375660657883,
-0.08407163619995117,
0.051175426691770554,
-0.02022078074514866,
0.07293291389942169,
-0.004214034881442785,
-0.011389114893972874,
0.04219420626759529,
0.018076209351420403,
0.04211701452732086,
0.028512079268693924,
-0.062085822224617004,
-0.0200283695012331,
0.08476679772138596,
0.0276327021420002,
0.0566742867231369,
0.013229496777057648,
0.039812538772821426,
0.049274180084466934,
0.014398515224456787,
0.06246910244226456,
-0.025417378172278404,
0.042557358741760254,
0.005040111020207405,
0.048327699303627014,
-0.052041664719581604,
0.07205542176961899,
-0.15317866206169128,
0.04429653659462929,
0.06145373359322548,
0.025780342519283295,
-0.007735091261565685,
0.0074270498007535934,
0.0409400649368763,
0.05626263469457626,
-0.004340629559010267,
0.04183301702141762,
-0.004338904283940792,
0.013491062447428703,
0.01256636157631874,
0.05396139994263649,
-0.019831445068120956,
-0.002288481220602989,
0.03254835307598114,
-0.009147332049906254,
0.029820650815963745,
0.01189787033945322,
-0.02527000568807125,
-0.019067347049713135,
0.04924687370657921,
-0.012182620353996754,
0.044721029698848724,
-0.014506002888083458,
-0.008886867202818394,
0.022352172061800957,
-0.05094894394278526,
-0.026425616815686226,
-0.04577186703681946,
0.029338734224438667,
-0.014703079126775265,
-0.04136968404054642,
-0.033935125917196274,
-0.009878803975880146,
-0.032911673188209534,
0.4183911085128784,
-0.00873122550547123,
0.04569987580180168,
-0.08518782258033752,
0.029421914368867874,
-0.0029683334287256002,
-0.06204571574926376,
0.008127622306346893,
-0.06721231341362,
-0.01987207867205143,
0.01827775500714779,
0.04784073680639267,
-0.013051214627921581,
0.09059566259384155,
-0.06616479903459549,
-0.00811977032572031,
0.024333812296390533,
0.056140851229429245,
0.004898525774478912,
0.0014731991104781628,
0.04870736598968506,
-0.0424010306596756,
-0.03398625925183296,
-0.022632667794823647,
-0.025761496275663376,
0.0029732235707342625,
0.0019308022456243634,
0.0003717740182764828,
0.08879158645868301,
0.04052275791764259,
-0.011080678552389145,
0.0540497750043869,
0.015677116811275482,
-0.057147420942783356,
0.00024589046370238066,
-0.022278089076280594,
-0.028929168358445168,
0.07792771607637405,
0.008667754009366035,
0.011957951821386814,
0.015304967761039734,
0.0041091954335570335,
-0.06034717336297035,
0.04087110981345177,
-0.11031052470207214,
-0.0905691459774971,
0.0789521262049675,
0.02000030130147934,
-0.014285384677350521,
-0.05068881809711456,
0.014669026248157024,
-0.05443132296204567,
-0.007134721614420414,
-0.0324019193649292,
0.05432773754000664,
-0.02774200588464737,
0.026083271950483322,
-0.037354182451963425,
0.004563556984066963,
-0.007784589193761349,
-0.07283314317464828,
-0.04901385307312012,
-0.04971599578857422,
-0.010985936038196087,
0.02386746183037758,
0.060580793768167496,
0.044956594705581665,
-0.04655962437391281,
-0.028848690912127495,
0.007097907830029726,
0.001573543413542211,
0.012172579765319824,
-0.004324452951550484,
-0.007469588425010443,
-0.04266350343823433,
0.0053268480114638805,
-0.025396469980478287,
0.045799244195222855,
-0.017518214881420135,
0.05614953115582466,
-0.025750083848834038,
0.04659029096364975,
0.00044312517275102437,
0.026823827996850014,
-0.030608272179961205,
-0.005211411509662867,
0.021710792556405067,
-0.06011580303311348,
-0.01834121160209179,
0.04605971276760101,
0.08636990934610367,
-0.033378105610609055,
-0.008721206337213516,
-0.09568633884191513,
-0.010507633909583092,
-0.014377826824784279,
-0.010934516787528992,
0.00039601020398549736,
0.028696119785308838,
-0.014760713092982769,
-0.001848147134296596,
0.061167407780885696,
0.031800221651792526,
0.017857082188129425,
0.0332469716668129,
-0.010236380621790886,
0.017001064494252205,
-0.049245040863752365,
-0.03661225736141205,
0.10801219940185547,
-0.022369809448719025,
0.018657078966498375,
-0.020884215831756592,
0.018521741032600403,
-0.0006533128907904029,
-0.059367988258600235,
-0.005601085256785154,
-0.03225286677479744,
0.02718101441860199,
0.0747794434428215,
-0.02034396305680275,
-0.013219851069152355,
0.022315260022878647,
0.028006065636873245,
-0.28630730509757996,
-0.012108054012060165,
0.035876549780368805,
-0.02401595562696457,
0.019286053255200386,
-0.034825894981622696,
0.08904082328081131,
-0.01350047904998064,
0.01470482163131237,
-0.061504583805799484,
0.06546951085329056,
0.028790360316634178,
-0.06121034547686577,
0.00981512013822794,
0.03687463700771332,
0.12907668948173523,
0.0472259446978569,
-0.05635799840092659,
0.02733432687819004,
0.00017797152395360172,
0.0002488421741873026,
-0.015140927396714687,
-0.0379733145236969,
0.011074625886976719,
0.0011158965062350035,
-0.034434664994478226,
0.10162676125764847,
-0.013346121646463871,
-0.0037023616023361683,
0.007298766169697046,
-0.014600450173020363,
0.0046765245497226715,
-0.024548621848225594,
-0.0765041708946228,
-0.009868023917078972,
-0.06162508577108383,
0.026117226108908653,
0.05045538768172264,
-0.022641729563474655,
-0.03556317463517189,
-0.055016033351421356,
0.0051689352840185165,
0.023700138553977013,
-0.02572646364569664,
-0.03980192914605141,
-0.05381802096962929,
0.0282400231808424,
0.07726767659187317,
-0.049137137830257416,
-0.039823535829782486,
0.010323328897356987,
0.011620777659118176,
0.022081926465034485,
-0.05690015107393265,
0.05834612622857094,
-0.047923412173986435,
-0.07234744727611542,
0.006589858792722225,
-0.06253905594348907,
0.016867943108081818,
0.028801333159208298,
0.04240833967924118,
0.023593954741954803,
-0.008568882942199707,
-0.029897207394242287,
0.0499260351061821,
0.07170640677213669,
0.00007019170880084857,
0.05492011085152626,
-0.029065843671560287,
0.012601379305124283,
0.12074211984872818,
-0.053591519594192505,
-0.02438216283917427,
0.056126613169908524,
0.005991119425743818,
-0.009572668932378292,
-0.004742078948765993,
-0.037058744579553604,
0.010663741268217564,
-0.0286101084202528,
-0.07159151881933212,
0.04228157922625542,
0.012812678702175617,
-0.05531740561127663,
0.011045413091778755,
0.034115757793188095,
-0.008791585452854633,
0.003986544907093048,
0.03116687200963497,
-0.009070913307368755,
0.009166504256427288,
-0.03388245776295662,
-0.014443185180425644,
0.032768283039331436,
0.009842143394052982,
-0.25484925508499146,
-0.04423125460743904,
0.048341281712055206,
-0.053995221853256226,
-0.02882038801908493,
0.03533771634101868,
0.009241464547812939,
-0.004380169790238142,
-0.09157875925302505,
-0.0000588249386055395,
-0.007987136952579021,
0.02202070876955986,
0.03753230348229408,
0.03251774609088898,
-0.07204514741897583,
0.014306840486824512,
0.021948374807834625,
-0.022876780480146408,
0.040568526834249496,
-0.013541712425649166,
0.006024830974638462,
0.0012330198660492897,
0.13298118114471436,
-0.004794911481440067,
-0.0415596142411232,
0.005063278134912252,
0.02181529439985752,
0.04712352529168129,
-0.03709317743778229,
-0.012981925159692764,
-0.020124176517128944,
-0.02519301325082779,
0.023900361731648445,
-0.037183236330747604,
-0.017122210934758186,
0.02278957888484001,
-0.016557365655899048,
-0.000768967205658555,
0.008287636563181877,
-0.036749932914972305,
-0.033243484795093536,
-0.019590342417359352,
-0.0029005538672208786,
-0.06809169799089432,
0.03745843097567558,
-0.0836825743317604,
-0.026174599304795265,
-0.0032099690288305283,
-0.010938853956758976,
0.009236697107553482,
-0.017259616404771805,
-0.02399819903075695,
0.02773866057395935,
0.08017458021640778,
-0.003209254937246442,
0.0270369965583086,
-0.03576348349452019,
-0.022315016016364098,
0.02059200592339039,
-0.018518611788749695,
-0.029496926814317703,
-0.006146179977804422,
-0.07945572584867477,
0.03370609134435654,
0.07310228049755096
] |
PMID:18640
|
Allergic granulomatosis and angiitis (Churg-Strauss syndrome). Report and analysis of 30 cases.
|
The clinical and morphologic findings of allergic granulomatosis and angiitis of Churg and Strauss in 21 men and 9 women were reviewed. The classic features are those of systemic vasculitis in a setting of bronchial asthma and eosinophilia. Pathologically there is necrotizing vasculitis of small arteries and veins with extravascular granulomas, and infiltration of vessels and perivascular tissues with eosinophilia. These features differentiate it from poly-arteritis nodosa. The lungs, peripheral nerves, and skin are most frequently involved. Renal failure was encountered in only one patient in this series. Shortness of the interval from onset of asthma to appearance of vasculitis is an unfavorable prognostic sign. Corticosteroids seem to influence long-term survival favorably.
|
Allergic granulomatosis and angiitis (Churg-Strauss syndrome). Report and analysis of 30 cases. The clinical and morphologic findings of allergic granulomatosis and angiitis of Churg and Strauss in 21 men and 9 women were reviewed. The classic features are those of systemic vasculitis in a setting of bronchial asthma and eosinophilia. Pathologically there is necrotizing vasculitis of small arteries and veins with extravascular granulomas, and infiltration of vessels and perivascular tissues with eosinophilia. These features differentiate it from poly-arteritis nodosa. The lungs, peripheral nerves, and skin are most frequently involved. Renal failure was encountered in only one patient in this series. Shortness of the interval from onset of asthma to appearance of vasculitis is an unfavorable prognostic sign. Corticosteroids seem to influence long-term survival favorably.
|
[
-0.01194375567138195,
0.0015199247281998396,
0.013296584598720074,
0.06601336598396301,
-0.00869496539235115,
-0.022509053349494934,
0.07650654762983322,
0.0479978546500206,
-0.012322134338319302,
-0.04762158542871475,
-0.057182151824235916,
-0.0068254354409873486,
0.020284561440348625,
-0.016093265265226364,
-0.017278026789426804,
0.01788409799337387,
0.01949065923690796,
0.03750063478946686,
-0.09543465077877045,
0.029543789103627205,
-0.07588575780391693,
0.0423111654818058,
0.02035781368613243,
-0.03197986260056496,
-0.005585414823144674,
0.0030778434593230486,
0.0641452819108963,
-0.009954883717000484,
0.015620561316609383,
-0.11838198453187943,
0.0023968988098204136,
-0.04977595806121826,
-0.03894143924117088,
0.026817679405212402,
-0.012435990385711193,
-0.03904765471816063,
-0.0925971046090126,
0.039596978574991226,
-0.03603145852684975,
0.024559414014220238,
0.03710190951824188,
-0.003773454809561372,
-0.005893828347325325,
0.0014121568528935313,
-0.025523370131850243,
-0.027423391118645668,
-0.05011623352766037,
0.04605971276760101,
0.06836874783039093,
0.04022762179374695,
0.019641656428575516,
-0.07474540174007416,
0.019647467881441116,
0.09965891391038895,
-0.00402428675442934,
0.013675738126039505,
-0.006006747018545866,
0.06343446671962738,
-0.0001704288151813671,
-0.027428103610873222,
0.02399345301091671,
-0.01721273362636566,
-0.09686832129955292,
0.07575450837612152,
0.031069105491042137,
-0.04288165271282196,
0.025973137468099594,
-0.014766654931008816,
-0.001991870580241084,
0.024849101901054382,
0.04181749373674393,
0.07764028757810593,
-0.039313722401857376,
0.038373637944459915,
-0.033705323934555054,
0.05307741463184357,
-0.06450039148330688,
-0.04354871064424515,
0.04989265650510788,
0.06513602286577225,
0.04297392815351486,
0.08260626345872879,
-0.011699759401381016,
-0.03293018415570259,
-0.019688371568918228,
-0.04612747207283974,
-0.006250911857932806,
-0.07765884697437286,
-0.01618639938533306,
0.02262570895254612,
0.009458224289119244,
-0.05046847090125084,
-0.023005079478025436,
0.03297179192304611,
-0.04193878546357155,
0.0314156673848629,
-0.07525469362735748,
0.007736130617558956,
-0.0388760082423687,
0.4052157998085022,
-0.009268173947930336,
-0.04970448091626167,
0.017779618501663208,
-0.03490594029426575,
0.004681323189288378,
0.017796888947486877,
-0.05738132819533348,
-0.06598956882953644,
-0.011545775458216667,
0.022427856922149658,
0.012439754791557789,
-0.07383972406387329,
0.036106862127780914,
-0.007688124198466539,
-0.01648878864943981,
-0.05222416669130325,
0.08136078715324402,
0.00783055555075407,
-0.06643877923488617,
0.03163952752947807,
0.04070541262626648,
-0.06183654069900513,
0.00581237580627203,
-0.010849141515791416,
0.05034241825342178,
0.021123260259628296,
0.05263368412852287,
0.0658854991197586,
0.042954351752996445,
-0.008239909075200558,
0.11416768282651901,
0.03306074067950249,
-0.02481974847614765,
-0.022266684100031853,
0.009439334273338318,
-0.02077905461192131,
0.0667351633310318,
0.08531425893306732,
0.003307580715045333,
0.07537701725959778,
-0.006444673519581556,
-0.09211059659719467,
-0.021697215735912323,
-0.06136897951364517,
-0.037183888256549835,
0.08828788250684738,
0.0026494532357901335,
-0.025691622868180275,
-0.049319714307785034,
0.049814920872449875,
0.0005892462213523686,
0.002050926676020026,
-0.028420569375157356,
0.05278219282627106,
-0.009984550066292286,
-0.02095562033355236,
-0.033274583518505096,
0.053506288677453995,
0.05203989893198013,
-0.007848299108445644,
-0.028142374008893967,
-0.038867510855197906,
0.0045065078884363174,
0.014364585280418396,
0.01947462186217308,
-0.036904044449329376,
-0.023310180753469467,
-0.016037581488490105,
0.029142562299966812,
0.0038669798523187637,
0.024197546765208244,
-0.004913670476526022,
0.03126952424645424,
-0.0359610877931118,
0.011447466909885406,
0.07224127650260925,
-0.006294040475040674,
-0.003572259098291397,
-0.022356605157256126,
-0.006123011931777,
-0.007473357953131199,
0.027781005948781967,
-0.00017736165318638086,
0.034282952547073364,
0.007423365954309702,
-0.0025757381226867437,
-0.03699576482176781,
-0.0297967791557312,
0.05791327357292175,
-0.02100016549229622,
-0.014747049659490585,
-0.033960554748773575,
-0.06595537811517715,
-0.018970925360918045,
0.011559204198420048,
-0.01588021032512188,
-0.02495156042277813,
-0.02902039885520935,
-0.0004378062440082431,
0.009292191825807095,
0.09074043482542038,
0.012226277962327003,
0.05390002578496933,
-0.0051828408613801,
0.013010663911700249,
0.0300512183457613,
-0.027933688834309578,
-0.01739722676575184,
0.00041518043144606054,
0.0010384138440713286,
-0.028715183958411217,
0.03413980454206467,
0.011795517057180405,
0.03302774950861931,
0.00816163420677185,
0.009347746148705482,
-0.022370189428329468,
-0.012297592125833035,
0.041268836706876755,
-0.01590009219944477,
-0.017859216779470444,
0.024421049281954765,
-0.04586879909038544,
-0.2758116126060486,
-0.05815844237804413,
-0.019091393798589706,
-0.048152774572372437,
-0.003256565425544977,
-0.0015384922735393047,
0.02919902466237545,
0.010515079833567142,
0.03011258877813816,
-0.008469253778457642,
0.004481102805584669,
0.08308989554643631,
-0.020720921456813812,
-0.044347502291202545,
0.033783771097660065,
0.09736984223127365,
0.05662000924348831,
-0.020768746733665466,
-0.01610024832189083,
0.048210516571998596,
-0.009532228112220764,
-0.0462539903819561,
-0.015266234055161476,
0.04650694876909256,
0.008266603574156761,
0.0006628509727306664,
0.1460922807455063,
-0.0034478683955967426,
-0.06431172043085098,
-0.07712820917367935,
0.04730908200144768,
0.041587766259908676,
-0.022227754816412926,
0.010596192441880703,
0.05750412866473198,
-0.02886076830327511,
-0.04823633283376694,
0.01877233013510704,
-0.04690068960189819,
0.020204072818160057,
-0.0704096108675003,
-0.05586839094758034,
-0.026407718658447266,
-0.0073400805704295635,
-0.038515906780958176,
0.014719621278345585,
0.024925516918301582,
0.047438010573387146,
-0.05136273056268692,
0.00314658903516829,
0.04626632481813431,
-0.027355598285794258,
0.031096715480089188,
0.01982254721224308,
0.010602572932839394,
-0.021926939487457275,
-0.013223816640675068,
0.02093341201543808,
-0.03838342800736427,
-0.03652746230363846,
-0.0034550302661955357,
-0.028836695477366447,
0.04450143128633499,
-0.025029797106981277,
-0.05033061280846596,
0.023950884118676186,
0.07407087087631226,
-0.0029637061525136232,
0.05985529348254204,
-0.02316906303167343,
-0.033917468041181564,
0.0990186259150505,
-0.03506981581449509,
-0.021697811782360077,
0.016022469848394394,
-0.006374559365212917,
0.0279412642121315,
0.0027145228814333677,
-0.017789620906114578,
0.054555878043174744,
0.05065068602561951,
-0.08672481030225754,
-0.0020713761914521456,
-0.001957533648237586,
-0.038377419114112854,
0.019544692710042,
-0.019495133310556412,
-0.0394212007522583,
-0.03720199316740036,
-0.016863303259015083,
-0.026744164526462555,
-0.03929433226585388,
-0.035734497010707855,
-0.01420819666236639,
-0.0041634440422058105,
0.05321146175265312,
-0.27411985397338867,
0.013210630044341087,
0.061326880007982254,
0.005147438030689955,
0.0012177105527371168,
-0.02323901280760765,
-0.047081105411052704,
-0.03368719667196274,
0.013819766230881214,
-0.007053352892398834,
0.10429798066616058,
-0.029262185096740723,
0.05683200806379318,
-0.007971439510583878,
-0.038898907601833344,
0.0364852100610733,
0.04677240177989006,
-0.014046070165932178,
-0.0013102778466418386,
-0.04619774967432022,
-0.01501391176134348,
-0.06126674264669418,
0.13842304050922394,
0.04321333020925522,
-0.009662876836955547,
0.011404960416257381,
0.01905996724963188,
0.06199457496404648,
-0.09359665215015411,
0.042118139564991,
0.024246620014309883,
-0.0667867586016655,
0.06513983011245728,
0.05652144178748131,
0.013937306590378284,
0.006356311962008476,
-0.04328123480081558,
0.06746646016836166,
0.0012906695483252406,
0.033319611102342606,
-0.0029375047888606787,
-0.03392036259174347,
-0.043345268815755844,
0.0033527554478496313,
-0.008877364918589592,
-0.03455450013279915,
-0.040619704872369766,
0.01796122081577778,
0.03470207750797272,
-0.008055537939071655,
-0.05483393371105194,
-0.07893266528844833,
0.0478198304772377,
0.08359834551811218,
0.07140381634235382,
-0.023821374401450157,
0.007321374956518412,
-0.08767785876989365,
-0.0014901835238561034,
-0.029524559155106544,
0.038181230425834656,
0.03060346096754074,
-0.0995316356420517,
0.041173383593559265,
0.0602559857070446
] |
PMID:18647
|
Interrelationship between the dietary regulation of fatty acid synthesis and the fatty acyl-CoA desaturases.
|
In this paper we present further evidence for the close control of fatty acid synthetase and stearoyl-CoA desaturase. Furthermore, we have established that whereas dietary palmitic acid may influence the activity of this desaturase but not of fatty acid synthetase, dietary linoleic acid appears to control both these enzymes. Finally, we have studied the influence of dietary fat and carbohydrate on the activities of the delta6 and delta5 desaturases. The former is only slightly affected by these dietary components. The delta5 desaturase activity is stimulated as the dietary fat content rises but is unaffected by dietary carbohydrate. The control of these enzymes is therefore independent of the control of fatty acid synthetase and stearoyl-CoA desaturase. From the data presented, the magnitude of the controlling effect of polyunsaturated fatty acids on fatty acid synthetase and stearoyl-CoA desaturase activity is determined and its relevance to lipogenesis in man based on daily intake of carbohydrate and linoleic acid is discussed.
|
Interrelationship between the dietary regulation of fatty acid synthesis and the fatty acyl-CoA desaturases. In this paper we present further evidence for the close control of fatty acid synthetase and stearoyl-CoA desaturase. Furthermore, we have established that whereas dietary palmitic acid may influence the activity of this desaturase but not of fatty acid synthetase, dietary linoleic acid appears to control both these enzymes. Finally, we have studied the influence of dietary fat and carbohydrate on the activities of the delta6 and delta5 desaturases. The former is only slightly affected by these dietary components. The delta5 desaturase activity is stimulated as the dietary fat content rises but is unaffected by dietary carbohydrate. The control of these enzymes is therefore independent of the control of fatty acid synthetase and stearoyl-CoA desaturase. From the data presented, the magnitude of the controlling effect of polyunsaturated fatty acids on fatty acid synthetase and stearoyl-CoA desaturase activity is determined and its relevance to lipogenesis in man based on daily intake of carbohydrate and linoleic acid is discussed.
|
[
0.007458659820258617,
0.0037760110571980476,
-0.035204533487558365,
0.03194207698106766,
-0.014071322977542877,
-0.011047834530472755,
0.013364842161536217,
0.04230058193206787,
-0.04701250419020653,
-0.04910491406917572,
0.054208818823099136,
-0.10116615891456604,
0.010288409888744354,
-0.014538980089128017,
-0.016941118985414505,
-0.09420870989561081,
0.018079077824950218,
0.0006967118242755532,
-0.04672635719180107,
-0.012247906997799873,
0.055368054658174515,
0.008671633899211884,
0.05047241225838661,
-0.04674316942691803,
0.00498458044603467,
-0.009621329605579376,
0.04761042073369026,
-0.007034077774733305,
-0.0663255974650383,
-0.1894114762544632,
0.011051156558096409,
0.03551965206861496,
0.05644321069121361,
-0.08104198426008224,
-0.04788828268647194,
-0.039268139749765396,
-0.04502323269844055,
-0.02713952772319317,
-0.027598069980740547,
0.013763759285211563,
0.02200871892273426,
0.015177924185991287,
0.06463080644607544,
-0.013721509836614132,
0.012428988702595234,
0.0012624909868463874,
-0.03221210837364197,
0.03046763315796852,
0.04384562000632286,
0.0012658188352361321,
-0.005895834416151047,
0.04753576219081879,
0.03285687416791916,
0.002977122785523534,
0.05338757857680321,
0.028055990114808083,
-0.009847164154052734,
0.045307282358407974,
0.0006333001074381173,
0.016557468101382256,
-0.02276788279414177,
0.02791135199368,
-0.16571886837482452,
0.07443629205226898,
0.02907310612499714,
0.02282797545194626,
-0.016121549531817436,
-0.012460986152291298,
0.03081914409995079,
0.03614521399140358,
-0.10677047073841095,
0.05130051076412201,
-0.04236778989434242,
-0.0135935815051198,
0.08435922861099243,
-0.01601197011768818,
0.009775111451745033,
0.009852340444922447,
-0.04736467823386192,
-0.001355874934233725,
0.009926163591444492,
-0.01718217320740223,
0.03935011848807335,
0.006858064793050289,
-0.0625837966799736,
-0.04115954414010048,
0.019566521048545837,
-0.019929837435483932,
-0.016349149867892265,
0.025291157886385918,
-0.006308065727353096,
-0.05325162783265114,
0.04353991895914078,
-0.0033514483366161585,
-0.05807354301214218,
0.05314964801073074,
0.0394136980175972,
-0.04520310088992119,
-0.023286571726202965,
0.3988184630870819,
-0.02970324642956257,
0.0028681091498583555,
-0.047146402299404144,
0.004793735686689615,
-0.03559868037700653,
-0.08215872943401337,
-0.05014268308877945,
0.03955550119280815,
0.01582416705787182,
-0.014688310213387012,
0.052088167518377304,
0.028429467231035233,
0.017814895138144493,
-0.036333922296762466,
-0.030434027314186096,
-0.006762027274817228,
0.07044199109077454,
0.0028354323003441095,
-0.00789682473987341,
0.05501876026391983,
-0.008315191604197025,
0.031778253614902496,
0.01979474537074566,
0.014854196459054947,
0.023035133257508278,
-0.005254780407994986,
-0.011566766537725925,
0.10731024295091629,
0.02607683278620243,
-0.008145330473780632,
0.045983102172613144,
0.022196101024746895,
-0.0007957910420373082,
-0.010135975666344166,
-0.015119124203920364,
0.0039058076217770576,
0.09012486040592194,
-0.0178334042429924,
0.0052350182086229324,
0.030888479202985764,
-0.004099130630493164,
-0.012028582394123077,
0.008288253098726273,
-0.08313675969839096,
0.00932497251778841,
0.10472530871629715,
0.05003170669078827,
0.024144815281033516,
-0.01499459519982338,
0.02490476332604885,
0.02559601701796055,
-0.011212549172341824,
-0.009001269936561584,
0.03477037698030472,
0.00812136847525835,
0.0031984997913241386,
0.013757599517703056,
-0.019028987735509872,
0.03300846740603447,
-0.06433773785829544,
-0.05311666429042816,
-0.06892555952072144,
-0.021730035543441772,
0.0317864827811718,
0.047446321696043015,
-0.021915417164564133,
-0.06943175196647644,
0.013472260907292366,
-0.013249045237898827,
-0.039200663566589355,
0.0614023394882679,
-0.0432417206466198,
-0.029444824904203415,
-0.013421770185232162,
0.042851630598306656,
0.05240630730986595,
0.0047903889790177345,
0.019423948600888252,
-0.03791046515107155,
0.034232690930366516,
0.0939488634467125,
-0.051855169236660004,
-0.04089087247848511,
0.0255771242082119,
-0.033787500113248825,
-0.0581219419836998,
-0.08298049867153168,
-0.0012440825812518597,
0.0076376269571483135,
0.01603884808719158,
-0.048480257391929626,
-0.018076209351420403,
-0.07768181711435318,
0.012158659286797047,
-0.056359026581048965,
-0.09094325453042984,
-0.03216469660401344,
0.04032280296087265,
-0.016447462141513824,
-0.019578855484724045,
0.07059525698423386,
0.006687375716865063,
0.030166083946824074,
0.013992770574986935,
0.042103976011276245,
-0.027855675667524338,
-0.03810320422053337,
-0.03497214987874031,
0.1037156879901886,
0.04722283035516739,
-0.027398739010095596,
0.03126312419772148,
-0.02185520902276039,
0.03897922486066818,
-0.053704697638750076,
0.06338030844926834,
0.04576362669467926,
-0.010283241979777813,
0.05629409849643707,
0.01609906554222107,
0.062390051782131195,
0.014748877845704556,
0.020413976162672043,
-0.2995185852050781,
-0.05736029893159866,
0.00060919119277969,
0.02058533765375614,
0.009529988281428814,
0.011588744819164276,
0.017760470509529114,
-0.017833426594734192,
0.004286377690732479,
-0.027341041713953018,
-0.01987536810338497,
0.06620744615793228,
-0.027448104694485664,
0.03686411678791046,
-0.014111313037574291,
0.0006613809964619577,
0.013977915979921818,
-0.043398305773735046,
0.0029310989193618298,
0.030610913410782814,
-0.00025950741837732494,
-0.05410940945148468,
0.02162626013159752,
-0.015986235812306404,
0.09489909559488297,
-0.01783382147550583,
0.1087542474269867,
0.028655363246798515,
0.025812802836298943,
0.0005878496449440718,
0.04389095678925514,
0.09293944388628006,
-0.04365940019488335,
-0.10254450142383575,
-0.03124776855111122,
-0.01592404581606388,
-0.034210510551929474,
-0.06461174041032791,
0.03125602751970291,
-0.019905973225831985,
-0.00838190596550703,
0.0032444677781313658,
-0.03669609874486923,
-0.013815052807331085,
-0.07627474516630173,
-0.05213705077767372,
0.0016151095042005181,
0.0011464589042589068,
0.01595328375697136,
-0.022655481472611427,
0.03015240468084812,
0.053424473851919174,
0.014308375306427479,
0.0031492719426751137,
-0.06061186641454697,
-0.006586503237485886,
-0.020229583606123924,
-0.009864440187811852,
-0.05633259937167168,
-0.019777478650212288,
-0.06311246752738953,
0.008676225319504738,
0.00589894549921155,
0.02119472064077854,
-0.018446341156959534,
0.0368640273809433,
-0.0060095032677054405,
-0.020554332062602043,
0.007269416935741901,
0.044100500643253326,
0.01823526620864868,
0.044518448412418365,
-0.09062061458826065,
-0.0341985858976841,
0.03485322371125221,
0.03998807445168495,
-0.03438689187169075,
-0.03794180229306221,
-0.097316674888134,
-0.027115607634186745,
0.030189724639058113,
-0.03162331134080887,
0.07796164602041245,
-0.020166050642728806,
0.026321321725845337,
-0.009199255146086216,
0.04612436518073082,
-0.06430798023939133,
0.023131113499403,
0.024127444252371788,
0.031201019883155823,
-0.01772225648164749,
-0.03465690463781357,
-0.08492013067007065,
0.015024912543594837,
0.04695175588130951,
-0.2527224123477936,
0.07050622999668121,
0.01478432398289442,
-0.035760797560214996,
-0.0122207747772336,
0.006230929400771856,
0.03596757352352142,
0.03922819346189499,
-0.005958093795925379,
0.020338397473096848,
-0.02411867119371891,
-0.024344991892576218,
0.03669063001871109,
0.04532783478498459,
0.014360381290316582,
0.01938270591199398,
0.039160117506980896,
0.01788174919784069,
0.048890601843595505,
-0.04010742902755737,
-0.03538360074162483,
0.0022304437588900328,
0.1190185397863388,
0.02433607541024685,
-0.015400172211229801,
0.06799111515283585,
0.016021274030208588,
0.04049075394868851,
-0.0522279217839241,
0.048462387174367905,
-0.025000648573040962,
0.026062535122036934,
0.05481846630573273,
-0.011048497632145882,
0.007502819411456585,
0.08799660950899124,
-0.030680757015943527,
-0.026891350746154785,
-0.010121569968760014,
0.018981151282787323,
0.050873763859272,
-0.01938822492957115,
-0.023598510771989822,
-0.012450463138520718,
0.026770487427711487,
-0.040654256939888,
-0.006565912626683712,
-0.021942900493741035,
-0.04665190353989601,
-0.011900688521564007,
0.01887616701424122,
-0.062149763107299805,
0.014376924373209476,
0.036335960030555725,
0.016916794702410698,
-0.008385908789932728,
-0.041574038565158844,
0.011876211501657963,
-0.01566185988485813,
-0.004652345087379217,
0.043271344155073166,
0.019359957426786423,
-0.04637911543250084,
0.10670580714941025,
0.07869609445333481
] |
PMID:18648
|
Studies on the inhibition of the desaturases by cyclopropenoid fatty acids.
|
Unwashed rat liver microsomes were used to study the inhibition of the delta6 and delta9 desaturases by cyclopropenoid fatty acids with the ring structure about the 9,10 or 6,7 carbon atoms. The 9,10 cyclopropenoid acid (sterculic acid) is shown to be an effective inhibitor of only delta9 desaturase and then only in the presence of MgCl2 and coenzyme A (presumably due to the formation of sterculoyl-CoA). Two 6,7 cyclopropenoid acids of different chain lengths showed no marked inhibition of either the delta6 or delta9 desaturase. By the use of [3H]-sterculic acid, it has been shown that under conditions of high inhibition of the delta9 desaturase the inhibitor is not covalently attached to the enzyme at any point. This disproves older ideas on the mechanism of inhibition that assumed reaction between the cyclopropenoid ring and sulphydryl groups on the enzymes.
|
Studies on the inhibition of the desaturases by cyclopropenoid fatty acids. Unwashed rat liver microsomes were used to study the inhibition of the delta6 and delta9 desaturases by cyclopropenoid fatty acids with the ring structure about the 9,10 or 6,7 carbon atoms. The 9,10 cyclopropenoid acid (sterculic acid) is shown to be an effective inhibitor of only delta9 desaturase and then only in the presence of MgCl2 and coenzyme A (presumably due to the formation of sterculoyl-CoA). Two 6,7 cyclopropenoid acids of different chain lengths showed no marked inhibition of either the delta6 or delta9 desaturase. By the use of [3H]-sterculic acid, it has been shown that under conditions of high inhibition of the delta9 desaturase the inhibitor is not covalently attached to the enzyme at any point. This disproves older ideas on the mechanism of inhibition that assumed reaction between the cyclopropenoid ring and sulphydryl groups on the enzymes.
|
[
-0.05118602141737938,
-0.03012288548052311,
-0.04739990830421448,
0.04460294544696808,
-0.02803185023367405,
-0.011866547167301178,
0.022588860243558884,
0.04658089205622673,
-0.04056543856859207,
-0.03675289824604988,
0.07530470192432404,
-0.043041326105594635,
0.0022468629758805037,
0.004120689816772938,
-0.02926757000386715,
-0.05857988819479942,
0.01923440396785736,
0.018486984074115753,
-0.043535634875297546,
-0.0037532153073698282,
-0.011100320145487785,
0.0054649049416184425,
0.019729405641555786,
-0.004763266071677208,
0.03433896601200104,
-0.004611663520336151,
0.0026413248851895332,
-0.0037890144158154726,
-0.08477757126092911,
-0.1921463906764984,
-0.015042221173644066,
-0.011119779199361801,
0.04057007282972336,
-0.06047428399324417,
-0.013916141353547573,
-0.00956916343420744,
0.0029344437643885612,
0.02289479970932007,
-0.025736432522535324,
0.026238320395350456,
0.0527619794011116,
0.022183241322636604,
0.020435214042663574,
-0.004286613315343857,
0.012884163297712803,
-0.003578240517526865,
-0.01728557050228119,
0.006588129326701164,
0.08939787745475769,
-0.03137841448187828,
0.008320203050971031,
0.007908763363957405,
0.01576157659292221,
0.022966044023633003,
0.00810698326677084,
0.01851688325405121,
-0.0005048168241046369,
0.032302532345056534,
0.007879278622567654,
0.0061792670749127865,
0.014934285543859005,
0.001816762494854629,
-0.14135858416557312,
0.045191440731287,
0.023095840588212013,
0.035343557596206665,
0.0017766031669452786,
-0.035393137484788895,
0.007937762886285782,
0.06285148113965988,
-0.09822230786085129,
-0.0045369877479970455,
0.006675605662167072,
-0.0006135932053439319,
0.053150128573179245,
-0.009046596474945545,
-0.012281740084290504,
-0.04980919510126114,
-0.0022988845594227314,
-0.04378123581409454,
0.019095364958047867,
-0.025073228403925896,
0.07171423733234406,
-0.007984892465174198,
0.0025541516952216625,
0.0138173159211874,
0.017168821766972542,
0.004639012273401022,
0.00919754896312952,
-0.011427036486566067,
0.035885103046894073,
-0.03707011044025421,
0.019503379240632057,
-0.04423970729112625,
-0.08657771348953247,
0.024735277518630028,
0.03827638179063797,
-0.03373362869024277,
-0.009838028810918331,
0.3543635904788971,
-0.0467040091753006,
0.021028343588113785,
-0.07491955161094666,
-0.02915903739631176,
0.008749956265091896,
-0.04142756387591362,
-0.017418164759874344,
-0.01714548096060753,
-0.0038089833687990904,
-0.019133182242512703,
0.052587058395147324,
0.0634041354060173,
0.0012808340834453702,
-0.06161809712648392,
-0.018277332186698914,
-0.005190844181925058,
0.0621371828019619,
-0.024692127481102943,
-0.04542781412601471,
0.015201158821582794,
0.009410412050783634,
0.025106532499194145,
0.008549082092940807,
0.009771699085831642,
0.04593447223305702,
-0.01615266688168049,
-0.022171178832650185,
0.07751363515853882,
0.09766971319913864,
0.02577388659119606,
0.041602734476327896,
-0.026204325258731842,
0.07567755877971649,
-0.016288094222545624,
-0.03654899075627327,
0.02192741446197033,
0.04941530153155327,
-0.023239614441990852,
-0.01918359473347664,
-0.013001235201954842,
-0.061871908605098724,
-0.02557196281850338,
0.041621752083301544,
-0.03841622918844223,
0.033373065292835236,
0.11800901591777802,
0.048722460865974426,
0.03124289959669113,
-0.046207789331674576,
0.06419558078050613,
0.02794009819626808,
-0.05165928602218628,
-0.04125399515032768,
0.03189094364643097,
-0.005752505734562874,
0.05332145094871521,
-0.045145824551582336,
-0.0026997169479727745,
-0.028910819441080093,
-0.009622844867408276,
-0.02341211773455143,
-0.035164859145879745,
-0.017167354002594948,
0.0940476506948471,
0.018209442496299744,
-0.02389352396130562,
-0.0500207245349884,
0.0011093550128862262,
0.0015460733557119966,
-0.033743973821401596,
0.023939363658428192,
-0.0117960125207901,
-0.013053548522293568,
-0.03533652052283287,
-0.011581170372664928,
0.03777144104242325,
0.030664561316370964,
-0.01230943575501442,
0.0152968131005764,
0.017769528552889824,
0.043385449796915054,
-0.03752287104725838,
-0.05744471773505211,
0.02131800539791584,
-0.02256767265498638,
-0.07015137374401093,
-0.0889737457036972,
-0.029021847993135452,
0.04040702432394028,
0.014532563276588917,
-0.07159090787172318,
-0.0017178121488541365,
-0.08480378985404968,
0.052242666482925415,
-0.09094996750354767,
-0.0995187982916832,
-0.06268487870693207,
0.044955264776945114,
0.0006581639172509313,
0.026320628821849823,
0.12117969989776611,
-0.0017096948577091098,
0.013530442491173744,
0.06560700386762619,
0.01709732785820961,
0.01676543615758419,
-0.03289404883980751,
-0.013255953788757324,
0.028592322021722794,
-0.0001628871395951137,
-0.030009986832737923,
0.026152674108743668,
-0.027982361614704132,
0.0464852973818779,
-0.025576099753379822,
0.06031308323144913,
0.019231146201491356,
0.03405173495411873,
0.027878908440470695,
0.015425042249262333,
0.05628380924463272,
-0.03500068187713623,
0.01718098670244217,
-0.3035939037799835,
-0.0563860684633255,
-0.01303213369101286,
-0.0008108001784421504,
0.05373982712626457,
-0.01862415112555027,
0.03034139797091484,
-0.0042991843074560165,
-0.05374141037464142,
-0.031089093536138535,
0.03081841953098774,
0.06628856062889099,
-0.05652231723070145,
-0.03193085640668869,
-0.05814637243747711,
-0.006289634853601456,
0.002170207677409053,
-0.04899747297167778,
0.051118139177560806,
0.020491503179073334,
0.0034642175305634737,
-0.05705887824296951,
-0.00530701270326972,
0.029077067971229553,
0.07921500504016876,
-0.002920877654105425,
0.13136297464370728,
0.03434523567557335,
0.030198341235518456,
0.0431542731821537,
0.03982670605182648,
0.09280344098806381,
-0.05682717636227608,
-0.05580392852425575,
-0.009678833186626434,
-0.037836022675037384,
-0.008127686567604542,
-0.08862587064504623,
0.06911469995975494,
-0.04275389015674591,
0.003746911883354187,
0.03841564804315567,
-0.030978580936789513,
-0.03144159913063049,
-0.06821074336767197,
-0.034351859241724014,
-0.0004293512902222574,
0.028949297964572906,
0.016515444964170456,
-0.025714917108416557,
0.004077838733792305,
0.08699266612529755,
-0.007631915621459484,
0.022210773080587387,
0.03778159245848656,
0.025594765320420265,
-0.024134309962391853,
0.008721880614757538,
-0.052410997450351715,
-0.005872745532542467,
-0.04497059807181358,
0.024839259684085846,
0.0015098936855793,
0.022297261282801628,
-0.026454105973243713,
-0.0010944668902084231,
-0.010500447824597359,
-0.03763459995388985,
0.087977834045887,
0.03238268569111824,
0.016009150072932243,
0.06679144501686096,
-0.0626840591430664,
-0.035254668444395065,
0.07394301891326904,
0.013575558550655842,
-0.010930330492556095,
-0.05381172150373459,
-0.07712012529373169,
0.022519731894135475,
-0.0210813470184803,
-0.07332172244787216,
0.029424874112010002,
-0.05834759771823883,
-0.008476341143250465,
0.009888658300042152,
0.055958688259124756,
-0.065493643283844,
0.04664555937051773,
0.030587241053581238,
0.07297738641500473,
0.004841936286538839,
-0.012099800631403923,
-0.011625527404248714,
0.062017641961574554,
-0.0026197663974016905,
-0.27280837297439575,
0.036001723259687424,
0.03522392734885216,
0.00817585177719593,
-0.03933894634246826,
0.060376137495040894,
0.05722396448254585,
-0.0018271978478878736,
0.008862930350005627,
0.019218629226088524,
0.00013739318819716573,
0.03953247144818306,
0.021189860999584198,
0.04329323023557663,
-0.0050692870281636715,
-0.029406100511550903,
0.05325615033507347,
0.02598552033305168,
0.027981247752904892,
-0.0037895613349974155,
-0.02655167318880558,
-0.04452164098620415,
0.1285727620124817,
0.03588106855750084,
-0.0009801938431337476,
0.074128158390522,
-0.018738072365522385,
0.0201420858502388,
-0.07746638357639313,
0.008526974357664585,
-0.06125754117965698,
0.00038071366725489497,
0.042773280292749405,
-0.08534940332174301,
-0.0524161197245121,
0.06072640046477318,
0.02519427239894867,
-0.017811980098485947,
0.014438641257584095,
0.0005103261210024357,
0.07397995889186859,
-0.0010348627110943198,
-0.04444429650902748,
-0.021775031462311745,
0.059589553624391556,
-0.07374449819326401,
-0.04879796877503395,
0.03711453825235367,
-0.006305835675448179,
0.011612917296588421,
0.03646256774663925,
-0.018813863396644592,
0.01031218096613884,
-0.03128622844815254,
-0.008325591683387756,
0.011452443897724152,
0.0019379662116989493,
-0.0032511285971850157,
-0.00048603571485728025,
-0.04679979383945465,
-0.02155786193907261,
-0.009785725735127926,
-0.02552364394068718,
0.11225854605436325,
0.09618815779685974
] |
PMID:18651
|
Penicillin combinations against multi-resistant urinary pathogens as an alternative to gentamycin treatment.
|
A total of 42 multi-resistant urinary pathogens, sensitive to gentamycin only, were investigated with regard to the effects of dicloxacillin and ampicillin or cloxacillin/flucloxacillin and amoxycillin in combination. A synergistic antibiotic effect with resulting low MIC levels was demonstrated for 81% of the strains. It was also found that a further increase in sensitivity might be achieved by adjustment of the pH level.
|
Penicillin combinations against multi-resistant urinary pathogens as an alternative to gentamycin treatment. A total of 42 multi-resistant urinary pathogens, sensitive to gentamycin only, were investigated with regard to the effects of dicloxacillin and ampicillin or cloxacillin/flucloxacillin and amoxycillin in combination. A synergistic antibiotic effect with resulting low MIC levels was demonstrated for 81% of the strains. It was also found that a further increase in sensitivity might be achieved by adjustment of the pH level.
|
[
-0.027103878557682037,
-0.033572711050510406,
-0.004450798965990543,
0.011704010888934135,
0.057120431214571,
-0.0052372729405760765,
-0.013997462578117847,
0.07338949292898178,
-0.0323919877409935,
-0.05701034143567085,
-0.003575454233214259,
-0.020516248419880867,
0.08490931987762451,
0.028825413435697556,
-0.007200673222541809,
-0.039268314838409424,
-0.027408508583903313,
-0.037717320024967194,
-0.017487216740846634,
-0.0006254580803215504,
-0.011583808809518814,
0.03163861110806465,
0.01656305603682995,
-0.030645662918686867,
0.020109841600060463,
-0.0338435098528862,
0.05052618682384491,
-0.01913423463702202,
-0.0498090535402298,
-0.2140098512172699,
0.027757758274674416,
0.04053278639912605,
-0.04972795397043228,
-0.03211737051606178,
0.004105060826987028,
0.015085885301232338,
-0.01353264320641756,
-0.0245115514844656,
-0.022530077025294304,
0.028346795588731766,
0.025118937715888023,
0.06252370029687881,
0.045481231063604355,
0.032696012407541275,
-0.017255162820219994,
-0.04189270734786987,
-0.07160298526287079,
0.04778224974870682,
0.16238921880722046,
0.021282846108078957,
0.0033417055383324623,
-0.06151136755943298,
0.023194188252091408,
0.07982908934354782,
0.012067975476384163,
-0.034252941608428955,
-0.025656849145889282,
-0.001508271787315607,
-0.041910380125045776,
-0.00473217386752367,
0.03355156630277634,
0.059320807456970215,
-0.12817661464214325,
0.06038042902946472,
-0.006581353489309549,
-0.019119014963507652,
-0.02428385056555271,
-0.020373187959194183,
0.07468056678771973,
0.0494084432721138,
-0.08196544647216797,
0.01649290695786476,
-0.04683297500014305,
0.03455246239900589,
0.018397584557533264,
0.0208531953394413,
-0.022113356739282608,
-0.04588797688484192,
0.013291437178850174,
0.013329719193279743,
-0.03664075955748558,
-0.0019381714519113302,
0.0007110098376870155,
-0.03789747506380081,
-0.010747473686933517,
-0.020891377702355385,
0.019926555454730988,
-0.061858806759119034,
0.009375548921525478,
0.014664278365671635,
0.0025070453993976116,
0.03356791287660599,
0.017448054626584053,
-0.06281812489032745,
-0.014713875018060207,
0.014151281677186489,
0.03738582879304886,
-0.0265604630112648,
-0.005751652643084526,
0.3926697075366974,
-0.05743049457669258,
-0.07006727904081345,
-0.019631579518318176,
-0.02711874060332775,
0.03782716393470764,
-0.019994588568806648,
0.05880636349320412,
-0.04523523524403572,
0.023648008704185486,
0.028207147493958473,
0.04328042268753052,
-0.033622901886701584,
0.011658578179776669,
-0.008560781367123127,
-0.03685065731406212,
0.02287975884974003,
0.0443190261721611,
-0.007574602495878935,
-0.004832426551729441,
-0.021107640117406845,
0.0050411163829267025,
-0.0989803895354271,
0.025147628039121628,
-0.0009718770743347704,
0.049749210476875305,
-0.023210538551211357,
0.0014803213998675346,
0.09049825370311737,
0.0006391284405253828,
-0.026994166895747185,
-0.01808106154203415,
0.016057061031460762,
-0.00806839857250452,
-0.04063499718904495,
0.011485118418931961,
0.025253986939787865,
-0.013276472687721252,
-0.057116780430078506,
0.04566796123981476,
0.03685590624809265,
-0.05510781332850456,
-0.09496411681175232,
-0.019852112978696823,
-0.08563277870416641,
-0.016840843483805656,
0.08835112303495407,
-0.03687993064522743,
0.01982233300805092,
-0.02232634648680687,
-0.035223398357629776,
-0.03279397636651993,
0.03816498443484306,
-0.02793619967997074,
-0.00696426210924983,
0.0050921509973704815,
0.04286991059780121,
0.046960245817899704,
-0.03926466405391693,
0.05051463469862938,
0.026042679324746132,
-0.05107207968831062,
-0.018451664596796036,
-0.02765163592994213,
0.11476306617259979,
-0.03269915282726288,
-0.046117525547742844,
-0.009167073294520378,
-0.021142691373825073,
-0.016959965229034424,
0.004091683775186539,
-0.013918695971369743,
-0.010639900341629982,
0.043219149112701416,
-0.06997673958539963,
-0.08298584073781967,
-0.014660011976957321,
-0.033450838178396225,
-0.04958115145564079,
0.013726508244872093,
0.03751424700021744,
-0.0019499505870044231,
0.08697880059480667,
-0.0187766645103693,
0.07055731117725372,
0.0300596971064806,
0.0020494370255619287,
-0.04244208708405495,
0.03831136226654053,
-0.00026590368361212313,
0.026397475972771645,
-0.016398761421442032,
0.023346293717622757,
-0.05182787775993347,
0.04535669833421707,
-0.001970707904547453,
0.015219924040138721,
-0.07927282899618149,
0.02503294311463833,
0.058347996324300766,
0.016576599329710007,
-0.0003155641898047179,
0.017169378697872162,
0.048607274889945984,
-0.003777338657528162,
0.020423490554094315,
0.00463377358391881,
0.02563788741827011,
-0.048393528908491135,
0.05449990928173065,
0.003492412157356739,
-0.02969961054623127,
0.027108553797006607,
0.04246871545910835,
0.015515489503741264,
-0.06514285504817963,
0.03857390210032463,
-0.02029050700366497,
0.024336807429790497,
0.018385879695415497,
0.010459739714860916,
0.03940785676240921,
-0.012621776200830936,
-0.0398811474442482,
-0.29855024814605713,
0.04783787205815315,
0.004457660485059023,
0.01990489661693573,
0.08260533958673477,
-0.08886805176734924,
-0.03247905522584915,
-0.036134056746959686,
-0.09125525504350662,
0.017790429294109344,
0.03764430060982704,
0.0285840705037117,
-0.014836124144494534,
0.015781715512275696,
-0.01732238195836544,
0.004716604482382536,
-0.03199081867933273,
-0.01532771810889244,
0.018184145912528038,
0.001987743889912963,
0.042375870048999786,
-0.016897520050406456,
0.045452531427145004,
0.014501012861728668,
0.06431987881660461,
0.03034135140478611,
0.07293318957090378,
-0.0003879100549966097,
0.001017990754917264,
0.021799301728606224,
-0.012940458953380585,
0.025519724935293198,
0.006663540843874216,
-0.09318798780441284,
0.028233129531145096,
0.06122231110930443,
0.02271886356174946,
-0.03272087499499321,
0.017244461923837662,
-0.04440515860915184,
-0.04487001895904541,
0.07747793942689896,
-0.03230072930455208,
-0.05354638025164604,
-0.03972591832280159,
-0.01020911242812872,
-0.029523110017180443,
0.06480936706066132,
-0.0131679717451334,
0.027398187667131424,
-0.052574947476387024,
0.026697983965277672,
0.04665139317512512,
0.008180255070328712,
0.0032789360266178846,
0.022310618311166763,
-0.02999737299978733,
-0.004996513016521931,
-0.06733082979917526,
-0.047641415148973465,
-0.018140412867069244,
-0.01775921881198883,
0.07261603325605392,
0.02049856260418892,
-0.021373175084590912,
-0.052972014993429184,
-0.0025074370205402374,
-0.018792644143104553,
0.04458136484026909,
0.02854357659816742,
0.06392116844654083,
0.13765430450439453,
-0.00752985430881381,
0.03092995285987854,
0.010931558907032013,
0.05370337888598442,
-0.004083607345819473,
-0.04556141048669815,
-0.08770401775836945,
-0.004695601295679808,
-0.03913261741399765,
-0.027930229902267456,
-0.005244551692157984,
-0.037165600806474686,
-0.01795395463705063,
-0.01904289796948433,
-0.007723705843091011,
0.04994561895728111,
0.07489282637834549,
0.006241944618523121,
-0.010336903855204582,
-0.05154421180486679,
0.08740264177322388,
0.005673271603882313,
-0.00884216371923685,
0.011706473305821419,
-0.2487577646970749,
0.02711131051182747,
0.032722726464271545,
0.03716211020946503,
0.01268896646797657,
0.07857180386781693,
0.0006818013498559594,
-0.08112166076898575,
-0.03211203217506409,
-0.0007150552701205015,
0.07700411230325699,
0.015600480139255524,
0.027551788836717606,
-0.0017093047499656677,
0.0368243008852005,
0.012872248888015747,
0.08417120575904846,
-0.0033625541254878044,
0.0021875854581594467,
0.020799897611141205,
0.04068547114729881,
0.01643412373960018,
0.13205170631408691,
-0.0260453000664711,
-0.015294425189495087,
0.040256183594465256,
0.009278169833123684,
0.011387667618691921,
-0.05123871937394142,
0.00954693928360939,
-0.017167629674077034,
0.05553746968507767,
0.034097012132406235,
-0.03330472856760025,
0.07704538106918335,
-0.02267785370349884,
-0.0214570052921772,
-0.026969512924551964,
-0.0033577964641153812,
0.02731768973171711,
-0.06116838380694389,
0.015069274231791496,
-0.08124963194131851,
-0.03941452130675316,
0.06216803193092346,
-0.025362662971019745,
-0.007223930209875107,
-0.04492946341633797,
-0.002050618873909116,
-0.031532660126686096,
-0.040710318833589554,
0.021229293197393417,
0.03767268359661102,
0.027718937024474144,
0.06386895477771759,
-0.06260927766561508,
0.010711741633713245,
-0.033973440527915955,
-0.018776196986436844,
0.015902061015367508,
0.0033878169488161802,
0.0007512135780416429,
-0.04198535904288292,
0.04090490564703941,
0.009535569697618484
] |
PMID:18652
|
Pathway of glucose catabolism in Caulobacter crescentus.
|
Glucose when present as a sole organic carbon source in a mineral salts medium is dissimilated by Caulobacter crescentus ATCC 15252 (strain CB-2) by the Entner-Doudoroff pathway throughout the culture cycle (exponential, transition, and stationary phase). Most of the available glucose that is present at the onset of exponential growth is assimilated by the cells during the transition phase or the period associated with stalk cell development. Swarmer cell development is minimized during this phase. During this same period the pH drops from 6.1 to 4.9 as a result of an abundant excretion of acetic acid. Simultaneously, poly-beta-hydroxybutyrate accumulates within the cells at an accelerated rate. An NADP-dependent glyceraldehyde-3-phosphate dehydrogenase is also present throughout the culture cycle which subsumes the presence of the subsequent enzymes of the Embden-Meyerhof-Parnas pathway in pyruvate formation. An operative tricarboxylic acid cycle is associated with cells throughout the culture cycle.
|
Pathway of glucose catabolism in Caulobacter crescentus. Glucose when present as a sole organic carbon source in a mineral salts medium is dissimilated by Caulobacter crescentus ATCC 15252 (strain CB-2) by the Entner-Doudoroff pathway throughout the culture cycle (exponential, transition, and stationary phase). Most of the available glucose that is present at the onset of exponential growth is assimilated by the cells during the transition phase or the period associated with stalk cell development. Swarmer cell development is minimized during this phase. During this same period the pH drops from 6.1 to 4.9 as a result of an abundant excretion of acetic acid. Simultaneously, poly-beta-hydroxybutyrate accumulates within the cells at an accelerated rate. An NADP-dependent glyceraldehyde-3-phosphate dehydrogenase is also present throughout the culture cycle which subsumes the presence of the subsequent enzymes of the Embden-Meyerhof-Parnas pathway in pyruvate formation. An operative tricarboxylic acid cycle is associated with cells throughout the culture cycle.
|
[
-0.06187768280506134,
0.028783094137907028,
-0.05274873226881027,
0.03530922532081604,
0.05104691535234451,
-0.009236915968358517,
-0.051836322993040085,
-0.008516918867826462,
-0.024022625759243965,
0.020162250846624374,
0.06389022618532181,
-0.08580254018306732,
0.025318879634141922,
0.03137926384806633,
-0.009654797613620758,
0.019805731251835823,
-0.04745909199118614,
0.042596831917762756,
0.002101754769682884,
-0.05000963434576988,
0.05703956261277199,
-0.034717172384262085,
0.010995283722877502,
-0.013945764862000942,
0.05145019292831421,
0.052062563598155975,
0.040076758712530136,
0.044960398226976395,
-0.07558398693799973,
-0.1637360006570816,
-0.0218877624720335,
0.02896164171397686,
0.05182844027876854,
-0.06051591411232948,
-0.0032924439292401075,
0.028298821300268173,
0.00625208206474781,
-0.005719967186450958,
-0.06885497272014618,
0.0996536985039711,
-0.026089994236826897,
0.0075443503446877,
-0.012292750179767609,
-0.00922246091067791,
-0.007957438938319683,
-0.03282895311713219,
-0.023164132609963417,
0.06751596182584763,
0.05057573318481445,
0.01796053536236286,
-0.0015549975214526057,
-0.021251622587442398,
0.03035508096218109,
-0.03366318717598915,
-0.04835446923971176,
-0.013800134882330894,
0.07391741871833801,
0.02709077298641205,
0.003462154185399413,
0.012226833030581474,
0.0336536169052124,
0.02566431276500225,
-0.12743023037910461,
0.028441784903407097,
0.05302412062883377,
-0.02480674535036087,
-0.054647792130708694,
-0.03995606675744057,
0.021160896867513657,
0.021606961265206337,
-0.047390833497047424,
-0.0013297873083502054,
0.03243182227015495,
0.002004871843382716,
0.028206659480929375,
-0.005854341667145491,
0.0368499681353569,
0.0290304534137249,
0.03119150549173355,
0.03675186634063721,
0.032547928392887115,
0.036659952253103256,
-0.004107315093278885,
0.016897480934858322,
-0.03932055085897446,
-0.031018029898405075,
0.05383940041065216,
-0.06408435106277466,
0.006064807530492544,
0.03369599208235741,
-0.010057881474494934,
-0.07211719453334808,
-0.05236676335334778,
0.04137232527136803,
-0.0718407854437828,
-0.023532304912805557,
0.028498506173491478,
-0.00019399577286094427,
0.06847521662712097,
0.35072553157806396,
-0.04736689105629921,
-0.018309708684682846,
0.03674224764108658,
0.057194896042346954,
0.001066152355633676,
-0.06619102507829666,
-0.01653861068189144,
0.014800691045820713,
0.01181984506547451,
-0.014861738309264183,
0.007336375303566456,
-0.03045565076172352,
0.01580546237528324,
-0.061024464666843414,
-0.004731937777251005,
0.004696196876466274,
-0.010681808926165104,
0.0149743827059865,
-0.05584747716784477,
0.020912224426865578,
0.018183263018727303,
0.031902607530355453,
-0.0013632611371576786,
0.025747576728463173,
0.035388946533203125,
0.026680096983909607,
-0.0005506206071004272,
0.09184905886650085,
0.04668193683028221,
-0.040504228323698044,
0.0875864177942276,
0.019421782344579697,
-0.02019914984703064,
0.02874901331961155,
-0.07595227658748627,
-0.027165232226252556,
0.06777743250131607,
-0.02562100440263748,
-0.02575787715613842,
-0.04331862926483154,
0.00853082537651062,
0.03322288393974304,
0.00683979457244277,
-0.0848911851644516,
-0.0225136186927557,
0.07662653923034668,
0.019032863900065422,
-0.0205801110714674,
-0.017965633422136307,
-0.007266214117407799,
0.02699441649019718,
0.030935266986489296,
0.05326927825808525,
0.016498135402798653,
0.0072846426628530025,
0.05074281990528107,
-0.0002803351962938905,
-0.0003115501021966338,
0.008686824701726437,
-0.026381036266684532,
-0.11264432221651077,
-0.028798798099160194,
0.038916461169719696,
0.1043223887681961,
0.026073243468999863,
-0.020303789526224136,
-0.009287594817578793,
0.0033685066737234592,
0.04193401709198952,
-0.01497066579759121,
0.01270732656121254,
-0.0030100117437541485,
-0.025251267477869987,
-0.05060308799147606,
0.013002848252654076,
0.0356331542134285,
0.030398409813642502,
0.02660074084997177,
0.009804162196815014,
0.02678232453763485,
0.04069848358631134,
0.01322084292769432,
0.005527565721422434,
0.04008941724896431,
0.04914242774248123,
-0.035986173897981644,
-0.040893930941820145,
-0.04807715117931366,
-0.053557973355054855,
0.03934379294514656,
-0.0628887265920639,
-0.03316484019160271,
-0.1002524197101593,
-0.0030078969430178404,
0.006469986401498318,
-0.03399014472961426,
0.001231829053722322,
-0.031520117074251175,
-0.00211306638084352,
-0.009496629238128662,
0.08482322841882706,
0.03812159597873688,
-0.013285334222018719,
0.06734912842512131,
0.009642727673053741,
-0.011903048492968082,
0.008989873342216015,
0.08092862367630005,
-0.037564948201179504,
0.04986781254410744,
-0.09497363120317459,
-0.03312322497367859,
-0.05612308904528618,
-0.03820738568902016,
-0.07575256377458572,
0.059940360486507416,
-0.017476018518209457,
0.03197696805000305,
0.03300662338733673,
0.03415532037615776,
-0.007129191420972347,
-0.036067117005586624,
-0.007373214233666658,
-0.3113889992237091,
0.018985934555530548,
0.004978843033313751,
-0.010029524564743042,
0.06830308586359024,
0.008952905423939228,
0.004610097035765648,
0.007533415220677853,
-0.043481770902872086,
-0.06076430529356003,
0.05341838672757149,
0.06197056174278259,
0.0005060700932517648,
-0.06096620485186577,
-0.012435339391231537,
0.017271677032113075,
0.054167963564395905,
-0.061827365309000015,
0.015667898580431938,
0.006795194931328297,
0.027082694694399834,
-0.0548812560737133,
0.09075070917606354,
0.005645249038934708,
0.03418170288205147,
-0.023591239005327225,
0.0528523325920105,
-0.02391497977077961,
0.06742988526821136,
-0.0556306354701519,
0.028389502316713333,
0.04500036686658859,
-0.01204258669167757,
-0.01960045099258423,
-0.05380755662918091,
-0.10015485435724258,
0.012391820549964905,
-0.08680382370948792,
0.05825376138091087,
0.0042943875305354595,
0.0065651265904307365,
0.007630243431776762,
-0.04230429604649544,
-0.040240272879600525,
0.005833268631249666,
-0.005663001444190741,
-0.048580847680568695,
-0.01011213380843401,
0.019415270537137985,
-0.042915623635053635,
0.032621532678604126,
-0.022988375276327133,
0.017869669944047928,
-0.031571172177791595,
0.028157219290733337,
-0.00885841529816389,
-0.010548083111643791,
0.03954228758811951,
-0.06173272430896759,
-0.002085538813844323,
-0.035553377121686935,
-0.003251593327149749,
-0.04662773385643959,
0.08198243379592896,
0.004326834809035063,
-0.0031670155003666878,
-0.03853911533951759,
-0.017856309190392494,
0.08526656031608582,
0.020941702648997307,
0.05495817959308624,
0.07884542644023895,
-0.0320335291326046,
-0.035894494503736496,
0.01229262538254261,
-0.008220670744776726,
-0.04726608842611313,
-0.03394092246890068,
-0.10651756078004837,
-0.01953832618892193,
-0.015356747433543205,
-0.0674930214881897,
0.11940968036651611,
-0.07314459979534149,
-0.002017493126913905,
0.003254757961258292,
0.0045594037510454655,
-0.009477449581027031,
0.009604144841432571,
0.038309626281261444,
0.024053100496530533,
-0.024771668016910553,
-0.07188820093870163,
-0.02796698547899723,
0.08140987157821655,
0.004691396839916706,
-0.2854103446006775,
0.023805668577551842,
0.027503840625286102,
-0.005727055482566357,
0.04268215224146843,
0.04167262092232704,
0.06007367745041847,
0.01028734166175127,
0.005258920602500439,
0.0004700651334132999,
0.020726870745420456,
0.022464707493782043,
0.05590847507119179,
0.024944057688117027,
0.045963071286678314,
0.041358694434165955,
0.0889299213886261,
-0.09946751594543457,
0.009984856471419334,
-0.03287719562649727,
-0.009855837561190128,
-0.055699944496154785,
0.09296908229589462,
0.05509321764111519,
0.02064223773777485,
-0.008983009494841099,
-0.029103996232151985,
0.028723426163196564,
-0.023373117670416832,
0.012505224905908108,
0.008269176818430424,
0.01872675120830536,
0.04964203014969826,
-0.014663422480225563,
0.047680098563432693,
0.02198261208832264,
0.029194004833698273,
-0.010235059075057507,
-0.015007947571575642,
0.012642201036214828,
-0.004824168514460325,
0.0009686909033916891,
-0.0840354710817337,
-0.053955260664224625,
0.0650712326169014,
-0.05179642513394356,
-0.02723723277449608,
-0.06582247465848923,
0.03231275454163551,
0.008527187630534172,
-0.05589120462536812,
-0.011608717031776905,
0.008475164882838726,
0.07196897268295288,
-0.022738372907042503,
0.04491748660802841,
-0.010590560734272003,
-0.05149956792593002,
-0.04553304612636566,
-0.034319501370191574,
0.020583685487508774,
-0.008630803786218166,
-0.021526703611016273,
0.07865707576274872,
-0.019825279712677002
] |
PMID:18656
|
Changes in blood pressure and antihypertensive treatment in a community over four years.
|
In 1971 persons aged 50 to 59 years in Albury were screened for hypertension and those with diastolic blood pressure equal to or over 110 mm Hg were notified. On screening of the sample in 1975, the proportion of persons receiving treatment had doubled to 22%. Most of those newly receiving treatment in 1975 had had diastolic blood pressures under 110 mm Hg in 1971. The mean systolic and diastolic pressures of the newly treated group had fallen by 13-7 and 9-1 mm Hg respectively to 153-5 and 91-6 mm Hg. The mean pressures of subjects already receiving treatment in 1971 and continuing it remained higher (163-1 mm Hg systolic and 100-9 mm Hg diastolic), despite their receiving relatively more medication. The latter group was composed of patients likely to have had more severe hypertension originally, and the importance of effective treatment for them is emphasized. The frequency with which subjects with mild hypertension are being treated demonstrates the urgent need to complete randomized controlled trials of treatment in this group.
|
Changes in blood pressure and antihypertensive treatment in a community over four years. In 1971 persons aged 50 to 59 years in Albury were screened for hypertension and those with diastolic blood pressure equal to or over 110 mm Hg were notified. On screening of the sample in 1975, the proportion of persons receiving treatment had doubled to 22%. Most of those newly receiving treatment in 1975 had had diastolic blood pressures under 110 mm Hg in 1971. The mean systolic and diastolic pressures of the newly treated group had fallen by 13-7 and 9-1 mm Hg respectively to 153-5 and 91-6 mm Hg. The mean pressures of subjects already receiving treatment in 1971 and continuing it remained higher (163-1 mm Hg systolic and 100-9 mm Hg diastolic), despite their receiving relatively more medication. The latter group was composed of patients likely to have had more severe hypertension originally, and the importance of effective treatment for them is emphasized. The frequency with which subjects with mild hypertension are being treated demonstrates the urgent need to complete randomized controlled trials of treatment in this group.
|
[
-0.04441319406032562,
0.03496827930212021,
-0.01911774091422558,
0.011037922464311123,
-0.006724042352288961,
0.020290620625019073,
0.004964963998645544,
0.010201454162597656,
-0.03414813056588173,
-0.04089583829045296,
0.0526282861828804,
-0.05018126219511032,
-0.02070150338113308,
-0.0045007034204900265,
-0.0726284459233284,
0.03112787753343582,
-0.014702271670103073,
-0.04349521920084953,
-0.013449205085635185,
0.05561218410730362,
-0.04468318447470665,
0.047747544944286346,
0.025546366348862648,
0.008225992321968079,
0.06325932592153549,
0.03743550553917885,
-0.025245219469070435,
-0.03453821316361427,
-0.06701801717281342,
-0.13277779519557953,
-0.005740239284932613,
0.02133265510201454,
0.03284735232591629,
-0.026558518409729004,
-0.03735021874308586,
0.029183872044086456,
-0.020152263343334198,
0.05561001971364021,
-0.011320525780320168,
0.044789280742406845,
-0.018044667318463326,
0.022769302129745483,
0.0056328983046114445,
0.003846077248454094,
-0.057703230530023575,
0.053504087030887604,
-0.02777635119855404,
-0.037436556071043015,
0.08795659244060516,
0.07822457700967789,
0.005187986418604851,
0.013188984245061874,
0.030100906267762184,
0.03964497894048691,
0.02487746998667717,
-0.0006551496917381883,
0.026691969484090805,
0.022738441824913025,
0.03826167806982994,
0.0029103730339556932,
-0.00932881236076355,
0.03492400795221329,
-0.17991182208061218,
0.029860297217965126,
0.030057711526751518,
0.03727958723902702,
-0.013790990225970745,
-0.04531952366232872,
0.013339871540665627,
-0.017491336911916733,
-0.023529887199401855,
0.04907173663377762,
-0.005871882662177086,
0.034507617354393005,
-0.03557012975215912,
-0.03343765810132027,
-0.03230426833033562,
-0.008273431099951267,
0.01833588257431984,
-0.06224818155169487,
0.06797190010547638,
0.006606221664696932,
-0.013546575792133808,
-0.04638028144836426,
0.009334016591310501,
-0.0908513069152832,
0.029864078387618065,
-0.005935720633715391,
0.0008892761543393135,
-0.028695134446024895,
-0.058595430105924606,
-0.012965686619281769,
-0.06637491285800934,
0.017013024538755417,
-0.010857174172997475,
-0.0026925259735435247,
-0.007430175319314003,
-0.023420589044690132,
-0.06346156448125839,
0.41739094257354736,
-0.0067730252631008625,
-0.012990553863346577,
-0.03868776932358742,
0.01990352012217045,
0.02786574698984623,
-0.030978290364146233,
0.01199555117636919,
0.024039417505264282,
0.07223471999168396,
0.049080800265073776,
0.014708232134580612,
0.015770619735121727,
0.15167246758937836,
0.0004137942160014063,
-0.00004443474972504191,
-0.031364791095256805,
-0.011972920969128609,
-0.0001497314078733325,
0.03187405318021774,
0.02711765095591545,
-0.03683757036924362,
-0.007911412976682186,
0.04256916791200638,
-0.034543219953775406,
0.04294608160853386,
0.05787959694862366,
-0.007323554717004299,
0.07860270887613297,
0.03841743245720863,
0.008178908377885818,
0.04634974151849747,
-0.029318083077669144,
-0.09418248385190964,
0.00918052438646555,
0.005902399774640799,
0.008377726189792156,
0.040325798094272614,
0.05382125452160835,
0.062120068818330765,
-0.021454235538840294,
-0.04954691603779793,
-0.0693419873714447,
-0.08549251407384872,
-0.0615033395588398,
-0.009146783500909805,
0.14907950162887573,
0.0028993068262934685,
0.05013243108987808,
-0.05157160386443138,
-0.009442063979804516,
-0.030485866591334343,
0.03928808867931366,
-0.0022360216826200485,
0.032725024968385696,
0.02216397412121296,
-0.00462527247145772,
0.042600639164447784,
0.05897507444024086,
0.0009034749818965793,
-0.007241895888000727,
-0.019058113917708397,
-0.02307048998773098,
-0.05424188822507858,
0.02047887071967125,
0.07527792453765869,
-0.021618349477648735,
-0.00930003821849823,
-0.01980370096862316,
0.008223626762628555,
0.07033301144838333,
0.06857708841562271,
0.004197949077934027,
0.06461141258478165,
0.023738712072372437,
0.04364263638854027,
-0.027464784681797028,
0.005286563653498888,
-0.018322907388210297,
0.02251862920820713,
-0.0240099485963583,
0.03317352384328842,
0.07594820111989975,
0.014830470085144043,
0.014671191573143005,
0.004409218672662973,
-0.003933299332857132,
-0.05727345868945122,
-0.0028460181783884764,
0.01496004220098257,
0.06462244689464569,
-0.026875078678131104,
-0.01727483421564102,
-0.05918022617697716,
0.022582929581403732,
0.019785922020673752,
-0.018213288858532906,
-0.04263298958539963,
0.008653511293232441,
-0.05790115147829056,
0.03308603912591934,
0.006977973505854607,
0.006963474676012993,
0.01723438687622547,
0.0280220415443182,
0.007202936336398125,
0.05764652416110039,
0.006772156804800034,
-0.02270243503153324,
0.05393694341182709,
-0.02016352489590645,
0.034788478165864944,
0.06520046293735504,
0.045031238347291946,
0.028011063113808632,
-0.01711265742778778,
-0.0034347043838351965,
-0.03810052573680878,
-0.020661894232034683,
0.0039906627498567104,
0.015178822912275791,
0.09049881249666214,
-0.04045630246400833,
-0.01683678664267063,
-0.32457810640335083,
-0.043083157390356064,
0.023463569581508636,
-0.03726620972156525,
0.03506183996796608,
-0.014258389361202717,
0.01160397008061409,
-0.03631816804409027,
-0.06264185160398483,
0.051545675843954086,
0.010520939715206623,
0.018963037058711052,
-0.04351936653256416,
-0.001601488678716123,
-0.027110088616609573,
-0.006504492834210396,
0.04169071465730667,
-0.018017565831542015,
0.009683302603662014,
-0.008624357171356678,
0.012266797013580799,
0.003908887971192598,
-0.06630181521177292,
0.005386916920542717,
0.004890406504273415,
-0.020957568660378456,
0.13706380128860474,
-0.054345350712537766,
-0.0849727913737297,
-0.011616644449532032,
-0.04478875547647476,
-0.01199684664607048,
0.006368530914187431,
-0.05661088600754738,
-0.0003657802881207317,
-0.04594289883971214,
-0.024869801476597786,
0.02262330986559391,
-0.05513696372509003,
-0.001887920661829412,
-0.03914443403482437,
0.006897916551679373,
-0.024318719282746315,
0.02565455064177513,
-0.07670387625694275,
-0.007013860624283552,
0.005765088368207216,
0.05187426134943962,
-0.04667414352297783,
-0.008518708869814873,
0.004740969743579626,
-0.0054321447387337685,
-0.014383750036358833,
-0.018072467297315598,
0.042331114411354065,
-0.017671288922429085,
-0.11185942590236664,
-0.01267218217253685,
-0.0075860521756112576,
-0.04256395995616913,
-0.04497238248586655,
0.012044673785567284,
0.043222811073064804,
0.0000664361214148812,
-0.04080845043063164,
-0.008063243702054024,
0.05255706608295441,
0.012723513878881931,
0.0016316177789121866,
0.056858129799366,
-0.025164300575852394,
0.0448773093521595,
-0.03156410530209541,
-0.00607184786349535,
0.05639638006687164,
0.02201627939939499,
-0.03698134422302246,
0.02591291442513466,
-0.009966781362891197,
-0.004968800116330385,
0.023740608245134354,
-0.09663307666778564,
0.032006584107875824,
0.06138366833329201,
-0.013153298757970333,
-0.04296225309371948,
0.0083090141415596,
0.02840917743742466,
-0.02565508335828781,
-0.0568346306681633,
-0.05584116280078888,
-0.03879421949386597,
-0.040243614464998245,
-0.021143851801753044,
0.016787560656666756,
-0.014566082507371902,
-0.28523963689804077,
0.02958591841161251,
0.005448734853416681,
0.006078188773244619,
-0.03542096167802811,
0.014142989180982113,
0.005598749965429306,
0.05006452649831772,
-0.03991430252790451,
0.02801833301782608,
-0.030468447133898735,
0.029606591910123825,
0.08168452978134155,
-0.014759520068764687,
0.0027235124725848436,
-0.046064455062150955,
0.00701138935983181,
-0.00834435224533081,
0.004234935622662306,
-0.017457567155361176,
0.020312253385782242,
-0.026758931577205658,
0.10300479829311371,
-0.00841009896248579,
-0.021706894040107727,
0.04721757024526596,
-0.020975925028324127,
0.04106517508625984,
0.0037792043294757605,
0.015396533533930779,
-0.04868606477975845,
0.07692167907953262,
0.0456964485347271,
-0.00361924571916461,
0.0735975131392479,
0.01319621317088604,
0.013070793822407722,
-0.054535359144210815,
0.01591205969452858,
0.004418389406055212,
0.023262016475200653,
-0.0077621713280677795,
-0.05818241462111473,
0.017853165045380592,
0.10972563922405243,
-0.023814262822270393,
0.009059611707925797,
-0.05377238616347313,
0.0319489948451519,
0.014232296496629715,
-0.03621688485145569,
-0.007800132967531681,
0.0815930962562561,
0.016715185716748238,
-0.011005057021975517,
-0.029423467814922333,
-0.03286580741405487,
-0.04548630118370056,
0.03372979164123535,
-0.007721641566604376,
-0.04863308370113373,
-0.013155956752598286,
0.005931150633841753,
0.042824022471904755,
0.04251564294099808
] |
PMID:18653
|
[Influence of amino acids on synthesis of extracellular proteases by Actinomyces thermovulgaris].
|
Various amino acids had different effect on the synthesis of exocellular proteases by Actinomyces thermovulgaris T-54. Glutamine and arginine induced the synthesis, the effect depending on their concentration in the medium. Other amino acids inhibited the synthesis of proteases. The synthesis of alkaline and neutral proteases was not coordinated, and the ratio between these enzymes was not constant in different variants of the experiment. The maximum synthesis of alkaline proteases was found before the formation of submerged spores.
|
[Influence of amino acids on synthesis of extracellular proteases by Actinomyces thermovulgaris]. Various amino acids had different effect on the synthesis of exocellular proteases by Actinomyces thermovulgaris T-54. Glutamine and arginine induced the synthesis, the effect depending on their concentration in the medium. Other amino acids inhibited the synthesis of proteases. The synthesis of alkaline and neutral proteases was not coordinated, and the ratio between these enzymes was not constant in different variants of the experiment. The maximum synthesis of alkaline proteases was found before the formation of submerged spores.
|
[
-0.045928843319416046,
0.02476441115140915,
0.006664684042334557,
0.003422162029892206,
-0.01360307913273573,
-0.022039104253053665,
0.01859908550977707,
0.009009987115859985,
-0.06731626391410828,
0.022435611113905907,
0.005199385341256857,
-0.032911501824855804,
0.018277475610375404,
0.0356898233294487,
-0.008720270358026028,
-0.015307973138988018,
-0.040957048535346985,
0.01830131933093071,
-0.09726805239915848,
-0.07468707114458084,
-0.05782833695411682,
-0.019228970631957054,
0.08225265890359879,
-0.058379415422677994,
0.051557209342718124,
0.040630947798490524,
0.010676125064492226,
0.06152723729610443,
-0.044990915805101395,
-0.18570441007614136,
0.0010186186991631985,
0.012304579839110374,
0.0347803570330143,
-0.05334607884287834,
-0.03244425728917122,
0.04859805852174759,
-0.023646829649806023,
-0.056958235800266266,
-0.04779941588640213,
0.052330132573843,
0.02254418283700943,
0.03746238350868225,
-0.012222654186189175,
-0.003975034225732088,
0.03026672452688217,
0.03112132102251053,
0.005824359133839607,
0.02077602781355381,
0.07651756703853607,
0.03323383629322052,
-0.004936307203024626,
-0.062105558812618256,
-0.01913955807685852,
0.030398501083254814,
0.029228080064058304,
-0.02825148217380047,
0.015696439892053604,
0.10327722132205963,
-0.05079347640275955,
-0.02100369520485401,
0.04275824874639511,
-0.0054671019315719604,
-0.14229510724544525,
0.037082355469465256,
0.04145057126879692,
0.03722967207431793,
0.0061710248701274395,
-0.025519372895359993,
0.03305516019463539,
0.05784394219517708,
-0.0751737430691719,
0.03289594128727913,
0.00964159145951271,
0.023600174114108086,
0.0776636153459549,
0.009730449877679348,
-0.04812787473201752,
0.01233458612114191,
0.05108165740966797,
-0.010540631599724293,
-0.002822419861331582,
-0.013061421923339367,
-0.010725646279752254,
0.010662185959517956,
-0.009750180877745152,
-0.032490070909261703,
-0.003659580834209919,
-0.006191558670252562,
0.06275542825460434,
0.010277625173330307,
-0.014831126667559147,
-0.0456799678504467,
0.03403973951935768,
0.0037620849907398224,
-0.1118079274892807,
0.006840769201517105,
0.00325708813033998,
-0.032994672656059265,
0.02278880774974823,
0.3649142384529114,
-0.03412846103310585,
-0.040440626442432404,
-0.03899165987968445,
0.015531007200479507,
-0.019985025748610497,
-0.002733967499807477,
-0.01584583893418312,
0.009016741067171097,
-0.03128821775317192,
0.04487297311425209,
0.11642225831747055,
0.04337455332279205,
-0.013258976861834526,
-0.017961926758289337,
-0.009885255247354507,
-0.005787234287708998,
0.10699064284563065,
0.023149192333221436,
-0.09581775963306427,
0.01531967706978321,
-0.04041125252842903,
-0.03769208863377571,
0.01943948119878769,
-0.0005889246822334826,
-0.0067054759711027145,
0.00962899997830391,
-0.051492176949977875,
0.10564595460891724,
0.06053002178668976,
-0.017902256920933723,
0.037902843207120895,
0.013386934995651245,
0.000737377442419529,
-0.009647690691053867,
0.0417361855506897,
-0.03685038536787033,
-0.000028267117158975452,
-0.0333133190870285,
0.03936241567134857,
0.022721951827406883,
-0.009574932977557182,
-0.032182879745960236,
-0.07964736223220825,
-0.03494562953710556,
0.01797647587954998,
0.057161517441272736,
0.012059983797371387,
0.015628909692168236,
-0.048895254731178284,
0.02835826389491558,
0.05343392491340637,
0.02917284145951271,
-0.021140025928616524,
0.02359701879322529,
0.027966251596808434,
0.0409647673368454,
-0.019193608313798904,
-0.012089408002793789,
-0.00608342420309782,
-0.03457687795162201,
-0.08365369588136673,
-0.034559305757284164,
0.022758545354008675,
0.048476170748472214,
0.04669443890452385,
0.028525665402412415,
-0.02396196313202381,
0.0013357866555452347,
-0.011679122224450111,
0.0025346833281219006,
-0.021432816982269287,
0.014025943353772163,
-0.04761775955557823,
-0.018547765910625458,
-0.027289215475320816,
0.015047979541122913,
0.00024577000294812024,
0.02002410776913166,
-0.01713559962809086,
0.04003346338868141,
0.060379933565855026,
0.02006317861378193,
0.05449099838733673,
-0.002513916464522481,
0.04362182691693306,
-0.08039107918739319,
-0.056897398084402084,
0.00763219827786088,
0.03729073703289032,
0.07578369975090027,
-0.040503960102796555,
0.02378416433930397,
-0.07015921920537949,
-0.021500088274478912,
-0.05787041410803795,
-0.05945412069559097,
0.011783504858613014,
0.05680037662386894,
-0.022352449595928192,
-0.05200172960758209,
0.09703566133975983,
0.035984791815280914,
-0.02216913178563118,
0.03787396103143692,
-0.018623873591423035,
0.009211337193846703,
-0.021431012079119682,
0.018427759408950806,
0.05653943493962288,
-0.027933016419410706,
-0.07829906046390533,
-0.01932501792907715,
-0.06688252836465836,
0.006375957280397415,
-0.07557748258113861,
0.061987295746803284,
-0.00396410608664155,
0.004156557843089104,
0.05319008603692055,
0.029885541647672653,
0.0009952737018465996,
-0.02254837565124035,
-0.020352214574813843,
-0.28516772389411926,
-0.007741243112832308,
-0.02547422982752323,
-0.042797185480594635,
0.07819113880395889,
-0.0659865289926529,
-0.004297104198485613,
0.00995228998363018,
-0.03684094548225403,
-0.01480440329760313,
0.07442257553339005,
0.06447725743055344,
-0.00597043614834547,
-0.03060789406299591,
0.0032918350771069527,
0.029438599944114685,
-0.005633181892335415,
-0.0500798262655735,
0.03720422834157944,
0.04398275911808014,
-0.041403673589229584,
-0.05469534918665886,
0.04442591592669487,
0.012857706286013126,
0.0073861004784703255,
-0.0014481450198218226,
0.0728100836277008,
0.004474234767258167,
0.010932148434221745,
-0.02560756541788578,
0.02280430682003498,
0.09050817042589188,
-0.03023618459701538,
-0.08904781937599182,
-0.03662337735295296,
-0.03860446810722351,
0.015710527077317238,
0.0003298513183835894,
0.021410582587122917,
-0.06563213467597961,
-0.026607103645801544,
0.043579451739788055,
-0.017499851062893867,
-0.03984352573752403,
-0.0037846534978598356,
-0.0651007667183876,
0.02260087989270687,
0.06359323114156723,
0.02151446044445038,
-0.040487468242645264,
0.013762437738478184,
0.05919545143842697,
0.054594848304986954,
-0.0205069649964571,
-0.03502264991402626,
-0.034872908145189285,
-0.05860339477658272,
0.00037109878030605614,
-0.017842432484030724,
0.046636875718832016,
0.0034571525175124407,
0.022026071324944496,
-0.013501268811523914,
0.03372107446193695,
-0.028801152482628822,
-0.005291289649903774,
-0.011973596177995205,
-0.03239016607403755,
0.043313104659318924,
0.018551725894212723,
0.06208909675478935,
0.11977705359458923,
-0.04261224716901779,
-0.020044609904289246,
0.01755867712199688,
-0.014309905469417572,
0.011017987504601479,
-0.047636616975069046,
-0.05646844580769539,
0.04172736778855324,
-0.03189210593700409,
-0.04566444084048271,
0.061531879007816315,
-0.05565842613577843,
0.007170638535171747,
0.06262775510549545,
-0.04086553305387497,
0.004182681441307068,
0.012257928028702736,
0.009969708509743214,
0.07889445126056671,
-0.030299268662929535,
-0.03548688068985939,
0.051903702318668365,
0.012078461237251759,
0.03391805291175842,
-0.23512724041938782,
0.021431593224406242,
0.06136154755949974,
-0.022110041230916977,
0.0020511718466877937,
0.0401027537882328,
-0.05111593380570412,
-0.0481673963367939,
-0.010240874253213406,
0.0745970606803894,
0.05202662944793701,
-0.03475043177604675,
0.07738452404737473,
0.03655248135328293,
0.013134877197444439,
0.03733453527092934,
0.050044070929288864,
-0.06596707552671432,
0.04986011981964111,
-0.01756436750292778,
-0.013344512321054935,
-0.0591680072247982,
0.1825265884399414,
-0.017306465655565262,
0.02200300060212612,
0.0841190442442894,
0.03002302721142769,
0.02536938712000847,
-0.06618214398622513,
0.059192460030317307,
0.012360736727714539,
0.047242406755685806,
0.05960121378302574,
-0.01705687679350376,
0.03198408707976341,
0.011915518902242184,
0.0019518476910889149,
-0.01761803962290287,
-0.08742184191942215,
0.027075471356511116,
-0.0026040514931082726,
-0.016502682119607925,
-0.07021617144346237,
-0.003361194860190153,
0.0562330037355423,
-0.02883313223719597,
-0.05652514472603798,
-0.05446980148553848,
-0.005366803612560034,
-0.005327916704118252,
-0.07009275257587433,
-0.03988678753376007,
-0.005294988863170147,
0.034160807728767395,
-0.01569260284304619,
-0.022109895944595337,
-0.05330664664506912,
0.0051267254166305065,
-0.04309067130088806,
0.04105903580784798,
0.05411774292588234,
-0.008185778744518757,
-0.042606182396411896,
0.009617059491574764,
0.015108210034668446
] |
PMID:18658
|
A comparison of the genetic and physical size of the streptomycin resistance locus in Pneumococcus.
|
Polyacrylamide gel electrophoresis of the 30S ribosomal proteins derived from six streptomycin resistant strains indicates that each mutation alters the same ribosomal protein (str-r protein). Preliminary data utilizing SDS gels indicates that the str-r protein has a molecular weight between 10,000 and 20,000 daltons. No significant differences could be detected between the molecular weight of the str-r protein when it is derived either from a sensitive or from a resistant strain, including those derived from strains carrying multisite mutations of different genetic size. We have estimated the size of the multisite str-r mutations to be less than 30 base pairs. Two factor crosses with str-r markers in the trans position demonstrate recombination frequencies expected of closely linked, intragenic markers although cotransfer frequencies, of these same markers from the cis position, are very low. It is concluded that the cotransfer frequencies represent a marker effect and possible explanations are discussed. A reinterpretation of the genetic map of the pneumococcal str-r locus is presented.
|
A comparison of the genetic and physical size of the streptomycin resistance locus in Pneumococcus. Polyacrylamide gel electrophoresis of the 30S ribosomal proteins derived from six streptomycin resistant strains indicates that each mutation alters the same ribosomal protein (str-r protein). Preliminary data utilizing SDS gels indicates that the str-r protein has a molecular weight between 10,000 and 20,000 daltons. No significant differences could be detected between the molecular weight of the str-r protein when it is derived either from a sensitive or from a resistant strain, including those derived from strains carrying multisite mutations of different genetic size. We have estimated the size of the multisite str-r mutations to be less than 30 base pairs. Two factor crosses with str-r markers in the trans position demonstrate recombination frequencies expected of closely linked, intragenic markers although cotransfer frequencies, of these same markers from the cis position, are very low. It is concluded that the cotransfer frequencies represent a marker effect and possible explanations are discussed. A reinterpretation of the genetic map of the pneumococcal str-r locus is presented.
|
[
-0.05699561536312103,
-0.03994014859199524,
-0.06414041668176651,
-0.004256462678313255,
0.03534015640616417,
0.0102990185841918,
-0.02700309455394745,
0.01610296033322811,
-0.017867818474769592,
0.038909535855054855,
0.013962122611701488,
-0.05734899640083313,
0.028746722266077995,
-0.02590855583548546,
-0.01304777804762125,
-0.038774117827415466,
-0.06448214501142502,
0.013165414333343506,
-0.032613109797239304,
-0.006979812402278185,
-0.00337966438382864,
0.025954285636544228,
0.039371591061353683,
-0.050922829657793045,
0.03799343854188919,
0.004530771169811487,
-0.013766998425126076,
-0.04398420453071594,
-0.04435104876756668,
-0.24466393887996674,
0.051611535251140594,
-0.009930205531418324,
0.007165583781898022,
-0.000973568472545594,
-0.018300315365195274,
-0.0051905266009271145,
0.029567023739218712,
-0.024561334401369095,
0.010571978986263275,
0.04192875325679779,
0.04167700558900833,
0.047874223440885544,
0.02424759231507778,
0.036575380712747574,
-0.009137083776295185,
-0.028942201286554337,
-0.054873306304216385,
0.02783113159239292,
0.08744509518146515,
0.025506282225251198,
-0.022431667894124985,
-0.031185666099190712,
-0.002851668046787381,
0.07594715058803558,
-0.02840258739888668,
-0.007042643614113331,
0.026836292818188667,
0.03500472381711006,
0.022408075630664825,
-0.05077863112092018,
-0.011696386151015759,
0.040414098650217056,
-0.1369352489709854,
0.012545442208647728,
0.03511439636349678,
0.03994502127170563,
0.010361772030591965,
-0.057666730135679245,
0.03120262734591961,
-0.011707480996847153,
-0.040139708667993546,
0.03512483462691307,
-0.033238913863897324,
0.061179399490356445,
0.014614611864089966,
0.0024749550502747297,
-0.05802130699157715,
-0.007082353811711073,
0.0798664465546608,
0.012852254323661327,
-0.0167082492262125,
0.016893429681658745,
-0.017187075689435005,
0.00826964434236288,
-0.06795140355825424,
-0.00006251773447729647,
0.009572441689670086,
-0.035643044859170914,
0.04809970036149025,
0.05884034186601639,
-0.02064056135714054,
-0.01165273878723383,
0.027128390967845917,
0.004740054719150066,
-0.07881275564432144,
0.007658586837351322,
0.02331632934510708,
0.03304002061486244,
0.02931019850075245,
0.4310470521450043,
0.0018071336671710014,
-0.035135943442583084,
-0.03825093060731888,
0.023139826953411102,
-0.0016658237436786294,
-0.007696786429733038,
0.02455129288136959,
-0.030162494629621506,
0.005726927891373634,
0.05569867789745331,
0.031996458768844604,
0.013721431605517864,
0.013612302020192146,
-0.013954409398138523,
0.022122783586382866,
-0.008607295341789722,
-0.023284321650862694,
0.03713523596525192,
-0.07016263902187347,
0.005393708124756813,
-0.07211281359195709,
-0.003685536328703165,
-0.007342490367591381,
-0.001050307648256421,
0.04271421208977699,
-0.007358242757618427,
-0.059975769370794296,
0.08293662965297699,
0.01855800859630108,
0.0032996453810483217,
0.011677979491651058,
0.025252584367990494,
-0.00924402941018343,
0.015944525599479675,
-0.022849544882774353,
-0.02880174294114113,
-0.024185270071029663,
-0.04184984043240547,
0.0487804114818573,
-0.009149313904345036,
-0.03422676771879196,
-0.09015006572008133,
-0.016868824139237404,
-0.008068621158599854,
-0.10441358387470245,
0.0903545394539833,
-0.06995067000389099,
0.06699501723051071,
-0.05391795560717583,
-0.009076062589883804,
0.06485901027917862,
0.092561736702919,
-0.006331452168524265,
-0.0524078793823719,
-0.010784321464598179,
0.07658381015062332,
-0.004287082701921463,
-0.024317311123013496,
0.01590641587972641,
-0.012908920645713806,
-0.04816466569900513,
-0.004344698507338762,
0.01971646025776863,
0.08096915483474731,
-0.017398852854967117,
-0.06227520480751991,
-0.0037452320102602243,
-0.008249837905168533,
0.018136490136384964,
-0.014324597083032131,
0.03623013570904732,
-0.011700503528118134,
-0.014117413200438023,
-0.041013311594724655,
-0.05250024050474167,
0.04732750728726387,
-0.048104606568813324,
-0.06363828480243683,
0.04660915583372116,
0.0482972115278244,
-0.0025440575554966927,
0.020100310444831848,
-0.048007573932409286,
-0.014113747514784336,
0.01640230044722557,
0.03654859960079193,
-0.07359807938337326,
0.02759242057800293,
-0.013034987263381481,
0.011289536021649837,
-0.005165322683751583,
0.02472120150923729,
-0.05015154555439949,
0.007786272093653679,
-0.0018063548486679792,
-0.04865343123674393,
-0.02937714383006096,
0.0258755162358284,
0.041341863572597504,
0.005844323430210352,
0.09600778669118881,
0.0531260222196579,
0.02166128158569336,
-0.004169944673776627,
0.0010269636986777186,
-0.0482756607234478,
0.03173070773482323,
-0.02493484504520893,
0.01963414065539837,
0.040121883153915405,
-0.030096760019659996,
-0.0075796376913785934,
-0.09186337143182755,
-0.006875517778098583,
-0.06699353456497192,
0.010525539517402649,
0.0187933798879385,
-0.0039945319294929504,
0.056153468787670135,
0.02095278911292553,
0.02092890813946724,
0.018216779455542564,
-0.038027044385671616,
-0.3122442364692688,
0.028069032356142998,
0.017107093706727028,
0.02349214069545269,
0.04343773424625397,
0.013850545510649681,
0.0010291782673448324,
0.0628369152545929,
0.028451552614569664,
-0.09530842304229736,
0.06821146607398987,
0.03686147183179855,
-0.06655114889144897,
-0.009391777217388153,
-0.027170078828930855,
0.014943369664251804,
-0.0105696776881814,
-0.018464667722582817,
-0.036225058138370514,
-0.015445182099938393,
0.013403482735157013,
-0.008375562727451324,
-0.010494835674762726,
0.0270537082105875,
0.04256395995616913,
-0.030634889379143715,
0.11678404361009598,
-0.02739819511771202,
0.05999009311199188,
0.06828127056360245,
0.021805167198181152,
-0.013943284749984741,
0.005686040502041578,
-0.022485895082354546,
-0.019459635019302368,
0.027610575780272484,
0.003482665168121457,
-0.007416596170514822,
0.025284098461270332,
0.007192567922174931,
0.019748689606785774,
0.03685108944773674,
-0.023630395531654358,
-0.059188149869441986,
-0.05403490364551544,
-0.010479322634637356,
0.026757923886179924,
0.008244562894105911,
0.016114048659801483,
0.06096675246953964,
-0.03890093415975571,
0.021551301702857018,
0.012239561416208744,
0.02704041637480259,
0.006018365267664194,
-0.01977158710360527,
-0.0022107355762273073,
-0.013626021333038807,
-0.014983903616666794,
-0.02793911285698414,
0.011733139865100384,
-0.019626036286354065,
0.009333490394055843,
-0.028169220313429832,
-0.009443622082471848,
-0.00602265028283,
0.003216620534658432,
-0.03564061224460602,
0.04098513722419739,
-0.00083783152513206,
-0.004693837836384773,
0.05958978459239006,
-0.04893346503376961,
-0.004917444661259651,
0.05846932530403137,
-0.005941752344369888,
-0.012782990001142025,
-0.020763404667377472,
-0.07135968655347824,
0.020609451457858086,
0.0055310288444161415,
-0.04276007041335106,
-0.004175808746367693,
0.003967771772295237,
0.03197969123721123,
-0.002553093945607543,
-0.0005113298539072275,
0.005645014811307192,
0.007710100617259741,
0.03440770506858826,
0.019275138154625893,
-0.06008005887269974,
0.030641986057162285,
-0.04779527336359024,
-0.01798839308321476,
0.031125158071517944,
-0.2980790436267853,
0.01980389468371868,
0.04660647734999657,
0.059297479689121246,
-0.025562157854437828,
0.07086346298456192,
0.0571666844189167,
-0.06255478411912918,
0.024944229051470757,
-0.026214133948087692,
0.055477164685726166,
0.025708401575684547,
0.07664136588573456,
0.05195564404129982,
0.0026233820244669914,
0.027510592713952065,
0.04479203000664711,
-0.03913050889968872,
0.08641975373029709,
0.02183300070464611,
0.015768764540553093,
-0.015701232478022575,
0.13213586807250977,
-0.011089433915913105,
0.01754310354590416,
-0.03951384499669075,
0.006544961128383875,
0.03180847316980362,
-0.004210708662867546,
0.01364306639879942,
0.01504100114107132,
-0.0026255487464368343,
0.07489483058452606,
-0.05008859932422638,
0.03903495892882347,
0.026518266648054123,
0.00892779603600502,
-0.05331401526927948,
-0.000289550400339067,
0.008720586076378822,
0.006213071756064892,
-0.026828963309526443,
-0.0750836431980133,
-0.03631844371557236,
0.10960835218429565,
-0.05202233046293259,
-0.010496941395103931,
-0.03255604952573776,
-0.006391783710569143,
-0.003903706092387438,
-0.022172844037413597,
0.014015561901032925,
-0.05283345654606819,
-0.016386965289711952,
0.004072562325745821,
-0.001919770846143365,
-0.037004195153713226,
-0.022676831111311913,
-0.008781395852565765,
-0.027735363692045212,
0.037934310734272,
-0.012367823161184788,
-0.03241705894470215,
0.05748683959245682,
0.013056286610662937
] |
PMID:18655
|
[Ecologic features of cultures of the species Clostridium gürfelii].
|
The Clostridium cultures decomposing pectin were isolated from flax straw during flax retting. The activity was manifested only at high pH values, the optimum pH being 6.7--8.2. Such ecological adaptation of the cultures is determined by the properties of the pectolytic enzyme complex which they synthesize. The complex does not contained polygalacturonase (the optimum pH 5.0) but has a high activity of pectate transeliminase (the optimum pH 8.0--8.4). The cultures differ from Clostridium felsineum which possesses a high activity of polygalacturonase and therefore can decompose pectin in acid medium. By their morphological, cultural and physiological properties, the bacteria are classed as Clostridium güfelii (Prévot, 1966).
|
[Ecologic features of cultures of the species Clostridium gürfelii]. The Clostridium cultures decomposing pectin were isolated from flax straw during flax retting. The activity was manifested only at high pH values, the optimum pH being 6.7--8.2. Such ecological adaptation of the cultures is determined by the properties of the pectolytic enzyme complex which they synthesize. The complex does not contained polygalacturonase (the optimum pH 5.0) but has a high activity of pectate transeliminase (the optimum pH 8.0--8.4). The cultures differ from Clostridium felsineum which possesses a high activity of polygalacturonase and therefore can decompose pectin in acid medium. By their morphological, cultural and physiological properties, the bacteria are classed as Clostridium güfelii (Prévot, 1966).
|
[
0.0038213212974369526,
0.02586514502763748,
0.007102744188159704,
0.044921327382326126,
0.08568674325942993,
-0.0744662806391716,
-0.02139030583202839,
0.053437769412994385,
-0.04866531491279602,
-0.015306966379284859,
0.02049793116748333,
-0.10375317186117172,
-0.027339491993188858,
-0.024599075317382812,
-0.051240235567092896,
-0.07625965029001236,
0.01471304427832365,
0.03423013165593147,
-0.0009016453986987472,
-0.025505466386675835,
0.052782315760850906,
0.003741733729839325,
-0.006724050268530846,
-0.03687369078397751,
0.03509531542658806,
-0.014438307844102383,
-0.009260629303753376,
-0.002726063597947359,
-0.02685956470668316,
-0.18839895725250244,
0.017730683088302612,
0.004927119705826044,
0.02543545328080654,
-0.02206767164170742,
-0.007709786295890808,
0.04743517190217972,
-0.008705729618668556,
-0.023084469139575958,
-0.029612623155117035,
0.026645179837942123,
0.04190923273563385,
0.05758502706885338,
0.006128543056547642,
-0.009825595654547215,
0.024121712893247604,
0.0002889271127060056,
0.018905743956565857,
0.019445030018687248,
0.018271638080477715,
-0.0037549715489149094,
-0.0013703699223697186,
-0.015068233013153076,
0.004005257040262222,
0.05225827544927597,
0.013989499770104885,
0.005519859027117491,
0.024950547143816948,
0.03445124626159668,
-0.022370629012584686,
0.025211254134774208,
0.023977160453796387,
0.0067771305330097675,
-0.11512476950883865,
0.02153177373111248,
0.028269832953810692,
-0.02364678867161274,
-0.023233473300933838,
-0.00924005825072527,
0.05384896695613861,
0.045316457748413086,
-0.08107469230890274,
0.0499967485666275,
-0.0013821030734106898,
0.020779067650437355,
0.055392734706401825,
0.01858067698776722,
-0.04970270395278931,
0.02589920535683632,
-0.025311579927802086,
0.04943504184484482,
0.025821682065725327,
0.03787466138601303,
-0.02875433675944805,
-0.008936230093240738,
0.0032704430632293224,
-0.04163619503378868,
0.05744636803865433,
-0.016966627910733223,
-0.010315551422536373,
0.07009187340736389,
0.03738350048661232,
-0.05498068407177925,
-0.022734155878424644,
-0.06553017348051071,
-0.05750184878706932,
0.003006580751389265,
0.06398985534906387,
-0.003810739377513528,
0.007081210147589445,
0.4123447835445404,
-0.07977047562599182,
-0.09205292165279388,
0.021928485482931137,
-0.010007253848016262,
0.021978134289383888,
-0.04331137612462044,
-0.014189308509230614,
0.007248006761074066,
0.0034728222526609898,
-0.011771531775593758,
0.04468672350049019,
0.023469893261790276,
-0.01362273097038269,
0.045778725296258926,
-0.0017472166800871491,
-0.02087830938398838,
0.007765490561723709,
0.06494677811861038,
-0.01997203193604946,
0.02951313927769661,
-0.019350579008460045,
-0.018295668065547943,
0.03450920805335045,
-0.06808392703533173,
0.04151196405291557,
0.02062893658876419,
-0.03745713457465172,
0.044127579778432846,
0.014197594486176968,
0.015695985406637192,
0.02198907546699047,
-0.003812667215242982,
0.02683519385755062,
-0.04039820283651352,
0.0024874142836779356,
-0.013121985830366611,
0.06941518187522888,
0.00825538206845522,
0.014517753385007381,
-0.01750662922859192,
-0.047654736787080765,
-0.014210156165063381,
-0.047334425151348114,
-0.07654894888401031,
-0.07074988633394241,
0.07147546857595444,
-0.04390639811754227,
0.02289147675037384,
-0.04790610074996948,
0.025501573458313942,
-0.04587365686893463,
0.037319716066122055,
-0.009771876037120819,
-0.0048833019100129604,
0.08468379825353622,
0.005563971120864153,
0.021969730034470558,
-0.04816292226314545,
0.024216296151280403,
-0.05751490592956543,
-0.042154405266046524,
0.02744917757809162,
0.02998313307762146,
0.09536360204219818,
0.060196198523044586,
0.0012356245424598455,
-0.040725186467170715,
0.010382900945842266,
0.012230067513883114,
-0.00423469441011548,
-0.0033065625466406345,
-0.0026658682618290186,
-0.030415426939725876,
-0.01806928589940071,
-0.017570793628692627,
0.013485380448400974,
0.021127672865986824,
-0.026493806391954422,
0.011683731339871883,
0.002296298509463668,
0.0377022884786129,
-0.021782703697681427,
0.011619674041867256,
0.030438628047704697,
0.025113461539149284,
-0.0028725587762892246,
-0.008880400098860264,
-0.007191299926489592,
-0.00422998983412981,
0.007449776865541935,
-0.038950420916080475,
-0.033610209822654724,
-0.026237323880195618,
0.05149853602051735,
-0.03536493703722954,
-0.03915747255086899,
0.005760866682976484,
0.06040911749005318,
0.011226825416088104,
-0.026560964062809944,
0.02950754389166832,
0.02393222786486149,
-0.00532104168087244,
0.01737542822957039,
-0.001332248211838305,
0.04712332412600517,
0.044940732419490814,
0.06449757516384125,
-0.04173032194375992,
-0.008772036992013454,
-0.017429959028959274,
0.026382597163319588,
-0.004830055870115757,
0.03203935921192169,
-0.07301493734121323,
0.07915791124105453,
0.023375283926725388,
-0.023998111486434937,
0.02350723370909691,
0.0339529849588871,
0.012529361993074417,
-0.07539471983909607,
-0.03252854570746422,
-0.30090609192848206,
0.0073790098540484905,
-0.018776630982756615,
-0.05059802159667015,
0.07254008948802948,
-0.04145780950784683,
-0.00944913923740387,
-0.028371503576636314,
0.035204797983169556,
-0.010320903733372688,
0.04336756840348244,
0.06400803476572037,
-0.04286569356918335,
0.036060232669115067,
0.06122913956642151,
0.05251169577240944,
0.031813304871320724,
-0.06495172530412674,
-0.006178929470479488,
-0.009500821121037006,
0.0362737812101841,
-0.08194950968027115,
0.03978124260902405,
0.03929613158106804,
-0.0042619649320840836,
-0.02311386913061142,
0.08887764066457748,
0.02557465434074402,
0.059479836374521255,
-0.02190132439136505,
-0.04377289488911629,
0.09793131798505783,
-0.06775397807359695,
-0.06790617853403091,
-0.02170700952410698,
0.014049218967556953,
0.0032330616377294064,
-0.04269450157880783,
0.013686807826161385,
0.0271940715610981,
0.028035327792167664,
0.0182204470038414,
-0.019165564328432083,
-0.10767301917076111,
-0.004062476567924023,
-0.03825109824538231,
-0.0050781965255737305,
0.07264547049999237,
0.00043215928599238396,
0.006209391634911299,
0.002981628989800811,
-0.03200022876262665,
0.04474296048283577,
0.04412449151277542,
-0.03484722226858139,
0.033649198710918427,
-0.027638426050543785,
0.08174096792936325,
-0.07674232870340347,
-0.0575454942882061,
-0.04400036856532097,
-0.030283553525805473,
-0.010689830407500267,
0.05562824010848999,
0.0009238471975550056,
-0.00002304085501236841,
-0.014442541636526585,
0.043038345873355865,
0.0315292589366436,
0.02804333157837391,
0.0020636161789298058,
0.06913335621356964,
-0.02864142693579197,
-0.027440126985311508,
0.016643429175019264,
0.05835079029202461,
-0.05412335321307182,
-0.023031488060951233,
-0.05941002070903778,
-0.03424734249711037,
0.040162138640880585,
0.05697250738739967,
0.06379000842571259,
-0.035045918077230453,
0.012480110861361027,
0.041184693574905396,
0.00034571310970932245,
-0.06983932852745056,
0.01916325092315674,
0.04031365364789963,
0.05861477926373482,
-0.013857610523700714,
-0.07750893384218216,
-0.01736392080783844,
0.05207666754722595,
0.021071288734674454,
-0.2601400911808014,
-0.057233262807130814,
0.09192152321338654,
0.004773195367306471,
0.0049238684587180614,
0.06423336267471313,
-0.005517110228538513,
-0.045336488634347916,
0.03869393840432167,
0.043871454894542694,
0.040608424693346024,
-0.03549192100763321,
0.035999249666929245,
0.02247660607099533,
0.025415539741516113,
0.044853437691926956,
-0.011576840654015541,
-0.04613229259848595,
0.006082372274249792,
-0.08467242121696472,
0.04050006344914436,
-0.061014898121356964,
0.08735234290361404,
0.011129960417747498,
0.03915659710764885,
0.014808625914156437,
-0.04240947961807251,
-0.0076982430182397366,
-0.046229757368564606,
0.03028225526213646,
0.03139987587928772,
0.021733129397034645,
0.06324765831232071,
-0.05378944054245949,
0.0347815677523613,
0.04066817834973335,
0.0023499787785112858,
-0.02939917892217636,
-0.04013719782233238,
-0.0033286719117313623,
-0.0010035225423052907,
-0.04204580560326576,
-0.10963156074285507,
-0.03539353236556053,
0.033141665160655975,
-0.1474425494670868,
-0.06723286211490631,
-0.06556311994791031,
-0.011258861981332302,
-0.0044751339592039585,
-0.060267433524131775,
-0.07125687599182129,
0.05579478293657303,
0.008664334192872047,
-0.01172687392681837,
0.025608418509364128,
0.00791905540972948,
-0.01685621589422226,
-0.006104067899286747,
-0.03907475993037224,
0.05311698466539383,
-0.026395143941044807,
-0.025429747998714447,
0.07120413333177567,
0.026318280026316643
] |
PMID:18664
|
Organization of purpine degradation in the liver of a teleost (carp; Cyprinus carpio L.). A study of its subcellular distribution.
|
Carp liver was fractionated by differential and density gradient centrifugation and assayed for enzymes of purine catabolism. While urate oxidase is an exclusively peroxisomal enzyme, only a very small percentage of the enzymes xanthine oxidase, allantoinase and allantoicase is associated with subcellular or ganelle fractions. There is no general purine catabolizing subcellular compartment. There is some but not yet conclusive evidence for the assumption that urate oxidase is a membrane bound enzyme.
|
Organization of purpine degradation in the liver of a teleost (carp; Cyprinus carpio L.). A study of its subcellular distribution. Carp liver was fractionated by differential and density gradient centrifugation and assayed for enzymes of purine catabolism. While urate oxidase is an exclusively peroxisomal enzyme, only a very small percentage of the enzymes xanthine oxidase, allantoinase and allantoicase is associated with subcellular or ganelle fractions. There is no general purine catabolizing subcellular compartment. There is some but not yet conclusive evidence for the assumption that urate oxidase is a membrane bound enzyme.
|
[
-0.03204863891005516,
-0.08267068862915039,
-0.014661175198853016,
-0.0026552164927124977,
0.05109195411205292,
-0.035716619342565536,
0.057003095746040344,
0.025044746696949005,
-0.027865460142493248,
-0.001346791977994144,
0.017966976389288902,
-0.13031762838363647,
0.030344808474183083,
-0.004193260800093412,
-0.01935182698071003,
-0.03664054349064827,
-0.017289532348513603,
0.05455031991004944,
-0.028990084305405617,
-0.020357949659228325,
0.06199212744832039,
-0.006809903308749199,
-0.019099095836281776,
-0.029209893196821213,
0.006475510541349649,
0.06168704479932785,
-0.026162389665842056,
-0.023072879761457443,
-0.04684880003333092,
-0.19483467936515808,
0.005645479075610638,
-0.01832512393593788,
0.02284029684960842,
-0.04202313721179962,
0.012554171495139599,
0.07651457190513611,
-0.04388536885380745,
-0.0509839802980423,
-0.04359733313322067,
0.06232466548681259,
0.010280543938279152,
0.035021621733903885,
-0.025471661239862442,
0.06817856431007385,
0.026819879189133644,
0.00989245530217886,
-0.034373242408037186,
0.008285781368613243,
0.07369565963745117,
-0.03524494916200638,
0.023342126980423927,
0.018034882843494415,
0.03461213409900665,
0.05550241097807884,
0.02234315127134323,
-0.017855137586593628,
0.07300213724374771,
0.06615682691335678,
-0.007278914097696543,
0.02859903872013092,
-0.024861585348844528,
0.018536197021603584,
-0.11631611734628677,
0.08793304115533829,
0.07012304663658142,
-0.06289546191692352,
-0.035532042384147644,
0.029183404520154,
0.016563966870307922,
0.007213400676846504,
-0.05352293327450752,
0.013550477102398872,
-0.022032804787158966,
0.02388511225581169,
0.010134350508451462,
-0.02572890743613243,
0.02925954945385456,
0.025871505960822105,
-0.00461360439658165,
0.029418030753731728,
0.020325127989053726,
-0.00609207060188055,
0.012875411659479141,
-0.011990243569016457,
0.002917851321399212,
-0.06511574983596802,
-0.0042518507689237595,
-0.03904043138027191,
-0.010808978229761124,
0.03528633341193199,
-0.06748705357313156,
-0.036685772240161896,
0.005175160709768534,
-0.006138186436146498,
-0.05867244303226471,
0.026820551604032516,
0.00126306910533458,
0.010752642527222633,
0.021088574081659317,
0.3866358697414398,
0.024476490914821625,
0.03139767795801163,
0.004612124525010586,
0.009601356461644173,
-0.008513686247169971,
-0.02557247132062912,
-0.03208538517355919,
-0.0032901933882385492,
-0.015785524621605873,
0.029428787529468536,
0.018689902499318123,
0.05674495920538902,
-0.02039634995162487,
-0.03742527589201927,
0.005120310001075268,
0.024896375834941864,
-0.03820143640041351,
0.024192461743950844,
-0.03127756714820862,
0.05252810940146446,
-0.05210236832499504,
-0.03663994371891022,
0.041659291833639145,
0.022334303706884384,
0.04321843385696411,
0.032081663608551025,
-0.014676399528980255,
0.061138857156038284,
0.0744716227054596,
0.0034522751811891794,
0.025823766365647316,
0.03056454285979271,
-0.02164546400308609,
0.01776387356221676,
-0.007199508138000965,
-0.03739713877439499,
0.08194702118635178,
-0.0978405699133873,
0.036800697445869446,
-0.022130252793431282,
-0.07141691446304321,
-0.08876305818557739,
-0.034379635006189346,
-0.057126644998788834,
-0.045480042695999146,
0.10170513391494751,
0.03321065381169319,
-0.016183141618967056,
0.020690836012363434,
-0.004972757305949926,
-0.010629544965922832,
0.07317957282066345,
-0.008654744364321232,
0.010688168928027153,
-0.006933404132723808,
-0.02504267916083336,
-0.040747907012701035,
-0.00661136768758297,
0.059964004904031754,
0.009344294667243958,
-0.08363635838031769,
-0.037486109882593155,
-0.016139015555381775,
0.07953290641307831,
0.04265538603067398,
-0.023321576416492462,
-0.060490503907203674,
-0.0003909794904757291,
0.027538323774933815,
0.02709341049194336,
-0.027730895206332207,
-0.012831770814955235,
0.002630692906677723,
-0.0035847576800733805,
-0.07673800736665726,
-0.005736736115068197,
0.04950118064880371,
0.03703978657722473,
0.012243729084730148,
-0.010011809878051281,
0.02569958195090294,
-0.017360473051667213,
-0.06446392834186554,
0.07175642251968384,
0.03500136360526085,
-0.02868317812681198,
-0.043858595192432404,
-0.02041265182197094,
-0.010029392316937447,
0.03827613592147827,
-0.03234037384390831,
0.021226292476058006,
-0.10371090471744537,
0.008098959922790527,
-0.0098196342587471,
-0.09294409304857254,
-0.0011737142922356725,
-0.001980200642719865,
0.004903578199446201,
-0.03338664770126343,
0.07801365107297897,
0.02605798840522766,
0.016460305079817772,
0.010692764073610306,
-0.01740202307701111,
0.012667978182435036,
-0.00028098802431486547,
0.04973984137177467,
0.014123569242656231,
0.04740482196211815,
-0.052181534469127655,
-0.0327649787068367,
-0.031866613775491714,
-0.009622314013540745,
-0.05120552331209183,
0.02920028753578663,
0.05890847370028496,
0.02361868880689144,
0.09354943037033081,
0.020409472286701202,
0.017011743038892746,
-0.09418494999408722,
-0.02486199513077736,
-0.2718709707260132,
-0.052806608378887177,
0.002354192780330777,
-0.048265304416418076,
0.012050439603626728,
-0.023821193724870682,
-0.01752144657075405,
-0.03751102089881897,
0.02503700740635395,
-0.021113881841301918,
-0.009264077059924603,
0.04413091763854027,
0.02778545394539833,
-0.019955139607191086,
0.0439206063747406,
0.07663046568632126,
0.04359300807118416,
-0.022279849275946617,
0.0313512422144413,
-0.000051163227908546105,
0.03647540137171745,
-0.05971787869930267,
0.0049703167751431465,
0.008649542927742004,
0.10554255545139313,
-0.011103012599050999,
0.11661417037248611,
0.010625732131302357,
0.007193287368863821,
0.003742141416296363,
-0.002122056670486927,
-0.0028333424124866724,
-0.04178192466497421,
-0.07514588534832001,
-0.03201849013566971,
0.000034583674278110266,
-0.05549168959259987,
-0.06657832115888596,
0.04915054515004158,
-0.0308429766446352,
-0.0653180181980133,
0.040867213159799576,
0.014020784758031368,
-0.032253723591566086,
-0.03770230710506439,
-0.03654741495847702,
-0.030778182670474052,
0.05695381388068199,
0.0363658182322979,
-0.023710288107395172,
0.08949887752532959,
-0.02230723947286606,
0.00136728142388165,
0.0011958294780924916,
-0.001525765284895897,
-0.017036255449056625,
-0.041951436549425125,
0.01564413495361805,
-0.006794239394366741,
0.0003397800901439041,
-0.017261004075407982,
-0.019860560074448586,
-0.04224668815732002,
0.03783107548952103,
-0.015505106188356876,
0.030168095603585243,
-0.01300881803035736,
-0.016221389174461365,
0.089334636926651,
0.017615381628274918,
0.007108911871910095,
0.07547582685947418,
-0.009071088396012783,
0.01473745983093977,
-0.017000725492835045,
0.0334332250058651,
-0.022058695554733276,
-0.015092626214027405,
-0.0056523531675338745,
-0.00358558539301157,
-0.017679424956440926,
-0.07158835232257843,
0.05807188153266907,
0.019928522408008575,
-0.012036049738526344,
0.05409625545144081,
0.011295820586383343,
-0.04413221403956413,
0.027826307341456413,
0.05671709403395653,
0.02998984418809414,
0.022978298366069794,
-0.060075849294662476,
-0.022022364661097527,
0.021016141399741173,
0.03019850328564644,
-0.25393494963645935,
0.02659735642373562,
0.01745956391096115,
-0.06831927597522736,
-0.015529606491327286,
0.013897452503442764,
0.03361573815345764,
0.004902307875454426,
-0.002227830933406949,
0.04736094921827316,
0.0746171846985817,
0.03374761715531349,
0.05367919057607651,
0.0565616637468338,
-0.031167956069111824,
0.05012821406126022,
0.061520837247371674,
-0.030660657212138176,
0.06807194650173187,
-0.045175950974226,
0.007889493368566036,
-0.06352744996547699,
0.1597888022661209,
-0.03719821572303772,
-0.02441704273223877,
0.014583168551325798,
-0.047035276889801025,
0.007589287124574184,
-0.032983630895614624,
0.003274303860962391,
-0.029978813603520393,
0.022493859753012657,
0.06995787471532822,
-0.0852072462439537,
-0.013374591246247292,
0.08149765431880951,
0.00026712872204370797,
0.018887100741267204,
-0.027066266164183617,
-0.0341239795088768,
-0.001145888352766633,
-0.052176423370838165,
-0.09764043241739273,
-0.05295129120349884,
0.05053907260298729,
-0.03737512230873108,
-0.040233150124549866,
-0.07989698648452759,
0.026797540485858917,
0.01197745930403471,
-0.022747287526726723,
-0.030968686565756798,
-0.027835538610816002,
0.02540101669728756,
0.013783963397145271,
0.004355484154075384,
-0.013220107182860374,
0.007767774164676666,
0.0036066866014152765,
0.0483282208442688,
-0.023519981652498245,
0.026901062577962875,
0.018172483891248703,
0.11045090109109879,
0.07321453094482422
] |
PMID:18665
|
Enzymatic synthesis of polyprenol monophosphate mannose in insects.
|
The microsomal fraction of insects was found to contain an enzyme which transfers mannose from guanosine diphosphate mannose to an endogenous or exogenous insect lipid and to other acceptors such as dolichol monophosphate or ficaprenol monophosphate. This activity depended on the presence of Triton X-100 and magnesium ions, the optimal concentration of the latter being 10mM. The optimal temperature of the reaction was 25 degrees C and the maximal activity was obtained at pH 7.9. The mannolipid formed behaved as a monophosphodiester when chromatographed on DEAE-cellulose. Weak acid treatment of the product liberated mannose. Its behaviour both on thin layer and Sephadex G-150 chromatography would indicate the presence of a number of isoprenyl units similar to the dolichol and different from the ficaprenol derivative. Stability to phenol treatment indicated that the lipid fraction of the mannolipid is an alpha-saturated polyprenol phosphate similar to dolichol monophosphate.
|
Enzymatic synthesis of polyprenol monophosphate mannose in insects. The microsomal fraction of insects was found to contain an enzyme which transfers mannose from guanosine diphosphate mannose to an endogenous or exogenous insect lipid and to other acceptors such as dolichol monophosphate or ficaprenol monophosphate. This activity depended on the presence of Triton X-100 and magnesium ions, the optimal concentration of the latter being 10mM. The optimal temperature of the reaction was 25 degrees C and the maximal activity was obtained at pH 7.9. The mannolipid formed behaved as a monophosphodiester when chromatographed on DEAE-cellulose. Weak acid treatment of the product liberated mannose. Its behaviour both on thin layer and Sephadex G-150 chromatography would indicate the presence of a number of isoprenyl units similar to the dolichol and different from the ficaprenol derivative. Stability to phenol treatment indicated that the lipid fraction of the mannolipid is an alpha-saturated polyprenol phosphate similar to dolichol monophosphate.
|
[
-0.05844390019774437,
-0.029215194284915924,
0.013619495555758476,
-0.007784663699567318,
-0.0007282641599886119,
-0.027891317382454872,
0.04791724309325218,
0.056351158767938614,
-0.08240414410829544,
-0.02090930938720703,
-0.012601799331605434,
-0.0654582753777504,
-0.007927410304546356,
0.015787815675139427,
-0.01567246951162815,
0.032155025750398636,
0.013128239661455154,
-0.00796265434473753,
-0.04977690801024437,
0.028353581205010414,
0.06399588286876678,
-0.009799176827073097,
0.04571474343538284,
-0.027279146015644073,
0.03623131290078163,
0.031278643757104874,
0.03085246868431568,
0.0010273533407598734,
-0.0049606007523834705,
-0.18307189643383026,
-0.01634959690272808,
-0.061818718910217285,
-0.0075021954253315926,
-0.04359424114227295,
-0.02653486840426922,
0.021592095494270325,
0.007430828642100096,
-0.07109390199184418,
-0.04701126366853714,
0.09228608012199402,
0.02387148328125477,
-0.005577549338340759,
-0.00973796471953392,
0.021179459989070892,
-0.04843449965119362,
-0.004317831713706255,
-0.04652513936161995,
0.0016659745015203953,
0.0756545439362526,
-0.06665762513875961,
-0.010366628877818584,
0.003773703705519438,
-0.04527091979980469,
0.027291754260659218,
0.006894469726830721,
-0.01705378293991089,
-0.019705194979906082,
-0.004182077944278717,
0.06662644445896149,
0.022518400102853775,
-0.01748456247150898,
0.016911599785089493,
-0.10738266259431839,
0.07589199393987656,
0.04177205264568329,
0.02229193039238453,
-0.054079264402389526,
0.028477204963564873,
0.020356418564915657,
0.03510812669992447,
-0.02336166799068451,
0.011556950397789478,
0.02516702003777027,
-0.020714379847049713,
-0.030536599457263947,
0.05132988095283508,
0.00840745959430933,
-0.058432627469301224,
0.013856889680027962,
0.05920550599694252,
-0.04623887315392494,
-0.026153674349188805,
-0.009936337359249592,
0.02331496588885784,
-0.040618717670440674,
0.022941356524825096,
0.029877809807658195,
0.020162733271718025,
0.06470418721437454,
0.005895302165299654,
0.015102367848157883,
-0.05533890426158905,
-0.018484795466065407,
-0.018622921779751778,
-0.10377811640501022,
0.09103047847747803,
0.024499403312802315,
0.0031117903999984264,
0.02461700700223446,
0.40493425726890564,
-0.02653917856514454,
0.050909191370010376,
0.03544275462627411,
-0.026507874950766563,
-0.0010901634814217687,
-0.04534733295440674,
-0.04219657555222511,
0.012377254664897919,
-0.009946663863956928,
0.04380230978131294,
0.07361506670713425,
0.04518581181764603,
0.07880071550607681,
0.008020317181944847,
-0.05426338315010071,
-0.05787336453795433,
-0.004103923682123423,
0.0036409294698387384,
-0.020867889747023582,
0.019439227879047394,
-0.022114524617791176,
0.005309917964041233,
-0.0055253272876143456,
0.017939046025276184,
0.026741739362478256,
0.03314093500375748,
-0.01590866968035698,
0.09030002355575562,
0.051124922931194305,
-0.0501086600124836,
0.019777612760663033,
0.06354808062314987,
-0.011784507893025875,
-0.008069328963756561,
0.00777069665491581,
-0.03127371147274971,
0.050963882356882095,
-0.003289218060672283,
0.028594721108675003,
-0.07451752573251724,
-0.03379403054714203,
-0.025938358157873154,
-0.039939772337675095,
-0.024714840576052666,
-0.03358204662799835,
0.08547501266002655,
0.05595996975898743,
0.06390412151813507,
0.004347226582467556,
0.0492110438644886,
-0.0005769295385107398,
0.03863346949219704,
-0.03517618402838707,
0.023288412019610405,
0.033530090004205704,
0.013491744175553322,
0.023678909987211227,
0.027110639959573746,
0.03486358001828194,
-0.032994646579027176,
-0.07577475905418396,
0.015672795474529266,
0.08823882788419724,
0.06593023240566254,
0.04619839787483215,
0.0029228099156171083,
-0.026882564648985863,
0.0180590208619833,
-0.014445729553699493,
-0.043937798589468,
0.016470855101943016,
-0.013241978362202644,
0.03320761397480965,
-0.058327414095401764,
-0.0416702926158905,
-0.019157687202095985,
0.030470125377178192,
-0.03661075234413147,
0.05297831445932388,
-0.048301540315151215,
0.03345344960689545,
-0.04496975988149643,
-0.034195054322481155,
0.002244591247290373,
0.006988718174397945,
-0.032139651477336884,
0.0018781303660944104,
0.010926182381808758,
-0.03154179826378822,
0.051208123564720154,
-0.013471121899783611,
0.02473078854382038,
-0.0006213627057150006,
-0.008560452610254288,
-0.020600929856300354,
-0.034607525914907455,
-0.009716346859931946,
0.00948818027973175,
-0.02716933935880661,
-0.011230112984776497,
0.08289694786071777,
0.040848422795534134,
0.02012467011809349,
0.028485935181379318,
-0.022825926542282104,
0.031174620613455772,
0.03532363846898079,
0.000332420167978853,
0.050891295075416565,
0.02649189904332161,
-0.028077207505702972,
-0.014277089387178421,
0.005022578407078981,
0.011469151824712753,
-0.06257221847772598,
0.0640244409441948,
-0.042375292629003525,
0.00623510405421257,
0.07102058082818985,
-0.008491480723023415,
-0.03764738515019417,
-0.0267674271017313,
-0.036012548953294754,
-0.2863406240940094,
-0.01795399934053421,
0.002251898404210806,
-0.02858617715537548,
0.11167018860578537,
0.007938430644571781,
0.011611138470470905,
-0.038256533443927765,
-0.01916777715086937,
-0.06557474285364151,
0.060450054705142975,
0.04653823748230934,
0.0036156235728412867,
0.03511449694633484,
-0.042960118502378464,
0.07472987473011017,
0.009371981024742126,
-0.02424011006951332,
0.01632152684032917,
0.042048875242471695,
-0.015830200165510178,
-0.08458530902862549,
-0.0019001023611053824,
-0.03780750930309296,
0.0631415992975235,
-0.09528592973947525,
0.08767873793840408,
0.05063633993268013,
0.014082507230341434,
-0.09458592534065247,
0.034961529076099396,
0.04742174223065376,
0.029357129707932472,
-0.08862350881099701,
-0.017563363537192345,
-0.023755373433232307,
0.007907356135547161,
-0.039836641401052475,
0.04589271545410156,
0.001056395354680717,
0.023789679631590843,
0.034590233117341995,
-0.011930642649531364,
-0.004878467880189419,
-0.015810709446668625,
-0.04784480854868889,
-0.011649142019450665,
-0.0009377200622111559,
-0.00952378660440445,
-0.033949803560972214,
0.014720123261213303,
0.024206655099987984,
0.05794374644756317,
-0.016680192202329636,
-0.015950409695506096,
-0.04332875460386276,
-0.028294870629906654,
-0.01962446980178356,
-0.06193050742149353,
0.016147790476679802,
0.01814638264477253,
-0.013571804389357567,
-0.03148939087986946,
0.015715982764959335,
0.014899239875376225,
0.00008857614739099517,
0.04072575643658638,
-0.04856058210134506,
0.06857340037822723,
0.03813176229596138,
-0.04571482539176941,
0.07166966050863266,
-0.0806376039981842,
0.022240374237298965,
0.02517160400748253,
0.03194635361433029,
-0.013692665845155716,
-0.010754990391433239,
-0.07383786886930466,
-0.021313121542334557,
-0.003455063793808222,
0.0027865373995155096,
0.03542193025350571,
-0.05148545280098915,
-0.04131069779396057,
0.052299853414297104,
-0.013652950525283813,
-0.030653953552246094,
0.04863741248846054,
0.006648106500506401,
0.00664704293012619,
-0.04638343304395676,
-0.0015153432032093406,
-0.04832204431295395,
0.05014626681804657,
0.03848622739315033,
-0.2759452164173126,
0.0037178397178649902,
-0.0030914656817913055,
0.01205987948924303,
-0.01640905626118183,
-0.00578281469643116,
0.05849100649356842,
-0.04739797115325928,
-0.009957220405340195,
0.0033548283390700817,
0.003273169044405222,
0.040977515280246735,
0.03975844010710716,
0.043852970004081726,
0.015485686250030994,
-0.021800769492983818,
-0.006653409916907549,
0.0023869043216109276,
0.02378135360777378,
-0.10064302384853363,
-0.022232981398701668,
-0.08279832452535629,
0.15886886417865753,
0.05066045746207237,
-0.02446701191365719,
0.05658412724733353,
0.016983062028884888,
0.0065618897788226604,
-0.09432797878980637,
0.023236192762851715,
-0.022883238270878792,
0.027424726635217667,
0.10170195996761322,
-0.017759662121534348,
0.000275909376796335,
0.08862125128507614,
-0.05623307451605797,
0.00027148862136527896,
0.012635398656129837,
0.05259350314736366,
-0.015454485081136227,
-0.05713646858930588,
-0.07835384458303452,
0.012003449723124504,
0.05331338942050934,
-0.043119050562381744,
-0.020672131329774857,
-0.02052432671189308,
-0.04946205019950867,
0.028071802109479904,
-0.014948153868317604,
-0.013683621771633625,
-0.04572432115674019,
0.0019474567379802465,
-0.02059774287045002,
-0.008254308253526688,
-0.04858899861574173,
0.033625803887844086,
-0.04301958158612251,
0.046502936631441116,
0.023251783102750778,
0.0013207937590777874,
-0.04375143349170685,
0.08248569816350937,
0.05420741066336632
] |
PMID:18666
|
A primer independent activity of rabbit muscle phosphorylase b.
|
Rabbit muscle phosphorylase b was found to be capable of forming protein bound alpha-1,4 glucosyl chains upon incubation of the enzyme with appropriate concentrations of glucose-1-phosphate with no primer addition (unprimed synthesis). This activity would only be present in a small fraction of the total muscle phosphorylase b activity, as judged from the high concentrations of enzyme which are required to demonstrate the occurrence of unprimed synthesis. Polyacrylamide gel electrophoresis shows the presence of a phosphorylase isoenzyme capable of accepting glucosyl moieties, giving rise to a glucosylated protein enzymatically active in the chain lengthening of its own glucan.
|
A primer independent activity of rabbit muscle phosphorylase b. Rabbit muscle phosphorylase b was found to be capable of forming protein bound alpha-1,4 glucosyl chains upon incubation of the enzyme with appropriate concentrations of glucose-1-phosphate with no primer addition (unprimed synthesis). This activity would only be present in a small fraction of the total muscle phosphorylase b activity, as judged from the high concentrations of enzyme which are required to demonstrate the occurrence of unprimed synthesis. Polyacrylamide gel electrophoresis shows the presence of a phosphorylase isoenzyme capable of accepting glucosyl moieties, giving rise to a glucosylated protein enzymatically active in the chain lengthening of its own glucan.
|
[
-0.08039262890815735,
-0.0960184633731842,
-0.005057763308286667,
0.0037944947835057974,
0.001154173631221056,
-0.054424483329057693,
0.002164318226277828,
0.017131267115473747,
-0.03178905323147774,
0.0017969770124182105,
0.008875585161149502,
-0.0775488018989563,
-0.04849826544523239,
0.04115171730518341,
-0.01495075598359108,
-0.030208038166165352,
0.001239411998540163,
-0.00512284180149436,
-0.06280546635389328,
-0.010419116355478764,
0.011455614119768143,
-0.019300371408462524,
0.003935881424695253,
-0.022875269874930382,
0.07374617457389832,
0.06869878619909286,
0.06420677155256271,
0.014603100717067719,
-0.030180560424923897,
-0.1964299976825714,
-0.012827873229980469,
-0.03534642234444618,
0.028377754613757133,
-0.04269605875015259,
-0.02079705335199833,
0.005561137571930885,
0.009199989959597588,
-0.012283694930374622,
0.003918320871889591,
0.043313801288604736,
0.04718804359436035,
-0.007725681643933058,
-0.009851878508925438,
0.053412921726703644,
0.07106607407331467,
-0.05818712338805199,
-0.057309139519929886,
0.02246027998626232,
0.03367077186703682,
0.020535137504339218,
-0.020663147792220116,
-0.04238240420818329,
0.012154574505984783,
0.009001746773719788,
-0.014359137043356895,
-0.012616933323442936,
0.012736808508634567,
0.035543616861104965,
0.02324596606194973,
0.023634793236851692,
0.017450764775276184,
0.08537151664495468,
-0.09686309099197388,
0.005598956253379583,
0.009180603548884392,
0.019906755536794662,
-0.013846787624061108,
-0.006537862587720156,
0.07237587124109268,
0.05601639673113823,
-0.034458234906196594,
0.060550615191459656,
0.05966423824429512,
0.00358431413769722,
-0.03039105236530304,
-0.03115285001695156,
0.007629804313182831,
0.04218168929219246,
0.03246083855628967,
0.09111109375953674,
-0.038724228739738464,
-0.015645863488316536,
0.05072101578116417,
0.005692651960998774,
-0.06462393701076508,
0.003778561484068632,
0.008395509794354439,
-0.03455742076039314,
0.04152695834636688,
0.020589355379343033,
-0.011296204291284084,
-0.08144045621156693,
-0.0516715832054615,
-0.01895732432603836,
-0.0908372700214386,
0.05937374010682106,
0.025897042825818062,
0.0024716444313526154,
-0.01774691417813301,
0.4086878299713135,
-0.07558931410312653,
0.029231607913970947,
-0.029621796682476997,
0.01338984351605177,
-0.01846461370587349,
-0.05112936347723007,
0.003532957285642624,
0.009597599506378174,
-0.03442971408367157,
0.01466654147952795,
0.11551837623119354,
0.0026887792628258467,
-0.02156941406428814,
-0.012911914847791195,
0.015767177566885948,
-0.016451779752969742,
-0.01997769996523857,
0.051761019974946976,
-0.026161521673202515,
0.04074271023273468,
-0.005330036859959364,
0.012365116737782955,
0.0008772728615440428,
-0.04260338842868805,
0.02556239441037178,
0.005559924058616161,
-0.02427365817129612,
0.09180650860071182,
0.07745259255170822,
-0.0047456021420657635,
0.0321711041033268,
0.021132247522473335,
0.016978049650788307,
-0.03448909893631935,
-0.02519141510128975,
-0.017623677849769592,
0.05891373008489609,
0.01032725814729929,
0.08863658457994461,
-0.055959783494472504,
-0.008369645103812218,
-0.01701662316918373,
-0.01845083013176918,
-0.01608278602361679,
-0.0053817532025277615,
0.08400176465511322,
0.028049221262335777,
0.03730710968375206,
-0.021424897015094757,
0.02861904539167881,
0.029018254950642586,
0.0388435460627079,
-0.03866881504654884,
0.014153915457427502,
-0.009620687924325466,
0.023938743397593498,
0.03657574951648712,
-0.03443334251642227,
0.06734973192214966,
-0.024001987650990486,
-0.1180286556482315,
-0.02881772816181183,
0.036824628710746765,
0.020495520904660225,
0.04036219045519829,
-0.027704410254955292,
-0.007678607013076544,
0.04820284619927406,
0.0021575074642896652,
-0.0013122917152941227,
0.0030660247430205345,
0.030587278306484222,
-0.0051559749990701675,
-0.021313592791557312,
-0.04554994776844978,
0.0004717393312603235,
-0.009356743656098843,
-0.009229598566889763,
0.01883520372211933,
0.03813294321298599,
0.04627734050154686,
-0.07442115992307663,
0.004059445112943649,
0.029409198090434074,
0.03809269890189171,
-0.01061064749956131,
-0.021537430584430695,
0.0011880731908604503,
0.030738282948732376,
0.08206663280725479,
-0.00908135436475277,
0.05362397059798241,
-0.10014623403549194,
-0.05050685256719589,
-0.011773972772061825,
-0.08216176182031631,
-0.01492753904312849,
0.006989670917391777,
-0.005096075125038624,
-0.010414308868348598,
0.07975352555513382,
0.013684535399079323,
0.041538625955581665,
0.03618873283267021,
-0.01984124816954136,
0.033659402281045914,
-0.008844020776450634,
0.04657569155097008,
0.016646433621644974,
0.03779975697398186,
-0.04765610024333,
-0.07885009050369263,
-0.06572272628545761,
-0.014623774215579033,
-0.06535167992115021,
-0.0013270379276946187,
-0.036089323461055756,
0.019080350175499916,
0.039300788193941116,
-0.014137579128146172,
-0.020450294017791748,
-0.028521161526441574,
-0.0333176925778389,
-0.2939038574695587,
-0.029204580932855606,
0.008487326093018055,
-0.03908419609069824,
0.029378607869148254,
-0.021795304492115974,
-0.0004949086578562856,
-0.01143383327871561,
-0.008197092451155186,
-0.09651298075914383,
0.0391002893447876,
0.019993051886558533,
-0.02571962960064411,
-0.02605990506708622,
-0.038853008300065994,
0.07325060665607452,
0.023696817457675934,
-0.0288557019084692,
0.016819868236780167,
-0.025954728946089745,
0.020766256377100945,
-0.011087831109762192,
0.053475894033908844,
-0.018990887328982353,
0.06495357304811478,
0.00524188531562686,
0.09537284821271896,
0.033711373805999756,
-0.014289213344454765,
-0.03141377121210098,
0.03998342528939247,
0.03588751703500748,
-0.04091775417327881,
-0.06845023483037949,
-0.040366608649492264,
0.0026675486005842686,
-0.012413205578923225,
-0.019547393545508385,
0.07927783578634262,
0.06300018727779388,
0.021328333765268326,
-0.01758640818297863,
-0.023486768826842308,
-0.03502033278346062,
0.005592572968453169,
0.04339929297566414,
0.025456739589571953,
0.016844594851136208,
0.024744540452957153,
-0.02598664164543152,
0.013768701814115047,
-0.030987072736024857,
0.07057502120733261,
-0.023026982322335243,
-0.04886162281036377,
-0.0236982274800539,
-0.010189362801611423,
0.032459527254104614,
-0.015399646945297718,
0.04233976826071739,
-0.00623505562543869,
-0.057408422231674194,
-0.05330490693449974,
0.055261775851249695,
0.04556773602962494,
0.004415844567120075,
-0.017836298793554306,
-0.010472186841070652,
-0.0016632755286991596,
0.005485892295837402,
0.020675072446465492,
0.06240677088499069,
0.019562993198633194,
0.04458700865507126,
0.029544316232204437,
0.0014199400320649147,
-0.05572083219885826,
-0.00947298388928175,
-0.1444375216960907,
-0.01072966493666172,
0.013542048633098602,
-0.02219090610742569,
0.08064261078834534,
-0.021018287166953087,
-0.024905497208237648,
-0.019838791340589523,
0.01242849975824356,
0.01405384112149477,
0.019418759271502495,
-0.005622305907309055,
0.01302929874509573,
0.0005185913178138435,
-0.0667835995554924,
-0.03555705398321152,
0.10563971102237701,
-0.02598278410732746,
-0.27646559476852417,
0.04409294202923775,
0.03457104042172432,
-0.025681883096694946,
0.01000584103167057,
0.04970652982592583,
0.04948623478412628,
-0.027992695569992065,
-0.04087713733315468,
0.008346689864993095,
0.04576072469353676,
0.009874332696199417,
0.04535394161939621,
0.014178883284330368,
-0.030549651011824608,
0.031082889065146446,
0.04721272736787796,
-0.034910351037979126,
0.048192285001277924,
-0.05635476112365723,
0.042156483978033066,
-0.03589333966374397,
0.14631341397762299,
0.04159305617213249,
0.0022519160993397236,
0.0595652274787426,
-0.016999626532197,
0.010492074303328991,
-0.024750906974077225,
-0.02096998132765293,
-0.03705606982111931,
0.035916518419981,
0.08192815631628036,
-0.05749101564288139,
-0.044650785624980927,
0.02755110338330269,
0.007642102427780628,
-0.030171753838658333,
0.02224939875304699,
0.027682755142450333,
-0.0019664668943732977,
-0.03470873832702637,
-0.05862909555435181,
-0.04580618441104889,
0.046832576394081116,
-0.018870564177632332,
-0.07931739091873169,
-0.048511803150177,
-0.051943715661764145,
-0.022434759885072708,
-0.060359060764312744,
-0.0272636990994215,
0.0001586613361723721,
0.022889554500579834,
0.02536342851817608,
-0.0263889841735363,
0.00478960620239377,
-0.019362865015864372,
0.016887959092855453,
0.026653451845049858,
0.05528580769896507,
-0.013755034655332565,
-0.029950859025120735,
0.05580940470099449,
0.0022834602277725935
] |
PMID:18667
|
Sucrose metabolism in green algae. I. The presence of sucrose synthetase and sucrose phosphate synthetase.
|
The presence of sucrose synthetase and sucrose phosphate synthetase has been demonstrated in two species of green algae: Chlorella vulgaris and Scenedesmus obliquus. Partial purification from crude extracts allowed the determination of the kinetic constants of algae enzymes. They are very similar to the ones reported for enzymes from higher plants.
|
Sucrose metabolism in green algae. I. The presence of sucrose synthetase and sucrose phosphate synthetase. The presence of sucrose synthetase and sucrose phosphate synthetase has been demonstrated in two species of green algae: Chlorella vulgaris and Scenedesmus obliquus. Partial purification from crude extracts allowed the determination of the kinetic constants of algae enzymes. They are very similar to the ones reported for enzymes from higher plants.
|
[
-0.10704335570335388,
-0.08587362617254257,
0.022663243114948273,
0.03323199599981308,
0.05871293321251869,
0.003005245001986623,
0.0025369212962687016,
0.014006402343511581,
-0.08689732849597931,
0.051720552146434784,
-0.006850368343293667,
-0.09516460448503494,
0.003350703977048397,
0.045898981392383575,
-0.026297787204384804,
-0.056566983461380005,
-0.015711655840277672,
0.04288175329566002,
-0.048684123903512955,
-0.0235736183822155,
0.10524632781744003,
-0.028286075219511986,
-0.007103343028575182,
-0.05911083146929741,
0.04313391447067261,
-0.005064643919467926,
0.05683576688170433,
0.00625826558098197,
-0.004453807137906551,
-0.16278348863124847,
-0.038068294525146484,
0.03124089539051056,
0.0018765149870887399,
-0.06678863614797592,
-0.0026601948775351048,
-0.002260081470012665,
-0.026404831558465958,
-0.009414651431143284,
-0.010940655134618282,
0.04409097135066986,
0.010046141222119331,
-0.0030655988957732916,
0.042032599449157715,
0.05898677557706833,
-0.01126685831695795,
-0.04854986071586609,
-0.025047944858670235,
0.03930914029479027,
0.02198079042136669,
-0.02195202000439167,
0.04146118089556694,
0.014155679382383823,
0.014026670716702938,
0.03875947743654251,
-0.04136601462960243,
-0.007726375944912434,
0.07749422639608383,
0.042259763926267624,
0.06935581564903259,
-0.02400522120296955,
0.012148723937571049,
0.05321674793958664,
-0.11664293706417084,
-0.03546992316842079,
0.037977248430252075,
-0.02521446719765663,
-0.03565608710050583,
-0.05702784284949303,
0.039196547120809555,
-0.030208859592676163,
-0.06796316802501678,
-0.020178474485874176,
0.01141327153891325,
0.003685998497530818,
0.022108737379312515,
-0.03543198108673096,
0.04129719361662865,
-0.00299878534860909,
0.006589454598724842,
0.06481117010116577,
0.033389266580343246,
-0.020937059074640274,
-0.004023371729999781,
0.002922136103734374,
-0.07597219198942184,
-0.06124810501933098,
0.05250062048435211,
0.047969505190849304,
0.06760337948799133,
0.0634535700082779,
-0.02308228425681591,
-0.12254032492637634,
0.02130068838596344,
-0.017510326579213142,
-0.08295176178216934,
0.027399420738220215,
0.03467734530568123,
0.050156887620687485,
0.04371416196227074,
0.3627656102180481,
-0.06366182863712311,
0.007434512954205275,
0.013987366110086441,
0.06208688020706177,
0.006547687109559774,
-0.03825286403298378,
-0.054853931069374084,
0.02320004440844059,
0.006968011148273945,
0.028925977647304535,
0.04760226979851723,
-0.007018788252025843,
-0.007262109313160181,
-0.036169327795505524,
-0.02130405604839325,
-0.016131257638335228,
0.010496249422430992,
-0.010633870027959347,
-0.05642838031053543,
0.022375505417585373,
-0.04051253944635391,
-0.039260320365428925,
0.06221485137939453,
-0.03560745716094971,
0.03923705220222473,
0.02744816243648529,
-0.007203724700957537,
0.045566461980342865,
0.06359899044036865,
-0.0016579177463427186,
0.06695505976676941,
0.037175022065639496,
0.003267676802352071,
0.005865675862878561,
-0.08469215780496597,
-0.019198568537831306,
0.1252669394016266,
-0.029352640733122826,
0.051573965698480606,
-0.038698676973581314,
-0.009407158009707928,
-0.07444451004266739,
0.01910867728292942,
-0.06576048582792282,
-0.027159862220287323,
0.07734540849924088,
0.04707101359963417,
0.08421532064676285,
-0.01792161911725998,
0.016564028337597847,
-0.026818236336112022,
0.07166017591953278,
-0.02503828890621662,
-0.003160910913720727,
-0.02616765722632408,
0.05254661291837692,
0.0009735578205436468,
0.024604501202702522,
0.06528880447149277,
-0.008594775572419167,
-0.08413476496934891,
0.053915686905384064,
0.007308237720280886,
0.035716742277145386,
0.04945832118391991,
-0.023060468956828117,
-0.014539685100317001,
0.026478398591279984,
-0.02629265747964382,
-0.0004862292262259871,
0.04988351836800575,
0.0757245346903801,
0.02228330634534359,
-0.0436396524310112,
-0.030980747193098068,
0.01761978678405285,
0.03977970778942108,
0.031342968344688416,
0.010173925198614597,
0.02449934184551239,
0.033313386142253876,
-0.023420928046107292,
0.0048849997110664845,
0.020014559850096703,
0.006122799590229988,
-0.04086291417479515,
0.029824240133166313,
0.014414768666028976,
-0.04076709598302841,
0.04158363491296768,
-0.034567855298519135,
-0.015439326874911785,
-0.03296374902129173,
0.008612439036369324,
-0.01478026993572712,
-0.04898284375667572,
0.05524145066738129,
-0.02125074341893196,
0.003524183528497815,
-0.01637354865670204,
0.04690251499414444,
0.02006608620285988,
0.022563021630048752,
0.035217009484767914,
0.04600893706083298,
-0.03203585371375084,
0.020251533016562462,
0.007165716029703617,
0.045062340795993805,
0.04191232845187187,
-0.039436060935258865,
-0.050187546759843826,
-0.016637638211250305,
0.04528891295194626,
-0.09620977938175201,
0.010668051429092884,
0.006898580119013786,
0.005282911006361246,
0.05868435651063919,
0.02256484143435955,
-0.045327454805374146,
-0.05605308338999748,
-0.09939281642436981,
-0.2721908390522003,
-0.0551595576107502,
-0.005601751152426004,
-0.0012725124834105372,
0.0926564484834671,
-0.004440052900463343,
-0.022245487198233604,
-0.004312420263886452,
-0.04915331304073334,
-0.032732319086790085,
-0.008914737030863762,
0.051140934228897095,
-0.00875401496887207,
-0.05324631929397583,
0.04890267178416252,
0.017857763916254044,
0.06167759746313095,
-0.05265795439481735,
0.061391569674015045,
-0.04936360567808151,
0.04597669094800949,
-0.0576026625931263,
0.05749830603599548,
0.0006270878366194665,
0.07519984245300293,
0.0034020710736513138,
0.07828176766633987,
0.017798738554120064,
0.008826970122754574,
-0.04847393557429314,
0.025745367631316185,
0.04642646014690399,
-0.005181275308132172,
-0.07322847098112106,
-0.00024082504387479275,
0.00462104007601738,
-0.028477462008595467,
-0.06317155808210373,
0.05418405681848526,
-0.008418038487434387,
0.017862314358353615,
0.03494042903184891,
-0.08210071921348572,
0.04482310637831688,
0.020254140719771385,
-0.03859077766537666,
0.0015808379976078868,
0.016399666666984558,
0.04773293063044548,
-0.018007928505539894,
-0.03451339900493622,
0.06918331980705261,
0.06637965142726898,
0.007523048669099808,
-0.014545313082635403,
0.027113275602459908,
-0.02477608248591423,
0.012971521355211735,
-0.022228611633181572,
-0.018493831157684326,
-0.01085988711565733,
-0.005856327246874571,
0.021215710788965225,
0.06241518259048462,
0.010946123860776424,
-0.005255337804555893,
-0.04458106309175491,
-0.01845075935125351,
0.017741410061717033,
0.028965512290596962,
0.014304250478744507,
0.02460162714123726,
-0.04069709777832031,
0.006523240357637405,
0.018132388591766357,
0.05383427441120148,
-0.028254060074687004,
-0.07572705298662186,
-0.05621088668704033,
-0.03641931712627411,
0.028814371675252914,
0.00013718014815822244,
0.14443457126617432,
-0.04294046759605408,
-0.00017469188605900854,
0.0010607747826725245,
-0.015884792432188988,
-0.05565303936600685,
0.016686657443642616,
-0.015198265202343464,
-0.015573441050946712,
-0.03712872415781021,
-0.06414645165205002,
-0.05201653391122818,
0.049516934901475906,
0.00697524705901742,
-0.25027137994766235,
0.003977627027779818,
0.029446376487612724,
-0.019343500956892967,
-0.011122053489089012,
-0.0014880114467814565,
0.02112044021487236,
0.026671167463064194,
0.01895144395530224,
0.018008336424827576,
0.0296305064111948,
0.035199664533138275,
0.023664474487304688,
0.06752172857522964,
0.003856444265693426,
0.07088392972946167,
0.05666785687208176,
-0.051511503756046295,
0.021680738776922226,
-0.06968777626752853,
-0.009204831905663013,
-0.04603111743927002,
0.10595768690109253,
0.01423974521458149,
0.00044798373710364103,
0.037598755210638046,
0.02181394211947918,
0.045391544699668884,
-0.05572124943137169,
0.05386453866958618,
0.001209421781823039,
0.040825873613357544,
0.06002701818943024,
-0.055182576179504395,
0.004553592298179865,
0.03313256427645683,
-0.009702255018055439,
-0.04282240942120552,
-0.0008686832152307034,
-0.0008975534583441913,
0.014915567822754383,
-0.027827728539705276,
-0.10033749788999557,
-0.035977259278297424,
0.09293211996555328,
-0.0836794376373291,
-0.02432139404118061,
-0.07067237049341202,
-0.04032564163208008,
-0.04415213316679001,
-0.006618695333600044,
-0.016601257026195526,
0.014532740227878094,
0.03442668169736862,
-0.018253643065690994,
-0.07064669579267502,
-0.06399237364530563,
-0.05682556331157684,
-0.041072290390729904,
-0.04654715582728386,
-0.020111605525016785,
0.005359954200685024,
-0.024425199255347252,
0.03407136723399162,
0.003820013254880905
] |
PMID:18668
|
Trisialoganglioside synthesis by a chicken brain sialyltransferase. Comparative study with the similar reaction for the synthesis of disialoganglioside.
|
An enzyme preparation from embryonic chicken brain catalyzes the transfer of sialic acid from CMP-N-acetylneuraminic acid to ceramide-Glc-Gal(NeuAc-NeuAc)-GalNAc-Gal (GDlb) to form ceramide-Glc-Gal(NeuAc-NeuAc)-GalNAc-Gal-NeuAc (GTlb). The sialyltransferase activity was measured during the development of the embryo, the subcellular distribution of this activity was determined and several kinetic properties of the reaction were examined. A comparative study with the similar reaction involved in the transfer of sialic acid to the terminal galactose in ceramide-Glc-Gal(NeuAc)-GalNAc-Gal (GMl) was made. The results obtained in this comparative study suggest that the transfer of sialic acid in both reactions is catalyzed by the same enzyme.
|
Trisialoganglioside synthesis by a chicken brain sialyltransferase. Comparative study with the similar reaction for the synthesis of disialoganglioside. An enzyme preparation from embryonic chicken brain catalyzes the transfer of sialic acid from CMP-N-acetylneuraminic acid to ceramide-Glc-Gal(NeuAc-NeuAc)-GalNAc-Gal (GDlb) to form ceramide-Glc-Gal(NeuAc-NeuAc)-GalNAc-Gal-NeuAc (GTlb). The sialyltransferase activity was measured during the development of the embryo, the subcellular distribution of this activity was determined and several kinetic properties of the reaction were examined. A comparative study with the similar reaction involved in the transfer of sialic acid to the terminal galactose in ceramide-Glc-Gal(NeuAc)-GalNAc-Gal (GMl) was made. The results obtained in this comparative study suggest that the transfer of sialic acid in both reactions is catalyzed by the same enzyme.
|
[
-0.032796744257211685,
-0.05512457340955734,
0.009485690854489803,
-0.012683979235589504,
-0.02498788572847843,
-0.0012937001883983612,
0.024779126048088074,
0.059424545615911484,
-0.0709586963057518,
-0.015431255102157593,
0.0468621551990509,
-0.10572588443756104,
-0.023452337831258774,
-0.005313041619956493,
-0.034299254417419434,
-0.008346650749444962,
-0.07344593852758408,
0.03184185549616814,
-0.047414381057024,
-0.0455392450094223,
0.014336840249598026,
-0.082534059882164,
-0.02706572599709034,
-0.0028223376721143723,
0.04386967420578003,
0.012666042894124985,
0.0183427631855011,
-0.016618922352790833,
-0.05072369799017906,
-0.1670682579278946,
-0.017547398805618286,
-0.011400260962545872,
0.00013273987860884517,
-0.05539461225271225,
-0.04037473350763321,
0.016481738537549973,
-0.0005798694328404963,
0.032677311450242996,
-0.06052197515964508,
0.08374837785959244,
0.05026571825146675,
0.02707747183740139,
-0.0009900005534291267,
0.00037190678995102644,
0.05428842827677727,
-0.03819296509027481,
-0.035337649285793304,
0.028061389923095703,
0.041907910257577896,
0.002666663145646453,
-0.00129634328186512,
-0.03111281245946884,
-0.01565275713801384,
0.045447103679180145,
0.00810463447123766,
0.011976842768490314,
0.0005585187464021146,
-0.0019977297633886337,
0.050474874675273895,
0.0008570425561629236,
-0.01448175311088562,
0.03527567535638809,
-0.15262414515018463,
0.05608457326889038,
-0.016564318910241127,
-0.009296235628426075,
-0.015684181824326515,
-0.04674212634563446,
0.02416597120463848,
0.08431284874677658,
-0.07483486086130142,
0.0095701077952981,
0.02364068664610386,
-0.004598303232342005,
0.018821746110916138,
0.006609821692109108,
0.001291368156671524,
0.05068138241767883,
0.0008455237257294357,
0.040005605667829514,
0.0008536805980838835,
0.054816797375679016,
0.013427029363811016,
-0.02810738794505596,
-0.07108784466981888,
0.0182507187128067,
-0.030601806938648224,
-0.005997898057103157,
0.03185366094112396,
0.05025216192007065,
-0.011539381928741932,
-0.07958745211362839,
-0.018794629722833633,
-0.021983420476317406,
-0.09098869562149048,
0.05686851590871811,
-0.009929842315614223,
0.00009900188888423145,
0.0032829674892127514,
0.35111913084983826,
-0.009549529291689396,
0.018037037923932076,
-0.012893429026007652,
0.007071053609251976,
0.026390068233013153,
-0.052028317004442215,
-0.04077579081058502,
-0.05193847790360451,
0.02867385372519493,
0.03559819608926773,
0.04723137617111206,
0.03989909589290619,
0.016402939334511757,
-0.060340285301208496,
-0.019620122388005257,
-0.020671173930168152,
0.024053480476140976,
-0.04143517091870308,
-0.028236746788024902,
0.05498037859797478,
-0.025724101811647415,
-0.011813378892838955,
0.00604415126144886,
-0.021940719336271286,
0.0560280941426754,
0.03841308876872063,
-0.08739352226257324,
0.06869108974933624,
0.08910202980041504,
0.0012003870215266943,
0.058923762291669846,
0.06652471423149109,
0.02754497155547142,
0.010832101106643677,
0.04133583605289459,
-0.01638488844037056,
0.0390850268304348,
-0.03937985748052597,
-0.062125325202941895,
-0.014026877470314503,
-0.06641345471143723,
-0.012864449061453342,
0.03153148293495178,
-0.021303247660398483,
0.0013132698368281126,
0.08611701428890228,
0.05933323875069618,
0.03666699305176735,
-0.01461189053952694,
0.06662922352552414,
0.043237920850515366,
0.10890240967273712,
0.007974534295499325,
-0.008135483600199223,
0.004369249101728201,
-0.03315271437168121,
-0.001412594923749566,
0.01859837956726551,
-0.009587893262505531,
-0.032419171184301376,
-0.02845033071935177,
-0.038690146058797836,
-0.02768014185130596,
0.09332909435033798,
0.058314453810453415,
-0.04170043766498566,
-0.029174920171499252,
0.05118041858077049,
0.0423225536942482,
-0.043831758201122284,
-0.00504653574898839,
0.04207167029380798,
0.0019178898073732853,
-0.08692488074302673,
-0.022704223170876503,
0.036921896040439606,
-0.009688000194728374,
0.0054160235449671745,
0.01300016138702631,
0.03214403614401817,
0.08695151656866074,
-0.03469513729214668,
-0.08051624149084091,
-0.01822027750313282,
-0.003602356417104602,
-0.05382382497191429,
0.01888744719326496,
0.005856830161064863,
-0.012485718354582787,
0.027337972074747086,
-0.03816121444106102,
0.015469621866941452,
-0.0348789356648922,
0.0028493867721408606,
-0.01836000196635723,
-0.05727598816156387,
0.00023838371271267533,
-0.028662383556365967,
0.02657490037381649,
-0.030324632301926613,
0.08029819279909134,
0.005278893746435642,
-0.006081727799028158,
0.06200436130166054,
0.02098778262734413,
-0.050743382424116135,
0.009044211357831955,
0.0009613974252715707,
0.014098185114562511,
0.05783724784851074,
0.024786466732621193,
-0.03823547437787056,
-0.06327089667320251,
0.01694394275546074,
-0.058915264904499054,
0.01650606282055378,
-0.04448726028203964,
0.059446848928928375,
0.10331150144338608,
-0.0006230436847545207,
0.026623912155628204,
-0.013568348251283169,
-0.06529662758111954,
-0.2998233139514923,
-0.04869740456342697,
0.014523373916745186,
-0.044452276080846786,
0.03861277922987938,
-0.05650146305561066,
0.024274153634905815,
-0.04788569360971451,
0.060363348573446274,
-0.027821585536003113,
0.10505282878875732,
0.05901472270488739,
-0.031590331345796585,
-0.013087814673781395,
-0.002620553830638528,
0.039893362671136856,
0.03204399719834328,
0.010902364738285542,
0.03180665895342827,
-0.009290150366723537,
0.019301731139421463,
-0.044829048216342926,
0.007159766275435686,
-0.04075535014271736,
0.021220877766609192,
-0.02765323966741562,
0.09439041465520859,
0.06243779510259628,
0.06115291640162468,
-0.026338309049606323,
-0.024461014196276665,
0.060377977788448334,
0.0449732169508934,
-0.06670638173818588,
-0.017517248168587685,
0.007388374302536249,
-0.009396648965775967,
-0.04200958088040352,
0.017221344634890556,
0.016844581812620163,
0.0018478953279554844,
-0.011005694977939129,
-0.03768415376543999,
-0.0747271180152893,
-0.05558674782514572,
0.04824577271938324,
-0.033595532178878784,
-0.02142312377691269,
0.01891184039413929,
-0.009948614984750748,
0.04294188693165779,
0.06411966681480408,
0.01057540811598301,
-0.038483742624521255,
-0.033137135207653046,
0.043054770678281784,
-0.012326385825872421,
0.0028818256687372923,
-0.05981900915503502,
0.05345231294631958,
-0.03391914814710617,
0.009104704484343529,
-0.0369788222014904,
0.02211167849600315,
-0.0005763860535807908,
0.06632237881422043,
0.018213914707303047,
-0.026463957503437996,
0.04727696254849434,
0.05405293405056,
0.015835929661989212,
0.09536221623420715,
-0.054486583918333054,
0.04784100502729416,
0.03805534169077873,
0.051603905856609344,
-0.00673443591222167,
-0.06070335581898689,
-0.07651340216398239,
0.02754073217511177,
-0.002855070633813739,
-0.05694136768579483,
0.06942698359489441,
0.0025938418693840504,
-0.030640391632914543,
0.0024954641703516245,
0.011061223223805428,
-0.040947798639535904,
0.049106284976005554,
-0.04511033743619919,
0.01141497865319252,
0.011956771835684776,
-0.0104517238214612,
-0.06127573549747467,
0.09243344515562057,
-0.020147006958723068,
-0.27092382311820984,
0.025076568126678467,
0.019911356270313263,
0.0009413863299414515,
0.02503637783229351,
-0.0294785313308239,
0.03491372615098953,
-0.030921263620257378,
-0.0397377610206604,
0.03741328418254852,
0.005304781720042229,
-0.014147993177175522,
0.043883077800273895,
0.029294166713953018,
0.007153486832976341,
-0.00590529665350914,
0.05820092558860779,
-0.00716659938916564,
0.035511333495378494,
-0.007882164791226387,
-0.03392470255494118,
-0.015389502048492432,
0.16804328560829163,
0.1055527999997139,
-0.019180091097950935,
0.002771876286715269,
-0.05184248834848404,
-0.006803787313401699,
0.0005391863523982465,
0.0014289292739704251,
0.00782736111432314,
0.00519610894843936,
0.04734168201684952,
-0.08375334739685059,
0.03043675608932972,
0.08331049978733063,
-0.08305352181196213,
-0.006444126833230257,
-0.007848411798477173,
0.058097612112760544,
0.008605148643255234,
0.01019238866865635,
-0.02093377523124218,
0.0038143829442560673,
0.10972980409860611,
-0.04584326595067978,
-0.03022121638059616,
-0.07315786927938461,
-0.03828509896993637,
-0.011329316534101963,
-0.03244230896234512,
-0.005611628293991089,
-0.01579306833446026,
-0.016878221184015274,
-0.027935141697525978,
0.015113509260118008,
0.01598367840051651,
0.017941901460289955,
-0.018322620540857315,
-0.004672694485634565,
-0.017917411401867867,
-0.019682323560118675,
-0.027854692190885544,
0.054131537675857544,
0.019068801775574684
] |
PMID:18669
|
Porphyrin biosynthesis. Immobilized enzymes. IV. Studies on aminolaevulate dehydratase attached to Sepharose.
|
Bovine liver aminolaevulate dehydratase (ALA-D) has been chemically attached to Sepharose 4B and its properties have been studied. The optimal conditions for coupling have been determined. It was found that the immobilized enzyme retained a significant percentage of the activity of the free enzyme. The coupling yield was rather high. The insolubilized enzyme requires both anaerobiosis and a thiol activator for maximal activity. It can be stored at 4 degrees C for long periods with little loss of activity and it can be repeatedly used without alteration of its enzymic capacity. Attachment of ALA-D to the gel has led to an enhanced thermal stability. pH optima of free and bound enzyme was the same while a small decrease in the Km of the matrix bonded ALA-D as compared to that of the soluble enzyme was observed. The use of the fixed-ALA-D for the preparation of PBG is described.
|
Porphyrin biosynthesis. Immobilized enzymes. IV. Studies on aminolaevulate dehydratase attached to Sepharose. Bovine liver aminolaevulate dehydratase (ALA-D) has been chemically attached to Sepharose 4B and its properties have been studied. The optimal conditions for coupling have been determined. It was found that the immobilized enzyme retained a significant percentage of the activity of the free enzyme. The coupling yield was rather high. The insolubilized enzyme requires both anaerobiosis and a thiol activator for maximal activity. It can be stored at 4 degrees C for long periods with little loss of activity and it can be repeatedly used without alteration of its enzymic capacity. Attachment of ALA-D to the gel has led to an enhanced thermal stability. pH optima of free and bound enzyme was the same while a small decrease in the Km of the matrix bonded ALA-D as compared to that of the soluble enzyme was observed. The use of the fixed-ALA-D for the preparation of PBG is described.
|
[
-0.10903941094875336,
0.028618894517421722,
-0.06625283509492874,
0.0069246138446033,
0.024308284744620323,
-0.09612572938203812,
0.04862875118851662,
0.0006664670072495937,
-0.07494980096817017,
-0.03304992988705635,
0.020170075818896294,
-0.07011833041906357,
0.00615563103929162,
-0.011963441967964172,
0.01675974391400814,
0.0060675558634102345,
0.0013804405461996794,
0.026938164606690407,
-0.04148511588573456,
-0.03292468935251236,
-0.0055687688291072845,
-0.011776616796851158,
-0.0030588130466639996,
-0.05421501398086548,
0.03825285658240318,
0.032367195934057236,
0.0211657527834177,
-0.006040363106876612,
-0.025006523355841637,
-0.17243395745754242,
0.05406023561954498,
-0.05145996809005737,
0.027628634124994278,
-0.040373533964157104,
-0.003708761651068926,
0.034954093396663666,
-0.039726730436086655,
0.004284620750695467,
-0.0030168003868311644,
0.013631323352456093,
0.04878057539463043,
-0.022700535133481026,
-0.030763281509280205,
0.049294859170913696,
-0.013527601025998592,
0.042684972286224365,
-0.027385588735342026,
0.02404302917420864,
0.0077376472763717175,
0.0239870548248291,
0.039692170917987823,
0.012972689233720303,
-0.016266684979200363,
0.04573974758386612,
0.03906399384140968,
0.020342674106359482,
0.007296369876712561,
0.06623058766126633,
-0.03238438814878464,
0.028663894161581993,
0.017478305846452713,
0.014639091677963734,
-0.07636337727308273,
0.07708194106817245,
0.02402324229478836,
-0.0114904148504138,
0.0045446595177054405,
-0.08633795380592346,
0.07590857893228531,
0.017148107290267944,
-0.0051881480030715466,
-0.007190464064478874,
0.019053883850574493,
-0.031084075570106506,
-0.006954164244234562,
0.03238225355744362,
0.07319553196430206,
-0.008844366297125816,
0.05592891201376915,
-0.012410703115165234,
0.002120604505762458,
0.009176451712846756,
-0.029493512585759163,
0.03738647326827049,
-0.006485276855528355,
0.04671206325292587,
0.04619564116001129,
-0.06316006928682327,
0.011881113052368164,
0.016126904636621475,
-0.018752997741103172,
-0.0629919171333313,
-0.04738517850637436,
-0.02287680096924305,
-0.12141823023557663,
0.053574442863464355,
0.0326068140566349,
0.02095092460513115,
0.006949024274945259,
0.3559643030166626,
-0.04781597480177879,
0.028508270159363747,
-0.03934841230511665,
-0.025571411475539207,
-0.06015926972031593,
0.006837456487119198,
-0.014219497330486774,
0.005121732596307993,
-0.06027785316109657,
0.026482606306672096,
0.06574548035860062,
0.05618062615394592,
0.06853396445512772,
-0.0004425521765369922,
-0.0062357536517083645,
0.04973478615283966,
-0.051324158906936646,
-0.027157479897141457,
-0.06673966348171234,
0.0622217021882534,
-0.04146270826458931,
0.028722699731588364,
0.05261168256402016,
0.051624543964862823,
0.041797664016485214,
0.017484569922089577,
-0.027795033529400826,
0.06089330092072487,
0.06043195724487305,
0.02339356206357479,
0.05564673990011215,
0.06073532998561859,
-0.03487502783536911,
-0.04005854204297066,
-0.027432912960648537,
-0.041249848902225494,
0.014691236428916454,
-0.03685342147946358,
0.09544143080711365,
0.02748941257596016,
-0.006667047273367643,
-0.015326599590480328,
-0.0037314235232770443,
-0.0494806282222271,
0.01360216923058033,
0.038395337760448456,
0.006186509970575571,
0.0270191989839077,
0.003622481832280755,
-0.005795241333544254,
-0.00032535585341975093,
0.030968287959694862,
-0.0038546628784388304,
0.04390903189778328,
0.0393332839012146,
0.028937725350260735,
-0.04192199930548668,
-0.01230977289378643,
0.00971448328346014,
0.02835927903652191,
-0.12246666103601456,
0.00017115531954914331,
0.020838303491473198,
0.06081961467862129,
0.0024655915331095457,
-0.013374502770602703,
-0.03946538269519806,
-0.01619637757539749,
0.02147689089179039,
0.011067375540733337,
0.016349278390407562,
0.04118502140045166,
-0.0070861950516700745,
-0.02993358112871647,
-0.025921862572431564,
0.04315158724784851,
0.014212371781468391,
0.043530501425266266,
0.032946474850177765,
-0.006891338154673576,
0.040797144174575806,
0.0009708623983897269,
-0.05804572254419327,
0.03663232922554016,
0.03295927122235298,
-0.04766273498535156,
-0.019883926957845688,
0.02941686473786831,
0.031852416694164276,
0.027767542749643326,
-0.004716344177722931,
0.051884256303310394,
-0.053763676434755325,
0.045909132808446884,
-0.05174121633172035,
-0.052322257310152054,
-0.0375007688999176,
0.017107123509049416,
0.03232072666287422,
-0.00045378654613159597,
0.10359826683998108,
0.023513009771704674,
-0.05159013718366623,
0.05992348864674568,
0.05904305353760719,
0.04625508189201355,
0.028082339093089104,
-0.0061882552690804005,
-0.0045281071215868,
-0.0008891173056326807,
-0.020831970497965813,
-0.015318365767598152,
-0.08970450609922409,
-0.04054756090044975,
-0.08921275287866592,
0.03689582645893097,
0.01661224663257599,
0.005298013333231211,
-0.00023049545416142792,
0.008998257108032703,
0.04163885861635208,
-0.07163581997156143,
0.02777945064008236,
-0.32849767804145813,
-0.01182172354310751,
-0.04584980383515358,
-0.026736324653029442,
0.04914738982915878,
-0.07066457718610764,
0.03171389549970627,
-0.042749710381031036,
-0.04849199205636978,
-0.00033874626387842,
0.053501538932323456,
0.011969950050115585,
0.02594910003244877,
-0.03478478267788887,
0.04449506103992462,
0.004424565937370062,
0.006704516708850861,
-0.0857229232788086,
-0.000053574214689433575,
-0.001341519644483924,
0.02322377637028694,
-0.021154120564460754,
0.0521579310297966,
0.0248505137860775,
0.07458474487066269,
-0.03556785732507706,
0.09721355140209198,
-0.0028700437396764755,
0.012128371745347977,
-0.010803625918924809,
0.01958410069346428,
0.06644922494888306,
-0.054692480713129044,
-0.08004562556743622,
-0.09287732094526291,
-0.022927306592464447,
0.00008874464401742443,
-0.07969813793897629,
0.05583357438445091,
-0.01169944740831852,
0.03954123333096504,
0.08587798476219177,
-0.029843751341104507,
-0.08997570723295212,
0.012505093589425087,
-0.007067510858178139,
-0.07588664442300797,
-0.01294571440666914,
-0.052446018904447556,
0.0023442599922418594,
0.03937475383281708,
-0.006101769860833883,
0.053257279098033905,
-0.02159895934164524,
-0.031247230246663094,
-0.001165191875770688,
-0.05176575481891632,
-0.009687517769634724,
-0.0515415258705616,
-0.01491557713598013,
-0.03103867918252945,
-0.0024312883615493774,
0.0033299680799245834,
0.04983745887875557,
-0.021550552919507027,
0.013456122018396854,
-0.05889520049095154,
0.010505818761885166,
0.028958113864064217,
0.025424130260944366,
0.021864939481019974,
0.05324450135231018,
-0.05786401033401489,
0.0315847285091877,
0.06697765737771988,
0.01481257751584053,
-0.044083647429943085,
-0.024102747440338135,
-0.07530545443296432,
0.02137075737118721,
-0.018126145005226135,
0.03148549050092697,
0.09996875375509262,
-0.044472530484199524,
0.007483895868062973,
0.04663003981113434,
0.03494086489081383,
-0.02614283189177513,
-0.009497467428445816,
0.0465555302798748,
0.07092887163162231,
-0.03301970660686493,
-0.025688903406262398,
0.011682501994073391,
0.04015858471393585,
0.00007571677269879729,
-0.26342645287513733,
-0.0269363671541214,
0.011633849702775478,
-0.06500409543514252,
-0.06554701179265976,
0.04365869238972664,
0.046111736446619034,
-0.03552458807826042,
0.006229150574654341,
0.017493337392807007,
-0.04026596248149872,
-0.004122019279748201,
0.06499683856964111,
0.03576916456222534,
0.03746848925948143,
0.02282137982547283,
0.026216886937618256,
-0.06275250017642975,
0.002392853843048215,
-0.0511338971555233,
-0.006480689160525799,
-0.025312796235084534,
0.14460766315460205,
-0.018143724650144577,
-0.03606865927577019,
0.062203921377658844,
0.029409023001790047,
0.027123333886265755,
-0.04193692281842232,
0.01216520182788372,
-0.020021609961986542,
0.036485668271780014,
0.08420401066541672,
-0.05913691595196724,
0.06134285777807236,
0.033739663660526276,
-0.015851614996790886,
-0.015912583097815514,
0.013114528730511665,
0.023134564980864525,
-0.012928695417940617,
-0.03337820619344711,
-0.07561865448951721,
-0.03969746455550194,
0.06145239993929863,
-0.011376423761248589,
-0.07465401291847229,
-0.020044254139065742,
-0.02234751358628273,
-0.0036287547554820776,
-0.03420209512114525,
-0.03231910616159439,
0.07441505789756775,
-0.015461338683962822,
-0.011795981787145138,
-0.028579939156770706,
0.015843452885746956,
0.015224895440042019,
-0.03604201599955559,
-0.004275259096175432,
0.020396685227751732,
-0.019158000126481056,
-0.04313644394278526,
0.0777561143040657,
0.0064600748009979725
] |
PMID:18670
|
[Complications of pertussis immunization (author transl)].
|
16 cases of neurological disease and/or death shortly after pertussis immunization are reported. Eight patients had convulsions, six with ensuing permanent defects. Severe polymyositis was observed in one case. Five infants died 12 h to 4 days after vaccination: two after acute encephalopathy and three in the form of a sudden unexpected death (SID). In two fatal cases the morphological changes in the brain corresponded to those of pertussis encephalopathy: neuronal degeneration in various parts of the cortex, especially in the region of the ammons horn, and in the cerebellum. There were no signs of inflammation. Three cases underwent forensic autopsy and death was attributed to bronchopulmonary infection. Complete neuropathological work-up was only done in one case, in which the brain was normal. The critique of episodical reports and the demand for prospective studies is appreciated. Knowledge of all possible forms of complications, however, is indispensable for future investigations. Polymyositis and SID have so far not been listed as abnormal reactions to immunization. The majority of our cases became known accidentally from hospital sheets or from discussions with collegues. For a detection of all possible cases a greater awareness of doctors for the problem of pertussis immunization appears necessary. Only another 23 cases have been reported to the health authorities of the state of Lower Saxony during the last 6 to 7 years. Of those, nine were either harmless reactions or diseases probably unrelated to vaccination. Two were cases of SID, 12 and 72 h after vaccination. It is concluded that only a minor proportion of possible complications is presently reported to the health authorities.
|
[Complications of pertussis immunization (author transl)]. 16 cases of neurological disease and/or death shortly after pertussis immunization are reported. Eight patients had convulsions, six with ensuing permanent defects. Severe polymyositis was observed in one case. Five infants died 12 h to 4 days after vaccination: two after acute encephalopathy and three in the form of a sudden unexpected death (SID). In two fatal cases the morphological changes in the brain corresponded to those of pertussis encephalopathy: neuronal degeneration in various parts of the cortex, especially in the region of the ammons horn, and in the cerebellum. There were no signs of inflammation. Three cases underwent forensic autopsy and death was attributed to bronchopulmonary infection. Complete neuropathological work-up was only done in one case, in which the brain was normal. The critique of episodical reports and the demand for prospective studies is appreciated. Knowledge of all possible forms of complications, however, is indispensable for future investigations. Polymyositis and SID have so far not been listed as abnormal reactions to immunization. The majority of our cases became known accidentally from hospital sheets or from discussions with collegues. For a detection of all possible cases a greater awareness of doctors for the problem of pertussis immunization appears necessary. Only another 23 cases have been reported to the health authorities of the state of Lower Saxony during the last 6 to 7 years. Of those, nine were either harmless reactions or diseases probably unrelated to vaccination. Two were cases of SID, 12 and 72 h after vaccination. It is concluded that only a minor proportion of possible complications is presently reported to the health authorities.
|
[
-0.044299107044935226,
-0.01476197224110365,
-0.011649597436189651,
-0.01414603553712368,
-0.005322963465005159,
-0.022820476442575455,
0.07570800930261612,
0.045237231999635696,
-0.03341229632496834,
-0.01439665537327528,
-0.01662973128259182,
-0.054050058126449585,
-0.010207210667431355,
-0.012062889523804188,
-0.0012396262027323246,
-0.005799078848212957,
-0.049194175750017166,
-0.0528135746717453,
-0.05190004035830498,
0.022643715143203735,
-0.09133588522672653,
0.04120079055428505,
-0.02181803062558174,
-0.04234464839100838,
-0.008482067845761776,
0.008027249947190285,
0.028342578560113907,
-0.006384704727679491,
-0.028668390586972237,
-0.16624921560287476,
-0.0133731160312891,
-0.07190608978271484,
-0.0705651268362999,
-0.06100945919752121,
-0.04668011516332626,
0.03774631768465042,
0.01408471167087555,
0.06854619830846786,
0.001311293337494135,
0.03730526939034462,
0.03377944603562355,
0.07287853211164474,
0.00922281201928854,
-0.004598327446728945,
-0.013689320534467697,
-0.014427798800170422,
-0.022971566766500473,
-0.03907376155257225,
0.14219921827316284,
0.007221120409667492,
-0.06172285974025726,
-0.04953218251466751,
0.08103968948125839,
0.10681085288524628,
-0.017853768542408943,
-0.032437145709991455,
0.012979363091289997,
-0.00359216146171093,
0.025891859084367752,
0.06289564818143845,
-0.018389282748103142,
0.02139044739305973,
-0.12487483769655228,
0.04568830132484436,
0.005413508508354425,
-0.024065274745225906,
-0.03994913026690483,
-0.054927948862314224,
0.006360827479511499,
0.06095170974731445,
-0.026230227202177048,
0.08375336974859238,
0.02457122504711151,
0.01810397207736969,
-0.015323379077017307,
0.017352838069200516,
0.0030564945191144943,
-0.042868901044130325,
0.032770786434412,
0.05007103458046913,
-0.008117604069411755,
0.04440063238143921,
-0.016226673498749733,
-0.06053418666124344,
0.022282546386122704,
0.0005786475958302617,
0.022034842520952225,
-0.04539895057678223,
-0.019540857523679733,
0.031700700521469116,
0.025149332359433174,
-0.035425376147031784,
-0.032504431903362274,
0.00697273900732398,
-0.05785810574889183,
0.005366809666156769,
-0.0001704589230939746,
0.00733831524848938,
-0.050070688128471375,
0.3927355706691742,
0.005733910948038101,
-0.035537634044885635,
0.008855106309056282,
-0.003416594350710511,
-0.010873367078602314,
-0.008367909118533134,
-0.004119499120861292,
-0.04705618694424629,
-0.02566995471715927,
0.04269793629646301,
-0.007511747535318136,
0.03237179294228554,
0.02472248673439026,
0.039274830371141434,
0.0008992704679258168,
0.04346216097474098,
0.050468578934669495,
-0.008768786676228046,
-0.021490318700671196,
0.03816448524594307,
0.020760517567396164,
-0.041569776833057404,
0.034814707934856415,
-0.058148231357336044,
0.06882334500551224,
0.0005192792159505188,
0.009477101266384125,
0.11527490615844727,
0.035852961242198944,
0.004952404648065567,
0.10064835846424103,
0.011177152395248413,
-0.007127870339900255,
0.02564062550663948,
0.019922956824302673,
-0.021700628101825714,
0.038521960377693176,
-0.00924358144402504,
0.03286461904644966,
-0.04412132501602173,
-0.05857240781188011,
-0.0467568077147007,
0.0019432015251368284,
-0.07186111062765121,
-0.03175148367881775,
0.03096175380051136,
-0.004877193830907345,
-0.04683460667729378,
-0.006838623899966478,
-0.00225507072173059,
-0.017225975170731544,
0.07743409276008606,
-0.0395851694047451,
0.016630228608846664,
0.006743771489709616,
-0.015054862946271896,
0.011207963339984417,
0.03635681793093681,
0.025499798357486725,
-0.04139299318194389,
0.0026510893367230892,
-0.07731612026691437,
0.019658558070659637,
0.08947109431028366,
0.00707244873046875,
0.03296889737248421,
-0.0009651203290559351,
-0.05760656297206879,
0.02477964386343956,
-0.06889291852712631,
0.022948142141103745,
0.04067451134324074,
0.05960668995976448,
-0.030061187222599983,
0.01694563962519169,
0.0056009963154792786,
-0.010403327643871307,
-0.02615828439593315,
-0.014277719892561436,
0.036990147083997726,
-0.0028416679706424475,
0.011887525208294392,
0.007198254577815533,
0.030416984111070633,
-0.030103743076324463,
-0.018058357760310173,
-0.016559334471821785,
0.03959915041923523,
0.07218830287456512,
-0.0060361046344041824,
0.03602476045489311,
-0.06296632438898087,
-0.11217333376407623,
-0.04177900403738022,
0.030668826773762703,
-0.00893532857298851,
-0.05542460456490517,
-0.0078110471367836,
0.061797674745321274,
-0.03365859016776085,
0.042463406920433044,
-0.02368748188018799,
-0.035910867154598236,
0.025126885622739792,
0.005451899487525225,
0.026696693152189255,
-0.02510835975408554,
-0.004138869233429432,
0.05937865749001503,
-0.004363423213362694,
-0.005088524427264929,
-0.0028645589482039213,
0.0064231762662529945,
-0.023729553446173668,
-0.002612764947116375,
0.016531499102711678,
-0.07502897828817368,
0.06303039938211441,
0.027251049876213074,
0.031075872480869293,
0.031212218105793,
0.022869139909744263,
0.013708535581827164,
-0.2838023900985718,
-0.08012933284044266,
-0.029330488294363022,
-0.06797333806753159,
-0.03220820054411888,
-0.03023580275475979,
-0.05786580964922905,
0.010737950913608074,
0.030559320002794266,
-0.019667169079184532,
0.04858582466840744,
0.024312684312462807,
-0.05321710184216499,
-0.028239857405424118,
0.003716250415891409,
0.06255669891834259,
0.03325439989566803,
-0.04274895042181015,
-0.020734230056405067,
-0.037077996879816055,
-0.049983613193035126,
0.010263051837682724,
0.009914027526974678,
0.023822113871574402,
-0.009843026287853718,
-0.03684079647064209,
0.14679376780986786,
0.03835705667734146,
0.03136700391769409,
-0.03437710180878639,
0.011788742616772652,
0.07484452426433563,
0.039073843508958817,
-0.04979627579450607,
0.05922149866819382,
0.002468500752002001,
-0.049743711948394775,
0.05432772636413574,
-0.07322163879871368,
-0.012446874752640724,
-0.08127949386835098,
0.018920110538601875,
0.012879665940999985,
-0.023913757875561714,
-0.016618218272924423,
0.0068899234756827354,
-0.029231030493974686,
-0.005691148806363344,
0.0266871340572834,
0.047635097056627274,
0.018491389229893684,
-0.06052442640066147,
0.008634371683001518,
0.023017019033432007,
0.058875784277915955,
-0.06502990424633026,
-0.025106893852353096,
0.01012301817536354,
-0.08617822080850601,
0.03742428869009018,
0.032439760863780975,
-0.006926065776497126,
-0.010287068784236908,
-0.08169452100992203,
-0.03928811475634575,
0.06314411014318466,
0.06267157942056656,
-0.00576613936573267,
0.03214714303612709,
0.015958569943904877,
-0.04199477285146713,
0.13929015398025513,
-0.03176334500312805,
-0.08344273269176483,
0.07674597203731537,
0.04073940962553024,
-0.0172145813703537,
-0.0016894262516871095,
-0.019954722374677658,
0.03807174041867256,
0.02183021977543831,
-0.0028432609979063272,
-0.02592388167977333,
0.043277472257614136,
0.0009786855662241578,
0.028496308252215385,
-0.05447359010577202,
-0.01531894039362669,
-0.045490674674510956,
-0.02097168192267418,
0.024972932413220406,
-0.00818328931927681,
-0.036555975675582886,
0.01800752989947796,
-0.007421729154884815,
0.030794907361268997,
-0.22320209443569183,
0.00410345708951354,
0.0563901849091053,
-0.021614108234643936,
0.04574121534824371,
0.010678078979253769,
-0.08576223254203796,
-0.011201473884284496,
-0.031095756217837334,
-0.031437620520591736,
0.055442411452531815,
0.0024281051009893417,
0.07381568104028702,
0.008181682787835598,
-0.04059679061174393,
0.03408107906579971,
-0.03716696798801422,
0.000017827353076427244,
0.04505181685090065,
-0.034080326557159424,
0.04378877952694893,
0.0028770240023732185,
0.13138501346111298,
0.0323321595788002,
-0.04293087124824524,
-0.015335606411099434,
-0.0015651772264391184,
0.09944143891334534,
-0.005793034099042416,
0.0463235042989254,
0.029290486127138138,
-0.07288907468318939,
0.049668192863464355,
0.028734492138028145,
0.08380211889743805,
-0.03250354900956154,
-0.033046621829271317,
0.03330643102526665,
0.03551597148180008,
0.02075548656284809,
0.006047823466360569,
-0.007835768163204193,
0.05830134078860283,
-0.010848994366824627,
0.05954023823142052,
0.04919387027621269,
-0.015598620288074017,
-0.05762539431452751,
-0.02293786220252514,
0.039349377155303955,
-0.04003604128956795,
-0.06037331372499466,
0.026370754465460777,
0.0019426037324592471,
-0.013527080416679382,
-0.05108330026268959,
0.06347476691007614,
-0.023014439269900322,
0.010432588867843151,
-0.021285077556967735,
0.031085435301065445,
-0.010764732956886292,
-0.09656742215156555,
0.06241590529680252,
0.036118146032094955
] |
PMID:18672
|
Amelioration of metabolic acidosis with fludrocortisone therapy in hyporeninemic hypoaldosteronism.
|
In four patients with renal hyperchloremic acidosis and hyperkalemia, hyporeninemic hypoaldosteronism and chronic renal insufficiency (glomerular filtration rates of 13, 31, 35 and 44 ml per minute per 1.73 m2), prolonged administration of fludrocortisone increased urinary potassium and net acid excretion, corrected hyperkalemia and substantially ameliorated acidosis. Except in the patient with the lowest glomerular filtration rate, the increased net acid excretion was due mostly to increased ammonium excretion. Urine pH decreased initially in each patient, but in the three patients with the highest filtration rates, it increased subsequently as ammonium excretion increased, indicating that renal ammonia production increased. Urinary ammonium excretion correlated inversely with serum potassium concentration and did not decrease on discontinuation of therapy if hyperkalemia was prevented from recurring. In patients with renal acidosis and hyporeninemic hypoaldosteronism, administration of mineralocorticoid hormone can augment both renal hydrogen-ion secretion and, by correction of hyperkalemia, renal ammonia production, and thereby ameliorate metabolic acidosis.
|
Amelioration of metabolic acidosis with fludrocortisone therapy in hyporeninemic hypoaldosteronism. In four patients with renal hyperchloremic acidosis and hyperkalemia, hyporeninemic hypoaldosteronism and chronic renal insufficiency (glomerular filtration rates of 13, 31, 35 and 44 ml per minute per 1.73 m2), prolonged administration of fludrocortisone increased urinary potassium and net acid excretion, corrected hyperkalemia and substantially ameliorated acidosis. Except in the patient with the lowest glomerular filtration rate, the increased net acid excretion was due mostly to increased ammonium excretion. Urine pH decreased initially in each patient, but in the three patients with the highest filtration rates, it increased subsequently as ammonium excretion increased, indicating that renal ammonia production increased. Urinary ammonium excretion correlated inversely with serum potassium concentration and did not decrease on discontinuation of therapy if hyperkalemia was prevented from recurring. In patients with renal acidosis and hyporeninemic hypoaldosteronism, administration of mineralocorticoid hormone can augment both renal hydrogen-ion secretion and, by correction of hyperkalemia, renal ammonia production, and thereby ameliorate metabolic acidosis.
|
[
-0.001333712600171566,
-0.0021545260678976774,
0.03051968291401863,
0.07363267987966537,
-0.005432400852441788,
-0.043569598346948624,
0.039705757051706314,
0.03175521641969681,
-0.01712028868496418,
-0.03940322622656822,
-0.00811992771923542,
-0.07799097895622253,
0.0787009745836258,
0.054300133138895035,
0.009570886380970478,
0.03472796455025673,
0.025871172547340393,
0.012542190961539745,
-0.08398518711328506,
-0.014330027624964714,
0.02852839231491089,
0.0025827374774962664,
0.04311210289597511,
0.011779289692640305,
0.007810174487531185,
-0.04793287813663483,
0.02061130851507187,
-0.008639931678771973,
-0.04563349485397339,
-0.16740942001342773,
-0.0061765918508172035,
0.0004831268161069602,
0.023555032908916473,
-0.030738133937120438,
-0.05486432835459709,
0.004963023588061333,
-0.0488065741956234,
0.0038973395712673664,
-0.02094930037856102,
0.07257211953401566,
0.022689558565616608,
0.0321783609688282,
-0.008969579823315144,
0.023178737610578537,
-0.03885538876056671,
0.029391968622803688,
-0.030364802107214928,
0.01326698251068592,
0.11978226155042648,
0.05520264804363251,
0.0025572143495082855,
-0.028721783310174942,
0.03626058250665665,
0.06518147140741348,
0.03169010579586029,
-0.03633417561650276,
0.013837394304573536,
0.0648646280169487,
-0.02181539684534073,
0.0094739506021142,
-0.004123338032513857,
0.03732344135642052,
-0.16167929768562317,
0.03660888597369194,
0.05028688162565231,
0.0016696397215127945,
-0.05159517377614975,
-0.04881516098976135,
0.038134291768074036,
0.0142940953373909,
-0.05239921808242798,
0.024316269904375076,
-0.015465821139514446,
0.010818050242960453,
0.019096432253718376,
0.006046748254448175,
-0.01769990473985672,
-0.015196392312645912,
-0.00912455003708601,
-0.01679825596511364,
0.013723972253501415,
0.04546825587749481,
-0.04315028339624405,
0.011541586369276047,
0.05712110549211502,
-0.04912574216723442,
-0.008456687442958355,
0.016061607748270035,
0.002189111430197954,
0.010892762802541256,
-0.026645157486200333,
-0.025750581175088882,
-0.016703983768820763,
0.00476651219651103,
-0.049434512853622437,
0.0411345511674881,
-0.013586515560746193,
-0.03582298383116722,
-0.03880753368139267,
0.3693046271800995,
0.05240253359079361,
0.020541012287139893,
-0.048529550433158875,
-0.016463572159409523,
0.030735410749912262,
-0.03555591031908989,
-0.007753307465463877,
0.01852824166417122,
-0.017922958359122276,
0.029286660254001617,
0.0437188558280468,
-0.007696896325796843,
0.043758902698755264,
-0.05943877622485161,
-0.08997965604066849,
0.025390971451997757,
0.08908344805240631,
0.05596562847495079,
-0.01564328558743,
0.039805810898542404,
-0.04421989992260933,
0.0077235219068825245,
0.03473667800426483,
-0.026111185550689697,
0.05972328782081604,
0.016168734058737755,
0.01738012582063675,
0.08718588203191757,
0.018307926133275032,
0.015341104008257389,
0.0013713616644963622,
0.06304939836263657,
-0.01947524957358837,
-0.008853132836520672,
-0.017576253041625023,
0.0009421212016604841,
0.08709750324487686,
-0.1094944104552269,
0.029524587094783783,
0.09129322320222855,
-0.038041096180677414,
-0.051324643194675446,
-0.10914825648069382,
-0.04737032949924469,
0.0052271620370447636,
0.10916288197040558,
-0.0017923495033755898,
-0.012323017232120037,
-0.021051188930869102,
-0.001610026927664876,
0.01711125299334526,
0.055507220327854156,
-0.03784329071640968,
-0.0017827616538852453,
0.006140394136309624,
-0.007750982418656349,
-0.02634900063276291,
0.0172642283141613,
0.025453917682170868,
0.015634916722774506,
-0.08678364008665085,
-0.07968669384717941,
-0.022228356450796127,
0.014082266017794609,
0.032372649759054184,
0.018439484760165215,
-0.01828450709581375,
-0.050914227962493896,
-0.02797195129096508,
0.04018742963671684,
-0.02535630203783512,
0.01911156252026558,
-0.012276136316359043,
-0.01727178879082203,
0.013381578959524632,
-0.027042057365179062,
-0.0008409353904426098,
-0.013023493811488152,
-0.027999697253108025,
-0.11760494112968445,
0.035928864032030106,
-0.009997539222240448,
-0.030820459127426147,
0.017749635502696037,
0.06471595913171768,
-0.030105985701084137,
-0.0322771817445755,
0.008929846808314323,
-0.004878268577158451,
-0.002282681642100215,
-0.02604549750685692,
0.04017038643360138,
-0.08795615285634995,
-0.04508592188358307,
0.019849682226777077,
-0.033109042793512344,
-0.027746694162487984,
0.00815243273973465,
-0.02991507388651371,
0.003997851628810167,
0.08165115118026733,
0.02724778652191162,
0.05694589391350746,
0.02582135796546936,
-0.004286800045520067,
0.018197981640696526,
-0.007642197422683239,
0.03613249957561493,
0.06054230406880379,
-0.01938062720000744,
0.007738071959465742,
0.04294486716389656,
0.0025145511608570814,
0.06713230162858963,
-0.03147958591580391,
0.05842059105634689,
-0.02544177882373333,
0.02428499236702919,
0.06256575137376785,
-0.030537137761712074,
0.050645291805267334,
-0.031599823385477066,
-0.04288138076663017,
-0.32978758215904236,
-0.0081632686778903,
0.014965769834816456,
-0.009740605019032955,
0.07530684769153595,
-0.10881980508565903,
-0.005426153540611267,
-0.04554412141442299,
-0.04169974476099014,
-0.01051284745335579,
0.012794975191354752,
0.01805669255554676,
-0.026494521647691727,
-0.011436883360147476,
-0.0038857669569551945,
0.02157512493431568,
0.06397496908903122,
-0.05138858035206795,
0.0796913355588913,
0.016699275001883507,
0.028602009639143944,
-0.011643855832517147,
0.035633634775877,
0.02314895950257778,
0.04716208949685097,
-0.001683038310147822,
0.07222960889339447,
-0.0372721366584301,
0.002402250189334154,
-0.07297515869140625,
0.0012765913270413876,
0.007690525613725185,
0.004743542056530714,
-0.07969660311937332,
-0.0024488375056535006,
-0.036873966455459595,
0.027228377759456635,
-0.012167923152446747,
-0.03657109662890434,
-0.05468194931745529,
-0.07210433483123779,
0.028308100998401642,
-0.049005087465047836,
-0.05292927473783493,
-0.05064599961042404,
0.07366907596588135,
-0.03153476119041443,
0.057921383529901505,
-0.008697103708982468,
-0.032901231199502945,
0.08297991752624512,
0.02610105834901333,
0.01781761646270752,
-0.0014111800119280815,
0.04972071945667267,
-0.022989999502897263,
-0.06851731240749359,
-0.029185108840465546,
-0.06510595977306366,
-0.03723627328872681,
-0.019998302683234215,
0.011762868613004684,
-0.00404968298971653,
-0.011106672696769238,
0.0003435321559663862,
0.006309840828180313,
0.01898227073252201,
0.054940685629844666,
0.07623469829559326,
0.07820666581392288,
-0.0004959020297974348,
0.05903124436736107,
-0.03278956934809685,
0.05827287957072258,
0.0544033944606781,
0.033691707998514175,
-0.04811827838420868,
-0.030706478282809258,
-0.07838410139083862,
0.0026122883427888155,
-0.019904155284166336,
-0.07477273792028427,
0.06169749051332474,
0.02433554269373417,
-0.004090124741196632,
0.05744705721735954,
-0.005165318958461285,
-0.0034510919358581305,
-0.029385844245553017,
0.03329811245203018,
0.010129847563803196,
-0.0021922015585005283,
-0.0494920015335083,
-0.011849680915474892,
0.03254000470042229,
0.0037670766469091177,
-0.25851595401763916,
0.014624557457864285,
0.02086072601377964,
-0.026723481714725494,
-0.03863518312573433,
0.029740964993834496,
-0.016233110800385475,
0.0036611566320061684,
-0.02104860357940197,
0.03846036642789841,
0.0162150040268898,
0.021356891840696335,
0.06153381988406181,
-0.000813426508102566,
0.0038886950351297855,
-0.012529643252491951,
0.03930962085723877,
-0.02134777046740055,
-0.009140867739915848,
-0.02448488399386406,
0.06882841140031815,
-0.04205967113375664,
0.09529806673526764,
-0.006719416938722134,
-0.01980854943394661,
0.05251610651612282,
0.008070741780102253,
0.0391993410885334,
0.0012464473256841302,
-0.015761245042085648,
-0.008401971310377121,
0.07469206303358078,
0.018885817378759384,
0.10124678164720535,
0.02581622824072838,
0.03571368008852005,
-0.045759279280900955,
0.05032319948077202,
-0.007306397892534733,
0.003719839034602046,
-0.009268348105251789,
-0.00038776081055402756,
-0.08970167487859726,
-0.013036387041211128,
0.040019772946834564,
-0.05574977025389671,
-0.03162674978375435,
-0.047482144087553024,
0.0182504840195179,
-0.02072256989777088,
-0.018953640013933182,
-0.06068021431565285,
0.07082926481962204,
0.08343788236379623,
0.01630420796573162,
-0.03095203824341297,
-0.02771252952516079,
-0.07133086025714874,
0.007503919769078493,
-0.03615754097700119,
0.0029451027512550354,
0.0037899324670434,
-0.06652068346738815,
0.039781659841537476,
-0.012201517820358276
] |
PMID:18682
|
The scientific foundations of clinical enzymology.
|
Clinical enzymology is the study of the activity and properties of enzymes in specimens (usually of blood) taken from patients, as an aid to the diagnosis and understanding of disease. Research in many areas of enzymology is undertaken to strengthen the foundations on which the clinical interpretation of such enzyme measurements rests. Some aspects of this research are briefly described.
|
The scientific foundations of clinical enzymology. Clinical enzymology is the study of the activity and properties of enzymes in specimens (usually of blood) taken from patients, as an aid to the diagnosis and understanding of disease. Research in many areas of enzymology is undertaken to strengthen the foundations on which the clinical interpretation of such enzyme measurements rests. Some aspects of this research are briefly described.
|
[
0.0029401429928839207,
0.06406821310520172,
-0.00190167676191777,
0.07454439252614975,
-0.013760593719780445,
-0.02459842525422573,
0.09572660177946091,
0.07737674564123154,
0.03882523626089096,
0.01639101281762123,
0.013496299274265766,
-0.05413207411766052,
-0.03114459104835987,
0.054801955819129944,
-0.04646638035774231,
-0.01359050627797842,
-0.0013204269343987107,
0.023016950115561485,
0.005199058447033167,
0.014980181120336056,
0.03826867416501045,
0.023250389844179153,
0.019063787534832954,
-0.057777103036642075,
0.027362871915102005,
0.041416775435209274,
-0.008984660729765892,
-0.0254062507301569,
-0.07338875532150269,
-0.07771646976470947,
-0.0016523926751688123,
-0.04891334846615791,
0.021296551451086998,
0.007627270650118589,
0.03323892503976822,
-0.013436539098620415,
0.01760798878967762,
0.01155981793999672,
-0.017924800515174866,
0.0528910867869854,
0.09902392327785492,
0.00849419366568327,
-0.01875736191868782,
0.06397554278373718,
-0.004959974437952042,
-0.004382635001093149,
-0.0618140883743763,
0.010076633654534817,
-0.040479857474565506,
-0.02235448732972145,
0.0013110340805724263,
-0.030038636177778244,
-0.01445978693664074,
0.018018396571278572,
-0.006632985081523657,
-0.02861788682639599,
0.03552641719579697,
-0.045698948204517365,
-0.015688996762037277,
0.04683130607008934,
0.03935704380273819,
-0.03466835990548134,
-0.16324065625667572,
0.015071318484842777,
0.07411573082208633,
-0.03540438786149025,
0.028792792931199074,
-0.0694989487528801,
0.05547434836626053,
0.017660625278949738,
-0.04503844678401947,
0.006722982041537762,
0.016928672790527344,
0.04990195110440254,
0.009935913607478142,
-0.008731651119887829,
-0.028758235275745392,
-0.013420438393950462,
0.029495680704712868,
0.06514765322208405,
0.005310377571731806,
-0.03535543382167816,
0.10856162011623383,
-0.0020154197700321674,
-0.01334531418979168,
0.0013937001349404454,
-0.019049661234021187,
-0.0736316666007042,
0.03541039675474167,
0.04975910857319832,
-0.0360894650220871,
-0.0678616389632225,
-0.0887749195098877,
0.06997042894363403,
-0.058707933872938156,
0.019499918445944786,
0.0725320503115654,
-0.013260314241051674,
0.029778461903333664,
0.3362433910369873,
-0.030123820528388023,
0.0094153368845582,
-0.03877861425280571,
-0.05025206133723259,
-0.012733775191009045,
-0.09484153240919113,
-0.05262668430805206,
0.021532971411943436,
-0.02556586265563965,
-0.014672699384391308,
-0.01884608529508114,
0.03348366543650627,
0.05841957777738571,
-0.06110135838389397,
-0.012663894332945347,
0.02916557528078556,
0.0008171513909474015,
0.05758960545063019,
-0.017503956332802773,
0.016221869736909866,
-0.006904458161443472,
-0.0137567650526762,
0.0038974827621132135,
-0.0027609567623585463,
0.042105332016944885,
0.002459065057337284,
0.03207416459918022,
0.09757503122091293,
0.06487761437892914,
-0.01833822950720787,
0.03812294080853462,
0.05333114042878151,
-0.07286220043897629,
0.0011274920543655753,
0.01853293552994728,
-0.02418227680027485,
0.06040789932012558,
0.00021131719404365867,
0.08562182635068893,
0.04500602185726166,
0.01564113423228264,
0.022975120693445206,
-0.07098112255334854,
-0.021658513695001602,
-0.1187969371676445,
0.059243205934762955,
-0.009755220264196396,
0.009068526327610016,
-0.02773246541619301,
-0.055618900805711746,
0.026775576174259186,
0.07848874479532242,
-0.02493172511458397,
0.03820911422371864,
-0.0392676517367363,
0.0201091468334198,
0.019410036504268646,
-0.07546309381723404,
0.013949702493846416,
-0.004924403503537178,
-0.09315016865730286,
-0.019872860983014107,
-0.011095781810581684,
0.08746906369924545,
0.04646315053105354,
0.008291784673929214,
0.010118851438164711,
0.011807996779680252,
0.010461824014782906,
-0.026698866859078407,
0.036056455224752426,
-0.010470320470631123,
-0.02762584201991558,
0.048111509531736374,
-0.059059809893369675,
0.011212100274860859,
-0.007688296027481556,
0.09628689289093018,
-0.05965784937143326,
0.014069018885493279,
0.07847493886947632,
0.023866020143032074,
-0.042068999260663986,
0.023384971544146538,
0.027134327217936516,
0.017350314185023308,
-0.014534483663737774,
0.018160363659262657,
0.03947486728429794,
0.0005240691243670881,
-0.01648508943617344,
-0.027431590482592583,
-0.1187877207994461,
-0.057806264609098434,
-0.002033513505011797,
-0.009189650416374207,
0.012969798408448696,
0.01727469451725483,
-0.04554850608110428,
0.02342514507472515,
0.004754514433443546,
0.05794788524508476,
0.0016243825666606426,
0.051470283418893814,
0.004329463467001915,
0.06987645477056503,
0.01118482742458582,
-0.020277248695492744,
0.011111077852547169,
0.009803665801882744,
-0.06086120381951332,
0.02657715231180191,
-0.07086969166994095,
-0.05515701696276665,
-0.06163780391216278,
0.012844660319387913,
0.006445561069995165,
-0.02493518963456154,
0.030224541202187538,
-0.01963266357779503,
-0.03961547464132309,
-0.03839130327105522,
-0.0019530694698914886,
-0.29009974002838135,
-0.0038277716375887394,
-0.021103067323565483,
0.0035250335931777954,
0.05290195345878601,
0.03862779960036278,
0.015062280930578709,
-0.040429554879665375,
-0.010994662530720234,
-0.028109516948461533,
0.04467446357011795,
0.018515126779675484,
-0.03194937855005264,
-0.06061706319451332,
-0.02225290983915329,
-0.04605137184262276,
0.03910348191857338,
-0.06149924546480179,
-0.021469298750162125,
-0.025443553924560547,
0.06143627688288689,
-0.014590336941182613,
0.1051323339343071,
-0.03721596673130989,
0.022123068571090698,
-0.004916110076010227,
0.06756040453910828,
-0.0304869394749403,
0.032636336982250214,
0.008403348736464977,
-0.01878531463444233,
0.02430417761206627,
-0.07246976345777512,
-0.14219945669174194,
-0.06479337811470032,
-0.092524453997612,
-0.017878608778119087,
-0.009901808574795723,
-0.043906450271606445,
-0.01249559037387371,
-0.010458428412675858,
0.002233411418274045,
-0.012252701446413994,
0.029712023213505745,
-0.015988314524292946,
0.062150828540325165,
0.07226093113422394,
0.0036494871601462364,
-0.0035431876312941313,
-0.007813207805156708,
-0.029590535908937454,
-0.047978319227695465,
-0.02215082198381424,
-0.029145818203687668,
-0.02636372111737728,
0.02915431372821331,
-0.06879730522632599,
0.00971167627722025,
0.00867179874330759,
0.013843857683241367,
-0.015880512073636055,
0.04829259216785431,
0.01567487046122551,
-0.010995675809681416,
0.006922074593603611,
-0.031328119337558746,
0.012585962191224098,
-0.03315787389874458,
0.05618087202310562,
-0.011537711136043072,
0.004955347161740065,
0.09850646555423737,
-0.07322371006011963,
-0.0036674931179732084,
0.009558066725730896,
0.029527731239795685,
-0.0024093622341752052,
-0.035941559821367264,
-0.06049157306551933,
-0.006975016091018915,
-0.0015796232037246227,
0.0023333497811108828,
0.023062540218234062,
-0.0439877063035965,
0.06386478990316391,
0.018140314146876335,
0.07757773250341415,
-0.05497162416577339,
0.0031496533192694187,
-0.03282110020518303,
-0.016275275498628616,
0.012227821163833141,
-0.04106694459915161,
-0.04998704418540001,
-0.02715780958533287,
0.036145955324172974,
-0.2550821602344513,
0.03351712226867676,
-0.0028327410109341145,
0.010846215300261974,
0.017345966771245003,
0.03318479657173157,
0.005664211697876453,
0.06098749488592148,
-0.02070804499089718,
-0.010631246492266655,
0.04316681995987892,
-0.05950761213898659,
0.05734579637646675,
-0.007418249733746052,
-0.028523223474621773,
0.001947763841599226,
0.1051812618970871,
-0.032850246876478195,
0.0019645458087325096,
-0.06413324922323227,
0.06388093531131744,
-0.01912073977291584,
0.1086219847202301,
-0.03007933869957924,
0.060380663722753525,
0.08581843972206116,
0.011689135804772377,
0.047980114817619324,
-0.06760486215353012,
-0.01654723472893238,
0.06443745642900467,
-0.01726587861776352,
0.05850524082779884,
-0.0338466577231884,
0.04127195104956627,
0.05267472192645073,
0.007300771307200193,
0.06003087759017944,
0.03569153696298599,
-0.00519990548491478,
0.026114342734217644,
-0.018204258754849434,
-0.08280033618211746,
-0.013466202653944492,
0.047439463436603546,
0.0011664986377581954,
0.017629608511924744,
-0.04311779513955116,
-0.024492112919688225,
0.008390447124838829,
-0.010474041104316711,
-0.05425218492746353,
-0.01854320988059044,
0.022133342921733856,
0.04346548765897751,
-0.011558066122233868,
-0.009147035889327526,
-0.0349033921957016,
0.02917066030204296,
-0.05036946386098862,
-0.007725904695689678,
0.058612801134586334,
-0.024886123836040497,
0.16532264649868011,
-0.06115053966641426
] |
PMID:18683
|
Modification of actins by phallotoxins.
|
Phallotoxins bind to filamentous actin (F-actin) from liver or rabbit muscle with Kdiss approximately 10(-8) M. By this combination the structure of F-actin is stabilized to such an extent that it will be resistant to the depolymerizing action of 0.6 M KI and of deoxyribonuclease I, to denaturation by heat (70 degrees C), and to local ruptures caused by ultrasonication or by cytochalasin B. The structural features of phallotoxins essential for affinity to F-actin are specific.
|
Modification of actins by phallotoxins. Phallotoxins bind to filamentous actin (F-actin) from liver or rabbit muscle with Kdiss approximately 10(-8) M. By this combination the structure of F-actin is stabilized to such an extent that it will be resistant to the depolymerizing action of 0.6 M KI and of deoxyribonuclease I, to denaturation by heat (70 degrees C), and to local ruptures caused by ultrasonication or by cytochalasin B. The structural features of phallotoxins essential for affinity to F-actin are specific.
|
[
-0.0909244492650032,
-0.05955136939883232,
0.04995623230934143,
0.004501114133745432,
0.0393628254532814,
0.009933801367878914,
0.10292751342058182,
0.040291670709848404,
-0.04552837088704109,
0.014158401638269424,
0.0009180788183584809,
-0.04773976281285286,
-0.03237316757440567,
0.051008887588977814,
-0.015491077676415443,
-0.036217156797647476,
-0.014580462127923965,
0.04153984412550926,
-0.05777331814169884,
-0.010092661716043949,
0.011278749443590641,
0.012903468683362007,
0.013308659195899963,
0.038826148957014084,
0.056083422154188156,
0.020452681928873062,
0.008331742137670517,
0.01104993000626564,
-0.024504628032445908,
-0.2017078995704651,
0.031841110438108444,
-0.026063544675707817,
0.008459574542939663,
-0.026954859495162964,
-0.03838367387652397,
0.016120972111821175,
0.02194616198539734,
-0.049065686762332916,
0.027529306709766388,
0.034933775663375854,
0.07654246687889099,
0.08027182519435883,
0.01233841385692358,
-0.02201659418642521,
0.05650978535413742,
0.04279325157403946,
-0.0435829721391201,
0.0129984300583601,
0.06712482124567032,
0.03445425257086754,
0.05078565701842308,
-0.08825352042913437,
0.016993798315525055,
0.09122117608785629,
0.047383036464452744,
-0.026116682216525078,
0.03905300050973892,
0.0688285380601883,
0.02265198715031147,
-0.019159816205501556,
0.001382407615892589,
0.01533616054803133,
-0.09190623462200165,
0.06677460670471191,
0.07279961556196213,
0.04016881063580513,
0.05863470211625099,
0.010455996729433537,
0.022582199424505234,
0.05309349671006203,
-0.058409325778484344,
0.034935932606458664,
-0.040156617760658264,
0.03241003304719925,
0.017947489395737648,
-0.025904670357704163,
-0.03661675006151199,
-0.02201523818075657,
0.02758420631289482,
0.02161410264670849,
-0.057465068995952606,
0.009750692173838615,
0.031421106308698654,
-0.03620713949203491,
-0.02892392873764038,
0.036079902201890945,
0.0363239049911499,
-0.03901136666536331,
-0.02896241471171379,
0.03996985778212547,
-0.0356881208717823,
-0.06672849506139755,
0.03768123313784599,
-0.02278963476419449,
-0.055923279374837875,
0.08068552613258362,
-0.008530888706445694,
-0.026377340778708458,
-0.008133665658533573,
0.3572612404823303,
-0.06724236160516739,
0.014929776079952717,
-0.0704365223646164,
0.02437749132514,
0.00575705012306571,
0.0019697751849889755,
0.010577768087387085,
-0.026167020201683044,
-0.03863617032766342,
0.047990962862968445,
0.00006720470264554024,
0.024598047137260437,
-0.049109939485788345,
-0.008580300956964493,
0.01702011562883854,
0.014582289382815361,
-0.022859131917357445,
0.034612324088811874,
-0.02789551019668579,
0.03897618129849434,
-0.02310522273182869,
0.05227910727262497,
0.030876342207193375,
-0.035589028149843216,
0.025553828105330467,
0.01183452270925045,
-0.010047114454209805,
0.058631040155887604,
0.07501453161239624,
0.03534690663218498,
-0.006552023347467184,
0.01888846419751644,
-0.02154376730322838,
-0.05463826283812523,
-0.03741271048784256,
0.02533773146569729,
0.017196450382471085,
-0.059176113456487656,
-0.009167290292680264,
0.008780354633927345,
-0.018901193514466286,
-0.09674808382987976,
-0.025545844808220863,
-0.06747190654277802,
-0.018764495849609375,
0.06117044389247894,
-0.030641965568065643,
-0.0008305396186187863,
-0.022185249254107475,
0.04334835708141327,
-0.007050264161080122,
0.032132990658283234,
-0.018818700686097145,
0.01102436799556017,
0.02453441545367241,
-0.004163364414125681,
-0.03264382854104042,
-0.022455213591456413,
0.01770327240228653,
-0.06104175001382828,
-0.127054825425148,
-0.004658403340727091,
-0.011531142517924309,
0.05790766328573227,
0.0442720390856266,
-0.00736681604757905,
-0.012162098661065102,
-0.0005291355773806572,
0.019817670807242393,
0.00014976007514633238,
0.017393581569194794,
-0.010169195011258125,
-0.0449274517595768,
-0.02677628956735134,
-0.059312015771865845,
0.04030048847198486,
-0.0038701859302818775,
-0.06073320284485817,
0.04977178946137428,
0.02234882116317749,
0.02340194769203663,
-0.041254885494709015,
-0.0028818687424063683,
-0.004797216039150953,
0.03219718858599663,
-0.05548730120062828,
-0.05430246517062187,
0.07211512327194214,
0.03141559287905693,
0.06733214110136032,
-0.028160931542515755,
0.04498600959777832,
-0.06673700362443924,
0.053560514003038406,
-0.07119811326265335,
-0.07661585509777069,
-0.05153790861368179,
0.04853285104036331,
-0.03295474126935005,
0.019775524735450745,
0.13409626483917236,
0.0239925105124712,
-0.03302253782749176,
0.06805602461099625,
-0.012211023829877377,
0.010622059926390648,
0.0200286116451025,
0.007154365535825491,
0.011777717620134354,
-0.026445340365171432,
-0.10332998633384705,
0.018992943689227104,
-0.00410730903968215,
-0.06612653285264969,
-0.025131765753030777,
-0.027583912014961243,
-0.0015618352917954326,
-0.015816781669855118,
-0.007112463936209679,
0.008010915480554104,
0.07155701518058777,
-0.049217868596315384,
0.01651044376194477,
-0.3093414604663849,
0.004395294468849897,
-0.027797555550932884,
-0.02486409619450569,
0.07807890325784683,
-0.01867098920047283,
0.04088418930768967,
-0.029234789311885834,
0.026207618415355682,
-0.015530668199062347,
0.02951025404036045,
0.058208826929330826,
-0.043576810508966446,
-0.0738331750035286,
-0.008573990315198898,
0.004550390876829624,
0.04317658394575119,
-0.045812882483005524,
0.04490210488438606,
0.04249207675457001,
-0.016609419137239456,
0.01878180168569088,
-0.027507634833455086,
0.05692658573389053,
0.06514589488506317,
0.06277985125780106,
0.1517815887928009,
0.022381668910384178,
-0.01942024752497673,
-0.0064697349444031715,
0.025065110996365547,
0.005977521184831858,
-0.08687923848628998,
-0.02407698892056942,
-0.02319708652794361,
0.022260185331106186,
0.04247337579727173,
-0.051119621843099594,
0.052224498242139816,
0.03260170295834541,
-0.04713666811585426,
0.08409038186073303,
-0.003210339928045869,
-0.09536703675985336,
-0.03272944688796997,
-0.020861489698290825,
0.0006088793743401766,
0.017887448891997337,
-0.006055355072021484,
0.013981246389448643,
0.043052591383457184,
-0.02195526473224163,
-0.024198094382882118,
0.02883814089000225,
-0.07605446875095367,
-0.004141444806009531,
0.009621077217161655,
0.009696012362837791,
-0.027652908116579056,
0.024939240887761116,
-0.036591555923223495,
-0.016599930822849274,
0.011419767513871193,
-0.013829667121171951,
-0.002512955805286765,
0.028236331418156624,
0.030502254143357277,
0.0037811570800840855,
-0.0016221945406869054,
0.02549644559621811,
-0.016400856897234917,
0.06983048468828201,
0.016360267996788025,
-0.002583019668236375,
0.010672448202967644,
-0.00178548286203295,
-0.05490567907691002,
-0.0013035341398790479,
-0.008214045315980911,
0.038223396986722946,
0.01939743384718895,
-0.016567613929510117,
0.06051848456263542,
-0.04931424930691719,
-0.019418327137827873,
-0.010588780976831913,
-0.011308610439300537,
-0.023671802133321762,
0.01264331303536892,
-0.023233044892549515,
-0.002751043764874339,
0.012754276394844055,
-0.05576278641819954,
-0.04752691462635994,
0.04651347175240517,
-0.01731836423277855,
-0.23298463225364685,
-0.009113096632063389,
0.08332628011703491,
0.02110334299504757,
-0.05538306385278702,
0.05013241246342659,
0.054830051958560944,
-0.021987002342939377,
0.007826380431652069,
0.06624928116798401,
-0.04179917275905609,
0.03474701568484306,
0.06504979729652405,
-0.0399642288684845,
-0.013924828730523586,
-0.011834101751446724,
0.07059706747531891,
-0.015183397568762302,
0.005476599093526602,
-0.02973439358174801,
-0.0227127056568861,
-0.024735480546951294,
0.14757724106311798,
-0.05606372281908989,
-0.0038070804439485073,
0.0024732942692935467,
0.005157367326319218,
0.028667423874139786,
-0.026879219338297844,
-0.016791021451354027,
-0.0021491064690053463,
0.020523613318800926,
0.028168614953756332,
-0.02304948680102825,
-0.02092759683728218,
0.03180839493870735,
0.012530749663710594,
-0.04673216864466667,
-0.024644434452056885,
0.03233547508716583,
-0.008256684057414532,
-0.04736696928739548,
-0.07867481559515,
-0.07111838459968567,
0.041272398084402084,
-0.0013148599537089467,
-0.07729966193437576,
-0.07606232166290283,
-0.03887190669775009,
0.017406698316335678,
-0.07175415009260178,
0.001261725090444088,
0.04609883576631546,
0.033515360206365585,
0.0025270062033087015,
-0.08782751858234406,
0.013832214288413525,
-0.005252002738416195,
0.0061913724057376385,
0.024171629920601845,
0.02383239194750786,
0.08564569056034088,
-0.061633799225091934,
0.07949087023735046,
0.020383257418870926
] |
PMID:18684
|
Characteristics of chick cerebral beta-adrenoceptors assessed by cyclic adenosine 3',5' monophosphate formation and [3H]-propranolol binding.
|
Chick cerebral beta-adrenoceptors have been characterised by measurement of cyclic AMP accumulation in brain slices and assessment of the specific binding of [3H]-propranolol to cerebral membranes. The binding of [3H]-propranolol was inhibited by beta-adrenoceptor agonists and antagonists with affinities that correlated well with their ability to stimulate cyclic AMP formation or to antagonise the cyclic nucleotide accumulation induced by isoprenaline. The relative potencies of a number of drugs in several cerebral regions suggests that the receptors may be of the beta2 subtype. Regional distribution studies revealed the highest density of binding sites in the cerebellum and lowest in the optic lobes. However, the concentration of [3H]-propranolol that produced half-maximal specific binding was similar in all regions. Subcellular fractionation of cerebral hemisphere tissue demonstrated an enrichment of [3H]-propranolol binding sites in the synaptosomal and microsomal fractions. There are discrepancies between the topographical distribution of beta-adrenoceptor binding sites and the endogenous noradrenaline level in chick brain and between the number of binding sites and the intrinsic activity of beta-adrenoceptor mediated cyclic AMP formation.
|
Characteristics of chick cerebral beta-adrenoceptors assessed by cyclic adenosine 3',5' monophosphate formation and [3H]-propranolol binding. Chick cerebral beta-adrenoceptors have been characterised by measurement of cyclic AMP accumulation in brain slices and assessment of the specific binding of [3H]-propranolol to cerebral membranes. The binding of [3H]-propranolol was inhibited by beta-adrenoceptor agonists and antagonists with affinities that correlated well with their ability to stimulate cyclic AMP formation or to antagonise the cyclic nucleotide accumulation induced by isoprenaline. The relative potencies of a number of drugs in several cerebral regions suggests that the receptors may be of the beta2 subtype. Regional distribution studies revealed the highest density of binding sites in the cerebellum and lowest in the optic lobes. However, the concentration of [3H]-propranolol that produced half-maximal specific binding was similar in all regions. Subcellular fractionation of cerebral hemisphere tissue demonstrated an enrichment of [3H]-propranolol binding sites in the synaptosomal and microsomal fractions. There are discrepancies between the topographical distribution of beta-adrenoceptor binding sites and the endogenous noradrenaline level in chick brain and between the number of binding sites and the intrinsic activity of beta-adrenoceptor mediated cyclic AMP formation.
|
[
-0.011774353682994843,
-0.05686682090163231,
-0.04669162631034851,
-0.0027493201196193695,
0.016370128840208054,
0.07049454748630524,
0.045485515147447586,
0.027726998552680016,
-0.02256852388381958,
-0.012704889290034771,
0.03239022195339203,
-0.07312540709972382,
-0.015483006834983826,
0.05388787388801575,
-0.05762031674385071,
0.0014779729535803199,
0.001138911466114223,
-0.05103129893541336,
-0.01282029040157795,
-0.03953215479850769,
0.014047353528439999,
-0.03806015104055405,
-0.006072863005101681,
0.020252687856554985,
0.01671767048537731,
0.003933179657906294,
-0.021997319534420967,
-0.0052719865925610065,
-0.09337852150201797,
-0.2045396864414215,
-0.008875942789018154,
0.009154440835118294,
0.034317586570978165,
-0.0546102412045002,
-0.036114659160375595,
-0.014762487262487411,
0.0043327403254806995,
0.024069560691714287,
-0.02413499914109707,
0.05226216837763786,
0.04043664410710335,
-0.03640880808234215,
-0.009771790355443954,
0.00006709441368002445,
-0.015380791388452053,
-0.039057403802871704,
-0.07723547518253326,
-0.04438570886850357,
0.11572152376174927,
0.002127894666045904,
0.002546157920733094,
-0.04559924453496933,
-0.027304748073220253,
0.03597000986337662,
-0.018343402072787285,
-0.0006783773424103856,
-0.01975577510893345,
0.02140316739678383,
0.016237104311585426,
0.033995408564805984,
-0.05360059067606926,
0.029781276360154152,
-0.144463449716568,
0.051004182547330856,
0.03373205289244652,
0.037555478513240814,
-0.019293509423732758,
-0.02920113317668438,
0.04014921560883522,
0.03960470110177994,
-0.01797161065042019,
0.040851421654224396,
0.009914176538586617,
-0.03543410822749138,
0.004138406366109848,
0.00795511994510889,
-0.027037877589464188,
0.005543431732803583,
0.033666037023067474,
-0.032947372645139694,
0.021441051736474037,
0.02878592163324356,
-0.0055824932642281055,
-0.030015159398317337,
-0.00220872787758708,
-0.014276610687375069,
0.04466477409005165,
-0.0284611526876688,
-0.013955473899841309,
-0.020097943022847176,
-0.06266698241233826,
-0.05619752034544945,
-0.024374758824706078,
-0.019529597833752632,
-0.03737238794565201,
0.021115703508257866,
-0.028569083660840988,
-0.0434233620762825,
-0.031304046511650085,
0.33514294028282166,
0.0068509276024997234,
0.05744095519185066,
-0.05165750905871391,
0.023147977888584137,
0.021449020132422447,
-0.012582172639667988,
-0.0020908250007778406,
-0.044243283569812775,
-0.03576255217194557,
0.04841716215014458,
-0.0028687312733381987,
-0.012959201820194721,
0.021128445863723755,
-0.055490631610155106,
-0.022437619045376778,
-0.08211692422628403,
-0.008495238609611988,
-0.0387447290122509,
-0.009424153715372086,
-0.006634592544287443,
-0.046839389950037,
-0.028980422765016556,
0.013261069543659687,
0.038955677300691605,
0.06703359633684158,
0.0030439894180744886,
-0.04597284644842148,
0.09386176615953445,
0.08420203626155853,
-0.0027057223487645388,
0.08738245815038681,
0.0790257453918457,
-0.04260062426328659,
0.04795727878808975,
0.03677741065621376,
-0.009405259974300861,
0.0488828606903553,
-0.05813015624880791,
-0.008548371493816376,
-0.016714781522750854,
-0.006772231310606003,
-0.045010924339294434,
0.05705928057432175,
-0.014887788332998753,
-0.035738278180360794,
0.1291963905096054,
0.017302222549915314,
-0.0151809211820364,
-0.06745495647192001,
0.0648806095123291,
-0.07534673810005188,
0.08266466856002808,
0.01581149362027645,
0.07735218852758408,
-0.03592967987060547,
0.0546032078564167,
0.002846378367394209,
0.010907323099672794,
-0.036083023995161057,
-0.02369341440498829,
-0.07806026935577393,
-0.033631544560194016,
0.025542359799146652,
0.06293066591024399,
0.0017950910842046142,
0.0024120910093188286,
-0.045159775763750076,
-0.017687486484646797,
0.012145299464464188,
0.03642960637807846,
0.0043289135210216045,
0.014830327592790127,
0.015067953616380692,
-0.04289643093943596,
-0.04164467379450798,
0.06924252212047577,
0.004574821330606937,
0.027445631101727486,
0.058967120945453644,
-0.07794509828090668,
-0.008216047659516335,
0.006241126451641321,
-0.017135310918092728,
0.008264577947556973,
0.01469178032130003,
-0.0062944614328444,
-0.010582910850644112,
-0.024171579629182816,
-0.03800901025533676,
0.04452357068657875,
-0.04708601534366608,
-0.013848456554114819,
-0.07236155867576599,
-0.011489630676805973,
-0.060076866298913956,
-0.02332630380988121,
0.005424226634204388,
0.02552146464586258,
-0.017069334164261818,
0.027770541608333588,
0.07010208070278168,
-0.01306131761521101,
-0.0399630144238472,
0.0763758197426796,
0.024206917732954025,
0.053898297250270844,
0.006672144867479801,
-0.002402699086815119,
0.07277335226535797,
0.043430618941783905,
-0.006983631756156683,
0.027284443378448486,
0.05506107211112976,
-0.043558813631534576,
-0.0539240725338459,
0.034168507903814316,
-0.03326554223895073,
0.00950497854501009,
0.0481550507247448,
0.02460896223783493,
0.029484843835234642,
-0.044692154973745346,
0.005079352762550116,
-0.2961604595184326,
-0.056271784007549286,
0.005600443575531244,
-0.015877431258559227,
0.04527967795729637,
-0.029868220910429955,
0.06372875720262527,
0.0051016733050346375,
0.008383190259337425,
-0.05898323282599449,
0.006237114313989878,
0.021884731948375702,
-0.01567820832133293,
-0.018520476296544075,
-0.05012262985110283,
0.06983527541160583,
0.0162033811211586,
-0.04515025019645691,
0.020632900297641754,
0.010294527746737003,
-0.0006503143813461065,
0.029733117669820786,
-0.05949096009135246,
0.04377509653568268,
0.033716026693582535,
-0.022907808423042297,
0.07460418343544006,
-0.01830858364701271,
-0.0016428801463916898,
0.011251784861087799,
0.032775864005088806,
0.016346493735909462,
0.03525792062282562,
-0.09741275012493134,
-0.004319765605032444,
-0.0231339018791914,
-0.01630544289946556,
-0.015517170540988445,
0.027835199609398842,
0.02032976970076561,
-0.06352269649505615,
0.0497734397649765,
0.009218691848218441,
-0.0030057684052735567,
-0.05985978618264198,
-0.0020592876244336367,
-0.008460287936031818,
0.06577993929386139,
0.011304214596748352,
-0.006703355815261602,
0.024848831817507744,
0.04311779886484146,
-0.027854444459080696,
-0.07866019755601883,
0.05356472358107567,
0.04169662669301033,
-0.059174515306949615,
0.0025516273453831673,
-0.04636505991220474,
0.021820159628987312,
0.0412483848631382,
-0.02503538876771927,
-0.016657378524541855,
0.01734280213713646,
0.0025121665094047785,
0.05602698028087616,
0.04576703906059265,
-0.0266290083527565,
0.030430227518081665,
0.048451751470565796,
0.0810093954205513,
0.06064832955598831,
-0.029964523389935493,
0.04554005339741707,
0.08431511372327805,
-0.022667083889245987,
-0.003716766368597746,
-0.05120337754487991,
-0.021389881148934364,
0.033770471811294556,
0.016997823491692543,
-0.047585099935531616,
0.03172896429896355,
0.02927088364958763,
-0.04149005934596062,
-0.0026858504861593246,
0.026495879516005516,
0.00514984643086791,
0.10100555419921875,
-0.036264169961214066,
-0.019601039588451385,
0.017476903274655342,
-0.04732109233736992,
-0.08843725174665451,
0.07373801618814468,
0.06685074418783188,
-0.27982500195503235,
0.003524935571476817,
0.043832771480083466,
0.025295455008745193,
0.04943624511361122,
0.016711227595806122,
0.03409772738814354,
-0.03480149805545807,
-0.03265341743826866,
0.01795188896358013,
-0.008612150326371193,
0.06418295949697495,
0.07731811702251434,
0.05872778594493866,
-0.0693904235959053,
0.018539035692811012,
0.034708645194768906,
-0.048144713044166565,
0.020686032250523567,
-0.06020523980259895,
0.0004875565064139664,
-0.0016624366398900747,
0.16027329862117767,
0.010853391140699387,
0.001374683459289372,
0.0093452837318182,
0.020288771018385887,
0.008286288008093834,
-0.07548347860574722,
0.01963239163160324,
-0.00213179225102067,
-0.005359061062335968,
0.0637814998626709,
-0.06463699787855148,
0.04681481793522835,
0.07259388267993927,
-0.052623603492975235,
-0.020276159048080444,
0.03849922865629196,
-0.017047788947820663,
-0.00019892129057552665,
0.06613658368587494,
-0.08618307113647461,
0.014864661730825901,
0.08555328845977783,
-0.06662677973508835,
-0.029227858409285545,
-0.021348731592297554,
0.032194655388593674,
-0.010137642733752728,
-0.04966355115175247,
-0.03733751177787781,
0.0006099407910369337,
0.017948752269148827,
-0.019296392798423767,
-0.02818886563181877,
-0.000502309063449502,
0.003011879976838827,
-0.007798666134476662,
0.010715571232140064,
-0.054854776710271835,
-0.026038672775030136,
0.004806326236575842,
0.09401489794254303,
0.08269853889942169
] |
PMID:18686
|
On the optimal dosage of Pro-Leu-Gly-NH2 (MIF) in neuropharmacological tests and clinical use.
|
Pro-Leu-Gly-NH2 (MIF) inhibits the tremor induced by oxotremorine. Objective measurement of this tremor permits the drawing of a dose-effect curve. The inhibitory effect of the peptide increases linearly with increasing doses until an optimum is reached (between 30 and 40 mg/kg i.p.). At still higher doses the peptide is inactive. The same phenomenon is observed with analogues of MIF. This finding may have important bearings on the interpretation of clinical and experimental data obtained with MIF.
|
On the optimal dosage of Pro-Leu-Gly-NH2 (MIF) in neuropharmacological tests and clinical use. Pro-Leu-Gly-NH2 (MIF) inhibits the tremor induced by oxotremorine. Objective measurement of this tremor permits the drawing of a dose-effect curve. The inhibitory effect of the peptide increases linearly with increasing doses until an optimum is reached (between 30 and 40 mg/kg i.p.). At still higher doses the peptide is inactive. The same phenomenon is observed with analogues of MIF. This finding may have important bearings on the interpretation of clinical and experimental data obtained with MIF.
|
[
-0.09643564373254776,
-0.007715209852904081,
-0.024308236315846443,
-0.016995001584291458,
-0.00770805636420846,
0.0359666533768177,
0.039882466197013855,
0.04204876348376274,
-0.024408608675003052,
-0.01008322462439537,
0.04531437158584595,
-0.0357346385717392,
0.03876553848385811,
0.05603500455617905,
-0.05871022492647171,
-0.007475277408957481,
0.006479540374130011,
-0.014089079573750496,
-0.023503046482801437,
-0.003234914503991604,
0.01752973534166813,
-0.02152951993048191,
0.01812303438782692,
-0.015388230793178082,
-0.020020071417093277,
0.05096173658967018,
-0.04646310955286026,
0.019239462912082672,
-0.004881124012172222,
-0.20388200879096985,
0.06285995244979858,
0.035904452204704285,
0.0010613249614834785,
-0.045958925038576126,
-0.07491584122180939,
0.025411218404769897,
-0.040656279772520065,
0.010272623971104622,
0.03858574852347374,
-0.007969730533659458,
0.03669273480772972,
-0.007265589199960232,
0.007318485528230667,
-0.0012381852138787508,
0.03335556015372276,
0.020564135164022446,
-0.054222483187913895,
-0.0038792917039245367,
0.07309585064649582,
0.08885551244020462,
-0.02571128122508526,
-0.0013509560376405716,
0.08146195113658905,
0.011625664308667183,
0.01949487254023552,
0.0015181455528363585,
-0.008781122975051403,
-0.00868378859013319,
-0.02186480537056923,
0.016053376719355583,
-0.0315445251762867,
0.031350672245025635,
-0.17327524721622467,
0.042258091270923615,
0.015189663507044315,
0.007548270281404257,
0.0010980182560160756,
-0.05150074511766434,
0.043290138244628906,
0.08537590503692627,
-0.03804977238178253,
0.04325610026717186,
-0.015478593297302723,
-0.020095180720090866,
0.048876840621232986,
0.018147800117731094,
0.0001125773778767325,
-0.00924128107726574,
0.03407737985253334,
0.004965648986399174,
0.02690906822681427,
0.012496747076511383,
-0.002782099414616823,
-0.03453228250145912,
0.04067206010222435,
-0.0024512859527021646,
0.015457133762538433,
0.007575212046504021,
-0.04033380374312401,
0.006954561453312635,
-0.033807381987571716,
0.0019986091647297144,
0.02416430599987507,
-0.03440200909972191,
-0.012556911446154118,
0.004721398930996656,
0.045622117817401886,
0.06852370500564575,
-0.023130079731345177,
0.38221853971481323,
-0.01956448145210743,
-0.004244101699441671,
-0.09033100306987762,
-0.011504221707582474,
0.031873591244220734,
-0.03024361841380596,
0.001461038482375443,
0.013316292315721512,
0.01994243450462818,
0.0011992979561910033,
0.06061773747205734,
0.022427981719374657,
0.07332484424114227,
-0.03996589779853821,
0.01522654015570879,
0.047319475561380386,
0.036151591688394547,
-0.005714185070246458,
-0.028849530965089798,
0.010017612017691135,
0.0122003685683012,
-0.039266470819711685,
0.06724830716848373,
-0.008351744152605534,
-0.015247060917317867,
-0.03942330554127693,
-0.05034884437918663,
0.04245278611779213,
0.036944203078746796,
-0.013457084074616432,
-0.008112098090350628,
-0.020902447402477264,
0.0029556001536548138,
0.0025078237522393465,
0.020614266395568848,
0.010013441555202007,
0.0005482794367708266,
-0.022847944870591164,
0.0038508360739797354,
0.07690983265638351,
0.019762329757213593,
-0.009108271449804306,
-0.05019625648856163,
-0.0976654663681984,
-0.048560064285993576,
0.06394995003938675,
0.011441393755376339,
-0.017358887940645218,
-0.006243679206818342,
0.028259500861167908,
-0.018185757100582123,
0.032123222947120667,
-0.04365919530391693,
0.01722634583711624,
-0.017853274941444397,
0.037612393498420715,
-0.005878970958292484,
-0.0233780425041914,
-0.02252793498337269,
-0.0203268900513649,
-0.0887574777007103,
-0.027784178033471107,
-0.027213646098971367,
0.06881280988454819,
-0.05360386148095131,
-0.013131337240338326,
-0.04684717580676079,
0.0006591469864360988,
-0.03631497919559479,
-0.018307672813534737,
0.017089547589421272,
0.0013807122595608234,
-0.025937344878911972,
-0.0033244118094444275,
0.015524527989327908,
-0.05361666530370712,
0.0013394984416663647,
0.002926196437329054,
0.015915323048830032,
-0.0024499380961060524,
0.009140107780694962,
-0.02164875715970993,
-0.020835356786847115,
-0.03831658884882927,
0.011904832907021046,
-0.014440570026636124,
-0.013581653125584126,
0.03519926220178604,
0.050553109496831894,
-0.0019241017289459705,
-0.030713727697730064,
0.006913239136338234,
-0.06147953122854233,
0.02933378331363201,
-0.010768461041152477,
-0.07108122110366821,
-0.10707996040582657,
-0.0015745098935440183,
-0.0355546772480011,
-0.0014782892540097237,
0.1028931736946106,
0.018858427181839943,
0.051474638283252716,
0.00031781874713487923,
0.04380572959780693,
0.016603145748376846,
-0.011866394430398941,
0.03166923299431801,
0.014979125000536442,
-0.03118564374744892,
-0.010938781313598156,
-0.03409455716609955,
0.023492619395256042,
0.0129234092310071,
-0.04440036416053772,
0.0439128652215004,
-0.0189953800290823,
0.03319326415657997,
0.02737843617796898,
0.000222488641156815,
0.057108137756586075,
-0.04651927947998047,
-0.10467582941055298,
-0.31849876046180725,
-0.03737788274884224,
0.02516818419098854,
-0.02432364970445633,
0.0820360854268074,
-0.0743362084031105,
0.00959249772131443,
-0.045332979410886765,
0.015469695441424847,
-0.0279823150485754,
0.05130152776837349,
0.04634808748960495,
-0.09674155712127686,
-0.027203386649489403,
0.01735112816095352,
0.023046568036079407,
0.06322029232978821,
-0.01715087704360485,
-0.012173887342214584,
0.019092002883553505,
-0.0019121598452329636,
0.03524310514330864,
-0.051629990339279175,
0.014319400303065777,
0.016834145411849022,
0.013370335102081299,
0.12255115061998367,
-0.03398776426911354,
-0.0394401028752327,
-0.08163633197546005,
0.07031533867120743,
0.052011530846357346,
0.008632957004010677,
-0.08038583397865295,
-0.02903270721435547,
-0.02275088243186474,
0.04166254773736,
-0.03869372606277466,
-0.050919316709041595,
-0.04068800061941147,
-0.007403296884149313,
0.062104228883981705,
0.07072226703166962,
-0.08550062775611877,
-0.10300061106681824,
-0.005464600399136543,
0.05054899677634239,
0.01169938500970602,
-0.043951600790023804,
0.018096856772899628,
0.03494584560394287,
0.016455961391329765,
-0.005494311451911926,
-0.005681003909558058,
0.06447509676218033,
0.020654670894145966,
-0.0625838115811348,
-0.021756349131464958,
-0.04406789690256119,
-0.017956567928195,
0.028213676065206528,
0.031776558607816696,
0.02972530759871006,
-0.0069245845079422,
0.0035604434087872505,
-0.004453351255506277,
0.02735283598303795,
-0.04379300773143768,
0.056713733822107315,
0.05177535116672516,
0.06069166213274002,
0.10065623372793198,
-0.020499851554632187,
0.07146500051021576,
0.09026770293712616,
0.03313695639371872,
-0.014148584567010403,
-0.051882725208997726,
-0.08183329552412033,
0.057893093675374985,
0.04022756218910217,
0.0027142770122736692,
-0.023302901536226273,
0.020237311720848083,
-0.05013137683272362,
0.014426841400563717,
0.03632980212569237,
-0.03879135847091675,
-0.010088532231748104,
-0.002409259555861354,
0.022955719381570816,
0.014789659529924393,
-0.007475234568119049,
-0.036803703755140305,
0.0013969781575724483,
0.045565225183963776,
-0.28864192962646484,
0.02720143087208271,
0.05926292762160301,
0.03149798512458801,
-0.016228528693318367,
0.035598207265138626,
0.007135331630706787,
-0.019815346226096153,
-0.07671882212162018,
0.013251231983304024,
-0.02948029153048992,
0.03133086487650871,
0.07073348015546799,
-0.010491331107914448,
0.01966356858611107,
0.002504149917513132,
0.014516197144985199,
0.0036083832383155823,
0.01730402186512947,
-0.03300950676202774,
0.01332666352391243,
-0.02573593519628048,
0.1647990494966507,
-0.01744060404598713,
-0.04355166107416153,
0.032889775931835175,
-0.009370021522045135,
0.04383092001080513,
-0.023841988295316696,
-0.008373380638659,
-0.005032366141676903,
-0.024896062910556793,
0.05946393683552742,
-0.04964049905538559,
0.04045291617512703,
0.039677638560533524,
-0.033816851675510406,
0.001962742069736123,
0.01312684640288353,
-0.0022081092465668917,
-0.008077201433479786,
0.019419919699430466,
-0.029912736266851425,
0.05847373232245445,
0.06631771475076675,
-0.0836765319108963,
0.013426519930362701,
-0.013016127049922943,
-0.003448644420132041,
0.011227271519601345,
-0.03589770197868347,
-0.03716911002993584,
0.023411089554429054,
0.014766814187169075,
0.03141472116112709,
-0.07829157263040543,
-0.0006572146085090935,
-0.022808494046330452,
-0.018561434000730515,
0.05371566116809845,
0.007807036861777306,
0.01907564513385296,
0.0037699067033827305,
0.06538920849561691,
-0.02359592355787754
] |
PMID:18689
|
[Use of an anxiolytic agent (lorazepam) in low doses in the treatment of anxiety states].
|
A nonblind clinical study was carried out by administering Lorazepam at a low dosage to patients with a light to moderate state of reactive anxiety. The drug was shown to be effective in the management of the target symptoms studied: anorexia, sleep induction, rhythm, and duration disturbances, depression, irritability and moodiness, fatigue, anxiety and tension, somatic anxiety, social adaptation. The results obtained are practically identical to those reported in the literature for higher doses. Moreover, low-dose Lorazepam was well tolerated and without undesired side-effects.
|
[Use of an anxiolytic agent (lorazepam) in low doses in the treatment of anxiety states]. A nonblind clinical study was carried out by administering Lorazepam at a low dosage to patients with a light to moderate state of reactive anxiety. The drug was shown to be effective in the management of the target symptoms studied: anorexia, sleep induction, rhythm, and duration disturbances, depression, irritability and moodiness, fatigue, anxiety and tension, somatic anxiety, social adaptation. The results obtained are practically identical to those reported in the literature for higher doses. Moreover, low-dose Lorazepam was well tolerated and without undesired side-effects.
|
[
-0.008696752600371838,
0.028910581022500992,
0.02274276129901409,
0.0501963272690773,
-0.012882071547210217,
0.0015912132803350687,
0.08532731980085373,
0.00042856918298639357,
-0.008868364617228508,
-0.09465798735618591,
0.057527102530002594,
0.0034337902907282114,
-0.010329270735383034,
0.0658988282084465,
-0.025253428146243095,
0.019959615543484688,
0.07282167673110962,
-0.03517631068825722,
0.004853548016399145,
-0.0041169277392327785,
-0.022715358063578606,
0.039646998047828674,
0.047288455069065094,
0.047379571944475174,
0.005282594822347164,
-0.015875108540058136,
-0.005402741488069296,
0.0005386010743677616,
-0.07748983055353165,
-0.12260248512029648,
0.054731667041778564,
-0.008045647293329239,
0.04226793721318245,
0.04986794665455818,
-0.06668087840080261,
0.018052129074931145,
-0.08661177009344101,
0.0489538349211216,
-0.05441849306225777,
0.005196548532694578,
0.10122448205947876,
0.02202516421675682,
-0.008336992934346199,
-0.041524045169353485,
-0.013706677593290806,
-0.08506227284669876,
-0.03784671053290367,
-0.02498180978000164,
0.0864645466208458,
-0.005466584116220474,
0.028769806027412415,
0.020064009353518486,
0.03536853939294815,
0.0309252068400383,
-0.044801123440265656,
-0.014148196205496788,
0.01635272614657879,
0.006484600715339184,
-0.06963738799095154,
0.029409941285848618,
-0.038765326142311096,
0.037716999650001526,
-0.12389587610960007,
0.012205556966364384,
-0.0021308220457285643,
-0.009555782191455364,
-0.026379544287919998,
-0.01566379889845848,
0.027238110080361366,
0.09501650184392929,
-0.06811172515153885,
0.016025204211473465,
0.04709337279200554,
0.04072839021682739,
0.06812658160924911,
-0.022541500627994537,
0.05088823661208153,
0.009216847829520702,
-0.03547005355358124,
-0.03965223208069801,
0.04715806618332863,
-0.0033174040727317333,
0.044851213693618774,
-0.07180403918027878,
0.017574232071638107,
-0.010127177461981773,
0.050539787858724594,
-0.00031154858879745007,
0.04181602969765663,
-0.0223336610943079,
0.02476482093334198,
-0.015577873215079308,
-0.007420922629535198,
-0.06594891846179962,
0.04733686521649361,
-0.03161057457327843,
-0.01689942181110382,
-0.02683032490313053,
-0.04474382847547531,
0.3897431492805481,
-0.0019809214863926172,
0.030854124575853348,
0.03222442418336868,
-0.027238676324486732,
-0.0233270563185215,
-0.03826741501688957,
0.01528810802847147,
-0.04893401637673378,
-0.016972487792372704,
0.02977583184838295,
0.005234973970800638,
-0.022787312045693398,
0.08997861295938492,
-0.01936992071568966,
0.009693808853626251,
-0.006861251778900623,
0.048393335193395615,
-0.02454950660467148,
0.022757822647690773,
0.06164040416479111,
-0.05297837033867836,
-0.06931217014789581,
0.02660560980439186,
-0.012779749929904938,
0.05707273632287979,
-0.02203184925019741,
-0.06616908311843872,
0.05939146503806114,
0.004649757873266935,
0.024013351649045944,
0.025717195123434067,
-0.05424899980425835,
0.029095182195305824,
0.05040150135755539,
0.027779877185821533,
-0.01693354919552803,
0.03969765454530716,
-0.009790422394871712,
0.021100252866744995,
-0.04309789091348648,
0.017580797895789146,
-0.026047565042972565,
0.05291202291846275,
-0.13445205986499786,
-0.06840753555297852,
0.11923059821128845,
0.031719762831926346,
0.01973099261522293,
-0.06299320608377457,
-0.019243251532316208,
-0.052866581827402115,
0.02968650870025158,
-0.028237896040081978,
0.0302705317735672,
-0.04724518209695816,
-0.008353794924914837,
0.005632460117340088,
0.017314845696091652,
-0.06083521246910095,
0.06525496393442154,
-0.01749264821410179,
-0.04732409492135048,
0.02360149845480919,
0.037756528705358505,
-0.05382903292775154,
0.013676432892680168,
-0.05537715554237366,
-0.026274271309375763,
-0.02772512659430504,
0.0068207839503884315,
0.03805753216147423,
-0.020523421466350555,
0.014177512377500534,
-0.029215015470981598,
-0.041936371475458145,
0.026316046714782715,
0.06065463274717331,
-0.01183425821363926,
-0.020105136558413506,
0.01661064848303795,
0.040270909667015076,
-0.0069869584403932095,
-0.007052360102534294,
0.005625714082270861,
-0.009693016298115253,
0.009086295031011105,
-0.005159664433449507,
0.04140929877758026,
-0.03191983699798584,
-0.02726946957409382,
-0.039220958948135376,
-0.029163798317313194,
-0.07902250438928604,
-0.042186543345451355,
-0.13123871386051178,
-0.03904903307557106,
-0.11845822632312775,
-0.031506702303886414,
-0.0027909507043659687,
0.011160344816744328,
0.08915098011493683,
0.0952145904302597,
0.053009241819381714,
0.024030128493905067,
0.03153708204627037,
0.09166190028190613,
0.010004772804677486,
-0.028716852888464928,
0.05629517510533333,
-0.022961774840950966,
0.06220746412873268,
0.024708611890673637,
0.013261765241622925,
0.04546908289194107,
-0.01999087817966938,
0.1177595779299736,
-0.0077496846206486225,
-0.003436783794313669,
0.030593257397413254,
-0.026545574888586998,
0.044902537018060684,
-0.015030471608042717,
-0.021220415830612183,
-0.2811434864997864,
-0.019174357876181602,
-0.023740706965327263,
-0.04658324643969536,
0.030497418716549873,
-0.056184329092502594,
-0.003551250323653221,
-0.03227933123707771,
-0.023385437205433846,
-0.028572924435138702,
0.033563464879989624,
-0.021068336442112923,
-0.05388439819216728,
0.0136342728510499,
0.013923676684498787,
0.03274080157279968,
0.07482630014419556,
-0.038902416825294495,
0.030894359573721886,
-0.034947607666254044,
-0.018862031400203705,
0.001197466510348022,
-0.01670600101351738,
-0.007901963777840137,
0.019476624205708504,
-0.0031577434856444597,
0.08811644464731216,
0.014599266462028027,
-0.037113066762685776,
-0.01977851241827011,
-0.0018039372516795993,
0.038808129727840424,
0.01622536964714527,
-0.11143522709608078,
0.016608022153377533,
0.018181918188929558,
0.06276349723339081,
-0.013934260234236717,
-0.02383006177842617,
-0.05127198249101639,
-0.06980837881565094,
0.01402844488620758,
0.019484523683786392,
-0.0846962034702301,
-0.10698169469833374,
0.0027757943607866764,
0.06841737776994705,
-0.009176567196846008,
0.0012875244719907641,
-0.02708296664059162,
-0.01057041808962822,
0.03378428518772125,
-0.007318960037082434,
0.03345312923192978,
0.002178560709580779,
-0.03152484819293022,
-0.12260489165782928,
-0.003044652286916971,
-0.005488087888807058,
-0.020067274570465088,
-0.006887272000312805,
-0.046501487493515015,
0.06447070091962814,
-0.01629510149359703,
-0.008106180466711521,
-0.029281916096806526,
0.0450601726770401,
-0.00990831945091486,
0.032941363751888275,
0.03290920332074165,
-0.006123813800513744,
0.09119521081447601,
-0.05188218876719475,
-0.01135291438549757,
0.0625092014670372,
0.014577803201973438,
-0.035841260105371475,
-0.010110481642186642,
-0.04697461798787117,
0.02904793992638588,
0.017166879028081894,
0.02324012853205204,
0.06382691115140915,
0.07195964455604553,
-0.015591162256896496,
-0.03860491141676903,
-0.009728857316076756,
-0.035531435161828995,
0.03591247275471687,
-0.06089328974485397,
0.026033714413642883,
-0.011424853466451168,
0.01805231161415577,
-0.006229367107152939,
0.008443518541753292,
0.02787088230252266,
-0.2460428774356842,
-0.028053291141986847,
-0.009309637360274792,
0.06897176057100296,
-0.025972507894039154,
0.0186383668333292,
0.030266638845205307,
0.026280097663402557,
-0.045534923672676086,
-0.02386777102947235,
0.028255585581064224,
-0.011415514163672924,
0.04374344274401665,
-0.0010896610328927636,
-0.011757522821426392,
-0.010426407679915428,
0.04016200080513954,
0.0008867767755873501,
0.0037113050930202007,
-0.04388200119137764,
0.02249635010957718,
0.027492454275488853,
0.08273666352033615,
0.015396223403513432,
0.026209041476249695,
0.0772475153207779,
0.022214340046048164,
-0.005193481221795082,
-0.06831571459770203,
0.03047078475356102,
-0.021422814577817917,
0.024180777370929718,
0.06335040181875229,
-0.015037107281386852,
0.023827627301216125,
-0.04321938008069992,
-0.08616415411233902,
-0.016508908942341805,
0.04208528622984886,
-0.010069613344967365,
0.006370736286044121,
0.04321612790226936,
0.05304867774248123,
0.030526353046298027,
0.05438574030995369,
-0.08072233945131302,
-0.012974709272384644,
-0.06383175402879715,
-0.040821053087711334,
-0.033799320459365845,
-0.0005308369291014969,
-0.0032288937363773584,
0.04572393745183945,
0.05080541595816612,
0.06729307770729065,
0.03150365874171257,
-0.043715350329875946,
-0.011532564647495747,
0.0261688269674778,
0.01446664147078991,
-0.02644643560051918,
-0.005778264719992876,
0.018100230023264885,
0.03960181772708893,
0.008523656986653805
] |
PMID:18698
|
Injuries to the ankle and foot in athletics.
|
Accurate diagnosis in ligament and ligament-bone injuries of the foot and ankle demands a knowledge of the anatomy and mechanics of the foot and ankle, a good history of the mechanism of injury, clinical examination including fingertip palpation and assessment of stability, and adequate x-ray studies, perferably in the deformed position, with stress films and arthrograms in selected cases. By making an accurate diagnosis, early proper treatment is instituted, the athlete is restored to health sooner, and, almost as important, the athlete and the coach get a more realistic prognosis regarding the end result and duration of disability.
|
Injuries to the ankle and foot in athletics. Accurate diagnosis in ligament and ligament-bone injuries of the foot and ankle demands a knowledge of the anatomy and mechanics of the foot and ankle, a good history of the mechanism of injury, clinical examination including fingertip palpation and assessment of stability, and adequate x-ray studies, perferably in the deformed position, with stress films and arthrograms in selected cases. By making an accurate diagnosis, early proper treatment is instituted, the athlete is restored to health sooner, and, almost as important, the athlete and the coach get a more realistic prognosis regarding the end result and duration of disability.
|
[
-0.012193738482892513,
-0.005772055126726627,
0.09508943557739258,
-0.012765223160386086,
0.06763894110918045,
0.00411641551181674,
0.05692501738667488,
0.08786587417125702,
-0.016228720545768738,
-0.01610521972179413,
0.013243582099676132,
-0.05551745742559433,
0.011203284375369549,
0.02795540727674961,
0.00775523204356432,
0.003707884345203638,
0.013427902944386005,
0.07432931661605835,
0.010639334097504616,
0.021166589111089706,
0.013576834462583065,
0.012293488718569279,
-0.05350593477487564,
0.0040184613317251205,
0.01223035529255867,
0.02911708503961563,
-0.009056322276592255,
0.032489657402038574,
-0.0011604399187490344,
-0.0953257828950882,
-0.040492597967386246,
0.0013449537800624967,
-0.0317554771900177,
-0.007814381271600723,
0.016908345744013786,
-0.05642058327794075,
-0.1058083176612854,
0.012300726026296616,
0.06631024181842804,
0.05988038703799248,
0.040287476032972336,
0.04869907721877098,
-0.0019195305649191141,
-0.03661565110087395,
0.06887249648571014,
0.04106804355978966,
0.001296972157433629,
0.041346609592437744,
0.08359423279762268,
0.022047029808163643,
-0.0487201027572155,
0.07301925867795944,
0.03471739962697029,
0.029312297701835632,
0.029252424836158752,
0.0018229192355647683,
0.037757083773612976,
0.017892971634864807,
0.07179905474185944,
0.03101721592247486,
0.04254082590341568,
0.04288417845964432,
-0.113007552921772,
0.07647383958101273,
-0.03789263963699341,
-0.0019413838163018227,
-0.0163835771381855,
-0.03081369586288929,
0.04095681756734848,
0.06271541863679886,
-0.014513278380036354,
0.028938300907611847,
0.0707114040851593,
0.05958861485123634,
-0.04749172553420067,
-0.07508488744497299,
0.002282076748088002,
-0.007141228299587965,
0.022775473073124886,
0.027478760108351707,
-0.018322402611374855,
-0.0301726832985878,
0.014904328621923923,
0.01594969443976879,
-0.04327463358640671,
-0.05223885551095009,
-0.0028307070024311543,
0.00006720076635247096,
0.04543108865618706,
0.020764263346791267,
-0.015836268663406372,
-0.024906855076551437,
-0.05194613337516785,
0.06085106357932091,
0.00009328267333330587,
0.031507283449172974,
-0.04616646468639374,
-0.019351495429873466,
-0.07297278195619583,
0.38330987095832825,
-0.03534811735153198,
0.026049649342894554,
0.044101472944021225,
0.0039395177736878395,
-0.02005455084145069,
-0.09193326532840729,
0.0284112598747015,
0.029736507683992386,
-0.0512164831161499,
0.010487707331776619,
0.09063997119665146,
-0.01663660816848278,
0.0035963321570307016,
0.011501309461891651,
-0.06208058446645737,
0.04589701443910599,
0.001007547602057457,
0.01294077467173338,
0.032776083797216415,
0.02114671654999256,
0.030034897848963737,
-0.024322593584656715,
0.009017367847263813,
-0.009690609760582447,
-0.057953841984272,
-0.0355001799762249,
0.024568073451519012,
0.05758491903543472,
0.07228983938694,
0.0340203158557415,
-0.01046735979616642,
-0.08588533848524094,
-0.028842870146036148,
-0.021388791501522064,
0.03293104097247124,
0.027377735823392868,
0.005647481884807348,
-0.04599486291408539,
-0.06031782925128937,
0.0730796605348587,
-0.03301781788468361,
-0.12036384642124176,
0.042352598160505295,
-0.017220769077539444,
-0.04032142832875252,
0.12352656573057175,
-0.05230353772640228,
0.05682038888335228,
0.021635478362441063,
-0.032924432307481766,
0.03061317279934883,
-0.05102742835879326,
-0.06989530473947525,
0.015956297516822815,
-0.022778790444135666,
-0.043543655425310135,
0.01856284774839878,
-0.03296410292387009,
-0.01522877812385559,
-0.03880508616566658,
-0.009506288915872574,
-0.025300998240709305,
-0.04491029679775238,
0.05605044588446617,
0.010769734159111977,
-0.04032473638653755,
0.0043938616290688515,
0.042174793779850006,
-0.003758822102099657,
-0.0016179558588191867,
0.005527889356017113,
-0.021800175309181213,
0.08192697167396545,
-0.037620093673467636,
-0.005956545472145081,
0.04715096205472946,
-0.036480292677879333,
-0.032870855182409286,
0.006716321688145399,
-0.012186238542199135,
0.11302199959754944,
-0.032755669206380844,
-0.012463240884244442,
0.028813589364290237,
0.018585555255413055,
0.04536932706832886,
-0.1135224997997284,
0.0050048320554196835,
0.0951535627245903,
0.01877071149647236,
-0.045586343854665756,
-0.050376616418361664,
-0.04284936562180519,
-0.06865710020065308,
0.02069823071360588,
0.016145436093211174,
-0.03174832463264465,
-0.006499715615063906,
-0.0756952166557312,
-0.0030094704125076532,
0.010921276174485683,
0.046592969447374344,
0.0005494579672813416,
0.0166498813778162,
-0.003519392339512706,
0.04127193242311478,
-0.025452537462115288,
-0.02164565771818161,
-0.011304599232971668,
0.021630924195051193,
-0.04468275606632233,
0.010386431589722633,
-0.0241636261343956,
-0.0740930438041687,
-0.010119044221937656,
-0.02563602849841118,
-0.0009060612646862864,
0.022201694548130035,
-0.033285655081272125,
0.02944103069603443,
0.00633215019479394,
0.013099154457449913,
0.002877075457945466,
-0.3069820702075958,
-0.04767129197716713,
0.02406729944050312,
0.0693042129278183,
0.030784383416175842,
0.027068989351391792,
-0.01097184605896473,
-0.048477549105882645,
-0.026252616196870804,
-0.029127519577741623,
0.06564199924468994,
0.026280896738171577,
-0.01276843436062336,
-0.101364865899086,
-0.021743254736065865,
0.008123613893985748,
0.03020288236439228,
-0.017420025542378426,
-0.028009923174977303,
0.005117356311529875,
-0.03333694860339165,
0.04667196422815323,
0.00003380455746082589,
-0.051542673259973526,
0.03234213963150978,
-0.06518377363681793,
0.07375479489564896,
0.05647747218608856,
-0.027434280142188072,
-0.10925791412591934,
-0.014124571345746517,
-0.02795345149934292,
-0.012949335388839245,
-0.06786774843931198,
0.043352484703063965,
-0.0031754160299897194,
-0.03159390762448311,
-0.011688312515616417,
-0.013616399839520454,
-0.04738541692495346,
0.011496886610984802,
-0.01808512769639492,
0.03449157997965813,
0.017166759818792343,
-0.0381724089384079,
-0.004591559991240501,
-0.00035515284980647266,
-0.03721985965967178,
0.020602678880095482,
0.028624165803194046,
0.019970640540122986,
-0.013812764547765255,
0.07182987779378891,
-0.008731701411306858,
-0.0480521023273468,
-0.025726022198796272,
-0.027045510709285736,
-0.010655976831912994,
-0.06180969253182411,
-0.05392647534608841,
0.024611275643110275,
-0.007619412615895271,
0.05308527126908302,
-0.005131252575665712,
0.012938897125422955,
0.03254213556647301,
-0.017501303926110268,
-0.020501378923654556,
-0.001818640623241663,
-0.028223197907209396,
-0.010821823962032795,
0.027249960228800774,
0.02387063018977642,
-0.019591886550188065,
0.03112752176821232,
0.05446941778063774,
0.08096879720687866,
-0.042977381497621536,
0.026071695610880852,
0.02669101022183895,
0.06845759600400925,
-0.016714129596948624,
0.06451183557510376,
0.0096244802698493,
0.02247442863881588,
-0.021536625921726227,
0.006789887323975563,
-0.004785621538758278,
0.010232384316623211,
-0.02481035701930523,
-0.0011505386792123318,
0.016411948949098587,
-0.018043875694274902,
-0.011121713556349277,
-0.057888325303792953,
0.03237268328666687,
-0.24326477944850922,
0.012736091390252113,
0.06788884848356247,
0.007824288681149483,
-0.03898926079273224,
-0.060228366404771805,
0.03955889865756035,
0.04133172705769539,
-0.02375469170510769,
-0.0023349011316895485,
0.009994404390454292,
-0.05800842493772507,
0.03933428227901459,
-0.0377332866191864,
-0.07340240478515625,
0.03745195269584656,
0.14301498234272003,
-0.08360280841588974,
0.06755959242582321,
-0.041377417743206024,
-0.017076287418603897,
-0.0015595871955156326,
0.0945102721452713,
0.00965031236410141,
0.04390731081366539,
0.08532936871051788,
0.009424159303307533,
0.014417692087590694,
-0.09097347408533096,
-0.016771100461483,
0.030753077939152718,
-0.0494186133146286,
-0.07041803747415543,
0.05946500599384308,
-0.038344353437423706,
0.03084501251578331,
0.020491624251008034,
0.05033824220299721,
-0.014061475172638893,
-0.01309776958078146,
0.05479423329234123,
-0.031042788177728653,
-0.015796251595020294,
-0.047294676303863525,
0.08672108501195908,
0.022496994584798813,
-0.023362163454294205,
-0.014234393835067749,
-0.007900223135948181,
-0.015708932653069496,
0.017413664609193802,
-0.03180272504687309,
-0.02137460932135582,
-0.022286025807261467,
-0.013349637389183044,
-0.009446423500776291,
0.04663435369729996,
-0.004130077548325062,
0.03633958101272583,
-0.07577625662088394,
0.0004893372533842921,
-0.040797844529151917,
-0.07747454196214676,
0.08095844089984894,
0.019255464896559715
] |
PMID:18701
|
Polyarteritis nodosa in older children.
|
Polyarteritis in the older child is thought to be a rare disease. This study describes 11 children, 3 to 12 years of age, with polyarteritis seen over a five-year period. Fever, abdominal pain, hypertension, and leukocytosis were found in almost all. Renal disease occurred in eight. Examination of muscle, gut, or kidney tissue was an effective means of diagnosis. The pathological changes were the same as those seen in adults. There seemed to be an association between polyarteritis and group A streptococcal infection. Ten patients had a salutary response to high-dose prednisone administration.
|
Polyarteritis nodosa in older children. Polyarteritis in the older child is thought to be a rare disease. This study describes 11 children, 3 to 12 years of age, with polyarteritis seen over a five-year period. Fever, abdominal pain, hypertension, and leukocytosis were found in almost all. Renal disease occurred in eight. Examination of muscle, gut, or kidney tissue was an effective means of diagnosis. The pathological changes were the same as those seen in adults. There seemed to be an association between polyarteritis and group A streptococcal infection. Ten patients had a salutary response to high-dose prednisone administration.
|
[
-0.03561880439519882,
-0.030527114868164062,
0.06163959205150604,
-0.018806129693984985,
-0.004895288497209549,
-0.015712793916463852,
0.052517108619213104,
0.032093845307826996,
-0.06522638350725174,
-0.034396298229694366,
0.0476103313267231,
-0.03677988424897194,
0.010150776244699955,
0.00992986373603344,
-0.07410421222448349,
0.009086944162845612,
0.015488021075725555,
-0.0050565083511173725,
-0.055451031774282455,
0.04745028167963028,
-0.037231408059597015,
0.03178653120994568,
-0.0003389272897038609,
-0.05209970846772194,
0.03130531311035156,
-0.004471848253160715,
0.07082762569189072,
-0.024786997586488724,
-0.022161727771162987,
-0.1268402338027954,
-0.01124504767358303,
-0.05893748626112938,
-0.03800777718424797,
-0.019251303747296333,
-0.011374401859939098,
0.015181205235421658,
-0.011403371579945087,
0.05326906591653824,
-0.029217684641480446,
0.03035152144730091,
-0.0043174875900149345,
0.001075415755622089,
-0.006821623537689447,
-0.010975181125104427,
-0.06610624492168427,
-0.013149953447282314,
-0.055960800498723984,
0.0027768115978688,
0.16658101975917816,
0.0535116083920002,
-0.07522550225257874,
0.01136004738509655,
0.010262183845043182,
0.10031387209892273,
-0.006614496000111103,
0.010086244903504848,
-0.035268668085336685,
-0.05013126879930496,
0.04146437719464302,
0.0072335852310061455,
0.06087641417980194,
0.003353270934894681,
-0.08921199291944504,
0.10298972576856613,
0.012959131971001625,
0.02851375937461853,
0.006107269320636988,
0.008659645915031433,
-0.01417371816933155,
-0.010736868716776371,
-0.011731489561498165,
0.06635726243257523,
-0.024884432554244995,
0.08165799826383591,
-0.06740733236074448,
0.017867211252450943,
-0.05474628880620003,
-0.04725603759288788,
0.04720818251371384,
0.03698566183447838,
0.010031458921730518,
0.09372834116220474,
-0.020456265658140182,
-0.008501061238348484,
-0.00028549775015562773,
-0.03825930505990982,
-0.03109137900173664,
-0.04714169725775719,
0.004230383783578873,
0.008928635157644749,
-0.054346393793821335,
0.006810441613197327,
-0.04943317919969559,
0.0072568850591778755,
-0.05969801917672157,
0.02253001183271408,
0.034273289144039154,
-0.008934011682868004,
-0.04078811779618263,
0.3767853081226349,
-0.04128805175423622,
-0.04115496575832367,
0.009926440194249153,
0.02997666969895363,
-0.027515174821019173,
-0.0034415374975651503,
0.02898520790040493,
-0.019982855767011642,
-0.007743752561509609,
0.007773158606141806,
-0.048544712364673615,
0.012608649209141731,
0.058447957038879395,
-0.005515191704034805,
-0.022197768092155457,
-0.007693971041589975,
0.002631032606586814,
0.014753189869225025,
-0.05073726177215576,
0.07239808142185211,
-0.01146412268280983,
-0.04126816615462303,
0.003951324615627527,
0.010621431283652782,
0.06540994346141815,
0.023734882473945618,
0.08694912493228912,
0.060724832117557526,
0.05688582360744476,
0.023844130337238312,
0.058537207543849945,
-0.04140997678041458,
-0.06804633885622025,
-0.03381258621811867,
0.06581419706344604,
0.008719599805772305,
0.0034995314199477434,
0.023532351478934288,
0.015281839296221733,
-0.01895575225353241,
-0.029126809909939766,
-0.06787305325269699,
-0.05103081464767456,
-0.007975027896463871,
-0.05371572822332382,
0.09255043417215347,
0.02283385954797268,
0.036949336528778076,
-0.047756653279066086,
0.009003665298223495,
-0.0019006646471098065,
0.07250656187534332,
-0.00831417366862297,
0.01788559928536415,
0.02820916660130024,
0.006910702213644981,
-0.07014471292495728,
0.012750380672514439,
0.03492116183042526,
-0.0422256700694561,
0.011753997765481472,
-0.023985298350453377,
-0.02579551935195923,
-0.011045992374420166,
0.013401702046394348,
-0.013884179294109344,
-0.02043399028480053,
-0.03416653722524643,
-0.01999213919043541,
0.021538134664297104,
0.0061222948133945465,
0.05969429016113281,
0.042130012065172195,
0.023130429908633232,
-0.010951356962323189,
0.025211777538061142,
0.023904211819171906,
-0.0017945201834663749,
0.032519493252038956,
-0.007582280319184065,
0.0012474149698391557,
0.038118235766887665,
0.009098214097321033,
0.05354481562972069,
-0.022552644833922386,
-0.006043317727744579,
-0.02581622451543808,
0.013984733261168003,
0.04272431507706642,
-0.01189559418708086,
0.010617950931191444,
-0.014436648227274418,
-0.08730880171060562,
-0.0010943562956526875,
-0.018579788506031036,
-0.025862084701657295,
-0.04675929993391037,
-0.001896824804134667,
-0.010525450110435486,
-0.03525906801223755,
0.04231292009353638,
-0.02697858028113842,
0.034667905420064926,
0.02666759304702282,
-0.022878721356391907,
-0.010176323354244232,
0.02019461803138256,
-0.0277565885335207,
-0.027737224474549294,
0.005451123230159283,
-0.025877172127366066,
0.035572078078985214,
0.006104157771915197,
-0.02072606049478054,
-0.019023224711418152,
0.05793118104338646,
-0.08249302208423615,
-0.00911020953208208,
-0.017484333366155624,
0.02752026729285717,
0.056626494973897934,
-0.012278708629310131,
0.036701589822769165,
-0.2892238199710846,
0.016889574006199837,
0.0067254891619086266,
-0.045112449675798416,
0.022628795355558395,
0.003927553538233042,
0.01576170139014721,
0.018590815365314484,
-0.01900487393140793,
-0.008719495497643948,
-0.013613557443022728,
0.1032671257853508,
-0.019393514841794968,
-0.05100921168923378,
-0.00498582748696208,
0.07268112897872925,
0.08140212297439575,
0.015237834304571152,
0.02174888551235199,
0.005585282575339079,
-0.01154467836022377,
-0.07541175931692123,
0.004549861885607243,
0.06686632335186005,
0.09240639954805374,
0.022762823849916458,
0.1536669284105301,
-0.024259120225906372,
-0.07372606545686722,
-0.08340419828891754,
0.10856600850820541,
-0.005570215173065662,
-0.026804236695170403,
-0.008607114665210247,
0.04686708003282547,
-0.008992182090878487,
-0.018217047676444054,
-0.03575741872191429,
-0.034866493195295334,
-0.03193337842822075,
-0.07108927518129349,
-0.008787324652075768,
-0.015990594401955605,
0.04551088809967041,
-0.04147249832749367,
0.006109115667641163,
0.041260238736867905,
0.04163573309779167,
0.012655201368033886,
0.04921186342835426,
-0.009140023030340672,
-0.028400734066963196,
0.00741963554173708,
0.027228405699133873,
-0.013379945419728756,
-0.0322495773434639,
0.017090197652578354,
0.009374838322401047,
-0.0730825662612915,
-0.0007104286341927946,
-0.03613955155014992,
0.019378751516342163,
0.06053374335169792,
-0.06369393318891525,
-0.027371255680918694,
0.027709005400538445,
0.026123564690351486,
-0.04005150869488716,
0.02722330577671528,
0.0006410963833332062,
-0.0325455367565155,
0.09403109550476074,
-0.048034992069005966,
-0.02962179109454155,
0.056243833154439926,
0.04113204777240753,
0.013519277796149254,
0.007166826166212559,
-0.024366121739149094,
0.052089039236307144,
0.050555482506752014,
-0.13543105125427246,
-0.011550109833478928,
0.03071637637913227,
0.021035149693489075,
0.0005187593633309007,
-0.0536205880343914,
0.0006101931212469935,
-0.06910361349582672,
-0.07936374843120575,
-0.0013758208369836211,
-0.03188732638955116,
-0.0527852438390255,
0.022459615021944046,
-0.02118060551583767,
0.005508326925337315,
-0.2668421268463135,
0.041531041264534,
0.018753036856651306,
-0.0098708625882864,
-0.007236636709421873,
0.02636285312473774,
-0.011450421996414661,
-0.040661029517650604,
0.012460418045520782,
0.027558060362935066,
0.06135581061244011,
0.01145657617598772,
0.06362009793519974,
-0.016905266791582108,
-0.07994434237480164,
0.042328912764787674,
0.011187315918505192,
0.0456172414124012,
0.008642779663205147,
-0.10933732241392136,
0.007416269741952419,
-0.019335981458425522,
0.11068258434534073,
0.0627085492014885,
-0.044567082077264786,
0.02493571676313877,
-0.03483332321047783,
0.07972288131713867,
-0.0553472526371479,
-0.0197428148239851,
-0.02900427021086216,
-0.02235843613743782,
0.04386912286281586,
0.020969998091459274,
0.06356953829526901,
0.07095708698034286,
-0.05022707208991051,
0.007909219712018967,
0.011160078458487988,
0.020715178921818733,
-0.0016064696246758103,
-0.006147649139165878,
-0.04566466063261032,
-0.018779203295707703,
-0.023404214531183243,
0.05693841353058815,
0.019019735977053642,
-0.03505958244204521,
0.06496686488389969,
-0.006462122313678265,
-0.021016964688897133,
-0.060690123587846756,
0.009657627902925014,
0.03408094868063927,
0.06481436640024185,
0.009508518502116203,
-0.024818263947963715,
-0.08420998603105545,
0.008112957701086998,
-0.07082920521497726,
0.012705237604677677,
0.03987708315253258,
-0.06703711301088333,
0.10752442479133606,
0.024386830627918243
] |
PMID:18703
|
Properties of the HCO-3-stimulated Mg2+ -ATPase activity in red cell membranes.
|
The HCO-3-stimulated Mg2+ -ATPase activity in red cell ghost fragments was investigated. Increasing the HCO-3 concentration in the incubation medium resulted in increased ATPase activity. NaHCO3 appeared to be more effective than KHCO3 in this regard. The ATPase activities were slightly stimulated by increases in ionic strength and utilized ITP almost as readily as ATP. A Mg/ATP ratio of 1.0 and a pH of 7.6 yielded maximum activity. These properties are of interest since the present enzyme is the only unquestionable instance where a HCO-3 ATPase is located in the surface membrane of a cell.
|
Properties of the HCO-3-stimulated Mg2+ -ATPase activity in red cell membranes. The HCO-3-stimulated Mg2+ -ATPase activity in red cell ghost fragments was investigated. Increasing the HCO-3 concentration in the incubation medium resulted in increased ATPase activity. NaHCO3 appeared to be more effective than KHCO3 in this regard. The ATPase activities were slightly stimulated by increases in ionic strength and utilized ITP almost as readily as ATP. A Mg/ATP ratio of 1.0 and a pH of 7.6 yielded maximum activity. These properties are of interest since the present enzyme is the only unquestionable instance where a HCO-3 ATPase is located in the surface membrane of a cell.
|
[
-0.08948986977338791,
-0.05054623633623123,
-0.02507859095931053,
0.06144044175744057,
0.0417356863617897,
-0.02532185986638069,
0.06680820882320404,
0.05190947279334068,
-0.06449297815561295,
-0.0109872343018651,
0.04660196229815483,
-0.05580073595046997,
-0.04776699095964432,
0.017791537567973137,
0.03853552043437958,
-0.04129794239997864,
0.013537365011870861,
0.02074696682393551,
-0.06631910800933838,
-0.012349702417850494,
-0.007052618078887463,
0.0036203046329319477,
0.0026140641421079636,
0.025229522958397865,
0.05660061538219452,
0.0512838140130043,
-0.0024067754857242107,
-0.006495976820588112,
-0.10156265646219254,
-0.15352940559387207,
0.01678675040602684,
-0.05472449213266373,
0.020063897594809532,
-0.007064627017825842,
-0.015409806743264198,
0.031813181936740875,
-0.053879883140325546,
0.020983824506402016,
-0.06474382430315018,
0.03659943491220474,
0.051906950771808624,
0.017693616449832916,
0.015103870071470737,
0.048433490097522736,
-0.04790773615241051,
-0.0506991446018219,
-0.029461927711963654,
0.03444637730717659,
0.09100241959095001,
0.030399398878216743,
0.0029288579244166613,
0.06387577205896378,
0.029045168310403824,
0.009614694863557816,
0.06571414321660995,
-0.031453970819711685,
-0.02867731638252735,
0.03350643813610077,
-0.01335148885846138,
-0.005915121175348759,
0.04438749700784683,
0.02501773275434971,
-0.11634818464517593,
0.00423913961276412,
0.05540855601429939,
-0.01994340494275093,
-0.030899791046977043,
-0.012287558056414127,
0.007910038344562054,
0.06174888089299202,
-0.054197538644075394,
0.010725254192948341,
-0.04991001635789871,
0.028184453025460243,
0.007739362772554159,
-0.029705997556447983,
-0.007121944800019264,
-0.0005164392059668899,
0.011301601305603981,
-0.01653103716671467,
-0.03132058307528496,
-0.08508122712373734,
-0.007698326837271452,
0.01663070358335972,
-0.0018896933179348707,
0.0019496059976518154,
0.03822455182671547,
-0.04091501608490944,
-0.03393315151333809,
0.0009328130399808288,
0.011266354471445084,
-0.014502156525850296,
-0.0706048458814621,
-0.02190634235739708,
-0.10309282690286636,
0.06336963176727295,
0.025233455002307892,
0.04496239498257637,
-0.02704460732638836,
0.38960856199264526,
0.03440093249082565,
0.030868571251630783,
-0.03991018235683441,
0.012757276184856892,
-0.003973870538175106,
-0.0088192168623209,
0.00951396208256483,
0.025273868814110756,
-0.019410645589232445,
-0.0005047153099440038,
0.08345979452133179,
0.015724051743745804,
-0.007634369190782309,
-0.06491228193044662,
-0.021884363144636154,
0.012598074041306973,
0.04120427742600441,
-0.0017035279888659716,
0.001987647730857134,
0.07398275285959244,
-0.03526647761464119,
0.009596421383321285,
0.06260253489017487,
0.006858714856207371,
0.07094688713550568,
-0.011957253329455853,
-0.025151634588837624,
0.0909280776977539,
0.05228423699736595,
0.02951059490442276,
0.019220132380723953,
0.022411372512578964,
-0.06660854071378708,
-0.016373367980122566,
-0.008561816066503525,
-0.005827766843140125,
0.038200948387384415,
-0.03515590354800224,
0.06332145631313324,
-0.02586788311600685,
-0.031951654702425,
-0.02123391628265381,
-0.06922714412212372,
-0.0794479250907898,
-0.0497666634619236,
0.03338952362537384,
-0.014101549051702023,
0.061623066663742065,
-0.00824662484228611,
0.0009482590830884874,
0.03674434870481491,
0.04593714326620102,
-0.00012285794946365058,
0.045553043484687805,
-0.014054830186069012,
-0.04088935628533363,
0.007222750689834356,
-0.020252054557204247,
0.007086859550327063,
-0.02059856802225113,
-0.1283542960882187,
-0.06231521815061569,
0.043215200304985046,
0.0792878270149231,
0.006595299579203129,
-0.018564539030194283,
-0.03389369696378708,
-0.015760483220219612,
-0.025281861424446106,
0.011780424043536186,
-0.014008735306560993,
0.038706280291080475,
-0.042935606092214584,
-0.04681728407740593,
-0.008063806220889091,
0.060865290462970734,
-0.010923526249825954,
-0.009592533111572266,
0.07588402926921844,
-0.031229056417942047,
-0.003934875596314669,
0.0001997792423935607,
-0.02225843258202076,
0.007069802843034267,
0.0051446473225951195,
-0.0056536258198320866,
-0.0341000072658062,
0.061175696551799774,
0.0009738157386891544,
0.08878806233406067,
-0.045103613287210464,
-0.02065443992614746,
-0.06516211479902267,
0.021108120679855347,
0.022411521524190903,
-0.07501358538866043,
-0.0019028416136279702,
-0.0077072312124073505,
-0.03131856769323349,
-0.030942074954509735,
0.06992967426776886,
0.02919004298746586,
-0.011848032474517822,
0.048724401742219925,
-0.036221619695425034,
0.04089002311229706,
-0.05261501669883728,
0.06850064545869827,
0.01754891499876976,
0.005142121575772762,
-0.03468978404998779,
0.05734656751155853,
-0.05770505219697952,
-0.003171754302456975,
-0.04910208284854889,
0.020013069733977318,
0.004446874372661114,
0.017386171966791153,
0.05087459087371826,
0.03832279145717621,
0.030950089916586876,
-0.051996245980262756,
-0.07732041925191879,
-0.3024755120277405,
-0.036852285265922546,
-0.020046601071953773,
-0.0098070427775383,
0.07746431231498718,
0.016714761033654213,
0.016482975333929062,
0.01936647854745388,
0.017742469906806946,
-0.1063772514462471,
0.027371056377887726,
0.02008768357336521,
-0.011767144314944744,
-0.054165907204151154,
0.006564792711287737,
0.014984525740146637,
-0.026069942861795425,
-0.03819910064339638,
0.017962481826543808,
0.02154705673456192,
0.10392510145902634,
0.026696685701608658,
0.009830292314291,
0.012080302461981773,
0.0710146576166153,
-0.01936679147183895,
0.07187255471944809,
0.011246999725699425,
0.02405543066561222,
-0.017540965229272842,
0.023626381531357765,
-0.024319009855389595,
-0.04732336476445198,
-0.07927259057760239,
0.01614302024245262,
-0.008413772098720074,
0.019052628427743912,
-0.02843105047941208,
0.0032021943479776382,
-0.04196615889668465,
-0.006445764563977718,
0.0227779783308506,
-0.023918094113469124,
0.011653265915811062,
0.011510253883898258,
-0.005271336529403925,
0.07105787098407745,
0.060160089284181595,
0.02720731869339943,
-0.020879002287983894,
0.09614066779613495,
0.03370403125882149,
-0.03120676428079605,
-0.046850383281707764,
-0.016204215586185455,
-0.014890303835272789,
-0.01180932205170393,
0.051771849393844604,
-0.11418471485376358,
0.022023364901542664,
-0.020306820049881935,
0.006127143278717995,
-0.034973811358213425,
0.05228157341480255,
-0.07342559099197388,
0.00026704091578722,
0.05980462580919266,
-0.006425442174077034,
0.04612014815211296,
0.015719735994935036,
0.014620904810726643,
0.0969744473695755,
0.00999266654253006,
-0.019167115911841393,
0.05623432993888855,
0.04703453928232193,
-0.03618679940700531,
-0.04496350511908531,
0.004542329348623753,
0.03769085928797722,
0.013898725621402264,
-0.012425039894878864,
0.08512241393327713,
-0.00118082738481462,
-0.014382585883140564,
0.053515657782554626,
-0.007822186686098576,
-0.01558678224682808,
-0.025369839742779732,
-0.012095867656171322,
0.0006129754474386573,
-0.044413723051548004,
-0.07014970481395721,
-0.06307701766490936,
0.02804536372423172,
0.002243221737444401,
-0.2663860321044922,
-0.039260946214199066,
0.01222392451018095,
0.02481415867805481,
-0.04096205532550812,
-0.0024629842955619097,
0.02834957465529442,
-0.016897663474082947,
-0.03210019692778587,
0.017798101529479027,
0.038088295608758926,
0.047530315816402435,
0.05182699114084244,
0.08334961533546448,
-0.023055607452988625,
-0.006717904936522245,
0.01381223089993,
-0.07012894749641418,
-0.005753583274781704,
-0.025651119649410248,
0.028728947043418884,
0.00020298869640100747,
0.11835349351167679,
-0.06213711202144623,
0.02071247436106205,
0.05393414944410324,
-0.0035774889402091503,
0.012653404846787453,
-0.07420141994953156,
-0.028243273496627808,
-0.01299925148487091,
0.0460190549492836,
0.02061963453888893,
0.011731800623238087,
0.03416180983185768,
0.015530687756836414,
0.028592733666300774,
0.01990172080695629,
-0.057372137904167175,
0.027233347296714783,
-0.019669754430651665,
-0.0019294415833428502,
-0.08963847905397415,
0.006642411462962627,
0.06174948439002037,
-0.03224252164363861,
-0.06180607154965401,
-0.0743614211678505,
0.025903984904289246,
-0.028272384777665138,
-0.045236602425575256,
-0.033888667821884155,
0.028161827474832535,
-0.00440455786883831,
0.005057559348642826,
0.05544648692011833,
-0.041283607482910156,
0.01131370384246111,
-0.00430362718179822,
0.02579469420015812,
0.023085597902536392,
-0.023265235126018524,
0.06893368065357208,
0.06698446720838547,
0.028273966163396835
] |
PMID:18728
|
Methylprednisolone pulse therapy in the treatment of polyarteritis nodosa.
|
Two cases of lung granulomata associated with "crescentic" glomerulonephritis both in a clinical setting of polyarteritis nodosa were treated with high doses of intravenous methylprednisolone ("pulse therapy") in single injections of 30 mg/kg body-weight. In the first case rapidly progressive glomerulonephritis with 100% crescents was arrested and followed by improvement of renal function from a creatinine clearance of 5 ml/min to 30 ml/min; in the second case multiple lung granulomata of 8 months' standing, unresponsive to oral steroids, disappeared 8 days after treatment with high dosage intravenous methylprednisolone. The use of "pulse therapy" with methylprednisolone is advocated, not only in such cases where arteritis is known or suspected, but also in radidly progressive glomerulonephritis associated with crescents.
|
Methylprednisolone pulse therapy in the treatment of polyarteritis nodosa. Two cases of lung granulomata associated with "crescentic" glomerulonephritis both in a clinical setting of polyarteritis nodosa were treated with high doses of intravenous methylprednisolone ("pulse therapy") in single injections of 30 mg/kg body-weight. In the first case rapidly progressive glomerulonephritis with 100% crescents was arrested and followed by improvement of renal function from a creatinine clearance of 5 ml/min to 30 ml/min; in the second case multiple lung granulomata of 8 months' standing, unresponsive to oral steroids, disappeared 8 days after treatment with high dosage intravenous methylprednisolone. The use of "pulse therapy" with methylprednisolone is advocated, not only in such cases where arteritis is known or suspected, but also in radidly progressive glomerulonephritis associated with crescents.
|
[
-0.03536200895905495,
-0.02480372041463852,
0.02606482431292534,
-0.02274841070175171,
-0.008066503331065178,
-0.033870287239551544,
0.04402206093072891,
-0.014598977752029896,
0.006317631341516972,
-0.030753741040825844,
-0.0009169781114906073,
-0.027765341103076935,
0.03557656705379486,
0.010617339983582497,
-0.04955672845244408,
0.034123606979846954,
0.02253413014113903,
-0.012454141862690449,
-0.04154111072421074,
0.0006829845369793475,
0.04355785995721817,
0.014763792045414448,
0.016250474378466606,
0.011620397679507732,
0.033124055713415146,
-0.033554937690496445,
0.06317641586065292,
-0.030905330553650856,
-0.03625766932964325,
-0.18637344241142273,
0.0024754449259489775,
0.01987960934638977,
-0.06062191724777222,
0.021217409521341324,
-0.027160055935382843,
-0.043884146958589554,
-0.0447913333773613,
-0.017677009105682373,
-0.042783088982105255,
0.044669438153505325,
0.04509790986776352,
0.04908546060323715,
-0.0008682153420522809,
0.023654503747820854,
-0.020625555887818336,
0.029651040211319923,
-0.06836153566837311,
-0.026922820135951042,
0.15053878724575043,
-0.0010199378011748195,
0.010151908732950687,
-0.004044950939714909,
0.03426322713494301,
0.08133102208375931,
-0.05002736300230026,
-0.011313039809465408,
0.017491107806563377,
0.0004906756803393364,
0.015192525461316109,
0.005268458276987076,
0.04555037245154381,
0.03789264336228371,
-0.11571970582008362,
0.07426860928535461,
0.008728882297873497,
-0.00572733860462904,
0.0017683220794424415,
-0.04090775176882744,
0.004611222539097071,
0.07595591247081757,
0.016772912815213203,
0.05732237547636032,
-0.013339245691895485,
0.03898370638489723,
-0.006218708585947752,
-0.027136417105793953,
0.0033117160201072693,
-0.023464305326342583,
0.03633518144488335,
0.007792871445417404,
0.023401182144880295,
0.034254077821969986,
-0.03786300867795944,
-0.08514958620071411,
-0.016538221389055252,
-0.07162108272314072,
0.034957293421030045,
-0.08756934851408005,
0.01758504845201969,
0.047898005694150925,
-0.013479916378855705,
-0.019631171599030495,
-0.056119654327631,
-0.00017205101903527975,
-0.05290912836790085,
0.016139667481184006,
-0.0020905397832393646,
0.013398378156125546,
-0.01599479839205742,
0.3784297704696655,
-0.007644706405699253,
-0.050815220922231674,
-0.061746757477521896,
-0.00029748029191978276,
-0.018353234976530075,
-0.002826014067977667,
0.025180747732520103,
-0.05988287925720215,
0.04314080625772476,
0.04540028050541878,
0.030971664935350418,
-0.015188486315310001,
0.07776983082294464,
-0.007016556337475777,
-0.016336876899003983,
-0.015472029335796833,
0.02972816489636898,
-0.03477458655834198,
-0.06124915927648544,
0.07317762821912766,
-0.036136846989393234,
-0.008066433481872082,
0.050323665142059326,
-0.01548692025244236,
0.024884024634957314,
0.067986398935318,
0.07403704524040222,
0.061823002994060516,
-0.015074547380208969,
0.048003897070884705,
0.06629442423582077,
-0.025895459577441216,
-0.03933345526456833,
-0.05292323976755142,
0.013023854233324528,
0.02893172763288021,
0.07524334639310837,
-0.02361571602523327,
0.015605557709932327,
0.053278643637895584,
-0.021273525431752205,
-0.060339294373989105,
-0.011554781347513199,
-0.07398970425128937,
0.005850099492818117,
0.10768454521894455,
-0.005060598254203796,
0.03500131890177727,
0.027817731723189354,
0.028125733137130737,
0.017402540892362595,
0.09828190505504608,
-0.05342809855937958,
0.014725681394338608,
-0.02254670113325119,
-0.01641215942800045,
-0.01395711861550808,
0.023366276174783707,
0.02855074591934681,
0.009725778363645077,
-0.06789477914571762,
-0.038410384207963943,
-0.005438054446130991,
0.035506416112184525,
0.021611500531435013,
-0.018085073679685593,
-0.05987050384283066,
0.011499031446874142,
-0.03078051097691059,
0.016645213589072227,
0.012946286238729954,
0.01903589256107807,
0.07966750860214233,
-0.0014744210056960583,
-0.009995413944125175,
-0.005348401144146919,
-0.005403227172791958,
-0.029642343521118164,
0.036175474524497986,
-0.04360172897577286,
0.0022288698237389326,
0.05817214027047157,
0.01929972879588604,
0.06645714491605759,
-0.0324348621070385,
-0.040255334228277206,
-0.020719127729535103,
-0.020445775240659714,
0.016796229407191277,
0.008441141806542873,
-0.02600703202188015,
0.005209460388869047,
-0.05268736183643341,
-0.045400165021419525,
0.009447681717574596,
-0.023806370794773102,
-0.09891654551029205,
0.014622031711041927,
-0.038676146417856216,
-0.011114295572042465,
0.09613185375928879,
-0.008788425475358963,
0.05952488258481026,
0.0020274128764867783,
0.05027776584029198,
0.021896038204431534,
-0.018638569861650467,
-0.007506115827709436,
-0.016638321802020073,
-0.005724345333874226,
-0.002560562686994672,
0.030260369181632996,
0.03268465772271156,
-0.030645618215203285,
-0.050225213170051575,
0.11450615525245667,
-0.02427426539361477,
0.03054605983197689,
-0.0478794239461422,
0.009140504524111748,
-0.021985294297337532,
0.011960471048951149,
-0.03510075435042381,
-0.32057541608810425,
0.015969503670930862,
0.00926856230944395,
0.013380295597016811,
0.0566118061542511,
-0.009341904893517494,
-0.01200066413730383,
-0.00004753182292915881,
-0.038039520382881165,
-0.02157595567405224,
0.025618433952331543,
0.036801353096961975,
-0.0755622535943985,
-0.0766543447971344,
-0.007343740202486515,
0.05806295573711395,
-0.008581889793276787,
-0.051583994179964066,
0.009842503815889359,
-0.02087877318263054,
0.004004948772490025,
-0.04953021556138992,
-0.033538758754730225,
0.05043588578701019,
0.08361884951591492,
0.045598048716783524,
0.08883938938379288,
-0.050795476883649826,
-0.09042719751596451,
-0.04142196476459503,
0.02225809544324875,
-0.0005141135188750923,
0.027074359357357025,
-0.043102774769067764,
0.04307667538523674,
-0.02706664614379406,
0.03518792614340782,
-0.015429737977683544,
0.018829036504030228,
0.031171491369605064,
-0.035466354340314865,
0.026316381990909576,
-0.04225559160113335,
-0.04317914694547653,
-0.017047548666596413,
0.02172841690480709,
0.0010060048662126064,
0.06609724462032318,
0.0030520353466272354,
-0.008337740786373615,
0.027969349175691605,
0.02834312431514263,
0.01279524527490139,
0.02901589684188366,
0.026737362146377563,
-0.06534729897975922,
-0.07957287132740021,
0.0004112234164495021,
-0.09701849520206451,
-0.005137030966579914,
-0.01945405639708042,
-0.017392711713910103,
0.052352968603372574,
-0.06476014107465744,
0.00593734672293067,
-0.00659499317407608,
0.04103024676442146,
0.022369084879755974,
0.0715954378247261,
0.036665186285972595,
-0.010473676957190037,
0.06029986962676048,
-0.0724661722779274,
0.010952113196253777,
0.09456299990415573,
0.015246286988258362,
-0.043422721326351166,
-0.009920123964548111,
-0.09033746272325516,
0.06217821314930916,
0.006071291398257017,
-0.04404280334711075,
0.004582604393362999,
-0.007450618781149387,
-0.02059723436832428,
0.016480719670653343,
-0.029487261548638344,
0.003111693076789379,
-0.05393364652991295,
0.01623038575053215,
0.010844576172530651,
-0.032640401273965836,
-0.021721206605434418,
0.024430980905890465,
-0.01934952102601528,
-0.016019785776734352,
-0.2867562472820282,
0.04066557437181473,
0.0517493337392807,
-0.038602106273174286,
-0.03285328298807144,
0.009091575630009174,
-0.04897894710302353,
-0.019257549196481705,
-0.04940759763121605,
0.04945012181997299,
0.05569879710674286,
0.04251854866743088,
0.09445250034332275,
0.0576203390955925,
-0.009985668584704399,
0.013235719874501228,
0.08610621094703674,
-0.00460367277264595,
-0.0020580405835062265,
-0.07783664762973785,
0.009051203727722168,
-0.08135560899972916,
0.102166086435318,
0.01999998651444912,
-0.051174018532037735,
0.011998257599771023,
-0.020821573212742805,
0.0601770356297493,
-0.04454793781042099,
-0.006367907393723726,
0.011065014638006687,
0.001878255745396018,
0.03411240130662918,
0.03963342681527138,
0.029270492494106293,
0.009855931624770164,
-0.04614047706127167,
0.08536822348833084,
0.009348378516733646,
0.03224864602088928,
-0.03451598808169365,
0.010187946259975433,
-0.00025798616115935147,
0.02082842029631138,
0.012243908829987049,
-0.039902813732624054,
-0.04926192760467529,
-0.020051881670951843,
-0.021607503294944763,
-0.011662732809782028,
-0.00129037129227072,
-0.010755308903753757,
0.05115613713860512,
0.01756175048649311,
0.009500591084361076,
0.013353490270674229,
-0.0039690956473350525,
-0.034351058304309845,
-0.004433345515280962,
-0.01264934241771698,
-0.03269423916935921,
0.04818491265177727,
-0.037272922694683075,
0.04422712326049805,
0.04859701171517372
] |
PMID:18734
|
Cryoenzymology in mixed solvents without cosolvent effects on enzyme specific activity.
|
Water-soluble polyelectrolytes in interaction with proteins are described. These polyelectrolytes make it possible to investigate enzyme-catalyzed reactions in cooled mixed solvents without the usual effects of their organic solvent component on enzyme specific activity. The applicability of techniques developed is illustrated by results obtained on several systems. The possibility of an electrostatic "sorting out" of solvents and its potentialities in cryoenzymology are discussed.
|
Cryoenzymology in mixed solvents without cosolvent effects on enzyme specific activity. Water-soluble polyelectrolytes in interaction with proteins are described. These polyelectrolytes make it possible to investigate enzyme-catalyzed reactions in cooled mixed solvents without the usual effects of their organic solvent component on enzyme specific activity. The applicability of techniques developed is illustrated by results obtained on several systems. The possibility of an electrostatic "sorting out" of solvents and its potentialities in cryoenzymology are discussed.
|
[
-0.0754355788230896,
0.03603668883442879,
-0.03014044463634491,
0.017220327630639076,
0.00807674415409565,
-0.0503091961145401,
0.03366389498114586,
0.032084960490465164,
0.00975784007459879,
-0.02875879779458046,
0.011416980996727943,
-0.012088870629668236,
-0.009839292615652084,
0.02161099575459957,
0.003989150747656822,
0.021246179938316345,
0.01729206182062626,
0.020441189408302307,
-0.09955796599388123,
0.046897612512111664,
-0.020608602091670036,
-0.0022294283844530582,
0.03961467370390892,
-0.03595862165093422,
0.0034860214218497276,
0.06113461032509804,
0.02750558592379093,
0.04108267277479172,
-0.020622121170163155,
-0.20198966562747955,
-0.001654246123507619,
-0.03454424440860748,
-0.003050324274227023,
-0.01775718480348587,
0.04734894260764122,
0.03333776816725731,
-0.0020866836421191692,
-0.03429592400789261,
-0.06664595007896423,
-0.006361209321767092,
0.014822903089225292,
0.005934534594416618,
-0.03935381770133972,
0.013756844215095043,
0.021950604394078255,
-0.013453132472932339,
-0.016533438116312027,
0.01247025839984417,
0.038815490901470184,
-0.01847873628139496,
0.02275940030813217,
0.009365790523588657,
-0.04605630040168762,
-0.022686613723635674,
0.028212379664182663,
0.029253903776407242,
-0.01644640974700451,
0.005191469565033913,
-0.02016616053879261,
0.015346385538578033,
-0.006992227863520384,
0.014930367469787598,
-0.09573382139205933,
0.07441527396440506,
0.10732574015855789,
-0.004143824335187674,
-0.027669109404087067,
-0.027267128229141235,
0.05046520009636879,
0.047057632356882095,
-0.0643521174788475,
0.11308389157056808,
0.027261851355433464,
0.013203716836869717,
0.003139310050755739,
-0.0720180943608284,
-0.016977796331048012,
0.02478068880736828,
-0.03589029237627983,
0.010603980161249638,
0.01078031025826931,
-0.03647564724087715,
0.06633948534727097,
0.001751603209413588,
-0.04052525386214256,
0.06289706379175186,
-0.020706741139292717,
-0.06339628994464874,
0.048041339963674545,
0.029587730765342712,
0.006409074645489454,
-0.03228471055626869,
-0.020195575430989265,
0.0057962071150541306,
-0.04044806584715843,
0.08151241391897202,
0.05234552174806595,
0.012119504623115063,
0.07827156037092209,
0.43727123737335205,
-0.01582586020231247,
0.03443046286702156,
-0.023274894803762436,
-0.05675215274095535,
-0.07923132181167603,
-0.04567846655845642,
-0.04152781143784523,
-0.025332044810056686,
-0.01633300445973873,
0.017475314438343048,
0.04853082820773125,
-0.024766674265265465,
0.023983072489500046,
-0.08690128475427628,
-0.019025031477212906,
0.031643345952034,
-0.021324198693037033,
0.02265143021941185,
-0.022518793120980263,
0.004749759566038847,
-0.019120223820209503,
-0.006219266448169947,
0.039187584072351456,
-0.001832504291087389,
0.046099238097667694,
-0.004533790051937103,
-0.0381641648709774,
0.0701960101723671,
0.016945626586675644,
0.023047935217618942,
0.03658184036612511,
0.026391059160232544,
0.006483092438429594,
-0.024965913966298103,
0.025029366835951805,
0.01624692790210247,
0.028930874541401863,
-0.022035805508494377,
0.09970054775476456,
0.006504554767161608,
-0.017898522317409515,
-0.025831561535596848,
-0.03125821799039841,
-0.05744558200240135,
-0.023893529549241066,
0.03533695638179779,
0.007742017041891813,
0.035995133221149445,
-0.008342931047081947,
0.02415532059967518,
-0.030016161501407623,
0.07384377717971802,
-0.06480154395103455,
0.06310594826936722,
0.013133889995515347,
-0.049810100346803665,
0.0317266508936882,
-0.05045429617166519,
-0.006750135216861963,
0.01795990765094757,
-0.08217637240886688,
0.010706298984587193,
0.017164485529065132,
0.10691005736589432,
-0.025904688984155655,
-0.032305311411619186,
0.030282899737358093,
-0.0037397323176264763,
0.01870170794427395,
-0.04150023311376572,
-0.028775636106729507,
0.01045855414122343,
0.018053878098726273,
-0.002390503417700529,
-0.0022124270908534527,
0.033334679901599884,
0.002171432366594672,
0.025665434077382088,
0.011827567592263222,
0.013888325542211533,
-0.005399168003350496,
-0.016308173537254333,
-0.017043935135006905,
0.041634827852249146,
0.05728277936577797,
-0.024023843929171562,
-0.028056921437382698,
0.04598208889365196,
-0.02932734228670597,
0.045987583696842194,
-0.056088514626026154,
-0.031434569507837296,
-0.06393181532621384,
0.0527787059545517,
-0.02237617038190365,
0.00045495826634578407,
-0.00865256879478693,
0.002959876786917448,
0.025852784514427185,
-0.013741331174969673,
0.097046859562397,
0.03996735066175461,
-0.02221016399562359,
0.027819864451885223,
0.07222236692905426,
0.04654902219772339,
0.05523766949772835,
0.06074567511677742,
0.04440617561340332,
0.057675912976264954,
-0.03307032957673073,
-0.021394282579421997,
-0.05432365834712982,
-0.030725300312042236,
-0.044155362993478775,
0.022741183638572693,
0.023114103823900223,
0.019129374995827675,
0.01717277616262436,
-0.011211016215384007,
0.03181581199169159,
-0.08373744785785675,
-0.07110665738582611,
-0.31997185945510864,
-0.0621805377304554,
-0.06658732891082764,
-0.029852069914340973,
0.08312391489744186,
-0.036692824214696884,
0.02496947906911373,
-0.035299502313137054,
-0.007921296171844006,
-0.035935427993535995,
0.05398014187812805,
0.044719818979501724,
-0.03948403149843216,
-0.04394160956144333,
-0.014780431054532528,
-0.011431746184825897,
0.024888569489121437,
-0.0705062747001648,
0.004810589365661144,
0.05946777015924454,
0.006649558432400227,
-0.01959524303674698,
0.00877109169960022,
0.046696845442056656,
0.06570080667734146,
-0.05882821977138519,
0.12858016788959503,
0.019555015489459038,
-0.01743335649371147,
0.01758928969502449,
-0.028994593769311905,
0.018763860687613487,
-0.041793856769800186,
-0.09512115269899368,
-0.030539080500602722,
-0.04012911021709442,
-0.02522188425064087,
-0.04613426327705383,
0.021075677126646042,
0.03207745403051376,
-0.02178160846233368,
0.018444275483489037,
0.03678333759307861,
-0.009755222126841545,
-0.03793196380138397,
-0.02298927493393421,
0.04751497879624367,
-0.008665775880217552,
-0.03793710470199585,
0.023463847115635872,
0.04552602767944336,
-0.04586024209856987,
0.039402976632118225,
-0.050109609961509705,
-0.05788794904947281,
0.008553115651011467,
0.013371430337429047,
0.008993946947157383,
-0.02354814112186432,
0.005222187843173742,
0.04808303341269493,
0.007659483700990677,
-0.03953917697072029,
0.07128539681434631,
0.0034008740913122892,
0.0010174141498282552,
0.0223469790071249,
-0.05343443527817726,
0.06762760877609253,
-0.025445474311709404,
-0.007770819589495659,
0.08567182719707489,
-0.015469761565327644,
0.04631267488002777,
0.014830314554274082,
0.027078870683908463,
-0.01590326428413391,
-0.0628882423043251,
-0.03562968969345093,
0.02223173901438713,
-0.01635756902396679,
0.014857002533972263,
0.008630139753222466,
-0.02491002157330513,
0.007615501061081886,
0.044825613498687744,
-0.004759317729622126,
-0.008381051942706108,
-0.01495986245572567,
-0.019071554765105247,
0.027096059173345566,
-0.004823050927370787,
-0.037642743438482285,
0.00513495272025466,
0.021977553144097328,
0.039468299597501755,
-0.2659645080566406,
0.023823902010917664,
0.044594939798116684,
-0.014386022463440895,
-0.007036593742668629,
0.030254783108830452,
-0.012359149754047394,
-0.02126227505505085,
-0.026057586073875427,
0.0423157662153244,
-0.04137536883354187,
-0.013405794277787209,
0.04343709349632263,
0.03529391065239906,
-0.01763284020125866,
0.03251073509454727,
-0.016766931861639023,
-0.08134280145168304,
0.00455810222774744,
-0.02681073173880577,
0.03173162415623665,
-0.016205869615077972,
0.18141613900661469,
-0.0255022831261158,
-0.009920800104737282,
0.04972193390130997,
0.018370147794485092,
0.05662817135453224,
-0.04920322448015213,
-0.0031165757682174444,
-0.015299847349524498,
-0.00489061651751399,
0.03465196117758751,
-0.027665747329592705,
-0.0027664420194923878,
0.026577627286314964,
-0.010173722170293331,
0.02214920148253441,
-0.005409779492765665,
0.005620837677270174,
0.006378974765539169,
-0.025751059874892235,
-0.051868148148059845,
-0.06624222546815872,
-0.005423576105386019,
-0.013439522124826908,
-0.0013085053069517016,
-0.04496030509471893,
-0.020754706114530563,
0.02347131259739399,
-0.037353936582803726,
-0.04788382723927498,
0.023193178698420525,
0.004082105588167906,
-0.019348029047250748,
0.025189610198140144,
-0.04468042403459549,
-0.03454108536243439,
-0.01687755435705185,
0.0001396813604515046,
0.016725655645132065,
0.0181150920689106,
-0.04969580098986626,
0.08894538879394531,
-0.013188563287258148
] |
PMID:18735
|
A role for asparaginyl-tRNA in the regulation of asparagine synthetase in a mammalian cell line.
|
The expression of asparagine synthetase activity [L-aspartate:ammonia ligase (AMP-forming), EC 6.3.1.1] in cultured Chinese hamster ovary (CHO) cells is regulated by asparagine. After transfer of CHO cells from an asparagine-supplemented medium to a medium lacking asparagine, activity increases 1.5- to 2-fold. If asparagine is added back to the medium, activity returns to control levels. To test the possible involvement of Asn-tRNAAsn in regulating the levels of asparagine synthetase, we have examined the levels of asparagine synthetase in a mutant of CHO cells containing a temperature-sensitive asparaginyl-tRNA synthetase [L-asparagine:tRNA ligase (AMP-forming), EC 6.1.1.22]. Under conditions of limited asparaginyl-tRNA synthetase activity in the mutant, there is a 2- to 3-fold increase in the level of asparagine synthetase activity. Under identical conditions, there is no change in asparagine synthetase activity in the wild type. This correlation between asparaginyl-tRNA synthetase activity and asparagine synthetase levels may be a consequence of a direct role of tRNAAsn in the regulation of the in vivo expression of the asparagine synthetase structural gene.
|
A role for asparaginyl-tRNA in the regulation of asparagine synthetase in a mammalian cell line. The expression of asparagine synthetase activity [L-aspartate:ammonia ligase (AMP-forming), EC 6.3.1.1] in cultured Chinese hamster ovary (CHO) cells is regulated by asparagine. After transfer of CHO cells from an asparagine-supplemented medium to a medium lacking asparagine, activity increases 1.5- to 2-fold. If asparagine is added back to the medium, activity returns to control levels. To test the possible involvement of Asn-tRNAAsn in regulating the levels of asparagine synthetase, we have examined the levels of asparagine synthetase in a mutant of CHO cells containing a temperature-sensitive asparaginyl-tRNA synthetase [L-asparagine:tRNA ligase (AMP-forming), EC 6.1.1.22]. Under conditions of limited asparaginyl-tRNA synthetase activity in the mutant, there is a 2- to 3-fold increase in the level of asparagine synthetase activity. Under identical conditions, there is no change in asparagine synthetase activity in the wild type. This correlation between asparaginyl-tRNA synthetase activity and asparagine synthetase levels may be a consequence of a direct role of tRNAAsn in the regulation of the in vivo expression of the asparagine synthetase structural gene.
|
[
-0.03508743271231651,
-0.025363588705658913,
-0.02491532824933529,
0.005538129713386297,
-0.00842264760285616,
0.05722489207983017,
0.06147008761763573,
0.030184421688318253,
-0.061221230775117874,
0.031008344143629074,
-0.010000089183449745,
-0.0752803236246109,
0.04656421020627022,
0.06388619542121887,
-0.02405572682619095,
-0.04292914643883705,
-0.008126013912260532,
-0.053093139082193375,
-0.03730562701821327,
0.03431349992752075,
0.009584305807948112,
-0.008949213661253452,
0.08034448325634003,
-0.007428470067679882,
0.04206371679902077,
0.005138217471539974,
-0.0243229940533638,
-0.017130047082901,
-0.03957020863890648,
-0.1986767053604126,
0.021588914096355438,
-0.026780422776937485,
0.026485338807106018,
-0.03349003940820694,
-0.027033589780330658,
0.02257608063519001,
-0.005024039186537266,
-0.02850465476512909,
-0.002732182154431939,
0.04369042068719864,
0.027514727786183357,
-0.0239701010286808,
0.019783489406108856,
-0.05149079114198685,
0.06448694318532944,
0.010314625687897205,
-0.029349641874432564,
-0.027772165834903717,
0.05058979243040085,
-0.026629170402884483,
0.01691100373864174,
-0.04304049164056778,
0.01838236302137375,
0.018290361389517784,
0.015521101653575897,
-0.005228455178439617,
0.051973603665828705,
0.11060415953397751,
0.02663535811007023,
-0.0010103014064952731,
0.04950486868619919,
-0.004199787974357605,
-0.11025679111480713,
0.03852666914463043,
0.04625588282942772,
0.03122200258076191,
0.016010012477636337,
-0.056319862604141235,
0.02692412957549095,
0.05665208399295807,
-0.0750962644815445,
-0.013089869171380997,
-0.020491451025009155,
0.0622752420604229,
0.028675014153122902,
-0.01726200431585312,
-0.006328059360384941,
0.021088792011141777,
0.04588579386472702,
-0.007292483933269978,
0.005221444647759199,
-0.031962715089321136,
0.03875158354640007,
-0.000346784305293113,
-0.03738753870129585,
0.01605771854519844,
0.02043003961443901,
-0.03745177388191223,
-0.04482245072722435,
-0.018805120140314102,
-0.06666114181280136,
-0.032014187425374985,
0.012800293043255806,
-0.029340621083974838,
-0.06671909987926483,
0.03927525505423546,
0.033562544733285904,
-0.03281012549996376,
-0.007586073130369186,
0.3744935095310211,
-0.04289969056844711,
-0.03020329959690571,
-0.12898245453834534,
0.009807479567825794,
0.02708515152335167,
-0.05241835489869118,
0.007864042185246944,
0.035106103867292404,
0.0019913320429623127,
0.0664042979478836,
0.07724568247795105,
0.01965472847223282,
-0.05107806250452995,
0.009593307040631771,
-0.03993860259652138,
0.030011598020792007,
0.015055338852107525,
0.011764359660446644,
-0.06818241626024246,
0.03754769265651703,
-0.030473880469799042,
-0.003157024271786213,
0.003846278879791498,
-0.00928801391273737,
0.04504287242889404,
0.022033942863345146,
-0.052714429795742035,
0.08800844848155975,
0.02295256219804287,
0.027948204427957535,
0.08507169783115387,
0.06437880545854568,
0.016970667988061905,
0.027137089520692825,
-0.005616781301796436,
-0.08651626855134964,
-0.02226608619093895,
-0.05339853838086128,
-0.01643322966992855,
-0.02156589739024639,
-0.0034322470892220736,
-0.07142461091279984,
-0.006579569540917873,
-0.055198244750499725,
-0.016662035137414932,
0.10609032213687897,
-0.019600141793489456,
0.05197566747665405,
-0.002256130101159215,
-0.00900074653327465,
0.004838361404836178,
0.03006644733250141,
0.018314303830266,
0.011497050523757935,
-0.007991332560777664,
0.013341405428946018,
-0.0071640522219240665,
-0.028338966891169548,
-0.021678917109966278,
-0.003081429284065962,
-0.06232480704784393,
-0.01704355888068676,
-0.04003620520234108,
0.012110158801078796,
0.06314080208539963,
-0.007394059561192989,
-0.05204242467880249,
0.04452620446681976,
-0.023770291358232498,
-0.0043108719401061535,
0.009465685114264488,
-0.018092289566993713,
-0.07464846968650818,
0.009712250903248787,
-0.01865890622138977,
0.013898052275180817,
-0.01189566869288683,
-0.024773769080638885,
-0.01686922460794449,
0.05197137966752052,
0.02905471809208393,
-0.06206339970231056,
0.023025473579764366,
-0.0059561049565672874,
-0.016138730570673943,
-0.011294793337583542,
-0.026167649775743484,
-0.017663979902863503,
0.02060183510184288,
0.05296175926923752,
-0.053865667432546616,
0.014280988834798336,
-0.0731041207909584,
0.021026184782385826,
-0.0040260255336761475,
-0.053360674530267715,
0.030929291620850563,
0.05433516204357147,
-0.028232980519533157,
-0.0058725676499307156,
0.05425562709569931,
0.0547369010746479,
0.019343530759215355,
0.042378827929496765,
-0.04141717031598091,
-0.02308288775384426,
0.005242547485977411,
-0.0055314344353973866,
0.026731813326478004,
0.014936145395040512,
-0.09423726052045822,
0.02877325564622879,
-0.038122307509183884,
0.026664888486266136,
-0.06774615496397018,
0.0707450807094574,
-0.03375958651304245,
-0.012333991937339306,
0.06705097109079361,
0.007347565609961748,
0.02132636122405529,
-0.06435000896453857,
0.011992228217422962,
-0.34756162762641907,
-0.0057123941369354725,
0.035731565207242966,
-0.02290959469974041,
0.01762418821454048,
0.007153558544814587,
-0.05310875177383423,
0.022214548662304878,
0.034460533410310745,
-0.011514618992805481,
0.025628915056586266,
0.10746406763792038,
0.004917226731777191,
0.0015577751910313964,
-0.015673674643039703,
0.05837190896272659,
-0.004029651638120413,
-0.004728455096483231,
0.022229863330721855,
0.01278318278491497,
-0.010962090454995632,
-0.00020636277622543275,
-0.008211534470319748,
-0.01007949560880661,
0.02138378843665123,
-0.016981786116957664,
0.07739367336034775,
-0.004416061565279961,
0.025872964411973953,
-0.0015238203341141343,
0.03639768436551094,
0.04290223494172096,
-0.009264454245567322,
-0.1329648196697235,
0.012319975532591343,
0.02113640122115612,
0.002960704965516925,
0.006949994247406721,
-0.03540368005633354,
0.029814843088388443,
0.008386333473026752,
-0.06450027972459793,
-0.03512132540345192,
-0.045517902821302414,
-0.056550800800323486,
-0.02171354368329048,
0.015487311407923698,
0.006374715361744165,
0.06442438811063766,
-0.056207749992609024,
0.021265976130962372,
-0.039576753973960876,
0.009826773777604103,
-0.011387807317078114,
-0.0493316613137722,
0.0005185328191146255,
0.023979542776942253,
-0.02806481346487999,
-0.019256113097071648,
0.031896647065877914,
0.022700050845742226,
0.025236254557967186,
-0.0023632615339010954,
0.024026216939091682,
-0.04314252361655235,
-0.018579911440610886,
-0.049612365663051605,
0.005976015701889992,
0.03872473165392876,
0.02399790659546852,
0.004622124135494232,
0.043996457010507584,
-0.029656538739800453,
0.06194421276450157,
0.008558090776205063,
0.007584199775010347,
-0.0792340338230133,
-0.057821158319711685,
-0.1001386046409607,
0.03218216076493263,
-0.04345259815454483,
-0.05376063659787178,
0.064607173204422,
-0.058751899749040604,
-0.017640022560954094,
0.029571281746029854,
0.015425394289195538,
0.034824907779693604,
0.1002972200512886,
-0.011510120704770088,
-0.034109972417354584,
-0.03164856508374214,
-0.000015346280633821152,
-0.04974692687392235,
0.06406400352716446,
0.004088457673788071,
-0.2337428778409958,
-0.002306864596903324,
0.012222006916999817,
0.004268244840204716,
-0.02439475990831852,
0.06365818530321121,
0.05622660368680954,
-0.03546768054366112,
-0.018623681738972664,
0.05249255895614624,
0.06761297583580017,
-0.004142912104725838,
0.05328560248017311,
0.095587819814682,
0.06417819857597351,
0.014366330578923225,
0.06519223749637604,
-0.0015782439149916172,
0.05073640123009682,
-0.05062065273523331,
-0.01971599832177162,
-0.01826239563524723,
0.13817556202411652,
0.003237506840378046,
0.039187442511320114,
-0.009089837782084942,
-0.03947232663631439,
0.03690683841705322,
0.011098101735115051,
-0.009433525614440441,
-0.021450752392411232,
0.020455913618206978,
0.1002562940120697,
0.03350033238530159,
0.060151103883981705,
0.02126668021082878,
0.012857026420533657,
0.08510949462652206,
-0.0232219435274601,
-0.02020108699798584,
0.02256481721997261,
0.029102101922035217,
-0.07254467904567719,
-0.03154679015278816,
0.03495430201292038,
-0.07768561691045761,
-0.04314076527953148,
-0.07778497040271759,
0.011323214508593082,
0.01641690917313099,
-0.02203771471977234,
-0.012857919558882713,
0.04554004967212677,
0.03006371669471264,
0.01142064854502678,
-0.0404740646481514,
-0.03501785919070244,
-0.039832089096307755,
0.015972565859556198,
0.026975033804774284,
-0.013144733384251595,
-0.02595866471529007,
0.0018994875717908144,
0.11141551285982132,
-0.014299609698355198
] |
PMID:18736
|
Isolation of a chloroplast N,N'-dicyclohexylcarbodiimide-binding proteolipid, active in proton translocation.
|
The N,N'-dicyclohexylcarbodiimide-binding proteolipid from lettuce chloroplast membranes has been purified by a novel, rapid technique involving I-butanol extraction and ether precipitation. Reconstitution of this proteolipid into liposomes composed of chloroplast lipids and subsequent incorporation of bacteriorhodopsin resulted in the formation of liposomes exhibiting a light-dependent accumulation of protons. This accumulation was significantly enhanced upon addition of N,N'-dicyclohexylcarbodiimide at concentrations similar to those that inhibit chloroplast adenosinetriphosphatase activity. Radioactively labeled N,N'-dicyclohexylcarbodiimide was found to be incorporated essentially into the proteolipid of the reconstituted liposomes. These results suggest that the functional unit responsible for proton channeling in the chloroplast membrane has been isolated and reconstituted in the native state.
|
Isolation of a chloroplast N,N'-dicyclohexylcarbodiimide-binding proteolipid, active in proton translocation. The N,N'-dicyclohexylcarbodiimide-binding proteolipid from lettuce chloroplast membranes has been purified by a novel, rapid technique involving I-butanol extraction and ether precipitation. Reconstitution of this proteolipid into liposomes composed of chloroplast lipids and subsequent incorporation of bacteriorhodopsin resulted in the formation of liposomes exhibiting a light-dependent accumulation of protons. This accumulation was significantly enhanced upon addition of N,N'-dicyclohexylcarbodiimide at concentrations similar to those that inhibit chloroplast adenosinetriphosphatase activity. Radioactively labeled N,N'-dicyclohexylcarbodiimide was found to be incorporated essentially into the proteolipid of the reconstituted liposomes. These results suggest that the functional unit responsible for proton channeling in the chloroplast membrane has been isolated and reconstituted in the native state.
|
[
-0.06655499339103699,
-0.04970913007855415,
-0.023611700162291527,
-0.005887608043849468,
0.024935219436883926,
-0.06372954696416855,
0.043103646486997604,
0.015935059636831284,
-0.029625345021486282,
0.02913929894566536,
0.03997591510415077,
-0.08830615133047104,
-0.001832254114560783,
0.02386303059756756,
0.012403090484440327,
-0.03444375470280647,
0.011419052258133888,
0.008058629930019379,
-0.061992987990379333,
0.013419042341411114,
0.012068033218383789,
-0.04558770731091499,
-0.040282756090164185,
-0.03260895982384682,
0.026411809027194977,
0.05373319983482361,
0.004708303604274988,
0.002641762839630246,
-0.062748022377491,
-0.18194277584552765,
0.03537622466683388,
-0.008945579640567303,
-0.021261081099510193,
-0.009896915405988693,
-0.0037376806139945984,
0.0070879217237234116,
0.001005789847113192,
-0.044540490955114365,
-0.041609469801187515,
0.04642380401492119,
0.04947035759687424,
-0.007075590081512928,
-0.01179291307926178,
-0.004870581440627575,
0.029463928192853928,
-0.012753033079206944,
-0.01668025180697441,
0.0180461835116148,
0.06388616561889648,
-0.022877076640725136,
0.04087347909808159,
0.03147395700216293,
-0.004923894070088863,
0.0034042715560644865,
-0.01820535957813263,
-0.010965762659907341,
-0.01906154863536358,
0.04177930951118469,
0.020263681188225746,
0.01393789704889059,
0.012691231444478035,
0.04811357706785202,
-0.14326976239681244,
0.055286046117544174,
0.022036954760551453,
-0.015028263442218304,
-0.002742208307608962,
-0.051326874643564224,
0.047078683972358704,
0.08592474460601807,
-0.03017585724592209,
-0.018205251544713974,
-0.06448136270046234,
0.005684272851794958,
-0.023745840415358543,
0.006953145377337933,
-0.00474927993491292,
-0.010751121677458286,
0.046003758907318115,
-0.009584964253008366,
-0.04177025705575943,
-0.03729350119829178,
-0.043798740953207016,
0.03213635832071304,
-0.02120787464082241,
0.043808065354824066,
-0.03152093291282654,
-0.01776203140616417,
-0.008464747108519077,
0.0391438789665699,
-0.02564074844121933,
-0.10295918583869934,
0.0007862140773795545,
-0.016389532014727592,
-0.12505507469177246,
0.05051443353295326,
0.021318944171071053,
-0.06608269363641739,
0.03327501192688942,
0.37453821301460266,
-0.03872613608837128,
0.020884869620203972,
-0.00715008657425642,
-0.019171899184584618,
-0.0250595360994339,
0.04290478676557541,
0.021696293726563454,
-0.03190084174275398,
-0.018229831010103226,
0.012925544753670692,
0.05622391775250435,
0.0011676672147586942,
0.04382319375872612,
-0.035767801105976105,
-0.00033323062234558165,
-0.03850163146853447,
-0.004125431180000305,
-0.04415680468082428,
0.009695862419903278,
0.05571780726313591,
-0.04368121922016144,
0.02103809081017971,
0.05159340053796768,
0.04318777844309807,
0.057573698461055756,
0.01904289796948433,
-0.0236996877938509,
0.0783558189868927,
0.05213167518377304,
0.002645073691383004,
0.05487735569477081,
0.055402662605047226,
-0.0038237646222114563,
0.05704646557569504,
0.006860572844743729,
-0.006166127044707537,
0.08386638760566711,
-0.10463583469390869,
-0.012279505841434002,
-0.07118277251720428,
0.0200891625136137,
-0.02837098203599453,
-0.029841436073184013,
-0.06124655157327652,
-0.05602753162384033,
0.03613835200667381,
0.012193530797958374,
0.06826215237379074,
-0.04173390194773674,
0.05528216436505318,
-0.01380853820592165,
0.047080427408218384,
0.03989732638001442,
0.0402965173125267,
0.01359882578253746,
0.01590700075030327,
0.016042590141296387,
0.02291220985352993,
0.016453813761472702,
-0.025590527802705765,
-0.08871209621429443,
-0.020397424697875977,
0.018452323973178864,
0.10584228485822678,
0.026428328827023506,
-0.030244087800383568,
0.0027278440538793802,
-0.001400905312038958,
0.02023838274180889,
0.014352453872561455,
-0.037341248244047165,
0.0060515012592077255,
0.04640067741274834,
0.009764699265360832,
-0.016926243901252747,
0.023548072203993797,
-0.014957617036998272,
-0.009041435085237026,
0.007627126760780811,
-0.034435421228408813,
-0.015943460166454315,
-0.03192629665136337,
-0.041351377964019775,
-0.04077474772930145,
0.07965191453695297,
0.0031149350106716156,
-0.07675427943468094,
0.04367867484688759,
-0.0472765676677227,
0.04942942038178444,
-0.07798805832862854,
0.02086881920695305,
-0.04741879552602768,
0.02954268269240856,
-0.11331673711538315,
-0.09508568048477173,
-0.004436347633600235,
-0.02728315070271492,
0.018739543855190277,
-0.048542968928813934,
0.07998299598693848,
0.0022227726876735687,
-0.030215062201023102,
0.03585682436823845,
-0.01018690038472414,
-0.014774980954825878,
0.026831848546862602,
0.04153306409716606,
0.060458965599536896,
0.006608839612454176,
-0.021707991138100624,
-0.006615745835006237,
-0.08009395003318787,
-0.01791159249842167,
-0.07305845618247986,
0.016110900789499283,
0.032305698841810226,
0.039839841425418854,
0.0841042548418045,
-0.013192742131650448,
0.003425417933613062,
-0.05760328844189644,
-0.06499746441841125,
-0.30216526985168457,
-0.05091319605708122,
0.023003902286291122,
-0.0009920781012624502,
0.05937007814645767,
-0.01797935925424099,
0.07776452600955963,
0.02241305448114872,
0.02107614278793335,
-0.09830163419246674,
0.025519156828522682,
0.011385799385607243,
-0.06927213817834854,
-0.052038032561540604,
0.01270065177232027,
-0.01733265072107315,
0.0275796577334404,
-0.03462820127606392,
0.03685593232512474,
-0.002901989733800292,
0.044185761362314224,
-0.005244743544608355,
-0.034500375390052795,
0.031793903559446335,
0.06065038591623306,
-0.029079381376504898,
0.1300351619720459,
0.044616732746362686,
-0.0015893534291535616,
0.0018186286324635148,
0.0066048502922058105,
0.04798422008752823,
0.03874370828270912,
-0.09593212604522705,
-0.05840453878045082,
-0.03666389733552933,
0.005732819903641939,
-0.013861579820513725,
0.05646394565701485,
-0.004637158941477537,
0.03812801092863083,
-0.004238166380673647,
-0.032145824283361435,
-0.045225705951452255,
-0.011993437074124813,
-0.045368023216724396,
0.034022409468889236,
0.04769721254706383,
0.0534338504076004,
0.012564359232783318,
0.06349443644285202,
0.043298255652189255,
-0.0017288450617343187,
-0.011234031990170479,
0.019682928919792175,
0.008149068802595139,
0.042407259345054626,
0.02147224172949791,
-0.034101586788892746,
-0.018133660778403282,
-0.028815707191824913,
0.014191088266670704,
-0.011096645146608353,
0.044349733740091324,
0.050192590802907944,
-0.015183430165052414,
0.019762424752116203,
0.01424489077180624,
0.021258017048239708,
-0.02669942006468773,
0.01911829598248005,
0.05993679165840149,
-0.031563617289066315,
0.0020159301348030567,
0.03883717581629753,
0.051150206476449966,
-0.04298189654946327,
-0.04950518161058426,
-0.0987340658903122,
-0.022616727277636528,
0.04032988101243973,
-0.0025034791324287653,
0.05656236410140991,
0.007286238018423319,
-0.006661297753453255,
0.014327778480947018,
-0.028306012973189354,
-0.020500434562563896,
-0.003941428381949663,
-0.001957276137545705,
0.006924929562956095,
-0.013240385800600052,
-0.07148350775241852,
-0.04178912937641144,
0.045837465673685074,
0.020237620919942856,
-0.2619558274745941,
0.0457933284342289,
0.04798402264714241,
-0.02376065030694008,
-0.036308158189058304,
0.08189268410205841,
0.09567790478467941,
-0.003692101454362273,
-0.018818458542227745,
0.032496169209480286,
-0.013510978780686855,
-0.027423659339547157,
0.011571267619729042,
0.04152912646532059,
-0.01148876454681158,
0.06314826011657715,
0.029512351378798485,
-0.08246518671512604,
0.027145730331540108,
-0.010008228942751884,
-0.01726207695901394,
0.016707636415958405,
0.15825624763965607,
0.023761088028550148,
-0.037457093596458435,
0.039722539484500885,
-0.019609253853559494,
0.016938086599111557,
0.049233075231313705,
0.031237561255693436,
-0.002906435402110219,
0.028388988226652145,
0.05638778582215309,
-0.03057982586324215,
-0.029160043224692345,
-0.0012658832129091024,
0.04395558685064316,
-0.00763568002730608,
-0.0661759078502655,
-0.005915861576795578,
0.01658979058265686,
0.0010932362638413906,
-0.027042923495173454,
0.005385959055274725,
0.04763549938797951,
-0.07328583300113678,
-0.020296473056077957,
-0.045586735010147095,
0.015163428150117397,
-0.0013803895562887192,
-0.017321255058050156,
0.007964405231177807,
0.07070128619670868,
0.02553953416645527,
0.03265620023012161,
-0.023745466023683548,
-0.016338765621185303,
-0.0183492973446846,
-0.03075314126908779,
0.036007028073072433,
0.027494080364704132,
-0.055912334471940994,
-0.02203354239463806,
0.025376344099640846,
0.08419100195169449
] |
PMID:18744
|
Lack of effect of chronically administered thyrotropin-releasing hormone (TRH) on regional rat brain tyrosine hydroxylase activity.
|
Chronic treatment of adult male rats with TRH (1 or 10 mg/kg IP) for 5 or 9 days failed to alter the activity of tyrosine hydroxylase (TH), the enzyme regulating the rate-limiting step in catecholamine biosynthesis. In contrast, as previously described, chronic reserpine administration (0.5 mg/kg IP: 9 days) resulted in a significant rise in TH activity in midbrain, hypothalamus, pons-medulla and forebrain. These results suggest that the enhanced brain norepinephrine turnover reported to occur after treatment with TRH is not due to synthesis of new TH enzyme protein.
|
Lack of effect of chronically administered thyrotropin-releasing hormone (TRH) on regional rat brain tyrosine hydroxylase activity. Chronic treatment of adult male rats with TRH (1 or 10 mg/kg IP) for 5 or 9 days failed to alter the activity of tyrosine hydroxylase (TH), the enzyme regulating the rate-limiting step in catecholamine biosynthesis. In contrast, as previously described, chronic reserpine administration (0.5 mg/kg IP: 9 days) resulted in a significant rise in TH activity in midbrain, hypothalamus, pons-medulla and forebrain. These results suggest that the enhanced brain norepinephrine turnover reported to occur after treatment with TRH is not due to synthesis of new TH enzyme protein.
|
[
-0.01728779263794422,
0.03344004601240158,
-0.040678780525922775,
0.06476420164108276,
-0.018034527078270912,
0.0096990205347538,
0.045127298682928085,
0.007708467543125153,
-0.028407610952854156,
-0.038834359496831894,
-0.014834019355475903,
-0.043071866035461426,
0.010091371834278107,
0.09285490214824677,
-0.018245160579681396,
0.017467733472585678,
0.03689398616552353,
-0.01257390808314085,
-0.0342596173286438,
-0.014166554436087608,
-0.044452004134655,
0.01502374466508627,
0.06091172993183136,
-0.01906324177980423,
0.031830061227083206,
0.020033178851008415,
-0.08077238500118256,
0.005366449244320393,
-0.016094470396637917,
-0.16946092247962952,
0.05247778818011284,
-0.025331201031804085,
-0.0021017249673604965,
-0.01644488237798214,
0.010794954374432564,
0.02866174280643463,
-0.026180051267147064,
-0.005479767918586731,
0.0075990273617208,
0.02200327254831791,
0.04676680639386177,
0.03480423614382744,
0.002244005212560296,
-0.015557361766695976,
-0.00643567880615592,
0.03129492700099945,
-0.05301932245492935,
-0.03751483932137489,
0.06683781743049622,
-0.0007075842004269361,
0.013807931914925575,
0.024477755650877953,
0.02930200658738613,
0.08606850355863571,
0.042797867208719254,
0.02258407697081566,
0.024371737614274025,
0.04917275533080101,
-0.0023282889742404222,
0.06152411922812462,
0.023678146302700043,
0.04977917671203613,
-0.15370213985443115,
0.03639524057507515,
0.03308137133717537,
0.03132983297109604,
-0.016640832647681236,
0.018690939992666245,
0.04802802577614784,
0.008444491773843765,
-0.05049794539809227,
0.04009827971458435,
0.014416562393307686,
-0.005705024115741253,
0.0016412240220233798,
-0.0355800986289978,
-0.04245397076010704,
-0.03759419545531273,
0.032373372465372086,
0.00536308391019702,
0.056651704013347626,
-0.005824259947985411,
0.04613438993692398,
-0.03411462903022766,
0.0012153073912486434,
-0.003127825679257512,
-0.004606784321367741,
-0.02628290094435215,
0.014860251918435097,
0.004026002716273069,
0.00010057086910819635,
-0.05115310847759247,
0.04424987733364105,
0.007656949106603861,
-0.052513133734464645,
0.02937328815460205,
0.05761292204260826,
-0.029025455936789513,
-0.0796341747045517,
0.34329336881637573,
0.011188849806785583,
0.019555242732167244,
-0.1036069467663765,
-0.022422224283218384,
-0.024110019207000732,
0.033574339002370834,
0.0015677639748901129,
-0.0028854021802544594,
-0.04665994271636009,
0.09206333756446838,
0.04855016618967056,
0.0036943296436220407,
0.033054135739803314,
-0.0739784762263298,
-0.04354344680905342,
-0.025335827842354774,
0.021791065111756325,
0.02099059708416462,
0.01089978776872158,
0.0323181189596653,
-0.032209645956754684,
0.06249105930328369,
0.015706608071923256,
-0.04406759887933731,
0.012819832190871239,
-0.001063259318470955,
0.024252014234662056,
0.1122252568602562,
0.06114742532372475,
-0.0031170942820608616,
0.0502198301255703,
0.015223695896565914,
-0.02449408359825611,
0.02750031091272831,
0.054989323019981384,
0.030693266540765762,
0.04507913440465927,
-0.06838062405586243,
-0.010992666706442833,
0.009286980144679546,
0.005259476602077484,
-0.03643002733588219,
0.011208812706172466,
-0.06393696367740631,
-0.07869792729616165,
0.1319819688796997,
0.035377345979213715,
-0.04542015492916107,
-0.0225518885999918,
-0.01502225361764431,
0.013857005164027214,
0.06912089139223099,
-0.041701048612594604,
0.05742127075791359,
-0.015931064262986183,
0.051693469285964966,
-0.017360704019665718,
0.011957376264035702,
-0.036184508353471756,
-0.017915770411491394,
-0.04471120983362198,
0.005146893206983805,
-0.03001268394291401,
0.026665760204195976,
0.040946897119283676,
0.007490863557904959,
-0.04583695903420448,
-0.008686499670147896,
0.029904207214713097,
-0.000909580325242132,
-0.015171414241194725,
0.026456264778971672,
0.019889775663614273,
-0.005046826787292957,
-0.022359931841492653,
-0.04655667766928673,
0.03351341560482979,
-0.01063869521021843,
0.04748688265681267,
-0.02063979208469391,
0.02812177501618862,
-0.07354514300823212,
-0.04771491140127182,
0.022764481604099274,
0.01312336977571249,
0.02405981346964836,
-0.0010477928444743156,
0.03463249281048775,
0.018091561272740364,
0.019717304036021233,
-0.023632552474737167,
-0.013172520324587822,
-0.05596137419342995,
-0.09241869300603867,
-0.03942299261689186,
-0.0836305096745491,
-0.050064899027347565,
0.06831991672515869,
0.020119572058320045,
0.08875539153814316,
0.05873965471982956,
0.004945971537381411,
0.004972991533577442,
0.0429733470082283,
-0.0034564975649118423,
0.05443597584962845,
-0.05313637852668762,
0.006441549863666296,
0.07993774861097336,
0.0019755482207983732,
-0.02196090668439865,
0.021863581612706184,
0.011479214765131474,
0.009734872728586197,
-0.04044363275170326,
0.057988397777080536,
0.027864400297403336,
-0.021612700074911118,
0.032567523419857025,
0.021837133914232254,
0.1041097640991211,
-0.07054510712623596,
0.005508475936949253,
-0.2949373722076416,
-0.056944165378808975,
0.005890460219234228,
-0.04501213878393173,
0.04355962947010994,
-0.13116563856601715,
-0.04055250436067581,
-0.010381395928561687,
0.04063700884580612,
-0.023653706535696983,
0.007158174179494381,
0.09800862520933151,
-0.010565079748630524,
-0.03811606764793396,
0.029385188594460487,
0.03771942853927612,
0.057795312255620956,
-0.0584959052503109,
0.021117446944117546,
-0.014601959846913815,
0.024609940126538277,
-0.012493591755628586,
-0.02646125853061676,
-0.04339296370744705,
0.012927242554724216,
-0.018042344599962234,
0.07742466032505035,
-0.00020170050265733153,
0.013678489252924919,
-0.032923758029937744,
0.03372829034924507,
0.04536251351237297,
-0.027457870543003082,
-0.12671667337417603,
0.022404812276363373,
-0.0680222436785698,
-0.022699682042002678,
0.0010982286185026169,
-0.0020799231715500355,
-0.03761763498187065,
-0.09257511049509048,
-0.00032016169279813766,
-0.030118782073259354,
-0.0533655509352684,
-0.09217089414596558,
0.007565952837467194,
0.05062832683324814,
-0.00006267050048336387,
-0.01255058217793703,
-0.03510947898030281,
0.038962189108133316,
-0.012863817624747753,
-0.008041134104132652,
-0.01741216704249382,
0.012062675319612026,
-0.05000879615545273,
-0.0924324244260788,
-0.016921846196055412,
-0.015351276844739914,
-0.02283153124153614,
-0.007894621230661869,
0.038987431675195694,
-0.003973295912146568,
0.01642344519495964,
0.005786420311778784,
0.005959143862128258,
-0.005427701398730278,
0.0034679784439504147,
0.04763082414865494,
0.045303307473659515,
-0.004087108187377453,
0.05811096355319023,
-0.009071690030395985,
0.058826744556427,
0.017343470826745033,
-0.09206542372703552,
-0.0730460062623024,
-0.054530542343854904,
-0.05177942290902138,
0.04263010248541832,
-0.06138945743441582,
-0.07868867367506027,
0.07417549192905426,
0.02246147394180298,
-0.03528565540909767,
-0.02718544565141201,
0.04239645600318909,
-0.04098397493362427,
0.06083393841981888,
-0.003120343666523695,
-0.018628017976880074,
0.028867462649941444,
0.014306512661278248,
-0.0021878064144402742,
0.04065651819109917,
0.036330897361040115,
-0.2712370753288269,
-0.05862651765346527,
0.020652923732995987,
-0.06103194132447243,
-0.0021821833215653896,
0.07290291786193848,
-0.01918106898665428,
0.016622669994831085,
-0.05788137763738632,
0.030852947384119034,
-0.02346212975680828,
0.029806219041347504,
0.05811184272170067,
0.0693497285246849,
-0.014383124187588692,
-0.08412496745586395,
0.02615243010222912,
0.009218272753059864,
-0.007281752768903971,
-0.043783098459243774,
0.0076949880458414555,
-0.01259465329349041,
0.11566154658794403,
0.009629197418689728,
0.0004851589328609407,
0.004895130172371864,
0.050775740295648575,
0.05222661793231964,
-0.0003907771606463939,
0.020503193140029907,
-0.0756116509437561,
0.0029383504297584295,
0.04500717297196388,
0.005361237563192844,
0.04114965721964836,
0.036735329777002335,
-0.07988257706165314,
0.027178160846233368,
0.008647999726235867,
0.0329788513481617,
-0.03690921887755394,
0.04241739585995674,
-0.029118986800312996,
-0.01959598809480667,
0.08505343645811081,
-0.08717606216669083,
-0.02241317741572857,
-0.03330545499920845,
-0.030510298907756805,
0.0326244942843914,
-0.0018650088459253311,
-0.04216622933745384,
-0.012367052026093006,
0.03382999822497368,
0.026979463174939156,
0.03183870390057564,
-0.0051614660769701,
-0.013839404098689556,
0.003995303995907307,
0.02047613449394703,
-0.043319910764694214,
-0.03907264769077301,
-0.05553309619426727,
0.09813398122787476,
0.04356323555111885
] |
PMID:18746
|
Phosphohistone and glycogen synthase D phosphatase activities in a liver glycogen pellet preparation.
|
A liver glycogen pellet preparation previously found to contain synthase D phosphatase activity was shown to contain also phosphohistone phosphatase activity. Pellet phosphohistone phosphatase and synthase D phosphatase competed for the same substrates and appeared to be the same enzyme. ATP, a potent inhibitor, and G-6-P, a potent activator of the synthase phosphatase reaction, had little effect on the phosphohistone phosphatase reaction. These observations suggest that the ATP and G-6-P effects are relatively specific and are probably caused by binding to the synthase D substrate. The observed effects of NaCl and KCl were more complex. They stimulated phosphohistone phosphatase activity but strikingly inhibited synthase phosphatase activity. Sodium fluoride inhibited both reactions.
|
Phosphohistone and glycogen synthase D phosphatase activities in a liver glycogen pellet preparation. A liver glycogen pellet preparation previously found to contain synthase D phosphatase activity was shown to contain also phosphohistone phosphatase activity. Pellet phosphohistone phosphatase and synthase D phosphatase competed for the same substrates and appeared to be the same enzyme. ATP, a potent inhibitor, and G-6-P, a potent activator of the synthase phosphatase reaction, had little effect on the phosphohistone phosphatase reaction. These observations suggest that the ATP and G-6-P effects are relatively specific and are probably caused by binding to the synthase D substrate. The observed effects of NaCl and KCl were more complex. They stimulated phosphohistone phosphatase activity but strikingly inhibited synthase phosphatase activity. Sodium fluoride inhibited both reactions.
|
[
-0.033812541514635086,
0.003717840416356921,
0.05741877853870392,
-0.02921331487596035,
0.011888059787452221,
-0.03127588331699371,
0.056141939014196396,
-0.006858715321868658,
-0.08087863773107529,
-0.06073229759931564,
0.02263149432837963,
-0.04659734293818474,
-0.028399629518389702,
-0.012093259021639824,
-0.03709840774536133,
-0.03284076601266861,
-0.037871845066547394,
-0.0006167172105051577,
-0.06193632632493973,
0.021747741848230362,
-0.008488014340400696,
0.0602283701300621,
0.015902502462267876,
-0.03761816769838333,
0.039401981979608536,
0.016218753531575203,
0.06106584146618843,
0.019612601026892662,
-0.01460022758692503,
-0.18995271623134613,
-0.008308811113238335,
0.030797995626926422,
-0.0047542075626552105,
-0.023831071332097054,
-0.05116206035017967,
-0.013412323780357838,
-0.008527606725692749,
0.031027967110276222,
0.006126040127128363,
0.04799506440758705,
-0.007955936715006828,
-0.025750210508704185,
-0.012767388485372066,
0.08466999977827072,
0.033956129103899,
0.008220010437071323,
-0.012998132035136223,
-0.03014502115547657,
0.062383465468883514,
-0.00174158054869622,
-0.020453618839383125,
-0.01712154597043991,
-0.004657802637666464,
-0.003376249223947525,
0.0015114082489162683,
-0.013574525713920593,
0.0059362719766795635,
0.04020567610859871,
0.04541381821036339,
-0.03138380125164986,
-0.002565910341218114,
0.022982968017458916,
-0.15856429934501648,
0.029680028557777405,
0.047427743673324585,
-0.01921428181231022,
0.0023907183203846216,
-0.043014995753765106,
0.025887714698910713,
0.07320819050073624,
-0.07492371648550034,
0.01687679812312126,
-0.016796093434095383,
-0.028022760525345802,
-0.020995832979679108,
-0.02225467748939991,
-0.014568421989679337,
0.016813112422823906,
0.04094502702355385,
0.047387268394231796,
-0.02681197039783001,
0.023330409079790115,
-0.010400434955954552,
-0.030882740393280983,
-0.03135886415839195,
0.023597734048962593,
0.009329996071755886,
-0.005194028373807669,
-0.01537210401147604,
0.06594531983137131,
0.007914959453046322,
-0.03371259942650795,
-0.029784061014652252,
-0.028193118050694466,
-0.06191715970635414,
0.09086465835571289,
0.049846649169921875,
0.02329147234559059,
0.005298103671520948,
0.36908629536628723,
-0.0561729334294796,
0.02429560385644436,
-0.11446176469326019,
0.02156238630414009,
-0.018498582765460014,
-0.04236149042844772,
-0.005646711681038141,
-0.006741815246641636,
-0.03543572872877121,
0.05371907725930214,
0.13205832242965698,
-0.039469391107559204,
-0.004171531181782484,
-0.0674717128276825,
-0.028708800673484802,
-0.03984411433339119,
0.025505412369966507,
0.045827120542526245,
-0.06408290565013885,
0.047309838235378265,
0.03499940037727356,
-0.025898486375808716,
0.029317723587155342,
-0.04448636248707771,
0.02448771335184574,
0.04939476400613785,
-0.08074253797531128,
0.0946812555193901,
0.027259016409516335,
0.06017233803868294,
0.006169850938022137,
0.024212686344981194,
0.02260514907538891,
0.025200610980391502,
-0.021984897553920746,
-0.037586312741041183,
0.07039269059896469,
-0.03340630605816841,
0.05132792517542839,
-0.015640713274478912,
-0.019254570826888084,
0.021525269374251366,
-0.0590442456305027,
-0.06896568834781647,
0.01237141340970993,
0.07801492512226105,
-0.02763948403298855,
0.01826077327132225,
0.021632501855492592,
-0.013012842275202274,
-0.037968967109918594,
-0.004417243413627148,
-0.008026628755033016,
0.05424785241484642,
-0.062349747866392136,
0.051168397068977356,
-0.028916290029883385,
-0.008591882884502411,
0.014335441403090954,
-0.042277880012989044,
-0.09226332604885101,
0.014696937054395676,
0.01875472068786621,
0.008640816435217857,
0.021558547392487526,
0.039570402354002,
-0.007988952100276947,
-0.01852112077176571,
-0.023714732378721237,
-0.0010199069511145353,
-0.03425934910774231,
0.03720324486494064,
-0.005075500346720219,
-0.05304834619164467,
-0.042861755937337875,
-0.015467766672372818,
0.05941595509648323,
0.028385918587446213,
0.014841306954622269,
-0.0793217346072197,
-0.003600947791710496,
-0.06225157901644707,
0.00023353227879852057,
-0.010698274709284306,
0.011295401491224766,
-0.08222774416208267,
0.02788129821419716,
0.04051422327756882,
0.0022866211365908384,
0.0612201914191246,
-0.012599431909620762,
0.01876194030046463,
-0.08027555793523788,
0.00420914962887764,
-0.05815249681472778,
-0.09123749285936356,
-0.031796108931303024,
0.02535978890955448,
-0.0006155986920930445,
-0.004111003130674362,
0.12450843304395676,
0.07037623226642609,
0.02785305306315422,
0.08709102123975754,
-0.010294836945831776,
0.04579058289527893,
-0.056833188980817795,
-0.0005778326885774732,
0.05766087397933006,
0.020506851375102997,
-0.07668095827102661,
0.014184726402163506,
-0.022567380219697952,
0.014976873993873596,
-0.05125178396701813,
0.023779619485139847,
-0.01692485250532627,
-0.014204083941876888,
-0.003975256811827421,
-0.03782648220658302,
0.03667180612683296,
-0.04059094563126564,
-0.0047954535111784935,
-0.31382203102111816,
-0.08827133476734161,
0.001990135759115219,
0.02284920960664749,
-0.004349472466856241,
-0.01081603579223156,
0.05062827840447426,
0.006937489379197359,
-0.03258923068642616,
-0.02445329912006855,
0.023143984377384186,
0.02939695492386818,
-0.032841384410858154,
-0.01429196447134018,
-0.005593874957412481,
0.015684137120842934,
-0.00611750828102231,
-0.038208600133657455,
0.02668623812496662,
0.02181783877313137,
0.016270821914076805,
-0.03661072999238968,
0.01467888429760933,
-0.016118809580802917,
0.03693212568759918,
0.012551719322800636,
0.05371477082371712,
0.010337740182876587,
-0.014881397597491741,
-0.05765775963664055,
0.06245936453342438,
0.10514939576387405,
0.013108562678098679,
-0.09487926214933395,
0.037743035703897476,
-0.015905948355793953,
-0.008098716847598553,
-0.036317843943834305,
0.011239915154874325,
0.014345540665090084,
-0.0484619177877903,
0.04018568992614746,
-0.05902618542313576,
-0.06453020870685577,
-0.012686937116086483,
-0.016964776441454887,
-0.019501132890582085,
0.04172061011195183,
-0.03958355635404587,
-0.008335338905453682,
0.05958887189626694,
0.05575687810778618,
0.05318684130907059,
-0.04311138391494751,
0.012993976473808289,
0.006258177105337381,
-0.0058147842064499855,
0.022125542163848877,
-0.04794088006019592,
0.016491645947098732,
-0.06599736213684082,
0.03971907123923302,
-0.01015787199139595,
0.05681545287370682,
0.008375116623938084,
-0.015018514357507229,
-0.03176276758313179,
-0.06073540821671486,
0.04348607733845711,
0.032225534319877625,
0.05133874714374542,
0.11220784485340118,
-0.05374489352107048,
0.02100866474211216,
0.039634473621845245,
0.02798665128648281,
-0.07484667003154755,
-0.013223299756646156,
-0.065217524766922,
0.019543470814824104,
0.0360308401286602,
-0.012478767894208431,
0.06471627205610275,
-0.019087951630353928,
-0.048421356827020645,
0.06946633011102676,
0.05424578860402107,
0.017972372472286224,
0.04145842045545578,
-0.02115762047469616,
0.0020777981262654066,
0.008108225651085377,
-0.01764906942844391,
-0.005943856202065945,
0.05608458071947098,
0.03555358201265335,
-0.2472069263458252,
0.019470779225230217,
0.017575504258275032,
-0.022291118279099464,
-0.05028887465596199,
0.08393196761608124,
0.02159504033625126,
0.022756418213248253,
-0.022055385634303093,
0.035847388207912445,
0.04517258703708649,
-0.0015784172574058175,
0.051570091396570206,
0.046219464391469955,
-0.010045139119029045,
0.03352583944797516,
0.0341263972222805,
-0.029658105224370956,
0.030433783307671547,
-0.06796430796384811,
0.023406075313687325,
0.0002016468351939693,
0.13622306287288666,
-0.013408143073320389,
-0.018998250365257263,
0.039640869945287704,
0.03709225729107857,
0.021389693021774292,
-0.07353408634662628,
0.015692871063947678,
-0.04095860570669174,
0.01435636356472969,
0.0920715183019638,
-0.051623303443193436,
-0.010256427340209484,
0.07241562008857727,
-0.00608614319935441,
0.037644971162080765,
-0.028301218524575233,
0.009015488438308239,
-0.0010027086827903986,
0.012127600610256195,
-0.06752531230449677,
0.0076929423958063126,
0.05544263869524002,
-0.0358983539044857,
0.013529481366276741,
-0.05808970332145691,
-0.0006077744183130562,
-0.025475626811385155,
-0.009186177514493465,
-0.06575009226799011,
0.024258989840745926,
0.031344737857580185,
-0.008625148795545101,
-0.04455156996846199,
-0.006473247427493334,
-0.009557497687637806,
0.0018567965598776937,
0.053140465170145035,
0.036117374897003174,
0.030359243974089622,
0.0031315062660723925,
0.07577982544898987,
0.034163929522037506
] |
PMID:18750
|
Antagonism of the analeptic activity of thyrotropin-releasing hormone (TRH) by agents which enhance GABA transmission.
|
Administration of 10 mg/kg TRH to mice was found to reduce the sleep and hypothermia induced by 4.7 g/kg ethanol. However, TRH did not reduce the sleep of mice that were given gamma-hydroxybutyric acid (GHBA), baclophen, or aminooxyacetic acid (AOAA) in combination with 3 g/kg/ ethanol. TRH also failed to reverse the hypothermia induced by the combination of ethanol and baclophen or GHBA, and the characteristic neurological effects of TRH e.g. tremor, increased muscle tone, and increased respiratory rate were reduced. In addition, TRH-induced locomotor stimulation was prevented by pretreatment with small doses of the GABA-ergic agents, and while 30 mg/kg TRH reduced the hypothermia produced by large doses of the GABA-ergic drugs, it did not antagonize the locomotor retardation produced by baclophen or GHBA. A hypothesis that the analeptic effects of TRH may be medicated via an inhibition of GABA systems is discussed.
|
Antagonism of the analeptic activity of thyrotropin-releasing hormone (TRH) by agents which enhance GABA transmission. Administration of 10 mg/kg TRH to mice was found to reduce the sleep and hypothermia induced by 4.7 g/kg ethanol. However, TRH did not reduce the sleep of mice that were given gamma-hydroxybutyric acid (GHBA), baclophen, or aminooxyacetic acid (AOAA) in combination with 3 g/kg/ ethanol. TRH also failed to reverse the hypothermia induced by the combination of ethanol and baclophen or GHBA, and the characteristic neurological effects of TRH e.g. tremor, increased muscle tone, and increased respiratory rate were reduced. In addition, TRH-induced locomotor stimulation was prevented by pretreatment with small doses of the GABA-ergic agents, and while 30 mg/kg TRH reduced the hypothermia produced by large doses of the GABA-ergic drugs, it did not antagonize the locomotor retardation produced by baclophen or GHBA. A hypothesis that the analeptic effects of TRH may be medicated via an inhibition of GABA systems is discussed.
|
[
0.017348112538456917,
0.041779033839702606,
0.002707596868276596,
0.057046078145504,
0.005828436464071274,
0.026159295812249184,
0.026255495846271515,
0.02302699349820614,
-0.07357475906610489,
-0.037949755787849426,
-0.044389739632606506,
-0.036059148609638214,
-0.0013452810235321522,
0.06581861525774002,
0.002977765630930662,
-0.0009306438150815666,
0.057315390557050705,
-0.01880023069679737,
-0.030701959505677223,
-0.00036400248063728213,
-0.03155025839805603,
0.014393825083971024,
0.05126544088125229,
0.014965411275625229,
0.00020466317073442042,
0.021723272278904915,
-0.07907503843307495,
0.017107302322983742,
-0.0415695495903492,
-0.15267977118492126,
0.032563820481300354,
-0.001308306003920734,
0.02130316011607647,
-0.027117621153593063,
-0.05699637159705162,
0.017309948801994324,
0.014338298700749874,
0.005360778886824846,
-0.017323892563581467,
-0.007910152897238731,
0.06734608113765717,
0.018717119470238686,
-0.013467704877257347,
0.00811021588742733,
0.046068690717220306,
0.06287947297096252,
-0.010079136118292809,
0.012278991751372814,
0.06427720189094543,
0.009641236625611782,
-0.0004065895627718419,
0.0033306730911135674,
0.04386318475008011,
0.05199059098958969,
-0.00812966376543045,
-0.05333014950156212,
-0.01693248748779297,
0.05887638404965401,
-0.03472068905830383,
0.06261349469423294,
0.017993636429309845,
0.022577941417694092,
-0.12635301053524017,
0.03444899246096611,
0.0712488517165184,
0.011564095504581928,
-0.03819300979375839,
-0.042251478880643845,
0.038388073444366455,
0.06616444140672684,
-0.06663370877504349,
0.028879236429929733,
0.025728246197104454,
-0.007038477808237076,
-0.011772478930652142,
-0.02663593739271164,
0.0008000883972272277,
-0.024256693199276924,
0.036689188331365585,
-0.0035960080567747355,
0.055212367326021194,
-0.0009463601745665073,
0.07651518285274506,
-0.009647718630731106,
0.0042535411193966866,
-0.03318113088607788,
-0.00594757916405797,
-0.05217277258634567,
-0.020326340571045876,
-0.002274655271321535,
0.019727716222405434,
-0.04964158311486244,
-0.020578360185027122,
-0.01125489640980959,
-0.018068090081214905,
0.01836729608476162,
0.007577185053378344,
-0.05359933525323868,
-0.028307940810918808,
0.3296460509300232,
0.010867766104638577,
0.026758763939142227,
-0.07112386077642441,
-0.06765595823526382,
0.024038556963205338,
-0.0007892864523455501,
0.0005408062716014683,
-0.023198239505290985,
-0.05166659504175186,
0.0723414421081543,
0.02959783747792244,
-0.036269694566726685,
0.0354795828461647,
-0.02910076640546322,
-0.016749195754528046,
-0.016957445070147514,
0.07978057861328125,
0.021185575053095818,
0.004698121454566717,
-0.0268595889210701,
-0.042556263506412506,
-0.018807794898748398,
0.08716204762458801,
-0.023485571146011353,
0.00809725746512413,
-0.021094923838973045,
-0.022780336439609528,
0.11309218406677246,
0.06610452383756638,
0.0013075737515464425,
0.06485812366008759,
-0.021572018042206764,
0.049809180200099945,
0.023091677576303482,
0.030699793249368668,
0.032895155251026154,
0.028188252821564674,
-0.09120918065309525,
0.0012456764234229922,
0.007873822003602982,
0.01737561821937561,
-0.037863194942474365,
0.03151686489582062,
-0.08814418315887451,
-0.058328572660684586,
0.06213374063372612,
0.018434764817357063,
-0.018277741968631744,
-0.02603093348443508,
0.03738785162568092,
0.039675306528806686,
0.011760836467146873,
0.007378042675554752,
0.0425659716129303,
-0.03165430948138237,
0.075722336769104,
-0.004633549600839615,
0.05269738659262657,
-0.03091222606599331,
0.01867535337805748,
-0.06470713019371033,
-0.023912537842988968,
0.005077844951301813,
0.004537671338766813,
-0.008948919363319874,
0.020008541643619537,
-0.037169743329286575,
-0.006807413883507252,
-0.04297026991844177,
0.016320522874593735,
-0.01803027093410492,
0.07359446585178375,
-0.03528369590640068,
-0.020364349707961082,
-0.006621135398745537,
-0.008733604103326797,
0.057340074330568314,
-0.017793217673897743,
0.029400307685136795,
-0.04304968938231468,
0.038595255464315414,
-0.062046490609645844,
-0.00451976852491498,
0.015196455642580986,
0.06419201195240021,
0.0266766045242548,
-0.05028726905584335,
0.032173626124858856,
-0.020892512053251266,
0.013523510657250881,
-0.07512902468442917,
-0.021112190559506416,
-0.03615088760852814,
-0.05392611771821976,
-0.020110689103603363,
-0.06800547987222672,
-0.03108443133533001,
0.008728104643523693,
-0.004658219870179892,
0.06362906843423843,
0.05089510232210159,
0.05574169382452965,
-0.01975945197045803,
0.05629318952560425,
0.022850828245282173,
0.05377540737390518,
-0.04524923861026764,
-0.03023708425462246,
0.055710319429636,
-0.05671178549528122,
-0.025182614102959633,
-0.005377164110541344,
-0.015899157151579857,
-0.011004150845110416,
-0.07167172431945801,
0.08626629412174225,
-0.02040768228471279,
-0.0011984787415713072,
-0.0024603833444416523,
0.01688019558787346,
0.053782083094120026,
-0.08502890169620514,
0.0255836583673954,
-0.3349637985229492,
-0.05314368009567261,
-0.0066048577427864075,
-0.03574691340327263,
0.03611984848976135,
-0.08270277827978134,
-0.012606731615960598,
-0.06751207262277603,
-0.02553470805287361,
-0.0020161557476967573,
0.06160999834537506,
0.0988260805606842,
-0.0769459456205368,
-0.04298333451151848,
-0.004409870598465204,
0.04496143385767937,
0.00922871008515358,
-0.0476214662194252,
0.06460036337375641,
-0.032200589776039124,
0.033654529601335526,
-0.015539480373263359,
-0.050533927977085114,
0.002738791285082698,
0.027513818815350533,
-0.01431274600327015,
0.10583595186471939,
0.012006950564682484,
0.02108147367835045,
-0.06390094757080078,
0.009695921093225479,
0.05123712867498398,
-0.0020082960836589336,
-0.08127836138010025,
0.02702660672366619,
0.007191361393779516,
0.001775384065695107,
0.0019181650131940842,
0.03178800269961357,
-0.03239341080188751,
-0.0847734659910202,
0.007766060996800661,
-0.038057226687669754,
-0.09953117370605469,
-0.09103873372077942,
0.008468626998364925,
0.02504831738770008,
-0.02335919253528118,
0.024481767788529396,
-0.04108549281954765,
0.07615025341510773,
0.044971294701099396,
0.013334582559764385,
-0.029582733288407326,
0.03227102756500244,
-0.03367209807038307,
-0.049403876066207886,
0.0003658497880678624,
-0.01903819851577282,
0.004217167850583792,
0.012575558386743069,
0.05909925699234009,
0.02734462358057499,
0.02677951380610466,
-0.0065699052065610886,
-0.01873479038476944,
-0.02789335697889328,
0.0015090398956090212,
0.08516938239336014,
0.08913221210241318,
-0.006482970900833607,
0.11478934437036514,
0.010659024119377136,
0.035422809422016144,
0.002113129710778594,
-0.0208299458026886,
-0.03722776845097542,
-0.05220705270767212,
-0.08046245574951172,
0.053347136825323105,
-0.06831484287977219,
-0.024167001247406006,
0.09019631147384644,
0.01067885011434555,
-0.04139093682169914,
-0.021976634860038757,
0.01786968484520912,
-0.0011209020158275962,
0.08557718992233276,
-0.03968724235892296,
0.013361834920942783,
0.0028543672524392605,
0.021036619320511818,
-0.05177077651023865,
0.04426250979304314,
0.04207094758749008,
-0.2565588653087616,
-0.00271948566660285,
-0.014885305427014828,
-0.03464869409799576,
0.021826155483722687,
0.03446332365274429,
-0.005206449888646603,
-0.019595036283135414,
-0.05956771969795227,
0.009546511806547642,
-0.06340095400810242,
0.026604169979691505,
0.011515474878251553,
0.08261659741401672,
-0.0038856826722621918,
-0.050721488893032074,
0.05588791146874428,
-0.04218991845846176,
-0.00934899877756834,
0.03759992867708206,
0.005531164817512035,
0.015624667517840862,
0.13772635161876678,
0.04157061502337456,
0.024565760046243668,
0.02448417991399765,
0.0017618875717744231,
0.03897063806653023,
-0.017053760588169098,
-0.018550312146544456,
-0.056688543409109116,
-0.02268722653388977,
0.08165397495031357,
-0.016687551513314247,
0.08030632138252258,
-0.0022564646787941456,
-0.08804846554994583,
0.022839877754449844,
-0.0009040581644512713,
0.04716835543513298,
-0.03940882161259651,
0.03521115332841873,
-0.02250434085726738,
-0.04074625298380852,
0.12319567799568176,
-0.02741796150803566,
-0.03388699144124985,
-0.03447897732257843,
-0.050759587436914444,
0.03679043427109718,
-0.0038772730622440577,
-0.0172512698918581,
-0.0007017955649644136,
0.019446751102805138,
-0.002868285868316889,
0.003283956553786993,
-0.0025099923368543386,
0.014152981340885162,
0.009935211390256882,
-0.009305639192461967,
-0.004292605444788933,
-0.05975356325507164,
-0.06486111134290695,
0.08688946068286896,
0.009908657521009445
] |
PMID:18751
|
Benzodiazepines and discrimination behaviour: dissociation of response and sensory factors.
|
Ten London pigeons were trained on a schedule which allowed concurrent measurement of motor and exteroceptive sensory changes. The task involved a conditional colour discrimination contingent upon the completion of a fixed interval schedule of responding. As a preliminary pharmacological study the effects of the benzodiazepines, chlordiazepoxide and flurazepam were investigated. Both drugs enhanced perseverative responding (after completion of the FI), and to a variable degree responding during the FI, although there was no evidence for an increase in responding during the inter-trial-intervals. On the other hand, no marked changes in discrimination performance were observed. It is concluded that the most significant effects of these benzodiazepines are on motor mechanisms.
|
Benzodiazepines and discrimination behaviour: dissociation of response and sensory factors. Ten London pigeons were trained on a schedule which allowed concurrent measurement of motor and exteroceptive sensory changes. The task involved a conditional colour discrimination contingent upon the completion of a fixed interval schedule of responding. As a preliminary pharmacological study the effects of the benzodiazepines, chlordiazepoxide and flurazepam were investigated. Both drugs enhanced perseverative responding (after completion of the FI), and to a variable degree responding during the FI, although there was no evidence for an increase in responding during the inter-trial-intervals. On the other hand, no marked changes in discrimination performance were observed. It is concluded that the most significant effects of these benzodiazepines are on motor mechanisms.
|
[
-0.025966156274080276,
-0.007212631870061159,
0.04755256697535515,
0.07038431614637375,
0.02002454735338688,
0.033922165632247925,
0.0802081972360611,
0.017054859548807144,
-0.002769723767414689,
-0.056584928184747696,
-0.04006459191441536,
-0.012481065467000008,
-0.033560559153556824,
0.025096110999584198,
-0.01695670560002327,
0.002646413166075945,
0.016222255304455757,
-0.04233359917998314,
-0.0721702128648758,
-0.01688980683684349,
-0.07282528281211853,
0.006335042417049408,
0.025085683912038803,
0.007525585126131773,
-0.00435047410428524,
-0.011884144507348537,
-0.02371317334473133,
0.017995530739426613,
-0.03503336384892464,
-0.1645958572626114,
0.04227513074874878,
0.002697918564081192,
0.017367415130138397,
-0.08896462619304657,
-0.02055410109460354,
0.004501344170421362,
0.01433586422353983,
0.0416005440056324,
-0.04993673413991928,
0.02337888441979885,
0.06918644905090332,
0.026874149218201637,
-0.0635402575135231,
-0.0056266095489263535,
-0.020541692152619362,
-0.02982250414788723,
-0.015351726673543453,
-0.05712246522307396,
0.023244548588991165,
-0.09073459357023239,
0.005093494895845652,
-0.09732016175985336,
0.05079836770892143,
0.04476340860128403,
-0.030339796096086502,
0.03616347163915634,
-0.012320911511778831,
0.014734272845089436,
-0.054879508912563324,
0.02510784938931465,
-0.0038639595732092857,
0.010234244167804718,
-0.14040935039520264,
0.020666155964136124,
0.00017337313329335302,
0.023045208305120468,
-0.025410121306777,
-0.02867875248193741,
0.03329931199550629,
0.07921859622001648,
-0.09012994170188904,
0.010457934811711311,
0.020506244152784348,
-0.020653389394283295,
0.04664110764861107,
-0.0304839089512825,
-0.01873048208653927,
-0.04540131241083145,
-0.02520669251680374,
-0.08399932831525803,
0.0330934040248394,
0.008646631613373756,
-0.009358244016766548,
-0.049891021102666855,
0.036283913999795914,
-0.02057529054582119,
0.06884299218654633,
-0.004346017260104418,
0.042104024440050125,
0.02082323282957077,
0.05763952061533928,
-0.024240553379058838,
0.001563973375596106,
-0.07933317869901657,
0.027800902724266052,
0.04325519874691963,
0.01680563949048519,
-0.05456634610891342,
-0.010269749909639359,
0.3977147340774536,
0.026370946317911148,
0.03579993546009064,
-0.04133674502372742,
-0.021277908235788345,
0.030878478661179543,
0.007435410283505917,
0.048939161002635956,
0.0020037484355270863,
-0.062066614627838135,
0.041797392070293427,
0.03546329215168953,
-0.05231846496462822,
0.06538508832454681,
0.051324762403964996,
-0.06129040569067001,
0.0041733114048838615,
0.01847895234823227,
-0.014055903069674969,
0.07964123040437698,
0.02913646399974823,
0.01629708707332611,
-0.003111724741756916,
0.054266493767499924,
-0.007053175941109657,
0.07946986705064774,
-0.012913581915199757,
-0.029260631650686264,
0.11688549816608429,
-0.03174535557627678,
-0.05238828808069229,
0.061936404556035995,
-0.07037605345249176,
0.06422631442546844,
0.054782114923000336,
0.0013933549635112286,
0.042602308094501495,
0.08749288320541382,
-0.018399950116872787,
0.021869273856282234,
0.00559853482991457,
0.01871354691684246,
-0.030971407890319824,
0.03265053406357765,
-0.08516604453325272,
-0.031394343823194504,
0.10427845269441605,
0.020659230649471283,
-0.058691781014204025,
-0.04537209868431091,
0.06418364495038986,
-0.07393969595432281,
-0.008525602519512177,
-0.008718864060938358,
0.036590080708265305,
-0.08015693724155426,
0.04507730156183243,
0.0024396919179707766,
0.030701328068971634,
0.01675383932888508,
0.015411747619509697,
-0.02165336161851883,
-0.03509959951043129,
0.01229989156126976,
0.0886387825012207,
0.006675968877971172,
0.015315418131649494,
-0.03863365948200226,
-0.029293330386281013,
-0.04934412240982056,
0.03853195905685425,
-0.001623269054107368,
0.01803852990269661,
-0.03480583801865578,
-0.017887186259031296,
-0.05115124583244324,
-0.030157700181007385,
0.002756509929895401,
0.02419350855052471,
-0.02873038314282894,
0.015081420540809631,
0.05674513801932335,
-0.03063609078526497,
-0.01916995644569397,
0.011437860317528248,
0.014419380575418472,
0.03662429377436638,
-0.0167704988270998,
0.014940096065402031,
-0.005817907862365246,
0.014013178646564484,
-0.025193162262439728,
-0.029831653460860252,
-0.008079023100435734,
-0.06316569447517395,
-0.07749317586421967,
-0.06547286361455917,
-0.06744684278964996,
0.006236318964511156,
-0.01632862724363804,
-0.0018289085710421205,
0.06613726913928986,
0.027005113661289215,
0.004140111152082682,
0.03916792571544647,
0.045076917856931686,
0.050161320716142654,
-0.005836698226630688,
-0.010196668095886707,
0.08796682953834534,
0.007300341967493296,
0.0041352384723722935,
-0.020151827484369278,
0.03987349942326546,
0.023281311616301537,
-0.023974010720849037,
0.041443318128585815,
-0.06049487367272377,
0.03315971419215202,
0.021512726321816444,
0.03351317718625069,
-0.004181492608040571,
-0.027579646557569504,
-0.010183488950133324,
-0.2906801998615265,
-0.0449179969727993,
-0.002840118482708931,
0.010171202011406422,
0.05513491481542587,
-0.021134549751877785,
0.019008345901966095,
-0.05394434183835983,
0.01163917500525713,
-0.013564600609242916,
-0.020621923729777336,
0.008370248600840569,
-0.048576585948467255,
0.015689048916101456,
0.00324240536428988,
0.012438432313501835,
0.025006532669067383,
-0.047038689255714417,
-0.006630452815443277,
0.004860774613916874,
-0.01847008615732193,
0.010875360108911991,
-0.020927410572767258,
0.012985065579414368,
0.03182089328765869,
-0.003415005747228861,
0.1117735356092453,
0.016800856217741966,
0.008253968320786953,
-0.007955492474138737,
-0.012879139743745327,
0.08091564476490021,
-0.01616782881319523,
-0.10121903568506241,
0.046891551464796066,
0.038991473615169525,
-0.03691164776682854,
0.0057984087616205215,
-0.0003048635262530297,
-0.03199297562241554,
-0.05845548212528229,
0.03192789480090141,
0.009865355677902699,
-0.027486756443977356,
-0.11401788145303726,
0.005410338286310434,
0.03349635377526283,
-0.004794111009687185,
-0.03952173888683319,
-0.0058253915049135685,
-0.0046606240794062614,
0.04668954387307167,
0.02240915596485138,
-0.016433624550700188,
0.01112109050154686,
-0.02506917528808117,
-0.09077732264995575,
0.025739843025803566,
-0.0672045350074768,
0.0044759283773601055,
0.08674171566963196,
-0.005411962047219276,
0.036858268082141876,
-0.05942955240607262,
-0.007908515632152557,
0.058498483151197433,
-0.0024473113007843494,
-0.0444691926240921,
0.05592603236436844,
0.05172371491789818,
-0.035951096564531326,
0.13139674067497253,
-0.003058933885768056,
-0.035526856780052185,
0.08449620753526688,
-0.018042581155896187,
-0.032475151121616364,
-0.0198796596378088,
-0.07380998134613037,
0.0034990531858056784,
0.004754043649882078,
-0.014964253641664982,
0.04102572053670883,
0.025955818593502045,
-0.015097696334123611,
-0.03234022483229637,
0.014569655060768127,
-0.006258342415094376,
0.07189295440912247,
0.023338470607995987,
0.0011334148002788424,
-0.005167277995496988,
0.008549013175070286,
-0.03552673012018204,
0.044408801943063736,
0.06625557690858841,
-0.2554142475128174,
0.013613821938633919,
0.020447824150323868,
0.08011279255151749,
0.018259476870298386,
0.04434017091989517,
0.02959286794066429,
-0.03617844358086586,
-0.025578942149877548,
-0.04325835406780243,
-0.027030229568481445,
0.00032083565020002425,
0.021971682086586952,
0.06618250906467438,
-0.06208030879497528,
0.018489442765712738,
0.06630364805459976,
-0.0038104509003460407,
0.014163210988044739,
0.00020079599926248193,
0.005630985833704472,
0.0609615221619606,
0.136543869972229,
0.032989244908094406,
0.028969792649149895,
0.006403337698429823,
0.015536822378635406,
-0.04879894480109215,
-0.006470971275120974,
-0.02414654567837715,
0.02742134779691696,
-0.001472885487601161,
0.07380010187625885,
-0.039983119815588,
-0.007295139133930206,
0.008939290419220924,
-0.06029348075389862,
-0.025698931887745857,
0.010257593356072903,
0.013169771991670132,
-0.008005216717720032,
0.0066879247315227985,
-0.0030001471750438213,
-0.039103493094444275,
0.09483807533979416,
-0.03968777135014534,
0.004925563000142574,
-0.07827906310558319,
-0.02903532236814499,
0.012376303784549236,
-0.029679715633392334,
-0.0534607395529747,
-0.030026482418179512,
0.004368678666651249,
0.042704544961452484,
-0.01537676714360714,
-0.02002091333270073,
-0.017412303015589714,
0.05169128254055977,
-0.0007849426474422216,
0.011750367470085621,
-0.003429670352488756,
0.00828976184129715,
0.011431446298956871,
-0.014114606194198132
] |
PMID:18752
|
Effect of apomorphine on the antinociceptive activity of morphine.
|
Apomorphine pretreatment potentiated the analgesic effect of morphine in a dose-dependent manner both in rats and in mice measured by five different tests (writhing, hot plate, inflamed foot, tail-pinch and tail-flick procedures). Furthermore, apomorphine augmented the antinociceptive activity of morphine in tolerant animals as well. In morphine dependent mice the nalorphine precipitated jumping--a withdrawal symptom--was found inhibited by apomorphine treatment. The results are discussed in the light of the numerous but contradictory data available in the literature.
|
Effect of apomorphine on the antinociceptive activity of morphine. Apomorphine pretreatment potentiated the analgesic effect of morphine in a dose-dependent manner both in rats and in mice measured by five different tests (writhing, hot plate, inflamed foot, tail-pinch and tail-flick procedures). Furthermore, apomorphine augmented the antinociceptive activity of morphine in tolerant animals as well. In morphine dependent mice the nalorphine precipitated jumping--a withdrawal symptom--was found inhibited by apomorphine treatment. The results are discussed in the light of the numerous but contradictory data available in the literature.
|
[
0.007599205709993839,
-0.046133507043123245,
0.020530827343463898,
0.07520376145839691,
0.0003135611186735332,
0.0026938719674944878,
0.07337372750043869,
0.07272578775882721,
-0.046908728778362274,
-0.03337080031633377,
-0.019511111080646515,
0.038064904510974884,
-0.028169577941298485,
0.044167205691337585,
-0.04741061478853226,
0.0438055656850338,
0.021039210259914398,
-0.01245355699211359,
-0.06005135923624039,
-0.0008163127349689603,
-0.034145109355449677,
-0.029243819415569305,
0.0054768449626863,
0.0033827750012278557,
-0.025549182668328285,
0.01883445680141449,
0.055321309715509415,
0.022036906331777573,
-0.05780363827943802,
-0.13994961977005005,
-0.022565601393580437,
-0.009435219690203667,
0.05005944147706032,
-0.05134281888604164,
-0.020050888881087303,
0.0795489102602005,
-0.01602337509393692,
0.03168661892414093,
-0.029366016387939453,
0.06592057645320892,
0.03297405317425728,
0.03089742548763752,
-0.02206883765757084,
0.0347001813352108,
0.04704569652676582,
-0.0291427094489336,
-0.03507506102323532,
-0.07958689332008362,
0.10001987218856812,
0.021888190880417824,
0.0620662160217762,
-0.07368280738592148,
0.015918763354420662,
0.06686210632324219,
0.049286000430583954,
-0.03272264078259468,
-0.037041280418634415,
0.03702510893344879,
-0.03848212584853172,
0.06467188894748688,
0.0008291288977488875,
0.03496997430920601,
-0.13205762207508087,
0.01883016899228096,
0.04931405186653137,
-0.04075400158762932,
-0.05299925431609154,
-0.042213767766952515,
0.04440420866012573,
0.11461403220891953,
-0.10812650620937347,
0.04467078670859337,
0.031127668917179108,
0.010219941847026348,
0.020501794293522835,
-0.030099403113126755,
0.006775411311537027,
-0.008618451654911041,
0.015470892190933228,
-0.00180491351056844,
0.0925147607922554,
-0.007880310527980328,
-0.02215898223221302,
-0.06694924831390381,
0.002869312884286046,
-0.09947291761636734,
0.009712481871247292,
-0.01337951235473156,
-0.004528630990535021,
-0.01143643632531166,
-0.01203692052513361,
0.008999827317893505,
-0.03476516157388687,
-0.030054958537220955,
-0.042293038219213486,
-0.007832595147192478,
0.01314906682819128,
-0.02244357205927372,
0.011515125632286072,
0.3349258601665497,
-0.02382233925163746,
0.02010350301861763,
-0.08400122821331024,
-0.01990455575287342,
-0.005737278610467911,
0.0027237115427851677,
0.010195869021117687,
0.027046963572502136,
-0.03962000086903572,
0.08049660921096802,
0.050457194447517395,
0.028553513810038567,
0.045202646404504776,
0.04347584769129753,
-0.01744770258665085,
-0.06125280261039734,
0.034257322549819946,
0.02406160905957222,
0.03889022767543793,
0.011094351299107075,
0.0007094612228684127,
-0.044506900012493134,
0.047856397926807404,
0.019246872514486313,
-0.007540736347436905,
0.01740415394306183,
-0.0024015309754759073,
0.08350730687379837,
0.019546175375580788,
-0.0016533746384084225,
0.022353261709213257,
-0.09945593774318695,
0.029824690893292427,
0.029582109302282333,
0.02495942823588848,
-0.018932906910777092,
0.044917989522218704,
-0.013320757076144218,
0.022840529680252075,
0.005438892636448145,
0.0178760327398777,
-0.06030745059251785,
0.011054052971303463,
-0.046635713428258896,
-0.03359214588999748,
0.055879563093185425,
0.02740516886115074,
-0.006456410046666861,
0.016696441918611526,
0.0617474727332592,
-0.029861200600862503,
0.020387684926390648,
-0.030830862000584602,
0.045564111322164536,
-0.04353785887360573,
0.0628020390868187,
-0.008255611173808575,
-0.03356165811419487,
-0.00835869088768959,
0.004357390105724335,
-0.03285958245396614,
0.02036653272807598,
-0.015513758175075054,
0.0671234279870987,
-0.007159791886806488,
0.050553373992443085,
-0.0343804769217968,
0.01853572390973568,
-0.040720634162425995,
-0.004597475752234459,
-0.02758171036839485,
0.0035678751301020384,
-0.06183989346027374,
-0.1219734251499176,
-0.03565823286771774,
0.024338379502296448,
0.05733630806207657,
-0.023750899359583855,
0.003154621459543705,
-0.02642936445772648,
0.010596966370940208,
-0.010736541822552681,
0.015623340383172035,
0.0030266642570495605,
-0.02018640749156475,
0.008289319463074207,
-0.02010638825595379,
0.002238260582089424,
0.018121935427188873,
-0.033607155084609985,
0.01088385097682476,
-0.06070410832762718,
-0.11721599102020264,
-0.0658721849322319,
-0.019657140597701073,
-0.02316880412399769,
-0.005713154096156359,
0.054988689720630646,
-0.01152881234884262,
0.010256494395434856,
0.029109405353665352,
0.060394395142793655,
0.0245088841766119,
0.028564568608999252,
0.017878295853734016,
0.045296382158994675,
0.011409599334001541,
0.027161981910467148,
-0.015477056615054607,
-0.04154401272535324,
-0.025288887321949005,
0.02692451886832714,
0.05069208890199661,
0.021407566964626312,
-0.05391135439276695,
0.08922412246465683,
-0.015311911702156067,
-0.0073591009713709354,
0.07227081060409546,
0.02338768169283867,
-0.027999592944979668,
-0.05168033763766289,
0.05117793381214142,
-0.2921205759048462,
0.0023072578478604555,
0.007650094572454691,
0.015119938179850578,
0.06042361259460449,
-0.04187726229429245,
0.02871159091591835,
-0.0726654902100563,
-0.003712255274876952,
-0.06254028528928757,
0.06030973792076111,
0.03195427730679512,
-0.007259259466081858,
0.0034036634024232626,
0.046001095324754715,
0.034343548119068146,
0.008912733756005764,
-0.001139750238507986,
0.03500140830874443,
0.03248702362179756,
0.020319225266575813,
-0.011325914412736893,
-0.001604558085091412,
-0.02193595841526985,
-0.0035495234187692404,
-0.0577557347714901,
0.07500322163105011,
0.05551046505570412,
-0.06712139397859573,
0.013889157213270664,
-0.0035794717259705067,
0.05628620833158493,
-0.0015885707689449191,
-0.16952098906040192,
0.0243008304387331,
-0.02813638560473919,
0.041557736694812775,
-0.014746996574103832,
0.017902426421642303,
-0.014915824867784977,
-0.037679608911275864,
0.038221362978219986,
0.010043309070169926,
-0.047546617686748505,
-0.0602104514837265,
-0.045054174959659576,
-0.0046614836901426315,
0.003754254197701812,
-0.018427520990371704,
-0.010670588351786137,
0.07801505923271179,
0.04835449531674385,
0.005467098671942949,
-0.0491076298058033,
-0.015447847545146942,
-0.028902975842356682,
-0.08358745276927948,
-0.058686040341854095,
-0.02818036451935768,
-0.041443806141614914,
0.0027372899930924177,
-0.019242478534579277,
0.041053373366594315,
-0.059074755758047104,
0.03346686810255051,
-0.003871243679895997,
-0.001168150338344276,
-0.01943497359752655,
0.06554891169071198,
0.10755996406078339,
0.02586245723068714,
0.08929808437824249,
0.015538724139332771,
0.000525516108609736,
0.011193843558430672,
-0.010444454848766327,
-0.05658954009413719,
-0.04746631532907486,
-0.09771118313074112,
0.04122069478034973,
-0.06596770882606506,
-0.0004315903061069548,
0.0234100129455328,
0.0251521747559309,
-0.028146494179964066,
-0.03904953971505165,
0.06442702561616898,
0.005938282236456871,
0.08506868779659271,
0.03986213356256485,
-0.01442836131900549,
0.02563183195888996,
-0.017706317827105522,
-0.015340756624937057,
0.04679737612605095,
0.08452557027339935,
-0.2878080904483795,
-0.027240512892603874,
0.04904530569911003,
-0.045413825660943985,
0.04918719455599785,
-0.005575862713158131,
0.01749487966299057,
-0.009888455271720886,
-0.022371871396899223,
-0.014668776653707027,
-0.03598015382885933,
0.05007115378975868,
0.044930364936590195,
0.03474093973636627,
-0.028259608894586563,
0.0006775352521799505,
0.010275345295667648,
0.03807654231786728,
0.0034522046335041523,
-0.10677407681941986,
0.031793560832738876,
-0.034578148275613785,
0.09420567005872726,
-0.013301007449626923,
-0.009407862089574337,
0.04425548017024994,
0.000866740825586021,
0.005337373819202185,
-0.01007863786071539,
-0.021610954776406288,
-0.03352370858192444,
-0.02733788639307022,
0.05989556759595871,
-0.028976840898394585,
0.0067106373608112335,
0.050874125212430954,
-0.07137734442949295,
0.05779401212930679,
0.010070729069411755,
-0.013316263444721699,
-0.023494208231568336,
0.03769419714808464,
-0.03254212066531181,
-0.055746182799339294,
0.10604172199964523,
-0.0572483204305172,
-0.01796475052833557,
-0.04732581228017807,
0.007723182439804077,
0.0637015700340271,
-0.02801508456468582,
-0.04434659704566002,
0.01123017631471157,
0.03415221348404884,
-0.002154657384380698,
-0.01787591353058815,
-0.016608957201242447,
-0.006525721400976181,
0.06300487369298935,
0.000040787967009237036,
-0.05723857134580612,
-0.03787946328520775,
0.015734782442450523,
0.033466652035713196,
0.0704580619931221
] |
PMID:18766
|
Assay and characterization of serum-stimulated lipolytic activity in homogenates of human adipose tissue.
|
An assay of lipolytic activity in human adipose tissue is described, in which native homogenates of the adipose tissue yield the enzyme, as well as the triglyceride substrate, and the emulsifying phospholipids. The lipolytic activity in the presence of serum is characterized mainly as lipoprotein lipase activity by a pH-optimum of 8.0, by the fact that serum is necessary for full activity, and that it is inhibited by 1 M NaCl and by protamine. At serum concentrations of higher than 50% a marked inhibition of the lipolysis is observed. Noradrenaline, insulin, and heparin have no effect on the serum-stimulated lipolytic activity.
|
Assay and characterization of serum-stimulated lipolytic activity in homogenates of human adipose tissue. An assay of lipolytic activity in human adipose tissue is described, in which native homogenates of the adipose tissue yield the enzyme, as well as the triglyceride substrate, and the emulsifying phospholipids. The lipolytic activity in the presence of serum is characterized mainly as lipoprotein lipase activity by a pH-optimum of 8.0, by the fact that serum is necessary for full activity, and that it is inhibited by 1 M NaCl and by protamine. At serum concentrations of higher than 50% a marked inhibition of the lipolysis is observed. Noradrenaline, insulin, and heparin have no effect on the serum-stimulated lipolytic activity.
|
[
-0.021756762638688087,
0.0299840047955513,
0.01682247221469879,
0.011970998719334602,
-0.019405387341976166,
-0.026138871908187866,
0.08846387267112732,
0.0512942373752594,
-0.01877444237470627,
-0.03956586495041847,
0.020872248336672783,
-0.09926731139421463,
-0.031190786510705948,
0.03771485015749931,
-0.053416963666677475,
-0.031245000660419464,
0.02958758920431137,
0.007689930964261293,
-0.08265232294797897,
0.021306313574314117,
-0.00921422429382801,
0.022022895514965057,
-0.003640616312623024,
-0.01917368732392788,
0.0018669087439775467,
-0.008402230218052864,
-0.03428865224123001,
-0.03252078965306282,
-0.05391336604952812,
-0.17956696450710297,
0.05038394406437874,
-0.052818816155195236,
0.03304114192724228,
-0.01405165996402502,
0.05724307894706726,
0.01940443180501461,
-0.03240932896733284,
0.04476388916373253,
-0.016505775973200798,
0.03049027919769287,
0.033688463270664215,
0.014285307377576828,
-0.04589176923036575,
0.005697899032384157,
0.09765858948230743,
0.009055102244019508,
-0.01962571032345295,
0.044215794652700424,
0.019436253234744072,
0.040446653962135315,
0.052390359342098236,
0.03898895904421806,
0.04830614849925041,
0.05591246858239174,
0.06030764430761337,
-0.0394672155380249,
-0.04167424887418747,
0.04131650552153587,
-0.03020782768726349,
-0.009332037530839443,
-0.0289022047072649,
0.014728926122188568,
-0.13283579051494598,
0.06406882405281067,
0.009164150804281235,
-0.0037763877771794796,
0.005604904610663652,
-0.0029571009799838066,
0.006446646526455879,
0.0016812316607683897,
-0.052244339138269424,
0.07353848218917847,
0.005270411726087332,
-0.021194729954004288,
-0.0390813983976841,
0.03524664044380188,
0.011989421211183071,
0.02753596007823944,
-0.0002870252064894885,
-0.005865856539458036,
-0.01490470115095377,
-0.02915465272963047,
0.024123698472976685,
0.015161450952291489,
0.0146593963727355,
0.01750711165368557,
0.01225170586258173,
-0.0018813296919688582,
0.022590724751353264,
-0.009584658779203892,
-0.04478076100349426,
-0.05523780733346939,
-0.021575743332505226,
-0.0026648046914488077,
-0.07080206274986267,
0.038983460515737534,
0.030650660395622253,
-0.04760045185685158,
-0.01193755678832531,
0.3837023675441742,
0.013870783150196075,
0.01673894189298153,
-0.06466138362884521,
0.016316162422299385,
-0.006708636414259672,
-0.013293369673192501,
-0.06645060330629349,
-0.02667250856757164,
-0.04421916604042053,
0.049351319670677185,
0.05305706709623337,
0.06543595343828201,
-0.005438918713480234,
-0.07241696864366531,
0.008823945187032223,
-0.03802058473229408,
0.029246151447296143,
0.06231066957116127,
-0.006294626276940107,
0.03923831135034561,
-0.016647404059767723,
-0.00811840407550335,
0.009651608765125275,
-0.048114776611328125,
0.05045265704393387,
0.012856096029281616,
0.05888693407177925,
0.10898061096668243,
0.09140124171972275,
-0.0011802518274635077,
0.015103290788829327,
0.02544255740940571,
-0.035396166145801544,
-0.010002361610531807,
0.03044900670647621,
-0.0255148783326149,
0.04975968226790428,
-0.029267821460962296,
0.0397406667470932,
-0.03870783746242523,
-0.0013538089115172625,
-0.022181399166584015,
-0.055633336305618286,
-0.0697392076253891,
0.024552134796977043,
0.11986315995454788,
0.012311101891100407,
0.08101808279752731,
0.02038666233420372,
-0.024103017523884773,
-0.024609101936221123,
0.0053929961286485195,
-0.025643782690167427,
0.06221092864871025,
-0.027192050591111183,
-0.00136297638528049,
0.019628698006272316,
0.0020804996602237225,
-0.022864840924739838,
-0.06570453941822052,
-0.0991738811135292,
-0.04183722287416458,
0.004835204221308231,
0.03204187750816345,
0.01982569321990013,
0.0065680984407663345,
-0.006021780893206596,
0.0000792351711424999,
0.028455162420868874,
-0.02673611417412758,
0.009778935462236404,
-0.03338579460978508,
-0.026073530316352844,
0.003492877818644047,
-0.03606728836894035,
0.007047696970403194,
0.057722773402929306,
0.029351389035582542,
0.013576451689004898,
-0.005462778266519308,
0.038811326026916504,
-0.03774144873023033,
-0.025216195732355118,
-0.00031785419560037553,
0.017269089818000793,
-0.005888890940696001,
0.01073425356298685,
-0.006124239880591631,
0.026916489005088806,
0.05052606388926506,
-0.039038851857185364,
-0.007794306613504887,
-0.10199391096830368,
-0.029117723926901817,
-0.03525310382246971,
-0.0532972626388073,
0.006426398642361164,
0.022129548713564873,
-0.008659107610583305,
-0.01517532579600811,
0.08599361032247543,
0.03928409144282341,
0.037937283515930176,
-0.004741150885820389,
0.012476498261094093,
0.11596118658781052,
-0.055630095303058624,
0.02488538809120655,
0.08180172741413116,
0.01317409984767437,
-0.03378872945904732,
0.012369104661047459,
-0.06034621596336365,
-0.007782639004290104,
-0.03966202959418297,
0.035751622170209885,
-0.0002719680196605623,
-0.026518791913986206,
0.04525044560432434,
0.003606570651754737,
-0.012404419481754303,
-0.016041092574596405,
-0.023558741435408592,
-0.31432998180389404,
-0.014571288600564003,
0.07958655059337616,
-0.04452058672904968,
0.03928571566939354,
-0.038156233727931976,
0.024861468002200127,
-0.06392478942871094,
-0.037422824651002884,
-0.041908446699380875,
0.08494105935096741,
0.05521010234951973,
-0.02753610908985138,
-0.00392017001286149,
0.043063774704933167,
0.028454437851905823,
-0.01587762124836445,
-0.02334282547235489,
-0.010711649432778358,
-0.004800503142178059,
0.020132560282945633,
-0.051420025527477264,
0.004490026272833347,
0.00610767025500536,
0.14762113988399506,
-0.030779050663113594,
0.11598829925060272,
0.021314145997166634,
0.02032395638525486,
0.012453860603272915,
-0.009538602083921432,
0.07546902447938919,
-0.0190571378916502,
-0.12532970309257507,
-0.030219173058867455,
-0.04165096953511238,
0.010452291928231716,
-0.01172094140201807,
-0.01755637861788273,
0.017212387174367905,
-0.05976168438792229,
0.003915398847311735,
-0.04290507733821869,
0.0056376769207417965,
-0.05604598671197891,
-0.020409058779478073,
0.05941659212112427,
0.03605977073311806,
-0.007575617171823978,
0.012595655396580696,
0.057636700570583344,
-0.03954680636525154,
0.022708173841238022,
0.00010060948989121243,
0.011864010244607925,
-0.02316068299114704,
-0.04552675038576126,
0.02362152189016342,
-0.0687379464507103,
0.012867148034274578,
-0.0038792516570538282,
-0.0032481951639056206,
0.030241833999753,
0.047224778681993484,
0.006735103204846382,
-0.01827342063188553,
0.04996119812130928,
-0.0019969467539340258,
0.060228120535612106,
0.014171870425343513,
0.011821691878139973,
0.06968693435192108,
-0.027970200404524803,
-0.015449031256139278,
0.03203420341014862,
0.0032045335974544287,
0.0054759918712079525,
-0.06402339041233063,
-0.05076167359948158,
-0.0007324054604396224,
-0.04082600772380829,
-0.026746025308966637,
0.019938362762331963,
-0.01458704937249422,
-0.01088679488748312,
-0.022242899984121323,
0.03256099671125412,
-0.009731248021125793,
0.01586490124464035,
-0.008395190350711346,
0.01892838440835476,
0.002202063798904419,
-0.0446629673242569,
-0.0368344783782959,
-0.014949588105082512,
-0.027781987562775612,
-0.27824845910072327,
-0.019513368606567383,
0.01564420759677887,
0.0019349217182025313,
-0.0016120059881359339,
-0.05926136672496796,
0.012506645172834396,
0.0025627980940043926,
-0.07838790863752365,
0.05143293738365173,
-0.05297352001070976,
0.009430981241166592,
0.06110598146915436,
0.04695349186658859,
-0.014139327220618725,
-0.003826296189799905,
0.0116485096514225,
-0.04025614261627197,
-0.015129618346691132,
-0.0840260460972786,
0.010012442246079445,
-0.05767805501818657,
0.14678955078125,
-0.010703693144023418,
-0.013450756669044495,
0.03969746083021164,
0.006073350552469492,
-0.05444866418838501,
-0.048076778650283813,
0.012831661850214005,
-0.0006731973262503743,
0.015977274626493454,
0.05461442098021507,
0.01203944906592369,
0.00232568453066051,
0.12399054318666458,
0.03544240817427635,
-0.006846993695944548,
0.004399601370096207,
0.009757759980857372,
0.0469217412173748,
-0.023925989866256714,
-0.06279357522726059,
-0.03477822244167328,
-0.015398873947560787,
-0.05002746731042862,
-0.007159374188631773,
0.019848497584462166,
0.025560583919286728,
0.01448893267661333,
0.009267579764127731,
-0.05324171483516693,
0.011914064176380634,
0.02640516310930252,
0.06219794973731041,
-0.01701013557612896,
-0.046517904847860336,
0.00879934523254633,
-0.04158386215567589,
0.02714177779853344,
-0.0032363981008529663,
0.05200040712952614,
-0.053895168006420135,
0.0784609392285347,
0.045073069632053375
] |
PMID:18767
|
Oxygen affinity of duck blood determined by in vivo and in vitro technique.
|
Half saturation partial pressure of O2,P50, was determined in domestic Muscovy ducks (Cairina moschata) both by in vivo and in vitro techniques. For in vivo determination, blood samples were drawn from the anesthetized, artificially ventilated animals and analyzed both for O2 content, Co2, and O2 partial pressure, Po2. O2 capacity was detemined in arterial samples during hyperoxic ventilation (arterial Po2 about 180 torr). P50 was calculated from measurements in venous blood samples (O2 saturation near 50%). For in vitro determinations, Co2 was measured in blood samples equilibrated with Po2 close to P50. No significant difference was found between P50 values determined by both techniques. At 41 C and pH 7.50, P50 averaged 41.7 torr when analyzed by in vivo technique and 41.4 torr when determined in vitro. The variability between animals was less than 1 torr (SD) and could be explained by the experimental error. The partially discordant literature data on P50 of duck blood are reviewed and critically discussed.
|
Oxygen affinity of duck blood determined by in vivo and in vitro technique. Half saturation partial pressure of O2,P50, was determined in domestic Muscovy ducks (Cairina moschata) both by in vivo and in vitro techniques. For in vivo determination, blood samples were drawn from the anesthetized, artificially ventilated animals and analyzed both for O2 content, Co2, and O2 partial pressure, Po2. O2 capacity was detemined in arterial samples during hyperoxic ventilation (arterial Po2 about 180 torr). P50 was calculated from measurements in venous blood samples (O2 saturation near 50%). For in vitro determinations, Co2 was measured in blood samples equilibrated with Po2 close to P50. No significant difference was found between P50 values determined by both techniques. At 41 C and pH 7.50, P50 averaged 41.7 torr when analyzed by in vivo technique and 41.4 torr when determined in vitro. The variability between animals was less than 1 torr (SD) and could be explained by the experimental error. The partially discordant literature data on P50 of duck blood are reviewed and critically discussed.
|
[
-0.04737371951341629,
0.0016426000511273742,
-0.007975278422236443,
0.017166564241051674,
0.01724841259419918,
0.002643316751345992,
0.08545424789190292,
0.022473648190498352,
-0.04115647077560425,
0.05564705282449722,
-0.005376539658755064,
-0.15212348103523254,
-0.001495657954365015,
0.020258165895938873,
-0.064071886241436,
-0.036727242171764374,
-0.03213157132267952,
-0.01651695743203163,
-0.09378882497549057,
0.04866942763328552,
-0.039950501173734665,
-0.025851083919405937,
0.01941683143377304,
-0.03379177302122116,
0.06526503711938858,
0.03233017027378082,
-0.029394112527370453,
-0.010357585735619068,
-0.035999320447444916,
-0.1587238311767578,
-0.04998486489057541,
-0.054880790412425995,
-0.0024365682620555162,
-0.027019377797842026,
-0.0038360555190593004,
0.025167349725961685,
0.00914713367819786,
-0.01580752618610859,
-0.0010089784627780318,
0.062066081911325455,
0.03305217623710632,
-0.026562664657831192,
-0.02028924599289894,
0.02177710458636284,
0.03321780264377594,
0.014886877499520779,
-0.024621393531560898,
0.01677251234650612,
0.07690486311912537,
-0.02890140563249588,
-0.006192667409777641,
0.0034075379371643066,
-0.019916994497179985,
0.00297758006490767,
-0.008761946111917496,
-0.014185348525643349,
0.007731637917459011,
0.017878051847219467,
0.02793344482779503,
-0.03612500801682472,
0.018364226445555687,
0.06680649518966675,
-0.1335952877998352,
0.08010318130254745,
0.06519290804862976,
0.016570549458265305,
-0.017085643485188484,
-0.05293041840195656,
0.05475449562072754,
0.0006311000324785709,
-0.006089651025831699,
0.019576944410800934,
0.050274867564439774,
-0.006310977507382631,
-0.003369718324393034,
0.0015995941357687116,
-0.00904415175318718,
-0.008659381419420242,
0.006567802745848894,
0.013109127059578896,
-0.045528344810009,
0.0054781814105808735,
-0.033607881516218185,
0.012491755187511444,
-0.022331079468131065,
-0.016776591539382935,
0.06453695148229599,
-0.04625172168016434,
-0.015159182250499725,
0.0178570207208395,
-0.013419532217085361,
-0.03941986337304115,
-0.03231646865606308,
0.01420156192034483,
0.0120575912296772,
0.07436945289373398,
0.01824125088751316,
-0.01065459381788969,
0.013344645500183105,
0.3910508453845978,
0.016741150990128517,
-0.009433428756892681,
-0.07551543414592743,
-0.0204212237149477,
0.037131454795598984,
-0.02510770782828331,
0.017566055059432983,
0.021288488060235977,
0.006069097202271223,
0.04743146523833275,
0.043536752462387085,
0.0017589757917448878,
0.046451080590486526,
-0.02116761915385723,
-0.02408512309193611,
0.05322141945362091,
-0.013927584514021873,
0.06443258374929428,
-0.002760978415608406,
0.04386113956570625,
0.01138638611882925,
-0.057375241070985794,
0.03164222091436386,
-0.020828597247600555,
0.054623980075120926,
0.0019007778028026223,
0.02262168377637863,
0.057718224823474884,
0.019515547901391983,
0.034336332231760025,
0.034336525946855545,
0.0038933116011321545,
-0.01719401217997074,
-0.016580907627940178,
0.004681294783949852,
-0.008849743753671646,
0.02862013876438141,
0.011320758610963821,
0.008118430152535439,
-0.006477757822722197,
-0.00008847616845741868,
-0.019518379122018814,
-0.0040869382210075855,
-0.025126250460743904,
-0.07604026049375534,
0.1239069476723671,
0.06071114167571068,
0.033802926540374756,
-0.03759435936808586,
-0.07955866307020187,
-0.03699822351336479,
0.013839305378496647,
-0.01778753660619259,
0.015515088103711605,
0.029071375727653503,
0.036647845059633255,
-0.06101499870419502,
0.06398420035839081,
0.023774277418851852,
-0.039321381598711014,
-0.007408422417938709,
-0.0196251068264246,
-0.04371202737092972,
-0.003832964925095439,
0.02566980943083763,
0.008627502247691154,
-0.026580719277262688,
0.010772202163934708,
0.04650092124938965,
0.03754662349820137,
0.059650443494319916,
-0.026079850271344185,
-0.024423466995358467,
-0.03434429690241814,
0.004004997666925192,
0.0080838892608881,
-0.025919942185282707,
0.07181958854198456,
0.020728396251797676,
0.007640082389116287,
0.014750891365110874,
0.0237430389970541,
-0.04330333694815636,
0.031720247119665146,
0.026257870718836784,
-0.013742247596383095,
-0.04154787212610245,
-0.02330043539404869,
-0.013488507829606533,
0.08102238178253174,
-0.01834823191165924,
-0.056927863508462906,
-0.05072425678372383,
0.027601120993494987,
-0.014404197223484516,
-0.05429541692137718,
0.010064634494483471,
0.04849462956190109,
-0.011574581265449524,
-0.02816334366798401,
0.08263921737670898,
-0.021565333008766174,
0.024463070556521416,
0.04320504143834114,
0.060023970901966095,
0.06805732101202011,
0.06269403547048569,
-0.03161780163645744,
0.04541316628456116,
-0.017215602099895477,
-0.054397281259298325,
0.047805871814489365,
-0.0776229202747345,
0.017381861805915833,
-0.03955022990703583,
0.013068394735455513,
-0.006024072878062725,
-0.03170396015048027,
0.013440676033496857,
0.031741369515657425,
0.0572451613843441,
-0.1016775444149971,
-0.013335551135241985,
-0.3389831483364105,
-0.015571457333862782,
0.029802026227116585,
0.015122920274734497,
0.10332811623811722,
0.03481787070631981,
0.03570732846856117,
-0.017684856429696083,
0.0027639009058475494,
-0.014423787593841553,
-0.005756184924393892,
0.014372053556144238,
-0.002587274182587862,
-0.023004814982414246,
0.050097785890102386,
0.05159257724881172,
0.04511179402470589,
-0.05115242302417755,
0.013218410313129425,
0.05535338446497917,
0.06425485759973526,
0.007011024281382561,
-0.057856038212776184,
0.03850365802645683,
0.03332524001598358,
-0.006657459307461977,
0.1129208505153656,
-0.08351624011993408,
-0.014441397972404957,
-0.024080367758870125,
0.020422175526618958,
-0.0025298413820564747,
-0.016214018687605858,
-0.040353041142225266,
-0.02746184729039669,
0.010074435733258724,
-0.0649605318903923,
0.008719238452613354,
0.010181047953665257,
0.001169916009530425,
-0.02899530716240406,
0.03414162993431091,
0.020978469401597977,
-0.045267898589372635,
-0.04114827141165733,
-0.03060348518192768,
-0.02923787571489811,
-0.00825505517423153,
-0.08513157814741135,
-0.054742131382226944,
0.02004786767065525,
0.03167665749788284,
-0.026883330196142197,
-0.047907792031764984,
0.041323162615299225,
-0.0006292558391578496,
-0.02622261829674244,
0.022417323663830757,
-0.03816746547818184,
-0.07557961344718933,
0.020332500338554382,
-0.05351998656988144,
0.013416978530585766,
-0.01055180560797453,
-0.025483323261141777,
0.007013306021690369,
0.060418713837862015,
-0.025846797972917557,
0.0517987422645092,
0.028296522796154022,
0.04169359803199768,
0.06501401215791702,
-0.0012293808395043015,
0.04208553209900856,
0.06623376905918121,
0.05226943641901016,
0.013904398307204247,
-0.02989804558455944,
-0.0003676634805742651,
-0.009076211601495743,
0.08755839616060257,
-0.04754563421010971,
0.01604379154741764,
-0.008822550997138023,
-0.014796963892877102,
0.039616912603378296,
0.03364204615354538,
0.002362543251365423,
0.05287487059831619,
-0.03684977442026138,
-0.03488640487194061,
-0.0023982366546988487,
-0.03797011822462082,
-0.05712611600756645,
0.015451306477189064,
0.048028089106082916,
-0.2762073576450348,
-0.04987888038158417,
0.03366720303893089,
0.036075495183467865,
0.022236095741391182,
-0.010340908542275429,
0.016782792285084724,
-0.024846145883202553,
-0.028727056458592415,
0.007471755146980286,
-0.02414821647107601,
0.0021632083225995302,
0.03151192143559456,
0.05953454598784447,
-0.03300660103559494,
0.03595612943172455,
0.012760242447257042,
-0.0508698970079422,
-0.0003526515210978687,
-0.0448489673435688,
0.009948288090527058,
-0.12356774508953094,
0.11569736152887344,
-0.03861206769943237,
-0.0505395345389843,
0.0007017426542006433,
-0.017950018867850304,
0.030754350125789642,
0.016556663438677788,
-0.0026549892500042915,
0.02488379366695881,
-0.05165906250476837,
0.06756714731454849,
-0.08452440053224564,
0.026078252121806145,
0.04385930299758911,
-0.09988898783922195,
0.008985023945569992,
0.03577679395675659,
-0.039419952780008316,
0.027424676343798637,
-0.015420489944517612,
-0.07313505560159683,
-0.041122738271951675,
0.0904468521475792,
-0.017617134377360344,
0.008066629059612751,
-0.06051231175661087,
-0.00611071428284049,
0.002351772738620639,
-0.07751678675413132,
-0.04184318333864212,
0.04410024359822273,
-0.0005106832832098007,
0.04382501542568207,
-0.01245861779898405,
0.05339326709508896,
-0.027154644951224327,
0.004452953115105629,
-0.007595442701131105,
0.02024001255631447,
-0.008998685516417027,
-0.03778604418039322,
0.06375778466463089,
0.0257501769810915
] |
PMID:18787
|
Inhibition of secretin release and pancreatic bicarbonate secretion by somatostatin infusion in man.
|
Five healthy students were investigated on two different days with or without a constant infusion of somatostatin (500 microgram/h) into an arm vein a fluoroscopically placed Lagerlöf tube was used for the collection of gastric and duodenal juice. After 30-min basal period, 40 ml 100 mmol/l HCl was infused into the midpart of the duodenum over 5 min through a thin catheter attached to the tube. Plasma immunoreactive secretin was measured by radioimmunoassay employing 125I-labelled synthetic secretin, antibody against synthetic secretin, and standards prepared from pure natural porcine secretin. Secretin levels without somatostatin infusion were 4.6+/-0.7 pmol/l (mean+/-S.E.M.) basally and rose to a peak of 21.8+/-6.2 pmol/l after duodenal acidification (p less than 0.05) and with somatostatin infusion were 4.4+/-0.4 pmol/l basally and rose to a peak of 6.7+/-1.7 pmol/l (n.s.) after duodenal acidification. Pancreatic bicarbonate output increased from 8.0+/-2.5 mumol/min (mean+/-S.E.M.) to 283+/-44 mumol/min without somatostatin infusion (p less than 0.05) and from 6.7+/-2.1 mumol/min to 70+/-13 mumol/min somatostatin (p less than 0.05). This study shows that somatostatin (500 microgram/h can inhibit the release of secretin and the pancreatic bicarbonate secretion after duodenal acidification in man.
|
Inhibition of secretin release and pancreatic bicarbonate secretion by somatostatin infusion in man. Five healthy students were investigated on two different days with or without a constant infusion of somatostatin (500 microgram/h) into an arm vein a fluoroscopically placed Lagerlöf tube was used for the collection of gastric and duodenal juice. After 30-min basal period, 40 ml 100 mmol/l HCl was infused into the midpart of the duodenum over 5 min through a thin catheter attached to the tube. Plasma immunoreactive secretin was measured by radioimmunoassay employing 125I-labelled synthetic secretin, antibody against synthetic secretin, and standards prepared from pure natural porcine secretin. Secretin levels without somatostatin infusion were 4.6+/-0.7 pmol/l (mean+/-S.E.M.) basally and rose to a peak of 21.8+/-6.2 pmol/l after duodenal acidification (p less than 0.05) and with somatostatin infusion were 4.4+/-0.4 pmol/l basally and rose to a peak of 6.7+/-1.7 pmol/l (n.s.) after duodenal acidification. Pancreatic bicarbonate output increased from 8.0+/-2.5 mumol/min (mean+/-S.E.M.) to 283+/-44 mumol/min without somatostatin infusion (p less than 0.05) and from 6.7+/-2.1 mumol/min to 70+/-13 mumol/min somatostatin (p less than 0.05). This study shows that somatostatin (500 microgram/h can inhibit the release of secretin and the pancreatic bicarbonate secretion after duodenal acidification in man.
|
[
-0.007370123639702797,
-0.010747642256319523,
-0.018267927691340446,
-0.0011001534294337034,
-0.005734707694500685,
-0.022505976259708405,
0.02523963339626789,
0.03540363535284996,
-0.03185928240418434,
-0.041853029280900955,
-0.016213078051805496,
-0.08058281987905502,
0.030217811465263367,
0.04329831898212433,
0.030857518315315247,
-0.0060203964821994305,
0.034867942333221436,
-0.00463810795918107,
-0.07461338490247726,
-0.03574744984507561,
0.029980886727571487,
-0.0027951905503869057,
0.040747836232185364,
0.009549829177558422,
0.052850496023893356,
0.013461415655910969,
-0.020971888676285744,
-0.0391315259039402,
-0.036929432302713394,
-0.1924353837966919,
0.05382390692830086,
-0.02618018537759781,
0.011239051818847656,
-0.02797948382794857,
-0.002390307141467929,
-0.03832625225186348,
-0.023135583847761154,
0.030268466100096703,
-0.03172014281153679,
0.010532813146710396,
0.05073561519384384,
0.005204329267144203,
-0.005840757861733437,
-0.005801658611744642,
0.005269195418804884,
0.05135464668273926,
-0.07211893051862717,
0.008207708597183228,
0.07922502607107162,
0.05197647958993912,
0.03962203860282898,
0.03153258562088013,
-0.007466733921319246,
0.05870915949344635,
0.014370201155543327,
0.00801073107868433,
0.012417441233992577,
-0.011292407289147377,
-0.012089448980987072,
0.008794762194156647,
-0.00812962930649519,
0.054958030581474304,
-0.11934532225131989,
0.034324467182159424,
0.06666865944862366,
0.030293401330709457,
-0.0668550580739975,
-0.02571413293480873,
0.08030010759830475,
0.055242184549570084,
-0.045390862971544266,
0.06643903255462646,
-0.015777599066495895,
0.018358338624238968,
0.012221180833876133,
-0.025570686906576157,
0.012143129482865334,
0.007164120674133301,
0.033112719655036926,
0.037973787635564804,
0.007145976647734642,
0.019227925688028336,
0.035654764622449875,
-0.026912441477179527,
0.004122364800423384,
-0.02862429805099964,
0.03358433395624161,
0.0043105799704790115,
-0.03275342285633087,
-0.006756928749382496,
-0.023870423436164856,
-0.026568133383989334,
-0.04149308428168297,
0.001891919644549489,
-0.08378053456544876,
-0.015705447643995285,
0.011106905527412891,
-0.04222041368484497,
-0.037679415196180344,
0.38126447796821594,
0.0006991008995100856,
0.003133001970127225,
-0.055893708020448685,
-0.023338764905929565,
0.02417183108627796,
-0.010832048952579498,
-0.029128218069672585,
0.02456286922097206,
0.002699463628232479,
0.05287947133183479,
0.05998004600405693,
0.089365653693676,
0.041504666209220886,
-0.07031101733446121,
0.05922868475317955,
0.04253333806991577,
0.07716695219278336,
0.038226719945669174,
-0.006915094330906868,
0.11068108677864075,
-0.011713308282196522,
0.05774064362049103,
0.022303089499473572,
0.0006794888176955283,
0.024443579837679863,
0.004832015838474035,
0.00636700913310051,
0.06217579543590546,
-0.013616553507745266,
0.030862463638186455,
0.029047135263681412,
0.04566120356321335,
-0.024131910875439644,
0.018026698380708694,
-0.015172353014349937,
-0.07502347230911255,
0.0017296794103458524,
0.05823613330721855,
0.03849782794713974,
-0.009795233607292175,
-0.004501120187342167,
-0.06144658848643303,
0.0055222706869244576,
-0.06319271773099899,
-0.03570150211453438,
0.11776840686798096,
0.026368677616119385,
-0.02497127093374729,
-0.03766906261444092,
-0.026491684839129448,
0.03095114231109619,
0.019800420850515366,
-0.023295138031244278,
0.014548392035067081,
-0.026385817676782608,
0.030615046620368958,
-0.05093297362327576,
0.02894958108663559,
0.004219891503453255,
-0.021132376044988632,
-0.06917892396450043,
0.002360702957957983,
-0.03336627781391144,
0.016718661412596703,
0.01979629136621952,
-0.02850186824798584,
0.02355075068771839,
-0.022787239402532578,
0.018450574949383736,
-0.018295539543032646,
-0.02868504449725151,
0.02478669211268425,
-0.059151727706193924,
-0.0620926171541214,
0.0015786719741299748,
0.023614374920725822,
-0.00375584838911891,
-0.008344636298716068,
0.027263019233942032,
0.04186525568366051,
-0.02145417220890522,
0.020778624340891838,
-0.016688402742147446,
-0.006989073473960161,
0.00944722630083561,
0.000016238263924606144,
-0.05202142894268036,
0.02323916181921959,
0.005439721047878265,
0.11111071705818176,
-0.07603753358125687,
0.0159139446914196,
-0.16639257967472076,
0.014642187394201756,
-0.018842481076717377,
-0.06010908633470535,
-0.091576486825943,
-0.030716508626937866,
-0.030344270169734955,
0.03684157133102417,
0.05908944457769394,
0.031519584357738495,
0.005509763956069946,
0.0668092742562294,
0.06657224893569946,
0.022998694330453873,
-0.014966198243200779,
-0.06059199944138527,
0.04405205324292183,
0.001795073738321662,
-0.00815427303314209,
0.0541255921125412,
-0.05725366622209549,
0.045840244740247726,
0.013596112839877605,
0.06340112537145615,
-0.011753791011869907,
-0.040169890969991684,
-0.026650678366422653,
-0.030681980773806572,
0.014354763552546501,
-0.04264206066727638,
-0.05570729821920395,
-0.2946215569972992,
-0.0012949960073456168,
0.05387922748923302,
-0.02147030644118786,
0.1167568489909172,
-0.10147165507078171,
-0.00475132605060935,
-0.022711779922246933,
-0.017674867063760757,
0.011035995557904243,
0.028985317796468735,
0.05182861536741257,
-0.04009048268198967,
-0.025142131373286247,
0.008199555799365044,
-0.002685567596927285,
0.06854201853275299,
-0.04316132888197899,
0.04936915636062622,
-0.008710427209734917,
-0.030592668801546097,
0.031557440757751465,
0.041939184069633484,
-0.01262241043150425,
0.02335808239877224,
-0.014335504733026028,
0.12696640193462372,
-0.038897935301065445,
-0.0009247350972145796,
-0.02759629301726818,
-0.029233133420348167,
0.05291267856955528,
0.020346513018012047,
-0.042895007878541946,
-0.0013616784708574414,
-0.05596296116709709,
0.003397854743525386,
0.0018127602525055408,
-0.017802812159061432,
-0.02686765231192112,
-0.05912461876869202,
-0.022619720548391342,
0.0005240013124421239,
-0.0839882418513298,
-0.011410832405090332,
-0.03297821059823036,
-0.022407256066799164,
0.07150404155254364,
-0.001618938404135406,
0.01394078228622675,
0.04082038253545761,
-0.024967968463897705,
0.009815904311835766,
0.03451217710971832,
-0.03360680118203163,
-0.035485316067934036,
-0.0027920068241655827,
-0.041743163019418716,
-0.10489348322153091,
0.05496818572282791,
-0.04750974476337433,
-0.022131526842713356,
0.005662644747644663,
0.0005110338679514825,
-0.019264372065663338,
0.02427312359213829,
0.006077805068343878,
0.03835663944482803,
0.05542666092514992,
0.017275407910346985,
0.04816191643476486,
0.12075316160917282,
-0.05615266039967537,
0.03299499675631523,
0.08620918542146683,
0.029901400208473206,
0.032129574567079544,
-0.05705958977341652,
-0.060044631361961365,
-0.015896106138825417,
-0.024137161672115326,
-0.057842377573251724,
0.05638052895665169,
0.03569946810603142,
-0.04141192138195038,
0.047627225518226624,
0.030690904706716537,
-0.0031184658873826265,
-0.0035058618523180485,
0.037603456526994705,
0.01447489857673645,
0.041018176823854446,
-0.00780866015702486,
-0.04292615130543709,
-0.006875255145132542,
0.029672328382730484,
-0.2727319300174713,
0.03528919070959091,
0.014188256114721298,
-0.02225305326282978,
0.011030605994164944,
-0.022346707060933113,
0.043493349105119705,
-0.002480683848261833,
-0.056122057139873505,
0.03344045579433441,
-0.02956455573439598,
-0.0006821589195169508,
0.07478328794240952,
0.043213196098804474,
-0.011143493466079235,
-0.04140796884894371,
0.027650577947497368,
-0.01534136850386858,
0.003555574920028448,
-0.004038651939481497,
-0.022332245483994484,
-0.025381173938512802,
0.11344180256128311,
-0.00012479402357712388,
-0.010402888059616089,
-0.01059997733682394,
-0.014327695593237877,
0.027621112763881683,
0.011747811920940876,
0.027565505355596542,
0.007281106896698475,
0.03466949611902237,
-0.025225838646292686,
-0.03359317034482956,
0.03205767646431923,
0.00834486074745655,
0.024894172325730324,
0.007690883707255125,
0.01805022358894348,
0.06017501279711723,
-0.008687065914273262,
-0.05102836340665817,
-0.052153658121824265,
-0.05495583638548851,
0.04117349162697792,
-0.10409467667341232,
-0.008287826552987099,
-0.06685073673725128,
0.03654192015528679,
-0.0235644169151783,
-0.0275713000446558,
-0.07031984627246857,
0.07919783145189285,
0.029219098389148712,
-0.01831798627972603,
0.014078078791499138,
0.03881813585758209,
-0.05927018076181412,
-0.029667790979146957,
0.004830180201679468,
-0.0015581275802105665,
-0.05166930705308914,
-0.024629592895507812,
0.06589417904615402,
-0.02110566385090351
] |
PMID:18788
|
Gastro-oesophageal sphincter pressure, motility and acid clearing. A study of hiatus hernia patients and normal subjects and of the effect of a modified belsey MK IV repair on the results of the manometric and acid-clearing tests.
|
Basal gastro-oesophageal sphincter pressure was recorded in 68 patients with symptomatic, radiologically verified sliding hiatus hernia, and in 37 healthy subjects. The diameter of the probe was 2.5 mm, and the flow rate 0.5 ml/min. Mean sphincter pressure was lower in patients (6 mm Hg) than in normal subjects (15 mm Hg). In 32 per cent of the patients sphincter pressure was within normal range (8-24 mm Hg), whereas in 68 per cent it was lower than in the normal subjects. There seemed to be no relationship between sphincter pressure and severity of symptoms. Oesophageal acid clearing was investigated in 57 of the patients and in 26 of the normal subjects. Normal subjects clear the bolus of acid in an average of 10 swallows (range 4-16). In patients the incidence of prolonged clearing was greater, but the acid-clearing ability did not seem to be related to the degree of severity of the symptoms. A manometric study was made of 45 patients and an acid-clearing study made of 40 patients, before and 3 months after a modified Belsey MK IV repair for hiatal hernia. Mean postoperative sphincter pressure was higher (10 mm Hg) than the preoperative mean (6 mm Hg), but was still lower than the normal mean. Significant changes in acid-clearing ability could not be demonstrated. The results are inconclusive with regard to the importance of oesophageal motility disturbances for the symptomatology and acid-clearing ability. Thirty-seven out of 45 patients became free of symptoms, and the rest - except 1 (relapse of hernia) - improved.
|
Gastro-oesophageal sphincter pressure, motility and acid clearing. A study of hiatus hernia patients and normal subjects and of the effect of a modified belsey MK IV repair on the results of the manometric and acid-clearing tests. Basal gastro-oesophageal sphincter pressure was recorded in 68 patients with symptomatic, radiologically verified sliding hiatus hernia, and in 37 healthy subjects. The diameter of the probe was 2.5 mm, and the flow rate 0.5 ml/min. Mean sphincter pressure was lower in patients (6 mm Hg) than in normal subjects (15 mm Hg). In 32 per cent of the patients sphincter pressure was within normal range (8-24 mm Hg), whereas in 68 per cent it was lower than in the normal subjects. There seemed to be no relationship between sphincter pressure and severity of symptoms. Oesophageal acid clearing was investigated in 57 of the patients and in 26 of the normal subjects. Normal subjects clear the bolus of acid in an average of 10 swallows (range 4-16). In patients the incidence of prolonged clearing was greater, but the acid-clearing ability did not seem to be related to the degree of severity of the symptoms. A manometric study was made of 45 patients and an acid-clearing study made of 40 patients, before and 3 months after a modified Belsey MK IV repair for hiatal hernia. Mean postoperative sphincter pressure was higher (10 mm Hg) than the preoperative mean (6 mm Hg), but was still lower than the normal mean. Significant changes in acid-clearing ability could not be demonstrated. The results are inconclusive with regard to the importance of oesophageal motility disturbances for the symptomatology and acid-clearing ability. Thirty-seven out of 45 patients became free of symptoms, and the rest - except 1 (relapse of hernia) - improved.
|
[
0.032204050570726395,
-0.0029942328110337257,
0.018375448882579803,
0.05818532407283783,
-0.031943902373313904,
-0.0217917338013649,
0.05578596889972687,
0.021369755268096924,
-0.026545606553554535,
-0.056758880615234375,
-0.003295728238299489,
-0.1353527009487152,
-0.005989136639982462,
0.017789172008633614,
-0.04110342636704445,
0.0015689261490479112,
0.006328294053673744,
-0.013724797405302525,
-0.04271933436393738,
-0.011960218660533428,
-0.036476846784353256,
0.03477287292480469,
0.034766025841236115,
-0.022951966151595116,
0.02944435551762581,
-0.0027920755092054605,
0.007677202112972736,
-0.007884413003921509,
-0.06437468528747559,
-0.21246087551116943,
-0.028769060969352722,
-0.051521994173526764,
0.017535202205181122,
-0.05010576173663139,
-0.012745166197419167,
0.03545573353767395,
-0.0005094488733448088,
0.04934798926115036,
-0.016165491193532944,
0.03392242640256882,
0.03725019842386246,
0.0014780759811401367,
0.033323463052511215,
0.015693299472332,
0.023582153022289276,
0.032686177641153336,
-0.04920468479394913,
0.02455778233706951,
0.09570731222629547,
0.046147141605615616,
0.020787563174962997,
-0.034733060747385025,
-0.023441411554813385,
0.04104897752404213,
0.05929236859083176,
-0.04401255398988724,
0.032584235072135925,
0.007583318743854761,
-0.004016549792140722,
-0.032589785754680634,
-0.005428602918982506,
0.06376047432422638,
-0.1753406524658203,
0.026880992576479912,
0.04253002628684044,
-0.0359288826584816,
-0.00997220166027546,
-0.10403271019458771,
0.028852786868810654,
0.07505229115486145,
-0.03199191018939018,
0.04291115328669548,
-0.016754426062107086,
0.044282637536525726,
-0.013778156600892544,
-0.011580943129956722,
0.021366562694311142,
-0.027138719335198402,
-0.031240355223417282,
0.03974378481507301,
-0.005714082159101963,
-0.001450571813620627,
0.005292180459946394,
-0.04426731914281845,
0.0002572626108303666,
-0.05376777425408363,
0.03292106091976166,
0.006881697103381157,
-0.03078649379312992,
0.024751992896199226,
-0.024902667850255966,
-0.025366153568029404,
0.0016143519897013903,
-0.004564781207591295,
-0.04951629415154457,
-0.015131988562643528,
0.001230783062055707,
0.001691243494860828,
0.010372830554842949,
0.407061904668808,
-0.021654732525348663,
0.03269300237298012,
0.025475436821579933,
-0.024103723466396332,
0.00773300975561142,
0.00018822570564225316,
0.012593166902661324,
0.03829607367515564,
0.03981691226363182,
0.03812648355960846,
0.03875218704342842,
0.012189173139631748,
0.0405566468834877,
-0.008496999740600586,
-0.031968601047992706,
0.08298569917678833,
0.06482359021902084,
-0.013197943568229675,
0.0032263193279504776,
-0.029478784650564194,
-0.014384612441062927,
0.02039080485701561,
0.048470817506313324,
-0.036267563700675964,
0.01767626591026783,
0.019486743956804276,
0.00931412260979414,
0.08553770184516907,
0.040234532207250595,
-0.014204497449100018,
0.010046950541436672,
-0.04342411458492279,
-0.023400459438562393,
0.005368399433791637,
0.008344682864844799,
-0.058370884507894516,
0.0537872314453125,
0.016345564275979996,
-0.001462337328121066,
-0.012337405234575272,
-0.037082985043525696,
-0.09739141166210175,
-0.01374723482877016,
-0.07090240716934204,
-0.05871972069144249,
0.14634202420711517,
-0.058586250990629196,
0.013830202631652355,
-0.020510941743850708,
-0.004132885951548815,
0.05080625042319298,
0.02379268780350685,
-0.022337403148412704,
0.022144746035337448,
-0.028223589062690735,
-0.0014848902355879545,
-0.03252602741122246,
0.0456002801656723,
-0.054349444806575775,
0.03624381870031357,
-0.02533019706606865,
-0.03785841912031174,
-0.04730987176299095,
-0.013125995174050331,
0.05036472529172897,
-0.012716545723378658,
0.00430613337084651,
-0.021342793479561806,
-0.02168535441160202,
0.008063865825533867,
0.03859969601035118,
-0.0009540218161419034,
-0.0397014319896698,
-0.03306949511170387,
0.007794388569891453,
0.022464405745267868,
0.04294849559664726,
0.043011922389268875,
0.0023991665802896023,
0.005892130080610514,
0.016261259093880653,
0.05894587188959122,
0.0047178384847939014,
-0.0011987126199528575,
-0.027528982609510422,
0.003512246999889612,
-0.07372752577066422,
0.021648099645972252,
0.09050671011209488,
0.00998278521001339,
0.01095661986619234,
-0.05279364064335823,
-0.09913010895252228,
-0.0029881647787988186,
-0.028401225805282593,
-0.02016078680753708,
-0.0145005127415061,
-0.0067891813814640045,
-0.02086069993674755,
-0.001462179352529347,
0.07725036889314651,
0.021725326776504517,
-0.02653246559202671,
0.039044443517923355,
0.015594769269227982,
0.043192725628614426,
0.01574644446372986,
0.006682120729237795,
0.024476882070302963,
-0.01582837663590908,
-0.024284090846776962,
-0.008133454248309135,
-0.014913402497768402,
0.03151842951774597,
-0.0073087504133582115,
0.06656580418348312,
-0.014300931245088577,
-0.04127347469329834,
-0.06073693558573723,
0.027503279969096184,
0.08753596246242523,
0.011367205530405045,
-0.0025158291682600975,
-0.3065052330493927,
-0.002165325451642275,
0.024879245087504387,
-0.002574077108874917,
0.13754607737064362,
-0.04989275708794594,
0.04166457802057266,
0.006745888851583004,
0.043040115386247635,
-0.06621523201465607,
0.028864867985248566,
0.03474333882331848,
-0.07659492641687393,
0.01269446313381195,
0.008511127904057503,
0.06967570632696152,
0.06590601801872253,
-0.054637037217617035,
0.02111334167420864,
0.004965034779161215,
0.04542399197816849,
0.04279406741261482,
-0.010159685276448727,
-0.01592361368238926,
0.0283906739205122,
-0.028310276567935944,
0.11169742047786713,
-0.07329683750867844,
0.06709692627191544,
-0.03750620409846306,
0.01088686566799879,
-0.009724006056785583,
0.012699228711426258,
-0.05992989242076874,
-0.012501155957579613,
0.015604749321937561,
0.0047662281431257725,
0.006257445085793734,
-0.02291131019592285,
-0.01676604524254799,
-0.05908398702740669,
-0.032043106853961945,
-0.06521684676408768,
-0.053411126136779785,
-0.0330471470952034,
-0.013399872928857803,
-0.028475875034928322,
0.009464707225561142,
-0.023821532726287842,
0.006368797272443771,
0.0076075526885688305,
0.0018178772879764438,
-0.014561077579855919,
-0.02476736716926098,
0.0028096975293010473,
0.005293034948408604,
-0.024729924276471138,
-0.01157759502530098,
-0.0807761624455452,
-0.053462471812963486,
-0.07099808007478714,
-0.003903111442923546,
0.03912566974759102,
-0.000371661240933463,
0.018479466438293457,
-0.045948997139930725,
0.013691188767552376,
0.013480786234140396,
0.02373957633972168,
-0.0013080629287287593,
0.04014398530125618,
0.09394890815019608,
-0.06498648226261139,
0.011475231498479843,
0.05622250586748123,
0.04719929024577141,
0.009162772446870804,
0.027778584510087967,
-0.027901405468583107,
-0.04127075895667076,
0.01848161406815052,
-0.00683696661144495,
0.07174662500619888,
0.07161542028188705,
-0.02723318338394165,
0.0503101646900177,
-0.003178418381139636,
-0.060598332434892654,
0.028340350836515427,
-0.057789769023656845,
0.0445915088057518,
0.03279474005103111,
-0.01214583683758974,
-0.07025667279958725,
-0.004060850013047457,
0.009622320532798767,
-0.29007789492607117,
0.0265647042542696,
-0.01663396693766117,
0.010320844128727913,
-0.00940774381160736,
0.034812964498996735,
-0.0029542692936956882,
-0.018183501437306404,
0.007200631313025951,
0.0011311850976198912,
-0.05647457018494606,
0.016914786770939827,
0.015941789373755455,
0.0016050341073423624,
0.019774820655584335,
0.025995297357439995,
0.04865357279777527,
-0.019374869763851166,
0.046341150999069214,
-0.03993400186300278,
-0.015481382608413696,
-0.04984046891331673,
0.10880013555288315,
0.032482996582984924,
0.03631037473678589,
0.023059546947479248,
0.017316075041890144,
0.0395272858440876,
0.03798304870724678,
0.0020201706793159246,
0.012742625549435616,
0.026525823399424553,
0.03693613409996033,
0.003661438589915633,
0.030635284259915352,
-0.02139205113053322,
-0.04146694764494896,
0.006464427802711725,
0.003926727920770645,
-0.01690981164574623,
0.05351390317082405,
-0.01861235313117504,
-0.01177575346082449,
-0.07094734907150269,
0.05551520735025406,
-0.09745963662862778,
-0.03666798025369644,
-0.06513064354658127,
-0.017458956688642502,
-0.03817922994494438,
-0.007918008603155613,
-0.03275667130947113,
0.0657431036233902,
0.05367527902126312,
-0.004110004752874374,
0.04643787443637848,
0.011068412102758884,
-0.015711240470409393,
0.04594732075929642,
-0.018609756603837013,
0.016383130103349686,
-0.0020205865148454905,
-0.018920235335826874,
0.03717822581529617,
0.019423525780439377
] |
PMID:18789
|
Enzymatic Determination of bile acids. the NADP-specific 7alpha-hydroxysteroid dehydrogenase from P. testosteroni (ATCC 11996).
|
By use of the specific substrates, 7alpha-hydroxy-5beta-cholanic acid and 3alpha, 7alpha, 12alpha-trihydroxy-3,12-diacetyl-5beta-cholanic acid methyl ester, crude extracts of P. testosteroni grown on a steroid-containing medium have been shown to exhibit 7alpha-hydroxysteroid: NADP-oxidoreductase activity. The enzyme is highly specific for NADP. Both free and conjugated 7alpha-hydroxy bile acids can act as substrates, but those of low polarity (few hydroxyl groups) seem to be preferred, judging from initial reaction velocity studies. Optimal conditions appears to be at pH 8.5-9.5 and at 25 degress C. Free SH-groups are essential for maximum catalytic activity, since the enzyme is inhibited by SH-reacting substances such as p-chloromercuribenzoate and monoiodoacetic acid. Also the ketone-trapping agents hydrazine hydrate and semicarbazide act as inhibitors. Upon dilution, the storage stability is severely reduced, but this effect may be counteracted by the addition of glycerol at concentration of 20% or more. By gel filtration experiments on Sephadex G-100, the molecular weight was estimated to about 80,000.
|
Enzymatic Determination of bile acids. the NADP-specific 7alpha-hydroxysteroid dehydrogenase from P. testosteroni (ATCC 11996). By use of the specific substrates, 7alpha-hydroxy-5beta-cholanic acid and 3alpha, 7alpha, 12alpha-trihydroxy-3,12-diacetyl-5beta-cholanic acid methyl ester, crude extracts of P. testosteroni grown on a steroid-containing medium have been shown to exhibit 7alpha-hydroxysteroid: NADP-oxidoreductase activity. The enzyme is highly specific for NADP. Both free and conjugated 7alpha-hydroxy bile acids can act as substrates, but those of low polarity (few hydroxyl groups) seem to be preferred, judging from initial reaction velocity studies. Optimal conditions appears to be at pH 8.5-9.5 and at 25 degress C. Free SH-groups are essential for maximum catalytic activity, since the enzyme is inhibited by SH-reacting substances such as p-chloromercuribenzoate and monoiodoacetic acid. Also the ketone-trapping agents hydrazine hydrate and semicarbazide act as inhibitors. Upon dilution, the storage stability is severely reduced, but this effect may be counteracted by the addition of glycerol at concentration of 20% or more. By gel filtration experiments on Sephadex G-100, the molecular weight was estimated to about 80,000.
|
[
-0.0611475333571434,
0.031683165580034256,
-0.01232332456856966,
0.03631077706813812,
-0.014743139035999775,
0.005587898660451174,
0.021316679194569588,
0.034145791083574295,
-0.0481603629887104,
-0.06622261554002762,
0.041635915637016296,
-0.10195834189653397,
0.07327181100845337,
-0.03934015706181526,
-0.05786716938018799,
-0.06473858654499054,
0.06523732095956802,
0.0004253357765264809,
0.02661445364356041,
0.030275311321020126,
-0.003649077145382762,
0.05504722148180008,
-0.0013321232981979847,
-0.029895972460508347,
0.005639845505356789,
0.01647263765335083,
0.030740398913621902,
-0.003366760676726699,
-0.08461549133062363,
-0.17317990958690643,
0.001744678127579391,
0.0068879565224051476,
0.056927647441625595,
-0.10713428258895874,
0.008421494625508785,
0.04896826297044754,
0.018173525109887123,
-0.023366235196590424,
-0.005016656592488289,
-0.00984027236700058,
0.046772301197052,
0.009437977336347103,
0.009679813869297504,
0.05774753540754318,
-0.0014768074033781886,
0.050122398883104324,
-0.011707350611686707,
0.0036869512405246496,
0.062115248292684555,
-0.021797969937324524,
0.032976992428302765,
0.0058906893245875835,
0.007349109742790461,
-0.02169814333319664,
-0.029222046956419945,
-0.0704604983329773,
0.04375152289867401,
0.06985104084014893,
-0.04140520840883255,
0.005884332116693258,
0.003086581826210022,
-0.010588785633444786,
-0.14835597574710846,
0.005478487815707922,
0.03584764897823334,
0.04901869595050812,
-0.014286233112215996,
-0.06373166292905807,
0.023829171434044838,
0.031292788684368134,
-0.03390379250049591,
0.030321838334202766,
-0.023129085078835487,
0.021448342129588127,
0.0394546315073967,
0.007998380810022354,
-0.026995336636900902,
-0.02189035154879093,
-0.024527866393327713,
-0.002945746760815382,
0.011633123271167278,
0.047207269817590714,
-0.029678793624043465,
0.03197861835360527,
0.001365938107483089,
0.046607453376054764,
0.04212440922856331,
0.008398083969950676,
0.06289159506559372,
-0.0039488221518695354,
0.018864594399929047,
-0.022651098668575287,
-0.03323733061552048,
-0.010135005228221416,
-0.08892547339200974,
0.06068313494324684,
0.004468685016036034,
0.019051235169172287,
-0.012275991030037403,
0.3736220598220825,
-0.034019116312265396,
0.000643520150333643,
-0.07384224981069565,
-0.053068481385707855,
-0.012678291648626328,
-0.038021404296159744,
-0.008171283639967442,
-0.004939438309520483,
0.02010147087275982,
0.0004791381361428648,
0.00948511902242899,
0.002352423733100295,
0.03919418901205063,
-0.10664628446102142,
-0.07638656347990036,
-0.024723289534449577,
0.012521255761384964,
-0.0005689100362360477,
-0.05233253538608551,
-0.027441106736660004,
-0.007040917407721281,
0.02703661471605301,
0.04914600029587746,
0.0048681218177080154,
0.08827527612447739,
0.03527708351612091,
-0.0032892313320189714,
0.08510524779558182,
0.07236813753843307,
0.04346957057714462,
-0.00199810229241848,
0.03533497825264931,
-0.01866045966744423,
-0.01902150735259056,
0.02128436788916588,
0.003446626476943493,
0.07441245764493942,
0.0066985152661800385,
0.009470820426940918,
-0.01880357228219509,
-0.05306975543498993,
-0.032961852848529816,
-0.041579246520996094,
-0.026673289015889168,
-0.06124340370297432,
0.09905515611171722,
0.007776537910103798,
-0.009853111580014229,
-0.03313019126653671,
-0.0025040465407073498,
-0.012753547169268131,
0.030631767585873604,
-0.034136537462472916,
0.04745308309793472,
-0.01930975541472435,
-0.009595490992069244,
-0.008953300304710865,
-0.04540001228451729,
0.004614448640495539,
-0.017317134886980057,
-0.07155713438987732,
0.01634436845779419,
0.01184586901217699,
0.08415676653385162,
0.03303596004843712,
-0.014206891879439354,
-0.04574109986424446,
0.022586332634091377,
0.005308930762112141,
0.013043022714555264,
0.012483164668083191,
0.008626462891697884,
-0.024355968460440636,
-0.030767293646931648,
-0.048638954758644104,
-0.031217563897371292,
0.05246862396597862,
0.10028687864542007,
0.01976657472550869,
-0.011304629035294056,
0.013669587671756744,
-0.039585039019584656,
-0.02165236324071884,
-0.028786584734916687,
0.004261123016476631,
-0.018509309738874435,
-0.04243582487106323,
0.01827976107597351,
-0.006746895145624876,
0.038017891347408295,
-0.04046561196446419,
-0.008023221045732498,
-0.09932340681552887,
-0.02742297574877739,
-0.050638072192668915,
-0.048341549932956696,
-0.0576377809047699,
0.03566146269440651,
0.04346160218119621,
0.003659467911347747,
0.08565982431173325,
0.05088840797543526,
0.03836089372634888,
0.06172701343894005,
0.001963344868272543,
0.0421564020216465,
0.007093710359185934,
0.014353795908391476,
0.015642009675502777,
0.04902578517794609,
-0.04463290795683861,
0.018773701041936874,
-0.0333838015794754,
0.04540996626019478,
-0.06716075539588928,
0.050915542989969254,
-0.010866728611290455,
-0.02414744347333908,
0.04210454598069191,
0.0031133827287703753,
0.043448492884635925,
-0.06417036056518555,
0.0021988842636346817,
-0.31783902645111084,
-0.06333761662244797,
-0.014548307284712791,
0.011041587218642235,
0.05081962049007416,
-0.05743948370218277,
-0.039470426738262177,
-0.01669684611260891,
-0.034592535346746445,
-0.031328994780778885,
0.06648790091276169,
0.048016343265771866,
-0.06660090386867523,
-0.05116530507802963,
-0.05105534568428993,
0.029582837596535683,
0.018451089039444923,
-0.048169758170843124,
0.027582630515098572,
0.003678352804854512,
0.05637597665190697,
-0.056628819555044174,
0.018674103543162346,
0.013974150642752647,
0.07305994629859924,
-0.004119474440813065,
0.0797376036643982,
0.0011534180957823992,
-0.0025232580956071615,
0.004985353443771601,
0.06241215020418167,
0.019184403121471405,
-0.04369514435529709,
-0.0858476310968399,
0.013071571476757526,
-0.07608259469270706,
-0.0033037911634892225,
-0.11478786170482635,
0.017045676708221436,
0.004785377532243729,
0.04743456095457077,
0.030362483114004135,
-0.04039972648024559,
-0.0911257341504097,
-0.038252197206020355,
-0.04223887249827385,
0.026866527274250984,
0.04608859121799469,
0.025784309953451157,
-0.008631912991404533,
-0.004145028535276651,
0.019283751025795937,
-0.030903028324246407,
-0.07267814874649048,
-0.0065220072865486145,
-0.018121516332030296,
0.005921998526901007,
-0.048341814428567886,
-0.08326741307973862,
0.046987250447273254,
-0.01874321699142456,
0.041351497173309326,
-0.0021338320802897215,
0.028725046664476395,
0.0200574342161417,
-0.008797031827270985,
-0.01414436474442482,
-0.05420931428670883,
0.04403483867645264,
0.013273819349706173,
0.029535667970776558,
0.08103016763925552,
-0.01860353909432888,
0.02321004867553711,
0.01929417997598648,
0.024309232831001282,
-0.005009194370359182,
-0.06149240583181381,
-0.02227865904569626,
0.02713702991604805,
-0.0019469668623059988,
-0.04337470605969429,
0.046611178666353226,
-0.01699823886156082,
0.011390799656510353,
0.0468314103782177,
0.03463860973715782,
-0.07029498368501663,
0.05044521018862724,
0.06253324449062347,
0.06361885368824005,
0.011247175745666027,
-0.004648732021450996,
-0.0003875411639455706,
0.023754199966788292,
-0.0035645144525915384,
-0.2510766386985779,
-0.0006015194812789559,
-0.017817143350839615,
-0.0014731122646480799,
0.023431451991200447,
0.07804694771766663,
-0.0174450371414423,
-0.01240757666528225,
-0.0666099414229393,
0.012514014728367329,
0.04957469180226326,
0.043399300426244736,
0.047579076141119,
0.06845328956842422,
0.026865802705287933,
-0.007945210672914982,
0.02962212637066841,
0.014047627337276936,
0.006739674601703882,
-0.059541016817092896,
0.03353503718972206,
-0.05890242010354996,
0.0952577292919159,
0.03795929625630379,
-0.04661140590906143,
0.049807608127593994,
0.0072737568989396095,
0.023919083178043365,
-0.016008488833904266,
-0.0038159401156008244,
-0.02969040721654892,
0.03408709168434143,
0.07766460627317429,
0.006422498729079962,
0.01753273233771324,
0.06444739550352097,
-0.01224767230451107,
-0.006091921124607325,
-0.008938593789935112,
0.06523991376161575,
0.008139831013977528,
-0.021067073568701744,
-0.10154858231544495,
0.031097397208213806,
0.052079230546951294,
-0.09701704233884811,
-0.05635469779372215,
-0.020637206733226776,
0.010133570060133934,
0.01610269397497177,
0.006414149422198534,
-0.0049865152686834335,
0.022111976519227028,
0.052292350679636,
-0.003965390846133232,
0.014447461813688278,
0.01268432755023241,
-0.011356682516634464,
-0.03304393216967583,
-0.005146144889295101,
0.0027452302165329456,
0.01490118820220232,
-0.03189128264784813,
0.13136722147464752,
0.00777016393840313
] |
PMID:18790
|
Quantitative determination of serum bile acids using a 7alpha-hydroxysteroid dehydrogenase.
|
A crude 7alpha-hydroxysteroid dehydrogenase isolate from P. testosteroni requires NADP specifically and provides a simple and specific means for the determination of 7alpha-hydroxy bile acids and their conjugates. A specific and reliable enzymatic-fluorimetric method for the quantitative measurement of the primary bile acids in serum is described. Overall recovery from normal and jaundiced serum averaged 85%. The mean concentration of serum 7alpha-hydroxy bile acids in fasting, healthy subjects was 2.5 mumoles/1 (S.D. 1.45 AND RANGE 1.2-6.3), averaging 73% (S.D. 11.5) of the total, measured as the 3alpha-hydroxy bile acid concentration. In parenchymatous liver disease and cholestatic hepatobiliary disorders, the proportion of 7alpha-hydroxy bile acids was significantly increased, mean 92.5% (S.D. 6.6)(p less than 0.001).
|
Quantitative determination of serum bile acids using a 7alpha-hydroxysteroid dehydrogenase. A crude 7alpha-hydroxysteroid dehydrogenase isolate from P. testosteroni requires NADP specifically and provides a simple and specific means for the determination of 7alpha-hydroxy bile acids and their conjugates. A specific and reliable enzymatic-fluorimetric method for the quantitative measurement of the primary bile acids in serum is described. Overall recovery from normal and jaundiced serum averaged 85%. The mean concentration of serum 7alpha-hydroxy bile acids in fasting, healthy subjects was 2.5 mumoles/1 (S.D. 1.45 AND RANGE 1.2-6.3), averaging 73% (S.D. 11.5) of the total, measured as the 3alpha-hydroxy bile acid concentration. In parenchymatous liver disease and cholestatic hepatobiliary disorders, the proportion of 7alpha-hydroxy bile acids was significantly increased, mean 92.5% (S.D. 6.6)(p less than 0.001).
|
[
-0.028827780857682228,
0.005185018293559551,
-0.0034082678612321615,
0.012913631275296211,
-0.01750532165169716,
0.004638293758034706,
0.043181661516427994,
0.0368259958922863,
-0.005332260858267546,
-0.05708136782050133,
0.0570930652320385,
-0.1477065533399582,
0.07996123284101486,
-0.04909203201532364,
-0.04899896681308746,
-0.05684911832213402,
0.09729230403900146,
-0.04433998838067055,
-0.01395723782479763,
0.05488785356283188,
-0.03744347393512726,
0.039858222007751465,
-0.014570260420441628,
-0.060663335025310516,
0.012268118560314178,
0.04622368887066841,
0.027301760390400887,
0.00009253793541574851,
-0.08699271082878113,
-0.15964213013648987,
0.02657044678926468,
0.0033565110061317682,
0.07227867841720581,
-0.07827792316675186,
-0.00879110861569643,
0.030613724142313004,
-0.002895559649914503,
-0.022750509902834892,
0.06309514492750168,
0.013530060648918152,
0.03882310166954994,
0.036078743636608124,
-0.005612364504486322,
0.07099083811044693,
-0.029517341405153275,
0.004132420755922794,
-0.018297484144568443,
0.015750164166092873,
0.08155827969312668,
0.0165450070053339,
0.03902922198176384,
0.0045046028681099415,
0.006861719768494368,
0.031483810395002365,
-0.02127349004149437,
-0.05920629948377609,
0.025428857654333115,
0.039424292743206024,
-0.029044395312666893,
-0.00475997943431139,
-0.012191716581583023,
-0.010887783020734787,
-0.12205619364976883,
0.02635929547250271,
0.0024864424485713243,
0.003873740090057254,
-0.019850052893161774,
-0.06890880316495895,
0.01726178638637066,
0.04748246446251869,
-0.05387044697999954,
0.009344153106212616,
-0.027339084073901176,
0.04337495192885399,
0.022784017026424408,
-0.004904445260763168,
-0.011339949443936348,
-0.04476386681199074,
-0.03434234485030174,
0.018783576786518097,
-0.023519620299339294,
0.04115717113018036,
-0.031863849610090256,
0.038039762526750565,
-0.007225946057587862,
0.011067375540733337,
0.02944226562976837,
0.006002312991768122,
0.06366655975580215,
-0.02639269456267357,
0.012938165105879307,
-0.043108128011226654,
-0.05095791444182396,
0.007773085962980986,
-0.05435667186975479,
0.028807014226913452,
-0.005523321218788624,
0.0076096681877970695,
0.04151604324579239,
0.36600998044013977,
-0.013391160406172276,
-0.02486414834856987,
-0.056793954223394394,
-0.05160682275891304,
-0.017668871209025383,
-0.017254097387194633,
-0.025579890236258507,
-0.04681934416294098,
0.0032786570955067873,
0.00021099860896356404,
0.011272323317825794,
-0.037092212587594986,
0.08864486217498779,
-0.10138588398694992,
-0.05230836570262909,
0.01209461409598589,
0.020173370838165283,
-0.028103984892368317,
-0.019945768639445305,
-0.019360454753041267,
0.008066980168223381,
0.03451060131192207,
0.08320920914411545,
-0.021628661081194878,
0.07254624366760254,
0.04392014816403389,
0.008209023624658585,
0.08395805954933167,
0.06347131729125977,
0.08434280753135681,
-0.028453171253204346,
0.04032790660858154,
-0.043302055448293686,
-0.033893074840307236,
0.02546524628996849,
0.009595107287168503,
0.09510506689548492,
-0.0170595683157444,
0.03413369506597519,
0.06172650679945946,
-0.01165451668202877,
-0.059242840856313705,
-0.05685485899448395,
-0.019510533660650253,
-0.039989788085222244,
0.13260339200496674,
-0.005440142937004566,
-0.010145500302314758,
-0.011248019523918629,
-0.0651182010769844,
0.04385717958211899,
0.016476672142744064,
-0.04736049845814705,
0.03377330303192139,
-0.004877936094999313,
-0.010266887955367565,
-0.006805610843002796,
-0.008914410136640072,
-0.006758319679647684,
-0.037364281713962555,
-0.08205725997686386,
-0.014487643726170063,
0.003911897074431181,
0.06183866411447525,
0.030846739187836647,
-0.026367681100964546,
-0.033015888184309006,
0.007135046645998955,
-0.006488169077783823,
-0.001557007897645235,
0.05133775994181633,
-0.018207909539341927,
-0.06318265199661255,
-0.0013083708472549915,
-0.005685142707079649,
-0.043006084859371185,
0.03378092125058174,
0.10388407111167908,
0.01979237236082554,
-0.020527852699160576,
0.03230874985456467,
0.003933892119675875,
-0.0333387516438961,
-0.0018664572853595018,
0.0009004499879665673,
-0.03437517210841179,
-0.03278234601020813,
0.032130852341651917,
-0.020930342376232147,
0.057366132736206055,
-0.03370494022965431,
0.02767554484307766,
-0.10970404744148254,
-0.05243586003780365,
-0.002358723431825638,
-0.023601267486810684,
-0.07198954373598099,
0.0035176773089915514,
0.052772168070077896,
-0.006859298329800367,
0.08657301217317581,
-0.0018574431305751204,
0.03655562177300453,
0.073575459420681,
0.02494857646524906,
0.03880443423986435,
0.02405065856873989,
0.0007606694125570357,
0.03129763901233673,
0.07586587965488434,
-0.035438086837530136,
-0.007598245050758123,
-0.0401211753487587,
0.032775819301605225,
-0.06616535037755966,
0.05744151398539543,
0.016196008771657944,
-0.04832806810736656,
0.023381635546684265,
0.006428319029510021,
0.06411336362361908,
-0.05247369036078453,
0.015726808458566666,
-0.28745508193969727,
-0.062408603727817535,
0.01719806343317032,
0.009858488105237484,
0.046105191111564636,
-0.059512633830308914,
-0.03196876123547554,
-0.022515490651130676,
0.028481628745794296,
-0.009985198266804218,
0.05577240139245987,
0.037503283470869064,
-0.07623033225536346,
-0.04962978512048721,
-0.03633318841457367,
0.03808603808283806,
0.04882917180657387,
-0.020803190767765045,
0.03352435678243637,
0.00026370430714450777,
0.0430636890232563,
-0.027854857966303825,
0.023853342980146408,
0.04214920848608017,
0.07130970805883408,
-0.03729753941297531,
0.09998775273561478,
-0.05943579971790314,
-0.024403991177678108,
-0.011232198216021061,
0.0400044247508049,
0.029215048998594284,
-0.04745886102318764,
-0.04195086285471916,
0.04366280511021614,
-0.06316599249839783,
-0.03963347151875496,
-0.09637369215488434,
-0.021876951679587364,
-0.010586417280137539,
0.009862557053565979,
0.015899742022156715,
-0.011551707051694393,
-0.06295684725046158,
-0.060474641621112823,
-0.024649104103446007,
0.011226254515349865,
0.005151221062988043,
0.006801331881433725,
-0.0021614234428852797,
0.004384891130030155,
-0.026681475341320038,
-0.038750432431697845,
-0.05136091634631157,
-0.03171706199645996,
-0.030459795147180557,
0.015041914768517017,
-0.061922330409288406,
-0.09054242819547653,
0.013341564685106277,
-0.033317726105451584,
0.030843885615468025,
0.020901275798678398,
-0.004166814498603344,
0.003995147068053484,
-0.022375378757715225,
0.016941383481025696,
-0.049203019589185715,
0.043505046516656876,
0.029254920780658722,
0.042729444801807404,
0.10543549805879593,
-0.02844715118408203,
0.022538119927048683,
-0.0020167145412415266,
0.048236455768346786,
0.013243820518255234,
-0.057653117924928665,
-0.021069331094622612,
-0.006354642566293478,
0.03394439443945885,
-0.08069353550672531,
0.025478098541498184,
-0.005225893575698137,
0.03914201259613037,
0.048443250358104706,
0.06486675888299942,
-0.028881721198558807,
0.023509489372372627,
0.05493474379181862,
0.023736145347356796,
0.02728160284459591,
-0.007661976385861635,
-0.02241712249815464,
-0.056337036192417145,
0.0017060785321518779,
-0.2589801251888275,
0.009486624971032143,
-0.030524006113409996,
-0.0173941757529974,
0.05846383050084114,
0.04046408832073212,
0.005630308762192726,
-0.017185095697641373,
-0.07318378239870071,
-0.011657211929559708,
0.05934414640069008,
0.04808569699525833,
0.04248287156224251,
0.02458113059401512,
0.0156883392482996,
-0.02418404631316662,
0.03318772464990616,
0.020902395248413086,
0.0024664998054504395,
-0.05288495495915413,
0.020789314061403275,
-0.08028143644332886,
0.09248265624046326,
0.033055707812309265,
-0.025209199637174606,
0.032110560685396194,
0.01873485930263996,
0.02598576806485653,
-0.00941814947873354,
-0.01118455920368433,
-0.017984114587306976,
0.028476865962147713,
0.07397712767124176,
0.0020351209677755833,
0.003004352329298854,
0.05246402323246002,
-0.0005407542339526117,
-0.014600376598536968,
0.00789156649261713,
0.07038246840238571,
0.05156721547245979,
-0.011519426479935646,
-0.06822985410690308,
-0.009803547523915768,
0.045102380216121674,
-0.08309072256088257,
-0.028852295130491257,
-0.0258528720587492,
0.006683018058538437,
0.013508318923413754,
-0.0033492192160338163,
0.0051284972578287125,
0.012359618209302425,
0.06157853081822395,
-0.010474000126123428,
0.03073233552277088,
0.008327229879796505,
0.02650037594139576,
-0.019491741433739662,
-0.03454197198152542,
0.005870717111974955,
0.00987309217453003,
-0.033531274646520615,
0.1401703953742981,
0.0032516068313270807
] |
PMID:18791
|
Effect of cimetidine on pentagastrin-stimulated gastric secretion in healthy man.
|
Two series of experiments were carried out in three healthy volunteers. In one series increasing doses of cimetidine were tested on a constant background stimulation with 0.15 microgram-kg-1h-1 of pentagastrin. In the other series of experiments increasing doses of pentagastrin were given either alone or in combination with a fixed dose of cimetidine (1.2 mg-kg-1h-1). Pentagastrin and cimetidine were given as continuous intravenous infusions, the various doses tested on separate days. The inhibition of acid output in response to pentagastrin stimulation increased almost linearly with the log dose of cimetidine. Fifty per cent inhibition of the response to 0.15 microgram-kg-1h-1 of pentagastrin was achieved by about 0.6 mg-kg-1h-1 of cimetidine. 2.4 mg-kg-1h-1 of cimetidine gave on average 90% inhibition. The inhibition caused by cimetidine decreased to a minimum of 57% and could not be overcome by increasing the doses of pentagastrin. The results suggest a mixture of competitive and noncompetitive interaction. The effect of cimetidine on pepsin secretion was less regular. Generally, pepsin output was less inhibited than acid output.
|
Effect of cimetidine on pentagastrin-stimulated gastric secretion in healthy man. Two series of experiments were carried out in three healthy volunteers. In one series increasing doses of cimetidine were tested on a constant background stimulation with 0.15 microgram-kg-1h-1 of pentagastrin. In the other series of experiments increasing doses of pentagastrin were given either alone or in combination with a fixed dose of cimetidine (1.2 mg-kg-1h-1). Pentagastrin and cimetidine were given as continuous intravenous infusions, the various doses tested on separate days. The inhibition of acid output in response to pentagastrin stimulation increased almost linearly with the log dose of cimetidine. Fifty per cent inhibition of the response to 0.15 microgram-kg-1h-1 of pentagastrin was achieved by about 0.6 mg-kg-1h-1 of cimetidine. 2.4 mg-kg-1h-1 of cimetidine gave on average 90% inhibition. The inhibition caused by cimetidine decreased to a minimum of 57% and could not be overcome by increasing the doses of pentagastrin. The results suggest a mixture of competitive and noncompetitive interaction. The effect of cimetidine on pepsin secretion was less regular. Generally, pepsin output was less inhibited than acid output.
|
[
-0.0057535553351044655,
-0.008946174755692482,
-0.008917998522520065,
0.04568350315093994,
0.02150259166955948,
0.024112839251756668,
0.02546209655702114,
0.03339923545718193,
-0.036735355854034424,
-0.08462731540203094,
0.008753159083425999,
-0.017994362860918045,
0.0002716329472605139,
0.0313376821577549,
0.013452618382871151,
-0.016104649752378464,
0.06325790286064148,
-0.04187130182981491,
-0.07048261165618896,
-0.02299954742193222,
-0.043259598314762115,
-0.05284447222948074,
0.04176129400730133,
-0.00039623715565539896,
-0.03229321911931038,
0.012598278932273388,
0.010940064676105976,
-0.025623643770813942,
-0.08176323026418686,
-0.200712189078331,
0.05289769172668457,
-0.028309104964137077,
-0.003408033400774002,
-0.001002858392894268,
-0.06172759458422661,
0.0012695933692157269,
-0.03282294049859047,
0.04647889360785484,
0.04494465887546539,
0.0385386124253273,
0.06558598577976227,
0.03222067654132843,
-0.035822056233882904,
0.043163347989320755,
-0.031201746314764023,
0.010947603732347488,
-0.039039961993694305,
0.020468417555093765,
0.10036978870630264,
0.02302962727844715,
0.02403116039931774,
0.0020437969360500574,
-0.0033394095953553915,
0.09978528320789337,
0.004159442149102688,
-0.01220798585563898,
-0.039182133972644806,
0.026200246065855026,
0.016657698899507523,
0.007792983669787645,
-0.014883137308061123,
0.04789473116397858,
-0.1511576920747757,
0.033135101199150085,
0.039059486240148544,
0.02969757653772831,
-0.05110648646950722,
0.027325620874762535,
0.09516719728708267,
0.13955499231815338,
-0.03356210142374039,
0.03230773285031319,
-0.018712176010012627,
0.03062891773879528,
-0.005662023555487394,
-0.02575801871716976,
-0.05375581607222557,
-0.04849044978618622,
-0.004230036865919828,
0.06939221918582916,
0.0014214686816558242,
-0.002059890888631344,
-0.01730336807668209,
-0.017017673701047897,
0.024077948182821274,
-0.0423722080886364,
0.05142831802368164,
-0.007465291768312454,
-0.031755637377500534,
-0.008842774666845798,
0.01958024501800537,
-0.06341981887817383,
-0.06547041982412338,
-0.08702526241540909,
-0.06633060425519943,
-0.04134615510702133,
0.06479844450950623,
-0.035465143620967865,
-0.007353351451456547,
0.34623464941978455,
-0.0026709556113928556,
-0.04829484224319458,
-0.07334556430578232,
0.0055360812693834305,
0.014139711856842041,
0.0062009114772081375,
0.045854050666093826,
0.012293088249862194,
0.0008897908264771104,
0.016468780115246773,
0.07711648941040039,
0.023781122639775276,
0.020275311544537544,
-0.042957816272974014,
-0.04146954044699669,
0.05502266809344292,
0.07012704759836197,
0.020031090825796127,
0.017210504040122032,
0.033838022500276566,
-0.020385459065437317,
0.031986285001039505,
0.0013674276415258646,
0.007521164603531361,
0.004990719258785248,
0.014760366640985012,
-0.045199234038591385,
0.060781821608543396,
-0.003952354192733765,
-0.040934864431619644,
0.0351950041949749,
-0.03118184208869934,
-0.027134094387292862,
0.04865708947181702,
0.014562994241714478,
0.004510739352554083,
0.03332432359457016,
0.02672097645699978,
0.03072343021631241,
0.048597048968076706,
-0.008621027693152428,
-0.07508940249681473,
-0.06139141321182251,
-0.12448280304670334,
-0.0019379763398319483,
0.15270721912384033,
0.04936300963163376,
0.04270123317837715,
-0.045918215066194534,
-0.04420765861868858,
0.012793878093361855,
-0.003377967979758978,
0.029476899653673172,
0.00031020533060655,
0.005095913074910641,
0.03875454142689705,
-0.0015200944617390633,
-0.006267516873776913,
-0.005767647176980972,
0.007552378810942173,
-0.05203019827604294,
-0.04952496662735939,
-0.026288283988833427,
0.05600549653172493,
-0.00141454569529742,
-0.019100550562143326,
-0.03385887295007706,
-0.0039573474787175655,
0.0013363085454329848,
0.030235489830374718,
-0.0697406455874443,
0.014453081414103508,
-0.03591112419962883,
-0.01546423602849245,
-0.03826761990785599,
0.019651271402835846,
0.014279677532613277,
0.0037992424331605434,
0.05582008883357048,
0.005162297282367945,
0.03228870779275894,
0.038929834961891174,
0.005736253689974546,
0.023464420810341835,
-0.012418522499501705,
0.03053593821823597,
-0.01481516845524311,
0.02291467785835266,
0.028746530413627625,
0.037211984395980835,
-0.03228176385164261,
0.007081766612827778,
-0.08380287885665894,
0.026866629719734192,
-0.056561850011348724,
-0.041761163622140884,
-0.03887850418686867,
0.007528040092438459,
-0.048193398863077164,
-0.08503230661153793,
0.020095255225896835,
0.0423208624124527,
0.019464882090687752,
0.09012682735919952,
0.037313446402549744,
0.027075406163930893,
-0.02506212517619133,
-0.03820594772696495,
-0.0016744219465181231,
-0.031803928315639496,
0.026325713843107224,
0.007763166446238756,
0.014574969187378883,
0.009450473822653294,
-0.0682842880487442,
0.04972256347537041,
-0.025391386821866035,
0.0016971757868304849,
0.050714243203401566,
0.037816938012838364,
0.00894920527935028,
-0.036234695464372635,
-0.06298733502626419,
-0.31025320291519165,
-0.02197781577706337,
0.06684843450784683,
-0.026086293160915375,
0.09870775789022446,
-0.0729365423321724,
0.037121981382369995,
-0.03105936013162136,
-0.019116094335913658,
-0.05121993646025658,
0.04918355867266655,
0.0761699452996254,
-0.08573026210069656,
-0.04568991810083389,
-0.012130715884268284,
0.016208725050091743,
0.03218812867999077,
0.017437372356653214,
0.044602926820516586,
-0.040726225823163986,
-0.007802641950547695,
-0.007998677901923656,
0.04051646217703819,
-0.014818423427641392,
0.034935254603624344,
0.03526882082223892,
0.07143059372901917,
0.013367189094424248,
-0.06745344400405884,
-0.027899526059627533,
-0.018798600882291794,
0.038222797214984894,
-0.007058186922222376,
-0.09345817565917969,
0.032139454036951065,
-0.014284498058259487,
0.024476144462823868,
0.004508365411311388,
-0.008889125660061836,
-0.014121641404926777,
-0.05392032489180565,
-0.014673789963126183,
0.023559777066111565,
-0.05209869518876076,
-0.028522323817014694,
-0.029752003028988838,
-0.01981780119240284,
0.1224055141210556,
-0.06954658031463623,
-0.01142488420009613,
0.007951290346682072,
0.01936316303908825,
0.011288070119917393,
0.042233265936374664,
-0.014664805494248867,
-0.04164963215589523,
-0.03816770389676094,
0.028250856325030327,
-0.07038405537605286,
0.022386915981769562,
-0.03834285959601402,
-0.004641776904463768,
0.018539726734161377,
0.006724354345351458,
0.009449796751141548,
0.01019222941249609,
0.04186597466468811,
-0.018169522285461426,
0.04147951677441597,
0.03630826249718666,
0.09034216403961182,
0.11653285473585129,
-0.027295038104057312,
0.006403595674782991,
0.04451878368854523,
0.04574497789144516,
-0.03487784415483475,
-0.024349801242351532,
-0.09771779179573059,
0.023591341450810432,
-0.0033802748657763004,
0.017577851191163063,
0.029192952439188957,
-0.01649722270667553,
-0.06620113551616669,
-0.016501963138580322,
-0.0014393767341971397,
-0.02126127853989601,
0.060835059732198715,
0.021252378821372986,
0.020818036049604416,
0.03938416764140129,
-0.011721172370016575,
-0.012147612869739532,
0.012906004674732685,
0.03760490566492081,
-0.264708936214447,
0.026436200365424156,
0.04952578619122505,
-0.012471402063965797,
0.01344792265444994,
0.0507417693734169,
0.02980269119143486,
-0.04155516251921654,
-0.06054001674056053,
0.014391517266631126,
0.01174928992986679,
0.033138345927000046,
0.062438543885946274,
0.0620199479162693,
-0.03775661438703537,
-0.020155850797891617,
0.002238163724541664,
-0.035772208124399185,
-0.004335716832429171,
-0.03351408988237381,
0.008331403136253357,
-0.0015782314585521817,
0.11766891181468964,
-0.044906869530677795,
0.009370827116072178,
0.006839432753622532,
0.007740390487015247,
0.045918241143226624,
0.012401147745549679,
0.03029653988778591,
-0.047950830310583115,
0.03577141836285591,
0.013915426097810268,
-0.012721266597509384,
0.045188743621110916,
0.02992907166481018,
-0.014183690771460533,
-0.023962052538990974,
-0.05602440610527992,
0.017940228804945946,
-0.020257094874978065,
-0.01363298762589693,
-0.011199956759810448,
-0.03890583664178848,
0.05741708725690842,
-0.033515553921461105,
-0.004589737392961979,
-0.07856400310993195,
0.010519767180085182,
-0.02475719526410103,
-0.042361993342638016,
-0.09702765196561813,
0.07476994395256042,
0.05284982547163963,
0.027963021770119667,
0.011100961826741695,
0.01768752932548523,
-0.0349719375371933,
0.02161918580532074,
0.01621621660888195,
-0.03281399607658386,
-0.02926362305879593,
-0.008963408879935741,
0.008437913842499256,
0.030461328104138374
] |
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.